LOCUS       DBNGNK010000001         5191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1074707,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000001 DBNGNK010000000
VERSION     DBNGNK010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1074707"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="AC150V_00005"
     CDS             <1..192
                     /locus_tag="AC150V_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020107711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761337.1"
                     /translation="MRAEGCAFEELISFHGGMGGPQTRPFILAPVTLPPPGETILGAE
                     QVHRLLDGWRSSLIGTGES"
     gene            complement(189..932)
                     /locus_tag="AC150V_00010"
     CDS             complement(189..932)
                     /locus_tag="AC150V_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010355119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3761338.1"
                     /translation="MARIEYVGHATVLIDMDGVRILTDPLLRKRVTHLRRTGAVDESA
                     LQNIDAVLISHLHYDHLDKPSLRCLGKNTPMVMPRGSGRLLRREGFTTVTEIGVEEEI
                     SVGALRVRATPADHDQKRLPIGVRAEPVGYVIQGKQSVYFAGDTDLFEGMAMIGPVDV
                     GLIPNAGWGPGLGPGHMDAERAADSLPMLKPRVAIPIHWGTYYPAHLGLRGRPRFVDL
                     PPALFVRRAEEVAPDVDVRVLRPGEETSF"
     gene            998..2125
                     /locus_tag="AC150V_00015"
     CDS             998..2125
                     /locus_tag="AC150V_00015"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3761339.1"
                     /translation="MSASSAAGAAVDSVSPLRDQSRHRRLIKVAAWIGGIALLVLVLE
                     LLGVDVSGWLDELWEQLKAVPLWAFLGGVLLQVLQTTFNGVAYYGILRYAYPEAGVSF
                     WPIVTAYAVGVAGNSFLPANIGTFVTLLMFVAIIPGSTFPGILAAYLVNKIFFTVVGA
                     VVYVLLFLDAGAAFDVELGWFRDHPVLLLAIVVGGGFLVVFLVRIFWRWLKGLWEKAK
                     QGGAILSDPKAYATRVLVPQTLSYAAKVGVIAVFLAAYSIPVTFSSIVHVIGSSSAAN
                     VTSVTPGAVGVTQAANSVALRDFTDASTATAYSLSQQLVTSATNVVYALLLVVLIFGW
                     AGGKALVGDSYAGAKKKVHKKDKKNRDDAEGGGEGVDGAES"
     gene            complement(2353..3177)
                     /locus_tag="AC150V_00020"
     CDS             complement(2353..3177)
                     /locus_tag="AC150V_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3761340.1"
                     /translation="MSGTTRRTTMFESEFMRLAFAVGAVVGVLGPAVGFFLVQRRMSL
                     IGDGIGHVAFAGVAAGTLLGISPVLTALVAALIGAVGIEWLRARRLAAGDQALALVFY
                     TGIAAGVVLISIAGALDVNLFSYLFGSILTVTGTDVIVVAVLGAAGLFLLALFYRALT
                     GVIVDEEGARVAGLPIARLNMLVAGLAALTVALSMRVVGILLIAALMVLPVTAATRLG
                     WSLLSTLVLSTAIGLGSVLAGLTIAYYGDVPPGGTIVLVAAFAVLAALAGGRLRAA"
     gene            complement(3143..3862)
                     /locus_tag="AC150V_00025"
     CDS             complement(3143..3862)
                     /locus_tag="AC150V_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018823813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HIR3761341.1"
                     /translation="MSLTVELLGLSFGYERERPVLKNVDLRIATGEFVAIAGPNGGGK
                     TTLLRLVLGLERPDSGKAFLFGEPAPSSTRRSRLGYVAQRAHPGGDAPTTVRELVSAG
                     RLAAGPLLGPLRASDRKAVDRSIDRVGLSARADAPLSTLSGGMQQRAFIAKALAAEPS
                     LLVLDEPTTGVDAESQEALADLLSHLHGELGATVLYVSHEFGAVEPYVQRIVLVRGGI
                     VFDGPPEDLPYVWHDPSHDHV"
     gene            complement(3853..4698)
                     /locus_tag="AC150V_00030"
     CDS             complement(3853..4698)
                     /locus_tag="AC150V_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018787357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIR3761342.1"
                     /translation="MRARIILSFVAAVPLLAAAGAGCGGSAADESDQTVVAAFYPVAY
                     LAERLAPDAAVLNLTPAGAEPHDMALSPREVEAIRSADVVFYVGGGFMPAVEDAVAGS
                     DEGVDLLPSADPLPADPHAWLDPLRYAAMATAAAEALGAPGAEGELGGELEALDAEMR
                     AGLASCERRAIVTSHAAFSYLADAYDLEQVALAGLSPEAEPSARALEELVEEVRQSSA
                     TTVFTEPLASTRVAETVAREAGAETAVLNPLEGLTDDQLEAGDDYFSLMRENLETLRR
                     ALGCR"
     gene            4748..5185
                     /locus_tag="AC150V_00035"
     CDS             4748..5185
                     /locus_tag="AC150V_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR3761343.1"
                     /translation="MAGQTWVDHARVELRRAGHRSGGARDAVVLYLSARDCCVSAQEL
                     HDGLRGEGRRVGVASVYRSLEQLDELGLLHRVDLGDGVTRFEPARPDGEHHHHVVCDD
                     CGRVEQFSDPELERRLSSVAGGLGFELGEHDVVLHGSCLDCRD"
BASE COUNT          796 a         1763 c         1840 g          792 t
ORIGIN      
        1 atgcgcgccg agggctgcgc gttcgaggag ctgatctcgt tccacggtgg catgggcggt
       61 ccccagacca ggcccttcat cctcgctccc gtcacgctcc cgccgccggg ggagacgatt
      121 ctcggcgcgg agcaggtgca ccgcctgctc gacggctggc gctcgagcct catcgggacc
      181 ggcgagtcct agaagctcgt ctcctctccg ggcctgagaa cacgtacgtc gacgtcgggc
      241 gcgacctcct cggcccgccg gacgaagagt gcaggaggga gatcgacgaa gcgtggccgc
      301 ccgcggagcc cgagatgagc tgggtagtac gtcccccagt ggatcgggat cgccacacga
      361 ggcttgagca tcggcagcga gtcggccgcc cgctccgcgt ccatgtggcc ggggccgagg
      421 cccggccccc agccggcgtt ggggatcagc ccgacgtcga ccggcccgat cattgccatc
      481 ccctcgaaga ggtccgtgtc gccggcgaag tacacgctct gcttcccctg gatcacgtac
      541 cccaccggct ccgccctgac gccgattggt agccgcttct ggtcgtgatc tgccggcgtc
      601 gcgcgcaccc ggagagcgcc gaccgagatc tcctcctcga cgccgatctc ggtcacggtc
      661 gtgaagccct cgcggcggag aagccggccg gacccacgcg gcatcaccat cggcgtgttc
      721 ttgccgaggc accgtagtga gggcttgtcg aggtggtcgt agtggagatg ggagatgagc
      781 acggcgtcga tgttctgcag cgcgctctcg tcgaccgccc cggtacggcg caggtgcgtc
      841 acccgcttcc gcaggagcgg gtccgtgagg atccgcaccc cgtccatgtc gatcagcacg
      901 gtggcgtggc cgacgtactc gatccgggcc atcagagcga tcctagattc gggtctcttc
      961 tctatgctcg cgcgatggtc gacgcggggc cgacggtatg agcgcctcga gcgctgccgg
     1021 cgcggccgtc gacagcgtca gcccgcttcg cgaccagtcg cgccatcgcc ggctgatcaa
     1081 ggttgccgcc tggatcggtg gcatcgcgct gctcgtgctc gtactcgagc tcctcggtgt
     1141 cgacgtcagc ggctggctgg acgagctctg ggagcagctc aaggcggttc ccctctgggc
     1201 gttcttgggc ggcgtcctgc tgcaggtgct gcagacgacg ttcaacggcg tcgcgtacta
     1261 cgggatcctc cgctacgcct atccggaagc cggcgtcagc ttctggccga tcgtcacggc
     1321 ctacgcggtg ggcgttgcgg ggaacagctt cctaccggcc aacatcggca ccttcgtcac
     1381 gttgctgatg ttcgtcgcga tcattcccgg ctcgaccttt ccgggcatcc tcgccgccta
     1441 cctcgtcaac aagatcttct tcacggtcgt cggagcggtc gtgtacgtgc tcctcttcct
     1501 ggacgcaggg gccgcattcg acgtcgagct gggctggttc cgcgatcacc ccgtcctcct
     1561 gctggccatc gtcgtgggcg gcggatttct ggtcgtcttc ttggtgcgca tcttctggcg
     1621 ctggctgaaa gggctctggg agaaggcgaa acaaggtggg gcgatcctca gcgacccaaa
     1681 ggcgtacgcg acgcgtgtgc tcgtgccgca gacgctgagc tacgcggcga aggtcggcgt
     1741 gatcgccgtc ttcctcgccg cgtactcgat cccggtcacg ttcagctcga tcgtccatgt
     1801 gatcggctcg agctcggcgg cgaatgtgac ctcggtcacg cccggagcgg tcggcgtgac
     1861 acaggcggcg aacagcgtcg ctctgcggga tttcacggat gcctcgacgg cgacggccta
     1921 ctcgctgagc caacagctgg tcacctcggc gacgaacgtc gtctacgcgc tccttctcgt
     1981 cgtactcatc ttcggctggg cggggggaaa ggcgctcgtc ggcgactcgt acgcgggcgc
     2041 caagaagaaa gtgcacaaga aggacaagaa gaaccgcgac gacgccgagg ggggcgggga
     2101 aggagtcgac ggcgcagagt cgtagtcggc ccgcccacga tccagacgaa ggcaacgaac
     2161 atctgggcct ccctcgtcat cacgagcgtg acgatcttcg gggcctgctc ggtgttttcg
     2221 gtgcgatcgt ggccgtcccc tgacggcgtc gatccagctc gtcatccagg agatcaccgc
     2281 cgataggcgc gagcggatgg ccgcgttgaa acctgcccag gccgctaggg ccgaggcgcc
     2341 gaccggtggt gcctaggcgg ctcgaagacg gccacccgcc agcgcggcga gaacggcgaa
     2401 cgcagcgacc aggacgatcg tgccgcccgg agggacgtcg ccgtagtacg cgatcgtgag
     2461 tccggcgagg acggagccga ggccgatcgc cgtggagagg acgagcgtcg aaagcaggct
     2521 ccagcccagt cgggtcgcgg ccgtcaccgg aagcaccatc agcgccgcga tcaggagaat
     2581 tccgacgacg cgcatcgaca gcgcgaccgt cagcgcggcg agcccggcaa cgagcatgtt
     2641 cagcctggcg atcggaaggc cggcgacgcg cgcgccctcc tcgtcgacga tgacgccggt
     2701 cagcgcgcgg tagaagagcg cgagcaggaa caggccggct gccccgagca cggcgaccac
     2761 gatgacgtcg gtgcctgtga cggtgagaat cgagccgaac aggtatgaga agaggttgac
     2821 gtcgagtgcg ccggcgatgg agatcaggac gacgccggct gcaattccgg tgtagaagac
     2881 gagcgcgagc gcttggtcgc cggcggcgag ccggcgcgcc cgcagccact cgattccgac
     2941 ggccccgatc agggcggcga cgagcgctgt gagaacaggg gagatgccga gcagcgtgcc
     3001 ggccgcgact ccggcgaacg cgacgtgccc gatcccgtcg ccgatcaggc tcatcctgcg
     3061 ctggacgagg aagaagccga ctgcggggcc gaggacgccg acgaccgccc cgacggcgaa
     3121 ggccagccgc atgaactcgc tctcaaacat ggtcgtgcga cgggtcgtgc cagacatacg
     3181 ggagatcctc cggcgggccg tcgaagacga ttccccctcg tacgaggacg atacgctgca
     3241 cgtagggctc gacggcgccg aactcgtgcg acacgtagag aacggtcgcc ccgagctcgc
     3301 cgtggaggtg tgagagaagg tcggccagcg cctcctgcga ctcggcgtca acccccgtcg
     3361 tcggctcgtc gagtacgagc agtgaaggct cggcggcgag tgccttggcg atgaaggctc
     3421 gctgctgcat gccgccggag agcgtcgaga gcggcgcgtc ggcccgcgcc gacaggccga
     3481 cgcggtcgat ggagcggtcg accgccttgc ggtcggaggc gcggagcggg ccgagcagcg
     3541 ggcctgccgc cagcctgccc gccgatacga gctcgcggac ggtcgtcggc gcatcccctc
     3601 cggggtgcgc ccgctgggcg acgtagccga gtcggcttcg ccgggtcgag ctgggcgccg
     3661 gctcgccgaa caggaatgcc ttcccggagt cgggacgttc gagcccgagg acgagtcgca
     3721 gcagcgtcgt cttgcccccg ccgttcggcc ctgcgatcgc tacgaactca ccggtcgcga
     3781 tccgaaggtc gacgttcttg agcacgggac gctcgcgctc gtagccgaac gacagcccga
     3841 gcagctcgac ggtcagcgac atccgagggc ccgccgcagt gtctccaggt tctcgcgcat
     3901 gaggctgaag tagtcgtccc cggcttcgag ctggtcgtcc gtcagcccct cgagcgggtt
     3961 cagcacggcc gtctctgcgc ccgcttcccg cgcgacagtc tcggccacgc gcgtcgacgc
     4021 gagaggctcg gtgaataccg tcgtcgccga cgactgccga acctcttcga cgagttcctc
     4081 gagcgcgcgt gcactgggct cagcctccgg cgacagtccg gcgagcgcca cctgctcgag
     4141 gtcgtaggcg tcggcgaggt aggagaaggc cgcgtggctc gtgacgatcg cgcggcgttc
     4201 gcacgaggcg aggcccgctc gcatctccgc gtcgagcgcc tcgagctcgc cgccgagctc
     4261 cccctcggcg ccgggcgcgc cgagcgcctc ggccgccgcg gtcgccatgg ccgcgtagcg
     4321 cagaggatcg agccaggcgt gcgggtcggc ggggagcggg tcggcgctcg gcagcaggtc
     4381 gacgccctcg tcgcttccgg ccactgcgtc ctccacggcg ggcatgaagc cgccgcctac
     4441 gtagaacacg acgtcggctg aacggatcgc ctcgacctct cgcggagaga gcgccatgtc
     4501 gtgcggctcg gctccggccg gcgtgaggtt cagcaccgcg gcgtcgggtg cgagccgttc
     4561 ggcgagatag gcgaccgggt agaacgctgc gacgaccgtc tggtcggact cgtccgccgc
     4621 gcttccgccg cagccggctc ccgcagccgc gagcagcggg acggcggcga cgaagctgag
     4681 aatgattctc gcccgcacag gggagggaag cgtagcactt cttcgataac ggttactatt
     4741 ctcagggatg gccgggcaga cgtgggtcga tcacgcgcgc gtcgagctac gccgcgcggg
     4801 gcatcgcagc ggcggcgccc gcgacgccgt ggtgctgtat ctctccgccc gcgactgctg
     4861 cgtgagcgcg caggagctgc acgacggact gcgcggggaa ggtcggcgcg tcggggtcgc
     4921 gagcgtctat cggtcgctcg agcagctcga cgagctaggg ctgctccacc gcgtcgacct
     4981 cggcgacggc gtcacgcgct tcgagcccgc tcgcccggac ggcgagcacc accaccacgt
     5041 cgtctgcgac gactgcggcc gcgtcgagca gttcagcgat cccgagctcg agcggaggtt
     5101 gtcgtccgtc gccggcggac taggtttcga gctcggcgag cacgacgtcg tgctccacgg
     5161 ctcctgtctg gactgccgcg actgagcgcg g
//
LOCUS       DBNGNK010000002         2722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_788412,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000002 DBNGNK010000000
VERSION     DBNGNK010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_788412"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1018
                     /locus_tag="AC150V_00040"
     CDS             <1..1018
                     /locus_tag="AC150V_00040"
                     /inference="COORDINATES: protein motif:HMM:NF036799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR3761344.1"
                     /translation="AEKQRGLLERLGFEVTDDWDVTVPSWRARDVWREVDLIEEVARA
                     VLDDVPLTVPLRRHVQGRLSKDQRLRRVVEDVLVGAGFSEAYTWSLQPDDPAEDAIRL
                     PDPMTSEQAVLRTTLVSGLIEAARTNVDAANEEIALFEIARVYLPDGGGLPNERLLVG
                     GVVEGGFSAVKGAVEAVYGALHLEPDPARAVHPLLHPGKAARLGAGWVGELHPTLLEG
                     EWGAFELDLATLFEPVPERIVYEDVITYPALRQDIAVVVPENVEAGTLVEVAREAAGD
                     ELREAHVFDVYRGGQVGPGQKSVALRLSFQSPERTLSDDDAATLRGRIVQALAERFGA
                     ELRA"
     gene            complement(1028..1675)
                     /locus_tag="AC150V_00045"
     CDS             complement(1028..1675)
                     /locus_tag="AC150V_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761345.1"
                     /translation="MKLADQWAAIEANLPQDWEEVRLVLRTEQKDELDRAAAVLGSLS
                     AGRSGETLVFQVRRAGGGAGPEGARRLFRRLDVERVWCRLEETGVERAATDGAQHTEL
                     APLVEQFDAALGSLPSDWSDLLGEVEISSSAWLDRAALLCAPINPSRSREALALLFRC
                     ARRSGYGASAGMARRCLERLDGEGISGSVRVLRALSETDPVGTQGPVFPIDGRVL"
     gene            1699..2715
                     /locus_tag="AC150V_00050"
     CDS             1699..2715
                     /locus_tag="AC150V_00050"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3761346.1"
                     /translation="MARVEGTSSVHTRGLNEAQLRDLFREMLVIRRFEEAVEERFRAG
                     ELPGFLHVALGEEAVASGVCAALESDDVIASTHRAHHHTIAKGTSINAVMAELYGKVE
                     GCSGGFGGSMHLYDVSVGNLGANAVVGGGIPGIVGAALAFKLRGEPRVAVAFFGDGAT
                     NTGAFHESLNLAQLWSVPALLVLENNGWAESTPATQQLPLPVDELGQRAAAYGMHLIE
                     ADGQDVEAVYDATARAREHAVSGQGPVFLHIRTHRLTGHYVGDPQVYRDKDELRQLWE
                     KVDPIELLRQRLVISIDEFDAIDAEVSAEVEACVEFAKNGTDPEGEDSLKWVYAEGSL
                     APDA"
BASE COUNT          421 a          924 c          970 g          407 t
ORIGIN      
        1 tgcagagaaa cagcgcggac tgctcgagcg gctcggtttc gaggtgaccg acgactggga
       61 cgtgaccgtg ccgagctggc gcgcgcgtga cgtctggcgg gaggtcgacc tgatcgagga
      121 ggtcgcacgc gcggtgctcg acgatgtgcc gctcacggtt ccgctgcgac ggcacgtcca
      181 aggccggctc tcgaaggatc agcgcctgcg tcgcgtcgtc gaggacgtcc tcgtcggcgc
      241 cggcttctcc gaggcgtaca cgtggagtct ccaaccggac gacccggcgg aggatgcaat
      301 ccggctgccc gatccgatga cgtcagagca ggcggtcttg cgaacgacgc tggtgagcgg
      361 cctgatcgag gccgcgcgca ccaacgtcga tgcggccaac gaggagatcg cgctcttcga
      421 gatcgcgcgc gtctacctgc cggacggcgg cggcctgccg aacgagcgtc tcctcgtcgg
      481 cggggtggtc gagggcggct tcagcgccgt caagggagcc gtcgaagccg tgtacggcgc
      541 actccacctc gagcctgacc cggcgcgcgc cgtccacccg ctccttcatc ccggcaaggc
      601 ggctcgactc ggggccggct gggtcggcga gcttcacccg acgctcctcg aaggggagtg
      661 gggcgcgttc gagctcgatc tggccaccct gttcgagccg gttcccgaac ggatcgtgta
      721 cgaagacgtc atcacctatc cggcactgcg gcaggacatc gccgtcgtgg tgccggagaa
      781 cgtcgaggcc ggaacgctgg tcgaggtcgc ccgcgaggct gccggcgacg agctccgcga
      841 ggcgcacgtg ttcgacgtgt atcggggcgg ccaggtcggg ccggggcaga agtcggtcgc
      901 cctgcgactc tcgttccagt cgcccgagcg gacgctctca gacgacgacg ccgcgacgtt
      961 gcgcggccgc atcgtccagg cgcttgccga gcgattcggg gccgagctcc gagcctagcc
     1021 cgtcgagcta gagaactcgg ccgtcgatcg ggaagaccgg cccttgtgtc cccaccggat
     1081 cggtctccga cagcgcccgc aggacgcgca cggagccgct gatgccctcc ccgtcgagtc
     1141 gctccaggca gcggcgcgcc atcccggctg aggcgccgta gccggagcgg cgtgcgcaac
     1201 ggaagagcag cgcgagcgcc tcacggctgc gagaagggtt gatcggggcg cagagcagcg
     1261 ccgcgcgatc gagccaggcg ctcgagctga tctcgacctc gccgagcaga tcactccagt
     1321 ccgacgggag actgcccagc gcagcgtcga actgctctac gaggggcgcc agctcggtgt
     1381 gctgcgcgcc gtcggtcgcg gcgcgctcta cacccgtctc ctcgagacgg caccacacac
     1441 gttcgacgtc gagtcggcgg aacagcctgc gcgcaccttc ggggcccgcg ccgccgcctg
     1501 cccgcctgac ctggaagacg agggtctcgc ccgaccgacc cgcgctcagc gagccgagca
     1561 ctgcggccgc ccggtcgagt tcgtcctttt gctcggttct gaggactagg cgcacctcct
     1621 cccagtcctg cggcaggttc gcctcgatcg ccgcccactg atccgcgagc ttcacggctg
     1681 tgagcataat gacgcgcgat ggccagggtc gaaggcacct caagcgtgca tacgcgcggg
     1741 ctgaacgagg cgcagctgcg ggatctcttc cgagagatgc ttgtgatcag gcggttcgag
     1801 gaggcggtcg aggagcgttt ccgcgccggc gagcttcccg ggttcctcca cgtggcgctc
     1861 ggcgaggagg ccgtcgcgtc aggtgtgtgc gccgcgctgg agtccgacga tgtcatcgcc
     1921 tcgacgcatc gtgcccacca ccatacgatc gcgaagggaa cgagcatcaa cgccgtgatg
     1981 gccgagctgt acgggaaagt cgagggatgc tcgggcggct tcggcggctc aatgcacctc
     2041 tacgacgtct cggtcgggaa tctgggcgcc aacgccgtcg tcggcggcgg cattccgggg
     2101 atcgtcggtg cggcgctcgc cttcaagctg cggggcgagc cgcgcgtcgc ggtcgcgttc
     2161 ttcggcgacg gcgcgaccaa cacgggagcc tttcacgagt cactcaacct cgcgcagctc
     2221 tggagcgtgc ccgcgctgct ggtgctcgaa aacaacggct gggcggagtc gacgccggcc
     2281 acgcagcagc ttccgctccc ggtcgacgag ctcggacagc gggccgcggc gtacggcatg
     2341 cacctgatcg aggccgacgg ccaggatgtc gaagcggtct acgacgccac cgcgcgcgcc
     2401 cgcgagcacg ccgtctccgg tcaggggccg gtgttcctcc acatccggac gcaccggctc
     2461 acgggccact acgtcggcga cccgcaggtg tatcgagaca aggatgagtt gcggcagctg
     2521 tgggagaagg tcgacccgat cgagctcctg cgccaacggc tggtgatctc gatcgacgag
     2581 ttcgatgcga tcgacgcgga ggtctccgcc gaagtcgagg cgtgtgtcga gttcgccaag
     2641 aacggcaccg acccggaggg cgaggactcc ctgaagtggg tctatgccga gggaagtctg
     2701 gcgccggatg cctgagctga cc
//
LOCUS       DBNGNK010000003         3314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1791530,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000003 DBNGNK010000000
VERSION     DBNGNK010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3314
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1791530"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="AC150V_00055"
     CDS             <1..808
                     /locus_tag="AC150V_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3761347.1"
                     /translation="SAYGFPLYWTLTMAFKPQDEWNPPGQVFWWPDNWTLSNFTDILG
                     LQEATGFATGRSRSATEFLLNSAIASIAGTLLAAVLGVLAAYGIARWRAGGRILPFQI
                     LQLRMFPPIAILIPLLFFFVYLNLWDTLWGLSLIYAAITFPFVVWLMRSFFQEVPREL
                     TDAAIVDGCTHWGAFFKVVLPQVKGGLAATALFVFILNWSDFLIALVMSQDNAKTAPV
                     YLQSLQSGSAGQEYGKQAALAMILILPPAIFGLAIQKYLVRGLTFGAIKR"
     gene            819..1856
                     /locus_tag="AC150V_00060"
     CDS             819..1856
                     /locus_tag="AC150V_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761348.1"
                     /translation="MAQIHLEHLTKKFPGGVLAVDDMELVIPDGSFVALLGPSGCGKT
                     TTMNMIAGLEKPTGGKIYFDDVEVTRLDPGDRTVGFVFQNYAIFTHMTVWENLAFGLR
                     VRKPKPSKQVVDQEVRRVAGVLGITQYLDRNAARLSVNDMQKVALGRSMIVQPAIFLL
                     DEPFSNLDAAFRAYMRAQLKEIQHQLGQTMIYVTHDQVEAMSMADQIAVMNLGVLQQY
                     ATPDVLYNKPANRFVAGFVGSTQMNFIPAGIARIDRPDTNGRSLTLGIRPEYVTIGPP
                     EAEDARLRASVNLVEPLGAKDVVHLTADGHDFRVVSTPSRRPKVGENVGLAFEESRML
                     LFDDETGEAVS"
     gene            1860..2996
                     /locus_tag="AC150V_00065"
     CDS             1860..2996
                     /locus_tag="AC150V_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761349.1"
                     /translation="MASVKLENVTVRYGNVTALDGVSLEISDGEFFAVLGPPGAGKTT
                     LLRTIVGLEKPVTGDVFADDENITQMYPGDRGIAIMFQNLALYPDRTVFDNLAFPLKQ
                     AKWKKGDIRQRVHEVAGVLHIDPLLKRKPGKLSGGERQRVALGRAMVRDPRVLLLDEP
                     LSALDALLRLEMRVELKRLQRDLKRTLVYVTHDQVEAMSMPDRVAVLGNGLVQQVDTP
                     ENVYNRPTNQFTARVIGSPPINFIPCKASRANGGVQVVHSGFAVDVLDTAGTGGALAE
                     GADCVLGVRPEDVRVCEPGEDGIDAEVSVTEPLGGETVIDLHLGDLLIKALVPPTVSP
                     AEGSRVKVAFDTQRIHVFGSAGDTLVSAGGADGVFTVAPRHPVA"
     gene            complement(3010..>3314)
                     /locus_tag="AC150V_00070"
     CDS             complement(3010..>3314)
                     /locus_tag="AC150V_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025397.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3761350.1"
                     /translation="DRATIDGGSKTFSGDVRVAREGAVEPTAVARAADRPAYVERVTE
                     EHGVIDEGAEALGLGERVAFYPAHVCTCVNLSDVLYGVRDGLVAEAWPVLARGKRT"
BASE COUNT          541 a         1162 c         1070 g          541 t
ORIGIN      
        1 cagcgcgtac ggattccctc tctactggac gctgacgatg gcgttcaagc cgcaggacga
       61 atggaacccg cccgggcagg tgttctggtg gccggacaac tggacgctct cgaacttcac
      121 cgacatcctc ggcctccaag aggcgaccgg cttcgcaacc ggaaggtctc ggtcggcgac
      181 cgagttcctc ctgaacagcg ctatcgcatc gatcgcaggg acgctcctgg cggctgtgct
      241 cggcgtgctc gccgcctacg gcatcgcccg ctggcgcgcc ggcgggcgca ttctcccctt
      301 ccagatcctc cagctgcgca tgttcccgcc gatcgcgatc ctgatcccgc tgctcttctt
      361 cttcgtctac ctcaacctct gggacacgct ctggggactg agcctgatct acgccgcgat
      421 cacgttcccg ttcgtcgtct ggctcatgcg gagcttcttc caggaggtgc cacgcgagct
      481 caccgacgcc gcgatcgtcg acggctgcac gcactggggc gccttcttca aggtcgtgct
      541 cccccaggtg aagggaggac tcgctgcgac ggcgctcttc gtcttcatcc tcaactggtc
      601 cgacttcctc atcgcgctcg tcatgtcgca ggacaacgcc aagactgccc ccgtctacct
      661 ccagtcgctc cagtcgggca gcgcaggcca ggagtacggc aagcaggccg ctctggcgat
      721 gatcttgatc ctcccgcccg cgatcttcgg gctggcgatc cagaagtacc tcgtgcgcgg
      781 gctcacgttc ggggcgatca agcggtagtg cctcggtaat ggcccagatc catctcgagc
      841 acctgacgaa gaagttcccc ggcggggttc tcgccgtcga cgacatggag ctcgtcattc
      901 ccgacggatc gttcgtcgcg ctgctcgggc cgtccggctg cggcaagacg acgacgatga
      961 acatgatcgc gggactggag aagccgaccg gaggcaagat ctacttcgac gacgtcgagg
     1021 tgacgaggct cgacccgggt gaccgcaccg tcggcttcgt cttccagaac tacgcgatct
     1081 tcacgcacat gacggtgtgg gagaacctcg cgttcggcct ccgcgtgcgc aagcccaagc
     1141 cgtcgaaaca ggtggtcgac caggaggtgc ggcgggtcgc cggcgtcctc gggatcaccc
     1201 agtatctcga ccggaacgcc gcccgtctgt cggtgaacga catgcagaag gtcgctctcg
     1261 gccggagcat gatcgtgcag cccgccatct ttctgctcga cgagccgttc tcgaacctcg
     1321 acgccgcttt ccgcgcctac atgcgggcgc agctgaagga gattcagcac cagctcggcc
     1381 agacgatgat ctacgtcacc cacgaccagg tcgaggccat gagcatggcg gaccagatcg
     1441 ccgtcatgaa cctgggcgtc ctccagcagt acgcgacccc ggacgtgctc tacaacaagc
     1501 cggccaaccg cttcgtggcg ggattcgtcg gctcgacgca gatgaacttc atccccgccg
     1561 ggatcgccag gatcgaccgg cccgacacga acgggcgatc gctgacgctc ggcatccggc
     1621 ccgagtacgt cacgatcggt ccgccagagg cggaggacgc gaggctccgc gcctccgtga
     1681 acctggtcga gccgctcggg gccaaggacg tggtgcacct gacggccgac ggccacgact
     1741 tccgtgtcgt cagcacaccg agtcgcaggc cgaaagtcgg cgagaacgtg gggctggcgt
     1801 tcgaggagtc ccgcatgctc ctcttcgacg acgagaccgg cgaggcggtg agctaggcag
     1861 tggcctctgt caagctcgag aacgtcaccg tccgctacgg gaacgtcacg gcgctcgacg
     1921 gcgtgtcgct cgagatctcc gacggggagt tcttcgcagt cctcggccct cccggcgcgg
     1981 ggaagacgac gctgctgcgc acgatcgtgg gcctcgagaa gcccgtgacg ggagacgtct
     2041 tcgccgatga cgagaacatc acccagatgt atccgggcga ccggggcatc gcgatcatgt
     2101 tccagaacct cgccctctac ccggaccgca ccgtcttcga caacctcgcg ttcccgctca
     2161 agcaggcgaa gtggaagaag ggcgatatcc gccagcgcgt gcacgaggtc gccggcgtgc
     2221 ttcacatcga tccgctcctc aagcgcaagc cgggcaagct ctccggcggc gagcgccagc
     2281 gcgtcgcgct cgggcgggcg atggttcgcg atccccgcgt cttgctcctc gacgagccgc
     2341 tctcggcgct cgacgcgctg ctgcgcctcg agatgcgcgt cgagctcaag cgcctccagc
     2401 gcgacctgaa gcgcacgctc gtctacgtca cgcacgacca ggtcgaggcg atgagcatgc
     2461 cagaccgcgt ggcggtgctc gggaacggac tggtgcaaca ggtcgacacg cccgagaacg
     2521 tctacaaccg gccaacgaat cagttcacgg cgagggtgat cggcagcccg ccgatcaact
     2581 tcatcccgtg caaggcgagc agggcgaacg gcggcgtcca ggtcgtgcat tccggcttcg
     2641 cggtcgacgt cctcgacacg gcggggacgg gaggcgccct cgccgagggg gccgactgcg
     2701 tgctcggagt ccgccccgag gacgtgcggg tgtgcgagcc gggcgaggac ggcatcgatg
     2761 ccgaggtctc ggtcaccgag ccgctcggcg gcgagaccgt gatcgatctc cacctcggcg
     2821 acctcctcat caaggcgttg gtcccgccga ccgtatcccc ggccgagggc agccgcgtga
     2881 aggtcgcttt cgacacccag cgcatccacg tcttcggctc ggccggcgac acgctcgtct
     2941 cggcaggcgg cgcggacggc gtcttcacgg tcgcgccgcg tcacccggtc gcgtaggagc
     3001 tgtccgcctc tacgtccgct taccgcgggc gagcaccggc cacgcctccg cgacgaggcc
     3061 gtcgcgcacg ccgtagagca cgtcggagag gttgacgcag gtgcatacgt gcgctgggta
     3121 gaacgcgact cgctccccga gcccgagtgc ttcggcaccc tcgtcgatga cgccgtgctc
     3181 ctcggtcacg cgctccacgt aggcgggcct gtcggcagcg cgggcaacgg cagtcggctc
     3241 gactgctccc tcccgagcca cgcgcacgtc tccgctgaag gtcttcgagc cgccgtcgat
     3301 ggtcgcccgg tcgc
//
LOCUS       DBNGNK010000004         1884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1433230,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000004 DBNGNK010000000
VERSION     DBNGNK010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1433230"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC150V_00075"
     CDS             complement(<1..474)
                     /locus_tag="AC150V_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HIR3761351.1"
                     /translation="MKKVLLTVAAGLLLAAGAVLAAAVSGPSEAGAADHVDAPGLTPP
                     GGNFQLDIADLYAWRARSGNTVLAMTVNGLTKAKANPVFASGVPGVRATRGVSYWFRV
                     DNDGDAVPDVNLQVTFGKPNAKGVQKMTVRRNGKVLVSGESTRPGAKFQKVSKGNG"
     gene            644..1216
                     /locus_tag="AC150V_00080"
     CDS             644..1216
                     /locus_tag="AC150V_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3761352.1"
                     /translation="MRANVAHLSDEALVALVARGDNTALGELYDRIGRIAYSLALRVL
                     RDERLAEDAVQEGFLAAWRGAASFSAERASARTWILTFVHRRAVDLVRRSERRRTQPI
                     EEASERATGAAAATDDEVWLRLERERVQAALQRLSDDQREAIELAYYGGYSQSELAEK
                     LGVPLGTIKSRMFAGLTRLRELLDDHEGMG"
     gene            1213..>1884
                     /locus_tag="AC150V_00085"
     CDS             1213..>1884
                     /locus_tag="AC150V_00085"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR3761353.1"
                     /translation="MTHGDIHDLTAAFALDALDLEERAEYEEHLQTCERCREELASFS
                     TVTGALAAAAAGPAPRAELRSRILEEAQADGAKVVPFTRPRRAPLIAVGAVAAVAAAV
                     AIGLGIWALSTSQELDDTRLALAQQEAATAVLADPEARSVPLQTGAGFLVVDSAGRGV
                     LVLDSLAPAPNGSSYQIWVIEGNSPTPAGLFSASTRVTVAPLGERVPESAVVAVTVEP
                     DGGSQA"
BASE COUNT          278 a          650 c          656 g          300 t
ORIGIN      
        1 gccgttgccc ttgctgacct tctgaaactt cgcaccagga cgggtcgact cgccgctcac
       61 gagcaccttg ccgttccggc ggacggtcat cttctgtacg cccttcgcat tcggcttgcc
      121 gaacgtcact tgcaggttga cgtcgggaac ggcgtcgccg tcgttgtcga cgcggaacca
      181 gtaggacacg cccctcgtcg cgcgcacgcc gggaacgccg ctcgcgaaga ccggattggc
      241 ctttgctttc gtgagcccgt tgaccgtcat ggccagaacg gtgttcccac tccgggcccg
      301 ccaggcatag aggtcggcaa tgtcgagctg gaagttgccg cccgggggcg tcaggccggg
      361 ggcatcgacg tggtcggcgg cccccgcttc actcgggccg ctgaccgccg ctgcgagcac
      421 cgcgccggcg gccagcagga ggcctgcggc aacggtgagc aagactttct tcataaccca
      481 ctccttcggt ggagatcgat tgacgggcgt tgttcgcccc cgggcgccga tcggttcgaa
      541 ccgttgccgc ggcgcgtgcg aatcaccacc gatgacggtg acggcaaccg tgatagattc
      601 ccgccgcgtt acgcctgagc gggcgcggat tccaacgctc tcgatgcgag cgaacgtcgc
      661 ccatctctcc gacgaggccc tcgtggccct ggttgctcgg ggcgacaaca ccgccctggg
      721 cgagctctac gatcggatcg gccggatcgc ctacagcctc gcgctccgcg tcctgcggga
      781 cgaacgactc gccgaggatg cggtgcagga agggttcctc gcggcatggc gcggcgcggc
      841 gagtttctcg gccgagcgcg cgtcggcacg cacgtggatc ctcacgttcg tccaccggcg
      901 cgcggtggat ctcgtgcggc ggagcgagcg gcgtcgtacc caaccgatcg aagaagcctc
      961 cgagcgtgcg accggtgctg ctgcggcaac cgacgacgag gtgtggctgc ggctggagcg
     1021 cgagcgtgtc caggcggccc tacagcgcct ttcggacgac cagcgcgagg cgatagagct
     1081 cgcctattac ggcggctatt cgcagtccga gctggccgag aagctcggcg tgcccctcgg
     1141 tacgatcaag agccggatgt tcgccggtct cactcgcttg cgcgagctac tcgacgacca
     1201 cgagggaatg ggatgactca cggcgacatc catgatctga ccgcggcctt cgcgctcgac
     1261 gcactcgatc tcgaggagcg ggccgagtac gaggaacacc tgcagacgtg cgagcgctgc
     1321 cgcgaggagt tggcgtcgtt ttcgaccgtc accggggcac tcgcagccgc cgcggcaggg
     1381 ccggcgcccc gcgcggagct ccgctcccgg atcctcgagg aggcacaggc cgacggagcg
     1441 aaggtcgtgc cgttcacgcg accacgccgg gctcccctga tcgcggtcgg agcggtggcg
     1501 gctgtggctg cagccgtcgc gatcggactg ggtatctggg cgctctccac ctcgcaggag
     1561 ctcgacgaca cgcgccttgc gctcgcgcag caggaggctg cgaccgccgt actcgccgac
     1621 ccggaggcgc gcagcgtgcc gctccagacg ggtgcaggct tcctcgtcgt cgactccgca
     1681 ggccgtggcg tgctcgtgct cgactcgctg gcgccggccc cgaacggcag cagctaccag
     1741 atctgggtga tcgagggcaa cagcccgacg cctgcgggat tgttctctgc gtcgacgcgc
     1801 gtgaccgtcg ctccactcgg cgagcgcgtg cccgagagcg cggtcgtcgc cgtgacggtg
     1861 gagcccgacg gcggatcaca ggcg
//
LOCUS       DBNGNK010000005         3916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_287011,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000005 DBNGNK010000000
VERSION     DBNGNK010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_287011"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1530
                     /locus_tag="AC150V_00090"
     CDS             <1..1530
                     /locus_tag="AC150V_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018937682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIR3761354.1"
                     /translation="LSWGHLWEQCRLDIGAGERPEQILQLHVFHLLQTVSPHTIELDA
                     GVPARGLHGEAYRGHIFWDELFIFPFLNLRLPGITRALLRYRFRRLPEARWAAQQAGF
                     DGAMYPWQSGSDGREESQTWHVNPASGHWLADHSHLQRHIGVAVAYNVWLYYQTTGDL
                     DFLAYWGAEMMLEIARFFASASTYDRSRDRYEIKNVMGPDEYHEAYPGAAEPGLDNNA
                     YTNVMAAWVLTRALEVLDLVPEIRRQRLWEKLGLTREELDKWEGISRKMLIPFHDEGI
                     ISQFEGYGELAEFDWDGYRERYGDISRLDRILESEGDSPNNYKLSKQADVLMLFYLLS
                     GEELAGVFERLGYTFDDNLIPRNVEYYTRRTSHGSTLSRVVNGWVLSRLDRRQSWEFF
                     MAALESDVSDVQGGTTREGIHLGAMAGTVDLVQRCFGGLETREDVLWLDPQLPPELGR
                     LRFDVRYRNSWLSIEIDQRRLRVSRQSGPDQPVSIGFRDEIIEISAGQAEEFSLARTQ
                     M"
     gene            complement(1594..3528)
                     /gene="treY"
                     /locus_tag="AC150V_00095"
     CDS             complement(1594..3528)
                     /gene="treY"
                     /locus_tag="AC150V_00095"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HIR3761355.1"
                     /translation="MRATYRLQLGPHLSFAEAEALVPYLVDLGVSHLYLSPSFEARSG
                     STHGYDIVDPSRISAERGGEEAFRRLCSAGLDIVLDIVPNHMAAVDENPYWADPPRRQ
                     TFFDIDERSGRHRRFFDIDELAGVRVEDEAVFDGTHRLALELVREGLVDGLRVDHVDG
                     LADPRGYLERLRAEGASRVWVEKILEEGEELRDWQVEGTTGYEFLNDAQRLLVDPEGE
                     ETLTRLYEQVTGEPSAFSDVAARAKLEQARTTFQPEAERLRRLWDAPELERALASLPV
                     YRTYAEPWSGRVEDADRAALAEARLPAELEDVLLLRRPGLGEFVTRFQQTTGAVMAKG
                     VEDTAFYRYGRLLALNEVGGDPGLFHLGVDAFHERNVARAERFPRSLLAGSTHDTKRS
                     ADVRARIGALSGLAEEWTARVRRWLALTEPLTNDGAPDDNERYLLFQTLVGAWPVDSG
                     RIEQYAIKALREAKRNTSWVDQNESWEDATTSFCASLFRHTDFLADLASFAPLVASAG
                     ERAALAELLLRLTCPGVPDIYQGDELPFLALVDPDNRRPVDWERRRRLLAEAKNGAAP
                     SPETAKLHLIARVLDLRSQRPQSFEGPYTPVDAGRNACSYRRGDDVLVWVPLRDGIAK
                     PTQAGLSDLLPEYPAALYVG"
     gene            complement(3525..>3916)
                     /locus_tag="AC150V_00100"
     CDS             complement(3525..>3916)
                     /locus_tag="AC150V_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HIR3761356.1"
                     /translation="RRRWFLSGEQVMGSGLPDVWWFRPDGRKMTQRDWNTPDVHALGV
                     FLNGEEIRRRTHSGERIVDDSFLLLLNAHGEPVEFKLPPRRFGARWMLELSTENGETV
                     SDDAPAHAARTPLTVGARSLVLLRRVR"
BASE COUNT          637 a         1293 c         1361 g          625 t
ORIGIN      
        1 ctgtcctggg gtcatctgtg ggaacagtgc cgactcgaca tcggggccgg ggagcgcccg
       61 gagcagatcc tccagttgca tgttttccat ctgctgcaga cggtctcacc ccacacgatc
      121 gagctcgatg ccggcgttcc ggcgcgcggt ctgcacgggg aggcataccg cggccacatc
      181 ttctgggacg agctcttcat cttccccttc ctcaacctcc ggcttcccgg catcacgcgc
      241 gcactgctcc gctaccgatt tcggcggctg cccgaggcgc gctgggctgc ccagcaggcc
      301 ggcttcgacg gcgcgatgta cccgtggcag agcggaagtg acggtcgcga ggagagccag
      361 acgtggcatg tgaatccggc gtcgggacac tggctcgcgg atcactcgca cctccagcgc
      421 cacatcggcg tcgcggtcgc ctacaacgtc tggctgtact accagacgac gggcgacctc
      481 gacttcctgg cgtactgggg ggccgagatg atgctcgaga tcgcccgctt cttcgcgagc
      541 gcctcgacct acgaccgctc gcgcgaccgc tacgagatca agaacgtgat ggggccggac
      601 gagtaccacg aggcgtatcc gggcgccgcc gagcctgggc tcgacaacaa cgcgtacacg
      661 aacgtcatgg ccgcatgggt cttgacgcgg gcgctcgagg tgctggacct ggtgccggaa
      721 atccggcgcc agcgcctctg ggagaagctc ggcctgaccc gagaggagct cgacaagtgg
      781 gagggcatca gccgcaagat gctgatcccc ttccacgacg agggcatcat cagtcagttc
      841 gaggggtacg gggagctcgc ggagttcgac tgggacggct accgcgagcg gtacggcgac
      901 atctcgcgac tcgaccgcat tctcgaatca gagggcgact ccccgaacaa ctacaagctc
      961 tcgaagcagg cggacgtgct gatgctcttc tacctcctct cgggcgagga gctggcaggg
     1021 gtcttcgagc ggctcgggta cacgttcgac gacaacctga tcccgaggaa cgtcgagtac
     1081 tacacgcgcc gtacgtcgca cggctcgact ctctcgcgtg tcgtcaacgg ctgggtgctt
     1141 tcccgactcg accggcggca gagctgggag ttcttcatgg cggcgctcga gagcgacgtc
     1201 tcggacgtgc agggggggac gacccgggag ggcatccatc tcggcgcgat ggcgggaacg
     1261 gtcgatctcg tccagcgctg tttcggcggt ctcgagacga gagaagacgt gctctggctc
     1321 gatccgcagc tgccgccgga gctcggccgc ctccgcttcg acgtccgcta ccgcaactct
     1381 tggctctcga tcgagatcga ccagcgccgg ctccgcgtga gccgccagtc ggggcccgac
     1441 cagcccgtca gcattggatt ccgtgacgag atcatcgaga tctcggccgg gcaggcggag
     1501 gagttctcgc tggcgaggac tcagatgtga cgaccgcatg aacgaggtgc gtcagcctgc
     1561 taggcggacg agtggcgttg cggcgggacg gcgtcagccg acatagaggg cggccgggta
     1621 ttcgggcagg aggtcgctca gaccagcttg agtcggcttg gcgatcccgt cgcggagcgg
     1681 cacccagacc agcacatcgt cgccgcggcg atacgagcag gcatttcgac cggcatcgac
     1741 gggggtgtag ggaccttcga acgactgcgg tcgctgtgag cggagatcga gaacccgggc
     1801 gatgaggtgg agcttcgccg tctccgggga cggcgcagcg ccgttcttgg cctcggcaag
     1861 cagacggcgt ctccgctccc agtcgaccgg gcggcggttg tccgggtcga cgagtgcaag
     1921 gaacgggagc tcgtctccct ggtagatgtc cgggacgccg ggacaggtga ggcggagcag
     1981 tagctcggcc agcgcggcgc gctccccggc gctcgcgacg aggggcgcga aggatgcgag
     2041 atcggccagg aagtccgtgt ggcggaatag gctggcgcag aagctcgtgg tcgcgtcttc
     2101 ccagctctcg ttctgatcga cccacgacgt gttccgcttc gcctcgcgca gcgccttgat
     2161 cgcgtactgc tcgatccgac ccgaatcgac cggccaggca ccgacaagcg tctggaagag
     2221 caggtaacgc tcgttgtcgt cgggcgcgcc gtcgttggtc agcggctcgg tgagggccag
     2281 ccacctgcga acgcgggcgg tccactcctc ggcgagacca gaaagcgctc ctatgcgcgc
     2341 gcggacgtcg gcgcttcgct tggtgtcgtg tgtcgaccct gcaagcagcg agcgcgggaa
     2401 gcgctcggca cgggcgacgt tccgctcgtg gaacgcgtcg acaccgaggt ggaagagccc
     2461 tggatcaccg ccgacctcgt tcagtgcgag cagccgcccg tagcggtaga aggcggtgtc
     2521 ctcgacccct ttcgccatca cggcgccggt cgtctgctga aaacgagtca cgaactcgcc
     2581 cagccccgga cggcgcagca gtagtacgtc ctcgagctcg gctggaagcc tggcctcggc
     2641 gagggccgcg cgatcggcgt cctcgacgcg accggaccag ggctcggcgt acgtgcggta
     2701 cacgggaagc gacgcgagcg cgcgctccag ctcgggcgcg tcccacaggc gacggaggcg
     2761 ctcggcctcc ggctggaagg tcgttcgcgc ctgctcgagc ttggcgcggg ctgcgacgtc
     2821 cgagaacgcg ctcggctctc ccgtcacctg ctcgtagaga cgtgtcagtg tctcctcgcc
     2881 ctcggggtcg acgaggaggc gctgcgcgtc gttcaagaac tcgtaacctg tcgtcccctc
     2941 gacctgccag tcccgcagct cctctccttc ctcgaggatc ttctccaccc agacgcgcga
     3001 cgcgccttcc gcgcgcaatc gctcgaggta gccgcgcggg tcggcgaggc cgtccacatg
     3061 gtcgacgcgc agcccgtcca caaggccctc gcgcaccagc tcgagggcga gacgatgcgt
     3121 gccgtcgaac accgcttcgt cctcgactcg gacgccggcg agctcgtcga tgtcgaagaa
     3181 ccggcggtgc cgcccactgc gctcgtcgat gtcgaagaag gtctgccgcc gtggcggatc
     3241 cgcccagtac ggattctcgt cgacggccgc catgtggttc gggacgatgt ccaggacgat
     3301 gtcgagccct gccgagcaga gacgacggaa ggcttcctcg ccgccccgct cggcggagat
     3361 gcggctggga tcgacgatgt cgtagccgtg ggtcgagcct gatcgggcct cgaacgaggg
     3421 cgagaggtag aggtggctca ctccgagatc caccaggtag gggacgagtg cctccgcttc
     3481 ggcgaaggag aggtgtgggc cgagctgcaa gcggtaggtc gccctcaccg cacgcgacgc
     3541 aagaggacga gcgagcgcgc gccgaccgtc agtggcgtgc gcgccgcatg ggcgggcgcg
     3601 tcgtccgaca ccgtctctcc gttctcggtg gacagctcga gcatccaccg ggctccgaat
     3661 cgccgcggcg gcagcttgaa ctcgaccggc tcgccgtggg cgttgagcag aaggaggaag
     3721 gagtcgtcga cgatccgctc gccggagtgt gtgcggcgtc ggatctcctc gccgttcaga
     3781 aagacgccga gcgcatgcac gtcaggcgtg ttccagtcac gctgcgtcat ctttcgaccg
     3841 tccggtcgga accaccagac gtcgggcagt cccgagccca tcacctgctc gccggacagg
     3901 aaccagcgac gtcgga
//
LOCUS       DBNGNK010000006         2804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_716929,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000006 DBNGNK010000000
VERSION     DBNGNK010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_716929"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2024
                     /locus_tag="AC150V_00105"
     CDS             <1..2024
                     /locus_tag="AC150V_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012166214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3761357.1"
                     /translation="AWVTLDAPDNDPARLWAHILAALERAGLPVDDALRRRASGADRV
                     AGFLPLLVNALAGTDRRIVLVLDDFHLLEGNEVLDEVEYLIDHLPDQCRLAIGTRIDP
                     PLPLARLRARGELCEVRDDVLRFTDSEATELIAHELGVEIGAKAVGALLVRTEGWAVG
                     LYLAGLGLRGAEDVEAAVAAFSGARRHVVEFLVDEVLADLGPDTRTFLFRSSVLDRMT
                     GDLCDTMLERRGSAELLETLVRSNKLLVPLDDERQWYRYHHLVSDFLRAELARSDAKA
                     IPELQLRASAWYEEAGLLEEATAHAIASGDLDVAGHLIARALIPLERVGRFATITGWL
                     AGFGEDDVAKDTRLAITAAWVALARGDSDEAERWLDVADGYEPDAAYDGWASIGVVAP
                     MTRAASAYVRSAPVEAASEAQCSIDAGPPSLWSTRASVIMGASLYWRGQLGQARAHFE
                     QLLPRLEGESVPVVIALGYLGAIALEEGDAEGARIRCQQALDVAEDSHLGRHLRTAGV
                     QLTMCRLHLLEGDPDAAQRRIATELEPLRQRLDPANHAALLLVEARASLALGDTAAAE
                     ALLARAGDVVAICGEPEVIAARLTEVEPTLARAVAAPRGSVRQREELSDRELDVLRLL
                     PTRLTKREIGRELYISLDTVATHTRSIYRKLGIHSRSAAVERARALRLI"
     gene            2055..2309
                     /locus_tag="AC150V_00110"
     CDS             2055..2309
                     /locus_tag="AC150V_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761358.1"
                     /translation="MKTGHLDLWTLQAMEPSRYRIVARGEFGARLGDMVAGWSVEASE
                     GETVLEGNIRDQAELHGLLARLRSFGVELVSVNPLSDQEG"
     gene            complement(2332..>2804)
                     /locus_tag="AC150V_00115"
     CDS             complement(2332..>2804)
                     /locus_tag="AC150V_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3761359.1"
                     /translation="RPKYPHLEITGSTDLEQVVRDADVIVTGTHARGWIVDEAWVRPG
                     AHLAALGADLAGEQELHPGLLQRGRVFVDDIRQCREDGEINVPLTEGLITEDDVAGEI
                     GKVICGELEGRQSDDQITVFDSTGIALQDSATVPLEYERALASGVGVEKKMIST"
BASE COUNT          411 a          933 c          998 g          462 t
ORIGIN      
        1 tcgcctgggt gacgctcgac gctccggaca atgacccggc ccgtctgtgg gcgcacatac
       61 tcgcggcgct cgagcgcgcc ggtctgccgg tagacgatgc gttgcgtcgc cgggccagcg
      121 gagccgaccg cgtcgccggc ttcctacctc tgctggtgaa cgcgcttgcg ggaacggatc
      181 gacggatcgt cttggtgctc gacgacttcc acctgctcga gggcaacgag gtgctggacg
      241 aggtggagta tctcatcgac catctgccgg atcagtgccg gctcgcgatc ggcacccgca
      301 tcgacccgcc tctgccgctc gcacgcctgc gagcccgagg tgagctatgc gaggtgcgcg
      361 acgacgttct ccgcttcacc gactccgagg ctacggagct gatcgcccac gaactcgggg
      421 tcgagatcgg ggcgaaggcc gtcggcgcgc tccttgtccg taccgagggc tgggcggttg
      481 gcctctacct cgcgggactc ggtctgcgtg gtgccgagga cgtcgaggcc gctgtcgcag
      541 ctttcagcgg agcgcgtcgg cacgtcgtgg agtttctcgt cgacgaggtg ttggctgacc
      601 ttggcccgga cacgcggacg ttcctgttcc gctcgtctgt tctcgaccgg atgacaggag
      661 acctctgcga cacgatgctt gagcgccggg gatcggccga gctcctcgag acgctcgtcc
      721 ggtcaaacaa gcttctcgtg ccgctcgacg acgagcggca gtggtaccgg tatcaccatc
      781 tcgtctccga cttcctccga gccgagctcg cgcgatccga cgcgaaggcg attccggagc
      841 tgcagctccg cgcgagcgcc tggtacgagg aagctgggct cctggaggag gcaaccgctc
      901 atgcaattgc gtcaggcgac ctcgatgtcg ccggccacct gattgcgcgc gcgctgatac
      961 ctctcgagcg ggttggccgc ttcgcgacga tcaccggctg gctggcaggg ttcggcgagg
     1021 acgacgtggc caaggacacg agactggcga tcaccgccgc gtgggtcgcg ctggctcgcg
     1081 gggactccga cgaagcggag cggtggctcg acgtcgccga cggctacgag cccgacgcgg
     1141 cctatgacgg ttgggcgtcg atcggggtgg tggcgccgat gacccgagcg gccagcgcgt
     1201 acgtgcggag cgcgcccgtc gaagcggcct ccgaggccca gtgctcgatc gacgcggggc
     1261 cgccgtcgct gtggagcacc cgcgcctcgg tgatcatggg agcgagcctc tactggcgcg
     1321 gccagctcgg gcaggcccgg gcgcacttcg agcagctgct tccccggctg gagggcgaga
     1381 gcgtccccgt cgtaatcgcc ctcggctacc tgggtgcgat cgcgctcgag gagggggacg
     1441 ccgagggcgc ccggatccgg tgccaacagg ccttagacgt cgccgaggat tcccatctcg
     1501 gtcgacacct caggacggcg ggcgtgcagc tgacgatgtg caggctgcac ctactcgagg
     1561 gcgacccgga cgcggcgcaa cggcgtatcg ctaccgagct ggagccgctg cggcagcggc
     1621 tcgatccggc caaccacgcg gcactcctac tcgtcgaggc gcgggcgagt ctggcgctcg
     1681 gtgacactgc ggcggcggag gccctcctcg ctcgggctgg agacgtggtg gcgatttgcg
     1741 gtgagcccga ggtgatcgcg gcaaggctca cggaggtcga gccgacgctc gcacgtgcgg
     1801 tcgcggcgcc ccgcgggagc gtccggcagc gcgaggagct cagcgaccgg gagctcgatg
     1861 tcctcaggtt gcttccgaca cgcctcacca agcgcgagat cggccgcgag ctctacatca
     1921 gcctcgacac cgtcgcaaca catacgcgat cgatctaccg taagctcggc atccattcgc
     1981 gctcggcggc tgtggaaaga gctcgagcgc tcaggctgat ctgagcagct cacccgggtg
     2041 aacgcgcaga tcgggtgaag accggtcacc tggacctgtg gacactccag gcgatggagc
     2101 cctcccgcta caggatcgtg gcgcgcggcg agttcggtgc gaggctcggc gacatggtcg
     2161 cggggtggag cgtcgaagcg tccgaggggg agacggttct cgaggggaac atccgcgacc
     2221 aggccgagct gcacgggctc ctcgcacggc tcaggtcgtt cggcgtcgag ctcgtgagcg
     2281 tgaatcccct ttccgatcaa gaggggtgac tgttggtgct tgggctgacg cctaggtgct
     2341 gatcatcttc ttctcgacgc cgaccccgga cgcaagcgcc cgctcgtatt cgagcggaac
     2401 cgtcgcggag tcctgcaacg cgatgccggt cgagtcgaag accgtgatct ggtcgtcgga
     2461 ctgacgccct tcgagctcgc cgcagatgac cttgccgatc tcgccagcga cgtcgtcctc
     2521 ggtgatcagg ccctcggtga gcggcacgtt gatctcgccg tcctcgcggc actgccggat
     2581 gtcgtcgacg aagacgcgtc cacgctgtag caggcccggg tgcagttcct gctcgcccgc
     2641 caggtcggcg ccgagcgcgg cgaggtgtgc tccgggcctc acccacgcct cgtcgacgat
     2701 ccaaccgcgg gcgtgggtgc cggtgacgat cacgtcggcg tcccgtacga cctgctcgag
     2761 gtcggtcgac cccgtgatct cgaggtgcgg gtacttcggg cgct
//
LOCUS       DBNGNK010000007         4612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_573704,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000007 DBNGNK010000000
VERSION     DBNGNK010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_573704"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..2451
                     /locus_tag="AC150V_00120"
     CDS             244..2451
                     /locus_tag="AC150V_00120"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR3761360.1"
                     /translation="MTVVDATRPHESLVNELIASVEAYNPSVDRELLRRAFDYAEGHH
                     RRQRRRSGEEFIHHPYGVAQICAELHLDDPTIAAALLHDVIEDTDADPEEVRNEFGAE
                     IATLVDGVTKLTRITFQSREQAEAENYRKMIVAMAQDVRVILIKLADRLHNMRTIEYL
                     GKQKQVQKATETLEVYAPLAHRLGIHALKWELEDLSFQILHPRKYAEIKAMVADRRAD
                     RESQVEAAGELLWAELEKVDIPAEILGRAKHFYSIYDKMSKKGREFNEIYDLTAMRVI
                     VERSGEAGTRDCYGALGLIHSLWKPMPGRFKDFVAMPKLNRYRSLHTTVIGPEGTPLE
                     IQVRTREMHEEAEYGVAAHWLYKHDKKSSKADDEWLAWVKTLMDWQGEESDPSEYMRT
                     LRTDLFHDEVYVFTPKGEVKTLPAGATPIDFAYAVHTDVGHRTVGAKVNGRIVPLHYR
                     LKNGDFVEILTSKSGRGPSRDWLSLAASSRARNKIRQWFQRETKGETEQKGRDLLDQA
                     LKAQNLPYRKLNSSSVLAQVIRETGFKKAEDFYIALGSGKLGTGQIVNKVLQRLKTDE
                     VAQEPLVAQKPAKARTAVTGETVGIAVPGVPDVLVRLAKCCTPVPGDEIVGYISLGRG
                     ITIHREDCPNVRSLMRNPERFTPVEWDGDQSTSFRVQIAVEAWDRARLLEDVARTFAE
                     YGANIVSYGGSVDDGLARNWYTAELGDTKSLRSLLTALRNLDAVFDAYRVTPN"
     gene            2511..3185
                     /locus_tag="AC150V_00125"
     CDS             2511..3185
                     /locus_tag="AC150V_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3761361.1"
                     /translation="MQEILVVDDESAVREAIERALRLEGFDVSLASDGEQALTSIAET
                     RPDAVVLDVLMPGLDGITACRRLRAGGDRTPVLVLTARDAVSDRVAGLDAGADDYLVK
                     PFALEELLARLRALLRRTDGGTGGALQFADLSLNPSTREVTRGERGIELTRTEFLLLE
                     LFLVNPRQVLPRTLIFERVWGYDFGPSSNTLEVYIGYLRRKLEADGESRLIHTVRGVG
                     YVLREE"
     gene            3182..4516
                     /locus_tag="AC150V_00130"
     CDS             3182..4516
                     /locus_tag="AC150V_00130"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3761362.1"
                     /translation="MSFRARLALAAALAVAAAVVAASAAAYVIVRDELRDGIDQSLRE
                     RVDQSLRPVFQGRRPGRPFDPVPPPRFGGAPGFAQLVGPGGEVALARGEETALPVSDG
                     ALAVARGEAQERFEDIKVDDLNLRVLTVGLGEVGAIQLARPLDEVDDVLGRMRTALLL
                     VGAGGVALAAVLGLVVSGAAIGPLRRLTAAAEDVTRTRDLTQRVGVSGDDEIGRLGVR
                     FDEMLAALEQSRQAQRQLVADASHELRTPLTSLKTNIEVLAGDKQLADTERRRMLNDV
                     VLQLDEMTALVSDVVELARDGEREEELGDVRLDLVVAAALERVRRRAPNTTFESEIEE
                     SVVLGVATRIDRAVTNLLDNAVTWNAVGQPIEVSVSDARFSVRDHGAGIDEDDLPHVF
                     DRFYRSAAARGKPGSGLGLAIVRQVADAHGGRAWAERAAGGGTRVSIEFVSA"
BASE COUNT          790 a         1519 c         1601 g          702 t
ORIGIN      
        1 acgcgctgcc cgaggcagcc tgaggctgag cgggctcgtt caggggcgcg cgggaacgcg
       61 ccggcgcagc agcgccggct ggcgcggtgg ccgcgcggcg ccaccggcag ctcggtcgag
      121 ctcgagaatg aggcgccgca cgctgcggcg ctcgcggatg cggcggaaga ccatcgagct
      181 gtcaggttcg cgcccgttcg cgccgatcct ttcccgaaag agtcgcgaag tacactgaat
      241 gcaatgacgg tcgtcgatgc cacccgccca cacgagagcc tcgtcaacga gctgatcgcg
      301 agcgtcgagg cctacaaccc gagcgtcgat cgcgagctcc tccgccgggc ctttgactac
      361 gcggagggac accaccggcg ccagaggcga cgctccggcg aggagttcat ccatcacccc
      421 tacggcgtgg ctcagatctg cgccgagctt cacctcgacg atccgaccat cgcagcagca
      481 ctgctacacg acgtgatcga ggacaccgac gccgatccgg aagaggtcag gaacgagttc
      541 ggtgccgaga tcgcgacgct cgtcgacggt gtcaccaagc tgacgcgcat caccttccag
      601 agccgcgagc aggccgaggc ggagaactac cggaagatga tcgtcgcgat ggcgcaggac
      661 gtccgcgtca tcctgatcaa gcttgccgat cggctccaca acatgcgcac gatcgagtat
      721 ctcggcaaac agaagcaggt gcagaaggcg accgagacgc tcgaggtcta cgctccgctc
      781 gcacaccgac tcggaatcca cgctctcaag tgggagctcg aggatctctc cttccagatc
      841 ctccatccac gcaagtacgc cgagatcaag gcgatggttg ccgatcgtcg cgccgaccgc
      901 gagtcacagg tcgaggcggc cggcgaactt ctttgggccg agctcgagaa ggtcgacatc
      961 ccagcggaga tcctgggccg cgccaagcac ttctactcca tctacgacaa gatgtccaag
     1021 aagggccggg agttcaacga gatctacgac ctcacggcga tgcgcgtcat cgtcgagcgc
     1081 tccggcgagg cgggcacgcg agactgctac ggcgcgcttg gcctgattca ctcgctctgg
     1141 aagccgatgc ccggccggtt caaggacttc gtggcgatgc cgaagctgaa ccggtaccgc
     1201 tcgctgcaca cgacggtgat cgggcccgaa gggacgcctc tcgagatcca ggtgcggaca
     1261 cgagagatgc acgaggaggc cgagtacggc gttgccgcgc actggctcta caagcacgac
     1321 aagaagagct cgaaggcaga cgacgagtgg ctggcatggg tgaagacgct catggactgg
     1381 cagggcgagg agtccgatcc ctccgagtac atgcggacgc tccgcaccga tctcttccac
     1441 gacgaggtct acgtcttcac gccgaagggc gaggtgaaga cgctccccgc cggcgcgacg
     1501 ccgatcgact tcgcgtacgc ggtgcatacc gacgtcggcc acaggaccgt cggcgccaag
     1561 gtcaacggcc gcatcgtgcc gctgcactac cgcctcaaga acggggattt cgtagagatc
     1621 ctcacttcga agtccggccg cgggccgtcg cgtgactggc tctcgcttgc ggcctcctcg
     1681 cgggcacgca acaagatcag gcagtggttc cagcgcgaga ccaagggcga gaccgagcaa
     1741 aagggtcgtg acctgctcga ccaggcgctg aaggcacaga acctccccta ccgcaagctg
     1801 aacagctcga gcgtgctcgc acaggtgatt cgcgagaccg gcttcaagaa ggcggaagac
     1861 ttctacatcg ctctcggctc gggcaagctc ggaacgggcc agatcgtcaa caaggtgctc
     1921 cagcggctga agacggacga ggtcgcgcag gagccgctcg tcgcccagaa gcctgcgaag
     1981 gcgcgaaccg ccgtgacggg cgagaccgtg ggcatcgcag tgccaggcgt tcccgacgtg
     2041 ctcgtgcgcc ttgcgaagtg ctgcacaccc gtgcccgggg acgagatcgt cggctatatc
     2101 tcgctcggcc gcgggatcac cattcaccgc gaggactgcc cgaacgtgcg gtcgctgatg
     2161 cggaacccgg agcggttcac gccggtcgag tgggacggcg accagtccac gagcttccgc
     2221 gtccagatcg ctgtggaggc ctgggaccgg gctcgcctgc tcgaggacgt ggcccgcacc
     2281 ttcgccgagt acggcgccaa catcgtcagc tacggcggct cggtcgacga cgggctggca
     2341 cgcaactggt acacggccga gctcggcgac acgaagagcc tccgctcgtt gctgacggcc
     2401 ctgcgaaacc tggacgccgt cttcgacgca taccgcgtga cacccaacta gccggactcc
     2461 ccggaggggc ctctcaggaa actttcagca gctccgggca tgctttgcga atgcaggaga
     2521 tcctcgtagt cgacgacgag tccgcggttc gagaggcgat cgagcgcgcg ctgcgcctcg
     2581 agggcttcga tgtctccctc gcatccgacg gggaacaagc gctcacgagc atcgccgaga
     2641 ctcgacccga cgcagtcgtg ctcgacgtct tgatgcccgg gctggacggg atcacggcct
     2701 gccgtcggct ccgggccggc ggagaccgca cgccggtgct cgtcctcacc gcccgcgacg
     2761 cggtctccga ccgggtggcg ggtctcgatg cgggggcgga cgactacctg gtcaagccgt
     2821 tcgcgctcga ggagctgctc gcgcggctcc gcgcactgct gcggcgcacg gacggcggaa
     2881 cgggcggcgc actgcagttc gccgacctct cgctcaatcc gtcgacgcgg gaggtgaccc
     2941 gcggcgagcg tggcatcgag ctgacgcgga ctgagttcct gctactggag ctgttcctcg
     3001 tgaacccgcg gcaggtgctg ccgcgtacgc tcatcttcga gcgcgtgtgg ggctatgact
     3061 tcgggccgtc gtcgaacacg ctcgaggtgt acatcgggta ccttcgccgt aagctcgaag
     3121 cggacgggga gagtcggctg atccacacgg tgcgcggcgt gggttacgtc ctgcgcgagg
     3181 aatgagcttc cgcgcgcgcc tcgctctggc cgcggcgctc gccgtagcgg ctgccgtggt
     3241 ggccgcgtcg gccgcggcgt acgtgatcgt ccgcgacgag ctgcgcgatg ggatcgacca
     3301 gtccctgcgc gagcgagtcg atcagtccct gcggccggtg ttccagggac ggcgcccggg
     3361 aagacccttc gatccggttc cgccgccgag gttcggcggc gcgcccggat tcgcgcagct
     3421 cgtcgggccc ggcggggaag ttgccctggc gcgaggcgag gagacggcgc tgccggtgag
     3481 cgacggggcc ctcgccgtgg cccgcggcga ggcgcaggag cgtttcgagg acatcaaggt
     3541 cgacgatcta aaccttcgcg tcctcacggt ggggctcggc gaggtgggcg cgatccagct
     3601 cgcacgaccg ctcgacgagg tcgacgatgt gctcggccgg atgcgaaccg ctctgctcct
     3661 ggtgggcgcg ggcggtgttg ccctagccgc cgtgctcggt ctcgtcgtca gcggagcggc
     3721 gatcgggccg ctgcggcgct tgaccgccgc tgccgaggat gtgacgcgca cgcgcgacct
     3781 gacgcagcgt gtgggtgtct cgggggacga cgagatcggg cggcttggcg tgcggttcga
     3841 cgagatgctc gcggccctcg agcagtctcg ccaggcacag cgacaactgg tcgccgatgc
     3901 ctcccacgag ctgcggacgc cgttgacgag cttgaagacg aacatcgaag tgctcgcggg
     3961 cgacaagcag cttgccgaca cagagcggcg gcgcatgctc aacgacgtcg tgttgcagct
     4021 cgacgagatg acagctctgg tgtcggacgt cgtcgagctc gcccgggacg gcgagcgcga
     4081 agaggagctc ggcgacgtcc ggctcgatct cgtggtcgcg gccgctctcg agcgcgttcg
     4141 ccggcgcgcg ccgaacacga ccttcgagag cgagatcgag gagtccgtcg ttctcggtgt
     4201 cgccacccgg atcgatcggg ccgtgacgaa tttgctcgac aacgccgtca cgtggaacgc
     4261 ggtcgggcag cccatcgaag tctcggtcag cgatgcgcgc ttcagcgtgc gagatcacgg
     4321 cgcagggatc gacgaggacg acctgccgca cgtcttcgat cgcttctacc ggtcggccgc
     4381 cgcccgaggc aaacccggtt ccgggctcgg tcttgcgatc gtccggcagg tggcggacgc
     4441 ccacggtggt cgcgcctggg ccgagcgcgc cgcagggggc ggaacgcgcg tctcgatcga
     4501 gttcgtttcc gcttgaacaa gccgttttcg gcgcctctcc ctcgaacgag ggattgtgtc
     4561 cctcgggagg gttattacaa ggacataggg gagggggaag ccagtcgcag cg
//
LOCUS       DBNGNK010000008         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_573826,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000008 DBNGNK010000000
VERSION     DBNGNK010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_573826"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..413)
                     /locus_tag="AC150V_00135"
     CDS             complement(12..413)
                     /locus_tag="AC150V_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR3761363.1"
                     /translation="MSGKPKVTTHDEYLAALGDDKRAALESLSGVIRSVAPGVEECFS
                     YGLPAFRLDGQVLVLFGATADHCSFYPGSGTAVEAHEDELEGYSTSKGTIRFDPDQPL
                     PARLVRTLVEYRIAENAARRPHTRAGAPRRR"
     gene            complement(479..784)
                     /locus_tag="AC150V_00140"
     CDS             complement(479..784)
                     /locus_tag="AC150V_00140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3761364.1"
                     /translation="MIVVRFKVTCKPEKALQVRAALEAVVAPSRELAGVHSFDVAQDL
                     VDPHSFIATEVFADRDALARQEALPEVQNVLTLLPESLAAEPEATVYEASASRPWGA"
     gene            complement(825..2090)
                     /locus_tag="AC150V_00145"
     CDS             complement(825..2090)
                     /locus_tag="AC150V_00145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3761365.1"
                     /translation="MNRDALDRLRRDPIDWRYKGFPPAEGVTPAAVGRQGWSLLRGDL
                     PTPVMVLRESALAHNLELMARWCRERDVSLAPHGKTTMSPELYERQLAAGAWGITVAN
                     AAQARVCRAFGVERILLANELVEPASIRFVTAELAADPGFDFYCLADSVAAVDALAGA
                     AADTLRPIQVLVELGLAGGRTGSRTAAEAREVAAAIVAAPALELAGVEGYEGILHGDD
                     SLPAIDAFLDALRSLVTVLAADGAFAERSEAVVTAGGSAYFDRVAERLGQPVGELQVR
                     IVLRSGCYLTHDSAFYEHLSPLGGRSSGGPRLRPALEAWGAVLSRPEPDLALLLLGKR
                     DVPYDIDLPIPLHVASDGALRDVRGTMTVFDTNDQHAYLRVPDDDPLSVGDLVGCGIS
                     HPCTAFDKWRLIPVVDDEYAVVDAVHTFF"
     gene            2115..>2424
                     /locus_tag="AC150V_00150"
     CDS             2115..>2424
                     /locus_tag="AC150V_00150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761366.1"
                     /translation="MRFADRTIVVTGGGSGIGRVMAKRFAAEGGRVVIADLLADKAAE
                     VAAEIAADGGQSVSLRANVTLTGDVESMVQAAEDAFGPVDVLVNNAYSCAGDNVVLID
                     D"
BASE COUNT          368 a          821 c          865 g          370 t
ORIGIN      
        1 gacgcccagc atcagcggcg gcgcggagcg ccagcccgtg tgtgcggtcg tcgggccgcg
       61 ttctccgcga tccggtactc caccaacgtc cgcaccagtc gtgcgggcag cggctggtcc
      121 ggatcaaacc gaatcgttcc cttgctcgtg ctgtacccct cgagctcatc ctcgtgagct
      181 tccacagcag tgccgctccc cggatagaac gagcagtgat cggccgtcgc gccgaagagc
      241 actagtacct gcccgtcaag ccggaatgcc ggaagcccat aactgaagca ctcctcgacc
      301 ccgggagcca ccgaccggat gactccgctc aggctctcga gtgcggctct cttgtcatcg
      361 cccagggcgg ccaggtactc gtcgtgggtc gtgaccttgg gtttcccgct cacgagttgt
      421 gctccctcaa aggcggactg ctcgggcgct cctcgaccgg ccgcttcgac ctcggctctc
      481 aggctcccca cggccgcgac gccgaggcct cgtagacggt cgcctctggc tctgctgcga
      541 gcgactccgg gagcagcgtg agcacgttct gaacctcggg gagtgcctcc tgacgcgcga
      601 gcgcgtcacg atcggcgaag acctccgtgg cgatgaacga gtggggatcc actaggtctt
      661 gggcgacgtc gaacgagtgt acccctgcga gctctcggct aggcgcaacg accgcctcga
      721 gggcggcgcg tacctgaagg gctttctcgg gcttgcaggt gaccttgaag cgcacgacga
      781 tcatgtcctc tcctttcgcg tcgtcgacgg cgacggctcc ggcgtcagaa gaacgtgtgc
      841 acggcgtcga cgaccgcgta ctcgtcgtcg acgaccggaa tcagccgcca cttgtcgaac
      901 gccgtacagg gatgcgagat cccgcagccg acaaggtcgc ccacggacag gggatcgtcg
      961 tcgggaactc gtaggtacgc gtgctggtcg ttcgtgtcga acacggtcat cgtcccgcgg
     1021 acgtcgcgga gcgctccgtc cgaagcgacg tggaggggga tggggaggtc gatgtcgtac
     1081 gggacgtcgc gcttgccgag gagcaggagg gcgaggtctg gctcggggcg cgagagcacc
     1141 gcaccccagg cttcgagcgc gggccggaga cggggtccac ccgagctgcg accgccgagc
     1201 ggagaaaggt gctcgtagaa ggcggagtcg tgggtgaggt agcagccgct ccgcaggacg
     1261 atgcgaacct ggagctcgcc gacaggctgc ccgagtcgct cggcgacccg gtcgaagtag
     1321 gcgctgccgc cggccgtcac gaccgcctct gaccgttctg cgaaggcccc gtccgcggcg
     1381 agcacggtca cgaggcttcg gagggcgtcg aggaaggcgt cgatcgccgg cagcgagtcg
     1441 tcgccgtgga ggatgccctc gtatccctcg accccggcca gctccagcgc tggagcggcc
     1501 acgatcgccg cggcgacctc cctcgcctcc gcggccgttc gggagcccgt cctcccgccc
     1561 gctagaccca gctcgacgag cacctggatc ggccggagcg tgtcggcggc ggctccggcc
     1621 agtgcatcca cagccgccac cgagtcggcc aggcagtaga agtcgaagcc aggatcggcg
     1681 gccagctcgg cggtaacgaa gcggatcgag gcgggctcga ccagctcgtt ggcgagcagg
     1741 atgcgctcga cgccgaacgc gcggcagacc cgcgcctggg ccgcgttggc gaccgtgatc
     1801 ccccaggcgc cggctgcgag ctggcgctcg tagagctcgg ggctcatcgt cgtcttgccg
     1861 tggggggcga gcgacacgtc gcgctcgcgg caccagcggg ccatcagctc gaggttgtgg
     1921 gcgagcgcgc tctccctgag caccatgacc ggggtgggga ggtcgccgcg gagcaggctc
     1981 cagccctgcc ggcccaccgc ggccggggtt accccttcgg cgggtgggaa ccccttgtag
     2041 cgccagtcga tcgggtcgcg tcggagtcgg tcgagggcat cgcggttcac gccgggattc
     2101 taagatccct ggggatgcgc ttcgcggacc gcacgatcgt cgtcaccgga ggcggctccg
     2161 gaatcggccg cgtgatggcg aagcgcttcg cggccgaggg cggtcgggtc gtcatcgccg
     2221 acctgctcgc ggacaaggcc gccgaggtcg cggccgagat tgcggccgac ggaggtcagt
     2281 ccgtatcgct gcgggcgaac gtcacgctca ccggggatgt cgagtcgatg gtgcaggcgg
     2341 ccgaggacgc cttcggcccg gtcgacgtcc tggtcaacaa cgcgtactcc tgtgccgggg
     2401 acaacgtcgt tctcatcgac gatg
//
LOCUS       DBNGNK010000009         9604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_144047,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000009 DBNGNK010000000
VERSION     DBNGNK010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9604
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_144047"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="AC150V_00155"
     CDS             <1..595
                     /locus_tag="AC150V_00155"
                     /inference="COORDINATES: protein motif:HMM:NF022818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HIR3761367.1"
                     /translation="TLSFEVSGKLRNANLVMIDRQTETWWQQFGGTAIVGRLTGSNLR
                     RLTARLVSWGDFRRQRPTAPVLLRPEGTPYPYGVNPYAGSDTPTSLPLHTYANPGDRR
                     LPPKTRVIYVERRGERVAVPLATLQKKRKLTVTVGGKALVIRKTGEVASPLSDPGIHR
                     GAPIPAISVLEAGRPVPFDTPFWFAVAAFAPDVRLVE"
     gene            681..1088
                     /locus_tag="AC150V_00160"
     CDS             681..1088
                     /locus_tag="AC150V_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3761368.1"
                     /translation="MPTWVLPTLGYGVLLGAAGVTSKLALETIDWRQLVLWVPIAYAV
                     FAVIFIVSNGTTLPLGRGGFWAATTGVFAAASLILLFVALSRGEASIVVPIGAAYPVV
                     TLAGSALFLGENITLPKVIGVALVIAGVVVISR"
     gene            complement(1104..2054)
                     /locus_tag="AC150V_00165"
     CDS             complement(1104..2054)
                     /locus_tag="AC150V_00165"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3761369.1"
                     /translation="MASILRETMWRQPADLRALIEDAAPAEAVAERLAGRRVVLVGTG
                     TSWHAANHGAWLLRVAGVEALPVQAMDEALWGPSARSGDALVVMSHRNTKRFASDVLA
                     HARANGVPTVVISGKGSPGADLETVEPERSPTFTASHLGALLRLAQLARALGARLPEL
                     ERVPEAVEAALSGPGPRVEPPERLLEFTGAGPNQWTAAEGALKVREAARVVTEGLSVE
                     QLLHGPSVVLAEGCALVCLDGGGPAVDRLEEVASAAEDTGVTVYRVVERELGEALSVF
                     PLTVAVQRVALELAETLGTDPDAFGYDVPGRKEVWTRITL"
     gene            complement(2055..3353)
                     /locus_tag="AC150V_00170"
     CDS             complement(2055..3353)
                     /locus_tag="AC150V_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3761370.1"
                     /translation="MSLQEKSAAQSLIEEREHYVSRAVPTPRLVVSRAEGARITDADG
                     HEFLDFVGGIGCQNLGHGHAAIVEAIHDQVDRFLHQMVAVGSYEPYVDVCRRLAELSP
                     CPGPQKSVLVNSGAEAVENAVKVARAYTKRPAVLVFDYAFHGRTLLCMTMTSKLVYKK
                     GFGPFAPEVYRAPAPYPYRGVSSDDALAGIERLFKSDLDPATVACAVLEPVQGEGGFI
                     PMTDDFPARLQELLAGHGILYVDDEVQSGCGRTGRMWAIEHYGVEPDLLISGKTLGGG
                     LPLAAVTGRAEIMDAPDVGGLGGTFGGNPVSCAAAAVVLDQLSQPEPLARGTALGERL
                     RGALDAMQARFEEIGDVRGLGPMLAIELVTDHGTKAPASDLTARTISEAYDRGLLLLA
                     AGLYGNVIRFLPPLNLSDDDTERGLAILDESLAAAGATSR"
     gene            3515..4276
                     /locus_tag="AC150V_00175"
     CDS             3515..4276
                     /locus_tag="AC150V_00175"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HIR3761371.1"
                     /translation="MRTIDINADCGESFGNWTMGNDEALIPLITSANVACGYHASDPL
                     TMRATVRACVANGVAVGSHPGFPDLLGFGRRVMQISPEDAYAYVVYQTGALQAFLAAE
                     GATLHHVKAHGAFYTILREDDDLAAAFCEAVEAVMPEPLVYWPDPTDCSLPRAARQRG
                     LRVVLEIYPDLDYSPEGMLVLQRAKHHRDVAQAAGQVRRWIETGEVEALDGSTIPVDG
                     ESICVHGDGPNVTEITTAIRVVIEESGNQVGQPVI"
     gene            4281..5639
                     /locus_tag="AC150V_00180"
     CDS             4281..5639
                     /locus_tag="AC150V_00180"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3761372.1"
                     /translation="MGFSKVLIANRGEIAVRIIRTLREMGIGSVAVYSEADAEAPHVR
                     WADEAVAIGPGPVGKSYLDADKIIAAARETGAGAVHPGYGFFAEHPEFAAACGDAGLV
                     WVGPPPQAIEAMGLKTRARRLMEEAGVPVVPGTITPAHSPAEALATARDIGFPVAFKT
                     AGGGGGKGFHVAYSEADAASAFERASTEGERFFANPDVYVEEYLEDPRHVEIQVLADT
                     QGNVVHLFERDCSVQRRHQKLIEEAPAPTIDEDTRTRICAIAVDAARSVGYVSAGTVE
                     GLLVGDRFYFLEMNTRLQVEHPVTELLTGIDIVREQIRVAAGEPLSFAQEDIVPRGAA
                     IECRVNAEAAHLDFLPGPGTITAYHEPGGPGVRVDSGIRAGYSVPPFYDSLLAKVVVW
                     DWSREAATARMLRALAEYEIEGLATLLPFHRSFLATRQWRELETGRDLLGDPEWLAAT
                     AP"
     gene            5692..6606
                     /locus_tag="AC150V_00185"
     CDS             5692..6606
                     /locus_tag="AC150V_00185"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004216093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit B family protein"
                     /protein_id="HIR3761373.1"
                     /translation="MPDTGSAKLVEPPLPDPRYSFGGDEFIYCEISEEMSFHANFKGL
                     AICLELQQRALRGVVEICPSNASYQVRIDPDVVAPREMLALLEELYESVDAEHAHFKS
                     RVIDVPAYYNDPWTREVLMNFRDRHQNPTGTDLEYVTKVNGFASEDEFIEAHHRAPYY
                     VSMVGFVPGLPWCFQMVPRERMLEVPKYIRPRTDTPALALSHGGAFAAIYPVRGPGGY
                     QLFAICPVPIFDPKQELPDFQESFVVFRTGDIIKIRPIDREEYDAIRAEVEAKTYRYR
                     MVDVDFEPGAFFDDPDAYNARLLEELYR"
     gene            6603..7577
                     /locus_tag="AC150V_00190"
     CDS             6603..7577
                     /locus_tag="AC150V_00190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017755083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit C family protein"
                     /protein_id="HIR3761374.1"
                     /translation="MIRVREGGVSTTVQDAGRFGLYHLGMPPSGALDGYSYRVANLLV
                     GNDEGAAVLEATFNGPTLEFEHDAVVAVTGADLPPLLEGSEMPQWTAFAVQAGQTLSF
                     DYLRAGARAYIAAAGGFDVPVVFGSRSTYALCAVGGVDGRPIHEGDTLPVGDAPESPS
                     LEGRTVAPGLRPEFAREWEIRVIMGLCDYRLTEEGRRAFLESEWVVTPAADRIGYRYK
                     GPELEFVEREQPFGAGSDPSNVVDAPYPVGSIQVPGGVEPIVLLNDAVTGGGYVTIGT
                     VISADFVRLAHAKTHDKTHFRGVDLDEALSARAEGRARLDEVRARLGA"
     gene            7601..7849
                     /locus_tag="AC150V_00195"
     CDS             7601..7849
                     /locus_tag="AC150V_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HIR3761375.1"
                     /translation="MSEQKTIRSPLPGTFYRRPNPDAEPFVSDGDAVEPGDVVGLVEV
                     MKTFHEVKAADAGVVERFLVENEDPVDAGQEIVALRDA"
     gene            complement(7873..>9604)
                     /locus_tag="AC150V_00200"
     CDS             complement(7873..>9604)
                     /locus_tag="AC150V_00200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3761376.1"
                     /translation="WGAAGGLTVWSSGQMPFLTRWMLAGLLGVREGDVRVLTPHVGGG
                     FGLKVHLYPEEVAVVLAARRLGSPVKWVEDRLEALLASGHSKEVVVELEAAVAADGRF
                     LALTGHYVGDGGAYQGHPWSSLIDPLCAAVLLPSFYRLDAVRYEVDAAMTNKCQSVAY
                     RGVGWTPGHTAREALVDDIARELAIDPLELRLRNAIPDGEPFTTLTDCHFDGGSFSES
                     MRVAADAVGYEAFREEQGRLREQGRYVGVGFSPFVEQGAWAAEIARAMGFPGFHYTDA
                     VTVTMETDGTVTVASGFQSNGQGHETTMAQLAADALGVRFEDVRVVQGDTAATAYATG
                     SWGSRTAVVASGALMLAAEDVRTKLLAVASDLLESSADDLEIAGGVISAKGAPFRSKT
                     MAEVAAAAYWGPRPEGVDPIPAATRSYDPPETYSNACIVAVVEVDPETGVVDVQRIVC
                     VEDCGTVLNPMIVDGQLAGAIAQGVGAVLYEGLPYGEDGQFLAATLADFLYPTATEVP
                     APEIHHLETPSPVTAGGFKGMGEGGLIGAPAAIVNAIADALAPFGAEVEKTPLRPEDV
                     LDLIDCGRGA"
BASE COUNT         1454 a         3338 c         3351 g         1461 t
ORIGIN      
        1 gacgctctcg ttcgaggtga gcgggaagct ccgcaacgcg aacctcgtga tgatcgaccg
       61 ccagacggag acctggtggc agcagttcgg tgggacggcg atcgttgggc gtctgaccgg
      121 ctcgaacctc cggcgcctca ccgcgcgcct cgtctcgtgg ggcgattttc ggcggcagcg
      181 gcccactgcg ccggtcctcc tccgccccga ggggacgccg tacccgtacg gcgtcaaccc
      241 ctacgcgggc agcgacaccc cgacctcgct gccgctccat acgtatgcga atcccggcga
      301 ccgccgactg ccgccgaaga cgcgggtgat ctacgtcgag cgccgcggcg agcgggtggc
      361 cgtgccgctc gcgaccctgc agaagaagcg gaagctgacc gtgacagttg gaggcaaggc
      421 cctcgtgatc cgcaagacgg gcgaagtcgc atcgcctctc tcggatccgg gcatccatcg
      481 aggcgcgccg atccccgcca tcagcgtgct ggaagccggg cgaccggtgc ccttcgacac
      541 accgttctgg ttcgcggtcg cagcgttcgc gcccgacgtg cggctcgtcg agtagcggcc
      601 gccggacgcg gttgcctcgc ttccgcgccg gcgtcaggat cggccgacgc gacaaaaact
      661 cgatgaaagg acggccgacc atgccaacgt gggttctgcc gacgctcggc tacggggttc
      721 tcctcggcgc agccggggtc acctcgaagc tcgcgctcga gacgatcgac tggcggcagc
      781 tggtgctctg ggtgccgatc gcgtacgcgg tcttcgccgt catcttcatc gtcagcaacg
      841 gaacgacgct gccgctggga cgcggcggtt tctgggctgc gacgaccggc gtcttcgccg
      901 ctgcctcgct gatcctcctg ttcgtcgcgc tctcgcgcgg cgaggccagc atcgtcgtgc
      961 cgatcggcgc cgcctatccc gtggtcacgc tcgccggctc ggccctcttc ctgggggaga
     1021 acatcacgct gccgaaggtg atcggcgtcg ccctcgtcat cgccggcgtc gtcgtcatca
     1081 gccggtgaca aaggcggtcg agatcagagt gtgatccggg tccagacctc cttgcggccc
     1141 ggaacgtcgt agccgaacgc gtcggggtcg gtgccgagcg tctccgcaag ctcgagtgcg
     1201 acccgttgca cggcgaccgt gagcgggaag accgagagcg cctcgccgag ctctcgctcg
     1261 acgactcggt acaccgtcac tcctgtgtcc tccgctgccg aggcgacctc ctccagccgg
     1321 tcgacggccg gcccgcctcc gtcgagacag accaacgcgc agccctccgc gaggacgacc
     1381 gacggcccat gcaggagctg ctcgaccgac agcccctcgg tgacgacccg ggctgcttcg
     1441 cggaccttca gcgccccttc ggcggccgtc cactggttcg ggcccgcgcc ggtgaactcg
     1501 agcagtcgct cgggcggctc gacacggggg ccgggccccg agagggcggc ctcgacggcc
     1561 tcgggcacgc gctcgagctc cggcagccgc gcgccgagcg cccgcgcgag ctgcgcaagc
     1621 cggagcagcg cgcccaggtg gctcgcggtg aacgtcgggc tgcgctcggg ctcgacggtc
     1681 tcgaggtccg cgccgggcga acccttcccc gagatcacga cggtcggcac gccgttcgcc
     1741 ctcgcgtgcg cgagaacgtc cgaggcgaag cgcttcgtgt tgcggtgact catgacgacg
     1801 agcgcgtcgc cggatcgggc gctcggtccc cagagggcct catccatcgc ctgcacgggg
     1861 agcgcctcga ccccggccac gcgcagcagc cacgcgccat ggttcgccgc gtgccagctc
     1921 gtgccggtgc cgaccaggac gacgcgccgg cccgcaagtc gctctgccac cgcttcggcg
     1981 ggtgcggcgt cctcgatcag cgcccgaagg tcggcaggct gtcgccacat cgtctcgcgg
     2041 aggatgctcg ccatctagcg gctcgtcgcg ccggcggcgg cgagcgactc gtcgagaatc
     2101 gcaagtccgc gctcggtgtc gtcgtcggag aggttgaggg gcgggagaaa gcggatcacg
     2161 tttccgtaga ggcccgcggc gaggagcagc agaccgcggt cgtacgcctc gctaatcgtt
     2221 cgggccgtga ggtcgctcgc gggagccttc gtgccgtggt cggtgacgag ctcgattgcc
     2281 agcatcgggc cgaggccgcg cacatcgccg atctcctcga accgggcctg catggcgtct
     2341 agcgctcccc gcagtcgctc gccgagcgcg gttcctcgag caagcggctc cggctgcgag
     2401 agctggtcga gcacgaccgc cgcagccgcg caggagaccg ggttgccgcc gaacgtcccg
     2461 ccgaggccgc ccacgtcggg ggcgtccatg atctcggccc gcccggtgac cgccgccagc
     2521 ggcaggccgc cgccgagcgt cttcccggag atgagcaggt cgggctcgac gccgtagtgc
     2581 tcgatcgccc acatgcgccc ggtgcgcccg cagcccgact gcacttcgtc gtcgacgtag
     2641 aggatgccgt ggccggcgag cagctcctgc agtcgggccg ggaagtcgtc tgtcatcggg
     2701 atgaagccgc cctctccctg gacgggctcg agcaccgcgc aggcgacggt cgcggggtcg
     2761 agatcgctct tgaagagccg ctcgatcccc gccagggcat cgtcgctcga gacgccgcgg
     2821 tacggatacg gcgcgggcgc gcggtacacc tccggcgcga acggcccgaa gcccttcttg
     2881 tagacgagct tcgacgtcat cgtcatgcag agcagcgtgc ggccgtggaa ggcgtagtcg
     2941 aagacgagca ctgcaggccg cttcgtatac gcccgcgcga ccttgaccgc gttctctacg
     3001 gcctccgcgc cggagttcac gagcacgctc ttctgcgggc cggggcaggg cgacagctcg
     3061 gcgagccgcc ggcagacgtc tacgtacggc tcgtacgagc cgactgcgac catctggtgc
     3121 aggaagcggt cgacctggtc gtggatcgcc tcgacgatcg ccgcgtgacc gtggccgagg
     3181 ttctggcagc cgatgccgcc gacgaagtcg aggaactcgt gaccgtcggc atccgtgatg
     3241 cgggcacctt cagcgcgcga gacgaccagg cgcggagtgg ggacggcgcg cgaaacgtag
     3301 tgctcgcgct cctcgatcag agactgcgcg gcggacttct cctgcaatga catggcgaca
     3361 accttccggc ggaagtgggc ttcggccgat tatcgagacc gacacacggc ggagcaacac
     3421 ggagccacct cgtcacgggc tgaagaacgg tgttctaatt actcagagtc cggccgtgcc
     3481 ggagccggga cggccctcgg gaggaggaga gagagtgcga acgatcgaca tcaatgccga
     3541 ctgcggggag agcttcggca actggacgat ggggaacgac gaggcgttga tcccgttgat
     3601 cacgtccgcg aacgtcgcct gcggctacca cgcgagcgat ccgttgacga tgcgcgcgac
     3661 cgttcgggcc tgcgtggcga atggtgtcgc ggtcggctcc catcccggct tccccgacct
     3721 cctcggcttc ggccgccgcg tgatgcagat ctcccctgag gatgcgtatg cgtacgtcgt
     3781 ctaccagacc ggggcgctgc aggccttcct ggctgccgaa ggcgcgactc tgcaccacgt
     3841 caaggcacac ggcgccttct acacgatctt gcgcgaggac gacgatctcg cggcggcgtt
     3901 ctgcgaggcg gtcgaggcgg tcatgcccga gccgctcgtc tactggcccg acccgaccga
     3961 ctgctcgctg ccgcgggccg ccaggcagcg cggcctgcgc gtggtgctcg agatctaccc
     4021 ggacctcgac tactcgccgg aggggatgct cgtcctccag cgggcgaagc atcaccgtga
     4081 cgtggcccaa gcagctggac aggtgcgccg ctggatcgag accggggagg tcgaggcgct
     4141 cgacggctcg acgatcccgg tcgacggcga gagcatctgc gtgcacggcg acgggccgaa
     4201 tgtgaccgag atcacgacgg cgattcgcgt cgtgatcgag gagagcggca accaagtcgg
     4261 gcagccggtg atctgacacc gtgggcttca gcaaggttct gatcgcgaac cgcggcgaga
     4321 tagccgtgcg catcatccgc accctccgcg agatggggat cggctccgtc gccgtctact
     4381 ccgaggccga cgccgaggcg cctcacgtcc gctgggcgga cgaagcggtc gcgatcgggc
     4441 cgggcccggt cggcaagagc tacctcgacg cagacaagat catcgccgcg gcgcgcgaga
     4501 ccggagcagg cgccgtccat ccgggatacg gcttcttcgc ggagcatccc gagttcgccg
     4561 ccgcgtgcgg tgatgccggt ctcgtgtggg tcgggccgcc gccgcaggcg atcgaggcga
     4621 tggggctgaa gacccgtgcc cggcggctga tggaggaggc gggtgttccg gtcgtgcccg
     4681 ggacgatcac gcctgctcac tcgccggcag aggcgcttgc cacagcccgc gacatcggct
     4741 ttcccgtggc gttcaagact gcgggcgggg gcggtggaaa ggggtttcac gtcgcgtact
     4801 cggaagcgga cgccgcttcg gcgttcgagc gggcgagcac cgaaggcgag cgcttcttcg
     4861 ccaatcccga cgtctacgtc gaggagtacc tcgaagaccc ccggcacgtg gagatccagg
     4921 tgctcgcgga cacgcaggga aacgtcgtcc atctcttcga gcgtgactgc tccgtgcaac
     4981 gtcggcacca gaagctcatc gaggaggcgc cggcaccgac gatcgacgag gacacccgca
     5041 cacggatctg cgcgatcgcg gtcgacgccg cacggtcggt cggctacgtc tccgccggca
     5101 cggtcgaagg gctcctcgtc ggcgaccgct tctacttcct cgagatgaac accaggctcc
     5161 aggtcgagca tccggtcacg gagctcctga ccggcatcga catcgtccgt gagcagatcc
     5221 gcgttgccgc gggtgagccg ctctcgttcg cgcaggagga catcgtgccg cggggggccg
     5281 cgatcgagtg ccgcgtcaac gccgaggcgg cgcacctcga cttcctgccg ggcccgggga
     5341 cgatcaccgc ctaccacgag ccgggtggac cgggggtgcg ggtcgactcg ggcattcgcg
     5401 cgggctactc ggttcctccc ttctacgatt ccctgctggc caaggtggtc gtctgggact
     5461 ggtcacggga agccgccacg gcgcgcatgc tgcgtgcgct cgccgagtac gagatcgagg
     5521 gtctcgccac cctgctgccg ttccatcggt cctttctcgc cacccgtcaa tggcgtgagc
     5581 tcgaaaccgg tcgtgacttg ctcggcgacc cggagtggct tgccgcgacg gcgccgtagc
     5641 gagcgtcggt tcacgtttgg tgcgatgagg gccacccttt agggttgcgc cgtgcccgac
     5701 accggctcag ccaagctcgt cgagcccccg ctgccagacc ctcggtactc gttcggcggc
     5761 gacgagttca tctactgcga gatctcggag gagatgagct tccacgccaa cttcaagggt
     5821 ctcgcgatct gcctcgagct gcagcagcgc gcgctccggg gggtggtcga gatctgcccc
     5881 tccaacgcct cgtaccaggt gcggatcgac ccggacgtgg tggccccccg cgagatgctg
     5941 gcgctgctcg aggagctcta cgagtcggtg gacgccgaac atgcccactt caagtcgcgg
     6001 gtcatagacg tacccgccta ctacaacgat ccgtggacgc gcgaagtgct gatgaacttc
     6061 cgcgaccggc atcagaaccc gaccggaacg gatctcgagt acgtcacgaa ggtgaacggg
     6121 ttcgcgagcg aggacgagtt catcgaggcc caccaccgcg cgccctacta cgtgtccatg
     6181 gtcggcttcg taccgggtct cccctggtgc ttccagatgg ttccgcgcga gcggatgctc
     6241 gaagtcccga agtacatccg cccgcgcacc gacacgcccg cgctcgccct ctcacacggg
     6301 ggcgcgttcg ccgccatcta tccggtgcga gggcccggcg gctaccagct cttcgccatc
     6361 tgcccggtgc cgatcttcga ccccaagcag gaactgcccg acttccagga gtcgttcgtc
     6421 gtcttccgga cgggtgacat catcaagatc cgcccgatcg accgcgagga gtacgacgcc
     6481 atccgcgcgg aggtggaggc gaagacctat cgctaccgga tggtcgacgt ggacttcgag
     6541 ccgggagcct tcttcgacga ccctgacgcg tacaacgccc gcctgctcga ggagctctac
     6601 cggtgattcg cgtccgcgaa ggcggcgtct cgacgacagt gcaggacgca gggcggttcg
     6661 gtctctacca cctcgggatg ccgccgtccg gggcgctcga cgggtactcg taccgcgttg
     6721 cgaacctgct cgtcggcaac gacgagggtg cagcggtgct cgaggcgacc ttcaacgggc
     6781 ccacgctgga gttcgagcat gacgccgtcg tcgccgtcac cggcgccgat cttccgccgc
     6841 tgctcgaggg gagcgagatg ccgcagtgga cggcgttcgc cgtccaggcc ggacagacgc
     6901 tctccttcga ctatctgcgg gccggcgcgc gcgcctacat cgccgcggcg gggggcttcg
     6961 acgtgcctgt tgtcttcggc agccgctcga cctacgcgct gtgcgcggtc ggcggcgtcg
     7021 acgggaggcc gatccacgaa ggagacacgc ttccggtcgg cgacgcgccc gagtccccgt
     7081 cgctcgaggg ccgcacggtc gcgccggggc tccgtcccga gttcgcccgg gagtgggaga
     7141 tccgcgtgat catggggctc tgtgactatc gcctgacgga ggaggggcgc cgggcgttcc
     7201 tcgagtccga gtgggtggtc acaccggcgg ccgaccggat cggctatcgc tacaaggggc
     7261 ccgagctcga gttcgtcgag cgggagcagc ctttcggagc cggcagcgac ccctcgaacg
     7321 tcgtcgacgc cccgtatccc gtcggctcga tccaggttcc cggcggggtc gagccgatcg
     7381 tgttgctgaa cgatgccgtg acgggcggcg gctacgtgac catcggcacc gtgatcagcg
     7441 ccgacttcgt ccgcctggcc catgcgaaga cgcacgacaa gacgcatttc cgcggcgtcg
     7501 acctcgacga ggcactcagc gcccgagccg agggccgggc ccggctcgac gaggtccggg
     7561 cgcgcctcgg tgcgtgagag aggggagagg gaggagcgcg atgagcgagc agaagacgat
     7621 acggtcgccg ctgccgggga cgttctatcg ccggcccaat cccgacgccg agccgttcgt
     7681 gagcgacggc gacgccgtcg agccgggcga cgtcgtcggc ctcgtcgagg tgatgaagac
     7741 attccacgag gtcaaggccg ccgacgcggg agtggtcgag cggttcctcg tcgagaacga
     7801 ggatcccgtc gacgccgggc aggagatcgt cgcgctccgc gacgcctagg ccgatggcgc
     7861 cgctaggcgc cgctaggcgc cgcggccgca gtcgatcagg tcgaggacgt cctcgggccg
     7921 tagcggcgtc ttctccactt ctgcgccgaa cggcgcgagc gcgtccgcga tcgcgttcac
     7981 gatcgccgcc ggggctccga tcaggccgcc ttcgcccatg cccttgaagc ctcctgccgt
     8041 gacgggggac ggggtctcga ggtggtggat ctcgggcgcc ggtacctctg tcgcagtcgg
     8101 gtagaggaag tccgcgagcg tcgcggcgag gaactggccg tcctcgccgt aagggagccc
     8161 ctcgtagagc acggcgccga ctccctgggc gatcgctccg gccagctggc cgtcgacgat
     8221 catcggattc agcaccgtgc cgcagtcctc gacgcagacg atgcgctgca cgtcgacgac
     8281 gccggtctcg gggtcgacct cgacgaccgc gacgatgcag gcgttcgagt acgtctccgg
     8341 cgggtcgtag ctccgcgtcg cggcgggaat cgggtcgact ccctccggtc gcgggcccca
     8401 gtacgctgct gcggccacct cggccatcgt cttcgaccga aacggcgcgc ccttggcaga
     8461 gatgacgccg ccggcgatct cgaggtcgtc ggcgctcgac tcgagcagat cggaggcgac
     8521 ggcgaggagc ttcgtgcgga cgtcttccgc ggcgagcatg agcgccccgc tcgcgacgac
     8581 ggcggtgcgg ctgccccagc tcccggtcgc ataggcggtt gcagcagtgt caccctgcac
     8641 gacacggacg tcctcgaacc gaacgcccag ggcgtcggcg gcgagctgcg ccatcgtcgt
     8701 ctcgtgcccc tgcccattgg actggaagcc ggaggcgacc gtgacggttc cgtcggtctc
     8761 catggtcacg gtcacggcgt cggtgtagtg gaagccggga aagcccatcg cccgcgcgat
     8821 ctcggcagcc catgctccct gctcgacaaa ggggctgaag ccgacaccga cgtaacggcc
     8881 ctgctcgcgc agccgcccct gctcctcgcg gaatgcctcg tagccgacgg cgtccgcggc
     8941 gacgcgcatc gactccgaga agctgccgcc gtcgaagtgg cagtcggtga gagtcgtgaa
     9001 gggctctccg tccgggatcg cattgcgcaa acgcagctcc agcgggtcga tcgcgagctc
     9061 tcgcgcgatg tcgtcgacga gcgcttcgcg ggcggtgtga cccggcgtcc agccgacacc
     9121 gcggtacgcg acgctctggc acttgttcgt catcgccgcg tcgacctcgt agcgaaccgc
     9181 gtcgagtcgg tagaagctcg gcaggaggac ggccgcgcag agcgggtcga tcagactcga
     9241 ccatggatgt ccctggtacg cgccgccgtc accgacgtag tgaccggtga gggcgaggaa
     9301 acgcccgtcg gccgcaaccg cggcctcgag ctcgacgacg acctccttcg agtgtccact
     9361 ggcgaggagc gcctcgagcc gatcctcgac ccacttcacc ggcgagccga gccgccgtgc
     9421 cgcgaggacg acggccacct cttcgggata caggtgcacc ttgaggccga agccgccgcc
     9481 gacgtgcggt gtcagaacgc gcacgtcgcc ttcgcgcacg ccgagcaggc cggcgagcat
     9541 ccagcgggtg aggaagggca tctggcccga cgaccagacg gtaaggccgc ctgcagcccc
     9601 ccac
//
LOCUS       DBNGNK010000010         9058 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1863758,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000010 DBNGNK010000000
VERSION     DBNGNK010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9058)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9058)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9058
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1863758"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC150V_00205"
     CDS             complement(<1..586)
                     /locus_tag="AC150V_00205"
                     /inference="COORDINATES: protein motif:HMM:NF052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-apionate lactonase family protein"
                     /protein_id="HIR3761377.1"
                     /translation="MTTTDHYRLLIGQEEPAPEMRELRAGPVTVLLDGVDLRYARLGS
                     TELVRRVYAAVRDESWNTIPGEVSDVEVDAGEDSFEVRFSVRHRSPALDFSWQGTITG
                     DPGGRIEYVFDGKAEADMLYNRIGLCVHHPWRETAGRPYRATTPDGEVTGSFPSLIGP
                     QGFENGVYLALFPAFERLDLALAGGGSLRFQFEGD"
     gene            complement(591..1616)
                     /locus_tag="AC150V_00210"
     CDS             complement(591..1616)
                     /locus_tag="AC150V_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3761378.1"
                     /translation="MDHTFHVGVTADVRRPDGSLVFAPVDLAALDVPGIEWNFLPVDA
                     RELTPDLLCDLDGLFHFSPVVSADSLDGVERLAILARHGVGLDFIDLDACTQRGIAVT
                     ITPDAITRPMASGAVALILALSHHLVARNSLLRDGRWSEGRFDLLGLGLTGRTLGVIG
                     YGRIGREVARLLRPWEMKVLVATRTPPQDADVAHVELDVLLEQSDVVVVAAPLTGETR
                     HLLDARRLSLLKPSALLVNIARGPIVDQDALVEALREGRIAGVGLDVFETEPIPAGDP
                     LLAFPNVVAAPHAIGYWDQLFSGCVDAACGAILDVAAGRIPADLVNPAVLESTSFRDK
                     LASFERR"
     gene            1664..2059
                     /locus_tag="AC150V_00215"
     CDS             1664..2059
                     /locus_tag="AC150V_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3761379.1"
                     /translation="MKVTEKIVVGELPVGGSEPLISSAVRWGDLLFLSGRAPLDTSTL
                     EVVSDDFEAQTRAVLADIDASLREAGSGPEHVLRVQCYLLSADDFDTWNRLWAEYFPS
                     PRPVRTTIVTGFTVPRMLIEIEVTAGIPS"
     gene            2056..3324
                     /locus_tag="AC150V_00220"
     CDS             2056..3324
                     /locus_tag="AC150V_00220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3761380.1"
                     /translation="MKVVIVGGGVAGLCTAYYLRKRDVDVTVLESRSIASRDAASYGN
                     GGWITPAQAGPLPEPGLTLYGLRALTSVDSPLYFRPSYLPRLVPWLTRFWTYCNEKDY
                     ERGWSAIARLAERVFILVDEMVDDGMEFELHKTGMICATAEPAEAQKVLDGLEPMRSF
                     GFELPTELMLADELHAFEPALSERVQAGFYVAQQWHVRADTFTSALGDAVRKLGADVV
                     EGVRVEGFDTADRRVRAVHTSQGDVSGDAFLLAAGSWTTPLARRLGVKFPMEPGKGYT
                     FLLQPTTMPKYGILFADIHAGASPLADGLRISGTMEFSGYNLELDQRRIDNVFRLARE
                     YIQLDQPEYENAWAGLRPMAADGLPILDWAQPYANTYLATGYSMLGMTLSQPAGEAMA
                     EMITSGRRPELFEPFRVDRFPRTIARRAAS"
     gene            3321..4523
                     /locus_tag="AC150V_00225"
     CDS             3321..4523
                     /locus_tag="AC150V_00225"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3761381.1"
                     /translation="MTGCPEFGSSWDLHEPAFIEDPYPTYTRLRDSCPVLHSDRYMGG
                     FWMLSRHDDVRDAVADWETFTSSVPGVTAIPMIIQRTEPLVPIELDPPLHTRYRQLVG
                     HVFRRQRVEELRPGVTELAARLVAPIVERGKGDLAREFAIEMSTGTLGLFLDLPSEDG
                     PKWVGWIKRLHGSVHDPEDARAATAEIRGYVEQLVAERREHPRDDFISFLLASEVGGE
                     QLSDTSVRDFVHLTINAGFETTAGAMGLSLLELARRPALRRRLFAAPELVPTAVEELL
                     RFTSPVQIFARNAARDVELHGRQIPAGDIVAVGFASANRDPEAFTDPDEIVLDRSPNK
                     HLSFGFGPHVCLGAWVARLELQVMLAVFAERLPEFHLDPEGTIVRKGRGDQIALESLP
                     VIVGPAPA"
     gene            complement(4540..5703)
                     /gene="dgoD"
                     /locus_tag="AC150V_00230"
     CDS             complement(4540..5703)
                     /gene="dgoD"
                     /locus_tag="AC150V_00230"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015814691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HIR3761382.1"
                     /translation="MRVTAVDTVVVNARLRNWVFVKVTTDEPGLVGWGEASLEWKSRA
                     VVGAVDDLSSLVVGEDPMRLEHLWQVMFRQQFFRGGIVTMSAISGIDQALHDIKAKAL
                     GVPLYQLLGGRVRDRVRLYDHLGGGDSTAVYDAATPEAFAASARASVADGFTALKVLA
                     VPPGRALEGAASLRHAEALLGAVRDAVGEDVDVVVDLHGRTTAAAAIQYGLVLAPYRP
                     WFLEEPVQPGNVDALVEVARALPVPIATGERLVTRWEFREVLEKRACAVVQPDVCHCG
                     GVAELRRIASHAETHYIGVAPHNPLGPIATAVNVHLALATPNFLIQEVMRSDVPWRDD
                     VVDAPLAIVDGHVLPPERPGIGVDVDEAAAARHPYEPELEARAFQPDGSVADW"
     gene            complement(5708..6466)
                     /locus_tag="AC150V_00235"
     CDS             complement(5708..6466)
                     /locus_tag="AC150V_00235"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761383.1"
                     /translation="MDDRVAIVTGGGSGIGAATARLLAEEGQTVALVGRRPDAVEGIA
                     AEIVAAGGSAFALPADLSSPRAPWEIVEAVLERTDRLDIVVNNAASFALELVGDTTLE
                     EFDDHIAVNVRAAYFLVHAALPSLRASPAAAVVNVSSAAAAMYRRRQTLYGLTKAALE
                     HMTMNMAAELAPDRIRVNCVRPGPVATEIHTAAVADPAARLAELGRMVPLGRLGEPEE
                     VALWVRHLVALDAEWVTGVVLPIDGGRVLGPPEA"
     gene            6515..7393
                     /locus_tag="AC150V_00240"
     CDS             6515..7393
                     /locus_tag="AC150V_00240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3761384.1"
                     /translation="MRVLVVGAGLMGAQIGCEYALGGHEVTLVARDTSAVDERVRQAF
                     ALVSAEELAPDETVAAAKGRVAIAGRVDEDTAQCDLAVESLPESLEVKVEVFGPIAAY
                     APDAILASNTSSIPITTLAEALGEPERVVGTHYLNPPLLMPTVEVIPGTRTHEWITNE
                     LVDVLRELGKLPVVVRRDVPGFIWNRLQFALLRECAWLVDNGVAGTEDVDLILREGLA
                     RRWRRVGPFAAIGLGGLHTWSLSAANLVPELSTTSDLGDLARFAPSDMAELERLAAAR
                     DRILAAELRAERSTDG"
     gene            7386..8585
                     /locus_tag="AC150V_00245"
     CDS             7386..8585
                     /locus_tag="AC150V_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR3761385.1"
                     /translation="MAEHGSTVPMTSFDHHDPAFVENPHPVLAEIRENHPLIHSDRYG
                     GFWLLSRYEDVTEAAIDWRRFTSSVVGTTVIPPSQPRDYPQLPIELDPPKHTLYRSIV
                     NPIFAKSRVEALRPDLHELARSLLQPLIERGHGDFVKEFATPMSLGTLGMFMGLPADD
                     QPKWADWVRRMFEGSINDPGEQKRAGAEFQEYLDALVAERKEERRDDFISLLLDTEVE
                     GHRLTDLEIRGFGVVMLMAGHETTAGAMSMTTKFLAEEPEIRSRVFAAPELIPSAVNE
                     FLRLFPSIQIFCRNAVEDMEIHGAEVKMGEVVALAYAAANRDPHEFSEPDECVLDRKP
                     NRHVTFGYGHHLCLGANVARLEMAVMLAELARLMPDFALDPERNPVWRARGDVRGLLS
                     LPLVLAD"
     gene            8588..>9058
                     /locus_tag="AC150V_00250"
     CDS             8588..>9058
                     /locus_tag="AC150V_00250"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3761386.1"
                     /translation="MPAHETVRALVLERLGGVPRVETLEVPEPGAGEVRIRMLATGVC
                     HTDIAAPRDAFAAPLVLGHEGCGEIESAGDGVGLAPGTRVVLSFRTPCGRCRQCLAGR
                     PEVCAHGLDLREGRSRWRGEPIPGLLSLGCFSELVVVPSAAAVPIDKTLPAEQAA"
BASE COUNT         1402 a         3176 c         3113 g         1367 t
ORIGIN      
        1 ggtctccttc gaactggaag cgcagcgagc cgccgccggc gagcgccagg tcgagacgct
       61 cgaaggccgg gaagagcgcc agatagacgc cgttctcgaa gccctgcgga ccgatcagcg
      121 atgggaagga gccggtgacc tcgccgtccg gcgtcgtggc tcggtacggg cgtcccgcgg
      181 tttcccgcca ggggtggtgc acgcagaggc cgatgcggtt gtagagcatg tccgcttccg
      241 ccttgccatc gaacacgtac tcgatccgcc cgccggggtc gccggtgatc gtgccctgcc
      301 acgagaagtc cagcgccggg ctccgatgcc ggaccgagaa tcgaacctcg aacgagtctt
      361 cgcccgcgtc gacctcgacg tccgagactt cgccaggaat cgtgttccag ctctcgtcgc
      421 gcactgccgc atagacgcgg cgcacgagct cggtgctccc gaggcgcgcg tagcggaggt
      481 cgacgccgtc gagcagcacg gtaacggggc cagcccggag ctcacgcatc tccggggcgg
      541 gctcttcctg gccgatcagc agacggtagt ggtcggtagt ggtcacgctc ttatctcctc
      601 tcgaagctgg caagcttgtc acggaacgac gtggactcga gaacggcggg attgacgagg
      661 tcagccggga tccgccccgc cgccacgtcg aggatcgcac cgcaggcggc gtccacgcag
      721 ccgctgaaca gctgatccca gtagccgatc gcgtgcggcg cggccaccac gttcgggaag
      781 gcgagcaggg ggtctccggc gggaatcggc tccgtctcga agacgtcgag cccgacgccg
      841 gcgatgcgcc cctcgcggag cgcctcgacc agcgcatcct gatccacgat cggaccccgt
      901 gcgatgttga cgagcagggc cgacggcttc aacagcgaca gtcgtcgggc gtccagcaga
      961 tgacgggtct cgccggtgag cggagccgcg acgacgacga cgtcggactg ctcgagcagc
     1021 acgtccagct ccacgtgcgc cacgtccgcg tcctgcgggg gcgttcgcgt ggcgacgagc
     1081 accttcatct cccacggccg cagcaggcgg gcgacctctc ggccgatgcg cccgtacccg
     1141 atcacgccca acgtacgccc ggtgaggccg aggccgagca ggtcgaagcg accctcgctc
     1201 cacctgccgt ctcgcaggag gctgtttcgg gcgacgagat ggtgtgacaa ggcgaggatc
     1261 agcgcgacgg cgccggacgc catcgggcgc gtgatcgcgt ccggcgtgat cgtcacggcg
     1321 ataccgcgct gcgtgcaggc gtcgagatcg atgaagtcga gcccgacgcc gtgccgcgcg
     1381 agaatcgcga ggcgctccac gccgtcgaga ctgtcggccg acacgaccgg cgagaagtga
     1441 aagaggccgt cgaggtcgca gagaaggtcg ggggtgagct ctcgcgcatc gaccggcagg
     1501 aagttccact cgatgccggg cacgtcgagc gcggccaggt cgaccggagc gaacacgagc
     1561 gagccgtcgg gacgtcggac gtctgctgtg acgccgacgt ggaatgtgtg gtccatttgc
     1621 attccgtatg atcgattctc ccagacccaa gagagtgagg tgcgtgaagg tgaccgagaa
     1681 gatcgtcgtc ggcgagctcc cggtgggagg ctccgagccc ctgatctcga gcgcagtgcg
     1741 gtggggcgac ttgctcttcc tgtccggccg ggccccgttg gacacgagca cgctggaggt
     1801 cgtcagcgac gacttcgagg cgcagacgcg cgccgtgctg gccgacatcg acgcctcgct
     1861 gcgtgaggcg ggctccggtc cggagcacgt gctccgcgtg cagtgctacc tgctctcggc
     1921 agacgacttc gacacgtgga atcgcctctg ggccgagtac ttcccttccc cccgtcccgt
     1981 tcgaaccacg atcgtcaccg ggttcaccgt gccgcggatg ctgatcgaga tcgaagtcac
     2041 cgccggaatc ccatcatgaa agtggtcatc gtggggggag gcgtcgccgg actctgcacg
     2101 gcgtactacc tgcgcaagcg ggacgtcgac gtcactgtgc tggaaagccg cagcatcgct
     2161 tcgcgggacg cggcgtcgta cggcaacggc ggctggatca cacccgcgca ggccggcccg
     2221 cttcccgagc ccggcttgac gctgtacgga cttcgtgcgc tcacgagcgt cgactcgccg
     2281 ctctacttcc gccccagcta cttgccacga ctggtgccct ggctgacgcg cttctggacg
     2341 tactgcaacg agaaggacta cgagcgcggc tggtcggcca tcgcgcggct cgcggagcgt
     2401 gtcttcatcc tggtggacga gatggtggac gacggcatgg agttcgagct gcacaagacc
     2461 ggcatgatct gcgcgaccgc cgagccggcc gaggcccaga aggtgctcga cggcctggag
     2521 ccgatgcgat cgttcggctt cgagctcccc accgagctga tgctcgccga cgagctgcac
     2581 gctttcgagc ccgcgctctc ggagcgcgtt caggccggct tctatgtcgc gcagcagtgg
     2641 cacgtgcgtg ccgacacgtt cacgagcgcg ctcggagacg ccgtccgcaa gctcggtgcc
     2701 gacgtcgtcg aaggtgtccg agtggagggc ttcgacaccg ccgaccgccg cgttcgcgcg
     2761 gttcacacct cacagggaga cgtctcgggc gacgcgttct tgctcgccgc cggctcctgg
     2821 accaccccgc tcgcccgccg gctcggtgtc aagttcccca tggagcccgg caagggctac
     2881 accttcctgc tccagcccac cacgatgccc aagtacggca tcctgttcgc cgacatccat
     2941 gcgggcgcgt cgccgctcgc cgacgggctg cgcatctcgg ggacgatgga gttcagcggc
     3001 tacaacctcg agctcgacca gcgccgcatc gacaacgtct tccgcctcgc ccgcgagtac
     3061 atccaactcg accagcccga gtacgagaac gcgtgggcgg gcttgcggcc gatggccgcc
     3121 gacggcctgc cgatcctcga ctgggcgcag ccctacgcca acacctatct cgcaaccggc
     3181 tactcgatgc tcggcatgac cctgtcgcag cccgccggtg aggcgatggc cgagatgatc
     3241 accagcggcc ggcgaccgga gctcttcgag ccgtttcgcg tcgaccgatt cccgcggacg
     3301 atcgcgcgcc gagcggcgtc gtgacgggct gcccggagtt cggctcgagc tgggatctgc
     3361 acgagccggc gttcatcgag gacccgtacc ccacctacac ccggctgcgc gactcgtgcc
     3421 cggtgctcca ctcggaccgc tacatgggcg gcttctggat gctgagccgc cacgacgacg
     3481 tccgggacgc cgtcgccgac tgggagacct tcacctcctc ggtacccggc gtgacggcga
     3541 tcccgatgat catccagcgc accgagccgc tcgtcccgat cgagctcgac cccccgctgc
     3601 acacccggta caggcagctc gtcggccacg tcttccgccg ccagcgcgtc gaggagctgc
     3661 ggcccggggt gaccgagctg gccgcccggc tcgtggcccc gatcgtcgaa cggggaaagg
     3721 gagatctcgc gcgggagttc gcgatcgaga tgtcgaccgg cacgcttggc ctcttcctcg
     3781 acctgccgag cgaggacggc ccgaagtggg tcggatggat caagcgcctt cacggctccg
     3841 ttcacgaccc cgaggacgcg cgcgcggcga ccgcagagat ccgcggctac gtcgagcagc
     3901 tcgtcgccga gcgccgggag cacccccgcg acgacttcat ctcgttcctg ctggcttcgg
     3961 aggtcggcgg cgagcagctg tccgacacct ccgttcgcga cttcgtccac ctcacgatca
     4021 acgcgggctt cgagacgacg gcgggcgcga tgggcctgtc cctgctggag ctcgcccgcc
     4081 gacctgcact gcggcgtcgg ctgttcgcgg ccccggagct tgtcccgacg gcggtcgagg
     4141 agctgcttcg ctttacgtca ccggtccaga tcttcgcgcg gaacgccgcc cgcgacgtcg
     4201 agctgcacgg gcggcagatc ccggcggggg acatcgtcgc cgtcggcttc gcctccgcga
     4261 acagggatcc ggaggctttc accgatccgg acgagatcgt tctcgaccgc agcccgaaca
     4321 agcacctttc cttcgggttc gggccgcacg tctgcctcgg cgcctgggtg gcgcgactcg
     4381 agctgcaggt catgctcgcc gtcttcgcgg agcgcctgcc cgagttccat ctcgatccgg
     4441 agggcacgat cgtgcgcaag ggaagaggcg accagatcgc gctcgagtcg ctcccggtga
     4501 tcgtcggccc cgctcccgcg tagagcagcc aagcgcgtct caccaatcgg cgacggagcc
     4561 gtcgggctgg aaggcgcgcg cctcgagctc cggctcgtag ggatgacgag cggccgcagc
     4621 ctcgtcgacg tccacgccga tgcccgggcg ctcgggagga agcacgtgcc cgtcgacgat
     4681 cgcgagcggc gcgtcgacca cgtcgtcacg ccagggcacg tcagagcgca tcacctcctg
     4741 gatcaggaaa ttcggcgtcg ccagagccag atggacgttg accgcggtcg cgatcgggcc
     4801 gagcggattg tgcggcgcga cgccgatgta gtgggtctcg gcgtgggacg cgatccggcg
     4861 gagctctgcg acgccgccgc agtggcagac gtcgggttgc acgacggcgc acgcgcgctt
     4921 ctccagcacc tcgcgaaact cccatctcgt caccagccgc tcaccggtcg cgatcggcac
     4981 cggcagggcc cgtgcgacct ccacgagcgc gtcgacgttg ccgggctgaa ccggctcctc
     5041 gagaaaccat ggccggtagg gagcgaggac gaggccgtac tggatcgccg ccgccgccgt
     5101 cgtgcggccg tgcaggtcga ccaccacgtc gacgtcttca ccgaccgcgt cgcggacggc
     5161 gcccagcaac gcctcggcgt gccggagcga tgcagcaccc tcgagggcgc gccccggcgg
     5221 gacggcgagg actttcaacg cggtgaagcc gtctgcgacg ctcgcccggg cgctcgccgc
     5281 gaacgcctcg ggcgtcgccg cgtcgtacac ggcggtgctg tcgccgccgc cgagatgatc
     5341 gtagagacgc acgcggtcgc ggacgcggcc gcccaggagc tggtacagcg gcacgccgag
     5401 cgccttcgcc ttgatgtcgt gcagcgcctg gtcgatgccg ctgatggcgg acatcgtgac
     5461 gatgccgccc ctgaagaact gctgccggaa catgacctgc cacaggtgct ccagccgcat
     5521 cgggtcttcg ccgacgacga gagacgagag gtcgtcgaca gccccgacga ccgctctgga
     5581 cttccactcg agcgaagcct ccccccaacc gacgaggccg ggctcgtcgg tggtcacctt
     5641 gacgaagacc cagttgcgca gccgggcgtt gacgacgacg gtgtcgaccg cggtgacgcg
     5701 cacggcgtca ggcttcgggc gggccgagaa cacggccgcc gtcgatcggc agcaccacgc
     5761 cggtcaccca ctcggcgtcg agtgcgacca ggtggcgtac ccagagcgcg acctcctcgg
     5821 gctccccgag ccggccgagc gggaccatgc gcccgagctc ggcgagccgg gctgcagggt
     5881 cggcgacggc cgcggtgtgg atctcggtgg ccaccggccc ggggcggacg cagttcacgc
     5941 ggatccggtc gggcgcgagc tcggctgcca tgttcatcgt catgtgctcg agcgccgcct
     6001 tggtgagccc gtagagggtc tggcgtcggc ggtacatggc cgcagcggcc gaggagacgt
     6061 tcacgaccgc cgccgcgggc gaggcgcgca gcgacggcag tgcagcgtgg acgaggaagt
     6121 aggcggcacg cacgttgacc gcgatgtgat cgtcgaactc ctcgagcgtg gtgtcgccga
     6181 cgagctcgag cgcgaagctc gcagcgttgt tgacgacgat gtcgaggcgg tcggtccgct
     6241 ccagcaccgc ctcgacgatc tcccagggcg cccgcggcga ggacaggtcg gccgggagcg
     6301 caaaggcgct tccgccggca gcgacgatct cggccgcgat gccctcgacg gcgtcgggcc
     6361 ggcggccgac gagcgcgaca gtctggccct cctcggcgag gagccgcgcc gtggcggcgc
     6421 cgatgcccga cccccctcca gtgacgatgg cgactcggtc gtccaaattg gtcggacaat
     6481 actatgatca tcaaggtttg cgaggagggt cgagatccga gtcctagtcg tcggcgcggg
     6541 cctgatgggc gcccagatcg gctgtgagta cgcgctcggc ggccacgagg tcactctcgt
     6601 cgcgcgcgac acctccgccg tcgacgagcg cgtgcggcag gcattcgcac tcgtctccgc
     6661 agaggagctg gcacccgacg agacggtggc ggccgcgaaa ggccgcgtcg cgatcgcagg
     6721 ccgggtcgac gaggacaccg cgcagtgcga tctcgccgtc gagtcgcttc ccgagagcct
     6781 cgaggtcaaa gtcgaggtct tcggcccaat cgccgcctac gcgcctgacg cgatcctcgc
     6841 ctcgaacacg tcgtcgatcc cgattacgac cctcgcggaa gcgctggggg agcccgagcg
     6901 ggtggtcggc actcactacc tgaacccccc gctcctcatg ccaacggtcg aggtgatccc
     6961 aggaacaagg acacacgaat ggatcacgaa cgagctcgtc gacgtcctac gggagctggg
     7021 caagctcccg gtcgtcgtgc gccgcgacgt ccccggcttc atctggaacc gactccagtt
     7081 cgcccttctg cgcgaatgcg cgtggctcgt cgacaacggc gtcgcgggaa ccgaggacgt
     7141 cgatctcatc ttgcgcgagg ggctcgcgcg caggtggcgc cgcgtcgggc cgttcgccgc
     7201 aatcggcctc gggggccttc acacctggtc gctgtcggcc gccaatctcg tccccgagct
     7261 ctcgacgacg tcagacttgg gcgatctcgc acgattcgcg ccgagtgaca tggccgagct
     7321 cgagcgcctc gccgcggcac gcgatcggat cctggcggcg gaacttcgag cggaaaggag
     7381 caccgatggc tgagcacgga agcaccgtcc cgatgaccag cttcgaccat cacgatccgg
     7441 cgttcgtcga gaacccccac cccgttctcg ccgagatccg cgagaaccac cctctgatcc
     7501 acagcgaccg ctatggcggc ttctggctcc tctcccgcta cgaggacgtc accgaagcgg
     7561 cgatcgactg gcggcgcttc acgtcgagtg tcgtcggaac gacggtgatc ccgccgagcc
     7621 agccgcgtga ctatccgcag ctcccgatcg agctcgaccc tcccaagcac acgctctacc
     7681 ggagcatcgt caatccgatc tttgccaaga gccgggtgga ggcgctgcga cccgatctcc
     7741 acgagctggc ccgatctctt ctgcagccgc tgatcgagcg cgggcacggc gacttcgtca
     7801 aggagttcgc cacgccgatg tcgctcggga cgctcggcat gtttatgggg ctcccggccg
     7861 acgatcagcc gaagtgggcc gactgggtcc ggcgcatgtt tgagggctcg atcaacgatc
     7921 ccggggagca gaagcgggct ggagcagagt tccaggagta cctcgacgcg ctcgtggcgg
     7981 agcgcaagga agagcgtcgg gacgacttca tcagcctgct gctcgacaca gaggtcgagg
     8041 ggcatcggct caccgatttg gagatccgcg gcttcggcgt cgtcatgctg atggccgggc
     8101 acgagaccac ggccggcgcc atgtcgatga cgaccaagtt cctcgccgag gagcccgaga
     8161 ttcgcagcag ggtcttcgcc gcgccggagc tgatcccctc ggcggtaaac gagttcttgc
     8221 gactgttccc gtcgatccag atcttctgtc gcaatgcggt cgaggacatg gagatccacg
     8281 gggccgaggt gaagatgggc gaggtcgtcg cgctcgccta cgcggccgcc aatcgagatc
     8341 cgcacgagtt ctcggagccg gacgagtgcg tgctcgatcg caagccgaac cgccacgtca
     8401 cgttcggcta cggccatcac ctctgcctcg gtgccaacgt cgcccgcctg gaaatggcgg
     8461 tgatgctggc cgagctcgcc cggctgatgc ccgacttcgc actcgatccg gagcgcaatc
     8521 ccgtctggcg cgcccgcggt gacgtccgcg gacttctgtc gctgccgctg gtgctcgccg
     8581 actgagcgtg ccggcgcacg aaaccgtccg cgcactcgtg ctcgagcgcc tcgggggcgt
     8641 gccccgcgtc gagacgctcg aggtgccgga gcccggcgcg ggggaggtgc ggatccgcat
     8701 gctggccacg ggcgtctgtc acaccgacat cgcggcaccg cgcgacgcct tcgcggcacc
     8761 gctggttctc ggccacgagg gctgcggtga gatcgagtcc gccggcgacg gggtcggcct
     8821 cgcgcccggc acgcgcgtcg tgctgtcgtt caggactccc tgcggccggt gccgccagtg
     8881 cctcgccggc cggccggagg tctgcgcgca cgggctcgac ctgcgcgagg ggcgctcgcg
     8941 gtggcgcggc gagccgattc cgggtctcct ctcgctcggc tgcttcagcg agctcgtcgt
     9001 cgtcccgtcc gctgccgccg tgccgatcga caagacgctc cccgccgagc aggccgcg
//
LOCUS       DBNGNK010000011         5518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_789226,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000011 DBNGNK010000000
VERSION     DBNGNK010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_789226"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1047)
                     /gene="mraY"
                     /locus_tag="AC150V_00255"
     CDS             complement(34..1047)
                     /gene="mraY"
                     /locus_tag="AC150V_00255"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR3761387.1"
                     /translation="MVRVLIAGLIAMVIAVVVGPKFIEWLRRSGVGQQIREEGPARHI
                     VKQGTPTMGGLLILVAALLPFVALSLYTLPGVALIFVTLGCAAIGFADDYLKVRHRRS
                     LGLSGRWKLLLLALITVGVGWVVSEVGYLDTEIYFPVVDVNIDLKWLYFPFLFIIIAG
                     TANGVNLTDGLDGLAAGTSAIAIVTFVAIAAISWIRSGPVGGRSDDYLDVAIFAAALI
                     GGVIGFLWFNAFPAEVFMGDTGSMALGGAIAGLAIMTETEVLLIFIGGIFLIEALSVM
                     IQVASFKRTGRRVFLMAPIHHHFEMKAWSETKIMVRFWIVGAILCAIGFVLSYRYFLR
                     FQL"
     gene            complement(1058..2326)
                     /locus_tag="AC150V_00260"
     CDS             complement(1058..2326)
                     /locus_tag="AC150V_00260"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HIR3761388.1"
                     /translation="MIPVRLAEIEALRLGRLDPGPDDVVVAIGADSRVAARGHLFVAL
                     NTGVDFVTDARARGAATLVPDDQDAALGALASLVRAKSDARVVAVVGSTGKTSTKDIL
                     AAFCSAVVSTIAAEASLNNEIGLPLTVLRLETDTEVLVVEMGMRGLGQIAELCGVARP
                     DLALVTHIGPEHLELVGTVENVARANAEAIAALEPGGIVIAPADALELRPYLDRPDLD
                     IRLFDPESVERVGDRWRFLVHGAAIDLSLPFGQRHMAQNTLAALTAYGALGLPLDRVQ
                     EGADRIRLSRWRGEAVQLPGGGFVVNDAYNANPASMAAALVDLAERAGERRSIAILGE
                     MAELGNSSETHHREIGRRARELGIEVLGVGELGRLYDGRWWAEDAEAAAVLAAEVVRL
                     GDAVLVKASRAIGLEGIPDVLANSAVEWLS"
     gene            complement(2340..3788)
                     /locus_tag="AC150V_00265"
     CDS             complement(2340..3788)
                     /locus_tag="AC150V_00265"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR3761389.1"
                     /translation="MELDVLIGALAPADVIGGGRTEIRDLAHDARRVSAGTLFFCVPG
                     ARADGHAYAVDAVNAGASALVVERPLDVAVPQLVVEDVRSAMAVAADVFFGEPTRELQ
                     VAGVTGTNGKTTTAFLLRSILQHAGRRCGLVGTVSWVVGGELRDAPFTTPEAIELQGL
                     FREMVERGDDAVAVEASSHGSAYRRLDRVRFDALVFTNLSQDHLDLHETMEDYYQAKR
                     RMFVGAHPAPAAVNVADAYGRRLADELRDAHRAPLVTFGATQEAEVAPDDLELGAGGS
                     RFRAAGIELETPLLGAFNVENVLAAVACGLLLDVPDQAIAAGVAAVSGIPGRLEAVDE
                     GQPFTVVVDYAHTPDSLERVLRAARDLGDGRVIAVFGAGGDRDRGKRPEMGAAAVRYA
                     DRAIVTSDNPRSESPLAIIEDVLQGAGVDVEIDPDRRSAIRRALALAEAGDVVVIAGK
                     GHEQGQEIDGVVHPFDDREVAREALREAAAQL"
     gene            complement(3849..>5518)
                     /locus_tag="AC150V_00270"
     CDS             complement(3849..>5518)
                     /locus_tag="AC150V_00270"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3761390.1"
                     /translation="AFVLVFAVMLGRAAWLQVVRGPSFETMADRQHRETIEIPAARGT
                     IYDRTGEPLAIGEQATTIYADPRSVANPQRTARAAAKAFGLSADDLYQEITDKSRGFV
                     YVARKADPYKAAALEKQEHEGLGFYAEELRSYPQREVASTVLGYAGLDNKGLEGLERS
                     LDNVLAGRPGFEVVVKDPFGRAIDVVSGRPERQGQNVVLTIDHQIQANAEQVLSRHVE
                     RWAAKGGTAIVMDPRTGAILAMANAPTFDANLFPATSAERRRNRAVTDVYEPGSTFKI
                     VTISAALEEKIVTPQTSFWLQPTINIADRVIRESHARGSTSMTVKQILSDSSNVGTVT
                     IGQKLGEGTLASWIDRYGFGSKTGVDLPGESPGMVLPLDDWSGSTIGTVPIGQGIAVT
                     PVQMAAAYATIGNGGVRERPHVVDKIGGKRVKPGKGRRIVSKATADQVMAMLRDVVVE
                     GTGTSAAIPGYTVAGKTGTAAKPENGVYVSKYVASFVGLVPAEDPRLVVLVAIDEPKG
                     TIWGGVVAAPVFAEIARFDLQYLEVPPDAPETKEEAALEALGGYSSG"
BASE COUNT          903 a         1934 c         1853 g          828 t
ORIGIN      
        1 ccagcacgag tgcgcggcgc gcaccgatct cgctcatagc tggaagcgga ggaagtaccg
       61 gtaggagagg acgaagccga tcgcgcagag aatcgcgccg acgatccaga agcggaccat
      121 gatcttcgtc tccgaccagg ccttcatctc gaagtggtgg tggatcggag ccatcaggaa
      181 gacacgccgc cccgtgcgtt tgaaactcgc gacctggatc atcaccgaca gggcttcgat
      241 gaggaagatc ccgccgatga agatcagcaa cacctcggtc tcggtcatga tcgcgagacc
      301 ggcgatcgcg ccgccgagag ccatcgaccc cgtgtccccc atgaagacct cagccggaaa
      361 ggcattgaac cagaggaagc cgatcacgcc cccgatgagc gcggcggcga agatcgcgac
      421 gtcgaggtag tcgtcgctgc ggccgccgac cgggcccgag cggatccacg agatcgcggc
      481 gatcgcgaca aaggtcacga tcgctatcgc ggacgtgcct gcagccagcc cgtcgaggcc
      541 gtcggtcagg ttcacgccgt tcgcggtgcc ggcgatgatg atgaagagga aggggaagta
      601 gagccacttg aggtcgatgt tcacgtcgac gacggggaag tagatctccg tgtcgaggta
      661 cccgacctcg gagacgaccc agccgacgcc gaccgtgatc agagcgagga gaaggagctt
      721 ccagcgaccc gacagtccga gcgatctccg gtgccgcacc ttgaggtagt cgtccgcgaa
      781 gccgatcgcg gcacacccca gcgtcacgaa gatgagcgcg acccctggga gcgtgtagag
      841 cgagagcgcg acgaacggga ggagcgccgc gacgagaatc aggagcccgc ccatcgtcgg
      901 cgtgccctgt ttgacgatgt ggcgcgcggg accttcctcg cggatctgct gcccgacgcc
      961 gctgcgccga agccactcga tgaacttcgg gccgaccacg accgcgatca ccatcgcgat
     1021 cagacccgcg atcagtaccc ggaccatgca gcctgagtca gctcaaccat tccacggcgg
     1081 agttcgccag gacgtcgggg atgccttcga gcccgatcgc gcgggacgcc ttgacgagca
     1141 cggcatcgcc gaggcgcacg acctcggccg cgagcaccgc cgctgcctcg gcgtcttccg
     1201 cccaccaacg tccgtcgtag aggcgcccga gctcaccgac tccgagcact tcgatcccga
     1261 gctctcgggc gcggcggccg atctcgcgat ggtgcgtctc ggagctgttc cccagctctg
     1321 ccatctcgcc caggatcgcg atggagcgac gctctcccgc ccgctcggcg agatcgacca
     1381 gcgctgccgc catcgaggcg ggatttgcgt tgtaggcgtc gttaacgacg aagccgccgc
     1441 cgggaagctg cacggcctcg ccgcgccagc gagagagccg aattcgatcg gcaccttcct
     1501 gcacgcggtc gaggggaagc ccgagcgccc cgtaggcggt cagcgcggcg agcgtgttct
     1561 gcgccatgtg gcgctggccg aagggaagag agaggtcgat cgccgctccg tgaacgagga
     1621 accgccagcg gtcgccgacg cgctcgaccg actccggatc gaacaggcga atgtcgagat
     1681 cgggccggtc gaggtagggg cggagctcga gagcgtccgc cggcgcaatg acgatcccac
     1741 ccggctccaa tgcggcgatc gcctcagcgt tcgcgcgcgc gacgttctcg accgtgccga
     1801 cgagctcgag gtgctccggc ccgatgtgcg tgacgagcgc gaggtcgggc cgagccacgc
     1861 cgcacagctc cgcgatctgg cccagtccgc gcatgcccat ctcgaccacg agcacctcgg
     1921 tgtcggtctc cagtcgcagc acggtcagcg gcaggccgat ctcgttgttc aggctcgcct
     1981 cggcggcgat cgtcgacacg accgcgctgc agaacgcggc gaggatgtct ttcgtggacg
     2041 tcttccccgt ggatccgacc accgcgacga cacgggcgtc gctcttcgcc cggacgagcg
     2101 acgccagcgc tccgagcgcc gcatcctgat cgtcggggac gagtgtggcg gcgccgcgcg
     2161 cgcgggcgtc ggtgacgaaa tcgacgccgg tgttcagcgc cacgaacaga tgaccccgcg
     2221 cggccacgcg ggagtcggcg ccgatggcga cgaccacgtc gtcggggccg gggtcgagcc
     2281 ggccgaggcg aagcgcctcg atctccgcga gtcggaccgg aatcatcgcg gctcgctcgt
     2341 cacagctggg cggcagcctc gcgaagcgcc tctcgggcga cctcgcggtc gtcgaacggg
     2401 tgaacgacac cgtcgatctc ctgcccctgc tcgtgcccct tgccggcgat caccacgacg
     2461 tccccggcct cggccagcgc gagggcgcgc ctgatcgcgg agcggcggtc agggtcgatc
     2521 tcgacgtcca ctcccgcccc ttgcagcacg tcctcgatga tcgcgagtgg gctctccgag
     2581 cgcgggttgt ccgacgtgac gatcgcccga tcggcgtacc gaacggcggc agcacccatc
     2641 tccgggcgct tgccacggtc ccggtctcca cccgctccga agactgcgat gacgcggccg
     2701 tccccgagat cgcgagccgc gcgaagtacc cgctcgagcg agtccggcgt gtgtgcgtag
     2761 tcgacgacga cggtgaaggg ctggccctcg tcgacggcct cgagtcggcc ggggatcccg
     2821 ctcactgcgg ccacacccgc agcgatcgcc tggtcgggga cgtcgagcag gaggccacac
     2881 gcaaccgccg cgagcacgtt ctcgacgttg aaggcaccga ggagcggggt ctccagctcg
     2941 atcccggccg cccggaacct gctgccgccc gcccccagct cgaggtcgtc gggggcgacc
     3001 tccgcctctt gggtggcgcc gaacgtgacg agaggggcgc gatgagcgtc gcggagctcg
     3061 tcggcgaggc gtcggccgta cgcgtccgcg acgttcaccg cggccggtgc ggggtgggcc
     3121 cccacgaaca tgcgcctctt tgcctggtag tagtcctcca tcgtctcgtg caggtcgagg
     3181 tgatcctgcg agagattcgt gaagaccagg gcgtcgaagc gaacgcggtc taggcgccgg
     3241 tacgcggagc cgtgcgaaga ggcctcgacg gcgactgcgt cgtcgccgcg ttcgaccatc
     3301 tcgcggaaca gcccctgcag ctcgattgcc tccggcgtgg tgaagggcgc atcacgcagc
     3361 tcgccgccga cgacccagga cacggtgccg acgagtccgc accgccggcc ggcatgttga
     3421 aggatcgagc gcaggagaaa cgccgtcgtc gtcttgccat tcgtgccggt gactccggcg
     3481 acctggagct cccgcgtcgg ctcgccgaag aacacgtccg ctgcgacggc catcgcgctg
     3541 cgaacgtctt cgacgacaag ctgcggaacg gcgacatcga gcggacgctc cacgacgagc
     3601 gcgctggcgc cggcgttgac cgcgtctaca gcgtaggcgt gcccgtccgc gcgcgcacca
     3661 ggcacgcaga agaacagcgt ccccgcagag acacgccgcg cgtcatgagc aagatcgcgg
     3721 atctcagttc ggccaccgcc gatcacgtct gcgggcgcca gcgccccgat caggacgtcg
     3781 agctccaccg acggaaggct agatgactcg ccggaaacgc agcggcctgc ccttcccgca
     3841 ggagccgctc agccgctgct gtacccgcca agtgcctcca acgcggcctc ttccttcgtc
     3901 tccggcgcat cgggaggcac ctccaggtac tgaaggtcga aacgtgcgat ctcggcgaag
     3961 accggggccg cgacgacgcc gccccagatc gtccctttcg gctcgtcgat cgcgaccagg
     4021 acgacgagcc gcgggtcctc cgctggaacg aggccgacga acgaggcgac gtacttcgaa
     4081 acgtagacgc cgttctcggg tttcgctgcg gttcccgtct tcccggcaac ggtgtagccg
     4141 gggatcgccg ccgaggttcc cgtgccctcg accacgacgt cgcgcaacat cgccatgacc
     4201 tggtccgcgg ttgccttcga cacgatccgg cgtcccttgc cgggcttcac ccgcttgccg
     4261 ccgatcttgt cgacgacgtg cggccgctcg cggacgccgc cgttgccgat cgtcgcatac
     4321 gccgctgcca tctgaacggg agtgacggca atgccctgcc cgatcggcac cgtgccgatc
     4381 gtcgagcctg accagtcgtc gagtgggagc accattcccg ggctctctcc ggggaggtcg
     4441 acgcccgtct tgctcccgaa gccgtaccgg tcgatccagg atgccagcgt cccctcgccg
     4501 agcttctggc cgatcgtcac cgtgccgacg ttcgacgagt cggacaggat ctgcttcacg
     4561 gtcatcgacg tcgacccgcg tgcgtgcgac tcgcggatga cgcggtcggc gatgttgatc
     4621 gtcggctgca gccagaacga cgtctgcggg gtgacgatct tctcctcgag cgcggccgag
     4681 atcgtgacga tcttgaaggt cgagcccggc tcgtagacgt cggtcaccgc gcggttgcgc
     4741 cgtcgctcgg ccgacgtcgc ggggaacaga ttcgcgtcga aggtcggcgc gttcgccatc
     4801 gcgaggatcg cgccggtccg cggatccatc acgatcgccg tgccaccctt cgcggcccag
     4861 cgctccacgt gccgagagag cacctgctcc gcattggcct ggatctgatg gtcgatggtc
     4921 aagacgacgt tctggccctg acgctccggt cgcccactga cgacgtcgat agcccggccg
     4981 aacggatcct tcacgaccac ttcgaagccg ggccgaccgg cgagcacatt gtccagcgat
     5041 cgctccaggc cttcgagccc cttgttgtcg agccccgcgt agccgagcac cgtggaggcg
     5101 acctcacgct gagggtacga gcgcagttcc tctgcgtaga agccgagacc ctcgtgctcc
     5161 tgcttctcca gggcggcggc cttgtacggg tcggctttcc gtgccacgta gacgaacccg
     5221 cgcgacttgt cggtgatctc ctggtagagg tcgtcggcgc tgagcccaaa ggccttcgca
     5281 gccgcccgcg cggtccgctg cggattggcg accgaccgtg gatcggcgta gatggtcgtc
     5341 gcctgctcgc cgatcgcgag cggctccccg gtgcggtcgt agatggttcc acgcgcagcc
     5401 ggtatctcga tcgtctcgcg atgctgtcga tcggccatcg tctcgaagct cggtccgcgg
     5461 acgacctgca gccacgccgc ccgtccgagc atgacggcga atacgaggac gaaggcga
//
LOCUS       DBNGNK010000012         7329 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_646037,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000012 DBNGNK010000000
VERSION     DBNGNK010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_646037"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="map"
                     /locus_tag="AC150V_00275"
     CDS             complement(<1..713)
                     /gene="map"
                     /locus_tag="AC150V_00275"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR3761391.1"
                     /translation="MIIRKGPEEIERIARAGALVAATIEHVAGHLEPGISTAELDKVA
                     EDYIVEQGGTPTSRDYKGYPAAICISPNDLIVHGIPGPYRVSEGDLVTIDVGVTYGGA
                     IGDSAFTFGVGDIEPEAQRLLDVCREALAAGIAQARSGNRIGDISHAVQQIVENAGFS
                     VVRSLVGHGVGRHYHEDPHVPNFGEPGRGPRLSEGMTIAIEPMITAGADDVVLHDDGW
                     SISTADRSLAAHFEHTVAIT"
     gene            complement(710..1297)
                     /locus_tag="AC150V_00280"
     CDS             complement(710..1297)
                     /locus_tag="AC150V_00280"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR3761392.1"
                     /translation="MNVLVLGPQGSGKGTQAARIAAEFGVPHVSTGDMFRAAVTEGTE
                     LGREVEPILAAGELVPDELTVELIRARLDRPDAADGFVLDGFPRNLAQAEALDGMLSE
                     IGRPAEVVLFFDLSDEVAVERMQGRAAQEGRTDDTPEVIARRLAIYHEQTAPVVEHYR
                     VTGNLVPLHAERTINEVWAEIQSALQHVESRAVRE"
     gene            complement(1310..2563)
                     /gene="secY"
                     /locus_tag="AC150V_00285"
     CDS             complement(1310..2563)
                     /gene="secY"
                     /locus_tag="AC150V_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR3761393.1"
                     /translation="MLNWLANAWRVPDLRKRVLFTAFILAVYRLGSWIPAPGVDSSAI
                     ENYFSGRGGTIFGLLNLFSGGALSQFSVFALGIMPYITASIILQLMTVVIPKLAELQK
                     EGEAGYAKINQYTRYLTLVLAAAQATGYAILFERQGALETNAGRMVLIIVTLTTGTAL
                     LMWMGEQITKRGIGNGISILIFASILTGLPLGLSAWWNGGPMERIFFPLIGLAIVAAV
                     VFVQEGQRRIPIQYAKRMVGRRMTSGGSTYLPLRVNMAGVIPVIFAAAVLAFPQTVAS
                     YMPGTADFVNEHFQPTDLTYLFVEAVLIIVFAFFYTAVQFNPVDQADNLRRYGGYIPG
                     IRPGPPTAQYLDRVLTRLTFPGALFLASVAIAPAVFIRYGGFSQASAGALGGTSVLIA
                     VGVALDTMRQMESQMMMRNYEGFLR"
     gene            complement(2760..3221)
                     /gene="rplO"
                     /locus_tag="AC150V_00290"
                     /pseudo
     CDS             complement(2760..3221)
                     /gene="rplO"
                     /locus_tag="AC150V_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013637829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(3224..3409)
                     /gene="rpmD"
                     /locus_tag="AC150V_00295"
     CDS             complement(3224..3409)
                     /gene="rpmD"
                     /locus_tag="AC150V_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR3761394.1"
                     /translation="MSTVKITQVRSTIGQTKAHEGTLRALGLGRIGKTAEHEETPQLV
                     GQLRRVRHLVQVEETES"
     gene            complement(3562..4044)
                     /gene="rpsE"
                     /locus_tag="AC150V_00300"
                     /pseudo
     CDS             complement(3562..4044)
                     /gene="rpsE"
                     /locus_tag="AC150V_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            complement(4056..4409)
                     /gene="rplR"
                     /locus_tag="AC150V_00305"
     CDS             complement(4056..4409)
                     /gene="rplR"
                     /locus_tag="AC150V_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR3761395.1"
                     /translation="MSKLSTRDARARRHRRIRGKISGTAERPRLVVYRSNRGIFAQLV
                     DDESGRTLASADWLGLDDFKGDKTEQAAAVGKALGAAAKEAGVTTCVFDRGGYLYHGR
                     VKALAEGAREEGLVF"
     gene            complement(4406..4939)
                     /gene="rplF"
                     /locus_tag="AC150V_00310"
     CDS             complement(4406..4939)
                     /gene="rplF"
                     /locus_tag="AC150V_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR3761396.1"
                     /translation="MSRIGRKPIEVPSTVSVAISPGRVQVNGPLGELTQTVPDRMQIE
                     QEDGVITVTRPSERGQDRALHGLTRTLIANMIEGVTQGFEKHLEIQGVGYRAQLKGQD
                     LELSVGFSHSVTVTPRQGITFELPQPTEVVVKGIDKQVVGQMAAEIRKVRPPEPYKGK
                     GIRYRDEYVRRKVGKRA"
     gene            complement(4953..5348)
                     /gene="rpsH"
                     /locus_tag="AC150V_00315"
     CDS             complement(4953..5348)
                     /gene="rpsH"
                     /locus_tag="AC150V_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007937701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR3761397.1"
                     /translation="MLTDPIADMLSRVRNANRAMHDSVAMPTSRMKEEIARLLKEEGY
                     IKDYRVVAGEPYASLVIELKFGRNRERVLTDLKRVSKPGRRVYARKDRLPRVLGGLGT
                     SILSTSTGVITGRQAAERGVGGEVIAFVW"
     gene            complement(5374..5559)
                     /locus_tag="AC150V_00320"
     CDS             complement(5374..5559)
                     /locus_tag="AC150V_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR3761398.1"
                     /translation="MAKKSLVAKQKRASKYKTRAYSRCNRCGRPRAVYRKFGLCRICL
                     RELAHQGAIPGMTKSSW"
     gene            complement(5562..6140)
                     /gene="rplE"
                     /locus_tag="AC150V_00325"
     CDS             complement(5562..6140)
                     /gene="rplE"
                     /locus_tag="AC150V_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR3761399.1"
                     /translation="MSVTTENYVPRLKERYVDEIRPQLMAELGLDSIMQVPRVTKVTL
                     NMGVGDAKTEAKALEAAIEELTTIAGQRAQQRLARKSIASFKLREGMPVGARVTLRGA
                     RMYEFIDRLVSIALPRIRDFRGLNPRSFDGRGNYSIGIREQVIFPEIDYDSVASIRGL
                     DIAITTSAASDEHAFALLRALGLPFAAERRDN"
     gene            complement(6137..6508)
                     /gene="rplX"
                     /locus_tag="AC150V_00330"
     CDS             complement(6137..6508)
                     /gene="rplX"
                     /locus_tag="AC150V_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR3761400.1"
                     /translation="MATTMKVKKGDLVHVLSGKDRGKQGRVIEARPREGRVLVENLNL
                     VKRNMRPRPMRDAGRMGGTQIIPGGIIEKPSPLDVSNVMVVCPVCNVPTRVGIHVKDV
                     KGETVRVRVCKREGCGQELDR"
     gene            complement(6513..6881)
                     /gene="rplN"
                     /locus_tag="AC150V_00335"
     CDS             complement(6513..6881)
                     /gene="rplN"
                     /locus_tag="AC150V_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003940951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR3761401.1"
                     /translation="MIQQESRVRVADNTGARELLCIRVVGGSRRRYARVGDVIVATVK
                     AATPQGTVKKGEVVKAVVVRTKKPYGRDDGTLIGFDENAAVIIDAQNNPRGTRIFGPV
                     ARELRERNFMKIVSLAPEVL"
     gene            complement(6901..7170)
                     /gene="rpsQ"
                     /locus_tag="AC150V_00340"
     CDS             complement(6901..7170)
                     /gene="rpsQ"
                     /locus_tag="AC150V_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR3761402.1"
                     /translation="MPKPESERGRRQERQGTVVSAAMEKTIVVEVDMLKAHRMYKKVL
                     KRSTRFHAHDEENSAKVGDVVRIVETRPLSKQKRWRLVEIVQEAK"
BASE COUNT         1087 a         2530 c         2407 g         1305 t
ORIGIN      
        1 gtgatcgcga cggtgtgctc gaagtgcgcg gcgagtgacc ggtcggcggt cgagatcgac
       61 cagccgtcgt cgtgcaggac gacgtcgtcg gctcccgcgg tgatcatcgg ctcgatcgcg
      121 atcgtcatcc cctccgagag gcgtggtcca cgacccggct cgccgaagtt ggggacatgg
      181 ggatcctcgt ggtagtgacg accgacgccg tggccgacga ggctgcgaac gacggagaag
      241 cctgcgttct cgacgatctg ctgcaccgca tgagagatgt cgccgatgcg attgccggac
      301 ctggcctggg cgattcccgc cgcgagcgcc tcgcgacaga cgtcgagcag gcgctgcgcc
      361 tcgggctcga tatcgccgac accgaaggtg aaggcgctgt cgccgatggc gccgccgtag
      421 gtgacgccga cgtcgatcgt gacgagatct ccctcggaga cgcggtacgg gccagggatg
      481 ccgtgaacga tgaggtcgtt cggcgagatg cagatcgcag cgggataccc cttgtagtcc
      541 ctcgaggtcg gagtgccacc ctgctcgacg atgtagtcct cggcgacctt gtcgagctcc
      601 gcggtgctga ttcccggctc gaggtggccg gccacgtgct cgatcgtcgc tgcgaccagc
      661 gcgcccgcgc gggcgatgcg ctcgatctcc tcgggcccct tccggatgat cattcgcgca
      721 ccgctctcga ctcgacatgc tgcagggcgg actggatctc ggcccagacc tcgttgatcg
      781 ttcgctcggc gtgcagcggg acgaggttcc ccgtgacgcg gtagtgctcg acgaccggcg
      841 ccgtctgctc gtggtagatc gcaagcctgc gggcgatcac ctccggcgtg tcgtccgtgc
      901 gcccctcctg cgcggcgcgc ccctgcatcc gttcgacggc gacctcgtcc gagaggtcga
      961 agaagagcac gacctcagca gggcgcccga tctcggacag catcccgtcg agcgcctccg
     1021 cttgcgccag gttccgagga aatccgtcga ggacgaaccc gtcggcggca tcgggccggt
     1081 cgagccgcgc tcggataagc tcgacggtca gctcgtccgg gacgagctcg cctgcggcca
     1141 ggatcggctc cacttcacgg ccgagctcgg tgccctcggt gacggcggcg cgaaacatgt
     1201 cgccggtcga gacgtgcggt acgccgaact cggccgcgat gcgcgccgct tgggttccct
     1261 tcccggagcc ctgtggtccg agcacaagga cgttcacggc gtcgcgcagc tacctcagga
     1321 agccttcgta gttgcgcatc atcatctgcg actccatctg ccgcatcgtg tccagggcga
     1381 cgccgacggc gatgagcacg gaggtaccgc ccagcgcacc ggcactggcc tgcgagaacc
     1441 cgccgtagcg aatgaacacc gcgggagcga tcgcgacgga ggcgaggaac agcgcgcccg
     1501 ggaaggtcag tcgcgtcagg acgcggtcga ggtactgcgc ggtcggcggg ccgggacgta
     1561 tgcccgggat gtagccgccg taccgacgca ggttgtcggc ctggtcgacc gggttgaact
     1621 gcaccgccgt gtagaagaac gcgaagacga tgatcaggac cgcctcgacg aagaggtagg
     1681 tgaggtcggt gggctggaag tgctcgttga cgaagtcggc cgttccaggc atgtacgacg
     1741 ccacggtctg cgggaaggcg aggacggcgg cggcgaagat gaccgggatg acgccggcca
     1801 tgttgacgcg cagggggagg taagtcgatc cgcccgaggt catgcggcgg cccaccatgc
     1861 gtttcgcgta ctggatcggg atgcggcgtt gcccctcctg gacgaagacg accgcggcga
     1921 cgatcgcgag cccgatcagc gggaagaaga tgcgctccat cgggccgccg ttccaccagg
     1981 cgctgagacc gagcgggagg ccggtcagga tggaggcgaa gatcaggatc gagatgccgt
     2041 tgccgatgcc gcgcttcgtg atctgctcgc ccatccacat caggagggcc gtgccggtcg
     2101 tcagcgtgac gatgatcagc accatgcgcc cggcgttcgt ctcgagcgca ccctgccgct
     2161 cgaacaggat cgcgtacccg gtcgcctgcg cggccgcgag caccagagtg aggtagcgcg
     2221 tgtactggtt gatcttggcg tagccggcct cgccctcctt ttgcagctcg gcgagcttcg
     2281 ggatcacgac cgtcatgagc tggaggatga tcgaggccgt gatgtacggc atgatcccga
     2341 gcgcgaaaac ggagaactgg gacagcgccc cacccgagaa gaggttgagc aggccgaaga
     2401 tcgtgccgcc gcgaccgctg aagtagttct cgatcgcgga ggagtcgacg cctggagcgg
     2461 ggatccagga ccccagccgg tagacggcca ggatgaaggc cgtgaagagg acgcgcttcc
     2521 ggaggtcagg aacgcgccag gcgttggcga gccagttcag catggccttc gctcagtgtg
     2581 cctgcggtcc cgtcctgtag acaggcctaa ccggcctcct ccgcggccac cggctcgtcg
     2641 gcctcggcct ccgactccgg ttcgacatcg ccttccggct ccgagtcggc gctcggttgt
     2701 gcaggtcgcc gcgactttcg cttcttcggc acgggctcgc cccgcagcca gacaatcgtg
     2761 cccccggccg cctcgatctt cgcccgggca gacttcgaga agccgtgcac tgcgacggtg
     2821 agagacttgg tgagctcgcc ctcgccgagg atcttcacgt cgacgcgagt gttcttcagc
     2881 agcccctttt cgaccagggc ctcgggggtc acctcggagc ccgcgtcgaa gcgagcctcg
     2941 agatcgcgga cgttgacggg ctggctgtaa gtccggaacg gcccgaccgg catcgcgtcg
     3001 gccgatgtgt tgcctcgtag cttcggcagc cgcatgtcga tcggcatctg cccgccctcg
     3061 aacccggccg gcagcttgtg ggagccggcg cgcgacttct gccccttgat cccgcggccc
     3121 gaatagcgac ccttgcccga gcccataccc cgcccgacgc gcttgcgcga tcgccgcggc
     3181 tgggccggct tgagcgagtg gagattgagc tcgtcggaca ccgctaggac tccgtctctt
     3241 cgacctggac gagatggcgc acacgcctca gctggccgac gagctggggc gtctcctcat
     3301 gctcggcagt ttttccgatt cgcccaagcc ccaatgcgcg caaggtgccc tcgtgcgcct
     3361 tcgtctgccc gatggtgctt cgcacctgcg tgatcttcac ggtgctcacc cggatgcctc
     3421 ctcgaccgga gcctcgtctg tcggttcaac gggagcgctc tccgccggct cggcggactc
     3481 gagaggctcc gactgcgcag cgcgcgccgc cgtggcctcg gcctcggcgg cctccggctc
     3541 ctcgctcggc ggcacgtacg gcagcacttc cctgatcgtc ttgccgcgcg cctttgcgac
     3601 gtcctgcggg ctgcgcaggc tctgcaggcc gttgacggtc gccttcgcca tgttgatcgg
     3661 attcgtcgta ccgagactct ttgccagcac atcgcggatg ccggcgagct cgagcacggc
     3721 gcgcacgccg ccgccggcga tcacgcctgt cccctcgctg gcgggacgca ggaacacgcg
     3781 cgccgcgtcc caacgcccga ggatctcatg ggtgatcgtc gagccgtgct tcggcacggt
     3841 gaagatgttc ttcttcgcgt cgtcgacggc cttggagatc gcgagcggca cctcgcgagc
     3901 cttcccgtag cctactccga cgcgatcgac ctcgtcgccg atcgcgacca gcgccgtgaa
     3961 cgagaagcgc cggccgccct tgaccacctt ggcgacgcgg ttgatctcga tcacgcgctc
     4021 cttgagctcc ctcgtctccg aaatcccggt ggtactcaaa agaccagtcc ttcctcgcgc
     4081 gcgccctcag cgagcgcctt gacgcgtccg tggtagaggt agccgccgcg gtcgaacaca
     4141 caggtcgtga ccccggcctc ctttgccgcg gcgccgagcg ccttgccgac ggcggcggcc
     4201 tgctcggtct tgtccccttt gaagtcgtcc agcccgagcc agtcggccga agcaagtgtg
     4261 cgaccactct cgtcgtcgac gagctgggcg aagatccccc gattcgagcg gtagaccacg
     4321 agcctggggc gctcggcggt tccggagatc ttgccgcgaa tgcgccggtg gcgtcgcgcg
     4381 cgtgcgtcgc gtgtcgagag cttgctcatg cgcgcttgcc cacctttctc cgcacgtact
     4441 cgtcgcggta gcgaatgcct ttccccttgt agggctcggg cggccgcacc ttgcggatct
     4501 cggcggccat ctgccccaca acctgcttgt cgatgccctt gacgacgacc tcggtcggct
     4561 gcggcagctc gaaggtgatg ccctgtcggg gcgtcaccgt caccgaatgc gagaagccga
     4621 cggagagctc cagatcctgt cccttgagtt gcgcccgata cccgacgccc tggatctcga
     4681 ggtgtttctc gaatccctgc gtcacaccct cgatcatgtt cgcgatcaac gtgcgagtga
     4741 gcccgtgcag cgcccgatcc tgaccccgct cggacggtcg cgtgacggtg atgacgccat
     4801 cctcctgctc gatctgcatg cggtcgggga cggtctgcgt cagctcaccg agcgggccgt
     4861 tgacctgtac gcgcccaggc gagatggcga cgctcaccgt cgacggcacc tcgatcggct
     4921 tgcgcccgat ccgactcatt gcctccgctc ctctaccaca cgaacgcgat cacctcgccg
     4981 cccacgccgc gctccgcggc ctggcgaccc gtgatcaccc cggtcgaggt tgacaggatg
     5041 gaagtgccca gccctccgag cacgcgcggc aggcgatcct tgcgcgcgta cacccgtcgg
     5101 cccggcttgg agacccgctt caggtcggtg agcacgcgct cccgattacg cccgaacttg
     5161 agctcgatca cgagcgacgc gtagggctcg cccgcgacga cccggtagtc cttgatgtag
     5221 ccctcctcct tgagcaggcg ggcgatctct tccttcatcc gtgaggtcgg catcgccacg
     5281 gagtcgtgca tcgcacgatt cgcgttccgg acgcgggaga gcatgtctgc gattggatcg
     5341 gtcagcatct ttcgtcctcc ggtagcccgc cgctcaccag ctgctcttcg tcatgccggg
     5401 aatagccccc tggtgtgcga gttcacgtag gcagatccgg cacaggccga acttccgata
     5461 gaccgcccgc ggacggccgc agcggttgca gcgcgaatag gcgcgcgtct tgtacttcga
     5521 ggcgcgcttc tgcttcgcga cgagcgactt cttggccatg cctagttgtc ccttctctca
     5581 gcggcgaacg gcagtccgag tgcacgaagc aacgcgaagg cgtgctcgtc gctcgctgcc
     5641 gacgtcgtga tcgctatgtc gaggccccgg atcgacgcca cgctgtcgta gtcgatctcc
     5701 ggaaagatca cctgctcgcg gatcccaatc gagtagttgc cacgtccgtc gaacgagcgc
     5761 ggattgagtc cgcggaagtc gcggatgcgc ggcagcgcaa tcgaaacgag gcggtcgatg
     5821 aactcgtaca tgcgggcgcc ccgtaacgtg acgcgggcac ccacgggcat gccctcgcgc
     5881 agcttgaagc tcgcgatcga cttacgggcc aggcgctgtt gggcgcgctg gccggcgatc
     5941 gtcgtcaact cctcgatcgc cgcctcgagc gctttcgcct cggtcttggc gtcgccgacg
     6001 cccatgttga gcgtcacctt cgtcacccgc ggcacctgca tgatcgagtc gagccccagc
     6061 tcagccatca gctgcggacg gatctcgtcc acgtagcgct ctttcaagcg cggaacgtag
     6121 ttctcggtcg tcacgctcac cggtcgagct cctggccgca gccctctcgc ttgcacacgc
     6181 ggacgcgaac cgtctcgcct ttgacgtcct tgacgtggat cccgacccgc gtcggcacgt
     6241 tgcagacggg gcagaccacc atcacgttcg agacgtcgag gggggacggc ttctcgatga
     6301 tgccgcccgg gatgatctgc gtgccgccca tccggccggc gtcgcgcatc ggccgcggcc
     6361 gcatgttgcg cttgacaagg ttcaggttct ccacgagcac cctgccctcg cggggccgcg
     6421 cctcgatcac ccgtccctgc ttaccgcgat cctttcctga caggacgtgg acgaggtctc
     6481 ccttttttac cttcatcgtt gtggccatct cgctaaagca cctctggggc aaggctgacg
     6541 atcttcatga agttcctctc gcgcagctca cgggcgacgg gcccgaagat acgcgtaccg
     6601 cgcgggttgt tctgggcgtc gatgatgacg gccgcgttct cgtcgaagcc gatcagtgtt
     6661 ccgtcgtctc tcccgtaggg cttcttcgtc cgaacgacga cggccttgac gacctcgcct
     6721 ttcttcacgg tgccctgcgg ggttgcggct ttcacggtag caacgatgac gtcaccgacc
     6781 cgcgcgtaac gccgccggga gccgccgacg actcggatgc agagaagctc gcgcgcgccg
     6841 gtgttgtcgg caacgcggac gcgtgactcc tgctggatca cggacggagg cccccctgtg
     6901 tcacttcgcc tcctggacga tctcgacgag acgccagcgc ttctgcttcg acagcgggcg
     6961 ggtctcgacg attcggacga catcgccgac cttggcgctg ttctcctcgt cgtgcgcgtg
     7021 gaaccgagtc gatcgcttga gcaccttctt gtacatccgg tgcgccttga gcatgtcgac
     7081 ctcgaccacg atggtcttct ccatcgcggc ggagacgaca gtgccttgcc gctcctggcg
     7141 ccggccccgc tcggactccg gcttcggcag gcgaacgatc ggcttccgcg tctcgggctt
     7201 gatccgcttg cgcttcgggc gctggcggcg ctgcgggcgc ggcgggcgct gcctcttggt
     7261 cttcttcgcg accgcggccg cctcggcagc cgccttctcg tcgccgtcgt ccgccgcggc
     7321 gggctcggc
//
LOCUS       DBNGNK010000013         3276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_287869,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000013 DBNGNK010000000
VERSION     DBNGNK010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_287869"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1380)
                     /locus_tag="AC150V_00345"
     CDS             complement(1..1380)
                     /locus_tag="AC150V_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3761403.1"
                     /translation="MTVGQRSEEGVLRGKRGLPAGLAVVATALIAAGCGGGEDAQSDR
                     VEAVPISSCTPIEYGGEGKPDLLVVSDFPHQGPSRARVAQIVEAIRYELDLRGWIAGS
                     HRIGFQSCDHSTPEARDWDPGKCTENADAFVTNDKVVGVIGPFNSPCAAVEIPVLNEA
                     AGGGVAMISPATTYTCLAKAGPGCEEIEPEKYYPAGERNFTRVIGNDSIQGAALAELM
                     KQRGVKRLFILFDGRRYGTVVATATRKAAGALGIAVVGNKAWDPAASSASYEALFREV
                     GKSGADAVLLAGVFEENGAEMIKAKVAVLGPNAGRVKLYGPHGFAAQGTIDETGGAAE
                     GMVVAVAGVPLDALGAKGKAYAAKFQAARLTGGAVDPYAVYGAQAARIMLNAIGKSNG
                     TRAGVVRQLFRTKVDDGLIGTFAFDEDGDPVDATGPVVGFTVLEAADTFEPDGVVMPT
                     PETVRRAVG"
     gene            1652..2515
                     /locus_tag="AC150V_00350"
     CDS             1652..2515
                     /locus_tag="AC150V_00350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3761404.1"
                     /translation="MKPASFHYVAPTTLAETLSLLGEHGGDGSLLAGGQSLVPLMNLR
                     LARPAVVIDVGRVTALRGVANGDGVRIGAATTQARVLAEPSVLSAAPLLCSALREVGT
                     PATRARGTVGGSLAHADPAAELPAAMLALDAEVAVASSSGARTIGASDLFRGPFTTSL
                     APDELLTEIRIPPGAATRRYGFAELARRHGDFALAGACCTATVSEGLFQEVRVALFGV
                     GPTPIRVQEAETALEGAVCDDPSAIAAAAELAAGACEPSGDVHASAGYRRRAAGVVVR
                     RAIAQMGGMDG"
     gene            2508..2996
                     /locus_tag="AC150V_00355"
     CDS             2508..2996
                     /locus_tag="AC150V_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3761405.1"
                     /translation="MVERKPVTIRVNGTALSGTAEPRTLLSDFLRHELDLIGTRVACE
                     QGGCGACTVLIEGTLALSCLTFAVQADGTSVETVEGLSQGGRLHPIQLAFQQEHGLQC
                     GYCTPGMLMATKALLDANPAPSEEEIREHLGGNVCRCTGYAGIIAAVKAAAAGPAREA
                     NG"
     gene            2993..>3276
                     /locus_tag="AC150V_00360"
     CDS             2993..>3276
                     /locus_tag="AC150V_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003890601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3761406.1"
                     /translation="MSAESETTVPTRYVGARMLRVEDPRLLAGRAEFVDDVRRPALHH
                     AVFVRSPYAHARVGAIETSAAAAVDGVAAVLTGHDLAEVPSIATTIATRP"
BASE COUNT          446 a         1184 c         1141 g          505 t
ORIGIN      
        1 tcagccgacg gcgcgccgta cggtctccgg cgtgggcatg accacgccgt cgggctcgaa
       61 cgtgtccgcc gcctcgagca ccgtgaagcc gacgacgggg cccgtcgcat cgacggggtc
      121 gccgtcctcg tcgaacgcga acgtcccgat caggccgtcg tcgaccttcg tcctgaagag
      181 ctggcgcacg acgccggccc gtgttccgtt ggacttcccg atcgcgttga gcatgatccg
      241 cgcggcctgc gctccgtaga cggcgtacgg gtcgaccgcg ccgccggtga ggcgcgcggc
      301 ctggaacttg gccgcgtacg cctttccctt cgcgcccagg gcgtcgagag gcacgcctgc
      361 tacggccacg accatgcctt cggccgcacc tcccgtctcg tcgatcgtgc cctgcgcggc
      421 gaagccgtgc ggtccgtaaa gcttcaccct gcctgcgttc ggcccgagca cggcgacctt
      481 cgccttgatc atctcggcgc cgttctcctc gaagacgccg gcgagcagca cggcgtcggc
      541 cccggacttg ccgacctcgc ggaaaagcgc ctcgtaggag gcggacgagg cggccggatc
      601 ccaggctttg ttgccgacga cggcgatccc gagcgccccg gcggctttcc gtgtcgccgt
      661 cgcgacgacc gttccgtaac gcctgccgtc gaacaagatg aagagccttt tgacgccccg
      721 ctgcttcatg agctccgcga gcgcggctcc ctgaatgctg tcgttgccga tcacgcgcgt
      781 gaagttgcgc tcccctgccg ggtagtactt ctcgggctcg atctcctcgc agccgggtcc
      841 ggctttcgcg agacacgtgt aggtcgtggc gggcgagatc atcgccaccc cacctccggc
      901 cgcctcgttg agcaccggga tctcgaccgc cgcgcacggt gagttgaacg gaccgatcac
      961 gccgacgacc ttgtcgttcg tcacgaacgc gtccgcattc tccgtgcact tgcccggatc
     1021 ccagtcgcgc gcctccgggg tcgagtggtc gcacgactgg aagccgatcc ggtgcgagcc
     1081 ggcgatccag ccgcggaggt cgagctcata gcgaatcgcc tcgacgatct gcgccacccg
     1141 tgcgcgcgac ggcccctggt gggggaagtc ggagaccacg aggagatcgg gcttgccctc
     1201 gccgccgtac tcgatcggcg tgcacgacga gatcggcacg gcctccacac ggtcgctctg
     1261 cgcgtcctcg ccgccgccgc accccgcggc aatgagcgca gtcgcgacga ctgcgaggcc
     1321 ggccggcaac cctctcttgc ccctcagcac tccctcctcg ctccgctgac caacggtcat
     1381 cggcgggaca cgctgggccc gtgtccggct ccctccgaac cgtcgctgga cggtagcgga
     1441 ggctcccctc ccgccgcaac atgcacgtga aaccgacggg cgatacgccc tcgctgagat
     1501 aggctgacgg cgccgcgccc cggcagccca tggtccactg gcatttcacc ttcaggcggt
     1561 gtccgcatgc tgatcactgc gctgccggcc ccgatcagta cgcacctgct gctgggcggg
     1621 ctcgagggct tccacgcgtg gcgggccgtc ggtgaagcct gcgtcctttc attacgtcgc
     1681 gccgaccacg ctcgccgaaa ccctctcgct gctcggcgag cacggcggtg acgggtcgct
     1741 cctggccggc gggcagagcc tcgtcccgtt gatgaacctg cggttggcgc gcccggccgt
     1801 cgtgatcgac gtgggacgag tgaccgcgct ccgaggtgtc gccaacggcg acggcgtgcg
     1861 gatcggcgcc gccacgactc aggcccgcgt cctggcggag ccgtcggtgc tgtccgccgc
     1921 acccttgctg tgcagtgccc tgcgcgaggt cggcacgccg gcaacgcggg cgcgcggaac
     1981 ggtgggcggc tcgctcgctc acgccgatcc ggccgccgag cttccggcgg cgatgctcgc
     2041 cctcgacgcc gaggtcgcgg ttgcgagcag ctcgggggca cgcacgatcg gcgcgtctga
     2101 tctcttccgc ggccctttca ccacgtctct tgcccccgac gagctgctga ccgagatccg
     2161 catcccgccg ggcgcggcga cccgacggta cggcttcgcc gagctcgcac gccgccacgg
     2221 ggacttcgcg ctcgcaggag cgtgctgcac ggccacggtg agcgaaggct tgtttcagga
     2281 ggtacgggtc gctctcttcg gggtcggccc gacgccgatc cgagtgcagg aggccgagac
     2341 ggcactcgag ggggcggtgt gcgacgaccc gagcgcgatc gcggcggcgg ccgagctcgc
     2401 agccggagcg tgcgagccgt ccggcgacgt gcatgcctcg gccggctaca ggcggcgggc
     2461 ggcgggcgtc gtcgtcagac gggcgatcgc acagatgggc ggcatggatg gttgagcgaa
     2521 agccggtcac gattcgcgtg aacggcacgg cgctctcggg gacggccgag ccgcgcacgc
     2581 tgctctcgga cttcctgcgt catgagctcg atctcatcgg gacgagggtc gcctgcgagc
     2641 agggtggctg cggcgcctgc accgttctga tcgagggcac gctggcgctc tcctgcctga
     2701 ccttcgcggt tcaggccgac ggcacctcgg tcgagaccgt cgaggggctc tcgcaaggcg
     2761 gccgcctcca tccgatccag ctcgccttcc agcaggagca cggactgcag tgcgggtact
     2821 gcacgccggg aatgctgatg gcgacgaaag cgctcctgga cgcgaatccc gccccctccg
     2881 aggaagagat ccgcgagcat ctgggcggga acgtgtgccg ctgcacgggc tacgcgggaa
     2941 tcatcgccgc cgtcaaagcg gccgcggcgg ggcctgcacg ggaggcgaac ggatgagcgc
     3001 ggagagcgag acgacggtgc cgactcgcta cgtcggcgcg cgcatgctgc gggtcgaaga
     3061 tccccggttg ctcgccggca gggccgagtt cgtcgacgac gtgcggcggc ccgcgttgca
     3121 ccacgccgtg ttcgtgcgga gtccgtatgc gcacgctcgc gtgggcgcga tcgagacgtc
     3181 ggccgccgct gcggtggacg gcgtcgccgc cgtgctgacg ggacacgatc tcgcggaggt
     3241 tccctcgatc gcgacgacga tcgccacccg gccgaa
//
LOCUS       DBNGNK010000014         3510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_717865,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000014 DBNGNK010000000
VERSION     DBNGNK010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_717865"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="AC150V_00365"
     CDS             complement(<1..286)
                     /locus_tag="AC150V_00365"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761407.1"
                     /translation="MAPGGDGRRTPVHCPGRRGARLPQRAARTSTDPRALREHVAGRL
                     SGKACVITGAGGGMGREAAQVFTREGAKVCVADISQAAAAETVELCPPESA"
     gene            complement(178..918)
                     /locus_tag="AC150V_00370"
     CDS             complement(178..918)
                     /locus_tag="AC150V_00370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3761408.1"
                     /translation="MPVRPLIGITAYAQPARFGAWDLPAALIPLMYVDAVEAAGGRAL
                     VVPPTDVGLEETLDCLDGLVFSGGADLDPRRYGAEPHPQTDSPQERRDRAELALLAAA
                     LARDMPVLGICRGSQLLNVAHGGDLDQHLPDTVGNTNHRETPGVFSEHIVDVREGTRL
                     AETVGVRAKVTSSHHQGLGRVGAGLIEAAWAEDGTLEAIEDPQRRFAVGVLWHPEAME
                     DGRLFTALVVEARAYRSERRGRPPTRER"
     gene            1691..2833
                     /locus_tag="AC150V_00375"
     CDS             1691..2833
                     /locus_tag="AC150V_00375"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3761409.1"
                     /translation="MRRLIVLVSAVIFTDTVLFGAIIPLVPSFADDFDLSKLEAGVLV
                     GAYGAGALVGGIPGGLLARRIGPRATVTAGLLVLAAASVAFALAGDPLALGLSRFGQG
                     MASVVTWSGALAWVVAGTPRERRGEILGTVFAFAVLGFIVGPIVGAVAELIGIRATFA
                     IVAGVVLVLAGVAALAADAPPGELLPGALRRAASDPVFLVGLWLALLPALLFGVIDVL
                     VPLALDAAGWGAFAVAAVFVVAGLAETGVNPLIGRLSDRRGRFVPIRWALAAAAVIGL
                     GLAFAETPLLIAGLIVAASVATGGFYTPGMALVSDRAELAGLAQGLAFGFLNTAWAAG
                     AMIGPTLGGALADGLGDAAPYALGAALAAVTLLIVSSSPVTRTRAV"
     gene            2830..3426
                     /locus_tag="AC150V_00380"
     CDS             2830..3426
                     /locus_tag="AC150V_00380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3761410.1"
                     /translation="MKRERWNERYASTDLLWSAGPNRFLAAETADLSPGRALDLACGE
                     GRSALWLAELGWQVTAVDFAEVGLEKGKRLAEQRGVEVDFVHADLLDWTPPEQAFDLV
                     VVLYLQVPAEDRRSILASAASAVAPGGTLLLVGHDSTNLTDGAGGPPDPRVLYTPEDV
                     VADLPGLEIEKAERVLRPVEGADRPAIDALVRARRPPA"
BASE COUNT          487 a         1202 c         1227 g          594 t
ORIGIN      
        1 aggccgactc gggcgggcag agctccaccg tctccgcagc ggccgcctgt gagatgtcgg
       61 ccacgcacac cttggcgcct tcacgtgtga acacctgggc tgcctcacgc cccatgccgc
      121 cgcccgcgcc ggtgatgacg cacgccttgc cgctgagtcg tccggccacg tgctctctca
      181 gcgctcgcgg gtcggtggac gtccgcgccg ctcgctgcgg taggcgcgcg cctcgacgac
      241 cagggcagtg aacaggcgtc cgtcttccat cgcctccggg tgccacagta cgccgaccgc
      301 gaagcgccgc tgcggatcct cgatcgcctc cagcgtcccg tcctcggccc aggcggcctc
      361 gatcagccct gcgccgacac ggcccaggcc ctgatggtgc gacgacgtga cctttgctcg
      421 cactcccacg gtctccgcga gccgcgtgcc ctcgcggacg tcgacgatgt gctcggagaa
      481 gacgccaggg gtctcgcgat gattggtgtt gcccacggtg tcggggagat gctggtcgag
      541 atcgccgccg tgggcgacgt tcaggagctg gctgccgcgg cagatcccga gcacgggcat
      601 gtcacgcgcg agcgccgccg cgagcagcgc caactccgct cggtcgcgcc gctcctgcgg
      661 cgagtcggtc tgcggatggg gctcggcccc gtaccggcgc gggtcgaggt cggcgccgcc
      721 ggagaacacc agcccgtcga ggcagtcgag cgtctcctcg agaccgacgt ctgtcggcgg
      781 cacgaccagg gcacggcccc cggccgcctc gactgcatcg acgtacatca gggggatcag
      841 cgcggcgggc aagtcccagg cgccgaagcg ggcgggctga gcgtaggcgg tgatcccgat
      901 cagcggtcgt acgggcacgg cggaaagcgt acgccgtccg gcggaggggt gtcccgcggg
      961 ctcggcaccg cgagtgactc ctcgaacgtc cgcaatttgc gcgaacttcg tccccgcaag
     1021 gccttgatcg gccgtggcca gtctgtagag tcgcttttgc gccgagcacg gggcgtgagt
     1081 aggacgaagc ttcgccggga ggcactacgc ggaagcggaa ggtcgaccgg ggtggtggac
     1141 gaaagcgcaa ccggctcggc gaacttttgc ccggaaatcg acccgggttt caggcgccct
     1201 ttttagccga gtagctcacg tatcagagac ggtcgtttcg caacgcgatc tgaccatcct
     1261 gaaaaatccg caaattgcgg gcgggagttg cgtgttggaa cttgaccgcg accacgacgc
     1321 gctttagtgt cgccgatgca ccgagtacgg agcgccggag gttgagggcg atcccagcgg
     1381 cagtcgcttc ggcggatgtc ggtcggatca gtcggacgaa ggcgtaaccg gctcggtgct
     1441 ttttctgtgg aattacctcc tgaaattgca atttgcaagg aaaacatgat tggcgcaatt
     1501 tgcgaataat ttgcgacaac atgaccttga cgagacgttg agcgatccgt aacgtcccgg
     1561 atgcaccgag tacggagcgc tggaggatga agggctcccg agcggcagcc gcctcggcgg
     1621 aggtcgatcg agaggccgga cgaaagcgtc accggctcgg tgcgtttttg tgcacgcgta
     1681 tgctcgcgcg gtgcgacgcc tgatcgttct cgtctctgcc gtgatcttca cggacaccgt
     1741 cctcttcggg gcgatcatcc cgctcgttcc gtcgttcgcc gacgacttcg atctctcgaa
     1801 gctggaagcg ggcgtgctcg tgggtgccta cggtgccgga gcgctcgtcg gcggcatccc
     1861 cgggggcctt ctcgcgcgac gcattggccc acgggccacg gtgaccgcgg gcctcctcgt
     1921 gcttgctgct gcgagcgtcg cattcgccct ggccggcgac ccgctcgcgc tcggcctctc
     1981 tcgcttcggg cagggcatgg cgagtgtcgt cacctggtcc ggtgccctcg cgtgggtcgt
     2041 cgctggcacc ccgcgcgagc gtcgcggcga gatactcgga acggtctttg ctttcgcggt
     2101 gctcggcttc atcgtcggcc cgatcgtggg cgcggtcgcc gagctcatcg gcatccgcgc
     2161 cacctttgcg atcgtcgccg gggtcgtcct cgtcctcgcg ggcgtcgcag cgctcgcagc
     2221 ggacgcccca cccggcgagc ttctgccggg tgctctccgc cgggcggcat cggacccggt
     2281 gttcctcgta gggctctggc tggctctgct gcccgctctg ctcttcggcg tcatcgatgt
     2341 gctcgttccg ctcgcgctcg atgccgcggg ttggggcgcc tttgccgtgg cggctgtctt
     2401 cgtcgtcgca gggctcgccg agaccggtgt gaacccactc atcggccgtc tctcggatcg
     2461 gcggggccgc ttcgtcccga tccgttgggc tctcgccgcc gcggccgtca tcgggttggg
     2521 actggcgttt gccgagacgc cgctgctcat cgcggggctc atcgtggccg cgtcagtcgc
     2581 cacgggcggc ttctacacgc ccgggatggc gctcgtctcg gaccgggcgg agctcgccgg
     2641 gctggcgcag ggcctcgcat tcgggttcct caacaccgcg tgggccgcgg gggcaatgat
     2701 cgggccgacg ctcggcggcg cgttggccga cgggctcgga gacgcggccc cctacgccct
     2761 cggagcggca ctcgcggcgg ttacgctcct gatcgtcagc tcctccccgg tgaccagaac
     2821 gagagccgta tgaaacgcga gcgctggaac gaacggtacg caagcaccga cctcctctgg
     2881 agcgccgggc cgaaccgatt cctcgcagcc gagacagccg acctgagccc cgggagggcc
     2941 ctcgacctcg cctgcggcga gggtcgcagc gctctctggc tcgccgagct cggctggcag
     3001 gtcaccgcgg tcgacttcgc cgaggtcgga ctggagaaag ggaaaaggct ggcggagcaa
     3061 cgcggcgtcg aggtcgattt cgtccacgcc gatctgctcg actggacgcc ccccgagcag
     3121 gcgttcgacc tggtcgtcgt cctctacctc caggtaccgg ccgaggatcg tcgatcgatc
     3181 ctcgcgagcg cggcgtcggc cgtcgcaccg ggcggcacgc ttctgctggt cggccacgac
     3241 tcgacgaatc tcaccgacgg agcgggaggc ccgcccgatc ccagagtgct ctacacgccc
     3301 gaggacgtcg tcgccgacct cccggggctt gagatcgaga aagcagagcg agtgctccgg
     3361 cccgtcgagg gagccgaccg gcccgcgatc gacgctctcg ttcgggctag gcggccgcca
     3421 gcctgaggtc tgggggaaag cgctcgagag cccagtcggc gtcgagcggc cgcttgcggt
     3481 agtcgcagcc gttgacgagc acgaagtccc
//
LOCUS       DBNGNK010000015         3949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1721377,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000015 DBNGNK010000000
VERSION     DBNGNK010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1721377"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1419
                     /gene="aceA"
                     /locus_tag="AC150V_00385"
     CDS             115..1419
                     /gene="aceA"
                     /locus_tag="AC150V_00385"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR3761411.1"
                     /translation="MTPAPATPTPAEETARLAAAWDGDERWAGLARDYTPEDVVRLRG
                     SVQVEHTLARRGAERLWRTLHTDDYVNALGAMTGGQAVQMARAGLRAIYVSGWQVAAD
                     GNLSGSVYPDQSLYPANSVPAMVRRINNALLRADRIDWAEGVRERDWLLPIVADAEAG
                     FGGPLNGFELMTQMIEAGAAGVHFEDQLSSEKKCGHLGGKVCVPTGHFIRMLSAARLA
                     ADVAGVPTVLIARTDADSAKLLTSDVDERDRPFLTGERTAEGFFRVTGGIDSAIARGL
                     AYAPYADLVWMETSTPDLAEARAFADAMHAEFPGKLLAYNCSPSFNWKKHLDEQTIAA
                     FQRELGAIGFCFQFITLAGFHSLNASMFELARGYAERGMSAYVELQEHEFALEPSGYS
                     ATRHQREVGAGWFDEIAQAVSGGSSSTLALEGSTEEAQFAAA"
     gene            1604..2266
                     /locus_tag="AC150V_00390"
     CDS             1604..2266
                     /locus_tag="AC150V_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014296784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HIR3761412.1"
                     /translation="MTEIAPEPMQLTVVENGRHAPRLVEVKSAAVEVKDRLANPAPLG
                     LMAFGLTTILLNVHNAGFTQLDTMILAMGVFYGGIAQVIAGLIEFRKDNVFGATAFCS
                     YGLFWLSLVGLLVFPELGMGDKPTAGAMGAYLFVWGLFTALMFVGSLRTNRALQAVFA
                     ALAILFFLLAVGEWTGSDTVLKVAGWEGIVTGALAVYAAIAQVWNEMYGRIVLPLGPT
                     GR"
     gene            2499..>3949
                     /locus_tag="AC150V_00395"
     CDS             2499..>3949
                     /locus_tag="AC150V_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3761413.1"
                     /translation="MAVTAGVAGDARVGTELAGYSIEALLGRGGMGVVYLARDPWLER
                     KVALKVLLPELSERGRFRERFLRESKLAASLEHANVVPIYEAGDAQGLLYLAMRYVEG
                     PDLGTLLGRSGRLNPKRALAILEQVAIALDAAHAKGLVHRDVKPGNVLLSLDEGDGER
                     AYLSDFGLSKTANEGTDFTEAGQLAGTLRYMAPEQVQEAEVSAQTDIYALGCVLFECL
                     TGAAPFAHETVMALLWAHLEEDPPSIAEQARGLPATLDGVIAKALAKEPADRYETCGQ
                     LIAAAAGELGEAGAARRASRNPYKGLRAFDEADADDFFGRDALVGELLERLAHPERGR
                     LVALVGSSGSGKSSVLKAGLLPRLAQGAIPGSEDWTVLEALPGDPISDLEAGIEALRD
                     LASPRLVVVIDQFEELFTMVADESRREAFVSLLVRTAERADGNVVLTLRADFYDRPLR
                     YRELSRLLERGLVTVHPLEPEELERAIVAPAERV"
BASE COUNT          611 a         1322 c         1394 g          622 t
ORIGIN      
        1 cagaaaacag gattcagatg tttggatccg ataaaggcac aagaactctc ttgacggcat
       61 gaaaacccgt atctaggatc cgaagacttt tacttcggat cccagaggag ctctatgacg
      121 cccgcacccg ccaccccgac gcctgccgag gagaccgccc gtctcgccgc cgcctgggac
      181 ggcgacgagc gctgggccgg tctcgcccgc gactacacgc cggaggacgt cgtgcgtctg
      241 cgcggttcgg tgcaggtgga gcacaccctg gcgcgccgcg gcgctgagcg gctctggcgc
      301 acgctccata ccgacgacta cgtcaacgcg ctcggcgcga tgacgggcgg gcaggcggtg
      361 cagatggcca gggccggcct gcgggcgatc tacgtgtccg gctggcaggt ggccgcggac
      421 ggaaacctga gcggctccgt ctacccggat cagagcctct acccggccaa cagcgtgcct
      481 gcgatggtgc gccgcatcaa caacgctctc ttgcgggccg accggatcga ctgggccgag
      541 ggcgtgcgtg agcgcgactg gctgctcccg atcgtcgccg acgcggaggc cggcttcggc
      601 ggaccgctca acggcttcga actgatgacg cagatgatcg aggcgggcgc cgccggggtg
      661 cacttcgaag atcagctgtc gtcggagaag aagtgtggcc acctgggcgg caaggtctgc
      721 gtgccgaccg gccacttcat ccgcatgctg agcgccgcgc ggctcgccgc cgacgtcgcg
      781 ggcgtgccca cggtgctgat cgcacgcacc gacgccgact cggcgaagct gctcacgagc
      841 gacgtcgacg agcgcgatcg ccccttcctc acaggcgagc gcaccgccga gggtttcttc
      901 cgcgtcaccg gcgggatcga ctccgcgatc gcgcgcggac ttgcctacgc gccctacgcc
      961 gacctcgtct ggatggagac ttccacgccc gacctcgccg aggcgcgtgc gttcgccgac
     1021 gcgatgcacg ccgagttccc gggcaagctg ctcgcctaca actgctcgcc gtccttcaac
     1081 tggaagaaac acctcgacga gcagaccatc gccgcgttcc agcgcgagct gggcgcgata
     1141 ggcttctgct tccagttcat cacgctcgcc ggcttccatt cgctcaacgc ctcgatgttc
     1201 gagcttgcgc gcggctacgc ggagcgcggt atgagcgcgt acgtcgagct ccaggagcac
     1261 gagttcgcgc tcgagccgag cggctactcg gcaacgcgtc atcagcgcga ggtgggtgcc
     1321 ggctggttcg acgagatcgc ccaggccgtg tccggcggct ccagctcgac gctggcactc
     1381 gagggctcga ccgaagaagc acagttcgcg gctgcgtagg tgacaagagc agtcgtgtga
     1441 gcggggccgc ggtgggggtc gcggcggctc tgctccgtct cgtcggtgaa tccacctacc
     1501 cgaccacggg ccgaaaccgc agtcagcacc gccgccgatc cgcaacgccc cggatgccgg
     1561 cgctgcccgt gctccttacg gtgcaggtga aagggtgatt cggatgaccg agatcgcacc
     1621 agagccgatg cagctgacgg tcgtcgagaa cggccgacac gcaccgcggc tggtcgaggt
     1681 gaagagcgcc gccgtcgagg tgaaggatcg cctcgcgaat ccggcgccgc tcggactgat
     1741 ggcgttcggc ctgacgacga tcctgctcaa cgtccacaac gccggcttca cgcagctgga
     1801 cacgatgatt ctggcgatgg gggtcttcta cggcggcatc gcccaggtga tcgccggctt
     1861 gatcgagttc cgcaaggaca acgtcttcgg cgccaccgcg ttctgctcat acgggctgtt
     1921 ctggctctcg ctggtcgggc tgctcgtgtt ccccgagttg ggcatggggg acaagccgac
     1981 ggcgggcgca atgggggcgt acctgttcgt ctggggcctg ttcaccgccc tgatgttcgt
     2041 cggctcgctc cgcacgaacc gcgccctgca ggcagtgttc gcagcgctgg cgatcctctt
     2101 cttcctgctc gcggtcggcg agtggaccgg cagtgacacg gtgctgaagg tcgccggctg
     2161 ggagggcatc gtcaccggcg cgctcgccgt ctacgccgcg atcgcgcaag tgtggaacga
     2221 gatgtacggg cggatcgtgc tgccgctcgg gcctactggc cgctaacaac tcggcccgct
     2281 tctcaaacgg ttgtgcgaca tgcgacaatt gctgtcgctg tcttgattgg caacgcgtca
     2341 aaggaggggt acctcgagcg cgcggcggca accgtgaaga caaccgcggt tggtaggcgg
     2401 tcggtaagcg cgtggtcagc gccgccgtgc acgttgacgg ccatggcgca cgcgcgacgc
     2461 ctgtgctccg cctccgcgag gtcggagcgg tagccggcat ggccgtcaca gcaggcgtcg
     2521 ctggcgacgc tcgagtcgga accgagctcg ccggctactc gatcgaggcg ctcctcgggc
     2581 gcggcggtat gggtgtcgtc tacctggccc gcgacccctg gctggagcgg aaggtggcgc
     2641 tcaaagtgct cctgcccgag ctgtcggaac gtgggcgctt ccgggagcgg ttcctgcgcg
     2701 agtcgaagct ggcggcgtcg ctcgagcacg cgaacgtcgt accgatctat gaggcgggcg
     2761 acgcccaggg gctgctgtac ctggcgatgc gctacgtcga gggacccgac ctgggcacat
     2821 tactgggccg cagcggaagg ctaaatccga agcgcgctct cgcgatcctg gagcaggtgg
     2881 cgattgcact cgacgcggcg cacgcgaagg gcctcgtgca tcgcgacgtg aagcccggca
     2941 acgtcctgct ctcgctcgac gagggagacg gggagcgcgc ctacctctcg gacttcggcc
     3001 tctcgaagac cgcgaacgag ggaaccgact tcactgaggc gggccagctc gccgggacgt
     3061 tgcgctacat ggcgccggag caggtgcagg aagccgaggt gtcggcccag accgacatct
     3121 acgcgctcgg ctgtgtgctc ttcgagtgcc tcacgggcgc agctcccttc gcgcacgaga
     3181 cggtgatggc cctgctatgg gcgcacctcg aagaggaccc gccgagcatc gcggaacagg
     3241 cgcgaggcct cccagcaacc ctcgacgggg tgatcgcgaa ggcgctggcg aaggagcccg
     3301 ccgaccgcta cgagacctgc gggcagctga tcgcagcggc cgccggggag ctgggcgagg
     3361 cgggcgccgc gcggcgcgcg tcccgcaacc cgtacaaggg gctgcgcgcg ttcgacgagg
     3421 ccgacgctga cgacttcttc ggccgcgatg cgctggtcgg cgagctcctc gaacgccttg
     3481 cacatccgga gcggggtcgg ctggtcgcgc tcgtcggctc gtcgggctcg ggcaagtcga
     3541 gcgtgctcaa ggcgggcctg ctgcctcgac ttgcccaggg agcgatcccg gggtcagagg
     3601 actggacggt cctggaggcg ctgccgggtg acccgatctc cgacctcgag gccgggatcg
     3661 aggcgctgcg cgacctggcc agcccgcggc tcgtcgtcgt gatcgaccag ttcgaggagc
     3721 tgttcacgat ggtcgcggac gagtcgcgtc gagaggcgtt cgtatcgttg ctcgtccgga
     3781 ccgccgagcg ggcggacggg aacgtcgtcc tgacgctccg cgccgacttc tacgaccggc
     3841 cgctgcgcta cagggagctc agccggttgc tcgagcgcgg tctcgtcacc gtgcatccgc
     3901 tcgagcccga ggagctcgag cgcgcgatcg tcgcgccggc cgagcgggt
//
LOCUS       DBNGNK010000016         3557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1578575,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000016 DBNGNK010000000
VERSION     DBNGNK010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1578575"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="AC150V_00400"
     CDS             <1..624
                     /locus_tag="AC150V_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR3761414.1"
                     /translation="DRGALVLADSYQAVGAIDLDVRELAVDFVTGGTVKYLLGSAGLG
                     FLYVRGELLEQLLPTQTGWFADEDIFQMDISDYSPATDARRFDAGTPPVPNIYGGLAG
                     LSIVEEAGVATVEAHVRGLNERLLAGLDELGAVVVTPREQEQRGPLICVRSTDVDALV
                     QRLAEEQIVVSSRDGNVRISAHIYNVDGDVDRVLDAFSRNRALLASR"
     gene            complement(628..1392)
                     /gene="arsM"
                     /locus_tag="AC150V_00405"
     CDS             complement(628..1392)
                     /gene="arsM"
                     /locus_tag="AC150V_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020575152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR3761415.1"
                     /translation="MTTTDAASVREFVRERYAEAARSATEGSSCGCGCGPSCCSEEGA
                     ATFGASLYAITDRNALPDAAVRASLGCGNPASVADMAEGETVLDLGSGGGIDVILSAR
                     RVGPTGMAYGLDMTDEMLDLARANAAEAGVENARFLKGRIEDVPLPGASVDVVISNCV
                     VNLSPEKDAVLGEIMRVLKPGGRVGITDIVAEDRLSQSERAERGSYAGCIAGALSRGE
                     YEQGLSSIGFEDVSVTFTHAVADGMHSAIIRATKPT"
     gene            complement(1389..1730)
                     /locus_tag="AC150V_00410"
     CDS             complement(1389..1730)
                     /locus_tag="AC150V_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3761416.1"
                     /translation="MSVDLIGSECCRPLDDDGMTKADARATAALFKALSDPARVRIVN
                     LLATRESPACVCQLTGPLGLSQPTVSHHLKKLVKAGRLEREQRGTWAYYSLDRDAFRQ
                     LASVTEMEGVR"
     gene            1810..2700
                     /locus_tag="AC150V_00415"
     CDS             1810..2700
                     /locus_tag="AC150V_00415"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3761417.1"
                     /translation="MPPRVHLTDAVPAPVLDEIDRDFEQLETLAGADGLVAMTTARVD
                     GDFLDAAGPQLRIVANYGAGIDNVDLDAARERRVVVTNTPDVLTHATAELAITLMLSL
                     LRRVTEGDRFVRSREPWRFSLEFMLGEGIDGKVLAVVGPGRIGRETARLAVALGMRVL
                     LVGRNDDLAAALAEADVVSLHCPLTPETRHLIGDEALSAMKPTAVLVNTARGAVVDEA
                     ALSRALHAGRIAGAALDVFEHEPDVTESLLALENVILSPHLGSATRETRIAMGMLCVE
                     ALRAVLLEDRVPANAVVVPG"
     gene            complement(2697..3143)
                     /locus_tag="AC150V_00420"
     CDS             complement(2697..3143)
                     /locus_tag="AC150V_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3761418.1"
                     /translation="MPRQPRSLLPDGPYHVMSRGVEQRAVFHDQSDRHDFLGLLRDVT
                     ASWEWSFHALCLMTSHYHLVFESTRAGLSDGMRRLNGVYAQRFNIRHGRWGHLFGDRF
                     ACRPIESEEHLRAVCRYVLLNPVEAGLCERAADWPWSYSRYDFGDD"
     gene            3293..>3557
                     /locus_tag="AC150V_00425"
     CDS             3293..>3557
                     /locus_tag="AC150V_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761419.1"
                     /translation="MERIVVAAVGDSITAGSPYWDPDPAVRSRIGEELDPESQWELWA
                     QRLDPRLEFRNHGVYGERAEEIAARFEGALDSASAVVVQGGIND"
BASE COUNT          551 a         1208 c         1215 g          583 t
ORIGIN      
        1 gaccgtgggg cgctcgttct cgccgacagc taccaggccg tgggcgcgat cgatctcgac
       61 gtgcgcgagc tggccgtcga cttcgtcacg ggcgggacgg tcaagtacct cctgggctcc
      121 gccggtcttg gcttcctgta cgtacgcggc gagttgctcg agcaactcct gccgacgcaa
      181 accggctggt ttgcagacga ggacatattc cagatggaca tctccgacta ctcgccggcg
      241 accgacgcgc gccgcttcga cgcgggaacg cccccggtgc cgaacatcta cggcgggctg
      301 gccgggctct cgatcgtgga ggaggcggga gtcgccaccg tcgaggcgca cgtccgtggc
      361 ctcaacgagc gcctgctggc cgggctcgac gagctcggcg ccgtcgtggt cacgccgcga
      421 gagcaggagc agcgcggccc gttgatctgc gtccgctcga ccgacgtcga cgcgctggtg
      481 caacgactcg cagaggagca gatcgtcgtc tcgtcccgcg acggcaacgt ccggatctcc
      541 gcgcatatct acaacgtcga cggcgacgtc gatcgcgtgc tcgacgcgtt ctcccgaaac
      601 cgcgccctgc tcgcctcccg ctagtggcta ggtgggcttg gtcgcgcgga tgatcgcgct
      661 gtgcatgccg tcggcaaccg catgcgtgaa ggtgacgctc acgtcctcga atccgatcga
      721 gctcaggccc tgctcgtact cgccgcgcga gagcgctccg gcgatgcagc cggcgtagct
      781 cccccgctcg gctcgctcgg attgcgagag gcggtcttcg gcgacgatgt cggtgatgcc
      841 gacgcgcccg ccgggcttca ggacgcgcat gatctcgccg agaacggcgt ccttctccgg
      901 tgacagattg acgacgcagt tcgagatgac gacgtcgacg ctcgcccccg gcagggggac
      961 gtcctcgatg cgacccttca gaaagcgggc gttctccacg cctgcctcgg ccgcattcgc
     1021 gcgagccagg tcgagcatct cgtccgtcat gtccagcccg taggccattc cggtcgggcc
     1081 gacccggcgg gctgagagga tcacgtcgat ccccccgccc gagccgaggt cgagaaccgt
     1141 ctcgccctcg gccatgtcgg cgaccgatgc ggggttcccg cagccgaggc tggcgcggac
     1201 ggcggcgtcc ggcagagcgt tccggtcggt aatcgcgtag agcgacgctc cgaaggtagc
     1261 ggcgccctct tccgagcagc acgagggccc gcagccacaa ccgcaggagc ttccctcggt
     1321 cgcactacga gcagcctccg cgtagcgctc gcgaacgaac tcgcgaaccg aggctgcatc
     1381 agtcgtggtc atctgactcc ctccatctcc gtcaccgacg cgagctgccg gaacgcatct
     1441 cggtcgaggc tgtagtaggc ccaggtgccg cgctgctctc gctcgagccg cccggccttc
     1501 accagcttct tcaggtggtg gctgaccgtc ggctgcgaca agccgagcgg gccggtcagc
     1561 tggcagacgc atgccgggct ctctcgcgtc gccagcaagt tgacgatgcg cacgcgcgcg
     1621 ggatcggaca gcgctttgaa gagcgccgcc gtcgcacggg cgtcagcctt cgtcatcccg
     1681 tcgtcgtcga gggggcgaca gcattcgctc ccgattagat cgacactcat cgatgcagag
     1741 tatggcaact agatcgatat ttgtcaatca ataggttcga acgtgcgcgg cgtgcgagga
     1801 tcggttgccg tgcccccgcg agtccatctc accgacgccg tgccagcgcc cgtgctcgac
     1861 gagatcgacc gcgacttcga gcagctcgaa acgttggcgg gcgccgacgg tctcgtcgca
     1921 atgacgaccg ctcgtgtcga cggcgacttc ctcgacgcgg cgggtccgca gctgcggatc
     1981 gtcgcgaact acggagccgg catcgacaac gtcgacctgg acgcggcccg cgagcgccga
     2041 gtcgtcgtga cgaacacgcc ggatgtcctc actcacgcca ccgccgagct cgcgatcacc
     2101 ctgatgctgt cgctgctacg gcgggtcacg gaaggcgacc gcttcgtccg ctcgcgcgag
     2161 ccttggcgct tctctctcga gttcatgctc ggagagggga tcgacggcaa ggtgctcgcc
     2221 gtcgtcgggc ccgggcgaat cggccgcgag acggcgagat tggcagtcgc cctcggaatg
     2281 cgggtgctcc tggtgggccg aaacgacgat ctggcggcgg cgctcgccga ggcggacgtg
     2341 gtcagcctgc actgcccgct cacgcccgaa acacgacacc tgatcggtga tgaggcgctg
     2401 tctgcgatga agcccacagc ggtgctcgtc aacaccgccc gaggggccgt cgtcgacgag
     2461 gcggcgctct ctcgggcact ccacgccggt cggatcgcgg gcgccgcgct ggatgtgttc
     2521 gagcacgaac ccgatgtgac ggagtcgctg ctggcgctcg agaacgtcat cctgtctccg
     2581 cacctcggga gtgccacgcg cgagacgcgt atcgcgatgg gcatgctctg tgtcgaggcg
     2641 ttgcgcgccg tgctgctgga ggatcgggtt cccgccaacg cagtcgtcgt ccccggctag
     2701 tcgtcgccga agtcgtagcg gctgtacgac cacggccagt cggcggcacg ctcgcagagg
     2761 ccggcctcga ccggattcag cagtacgtag cgacagactg cgcggaggtg ttcctcgctc
     2821 tcgatcggcc ggcaggcgaa gcggtcgccg aagagatggc cccagcgtcc atgccggatg
     2881 ttgaaccgtt gtgcgtagac accgttcagc cgccgcatac cgtcggagag acctgcgcga
     2941 gtcgattcga acacgaggtg gtagtggctc gtcatcaggc agagggcgtg gaacgaccat
     3001 tcccagctcg ccgtcacatc gcggaggagt ccgaggaagt cgtgtcggtc ggactggtcg
     3061 tggaacacag cccgttgctc gaccccgcgg ctcatgacgt ggtacgggcc gtcggggagg
     3121 agcgagcgcg gttgtcgcgg caccgggtca gggtaggacg cccggctgcg cctgtgcccg
     3181 ctgtggatta ctcggcgcgc ttttggcgca gttcagacac ggccgttccg gccatgtcgg
     3241 gggacagtcc cctgaagtcc gtccgctcct ctgcgccgac taatctcctg ccgtggagcg
     3301 gatcgtcgtc gcggcggtcg gggactcgat cacggccggg tctccgtact gggatcccga
     3361 cccggcagta cgcagccgta tcggcgagga actcgatccg gagagccagt gggagctctg
     3421 ggcgcaacgc ctcgaccccc gactcgagtt ccgcaaccac ggcgtgtacg gcgaacgggc
     3481 agaggagatc gccgctcgct tcgagggggc gctcgacagc gcctctgcgg tcgtcgtcca
     3541 gggcggaatc aacgaca
//
LOCUS       DBNGNK010000017         6982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_862216,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000017 DBNGNK010000000
VERSION     DBNGNK010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_862216"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="AC150V_00430"
     CDS             <1..345
                     /locus_tag="AC150V_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3761420.1"
                     /translation="LLPINSETLYLDLTFITLAMLVIGGAVSLWGAVVGALVVSGLDS
                     LLAQAENGLDVADATIDLPSGTRIVVIGALMALVLIIRPTGITGGREFSLDRFRRRGG
                     HAAPPAAEGGTK"
     gene            342..1271
                     /locus_tag="AC150V_00435"
     CDS             342..1271
                     /locus_tag="AC150V_00435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3761421.1"
                     /translation="MRICVVGCGAVGSLFAANLAQLDDVEVWGYDLNGAHVDAINANG
                     LRLVGAGEVLGFLQATTDAAELPACDLGIVATKAMHADAAIAATAAAFADGAVASVMN
                     GVGNEETLAAHVTRVIRGTTFPAGKLLEPGVVQWDVRGDTTLAPFESSPASADEVERL
                     ADACTRAGMPTSAVADARPAQWRKVIFNAATNPIGALTGLTHGRVCERPDLRALVSAL
                     VVEGKAVAAAQGIVPDADPEELVDHAARPDVAYDHKASMLQDVEVQRPTEIDFLNGGI
                     ARFGREHGVPTPLNDTITALVKGVEASWTSQLS"
     gene            1250..2368
                     /locus_tag="AC150V_00440"
     CDS             1250..2368
                     /locus_tag="AC150V_00440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3761422.1"
                     /translation="MDLAAELARRYGNIRAAMAEHGLDALVVAGSEYTGFEGAVTYLS
                     GFQIVHRYAYVVLPAEGEPVAVFPAEARYVGEHGTALVEQVFAPHPGAEIASRARDGG
                     WRRLGVYGLHYVMAVRDYQALGDLDIVPFDVEFDLARAVKSEAELESVRDSVRINRRG
                     FEIWFEHYEPGKTAAEVMAAAEEYFVAEGCGRLTMNMVLTAPGRSGLAFPEFKIARLE
                     EVLGDFVLPSLEVAGPGMHWVEVSRAVAASGTAPSDDTRQMLEAYVEYYDVASTAIRP
                     GASCHDVHRAVSQGFVDRGYRLGHVTGHSIGMTMIEFPKIGEGVEHELAENMVLSMHP
                     HAIAANGEDCLYMQDTWLVTADGGVPLASLPMELYVKQ"
     gene            2365..2982
                     /locus_tag="AC150V_00445"
     CDS             2365..2982
                     /locus_tag="AC150V_00445"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020048622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR3761423.1"
                     /translation="MTANGIRVGEDGVARCWWGTEPPIYTAYHDDEWGRPVVDDTRLF
                     EKLALEGFQSGLSWLTILRKREGFRRAFAGFDIERVARFGPRDVERLLGDASIVRHRG
                     KIEATIANAERALELREQEGSLAAYVWSFEPEPTTRPARMTREMLLQLTTSPEAVALA
                     KDLKRRGWRFVGPTTVYSFMQAMGLVNDHLDGCAFRDLIESIRSS"
     gene            3039..4346
                     /locus_tag="AC150V_00450"
     CDS             3039..4346
                     /locus_tag="AC150V_00450"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3761424.1"
                     /translation="MRTGTVVKLTGVAVLAIAAMGVTGAGLCKYKLECHLRPPESLDE
                     YAEHGQGRTALPAGFRQRVVASGFSFPTDFAFLPDGSALIAEKEGIVKIAARSGGPAR
                     TVLDLRDRVDSSGFRGLVSVDVDPEFERNHFVYLMYVRQEPDEEEGKPTTVRVSRFGF
                     SGRSIARSSEQVLLGRVSAAPCLRTVRADCLPSDVDHDGGDIAFAEDGTLFVATGDGG
                     GLEKVEATALLAQNIDYLGGKILHVTRDGRGLPGNPFCNGDADANGDVPTVVVDIRLT
                     LGPSDHLPYVADVGWQHAEEISVAPRGANLGWPCYEGRDHTPQYESDRACAKLYGKGA
                     AAVRPPLISLPHPDAASVTGGVFYLGDSYPKQYHGAYFFADWGLSWIRYARPGGSAHA
                     RLADVKPFASNAAGPVAFRIGPDGDLYYLSLNTGQLRHVLYVR"
     gene            4391..4630
                     /locus_tag="AC150V_00455"
     CDS             4391..4630
                     /locus_tag="AC150V_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761425.1"
                     /translation="MGWPWIAVLLLAALVVVAAEWPRLRRVVGADARKKREREKRKAK
                     LRVVNTDDEDFVASVQRDLESLPTIEERETGPSGR"
     gene            complement(4667..>6982)
                     /locus_tag="AC150V_00460"
     CDS             complement(4667..>6982)
                     /locus_tag="AC150V_00460"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3761426.1"
                     /translation="VSFASPPLARSAGDPAAEARLTREHARFERALAQAVPGAEVRWR
                     YRVVANGMAVVVPPASVSTVARLPGVEEIFHGVRYSPQLDRSVLQIQAQKLWNAAVGS
                     AGAGIKIGIIDDGIDQEHPFFDPSGFKLPAGYPKGQRAYATAKVIVARAFPPPNAGWK
                     NAARPFDAELSAHGTHVAGIAAGNADTRTDGNGGRTISGVAPRAYLGNYKALTIPTDA
                     NVGLDGNSPELVAAIEAAVADGMDVINLSIGEPEIEPTRDLVALALDAAAAAGVVPVV
                     AAGNDFGEFGPGSVTSPGSAADAITVSAVTTAGVGGRGVLAGFSSAGPTPLSLRLKPD
                     VAAPGVSILSSEPDGWGTLSGTSMAAPHVAGAAALLRERHPSWTVSQLKSALSVTADP
                     VSRQESAKTEAAPTRAGGGLVNLTRADRPLLFASPTSLSFGLVGRDSEAIAAVSLSDA
                     GGGAGVWNVAVVPNVARAGVGVSAPKDVSVPGDLTVTVAATAAAAEGEAAGYVVLTRG
                     AERRRIPYWLRLAVPALPGHPAKALTRPGTYGGNTRGRQKLVDRYRYPEVPPAGVVSG
                     SLKGPEQVFRLTLKRPVANFGVVVIERARGVAVEPRVVETGDENRLTGYAGLPLNLNP
                     YLVDFGDRVPAAGAVRPKAGSYDIVFDSPGPSGAGAFRFRLWINDTKPPLIRLRTASV
                     AAGTPVVLKVTDGGAGVDPTSIVSEIGELEGKAQLRAGTIRVSTAGLKPGKHRLRVQV
                     SDYQESRNTENVAAILPNTRVLTTTVTIVSK"
BASE COUNT         1092 a         2330 c         2456 g         1104 t
ORIGIN      
        1 ctgctgccca tcaacagcga gaccctgtac ctcgatctga cgttcatcac actcgcgatg
       61 ctcgtgattg gaggcgccgt cagcctctgg ggcgcggtcg tcggggcgct cgtcgtgagc
      121 ggtctcgact ccctgctcgc acaggccgag aacgggctgg acgtcgccga tgcgacaatc
      181 gacctgccct cggggacgcg catcgtcgtc atcggagcgc tcatggcgct tgtcctcatc
      241 atccgtccga ccggcatcac gggtgggagg gagttctcgc tcgaccgatt ccgtcgccgc
      301 ggcggtcacg ccgctccgcc cgcagccgag ggcgggacga aatgaggatc tgtgtcgtcg
      361 gctgtggcgc tgtcgggtcg ctgttcgcgg cgaacctcgc ccagctggac gacgtcgagg
      421 tgtggggcta cgacctgaac ggcgcacatg tggacgccat caacgcgaac gggttgcggc
      481 tggtcggcgc cggggaggtt ctcggctttc tgcaggcgac cacggatgcc gcggagctcc
      541 ccgcctgcga cctcgggatc gtcgcgacga aggcgatgca cgcggacgct gcgatcgccg
      601 ccacagccgc tgctttcgcc gacggtgcgg tcgcatcggt catgaacggc gtcggaaacg
      661 aggagactct cgccgcccac gtcacgcgcg tgatccgcgg cacgaccttt ccggccggga
      721 agctgctcga gccgggcgtc gtgcagtggg acgtgagggg cgacacgacg cttgcgccgt
      781 tcgagtcgag ccccgcgtcc gcggacgagg tcgagcgcct ggccgacgcg tgcacgcgcg
      841 cgggcatgcc gacgagcgcc gtcgccgacg ctcgaccggc ccagtggcgg aaggtgatct
      901 tcaacgccgc cacgaatccg atcggcgccc tcaccggcct gacgcacggt cgcgtatgtg
      961 agcggcccga tctgcgcgct ctcgtctcgg ctctggtggt ggaaggcaag gcggtcgcag
     1021 ctgcgcaggg gatcgtcccg gacgccgacc cggaggagct cgtcgatcac gccgccaggc
     1081 cggatgtggc ctacgaccac aaggcgagca tgcttcaaga cgtcgaggtg caacgaccga
     1141 cggagatcga cttcctcaac ggcgggattg cacgcttcgg tcgcgaacac ggcgttccga
     1201 cgccgctcaa cgacaccatt acagcgctcg taaagggggt agaggcatcg tggacctcgc
     1261 agctgagcta gccaggcgct acggcaacat ccgcgccgcg atggccgagc acgggcttga
     1321 cgcgctcgtc gtcgcgggga gcgagtacac cgggttcgag ggggccgtga cgtacctctc
     1381 cggcttccag atcgtccatc gctacgcgta cgtcgtgctg ccggcggaag gcgagcccgt
     1441 cgcagtcttc ccggcggagg cacggtacgt cggcgagcac ggcaccgcgc tggtcgagca
     1501 ggtgttcgct ccccaccccg gcgcggagat tgcgtcgcgg gcacgcgacg gcggctggcg
     1561 ccgcctgggc gtgtacgggc tccactacgt gatggcggtc cgcgactacc aagcgcttgg
     1621 cgacctcgac atcgtgccct tcgacgtcga gttcgacctc gcacgcgcag tgaagtccga
     1681 ggccgagctc gagtcggtcc gcgactccgt gcgcatcaac cggcgcggct tcgagatctg
     1741 gttcgagcac tacgagccgg gtaagaccgc ggcggaggtg atggccgccg ctgaggagta
     1801 cttcgtcgcc gaaggctgcg ggcggctcac gatgaacatg gtgctgacgg cgcccggtcg
     1861 cagcgggctc gccttcccgg agttcaagat cgcccggctc gaggaggtgc tgggagactt
     1921 cgtgctgccc tcgctcgagg tcgccgggcc gggcatgcac tgggtggagg tgtcgcgcgc
     1981 cgtggcggcg agcgggacgg ctccgtcaga cgacaccagg cagatgctcg aggcgtacgt
     2041 cgagtactac gacgtcgcct cgaccgccat acgcccggga gcgtcgtgcc acgacgtcca
     2101 ccgggcagtg tcccaaggct tcgtcgatcg tggctaccgc ctcgggcacg tcacgggcca
     2161 ctcgatcggc atgacgatga tcgagttccc caagatcggc gagggtgtgg agcacgagct
     2221 ggccgagaac atggtgctct cgatgcaccc gcacgcgatc gccgcgaacg gtgaggactg
     2281 tctctacatg caggacacgt ggcttgtcac cgcggacggc ggcgtgccgt tggcctccct
     2341 ccccatggag ctgtacgtga agcagtgaca gcgaacggaa tcagggtcgg tgaggacggc
     2401 gtcgcccgct gctggtgggg cacggagccg cccatctaca ccgcgtacca cgacgacgag
     2461 tggggaaggc ccgtcgttga cgacacgagg ctgttcgaga agctcgcgct cgaaggcttt
     2521 cagtcggggc tgagctggct gacgattctt cgcaagcggg agggctttcg tcgggcgttc
     2581 gccggcttcg acatcgagcg ggtggcgcga ttcgggccgc gagacgtcga gcggctgctg
     2641 ggcgatgcct cgatcgtgcg ccaccgcggc aagatcgagg cgactatcgc caacgcggag
     2701 cgggcactcg agctgcgcga gcaagaagga tccctcgccg cgtacgtgtg gagcttcgag
     2761 cccgagccga cgacccgccc cgctcgaatg acaagggaga tgcttctgca gctcaccact
     2821 tcccccgagg ccgtcgcgct cgccaaggat ctgaagcgac gcggctggcg cttcgtcgga
     2881 ccgacgacgg tctactcctt catgcaggca atggggctgg tcaacgacca cctcgacggg
     2941 tgcgcgtttc gcgacctgat cgagtcgatt cggagctcct gaggcttcaa cgcgaggact
     3001 actggacagc cgcccagcgc gcacgcatga tgcgaggcgt gagaacaggg acagtcgtca
     3061 agctgacggg tgtcgctgtt ctcgcaattg ccgccatggg ggtgaccggc gcagggctgt
     3121 gcaagtacaa gctcgaatgc catctccgcc ccccggagtc tctcgacgag tacgcggagc
     3181 atggacaggg gcgcacggct ctccccgccg gcttccgcca gcgagtggtc gcatccggct
     3241 tcagctttcc gaccgacttc gccttcctcc ccgacggctc ggccctgatt gccgagaagg
     3301 aagggatcgt gaagatcgct gcccgatcgg gcgggcccgc tcgcactgtt ctcgatctcc
     3361 gcgatcgtgt ggattcgagc gggttccgcg gactcgtctc cgtcgacgtc gacccggagt
     3421 ttgagcgcaa ccacttcgtc tacctcatgt acgtcaggca agagcccgac gaggaagagg
     3481 ggaagccgac aaccgtgcgt gtgagtcgat tcgggttctc cggtcgcagc atcgcaagat
     3541 cgagcgagca ggtactgctc ggtcgcgtct ccgcggctcc gtgcctcagg acggtaaggg
     3601 cggactgcct tccctctgat gtcgaccacg acggtgggga catcgccttc gccgaggacg
     3661 gcaccctctt cgttgcgacc ggagacggcg gggggctcga gaaggtcgag gcgaccgccc
     3721 tgctcgcgca aaacatcgac tatctgggcg gcaagatcct gcacgtgacg cgcgacggcc
     3781 gaggactgcc ggggaatccg ttctgcaacg gcgacgcaga cgcgaacggc gatgtcccca
     3841 ccgtcgtggt cgacatcaga ctgacgctgg ggccgagcga ccatctcccg tacgtcgccg
     3901 acgtcggatg gcagcacgca gaggagatct cggtggcgcc ccgtggggca aacctcggct
     3961 ggccgtgtta cgaggggcgt gaccacacac ctcagtacga gagcgaccga gcctgcgcga
     4021 agctgtatgg aaagggggcc gcggcggtcc ggccgccgct catctcgctg cctcatccgg
     4081 atgcggcgtc cgtgaccgga ggtgtcttct acctcggcga cagctatccg aagcagtatc
     4141 acggggcgta cttcttcgcg gactggggcc tctcgtggat ccgctacgca cgccccggtg
     4201 ggagcgcgca cgcgcgtctt gcagacgtga agccgttcgc atccaacgct gccgggcccg
     4261 tcgcgtttcg catcggcccg gacggcgatc tctactacct gtcgctcaac accggccaat
     4321 tgaggcacgt gctctacgtt cgctgaggac gtgggcctgc gggcgacgcg cggcacgcta
     4381 gaatcgagcc gtgggctggc cgtggatcgc cgtgctcctg ctcgcggctc tcgtcgtggt
     4441 cgcggcggaa tggccgcgcc ttcggcgcgt cgtcggagcg gacgcacgga agaagcgaga
     4501 gcgtgagaag cgcaaggcga agcttcgcgt cgtgaacacg gacgacgagg acttcgtcgc
     4561 cagcgtgcag cgcgatctcg agtcgctacc gaccatcgaa gagcgcgaga ccggcccgag
     4621 cggtcgctga ccgagccagc cacgagccac ggcgcatcgg agtccgctat ttgctcacga
     4681 tcgtgacggt cgtggtgagc acgcgcgtgt tcggcaggat ggccgcaacg ttttccgtgt
     4741 tccggctctc ctgatagtcg gagacctgca cccggagccg gtgtttgccc ggtttcaatc
     4801 ctgcggtcga gacgcggatc gtcccggcgc gcagctgggc cttaccttcc agctccccga
     4861 tctcggacac gatcgacgtc gggtcgacgc cggcgccacc gtccgtcacc ttgagcacca
     4921 caggggtgcc cgccgcgacg gacgcggtgc gcagtctgat cagcggcggc ttcgtgtcgt
     4981 tgatccacag ccgaaagcga aacgctcccg cgcccgaggg cccagggctg tcgaagacga
     5041 tgtcgtagct cccggccttg gggcggactg cgcccgcagc cggcacgcgg tcgccgaagt
     5101 cgacgaggta cgggttgagg ttgagcggaa gcccggcgta gcctgtcagc cggttctcgt
     5161 cgccggtctc cacgacgcgc ggttccacgg ccactccacg cgcgcgctcg ataacgacga
     5221 caccgaaatt ggcaaccggc cgcttgaggg tcagccggaa gacttgctca ggacccttga
     5281 gcgagccgga gacgacacct gccggcggaa cctccggata cctgtacctg tcgacgagct
     5341 tctggcggcc acgcgtgttc ccgccgtagg tgccgggcct ggtgagggcc ttggcggggt
     5401 ggccgggcag ggcgggcacg gcaagacgca gccagtaggg gatacggcgc cgctccgcac
     5461 cgcgcgtgag gacgacgtac cctgcggcct ccccctcggc tgcggctgcc gtcgcggcga
     5521 cggtgacagt gaggtcgccg gggacggaga cgtcctttgg agcgctgacg ccgacgccgg
     5581 cccgggctac attcggcacg acggcgacgt tccagacccc ggctcccccg cccgcgtcgg
     5641 acaacgagac tgctgcgatc gcctcggagt cccggcccac gagcccgaaa gacaacgatg
     5701 tcggggaagc gaagaggagc ggccgatccg cccgcgtgag gttcacgagc cctccgcccg
     5761 ctctggtcgg ggcagcctcc gtcttcgccg actcctggcg cgataccggg tcggcggtga
     5821 cagagagtgc gctcttcagc tgcgacacgg tccagctcgg gtgccgttcg cgcagcagcg
     5881 ccgctgcgcc cgcgacgtgc ggagcggcca tgctcgtgcc cgagagggtg ccccagccat
     5941 cgggctcgga ggagaggatg ctcacgcccg gcgccgccac gtccggcttc agacggagcg
     6001 agagcggggt cgggcccgct gacgagaatc ccgccaacac cccgcggcct ccgacgcctg
     6061 cggtggtgac ggccgagacg gtgatggcgt ccgcggcgct gccgggtgac gtcaccgatc
     6121 ccggcccgaa ctcaccgaag tcgtttccgg cagctacgac ggggacgacg ccggccgctg
     6181 ccgccgcgtc caaggcgagg gcgacgaggt cgcgcgtcgg ctcgatctcc ggttcgccga
     6241 tggacagatt gatgacgtcc atgccgtcgg caacggcagc ttcgatcgcg gcgacgagct
     6301 cgggcgagtt cccgtccagg ccgacgttgg cgtcggtggg aatggtcagg gccttgtagt
     6361 tgccgagata cgcacgcgga gccacgccgg agatcgttcg tccgccgttt ccatcggttc
     6421 gcgtatcggc attgcccgcg gcgatcccgg cgacgtgcgt gccgtgcgcc gagagctctg
     6481 cgtcgaacgg tctggccgcg ttcttccagc cggcgttcgg cggcgggaag gctcgggcga
     6541 cgatcacctt tgccgtcgca taggcgcgct gccccttcgg gtatccggcg ggcagcttga
     6601 agccgctcgg gtcgaagaac ggatgctcct ggtcgatccc gtcgtcgatg atcccgatct
     6661 tgatccccgc tcctgcgctg ccaaccgcgg cgttccagag cttctgcgcc tggatttgta
     6721 gaacgctgcg atcgagctgc gggctgtagc gcactccgtg gaatatctcc tcgacacccg
     6781 gcagccgcgc aacggtcgaa acggacgccg gcgggacgac gacggccatg ccgttggcga
     6841 cgacgcgata tcgccagcgc acttcggcgc cagggacggc ctgggcgagc gcccgttcga
     6901 aacgggcgtg ctcgcgcgtg agtcgtgctt cggctgccgg gtcgcccgcc gagcgtgcga
     6961 gcgggggaga ggcgaagctc ac
//
LOCUS       DBNGNK010000018        13096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1220427,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000018 DBNGNK010000000
VERSION     DBNGNK010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 13096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13096
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1220427"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..303
                     /locus_tag="AC150V_00465"
     CDS             7..303
                     /locus_tag="AC150V_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3761427.1"
                     /translation="MVALYNDSKVQVAKTRGEFVWHSHPDTDDFFLVLSGRLTIQLRD
                     RDVELGPGELYVVPRGVEHCPRAEGEAHVLLIEPRGTPNTGDSTARDAAPEVWA"
     gene            314..637
                     /locus_tag="AC150V_00470"
     CDS             314..637
                     /locus_tag="AC150V_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HIR3761428.1"
                     /translation="MSRYKLVWFVPPSHLDATRDAVFTAGAGWIGGYARCSWCVLGAG
                     SFFGTEGTTPTAGEAGRDEHVAVYRVETVVAEDKLAAAVSALRRAHPYEEPSVDVYPL
                     ADLPA"
     gene            634..1035
                     /locus_tag="AC150V_00475"
     CDS             634..1035
                     /locus_tag="AC150V_00475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HIR3761429.1"
                     /translation="MRAKLFTDGGARGNPGPAAAAFVLEADDGTVLDARGEAIGVATN
                     NVAEYRALVAGLERAVEAGVSELEVVSDSELVVKQMQGEYRVKNKGLQPLFREASRLA
                     REIGAVTFTAVRREHNELADRLVNDALDAAG"
     gene            complement(1032..1430)
                     /locus_tag="AC150V_00480"
     CDS             complement(1032..1430)
                     /locus_tag="AC150V_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3761430.1"
                     /translation="MSPEEALGRWHAFATSGDVGLLDGLLADDVEFRPPTYWTPRRGR
                     AEVTIILGTVATVFEEFRYEREWIDGTNWALEFTARVGGLELKGVDLIRLDGDSRIAN
                     FEVVARPPNAVAALRDAMKEQLRPFLADES"
     gene            complement(1427..2041)
                     /locus_tag="AC150V_00485"
     CDS             complement(1427..2041)
                     /locus_tag="AC150V_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761431.1"
                     /translation="MQRGLYNRVLAIAGLCLLFGGFAFAVLAGTGSDSDGQAQATAQT
                     TASEQPQPKPPPPPAPPKPVAVSVKGVGAFDPEGDGRENDGEAGLATDGDATTFWASE
                     RYTSFFKNGVGLLLDAGKPVRLDRVVVRTDTPGIAASVRVGANPEGPFVTVSTTKRLA
                     ASTTFQLRPRNARYLLVWIEDIPDGSAAHVNEVRAWRAGTTATS"
     gene            2194..2832
                     /locus_tag="AC150V_00490"
     CDS             2194..2832
                     /locus_tag="AC150V_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761432.1"
                     /translation="MKFSPRKIVALGIASAALVVGGGAAIAASGDGGNDEVKAAAQHL
                     AAKTEHKSDLADKLGVTVEQLEAAHLAAATARIDAAEAAGDITAEEATSLRQALEDGR
                     MVKRLALPADVAEELGVTEEQLETAMTEVRKEEAKARVDQAVADGRITQEYADELKQQ
                     IDEADFSERGGFGKGGPGRGHHGPGGFGPGGLGGFGPGSAPPAADSGSGDEA"
     gene            complement(2916..4430)
                     /locus_tag="AC150V_00495"
     CDS             complement(2916..4430)
                     /locus_tag="AC150V_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761433.1"
                     /translation="MRRAHHVAALLGVVMVTAAALVVGLEARPASAADINPYVGPLRS
                     AVAKAEASAKQIAALEGGGKSTAAACRSAAGRLAAAAADAEKALRSYENARLRYGTQE
                     EFGLLRSLLLGLVSADAGVSRLTGTSPRPPTAGEQSAARREVGLFRQVVAARVAERFQ
                     IEGLQDVLTARSLREMRDAVINEVHGQVKTAVESELRRITGVGISLGVPLKVQIRRHA
                     ELALSRLLSKLVVNAGPAGILIQLVAGKLVDPARIVRVLGAKLREALRPKGNLAGRTT
                     QSLGTLEKNRRALNALRASSPTSRARQALRRAERALAATRFLEADLRRAGDTKRLNEI
                     ATAKKNLERTMRRTSFRFLLDAELLDYSFRDAIAFARRAQRNALAASKKCAETTPPQP
                     PPAQSNFTGTYSTTYGKMVLRQSGNRVVGCYEKDEGTLTGTVSGKMLFGTWKEEPHKT
                     DTDRGGIAFTKTGSGFSGKWNYDRNGGEAQLRKTPTSGGNWTGTISQTAPPCTP"
     gene            4525..6714
                     /locus_tag="AC150V_00500"
     CDS             4525..6714
                     /locus_tag="AC150V_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761434.1"
                     /translation="MRLRPIGAGAGIVGLALVLGWLCSLATTRVVNWFVMTDELFYER
                     LAISIAQTGSLLPRIQGEVVDNVNQLYPILLSSVFGDANVPASLVSAHRLNAFVMASA
                     AVPVYLLARRAGVGQLVSFWVGGLAAVVPWIVLSSFLLTEVVAYPVFCWAVLAITWAT
                     QRRRAVADLVALAAVGLAVAARAQFVVLALVFAVVVAGDALLGAVEDGGRGTKAVRVA
                     ATALVRTRKLLLAVYFAGFVVVIGAVAMGDGDRLLGTYSVTTSRIRVDLDLVQLALEH
                     VAILALGMAIVPFVLGVGWLVDRLRPSAPDPERALALVGVSTLLFLTLQVASFNQRFG
                     EGLVKERYLFYAVPLVLVGLAAAVASSRWPAWWAFLAPAAACVVGFASVSLPTYEKLN
                     ADSPLAVWNDEILRLAGSLDWARILLVAVTILGAALLVAAAFALPTRTVAVGIAVVAT
                     AMLPAGAVYAFDRLFAVNGTNGLPITLDQGSVFGWVDREIGTSGSVTMFRYPSNDSDF
                     WAGVGYWWNVEFYNEAIVADSRVGEAQPDPDPWAREFDPASGAMLRPGTTKYVLFHED
                     DVRLRLAGPQVEFGDHAYLYEAEQPWRAAWLTTGIYGDGFTRPHTPATIRVFSDPRQS
                     GPVKRFLTLSIRSPNDDAERPVRIGSNLGSIDAAVPPDGAIEHTAEVCVPPDGFATVV
                     VETPIVSDVYRDPSKAPLTGEIDRPAGIRLNSVVLADETAAVASCPA"
     gene            6750..7322
                     /locus_tag="AC150V_00505"
     CDS             6750..7322
                     /locus_tag="AC150V_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR3761435.1"
                     /translation="MGFFWAAVLAAAELNPGYAHRRDYLSVLAARGVEHPWLGIAAIT
                     TAGIAIVASAALLRAVSRAAALLVALAGVGFVVAAFARINCSNGAAGCGIGGRFDVSG
                     APAITHWTFTTVSTILLVAGMVVVGVMLLRRGRTLAGGGSVGAAAVTAGAFLAAGGDS
                     PGAVQRLGVLVATGWLAAVAVAELRRPRAR"
     gene            complement(7556..8029)
                     /locus_tag="AC150V_00510"
     CDS             complement(7556..8029)
                     /locus_tag="AC150V_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761436.1"
                     /translation="MRKSGVLGRRFWVTLAAGAIFALTLVVPAHAIERSTDSFQLDDV
                     PCGGGVTYDLQSTGTFLIGNTWQKVRWEGWVESNGGERLRLSHSFVEFWDKPKPAHTT
                     VVGRGFGLYGDGESIIDRGVVVFGPNGPVFMAGQHPLIEIYGGDPHAATCALLTN"
     gene            8148..>13096
                     /locus_tag="AC150V_00515"
     CDS             8148..>13096
                     /locus_tag="AC150V_00515"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nSTAND1 domain-containing NTPase"
                     /protein_id="HIR3761437.1"
                     /translation="MEFRLLGALEASEHGEQVQLGGPRQRAVLAHLLVRANEVVSTDF
                     LIDAVWSGEPPETARSTLHTYVSRLRQVVGDGASIEGRRPGYVLRVDPERIDSAQFEA
                     LVHDGRRLLARDSSAALELLEKALSLWRGTPLGDLPDDLGLRVESDRLEELRLGALED
                     RLEAELALGRHDGVVTELEALTREHPLRERLQGLLMLALYRSGRQAEALDSYQSARRL
                     LAEELGLEPSPSLRELQGRILRHDPSLESDANRLRGYRLLDAIGAGSFGVVYRAVQPH
                     VGRNVAVKVIQPALANDPRFVRRFEAEAQLVARLEHPHIVPLYDYWRDPRGAYLVMRL
                     LRGGNLEQALAAGPLGADRALALVDQVASGLAVAHRAGIVHRDVKPANVLLDDDGNAY
                     LSDFGIASDVAAELTTPTSASLEYLSPEQARGEPSTARTDVYSLGVVMRKALTGSEPP
                     GWVDEVIARATAEEQHQRYADADAFACALRESASLAVAVRRAPVRNPYKGLRAFEEAD
                     ADDFYGRDALVERLVERLAEGERFVALVGASGSGKSSVVRAGLFPALRRGAVDGSAEW
                     LLVDMAPGSAPLDELADALLAVSTTTSPRLDEARDSELLLFVDHFEELYTQVADPSRR
                     LLFLDELAAAAGKAASRVRVVVTLRADFYDRPLAHPALAELLRSGVETVVPLTPVETE
                     RSVTGPAQDVGAECESSLVAELVAEVADRPAALPLLQYALTELFERRRGGVLTLSAYR
                     ELGGLGGAVAGRADMLFEQASVDGREAIRQLFLRLVSLHAGADHVRRRVTRAELAGLE
                     VDQQELERAIEDFARHRLLSFDRDPSSRAPTIEIAHETLLESWDRLRGWIADARDDVR
                     MRERLVAAAQEWDRSEREASFLLAGSRLEHFAAWASTPRLALARNEREYLEASLARRD
                     ASRAEEDARASRERELEQRSRRRLGALVVVLAAAAATAIGLSVFAFDQRENAQAEAQR
                     AEREARLATARELAAAAQAGLVDDPERSILLALEAVEVTRAADGTVLREAEEALHEAV
                     QSSRIALSLPATLNVERGSAVAFSPDGTRIATTRDRGATIWSAETGEELFVIPSKTGR
                     LAAVAFSPDGSQIATAGREVDLWTSDTGEPIKSFEGREAGFQSIAFSPDGSRLAAGAR
                     GSTVVWEVVSGRRVATLDVAGATGLGFSTEGERLATANGSAVDVWHIATGRLALRLDR
                     MLAFDVAFRPDSSVLAVTGQGETSVVDRESGEEILVLQGHRGIVTGVDFSHDGRAVAT
                     AGHDGTVRIWDAASGRESLVLGGQGAAVQSVAFDPSGARVAACCAGGRALVWDVSARG
                     SRELVTIAAHRGSAFNVTFSPDGRRLATAGEDGAAKVFEADSGAEIVTLAAEGASPVA
                     DAVFSPDGQSLATGSFDAKARIWNLESGELLRTLAGHVAPIWGVSFAADGKSLATASE
                     DGTARIWNVETGREIHALRGHSEPVYDVAHDPSGRLAATVGNDFTIRIWDTGTGRELR
                     ALRGHEGNVVELAFSPDGNRLASAGFDAARVWDVETGDQVGVLAGHSAAVLDVDFSPD
                     GQKLATASLDGTVKLWAADTQSELLSLSTGGPGAGRLAFDPEGGRLAVGDIGGTVRLY
                     LLDPDDLVALARERVTRELTGDECRQYLHRDECAAGANR"
BASE COUNT         1833 a         4549 c         4621 g         2093 t
ORIGIN      
        1 ccgcgcatcg tggcgctcta caacgacagc aaggttcagg tcgcgaagac gcgcggcgag
       61 ttcgtgtggc actcgcaccc ggacacggac gacttcttcc tcgtgctctc ggggaggctg
      121 acgatccagc tgcgcgatcg cgacgtcgag ctaggcccgg gcgagctgta cgtcgtcccg
      181 cgcggggtcg agcactgccc gcgcgccgag ggcgaggcgc acgtgctgct gatcgagccg
      241 cgtggcaccc cgaacacggg ggactccact gcccgggacg cggcgcccga ggtctgggcc
      301 tgacggcggc ggcgtgagca ggtacaagct cgtctggttc gtgccgccct cccacctcga
      361 cgccacccgc gacgccgtct tcacggcggg ggccggctgg atcggcggct acgcgcgctg
      421 ctcatggtgc gtcctcggcg cggggagctt cttcggcaca gagggaacca ccccgaccgc
      481 gggggaagcg gggcgggacg agcacgtcgc cgtataccgc gtcgagacgg tcgtggcgga
      541 ggacaagctc gccgccgcgg tctcggcgct gcggcgcgcg cacccgtatg aggagccgtc
      601 tgtcgacgtc tacccgctcg ccgacctgcc cgcgtgaggg cgaagctctt caccgacggc
      661 ggcgcgcgcg gcaaccccgg ccccgcggcg gccgcgttcg tgctcgaggc cgacgacgga
      721 accgtgctcg acgcgcgcgg tgaggcgatc ggagtggcga ccaacaacgt tgcggagtac
      781 cgggcgctgg tggccggcct ggaacgcgcg gtcgaagcgg gcgtgagcga gctcgaggtc
      841 gtctccgact cagagctggt ggtgaagcag atgcagggcg agtatcgggt caagaacaag
      901 ggcctacaac ccctgttccg cgaggcgtca cgcctggcgc gcgagatcgg tgccgtgacc
      961 ttcaccgcgg tgcggcgtga gcacaacgag ctcgccgata ggctcgtcaa tgacgctctc
     1021 gacgcggctg gctagctttc gtccgcgagg aacgggcgga gctgttcctt cattgcgtcg
     1081 cgcaacgcgg cgacggcgtt tggcggtcgc gccaccacct cgaagttcgc gatgcgactg
     1141 tcaccgtcga ggcggatcag gtcgacgccc ttcagctcga gccctcccac gcgcgccgtg
     1201 aactcgagcg cccagttcgt gccgtcgatc cactcgcgct cgtagcggaa ctcctcgaac
     1261 acggtcgcga ctgtgccgag aatgatcgtg acctcggccc tgccccgccg cggcgtccag
     1321 taggtcggcg gccggaactc cacgtcgtcg gcgagcaggc cgtcgagcag tccgacgtcg
     1381 ccgctcgtcg cgaacgcgtg ccagcgcccg agcgcctctt cgggactcac gatgtggccg
     1441 tcgtgcctgc ccgccaggcg cgcacctcgt tgacgtgggc tgcactcccg tccgggatgt
     1501 cctcgatcca cacgagcaga tagcgagcgt ttcgtggccg tagctggaac gtcgtgctcg
     1561 ccgcgagccg cttcgtcgtc gatacggtca cgaacggccc ctccgggttg gcgccgacgc
     1621 ggacgctggc agcgatgccg ggcgtgtcgg ttcgcacgac gacgcggtcc agtctcacgg
     1681 gctttcccgc gtcgagcagc aggccgacgc cgttcttgaa gaaagacgtg tagcgctcgg
     1741 aggcccagaa cgtcgtggca tcgccgtccg tggcgagtcc ggcttccccg tcgttctcgc
     1801 gaccgtcgcc ctcggggtcg aaggcgccga ctcctttcac gctcacggca acgggtttcg
     1861 ggggcgcagg gggcggcggc ggcttgggct gcggctgctc tgacgcagtc gtctgcgccg
     1921 tcgcctgtgc ctgtccgtcg ctgtccgatc ccgttccggc gagcacggcg aaggcgaagc
     1981 ctccgaacag cagacagaga ccggcaatcg caagcacacg gttgtaaagc cctcgctgca
     2041 ctccttcgga tcgtacggaa acggcgcgct cgccgtttcc tgcgagatcg gccggccaaa
     2101 cgtctcaggg aagtcttatc tgcgcttcag gtgctcctca ggccggggcg gttccatggg
     2161 gtcgtcccca cgaacgaccg aaaggagcaa gtcatgaagt tcagcccccg caagatcgtc
     2221 gccctcggaa tcgcctccgc cgcgctcgtc gtgggcggcg gcgccgcgat cgccgcctcg
     2281 ggcgacggcg gcaacgacga ggtcaaggcc gcggcgcagc acctggcggc caagacagag
     2341 cacaagagcg acctcgccga caagctcggt gtcacggtcg agcagctcga ggctgcccac
     2401 ctggccgcgg caacggcccg gatcgacgcc gcagaggccg ccggagacat caccgccgag
     2461 gaggcgacct cgctccggca ggcgctggag gacggccgca tggtgaagcg cttggcgctt
     2521 cccgccgatg tggcagagga gctcggcgtc accgaggaac agctcgagac ggccatgacc
     2581 gaggtccgca aggaagaggc caaggcgcgc gtcgaccagg cggtcgccga cggccgaatc
     2641 acgcaggagt acgccgacga gctgaagcag cagatcgacg aggccgactt cagcgagcgt
     2701 ggcggtttcg gcaagggcgg ccccggccgc ggccaccacg gacccggcgg cttcgggccg
     2761 ggcggtctcg gcggcttcgg ccccggctcc gcgccaccgg ctgccgactc cggctcggga
     2821 gacgaggcgt agagccgatt cccgtcagcc gttgtttcga gggcccgctc cggcgggccc
     2881 tcatccgttc gggccgtgcc ggacggctgc cggcgtcacg gcgtgcaggg cggagctgtc
     2941 tgactgatcg tgcccgtcca gttcccgccg ctcgtgggcg tcttcctgag ctgcgcctcg
     3001 ccgccgttgc ggtcgtagtt ccacttgccg ctgaagccgc ttcctgtctt cgtgaatgcg
     3061 atgccgccac gatcggtgtc ggtcttgtgc ggctcctcct tccaggtccc gaagagcatc
     3121 ttcccggaca cggtgccggt gagggtcccc tcgtccttct cgtagcagcc gacgacgcgg
     3181 ttcccgctct gcctcagcac catctttccg taggtggtgc tgtaggtgcc ggtgaaattg
     3241 ctctgcgccg gcggcggctg gggcggtgtc gtctcggcgc acttcttcga cgccgccagc
     3301 gcgttgcgct gcgcgcggcg cgcgaaggcg atcgcatcgc ggaagctgta gtcgaggagt
     3361 tccgcgtcga ggaggaagcg gaagctcgtg cggcgcatcg tacgttcgag gttctttttc
     3421 gcggtcgcga tctcgttcag ccgcttggta tcgccagcgc gccgcagatc ggcctcgagg
     3481 aagcgggtcg ccgcgagggc ccgctccgct cgacggagcg cttggcgcgc ccgagacgtc
     3541 ggtgagctcg cgcggagggc gttcagagcg cggcggttct tctcgagtgt ccccagcgac
     3601 tgcgtcgtgc ggccggcgag gttgccttta ggccgcagcg cctcccggag cttggcgccg
     3661 agcacgcgga cgatccgtgc gggatcgacg agcttcccgg ccacgagctg gatgaggatt
     3721 cctgcgggcc ccgcgttgac gacgagcttg gagagcaggc gggagagggc cagctcggcg
     3781 tgccggcgga tctgaacctt gagcgggacg ccgagcgaga tgccgacccc ggtgatgcgg
     3841 cggagctcgc tctcgaccgc ggtcttgacc tggccgtgaa cctcgttgat cacggcgtcg
     3901 cgcatctccc gcaggctgcg ggcggtcagg acgtcctgca ggccctcgat ctggaatcgc
     3961 tcggcgactc gtgcggcgac gacctgccgg aagaggccga cctcgcggcg ggcggcgctc
     4021 tgctcccccg cagtcggcgg acgaggcgag gtgccggtca gcctgctcac gccggcgtct
     4081 gcgctcacca gcccgagcag cagtgagcgc agcaggccga actcctcctg cgtcccgtag
     4141 cgcaggcgcg cgttctcgta gcttcgaagc gccttttcgg cgtcggctgc agcggccgca
     4201 agccggcctg cagccgagcg gcacgcagcg gctgtgctct tgccgcctcc ttcgagcgca
     4261 gcgatctgct tcgcactggc ttcggccttc gcgacggcac tccgaagcgg cccgacgtac
     4321 gggttgatgt cggctgcaga agcaggtcgg gcctcgagcc cgacgacgag agctgctgct
     4381 gtgaccatca cgaccccgag cagcgctgcg acgtggtgcg cccgtcgcat acgccgtgag
     4441 tgtttcaggc cttcgcgggc aggtcaaacg gtcgtctgcg agcgcgctac gctggcacgc
     4501 cgctgctata ggtagcggac gggcatgcga ctgcgaccga tcggggccgg agctgggatc
     4561 gtcgggctcg cgctcgtcct ggggtggctg tgctccctgg cgacgacgag ggtcgtcaac
     4621 tggttcgtga tgacggacga gctgttctac gagcggctcg cgatctcgat cgcccagacc
     4681 ggctcgctgc tccctcgcat ccagggcgag gtggtcgaca acgtgaatca gctgtatcca
     4741 atcctcctgt cgtccgtgtt cggcgacgcg aacgttccgg cgagcctggt ctccgcgcac
     4801 cggctcaacg cgttcgtgat ggcctccgcc gcagtccccg tctacctgct cgcgcgcaga
     4861 gctggagtcg gccagctcgt gagcttctgg gtcggcggcc tagccgcggt tgtgccctgg
     4921 atcgtgctct cgtcctttct cctcaccgag gtcgtcgcct accccgtgtt ctgctgggcg
     4981 gtgctcgcga tcacgtgggc gacgcagcgg cgccgggctg tcgccgacct ggtggcgctc
     5041 gctgccgtcg gcctcgccgt cgcggcgcgc gcccaattcg tcgtgctcgc gctcgtcttc
     5101 gccgtggtgg tcgcgggcga cgcgctgctc ggcgctgtcg aggacggcgg gcggggaacg
     5161 aaggccgtcc gcgtcgcggc gacggcgctc gtccggacgc gcaagctgct cctcgccgtc
     5221 tacttcgcgg gtttcgtcgt cgtgatcggc gcggtcgcca tgggcgacgg cgaccggctc
     5281 ctcgggacgt actcggtgac gacgtcacgc atccgcgtcg acctcgacct cgttcagctc
     5341 gccctcgagc acgtggcgat tctcgcgctc ggcatggcga tcgttccctt cgtgctcggt
     5401 gtcggctggc tcgtcgatcg cctgcgcccg tcggcgccgg accccgagcg cgcgctggcg
     5461 ctcgtggggg tctcgacgct gctcttcctc acactccagg tggcgtcgtt caaccagcgc
     5521 ttcggcgagg ggttggtgaa agagcggtac ctcttctatg ccgtcccgct cgtgctcgtc
     5581 ggcctcgcgg ctgcggtcgc ttcgagtcgt tggcccgcct ggtgggcgtt cctcgccccc
     5641 gcagccgcct gcgttgtcgg gtttgcgtcg gtctcgctgc cgacgtacga gaagctcaac
     5701 gcggactcgc cgctcgccgt ctggaacgac gagattctcc gcctcgcggg ctcgctcgac
     5761 tgggcgcgaa ttctcctggt cgccgtgacg atcctgggcg ccgccctgct ggtcgctgcc
     5821 gccttcgcgc tcccgacgag gacggtcgcg gtcgggatcg ccgtggtcgc gacggcgatg
     5881 ctcccggccg gcgcggtcta cgccttcgat cggctgttcg ccgtgaacgg gacgaacgga
     5941 ctcccgatca cgctcgacca gggcagcgtg ttcggatggg tcgaccgcga gatcggcacc
     6001 agcgggagcg tgacgatgtt ccgctacccg tcgaacgact cggatttctg ggctggagtc
     6061 ggctactggt ggaacgtcga gttctacaac gaggcgatcg tcgccgacag ccgagttgga
     6121 gaagcacagc ccgaccccga cccttgggcg cgggagttcg acccggcctc gggagcgatg
     6181 ctgcgacccg gaaccacgaa gtacgtcctc ttccacgagg acgacgtccg cctccgactg
     6241 gccgggccgc aagtcgagtt cggcgaccac gcctacctgt acgaggccga gcagccctgg
     6301 cgggcggcct ggctcaccac cggcatctac ggtgacggct tcacccggcc gcatacgccg
     6361 gcgacgatca gggtcttctc cgatccgcgg cagagcggcc cggtgaagcg gtttctgacg
     6421 ctttcgatca gatcaccaaa cgacgacgcg gagcggccgg tgcgcatcgg ctccaacctc
     6481 ggcagcattg acgccgccgt tccgcccgac ggcgcgatcg aacacaccgc ggaggtgtgc
     6541 gttccgcccg acggcttcgc gaccgtcgtc gtcgagacgc cgatcgtgtc ggatgtctac
     6601 cgcgatccga gcaaggcgcc gctgaccggt gagatcgacc ggcccgcggg gatacgactc
     6661 aactctgtgg tgcttgccga cgagacggcg gcggtggcga gctgcccagc ctgagccaca
     6721 ggggatcggc cgcgcttgcc ctcgcgggca tcggcttctt ctgggcggcc gtgctcgccg
     6781 cggccgagct gaaccccggc tatgcgcatc gccgcgacta cttgagcgtg ctcgccgccc
     6841 gtggggtgga gcatccctgg ctcgggattg ccgcgatcac cacggccgga atcgcgatcg
     6901 tcgcgtcggc cgccctgctg cgggcggtct cccgcgccgc ggcactcctc gtggcgctcg
     6961 cgggagtggg cttcgtcgtc gccgctttcg cgcgcatcaa ctgctcgaac ggcgccgccg
     7021 gctgtggaat cggcggccgg ttcgacgtct ccggcgcacc ggcgatcacg cactggacgt
     7081 tcacgacggt ctcgaccatc ttgctcgtgg ccggcatggt cgtagtcggc gtcatgctgc
     7141 tgcgacgcgg ccgaaccctg gccgggggcg gctcggtcgg cgcggcggcg gtgacggcgg
     7201 gcgcgttcct cgccgctggc ggggactccc cgggcgccgt acagcggctg ggcgtcctcg
     7261 ttgccaccgg ctggctcgcc gccgtggccg tcgccgagct ccggcgtccg cgggcccggt
     7321 agcagctgtc agccctgcac gaccgggcgg tcgatgaccg agccctgcct gtcgagctcg
     7381 gcccctcgcg cgcgctgccc ctcgcgcgcg ctgccccagg tggccgtgcg tgaagtgcac
     7441 cagtagcgcc accgcgaccc agagggtcac cgccgagagc gcgagctcga acgctgtcgt
     7501 cgcatgctcg agcggctccg cggccgacgg ccgggagccg ctcgagtgag cacgatcagt
     7561 tcgtcaggag cgcgcaggtc gccgcgtggg gatcgcctcc atatatctcg atcagcgggt
     7621 gctggcccgc catgaagaca gggccgttcg gtccgaacac cacgacacct cggtcgatga
     7681 tcgactcccc gtcgccgtat agcccgaatc ctcgaccgac cactgtcgtg tgagcaggct
     7741 tcggcttgtc ccagaactcg acgaacgagt ggctcagccg aaggcgctca ccgccgttgg
     7801 actcgaccca accctcccag cgcaccttct gccaggtgtt tccgatcaag aaggttcccg
     7861 ttgactgcag gtcgtacgtg actccgcctc cgcacgggac gtcgtcgagc tggaagctgt
     7921 cggtgctccg ctcgatcgca tgtgcgggca cgaccagcgt cagggcaaag atcgcgccgg
     7981 ccgcgagcgt gacccagaag cgccggccaa gaacgccaga tttcctcatt ttcgtctcct
     8041 ttttgctcgg cgtgtgcgac acgaacgagc ttcgccgagg ccccttgcaa gctccttgca
     8101 gaccgccggg aaaccacccc ttttcgcgac gtcgtacgat tcgacacgtg gagttccggt
     8161 tgctcggcgc tcttgaggcc tccgagcacg gagagcaggt gcaactcggg ggtccgcggc
     8221 agcgtgccgt tctcgcgcat ctcttggtgc gcgcgaacga ggtcgtctcg acggacttcc
     8281 tgatcgacgc cgtctggagc ggcgagccgc ccgagaccgc ccgcagcacc cttcacacct
     8341 acgtgtccag gttgcgccag gtggtcggcg acggcgcgag tatcgaagga cggcgcccag
     8401 gctacgttct ccgggtcgac cccgagcgaa tcgactctgc gcagttcgag gctctcgtcc
     8461 acgatggtcg tcgcctcctc gcgagggaca gcagcgctgc tctcgagctg ctcgagaagg
     8521 cgttgagcct gtggcggggt acgccgctcg gagacctccc cgacgacctc ggtcttcgcg
     8581 tcgagagtga ccgcctcgaa gagctgcgcc tgggagccct cgaggaccgt ctcgaggccg
     8641 agctggcact cggccgccac gacggggtcg taaccgagct cgaagcgctg acgagggaac
     8701 acccgttgcg cgagaggctc cagggtcttt tgatgctcgc tctctatcgc tcgggtcgac
     8761 aggcagaggc gctcgactcc tatcagagcg ctcgacgcct gctcgccgag gagctcggtc
     8821 tcgagccgtc gcccagtctg cgggagctcc aaggccgcat cctccggcac gacccgtctc
     8881 tcgagtccga tgcgaaccgc ctacgcggat atcgactgct ggacgcgatc ggcgccggga
     8941 gcttcggcgt cgtctaccgg gccgtccaac cgcacgtcgg ccgaaacgtc gcggtgaagg
     9001 tcatccagcc ggcgctcgcc aacgatccgc gcttcgtgcg caggttcgag gccgaggcgc
     9061 aactcgtcgc ccggctcgag cacccccaca tcgtgccgct ctacgactac tggcgtgatc
     9121 cgcgcggtgc ctatctcgtg atgcggctgc tgcgcggcgg gaatcttgag caggcgctcg
     9181 cagcgggccc gctgggggcc gaccgggcct tggcgctggt cgaccaggtc gcctccggac
     9241 tcgccgtcgc ccatcgagcc ggcatcgtcc atcgtgacgt caagccggcg aacgtcctgc
     9301 tcgacgacga cggcaacgcg tacctgtccg actttgggat cgccagcgat gttgccgccg
     9361 agctcacgac gccgacctca gccagtctcg agtacctgtc gcccgagcag gcgcgcggcg
     9421 agccgtccac cgcccgcacc gacgtgtaca gcctcggcgt ggtcatgcgc aaggccctca
     9481 ccggctctga gccccccggg tgggtcgacg aggtgatcgc acgcgctacg gcggaggagc
     9541 aacatcaacg gtatgcggac gcggacgcgt tcgcatgtgc actccgagaa tcggcctccc
     9601 ttgcggtcgc tgtacgacgg gctcctgtcc gcaatcccta caagggcctc cgggccttcg
     9661 aggaggcaga cgccgacgac ttctacggcc gcgacgcgct cgtcgagcgg ctcgtcgagc
     9721 gattggccga gggcgagcgc ttcgtggccc tcgtaggcgc gtccgggagc ggcaagtcct
     9781 ccgtcgtccg ggccggcctc ttcccggccc tgcgtcgagg cgcagtagac ggatcggcgg
     9841 aatggctgct cgtcgacatg gcccccggct ccgctcctct cgacgagctg gcggatgcgc
     9901 tgctggcggt ctcgacgacg acctctcccc gcctcgacga agcgcgcgac tccgagctac
     9961 tgctcttcgt cgaccatttc gaggagctgt acacgcaggt ggcggacccg tctcgccggc
    10021 tgcttttcct cgacgagctc gccgcagccg ctggcaaggc ggcaagcaga gtgcgcgtgg
    10081 tcgtcactct gcgggccgac ttctacgacc ggccactagc gcacccggcg ctggccgagc
    10141 tcctgcgctc gggggtcgag acggtcgtcc ccctgacgcc ggtagaaacg gagcggtctg
    10201 tgaccgggcc cgctcaggac gtcggggcgg agtgcgaatc gagcctcgtc gcggagctcg
    10261 tcgcggaggt cgccgatcgg ccggccgcgc tgcccctgct ccagtacgcc ctaacagagc
    10321 tcttcgagcg gagacgcggc ggcgtgctga cgctgtccgc gtatcgcgag ctcggcgggc
    10381 tgggtggagc ggtcgccgga cgcgccgaca tgctcttcga gcaggcgagc gtcgacggcc
    10441 gggaggcgat ccggcagctg ttcctgcgcc tcgtgagctt gcacgccgga gcagatcatg
    10501 tgcgccggcg agtcacgcgg gcggagctcg ccggtctcga agtcgatcag caggagctgg
    10561 agcgggcaat cgaagacttc gcgcgacatc gactgctttc tttcgatcga gatccgagct
    10621 cgcgggcgcc gacgatcgag atcgcgcacg agacattgct cgagagctgg gaccggttgc
    10681 gcggctggat cgcggacgcg cgcgacgacg tacggatgcg cgagcgactc gtcgccgcgg
    10741 cgcaggagtg ggatcggtcc gaacgagagg cgagcttcct cctcgcgggt agccggctcg
    10801 agcacttcgc tgcgtgggcc tcgacgcccc ggctcgcgct cgcgcgcaac gagcgcgagt
    10861 acctcgaggc gagcttggcg aggcgcgacg cgtcgcgtgc ggaggaggac gcccgcgcgt
    10921 cgcgcgagag ggagctggag cagcgctcgc gcaggaggct cggtgcactc gtggtcgtgc
    10981 tcgccgccgc cgccgccacc gcgatcggcc tgagtgtgtt cgccttcgat caacgcgaga
    11041 atgcgcaggc cgaggcgcag agagccgagc gcgaagcgcg attggcaact gcacgcgagc
    11101 ttgcggcggc ggcgcaggcc ggtctcgtcg acgatcccga gcggagcatt ctgctcgcgc
    11161 tcgaggccgt cgaggtcacc cgcgcggccg acggcacggt cctcagggag gccgaggagg
    11221 cgctgcacga ggccgtacag agctcgcgca tcgccctctc gctgcccgcc acgctgaacg
    11281 tggagcgtgg cagcgcggtc gccttcagcc ccgacggcac caggatcgcg acgacgcgcg
    11341 accgtggggc cacgatctgg tcggcggaga cgggcgagga gctcttcgtc attccgagca
    11401 agacgggacg gctggccgcc gttgccttca gcccggacgg ctcgcagatc gcgacggcgg
    11461 gtcgagaggt tgacctgtgg acttccgaca cgggtgagcc aatcaagtcc ttcgagggcc
    11521 gagaggcggg tttccagagc atcgccttta gccctgacgg ctcacgcctc gctgcgggag
    11581 cgcgtgggtc gacggtggtc tgggaggtcg tctcgggccg gagggtcgcg acgctcgacg
    11641 tcgccggcgc cacagggctc ggattcagta cggagggcga gcgattggcc accgcgaacg
    11701 gatcagccgt ggacgtctgg cacatcgcca cgggtcggct cgctctgcga ctcgaccgga
    11761 tgctcgcgtt cgacgtcgcc ttccgtcccg acagctcggt gctcgcggtg acggggcagg
    11821 gtgagacgag cgtcgtcgat cgcgagagcg gcgaagagat cctggtgctg caaggtcacc
    11881 gaggcatcgt cacgggcgtc gacttcagtc acgacggccg cgcggtagcg accgccgggc
    11941 atgacggcac cgtgcgtatc tgggacgcgg cttcgggtcg agagagtctt gtcctcggcg
    12001 gacagggcgc ggcagtccaa agcgtcgcct tcgaccccag cggcgcgcgt gtggcggcat
    12061 gctgcgccgg cggccgcgct ctcgtctggg acgtaagcgc acggggcagc cgtgagctcg
    12121 tgacgatcgc cgcccaccgc gggtcggcgt tcaatgtcac gttcagcccc gacggccggc
    12181 gcctcgccac ggccggcgag gacggtgcgg ccaaagtctt cgaggccgac tctggcgcag
    12241 agatcgtcac gctcgccgcg gaaggcgcca gtcccgtcgc cgacgcggtc ttcagccccg
    12301 acggccagag cctcgcgacg ggcagcttcg acgcgaaggc gcgcatctgg aacctcgaaa
    12361 gcggcgagct gcttcggact ctggccggcc acgtcgcgcc catctggggc gtatcgttcg
    12421 ccgccgacgg gaaatccctc gcgacggcga gcgaggacgg gaccgcccgg atctggaacg
    12481 tcgagacggg cagggagatt cacgcactcc gcggccactc ggagcccgtc tacgacgtcg
    12541 cgcacgatcc ctctggacgg ctcgccgcga cggtcgggaa cgacttcacc attcgcatct
    12601 gggacacagg cacgggtcgt gagctccgtg ctctgagggg ccacgaaggc aatgtcgtgg
    12661 agctcgcgtt cagccccgat ggaaatcgcc tggcgagcgc cggcttcgac gcggcgcggg
    12721 tctgggacgt ggagaccgga gaccaggtcg gcgtcctcgc gggtcacagc gcagccgttc
    12781 tcgacgtcga cttcagtccg gacggccaga agctcgccac cgccagcctc gacgggacgg
    12841 tcaagctctg ggcggccgac acgcagagcg agctcctcag cctgtcgaca ggtggccccg
    12901 gagccggccg cctcgccttt gatcctgagg gtggccggct tgcggtcggc gacatcggcg
    12961 gcaccgtccg cctgtacctc ctcgatcccg acgacctcgt cgcgcttgcc cgagagcggg
    13021 tcacccgcga gctgaccggg gacgagtgtc gccagtatct gcaccgggac gagtgcgccg
    13081 caggcgccaa caggta
//
LOCUS       DBNGNK010000019         8598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1005786,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000019 DBNGNK010000000
VERSION     DBNGNK010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8598
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1005786"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="AC150V_00520"
     CDS             complement(<1..496)
                     /locus_tag="AC150V_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761438.1"
                     /translation="MSIAQVEQRVLEEEAPSGLWREAWRRLRTNKGALAGFVLIGLFV
                     LAGIFAPLVAPYDPRDQDLSLIRDGCCPGPSWKHPFGVDALGRDEFSRVIYGARFSLA
                     IGVVSVMLGLSIALVLGAVSGFVGGKIDTVIMRAMDIMLAVPGLLLAIGIVAVLGPGL
                     VQIMI"
     gene            complement(493..1497)
                     /locus_tag="AC150V_00525"
     CDS             complement(493..1497)
                     /locus_tag="AC150V_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761439.1"
                     /translation="MARYAVRRLLMLIPILIGLSILVFLFIRALPGGPAQALLGERAT
                     EESVAEIEATYGLDQPIYVQYVKYVKQIASGNLGVSITSRRSVKEEIGRRFPATFELA
                     IAAMFIAVVVGVPLGYVAAKRHGTWVDHTSLFAALIGISIPVFLLGILLKYIFAVKLG
                     LLPTVGRIDPLISADHPTNFYVLDAILTLNFAAFWSSVEHLILPSIALATIPLAIVAR
                     ITRASVLDVQNEDYVRTARAKGLPNRTVGRRHVFRNALLPVTTIVGLQVGLLLAGAVL
                     TETVFAWPGMGTWLVDAIDARNYPVLQGGILFIAIVFVFVNLIVDISYAYINPRIRLS
                     "
     gene            complement(1536..3212)
                     /locus_tag="AC150V_00530"
     CDS             complement(1536..3212)
                     /locus_tag="AC150V_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3761440.1"
                     /translation="MRKRSIAFVAAGIVALAALAAGCGGGGGEGDETLIFGAAADPVL
                     LDGALISDGESLRVVDQILEGLTNLKPGTTEIEPGLAESWETSADGLEWTFTLRDGVT
                     FHDGEPFNAEAVCFNYDRWYNFKGDLQSDSASYYWGAVFGIGGNAFADSEGPALYESC
                     EAVDELTAKITLTQPSAAFLGALALTNFGIASPKALQEYNADEGQVDAEGVFQSGGTY
                     GTEHPTGTGPFKFVEWIPGDKLVLERYDDYWGEPAKLQQLIFRPISDNAARLQALQND
                     EIQGYDLVEPQDIETIEGDADLQLLDRPPFNVGYVTINQAMPPMDNVLVRQVVAHGLD
                     RQAVVDGFYAGRGEVANEFMPPSLEGYADDVVTFDYDPEKAKALLQEAGLELPVEIEF
                     WYPTDVSRPYMPDPKRNFEAFAASLEESGFKVTPKSAPWNPTYLGRQQAGTAGHLNLI
                     GWTGDYGDPDNFIGTFFRNPDPAWGTDKKPNEEVVSILEEALSEADIDARTALYEEAN
                     RKIAEWIPGVPYVHTSPALAFRADVTGYEPSPVSLEEFAPVSYGSEEESG"
     gene            complement(3351..4421)
                     /locus_tag="AC150V_00535"
     CDS             complement(3351..4421)
                     /locus_tag="AC150V_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3761441.1"
                     /translation="MTQSQLIELLESEQMKGLIERAEERGYVEPVELEAVALEHELDE
                     RDAETLQRELEAIGLEIGAPPKAPAEKPSAEAAAQAAQALSGAADSLQLFLADVGRHK
                     LLTAAQEVALAKRIERGDPTAKRHMLESNLRLVVSIAKGYRGLGVPFLDLIQEGTLGL
                     NRAVGKFDWRRGYKFSTYATWWIRQSVQRAVANHARTIRVPVHVVERQQKLSRAARRL
                     EVELGRDATREELAEATGLPLQHVEEALGAAHASVSLNQTVGADDEGELGDLFADRDA
                     ADPFEEAEESLRRQGVRRALDALPERERRILELRFGFEGEPWTLEAIGHELDLTRERV
                     RQLEGQALSRLAALRDLASLAA"
     gene            complement(4674..7250)
                     /gene="topA"
                     /locus_tag="AC150V_00540"
     CDS             complement(4674..7250)
                     /gene="topA"
                     /locus_tag="AC150V_00540"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR3761442.1"
                     /translation="MSPKTNNPGTRLVIVESPAKARTIASYLGDGFEVASSVGHIRDL
                     PNKAAEIPPKMKKEPWARLGVDVEHDFQPIYVVDPDKKKRVAELRKKLAHANELLLAT
                     DEDREGEAIAWHLVEVLKPKVPVRRMVFHEITRDAIERALDETREIDDRLVDAQETRR
                     ILDRLYGYEVSPVLWKKVMPRLSAGRVQSVATRLVVERERERRAFVSAEYWDIVGMFD
                     PGSFEAKLSTLNGRRVAQGRDFTSKGDLKSDDSVQLDEEAARGLAQRLEGARFAVRGL
                     DEKPYTRRPAAPFMTSTLQQEASRKLRFTSQTTMSVAQRLYENGFITYMRTDSTTLSE
                     SALQAARAQAAQLYGDESVPAQPRRYERKVKNAQEAHEAIRPAGDAFRLPSEVKGELS
                     RDEQALYELIWKRTIASQMADASGRTVTLRLGAVSSADEDAEFSTSGTVITFRGFLAA
                     YEEGRDEESNGNGRRSEGEQRLPELETGQHVDVASLEPTGHSTSPPARFTEATLIRAL
                     EERGIGRPSTYASIIGTILDRGYVFKRGTALVPSFVAFSVTGLLEQHFGQLVDYDFTA
                     RMEDDLDRIAAGQEERNAWLDRFYHGTGQAPGLHQLVTDHLDEIDARAVNSIPVGEGI
                     VLRVGRYGPYLERGEQRANVPPDLAPDELTVELAEKLLAQPNGDRPLGSDPETGHEIV
                     AKSGRYGPYVTEVLPEGVKEKPRTASLLSSMSIETVTIEDALRLLSLPRSLGEHEGEE
                     VIAANGRYGPFVKKGSESRSLESEEQLFSISLTDALELLAQPKRRRGRGGAKRGPLRE
                     LGKDPGSGKPIVVKDGRFGPYVTDGDTNASLRRGDSVESLTLERGVELLAERRAKVAA
                     AG"
     gene            7351..7980
                     /locus_tag="AC150V_00545"
     CDS             7351..7980
                     /locus_tag="AC150V_00545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3761443.1"
                     /translation="MANHPRPSWFYYLVGGLSWPVLRAFYRLSPKGLENLPAESGFVL
                     AANHTSNFDPWPLGIPLYPQRYLRFMGKSELFWFPLGPVLQAGGAFPVKRGQRDSAAI
                     DTAVRLCREGHVVVMFPEGTRRSKGLRKKLEPKWHAGAARIALEAGVPLVPAAVAGTD
                     RLSRLEKLRVVYGAPIDMGDLVDVPDADAAQVATERLREAILALEQTLR"
     gene            7977..>8598
                     /locus_tag="AC150V_00550"
     CDS             7977..>8598
                     /locus_tag="AC150V_00550"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR3761444.1"
                     /translation="MRPLLAVDGDSFAHRAYHALPKSIRRGDDGPAGALTGFMSMLLR
                     LWEAERPRAVLVAWDTLSELTYRHSELPGYQSGRVFDDELLEQLDLLPDFVESTGIVC
                     ARAAGYEADDFLGAAARTEERSGGLVLVATSDRDAFQLASDRTTILQPKRGVSSLVRV
                     GPNEVRERYGVEPEQVPDFIALRGDPSDKIPGARGVGEKTAAALLQQ"
BASE COUNT         1295 a         2890 c         2928 g         1485 t
ORIGIN      
        1 cgatcatgat ctggacgaga cccgggccaa ggacggccac gatcccgatc gcgaggagca
       61 ggcccggaac ggcgagcatg atgtccatcg cccgcatgat caccgtgtcg atcttgccgc
      121 cgacgaagcc cgagaccgca ccgagtacca gtgcgatcga caacccgagc atgacggaga
      181 cgacgccgat cgcgagcgag aagcgtgcgc cgtagatgac acgcgagaac tcgtcgcggc
      241 cgagtgcgtc gaccccgaac gggtgtttcc aggacgggcc tggacagcag ccgtcgcgga
      301 tcagagacag gtcctggtcg cgggggtcgt agggcgcgac caagggcgcg aagatcccgg
      361 caaggacgaa gaggccgatc aggacgaagc cggcgagcgc gcccttgttc gtccgtagac
      421 ggcgccacgc ctcacgccag aggccgctcg gcgcctcttc ttcgagcacc cgttgctcga
      481 cctgggcgat gctcaactga gacggatcct cgggttgatg taggcgtagg agatgtcgac
      541 gatcaggttc acgaagacga agacgatcgc gatgaagagg atcccgccct gcagcaccgg
      601 gtagttgcga gcgtcgatcg cgtcgacgag ccacgtgccc atgccgggcc aggcgaagac
      661 cgtctcggtg agcacggcgc cggcgagcag caggcccact tgcaggccga cgatcgtcgt
      721 caccggcagc agagcgttcc gaaagacgtg cctccggccg accgttcggt tgggcagtcc
      781 cttcgcgcgc gccgtgcgca cgtagtcctc gttctgcacg tcgagcacgg aggcccgggt
      841 gatccgcgcg acgatcgcga gcggaatcgt ggcgagtgcg atcgacggca ggatcaggtg
      901 ctcgaccgag gaccagaagg cggcaaagtt gagggtcaag atcgcgtcga ggacgtagaa
      961 gttcgtcggg tggtcggcgc tgatcagcgg gtcgatccgg ccgacggttg gtaagagccc
     1021 gagcttcacc gcgaagatgt acttgagcag gatcccgagc aggaagacgg ggatcgagat
     1081 gccgatcagg gcggcgaaga gactcgtgtg gtccacccac gtgccgtggc gtttggcggc
     1141 gacgtagccc aaggggacgc cgacgacgac ggctatgaac atcgccgcaa tcgccagctc
     1201 gaaggtggcc gggaagcggc ggccgatctc ttccttgaca gagcggcggc tcgtgatcga
     1261 gaccccgagg ttgccgctcg cgatctgctt gacgtacttg acgtactgga cgtagatcgg
     1321 ctgatcgagc ccgtaggtcg cctcgatctc ggcgaccgat tcctccgtcg cgcgctcgcc
     1381 gagcagggcc tgtgccgggc cgccgggcag agcccggatg aagaggaaga ccaggatcga
     1441 cagcccgatc aggatcggga tgagcatgag caggcggcga acggcgtagc gagccatgtc
     1501 cctacgccgt cggccgcctg cgcttcgtca cggtgttagc cgctctcctc ctcggagccg
     1561 taggagaccg gcgcgaactc ctccagcgag accgggctcg gctcgtagcc cgtcacgtcc
     1621 gcacggaacg cgagcgcggg gctcgtgtgc acgtacggca cgcccgggat ccactcggcg
     1681 atcttccgat tcgcttcctc gtagagcgcc gtccgagcat cgatgtccgc ctcgctcagg
     1741 gcctcttcca ggatgctcac aacctcctcg ttcggcttct tgtccgtccc ccaggcgggg
     1801 tccgggttcc ggaagaacgt gccgatgaag ttgtccgggt caccgtagtc gccggtccag
     1861 ccgatcaggt tcaggtggcc cgcggtgccc gcctgctggc gcccgaggta ggtcgggttc
     1921 cagggggcgc tcttcggtgt caccttgaag ccggactcct ccaggctcgc cgcaaaggcc
     1981 tcgaagttgc gcttcgggtc cggcatgtaa ggccgggaga cgtcggtcgg gtaccaaaac
     2041 tcgatctcca ccgggagctc gagcccggcc tcttgcagca gcgccttcgc cttctccggg
     2101 tcgtagtcga acgtgacgac gtcgtccgcg tagccctcga ggctgggggg catgaactcg
     2161 ttcgccacct cgccgcggcc ggcgtagaag ccgtcgacga ccgcctggcg gtcgaggccg
     2221 tgcgcgacga cctgccggac gaggacgttg tccatcggcg gcatggcctg gttgatcgtc
     2281 acgtagccga cgttgaacgg cggccggtcg aggagctgca gatcggcgtc gccctcgatc
     2341 gtctcgatgt cctgcggctc gacgaggtcg tatccctgga tctcgtcgtt ctgcagcgcc
     2401 tgcaagcgtg cggcgttgtc ggagatcgga cggaagatga gctgctgcag cttggccggc
     2461 tcaccccagt agtcgtcgta gcgctcgagc accagcttgt cgcccggaat ccactcgacg
     2521 aacttgaacg ggcccgtccc ggtcggatgc tcggtgccgt acgtgccgcc ggactggaag
     2581 acgccttcgg cgtcgacctg gccctcgtcg gcgttgtact cctgcagcgc cttcgggctg
     2641 gcgatgccga agtttgtcaa ggcgagcgcg ccgaggaacg ccgcggacgg ctgggtcagc
     2701 gtgatcttgg cagtgagctc gtcgacggcc tcgcagctct cgtagagagc gggcccctcg
     2761 ctgtcggcga aggcgttgcc gccgatcccg aacactgcgc cccagtagta ggaggcgctg
     2821 tccgactgga ggtcgccctt gaagttgtac caacggtcgt agttgaagca gaccgcctcg
     2881 gcgttgaacg gctcgccgtc gtggaacgtg accccgtcgc ggagggtgaa cgtccattcg
     2941 aggccgtccg ccgaggtctc ccacgactcg gccaggccgg gctcgatctc cgtcgttccc
     3001 ggcttgagat tcgtgaggcc ctccaggatc tggtcgacca cgcgcagcga ctcgccgtcg
     3061 gagatcagcg ccccgtcgag cagcaccggg tcggcagccg ctccgaagat cagtgtttcg
     3121 tcgccctcgc cgcctccgcc gccgcagccg gccgcgagcg ccgcgagcgc gacgatcccg
     3181 gccgccacga acgcgatcga acgtttccgc atccctcgca cagccactcc tttcactccg
     3241 tctgcatcga tgtgtgctgc gagtatccgg gacagacagt cctgggagca acccttccgt
     3301 cgtgccgggc ccccgccttc gggaaccggg cctggcagct tgatccgctc ttacgccgcc
     3361 agtgaggcca gatcgcggag cgctgcgagg cgtgagagcg cctgcccttc gagctgccgg
     3421 acgcgttcgc gcgtaaggtc gagctcgtgc ccgatcgcct cgagcgtcca cggctctcct
     3481 tcgaacccga agcggagctc gaggatgcgc cgctcccgct cggggagagc gtccagggcg
     3541 cgccggaccc cctgcctccg gagcgactcc tcagcctcct cgaacgggtc ggcggcgtcg
     3601 cgatcggcga acagatcacc gagctcgccc tcgtcgtcgg cgccgacggt ctgattcagc
     3661 gagacggaag cgtgggcggc gccgagcgcc tcttcgacgt gctggagcgg cagcccggtg
     3721 gcttccgcga gctcctctcg cgttgcgtca cggccgagct ccacctcgag ccggcgtgct
     3781 gcgcgtgaca gcttctgctg ccgctcgacc acgtgaaccg gaacgcggat cgtgcgcgca
     3841 tgattcgcga ccgcgcgctg cacggactgg cggatccacc aggtcgcgta cgtcgagaac
     3901 ttgtagccgc ggcgccagtc gaacttcccg acggcgcggt tgaggccgag cgtgccttcc
     3961 tggatgagat cgaggaatgg gacgccgagc ccccgatacc ccttggcgat cgagaccacc
     4021 agccgcagat tcgactcgag catgtgacgc ttcgcggtgg ggtcgccccg ctcgatccgc
     4081 ttggccagag ccacctcctg ggcggcggtg agaagcttgt gccggcccac atcggcgagg
     4141 aagagctgca gcgagtccgc ggctccactc agcgcctgag cggcctgtgc ggcggcctcg
     4201 gcgcttggct tctcggcagg cgcttttggc ggcgctccga tctcgagccc gatcgcctcc
     4261 agctcgcgtt gcagcgtctc ggcatcgcgc tcgtccaact cgtgctccaa cgcgacggcc
     4321 tccagctcca cgggctcgac gtagccgcgc tcctcggcgc gctcgatcag ccccttcatt
     4381 tgctccgact cgagcagttc gatcagctgg ctctgtgtca atctcgttcg atgtccttcc
     4441 gtgtcgccgg ggcacaaacc tgacgcactc tatggggtga ttcgactggt ctccaaggtt
     4501 gagcggatat cgagggtgtt acaagctcgc aacgtgcgcc attttctcct tattccgctg
     4561 ccgaggcgat tcggcttgcc ggggccatcg tgcgagcctg tggcggctca cacatgatac
     4621 gactccggac ggacgcttga agttagaggg cgtctaggtt tttcgaggcc gtgtcaaccc
     4681 gccgcggcga ccttcgcacg gcgctccgcg agcagttcca cgcctcgttc gagcgtcagc
     4741 gattcgacgc tgtccccccg ccgcaagctc gcgttcgtgt cgccgtccgt tacgtagggg
     4801 ccgaagcgac cgtccttgac gacgatcggc ttcccgctcc ctgggtcttt cccgagctcc
     4861 cgcagcgggc cacgcttcgc tccgccccgc ccgcggcggc gcttcggctg cgcgagcagc
     4921 tcgagcgcgt cggtgagcga gatgctgaac agctgctcct cgctctcgag agatcggctc
     4981 tcggatccct tcttgacgaa cggcccgtac cgtccgttcg cggcgattac ctcctcgccc
     5041 tcgtgctcgc cgaggctccg tgggagcgag aggaggcgaa gagcgtcctc gatcgtcacg
     5101 gtttcgatgg acatcgagct gagcagcgaa gcggtccgcg gcttttcctt cacgccctcg
     5161 ggcagcacct cggtgacgta ggggccgtag cgcccggact tcgccacgat ctcgtggccg
     5221 gtctcgggat cggatccgag cggacgatcg ccgttcggct gcgccaggag cttctcggcg
     5281 agctcgaccg tgagctcgtc gggagcgaga tcgggcggga cgttcgcccg ctgctcgccc
     5341 cgctccagat atgggccgta gcggccgact cgaagcacga ttccttcccc gaccggtatc
     5401 gagttgacgg cccgtgcgtc gatctcgtcg aggtggtcgg tgacgagctg gtggaggccg
     5461 ggcgcctgtc cggtgccatg gtagaagcgg tcgagccacg cattgcgttc ctcctggccg
     5521 gccgcgatcc ggtcgaggtc gtcctccatc cgggcggtga agtcgtagtc gacgagctgg
     5581 ccgaagtgct gctcgagcag gccggtgacg gagaaggcca cgaaagacgg caccagcgcc
     5641 gtcccgcgct tgaacacgta gccgcgatcg agaatcgtgc cgatgatcga ggcgtacgtc
     5701 gacggtcgcc cgatgccgcg ttcctccagg gcgcgaatca gtgtcgcctc cgtgaaccgt
     5761 gcgggtgggg aggtcgagtg gccggtcggc tcgagtgacg cgacgtcgac gtgctggcct
     5821 gtttcgagct ccggaaggcg ctgctcgccc tcggagcgcc ggccattgcc gttcgactcc
     5881 tcgtcccgtc cttcctcata cgccgcgagg aagccgcgga aggtgatgac cgtgcccgag
     5941 gtgctgaact cggcgtcctc gtcggccgag gaaacggcgc ccaggcggag ggttacggtg
     6001 cggccgctgg cgtctgccat ttgggaggcg atcgttcgct tccagatcag ctcgtacaag
     6061 gcctgctcgt cgcgcgagag ctcgcctttg acctcggacg ggagccggaa cgcgtcgccc
     6121 gccgggcgga tcgcctcgtg cgcttcctgt gcgttcttca ccttccgctc gtagcgccgc
     6181 ggctgtgcgg ggaccgactc gtccccgtag agctgagccg cctgcgcccg cgccgcctgc
     6241 agcgcggact cggacagcgt cgtcgagtca gtgcgcatgt atgtgatgaa gccgttctcg
     6301 tacagacgct gggccacgct catcgtcgtc tgggaggtga agcgaagctt gcggctcgcc
     6361 tcctgctgca aggtcgacgt catgaagggc gcagccgggc ggcgggtgta cggcttctcg
     6421 tcgagcccgc ggaccgcgaa gcgggcacct tcgagccgct gcgccagccc ccgggctgct
     6481 tcctcgtcca gctgcaccga gtcgtcgctc ttgaggtctc ccttcgaggt gaagtcgcgc
     6541 ccctgggcca cccgtcgccc gttgagcgtg ctgagcttgg cctcgaacga gcccgggtcg
     6601 aacatcccga cgatgtccca gtactcggcc gagacgaacg cacggcgctc gcgctcgcgc
     6661 tcgacgacga ggcgcgtcgc aacggactgg acgcgtccgg ccgagagccg cggcatcact
     6721 ttcttccaga gcacggggga gacctcgtag ccgtagaggc ggtcgaggat ccgtctcgtc
     6781 tcctgggcgt cgacgagccg gtcgtcgatc tcgcgcgtct cgtccagagc ccgttcgatc
     6841 gcgtcgcggg tgatctcgtg gaagaccatg cgccgtacag gaaccttcgg cttcagcacc
     6901 tcgacgagat gccacgcgat cgcctcgcct tcgcggtctt cgtcggtcgc gagaaggagc
     6961 tcgttcgcgt gggcgagctt cttgcgcaac tcggccacac gcttcttctt gtccggatcg
     7021 accacgtaga tcggctggaa gtcgtgctct acgtccacac cgaggcgcgc ccagggctcc
     7081 ttcttcatct tgggcgggat ctcggcagcc ttgttcggca aatcgcggat gtgcccgacg
     7141 ctcgatgcca cctcgaagcc gtcgccgagg tagctcgcga tcgtccgcgc cttggcgggc
     7201 gattcgacga tcacgagtcg tgtgccgggg ttgttcgtct tgggactcat gcgaacctgt
     7261 tcggccgatg tgtcggctcg attcgcgcga caccttagcg tcgcgggtcg gcgacgccgg
     7321 taggttcgag gcgcgatact ccgccgcaga gtggccaacc acccgcgtcc gagctggttc
     7381 tactacctcg tcggggggct cagctggccc gtgctgcggg cgttctatcg gttgagcccg
     7441 aagggtctcg agaacctgcc tgcggagagt ggttttgtgc tcgcggccaa ccacacctcg
     7501 aacttcgacc cgtggccgtt gggcataccg ctgtatccgc agagatacct ccggttcatg
     7561 gggaagtccg agctgttttg gtttccgctg ggccccgtgc tccaggccgg cggcgcgttt
     7621 ccggtcaagc gcgggcagcg ggacagcgca gcgatcgaca ctgccgtgcg cctctgccgc
     7681 gagggtcatg tcgtcgtgat gttccccgaa ggaacgcggc ggtcgaaggg tctacgcaag
     7741 aagctcgagc ccaagtggca cgccggcgcc gcgcggatcg cacttgaggc aggcgtcccg
     7801 ctcgtgccgg ccgctgtggc gggaaccgat cgcctgagcc ggctcgagaa gctccgggtc
     7861 gtctacggcg cgccgatcga catgggcgat ctcgtcgacg tgccggacgc agatgccgcg
     7921 caggtcgcga ccgagcgctt gcgcgaggcg atcctcgcgt tggagcagac cctgcggtga
     7981 ggccgctgct cgcagtcgac ggcgactcct tcgcccaccg cgcgtaccac gccctgccga
     8041 agtcgatccg caggggcgac gacggcccgg ccggtgctct cacgggcttc atgtccatgc
     8101 tgctcaggct ctgggaggca gagcggccac gcgcggtgct ggtcgcctgg gacaccctgt
     8161 cggagctcac ctaccgtcat tccgagctcc ccggctatca gtccggccgt gtcttcgacg
     8221 acgagctcct cgagcaactc gatctcctcc ccgacttcgt cgagtcgacc ggaatcgtct
     8281 gtgcgagggc ggcggggtac gaggccgacg acttcctcgg agccgcggcc cgcacggaag
     8341 agcgctccgg tggactcgtt ctggtggcca cgtcagatcg cgacgcgttc cagctcgcca
     8401 gcgaccggac gacgatcctg cagccgaagc gcggcgtgag ctcgctcgtg cgggtcggtc
     8461 cgaacgaggt cagagagcgg tacggggtcg agccggagca ggtacccgac ttcatcgcgc
     8521 ttcgcggtga tccctcggac aagattccgg gcgcgcgcgg cgtgggggag aagaccgcgg
     8581 ccgcgctcct gcagcaat
//
LOCUS       DBNGNK010000020         3310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1651012,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000020 DBNGNK010000000
VERSION     DBNGNK010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1651012"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="AC150V_00555"
     CDS             <1..649
                     /locus_tag="AC150V_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3761445.1"
                     /translation="EVISRLVDGSRFAEFKALYGDTLVCGFARIEGYPVAILANRGVL
                     FAESSQKGAHFVELACKRRIPLVFLQNITGFMVGKEYEEGGIARDGAKLVMAVACADV
                     PKFTVVIGGSFGAGNYAMCGRAYEPRQLWMWPNARISVMGGDQAATVLTMVGDADPDE
                     IKATYEEEGSPYYSTARLWDDGIIDPLDTRRVLALGLAAAANGPVPETTFGVFRM"
     gene            complement(657..1556)
                     /locus_tag="AC150V_00560"
     CDS             complement(657..1556)
                     /locus_tag="AC150V_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3761446.1"
                     /translation="MILAVATNGAVRGLVDRYGMRLGAARFVAGETLEECIPVLRRLN
                     ARGLHANTTILGENVTVPAAAIAAADEYERVLERIAGESLRVNVALKLTHLGLELDEE
                     LAFANVARVVDAASRFGNFVRIDMEQSSTVDATLRVYRRLRETGRDNVGAVLQSCLRR
                     TDVDLEGVLPLRPNIRIVKGAYLEPAEVAYTDKAAVDASFARHVATLLRAGCHAAVAT
                     HDERLIEWTISLAEREGVGRDRFEFQLLYGVRPKLQLDLAGRGFKVLVATPYGPEWYP
                     YLMRRLAERPANVAFLAGNVLRR"
     gene            complement(1571..2419)
                     /locus_tag="AC150V_00565"
     CDS             complement(1571..2419)
                     /locus_tag="AC150V_00565"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3761447.1"
                     /translation="MLTGALAAAVTPLRQGGLALDEDAFAPYATFLAVGGLTGVLALG
                     TTGEGILLSVPERKRAVELFVEACAGSLDVAAHCGAQTTVDTVELAEHAAASGADAVA
                     VIGPPYFKLDEDEQLAHLAAAAHACAPTPFYVYEFAATAGYPIAVDVLLRLRDRAPNF
                     EGMKVSDRPWEAFEPYLLEGLGVLVGPEFLIHRGMEAGAVGAVSALAAAFPEEVAAVV
                     AKPTADGAERLADLRAGVERFPRHAALKHVLVSRGVPLGEDVRAPLRQLTREERRELD
                     AWLALP"
     gene            2444..3199
                     /locus_tag="AC150V_00570"
     CDS             2444..3199
                     /locus_tag="AC150V_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR3761448.1"
                     /translation="MGALRIEREDDVLRVTLARPETRNAFDAALIAELARAFVDVGKA
                     RAVVLAGDGKSFCAGADVEWMRASADLTYDENVADAKALRQMLAAIDACPAPVVAAVQ
                     GHALGGGAGLVACADIVLTHEQTVFAFSEVKLGIIPAVISPFAVRKIGESAARRYFVT
                     GERFDAETALRIGLVHEVTGDLDAALAVLLDELRTAGPRAARHAKKLVLDGPDGPETA
                     RRIAERRTSEEGQEGLSAFLERRPPAWAPIDPD"
BASE COUNT          520 a         1153 c         1169 g          468 t
ORIGIN      
        1 ggaggtcatc agccgcctcg tcgacggcag ccgcttcgcc gagttcaagg cgctctacgg
       61 cgacaccctc gtctgcggct tcgcgcggat cgaggggtac cccgtcgcga ttctcgcgaa
      121 ccggggcgtg ctcttcgccg agtcgtcaca gaagggcgca cacttcgtcg agttggcctg
      181 caagcgtcgg ataccactcg tcttcctgca gaacatcacg ggcttcatgg tcggcaagga
      241 gtacgaggaa ggcggcatcg cccgtgacgg agcgaagctg gtgatggcgg tggcctgcgc
      301 ggacgttccc aagttcaccg tcgtgatcgg cggctcgttc ggagcaggca attacgccat
      361 gtgcggacgc gcatacgagc cgagacagct ctggatgtgg ccaaacgccc gcatctcggt
      421 gatgggaggc gaccaggcgg cgaccgtgct cacgatggtg ggcgacgccg atcccgacga
      481 gatcaaggcg acgtacgagg aggagggcag cccctactac tcgacggcgc gactctggga
      541 cgacggcatc atcgacccgc tggatacccg acgcgtgctt gcgctcggac tcgcggccgc
      601 agcgaacggc cccgtgccgg agaccacctt cggcgtcttc cgcatgtagc tcgggctcag
      661 cgccggagca cgttgccggc gaggaaggcg acgttggccg gtcgctcggc caggcgccgc
      721 atgaggtacg ggtaccactc cggcccgtac ggcgtcgcga cgagcacctt gaagccacgg
      781 ccggccaggt ccagctgcag ctttggacgc actccgtaga gcaactggaa ctcaaaccgg
      841 tcacgtccta ctccctcgcg ctccgccagc gagatcgtcc actcgatcaa tcgctcgtcg
      901 tgggtcgcga cggccgcgtg gcaacccgcc cgcagcagcg tcgcgacatg gcgggcgaag
      961 ctcgcatcga ccgctgcctt gtcggtgtat gcgacctcgg cgggctccag atatgcgccc
     1021 ttgacgatgc ggatgttggg ccgtagcggg agcacgcctt cgaggtcgac gtcggtccgg
     1081 cgcaggcagg actggagcac cgccccgacg ttgtcgcgcc cggtctcgcg aaggcggcgg
     1141 tagacgcgga gcgtcgcgtc gacagtgctc gactgctcca tgtcgatcct gacgaagttg
     1201 ccgaatcgac ttgccgcgtc gaccacgcgc gcgacgttgg cgaaggcgag ctcctcatcg
     1261 agctcgagcc cgagatgggt gagcttcagc gcgacgttga cccgcagcga ctcgcccgcg
     1321 atccgttcca gcacccgctc gtactcgtcg gccgcggcga tcgcggccgc ggggacggtc
     1381 acgttctccc cgagaatcgt cgtgttggcg tggagcccac gtgcgttcag ccgtcgcagg
     1441 acgggaatgc actcctcgag cgtctcgccc gcgacgaagc gggcggcacc gagccgcatg
     1501 ccgtaccgat cgaccagccc gcgtaccgcg ccgttcgtcg cgaccgcgag gatcaccgac
     1561 cgcgccgcgc tcacggcaac gcgagccagg cgtcgagctc gcgcctctcc tcccgtgtga
     1621 gctgcctgag cggagcgcgc acgtcttcgc cgagcggcac gccgcgcgac acgaggacgt
     1681 gcttcagcgc agcgtgccgc gggaagcgct cgacgcccgc ccgcagatcg gccagccgct
     1741 cggcgccgtc ggccgtcggc tttgcgacga ccgccgccac ctcctcggga aaggctgccg
     1801 ccagtgcgga gaccgcgccg accgcgccgg cctccatgcc gcggtggatg aggaactcgg
     1861 ggccgacgag gacgccgagg ccctcgagga ggtacggctc gaacgcttcc cacgggcgat
     1921 cggagacctt catgccctcg aagttgggcg cccggtcgcg caggcggagg aggacgtcca
     1981 ccgcgatcgg gtagccggcg gtggcggcga actcgtacac gtaaaaggga gtgggagcgc
     2041 acgcatgcgc agcggcggcc aagtgggcga gctgctcgtc ctcgtcgagc ttgaagtacg
     2101 gcggcccgat gaccgcgacc gcgtccgcgc cggacgccgc agcgtgctcg gcgagctcga
     2161 ccgtgtcgac ggtcgtctgg gcgccgcaat gtgctgcgac gtcgagcgag ccggcgcacg
     2221 cctccacgaa gagctcgacg gcacgcttgc gctcgggtac ggagagcagg attccctcac
     2281 ccgtcgttcc gagcgcgagc acgcctgtca ggccgccgac cgcgaggaag gtcgcatacg
     2341 gtgcgaaggc atcctcgtcg agcgctaatc ccccctgtcg cagaggcgtg accgccgcgg
     2401 cgagagcacc ggtcagcatg gcgcggactc tatgctgccg cgcgtgggag cgttgcggat
     2461 cgagcgggag gacgacgtgc tacgggtcac gctggcgcga cccgagacgc gcaacgcctt
     2521 cgacgctgcg ctgatcgccg aattggccag ggcgttcgtc gacgtcggca aggcacgggc
     2581 cgtcgtgctc gccggggacg ggaagagctt ctgcgcagga gccgacgtcg agtggatgcg
     2641 agcgtccgcg gacctcacgt acgacgagaa cgtcgccgac gcgaaggcgc tgcgacagat
     2701 gctcgcggcg atcgacgcct gtcccgcacc ggtggtggcg gccgtccagg ggcatgcgct
     2761 cggcggcggt gccggcctcg tggcctgtgc ggacatcgtg ctcacccacg agcagaccgt
     2821 cttcgccttc tcggaagtga agctcgggat catcccggcg gtgatctcac cgttcgcggt
     2881 gcgcaagatc ggcgagagcg cggcgcgacg ctacttcgtc accggcgagc gcttcgacgc
     2941 cgagacggcg ctgcgcatcg gcctcgtgca cgaggtgacc ggcgacctcg atgccgcact
     3001 cgcagtactg ctcgacgagc tccgcaccgc aggcccgcga gcggcccgcc acgcgaagaa
     3061 actcgtactc gacggccctg acggccccga gaccgcgcgc cgtatcgccg agcgacgcac
     3121 gagcgaggag ggccaggagg ggctcagcgc gtttctcgag cggcgcccgc ccgcttgggc
     3181 tccgatcgac ccggactgac caccggccgc gggaccgcca ccggctgcgc atgcgcgaac
     3241 cgcccgccgg tcggcctaga gttgccgcgt gcagatcacg cgcctgctcg tggcgaaccg
     3301 cggcgagatc
//
LOCUS       DBNGNK010000021         1481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_3432,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000021 DBNGNK010000000
VERSION     DBNGNK010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_3432"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1071
                     /locus_tag="AC150V_00575"
     CDS             172..1071
                     /locus_tag="AC150V_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR3761449.1"
                     /translation="MRVGITGGSGLVGSALAASLSAAGDGVLRFVRREADGPDEVSWD
                     PSGGIVDAVRLEGLDAVVHLSGKRIDSRWSRAAKEEIRASRVDTTQLLAETLAQLERP
                     PPVLLTASAVGYYGSRGDETLSEASGAGEGFLAELCEAWEAASAPAIEAGIRVVNARF
                     GVILTTEAPPLASALRPFKLGLGAVLGDGRQYMSWVHLDDAIAAIHYLLSAESLSGPV
                     NVTAPNPVTNRQFTKALAATLGRPAWFRLPRFAVGTMFGEMGRQTILASQRAVPTKLE
                     HSGFAFAYPELPAALAREIGGGD"
     gene            1073..>1481
                     /locus_tag="AC150V_00580"
     CDS             1073..>1481
                     /locus_tag="AC150V_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3761450.1"
                     /translation="MPNATQQLRIGEFSRRVGVPAELLRAWERRYGLFEPARTEGGFR
                     LYSEADVQRALAMQANVGRGLSASEAASLALEEEAAEAAKGAFADVVQQLDDRLEHFD
                     EVGAQSVLDRLLSTVSLEAVLSEVLIPYFRLLGE"
BASE COUNT          213 a          491 c          530 g          247 t
ORIGIN      
        1 cacgtaggtc tctttccagt cgagccgatg aagtctccgg ctagctctca tgctgttctt
       61 ggatattctt gtatagtata tgtccagatt acgagctctt agaggagccc gatgtcgaga
      121 gaacgtcaat cagagcacac ctccggtacg gccgccgcgg agccgaagac gatgcgcgtg
      181 ggaatcacgg gcgggtcggg cctcgtcggc tccgcgctcg ccgcctcgct gtctgcggct
      241 ggggacgggg tgctccggtt cgtccgcaga gaggccgacg ggcccgacga ggtgagctgg
      301 gacccatctg gcgggatcgt cgacgcggtg cgcctcgagg gtctggacgc ggtcgtgcac
      361 ctgtccggga agcggatcga ctcccggtgg tcgcgggccg cgaaggagga gatccgggcg
      421 agtcgagtcg atacgacgca gctgctggcg gagaccctcg ctcagctcga gcggccgccg
      481 cctgtactcc tgaccgcgtc ggccgtcggc tactacggca gccgtggcga cgagacgctt
      541 tcggaggcga gcggagccgg cgagggcttc ctggctgagc tctgcgaagc ctgggaggcg
      601 gcctccgccc ccgcgatcga ggcggggatc cgcgttgtca acgcccgctt cggcgtgatc
      661 ctgacgaccg aagcgccacc gcttgcctcc gcgctccgtc ccttcaagct cgggctcggc
      721 gccgtcctcg gcgacggtcg ccagtacatg agttgggtgc acctcgatga cgcgatcgca
      781 gcgattcact atctgctctc ggcggagtcg ctctcggggc ccgtcaacgt gactgctccg
      841 aacccggtta ccaaccggca gttcaccaag gcgctcgctg cgacgctcgg gcggcccgcc
      901 tggttccggc tcccccggtt cgcggtcggg acgatgttcg gcgagatggg gcgccagacg
      961 atcctggcaa gccagcgtgc cgtgccgacg aaactcgagc actcgggctt cgcgttcgcg
     1021 tacccggagc tgccggccgc gctggctcgc gagatcggcg gcggagactg acgtgccgaa
     1081 cgcgacgcag cagctacgga tcggcgagtt ctcccggcgg gtgggcgtcc cggccgagct
     1141 cctgcgggcc tgggagcgac ggtacggcct cttcgagccc gcacggacgg aaggtgggtt
     1201 ccggctgtac tcggaggcgg acgtccagcg ggcgcttgcg atgcaggcga acgtcggtcg
     1261 cgggctgtcg gcctcggagg cggcgagcct cgccctcgag gaagaagcgg ccgaagcggc
     1321 aaagggcgct ttcgcggacg tcgtgcagca actcgacgac cggctcgagc acttcgacga
     1381 ggtcggcgcg cagtccgtgc tcgaccgact tctctccacc gtctcgctcg aggcggtgct
     1441 cagcgaagtc ttgatcccgt acttccgcct gctcggcgag c
//
LOCUS       DBNGNK010000022         4451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_934797,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000022 DBNGNK010000000
VERSION     DBNGNK010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_934797"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="AC150V_00585"
     CDS             complement(<1..248)
                     /locus_tag="AC150V_00585"
                     /inference="COORDINATES: protein motif:HMM:NF016005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3761451.1"
                     /translation="MDRPLRLITSNPVDALARTLESLLVVASAPLSIDELADAAGDDA
                     ERIETALGLLGDRYREGRSGIVLEEVAGGWAFRASRESA"
     gene            complement(227..982)
                     /locus_tag="AC150V_00590"
     CDS             complement(227..982)
                     /locus_tag="AC150V_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR3761452.1"
                     /translation="MTAVRDLELDLDAFEGPFDLLLTLLLKEALEPKDVDIAAIVVAF
                     VDHLATRDELDLDACGEFLVLIAALLELKARLLFPDEEAELAELEPEEAAEELARRLA
                     EYRRMKEAAIWLSERLATERDRYFRLGPAPLAPEPEHTLPAQDVALLASALRGLAVEP
                     PNVSLAHMALRFPPVSQFLERFRAVLSRRRRFDFDAEVGGLPRVEQAVAFLALLELRK
                     SGEIALAQASPFAPIRVSRADHERILEWTAHSA"
     gene            complement(995..1867)
                     /gene="lgt"
                     /locus_tag="AC150V_00595"
     CDS             complement(995..1867)
                     /gene="lgt"
                     /locus_tag="AC150V_00595"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR3761453.1"
                     /translation="MSSLLALGSIPSPSSSTVDVGPFTLHMYGLTLLVAIVAATYITG
                     RRWVARGGEWDLILRCAVWGVAAGIIGARLYHVVTSWDELPDEWWGPFAIWKGGLGVW
                     GGIGFGVVAGGIVAWRAGADVWLLGDCLAPALLVAQAIGRVGNWWNQELFGKPSDLPW
                     ALEIDPANRPLEYLDAATFHPTFLYEALWALGAAGLLLLIERRWRIHPPGLFALYVTI
                     YCIGRLWVELLRIDPAHEIAGLRLNAWVSMLGIAVGVVWFVVSQRHGRRRERGDVPER
                     ETMVVPKGRVRSER"
     gene            complement(1917..2126)
                     /locus_tag="AC150V_00600"
     CDS             complement(1917..2126)
                     /locus_tag="AC150V_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761454.1"
                     /translation="MSMSAADIQTTVDSEMEIVEQWRHEALRRAGYDPEAATVLAASH
                     DIDLHEAVSLLERGCSVELALQILL"
     gene            2370..2786
                     /locus_tag="AC150V_00605"
     CDS             2370..2786
                     /locus_tag="AC150V_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR3761455.1"
                     /translation="MRLRKNAKLDLLATVPLFTECSTRELSEIAQIADELDLPSGTVL
                     IREGERGREFFVLVEGSLSVSKNGRRVTSLDAGTWVGEMALLSDAPRNATVTATSPIH
                     VLVITDRAFQRLVKSSPSIALNVLASVSERLKPDNA"
     gene            2880..3320
                     /locus_tag="AC150V_00610"
     CDS             2880..3320
                     /locus_tag="AC150V_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005553172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3761456.1"
                     /translation="MARLVGINHVALDVGSLDEALEFWERLFGPLTLRGRSGGMAFVD
                     MGDQFVALAAGPTLPPDAMRHVGIVVDDRQAVLDAARAAGAEVRGNDVLDPWHNVLQI
                     VDYRDVQFTKAPEVLRAMGAEDLRKSERALAELAAKGIEPPQRD"
     gene            complement(3328..>4451)
                     /locus_tag="AC150V_00615"
     CDS             complement(3328..>4451)
                     /locus_tag="AC150V_00615"
                     /inference="COORDINATES: protein motif:HMM:NF025930.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3761457.1"
                     /translation="ALISSVMNTKDRVPFYVNACDEMGIEVLPPDVNSSQTDFAVVEG
                     KIRFGLNAVKNVGDSAARLIVAARDEGGGFGSIWELTDRVDPQFVNKRALESLVKCGA
                     LDSTGASRLGMLEVLEQALAYGQKQAADRLAGQASIFDLGMGSEQDGGDEPGQPHYPA
                     VPPGEFDKAELLRLEKETLGLYVSEHPLSSVREQLRRKTDATLSELERRRDGEVVTVG
                     GIVSEVRPMTTKKGDPMAYMTLDDPTGTADVVVFNSTYKDARELCVEDTVIVVKGRID
                     HRRQGETMLVAMEVAGFEAVPERRDVRFRIDATKARAGIIRELAELVRDFPGESPVYV
                     SVETSVGPKMLALGPEYRVRPDSDFLAEAKALLGEAAVV"
BASE COUNT          725 a         1568 c         1486 g          672 t
ORIGIN      
        1 gcagactcgc gcgaggcgcg gaacgcccag cctcccgcga cctcctccag gacgatgccg
       61 ctgcgccctt cccgataccg gtcgcccagg agcccgagcg cggtctcgat ccgctcggcg
      121 tcgtcgccgg ccgcgtccgc gagctcgtcg atcgagagcg gtgccgacgc gacgaccagc
      181 aacgactcga gggtgcgcgc cagcgcgtcg acgggattgg acgtgatcag gcggagtggg
      241 cggtccattc gaggatcctt tcgtggtcgg cgcgggaaac cctgatcggc gcgaacggtg
      301 acgcctgcgc aagcgcgatc tcgcccgact tgcgcagctc gagcagcgca aggaaggcca
      361 cggcctgctc gacacgcggc agtcccccga cctcggcgtc gaaatcgaag cgccgacgcc
      421 gcgacagaac ggcgcgaaaa cgctcgagga actgcgagac gggaggaaag cgaagcgcca
      481 tatgggcaag agagacgttc ggcggctcga ccgccaaccc tcgcagcgcc gaggcgagga
      541 gtgccacgtc ctgcgccggg agcgtgtgct ccggctcggg ggcgagtggc gcgggcccga
      601 gccggaaata ccggtcgcgc tccgtcgcga ggcgctccga cagccagatc gcggcttcct
      661 tcatccggcg gtattcggcg agtcgccgcg cgagctcctc cgccgcctcc tccggctcga
      721 gctcggccag ctcggcctcc tcgtccggga agaggaggcg ggccttcagc tcgagcaagg
      781 cggcgatcag gacgaggaac tctccgcagg cgtccaggtc tagctcgtcc cgtgtcgcca
      841 ggtgatcgac aaacgcgacc acgatcgccg cgatgtcgac gtccttcggc tccagcgcct
      901 ctttcagcag cagcgtgagc aggaggtcga acggcccctc gaaggcatcg aggtccagct
      961 cgagatcgcg gacggcggtc atcctgtcgc gaccctagcg ctcggaccgg acgcgcccct
     1021 tcgggacgac catcgtctcc ctctccggca catcgccgcg ctcgcggcgg cgcccgtggc
     1081 gctgcgagac cacgaaccag acgacgccga cggcgatgcc gagcatcgac acccacgcgt
     1141 tgagccggag gccggcgatc tcgtgcgcag ggtcgatgcg caacagctcg acccacagcc
     1201 ggccgatgca gtagatcgtc acgtagagcg cgaacaggcc gggcggatgg atccgccagc
     1261 ggcgctcgat cagcaggagc agaccagccg cgccgagcgc ccataacgcc tcgtagagga
     1321 acgtcggatg gaaggtcgcc gcatccaggt actcgagcgg tcggttcgcg ggatcgatct
     1381 cgagcgccca gggaaggtcg ctcggcttgc cgaagagctc ctggttccac cagttgccga
     1441 cgcgaccgat cgcctgagcg acgagcagag ccggggcgag gcagtcgccg agaagccaga
     1501 cgtccgcacc cgcgcgccac gcaacgatgc cgccggcgac caccccgaac ccgatgccgc
     1561 cccagacgcc caaaccacct ttccagatcg cgaagggccc ccaccactcg tcgggcagct
     1621 cgtcccagct cgtcacgacg tggtacaggc gggcaccgat gatcccggcc gcgacgcccc
     1681 agaccgcaca gcggaggatc aagtcccatt cgccgccgcg cgcgacccag cgtcggcccg
     1741 tgatgtaggt cgccgcaacg atcgcgacga gcagcgtcag cccgtacatg tggagcgtga
     1801 agggccccac gtcgacggta ctcgagcttg gggaaggaat cgaaccgagc gcgagcagcg
     1861 agctcatccg actgattctg ccggtcggcg cggggtgcgc gccgatttgg cgcagcctag
     1921 agcagtattt ggagggcgag ctcgaccgag caaccgcgct cgagcaggct cacagcctcg
     1981 tggaggtcga tatcgtgcga agcagcgagg acggtggcgg cctcggggtc gtagccggcg
     2041 cgccgcagcg cctcgtgtcg ccactgctcg acgatctcca tctcggagtc gaccgtcgtc
     2101 tgaatatccg ccgcagacat gctcactctc tcactctctc ctgacgcttt cggttacccg
     2161 gatattcgtc ggcaacggtg gatcggatgc cccctttcta ggtgattccg ataaaggttt
     2221 cgtaaaagcg ctatgaacgt ttcgtgggcc acgcgcgccg agcgtgcctc gcccgcgtcg
     2281 cggcgcaccc gctcggggac gctcgaaacg cgcccggaat gcggcgagag gcgcttcact
     2341 tttcggatct gcggcagtac gatcgccgcg tgcggctgcg gaagaacgcg aagcttgacc
     2401 tgctcgcgac ggttcccctg ttcacggagt gctcgacgcg cgagctgagc gagatcgccc
     2461 agattgccga cgagcttgac cttccgtcgg gcaccgtcct gattcgcgag ggggagcgcg
     2521 ggcgcgagtt cttcgtcctc gtggagggat cgctctcagt ttccaagaac ggccgccggg
     2581 tcacctcgct cgacgcgggg acgtgggtcg gagagatggc gcttctctcc gacgcgcccc
     2641 gcaacgccac ggtgaccgcg acgtcgccga tccacgtgct cgtcatcacc gaccgggcgt
     2701 tccagcgtct ggtcaagagc tcgccgtcga tcgcgctgaa tgttctcgcg agcgtctccg
     2761 agcgcctcaa gcccgacaac gcgtaggcag ctcttccttg gctcttcatc ccgcccgtcg
     2821 gcggcgcgtg cgggtgtccg ccttcagaga ggacggccgg ctcggctaga gtcggcggcg
     2881 tggcgcgcct ggtcggaatc aaccacgtcg cgctcgacgt gggatcgctc gacgaggcgc
     2941 tcgagttctg ggagcgcctg ttcggcccgc tgacgttacg aggtcgctcc ggcggtatgg
     3001 cgttcgtcga catgggggat cagttcgtcg ctcttgccgc cggcccgacc ctgccgccgg
     3061 acgcgatgcg ccacgtgggc atcgtcgtcg acgaccggca ggcggtcctc gacgccgccc
     3121 gtgcggccgg cgccgaagtg cgcggcaacg acgtgctcga cccgtggcac aacgtgctcc
     3181 agatcgtcga ctaccgcgac gtacagttca cgaaggcccc cgaggtgctc cgagcgatgg
     3241 gggccgaaga cctcaggaag tcggagcggg cactcgccga gctggccgcg aagggaatcg
     3301 agccacccca gagggactag ttccggctca gacgaccgcg gcttcgccga gcagcgcttt
     3361 cgcctcggcg aggaagtccg agtcgggacg gacgcggtac tcggggccga gcgcgagcat
     3421 cttgggcccc accgaggtct cgaccgagac gtagacgggc gactcgccgg ggaagtctcg
     3481 aacgagctcg gccagctcac ggatgatccc ggcgcgcgcc ttcgtcgcgt cgatcctgaa
     3541 ccgcacgtcg cgccgctccg gcaccgcctc gaagccggcg acctccatcg cgacgagcat
     3601 ggtctccccc tgacgccggt ggtcgatccg gcctttcacg acgatcacgg tgtcctcgac
     3661 gcacagctcc cgcgcatcct tgtaggtcga gttgaagacg acgacgtccg cagtccccgt
     3721 cgggtcgtcg agcgtcatgt acgccatcgg atcgcccttc ttcgtcgtca tcggccgcac
     3781 ctccgagacg atcccgccga ccgtgaccac ctcgccgtcg cgccggcgct cgagctcgga
     3841 gagcgtggcg tcggtcttcc gcctgagctg ttcgcgcacc gacgagagcg ggtgctcgga
     3901 gacgtacaga ccgagcgtct ccttctcgag gcggagcagc tcggctttgt cgaactcgcc
     3961 cgggggaact gcggggtagt gcggctgccc cggctcgtcg cctccatcct gctcgctgcc
     4021 catgccgaga tcgaagatcg acgcctgccc ggccagccgg tctgctgcct gcttctgacc
     4081 gtatgcgagc gcctgctcga gcacctcgag catccccagc ctggacgcac ccgtcgagtc
     4141 gagcgccccg cacttcacca acgattccag tgcccgcttg ttgacgaact gcggatcaac
     4201 gcgatcggtg agctcccaga tcgagccgaa cccgccgccc tcgtcacgcg cggcgacgat
     4261 caggcgggcg gcgctgtcgc cgacgttctt caccgcgttc aggccgaaac ggatcttgcc
     4321 ctcgacgacg gcgaagtcgg tctgggacga gttgacgtcg ggcggcagca cctcgatccc
     4381 catctcgtcg cacgcgttga cgtagaacgg cacgcgatcc ttcgtgttca tcaccgacga
     4441 gatgagcgcc g
//
LOCUS       DBNGNK010000023         3210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1937718,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000023 DBNGNK010000000
VERSION     DBNGNK010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1937718"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..270
                     /locus_tag="AC150V_00620"
                     /pseudo
     CDS             1..270
                     /locus_tag="AC150V_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003876282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(382..1251)
                     /locus_tag="AC150V_00625"
     CDS             complement(382..1251)
                     /locus_tag="AC150V_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3761458.1"
                     /translation="MRLACADNTFRLVQPWESAVELIRLLEIPAVDVCLMGNRSHLRP
                     EEVRDDIPGCAGRIVAGLESRGLACSDLFCIPWTDFERYAPNHPDAEERKRAWELFEA
                     MLDLAARIGAPGLTMLPGIDWPGESHDDSLARSVAELGRRADAALARGVRFSIEPHLG
                     SVVQDPAEAKRLCAETPGVELTLDYSHFVYQGYTESAIEPLAEHARHFHARGAAERRM
                     QTALKDNVIDFERMVDVARAAGYDGDIGLEYLWIDWEGLNTCDTVSETILLRDRLLAA
                     IGGHPWSYPESTI"
     gene            complement(1264..1833)
                     /locus_tag="AC150V_00630"
     CDS             complement(1264..1833)
                     /locus_tag="AC150V_00630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761459.1"
                     /translation="MAAVTLVNVPSSLEPSLRQALSDVPHDTLVAGFGELRTGRFLDL
                     EQADWEAAVAGMRSAFLSARDAAAGLLDRGEPGRIVLLSTPPAVRPVHGASLPATAGG
                     FLTRAAQVAAAELGGSEITANVVVPGYMHDERFVDGIPAGRLAGPADVAAVVGFLASE
                     AASYVNGAVITVDGGFSITKTGGGSPLLA"
     gene            complement(1833..2609)
                     /locus_tag="AC150V_00635"
     CDS             complement(1833..2609)
                     /locus_tag="AC150V_00635"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761460.1"
                     /translation="MGRVDGKVALVTGASHERGIGRGIALALAREGADVACCDIAFED
                     AGEELAGELRELGRRAGFYRLDVTDRADVERVIEEVEADLGLIDIVASNAGTADWEEF
                     HEITDESWDRVVAVNLTGAFNVGQAAARAMVRHGKGGRIVFTSSVHAQMGFPKMAVYG
                     STKLAVASLADHMALELAEHGILVNHIGPGWVKSQLNDSSPDLQTEADEQATMELIPV
                     HRPSEPIEQGYAVVYLCSSEADYVTGEFLRVDGGLVVGKY"
     gene            complement(2609..>3210)
                     /locus_tag="AC150V_00640"
     CDS             complement(2609..>3210)
                     /locus_tag="AC150V_00640"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3761461.1"
                     /translation="RFAPFEPEVVLCLTGHPGDHDPVEARQILVDGLRQAAKVAGGHG
                     LTLGLEPLHREIYGHWSTIGDIPGTIELMEEVGEPNVMLLYDVYHLWDTDNVIEDTVR
                     HGDRIVKSVHVCDYRKETRNDFDRALPGDGVMDLPAIFGALDAGGVVGWFDLEIFSDD
                     GSFTDQDFEDSLWKEDPSEVVKRGKAGFDKAWAERKAPA"
BASE COUNT          473 a         1144 c         1116 g          477 t
ORIGIN      
        1 gccggcctgc tggcgacggt gaagggcctc ccgctcgcgt acaaccgcga cctgcaggag
       61 gacaagccgc ccgtgttcgc cacgcgcgga gagctgcggc tcgcactcca ggcgctgacc
      121 gtgctcgtgg ccggtctgga ggtcgatcga gcgaggctcg ccgccgccgc gtcggacgcg
      181 aacgtgctcg cgaccgacgc agccgaggcg ctcgtccgct ccggcgtccc cttccgcgac
      241 gcgcacgagc aggtcgccgc cgcagtgcgt gacgggacgt tcgagccgcc cgagacgccg
      301 agtcagcgcc ccgcaccggg ccccggcggc gtcaccgagg cgatcgccga cgcgcgacgt
      361 cgcttcggcg agggcctcta gctagatcgt cgactccgga tacgaccacg ggtggccgcc
      421 gatggccgcg agcagccggt cgcgcaggag gatcgtctcc gacaccgtgt cgcacgtgtt
      481 gaggccctcc cagtcgatcc acaggtactc gagcccgatg tctccgtcgt agccggcagc
      541 ccgtgcgacg tcgaccatcc gctcgaagtc gatcacgtta tccttgagcg ccgtctgcat
      601 ccggcgctcc gccgcgccgc gggcgtggaa gtggcgcgcg tgctcggcga gcggctcgat
      661 cgcagactcc gtgtagccct ggtagacgaa gtgcgagtag tcgagggtga gctcgacgcc
      721 cggtgtctcc gcgcagagcc gctttgcttc cgcggggtcc tggacgacag agccgaggtg
      781 cggctcgatc gagaaccgga ctcctcgcgc gagcgccgcg tcggcgcgac gcccgagctc
      841 cgcgaccgag cgcgcgagcg agtcgtcgtg gctctcaccg ggccagtcga tgcccggcag
      901 catggtgagc ccgggtgcac cgatgcgggc ggccagatcg agcatcgctt cgaagagctc
      961 ccatgcgcgc ttccgctcct cggcgtcggg gtggttgggc gcgtaccgtt cgaagtcggt
     1021 ccacgggatg cagaagagat ccgagcaggc gagtccgcgg ctctccagac ccgcgacgat
     1081 tcggcctgcg cagcccggga tgtcgtcgcg gacctcctcc ggccgcaggt gcgagcggtt
     1141 gcccatcagg cacacgtcga cggcgggaat ctcgagcagg cgaatcagct ccaccgcgct
     1201 ttcccacggc tgcacgagcc ggaacgtgtt gtcggcacag gcgagccgca tgcgtccagc
     1261 cggctacgcg agcagcgggc tgcccccacc ggtcttcgtg atcgagaagc cgccgtcgac
     1321 cgtgatcacg gcgccgttca cgtacgaggc cgcctcggag gcgaggaaac cgacgactgc
     1381 ggcgacgtcc gcgggcccgg ccaggcggcc cgccggtatc ccatcgacga agcgctcgtc
     1441 gtgcatgtag ccgggaacca cgacgttggc ggtgatctcg cttccgccga gctcggccgc
     1501 ggccacctgc gccgcgcggg tgaggaagcc gcccgcggtc gcagggagcg acgcgccgtg
     1561 caccggacga acggccggcg gcgtcgacag cagcacgatg cggccgggct cgccgcggtc
     1621 gagcagtccg gcggccgcgt cgcgcgcgga gaggaacgcg cttcgcatcc ccgcgacggc
     1681 cgcttcccaa tcggcctgct cgaggtcgag gaaacgtccc gtgcgcagct cgccgaaccc
     1741 ggccaccagg gtgtcgtgcg gtacgtcgga cagtgcctgg cggagactcg gctcgagcga
     1801 cgacgggacg ttgacgagcg tgacggcggc catcagtact tccccaccac gaggccgccg
     1861 tcgacgcgca ggaactcacc cgttacatag tcggcctctg acgagcagag gtagacgacc
     1921 gcgtagccct gctcgatcgg ctccgacggg cggtgcacgg ggatcagctc catcgtcgcc
     1981 tgctcgtcgg cctcggtctg caggtcgggc gagctgtcgt tcagctgcga cttcacccag
     2041 ccgggcccga tgtggttgac gaggatgccg tgctcggcga gctcgagcgc catatggtcg
     2101 gcgagcgagg cgacggcgag cttcgtcgag ccgtacacgg ccatcttcgg aaaccccatc
     2161 tgcgcgtgga cggaggacgt gaacacgatg cggccgccct tcccgtggcg gaccattgcc
     2221 cgggctgcgg cctgcccgac gttgaaggcg ccggtcagat tgacggcgac cacgcgatcc
     2281 cacgactcgt cggtgatctc gtgaaactcc tcccagtcgg ccgttcccgc gttcgacgcg
     2341 acgatgtcga tcagcccgag gtcggcctcc acttcctcga tcacgcgctc gacgtcggcg
     2401 cggtcggtca cgtcgaggcg gtagaagccc gcccgccggc ccagctcgcg tagctctccg
     2461 gcgagctcct cgccggcgtc ctcgaacgcg atgtcgcagc aggcgacgtc agctccctca
     2521 cgggcgagcg cgagggcgat gccgcggccg atcccccgct cgtgtgaggc accggtgacg
     2581 agagcgacct tgccgtcgac gcgtcccatc aggcgggcgc cttcctctcc gcccaggcct
     2641 tgtcgaaacc ggccttgccg cgcttgacga cctcgctcgg gtcctccttc cagagggagt
     2701 cctcgaagtc ctgatcggtg aacgatccgt cgtccgagaa gatctcgagg tcgaaccagc
     2761 cgaccactcc gcccgcgtcg agggcgccga agatcgccgg caagtccatc acgccgtcgc
     2821 cgggcagcgc ccggtcgaag tcgtttctcg tctccttgcg atagtcgcaa acgtggacgc
     2881 tcttcacgat ccggtcgccg tggcggacgg tgtcctcgat cacgttgtcg gtgtcccaga
     2941 ggtgatagac gtcgtagagg agcatgacgt tcggctcgcc gacttcctcc atcagctcga
     3001 tcgtccccgg gatgtcgccg atcgtcgacc agtgcccgta gatctcccgg tggagcggct
     3061 cgagaccgag cgtgagcccg tgcccaccgg cgaccttcgc cgcctgcctg aggccgtcga
     3121 cgaggatctg ccgcgcctcg acggggtcgt gatcgcccgg gtggccggtg aggcagagga
     3181 cgacctcggg ctcgaagggc gcgaaccgat
//
LOCUS       DBNGNK010000024         5263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_75454,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000024 DBNGNK010000000
VERSION     DBNGNK010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_75454"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..622)
                     /locus_tag="AC150V_00645"
     CDS             complement(143..622)
                     /locus_tag="AC150V_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761462.1"
                     /translation="MNETKAKLEASCYLAVGVVKVRGELIRTPTYADRQGLALGLACA
                     SLLGEVQARIDALESKPPAPGAADTPKRCQVVRFRLRSEKKSGRWMTEVVETGVKPRI
                     SATCRTTGDGLELRLRRANAGVLRDAVGEQLALTVRRSKAAPPPAQGAKLTLTVTQK"
     gene            1090..2049
                     /locus_tag="AC150V_00650"
     CDS             1090..2049
                     /locus_tag="AC150V_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3761463.1"
                     /translation="MDAIVVDGLEKTYGKDVRALDGIRFSVQEGEVFGLLGPNGAGKS
                     TTVRCLVTLTRADAGDAWVAGHHVEREPGLVRRSIGYVPQASGVDREATGRENLVLQA
                     RLQGMPSSDAARRSGGLLEGFGLSEKADAQVKTYSGGQKRRLDIAMGLVHRPQVLFLD
                     EPTTGLDPEARAAMWDELSRLAAAETLTILLTTHYLEEADRLAERIAIVSRGRIVVEG
                     KPEELKHALRGDAVTLEVGDDGAVRAVELLEKLAGVSEIIRDGRRVHTRVDSGAQAVP
                     AILSALEGGGVTVAAVTVARPSLDDVYLHYTGRDFRADDEAGA"
     gene            2046..2816
                     /locus_tag="AC150V_00655"
     CDS             2046..2816
                     /locus_tag="AC150V_00655"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761464.1"
                     /translation="MTTLRQTLYLCGRVLRNLSRQPIWIVVMVVQPMFWLLLYSQLFR
                     RITELPGFGTSSYIDYLTPGVAVMTAFFSGSWAGMGMIEDLDRGVLERFLTTPARRSA
                     VVFGRILESAIVATLQAVLILVTGLALGATNGGLTGWLVILLAAFLVAAGFAGLSHGV
                     ALLTRQEASMIAVAQFIGLPLLFFSTLLIARELIPGWMRSLSLLNPVEWAVRAARGEA
                     LPGTAWDEMGLFIVLLAAFAAAAGGFATWSFRAYQRTL"
     gene            2836..3213
                     /locus_tag="AC150V_00660"
     CDS             2836..3213
                     /locus_tag="AC150V_00660"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3761465.1"
                     /translation="MSVDLAAAVERYNAAWNEQQLDVIRSLHAPDIVFHNHTADERVA
                     GEADVAAHIAGIFERWPDLRFSGRSLYVGPAFVISEWTATATDDQGRRLEWDGVDIFP
                     FVDGKIARKDVYSSSATPRVLAG"
     gene            complement(3210..3902)
                     /locus_tag="AC150V_00665"
     CDS             complement(3210..3902)
                     /locus_tag="AC150V_00665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011890282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3761466.1"
                     /translation="MPEGERVLWITGASSGIGAATARHAAADGWSLVLSSRSEDKLEA
                     LAAELGEDHVVPHPCDVTDWEQQQSAVEAACEKFGRIDAVFANAGFGGQRSFLGDTPE
                     YWREMVLTNVFGAALTIRASIPALKESRGHLLLTGSVAGRRPVAGSLYSATKHAVHAM
                     AESLRQELDGSGVRVTVIAPGMTDTPFFAERPLEEALESEDIARAVMFALSQPPHVDV
                     NEVLVRPTAQTL"
     gene            complement(3915..4775)
                     /locus_tag="AC150V_00670"
     CDS             complement(3915..4775)
                     /locus_tag="AC150V_00670"
                     /inference="COORDINATES: protein motif:HMM:NF018677.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HIR3761467.1"
                     /translation="MSQCCADLARAASLPLGGTATRADRWLLLQVDGAWGRDALADST
                     LPEGAKARLQGWLAGTPQSRVLFIRRPRRSSESPEHLAFLVDTDETGGRVRRLRLFAY
                     EELAEVDLDGAGEEIEGPLVLVCTHGRRDQCCARRGVPVYEALSAHVPGELLWQSSHQ
                     GGHRLAANVIALPHAVQLGQVEPADAGPVASALLAGRIPLDQYRGRTLYDPIAQAADV
                     AVRLQRGLDRVEDVSVLGSEDGVVSLRAAGREIEVEVTEEEGPYVPASCGAAPEPSVR
                     LSVGAGAGGG"
     gene            complement(4772..>5263)
                     /locus_tag="AC150V_00675"
     CDS             complement(4772..>5263)
                     /locus_tag="AC150V_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761468.1"
                     /translation="RFWDALDDDVRHHGPPAVLLTIFWHARSAQAVLDRYQGATVWAF
                     EPARALVSERTQVTDTFLAGDVLPGGAIAYDAGREGEVVLWLPTHAALVVGDVVLGAG
                     GGEARLSPESWLAKGQTHDDVRRALEPVLGLPIELLLVTHGEVIATDAVGALRRALNG
                     GST"
BASE COUNT          774 a         1860 c         1809 g          820 t
ORIGIN      
        1 tcggaacgct cgtgcccgtc ggagaatcgc ctgccgggtt cccggagcgg ctggtctgga
       61 tcaccgacaa cgcctactac ctcaaggggg cgtgaccctt cgcacgcgca ccgcgtccgt
      121 ctgaacgaac ctcggcggcg ggctacttct gcgtcacggt cagggtcagt ttggcgccct
      181 gagctggcgg cggcgcagcc ttcgaccggc gcacggtcag ggcgagctgc tcgccgaccg
      241 cgtcccgaag caccccggca ttcgcgcggc ggagccgcag ctcgagcccg tctcccgtgg
      301 tcctgcaggt cgcgctgatt cgaggcttga cgcccgtctc gaccacctcg gtcatccacc
      361 gcccgctctt cttctcggag cggagccgga agcgcacgac ctggcaccgt ttcggtgtgt
      421 ctgcggctcc cggcgccggc ggcttgctct cgagggcgtc gatccgcgct tgcacctctc
      481 cgagcaggct cgcacatgcg agcccgagcg cgagaccctg tcgatccgca taggtgggcg
      541 tgcggatgag ctctccccga accttcacga cgcccacggc caggtagcag cttgcctcga
      601 gcttcgcctt ggtctcgttc aacttcttga gccgcctcgc cttctccacg agcgcgagcc
      661 cgacggaggc gacgtcgtcg agcgaggccc cgcggaaccc gccaggagag cacgaacgac
      721 tgatcgttcg tttcgttgtc gaacgtgagc gagaactggc cgtcggacac cttgccggtg
      781 cacgcgttcg cggggcaccc gacgacgggc cccgcacccg aactccatcg cccgccggga
      841 agcttcccgg aagcgtcgcg ccactgccgc tcgacggtcg tctgctcgag ccgccactcg
      901 aagctcgtct ctgcgccggg cgccactccg tcaacgagga agaaccacgc cgcaccggcg
      961 atggcgacgg cgacggcgac ggcgacgagc agagactgtc tgggctttcg tcccgacgtc
     1021 gaggccacag atcggcgacc gtatcacggc agtgttcccg cgccgctgca cgtcgctacg
     1081 ctccggcggg tggacgcgat cgtcgtcgac gggctcgaga agacatacgg gaaagacgtc
     1141 cgcgcactcg acggcatccg tttctcggtt caggagggtg aggtgttcgg gctgctcggc
     1201 ccgaacggcg ccggcaagtc gacgacggtc cgctgcctcg tcacgctgac gcgggccgac
     1261 gcaggggacg cgtgggtcgc cggccaccac gtcgaacgcg agcccggcct ggtacgtcgc
     1321 tctatcggct acgtgccgca ggcctccggc gtcgaccggg aggcgacagg acgcgagaac
     1381 ctcgtgctcc aggcccgtct ccagggaatg ccgtcgtccg atgcggcccg gcgctccggc
     1441 gggctgctcg aaggcttcgg tctgagcgag aaggcggacg cacaggtcaa gacgtactcg
     1501 ggtgggcaga agcgccggct cgacatcgcg atgggactcg tccaccgccc gcaggtgctc
     1561 ttcctcgacg agccgactac cggtctcgac ccggaggcgc gggccgcgat gtgggacgag
     1621 ctgtcgcgtc tcgcggcagc ggagaccctc accatcctgc tcaccacgca ctacctcgag
     1681 gaggccgatc ggctggccga gcgcatcgcg atcgtcagcc gtgggcgtat cgtcgtcgag
     1741 gggaagccgg aggagctcaa gcatgcgctg cgcggcgatg cggtgacgct cgaggtcggc
     1801 gacgacggcg ccgtgcgtgc cgtagagctt ctcgagaagc tcgcgggagt ctccgagatc
     1861 atccgggacg gccggcgcgt ccacacccgg gtcgactccg gcgcgcaggc cgtgcccgcg
     1921 attctctccg cgctcgaggg cggcggggtg acggtggctg cggtgacggt tgcgcggccg
     1981 tcactcgacg acgtctacct ccactacacg ggccgcgact tccgcgccga cgacgaggcc
     2041 ggcgcgtgac gactctccgc cagacgctct acctctgcgg ccgcgtgcta cggaacctct
     2101 cccggcagcc gatctggatc gtcgtgatgg tggtgcagcc gatgttctgg ctgttgctct
     2161 acagccagct ctttcggcgg atcaccgagc tgccgggatt cggcacctcg tcgtacatcg
     2221 actacctgac gcccggcgtt gccgtgatga cggcgttctt ctctggttcc tgggcgggaa
     2281 tgggaatgat cgaggatctg gaccgcggcg tcctcgagcg attcctgacg acgccggcgc
     2341 gccggagcgc cgtcgtgttc gggcgaatcc tcgaatctgc gatcgtggcg acgctgcagg
     2401 cggtgctcat tctcgtcacc gggctggcac tcggcgcgac gaacggaggg ctgacgggct
     2461 ggctcgtcat cctcctcgcc gcgttcctcg tggcggcggg cttcgccggg ctgtcgcacg
     2521 gcgttgcgct gctcacgcgg caggaggcct cgatgatcgc cgtcgcccag ttcatcgggc
     2581 ttcctctcct cttcttctcg accctcctga tcgcgcgtga gctgatcccc ggctggatgc
     2641 ggtcactctc gttgctcaac ccggtcgagt gggccgtccg cgcggcgcgc ggggaggcct
     2701 tgccgggtac ggcttgggac gagatgggcc tcttcattgt gcttctcgcc gcgttcgcag
     2761 ccgctgccgg agggttcgcg acgtggagct tccgcgccta ccagcgcact ctgtgacatc
     2821 cgatacgatc gccggatgag cgtcgatctc gccgccgccg tcgagcggta caacgctgcc
     2881 tggaacgagc agcagctcga tgtgatccgg agtctgcacg cgccggacat cgtcttccac
     2941 aatcacacgg ccgacgagcg ggtggccgga gaagcggatg tggcagcaca catcgccggc
     3001 atcttcgagc gctggcccga ccttcggttc agcgggcgct cgctctacgt cgggccggcg
     3061 ttcgtgatca gcgagtggac tgcgaccgcg acggacgacc aggggcggag actcgagtgg
     3121 gacggagtcg acatcttccc gttcgtcgac ggaaagatcg cccgcaagga cgtctactcc
     3181 agctctgcca cgccgcgggt gctcgcgggc tagagcgtct gggccgtcgg ccgcacgaga
     3241 acctcgttca catctacatg cgggggctgt gagagcgcga acatcacggc gcgcgcgatg
     3301 tcctccgact ccagcgcctc ctcgagcggt cgctcggcga agaacggcgt gtcggtcata
     3361 cccggtgcga tgacggtcac ccgcacgccg cttccgtcca gctcctggcg aagcgactcg
     3421 gccatcgcgt gcacggcgtg cttcgtcgcc gagtagaggg agcccgccac aggacggcgc
     3481 ccggcgacgg agccggtgag cagcaggtga ccgcgcgact ccttcagagc cgggatcgaa
     3541 gcgcggatcg tcagagccgc cccgaacacg ttcgtcagca ccatctcgcg ccagtactcc
     3601 ggtgtgtcgc ccaggaagct cctctggccg ccgaagcccg cgttggcgaa gaccgcgtcg
     3661 atccgcccga acttctcgca ggcggcctcg accgccgact gttgctgctc ccagtcggtg
     3721 acgtcgcacg ggtgcggaac gacgtgatcc tccccgagct cggccgcaag agcctcgagc
     3781 ttgtcctcgg agcgagagga gagcacgagg ctccacccgt ccgccgcggc gtgccgtgcc
     3841 gtggcggcgc cgattccgga ggaggccccg gtgatccaga gcacgcgttc gccttctggc
     3901 atgcgccccc gactctatcc gccgcccgca ccggccccga cggacaggcg tactgacggc
     3961 tcgggtgccg caccgcagct cgcagggacg tagggcccct cctcctcggt gacctccacc
     4021 tcgatctcac gcccggcagc gcggagggag acgacgccgt cttcgctccc cagcacggat
     4081 acgtcttcga ctcgatcgag cccccgctga aggcgcacgg cgacgtcggc cgcctgggcg
     4141 attgggtcgt agagggtccg cccgcggtac tgatcgagcg gtatgcggcc ggcgaggaga
     4201 gccgacgcaa cggggcccgc gtccgcgggc tcgacctgcc cgagctggac ggcgtggggc
     4261 agcgcgatga cgttcgcggc gagccggtgg ccgccctgat gggacgactg ccagagcagc
     4321 tcgccgggca cgtgggcgga caatgcttcg tacacgggca cgccgcggcg cgcacagcac
     4381 tggtcacggc ggccgtgggt gcagacgagg acgagcggtc cctcgatctc ttctccggcg
     4441 ccgtcgaggt cgacttcggc gagctcttcg tacgcgaaca gccggagtcg tcgcactcgg
     4501 ccgcccgtct cgtcggtgtc gacgagaaag gcaagatgtt cgggcgactc ggacgatcgt
     4561 cgcgggcggc ggatgaagag cacgcgagat tgcggggttc ctgccagcca tccctgcagc
     4621 cgcgccttgg ccccttcggg gagcgtgctg tcggcaaggg cgtcccgccc ccaggcgccg
     4681 tcgacttgga gcaggagcca ccgatcggcc cgggtcgccg ttcccccgag cggcagcgac
     4741 gccgcccggg cgagatctgc acaacactga ctcatgtcga gccgccgttc agcgcacggc
     4801 gaagggctcc cacggcgtcc gtcgcaatga cctctccgtg cgtgaccagc agcagctcga
     4861 tggggagacc gagcacgggc tcgagcgcac gccgaacgtc gtcgtgcgtc tgccccttcg
     4921 ctagccacga ctcgggcgac agccgagcct cgccgccacc cgctcccaga acgacgtctc
     4981 cgacaacgag cgcagcgtgg gtcgggagcc acaggacgac ttcgccctcc ctccccgcgt
     5041 cgtacgcgat ggctccgccc ggcagcacat caccggcaag gaacgtgtcg gtcacctgcg
     5101 tccgctcgga aacgagagcg cgggcgggct cgaacgccca cacagtcgcg ccctgatagc
     5161 ggtcgaggac tgcctgggcg ctgcgcgcgt gccagaagat cgtgagcagc acggcaggtg
     5221 gtccgtggtg ccggacatcg tcgtcgagcg cgtcccagaa ccg
//
LOCUS       DBNGNK010000025         3510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1508003,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000025 DBNGNK010000000
VERSION     DBNGNK010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1508003"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="AC150V_00680"
     CDS             complement(<1..937)
                     /locus_tag="AC150V_00680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3761469.1"
                     /translation="MANDAFIVDAVRSPIGKRNGSLAGIRADDLAAQVLNGLADRIDL
                     DPGEVEDVQMGCVTQVGEQALNVGRVAVLVAGWPESACATTVDRQCGSSMQAAFNASA
                     AVQAGHLDVVVAAGVEHMTRVPMGSNLGEQGWSVLNEKLGERWPIVPQGISAEVIAEE
                     WGIGREQLDEYSLESHRRAVAATDEGRFEREIVPIEVGAAGAAVVFAVDETPRRETSL
                     EKLATLEPAFKPDGVVTAGNSSSIVDGAAAMLVTSGAAAERLALAPRARFVSFGLSGV
                     DPYRMLHGNPQACARALEKAGLSWDDIAVIEVNEAF"
     gene            complement(927..2258)
                     /locus_tag="AC150V_00685"
     CDS             complement(927..2258)
                     /locus_tag="AC150V_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3761470.1"
                     /translation="MRPGTHYTRSGDVNIAYQVVGEGPLDLLFVHGWISNVEYGWEVP
                     PLARFLRRLSSFSRLILFDRRGTGLSDRVPASELPTLEERMDDVRAVLDAAGSERAAI
                     FGASEGGTLSLLFAATYPERTEALITFGIFAKRLRSDDYPWAPTLEDREAEAREIREE
                     WGTLPSIAHYAPSADPAFVERLAAFFRMSASPGAAEALLWMNTSMDVRHVLPAVHVPT
                     LVMHRSGDLTSHIDEGRWIASRIPGARFVELPGPDHLPYAGDQDAVLDEVEEFLLGAR
                     RGPVPERVLTTVLFTDIVGSTERAAAIGDRRWRTLLTEHHEVVRAELERWCGREVTTA
                     GDGFLATVDGPARAVRCAQAIGHVVRSLGLKVRAGIHTGEVELLGDDVGGIAVHTGAR
                     IATLAGPGEVLVSSTVTDLVAGSGLEFEDRGSHRLEGVPGDFRIYAVAGGE"
     gene            2283..>3510
                     /locus_tag="AC150V_00690"
     CDS             2283..>3510
                     /locus_tag="AC150V_00690"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3761471.1"
                     /translation="MATTVREQRILVAGEWVETGDWLEIRSPYDGSSVGRVAKAGAAE
                     TRAAIDAAERAMADPLPAHKRAEIRVRVAGALGRRADEAARQISAEAGKPLKAARVEV
                     ARAMSTYTMAAVEARKLAGEMVPMDGSQAGEGKLAFTLRLPIGVVGAISPFNFPLNLV
                     AHKIAPALAAGCAVVLKPASQTPLSAFLLAELETEAGLPPGWLNVLAGPASEIGDVLV
                     EDERVKLITFTGSGDVGWKIAERAPKKRVNLELGNATPVIVEPDADIADTAARLAANA
                     FSFAGQSCISVQRIYVHRDVFDDFKAQFVPLVDALVVGDPGAEDTDVGPLISTSERDR
                     VASWIDEASSGGGTVLTGGELDGELLRPTVVEAPPADARLACEEAFGPVCTLSPYDDL
                     AEAISLANGTRLGLQAG"
BASE COUNT          541 a         1249 c         1212 g          508 t
ORIGIN      
        1 cgaacgcctc gttcacctcg atcacggcta tgtcgtccca gctcaggccc gccttctcga
       61 gcgcccgcgc acacgcctgc gggttcccgt gcagcatgcg gtacggatcg acgccggaca
      121 gcccgaatga gacgaagcga gcgcgcggcg cgagtgcgag ccgttccgcc gcggcgccgc
      181 tcgtgacgag catcgcagcg gcgccgtcga cgatggagct ggagtttccg gccgtgacga
      241 cgccgtccgg cttgaaggcg ggctcgagcg tcgccagctt ctccagcgac gtctcacggc
      301 gtggtgtctc gtcgacggcg aagaccacgg cagcccccgc cgcaccgacc tcgatcggca
      361 cgatctcacg ctcgaagcgt ccctcgtcgg tcgccgctac tgcccgccga tgagattcga
      421 gcgagtactc gtcgagttgt tcgcggccga tgccccattc ctcggcgatc acctcggcgg
      481 agatcccctg tggcacgatc ggccagcgct cgccgagctt ctcgttcagc accgaccagc
      541 cctgttcgcc gaggttcgat cccatcggca cgcgcgtcat gtgctcgacg cccgccgcga
      601 cgacgacgtc gaggtgaccg gcttggacgg ccgcagacgc attgaacgca gcctgcatcg
      661 acgagccgca ctgccggtcg acggtcgtcg cgcacgccga ctccggccag cctgcgacga
      721 gcacggccac gcgtccgacg ttcagggcct gctctccgac ctgggtgacg cagcccatct
      781 gcacgtcctc gacctcgccg ggatcgagat cgattcggtc ggccaggccg ttcagcacct
      841 gcgcggcgag atcgtcggcg cggatacccg ccagcgagcc attgcgcttc ccgatcggac
      901 tgcgcaccgc gtcgacgatg aacgcatcat tcgccaccag cgacagcata gatgcggaag
      961 tcgcccggca cgccctcgag ccggtggctg ccgcggtcct cgaactccag accggacccc
     1021 gcgacgaggt cggtgacggt gctcgagacg agcacctcgc ccggcccagc gagcgtcgcg
     1081 atccgcgcgc ccgtgtggac ggcgattccg ccgacgtcgt caccgaggag ctcgacctcg
     1141 ccggtgtgga tgccggcccg aaccttcagg ccgagcgaac gcacgacgtg cccgatcgcc
     1201 tgcgcgcagc ggacggcgcg agccggcccg tcgacggtgg cgaggaagcc gtcgcccgcg
     1261 gtggtcacct cgcggccgca ccagcgctcg agctcggccc ggacgacttc gtggtgctcg
     1321 gtgaggagcg tgcgccagcg cctatcgccg atcgctgcag cccgctcggt tgagccgacg
     1381 atgtcggtga agagcacggt ggtcagcact cgctcgggca cgggcccgcg ccgcgcgccc
     1441 aaaaggaact cctcgacctc gtcgagcacg gcgtcctggt cgcccgcgta gggcaggtga
     1501 tccgggccgg gaagctcgac gaaccgtgca ccggggatgc ggctcgcgat ccatcgcccc
     1561 tcatcgatgt gggaggtgag atcgccgctc cggtgcatga cgagcgtcgg cacgtgtacg
     1621 gcaggcagaa cgtggcgcac gtccatcgac gtgttcatcc agagcagtgc ttcggccgcc
     1681 ccggggctgg cgctcatccg gaagaacgcc gcgagccgct ccacgaacgc cggatcggcg
     1741 ctgggcgcgt agtgggcgat gctgggcagc gtgccccact cctcccggat ctcgcgcgct
     1801 tcggcttcgc gatcctccag cgtcggcgcc catgggtagt cgtcgctccg caaccgcttg
     1861 gcgaagatgc cgaacgtgat gagggcttca gtccgctctg ggtaggtggc ggcgaagaga
     1921 aggctcaacg tccccccttc ggaggcgccg aagatcgcgg cgcgctcgct gccggcggcg
     1981 tcgagaacgg cccgaacgtc gtccatccgt tcctccaacg tgggcagctc gctggccgga
     2041 acccggtcgg acaggccggt gccgcgccgg tcgaacagga tcagtcgact gaacgacgag
     2101 agccgccgca ggaaccgggc caacggcggc acctcccagc cgtactcgac gttggagatc
     2161 cagccgtgga cgaagaggag gtcgagcggc ccctccccga cgacctggta ggcgatgttg
     2221 acatcgccgc ttctcgtgta gtgggtcccc ggacgcacgg cccagaggat tacactccgc
     2281 gcatggcgac gacggtccgg gagcagagga ttctcgtcgc cggcgaatgg gtcgagacgg
     2341 gcgactggct cgagattcgc tccccctacg acggatcttc ggtcggtcgc gtcgccaagg
     2401 ccggtgcggc ggagacccga gccgcgatcg acgcggccga gcgcgcgatg gcagacccgc
     2461 tgccggccca caagcgggcc gagatccggg tgcgcgtcgc gggcgcactc gggcggcgtg
     2521 ccgacgaagc ggcgcggcag atcagcgccg aagccgggaa gccgctcaag gccgcccgtg
     2581 tcgaggttgc acgggcgatg tcgacctaca cgatggctgc ggtggaggcc cgaaagcttg
     2641 ccggcgagat ggtgccgatg gacggctcac aggccggcga gggcaagctc gccttcacgc
     2701 tgcgcctccc gatcggcgtg gtcggtgcga tttcgccctt caacttcccg ctcaacctcg
     2761 tcgcccacaa gatcgcacct gcactcgccg ccggctgcgc cgtcgtgctc aaaccggcct
     2821 cgcagacccc tctgtcggcg ttcctgctgg cggagctcga aacggaggcg gggcttccgc
     2881 ccggctggct caacgtcctc gccggaccag cgtcagagat cggcgacgtg ctcgtcgagg
     2941 acgagcgggt gaagctgatc accttcaccg ggtcgggcga cgtgggctgg aagatcgccg
     3001 agcgggcgcc gaagaagcgg gtcaacctcg agctgggaaa cgcgactccc gtgatcgtgg
     3061 agcccgacgc cgacatcgcc gacaccgcgg cgcgattggc ggcaaacgcc ttctccttcg
     3121 caggccagag ctgcatctcg gtgcagcgga tctacgtcca ccgggatgtc ttcgacgact
     3181 tcaaggcgca gttcgtccca ctcgtggacg cgctcgtggt cggcgacccc ggtgcggagg
     3241 acacggacgt cggcccgttg atctcgacga gcgagcgcga ccgcgtcgcc agctggatcg
     3301 acgaggccag ctccggcggc ggcaccgtcc ttaccggcgg agagctcgac ggcgagctgc
     3361 tgcggcctac ggtcgtcgag gcgcctcccg ccgacgcgag gcttgcctgc gaggaggcgt
     3421 tcggcccggt ctgcacgctc agcccttacg acgatcttgc cgaggcgatc tcgctcgcga
     3481 acggcacacg gctcgggctc caggcaggga
//
LOCUS       DBNGNK010000026         3540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_146901,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000026 DBNGNK010000000
VERSION     DBNGNK010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_146901"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /gene="mscL"
                     /locus_tag="AC150V_00695"
     CDS             complement(<1..341)
                     /gene="mscL"
                     /locus_tag="AC150V_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011434546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HIR3761472.1"
                     /translation="MLQDFRDFLMRGNLVVLAIAFVMGVAFAALVTSFVDDLIMPIIA
                     MIFGKPSFNDLTFTINDAIFRYGSFITAVITFVTIAAAIFFFVVKPYDAYKARQPDEE
                     EEDSDEERRHK"
     gene            complement(545..1228)
                     /locus_tag="AC150V_00700"
     CDS             complement(545..1228)
                     /locus_tag="AC150V_00700"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3761473.1"
                     /translation="MPRFRWLLLGALAVVALLWLAPTVTAFLSDIDIESLSRPYSLIF
                     AFVAFDAVIPIFPSESLITTASNLAAQDGTALNIFYVFAAAAAGAIVGDSLLYWLSRT
                     VLRDVMSTQLDKARKNPKITRALQVLGETAPLLIVAGRFVPGMRFVVGATMGISHYPY
                     PRFLLWDAIGGTLWAGYTCFFSYLVASAVDDKPVLSIAVSVVVTTALLGLLYIPVKRR
                     WDEAGQAST"
     gene            complement(1231..2946)
                     /locus_tag="AC150V_00705"
     CDS             complement(1231..2946)
                     /locus_tag="AC150V_00705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR3761474.1"
                     /translation="MTPGRILPLVGELRRYERSWLTPDLIAGVTVWALLVPEAIAYAS
                     IAGMPPEAGLYTALPAVILYALFGTSRQLFVGPSSAVALLSASTVAAVVGSGSEDFFA
                     ATSALAIMVGLVCIAAGVLRLGFVSNFLSKPILDGFIAGLALVIVVGQAPKLFGVEGG
                     DGNFFEELWALVSELGETNRNTLLVGAGSLALLFALERFVSRLPGALAVVLLSILAVS
                     AFDLEEEGIEIVGEIPTGLPELAFPSVGGPVLAGLFGGAIGIVLVAYAETIAAARKYA
                     FKHREEIDPDQEFIALGAANVGAGLFQGFTVDGSLSKTAAADEAGQKTQVAGIVNAAL
                     ILLTILFLATLFENLAEATLGAVVIHAVWHLAVNPALWRRLWWLQRTEFTLALICALG
                     VMIVDVLPGLMFAVFVSLALLVYRAAYPRTAVLGLPEGAAVEDVRLRDVSEHPGDRTT
                     PGLIVYRFDAELFFANASTFTTEIDGLVRASDPPASMVLVDGEAITNIDITASDAVGE
                     LQAALADEGVTLAFARLRTPVREVMERTGLLDQVGEENLYETTTAGVAAFEQGRRPRP
                     GPATT"
     gene            complement(3092..>3540)
                     /locus_tag="AC150V_00710"
     CDS             complement(3092..>3540)
                     /locus_tag="AC150V_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761475.1"
                     /translation="RDRYNQPAQLALRINLLFHTSAQSFGQVMSMVEPRRAVAYHFFN
                     DADTRYDIYDGIRETYDGPLSMATDLMTWNITRDAVVERMAVSPDSDWEVAGPGEELA
                     PDRSRKPEYTKFILDGRLDVDDANRSWMQAFMQKHGLTPDDLKVGE"
BASE COUNT          643 a         1126 c         1221 g          550 t
ORIGIN      
        1 tccttgtgac gccgctcctc gtccgagtcc tcctcttcct cgtccggctg gcgggccttg
       61 tacgcgtcgt acggcttgac gacgaagaag aagatcgccg cggcgatcgt cacgaacgtg
      121 atcacggcgg tgatgaacga gccgtagcgg aagatcgcgt cgttgatcgt gaacgtgagg
      181 tcgttgaagc tcggcttacc gaagatcatc gcgatgatcg gcatgatcag gtcgtcgacg
      241 aacgaggtga cgagcgcggc gaacgccacg cccatcacga aggcgatcgc gagcacgacg
      301 agattgccgc gcatcaggaa gtcgcggaag tcctgcagca tggagagccc tcctgggtcg
      361 gttgaagggc gattcttact agtggagcgg acgcaggtcg tgagcggccg tgacaacggc
      421 gagcggcctg gcttctgcgg caggcgccga cctcacctgg agtctcggac agggtcatcg
      481 cgtaagcatc agtcttcggc ccagcagcat cgcttcgatc ggagcgcgcc ggtgtggccg
      541 atcctcaggt gctcgcttgc ccggcttcat cccagcggcg ctttaccggg atgtagagga
      601 ggccgagcag tgctgtggtg acaacgaccg agactgcgat cgagaggacg ggtttgtcgt
      661 cgactgcgct cgcaaccagg tacgagaaga aacatgtgta gccggcccac aacgtgccgc
      721 cgatcgcgtc ccacagcagg aagcgcgggt acgggtagtg gctgatgccc atagtcgcgc
      781 caacgacgaa gcgcatcccc ggaacgaagc ggccggcgac gatcagcagc ggagccgtct
      841 cgcccagcac ttggagcgcc ctcgtgatct tcgggttctt tctcgccttg tcgagctgcg
      901 tcgacattac gtcccggagc acggttcggg agagccagta gaggagcgaa tccccgacga
      961 ttgcgccggc cgccgctgcg gcgaagacgt agaagatgtt gagggctgtg ccgtcctgtg
     1021 cggccaggtt cgacgccgtg gtgatgagcg actcgctcgg gaagatggga atgacggcgt
     1081 cgaaggcgac gaacgcaaag atcagcgagt agggacggga aagactctcg atgtcgatgt
     1141 ccgacagaaa cgccgtcacc gtcggagcca gccacagaag ggcgacgact gccagcgcgc
     1201 cgagcaggag ccagcggaag cgtggcaacc tcacgttgtg gccgggccgg gccgtgggcg
     1261 acggccctgc tcgaaggcgg cgacgccggc cgtcgtcgtc tcgtagaggt tctcctcgcc
     1321 gacctggtcg agcagcccgg ttcgctccat cacctcgcgg actggcgtgc gcaggcgcgc
     1381 gaacgcaagg gtgacaccct cgtccgccag ggcggcctgc aactcgccga ccgcgtccga
     1441 tgcggtgatg tcgatattgg tgatcgcctc gccgtcgacg agcaccatcg aggccggtgg
     1501 atcgctcgct cgaacgagtc cgtcgatctc ggtcgtgaag gtgctggcgt tggcgaagaa
     1561 gagctcggcg tcgaagcgat agacgatcag gcccggcgtc gtccggtcgc ccggatgctc
     1621 ggatacgtca cgcagccgca cgtcctcgac ggcggcgcct tccgggagtc cgagaacggc
     1681 ggtacgcgga tacgcggcgc ggtagacgag cagcgccagc gagacgaaga ctgcgaacat
     1741 gagaccgggc aggacatcga cgatcatcac gccgagggcg cagatgagag ctagcgtgaa
     1801 ctccgtgcgc tgcagccacc agagccgtcg ccagagcgca gggttgacgg ccaggtgcca
     1861 gacggcgtgt atgacgactg cgccgagcgt cgcctcggcc aggttctcga acagagtggc
     1921 gaggaacagg atcgtgagca ggatgagcgc tgcgttgact atgccggcga cctgcgtctt
     1981 ctgtccggcc tcgtctgccg ctgccgtctt cgagaggctg ccgtcgaccg tgaacccctg
     2041 gaagaggccg gcaccgacgt tcgcggcacc gagcgcgatg aactcctgat cgggatcgat
     2101 ctcttcgcgg tgtttgaacg catacttgcg ggctgccgcg atcgtctccg cgtaggcgac
     2161 gagcacgatg ccgattgcgc ccccgaagag tcccgcgagg acggggccgc cgacagacgg
     2221 aaacgcgagc tcgggaagtc ccgtcgggat ctcgcccacg atctcgatgc cctcctcctc
     2281 gagatcgaag gcggagaccg cgaggatcga cagcaggacg accgccagcg cccccgggag
     2341 ccgagacacg aaccgctcga gcgcgaaaag gagcgcgagc gatccggcac ccaccaggag
     2401 cgtgttgcgg ttggtttctc ccagctcgct gaccagagcc cagagctcct cgaagaagtt
     2461 gccgtcgccg ccctcgaccc cgaacagctt cggcgcctgc ccgacgacga tcacgagcgc
     2521 caaccccgcg atgaacccgt cgaggatcgg cttcgagagg aagttggaca cgaaaccgag
     2581 gcggaggacg ccggctgcga tgcagacgag tccgaccatg atcgccagcg ccgaggtggc
     2641 cgcgaagaag tcctccgagc ctgagccgac gacggcggcg accgtcgagg ccgacaggag
     2701 cgcaacggcg gagctggggc cgacgaacag ctgccgggag gtgccgaaca gcgcgtacag
     2761 gatgacggcc gggagagctg tgtagagacc ggcctcgggc ggcattcccg cgatcgaggc
     2821 gtaggcgatc gcctccggga cgaggagcgc ccagaccgtg acgccggcga tcaggtccgg
     2881 cgtcagccag ctccgctcgt agcggcgaag ctcaccgacg agcggaagaa tccgtcccgg
     2941 cgtcacaccc aagccaacct atgtcctcga tctcgggtac ggtcgacccg gctccgcgag
     3001 gatccctgcc tccgcgaccc acccccggta acgtcgcctc cgaacggagt ggtcgtcatg
     3061 gctcctgagc tcctcccgct tgccctcgta gctactcgcc gaccttgagg tcgtccggcg
     3121 tgaggccgtg cttctgcatg aaggcctgca tccaggagcg gtttgcgtcg tcgacgtcga
     3181 ggcgcccgtc gaggatgaac ttggtgtact cgggcttgcg gcttcggtcc ggtgcgagct
     3241 cctcgcccgg ccccgcgact tcccagtcac tgtcaggcga gacggccatg cgctcgacga
     3301 ccgcgtcgcg ggtgatgttc caggtcatca ggtcggtcgc catcgagagc gggccgtcgt
     3361 acgtctcgcg gatcccgtcg tagatgtcgt agcgtgtgtc ggcgtcgttg aagaagtgat
     3421 acgcgaccgc gcgtcggggc tcgaccatgc tcatgacctg cccgaacgac tgcgccgacg
     3481 tgtggaacag caggttgatg cgtagcgcca gctgcgccgg ttggttgtac ctgtcccgca
//
LOCUS       DBNGNK010000027         2597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1723446,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000027 DBNGNK010000000
VERSION     DBNGNK010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1723446"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="AC150V_00715"
     CDS             complement(<1..797)
                     /locus_tag="AC150V_00715"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR3761476.1"
                     /translation="MAAAQVALRSEGFYVGQVDGVAGPLTKAAVLRFQSVRGLPADGK
                     IGSRTRRALGVLGRPLLGQRGLWVGRVGWDVSALEFALLGYGLAPAAVDGRFDEQTAA
                     AVRRFQRATGLIPDGVAGPRTFQAITPRLRAPANTAARHHDVKPGESFFSIAQRYHLS
                     PWRLARMNGYRLTGVLVPGARLRLPSGAKASPLAPNRNAVRAAINRWSSEYGVDPKLA
                     RALGWMESGFQQDVVSSVGAVGVMQLLPETWAWVDTMLIGEETPRTA"
     gene            complement(925..1425)
                     /locus_tag="AC150V_00720"
     CDS             complement(925..1425)
                     /locus_tag="AC150V_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3761477.1"
                     /translation="MPVGRNEFVSVMSAFPTGVAIVTTLEPDGTPRGLTTNAVTSVSA
                     EPPILLVCVARNSRTLPALLHTKRFVVNFMRDDHAELCALFASKADDKFVDVSWTAGG
                     GGIPILHEGAIAHAECATLEEHEVGDHVVVLGLVESAAPPDPDEIPIVYFRKEYASAP
                     APPGDD"
     gene            complement(1647..1719)
                     /locus_tag="AC150V_00725"
     tRNA            complement(1647..1719)
                     /locus_tag="AC150V_00725"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1684..1686),aa:Asn,seq:gtt)
     gene            1803..>2597
                     /locus_tag="AC150V_00730"
     CDS             1803..>2597
                     /locus_tag="AC150V_00730"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR3761478.1"
                     /translation="MPGLLESAALQLRVAKRVALRRGASLGERFGALDDRLVFVVGCP
                     RSGTTFLAGAIGSLPSFIDLGEVAPVKATVPDLVQQPVEEAARQLRRTLAVARRFGLV
                     GSVRCVEQTPEMAHLVDVAALAFPEARIVHIVRDGRDVACSLIARGWLSAARAGADDA
                     GVQYGAYTRFWVEPERATEFGAVSDARRAAWAWRRYVSAAREASAPVYELRYEDLVAD
                     PDAAAPRLAEYLDVDSVPLAAALHAAHGESVVDYGCGSGILAIVACR"
BASE COUNT          387 a          934 c          888 g          388 t
ORIGIN      
        1 tcggccgtac gcggcgtctc ctcaccgatc agcatggtgt ccacccaggc ccaggtctcg
       61 ggcaggagct gcatcactcc gactgcgccg acggacgaga ccacgtcctg ctggaatccc
      121 gattccatcc agcccaaggc gcgagcgagc ttcgggtcga cgccgtactc gctcgaccag
      181 cggttgatcg ccgcgcgaac cgcgttgcgg ttcggagcga gcggcgatgc cttggctccg
      241 ctcgggaggc ggaggcgggc gccgggtacg agcaccccgg taaggcggta gccgttcatg
      301 cgggccaggc gccacgggct caggtggtag cgctgcgcga tcgagaagaa gctctcgccg
      361 ggcttgacgt cgtgatgtcg cgccgcggtg ttcgcaggtg ctctcagccg gggcgtgatc
      421 gcctgaaacg tgcgcgggcc cgccacgccg tccggtatca gccccgtggc ccgctggaag
      481 cgacgcaccg ctgccgccgt ctgctcgtcg aaccggccgt cgaccgcggc cggcgccagc
      541 ccatatccga gaagcgcgaa ctcgagcgct gagacgtccc agccgacccg gccgacccag
      601 agcccacgct ggccgagcag gggccggccg agcacgccga gcgctcgccg tgtgcgcgac
      661 ccgatcttcc cgtctgccgg aagcccccga accgactgga agcggagaac agcggccttc
      721 gtcagcggtc cggcgacgcc gtcgacctgc ccgacataga agccctcgga ccggagcgcg
      781 acctgtgccg cggccacggc actcgatgtg cctccctctg ccaggggcgc agcgaggaga
      841 gccgcacatg cggttgcgag aaggatccgt cgcagcacgg acctctctgg ttcggcggca
      901 cccgctccgt tcctgcccgc tgcgctagtc gtctcccggt ggagccggcg cgctcgcgta
      961 ctccttgcga aagtagacga tggggatctc gtccgggtcc ggcggcgccg cagactcgac
     1021 caagccgagc acgacgacgt gatcgccgac ctcgtgctct tcgagcgtcg cgcactcggc
     1081 gtgagcgatc gctccctcgt gaagaatcgg gataccgccg ccgccggcag tccacgagac
     1141 gtcgacgaac ttgtcatccg ccttcgacgc gaagagggca cacaactcgg catgatcgtc
     1201 acgcatgaag ttcacgacga agcgcttcgt gtgcagcaga gccggcagcg tgcgcgagtt
     1261 gcgcgccacg cacaccagca ggatcggcgg ctcggccgag acgctcgtca ccgcgttcgt
     1321 cgtgagccct cgcggcgtgc cgtccggctc cagcgtcgtc acgatcgcaa cgcccgtcgg
     1381 gaaagcgctc atcaccgaca cgaactcgtt ccgcccgacg ggcatgtcga cagtctggca
     1441 ggtcaggacg ggagttgacg aggcgcggct agtctctacc gacggattcg ggaggacgcc
     1501 cgcgaggcgc tccgctcgtt cgaaaacgcg tccggccgag cagaaatccc catcgaggga
     1561 cacatactgc gagcccatcc gagctaaggc gtcgtcggcc cggggggaac ccctggttcc
     1621 cccgtgagcc catcctcacc aggaagctcc ccgggttgga ctcgaaccaa caaccctccg
     1681 gttaacagcc ggatgctctg ccaattgagc taccggggat cgcgcggcgg cgagattgta
     1741 gccgagcagg cggcgccacc tgccgcacca ccccgggcgc cgacggctgc aagaatgccg
     1801 cggtgccggg actcctcgaa tctgccgcgc tgcagctccg cgtggcgaag cgcgtggcac
     1861 ttcgccgggg cgcgagcctg ggcgagcggt tcggggcgct cgacgatcgg ctcgtcttcg
     1921 tcgtcggctg cccccgctcc ggcacgacgt tcctggccgg cgcgatcgga tcgcttccca
     1981 gttttatcga tctgggtgag gtcgcgccgg taaaggcgac tgtgccggac ctcgtgcaac
     2041 agccggtcga ggaggccgcc cgtcagctcc ggcgaacgct ggcggtcgcg cgccggttcg
     2101 ggctggtggg ctcggttcgc tgtgtcgagc agacgcccga gatggcccac ctcgtcgacg
     2161 tcgcggcgct cgcgtttccc gaggcgcgaa tcgtccacat cgtgcgagac ggccgcgatg
     2221 tcgcgtgctc gctgatcgcc cgcggctggc tcagcgccgc ccgtgccgga gccgacgatg
     2281 ccggcgtcca gtacggcgcc tatacgcgct tctgggtcga gccggagcgc gcaaccgagt
     2341 tcggcgcggt cagcgacgcc cgccgggcgg cgtgggcctg gcgccgctac gtctccgcgg
     2401 cccgggaggc gtccgcgccc gtatacgagt tgcgctacga ggatcttgtc gccgatccgg
     2461 atgccgccgc accaagactc gccgagtacc tcgacgtcga ctccgttcca ctcgcggctg
     2521 cgctgcacgc tgcccacggc gagtcggtcg tcgattacgg ctgcggctcc ggcattctcg
     2581 cgatcgtcgc ctgccgc
//
LOCUS       DBNGNK010000028         5462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_433839,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000028 DBNGNK010000000
VERSION     DBNGNK010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5462
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_433839"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1746
                     /locus_tag="AC150V_00735"
     CDS             <1..1746
                     /locus_tag="AC150V_00735"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761479.1"
                     /translation="EPPNLPPRQTVKHLYPLWREHRPLVILGLACALAFTFLSLLIPV
                     LIQRTIDDAIDTGDRQLLGTYLGAILAVAVLQFGVNFTRRYATARIGIGVEARLRSKL
                     YEGYLRFPRAFYDRHATGEVISRATNDLYPVRYFIGWGVIQSIQSSLMIVGALAVLAN
                     VNARLALLAALALPPIAVLTYVFAHRIFPISRRVQERKGNLTEATDEAVVGIEMVQAF
                     GREEDVRDRFSGRAAAVRGETMNQAGVEARYLPGLIFLPTLSIAAVLYFGGRDAIEGT
                     LTIGEFTLFVTLLLQLVWPLEALGWIFNLGQRAFAAASRGFGWLEGVAPLAAPEHPAP
                     LPDGPLAVRFESVHFSYGTGVEVLRGIDLDARPGEILAICGPTGVGKTSLLNLIPRFY
                     DPDAGRVLLGGVATDETSLDELRSAVALVTQKPVLFSVTLRDNLVAARPDAPWEVVLA
                     ASEAAGVEAFVSDLPHGYETMIGERGVNLSGGQRQRVALARALIADARVLVLDDPMSA
                     VDTQTERLLVENLRPAVAGRTVLIAGQRLSTILVADRAVVLEDGRITEQGTPGELLNR
                     GGAFAALFGDETVAA"
     gene            1760..3487
                     /locus_tag="AC150V_00740"
     CDS             1760..3487
                     /locus_tag="AC150V_00740"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761480.1"
                     /translation="MTRLWRYSAGRRRHIALVTSLAGISAAAPVAGWLLVGDAIDNGI
                     RAGDESRLTMDVIAYIGVNAVAWLLGSTTWLMLAGIGQDIVLAVRRDLFEHLTSLSLR
                     YFSEQKAGWIIARLTSDVDALSDVLTQGLTTLVVNTLTLVAAVAGLFLLDWRLGLVAL
                     LVLPPGTLITRWFQRRSHAAFAEVRTRIAAVTAQLAESVSGMAIVQAFNRERAFQSEF
                     EQLNDENRKANYVAQKVSSVFFPAVQLLGVVATVAVLLAGALLIDAGSLEIGTLVAAI
                     GLLQLVFQPLQELSELYGQVQSANAAMDKISSVLDAEQDIKDRPGAKPIGRVDGHLQL
                     DDVVFAYGEEPVIRGISIDVRTGGCIALVGESGGGKSTTARLIARFYDPLEGTIRVDG
                     KDLRDVKLRSYRRQLGVVLQDPFLFSGTIADNIRFARPDAADAEVRAAAEAVGVDRVA
                     ARFEDGLDHVVREGGAGLSAGERQLISIARALLADPRILILDEATSNIDRPTEILIER
                     AFDRLLAARTSIIIAHRLTTVRRADEILVVEHGRIVQRGIESELLAQEGPFRQLAHTL
                     EAASLKAAV"
     gene            complement(3495..4721)
                     /locus_tag="AC150V_00745"
     CDS             complement(3495..4721)
                     /locus_tag="AC150V_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3761481.1"
                     /translation="MTPEERVDRYAELVVRLGANVQRGQDVVVMAQVEHAEIARAVAR
                     QAYRVGASHVMSLYGDAHFRAALVEFGPEESLGWTPPYLLDWVKSWRETRPALISLTG
                     NPDLHLLSRFDPERVAKSEPIELRAAYLPLVTERLLNWVIVPAPNEGWATSVFGEPDV
                     ERLWGAVATAMRLDEADPVAAWTERAAVLGARSSTLAERRFDSIRFRGPGTDLTIGLL
                     PGASWLSALFTTQSGLQHLPNIPTEEVFTSPDRRRADGSVRSTYPLAVPGTGVLAENL
                     AMRFEGGKIVDVEATTGVETIKAQLATDANAPYLGEVALVDGTSRVRETGLVFHDTLF
                     DENATCHIAYGAGLPFTHADAAGLGPDELVELGINVSGVHTDFMIGGPDVEVDGLDAK
                     GAATPIIRDEAWLLDG"
     gene            complement(4756..5067)
                     /locus_tag="AC150V_00750"
     CDS             complement(4756..5067)
                     /locus_tag="AC150V_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761482.1"
                     /translation="MTVETESAFARAIQDHLDLKAKNSALDERLPIDTYSGEDLFENN
                     PLFKSEEQARIEETMDGAEAPAAVAAGPLSWPGEEAAEEGGAALDEALWGRSRDFDWG
                     D"
BASE COUNT          838 a         1889 c         1813 g          922 t
ORIGIN      
        1 gagccgccga atctgccgcc caggcagacc gtcaagcacc tctatccgct ctggcgcgag
       61 caccgcccgc tcgtgatcct cggcctggcc tgcgcgctgg ccttcacgtt tctctcgttg
      121 ctcatcccgg tgctgatcca gcgcacgatc gacgacgcca tcgacacggg tgaccgccag
      181 ctgctcggca cctatctcgg cgcgatcctc gccgtcgcgg tgctgcagtt cggagtgaac
      241 ttcacccgcc gctatgcgac ggcgcgcatc ggtatcggcg tcgaggcacg actgcgcagc
      301 aagctgtacg agggctatct ccgtttcccg cgcgcgttct acgaccgcca cgccacaggt
      361 gaggtgatct cgcgagcgac caacgacctc taccccgtgc gttatttcat cggctggggc
      421 gtgattcagt cgatccagag ctcgctgatg atcgtcggcg ctcttgcggt cctggcaaac
      481 gtgaacgcga ggctcgcgct cctcgccgcc ctggcgctgc ccccgatcgc cgttctgacg
      541 tacgtcttcg cgcaccgcat cttccccatc tcgcggcggg tgcaggagcg caagggcaat
      601 ctcaccgagg ccaccgacga ggccgtcgtc ggaatcgaga tggtgcaggc cttcggccgc
      661 gaggaggacg tccgcgatcg attctccggg cgcgccgccg ctgtgcgcgg cgagacgatg
      721 aaccaggcgg gggtcgaggc acgctacctc cccggcctca tcttcctgcc gactctctcg
      781 attgccgccg tcctctactt cggcggccga gatgccatcg agggcacgct gacgatcggg
      841 gagttcaccc tcttcgtcac gctgctgctc caactcgtct ggcccctgga ggcgctcggc
      901 tggatcttca acctcggcca gcgggcgttc gcggcagcct cacggggatt cggctggctc
      961 gagggcgttg cgccgctggc ggcgcccgag catccagcgc ccctcccgga cggccccctc
     1021 gccgtgcggt tcgagagcgt ccacttcagc tacggaacag gtgtggaagt gctccggggc
     1081 atcgacctcg atgcgcgccc cggcgagatc ctggcgatct gcgggccgac cggcgtcggc
     1141 aagacgtcgc tgctcaatct catcccccgc ttctacgacc cagacgccgg gagggtgctg
     1201 ctgggcggcg tagcgaccga cgagacaagc ctcgacgagc tccggagcgc ggtcgccctg
     1261 gtcacgcaga agccagtgct cttctcggtc accctgcgcg acaacctcgt cgccgcgcgt
     1321 ccggatgcgc cgtgggaagt cgtgctggcg gcgagtgagg cggccggtgt cgaagcgttc
     1381 gtctccgacc ttccgcacgg ctacgagacc atgatcggcg agcggggggt caacctctcc
     1441 ggcggacagc gacaacgggt cgcgctcgct cgggcgctga tcgcagatgc acgcgtgctc
     1501 gtactcgacg atccgatgtc cgccgtcgac acgcagaccg agcggttgct ggtggaaaac
     1561 cttcgccccg cggtggccgg gcgaacggtg ctgatcgccg gccagcggct ctcgaccatc
     1621 ctcgtcgccg accgtgcggt cgtgctcgaa gacggtcgaa tcacagaaca ggggactccg
     1681 ggcgagctgc tcaaccgggg cggagcgttc gcggcgctgt tcggagacga gacagttgcg
     1741 gcgtaggcga cgcacgggat tgacacgcct gtggcgctat tccgccgggc gtcggcggca
     1801 catcgccctc gtgacctcgc tcgccggcat ctcggcggca gcgccggtcg cgggctggct
     1861 tctcgtcgga gacgcgattg acaacggcat cagggccggc gacgagagcc gtctcacgat
     1921 ggacgtcatc gcctacatcg gcgtcaatgc tgtggcctgg ctacttggct cgacgacgtg
     1981 gttgatgctc gccggaatcg gccaagacat cgtgctcgcg gtgcgacgag acctcttcga
     2041 gcatctcacg tcgctctcgc ttcgctactt ctcggagcag aaggcgggct ggatcatcgc
     2101 acggctgacg agcgacgtcg acgcgctgtc cgacgtcctc acgcaggggc tgaccacact
     2161 cgtcgtcaac acgctcacgc tggtcgcggc tgtcgccggg ctcttcctgc tcgactggcg
     2221 cctcgggctt gtcgccctgc tcgtgctccc tcccggcacg ctgatcacac gctggttcca
     2281 gcgacgctca cacgcggctt tcgcggaggt gaggacgcgg atagcggccg tgaccgccca
     2341 gctcgcggaa tcggtgtccg gaatggcgat cgtgcaggcc ttcaaccgcg aacgggcgtt
     2401 ccagagcgag ttcgagcagc tgaacgacga gaaccgaaag gccaactacg tcgcccagaa
     2461 ggtctcgtcg gtcttcttcc ccgccgtgca gctcctcggc gtcgtcgcga ccgttgcggt
     2521 actgctggcc ggagcgctcc tcatcgacgc cggctcgctg gagatcggga cgctggtcgc
     2581 tgcgatcgga ctgctgcagc tggtcttcca gccgctccag gaactgtccg agctctacgg
     2641 ccaggttcag tcggccaacg ccgcgatgga caagatcagc tcggtcctgg acgcagagca
     2701 agacatcaaa gaccgccccg gagccaagcc gatcgggcgg gtcgacggtc atctgcaact
     2761 cgatgacgtc gtgttcgcgt acggggagga gccggtcatc cgcggtatct cgatcgacgt
     2821 gcggacgggc ggctgcatcg cgctcgtagg cgagtccggc ggcggcaagt ccacgacggc
     2881 gaggctgatc gcgcgcttct acgatccgct cgagggcacg attcgcgtcg acggcaagga
     2941 tctccgcgac gtcaagctcc gcagctaccg ccgtcagctg ggcgttgtac tgcaggaccc
     3001 gttcctgttc agcggcacga tcgccgacaa catccgcttt gcccggcccg atgccgctga
     3061 cgcagaggtg cgggctgcag ccgaggcggt cggggtcgac cgggtcgcgg cacgcttcga
     3121 ggacggcctc gatcatgtcg tccgtgaggg cggagcgggg ctctcggccg gcgagcggca
     3181 gttgatctcg atcgcgcggg cgctccttgc cgacccgcga atactcattc tcgacgaagc
     3241 cacgtcgaac atcgaccggc cgaccgagat tctgatcgag cgcgccttcg accgcctcct
     3301 cgcggcgcga acctcgatca tcatcgcgca ccggctgaca acggtgagac gcgccgacga
     3361 gatcctcgtc gtcgagcacg gccggatcgt gcaacggggg atcgagtccg agctgctggc
     3421 gcaagaaggc ccctttcggc agctggcgca cacactcgaa gcagcgtcgc tgaaggccgc
     3481 ggtgtgagcg gcgctcagcc gtccagcagc caggcttcgt cgcggatgat cggggttgcc
     3541 gcacccttcg cgtcgagccc gtcgacctcc acgtccggtc cgccgatcat gaaatcggtg
     3601 tgcactcccg acacgttgat cccgagctcc acgagctcgt ccgggccgag gccggccgcg
     3661 tccgcgtgcg tgaacggaag acccgccccg taggcaatgt gacaggtcgc gttctcgtcg
     3721 aagagcgtgt cgtggaagac gagtccggtt tcccgtacac gcgacgtgcc gtccacgagc
     3781 gcgacctcac cgaggtaggg cgcgttggca tccgtcgcaa gctgcgcctt gatggtctcg
     3841 actccggtcg tcgcctcgac gtcgacgatc ttgcctcctt cgaaccgcat tgccaggttc
     3901 tcggcgagca cgcccgtgcc cggaacggcg aggggatagg tcgagcggac gctgccgtct
     3961 gcgcggcggc ggtctgggct tgtgaagacc tcttccgtgg ggatgttggg caggtgctgc
     4021 aggccggact gggtcgtgaa gagcgcgctc agccagctcg cgccgggaag caagccgatc
     4081 gtgagatcgg tgccgggccc ccggaaacgg atcgagtcga atcgcctttc ggcaagcgtg
     4141 ctcgaccgtg ctccgagcac ggccgcccgc tcggtccagg cggcgacggg atcggcttcg
     4201 tcgaggcgca tcgccgtcgc gaccgctccc cacaggcgct cgacgtcggg ctcgccgaag
     4261 acgctcgtcg cccaaccctc gttcggcgcg gggacgatga cccagttcag cagccgctcg
     4321 gtgacgagcg gcaggtatgc ggcacgcagc tctatcggct cggacttcgc gacccgctcc
     4381 gggtcgaacc gcgacagcag gtgaaggtcc ggattgccgg tgagcgagat gagcgccggc
     4441 cgcgtctcac gccagctctt cacccaatcg agcaggtacg ggggcgtcca gccgagcgac
     4501 tcctcgggcc cgaactcgac gagcgctgcc cggaagtgcg cgtcgccgta gaggctcatg
     4561 acgtgagacg ccccgacgcg ataggcctgg cgcgcaacgg ctcgggcgat ctccgcgtgc
     4621 tcgacctgcg ccatcacgac gacgtcctgt cctcgctgaa cgttcgcccc gaggcgtacc
     4681 accagctcgg cgtagcgatc gacgcgctcc tccggcgtca ccgcaggacg accccgttca
     4741 catgtgagag aaagctcagt cgccccagtc gaagtcgcgt gagcgacccc aaagggcctc
     4801 gtcgagcgcc gcgccgccct cttcagcggc ctcctccccg ggccaggaga gggggcctgc
     4861 ggccaccgcg gcaggagctt ctgccccgtc catagtctcc tcgatgcgtg cttgttcctc
     4921 ggacttgaac agcgggttgt tctcgaagag atcctcgccc gagtaggtgt cgatgggcag
     4981 tcgttcgtcg agcgccgagt tctttgcctt caggtcgaga tggtcttgga tggcgcgggc
     5041 gaaggcggac tctgtctcga ctgtcatacg tgctcctcgg gttgtcgtgc aggcggtttg
     5101 atgcgcgagg attctaccgc agcgtgaatt ccctgcaagc agatggttcc ctcccgccgg
     5161 gtagcattga gccgatgcca ccgatccatc gaattccgcg gcatcccgtc acactcgccc
     5221 tcttcgcgta ccgaacgtgg taccggctac cgccagccca gcgccgacag gtgctagaag
     5281 cagcaaagac acacgggccg aagatcgcgt cgatcgcggc ggcgactgcc cgccgcacgg
     5341 tcgacaaggc tcgcaaaccg gcaccgtagg gggcttcagt gccgtgcgag cgcttctcgc
     5401 acgatccggt cctgctcgcc cagatgggcc gtcgggtagc cctcgctcgg actggcccgc
     5461 ca
//
LOCUS       DBNGNK010000029         3835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_219113,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000029 DBNGNK010000000
VERSION     DBNGNK010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_219113"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="AC150V_00755"
     CDS             <1..1010
                     /locus_tag="AC150V_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3761483.1"
                     /translation="LVGGSGVWWDDAAPQLEQLAERVGIPVFLNGSGRGSLAPDHPYL
                     FQHARSTALAEADVVLIVGATLDFRLRYGRFDAPTLIHVHSDPRELGRNRTPELGIAS
                     DSALLLAALAEALPAAAPRADWLDLLRASEDAWWEKHRGEIESDAAPLHHYRLGAELA
                     KVLEPGTVVIGDGGDVVAAVSRVLRVGRPGHWLDPGPFGCLGVGPGYALGVGAARFGD
                     AIVIVMGDGAFGLNGLDYDSLVRFGIPAVLVIGNDGAWGEIRVPQVGIYGEEGEIATR
                     LAPSRYERVCDVFGGHAEHVERPAGLAPALERALSAGEPAIVNVMLDPDAMAGHAYRG
                     M"
     gene            complement(1033..1272)
                     /locus_tag="AC150V_00760"
     CDS             complement(1033..1272)
                     /locus_tag="AC150V_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR3761484.1"
                     /translation="MFVRVLILAAAVLLIWAVVARDSGAGGPERLYRVQAGDTLWSIA
                     VANFGGDPREGVWIMRERNGLSGTTIRPGQRLVLP"
     gene            complement(1318..2778)
                     /locus_tag="AC150V_00765"
     CDS             complement(1318..2778)
                     /locus_tag="AC150V_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761485.1"
                     /translation="MGRLVAAALAAAALLAAPVGRAADIGANDDTGKFAGEGATAYYE
                     HMAELGLEQSILTVRWQPTRPDVIPDREFLDNAVSAAALSGIQIVFAVYPYPPRELQS
                     GLASPSGFADFVEQVAERYPAVEQFVIGNEPNQTAFWRPQLTRKGAVRSAATFGRYLA
                     AGYDALKTVDPGITVVGVGLSPRGNDNPRARSNRSTSPVRFISALGAWYRKSGRTEPL
                     MDGFSYHPYPRTAEHSLMRSYPWPHAGFRDLSRIKQALYDAFAGTAQPTTLDGLQLYL
                     DEVGWQVDTTLQEGYTGRENVAVTTERRQAAVYGNLVRRAACDPDIAQVNFFGFHDDA
                     ARTGFQAALHRLDGSPRASAGSVSRAIRATSSGCKGKLVRWRPAGKVFGASAARWRPQ
                     RSGALKLLIGAKEGARAVVCLVRAPAQGVRRVASHAALRRQAIAPCWRGRLTPRMRRE
                     VKLAPPVGAGPFTVVARLFAEANPKRVSLLLPRHSR"
     gene            complement(2872..>3835)
                     /locus_tag="AC150V_00770"
     CDS             complement(2872..>3835)
                     /locus_tag="AC150V_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761486.1"
                     /translation="PGITVVGLGVSSRGNDRPRAKNNVSTSPVRFVAALGVWYRKSKR
                     KRPLMDAFSFHPYPNSNREPLAKGFQWPNAGVVNLGRVKLALFDAFNGTAQETPADGL
                     PLLLNEVGWQVDTSRNPAYVGEERVVVTTEPHQANVYRQIVRRAACDTNVSGLSFFAF
                     YDQREREGMQSGLFRVDGSPRLSAGKVSNAISRAGKGCTKRERAQLAKGWKAPKGVVG
                     AKAFFTKWKKRCYGVQGSKHLCLTAQENARYRAAAFPAGTPRKEMMRALRRGAEVKGK
                     LKAYSRPALTLTPPAAGGPWVVAVKVIAAFNKKRAALIVGPVIR"
BASE COUNT          562 a         1327 c         1347 g          599 t
ORIGIN      
        1 cgctggtcgg cggctccgga gtctggtggg acgatgccgc gccgcagctc gagcaactgg
       61 ccgagcgcgt ggggattcct gtgttcctca acggatccgg gcgcggatcg ctcgcccctg
      121 accatcccta tctcttccag catgcccgct ctactgcgct cgcggaggcc gacgtcgtcc
      181 tgatcgtcgg cgcgacgctc gacttccgtc tgcgctacgg ccgcttcgac gcgccgacgc
      241 tcatccacgt ccattccgat ccacgggaac tgggccgcaa ccgcacgccg gagctcggca
      301 tcgcctccga ctcggctctc ctcctcgccg ccctcgccga ggcgcttccg gcggcggcgc
      361 cccgcgcgga ctggctagac ctgctgcgag cgtccgagga cgcctggtgg gagaagcatc
      421 gcggcgagat cgagtcggac gcagcccccc tccaccacta ccgactcggc gccgagctgg
      481 cgaaggtgct cgagcccgga accgtggtga tcggcgacgg aggcgacgtg gtggccgccg
      541 tctcccgtgt cctgcgagtc gggcgtcccg gccactggct cgaccccggc ccgttcggct
      601 gtctcggagt cgggccgggc tacgcgctcg gcgtcggcgc ggcccgcttc ggcgacgcga
      661 tcgtgatcgt gatgggcgac ggggcgttcg ggctgaacgg tctcgactac gactcactgg
      721 tgcgcttcgg catccctgcc gtgctcgtga tcggaaacga cggcgcatgg ggcgagatcc
      781 gtgtcccgca ggtcgggatc tacggcgagg agggcgagat cgcgacgcgt ctggcgccga
      841 gccggtatga gcgggtctgc gacgtcttcg gaggtcacgc agagcatgtc gagcggcccg
      901 cggggctggc ccccgcgctc gagcgggccc ttagcgcggg cgagccggcc atcgtgaacg
      961 tgatgctcga cccggacgcg atggccggac acgcctaccg cggcatgtag cagcgcgacg
     1021 gccggtcggc cgctagggaa ggacgagacg ctgtccgggc ctgatcgtcg tgcccgagag
     1081 accgttgcgc tcgcgcatga tccagactcc ctcgcgcgga tcgccgccga agttcgcgac
     1141 ggcgatcgac cagagcgtgt caccggcctg cacgcgatac agccgttccg ggccgcccgc
     1201 acccgagtcg cgcgctacga ccgcccagat gaggaggacg gcggcggcaa ggatgagaac
     1261 acgtacgaac atgtgttcgc cagtatggac gaacacgtgt tcggtgtcaa ctgcggctca
     1321 gcggctgtgc ctgggaagga ggagcgagac gcgcttcggg ttggcctcgg cgaacaatcg
     1381 ggccacgacc gtgaacggcc ccgccccgac aggcggggcg agcttcacct cgcggcgcat
     1441 gcgcggcgtc agtcggccgc gccagcacgg agcgatggcc tggcgccgca gggccgcgtg
     1501 cgaggcgacg cgacgaacgc cctgcgcggg ggcgcggacg aggcacacga ctgcccgtgc
     1561 gccctccttc gcacctatca ggagcttgag cgcacccgag cgttgcggcc gccagcgtgc
     1621 ggcggaagct ccgaaaacct tgccggcggg ccgccagcgc acgagcttcc ccttgcagcc
     1681 gctcgacgtg gctcggattg ccctgctgac gctgcccgcc gacgcacgcg gcgagccgtc
     1741 caggcgatgc agtgccgcct ggaaccccgt tcgcgccgcg tcgtcatgga agccgaagaa
     1801 gttgacctgt gcgatgtcgg ggtcgcaggc ggcgcgtcgc acgaggttcc cgtacacggc
     1861 tgcctgacgt cgctcggtgg tgaccgcgac gttctcccgg cccgtatagc cctcctggag
     1921 ggtggtgtcg acctgccaac ccacctcgtc gagatagagc tggaggccgt cgagggtcgt
     1981 gggctgcgcc gtgccggcga aggcgtcata gagcgcctgc ttgatgcggc tgaggtcgcg
     2041 gaagcccgcg tgcggccatg ggtagctgcg catcagactg tgctcggccg tgcgagggta
     2101 tgggtgatag ctgaagccgt ccatcagcgg ctccgtccgc cccgacttcc ggtaccacgc
     2161 gcccagggcg ctgatgaagc gcaccggcga cgtcgagcgg ttgctacgcg cacgcgggtt
     2221 gtcgttgcct cgcggcgaga gaccgactcc tacgacggtg atgccgggat caaccgtctt
     2281 cagggcgtcg tagcccgcgg ccaggtagcg tccgaacgtc gccgccgagc gcacggcgcc
     2341 ctttcgcgtc agctgcggcc gccagaaggc ggtctgattg ggctcgttgc cgatcacgaa
     2401 ctgctccacc gccggatagc gctcggcgac ctgctccacg aagtctgcga agccggacgg
     2461 gctcgccagg ccggactgca gctcacgtgg cggatacggg tagacggcga acacgatctg
     2521 gatgccgctc agcgccgccg ccgacacggc gttgtcgagg aactcgcgat cgggaatgac
     2581 gtcgggacgc gtcggctgcc agcggacggt caggatcgat tgctcgagac cgagctcggc
     2641 catgtgctcg tagtaggccg tcgcgccctc acccgcgaac tttccggtgt cgtcgttggc
     2701 gccgatatcg gcggcacgtc cgaccggcgc cgcaagcagt gcggccgccg cgagcgcagc
     2761 tgcaacgagt cgtcccatcc ctgttccggt tctcccctcg ctcgctccgc agcccgaaag
     2821 ggcctcgaag cggcggcagt ctacgagatc gatcggaaga cgtctagccg actaccggat
     2881 gaccgggccg acgatcagcg ccgcgcgctt cttgttgaag gccgcgatca ccttgacggc
     2941 gacgacccag gggccacccg cggccggtgg tgtgagggtc aaggccggcc gggagtacgc
     3001 cttcagcttg cccttgacct ctgcacctcg tcgaagcgcc ctcatcatct ccttgcgggg
     3061 tgtgcccgcc gggaaggcag cggcccgata gcgcgcgttc tcctgcgccg tcaggcagag
     3121 gtgcttcgag ccctgcacgc cgtagcagcg cttcttccac ttcgtgaaga acgccttggc
     3181 gccgacgacg cccttcggtg ccttccagcc tttcgcgagc tgagcgcgct cgcgcttggt
     3241 gcagcccttg ccggcgcgtg agattgcgtt cgagaccttg cctgccgaca aacgcgggct
     3301 gccgtcgacg cggaacaggc ccgactgcat cccctcgcgc tcgcgctggt cgtagaaggc
     3361 gaagaacgag agcccgctga cgttggtgtc gcacgcggct ctgcggacga tctgccggta
     3421 gacgttcgcc tggtgaggct cggtcgtcac gacaacgcgt tcctcgccga cgtacgcggg
     3481 attgcggctc gtgtcgacct gccagcccac ctcgttgagc aggagcggaa gcccgtccgc
     3541 cggcgtctcc tgagccgtgc cgttgaaggc gtcgaagagc gccagcttca cacgcccgag
     3601 gttgacgacc cccgcgttgg gccactggaa gcccttcgcg agaggctcgc ggttcgagtt
     3661 cgggtaggga tggaagctga aggcgtccat gagcggccgc ttccgcttgc tcttccgata
     3721 ccagacccca agcgcggcga cgaaccgcac gggcgaggtc gagacgttgt tctttgcgcg
     3781 cggccggtcg ttgccgcggg aggagacacc cagaccgacg accgtgatcc cggga
//
LOCUS       DBNGNK010000030         5617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1007613,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000030 DBNGNK010000000
VERSION     DBNGNK010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1007613"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="AC150V_00775"
     CDS             complement(<1..222)
                     /locus_tag="AC150V_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013405924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR3761487.1"
                     /translation="MLVKKIQQRLAKEESGFTLIELLIVLVIIGILLAIAVPSYLGFK
                     DRAENRAAASNVRAALPAAEAFYADNGTYT"
     gene            complement(364..780)
                     /locus_tag="AC150V_00780"
     CDS             complement(364..780)
                     /locus_tag="AC150V_00780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR3761488.1"
                     /translation="MIGSTLMMGATQPERQLKDERGFTLVELLVVLVILAALILLGYT
                     AMQRYHDRAANAAAKASLRQAIPAAEAYFADNKTYTGLNNAGLAAYDSAVSATLAVVS
                     ADATSYCLTETVRSRTWSSAGPGVATSDYVANATCL"
     gene            897..2093
                     /locus_tag="AC150V_00785"
     CDS             897..2093
                     /locus_tag="AC150V_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012866771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3761489.1"
                     /translation="MPIRLAVELNEHPDLSAPLSIDELLERTVELSASDLHITAGSPP
                     AVRVNGHLQLLEDYAELDSDSVRELVYRITTTEQQKHLEVARQLDFAYGLRGLSRFRV
                     NAFYQRESLAAAFRTIPTQIMALEELGLPPALHELTTKPRGLVLLTGPTGSGKSTTLA
                     SLIDEINRTRPDHIVTIEDPIEFLHQHKRAIVNQREIGQDATGFADALRAALRQDPDV
                     ILLGEMRDLETISTALTAAETGHLVFATLHTQTAPSTIDRIIDVFPAAQQDQVRQQLA
                     NALQGIVTQTLLPKADGQGRVCALEILFLDDAIRNLIRQGKIEQVYSYMQTGTRRGMQ
                     TMEQSLLDHVQSGRITASEALGRSSRPEALVSALERAGVPIPAMSTDGAPIASAMGGG
                     LRVAGS"
     gene            2103..4676
                     /gene="pilM"
                     /locus_tag="AC150V_00790"
     CDS             2103..4676
                     /gene="pilM"
                     /locus_tag="AC150V_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HIR3761490.1"
                     /translation="MTPWNPTTPFSGFTPDTGVAGPSEPRAMDDRAASHPLPQPAARK
                     SLGFVDASETTASDGASRGEIAYRTELSFPIRAWESPRAEGDVPFYKREITFRRKSKP
                     EQAPEPESLDADDMDLASTDEPKQPFGFGAPDRDEDSPVADESDLEPEADETGMDVAA
                     DESNFDATADEPELEIVADESDSDATADEPEFEIVSDASELADDLAVEPVEAMDMLAE
                     DDLTHATPLAFEPVSEEMDADAPVEHTESGTSMDDEQSVIDEASVPVEPVVVAPEPPA
                     KPAKASRNGRGRHRGKGRKVVGLKIGATGISAAVVANDGGRHELQDLVRVPIEQGVVV
                     DGEVRDAVALERALKSLFSDHKLPRDVRIGLASNRIGVRTFEIAGIDDEERFNNAVRF
                     KAHEVLPIAGTESVLEYRVIGSRHTETGEEMRRVLLVVAPSDQISPYVEVCRTAGVRL
                     SGIDLEALGLLRAFVHPDLARSEAGQDTATVIVGMGHESSTLLVAGGGAAEFTRVFDW
                     GGGVLQSAVAEELDVTPLEASTILRQISLTSGVEFGTELEADVRARAVEAVRLRLTPF
                     ARELVASLQFYQTQPESLGIGEIVITGGTSRLDGLADFLNQMIGVSVRVGDPLARVEV
                     AQNALDNVDEADLPSFSVPIGLAIEDEQMRSVNLVPAEQRPAGVGKPRLAVVALPAVA
                     ALAAVAVGGAFFLSQGTVSDRESDLQAAEAEFASLPEPGGLSIVAPGVEQETASRATS
                     VAAVLGTRLNWDHMLNDLSRVLPADVWLTTMSAQLPVPTPEDAAQATSNPAPTSGQTG
                     VEIQGYTYQQADVATLLSRLQTLPTLSDVELRSSEVAEVADKPVVQFNIAANLRSGGT
                     G"
     gene            4673..5335
                     /gene="pilO"
                     /locus_tag="AC150V_00795"
     CDS             4673..5335
                     /gene="pilO"
                     /locus_tag="AC150V_00795"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HIR3761491.1"
                     /translation="MKALLSNPKAVAGISVAVAAVVLAGGWFLLVNPQREEAAKLDGE
                     IAAVDKRIAQRKAELSSPKREVRIRQSDLYRLRRAMPSVVDMPGVMLDLDRLAKRAEV
                     KFDAITPQGEVALNGFAMRPIDVVLSGRFTNVSRFLRELRTLVRVRKGKLDARGRVFA
                     VDSVQFIESKDGFPDVEATLRVNAFVHGSGALPPGVQNEAAPNEETSGPNTSVSAVGA
                     NP"
     gene            5335..>5617
                     /locus_tag="AC150V_00800"
     CDS             5335..>5617
                     /locus_tag="AC150V_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761492.1"
                     /translation="MTTTTNTTQIEQQRKKKQIIILAVIGGLLVLLMAFQLPKLLGGN
                     EAETAVADETAAETSADAAAGGTPPPSTTTDATTGTPVATLPIPAGKPTS"
BASE COUNT          938 a         1951 c         1863 g          865 t
ORIGIN      
        1 ggtgtaggtg ccgttgtcgg cgtagaaggc ctcggcggcg ggaagcgcgg cgcggacgtt
       61 ggaggccgcg gcgcggttct cggcgcgatc cttgaagccg aggtacgagg gaactgcgat
      121 cgccagcagg atgccgatga tcacgaggac gatgagcagc tcgatgagcg tgaaaccgct
      181 ctcttctttc gcgagccgct gctggatctt cttgacgagc attcgtactc cttccgggaa
      241 gggtggaacc gtcgatccca tatcgggcga gatcggcttt ttgctctacc ccccaacggg
      301 tgattagagg gtgatcgttc gagggatccg gcacgggcgg gccgcagccg cccgcgcgga
      361 tccctacaag caggtcgcgt tcgcgacgta gtcagaggtg gcgactcccg gaccggcact
      421 gctccaggtc cggctcctga cggtctccgt gagacagtag gaagtcgcgt ccgcgctgac
      481 gacggccagc gtggccgaga ccgccgagtc gtatgcagcg aggccggcgt tgttcaggcc
      541 cgtataggtc ttgttgtccg cgaagtaggc ctcggccgcc ggaatcgcct gtcgcaggct
      601 cgctttggcg gccgcgttgg ccgcgcggtc gtggtagcgc tgcatcgccg tgtagccgag
      661 caggatgagc gccgccaaga tcaccaggac gacgagcagc tccacgaggg tgaagccgcg
      721 ctcgtccttc agttgcctct ctggctgcgt agcccccatc atcagcgtcg atccgatcat
      781 cggcggattc ccgtgtgccg ggtacccccg tgcgggtgat cgtcggggaa ccgatcaccc
      841 acaatggggg atatgccggc gggtcttccc ccggccactc aagtggatgg cccggcgtgc
      901 cgatacgcct ggccgtggag ctcaacgagc accccgatct ctccgcgccg ctctcgatcg
      961 acgagttgct tgagcggact gtcgagctga gcgcgtccga cctgcacatc accgcgggct
     1021 cgcccccggc cgtgcgggtg aacggacacc tgcagctcct cgaggactac gccgagctcg
     1081 actcggactc cgtccgtgag ctcgtgtatc ggattaccac caccgagcag cagaagcacc
     1141 tcgaagtcgc ccggcagctc gacttcgcct acggcctgcg tgggctgtcg cgcttccgtg
     1201 tcaatgcctt ctatcagcgc gagagcctcg ccgcagcctt ccggacgatt ccgacccaga
     1261 tcatggcgct cgaggagctc ggccttcctc ccgcactcca cgagctgacg acgaagccgc
     1321 gcgggctcgt gctcctgacc ggcccgaccg ggtcgggcaa gtctacgacg ctggcttcgc
     1381 tgatcgacga gatcaaccga acccgccctg atcacatcgt cacgatcgag gacccgatcg
     1441 agttcctgca ccagcacaag cgggcgatcg tcaaccagcg cgagatcggc caggacgcga
     1501 ccggcttcgc cgacgctctc cgcgccgcgc tccggcagga ccccgacgtg atcctcctcg
     1561 gcgagatgcg cgacctggag acgatctcga cggcactcac cgctgcggag acggggcatc
     1621 tcgtcttcgc gaccctgcac acgcagaccg cgccgagcac gatcgaccgc atcatcgacg
     1681 tctttcccgc agcccagcag gatcaggtgc ggcagcagct cgccaacgcc cttcagggca
     1741 tcgtcaccca gaccctcctc ccgaaggccg acggtcaggg gcgggtgtgc gcgctggaga
     1801 tcctcttcct cgacgacgcg atcaggaacc tgatccgcca ggggaagatc gagcaggtct
     1861 actcgtacat gcagaccggc acgcgccgag gaatgcagac gatggagcag tcgctgctcg
     1921 accacgttca gagcggtcgc atcaccgcct ccgaggcgct cggccgctcg agccgtcccg
     1981 aagcgctggt ctcggcgctc gagcgggccg gtgtccctat ccctgcaatg tcgacagatg
     2041 gcgctccgat tgcctccgcc atgggcggcg ggctgcgggt cgccgggagc tgagagccgc
     2101 ccatgacacc ctggaacccc accacaccgt tctcgggatt cactcccgat accggcgtcg
     2161 ccggcccgag cgagccgcgg gcgatggacg atcgggccgc ctcgcacccg ttgccccagc
     2221 cggctgcccg caagtcgctc ggcttcgtcg acgcgagcga gacgactgcg agcgacggag
     2281 cgtcgcgcgg cgagatcgcc taccgcactg agctttcgtt ccccattcga gcctgggaaa
     2341 gcccccgggc cgagggagac gtccccttct acaagcgtga gatcaccttc cgccgcaaga
     2401 gcaagccgga gcaggcgcca gagcccgagt cgctcgacgc agacgacatg gacctggcgt
     2461 ctacggacga gccgaagcag ccgttcggct tcggcgcgcc cgaccgggac gaagacagcc
     2521 cggtcgccga cgagtccgac ctcgagccgg aggcagacga gaccgggatg gacgtcgcgg
     2581 cggacgagag caacttcgac gctaccgccg acgagcccga gctcgagatc gtggcggatg
     2641 agagcgattc cgatgcgacc gccgacgagc ccgagttcga gatcgtgtcc gacgcgtcgg
     2701 agctggcgga cgatctcgcc gtcgagccgg tcgaggcaat ggacatgctt gcggaggatg
     2761 acctcaccca tgcgacgcct ttggcgttcg agccggtgtc cgaggaaatg gacgccgacg
     2821 caccggtcga gcacacggag tccggcacgt cgatggacga cgagcagagc gtgatcgacg
     2881 aggcatccgt ccccgtcgag ccggtcgttg tggcacccga gcctccggcc aagccggcaa
     2941 aggcgagccg gaacggtcgc ggccgccacc gcggcaaggg ccgtaaggtg gtcgggctca
     3001 agatcggggc cacgggcatc tcggcagcgg tcgtagcgaa cgacggcggt cgccacgagc
     3061 tgcaagatct cgtccgagtc ccgatcgagc agggtgtcgt ggtcgacgga gaggttcgcg
     3121 atgccgtcgc cctcgagcgc gccctcaagt cactcttctc cgaccacaag cttccccgag
     3181 acgtcaggat cggcctcgcg tcgaacagga tcggcgtccg caccttcgag atcgcgggca
     3241 tcgacgacga ggagcgcttc aacaacgccg ttcggttcaa ggcgcacgag gtgctgccca
     3301 tcgcaggcac cgagtccgtc ctcgagtacc gagtgatcgg ctcccgtcac accgagacgg
     3361 gcgaggagat gcggcgcgtc ctcctggtcg tcgccccctc tgaccagatc tcgccgtacg
     3421 tcgaggtgtg tcgcacggcc ggcgtccggc tcagcggcat cgatctcgag gcactcggcc
     3481 tgctccgcgc cttcgtccat ccggatctcg cgcgcagcga ggccggccag gacaccgcga
     3541 cggtcatcgt cggcatgggc cacgagtcct cgacgctgct cgtcgcaggc gggggtgccg
     3601 ccgagttcac ccgcgtcttc gactggggtg gcggtgtgct ccagagcgcc gtcgccgagg
     3661 agctcgacgt gacaccgctc gaggcctcca cgattctccg ccagatctcg ttgacgagcg
     3721 gtgtcgagtt cggaaccgag ctcgaagccg acgttcgtgc gcgtgcagtc gaggcagttc
     3781 gcctccgtct gaccccgttc gcccgtgagc tcgtcgcctc gctccagttc taccagacgc
     3841 agcccgaatc cctgggcatc ggcgagatcg tgatcacggg aggcacctcg cgtctcgacg
     3901 gcctcgccga cttcctcaat cagatgatcg gcgtctccgt acgggtcggc gatccgctcg
     3961 cgcgcgtcga ggtcgctcag aacgctctcg acaacgtcga cgaagccgac ctcccctctt
     4021 tctcggtgcc gatcgggctc gcgatcgagg acgagcagat gcgctcggtc aacctcgtac
     4081 cggcggagca gcgccccgcc ggcgtcggca agcctcgcct tgccgtcgtc gcgcttccgg
     4141 cagttgcagc gcttgcagcc gtcgccgtcg gcggagcctt cttcctcagt caaggcacgg
     4201 tctcggatcg cgagagcgac ctgcaggcgg ccgaggcaga gttcgcttcg ctgccggagc
     4261 ccggcgggct gtcgatcgtc gctcccggcg tcgagcagga aacggcctcc cgggccacgt
     4321 ccgtcgcggc tgtgctgggc acgcggctga actgggatca catgttgaac gacctctctc
     4381 gcgtgctccc cgccgacgtc tggctgacca cgatgagcgc tcagctcccg gtgcccacac
     4441 ctgaagacgc cgcccaggcg accagcaatc cggccccgac ctccggccag accggcgtcg
     4501 agatccaggg ctacacctac cagcaggccg acgtggcgac gctgctctcg cggcttcaga
     4561 ccctgccgac gctgagtgac gtcgagcttc gctcgagcga agtggccgag gtcgcagaca
     4621 agccggtcgt gcagttcaac atcgccgcca acttgcgctc aggaggcacc ggatgaaggc
     4681 tcttctctcc aaccccaagg ccgtagccgg aatctcggtc gcagtcgccg cggtcgtcct
     4741 cgcgggcggc tggttcctcc tcgtcaatcc gcagcgggag gaggcggcca agctcgacgg
     4801 cgagatcgcc gcggtcgaca agcggatcgc tcagcgcaag gccgagctct cgagccccaa
     4861 gcgcgaggtt cgcatccggc agagcgatct gtacaggttg cggcgcgcga tgccgtccgt
     4921 ggtagacatg cccggcgtca tgctcgatct cgaccggctc gccaagcggg ccgaggtcaa
     4981 gttcgatgcg attacgccac agggcgaggt ggcgctgaac gggttcgcga tgaggccgat
     5041 cgacgtcgtc ctctcgggcc ggttcacgaa tgtgtcccgc ttcctgcgcg agctgcgaac
     5101 gctggtgcgg gtgaggaaag gcaagctcga tgcgcgcgga cgcgtgttcg ccgtggacag
     5161 cgtgcagttc atcgagtcca aggacgggtt cccggacgtc gaggcaacgc ttcgcgtcaa
     5221 cgccttcgtg cacggcagcg gcgcgctgcc gcccggcgtc cagaacgagg ccgctccgaa
     5281 cgaggagacc tcgggcccga acacgtccgt gagcgccgta ggagcgaacc cctgatgacg
     5341 actacgacga atacgaccca gatcgagcag cagaggaaga agaagcagat catcatcctc
     5401 gccgtcatag gcggcctcct cgtgctgctg atggccttcc aactgcccaa gctgctcggc
     5461 ggcaacgaag ccgagacggc ggtcgcggac gagacggcgg ccgagacgag cgccgacgcc
     5521 gctgccgggg gcacgccccc cccctcgacg acgaccgacg ctacgaccgg cacgccggtg
     5581 gcgacgctcc cgatcccagc cgggaagccg accagta
//
LOCUS       DBNGNK010000031        13426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1938776,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000031 DBNGNK010000000
VERSION     DBNGNK010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 13426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13426
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1938776"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="AC150V_00805"
     CDS             complement(<1..656)
                     /locus_tag="AC150V_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761493.1"
                     /translation="MRLATITIARRYCGPSDSGNGGYTCGLLAAFVEAPEVEVTLRSR
                     PPLEQELHVDSVDGGALLAADGELIAEAVAGSLGVAAPAPVSPDEAIDAASRYIGFHE
                     HAFPECFVCGPSRDVGDGLRIFPGPIAGRSDIVAAPWTPDEVNEPVVWAAIDCPGAFA
                     CGMSDRGEVVLGRMTARIARLPLQGEHCIAVGWPIGSDGRKLFAGTALFGGAGEVLAL
                     S"
     gene            716..2428
                     /locus_tag="AC150V_00810"
     CDS             716..2428
                     /locus_tag="AC150V_00810"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR3761494.1"
                     /translation="MRLALAQLNVVVGDLDGNRDRIVDALAEARGRGADVAIFPELAV
                     TGYPPEDLLLRPGFVRAARRALDQIAAASTGMTALVGTPWLESDLFNACAVCADGEVR
                     GVYRKHFLPNYGVFDEHRYFAEGRDLLLLRLGDALLGPTVCEDVWQPGPPATDLALAG
                     AHLLVNISASPFHVGKAEDREEMLVTRARDNTCYLAFCNLVGGQDELVFDGHSVVLDD
                     EGEVIARAKGFEEELLVVDIDPTEAVARRLRDVRRRELGRSRGGAPAAPVIELGEPAR
                     SERPVTAATAAFAPELEQMRRALVLGLRDYVGKNGFGDVVVGVSGGIDSAVTAAMCVD
                     ALGAERVHCVSMPSRYSSTETRTDARRLADNLGCDFHELSIEAAVESFSTVLAASIEG
                     GPSGLAAENLQARIRGTMLMALSNQFGWLVVSTGNKSELAVGYSTLYGDMVGGFALLK
                     DVFKTDVSRLARYVNERAGEDRIPVTTIERAPSAELRADQLDEDSLPAYEALDQVLEA
                     YVEEDRSREELLAAFDPDVVERALDLVDRAEYKRRQAPPGVKLRPKAFGRDWRPPITN
                     LWRG"
     gene            complement(2406..3005)
                     /locus_tag="AC150V_00815"
     CDS             complement(2406..3005)
                     /locus_tag="AC150V_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HIR3761495.1"
                     /translation="MNSPEGSASEGVDATRRTHLANERTYLAWWRTGLGAVAGGFALG
                     ALGIEFSAGPTGRLRSPAPSTSPAASASCSTAGTGSGGWAWRFGGASMRGPHRHCSAG
                     SPSRAHCPGSPRSSCCSRSERTAFVSVPPPAHSFSTRTTACSSSAFAFPIAESGTVPG
                     RVQSGRARSVKEMRQVVHRPAPRAVGRCRGGRLSRARDS"
     gene            complement(3013..3513)
                     /locus_tag="AC150V_00820"
     CDS             complement(3013..3513)
                     /locus_tag="AC150V_00820"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HIR3761496.1"
                     /translation="MPDVLAPGLDVVFCGINPGRVSAAAAAHFANPRNDFWRLLREAG
                     FTPRLYAPEEQFTLPALGIGLTNAAYRTTPGSSDLRREDFAGSRERLEQLAAELHPRV
                     IAFVGKEAYRGPFGRRAEHGLQPDRVCDTALFVLPSTSPANAAVSWEERLRWFAALRE
                     LLDTDL"
     gene            complement(3530..3721)
                     /locus_tag="AC150V_00825"
     CDS             complement(3530..3721)
                     /locus_tag="AC150V_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761497.1"
                     /translation="MQRLRPLSEEECYLRCYGWIGTGEFVTVLETEAERPRETPSVLA
                     ERLRLQFESALDDRDREAA"
     gene            3775..4194
                     /locus_tag="AC150V_00830"
     CDS             3775..4194
                     /locus_tag="AC150V_00830"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR3761498.1"
                     /translation="MATVRARSFEYGVTLDSAWTLASDRGGAAIPNDEAWTPEHLLLA
                     GLSRCVLTSLTYHARRAGAEVSSSGSASGTVTRREADGRFAFVEIQVTGDVTIDPTPD
                     PDALRELLAKGERDCFVAASLAVKPSYRWVVNGVEVE"
     gene            4191..5357
                     /locus_tag="AC150V_00835"
     CDS             4191..5357
                     /locus_tag="AC150V_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HIR3761499.1"
                     /translation="MSTTLLDVTAVRARFTALDRSLAFFDGPGGTQCPDEVIDAVAAY
                     LRESNANIGGPYETSRRTDVLVEHAHARAGAFLGCSADEVAFGPSMTALNFNLTRAFG
                     RTLREGDEVVVTKLDHDANVAPWLELAHDVGIVVRHASLNDDLSLDLADLERQLSERT
                     RVVGFPVAANSVGTVPDVGRVVELAHSVGALAWADAVHFAPHGRIDVEQWDVDVLICS
                     PYKFFGPHLGLAFGKRELLESWRPYKVRPAADFPVGHRFELGTLPHELLAGFVAAVDY
                     VDSLGWDAISRHERDLGERFLAGLPQGVALYGIPSMEGRVPTFCFNVPGRSAAEVAVW
                     LGERNLAVWHGDYYAVETMRHLGLSDSGAVRAGIVHYNTPDEVDRLLAGLDELV"
     gene            5354..6319
                     /locus_tag="AC150V_00840"
     CDS             5354..6319
                     /locus_tag="AC150V_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3761500.1"
                     /translation="MRILVLGGTKFVGRAFVEAALAAGHEATLFHRGQTNSGLFPEAE
                     HVQGDRDGELDALEGRTWDACVDTCGYLPRIVRRSAELLRAALHRYVFVSSISVYADF
                     DNPPVEGAPLAQLEDPAVEEITGETYGGLKALCERAVEESYGERATLIRPGFVTGRHD
                     PTGRFTYWAQRGARGGEALAPASLAARLQAVDAHDLGAFMLRVIEDDLGGAFNVTAPM
                     PPGSIADVLHAGEAAAVSDLLVTVADDSFLVDRGVEMAELPLWAPPTEYRHLLDADVS
                     RAFAAGFAPRSLVEAATEALEAEPVAGVGLSPERESELLEAWHGR"
     gene            6309..6629
                     /locus_tag="AC150V_00845"
     CDS             6309..6629
                     /locus_tag="AC150V_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761501.1"
                     /translation="MGADKDYSGTPLSQKLGAKPGLGVLICFVTTREQLERRFLPLKD
                     TLAPADGLWIAWPKKAAKIEGDLSFEAVQEIGLANGLVDNKSCAIDERWQALRFVYRL
                     EDRP"
     gene            complement(6634..7512)
                     /locus_tag="AC150V_00850"
     CDS             complement(6634..7512)
                     /locus_tag="AC150V_00850"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR3761502.1"
                     /translation="MAGELTKAAITAQPEWLREIDQRVGDRRLPTGARVLFTGCGTSY
                     HAARACGSAAQALDVVIGAAPEADVLVAVSHEGETTFTLEALRGFHGETWLITGAEDS
                     PSGRSADNVVVATPELEKSYCHTASYTCAVAAGRALLGEDVSRLPADVEQELAAEPIE
                     ASAGNRFVLAGAGSDYATVLEGALKLREGAHVAAEAHQTEQLLHGHLAAIDESVRCFL
                     LRGPGRAAERTDDAAAALTELGCEWTVFEPVHPVVDVIRFQRLAVDLAELRGVDPDMI
                     RWDSERWDRARKSHGF"
     gene            7538..8806
                     /locus_tag="AC150V_00855"
     CDS             7538..8806
                     /locus_tag="AC150V_00855"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3761503.1"
                     /translation="MPVFEQHQLANGIRVLTAPMSHAQSVSCVVMFAAGSRYETAENN
                     GIAHFAEHMFFKGTERRPNARAISSEIDGIGGEFNAFTGKELTGYYVRCAGESRDIAL
                     DVLTDMLRHSKFDEGEIEREKGVIVEEMNMYFDTPRDYVSSVWETLVYGDQPLGWDVI
                     GRKETVRSATRETFTSYLDKWYRPERMVVGVGGAVGDGLLEQLEELVGDEEALATGTP
                     EPLVLPRNGASIRVHEKSSDQAHLVLGARSYPLGHPDRYAVQLLVAVLGGGMSSRLFT
                     EVRERRGLAYYVFATNHSYTDAGSVYARAGVDVKRVDEAVETIVSELRCAAAYPIPGE
                     ELEKARSYVKGRFVLQLESPHGIIQFGLRREVLEGKVDEPEDILAGLDAVTGEDVQRV
                     AQDILGDATLYLALIGPFDDSGRFEPLLAA"
     gene            complement(8824..9183)
                     /locus_tag="AC150V_00860"
     CDS             complement(8824..9183)
                     /locus_tag="AC150V_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761504.1"
                     /translation="MDEQKQPPPEEEQDDVEAHGLKETVAAAAAVGAIAVPSASAYAP
                     TVAPDGTSGKLAPASVQSKGATTKKKAKQASAKASAGKTSPRKPLLQPEPTGADENTA
                     AAAGKTSPKKPPLLPEG"
     gene            complement(9259..11211)
                     /gene="acs"
                     /locus_tag="AC150V_00865"
     CDS             complement(9259..11211)
                     /gene="acs"
                     /locus_tag="AC150V_00865"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR3761505.1"
                     /translation="MSEKTIEALFLEERRYPPPPAFTAQANAQPEIYDVPFDEFWETQ
                     GRERVTWFEPFVTLCSWEPPYAKWYIDGRLNVCFNCVDRHVEAGLGERTAYFWEGEPE
                     GERREITYSQLQREVVRFANVLKELGVRKGTPVAIYMGMVPELPVAMLACTRLGAPHT
                     VVFGGFSADSLSDRMNDMGCEFLLTQDEAWRRGKTVPLKRTADEAMDAAPGVRGCLVV
                     RRTGGDVPMQDGRDTWLHEYSVSEDPVDCPCEPMDAEDLLFLMYTSGTTAKPKGIVHT
                     TGGYLVGVSTTHHYIFDLKPEHDVYWCAADIGWITGHSYIVYGPLCNAATSVLYEGTP
                     DYPGFDRWWDVIERYGVTILYTAPTAIRAHMKWGAEHAAKHDLASLRLLGSVGEPINP
                     EAWVWYHENVGGGRCPVVDTWWQTETGMILITPLPGITTLKPGSAARPFPTVEAAVVS
                     EEGGEVGPGGGGYLALKNPWPAMTRGIFGDPERFMETYWSRYPGLYFAGDGARIDEDG
                     DYWLLGRVDDVMNVSGHRISTIEVESALVDHPGVAEAAVCGRADETTGQAIVAYVTLR
                     GGVDGSVVMLEELRAHVAEKIGPIAKPANIVFTPELPKTRSGKIMRRLLRDVAENRPL
                     GDTTTLADPTVVTEIAERATAEPHDE"
     gene            complement(11208..12347)
                     /locus_tag="AC150V_00870"
     CDS             complement(11208..12347)
                     /locus_tag="AC150V_00870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3761506.1"
                     /translation="MPDVLIIGATERDLDLRHEIPVAITDPFVYAEVDGTRHVWIWAF
                     EIDRIREAGVEAQFHPAEELRIEEVARTGADTYALQRELWLRAVRALGLERATVPASF
                     PVGIADHLREHGIELTVDQRFFDDRRRIKTPQELAGIRRAQRAAEAGMAAWLELARAA
                     QRRDGHLVLDGEPLTCERVKEHVVGAAVLHDCAAEEFIVSHGPQTAVGHDAGSGAIGA
                     DDVVLLDLFPRDRSSACFADMTRTFVVGSLPDEIHEYHRLAKEALELCVAEIRPGLTG
                     KELHTRVCEFFEEHGQQTQLSKPDGEVLRDGYYHATGHGVGLDVHEQPNIGRIGEPFV
                     AGDVLAIEPGLYRYGFGGVRLEDLVLVTESGCEVLTEFPYEMEIT"
     gene            12391..>13426
                     /locus_tag="AC150V_00875"
     CDS             12391..>13426
                     /locus_tag="AC150V_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3761507.1"
                     /translation="MADALSTLKERLAEIHDLSRSAGVLGWDQRVTMPPLGTEARAEA
                     LATLGRIAHERFVDAEIGRLLDELAPLEESQPYESDDASLIRVTRRDWEKARRVPPEL
                     EVEMTRAAARGHQAWVDARRNDDFASFLPYLRTNVELKRRYVECFEWSDSPYTPLLDD
                     YEPGMTTAEVAGVFSVLRPALTDLVLGAPDVDAHFLRGTFPVDRQQAFADLVLPTLGF
                     EEGSWRIDPTAHPFCTSFSNRDVRLTTRYHEQNLESVWSTLHEAGHGLYAFGIADGLQ
                     RTPLAGAPSLGLNESQSRTWENLVGRARPFWTHWYPTLQKHFPGALGSVELESFLRAI
                     NRAEPGLIRVD"
BASE COUNT         2030 a         4664 c         4569 g         2163 t
ORIGIN      
        1 gaaagggcca gcacttcgcc ggccccgccg aacagcgccg ttcccgcgaa gagtttgcgt
       61 ccgtcggagc cgatgggcca gccgacggca atgcagtgct cgccctgcaa cgggaggcgc
      121 gcgatgcgag cggtcatacg acccaggacg acctcgccgc ggtcgctcat gccgcaggcg
      181 aaggcgccgg gacagtcgat cgcggcccag accacaggct cgttgacctc gtccggtgtc
      241 cacggcgcgg caacgatgtc gctccggcct gcgatggggc ccgggaagat ccgcaggccg
      301 tcgcctacgt cgcgcgacgg gccgcagacg aagcattccg gaaaggcgtg ctcgtggaag
      361 ccgatgtagc gcgatgcggc gtcgatcgcc tcgtccggcg agacgggggc tggagctgcg
      421 acgccgaggg agccggccac ggcctccgca atgagctcgc cgtccgcggc gaggagggcc
      481 cctccgtcga ctgagtcgac gtgcagctcc tgctccagcg gtggccgact gcgtagcgtc
      541 acttcgactt caggcgcctc aacgaacgcg gcgagcaggc cgcaggtgta gccgccgttg
      601 ccggagtcgg agggcccgca atagcggcgc gcgatagtga tcgtcgcgag gcgcaccgtc
      661 tgtagactac gttttcgacc gtgagtcgca aactcgatcg accagaggtc cggcgctgag
      721 gctcgccctc gcccagctca acgtggtcgt cggcgatctg gacggcaatc gagaccgcat
      781 cgtcgacgcc ttggcggagg cgcgcggccg aggagccgac gtcgcgatct tccccgagct
      841 cgccgtcacc gggtacccgc cggaagacct cctccttcgt cccggcttcg tccgcgcagc
      901 gcgtcgcgcg ctcgaccaga tcgccgccgc atcgacgggg atgaccgcgc tcgttggcac
      961 gccctggctc gagagcgatc tcttcaacgc ctgcgcggtc tgtgcggacg gtgaggtgcg
     1021 cggcgtgtac cgcaagcact tccttccgaa ttacggggtc ttcgacgagc accgctactt
     1081 cgccgagggg cgcgacctgc tcctgctgcg cctcggcgac gcattgctcg ggcccaccgt
     1141 gtgcgaggac gtctggcagc ccggcccccc cgcgaccgat ctcgcactgg ccggcgctca
     1201 tctgctcgtg aacatctccg cctccccgtt tcacgtcggc aaggcagagg atcgcgagga
     1261 gatgctcgtg acccgggctc gggacaacac gtgctacctc gcgttctgca atctcgtcgg
     1321 cgggcaggac gagctcgtat tcgacgggca ttcggtcgtc ctcgacgacg agggggaggt
     1381 gatcgcgcgg gcgaagggct tcgaggagga gctgctcgtc gtcgacatcg acccgaccga
     1441 ggcggtggcc cgccgactcc gcgacgtccg ccggcgtgag ctcggccgga gccgcggcgg
     1501 cgctcccgcg gcgcccgtga tcgagctcgg agagcccgcc cggtcggagc ggccggtcac
     1561 agccgcgacg gcggccttcg cgccggagct cgagcagatg cgccgggcgc tcgtcctcgg
     1621 tctacgcgac tacgtgggaa agaacggctt cggcgacgtc gtcgtcgggg tctccggcgg
     1681 gatcgactcc gctgtgaccg cggccatgtg cgtcgatgca ctgggcgccg agcgcgtgca
     1741 ctgcgtctcg atgccgtctc gctacagctc gaccgaaacc cggacggacg ctcgtcgcct
     1801 ggccgacaac ctcggctgcg acttccatga gctctcgatc gaggcggcag tcgagtcgtt
     1861 ctcgactgtg ctggctgcgt cgatcgaagg gggcccatcc ggtctcgcag ccgagaacct
     1921 ccaggcccgc atccgcggga cgatgctgat ggcgctttcg aatcagttcg gctggctggt
     1981 ggtctcgacg gggaacaagt ccgagctcgc cgttggctac tcgaccctgt acggcgacat
     2041 ggtcggtggc ttcgcgctcc tgaaggacgt cttcaagacc gacgtttctc ggctcgcccg
     2101 ctacgtgaac gaacgggccg gggaggatcg gattccggtg acgaccatcg agcgtgctcc
     2161 gtccgcagag cttcgtgccg atcagctgga cgaggactcg ctgcccgctt atgaggcact
     2221 cgaccaggtg ctcgaggcct acgtcgagga ggaccgctcg cgggaggagt tgctagccgc
     2281 gttcgatccc gacgttgtgg agcgggcgct cgatctggtc gatcgcgccg agtacaagcg
     2341 ccggcaagcg ccgcccggcg tcaagctgcg cccgaaggcc ttcgggcgcg actggcgccc
     2401 cccgatcacg aatctctggc gcggctgagt cgtcccccgc ggcatcgccc cacggccctc
     2461 ggcgccggcc tgtgaacaac ttggcgcatt tcctttacgg atcgggcacg gccgctctgg
     2521 acgcgtccgg ggacagtccc cgattccgcg atcgggaaag cgaaagcgga cgaggagcac
     2581 gcggtcgtcc gggtcgagaa cgagtgtgca ggcggcggga cggagacgaa agcggtccgc
     2641 tcagagcgtg agcagcaaga tgatcgtggc gaccccgggc agtgcgcccg cgatggagag
     2701 cccgccgagc agtgccggtg cgggccgcgc atactcgccc cgccgaaccg ccacgcccac
     2761 ccgccgctgc cggtacccgc cgtagagcag gacgccgacg ccgctggcga tgtagaggga
     2821 gccggcgagc gcaaacggcc agtcgggccc gctgagaact cgatcccgag cgcaccgagg
     2881 gcaaagccgc ccgcgaccgc cccgagcccc gtccgccacc aggcgaggta ggtccgctcg
     2941 ttcgcgaggt gtgtgcgccg cgtggcgtcg acgccctcgc tcgcgctgcc ctcgggcgaa
     3001 ttcacagcgt gactatagat ccgtgtcgag cagctcccgc agcgccgcaa accagcgaag
     3061 ccgctcctcc caggacacgg ccgcgttcgc cggcgaggtc gaaggcagga cgaacagggc
     3121 cgtgtcgcac accctgtccg gctgcaaccc gtgctccgcc cggcggccga agggaccgcg
     3181 gtatgcctcc ttgccgacga acgcgatcac gcgaggatgg agctcggccg ccagctgttc
     3241 gaggcgctcg cggctgcccg caaagtcctc tcgccgcaag tcggatgacc cgggcgtcgt
     3301 ccggtacgcc gcgttggtca ggccgatccc gagcgcgggg agcgtgaact gctcctcggg
     3361 tgcatagagg cgcggtgtga agccggcctc gcgcagcagg cgccagaagt cgtttcgcgg
     3421 gttggcgaag tgggctgcgg ccgccgccga gacccgtccg gggttgatgc cgcagaacac
     3481 cacgtcaagg ccgggcgcga gcacgtcggg caccgacgac ggggtggcgc taggcagctt
     3541 cgcgatctcg gtcgtcgagc gcagactcga actggaggcg gaggcgctct gcgagcacgc
     3601 ttggcgtctc gcgtggacgc tcagcctcgg tctcgagcac cgtgacgaac tctccggtgc
     3661 caatccagcc gtaacagcgg aggtagcact cctcctcgct cagcggcctc aggcgctgca
     3721 tgcgcgcaag gtagatgccg gtccggacgg cattcggcgt atacgctgcc gctgatggcc
     3781 accgttcgtg cgcggagctt cgagtacggc gtcacgctcg actccgcctg gactctcgcg
     3841 agtgaccgtg gcggtgcggc gatccccaat gacgaggcgt ggactccaga gcatctcctc
     3901 cttgccgggc tgagccgctg cgtgctcacg agcctcacgt atcacgcccg gcgcgcagga
     3961 gcggaggtct cctcgagcgg gtctgcctcg ggcacggtga cacgccgcga ggcggacggc
     4021 cgtttcgcct tcgtcgagat ccaagtcacc ggcgatgtca cgatcgatcc cactcccgac
     4081 cccgatgcgc tccgcgagct ccttgcgaaa ggagagcgcg actgtttcgt cgcggcctcc
     4141 ctcgcggtca agccttcgta ccgctgggtc gtgaacggag tggaggtgga atgagcacga
     4201 cgctgctgga cgtcaccgcc gtgcgcgccc gcttcactgc gctcgaccgg tcgctggcgt
     4261 tcttcgacgg tccgggcggc actcagtgcc ccgacgaggt gatcgacgcg gtcgcggcct
     4321 accttcgcga gtcgaacgcc aacatcggcg ggccgtacga gacatcgcgt cgaaccgacg
     4381 tccttgtcga gcacgctcac gcccgggcag gcgcctttct cggctgcagt gccgacgagg
     4441 tcgccttcgg accgagcatg accgccctca acttcaatct cacgcgcgcc ttcgggcgca
     4501 cgttgcggga aggcgacgag gtcgtcgtga ccaagctcga ccacgatgcg aacgtcgcgc
     4561 cgtggctcga gctcgcgcac gacgtcggca tcgtcgtgag gcacgcctcc ctcaacgacg
     4621 atctctcgct cgacctcgcc gatctcgagc gccagctgag cgagcgcaca cgagtggtcg
     4681 ggttcccggt cgcagcgaac tccgtcggaa ccgtgcccga cgtcggccgg gtggtcgagc
     4741 tcgcgcactc ggtgggcgca ttggcctggg cggatgccgt tcactttgcg ccgcacggac
     4801 ggatcgacgt cgagcagtgg gatgtcgacg tcctcatctg ctccccgtac aagttcttcg
     4861 ggccgcatct ggggctcgcc ttcggcaagc gagagctgct ggagtcgtgg cggccctaca
     4921 aggtgcgccc ggctgcggac ttccccgtcg ggcaccgctt cgagctgggc acgctcccgc
     4981 acgagctgct cgcggggttc gtggctgccg tcgactacgt cgactcgctc ggctgggatg
     5041 cgatatcccg ccacgagcgc gatctgggcg agcgcttcct cgccggtctt ccccagggtg
     5101 tcgccctcta cgggatcccg tcgatggagg gccgcgtgcc gacgttctgc ttcaacgtcc
     5161 ccggtcgatc ggcggcggag gtcgcggtat ggctcggtga gaggaatctc gccgtctggc
     5221 acggcgacta ctacgcggtc gagaccatgc gccacctcgg gctctccgac agcggcgcgg
     5281 tgcgcgccgg gatcgtccac tacaacaccc cggacgaagt cgaccggctc ctggccggac
     5341 tggacgagct cgtgtgagga tcctcgttct cggcggaacg aaattcgtcg ggcgcgcatt
     5401 cgtcgaggct gcgcttgccg ccgggcacga agcgacgctc ttccatcgag gacagacgaa
     5461 ctcaggactc ttccccgaag ccgagcacgt tcaaggcgac cgggacggtg agctcgacgc
     5521 gctcgagggc cgcacgtggg acgcctgcgt cgacacctgc ggctacctcc cccgcatcgt
     5581 ccggcgctcg gcggagctgc tgcgagccgc tctccatcgc tacgtcttcg tctccagcat
     5641 ctccgtgtac gcggatttcg acaacccgcc cgtcgaagga gctcccctcg cacagctcga
     5701 ggatcccgcg gtcgaggaga tcacgggaga gacctacggc gggctgaagg cgctctgcga
     5761 gcgtgccgtc gaagagagct acggggagcg ggcgacgctg atcaggccgg gattcgtcac
     5821 cggtcgtcac gacccgacag gccggttcac gtactgggcc cagcgcggcg cccgaggcgg
     5881 cgaggcgctc gcgcccgcct cgttggcggc gcgtctccag gcggtcgacg cgcatgacct
     5941 cggtgccttc atgctgcggg tgatcgagga cgacctcgga ggtgcgttca acgtgaccgc
     6001 gccgatgccg cccggcagca tcgcggatgt cctgcatgcc ggggaggccg ccgccgtctc
     6061 cgacctgctc gtgaccgttg ccgacgactc ctttctcgtc gaccgcggcg tcgaaatggc
     6121 cgagcttccg ctctgggctc cgccgaccga gtaccggcat ctcctcgacg ccgacgtctc
     6181 gcgggccttc gcagcagggt tcgctccccg gtcgctcgtg gaggcggcga ccgaagcact
     6241 cgaagccgag cccgtggccg gagtgggcct gtcgcccgag cgcgagagtg agctgctcga
     6301 ggcctggcat gggcgctgac aaggactact ccgggacgcc gctttcgcag aagctcggcg
     6361 cgaagccggg cctcggtgtc ctcatctgct tcgtgacgac ccgtgagcag ctcgagcgtc
     6421 gattcctgcc gctgaaggac acgctcgcac cggctgacgg gctgtggatc gcgtggccca
     6481 agaaggcggc gaagatcgag ggcgacctca gcttcgaggc ggtgcaagag atcgggctcg
     6541 ccaacggact cgtcgacaac aagagctgcg cgatcgacga gcgctggcag gcgcttcgct
     6601 tcgtctaccg gctcgaagac agaccctgag ccgctagaac ccgtgcgact tgcgggcccg
     6661 gtcccaccgc tccgaatccc accggatcat gtccgggtcg acgccgcgca gctcggcgag
     6721 atcgactgcg agccgctgga aacggatgac gtccaccacc ggatggacgg gctcgaagac
     6781 cgtccactcg catccgagct cggtgagtgc ggccgccgcg tcgtccgtgc gctcggccgc
     6841 gcggccgggt cctctcagca ggaagcagcg aaccgactcg tcgatcgcgg cgaggtgtcc
     6901 atgcaggagc tgctcggtct ggtgcgcctc ggcggccaca tgggcgccct cgcgcagctt
     6961 gagtgctcct tcgaggacgg ttgcgtagtc ggaacccgcg cccgcgagaa cgaagcgatt
     7021 gcctgcggac gcctcgatcg gctccgcggc gagctcctgc tcgacgtccg cggggagccg
     7081 actcacgtcc tcgcccagca aggcgcgacc cgccgccacg gcgcacgtgt agctggccgt
     7141 atggcagtag ctcttctcga gctccggtgt cgcgacgacg acgttgtcgg cgcttctgcc
     7201 gctcggcgag tcctcggcac cggtgatgag ccaggtctcg ccgtgaaatc cccttagcgc
     7261 ctcgagcgtg aaggttgtct ccccctcgtg gctcaccgcc acgagcacgt ccgcctccgg
     7321 cgcggccccg atcacgacat cgagggcctg cgccgcgctg ccgcaggcac gcgcagcgtg
     7381 gtacgaggtg ccgcagcccg tgaacagcac ccgggcgccg gtcgggagcc gccggtcacc
     7441 cacccgctgg tcgatctctc ggagccactc gggctgagcc gtgatcgccg cttttgtcag
     7501 ctcgccggcc atagacccgt ctataccatc atcttccgtg ccggtcttcg agcagcacca
     7561 gctcgccaac gggatccgag tcctgacggc gccgatgtcc cacgcgcagt cggtctcctg
     7621 cgtcgtgatg ttcgcggccg gatcgcggta cgagaccgca gagaacaacg gcatcgccca
     7681 cttcgcagag cacatgttct tcaaggggac ggagcgtcgc ccgaacgcgc gggccatctc
     7741 gagcgagatc gacggcatcg gcggcgagtt caacgccttc accggcaagg agctgaccgg
     7801 ctactacgtt cgctgcgccg gcgagtcgcg cgacatcgcg ctcgatgtcc tcaccgacat
     7861 gctgcggcac tcgaagttcg acgagggcga gatcgagcga gagaagggcg tgatcgtcga
     7921 ggagatgaac atgtacttcg acacgcctcg cgactatgtg tcgagtgtgt gggagaccct
     7981 ggtctacggc gatcagcccc tcggctggga cgtgatcggg cgcaaggaga ccgttcgctc
     8041 ggcgacgcgc gagacgttca cctcctacct cgacaagtgg taccggccgg agcggatggt
     8101 ggtcggagtc ggcggcgcag tcggcgacgg cctgctcgaa caactcgagg agctggtggg
     8161 cgacgaggaa gctctcgcga ccgggacgcc cgagccgctc gtgctgcccc ggaacggcgc
     8221 atcgatcagg gtgcacgaga agtcgtccga ccaggcacac ctcgtgctcg gggcacgcag
     8281 ctacccactc ggccatccgg accgctacgc agtccagctg ctcgtcgcgg tgctcggtgg
     8341 gggcatgtcg tcacggctgt tcaccgaggt gcgtgagcgg cgcggtctcg cctactacgt
     8401 gttcgctacg aaccacagct acacggacgc cggctccgtg tacgcacgcg cgggtgtcga
     8461 cgtcaagagg gtcgacgagg cggtcgagac gatcgtctcg gagctgcgct gcgccgccgc
     8521 ctatccgatc cccggcgagg agctcgagaa ggcgcgctcc tacgtcaagg gtcgcttcgt
     8581 cctccagctc gagagccccc acgggatcat ccagttcggg ctccggcggg aggtcctcga
     8641 gggaaaagtc gacgagccag aggacatcct ggcgggactg gacgcggtca ccggcgagga
     8701 cgtgcagagg gtggcccagg acatcctggg tgacgccacg ctgtacctgg ccctgatcgg
     8761 gccgttcgac gattcgggcc ggttcgagcc cctgctcgcc gcctgatccc gcgccccgcg
     8821 gcttcagccc tccggcagca gcggcggctt cttcgggctc gtcttcccgg ccgcggccgc
     8881 cgtattctcg tcggcaccgg ttggctccgg ctgcaacaga ggcttccgcg gcgacgtctt
     8941 gcctgcggac gccttggcgg acgcttgctt cgccttcttc ttcgtggtcg cacccttgct
     9001 ctgcacgctc gccggtgcga gcttccccga cgttccgtcg ggagcgaccg tcggggcata
     9061 cgcactcgca ctggggactg cgatggcgcc gaccgccgcg gcggccgcca ctgtctcctt
     9121 caggccgtgg gcctcgacgt cgtcctgctc ttcctcgggg ggtggctgct tctgctcgtc
     9181 catgtcgtcc tccctttggg gtctcgacta ccagacgtgc tatgcacgag gaatcttccc
     9241 tgactgcgag gtcgatggct actcgtcgtg cggctcggcc gtcgcgcgct cggcgatctc
     9301 ggtgacgact gtggggtcgg cgagggtcgt cgtgtcgccc agcgggcggt tctcggcaac
     9361 gtcacgaagc aggcgacgca tgatcttccc ggagcgcgtc ttcggcagct cgggggtgaa
     9421 gacgatgttc gccggcttcg cgatcggtcc gatcttctcg gccacgtgag cgcgaagctc
     9481 ttcgagcatc accaccgagc cgtcgacacc tccccgcagc gtcacgtagg cgacgatcgc
     9541 ctggcccgtc gtctcgtcgg cgcggccgca caccgccgct tcggcaacgc cggggtggtc
     9601 gaccagcgcg gactcgacct cgatcgtcga gatccggtgg ccggagacgt tcatgacgtc
     9661 gtctacgcgc ccgagcagcc agtagtcgcc gtcctcgtcg atgcgcgcgc cgtcgcccgc
     9721 gaagtagaga ccgggatacc gagaccagta cgtctccatg aatcgctcgg ggtcaccgaa
     9781 gatcccacgc gtcatcgccg gccacggatt cttgagcgcg aggtagccgc ccccgcccgg
     9841 gccgacctcg ccgccctcct cgctcaccac ggcggcctcg acggtcggga agggccgggc
     9901 tgccgaaccg ggcttcagcg tcgtgatccc gggcagcgga gtgatcagga tcatccccgt
     9961 ctcggtctgc caccacgtgt cgacgacggg gcagcgacct ccaccgacgt tctcgtggta
    10021 ccagacccat gcctcggggt tgatcggctc gccgaccgat ccgagcagcc gcaggctggc
    10081 caggtcgtgt ttggccgcgt gctccgcgcc ccacttcatg tgcgcacgga tcgcggtggg
    10141 ggccgtgtag aggatcgtca cgccgtagcg ctcgatcacg tcccaccagc ggtcgaagcc
    10201 cggatagtcg ggcgttccct cgtacaggac gctggtcgcg gcgttgcaga gaggcccgta
    10261 gacgatgtag ctgtggcccg tgatccagcc gatgtcggcc gcgcaccagt acacgtcgtg
    10321 ctcgggcttc aggtcgaaga tgtagtggtg ggtcgtcgag acgccgacca gatagccgcc
    10381 ggtcgtatgg acgatcccct tcggcttcgc ggtcgtgccc gacgtgtaca tgaggaagag
    10441 cagatcctcg gcgtccatcg gctcgcacgg gcagtcgacc ggatcttcgg acacggagta
    10501 ctcgtggagc caggtgtcgc gcccgtcttg catcggcacg tcgccgcccg tgcggcggac
    10561 gacgaggcac ccgcggacgc ccggggccgc gtccatcgcc tcgtccgccg tgcgcttcaa
    10621 gggcaccgtc ttgccacgcc gccaggcctc gtcctgggtg agaaggaact cgcagcccat
    10681 gtcgttcatc cggtccgaga gcgagtccgc cgaaaacccg ccgaagacga cggtgtgcgg
    10741 agcaccgagg cgagtgcagg ccagcatggc gacgggaagc tcgggaacca ttcccatgta
    10801 gatcgcgacc ggcgtcccct tgcgcacgcc gagctccttg agcacgttcg cgaaccggac
    10861 gacctcccgc tggagctgtg agtaggtgat ctcccgccgc tccccctccg gctcgccctc
    10921 ccagaaatac gccgtccgct cgccgaggcc ggcctcgacg tgacggtcga cgcagttgaa
    10981 gcagacgttc aagcggccgt cgatgtacca cttcgcgtag gggggctccc aagagcagag
    11041 agttacgaac ggctcgaacc aggtgacccg ttctcggccc tgtgtctccc agaactcgtc
    11101 gaaggggacg tcgtagatct caggttgggc gttcgcctgc gcggtgaacg cagggggagg
    11161 gggataccgg cgctcctcga gaaacagggc ctcgatcgtc ttctcgctca cgtgatctcc
    11221 atctcgtagg ggaactcggt caggacctcg cagccgctct cggtgaccag cacgaggtct
    11281 tcgagtcgta cgccaccgaa gccgtatcgg tacaggcccg gctcgatcgc cagcacgtcc
    11341 ccggcaacga acggctcgcc gatccggccg atgttcggct gctcgtgcac gtccagcccg
    11401 accccgtgtc cggtcgcgtg gtaatagccg tctcggagga cctcgccgtc cggtttcgac
    11461 agctgagtct gctgtccgtg ctcctcgaag aactcgcaca cgcgggtgtg gagttctttg
    11521 ccggtcaggc cggggcggat ctccgcaacg cagagttcga gcgcttcctt cgccagccgg
    11581 tggtactcgt ggatctcgtc cggcagcgat ccgacgacga acgtcctcgt catgtcggcg
    11641 aagcacgcag aggagcggtc gcgcggaaac aagtcgagca gcacgacgtc gtcggcgccg
    11701 atcgccccgg acccggcgtc gtgcccgacc gcggtctgcg gcccgtgcga gacgatgaac
    11761 tcctcggcgg cgcagtcgtg cagcacggct gcaccgacga cgtgctcttt gacgcgctca
    11821 caggtcagcg gttcgccgtc gagaacgaga tgtccgtccc gtcgctgcgc ggctcgggcg
    11881 agttcgagcc aggccgccat gccggcctct gcggctcgct gtgcccgccg gatccccgcg
    11941 agctcctgcg gcgtcttgat ccggcgtcgg tcgtcgaaga accgctgatc gacagtcagc
    12001 tcgatcccgt gctcgcggag gtggtcggcg atcccgaccg ggaatgacgc aggcactgtc
    12061 gcccgttcga ggccgagcgc gcgcaccgcc cggagccaga gctcccgctg cagcgcgtac
    12121 gtgtcggctc ccgtcctcgc aacctcttcg atgcggagct cctcggcggg gtggaactgt
    12181 gcttcgacgc cggcctcgcg aattcggtcg atctcgaaag cccagatcca cacgtgtcgt
    12241 gtgccgtcca cttccgcgta gacgaagggg tccgtgatcg cgacagggat ctcgtgccgt
    12301 agatcgaggt cacgctcggt cgctccgatg atcaacacgt ccggcacgtc ggcgttccat
    12361 cctagagcgt ccggtcggta gggtgccggc gtggccgacg cgctgagcac tctgaaggag
    12421 cgcctcgccg agatccacga tctctcccgc tcggcgggag tgctcgggtg ggatcagagg
    12481 gtcacgatgc caccgctcgg gaccgaggct cgtgcggaag cgctcgcgac actagggcgt
    12541 atcgcgcacg agcgcttcgt cgatgcggag atcgggagac tgctcgacga gctcgccccg
    12601 ctggaggagt ctcagcccta cgaatccgac gacgccagct tgatccgcgt gacgcgacgt
    12661 gactgggaga aggcacggcg cgtgcctccc gagctggaag tcgagatgac tcgcgccgca
    12721 gcccgtggac accaggcctg ggtcgatgcg cgccgaaacg acgacttcgc gagctttctg
    12781 ccctatctac ggacgaacgt cgagctgaag cggcgctacg tcgagtgctt cgagtggtcg
    12841 gactctccgt acacaccgct gctggacgac tacgagccgg gcatgacgac cgccgaagtg
    12901 gcaggcgtct tctcggtact ccggccggcc ctgaccgacc tcgtcctcgg cgcgcccgac
    12961 gtggatgccc acttcctgcg cggcacgttc cccgtcgacc ggcaacaggc tttcgccgat
    13021 ctcgtcctgc cgacactcgg gttcgaagag ggctcatggc ggatcgatcc gacagctcac
    13081 cccttctgca cgtccttctc caaccgcgac gtccgtctga cgacccgcta ccacgagcag
    13141 aacctggagt ccgtctggtc gacgttgcac gaggccgggc acggcctgta cgccttcggc
    13201 atcgccgacg gcctccagcg gacaccgctg gccggcgcac cctcgctcgg gctgaacgag
    13261 tcccagagcc gcacctggga gaacctcgtc gggcgcgccc ggccgttctg gacgcactgg
    13321 tatccgacgc tccagaagca cttcccgggg gcgctcgggt cggtcgagct ggagtcgttc
    13381 ctgcgggcga tcaaccgcgc cgagccgggt ctgatccgag tcgatg
//
LOCUS       DBNGNK010000032         6230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1867552,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000032 DBNGNK010000000
VERSION     DBNGNK010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6230
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1867552"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="AC150V_00880"
     CDS             <1..852
                     /locus_tag="AC150V_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR3761508.1"
                     /translation="ILTCWKQVPSAALDASPRCLVVSRYGIGLDNIPVEQATRLGILV
                     TNVPDFCLEEVSDHAMALVLACARRIVGFARATREGAWELAQASGLPRLRGQTLGLIG
                     YGGIARALVPKAQGFGLRVLAYTPRLAPGRLPDGAVATNELGALLEESDYVSLHLPAT
                     PETAGFIGPAELDRMKSSAYLVNTSRGALVDEDALVAALREGRIAGAALDVLAAEPPG
                     PDHPLLALDNVIATPHAAFYSEAAIEELAKKAAGNVAAVLRGDLPESVVNPEVLALPV
                     CRFEAGR"
     gene            881..1642
                     /locus_tag="AC150V_00885"
     CDS             881..1642
                     /locus_tag="AC150V_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR3761509.1"
                     /translation="MRSSGTALPAGHAEPCAKRVRIVFGGETVAESEATWLVWEHGSY
                     PQYYFPRTDVALDRLVPSDRRTHHATLGEAVHFSVVAGGRTATSAAWQYEDSPVVAVR
                     ELLRFDWAAMDGWYEEDEEVFVHPRDPYTRVDVLDSSRHIRVKIDGVVVAESTRPRLL
                     FETRLPTRIYLPPADVRRELCTATEKTTDCPYKGTARYWSVEVGGVVHPDIVWSYPDP
                     VPEAGKIAGLLCFYNERVDLVVDGELQPRPVTKFG"
     gene            complement(1868..2014)
                     /locus_tag="AC150V_00890"
     CDS             complement(1868..2014)
                     /locus_tag="AC150V_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761510.1"
                     /translation="MAPRLRDDGLGSKFGWALATCASNAAWASEVGSSPEFHRHMQHL
                     VVID"
     gene            complement(2101..3006)
                     /locus_tag="AC150V_00895"
     CDS             complement(2101..3006)
                     /locus_tag="AC150V_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3761511.1"
                     /translation="MPRVLLVLVTAAAVAIGTAGAGPAFPKRIDLPDGFQPEGIATAA
                     QTFYAGSIATGAVYRGNLRTGKGAVLVKARSGRQAIGLKADRGRLFVAGGPTGMAYVY
                     SAKTGAPIAAYRLATGNTFVNDVVVTRTAAWFTDSFRPVLYRVPLGAGGRPGKQTGVK
                     TVALRGELVYQEGFNANGIDATPNGKTLVLVQSNTGRLFTVAASSGAAQAIDLGSESV
                     PGGDGILLAGSTLYVVQNSSNVVAKISLRSGLRAGRVVKRIGDPQLDVPTTVAALGSR
                     LYAVNARFGTAPAASTESWVTQLKK"
     gene            3139..5202
                     /locus_tag="AC150V_00900"
     CDS             3139..5202
                     /locus_tag="AC150V_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3761512.1"
                     /translation="MSAYPHLFTPLEIGNVTVRNRILQTAHVKLFAQNAVDSQRNVDY
                     QVARAKGGAGLLITGNRVVHPTSTTGFPRVAWAFLDGAVEANRRLTQAVHEHGAIIFE
                     QLNHFGLNASSDSADDLRVLWGPSAVKSPAYGETPKAMEEEDIGDLVDWWGQCAALSR
                     EGGFDGTEVHISHSYLLHQFLSPLYNKRTDDYGGSFENRLRLAREVIAEVRRCVGTDW
                     VVGVRISLSDFIPGALDIDDAVRVAKELEQDGEIDYVNVTAAGYHNIFKAIEPADTAD
                     GYLVGLAAAVKDGVEGLPVFTIGGIKDPALAEEVLATGKADMVAMTRAQIADPEFANK
                     AREGREDEIVHCIRGNQGCIGRVFKGLAISCTVNPVAGREGRLGSLSPAQTSTRWLVA
                     GGGPAGMKAAETLAMRGHSVVLAEREAELGGQVNLIVRAPGREEFGWIRRDLARSLDR
                     LGVEVRLGTEASMQLVEELEPDGVIVATGAIPSRSGFSSVNPLAESLPGAEQENVVTA
                     WDVLRGSAAVGERVVVLDDDGGRYAAGVAEVLLDQGKSVELVSRFNALFPATLFTLDM
                     AIVYSRLLGKGLTSQLNSWAKEIDGNALTIFNLYTGAELTLRDVDTVVLATGAKADDA
                     LYFSLKGTISNLHRIGDCLAPRKLDHAIYEGYMAGREAWYPEERYIYEGQLEQWEEAS
                     IGVDV"
     gene            complement(5247..>6230)
                     /locus_tag="AC150V_00905"
     CDS             complement(5247..>6230)
                     /locus_tag="AC150V_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3761513.1"
                     /translation="HPDRVLGLVLHSSYARVLRADDFPIGLPPELWPKAVEVAGAEWG
                     RPELLGRVLPSVAGDEHRTRWWCDFFRRSSSPGAAIAQIRMQEDGDVRHVLPVVQTPT
                     LVLHATGDQWVRVAHGRYLADHIAGAQLVEFLDSDHLPYGDHADLAAAEIQEFATGVR
                     EPAAPERVLSTLLVSDIVSSTKVLADVGDERWHEILDTHDRAVRRQLERYRGREIKTT
                     GDGFLAAFDGPARAIRCAEAIRDATRAIGITVRVGLHTGEVELRDGDVAGLAVHISER
                     VCAAADGGEILVSAALPMLVAGSGIAFTDRGAHALKGLPDTWQLFSVGAPA"
BASE COUNT         1009 a         2135 c         2152 g          934 t
ORIGIN      
        1 atcctcacct gctggaagca ggttccgagc gcggcgctgg acgcttctcc gcggtgcctc
       61 gtcgtctccc gctacggcat cgggctcgac aacatcccgg tcgagcaagc gacccgtctc
      121 ggcatcctcg tcaccaatgt ccccgacttc tgcctcgagg aggtgtccga ccacgcgatg
      181 gcgctcgtgc tcgcctgtgc gcggcggatc gtcggcttcg cccgtgcgac gcgagaggga
      241 gcatgggagc tggcgcaggc ttcgggcctc ccgcgcctgc gcgggcagac gctcggactc
      301 atcggctacg ggggaatcgc ccgggcgctc gtcccgaagg cacagggctt cggcctgcgt
      361 gtgctcgcgt acacgccgag gctcgcgcca ggtcgcctgc cggacggtgc ggtggcgacg
      421 aacgagctgg gcgcgctcct cgaggagtcc gattacgtct cgctgcatct gcccgcgacg
      481 cccgagacgg ccggattcat cgggccggcg gagctcgacc ggatgaagtc gagcgcctac
      541 ctcgtcaaca cgtctcgagg cgcgcttgtc gacgaggatg ccctggtcgc cgcgctgcgc
      601 gaggggcgca tcgcgggggc ggcactggac gtcctcgccg ccgagccgcc cggccccgac
      661 catccacttc tggcgctcga caacgtgatc gccaccccgc acgccgcctt ctattcggag
      721 gcggcgatcg aagagctcgc caagaaggcg gccggaaacg tcgcagccgt gctgcgcggc
      781 gatcttcccg agagcgtcgt caaccccgag gtgctcgcgc tgcctgtctg tcgcttcgaa
      841 gccggccgtt agccgcgccg gtgcaggcat catcggtgct gtgcggagta gcggcacagc
      901 actgccggcg ggccacgcgg agccctgcgc caagcgggtt cggatcgtct tcggcgggga
      961 gacggtcgcc gagagcgagg cgacctggct ggtgtgggag cacggctcct atccgcagta
     1021 ctacttcccg cgcacggacg tcgccctcga ccggctcgtc ccgagcgatc gccgcacgca
     1081 ccacgccacg ctcggggagg cggtgcactt ctccgtggtg gcgggcggtc gcacggcgac
     1141 ctccgcagcc tggcagtacg aagactcgcc ggtggtggcg gtgcgcgagc tgcttcggtt
     1201 cgactgggcc gccatggacg gctggtacga agaggacgag gaggtcttcg tccatccccg
     1261 cgatccctac actcgcgtcg acgtcctcga ctccagtcgg cacatccggg tcaagatcga
     1321 cggagtcgtc gtcgccgaga gcacgcggcc gcgactgctc ttcgagacgc gattgccgac
     1381 ccgcatctac ctgccgccgg ccgacgtgcg gcgcgagctg tgcacggcga ccgagaagac
     1441 caccgactgt ccgtacaagg gaaccgcgcg ctactggtcg gtcgaggtcg gaggtgtcgt
     1501 ccatcccgac atcgtctggt catacccgga tccggtgccg gaagcgggaa agatcgcggg
     1561 cctgctgtgc ttctacaacg agcgggtcga cctggtcgtc gacggcgagc tgcaaccccg
     1621 gccggtcacg aagttcggct gaaccagggc gtgcgcttcg caagcgggtg gtgggcccaa
     1681 actctggacc tgacatccga gaggacgcgg cctacggtgc gagggcggaa gacgccggtc
     1741 ggcgtcgaaa aggggctggt gggtcaccag ccgagaagcg aggcgatcga gtgagcgaga
     1801 agaaggcggc agagaagaag acgttccagc aaaacgccga agcccggcgc gtagcggagt
     1861 tcgtcgctca gtcgatgacc acgaggtgct gcatgtgccg gtggaactcg ggcgagctgc
     1921 cgacctccga ggcccatgcg gcattcgagg cgcacgtcgc caaggcccac ccgaacttcg
     1981 agccgaggcc gtcgtcgcgc aggcgagggg ccaagtcgcg ttcggatcgt ccgaagcgga
     2041 acgccgggcg gaaacagccc acgcgggcat agcgcccgag ccgtcgggtc gcgggcgtgg
     2101 ctacttcttc agctgggtca cccaggattc ggtcgacgcc gccggtgccg ttccgaagcg
     2161 tgcattgacc gcgtagagac ggctgccgag tgcggcgacg gtcgtcggga cgtccagctg
     2221 cgggtcgccg atgcgcttga caacccttcc ggcccgcaac cccgagcgca gcgagatctt
     2281 ggcgacgacg ttcgacgagt tctgcacgac gtagagcgtg gagcctgcga gcaggatccc
     2341 atcgccgccc ggcacgctct cggagccgag gtcgatcgcc tgcgcagcgc cgctcgaagc
     2401 ggcgacggtg aagagcctgc ccgtgttgga ctggaccagc acgagcgtct tcccgttcgg
     2461 cgtcgcgtcg atgccgttcg cgttgaaccc ctcctgatag acgagctctc cgcgcaacgc
     2521 aaccgtcttc actccggtct gcttgccagg gcggccgccg gcaccgagcg gtacgcggta
     2581 gaggacgggg cggaacgagt ccgtgaacca tgcggctgtc ctcgtgacga cgacgtcgtt
     2641 cacgaacgtg ttgcccgtcg ccagacggta cgcggcgatc ggcgcacccg tcttcgcgct
     2701 gtagacgtag gccatcccgg tcggcccgcc ggcgacgaag agacggccac ggtcagcctt
     2761 caggccgatc gcctgacgcc ctgaccgtgc cttgacgagc acggcgccct tgccggtgcg
     2821 gaggttccca cggtagacgg caccggtggc gatcgagccc gcgtagaacg tctgtgccgc
     2881 cgtcgcgatg ccttcgggct ggaagccgtc cggaaggtcg attcgcttgg gaaacgccgg
     2941 tcccgcaccc gcagtcccaa ttgcgactgc ggctgccgtg acgagcacca gcaggactcg
     3001 gggcaccgcg cccacctcac ttccggatcg cttccatccg aaacggtaca gcgcacgcct
     3061 ggcccgggcg cgttgaagcc ccgaagtgcc gggcgtagag tggctatcac ccggccagtc
     3121 cgcacgaagg aggcgtccat gtccgcctac ccccatctct tcacccctct cgagatcggg
     3181 aacgtcaccg tccggaatcg catcctgcaa acggcgcacg tcaagctgtt cgcgcagaac
     3241 gcggtcgact cccagcggaa cgtggactac caggtcgccc gtgccaaggg cggtgcgggg
     3301 ctcctcatca ccggcaaccg cgtggttcac ccgacgtcga cgaccggctt tccccgtgtt
     3361 gcgtgggcgt tcctggacgg cgccgtcgag gccaatcggc ggctgaccca ggcggtgcac
     3421 gagcacggcg cgatcatctt cgagcagctg aaccacttcg ggctgaacgc ttcgagcgac
     3481 tcggccgacg atctccgcgt gctctggggt ccctctgcgg tcaagtcgcc ggcgtacggc
     3541 gagacgccga aggcgatgga ggaagaggac atcggcgacc tcgtggactg gtgggggcaa
     3601 tgcgcggccc tctcgcgcga gggcggcttc gacggcaccg aggtgcatat ctcgcacagc
     3661 tacctgctgc accagttcct ctcgcccctc tacaacaagc gcaccgacga ctacggcggc
     3721 tcgttcgaga accggctccg gctcgcgcgc gaggtcatcg cggaggtgag gcgctgcgtc
     3781 ggcaccgact gggtggtcgg cgttcggatc agcctgagcg acttcatccc cggcgcgctc
     3841 gacatcgacg acgccgtccg tgtcgccaag gagctcgaac aggacggcga gatcgactac
     3901 gtcaacgtca cggccgccgg gtaccacaac atcttcaagg cgatcgagcc cgccgacacg
     3961 gcggacggct acctcgtcgg gctggccgcg gcggtgaaag acggtgtcga gggcctgccc
     4021 gtcttcacga tcggcggcat caaagatccc gcgctcgccg aagaagtgct cgcgaccggc
     4081 aaggcggaca tggttgcgat gacgcgggcg cagattgccg acccggaatt cgccaacaag
     4141 gcgcgggagg gacgagagga cgagatcgtc cattgcatcc gtggcaacca gggctgcatc
     4201 ggccgcgtct tcaaggggct cgccatcagc tgcaccgtca accccgtcgc cggacgcgag
     4261 ggccgcctcg gctcactgtc accggctcag acgtccaccc gctggctcgt cgccggcggc
     4321 ggcccggcgg ggatgaaggc ggccgagacg ctcgcgatgc gcggacacag tgtcgtgctc
     4381 gcggagagag aggccgagct gggcggtcag gtgaacctga tcgtgagggc tcccgggcgt
     4441 gaggagttcg gctggatccg gcgtgacctc gcgcgcagcc tcgaccggct cggcgtggag
     4501 gtcagactcg gcaccgaggc atcgatgcag ctcgtcgagg agctcgagcc cgatggcgtg
     4561 atcgtcgcaa ccggcgcgat tccctcccga agcggcttct cgagcgtcaa cccgctggcg
     4621 gaatccttgc ccggggccga gcaggagaac gtcgtcacag cctgggacgt gctccggggc
     4681 tcggccgctg tcggcgagcg ggtcgtcgtc ctcgacgacg acggcgggcg ctacgcggcg
     4741 ggcgtggccg aggtcctgct cgaccagggc aagagtgtcg agctcgtgag ccgtttcaac
     4801 gcgctctttc cggcgacgct cttcacgctg gacatggcga tcgtctacag ccgcctgctc
     4861 ggcaagggcc tcacctcgca gctcaactcg tgggcgaagg agatcgacgg caacgccctc
     4921 acgatcttca acctctatac gggcgccgag ctcacgctca gggatgtcga tacggtcgtg
     4981 ctcgcgacgg gcgcgaaggc cgacgacgcc ctctacttct cgctgaaggg cacgatctcg
     5041 aatcttcacc gcatcggcga ctgcctcgcg ccccgcaagc tcgatcatgc gatctacgag
     5101 ggatacatgg cgggtcgcga agcctggtac ccggaggagc gctacatcta cgagggccag
     5161 ctcgagcagt gggaagaggc gtcgatcggc gtagacgtgt agcgacgcct cgctcacaag
     5221 agagcgccgc accctcgaga cacggctcaa gcgggagcgc cgaccgagaa cagctgccag
     5281 gtgtcgggca gccccttgag cgcgtgcgcg ccgcgatccg tgaacgcgat ccctgaccct
     5341 gccaccagca ttgggagcgc ggccgagacg aggatctcgc ctccatccgc cgccgcgcag
     5401 acgcgctcgc tgatgtggac cgcgagaccc gccacgtcgc cgtcacggag ctccacctcg
     5461 ccagtgtgga ggccgacccg caccgtgatg ccgatcgccc gcgtcgcgtc ccggatcgcc
     5521 tcggcgcagc ggatggcgcg cgctgggccg tcgaacgcgg cgaggaagcc gtcacccgtt
     5581 gtcttgatct cccggccgcg gtaccgctcc agctgccgtc gcacggcccg atcgtgcgta
     5641 tcgaggatct cgtgccagcg ctcgtcgccc acgtcggcga gcaccttggt cgaggagacg
     5701 atgtcgctga ccagcagagt cgagagaact cgctcgggcg cggccggctc ccgcacgccg
     5761 gtcgcgaact cctggatctc ggcggcggcg agatcggcgt ggtccccgta cggaaggtgg
     5821 tcgctgtcga gaaactccac gagttgcgcg cccgcgatgt gatccgcgag gtagcggccg
     5881 tgcgccactc gaacccactg atcgccggtc gcgtggagaa ccagcgttgg ggtctggaca
     5941 acgggcagga catgcctgac gtcgccgtcc tcctgcatcc ggatctgggc gattgcggct
     6001 cccgggctgc tcgaacgccg gaagaagtca caccaccatc tcgtgcggtg ctcgtcgccg
     6061 gcgacgctcg ggagcacgcg gccgagcagc tcaggccgac cccactctgc accggctacc
     6121 tcgaccgcct tcggccagag ctcgggtgga aggccgatcg gaaagtcgtc cgcacgtagt
     6181 acgcgggcgt acgacgaatg cagaaccaag ccgagtacgc gatccggatg
//
LOCUS       DBNGNK010000033         4744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1366175,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000033 DBNGNK010000000
VERSION     DBNGNK010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1366175"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="gpmI"
                     /locus_tag="AC150V_00910"
     CDS             complement(<1..1000)
                     /gene="gpmI"
                     /locus_tag="AC150V_00910"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR3761514.1"
                     /translation="MPGRSDVPLVALVILDGWGIAQPGPGNAVELAATPVFDELARRF
                     PRTQLDASGPAVGLPAGQMGNSEVGHLTIGSGRILYQDLVRVNHAIEDGSLFENEALV
                     SAFRRARGRGGSVHLLGLVSYGGVHSHIDHLRALLALAEREGMAERVWVHAFTDGRDV
                     SPHAAVSDLADLPAERLATVAGRYYAMDRDRRWDRTRRALAAIRTGAGSVADDAVEAV
                     RASYAAGVTDEFVEPVVLSGRPRIGADDAAIFFNVRPDRARQLSQLLLEEGVDLTTMT
                     RYRDEFDCPVAFAEQEVRETLAEVLSRAGARQLHAAETEKYAHVTYFFNGGVEREWP"
     gene            complement(970..1656)
                     /gene="tpiA"
                     /locus_tag="AC150V_00915"
     CDS             complement(970..1656)
                     /gene="tpiA"
                     /locus_tag="AC150V_00915"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR3761515.1"
                     /translation="MLVAGNWKMFKGPRETRAFLESFKSPDDVDVVVCPPYVSLHDAL
                     RFDVPVYAQNVHWADEGAFTGEISAQMLVELGVTGTIVGHSERRQYFADDDESVRRRA
                     EAALEAGLAVIACVGESEVDREAGATEEVLARQVAPLPRHEALVIAYEPVWAIGTGKT
                     ATPGIAQEAHAFIKSLHQTQVLYGGSVKPENAAELLAGPDVDGALVGGASLDPDSFQA
                     ICQAAATSRS"
     gene            complement(1687..2748)
                     /locus_tag="AC150V_00920"
     CDS             complement(1687..2748)
                     /locus_tag="AC150V_00920"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR3761516.1"
                     /translation="MRLPRSVAGADVRGKRVLVRADLNVPLEDGSVADDTRIRAALPT
                     LRWLLDHDSADVAVCSHLGRPKSDDDRARYAIEPVRARLLELLPDERLQVLENTRFDP
                     GETANDPAAAAALAEGRDLYVNDAFGSAHRAHASTEGVAHLLPAYAGLLLLAELEHLG
                     RLLSDDVGHPFVLITGGAKVGDKLGVLKNLGGRADTVLVGGKMAEELRDENPLDFPVV
                     LPSDVVAAAEFAAGAEARTVSYDAVPEGWLGLDIGPETSGAFAELAAGAGTVFWNGPM
                     GVFEWPRFAEGTNAVARAVGRATDAGAYTVVGGGDSVRAVHEAGLAESVSWISTGGGA
                     SLELLEGKELPGVAAIPAA"
     gene            complement(2783..3778)
                     /gene="gap"
                     /locus_tag="AC150V_00925"
     CDS             complement(2783..3778)
                     /gene="gap"
                     /locus_tag="AC150V_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR3761517.1"
                     /translation="MAVRVGINGLGRIGRNFFRAQRQLQADIDIVGVNDLGDASTMAH
                     LLKYDSVLGPFPGGVELGDGVIRAGGEELKMFSERDPGALPWGELGVDIVLESTGVFT
                     DRAGASKHLEAGAKKVVISAPAKDPDITLVLGVNDGEYDPGKDHIISNASCTTNCVAP
                     LAKVIDDLAGIEEGFMTTIHAYTNDQQILDLPHSDLRRARAAAINLIPTSTGAAKAIG
                     LVLPHLQGKIDGVAVRAPVPTGSLTDLVVRVGREVTTEEVDAAYETAAGGSLDGILQY
                     QPDPIVSTDISGSPYSCIYDSALTMARGSLVKVFGWYDNEWGYSCRLVDLMGRLL"
     gene            complement(4004..>4744)
                     /gene="whiA"
                     /locus_tag="AC150V_00930"
     CDS             complement(4004..>4744)
                     /gene="whiA"
                     /locus_tag="AC150V_00930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR3761518.1"
                     /translation="ALLRGLRVDSEIRTYRQPAFDRSTRFQLHVEGNELALDLLAEAG
                     VLDRDRRPLERPPGHVVARTCCRSAYLRGAFLGSGSLSGPRSPHLELRFPRHAGATFV
                     RVVASTEAVPLRVLDRASHSVAYAKSWDAVQGYLAAAGAVETALSLEERAVVAGVQAQ
                     ANRLANADHANLVRQSEAAQAQLQAIRLLEADGRLAELPDRLRQAAELRSLHPMLSLR
                     ELAARAEPPVTKAGMQRRLAHLVDLAGL"
BASE COUNT          732 a         1650 c         1649 g          713 t
ORIGIN      
        1 ccggccactc ccgctccacg ccgccgttga agaagtaggt cacgtgcgcg tacttctcgg
       61 tctccgcagc gtgcagctga cgcgcgccgg cccgggagag gacttcggcg agagtctccc
      121 gtacctcctg ttctgcaaag gcgaccgggc agtcgaactc gtcccggtaa cgagtcatgg
      181 tcgtcaggtc gacgccctcc tcaaggagta gctgcgacag ctggcgcgcc cgatccggcc
      241 ggacgttgaa gaagatcgcg gcgtcgtcgg ccccgattcg ggggcgcccg ctcagcacga
      301 cgggctcgac gaactcgtcg gtgacgcctg ccgcgtagct cgcccgtacc gcttcgaccg
      361 cgtcgtcggc gacggagcct gcgccggtac ggatcgctgc gagcgcccgc cgcgtgcggt
      421 cccagcggcg atcgcggtcc atcgcgtagt agcgcccggc aacggttgcc aggcgctcgg
      481 ccggaagatc ggcgaggtcg ctcaccgcgg cgtgcggcga aacgtcgcgg ccgtcggtga
      541 acgcgtgcac ccagacccgc tcggccatgc cctcgcgctc ggccagcgca agcagcgcgc
      601 ggagatggtc gatgtgcgag tggacgccgc cgtaggagac gagcccgagc agatgcacgc
      661 ttccgccccg cccgcgggca cgacggaagg cgctcaccag ggcttcgttc tcgaacagag
      721 acccgtcctc gatcgcgtga ttcacccgca cgagatcctg gtagaggatg cgcccggagc
      781 cgattgtcag atgtcctacc tccgagttcc ccatctgtcc ggccggaaga ccgacggcgg
      841 gaccggaggc gtcgagctgt gtgcgcggaa agcgccgggc gagctcgtcg aagacaggcg
      901 tggcggccag ctccactgcg ttgccgggcc cgggttgcgc gataccccag ccgtcgagga
      961 tgacgagcgc tacgagcggg acgtcgctgc ggcctggcaa atcgcttgga acgaatcggg
     1021 atcgagggac gcgccgccga ccagcgcgcc gtcgacgtcg ggcccggcga gcagctcggc
     1081 ggcgttctcg ggcttcaccg agcctccgta cagcacctgg gtctggtgca gggacttgat
     1141 gaaggcgtgt gcctcttggg cgatgccggg cgtcgcagtc ttgccggtac cgatcgccca
     1201 gaccggctcg taggcgatga cgagcgcctc gtgacgaggc agcggcgcaa cctggcgggc
     1261 gagcacctct tccgtggcgc ctgcctcgcg gtcgacctcg ctctccccga cacaggcgat
     1321 gacggcgagg ccggcctcga gggcagcctc agcgcgcctg cggacggatt cgtcgtcgtc
     1381 ggcgaagtac tgacggcgct ccgagtgccc gacgatcgtc cccgtcactc cgagctcgac
     1441 gagcatctgc gcggagatct ccccggtgaa tgcgccctcg tcggcccagt ggacgttctg
     1501 cgcgtacacg ggcacgtcga aacgcagggc gtcgtgcagc gagacgtacg gcgggcagac
     1561 caccacgtcc acgtcgtcgg gcgacttgaa ggactcgagg aaggcacgcg tctcgcgggg
     1621 ccctttgaac atcttccagt tgccggcgac gagcacgcga ggtgatccta caagcgagtc
     1681 cacgcctcac gcggcaggga tggccgccac accggggagc tctttccctt cgagcagctc
     1741 gagcgacgca ccgccgcctg tggagatcca ggagacgctt tctgccaggc cggcttcatg
     1801 gacggcccgc accgagtccc caccgccgac cacggtgtag gcgccggcgt cagtggcccg
     1861 acccaccgcg cgcgcgaccg cgttcgtccc ctcggcgaat cgaggccatt cgaacacgcc
     1921 catcggcccg ttccagaaca cggtgcccgc tcccgcggcc agctctgcga aggctccgct
     1981 cgtctccggc ccgatgtcga gtccgagcca gccctcgggc accgcgtcgt acgaaaccgt
     2041 gcgggcctcg gcgccggccg cgaactcggc ggccgccacc acgtcgctcg gcaggacgac
     2101 ggggaagtcg agcggattct cgtcacgcag ctcctcggcc atcttcccac ccacgagcac
     2161 ggtatcggcc cgcccgccga ggtttttcag cacgccgagc ttgtccccga ccttggcgcc
     2221 gccggtgatc agcacgaaag gatggccgac gtcgtcggag aggagccgac ccaggtgctc
     2281 gagctcggcg aggagtagca gcccggcgta ggcgggcagc aggtgcgcga cgccctctgt
     2341 cgacgcgtgc gcgcgatgcg ccgagccgaa cgcgtcgttg acgtagagat cgcgtccctc
     2401 ggcgagcgcc gcagcggcag cggggtcgtt ggccgtctcg cccgggtcga accgggtgtt
     2461 ctcgagcacc tggaggcgct cgtcgggcag gagctcgagc aggcgagccc ggaccggctc
     2521 gatcgcgtag cgagcgcggt cgtcgtcaga cttgggccgg ccgaggtgcg agcagaccgc
     2581 gacgtcggcc gagtcgtgat cgagcagcca gcgtagtgtg ggaagcgccg ctcggatccg
     2641 cgtgtcgtcg gcgacgctcc cgtcctcgag cggcacgttc agatccgcgc gcacgagcac
     2701 gcgcttgccg cggacgtcgg cgccggcgac cgacctgggc agcctcatcg ggacatccgc
     2761 ttccgcgacg gcacgcggtc gactagagga ggcggcccat cagatcgacg agccggcagc
     2821 tgtaccccca ctcgttgtcg taccagccga agaccttcac cagcgagcca cgcgccatcg
     2881 tcagcgcgct gtcgtagatg cacgagtacg gcgagccgct gatgtcggtc gacacgatcg
     2941 ggtcgggctg gtactggagg atcccgtcga gcgagccgcc cgccgccgtc tcgtaggcgg
     3001 cgtcgacctc ctcggtcgtc acctcgcgtc cgacccgcac cacgagatcc gtcaacgagc
     3061 ctgtgggtac cggggcacga acggcaacac cgtcgatctt tccctgcagg tgcggaagaa
     3121 ccagcccgat cgccttcgcg gctccggtcg acgtagggat caggttgatc gccgcggccc
     3181 tcgcccggcg aaggtcggag tgcggcagat cgaggatctg ctggtcgttc gtgtacgcgt
     3241 ggatcgtcgt catgaacccc tcttcgatcc cggcgagatc gtcgatcacc tttgcgagcg
     3301 gcgcgacgca gttcgtggta cacgaggcgt tggagatgat gtgatccttg cccgggtcgt
     3361 actcgccgtc gttgacaccg agaacgagcg tgatgtcggg atccttcgca ggcgccgaga
     3421 tgacgacctt cttggcgccg gcctcgaggt gcttcgaggc gcccgcccgg tccgtgaaca
     3481 cgcctgtcga ctccaatacg atgtcgacgc cgagctcacc ccagggcagc gcgccgggat
     3541 cccgctcgga gaacattttc agttcctcgc cgccggcgcg aatgacgccg tcgccgagtt
     3601 ccacaccgcc cgggaacggg ccgagcacag agtcgtactt gagcaggtga gccatcgtgc
     3661 tcgcgtcgcc cagatcgttg acgccgacga tgtcgatgtc ggcctggagc tgccgctggg
     3721 cgcggaagaa attgcggccg atcctgccga gtccgttgat tcctacgcgt actgccatcg
     3781 ctgcgctcgc tcctcgtctc gctgtctggg ggtcgccgcg agtcttacca gctttttgat
     3841 taggtgggcc ggggcaggag ctccgaggac gatctcccac ccccacgaag tgcgcgctcc
     3901 agcaccagac ggcagacggg gtctggatcc accgcccggg agcgcgagcc cacctgcttc
     3961 gggagtatga gagacgcgag ccccgtgggc aactgtgggc gcgtcacagg ccagccagat
     4021 cgacgaggtg ggcaagccgt cgctgcatcc ccgccttcgt cacgggcggc tcggcgcggg
     4081 ctgcgagctc gcgcagcgac agcatcggat ggagcgatcg cagctccgcg gcctgcctga
     4141 gcctgtccgg aagctccgcc agccgaccgt ctgcttcgag gaggcgaata gcctggagct
     4201 gggcctgagc cgcctcgctc tggcgcacga ggttcgcgtg gtccgcgttc gccagccggt
     4261 tggcctgcgc ctgaacgcct gccaccacgg cgcgctcctc gagcgacagc gccgtctcga
     4321 ccgcgcccgc cgcggccaga tagccctgga ccgcgtccca gctcttggcg tacgcgacgc
     4381 tgtgcgaggc gcggtcgaga acgcggaggg ggacagcttc ggtcgacgcg accacgcgga
     4441 cgaatgtcgc gcccgcgtgt cgcgggaagc ggagctcgag atgtggtgag cgggggccgg
     4501 agagagaccc gctgccgagg aacgcgccgc gcaggtatgc actgcggcag caggttcgcg
     4561 cgacgacgtg tcccggtggc cgctccagcg ggcgccggtc acgatccaga acgccggcct
     4621 cggcgagcag atcgagtgcg agctcgtttc cttccacgtg tagctgaaag cgcgtcgacc
     4681 ggtcgaaggc gggctgtctg tacgtacgga tctccgagtc gacgcggagt ccacgcagca
     4741 acgc
//
LOCUS       DBNGNK010000034         6553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1939391,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000034 DBNGNK010000000
VERSION     DBNGNK010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6553
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1939391"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="AC150V_00935"
     CDS             <1..725
                     /locus_tag="AC150V_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761519.1"
                     /translation="VNESRELMLTRVVARDQLAAASAVLDDRRESLAGRGVPVRAAVF
                     SSGKPAHDLARFASEHDADLLVLEGGLTSEARDLLEEAACDVALLPEARAVEPPGPDR
                     PILVPFTGAEHDWAGVELAAWLARATNAQLVLAGSATETEDASRLLASASLILQHAVG
                     ISASPLLVEPGAKGLLAAASDASLVVFGLPEDWRRKGIGEVRLAVAQEAAAPTLVVRK
                     GLRPGGLAPAASHTRFTWTIRG"
     gene            complement(715..2598)
                     /locus_tag="AC150V_00940"
     CDS             complement(715..2598)
                     /locus_tag="AC150V_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3761520.1"
                     /translation="MATSVDPRIGSVLVGYRIEELLGRGGMSVVYRAEDLALERKVAL
                     KLLAPELAEQDGFRERFQRESRLAASLDHPGVVPVYEAGEVQGQLYIAMRYVEGTDLG
                     ALLHDEGPMEPAPAIELCAQVADALDEAHEHGLVHRDVKPSNVLLDMRGRCYLSDFGL
                     TKRAGEQTDLTGAGQLMGSSDYIAPEAIEGQSVDGRADVYALGCLLYQCVAGEPPFPR
                     DSELSTLWAHVHDPPPKLAGHPELGAVVAKALAKDREERYPTAGALTQAAQAALPQPE
                     PEHPPRRRRVLVAVAAALVAVAVAAVLAVALTGGDGETSPSTKPTLTPTTDSIQRIDP
                     ETNELVATIAAPVSTGELAVGGGSVWVASREENSVARIDPSENAVVQTIERVGNPTGI
                     AYSAGSVWVVNLRDGIVTQLDPTSLDITGSVSLPPGSGQTYPWGLRASDGAIPLRWAT
                     SDGNAVVIEPRSTDARTIQIGPPTAVVRDIAASESGMLWAALYGARDEFETDLLSFDT
                     SGAKRESFVPLGIPDEACVSVGQSDVWVADFAGEVVFQVDPGGRTVVRQIAVGASPCW
                     MTSGHSSVWVANSGDGTVSRIDAESGELMATIEVGPNPWGIAADENAVWVDVHPTTSS
                     INP"
     gene            2797..3447
                     /locus_tag="AC150V_00945"
     CDS             2797..3447
                     /locus_tag="AC150V_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761521.1"
                     /translation="MTVDRGPGVDLYWLPLGAGGRSVRVNGRVFEAVAAGLERRPSCD
                     LYHCALEVHVADARFVIEMTPIPDGNGGERGVVAQGAVGSRLAERFRIFRYEVRRWRD
                     GVIPDVGEAVESPRRLTGDPLLAQRVLDAVPLVPTFVWGRDESQAGEMWNSNSLISWL
                     IVRGGLSVESVHPPAGGRAPGWRAGLVVARRQEPMADSVGRERAVNPKAPSFRSSR"
     gene            complement(3454..4641)
                     /gene="rhmD"
                     /locus_tag="AC150V_00950"
     CDS             complement(3454..4641)
                     /gene="rhmD"
                     /locus_tag="AC150V_00950"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HIR3761522.1"
                     /translation="MAGFPKITSVRAHVLSGGGADYHDQGAEHWIVGKIATPMSVYPE
                     YRATRSSFGLNALGTVIVEAEAEDGTIGIGTSTGGPPAAWLVEHHLSRFVEGKRADQL
                     ELAWDQMWRASLSYGRKGLPVNAISAIDLAIWDLLGRLRDEPVHQMIGGPVRDEISFY
                     ATGPRPDLAKEMGFIGGKLPLVYAPVEGSEGLRKNVEIAAEMRQRVGDDFFLSYDCWM
                     ALDVEYTLRLIEALRPLKLKWLEEFLPPDDYAGYAEVRRRAPRDILLTTGEHEWTRWG
                     FRQLVDGRCCDIIQPDVNWCGGLTELLKIAAYASAHGVFVVPHGSSVYSYHFVVTRHE
                     SPFAEFLMMAPDAASVVPMFTPLLTGEPVPEQGRIKASSLDRPGFGVELDRSLPLERP
                     FPR"
     gene            complement(4643..5557)
                     /locus_tag="AC150V_00955"
     CDS             complement(4643..5557)
                     /locus_tag="AC150V_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3761523.1"
                     /translation="MRIVQYASGGTTGCGVESGDVVFATGYDDALALIRDGERGLEAA
                     AAAVEVSDPVTVDRILAPLTNPGKIFGSGVNYASHGDEEPGFVFPDEKVIVGFVKFAS
                     AIVGPGDAIVIPPNDDVIKRVPGDTSGPLREHGFAVDYEVEFGVVIGSTAKNVRAEDA
                     LDHVFGYTVINDVGSRSVQFHNMQVDLGKNFDTFCPMGPCLVTKDELPDWENVRIRSI
                     VNGEVRQDALVGEQLGPPPAAIEWLSSVITLEPGDILSTGTPAGCGTFMDPPRFLEPG
                     DTVTVSASGIGELTNPVVAGTTRTYQAG"
     gene            5609..>6553
                     /locus_tag="AC150V_00960"
     CDS             5609..>6553
                     /locus_tag="AC150V_00960"
                     /inference="COORDINATES: protein motif:HMM:NF018682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HIR3761524.1"
                     /translation="MGEGVVDSVVALDDDPTGAQALAGIRVLLEWTPRRVADALSGRH
                     ATHLLTNVRALPADEVEEVVRSAAKAAVGGAPDAHVLLRGDSTLRGHLREELLGLRSV
                     VDPDCALPVLLVPALPAAGRITVGGVHLIERDGARTPLHETEYAGDGVFAYGDAHLLA
                     WAEERSGGLLPAANGRGLPLAELRSKGAVAVAAALEELNAAGRPAALAPDAETDDDLR
                     LIAEGYARAHGLGARALVRCAPAFVGVLSGTTAGGLVDPPPPGGGVLVVCGSYVPTTT
                     RQLARLVAERPDMLIEVDVRALASDDPEVEIERCARALR"
BASE COUNT          934 a         2276 c         2343 g         1000 t
ORIGIN      
        1 cggtcaacga gtcccgcgag ctcatgctga cgcgcgtcgt cgcgcgagac cagctcgcgg
       61 cggcgagcgc cgtcctcgac gatcgccggg aaagcctcgc cggccggggc gttcccgtgc
      121 gggccgcggt gttcagctcc ggcaagcctg cgcacgacct cgcgcgcttc gcctctgagc
      181 acgacgccga cctgctcgtg ctggagggcg gactgacgtc ggaggcccgg gatctgttgg
      241 aggaagcggc ctgcgacgtg gcgctgttgc ccgaggcccg cgcggtcgag ccgccggggc
      301 cggatcgccc aattctcgtc ccgttcaccg gcgccgagca cgactgggcc ggggtcgagc
      361 tggccgcctg gctcgcgcgg gcgacgaatg cccagctcgt tctggcaggc agtgcgaccg
      421 agaccgagga cgcgagccgg ctcctcgcga gcgcgtcgct catcctgcag catgccgtcg
      481 gcatctcggc gtcgccgctc ctcgtcgaac ccggcgccaa gggcctcctg gcggccgcct
      541 cggacgcgtc actggtggtg ttcggcctgc ccgaggactg gcgccgcaag gggatcggcg
      601 aggtgcgcct cgccgtcgcg caggaagctg ctgcgccgac gctcgtcgtc cgcaaaggac
      661 ttcgccccgg cggcctggcg cccgccgcga gtcacacgcg cttcacctgg acgatcaggg
      721 gttgatcgaa gacgtcgtgg ggtggacgtc gacccagacg gcgttctcgt cggcggcgat
      781 gccccacggg ttcgggccga cctcgatcgt ggccatgagc tcgccgcttt cggcgtcgat
      841 tcgcgacacg gtgccgtcgc cgctgttcgc gacccagacc gagctgtggc cggacgtcat
      901 ccagcacggg ctcgcaccga ctgcgatctg gcggacgacc gttcggccgc ccggatcgac
      961 ctgaaacacg acttcgcccg cgaagtcggc cacccagacg tcggactgcc ctaccgacac
     1021 acaggcttcg tccgggatac cgagcggaac gaacgactcc cgcttcgctc ccgaagtgtc
     1081 gaagctgagc aggtcggtct cgaattcgtc tcgggcgccg tagagggcag cccagagcat
     1141 gcccgactcg gacgcggcaa tgtccctcac gaccgcggtc gggggaccga tctgaatggt
     1201 cctcgcgtcg gtgctccgcg gctcgatcac gacggcgttt ccgtccgagg tcgcccagcg
     1261 aagaggtatc gcgccgtccg acgctctgag accccacgga taggtctgcc ccgatccagg
     1321 cggcagcgag acgctaccgg tgatgtcgag cgaggtggga tccaactgcg tgacgatgcc
     1381 gtccctgaga ttcaccaccc aaaccgagcc ggcagagtag gcgatgccgg tcgggttgcc
     1441 gactcgctcg atcgtctgga cgacggcgtt ctcggaggga tcgatccgtg caacgctgtt
     1501 ctcctcgcgg ctcgctaccc aaacggatcc gccgccgacc gccagctctc ctgtcgatac
     1561 cggtgcggcg atcgtcgcga cgagctcgtt cgtctcgggg tcgatgcgct ggatcgagtc
     1621 ggtggtcgga gtgagggtcg gcttggtgct ggggctcgtc tcgccgtcgc cgcccgtgag
     1681 tgcgacggcg aggacggcgg ccacagcgac cgcgacgagg gctgccgcta cggcaacgag
     1741 gacacggcgt cttcgcggcg gatgctcggg ctcgggctgg gggagcgctg cctgagcggc
     1801 ctgggtgagc gcgcccgccg tcggataccg ctcctcccga tccttcgcga gtgccttggc
     1861 gacaacagcg ccgagctcgg ggtggccggc gagcttcggc ggcgggtcgt ggacgtgcgc
     1921 ccagagggtg gagagctcgg agtcgcgcgg gaagggcggc tcgccggcga cgcactgata
     1981 caggaggcag ccgagggcgt agacgtcggc gcggccgtcg accgactggc cctcgatcgc
     2041 ctcgggggcg atgtagtcgc tcgagcccat gagctggccg gcgccggtca ggtcggtctg
     2101 ctcgccggcc cgctttgtga gcccgaagtc gctcagatag cagcggcccc gcatatcgag
     2161 caggacgttg gacggcttga cgtcgcggtg gacgaggccg tgctcgtggg cctcgtcgag
     2221 cgcgtcggcc acttgggcgc aaagctcgat cgccggtgcc ggctccatcg gcccctcgtc
     2281 gtgcaggagc gcgccgaggt cggtgccctc gacgtagcgc atcgcgatgt agagctgccc
     2341 ctgcacctcg ccggcctcgt agacgggcac gacgcccggg tggtcgagcg aggcggccag
     2401 cctcgactcc cgctggaagc gctccctgaa gccgtcctgc tcggccagct cgggcgcgag
     2461 cagcttcagc gccaccttcc gctccaaggc gaggtcttcc gcgcggtaga cgacgctcat
     2521 cccgccgcgg ccgagcagct cctcgatccg gtaccccacc agcaccgagc cgatccgcgg
     2581 atcaacggag gtagccatct gcgcccctcg taacggtaga cacattctcc ctcgtagtcg
     2641 agagcaacag cgggtgccga gagatcgtcc tacggggctg taacagggcc gtaacggacg
     2701 acgagaaagg tgccgacggt aattcgccgc aggcagatcg gtcacggctc cgatgcgagc
     2761 ggactgccat cgcctcacgc tgcagacgat gccgcaatga ctgtggatcg cgggcccggg
     2821 gtcgacctct actggcttcc gctgggagcg ggcggtcgct cggtccgggt gaacgggcgc
     2881 gtcttcgagg ccgtggcagc cggactcgag cggcggcctt cgtgcgacct ctaccactgc
     2941 gcgctcgagg tacacgtagc ggacgccagg ttcgtgatcg agatgacgcc gatcccggac
     3001 ggcaacggcg gggagcgcgg cgtcgtggcc cagggcgccg tcggcagccg cttggcggaa
     3061 cgcttccgga tcttccgcta cgaggtgcgc cgctggcgtg acggcgtgat ccccgatgtc
     3121 ggcgaggcgg tcgagagccc acggcggctc acgggtgacc cgctactcgc gcagcgcgtc
     3181 ctcgacgccg tgcccctcgt cccgacattc gtgtgggggc gcgacgagag ccaggccgga
     3241 gagatgtgga actccaactc gttgatctcc tggctgatcg tccgcggtgg cctctccgtg
     3301 gagtcggtgc atccgcccgc gggagggcgc gctcccggtt ggcgagccgg gctcgtggtg
     3361 gcgcggcgac aggagccgat ggccgactcg gtcgggcgcg agcgcgcggt caacccaaag
     3421 gccccgagct tcaggtcgag ccgctagggc cggtcaccgc gggaacggcc gctcgagcgg
     3481 gagcgagcgg tcgagctcca cgccgaagcc cggacggtcg agagaagacg ccttgatccg
     3541 tccctgctcg ggcacgggct cgccggttag gagcggcgtg aacatcggca cgaccgaggc
     3601 ggcgtcgggg gccatcatca ggaactcggc gaacgggctc tcgtgccggg tgacgacgaa
     3661 gtggtagctg tagacgctcg atccgtgggg gacgacgaag acgccgtgcg ccgaggcgta
     3721 ggccgcgatc ttgagcagct ccgtcagccc gccgcaccag ttgacgtcgg gctggatgat
     3781 gtcgcagcag cggccgtcga ccagctgccg gaagccccag cgtgtccact cgtgctctcc
     3841 cgtggtcagc aggatgtcac gcggcgcgcg ccggcgaacc tccgcatacc cggcgtagtc
     3901 gtccggcggc aggaactcct cgagccactt cagcttgaga ggccgcagcg cctcgatcag
     3961 gcggagcgtg tactcgacgt cgagggccat ccagcagtcg taggagagga agaagtcgtc
     4021 gccgacgcgc tgccgcatct cggcggcgat ctcgacgttc ttgcgcaggc cctcgctgcc
     4081 ttccacgggt gcgtagacga gcggcagctt gccgccgatg aagcccatct ccttggccag
     4141 gtccggccgc ggccccgtcg cgtagaagga gatctcgtcg cgtacgggcc cgccgatcat
     4201 ctggtgcacc ggctcgtccc gcaggcgccc gagcaaatcc cagatggcga ggtcgatcgc
     4261 cgagatcgcg ttgacgggga gccccttgcg gccgtacgag agcgaggcgc gccacatctg
     4321 atcccacgcc agctcgagct ggtcggcccg tttcccctcg acgaagcggg agaggtggtg
     4381 ctccacgagc cacgcggccg ggggcccgcc cgtcgacgtc ccgatgccga tcgtgccgtc
     4441 ctcggcctcc gcctcgacga tcaccgtgcc gagcgcgttc agcccgaacg aggagcgcgt
     4501 cgcgcggtac tcggggtaga cggacatcgg cgtcgcgatc ttgccgacga tccagtgctc
     4561 cgctccctgg tcgtggtagt cggcgccgcc gccgctcagg acgtgcgccc ggacggacgt
     4621 gatcttcggg aagccggcca cctcagcccg cttggtacgt gcgcgtggtg cctgccacga
     4681 ccgggttcgt cagctcgccg atcccgctcg cggataccgt caccgtgtcg cccggctcga
     4741 gaaagcgcgg cggatccatg aacgtgccgc agccggccgg tgttccggtc gagaggatgt
     4801 cacccggctc cagcgtgatg acggagctga gccactcgat cgcggccggc gggggcccga
     4861 gctgctcgcc gacgagcgcg tcctggcgga cctcgccgtt gacgatcgac cggatgcgga
     4921 cgttctccca gtcgggcaac tcgtccttcg tcacgaggca cggtcccatc gggcaaaagg
     4981 tgtcgaagtt cttgccgaga tcgacctgca tgttgtggaa ctgcaccgag cgcgagccga
     5041 cgtcgttgat caccgtgtac ccgaacacat ggtcgagcgc atcctcggct cggacgttct
     5101 tcgccgtcga gccgatgacc acgccgaact ccacctcgta gtcgaccgcg aatccgtgct
     5161 cacggagcgg tcccgacgta tcaccgggca cgcgcttgat gacgtcgtcg ttcggcggga
     5221 tgacgatggc gtcgcccggt ccgacgatcg cgctcgcgaa cttgacgaag cccacgatca
     5281 ccttctcgtc ggggaacacg aacccgggct cctcgtcgcc gtgactcgcg tagttgacgc
     5341 cggagccgaa gatcttcccc gggttcgtca ggggcgcgag gatcctgtcg acggtgacgg
     5401 ggtcggacac ctcgacggcg gccgccgcgg cctcgagccc gcgctcgccg tcgcggatca
     5461 gcgcgagcgc gtcgtcgtac ccggtcgcga agacaacgtc accggactcg actccgcaac
     5521 cggtggtgcc gccgcttgcg tactggacga tgcgcatcgg gcctccacgt ctgtatgaca
     5581 gtacgacgag cctataggct cgcgggaaat gggtgaaggc gtcgtcgact ccgtcgtcgc
     5641 cctcgacgac gaccccacgg gcgcgcaggc tctcgccggc attcgggtgc tcctcgagtg
     5701 gacaccccga cgggtggcgg atgcgctctc gggacggcat gcgacccatc tgctcacgaa
     5761 tgtccgtgcg ctcccggccg acgaggtcga ggaggtcgtg cggtcggccg cgaaggcggc
     5821 tgtcgggggc gctcctgacg cccacgtact gcttcgcggc gactcgacgc tacgcggtca
     5881 cctgcgcgag gagctgctcg ggctgcgctc tgtcgtcgac ccggactgcg ccctgcccgt
     5941 gctcctcgtg cccgcgcttc cggcggcggg ccggatcacg gtcggcggcg tgcatctgat
     6001 cgagcgcgac ggcgcccgca cgcccctgca cgagaccgag tacgccgggg acggagtgtt
     6061 cgcctacggc gacgcgcacc tgctcgcatg ggccgaggag cgaagcggtg ggctgctgcc
     6121 tgctgcgaac gggcgcgggc tgccgctggc agagcttcgc tcgaagggtg cggtcgctgt
     6181 cgcggccgcg ctcgaggagc tgaatgcggc tggccgaccg gcggcgctcg ctccggatgc
     6241 ggagaccgac gacgatctgc ggctgatcgc agagggctac gcgagggctc atggcctggg
     6301 cgcgagggcg ctcgtccgct gcgctcccgc cttcgtgggc gtcctgagcg gcacgaccgc
     6361 aggcgggctc gtcgaccctc cgccgccggg cggcggcgta ctcgtcgtct gcggctccta
     6421 cgtgccgacg acgacccgtc agctcgcgcg tctcgtcgcg gagcggcccg acatgctcat
     6481 cgaggtcgac gtccgcgcgc tcgcctccga cgaccccgag gtggagatcg agcgctgcgc
     6541 gcgtgcactc cgc
//
LOCUS       DBNGNK010000035         3184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_148751,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000035 DBNGNK010000000
VERSION     DBNGNK010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_148751"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="AC150V_00965"
     CDS             complement(<1..803)
                     /locus_tag="AC150V_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3761525.1"
                     /translation="MNANQTTPRGFARAAAPGAPNRRRRLATLPLGLLFLAAGAAAAQ
                     QPPEIVVPLSNPGQPASLEVGLVNGSISVSAHDGSDVVIETTLETDGDGKARSDSRDG
                     LKLIPNTSIGLTVEESSNRVEISGDWSSRATNLVIRVPRLTSLHLSTVNNGDIEVSGA
                     VGDHELSNVNGAITAIDVAGSVVANTTNGDVIVRFDRIEPGKPMSFSTWNGNVDLTLP
                     GDSRAKLRMNAGSGDIYTNFDVVLAPQDVKMSREEGERGFRVRLEKEVVG"
     gene            complement(808..1656)
                     /locus_tag="AC150V_00970"
     CDS             complement(808..1656)
                     /locus_tag="AC150V_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3761526.1"
                     /translation="MNRFPFLVGTLLAASIAAPASADLSESRTLEETFVLGVGGRVVV
                     DTLYGSIRVTGTGGESVRMRVVETIHADDATALERARREVELVTEAREDLVDLYVDGP
                     FRARDRKGWSRHHERHYRVSYDFELEVPAGASLELRTVTDGEIRVTGVHGDFDVSNVN
                     GGIEMRDLRGAGRIHTVNGPITADFSRSPTAATDFKTVNGDVEVAFPPDLAAELELDS
                     RWGELWSEFDVVPLPTEPERRVTKSGKTVIKLGAPLLRVAAGGPRLSFETLNGNVRIR
                     KHDQRR"
     gene            complement(1653..2444)
                     /locus_tag="AC150V_00975"
     CDS             complement(1653..2444)
                     /locus_tag="AC150V_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR3761527.1"
                     /translation="MRCEDLGEGLVDSWDDRLDGAKRAELEEHLANCDGCRDEAEQLE
                     TLWLELGALEPEIEVPSERLRARFYSFLAEEQRRAGRRRWTERLTALWPRRLAPQMAL
                     VAAALVLGIFVGLAGSGWRTGVEIRALRAEIASVSESVGLGLLTHPAASERLRGVGLA
                     GRTAADERIVNALIGLIESDPSVNVRLAAIEALAARVEQPGVGPRLARTLPVQTSPLL
                     QMTLLDVLLPIDGERVLEHARPLLEDETLDAAVRERLLEATGETA"
     gene            complement(2441..3040)
                     /locus_tag="AC150V_00980"
     CDS             complement(2441..3040)
                     /locus_tag="AC150V_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3761528.1"
                     /translation="MRGGPNGDEAESVQRIDEIDDGQVMSSVKAGEVGALGILFERYH
                     VQIYRYCLRMTRNPTVSEDLAQEVFTRLLRYAHTFREGTNFKAWIFRVSRNVCNDHFR
                     RGRREVELEAPDERPAADTPVQQSLERAEELERLGEALDRLPEDRRELLILSRFERRK
                     YSEIAQLLDCSVGAVKVRVHRAMRQLRDIYMDMASEATP"
BASE COUNT          455 a         1113 c         1078 g          538 t
ORIGIN      
        1 ccgacgacct ccttctcgag tcgaacccgg aagccgcgct cgccttcctc gcggctcatc
       61 ttgacgtcct ggggcgccag cacgacatcg aagttggtgt agatgtcgcc cgagccggcg
      121 ttcatccgca gcttggcgcg cgaatcgccc ggcagcgtca ggtccacgtt gccgttccat
      181 gtgctgaagg acatcggctt gccgggctcg atgcgatcga agcgcacgat gacgtcgccg
      241 ttcgtggtgt tggcgacgac cgagcccgcc acgtcgatgg cggtgatggc gccgttgacg
      301 ttcgagagct cgtgatcacc caccgcgccc gagacctcga tgtcgccgtt gttgaccgtc
      361 gagaggtgga gcgaggtgag cctgggaacc cggatgacca gattcgttgc ccggctggac
      421 cagtcgccgg agatctcgac gcggttcgag ctctcctcga ccgtcagccc gatgctggtg
      481 ttgggaatga gcttgaggcc gtcgcgactg tccgagcgcg ccttgccgtc gccgtccgtc
      541 tcgagagtgg tctcgatgac cacgtcgctg ccgtcgtggg cactgacgga gatgcttccg
      601 ttgaccagtc cgacctcgag ggaagccggc tggccgggat tgctgagcgg cacgacgatc
      661 tccgggggtt gctgggccgc cgccgctccg gcggcgagga agagcagacc caggggcagc
      721 gtcgcgaggc ggcgacgccg gttcggcgcc ccgggggcgg ccgcgcgtgc gaagcctcgc
      781 ggggttgtct ggttcgcgtt catctctcta tctcctctga tcgtgcttgc gaatgcgcac
      841 gtttccattc agggtttcga acgacagccg ggggccgccc gcggcgaccc tgagaagcgg
      901 cgctcccagc ttgatgaccg tcttgccgct cttggtgacg cggcgctcgg gctcggtggg
      961 caggggcacc acatcgaact cgctccagag ctcaccccag cgactgtcga gctcgagctc
     1021 ggcggcgaga tcgggtggga aggccacctc gacgtcgccg ttgaccgtct tgaaatcggt
     1081 tgccgccgtc gggctgcggc tgaagtcggc cgtgatcggc ccgttgaccg tgtggatccg
     1141 tccggcgccg cgcaggtcgc gcatctcgat accgccattg acgttcgata cgtcgaagtc
     1201 cccgtggacc ccggtgaccc ggatctcgcc gtcggtcacc gtgcgtagct cgagggacgc
     1261 tccggccggc acctcgagct cgaagtcgta ggagacccga tagtgccgct cgtgatgccg
     1321 cgaccagccc ttgcgatcgc gagctcggaa cgggccgtcg acgtagaggt cgaccaggtc
     1381 ctcgcgggcc tcggtcacca gctccacctc gcggcgggcg cgctccaggg cggtcgcgtc
     1441 gtcggcgtgg atggtctcga cgacacgcat cctgacgctc tctccaccgg tacccgtcac
     1501 ccggatcgag ccgtagagag tgtcgacgac cacccggcca ccgacaccga ggacgaacgt
     1561 ctcttcgagg gtacggctct cgctcaggtc ggccgacgcg ggcgcggcga tcgaggctgc
     1621 caggagggtg ccgaccagga acgggaatcg gttcatgccg tttctcctgt cgcctcgagc
     1681 agacgctcgc gcaccgccgc gtcgagggtt tcgtcctcga gcagcggtcg ggcgtgctcg
     1741 agcacgcgct cgccgtcgat cggcagcagc acgtcgagca aggtcatctg gagcagcggc
     1801 gacgtctgga ccggcagagt acgcgccagc cgcggaccga cgccgggctg ctcgaccctc
     1861 gccgccaggg cttcgatcgc cgcgagcctc acgttgacgc tgggatcgct ctcgatcagc
     1921 ccgatgaggg cgttgacgat gcgctcgtcg gccgccgtgc ggccggcgag accgacgccc
     1981 cgcaggcgct cgctggcggc cggatgggtg agcagcccca gaccgacgct ctcgctgacg
     2041 ctcgcgatct cggcgcgcag ggcgcgaatc tccacccctg tgcgccaacc gctgccagcg
     2101 aggccgacga agatgccgag caccagggcc gcggcgacga gcgccatctg cggcgccagc
     2161 cgtcgcggcc agagcgccgt cagacgctcc gtccaacggc ggcgcccggc gcggcgctgt
     2221 tcttccgcga ggaacgagta gaagcgcgcg cgcaggcgct cgctcggaac ctcgatctcg
     2281 ggctcgaggg cgccgagctc gagccataga gtctccagct gctcggcttc gtcgcgacaa
     2341 ccgtcgcagt tcgccaggtg ctcctcgagc tcggcccgct tggctccgtc cagtcgatcg
     2401 tcccagctgt ccaccagtcc ctctcccagg tcttcgcacc tcatggggtc gcctcgctcg
     2461 ccatgtccat gtagatatct cggagctgcc gcatcgcccg gtggacgcgc acctttacgg
     2521 cgcccaccga gcagtcgagc agctgcgcga tctcgctgta cttgcgccgc tcgaagcggc
     2581 tcaggatgag aagctctcta cggtcctccg gcagccggtc gagcgcctcg ccgagccgtt
     2641 ccagctcctc ggctctctcg agcgattgct ggacgggcgt gtcggcggcc gggcgctcgt
     2701 ccggcgcttc gagctcgacc tcgcgtcggc cgcgccggaa gtgatcgttg cagacgttgc
     2761 ggctgacgcg aaagatccag gccttgaagt tggtcccttc gcggaaggtg tgggcgtagc
     2821 gcagcaggcg ggtgaacacc tcctgcgcca agtcctcgct gaccgtcgga ttcctcgtca
     2881 tgcgcaagca gtagcggtag atctggacgt gatagcgctc gaacaggatg cccagcgcgc
     2941 ccacctctcc ggccttgacc gagctcatca cctgtccgtc gtctatctcg tcgatccgct
     3001 gcacgctctc cgcctcgtcc ccgtttggcc cgcctctcat tgtttgagac catcgccccg
     3061 gggaaaggtt acagcagggg ctgccccgcc ccgagggagc cgccgcaagg gcggccgcag
     3121 accgccgtgg cgcgctttcg caacaccgag gaccaccccc ggcaggggct gggaggcttc
     3181 ctgc
//
LOCUS       DBNGNK010000036         3261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1151758,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000036 DBNGNK010000000
VERSION     DBNGNK010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3261
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1151758"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="AC150V_00985"
     CDS             <1..414
                     /locus_tag="AC150V_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3761529.1"
                     /translation="PDSALGGTYVGNPVAQAAALAVLEVLDEERLVERASLIGERIRT
                     RMVEWQARHPRIGDVRGLGAMLALELVADPDTKEPAPELATAVVEEAANRGLLLLKAG
                     IHGNCIRVLCPLVIADAELDEALGAWEDALAAALG"
     gene            433..1854
                     /locus_tag="AC150V_00990"
     CDS             433..1854
                     /locus_tag="AC150V_00990"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3761530.1"
                     /translation="MALGTMTGELIDGRYEVEAVLGSGGMATVYRARDLELERVVAVK
                     VLDSRWADDPDFVGRFRDEARAAARLTHPNVVGVLDRGAFAEREYIVFEYVDGATLKE
                     LVQQGGPLPASRVAGLGCGVARALAAAHAQGIVHRDVKSQNVVVAEDGRPRVTDFGVA
                     HAPDFEQRTETGAIVGTGAYIAPEQARGERVGSAADVYALGVVLFELVTGAPPYDDVN
                     AVAVAMRHVNDPVPRVLDAQPNCPPDLAALIEQCLAKDASERPAAAAVATQLEHIARH
                     QPQSGWEGQTLVISRRPESRRRRRWPVVALVAGALLAAAVVAAALLWDGGGAVGEGVD
                     VPLEAVASYDPVGGDGERDDTVPLATDGDLDTFWTTEGYGDFEALKPGVGILLDSGGE
                     QQLSTLTVESDLSGFTAEVRMCSSTDCEDATVISQSKLADAATTWDLEATDGRYFLLW
                     ITGLADDVDGRQRAHVNEVTARS"
     gene            1888..>3261
                     /locus_tag="AC150V_00995"
     CDS             1888..>3261
                     /locus_tag="AC150V_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3761531.1"
                     /translation="MAACRSCGADLPDGARFCPSCGTALGPGDSPVPEERKLATVLFA
                     DLVGSTALADGEDPERTRALLERFHAVVTEEIERTGGTVEGFAGDGVMAVFGAPASLE
                     DHAERALHAALAMQDRVARQFEGRLELRVGVNTGDVVVGSERGGSAFVTGDAVNVGAR
                     LEQAAQPGEVLVGERTVAAARGAFEFGEGRVIDAKGKQEGVACHPAVRPLTLARPRGV
                     RGLHRAFVGRDAELELLLATYRRAAAQGEPHLVNVMGDSGVGKSRLMRELWARLERED
                     VPPVLRIGRCLAYGDGITYWPLGEVLKQHLGILEADPPEAVARRLEGRPIMGLALGLN
                     PAESLHPLEAREQLHAGAVAFVEELAADRPAVLLVEDVHWAEPDMLDLVERIVRDARS
                     PVLVLTTARPELLEERPSFGGTRRNATTMWLEPLQPETTARLVSELLQAELPDSVRRL
                     VVERAEGN"
BASE COUNT          467 a         1097 c         1215 g          482 t
ORIGIN      
        1 cccgactcgg cgctcggcgg cacgtacgtg ggcaatccgg tcgcccaggc cgccgcgctt
       61 gccgttttgg aagtgctcga cgaggaacgg ctcgtcgaac gcgcgtccct gatcggtgag
      121 cgaatccgga cgcgcatggt cgagtggcag gctcggcacc cgcgcatcgg ggatgtgcgc
      181 ggtctcggcg cgatgctggc gctcgagctg gtcgcggacc ccgacacgaa ggaacccgca
      241 ccggagctcg ccacggcggt cgtagaggag gccgccaacc gagggctgtt gctgctcaag
      301 gccggcatcc acgggaactg catccgggtg ctctgtccgc tggtgatcgc cgatgcggag
      361 ctcgacgagg cgctcggagc ctgggaggac gcgctcgccg ccgcgctcgg gtagcggacg
      421 ggagcgtagc cggtggcgct cgggacgatg acgggcgagt tgatcgacgg gaggtacgag
      481 gtggaagccg tgctcggctc gggcggcatg gccaccgtgt accgggcgcg cgacctcgag
      541 ttggagcgtg tagtcgcggt gaaggtgctc gactcgcggt gggccgacga tccggacttc
      601 gtcggccgct ttcgcgacga ggcgcgggcc gccgcgcgac tcacgcatcc gaacgtcgtg
      661 ggcgtcctcg accgcggtgc tttcgcggag cgtgagtaca tcgtcttcga atacgtcgac
      721 ggcgccactc tcaaggagct cgtccagcag gggggcccgt tgccggcgag tcgtgttgca
      781 ggactcgggt gcggcgtcgc acgcgcactg gcggcggcgc acgcgcaggg gatagtccac
      841 cgggacgtga agtcgcagaa cgtcgtcgtc gccgaggacg gtcgtccgcg cgtgaccgat
      901 ttcggcgtcg ctcatgcccc cgacttcgag cagcgcaccg aaaccggggc catcgtcggt
      961 acgggggcct acatcgcgcc ggaacaggcg cgcggcgagc gtgtcgggag cgccgcggac
     1021 gtctacgcgc tcggcgttgt cctgttcgag ctcgtcacgg gggcgccccc gtacgacgac
     1081 gtgaatgccg tcgccgtcgc gatgcggcac gtgaacgatc cggtgccgcg cgtcctcgac
     1141 gcgcagccca attgcccacc cgacctcgcc gcactgatcg agcagtgcct cgccaaggac
     1201 gcgtccgagc ggccggcggc agccgcagtc gcgacccagc tcgagcacat cgcccgccac
     1261 cagccgcagt cggggtggga ggggcagacg ctcgtcatct cacggcgccc ggagagccgg
     1321 cgtcgccggc gctggcccgt tgtcgctctc gtcgcgggag ccttgctcgc ggcggccgtc
     1381 gtcgccgccg cactgctctg ggacggcggc ggagcggtcg gcgagggggt cgacgtcccg
     1441 cttgaggcgg tggcgagcta tgaccccgtg ggcggggacg gcgagcgtga cgacaccgtc
     1501 ccactcgcga cggacggcga tctcgacacg ttctggacga cggagggcta cggagacttc
     1561 gaagcgctga aacccggcgt cgggatcctc ctcgacagcg gcggtgagca gcagctctcc
     1621 accctcacgg tggaaagcga cctgtcgggc ttcaccgccg aggttcgcat gtgcagcagc
     1681 acggactgcg aggacgccac tgtcatctcc cagtccaagc tggcggatgc ggcgacgacg
     1741 tgggatctcg aggctaccga cggtcgctac tttctcctct ggatcacggg cctcgcagac
     1801 gacgtcgacg ggcgccagcg cgctcacgtg aacgaagtta cggcccgctc ctgacacggg
     1861 tcgcgcgccg tacgccagaa tcacgtcatg gcggcgtgtc gatcctgcgg cgccgatctc
     1921 ccggacgggg cacgtttctg cccttcctgc ggaacggcgc tgggcccggg tgacagcccc
     1981 gtcccggagg agcgaaagct cgcgaccgtg ttgttcgccg atctggtcgg gtcgacggcg
     2041 ctcgccgacg gtgaggatcc ggagcgaaca cgtgcgctct tggagcgctt ccacgccgtg
     2101 gtgacggagg agatcgagcg aacaggcggc acggtcgagg gcttcgcggg ggacggcgtg
     2161 atggcggtgt tcggcgcgcc cgcctcgctc gaggatcacg ccgagcgcgc gttgcacgcc
     2221 gcgctcgcga tgcaggatcg cgtggcccgt cagttcgagg gtcgcctcga gctgcgcgtc
     2281 ggcgtcaaca ctggggacgt cgtcgtcggc tccgagcgcg gcggatccgc gttcgtcacg
     2341 ggtgacgccg tcaacgtagg cgcacggctg gagcaggccg cgcagccggg cgaggtgctc
     2401 gtgggcgaga gaaccgtcgc agccgcgcgg ggcgcgttcg agttcggcga ggggcgggtg
     2461 atcgatgcga aggggaagca ggaaggcgtc gcctgccacc ccgccgtacg cccactcact
     2521 ctcgctcgcc cacgcggcgt ccgaggactc caccgggctt tcgtcgggcg ggacgctgag
     2581 ctcgagctgc tgcttgcgac gtaccggcgg gcggcggccc agggcgagcc gcatctcgtg
     2641 aacgtcatgg gcgactccgg cgtcgggaag tcgcggttga tgcgtgagct ctgggcacgg
     2701 ctcgagcgtg aggacgtacc gccggtgcta cggatcgggc gttgtcttgc ctacggtgac
     2761 ggaatcacgt actggccgct cggcgaggtg ctcaagcagc acctcgggat cctcgaagcc
     2821 gaccctccgg aggcggtggc ccgtcgtctc gaaggccgtc cgatcatggg gctggccctg
     2881 gggctgaatc ccgccgagag cctgcatcct ctcgaggccc gcgagcagct gcacgccggt
     2941 gcggtggcgt tcgtcgagga gctggccgcc gaccgacccg ccgtgctcct cgtggaggac
     3001 gtccactggg ccgagcccga catgctcgac ctggtcgaac gaatcgtccg cgacgctcgc
     3061 agtcccgtgc tcgtccttac gaccgccagg cccgagctgc tcgaggaacg gccgtccttc
     3121 ggcggcacgc gccgcaatgc cacgacgatg tggctcgagc ccctacaacc ggagacgact
     3181 gcgcgactgg tcagcgagct gctccaggcc gagctgcccg acagcgtgcg acgcctcgtc
     3241 gtcgagcggg cagagggcaa c
//
LOCUS       DBNGNK010000037         3787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1295246,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000037 DBNGNK010000000
VERSION     DBNGNK010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1295246"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1109
                     /locus_tag="AC150V_01000"
     CDS             189..1109
                     /locus_tag="AC150V_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3761532.1"
                     /translation="MSQGCAYINERFVDPEDATISIFDPGFTRGDAVYDTVSVWNGLF
                     FRLDDHVQRFFRSCELCRLACPHDSESVKRILAECVDRAGLDAAYVQAIATRGPFPAG
                     GPRDPRLCENRFIAYAVPYVWIVSPERQSEGIDLAIADARRTPPEAIDPRAKNFNWLD
                     LQRGLFEALDRGADTAVLCTPGGLLAEGPGFNVFLIRDGELLTPRSSVLEGITRQTVF
                     ELAAEIGASSCEADLTPDELRQADEAFICSTAGGIMPVASVDGEPLAGGGPGPLSARL
                     RALYWKKREDRWHGTPVASLLAARSGSPRC"
     gene            complement(1126..2424)
                     /locus_tag="AC150V_01005"
     CDS             complement(1126..2424)
                     /locus_tag="AC150V_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017599957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR3761533.1"
                     /translation="MSNRNDTRLWHPFANMAAVRRSEVVIERGEGVWLWDDAGNRYLD
                     GTAALWYTNVGHGRSEIADAIAAQLKRIEAYSVFGDLATPTTLELADKLAELAPVDDP
                     RIFFGSGGGDGIEAAAKLTRLFWEASGSPERMHIISRANAYHGSQAFGTSIGGIDPNR
                     AGFGPLIPSTSVVAWDSVDALREEIASVGPDRVAAYFMEPVIGAGGVHPPPEGYVEGV
                     TEVCAEHGIVFVLDATICGFGRVGTWFAAERWGVRPDMIVFAKGVTSGYQPLGGVVVS
                     GRVAAPFWERPGKPFRHGQTYAGHATACAAGLANIEIMEREGLVERGSTLEDALYDAL
                     APLEDHPLVAEVRGGTGLMAAVAFDEDVIARHPTLVNDAYVAIRDVGKVMSRPLLGAV
                     AYSPPLTITEDELAELGEGTRAGLDDLLARLEPDLAAAGA"
     gene            2540..>3787
                     /locus_tag="AC150V_01010"
     CDS             2540..>3787
                     /locus_tag="AC150V_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3761534.1"
                     /translation="MALKVAVDVGGTFTDICVLNEETGEIAVAKVPSTPNDPIDAVVE
                     GVQGAAGVDLADVALFSHGTTVATNALITRNLPRAAMVTTKGFRDVIEIRRGTKDDLW
                     DAYEDVAPPYVRRRDRLEVTERVGYDGAVLEPLDEDEARHVADILRRKGVEAVAVCFV
                     NSYANPASEQRMKEILEEELEGVSVSTSSDILPEIFEHERFSTAVANAVLSPIIGSYV
                     GRLQDRLEAGGYDGDVLLLHSGGGVMTPATAERLAVRLASSGIAAGAIACRHIAMLCG
                     FPNSIGLDMGGTSTDISLVYEGQERVTKEWFVEYGYPICFPSIEVLTIGAGGGSLAWI
                     DSAGSLRNGPQSAGANPGPACYGRGNDQATNTDANLVLGRLGGELIGGAMQLDRAAAE
                     KAVGAVGPRIGLDAADAATAIIQV"
BASE COUNT          575 a         1314 c         1345 g          553 t
ORIGIN      
        1 cctcgtaccg gctgcacgcg ccgctcctgg gtgagcacag cgccacgatc ctgcgcgagg
       61 ccggctacac ggacgccgag gtggacacac tggtccagga aggcgtcaaa gctctgccgg
      121 cgcggcgctg aggggccggc gcgccgcaga gtggtagcgt cgccgagtca gcggcgtgag
      181 gaggccgagt gtcccagggc tgtgcctaca tcaacgagcg cttcgtcgac cccgaggacg
      241 cgacgatctc gatcttcgac ccgggcttca cgcgcggcga cgccgtctac gacaccgtgt
      301 cggtctggaa cggcctcttc ttccggctcg acgaccacgt gcagcgcttc ttccgctcct
      361 gtgagctctg tcggctcgcg tgtccgcacg attccgagtc cgtcaagcga attctcgccg
      421 agtgcgtcga ccgggccggg ctcgatgccg cctacgtgca ggcgatcgca acgcgcgggc
      481 ctttccccgc gggcgggccg cgcgacccgc gcctctgcga gaaccgcttc atcgcttacg
      541 cggtgccgta cgtctggatc gtctcgcccg agcggcagag cgagggaatc gacctcgcga
      601 tcgccgatgc ccgccggacg ccgccggagg cgattgaccc tcgcgcgaag aacttcaact
      661 ggctcgacct ccagcgcggc cttttcgagg cgctcgaccg cggcgccgac accgccgtcc
      721 tgtgtacgcc cggcgggctc ctcgccgagg gccccggctt caacgtcttc ctgattcggg
      781 acggcgagct gctgacgccc cgctcgagcg tgctcgaagg catcacgcgg cagaccgtct
      841 tcgagctggc ggccgagatc ggagcgagct catgcgaggc cgatctgacg cccgacgagc
      901 tgcggcaggc ggacgaggct ttcatctgct cgacggccgg cgggatcatg cccgtggcca
      961 gcgtcgacgg cgagccactc gccggcggcg gaccggggcc gctttccgcg cggctacgcg
     1021 ccctctactg gaagaagcgc gaggaccggt ggcacgggac accggtcgcg tccctgctgg
     1081 cggcgcggag cggcagcccc cgatgctgag tcgagcgagc cggggctacg cgccggcggc
     1141 ggcgaggtcg ggctcgagcc gtgcgagcag gtcgtccagc ccggcgcgag ttccctcgcc
     1201 cagctccgcc agctcgtcct cggtgatcgt gagcggcggc gagtatgcga ccgcccccag
     1261 cagcggcctg gacatcacct tgccgacgtc gcggatcgcc acgtacgcgt cgttgaccag
     1321 cgtcgggtgc cgggcaatga cgtcctcgtc gaacgcgacg gcggccatga gtcccgttcc
     1381 ccctcggacc tcggcgacga gcgggtgatc ctccaggggc gcgagcgcgt cgtagagggc
     1441 gtcctcgagc gtgctgcccc gctcgaccag accctcgcgt tccatgatct cgatgttggc
     1501 gaggcctgcg gcacacgcgg tggcgtgccc ggcgtatgtc tgaccgtggc ggaacggctt
     1561 gccgggtcgc tcccagaacg gggcggcgac gcgccccgag acgaccacac cgccgagcgg
     1621 ctggtagccg ctcgtgacgc ccttcgcgaa gacgatcatg tcgggtcgga cgccccagcg
     1681 ctccgcggcg aaccacgtgc cgacacggcc gaagccacag atcgtggcgt ccaggacgaa
     1741 gacgatgccg tgctcggcgc acacctcggt gacgccctcc acgtagccct ccggtggcgg
     1801 atggacgccg ccggctccga tcacgggctc catgaagtac gcggcgaccc gatcggggcc
     1861 gactgaggcg atctcctccc gtagcgcgtc gaccgagtcc cacgcgacga ccgacgtcga
     1921 cgggatcaac gggccgaagc cggcccggtt cgggtcgatg ccgccgatgc tcgttccgaa
     1981 tgcctggctt ccgtggtagg cgttcgcccg gctgatgatg tgcatgcgct cgggcgagcc
     2041 ggatgcctcc cagaagagac gtgtgagctt cgcggccgcc tcgatgccgt cgccgccgcc
     2101 gctgccgaag aagatccgcg gatcgtcgac gggcgcaagc tcggccagct tgtccgcaag
     2161 ctcgagcgtc gtcggcgttg cgaggtctcc gaagacggag tacgcctcga tccgcttcag
     2221 ctgagcggca atcgcatcgg cgatctcgct gcgcccatgg cccacgttcg tgtaccagag
     2281 cgcggctgtg ccgtcgagat agcggttgcc ggcatcgtcc cagagccaca cgccctcgcc
     2341 gcgctcgatc acgacctccg agcgacgcac ggcagccatg ttggcaaatg gatgccagag
     2401 acgggtgtca tttcggttgc tcacgactgg cagtctcgca atccgcgacc gcgaccgcaa
     2461 acggcaggcg cgccgcgtga ggaacgggac gactgctgta ggctggcgga cgcgcggccg
     2521 gcgaccagca aggaggatca tggcactgaa ggttgcggtg gacgttggtg ggacattcac
     2581 cgacatctgc gtcctgaacg aggagacggg cgagatcgcg gttgcgaagg ttccgtccac
     2641 cccgaacgat ccgatcgacg cagtcgtcga gggagttcaa ggagccgcgg gcgtcgatct
     2701 cgcggacgtc gcgctgttct cgcacgggac gaccgtcgcg acgaacgcgc tcatcacgcg
     2761 caacctgccg cgggcggcga tggtgaccac gaagggcttc cgcgacgtga tcgagatccg
     2821 ccggggcacc aaggacgacc tctgggacgc ctacgaggac gtcgcgccgc cctacgtgcg
     2881 gcggcgagac cgcctcgagg tgaccgagcg ggtcggctat gacggcgccg tgctcgagcc
     2941 gctcgacgag gacgaggcgc gccacgtggc ggacatcctg cggcgaaagg gcgtcgaggc
     3001 cgtcgccgtc tgcttcgtca actcgtacgc gaacccggcc agcgagcagc ggatgaagga
     3061 gatcctcgag gaggagctgg agggggtcag cgtctcgacg tcgagcgaca tcctgccgga
     3121 gatcttcgag cacgagcggt tctcgaccgc ggtggcgaac gcggtgctgt cgccgatcat
     3181 cggcagctac gtcgggcgcc tgcaggaccg gctcgaggcg ggcggctacg acggcgacgt
     3241 cttgctgctg cactcgggcg gcggcgtgat gactccggcg accgccgagc gcctggccgt
     3301 gcggcttgcc tcgtcgggca tcgcggccgg cgcgatcgca tgccgccaca tcgcgatgct
     3361 ctgcggcttt ccgaattcga tcgggctcga catgggcggc accagcaccg acatctcgct
     3421 cgtctacgag ggccaggagc gggtgacgaa ggaatggttc gtcgagtacg gctacccgat
     3481 ctgcttcccg tccatcgagg tgctcacgat cggcgcgggc ggcggctcgc tcgcctggat
     3541 cgactcggcg gggtcgctgc ggaacggccc ccagtcggcc ggcgccaacc ccgggccggc
     3601 ctgctacggg cgcggcaacg accaggcgac caacaccgac gcgaacctcg tgctgggacg
     3661 actcggcggg gaactgatcg gcggcgcaat gcagctcgac cgggcggcgg ccgagaaggc
     3721 tgttggagcc gtcggacccc gaattggcct cgatgcggcc gacgcggcca cggcgatcat
     3781 ccaggtc
//
LOCUS       DBNGNK010000038         5360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1653193,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000038 DBNGNK010000000
VERSION     DBNGNK010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1653193"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="AC150V_01015"
     CDS             complement(<1..1081)
                     /locus_tag="AC150V_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761535.1"
                     /translation="MILAAKFLVQLVVRGIGAHAGGGLIPAAGSLLVPTVAFTALALP
                     PSAVQRLTTPVLGESRELIAAIVPLPPVREASPRSPSSTPVNGSSAWLRPEWPRRPIF
                     APLRVALDPAEPQPPAADRPPDPPESRPAAADPMALFVGPTQDAPPPPAEVAADSQPS
                     PATEQVRAGDSPPKPSPPTQREPTTPVDLPAEIEDAPGLDDEPGESVGEPAEPPATQP
                     SEATEPAADNDGLTGDSTATDDPSEAAGDDLIGGAGAEESEPRRQPLPETGPRTEPGS
                     EPELEFDLPELQPEPAQPELQPEPVRQPEPLPEREPELQTDLEPGPQPEPRPESDPEP
                     ETGADIVTNPEAEPGDESHENGGEAD"
     gene            complement(1234..2193)
                     /locus_tag="AC150V_01020"
     CDS             complement(1234..2193)
                     /locus_tag="AC150V_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3761536.1"
                     /translation="MAEIVEVLPGAHGVVAGRRRRLGYAMTLAAAALFAFNGVVSKVI
                     LTSGGIDSQRLTQLRSTGAFVGLVLLLAIVARRTLKITRREIPLLAFYGICGFAFVQW
                     FYFFAIERLPVGIALLIQFTAPVLVALWARFAQRSHVRRRVWAALILSLVGLVLVSQT
                     WSGISLDTAGVIAALGASASLAVYFVVGEHGVGRRDPLSLICFSFLFATVFWAALLPW
                     WSFPFGSLADSTSLLGNLADVTAPVWVLALWMIAMGTIVPFALSIGALRYLPATIVGL
                     FAMAEPPIGATVAYLWLGETLAAVQIVGGLIVLVGIFLAQTSR"
     gene            2227..2904
                     /locus_tag="AC150V_01025"
     CDS             2227..2904
                     /locus_tag="AC150V_01025"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3761537.1"
                     /translation="MRVALFITCLADTLFPAAGKATVGLLERLGVEVEFPREQTCCGQ
                     LHMNSGYPREGGALAERFAQVFAEHELVVSPSGSCVAHVREHVPGPVADRVYELSQFL
                     VDVLGVDDVGSGYEGAVTYHPTCHSLRLLRVGDRPQQLLRAVPALDLLALPAAEECCG
                     FGGTFAVKNAAVSSAMLDEKIAAILSSGADAVCACDSSCLMHIGGGLRRRGADVRPVH
                     LAEILAA"
     gene            2901..4322
                     /locus_tag="AC150V_01030"
     CDS             2901..4322
                     /locus_tag="AC150V_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020015925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HIR3761538.1"
                     /translation="MTQAARDAAPEAFPAAAREGLANSQLRANLAQATSTIRSKRAGV
                     VSELPDWEELRAAGAAIKDATLARLDEVLLELEASVTRAGGTVHWARDAAEANAIVVD
                     LAQAHGVDELVKVKSLATDEIRLNEALQQAGVRPIETDLAELIIQLAGERPSHLLVPA
                     IHKNRTEIRDVFRVALDRPDLSDDPGDLAEAARLYLRAAFLRARFAVSGANFAVAETG
                     TICVLESEGNGRMCTTLPDVLVTVMGIEKAIPTWRDLEVYLQLLPRSSTGERMNPYTS
                     FWTGVRPGDGPRAFHLVLLDAGRTRALADPVGRPALRCIRCSACLNVCPVYRHAGGHA
                     YHTVYAGPIGAILAPQLAGESGSTGSLPFASSLCGACYDVCPVRIDIPSILVHERGKV
                     EAGGLWNLESAGQRQLGRILADRGRYERAQRLARRAQKPAARDGWLKRFPGPLKAWGA
                     TRDLPAVPPETFREWWEARERRT"
     gene            4306..4941
                     /locus_tag="AC150V_01035"
     CDS             4306..4941
                     /locus_tag="AC150V_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR3761539.1"
                     /translation="MSAAREEMLATIRSAVGTERDQVHVPRGYRIGGTSTRDEVVRLF
                     VERVADYRATVEVTADPRAAAARALAEQHARRVLIAPDLPAGLRPRDVELVEDDGLSA
                     DELDGFDGALTTCAFASAETGTIALDGGPGQGRRAITLVPDLHVCLVDARRIVETVPE
                     LVARLAASARDGRPIVLVSGPSATSDIELERVEGVHGPRRLVVLVHDAPGS"
     gene            4970..5043
                     /locus_tag="AC150V_01040"
     tRNA            4970..5043
                     /locus_tag="AC150V_01040"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5004..5006,aa:Pro,seq:tgg)
     gene            5045..>5360
                     /locus_tag="AC150V_01045"
     CDS             5045..>5360
                     /locus_tag="AC150V_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761540.1"
                     /translation="MRRAVAVVTTVAWLLVVSSAIAAAPFQSSVRPIPAPLATRMTGV
                     SWREGGPVHLRDLRLVVARHWGFDGKVRTGRLVVHRDVAREIRSVLERLFDARFPIRR
                     MWL"
BASE COUNT          767 a         1818 c         1967 g          808 t
ORIGIN      
        1 cgtcggcctc gccgccgttc tcatgcgatt cgtcgccggg ctcggcctcg ggattcgtca
       61 cgatgtcggc gcccgtctcg ggctcggggt cggactcggg tcgcggctca ggctggggcc
      121 cgggctcgag atcagtctgc agctccggct cccgctcggg caggggctcc ggctggcgca
      181 cgggctcggg ctgcagctcc ggctgggcgg gctctggctg aagctccggc agatcgaact
      241 cgagctccgg ctccgaccct ggctcggtcc gaggtcctgt ctcgggtagc ggctggcgtc
      301 ttggctcgga ctcttcggcg ccggcgcctc cgatcaggtc atcgcccgcg gcctcggagg
      361 ggtcgtccgt cgccgtgctg tcgcccgtca gtccatcgtt gtcagcggcc ggctccgtcg
      421 cctctgacgg ctgcgtcgca gggggctcgg cgggctcgcc gaccgattcg cctggttcgt
      481 cgtccagccc gggcgcgtcc tcgatctccg ctgggaggtc aacgggcgtg gtcggttccc
      541 tctgcgtcgg cggcgacggt ttcggaggcg agtcgcccgc gcggacctgt tctgtcgctg
      601 gggacggctg gctgtccgcg gccacttccg cgggtggggg cggggcgtcc tgcgtcgggc
      661 cgacgaacaa cgccatcggg tctgcggccg ccggacggga ctcgggggga tcgggtggcc
      721 gatcagcggc cggaggctgc ggctcggcgg ggtcgagcgc gacgcgcaga ggcgcgaaga
      781 tcggtcggcg gggccactcc gggcggagcc acgcggagga gccgttcacc ggcgtcgacg
      841 acggagaccg aggggaggcc tcgcgtaccg gaggcagggg cacgatcgcg gcgatgagct
      901 cccgcgattc gcccaggacg ggtgttgtca ggcgctgcac cgcggagggg gggagggcga
      961 gcgctgtgaa cgcaacggtc gggacgagaa gcgagccggc ggcgggaatc agtccgccgc
     1021 cggcgtgtgc gccgatgccg cggacgacta gctgtacgag gaacttcgcc gccaggatca
     1081 tgctgggagt ccgtctcctc ctcgcgcctt gcgcgtgggt gccgcagggg acagatcgca
     1141 cggtaggacc ggtcgccgcg gtgctcatgc ccctttgcgg ccttctgaat ccctcgttcg
     1201 agggtcgggg cggtcggcgc tgctacgcgg cgctcagcgt gacgtctgtg cgaggaagat
     1261 cccgacgagc acgatcagcc cgcccacgat ctgcacggcg gcgagcgtct cgcccagcca
     1321 gaggtaggcg accgtggccc cgatcggggg ctcggccatc gcgaacaggc ccacgatcgt
     1381 ggccggcagg tagcggagcg ctccgatcga tagcgcgaac ggaacgatcg ttcccatggc
     1441 gatcatccag agcgccagca cccagaccgg agcggtcacg tcggcgaggt tgccgagcaa
     1501 cgaagtcgag tcggcgagcg agccgaaggg gaagctccac cacgggagca gagctgccca
     1561 gaacaccgtc gcaaacagga aggagaagca gatcagcgag agcgggtcac gtctccccac
     1621 cccgtgctcg ccgaccacga agtacacggc cagggaggcg gaggcgccca gggcagcgat
     1681 gacgcccgcg gtgtcgagcg agatgccgct ccatgtctgc gagacgagga cgagacccac
     1741 caatgagagg atgagcgccg cccagacccg ccgcctcacg tgggatcgct gggcgaaacg
     1801 ggcccacaac gcaacgagta caggtgcggt gaactggatc agcagagcga tcccgacggg
     1861 caggcgctcg attgcgaaga agtagaacca ctgcacgaag gcgaaaccgc agatcccgta
     1921 gaaggcgagc agcgggatct cgcgccgcgt gatcttgagc gtgcggcgcg cgacgatcgc
     1981 caacaacagg acgaggccga cgaacgcgcc ggtcgagcgg agctgagtca ggcgttgtga
     2041 gtcgatcccg cccgaagtga ggattacctt cgagaccacc ccgttgaacg cgaacaacgc
     2101 tgcggccgcg agcgtcatcg catatccgag ccgacgtcgc ctgccggcga cgaccccgtg
     2161 cgcgccgggg agaacctcca cgatctcggc caccgcgcga ggctatacgc tcacatcgca
     2221 ccagtcgtgc gagtagcgct cttcatcacc tgtctcgcgg ataccctctt tcccgcagcc
     2281 gggaaggcga cggtcgggct gctcgagcgt ctgggcgtcg aggtcgagtt cccgcgcgag
     2341 cagacgtgct gcgggcagct acacatgaac tccggctacc cgcgcgaggg gggcgctctg
     2401 gccgagcgct tcgcgcaggt gttcgcggag cacgagctcg tcgtttcgcc gtccggctcg
     2461 tgcgtcgcgc acgtgcgcga gcacgtgccg gggccggtgg cggatcgtgt ctacgagctg
     2521 tcccagttcc tcgtggacgt cctcggggtc gacgacgtgg gttccggcta cgaaggcgcg
     2581 gtcacctacc acccgacgtg ccattcgctc cggctcctgc gcgtcggaga ccggccgcag
     2641 cagctgttgc gggccgtgcc ggcgctcgac ctgctcgcgc tccccgcggc cgaggagtgc
     2701 tgcggcttcg gggggacgtt cgcggtcaag aacgcagccg tctcgagcgc gatgctcgac
     2761 gagaagatcg cggcgatcct ctcgtccggt gccgatgccg tgtgcgcctg cgacagctcg
     2821 tgcctcatgc acatcggcgg cgggctgcgc cgacgcggcg cagacgttcg gccggttcac
     2881 ctagccgaga ttctcgcagc atgacgcagg cggcgcgaga cgcggccccg gaggcgttcc
     2941 ccgcagctgc gcgagagggg ctcgcgaaca gccagctgcg ggccaatctc gcgcaggcga
     3001 cctcgacgat tcgttcgaag cgggcgggcg tcgtgtcgga gctgcccgac tgggaggagc
     3061 tgcgggcggc aggggcggcg atcaaggatg cgacgctcgc acgcctcgac gaggttctgc
     3121 tggagctcga ggcgtccgtc acgcgggcgg gaggaaccgt ccactgggcc cgcgacgcgg
     3181 cggaggcgaa cgcgatagtc gtcgacctcg ctcaggccca tggagtcgac gagctcgtca
     3241 aggtgaagtc gctcgccacc gacgagatcc gactcaacga ggcactccaa caggccggcg
     3301 tccggccgat cgagaccgat ctcgcagagc tgatcatcca gctcgccggt gagcggccgt
     3361 cccacctgct cgtcccggcc atccacaaga accgcaccga gattcgggac gtgttccgcg
     3421 tcgcgctcga ccggcccgat ctgagtgacg atccgggcga cctggcggag gccgcgcgcc
     3481 tctacctgcg ggccgccttc ctgcgggccc gttttgcggt atcgggcgcg aacttcgccg
     3541 tcgccgagac agggacgatc tgcgtcctgg agtcggaggg caacgggagg atgtgcacca
     3601 cgctgcccga cgtgctcgtc acggttatgg ggatcgagaa ggcgatcccg acgtggcgcg
     3661 acctcgaggt gtacctccag ctgcttccgc gctcctcgac cggcgagcgc atgaatccct
     3721 atacgtcctt ctggaccggc gtcagaccgg gcgacggtcc gcgtgccttc cacctcgtcc
     3781 tgctcgacgc gggtcgcacc cgggcgctcg cggatcccgt cggccggccg gccctccgct
     3841 gcatcaggtg cagcgcctgc ctcaacgtgt gccccgtgta caggcacgcc ggaggccacg
     3901 cctatcacac ggtctatgcc ggcccgatcg gggccatcct cgcaccgcag cttgcgggcg
     3961 agtcgggctc gacaggctcc ctgccgttcg cgtcgtcgct ctgcggagcc tgctacgacg
     4021 tgtgcccggt tcggatcgac attccttcga tcctggtgca cgagcgcggc aaggtagagg
     4081 ccggcgggct ctggaatctc gaaagtgcgg ggcagagaca gctgggtcgg attcttgccg
     4141 accgcgggcg ctacgagcgt gcccagcggc tggcacgacg ggcgcagaag ccggctgcgc
     4201 gggatggctg gctgaagcgg tttccggggc cgctcaaggc gtggggggcg acgcgggatc
     4261 tccccgccgt tccaccggag acgttccgcg agtggtggga ggcacgtgag cgccgcacgt
     4321 gaagagatgc tcgcgacgat ccggtcggcg gtcggcaccg agcgcgacca ggtgcatgtg
     4381 ccgcgcggct accgcatcgg aggcacgtcg acccgggacg aggtcgtgcg gctcttcgtc
     4441 gagcgtgtcg ccgactaccg cgcaacggtc gaggtgacgg ccgacccgcg cgcggcggcg
     4501 gcgcgggccc tggcggagca gcacgcccgg cgcgtactga tcgcccccga tcttccggcc
     4561 gggctgcgcc cgcgagatgt ggagctggtg gaggacgacg gtctttcggc agacgagctc
     4621 gacggcttcg acggtgccct cacgacctgc gcgttcgcga gcgcagagac cggcacgatc
     4681 gccctcgacg gcggtccggg tcaggggcgg cgcgcgatca cgctcgttcc cgacctccac
     4741 gtctgtctcg tcgacgcccg ccgcatcgtc gagacggtgc cggagctcgt cgctcgcctc
     4801 gcggcgagtg cccgtgatgg ccggccgatc gtgctcgtct ccggtccgtc tgcgacgtcg
     4861 gacatcgagc tcgaacgcgt cgaaggagtc cacggcccgc ggcgcctcgt ggtgctcgtc
     4921 cacgacgcgc cgggctcctg acggcggcgg ccctacactg tcacggcacc ggggtgtagc
     4981 tcagcctggt agagcgcccg ctttgggagc gggaggtcgt cggttcgaat ccggccaccc
     5041 cgatatgagg agagccgtcg ccgtcgtcac gaccgtcgcg tggcttctgg tcgtctcgtc
     5101 cgcgatcgcg gctgctccct tccagagctc cgtcaggccg atccccgcac cgctcgcgac
     5161 gcggatgacc ggtgtctcct ggcgtgaggg cggccccgtg cacctgcgcg acctgcggct
     5221 cgttgtcgcg cgccactggg ggttcgacgg gaaagtgagg acgggtcggc tcgtcgtcca
     5281 tcgggatgtc gcgcgggaga tccggtcggt tctggagcgt cttttcgatg cacggttccc
     5341 gatccgccgg atgtggctga
//
LOCUS       DBNGNK010000039         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1008795,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000039 DBNGNK010000000
VERSION     DBNGNK010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1008795"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="AC150V_01050"
     CDS             complement(<1..828)
                     /locus_tag="AC150V_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR3761541.1"
                     /translation="MEALVAELRERTALVARGGGESAVERHRSRGKLTARERVDRLVD
                     PGSSFLELNALAAWDLYGGDAPSAGIVTGIGVVEGRQCVVVANDATVKGGSYFPLTVK
                     KHLRAQEVAAQNRLPCLYLVDSGGAYLPLQAEVFPDRDHFGRIFYNQARLSAAGIAQI
                     AVVMGSCTAGGAYVPAMSDETVIVRGTGTIFIGGPPLVKAATGQEVSAEELGGADVHT
                     RRSGVADHYATSDEHALAIARQIVRHLGDPEPGPWELAPAEPPAVDAADLYGLVPEDF
                     "
     gene            965..1168
                     /locus_tag="AC150V_01055"
     CDS             965..1168
                     /locus_tag="AC150V_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761542.1"
                     /translation="MGGHEERIRVLRSLGELDLDSEEFGETMLVLAQVLTELDARAAN
                     RQVDLLRGQLRLVPLPDESPRPS"
     gene            1208..2047
                     /gene="ppk2"
                     /locus_tag="AC150V_01060"
     CDS             1208..2047
                     /gene="ppk2"
                     /locus_tag="AC150V_01060"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HIR3761543.1"
                     /translation="MARAAGTDTEIRPTPPRLRKSVYLKELQRLQVELALMQDWCRDT
                     GARIAVVFEGRDASGKGGTIKRIQDRLNPRACRIVALPAPSTREQSQWYFQRYIEHLP
                     SAGEIALFDRSWYNRAGVEKVMGFCTPEEHQRFLRQCPLFERMLVEDGILLRKYWFSV
                     SDKEQERRFKARLDDPRKRWKLSEMDLESRRHYVDFSRAKDEMFVHTDTDYAPWYVVD
                     ADAKRNARINCIAHLLSTIPYEFAEPPPLKLPARQKAEGYVRPDWSLMRHVPDHAESL
                     TAQ"
     gene            2074..2430
                     /locus_tag="AC150V_01065"
     CDS             2074..2430
                     /locus_tag="AC150V_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3761544.1"
                     /translation="MDVHSWNDEPVEWLNPAIGRQLLDTEKMTVARLVLKVGAVVPEH
                     SHEHEQVANVLEGRLRFRIGEEEREVGAGESVVVPSNAPHTVTVVEDAVVLDLFVPAR
                     DDWQRGEDTYLRSPSQ"
BASE COUNT          442 a          910 c          907 g          426 t
ORIGIN      
        1 gaagtcttcg ggcaccaggc cgtagaggtc ggccgcgtcg acggccggcg gctcggcggg
       61 cgcgagctcc cacgggccgg gctccggatc gccgaggtgc cgcacgatct gccgcgcgat
      121 cgccagcgca tgctcgtccg acgtcgcgta gtggtcggcg acgcccgatc ggcgtgtgtg
      181 gacgtccgcg ccgccgagct cctccgcgct cacctcctgc ccggtcgccg ccttcacgag
      241 cggcggcccg ccgatgaaga tcgttcccgt accccgcacg atcacggttt cgtcgctcat
      301 cgcaggcacg taggcaccgc cggcggtgca ggagcccatc acgacagcga tctgcgcgat
      361 ccccgccgcg gacagccgcg cctggttgta gaagatgcgc ccgaagtgat cgcggtctgg
      421 aaacacttcc gcctggagcg ggaggtacgc gccgccggag tcgacgagat agaggcacgg
      481 cagccggttc tgcgccgcca cctcctgtgc tcgcaggtgc ttcttgaccg tgagcggaaa
      541 gtacgagcct cccttcacgg tcgcgtcatt ggcgacgacg acgcactgcc gcccttcgac
      601 cacgccgata cccgtcacga tccctgccga gggcgcgtcg ccgccgtaga gatcccacgc
      661 tgcgagcgcg ttcagctcga ggaagctcga gccgggatcg accaaccgat cgacccgttc
      721 ccgcgcggtc agcttgccgc gcgagcgatg ccgctcgacg gcgctctcgc ctcctcctct
      781 ggcgacgagg gcggtgcgct cacggagctc cgcgaccagt gcttccatcg cggcccggcg
      841 acgctcgaac gtgtcgtcgc ggacggcgtg agatgacagg acgctcacga gggcacgagc
      901 gtagcgcgaa agactggact acccgcctct tgcggccccc gcattgctgt ggaggatgtg
      961 aggcgtgggg gggcacgagg agcgcatcag ggtgctacgc agcctgggcg aactggacct
     1021 cgactcggag gagttcgggg agacgatgct cgtgctggcc caggtcttga ccgagctcga
     1081 cgcgcgcgcc gccaatcgcc aggtcgattt gctccgcggg cagctccgtc tcgtgccgct
     1141 acccgacgag tccccgaggc cttcgtagga cctggtccgc cctgttcggg ccggtacgct
     1201 gcaatcgatg gcacgagcgg cgggtaccga tacggaaatc cggccgacgc cccctcggct
     1261 gcgaaaaagc gtctacctga aggagctcca gcggttgcag gtcgagctgg cgctgatgca
     1321 ggactggtgt cgcgacacgg gcgcccgcat cgcagtcgtc ttcgaggggc gcgatgcatc
     1381 gggcaagggt ggcacgatca agcgaatcca ggaccggctg aacccgcgcg cgtgccgcat
     1441 cgtcgcgctg ccggccccga gcacgcgcga gcagagccag tggtacttcc agcgttacat
     1501 cgagcacctc ccgtcggcgg gcgagatcgc gctcttcgac cgcagctggt acaaccgcgc
     1561 gggcgtcgag aaggtgatgg gcttttgcac ccccgaggag caccagcgtt tcctccgcca
     1621 gtgcccgctg ttcgagcgga tgctcgtgga ggacggaatc cttctccgca agtactggtt
     1681 ctcggtcagc gacaaagagc aggaacgccg cttcaaggcg cgtctggacg acccccgcaa
     1741 gcgctggaag ctctccgaga tggatctcga gtcccgtcgc cactacgtcg acttctcgcg
     1801 ggcgaaggac gagatgttcg tgcacacgga cacggactac gcgccctggt acgtcgtcga
     1861 cgccgatgcg aagcgcaacg cccgcatcaa ctgcatcgcg cacttgctca gcaccatccc
     1921 gtacgagttc gccgagccgc ccccgctgaa gctgccggcc cgacagaagg ccgaaggata
     1981 cgtccggccc gactggagcc tcatgcgcca cgtgcccgat cacgccgagt cactgacggc
     2041 gcagtaggcg ctgccgcgcg tagactggcc gtcatggacg tccattcgtg gaacgacgag
     2101 cccgtcgagt ggctcaatcc ggcgatcggt cgacagttgc tcgacacgga gaagatgacc
     2161 gtggcgaggc ttgtcctgaa agtcggcgcc gtcgtgccgg agcacagcca cgagcacgaa
     2221 caggtggcca acgtgctcga ggggcgcttg cgcttccgca tcggtgagga ggagcgcgag
     2281 gtcggggcgg gagagagcgt cgtcgtcccg tcgaacgcgc cgcacacggt gaccgtggtc
     2341 gaggacgccg tggtgctcga cctcttcgtc ccggcgcgcg acgactggca gcgcggcgaa
     2401 gatacgtacc ttcgttctcc cagtcagtag ggtctagaaa gccctgagag ccgctctctc
     2461 gcggccgagc tccaagacgt ccgatcaccc acgcgcgtgc cgtgtccgga gagcgtgctt
     2521 agcgtggatt cctagacccc aagtgactag ctatccggat gaaggcaagc ccctgtattg
     2581 acgccgttgt tccggatggg atgatgcgcc caatgcgagg aagaaggctc gccgtcgccg
     2641 tcgtgagtct cgtggctttc gttctcgtct ttgccgcctg cggag
//
LOCUS       DBNGNK010000040         2945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1438888,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000040 DBNGNK010000000
VERSION     DBNGNK010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1438888"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1247
                     /locus_tag="AC150V_01070"
     CDS             120..1247
                     /locus_tag="AC150V_01070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR3761545.1"
                     /translation="MTTAVIALAASAAALLIVGAIGIAGGRSRRRSEARITRALDGVA
                     TRMDALSEELSDAITRAQEESRRLRTLGSLGTVVDLDEVLARTVEAAADLPGVDAGLV
                     RVSDSEQMPVVASIGLPVEAAEHHVLTGPPDGGRARLARIAYSYDSDDLRPGAVHVGL
                     VVPLDGEADTVGFLAVFSQDAGWQPDAHVSQDLEAIAEQAGPAIENALRFREARQAAD
                     TDALTGLFNRRAFYETIEREVARAHRYDRRLGLLLLDLDDFKAVNTELGLLVGDSVLA
                     DVADRLRHVARASDVVCRWGGDEFAIILPESTLTDTEGLYARIQAALQRRPPEQVGAI
                     GISAGIAELQHDDDGITFFERAEEALRRAKGDGKGRGIAAS"
     gene            complement(1292..>2945)
                     /locus_tag="AC150V_01075"
     CDS             complement(1292..>2945)
                     /locus_tag="AC150V_01075"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR3761546.1"
                     /translation="ATDGVAEAMRETLFETNPISMMANSGARGSFNQIRQLAGMRGLM
                     ANPKGEIIERPIKANFMEGLSVLEYFISTHGARKGLADTALRTADSGYLTRRLVDVSQ
                     DVIIRDEDCKTKDCVELPVVVDGQLNKSVAGRFAAVAVHKPLKDGKPGKTVLAGKGEE
                     LTLAKIAEIAEALEEHAETFGVPVRSVLKCRSEYGVCRTCYGTFLATGETCEIGDAVG
                     IIAAQSIGEPGTQLTMRTFHTGGVAGADITHGLPRVVEIFEARNPKGAATLAELGGTV
                     EFEDTERGPKITIVSTELDEDGELIPPKEYAFPRRSRLLVRDGETAEPGDALNEGSLN
                     PSDLLMLKGATQTELYLVGEVQKVYKSQGVDIHDKHIELIVRQMLKKVRVESAGDTNL
                     LPGQLVDRVQLERENERVKKEKGEQATYEPLILGITKASLATESFLSAASFQETTKVL
                     TDASIEGKVDHLNGLKENVIIGKLIPAATGLKRYRGIDIEPSEPLPALGLSPETEAEL
                     LSALEEIGEGGAIDLSKLGLSFDDGPASADGDAADGGETETS"
BASE COUNT          435 a         1014 c         1002 g          494 t
ORIGIN      
        1 cctgccaacc agagccggaa tggtagctga aacgccagat ccgggcaata acaggctatg
       61 ccggggcccg gagcgtgagt acagtctgtc tcgaagcatc gcttcggagg ggagagggga
      121 tgacgactgc ggtgatcgca ctcgccgcct cggcggcggc actgctgatc gtgggcgcga
      181 tcggcatcgc cggcgggcgg tcccgaaggc gctccgaagc ccgcatcacg cgggcgctgg
      241 acggcgttgc cacgcggatg gatgcgctct cggaagagct ttccgacgcg atcacccgcg
      301 cgcaggagga gagccggcgg cttcgcacgc tgggatcgct gggaacggtc gtcgacctcg
      361 acgaggtgct ggcccgcacg gtggaggcgg cggccgacct acccggtgtc gatgccgggc
      421 tcgtccgcgt ttccgactct gagcaaatgc cggtcgtggc gtcgatcggc ctaccggtgg
      481 aggcggcaga gcaccacgtt ctcacggggc caccagacgg gggccgtgca cggctggccc
      541 gcatcgccta ctcgtacgac tcggatgatc tgagaccggg agcggtgcat gtcggtctcg
      601 tcgtcccgct cgacggggag gcggacacgg tcggcttcct cgccgtcttc tcacaggacg
      661 cgggctggca gcccgacgcg cacgtgagcc aggatctcga ggcgatcgcc gagcaggcgg
      721 gcccggcgat cgagaacgct ctcagattcc gcgaggcgcg acaggctgcg gacacggacg
      781 ctcttaccgg cctgttcaac cgccgcgcct tctacgagac gatcgagcgg gaggtcgcac
      841 gcgcccaccg ctacgaccgg cgcctgggcc ttctgctctt agacctggac gacttcaagg
      901 ccgtcaacac ggagctcggc ctgctggtcg gcgactcggt cctggcggac gtggccgacc
      961 gcctccggca cgtggcccgc gcctccgacg tcgtctgccg gtggggcggc gacgagttcg
     1021 cgatcatcct gcccgagtcc actctcacgg acaccgaggg cctgtacgcc cgcatccagg
     1081 ccgccctcca gcgacggccg ccggaacagg taggtgcgat cggcatctcc gccggaatcg
     1141 ccgagctcca gcacgacgac gacggaatca cgttcttcga gcgcgccgag gaggcgctgc
     1201 ggcgggcaaa aggcgacggc aaggggcgcg gaatcgccgc cagctgaccc gatccggtga
     1261 gggcggccac gtggccgccc tcaccgtcgg gttacgacgt ctcggtctcg ccgccgtcgg
     1321 cggcgtcgcc gtcggccgag gcgggcccgt cgtcgaacga gagcccgagc ttagacaggt
     1381 cgatcgcgcc tccctcgccg atctcctcca gcgccgagag gagctccgcc tcggtctcgg
     1441 gtgagagacc gagcgcggga agcggctcgg aaggctcgat atcgatcccg cggtaccgct
     1501 tcagtccggt cgccgccggg atcagcttgc caatgatcac gttctccttg agcccgttca
     1561 ggtggtcgac cttgccctcg atcgacgcat cggtcaacac cttcgtggtc tcctggaagg
     1621 aggccgccga gaggaacgac tcggtcgcca gcgatgcctt ggtgatgccg aggatgagcg
     1681 gctcgtacgt tgcctgctcg cccttttcct tcttgacgcg ctcgttctcg cgctcgagct
     1741 gaacgcggtc gaccagctgg cccggcagca ggttcgtgtc gccggcgctc tcgaccctca
     1801 ccttcttcag catctggcgc acgatcagct cgatgtgctt gtcgtggatg tcgacgccct
     1861 gggacttgta gaccttctgc acctcgccca cgaggtacag ctcggtctgc gtcgcgccct
     1921 tgagcatcag caggtcggag gggttcagcg agccctcgtt cagtgcatcg ccgggctccg
     1981 ccgtctcgcc atctcgcacg agcaggcgtg acctgcgggg gaatgcgtac tccttcggcg
     2041 ggatcagctc gccgtcctcg tcgagttcgg tcgagacgat ggtgatcttc gggccgcgct
     2101 ctgtgtcctc gaactcgacc gtgccaccca gctcggcgag cgtggccgcg cccttcgggt
     2161 tgcgggcctc gaagatctcc acgacgcggg gcagaccgtg cgtgatgtcg gcgcccgcga
     2221 cgccaccggt gtggaaggtt cgcatcgtca gctgcgtccc gggctcgccg atcgactggg
     2281 cggcgatgat gccgaccgcg tcaccgatct cgcacgtctc gccggtcgcg aggaacgtgc
     2341 cgtaacacgt ccggcacacg ccgtactcgc tgcggcactt gagcaccgaa cgcaccggga
     2401 cgccgaaggt ctccgcgtgc tcctcgagcg cctcggcgat ctcggcgatc ttcgcgagcg
     2461 tgagctcctc ccccttgccg gccagcaccg tcttcccggg cttgccgtcc ttcaggggct
     2521 tgtgcacggc gacggcggcg aagcgcccgg cgacgctctt gttcagctgc ccgtcgacca
     2581 cgacggggag ctcgacgcag tccttcgtct tgcagtcctc gtcgcggatg atcacgtcct
     2641 gcgacacgtc gaccagacgc cgagtgagat agccggagtc ggccgtccgg agcgcggtgt
     2701 cggcaagacc tttgcgggct ccgtgcgtcg agatgaagta ctcgaggacc gacaggccct
     2761 ccatgaagtt ggccttgatc gggcgctcga tgatctcgcc cttcgggttc gccatcaggc
     2821 cgcgcatacc ggcgagctga cggatctgat tgaaagatcc gcgcgcgccc gagttcgcca
     2881 tcatcgagat cggattcgtc tcgaagagcg tctcgcgcat ggcctcggcg acgccgtcgg
     2941 tcgcc
//
LOCUS       DBNGNK010000041         2961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_221302,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000041 DBNGNK010000000
VERSION     DBNGNK010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_221302"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="AC150V_01080"
     CDS             complement(<1..446)
                     /locus_tag="AC150V_01080"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3761547.1"
                     /translation="MSRALRAVGVETTPERPGPGPVSDQLLRALELSIGRRVEGLLAG
                     DHRSSLLGRGSELAQVRPYIPAVDDVRQIDWKVTARTGEPHVRVQMAERVLVTWLALD
                     TSPSMSFGTAERRKADVALGVALALGHAASARGNRVGLVTFGGAEAT"
     gene            complement(443..1456)
                     /locus_tag="AC150V_01085"
     CDS             complement(443..1456)
                     /locus_tag="AC150V_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3761548.1"
                     /translation="MVSDPPALEGGDEKRERLEEALVEIKRVIAGQDEMLERVLVCLV
                     SGGHLLIEGVPGLAKTLTIKTTAAVLGGTFKRVQFTPDLVPSDLVGTRIYRPGDGDFD
                     TELGPVFCNFLLADEINRAPAKVQSALLEVMQEHQVTIGHDTHHVPEPFLVMATQNPI
                     ESEGTYPLPEAQVDRFMLKVLVDYPERDEELTVVHRSLEDPVEVRQVLELDDLLALQK
                     AALQVYVDPSLISYAVSIATATREPGSFGLTELSEYIAYGASPRGPISLVQSARALAL
                     VHGRDYVLTQDIVALAKDALRHRLVLTYQALAEERSPDDILDAVLAVVPQPQLDLGRT
                     AAA"
     gene            1689..2849
                     /locus_tag="AC150V_01090"
     CDS             1689..2849
                     /locus_tag="AC150V_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018585079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3761549.1"
                     /translation="MDLRDTDEEAAFRGEVRSWIEQNLPEELRSHRGGAARFDGPELR
                     AWSRSLHEAGYAGLTWPQEYGGGGRPHSFQAIFLEESARAEAPPHIGVIGLGMAGPTI
                     IAHGTDEQKARYLANILSADEIWCQGFSEPEVGSDLAAVRTSARLDGDHFVVDGQKAW
                     SSYAHIADYCILITRSDPSSERHEGLTYLIVDMRATGVDVRPLRQITGEAEFNEIFFD
                     GVEVPAANVLGEVGCGWQVAMTTLLHERGTLGFALTATLEVAVQELVSLARDRGATPL
                     QRDAIAREWIELQALRYTNYRSLSSLMKTGIPGPEGSIAKLQWSEANQRLTKLALELL
                     GPDAQLLDENAPYGGYWQHHQLRSRGNTIEAGTSEILRSIVAERVLGLPRSR"
BASE COUNT          484 a          984 c         1058 g          435 t
ORIGIN      
        1 gtcgcttcgg ctcccccgaa ggtcaccagc ccaacccggt ttccccgggc gctggccgcg
       61 tgaccgagcg ccagcgccac gccgagtgcg acgtctgcct tgcgccgctc ggccgtgccg
      121 aacgacatcg agggcgacgt gtcgagtgcc agccaggtga cgagcacccg ctcggccatc
      181 tggacgcgaa cgtgaggctc gcctgtgcgc gcggtgacct tccagtcgat ctggcggacg
      241 tcgtcgacgg cggggatgta cgggcggacc tgcgcgagct cggagcctcg ccccaggagg
      301 gaggagcgat gatcgccggc gaggagaccc tcgacacgac ggccgatcga aagctccagc
      361 gcacggagga gctgatccga gacggggccc ggaccgggac gctccggcgt cgtctcgacg
      421 ccgacagcgc gcagcgcgcg actcatgccg cggcggtgcg cccgaggtcg agctgcggct
      481 ggggtaccac ggcgagcact gcgtcgagga tgtcgtccgg ggaacgctcc tcggcgagag
      541 cctgatacgt gaggacgagc cgatggcgca gcgcgtcctt tgcgagcgcg acgatgtcct
      601 gtgtgaggac gtagtcgcgc ccgtgcacca gggcgagagc tcgcgcgctc tgcacgagcg
      661 agatcggacc gcgcgggctc gcgccgtacg cgatgtactc ggagagctcc gtcagcccga
      721 acgagccggg ctcacgtgta gccgtcgcga tgctcactgc atagctgatg agagacggat
      781 cgacgtacac ctgcagggcc gccttctgta gcgcgagcag gtcgtccagc tcgagcacct
      841 gacgcacttc gacgggatcc tcgagcgagc ggtgaacgac cgtcagctcc tcgtcccgct
      901 ccggatagtc gaccagcacc ttcagcatga aacggtcgac ctgcgcttcc gggagcggat
      961 aggtgccctc ggactcgatc gggttctggg tcgccatcac caagaagggc tcggggacgt
     1021 ggtgggtgtc gtggccgatc gtcacctgat gctcctgcat gacctcgagc agcgccgact
     1081 gcaccttggc gggcgcgcgg ttgatctcgt cggcgaggag gaagttgcag aagaccggcc
     1141 cgagctcggt gtcgaaatcg ccgtcacccg gacggtagat gcgcgtaccg acgaggtccg
     1201 agggcacgag gtcgggggtg aactggacgc gcttgaacgt gccgccgagg acggcggccg
     1261 tcgtcttgat cgtcaacgtc ttggccaggc cggggactcc ctcgatcagc agatggccgc
     1321 cggagacgag gcagacgagc acgcgctcaa gcatctcgtc ctgcccggcg atgacgcgct
     1381 tgatctcgac cagcgcctcc tcgagacgct cccgcttctc gtcgcctccc tcgagcgcgg
     1441 gcggatcgct taccatcgga gcaggcgctt ccggctcggg gacggcctgc ggctcgctgt
     1501 cggtacctga catcggcaac ctccgtgtct cggggccacg agcgggccgc ccgctcgtcc
     1561 ctcgatggta gagagacggc cctgaagggc gggttaaggc cccgtaaaac ggagctgagc
     1621 acagaaatcg cccgcgagct cgccggctag gacgcttgga cgcgacgcgg cggcgttagc
     1681 atcgccccgt ggacctgcgg gacacggacg aggaagccgc ttttcgcggc gaggtacgaa
     1741 gctggatcga gcagaacctc cccgaggagc tgcgcagcca ccgcggtgga gccgcccgat
     1801 tcgacggccc tgagctccgg gcctggagcc gcagcctgca cgaggcgggc tacgccggcc
     1861 tgacgtggcc gcaggagtac ggcggcggtg gacggccgca cagcttccag gcgatctttc
     1921 tcgaggagtc ggcgcgggcc gaggcacctc cgcacatcgg cgtgatcggc ctcgggatgg
     1981 ccggcccgac catcatcgcc cacgggaccg acgagcagaa ggctcggtac ctggcgaaca
     2041 tcctctcggc cgacgagatc tggtgtcagg gtttctccga accggaggtg ggctcggatc
     2101 tcgcggcggt gcgaacgagc gcgcgactcg acggcgacca cttcgtcgtg gacggccaga
     2161 aggcgtggtc ttcctatgcc cacatcgcgg actactgcat cctcatcacc cgtagcgacc
     2221 cgtcctccga gcggcacgag ggcctgacat acctgatcgt cgacatgcgg gcgacaggcg
     2281 tcgacgtgcg accgctgcgc cagatcacgg gcgaggcgga gttcaacgag atcttcttcg
     2341 acggcgtcga ggtgccggcc gcgaacgtcc tgggcgaggt gggctgcggc tggcaggtcg
     2401 caatgacgac gctgctgcac gagcggggaa ccctcggctt cgcgctcacg gcgacgctcg
     2461 aggtggccgt acaggagctc gtttcgctcg cgcgcgaccg cggcgccacg ccgttgcagc
     2521 gcgatgcgat cgcgcgggag tggatcgagc tgcaggcgct gcgctacacg aactaccgct
     2581 cgttgtcgtc gctgatgaag accggcatcc cgggaccgga gggatcgatc gccaagctgc
     2641 agtggtcgga ggcgaatcag cgtctgacca agctcgcgct cgagctgctc ggcccggacg
     2701 cgcaactgtt ggacgagaac gcgccgtacg gcggctactg gcagcatcac cagctccgca
     2761 gccgcgggaa cacgatcgag gcgggaacgt ccgagatcct ccgctccatc gtcgcggagc
     2821 gagtgctcgg gctaccgcgg agccgctgac ggaggcctgc tgatggactt ctcgttcacc
     2881 gccgagcagg acgagcttcg ggcgcaggcg cgttcgtacc tcgagtcgag cggcgagccc
     2941 acgtgggcag agctcgccga g
//
LOCUS       DBNGNK010000042         9610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_795120,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000042 DBNGNK010000000
VERSION     DBNGNK010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9610
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_795120"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1397
                     /gene="guaA"
                     /locus_tag="AC150V_01095"
     CDS             <1..1397
                     /gene="guaA"
                     /locus_tag="AC150V_01095"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR3761550.1"
                     /translation="AVILSGGPASVYAAGAPRVDPGIFGLGVPTLGICSGMQLMAQEL
                     GGQVERTGASEFGKTGLEASDSRLFRDLPPEQVVWMSHRDSVVAPPSGAAVTGSSPST
                     PIAAFEDAERGVYGVQFHTEVVHTPHGQDILKNFLYEVAGAPPAWTPAAVIEEQVERI
                     SAQVGREKVICGLSGGVDSAVAALLVHKAVGDQLTCVFVDHGLLRESEAEQVVETFGG
                     HFHVPLVHVQAQARFLDRLAGVTDPEEKRRIVGEEFIRVFEEEAGRLGDVRFLVQGTL
                     YSDVIESGGADGGMAAKIKSHHNVGGLPADMRMELVEPLRLLFKDEVRRVGEELGLPE
                     RMVWRQPFPGPGLAIRIIGDVTAERLEILRTADAVLQEEIRRAGLYRDLWQSFAVLPA
                     IRSVGVQGDERTYAYPIVIRAVTSEDAMTADWARLPYDVLETISSRIINEIPGVNRVA
                     LDLSSKPPATIEWE"
     gene            1503..2462
                     /locus_tag="AC150V_01100"
     CDS             1503..2462
                     /locus_tag="AC150V_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761551.1"
                     /translation="MRLVHVRLLLVAAAAITASTLAWPAAASEQFSDANVELLDFGVN
                     RKGEALVVYQTPAGLVRRILVWGAVDALPSTDPSVVKQVRFSYDYAGGWGKYRNPKYW
                     RAFYDSCLPYDGPPLPLMVTACKARDGTYWALQSWKRMQPLLGFEPWLPQHTAAELHI
                     SHWTGELPSLEVHTHWTYDGRWQGLFGRFSYLGEPVYGFSSTSRGNPKERYSRNVFID
                     TFNSSYGPGWKRESGILTHAPTGTFCHSFVPQKPFPHYPSQKMRPPAAGERYRVTVKG
                     PGVTPVVRWEGPGLTAADRASASSVKAVFDEMMADDPICLRER"
     gene            complement(2463..2732)
                     /locus_tag="AC150V_01105"
     CDS             complement(2463..2732)
                     /locus_tag="AC150V_01105"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR3761552.1"
                     /translation="MIRRRVRVSGFVQGVWFRESTRQRAESAGVAGWVRNAPDGSVEA
                     VFEGDADAVDRLVDFCRKGPRGAVVETISVEEEPAEGLGGFRVVV"
     gene            complement(2729..3697)
                     /locus_tag="AC150V_01110"
     CDS             complement(2729..3697)
                     /locus_tag="AC150V_01110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761553.1"
                     /translation="MTATVRLRAEDIVVTFGAARALDGATVEVAAGETLALLGPSGSG
                     KSTLLRVIAGLQQADAGSVVMDGRAVDGVAPHRRGIGLMFQDGALFPHRDVGANVAFG
                     LKVTRRPRGERREKTAELLELVGLAGYERRQVDTLSGGEQQRVALARALAPQPRALLL
                     DEPLASLDGPLRDRLREDLGELFNRLDVAVVYVTHDVGEALALGHRIAVLRSGAVVQT
                     AAPDELWRSPAGEWVARFLGMGNVVTRNGRTELVRPEAVAVDAAPRGGNAVVRSSRRS
                     GATTMLHVVLDAGFELDAATLALEPPPPGTRVDVQIDPAGVVELSA"
     gene            complement(3694..5292)
                     /locus_tag="AC150V_01115"
     CDS             complement(3694..5292)
                     /locus_tag="AC150V_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761554.1"
                     /translation="MDGDRAPLSRRPAWIVAAAIPLLFAAVFFVYPLIAIVVRSLEGD
                     GGFSIPFDALVARSTLEIAWFTVWQAAISTLLTLAAGLPIAWALSRFDFRGRRLAQAL
                     VIVPFVLPTVVVASAFVAFLPDRLVQTPVAIILAHVFFNIAVVVRIVGAFWEGLGPGL
                     WDAAATLGASPLRRLREVTLPVLGPAIASATAIVFLFCFTSFGVVVILGGPRYRTLEA
                     AIYDQAVRFFDLRTAAVLALLQLAAVAALLLASGRLEQRLGGPRALAREVRRSPRGGE
                     RLGVAAALIGGAVFIATPLAVLVERSLRTADGYGVAHYRALAEETPALLVEPWRAAVN
                     SLLFALAATMIALVVGLLTAAVAARRSGGSLDTLVMLPLGASAVMLGFGFIIAFDEPP
                     LDLRGSWWIVPIAQALVATPFVVRVLAPSWRAIDDRLRDAATVLGASPRRVFREIDLP
                     LLLRAAGVAAGLAFAVALGEFGATVFVAQGDRPTLPVAIFRFLGRPGALNAGLAASLS
                     VVLMALTATVVLLSQRATTGRRSL"
     gene            complement(5268..6332)
                     /locus_tag="AC150V_01120"
     CDS             complement(5268..6332)
                     /locus_tag="AC150V_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3761555.1"
                     /translation="MKASLCHRLALGVALAAAIAVVAGCGGGEEADAPTEVVLVTHDS
                     FAVSKNVVAAFEAETGLELRILQSGDAGEALNKALLTAGNPQGDVLFGVDDNLLSRAL
                     DEDLFIEYESEERAAIGQEFRTDDLRVTPIDHGEVCLNLDRNWFASRGVEPPGTLDQL
                     TEERYRGLTVVENPATSSPGLAFLLATIERFGEDGWQDYWRDLRANGVRVTDGWEEAY
                     TQQFSGAAGSPGKRPIVVSYATSPAAEVIFAGTPLDESPTVAVEDGCFRQVEYAGILR
                     GAPNEDGARLLIDFLLGERFQADVPGSMFVYPVRDGVTLPAEFDRHAIVPENPLRMEP
                     KRIAEQRDAWIDQWTEIVLR"
     gene            complement(6329..6979)
                     /locus_tag="AC150V_01125"
     CDS             complement(6329..6979)
                     /locus_tag="AC150V_01125"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR3761556.1"
                     /translation="MKQAESVVVVVTGASPAAVREAAAEGRTVIAADGGLEAALAADV
                     DVALLVGDLDSVGQAALDAAERAEIPTERHRPDKDATDLELALDAAVVRSARRVRVLG
                     SDGGRLDHLLGAVLGLAADRYADVELDATLGEATIHVVRSDRTLTGAPGELLTLLAAH
                     GPAEGVVTQGLRFPLRGETLEPGSSRGVSNVFEATQATIALARGVLLAVRPGNEEL"
     regulatory      complement(6968..7081)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7085..7675
                     /locus_tag="AC150V_01130"
     CDS             7085..7675
                     /locus_tag="AC150V_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3761557.1"
                     /translation="MVSHATIRPVETFVGRLAAAQIGTTFNQYRVGPRAPLLRERLSA
                     YLAARHEASLLLVGEAAGYRGCRVSGIPFTSERQLTGSGPSEQTATIVRRMLAELGLE
                     EDVLLWNVVPTHPGTESSNRPPTGAEIEQGARFVESLAAGRRVVAVGRVAERALGAPY
                     VRHPARGGAAAFSAGLRTMLLSSAAGGHESALLSER"
     gene            7672..8100
                     /gene="rplM"
                     /locus_tag="AC150V_01135"
     CDS             7672..8100
                     /gene="rplM"
                     /locus_tag="AC150V_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR3761558.1"
                     /translation="MKTYTAKPGEVQRDWYVVDAEGKTLGRLATRIADTLRGKGKPQY
                     TPHVDTGDFVIVVNAEKIRVTGEKLDKKLVYRHSGYPGGLRTRTLREQLERQPTEVIR
                     KAVKGMLPRNRLAAAQLRKLKIYAGPEPPHAAQAPKLMET"
     gene            8100..8495
                     /gene="rpsI"
                     /locus_tag="AC150V_01140"
     CDS             8100..8495
                     /gene="rpsI"
                     /locus_tag="AC150V_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR3761559.1"
                     /translation="MAVTAQYLGTGKRKTSVARVILRPGDGKTWVNGRSLDDYFPRAT
                     HRTLALSPLKVAGAEGTYDLRVRVHGGGVSGQAGAVRHGVARALVEADPELRVPLKRE
                     GFLTRDARQVERKKAGLHKARKAPQFSKR"
     gene            8562..>9610
                     /locus_tag="AC150V_01145"
     CDS             8562..>9610
                     /locus_tag="AC150V_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR3761560.1"
                     /translation="MARRYFGTDGVRGIVPDDLTLDLVERLGRAATMWAGAGRVLVGR
                     DTRGSGPELEAALARGIASAGGATVLGGVLPTPAVALLAEDLGVVISASHNPPQYNGV
                     KFFLGNGQKLVDAQEEEIEALLDRAGVGGGSVEHASGTEARYIAHVEEAFGSDLSGLR
                     VALDCANGALAGIAPAVFERLGAEVTAIGTAPDGGNINVGCGATDLAQLQRTVVDAGC
                     DLGIAFDGDGDRMLAVDSTGAVVDGDQILAVIALRRRVGLVAVTSMTNLGFHRLMAEH
                     GIRVLTTDIGDRYVLEALRREGGVLGGEQSGHVIWLDGHVTGDGLAAALLLCSCLEGS
                     SLADAASAMPRLAQS"
BASE COUNT         1497 a         3398 c         3364 g         1351 t
ORIGIN      
        1 tcgctgtcat cctctccggc ggaccggcat ccgtctacgc ggccggcgcc ccgcgcgtcg
       61 atcccggcat cttcgggctc ggcgtgccga cgctgggcat ctgctccggc atgcagctga
      121 tggcacagga gctcggcggc caggtggagc gcacgggcgc gtccgagttc ggcaagacgg
      181 gactcgaggc ctccgactcc aggctcttcc gcgatctccc gccagagcag gtcgtgtgga
      241 tgagccaccg cgactccgtc gtcgctcccc caagcggcgc tgccgtcacg ggctcctcgc
      301 cctcgacccc gatcgccgcg ttcgaggacg cggagcgcgg cgtctacggc gtccagttcc
      361 atacggaggt cgtccacacg ccgcacgggc aggacatcct gaagaacttc ctctacgagg
      421 tcgccggcgc gccgcccgcc tggacgccgg cggcggtgat cgaggagcag gtggagcgga
      481 tcagtgccca ggtcggccgc gagaaggtga tctgcggcct ctccggcggc gtcgactcgg
      541 cggtcgcggc gctgctggtc cacaaagcgg tcggcgacca gctcacctgc gtcttcgtcg
      601 accacggcct cctgcgcgag agcgaggccg agcaggtcgt cgagacgttc ggcggccact
      661 tccacgtgcc gctcgtgcac gtgcaggcgc aggcgcgctt tctcgaccgg ctcgcaggcg
      721 tgaccgatcc cgaggagaaa cggcgcatcg tcggcgagga gttcatccgc gtcttcgagg
      781 aggaggccgg ccgcctcggg gacgttcgct tcctcgtcca ggggacgctc tactccgacg
      841 tgatcgagtc cggcggcgcc gacggcggca tggcggcgaa gatcaagtcc catcacaacg
      901 tcggcggcct gcctgccgac atgcgcatgg agctcgtcga gccgctgcgg ctgctgttca
      961 aggacgaggt gcgccgcgtc ggcgaggagc tcgggctgcc ggagcggatg gtgtggcgcc
     1021 agcctttccc ggggccggga ctcgcaatcc ggatcatcgg cgacgtgacc gccgagcggc
     1081 tcgagatctt gcgcaccgcc gacgctgtcc tgcaggagga gatccgccgg gcaggcctct
     1141 accgcgacct gtggcagagc ttcgccgtgc tccccgcgat ccgctcggtc ggcgtccagg
     1201 gcgacgagcg cacatacgcc tacccgatcg tcatccgtgc cgtcacgtcc gaggacgcca
     1261 tgacggccga ttgggcgcgg ctcccctacg acgtcctcga aacgatctcg agccggatca
     1321 tcaatgagat cccgggcgtg aaccgcgtcg cgctcgacct ctcatcgaag ccgccggcga
     1381 cgatcgaatg ggagtagggg acagctagag cccgcctctt gttgtttcgg ccgcgcgaca
     1441 caggcggcga tccctgagtc cagcgatagc gcctcacccc cccgggcccg atccgagagg
     1501 gcatgcggct cgtgcatgtg cgcctgctcc tcgtcgccgc cgcggcgatc acggcctcga
     1561 cactcgcctg gcccgccgcc gcaagcgagc agttctccga cgcgaacgtc gagctgctcg
     1621 acttcggcgt caaccggaag ggcgaggcgc tcgtcgtcta ccagacgccc gcgggcctgg
     1681 tgcgtcggat cctcgtctgg ggcgcagtcg acgcgctccc ctcgaccgac ccgtcggtcg
     1741 tcaagcaggt gcgcttcagc tacgactacg ccggcggctg ggggaagtac cgcaatccga
     1801 agtactggcg cgccttctac gacagctgcc tgccctacga cgggccgccg ctgccgctca
     1861 tggtcacggc ctgcaaggcg cgcgacggca cctactgggc actccagtcg tggaagcgca
     1921 tgcagccgct gctcggcttc gagccctggt tgccgcagca caccgccgcc gagctccaca
     1981 tctcccactg gacgggcgag ctcccctcgc tcgaggtgca cacgcactgg acgtatgacg
     2041 gccgctggca gggcctcttc gggcgcttca gctacctggg cgagccggtg tacggcttct
     2101 cctcgacgag ccgcggcaac ccgaaggagc gctacagccg caacgtcttc atcgacacgt
     2161 tcaactcctc gtacgggccc ggctggaagc gcgagtccgg catcctcacg catgcgccga
     2221 ccggcacctt ctgccacagc ttcgtgccgc agaagccgtt cccgcattat ccgagccaga
     2281 agatgcgacc gccggcagcg ggcgagcgct atcgcgtgac cgtgaagggg cccggcgtca
     2341 ctcccgtcgt ccgctgggaa gggcccggcc tgaccgccgc cgaccgcgcg agcgcctcga
     2401 gcgtgaaggc cgtcttcgac gagatgatgg ccgacgaccc gatctgcctc cgcgagcgct
     2461 gatcagacca cgacgcgaaa gccgccgagc ccctcggccg gctcctcctc gaccgagatc
     2521 gtctcgacga cggccccgcg tggccccttg cggcagaagt ccacgaggcg gtccacggca
     2581 tctgcgtctc cttcgaagac cgcctcgaca gacccgtcgg gcgcgttgcg cacccagccg
     2641 gcgacgcccg ccgactccgc ccgctgccgg gtcgactctc tgaaccacac gccctgcacg
     2701 aagcccgaga cgcggacgcg acggcggatc acgcggacaa ctctacgact ccggccgggt
     2761 cgatctgtac gtcgacccgc gtgccgggtg gcggtggctc gagcgccagc gttgcggcat
     2821 cgagctcgaa tcccgcgtcg agcacgacgt gcagcatggt cgtcgcgccg ctccgacgcg
     2881 agctcctcac gacggcgttg ccacctcgcg gggcagcgtc gaccgccacc gcttcgggtc
     2941 gcacgagctc ggtgcgaccg ttccgagtca cgacgttccc cattcccagg aaccgggcga
     3001 cccactctcc cgccggcgag cgccagagct cgtcgggcgc ggcggtctga accactgctc
     3061 ccgaccgcag cacggcgatc cggtgcccga gtgccaacgc ctcgccgaca tcgtgcgtga
     3121 catagacgac ggcgacgtcc aggcggttga acagctctcc cagatcttcc cgcaaacgat
     3181 cccgcagagg cccgtcgagt gatgccagcg gctcgtcgag tagcagcgcc cgcggctgcg
     3241 gggcgagcgc gcgcgcgagg gcgacccgct gctgctcgcc gcctgagagc gtgtcgacct
     3301 gccgccgctc gtaacccgcg aggccgacga gctcgagcag ctccgcagtc ttctccctcc
     3361 gctccccgcg cggccgcctg gtcaccttca ggccgaaggc gacgttcgcc ccgacgtcgc
     3421 gatgagggaa cagcgcgccg tcctggaaca tgagcccgat tccacgccgg tgcggcgcga
     3481 ccccgtcgac cgcccggccg tccatcacga cgcttcccgc gtcggcctgc tggagtccgg
     3541 cgatcacgcg cagcagagtg gacttgcccg acccgctggg cccgaggaga gcgagcgtct
     3601 cgcccgcggc gacctcgacg gtcgcgccgt caagtgcacg tgccgccccg aacgtgacga
     3661 cgatgtcttc cgcgcgaagc ctcacagtcg ccgtcacagg cttcgccgcc cggtcgtcgc
     3721 ccgctgcgac agcagcacca ccgttgcggt gagcgccatc agcacgacgc tcagcgaggc
     3781 ggccaggccc gcgttcagcg cgcccgggcg cccgaggaaa cggaaaatgg cgacgggaag
     3841 ggtgggtcgg tcaccctggg cgacgaacac ggtcgcgccg aactcgccga gcgcgaccgc
     3901 gaacgcgagg cccgccgcga cgcccgccgc ccgcagcagg agcggcagat cgatctccct
     3961 gaacacgcgc ctcggagacg cgcccaggac ggtcgcggcg tcgcggagcc tgtcgtcgat
     4021 cgcgcgccac gacggggcca gcacgcgtac gacgaacggc gtcgcgacga gggcctgcgc
     4081 gatcgggacg atccaccacg atccccggag gtcgagcggc ggctcgtcga aggcgatgat
     4141 gaagccgaag cccagcatca ctgccgaggc cccgagcgga agcatcacga gcgtgtccag
     4201 cgatccgccg ctgcgcctgg ccgcgacggc ggccgtcagc aggcctacga cgagcgcgat
     4261 catggtggcc gcgagtgcga agaggagaga gttgacggcc gctcgccagg gctcgacgag
     4321 cagcgccggt gtctcctcgg cgagggcgcg gtagtgcgcg acgccgtagc cgtcggcggt
     4381 gcgcagagag cgctcgacca gcacggcgag cggcgtggcg atgaacaccg cgccgccgat
     4441 cagtgcggcg gcgacgccga gtcgctcgcc gccgcgcggg ctgcgccgca cctcgcgggc
     4501 caacgcgcgc gggccgccaa ggcgttgctc gagccgtccg ctcgcaagga ggagcgcggc
     4561 gaccgccgcg agctgcagca gagcgagcac cgcggcggtg cgcaggtcga agaaccgcac
     4621 ggcctggtcg tagatggcgg cctcgagcgt tcggtagcgg ggcccgccca ggatcacgac
     4681 cacgccgaag gaggtgaagc agaagaggaa gacgatcgcc gtcgcggacg cgatcgccgg
     4741 tccgagtacg ggcagcgtca cctcacgtag gcgccgcagt ggtgaggcgc cgagcgttgc
     4801 tgccgcatcc cagagccccg ggccgagccc ctcccagaag gcgccgacga ttcggacgac
     4861 gacggcgata ttgaagaaga cgtgcgcgag gatgatcgcg acaggcgtct gcaccagtcg
     4921 atccggcaag aaggcgacga aggccgaggc gaccacgacc gtcggcagga cgaagggcac
     4981 gatcacgagg gcctgcgcca gccggcggcc gcggaagtcg aaccgggaca gcgcccaggc
     5041 gatggggagt cccgcggcca gcgtcagtag cgtcgagatc gcggcctgcc acaccgtgaa
     5101 ccaggcgatc tcgagtgtcg agcgagcgac gagcgcgtcg aaggggatcg agaagccgcc
     5161 gtcgccctcg aggctgcgaa cgacgatggc gatcagcggg tagacgaaga agaccgccgc
     5221 gaagagcagc ggtatcgccg ccgcgacgat ccacgccggc cgtctgctca gcggagcacg
     5281 atctccgtcc actggtcgat ccaggcgtcc cgctgctcgg cgattcgctt cggctccatc
     5341 cgcagcgggt tttcggggac gatcgcatgt cggtcgaact cggccggcag ggtgacgccg
     5401 tcccgtaccg ggtagacgaa catcgagccc ggcacgtcgg cctggaaacg ctcgccgagc
     5461 agaaagtcga tcagcagccg ggcgccgtcc tcgttgggcg cgccgcggag gatccccgcg
     5521 tactcgacct ggcggaagca tccgtcctcg acggcgacgg tcggcgactc gtcgagcggc
     5581 gtgcctgcga agatcacctc ggcggccggg ctggtcgcgt aggagacgac gatcgggcgc
     5641 ttgcccgggc tgcccgcagc ccccgagaac tgctgcgtat acgcctcctc ccagccgtcg
     5701 gtcacgcgga cgccgttggc gcgcaggtcg cgccagtagt cctgccagcc gtcctccccg
     5761 aagcgctcga tcgtcgcgag caagaacgcg agacccggcg acgacgttgc ggggttctcg
     5821 acgaccgtca gcccgcgata gcgttcctcg gtgagctggt cgagcgtccc gggcggctcg
     5881 actcctcgcg atgcaaacca gttgcggtcg aggttcaggc agacctcgcc gtggtcgatc
     5941 ggcgtcacgc ggagatcgtc ggttcggaac tcctgcccga tcgcagcacg ctcctcggac
     6001 tcgtactcga tgaagaggtc ctcgtcgagc gcgcgcgaga gcaggttgtc gtcgacgccg
     6061 aagaggacgt cgccctgcgg attccctgcg gtgaggagag ccttgttgag agcctcgccg
     6121 gcgtcaccgg actgcaggat ccggagctcg aggccggttt cggcttcgaa cgccgccacg
     6181 acgttcttcg agacggcgaa ggagtcgtgc gtcacgagca cgacctcggt cggagcgtcc
     6241 gcctcttcgc cgccgccgca tccagccacg accgcgatcg cggcggccag cgcgacgccg
     6301 agagccagcc tgtgacacag cgacgccttc acagctcctc gttgcccggg cggacggcca
     6361 gcagcacgcc acgtgcaagt gcgatcgtcg cctgcgtcgc ttcgaagacg ttcgacaccc
     6421 cacggctgga gcccggctcg agcgtctctc cacgcagcgg aaagcggagg ccctgggtga
     6481 caacgccctc ggccggcccg tgtgcggcga gcagggtcag cagctcgccg ggcgcacccg
     6541 tgagagtgcg gtcggatcgg acgacgtgga tcgtcgcctc gccgagcgtg gcgtccagct
     6601 cgacgtcggc gtaacggtca gcggcgagcc cgagcaccgc tccgagcagg tggtcgaggc
     6661 ggccaccgtc gctcccgaga acgcgcactc gccgggccga gcggacgacg gcagcgtcga
     6721 gcgcgagttc gaggtcggtc gcgtccttgt ccggtcggtg ccgctctgtc gggatctcgg
     6781 cccgctcggc ggcgtcgagc gccgcctgac cgacagagtc caggtcgccg acgagcaggg
     6841 cgacgtcgac gtccgccgcg agggccgcct ccagcccgcc gtctgctgcg atcaccgttc
     6901 tgccctccgc ggcggcctcg cgcacggcgg caggggaggc ccccgtcacg accactacta
     6961 cggactccgc ctgcttcatg cgcctccctc cgctggcatt acccagatca ggttcggcgg
     7021 gtcggcgacg cactcgatcg ccctctcagc ccggctgccc cgagctcccc gatatgactt
     7081 atccatggta tcgcatgcga cgattcgccc cgtggagact ttcgtggggc gcctggccgc
     7141 cgcgcagatc ggaacgacct tcaatcagta ccgtgtcggc ccgcgcgcgc cgctgctgcg
     7201 cgagcgactc tccgcgtacc tcgcggccag acacgaggcg tcgctcctgc tggtcgggga
     7261 ggcggccgga tatcgaggct gccgggtgtc gggcatcccg ttcacatcgg agcgacagct
     7321 caccggttcg ggcccgagcg aacagacggc gacgatcgtg cgacggatgc tcgccgagct
     7381 ggggctcgag gaggacgttc tcctgtggaa cgtcgtcccg acccatccgg gaacagagag
     7441 cagcaatcgg cccccgacgg gtgccgagat cgaacaggga gcccgatttg tcgagtcgct
     7501 cgccgcaggg cgccgggtgg tggccgtcgg acgtgttgcc gagcgcgcgc tcggagctcc
     7561 gtatgttcgc cacccggctc ggggcggagc ggcggcgttc agcgccgggc tgcggacgat
     7621 gctgctatca tccgccgccg gcggacacga gtccgctctt ctttctgaga gatgaagacc
     7681 tatacggcca agccgggaga agtgcagcgc gactggtacg tcgtcgacgc cgaggggaag
     7741 accctgggtc gactggcgac gcgcatcgcc gacacgttgc ggggaaaggg caagccgcag
     7801 tacacgccgc acgtcgacac cggcgacttc gtgatcgtcg tcaacgccga gaagattcgc
     7861 gtcacgggtg agaagctcga caagaagctc gtctaccggc actcgggcta cccgggcggg
     7921 ctccggactc gcaccttgcg tgagcagctc gagcgtcagc cgaccgaggt gattcggaag
     7981 gcggtcaaag gtatgttgcc gaggaaccgc ctcgccgcgg cccagctgcg gaagctgaag
     8041 atctatgccg gaccggagcc cccgcacgcc gcgcaggcac cgaaactgat ggagacgtga
     8101 tggcagtaac cgcccagtac ctcggaacag gcaagcgcaa gacgtcggtc gcccgggtga
     8161 tcctgcggcc gggcgacggc aagacgtggg tcaacggccg ttcgctcgac gactatttcc
     8221 cgcgcgccac gcaccggacg ctcgcgttgt cgccgctgaa ggtcgcgggc gccgagggca
     8281 cctacgacct gcgggtgcgg gtgcacgggg gcggcgtcag cggtcaagcg ggtgcggtcc
     8341 gtcacggtgt cgcccgggcg ctcgtcgagg cggatcctga gctgcgggtt ccgctcaagc
     8401 gtgagggctt cctcacacgt gacgcgcggc aggttgagcg caagaaggcc ggtctgcaca
     8461 aggcacgcaa ggcaccgcag ttctccaagc gctgacgccc tggccgacgc cgcagtggcg
     8521 tcagagcctc gcatgtagcc tggtcgaacc cttttcgagc catggcgcgt aggtacttcg
     8581 gcacggacgg cgttcggggg atcgttccgg acgacctgac tctcgacctc gtcgagcgtc
     8641 tgggccgcgc cgccacgatg tgggcgggtg ctggccgtgt cctcgtgggg cgtgacacgc
     8701 gcggctcggg cccggagctc gaagctgcgc tggcgcgcgg gatcgcgtcg gcaggcggcg
     8761 cgaccgtgct cggcggcgtg ctgccgacgc cggcggtggc gctccttgcc gaggatctcg
     8821 gcgtcgtgat ctccgcgtca cacaacccgc ctcagtacaa cggcgtcaag ttctttctcg
     8881 gcaacgggca gaagctggtg gacgcgcagg aagaggagat cgaagctctg ctcgaccgtg
     8941 cgggagtcgg cggagggtcc gtggagcatg caagcggcac cgaggcgcgg tacatcgcgc
     9001 atgtcgaaga ggccttcggc tcggacctca gtgggctgcg cgttgcgctc gattgcgcca
     9061 acggcgcttt ggctggaatc gcaccggccg tcttcgagcg gctcggtgcc gaggtcaccg
     9121 cgatcgggac cgcgccggac ggcggcaaca tcaacgtcgg ttgcggagca acggatcttg
     9181 ctcagctcca gcgcacggtc gtcgacgcgg gctgcgacct cggcatcgcg ttcgacggtg
     9241 acggcgaccg catgctggcc gtcgactcga ccggcgccgt agtcgacggt gaccagatcc
     9301 ttgccgtaat cgcccttcgc cggcgcgtcg ggctcgtggc ggtcacctcg atgacgaatt
     9361 tgggcttcca tcgcctgatg gcggagcacg gaattcgcgt gctcacgacg gacatcggcg
     9421 accgctacgt gctcgaggcg ctgcgcagag agggaggcgt gctgggcggc gagcagtcgg
     9481 gccatgtgat ctggctggac gggcacgtca ccggcgacgg gctggccgcg gcgctgcttc
     9541 tctgctcgtg cctggaagga tccagcctcg ccgacgcggc gagtgcaatg ccccgcctcg
     9601 cgcagagcaa
//
LOCUS       DBNGNK010000043         5936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_78832,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000043 DBNGNK010000000
VERSION     DBNGNK010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_78832"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC150V_01150"
     CDS             complement(<1..202)
                     /locus_tag="AC150V_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761561.1"
                     /translation="MSNVGIFAWGIVVSLLVFTAAGLMVYGAILDGRAQKNFQDGNAG
                     PDSLDGPPELVPTGVATDRDTAK"
     gene            complement(199..375)
                     /locus_tag="AC150V_01155"
     CDS             complement(199..375)
                     /locus_tag="AC150V_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008865203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761562.1"
                     /translation="MNNLLVPRALIALAAFISFVLSISLWFSGSESEGIFVGLWVPSI
                     LALGAFLLAGRRLR"
     gene            complement(502..1758)
                     /gene="lysA"
                     /locus_tag="AC150V_01160"
     CDS             complement(502..1758)
                     /gene="lysA"
                     /locus_tag="AC150V_01160"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR3761563.1"
                     /translation="MLDLFPDSARLSGATVHLGGVAASELADRFGTPLVVYCEQTLRA
                     RARALRAALGDGRLFYGTKAFPNVELLRLLREEGVGADVASEGELAFAEAAGLSGDEL
                     VVHGNNKGERLFSEAARIGAVVVLDAPDEAELAAAAGVERAHVRVTLGVDADTHEAIR
                     TGHHGSKFGLPPDQAEVLVADALARGVELLGLHVHVGSQLADFDAQAETIVQLASFAA
                     RCRRRLDWTPRVADLGGGFGIRHYPEEEVADAAELATSAAETACTAFAEAGLPEPEIW
                     LEPGRALVGRAGVTLYRVGAVKHLPERIWVTVDGGMSDNPRPQLYDARYTALSALRAD
                     EAADVTVSVAGMHCESGDVLIDDVDLPAPRRGDLLAVPATGAYTLAMASTYNGVPRPA
                     AVFVGDGQARLIRRRETVADLFALEL"
     gene            1903..4341
                     /gene="leuS"
                     /locus_tag="AC150V_01165"
     CDS             1903..4341
                     /gene="leuS"
                     /locus_tag="AC150V_01165"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR3761564.1"
                     /translation="MERYDPKAIEGKWQQVWEDEQAFVVSNPDLDDPARGAQKSYVVE
                     MLPYPSGELHMGHALNYTIGDVVTHVRRRKGMQVLRPMGYDAFGLPAENAAIREGGHP
                     RVVTERNIAAIRDQMRRMGWAIDWSREISTAEPGYYRWTQWLFLRFYERGLAYRKEAP
                     VKWCPVDQTVLANEQVIDGHCERCGTEVEAKNLTQWFFRITQYADELLDEMDLLEQWP
                     DRVLTMQRNWIGRSQGAEVLFRVDGSGEELPVFTTRPDTLFGATFFVLAPEHPLVLEL
                     VSGSEHEPEVLDYVRHAAARSEVEREEKEKDGVFTGRYAVNPVNDERIPIWVADYVLM
                     GYGTGAIMAVPAHDERDHAFAERYGIEIRTVVRPAEGDAPEEGAFSAHTEDEVLLDSG
                     EFTNQSSPEAKENIVAWLAERGLGTSKIGYRLRDWLLSRQRYWGCPIPIVHCPECGDV
                     PVPDDDLPVLLPEVDDYVPKGRSPLAAAEGWVTTSCPRCGGAGERETDTMDTFVDSSW
                     YFIRYCDPRNDGGPFDRRIADYWMPVNQYIGGVEHAILHLMYARFFTKVMQELGLVSC
                     REPFARLFNQGMITSGGAKMAKSKGNVINPLSYADRYGADAVRTYTLFMGPADEDMDW
                     QDNGLEGVWRFLNRLWRVALEQSAKLASQEPGDGSLTRKTHRTIAKVSDDIDRRYAFN
                     TPIAAVMELVNEIQRAPDDPGARFAAETAVSLIQPYAPHIAEELWQRLGRERLWETPW
                     PVPDRALLVEDEIEVVVQVNGKVRDRLRVPADIAEDDLIALAHRSERVQAHFNGGEPK
                     KTIVVPGKLVNFVI"
     gene            4405..4995
                     /locus_tag="AC150V_01170"
     CDS             4405..4995
                     /locus_tag="AC150V_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR3761565.1"
                     /translation="MDNVPVISLTRRHMLLVALVLLALLALLGKRLAQSGATEPPRPA
                     AAIKPVAAATQHRLVVHVVGSVRQPGLYRLAEGARVADAVRRAGGATRAAELAGVNLA
                     APIVDGAQIVVPRRAGRGAAGTVTHAGPAVPAKVSLAVASVEQLDELPGVGPITAAKI
                     VDHRTANGPFRAVDDLDAVPGIGPATIERLREHVTP"
     gene            4992..>5936
                     /locus_tag="AC150V_01175"
     CDS             4992..>5936
                     /locus_tag="AC150V_01175"
                     /inference="COORDINATES: protein motif:HMM:NF024956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3761566.1"
                     /translation="MIRRALELGWPTALVAAACVGLMAANWIRPGAGLLLLVGAAATA
                     VGFCAAGAAARAGALAAVLACAGLWWGGLRLEALDESALLPRVGETRDARVVVTGPAR
                     SARFSLRVPAEVREFAGETMRERVLLELPPGRAPPQGSVLALRARPVEPRGPDSGFDE
                     RGWLARKGVHVVVRGESYRVLGRRGGVGKLADRLRAHVSGALAGGSDGERTAVLAGVV
                     LGEDEGIDESLREDFRASGLYHLLAVSGQNVAFVVGGTLAACWLLGIGRLVGEAAAIA
                     AVAAYVLAVGWQPSVVRAAVAGVLASLAWIVARPRDRWH"
BASE COUNT          924 a         1980 c         2105 g          927 t
ORIGIN      
        1 gttttgccgt gtcgcgatcc gtggcgaccc cggtgggcac gagctcagga ggcccgtcga
       61 gagaatcggg gccggcgttg ccgtcctgga agttcttttg tgcacggccg tcgaggatcg
      121 cgccgtagac catgagcccg gctgctgtga agaccaggag tgagacgacg atgccccagg
      181 cgaagatgcc gacgttgctc atcgcagccg ccttccggcc aggaggaacg caccgagagc
      241 gaggatcgaa ggcacccaga gcccgacgaa gatgccctcc gactcgctgc cggagaacca
      301 gagcgagatg gagagcacga acgaaatgaa ggccgccagg gcgatcagtg cgcggggaac
      361 gagaaggttg ttcattgttg tgttttcctc tccgtccata ttttgtatag atactgtaga
      421 gagtaggagg tcgtttgtca actttttgtc gaagaacggt acagagcgac cctgaagccg
      481 aggattcgtg ccggctggtg cctagagctc gagtgcgaac aggtcggcca ccgtctcacg
      541 gcgccggatc aaacgcgcct gcccgtcgcc tacgaagaca gcggcgggcc ggggcacccc
      601 gttgtacgtc gacgccatcg ccagcgtgta cgcgcccgtc gcgggaacgg caagaagatc
      661 accgcgtcgt ggcgccggca ggtcgacgtc gtcgatgaga acgtcgcccg actcgcagtg
      721 catacccgcg acgcttacgg tgacgtccgc ggcctcgtcc gcgcgcaggg ccgaaagggc
      781 ggtgtagcgg gcgtcgtaga gctgtggccg gggattgtcg gacatgccgc cgtcgacggt
      841 tacccagatc cgttccggca gatgcttcac cgcgccgact cggtacagcg tgactcccgc
      901 acggccgacg agcgcgcggc cgggctcgag ccagatctcc ggttccggca gacctgcctc
      961 cgcaaacgcc gtgcatgcgg tctcggccgc cgacgttgcg agctccgccg cgtcggcaac
     1021 ctcctcctcg gggtaatggc ggataccgaa tccgccgccc agatctgcga cgcggggcgt
     1081 ccagtcgagc ctgcgccgac agcgggcggc aaacgaggcg agctgcacga tcgtctccgc
     1141 ctgggcgtcg aagtcggcca gctgggagcc gacgtgcacg tgaagcccga gcagctcgac
     1201 gccgcgtgcg agtgcgtctg ccacgagcac ctcggcctga tcgggcggca gaccgaactt
     1261 cgaaccgtgg tgaccggtgc ggatcgcctc gtgcgtgtcc gcgtcgactc cgagcgtcac
     1321 ccggacgtgg gcgcgttcga cgccggcggc ggcagcgagc tctgcctcgt cgggcgcgtc
     1381 gagtaccaca acggcaccga tccgcgcagc ttccgagaag agtctctctc ccttgttgtt
     1441 gccgtggacg acgagctcgt cacccgagag ccccgcggcc tcggcaaagg cgagctcacc
     1501 ctccgaggcg acgtctgcgc cgacgccctc ttcccggagc aggcgaagca gctcgacatt
     1561 cggaaaggct ttcgtgccgt agaacagacg gccgtcgccg agcgccgcgc gtagtgcccg
     1621 cgcgcgtgcc cgcaacgtct gctcgcagta gacgacgagc ggcgtgccga accgatcggc
     1681 gagttctgac gcggcgacgc cgccgaggtg aaccgtcgct ccactgaggc gggccgagtc
     1741 gggaaacagg tcgagcattc gccgattctg cctgttacgc tgtcttcgca tggagcagct
     1801 tcttccgtag cgcagggaac ctccgcggca gccgccgcgc cgtttccccc gtggcctctc
     1861 gaacgagagg cttttttctt tccgcgaaca atttgaggag agatggagcg gtacgacccg
     1921 aaggcgatcg agggcaagtg gcagcaggtc tgggaggacg agcaggcgtt cgtcgtctcc
     1981 aatccggatc tggacgaccc ggcccggggt gcccagaaga gctacgtggt cgagatgctc
     2041 ccgtacccgt ccggcgagct gcacatgggg catgcgctca actacacgat cggggacgtc
     2101 gtcacccacg tgcgccgtcg gaaaggcatg caggtcctgc ggcccatggg ctacgacgcg
     2161 ttcggcctcc cggccgagaa cgccgcgatc cgcgaaggcg gtcatccgcg ggtcgtcaca
     2221 gagcggaaca tcgccgcaat ccgcgatcag atgcgccgga tgggctgggc gatcgactgg
     2281 tcgcgcgaga tctccacggc cgagccgggc tactaccgct ggacgcagtg gctattcctg
     2341 cgcttctacg agcggggact ggcgtaccgg aaggaagccc ccgtcaagtg gtgcccggtc
     2401 gaccagaccg tccttgccaa cgagcaggtg atcgacggcc actgtgagcg gtgcgggacg
     2461 gaggtcgagg ccaagaactt gacgcagtgg ttcttccgca tcacgcagta cgcggacgag
     2521 ctgctcgacg agatggatct gctggagcaa tggcccgacc gagtgctgac gatgcagcgc
     2581 aactggatcg gccgctcgca gggtgccgaa gtgctgttcc gtgtcgacgg cagcggtgag
     2641 gagctcccgg tcttcacgac ccgccccgac acgctgttcg gggccacctt cttcgtcctc
     2701 gcaccggagc atccgctcgt tctcgagctc gtctcgggat ccgagcacga gccggaggtg
     2761 ctcgactacg ttcgtcatgc cgcggcccgc tccgaggtgg agcgcgagga gaaggagaag
     2821 gacggtgtct tcaccggccg ctacgcggtg aaccccgtca acgacgagcg gatcccgatc
     2881 tgggtcgccg actacgttct gatgggttac ggaacaggcg ccatcatggc ggtgcctgcg
     2941 cacgacgagc gcgaccacgc cttcgccgag cggtacggga tcgagatccg cacggtcgtg
     3001 cgtccggccg agggcgatgc gccggaggag ggcgcgttct ccgcgcacac cgaggacgag
     3061 gtgctgctcg actcgggtga gttcaccaat cagtcgagcc ccgaggcgaa ggagaacatc
     3121 gtcgcctggc tcgccgagcg ggggctgggt acgtcgaaga tcggctatcg actgcgcgac
     3181 tggctgctct cccgacagcg ctactggggc tgccccatcc cgatcgtgca ttgcccggag
     3241 tgcggcgatg tgcctgtgcc cgacgatgac cttccggtgc tcctgcccga ggtggacgac
     3301 tacgtgccga aggggcgctc gccactcgcc gccgccgagg gctgggtcac gacgagctgt
     3361 ccgcgctgcg ggggcgcggg agagcgcgag acggacacga tggacacgtt cgtcgactcg
     3421 tcctggtact tcatccgcta ctgcgatccg cgtaacgacg ggggcccgtt cgaccgtcgc
     3481 atcgccgact actggatgcc tgtcaaccag tacatcggcg gagtcgagca cgcaatcctc
     3541 cacttgatgt acgcgcgctt cttcacgaag gtcatgcagg agctcggcct cgtctcgtgc
     3601 cgcgagccgt tcgcgcggct cttcaatcag gggatgatca cgagcggcgg cgccaagatg
     3661 gccaagtcga aggggaacgt gatcaatcca ctctcctacg ctgaccgata cggggccgac
     3721 gcggtgcgga cgtacacgct cttcatggga ccggcagacg aggacatgga ttggcaggac
     3781 aatggtctcg agggcgtctg gcgcttcctc aaccggctct ggcgggtcgc gctcgagcag
     3841 tccgccaagc tggcgagcca ggagccggga gacgggtcgc tgacccgcaa gacgcaccgg
     3901 acgatcgcca aggtgagcga cgacatcgac cgccgttacg cgttcaacac gccgatcgcg
     3961 gccgtgatgg agctggtcaa cgagatccag cgggcgcccg acgatccggg cgcgcgcttc
     4021 gcggccgaga cggccgtctc cctgatccag ccctacgcac cgcacatcgc cgaggagctc
     4081 tggcagcggc tggggcgcga gcggctgtgg gagacgccct ggccggtgcc cgatcgggcg
     4141 ttgctggtgg aggacgagat cgaggtcgtc gtgcaggtga acgggaaggt gcgcgaccgc
     4201 ctccgcgtgc ccgcggacat cgccgaggac gacctgatcg ccctcgcgca ccgctcggag
     4261 cgcgtgcaag cgcacttcaa cggcggtgag cccaagaaga cgatcgtcgt gcccggcaag
     4321 ctcgtgaact tcgtcatcta gccgcgcggc cggcgctctt tcggcacgag ctagctgcag
     4381 agacgcgtct gttttgtcgt caaaatggac aacgtgccgg tcatctccct cacacgacgg
     4441 cacatgctcc tggtcgcgct cgtcctgctg gcgctccttg cgctgctggg caagcgactt
     4501 gcccaatcgg gcgcgaccga gcctccccgc ccggccgccg cgatcaaacc ggtcgcggcg
     4561 gccacccagc accggctcgt cgtccacgtc gtcggatccg tacggcaacc gggcctctac
     4621 cgactggccg aaggcgcgcg tgttgccgac gcggttcgtc gcgcgggcgg cgcgactcgg
     4681 gccgccgagc tggcgggcgt taacctcgcc gcgccgatcg tggacggcgc gcagatcgtc
     4741 gtcccgcggc gtgccggccg cggtgccgcc gggacggtca cgcacgccgg gccggccgtc
     4801 cccgcgaagg tcagtctcgc agtcgcgagc gtcgagcagc tcgacgagct cccgggcgtg
     4861 ggcccgatca cggccgcgaa gatcgtcgac catcggacgg ctaacggccc gtttcgagcg
     4921 gtggacgacc tcgacgccgt cccgggcatc ggaccggcca cgatcgagcg gttgcgagag
     4981 cacgtcaccc cgtgatccgg cgcgcgctgg agctgggatg gccgacggcg ctcgttgccg
     5041 ccgcctgcgt cggcctcatg gccgcgaact ggattcggcc gggcgccggg ctgctcctcc
     5101 tggtaggcgc cgcggcgact gcagtcggct tctgcgccgc cggtgcggct gcgcgggcgg
     5161 gggcgctcgc agcggtgctc gcgtgcgcag ggctctggtg gggaggactt cgcctcgagg
     5221 cgctggatga gagcgcgctc cttccgcgtg tgggcgagac gcgcgacgcc cgcgtcgtcg
     5281 tcaccggtcc ggcccggtcg gcgaggttct cgctgcgtgt gccggccgag gtcagagagt
     5341 tcgccggcga gacgatgcgc gagcgcgttc tgctggagct gccgccggga cgggcgccgc
     5401 cccaagggtc cgtgctcgcg cttcgagcgc gtccggtgga accgagaggg cccgacagcg
     5461 ggttcgacga gcgcggttgg ctcgcgcgaa agggcgtaca cgtcgtggtg cgtggcgagt
     5521 cgtaccgcgt gctcggccgg cgcggcggcg tcggcaaact cgccgaccgg ctgcgagcgc
     5581 acgtctccgg cgcgctcgct ggcgggagcg acggcgagag aaccgctgtg ctcgccggcg
     5641 tcgtcctcgg tgaggacgag ggcatcgacg agagcctgcg ggaggacttc cgcgcgtcgg
     5701 gtctctatca cctactcgcc gtatccggac agaacgtggc gttcgtcgtc ggaggcacgc
     5761 tcgccgcgtg ttggctcctc ggcatcggtc ggctcgtcgg cgaggctgcc gcgatcgcgg
     5821 cggtcgcggc ctatgtcctc gccgtcggct ggcagccgtc cgtcgtccgc gctgcggtgg
     5881 cgggcgtcct cgcctcgctc gcctggatcg tcgcgcggcc gcgcgatcgc tggcac
//
LOCUS       DBNGNK010000044         3305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_150775,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000044 DBNGNK010000000
VERSION     DBNGNK010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_150775"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1190
                     /locus_tag="AC150V_01180"
     CDS             201..1190
                     /locus_tag="AC150V_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3761567.1"
                     /translation="MHSALAIEVSGLEKSFGETRAVRGIDLAVSRGKVYGMLGPNGAG
                     KTTTIRILATLLRPDGGEAKILGHDIVHEADAVRAAISLTGQLASVDEDLTGEENLVL
                     IGRLLGFRHRQAKARAAELLEAFELSEAGGRLVKGYSGGMRRRLDLAASIIVTPELMF
                     LDEPTTGLDPRSRNQVWDIIRALAAGGTTILLSTQYLDEADQLADGIAVIDHGRVIAE
                     GTPGQLKASVGGSALLVRLLDPNDRPAAERVLARVLGADVYFESDPAALSAPCADADR
                     AADVVGELARSGIGIASFSLGQPTLDEVFLALTGHPAEEDGAAGTAEDGAGEA"
     gene            1187..2029
                     /locus_tag="AC150V_01185"
     CDS             1187..2029
                     /locus_tag="AC150V_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761568.1"
                     /translation="MSTAPARTSRQQSHEEALRKALSSTERPARAGAPAAVLAFGWRG
                     MLKIKHVPEQLIDVTLTPVLLVLMFTYLFGGAIAGSTSEYLQFVLPGVLVISVLFTTV
                     YSGVTLNTDVTKGVIDRFRSLPIWRPSPLIGAVLGDSVRYVLGAAVIIALGAALGYRP
                     EGGATGVVAAVALVVVFAFGLSWVFTTVGLLMRAPNAVTNAGFMALFPLTFLTNVFVE
                     PETLPAGLETFVNINPISHVVTAARALMDGGFAGLETAVALGTAALLTAVFAPLTSYL
                     YRRK"
     gene            complement(2043..2372)
                     /locus_tag="AC150V_01190"
     CDS             complement(2043..2372)
                     /locus_tag="AC150V_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR3761569.1"
                     /translation="MGKFMLLYKGPATPVEEMKPEQVQAVMKEWEAWMGRIGAALTDV
                     GAPMANGEAVVDDGSAGSTSQLNGYSIVEAEDLVAAKALVDGHPFLSEGSGKFSVEIH
                     ELMPIPM"
     gene            complement(2432..2761)
                     /locus_tag="AC150V_01195"
     CDS             complement(2432..2761)
                     /locus_tag="AC150V_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761570.1"
                     /translation="MIEGRRALLINVIVTYKVKADRVAENETLVRAVYDELREIADPD
                     VHYATFKKEDGRTFVHIAFFPSAEKQKVLSESQAFRAFQADLPDRCEVPPQPEPVAPV
                     DSYNFSL"
     gene            complement(2940..3218)
                     /locus_tag="AC150V_01200"
     CDS             complement(2940..3218)
                     /locus_tag="AC150V_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761571.1"
                     /translation="MYASVRSYDDPSLAAALAARADEIRNVIRGVPGFRSYYLVAAGD
                     GTVSVTVCDDQAGAEESNRVAADWLRSTMPELGGSPPAVSAGPVVLDA"
BASE COUNT          493 a         1155 c         1113 g          544 t
ORIGIN      
        1 aacgctcggg cggttcagct cggcctccct ctcgacggac acgaccgcga tgccgtcgac
       61 gccgcgctcg acgaggcacg cgaccggttc ggcggcaacg cggtcacccg ggcagtgctc
      121 ctcggcagcg accgcggatg gtcgatgccg ttgctcccgg actgagtcga acgtgaggta
      181 gggcgtatag cctgggcccg atgcactcgg ctctggcgat cgaggtgtcg ggcctcgaga
      241 agtccttcgg cgagacgcgg gccgtgcgcg gcatcgacct agcggtcagc cgcggcaagg
      301 tctacggcat gttggggccg aacggcgccg gcaagacgac gacgatccgg atcctggcca
      361 ccctgctgcg ccctgacggg ggcgaggcga agatactcgg ccacgacatc gtccacgagg
      421 ccgacgccgt gcgcgccgcg atcagcctga ctgggcagct cgcttcggtc gacgaggatc
      481 tgaccggcga ggagaacctc gtcttgatcg gtcggctcct gggcttccgg caccggcagg
      541 cgaaggcgcg cgctgcggag ctcctcgagg cgttcgagct ctccgaggcc ggcggtcggc
      601 tcgtgaaggg ctactcgggc gggatgcgac gccggctcga ccttgccgcc agcatcatcg
      661 tcacgccgga gctcatgttc ctcgacgagc cgacgaccgg tttggatccg cgttcccgca
      721 accaggtctg ggacatcatc cgtgcactcg cggcaggcgg caccactatc ctgctctcga
      781 cgcagtacct ggacgaggcc gaccagcttg ccgacgggat cgccgtgatc gaccacggcc
      841 gcgtgatcgc cgagggcact cccggccagc tgaaggcctc cgtcggaggg tcggcgctgc
      901 tggttcgctt gctcgacccg aacgaccgac cagccgccga gcgggtgctc gcgcgggtgc
      961 tcggcgccga cgtctacttc gagtcggacc cggccgcgct gtccgcgccg tgcgccgacg
     1021 ccgaccgcgc cgccgacgtg gtcggtgagc ttgcccggtc gggcatcggc atcgccagct
     1081 tctcgctcgg ccagccgacc ctggacgagg tgttcctggc cctgacgggc cacccggccg
     1141 aggaggacgg cgcagcagga acggccgagg acggagcggg cgaagcatga gcactgcccc
     1201 cgctcgaacc tctcggcagc aatcgcacga ggaggcactg cgcaaggcgc tctcgtcgac
     1261 cgagcgtccc gcccgcgcgg gtgcgccggc cgcggtgctc gcgtttggct ggcgcgggat
     1321 gctcaagatc aagcacgtac cggagcagct catcgacgtg acgctcaccc cggtgctcct
     1381 cgtcctcatg ttcacgtact tgttcggcgg cgcgatcgcc ggctcgacga gcgaatacct
     1441 ccagttcgtc ctccccggcg tgctcgtgat ctcggtgctg ttcaccaccg tctattccgg
     1501 cgtcacgctg aacaccgacg tcaccaaggg ggttatcgac cgctttcgca gcctgccgat
     1561 ctggcggccg tcgccgctga tcggagccgt gctcggcgac agcgtccgct acgtcctcgg
     1621 cgcggcggtc atcatcgcgc tcggcgcggc gctcgggtac cggcccgagg gaggcgctac
     1681 gggcgtcgtc gcggcagtgg cgctcgtggt cgtcttcgct ttcggcctct cgtgggtctt
     1741 caccaccgtc ggactgctga tgcgggcccc gaacgcggtc acgaatgcgg gtttcatggc
     1801 gctgttcccg ctcacgttcc tcaccaacgt gttcgtcgag cccgagacgc tcccggcggg
     1861 gctggagacg ttcgtcaaca tcaacccgat ctcgcacgtc gtcaccgccg cgcgggcgct
     1921 catggacggc gggttcgcag ggctcgagac ggccgtcgct ctcgggacgg ccgcactgct
     1981 caccgcggtg ttcgcaccgc tgacgagcta cctgtaccgg cgcaagtagc ctccgccgga
     2041 gctcacatcg ggatcggcat cagctcgtgg atctcgaccg agaacttgcc gctgccctcg
     2101 ctgaggaacg ggtggccgtc gacaagcgct ttcgcggcga ccaggtcctc ggcttcgacg
     2161 atcgagtagc cgttgagctg agaagtcgag ccggccgacc cgtcgtcgac gactgcctcg
     2221 ccgttcgcca tcggagcgcc gacgtcggtc agagcagcac cgatcctgcc catccacgcc
     2281 tcccactcct tcatcacggc ttgtacctgc tccggcttca tctcctccac gggagtcgcc
     2341 gggcccttgt acagcaacat gaacttcccc atcgtctccg tcctttcttc agcgttggct
     2401 gaacgtgtgc gacgcgccgg gacgcccgag gctacaggga gaagttgtaa gagtcgaccg
     2461 gcgcgacggg ctcgggctgc ggtggcacct cgcagcggtc cggcaagtcg gcctggaagg
     2521 cgcggaacgc ctgggactcc gaaaggacct tctgcttctc ggctgagggg aagaaggcga
     2581 tgtggacgaa ggtgcggccg tcctccttct tgaacgtcgc gtagtgcacg tcgggatcgg
     2641 cgatctcgcg caactcgtcg tagaccgcgc gcacgagcgt ctcgttctcg gcgacccggt
     2701 cggctttcac cttgtaggtc acgatgacgt tgatcaacag cgccctcctg ccttcgatca
     2761 cctgtgccag agcagcctac tacccctgtg tgtttcgacc cctacgtgca gcctgggctg
     2821 acacgaaccg gcgggcctcc gccgcccgga ggcccgcctg catatgtgac gtcccgtcgg
     2881 gcgagaagtt ccgacggatc gagaagcacg ctgcggaaac cagctctgcc gcacgagtat
     2941 caggcgtcga gcacaaccgg cccggcggag acggccgggg gtgaaccacc gagctcgggc
     3001 atggtgctac gcagccagtc ggcggctacg cgattcgact cctctgcgcc ggcctggtcg
     3061 tcacagaccg tcaccgacac ggttccgtcg cccgccgcga cgagatagta agagcgaaag
     3121 cccgggacgc cacggatcac attccggatc tcgtcggctc gggcggcgag cgcggcggcg
     3181 agggacgggt cgtcgtagct acgaacactg gcgtacatcg gctctcctcg tttggggacg
     3241 cggagatcct acgtcgcagg attcggcagc gggcgcggcg ttcgagcccg ccccccgagc
     3301 actgc
//
LOCUS       DBNGNK010000045         4276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_365317,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000045 DBNGNK010000000
VERSION     DBNGNK010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_365317"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3148)
                     /locus_tag="AC150V_01205"
     CDS             complement(<1..3148)
                     /locus_tag="AC150V_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HIR3761572.1"
                     /translation="MAFLTRPRFFGVEARSAEGWNALVPKDRDWERFYRNRWQTDRVV
                     RSTHGVNCTGSCSWKVHVKDGIVAWETQQTDYPTNGADVPEYEPRGCPRGASFSWYLY
                     SPLRVRYPYVRGVLLDLWRDALAQTGDPVEAWASIVESPEKARSYKSQRGKGGFVRSS
                     WDEVADIIAAAHVHTIRTSGPDRVAGFSPIPAMSMVSYAAGTRFLSLIGGTVLSFYDW
                     YADLPPASPQIWGDQTDVPESADWWNAGYVILWGSNIPQTRTPDAHFMTEARYRGQKV
                     VVVSPDFAGHTKFADHWLPAAAGSDGALALAMAHVIFNEFHVERRVPYFESYVKKTTD
                     MPFLVTLRARDGAYVADRFLRASDLGDTSENAEWKTVLLDDTSGEPRVPHGSIGFRWG
                     DEGMGRWNLELEDIDPALTLLGRHDELVPLDLPRFDASNGAGSVLRRGVPAKRVGGQL
                     VTTVFDLLAAQLGVARDGLPGDWPSGYDDAEPGTPAWQEPITGVDAARVIRVAREFAR
                     NAERSNGRSMIMMGAGTNHWYHSDQIYRSMLAMVLLCGCQGVNGGGWAHYVGQEKVRP
                     ISGWSTVAFAADWLRPTRQQATTPFWFLASDQWRYQRTKADHFSSPLGKGLLDDLHLA
                     DVNALAARLGWMPSYPSFDRNPLDVADEAAQAGVDPKEYVVRELQEGRLGFAADDPDA
                     PGNYPRVLTLWRANLLGSSSKGHEYFLRHVLGVEDAAVRNEEVPPELRSREVVWRDEA
                     PIGKFDLFTTIDFRMNGSAVYSDIVLPAATWYEKHDLSSTDLHPFVHSFNPAVPPPWE
                     TRTDFDVFARIAESFSRLAATHLGSRTDIVAAPLLHDTPEELAQPFGEVLDWRKGECD
                     PIPGKTMPKLITVERDYADVWNKMRKLGPLTEQLGSAVKGVPLDPAPEVEELKRSNGV
                     IDGRPSLARDVHMCEAILALSGVTNGRLAQQGFETLERRAGVPLADIAAEHADSRIVF
                     ADTQVQPRKVFASPEWSGMESRERRYSPFTINVERSLPWRTLTGRQQFYVDHAWLLEI
                     GEGLPIYRPPVDVVGTVGD"
     gene            complement(3227..3931)
                     /gene="ric"
                     /locus_tag="AC150V_01210"
     CDS             complement(3227..3931)
                     /gene="ric"
                     /locus_tag="AC150V_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HIR3761573.1"
                     /translation="MSAIPTDSLVADLVVEQPSRARVFERFGIDYCCGGKTPLEAACT
                     ERGVDLATVLEALEAQASGGPEDTDWTERSLSELCAHIVERHHGYLREELPLLRQLVD
                     KVARAHGDAHPELSDVQTTFAAVAEELEQHMAKEEQILFPACVALEEGDAGGFPFGSV
                     ENPIRLMLQEHDEVAAGLSRLRALTDTYKPPEGACTSYRSMLHRLRTLESDTHSHVHE
                     ENNILFPRAVALESAA"
     gene            complement(3951..>4276)
                     /locus_tag="AC150V_01215"
     CDS             complement(3951..>4276)
                     /locus_tag="AC150V_01215"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3761574.1"
                     /translation="ARELFANESVQVLPVLDGMRYVGAIDRASIASDASDSAAVQTLA
                     APLLPTATAATPLTRALDALDRHGASRLVVLDDDGVTYVGLVCRRSDGEQLCIDAECH
                     PHSTT"
BASE COUNT          654 a         1428 c         1495 g          699 t
ORIGIN      
        1 ggtcgccgac cgtgcctacg acgtcgaccg gagggcggta gatcggaagg ccttcgccga
       61 tctccaggag ccacgcgtgg tcgacgtaga actgctggcg gcccgtgagc gtccgccagg
      121 gcagcgatcg ctcgacgttg atggtgaagg gggagtagcg acgctcgcgc gactccattc
      181 ccgaccattc gggcgacgcg aacactttgc ggggctgcac ctgcgtgtcg gcgaagacga
      241 tccgactgtc ggcgtgctcc gcggcgatgt ccgccagcgg caccccggcg cgtcgctcga
      301 gcgtctcgaa gccctgctga gcgagacgtc cgtttgtcac gccggacagt gcgaggatcg
      361 cctcgcacat gtgtacgtcc cgtgccaggc tcggcctccc gtcgatgacg ccgttggagc
      421 gcttcagctc ctcgacctct ggggctgggt cgagcggaac gcccttcact gccgagccga
      481 gctgctccgt cagcgggccg agcttgcgca tcttgttcca gacgtccgcg tagtctcgct
      541 cgaccgtgat cagtttcggc atcgtcttgc cggggatcgg atcgcactcg cccttccgcc
      601 agtcgagaac ctcgccgaac ggctgggcca gctcttcggg ggtgtcgtgc aggagcggcg
      661 ccgccacgat gtccgtgcgc gagccgaggt gggtggcggc gagacggctg aaggactctg
      721 cgatccgggc gaagacgtcg aagtccgtcc gtgtttccca cggcggcgga acggccgggt
      781 tgaaggagtg gacgaagggg tgcaggtcgg tgctggagag gtcgtgcttc tcgtaccacg
      841 tcgccgccgg gaggacgatg tcggagtaga cggccgagcc gttcatccgg aagtcgatcg
      901 tcgtgaagag atcgaacttg ccgatcggtg cctcgtctcg ccaaacgacc tcgcgggacc
      961 ggagctccgg cggcacctcc tcgttgcgga ccgcggcgtc ctcgaccccc agcacgtggc
     1021 gaaggaagta ctcgtggccc ttgctcgacg agccgagcag gttggcgcgc cagagcgtga
     1081 gcacccgtgg atagttgccg ggagcgtccg ggtcgtccgc ggcgaagccg agccgtcctt
     1141 cctgtagctc ccggacgacg tactccttcg ggtcgacgcc ggcttgcgcc gcctcgtcgg
     1201 cgacgtcgag cgggttgcgg tcgaagctcg gatacgacgg catccagccc aggcgggcgg
     1261 caagcgcgtt cacgtcggcg agatgtaggt cgtcgagcag ccctttcccg agcggggacg
     1321 agaagtgatc ggccttcgtg cgctggtagc gccactggtc gctggcgagg aaccagaacg
     1381 gcgtcgtcgc ctgctggcgc gtcgggcgca gccagtcggc ggcgaaagcc accgtcgacc
     1441 aaccgctgat cgggcgcacc ttctcctggc cgacgtagtg cgcccagccg cctccgttca
     1501 ccccctggca gccgcacagc aacaccatcg ccagcatcga tcggtagatc tgatccgagt
     1561 ggtaccagtg gttcgtgccg gcgcccatca tgatcatcga gcgcccgttc gaccgctcgg
     1621 cgttgcgtgc gaactctcgg gcgacgcgaa tcacccgcgc ggcatcgaca cccgtgatcg
     1681 gctcctgcca ggcgggcgtt cccggctcag catcgtcgta gccggacggc cagtcgccgg
     1741 gcagtccgtc acgggcgacg ccgagctggg cggcgaggag gtcgaagacg gtggtgacga
     1801 gctgccctcc cacccgcttg gcaggaacgc cacgtcgcaa cacggagccg gcgccgttcg
     1861 aggcgtcgaa acgcggcagg tcgagcggca cgagctcgtc gtgtcgaccg agcagcgtca
     1921 gcgcggggtc gatgtcttcg agctcgagat tccacctgcc catgccctcg tcgccccagc
     1981 ggaagccgat cgagccatgc ggcaccctcg gctccccgga cgtgtcgtcg aggagaacgg
     2041 tcttccattc cgcgttttcc gacgtgtcgc cgaggtcgga ggcgcggagg aaccggtcgg
     2101 cgacataggc gccgtcgcgt gcccgtagcg tcaccaggaa cggcatgtcg gtcgtcttct
     2161 tcacgtagct ctcgaagtag ggcacgcgac gctcgacgtg gaactcgttg aagatcacgt
     2221 gtgccatggc gagcgcgagc gccccgtccg agccggccgc agcagggagc cagtggtcgg
     2281 cgaacttcgt gtgccctgcg aagtcgggtg agacgacgac gaccttctgg ccgcggtacc
     2341 gcgcctcggt catgaagtgc gcgtcgggcg tccgcgtctg cgggatgttc gagccccaca
     2401 ggatcacgta gccggcgttc caccagtctg ccgactccgg cacgtctgtc tggtcgcccc
     2461 agatctgagg tgatgcaggc ggcaggtctg cgtaccagtc gtagaagctg agcacggttc
     2521 cgccgatgag cgagaggaag cgcgtgccgg ccgcgtacga gaccatcgac atggccggaa
     2581 tcggcgagaa gccggcgact ctgtcgggtc cggaagtccg gatcgtgtgc acgtgcgcgg
     2641 cggcgatgat gtccgccacc tcgtcccagc tcgagcggac gaagccgcct ttgccgcgct
     2701 gggacttgta gctgcgcgcc ttctcggggc tctcgacgat cgacgcccac gcctcgaccg
     2761 gatcgcccgt ctgggcgagc gcatcccgcc agagatcgag caggacgcca cgcacatagg
     2821 ggtagcggac gcgcagcggc gagtacaggt accagctgaa ggaggcccca cgcggacaac
     2881 cgcgcggctc gtactcgggg acatcggcgc cgttcgtggg gtagtcggtc tgctgggtct
     2941 cccacgcgac gatcccgtcc ttcacgtgca ccttccagga gcacgagcct gtgcagttga
     3001 ccccgtgcgt agagcgcacg acgcgatcgg tctgccagcg gttccggtag aagcgctccc
     3061 agtcccgatc cttcggtacg agcgcgttcc agccctccgc cgaacgggct tccacgccga
     3121 agaagcgcgg gcgcgtcagg aaagccattc gacccatcgt gaggtcacgc ctcgaacgta
     3181 tcgcgcacgc cgcggcccgc gtgtcatcac aaacggcact ttccctctag gctgccgact
     3241 cgagggcgac ggcacgcggg aagaggatgt tgttctcctc gtggacgtga ctgtgcgtgt
     3301 cgctctcgag cgtccggagg cggtggagca tcgaccggta cgaggtgcac gcgccctcgg
     3361 gtggcttata cgtatcggtc agcgcgcgga gtctgctcag cccggcggcg acctcgtcgt
     3421 gctcttgcag catcaggcgg atcgggttct cgacagagcc gaacgggaat cctcccgcgt
     3481 cgccctcctc gagcgcgaca caggccggga agagaatctg ctcctccttg gccatgtgct
     3541 gctccagctc ctcggccacc gccgcgaacg tcgtctgcac gtcactcagc tcggggtgcg
     3601 cgtcgccgtg cgccctcgcg accttgtcga cgagctggcg caggagcggc agctcctctc
     3661 tgaggtaccc gtggtgccgc tcgacgatgt gcgcgcagag ctcgctgagg gatctctccg
     3721 tccagtccgt gtcctcgggg ccgccgctcg cctgtgcttc gagtgcctcg agcacggtcg
     3781 ccaggtcaac gccgcgctcg gtgcaggccg cttcgagcgg cgtcttgccg ccgcagcaat
     3841 agtcgatgcc gaagcgctcg aagacgcgcg cgcggctcgg ttgctcgacg acgaggtcgg
     3901 caacgaggga atcggtcggg atggcgctca tcaggggcgt ccctttctgg tcacgtggtc
     3961 gagtgcggat ggcattcggc gtcgatgcag agctgctcgc cgtcgctgcg ccgacagacg
     4021 agacccacgt atgtcacacc gtcgtcgtcg agaacgacga gccggcttgc gccatggcgg
     4081 tcgagcgcgt ccagggcccg cgtcagcggc gtcgcggcag tggcggtcgg aaggagcggc
     4141 gccgccaggg tttgcaccgc ggcggagtcg gacgcgtcgg acgcgatcga ggcgcggtcg
     4201 atcgcgccga cgtaccgcat tccgtccagg accggaagaa cctgcaccga ctcgttggcg
     4261 aagagctcgc gggcgg
//
LOCUS       DBNGNK010000046         5391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1511616,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000046 DBNGNK010000000
VERSION     DBNGNK010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1511616"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /gene="secF"
                     /locus_tag="AC150V_01220"
     CDS             <1..1117
                     /gene="secF"
                     /locus_tag="AC150V_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR3761575.1"
                     /translation="IGWRRIWLGLSTVLVVICVVAFGARGLNLGIDFEGGTQIGFKTT
                     ESVALEDVRAEAAEIGQAGAVIQGRGTPVDGKYSNFTIRTESLDRDEIATLNESLATS
                     IGATSFGVKNVSESFGRQIANSALIAIFVSLLLICAYISLRFQWKFAMPVIVAMFHDI
                     IITLGIYALVGREVTTATVAAILTVLGYSIYDTIIIFDRVRENTPLMRRASFRVIGNV
                     SLWEVIPRSLVTTFSTLLPIASLFFLGGDTLKDFAFALLVGIGVGAYSSIFVAAPLLA
                     WIKEREPEFARRKDDESGLAGVTTVGGVLTDDGKEVPEGEVALVSEAEELLAPTPAAA
                     TASESPSQPAPAGGQKPSSKRERRRQRRSTRPHGRAR"
     gene            1121..1471
                     /locus_tag="AC150V_01225"
     CDS             1121..1471
                     /locus_tag="AC150V_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761576.1"
                     /translation="MSENEATADGSERRGLEELLLAGIGWASLTADAVDELAEDLSRR
                     VGVEPEKMRAAVRDTVDSWRKEIDSGAAKPGEATDRLLGRFGLARREEVEDLALTVAQ
                     LDHRLRLLERDAKE"
     gene            1513..3162
                     /locus_tag="AC150V_01230"
     CDS             1513..3162
                     /locus_tag="AC150V_01230"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR3761577.1"
                     /translation="MATAEAPKNEQRSISRLSEISRVATKHGFGYLFVGRRREPKEEA
                     PTERGRRLREMLDELGPTFVKFGQLLSTRPDVVPPDIVAELRKLQDDVRPFAFVAVEA
                     MILAELDLTIEQAFLEFDPVPIAAASIGQVHRAVLPDGRKVVVKVQRPEAPRQIASDL
                     QLMRSAARLARDRVRAIDFVNAEELVDEFARSIRQELDYGQEARNAETFRRNFADSET
                     VVVPRVCWPYTTKRVLTLEEIRGVHVRDLDLAARSSEERRELAYRMTDAWMTMIFRHG
                     FFHGDPHPSNIIVLEDGRIGLVDFGLSGRLTDEDMARLTRLFIDAATENVDALPRRLS
                     ELGVRYPRDREDELKAEIEEIYYRYYGARLADIDPIEIIREALGLIYRMNLRLPSRFV
                     ILDKAIATLGSVGTELYPDFNVFEVARPYARRLMAERFSPRRIAVRTELELRELAGVA
                     RDIPYQVHDLLDELRDGELEIRISNPGIDDLAQHIDNATNRIAVAVVILGGLLGSAVV
                     GVLAEDGPQIMGLHLLSFVGFVISGVFGVWLLWGIFRHGRL"
     gene            complement(3221..5167)
                     /locus_tag="AC150V_01235"
     CDS             complement(3221..5167)
                     /locus_tag="AC150V_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012974718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HIR3761578.1"
                     /translation="MVVSRAGRTCVTTQRFEEIYRRSLDDPEGFWGEAAAAIDWVEAP
                     ARVLDDSSAPLYRWFSGGRLNTCFNALDRHADTDRGDQVALMYDSPVTGRQKHFTYGE
                     LRDLVARFAGTLLEQGVERGDRVVIYMPMVPEAVVAMLACARLGAVHSVVFGGFAANE
                     LAARIADATPKVVVAASCGIEPGRVVAYKPLLDEAIDLVEAKPERCILLQRPQLEAGL
                     VSGRDVDWDDALAGAQPAGCVAVEATDPLYIIYTSGTTAQPKGIVRDNGGHAVALAWS
                     MKHIYDVDPGEVYWAASDIGWTVGHSYTVYAPLLHGCTSVLYEGKPVGTPDAGAFWRV
                     ASEHDVCTLFTAPTAFRAIRQQDPEGLHLAQYDLSRLRALFLAGERCDPETLRWAEAK
                     LGIPVVDHYWQTETGWPVAANCLGIEPQPVKPGSPTLPVPGWDIRVLDADGSQLPRGE
                     SGSLVARLPLPPGCAPTLWNAEDRYHASYLARFPGFYETADAGFVDDDGYVFVMARTD
                     DIINVAGHRLSTGAMEEVLAAHPDVAECAVIGVADELKGQLPVGLMVLKAGVDRAHEA
                     IVAETVQLVRDRIGPVAAFKQAVVVGRLPKTRSGKILRGTMRRIADGETYRTPATIDD
                     PAILDEVAGALAGLGYPPAVAQEG"
BASE COUNT          822 a         1903 c         1843 g          823 t
ORIGIN      
        1 catcggctgg cgccggatct ggttgggtct ctcgacggtt ctggtcgtca tctgtgtcgt
       61 ggcgttcggc gcccgcggcc tcaacctggg gatcgacttc gagggcggca cccagatcgg
      121 cttcaagacc acggagtcgg tcgcgctcga ggacgtgcgc gctgaagcgg ccgagatcgg
      181 ccaggccggc gcggtgatcc aggggcgagg cacgcctgtg gacggcaagt actcgaactt
      241 cacgatccgt accgagtccc tcgaccgtga cgagatcgcg acgctgaacg agagtctcgc
      301 gacctcgatc ggggccacct cgttcggggt gaagaacgtc tccgagagct tcgggcggca
      361 gatcgccaac tccgccctga tcgcgatctt cgtctcgctg ctcctgatct gcgcttacat
      421 ctcgctgcgc ttccagtgga agttcgcgat gcccgtgatc gtcgccatgt tccacgacat
      481 catcatcacg ctcggcatct acgcgctcgt cggccgggag gtgacgacgg ccacggtcgc
      541 ggccatcctc acggttctgg gctactcgat ctacgacacg atcatcatct tcgaccgcgt
      601 gcgcgagaac acgcccctga tgcggcgtgc gtcgttccgc gtgatcggca acgtctccct
      661 gtgggaggtc atcccgcgct cgctcgtcac gacgttcagc acgctgctcc cgatcgcgtc
      721 gctgttcttc ctcggcggcg acacgctcaa ggacttcgcc ttcgcgctgc tcgtcgggat
      781 cggcgtgggc gcgtactcgt cgatcttcgt cgctgcgccg ctcctcgcgt ggatcaagga
      841 gcgcgagccc gagttcgcca ggcgcaagga cgacgagagc gggctcgcgg gcgtcacgac
      901 ggtcggcgga gtcctcaccg acgacggcaa ggaggtgccc gagggcgagg tggcgctcgt
      961 cagcgaagcc gaggagctgc tcgcgccgac acccgcggcg gcaaccgcgt cggaatctcc
     1021 ctcgcaaccg gccccggccg gcggccagaa gcccagctcg aaacgcgagc gtcggcggca
     1081 gcgccgctcg acgcgcccgc acggccgggc gcgctaggcg atgagcgaga acgaggcgac
     1141 ggcggacgga agcgagcgtc gcgggctcga ggagctgctg ctcgccggca tcggctgggc
     1201 gtcgctcacc gccgatgccg tcgacgagct ggccgaagat ctctcgcgcc gcgtgggcgt
     1261 cgagccggag aagatgcgcg ccgccgtccg tgacacggtc gatagctggc gcaaggagat
     1321 cgacagcggc gccgcgaagc ccggcgaggc caccgatcgg ctcctcggca ggttcgggct
     1381 cgcgcgtcgt gaggaagtcg aggacctcgc gctgaccgtg gcccagctcg accaccggct
     1441 ccggctgctc gagcgcgacg cgaaggaata gcagcggtcg ccaagcgatc ggcgtcgtgc
     1501 cagactgagt cggtggcgac ggccgaagcc cccaagaacg aacaacgcag catctcgcgg
     1561 ctctccgaga tcagccgcgt cgccacgaag cacggcttcg gctacctctt cgtcgggcgc
     1621 cggcgggagc ccaaagaaga ggcgccgacc gagcgcggcc gccgcctccg cgagatgctc
     1681 gacgagctcg ggccgacgtt cgtcaagttc gggcagctcc tgtcgacccg gcccgacgtc
     1741 gttccgcccg acatcgtcgc cgagctgcga aagctgcagg acgacgtcag gccgttcgcc
     1801 ttcgtcgcgg tcgaggccat gatcctcgcc gagctcgacc tcactatcga gcaggcgttc
     1861 ctcgagttcg accccgtgcc gatcgccgcc gcctcgatcg gccaggttca ccgcgcggtc
     1921 ctcccggacg gcaggaaggt cgtcgtgaag gtgcaacggc cggaagcgcc gcggcagatc
     1981 gcgtcggacc tgcagctgat gcgctccgct gcgcgcctgg cacgcgatcg cgtccgtgcc
     2041 atcgacttcg tcaacgccga ggagctcgtc gacgagttcg cacgctcgat ccgacaggag
     2101 ctcgactacg gccaggaggc gcggaacgca gagaccttca ggcgcaactt cgccgactcg
     2161 gagacggtcg tcgtcccgcg cgtctgctgg ccgtacacga ccaagcgcgt gctgaccctg
     2221 gaggagattc gcggcgtcca cgtgcgggat ctcgacctcg cggcgcgatc atccgaggag
     2281 cgacgcgagc tcgcgtatcg catgacggat gcctggatga ccatgatctt ccggcacggc
     2341 ttcttccacg gcgatccaca tccctcgaac atcatcgtcc tcgaggacgg tcgcatcggg
     2401 ctcgtcgact tcggcctctc cgggcgcctc accgacgagg acatggcccg cctgacgagg
     2461 ttgttcatcg acgctgcgac cgagaacgtc gacgctcttc cccgccggct gtccgagctc
     2521 ggcgttcggt acccacggga ccgcgaggac gagctgaagg cggagatcga ggagatctac
     2581 tatcgctact acggcgcgcg cctggccgac atcgacccca tcgagatcat ccgagaggcg
     2641 ctcggtctca tctaccggat gaacctccgg cttccctcgc gcttcgtcat cctcgacaag
     2701 gcgatcgcga cgctcggctc ggtcgggacg gagctctacc cggatttcaa tgtcttcgaa
     2761 gttgcgcgtc cgtacgcgcg ccgactgatg gccgagcgct tctcgcctcg gcggatcgcc
     2821 gttcgcaccg agctcgagct gcgggagctc gccggcgtgg cgcgcgacat cccgtaccag
     2881 gtgcacgacc tcctcgacga gctgcgtgac ggcgagctgg agatccgcat ctcgaatccc
     2941 ggcatcgacg atctggcgca gcacatcgac aacgccacca accggatcgc cgtcgccgtg
     3001 gtcatcctcg gcggcctgct cggctccgcc gtcgtcggtg tcctggccga ggacgggccg
     3061 cagatcatgg gcctccacct gctctcgttc gtcggcttcg tcatctccgg cgtcttcggg
     3121 gtgtggctgc tctggggcat cttccgccac gggcgcctct gaggccgctc gccggcagtc
     3181 tctcccggcc gggagccggc ctccgcgccc ggacggctcg tcagccctct tgcgccaccg
     3241 ccggtggata gccgagcccg gcgagtgcgc ccgccacctc gtcgaggatg gccgggtcgt
     3301 cgatcgtcgc gggcgtgcgg taggtctcgc cgtcggcgat ccgccgcatc gtgccgcgca
     3361 ggatcttgcc ggagcgcgtc ttgggcaggc gcccgacgac cacggcctgc ttgaaggcgg
     3421 cgaccgggcc gatccggtcg cgcaccagct gaactgtctc cgccacgatc gcctcgtggg
     3481 cacggtcgac gcccgctttc agcaccatga gcccgaccgg aagctggccc ttcagctcgt
     3541 cggcgacacc gatgacggcg cactcggcca catccgggtg cgccgccagc acctcctcca
     3601 tggcgccggt cgacagacgg tgaccggcga cattgatgat gtcgtccgtt cgagccatca
     3661 cgaagacgta cccgtcgtcg tccacgaaac cggcgtcggc cgtctcgtag aaaccgggga
     3721 agcgggcgag gtagctggcg tggtagcggt cctcggcatt ccagagggtg ggtgcgcagc
     3781 cgggcgggag cggcagccgc gccacgagcg agccgctctc gccgcgcggc agctgggacc
     3841 cgtccgcgtc gagcacgcga atatcccagc cgggcaccgg gagcgtgggc gagccgggct
     3901 tcaccggctg cggctcgatg ccgaggcagt tcgcggccac gggccagccc gtctcggtct
     3961 gccagtaatg gtcgacgacc ggaatcccga gcttggcctc cgcccagcgc agcgtctcgg
     4021 gatcacaacg ctcacctgcg aggaagagcg cacgcagccg cgacaggtcg tactgcgcga
     4081 ggtgcaggcc ctcggggtcc tgctgccgga tcgcccggaa cgccgtgggc gccgtgaaaa
     4141 gggtgcagac atcgtgctcg gaggcgacgc gccagaacgc ccccgcatcc ggcgtcccga
     4201 ctggcttgcc ctcgtagaga accgatgtgc acccgtggag cagaggtgcg tacaccgtgt
     4261 aggagtgtcc gaccgtccaa ccgatgtccg acgcagccca gtagacctcg cccgggtcga
     4321 cgtcgtagat gtgcttcatg ctccaggcca gcgcgacagc gtggccgccg ttgtcacgca
     4381 cgatgccctt gggctgagcc gtcgtaccgg acgtgtagat gatgtagagg ggatccgtcg
     4441 cttccaccgc gacgcagccg gcgggctggg cgccggcgag tgcgtcgtcc cagtcgacgt
     4501 cgcgcccgct cacgagcccg gcctcgagct gcgggcgctg caggaggatg cagcgctcgg
     4561 gcttcgcttc gacgagatcg atcgcctcgt cgagcagtgg cttgtacgcc acgacccggc
     4621 ccggttcgat cccgcatgac gccgcgacca cgaccttcgg cgtcgcatcg gctatgcggg
     4681 cggccagctc gttggccgcg aagccgccga agacgaccga gtgaaccgcg ccgaggcgcg
     4741 cgcaggcgag catcgccacc acggcctcgg gcaccatcgg catgtagatg acgacgcggt
     4801 caccgcgctc gactccctgc tcgaggagcg tcccggcgaa gcgggccacg aggtcgcgaa
     4861 gctcgccgta ggtgaagtgc ttctgccggc cggtcaccgg tgagtcgtac atgagcgcga
     4921 cctgatcgcc ccggtccgtg tcggcgtgcc ggtcgagggc gttgaaacac gtgttcaggc
     4981 ggccgccgct gaaccagcgg tagagaggag cggacgagtc gtcgagcacg cgggccggcg
     5041 cctcgaccca gtcgatcgcg gcagccgcct cgccccagaa gccctcgggg tcgtcgagcg
     5101 agcggcgata gatctcctcg aaacgctgcg tcgtcacgca cgtcctcccg gcacgcgaca
     5161 cgaccatcca cctgccgcgc cgtctgcaag gatgggctcg cgcagcgggc ttccgctggc
     5221 tcgagatgcc ggaccatttc gccggccgtc ccgacgaagg agcgcctcta atggtgtgcc
     5281 gcggcgccgg cgtgggccag cggcgcgggc cgtcagggca gcgggatcga ctccaccgcg
     5341 ccgctgacga agaggccggg ctcgggcccg ccggcgatcg tccagacacg a
//
LOCUS       DBNGNK010000047         2755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1082231,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000047 DBNGNK010000000
VERSION     DBNGNK010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1082231"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..230)
                     /locus_tag="AC150V_01240"
     tRNA            complement(157..230)
                     /locus_tag="AC150V_01240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(194..196),aa:Met,seq:cat)
     gene            complement(266..994)
                     /locus_tag="AC150V_01245"
     CDS             complement(266..994)
                     /locus_tag="AC150V_01245"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3761579.1"
                     /translation="MGRRAVGMVSDLWRFPVKSFGGERTRRAFVGPFGLLGDRRHAVI
                     DEAGQAMTARRAHALLGFTARCAEPDTGDGVQVATPSGLALPWDDLAVAEELVGALGQ
                     DAALARSPVGVHDAAPIHIVSTASLEAGARWTAGEPLDRRRFRANLVIEADDAQEFGE
                     SAWVGTMLAIGDEGPLLRVVCPTERCSVTTFDPDTLERDNRVLAGLARERENFFGVYA
                     TVARSGWVAVGDTVRIEVPTEAIA"
     gene            complement(997..1173)
                     /locus_tag="AC150V_01250"
     CDS             complement(997..1173)
                     /locus_tag="AC150V_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761580.1"
                     /translation="MAYDDEEPEPERMTDPEALERSRERIRELYEVRRAVLRWVYFGL
                     AVLAVIVIVLALTR"
     gene            complement(1295..2380)
                     /locus_tag="AC150V_01255"
     CDS             complement(1295..2380)
                     /locus_tag="AC150V_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3761581.1"
                     /translation="MTVSEMVELEEVQALLSRGQEQGFLSIDEVAKSVEGVSLDEGAA
                     EDLYRHLDEVGVELIEESEARERLAAIAEAADKPPRKLNLKADTSTDALQLFLKDVGR
                     VPLLTAKQEVELAKLIELGDLQAKKKMVEANLRLVVSIAKNYRNQGLAFLDLIQEGTL
                     GLVRAAEKFDYRKGFKFSTYATWWIRQAVARAIADKGRTIRMPVHVVEKLNKINRAER
                     KLVSELGREPSIPEIADAVKLPEAEVESIKRSAQTPVSLEKPVGDDEESEFGQFLADE
                     KAVAPEAAAETVLRNEALRDVLNSLSYRERRVLELRYGLGGEHPRTLDEVGRTFNVTR
                     ERIRQIENQSLQKLASLAEAQRLRDVA"
BASE COUNT          385 a         1001 c          942 g          427 t
ORIGIN      
        1 ggggcgccgg cggctcgagc gcttccgccc cgcaccgtct ccggcaccgt cttggtcggg
       61 cggggacgct cccgcaccgt cgtcagatgt accggagccg gcgggacatt gtgcccagga
      121 acgacgaagg cccggtggat accgggcctt cgtgagtagc gggggcagga ttcgaacctg
      181 cgaccttcgg gttatgagcc cgacgagcta ccagactgct ccaccccgcg gcgggttacg
      241 aagtgtagcc cttcggcgcg caggatcaag cgattgcctc ggtcggcacc tcgatccgaa
      301 ccgtgtcgcc gaccgcgacc catcctgagc gggcgacggt cgcgtagaca ccgaagaagt
      361 tctcccgctc gcgggcgagc ccggcgagca cgcggttgtc gcgctcgagc gtgtccgggt
      421 cgaaggtcgt caccgagcag cgctcggtgg ggcagacgac gcgcagcagc gggccctcgt
      481 cgccgatcgc gagcatcgtc ccgacccagg cggactcccc gaactcctgc gcatcgtcgg
      541 cctcgatcac gaggttggca cggaaccggc ggcggtcgag cggctcgccc gcggtccagc
      601 gcgcgccggc ctcgagcgag gcggtggaca cgatgtggat cggcgccgcg tcgtgcacgc
      661 ccaccgggct gcgcgcgagc gcggcgtcct gcccgagcgc gccgaccagc tcctcggcga
      721 cggcgaggtc gtcccagggc agggcgagcc ccgacggcgt cgccacctgc actccgtcgc
      781 ccgtgtcggg ctcggcgcag cgagcggtga atccgagcag ggcgtgcgcc cgtcgcgcgg
      841 tcatcgcctg gcccgcctcg tcgatgacgg cgtggcgccg gtcgcccagc aggccgaacg
      901 gtccgacgaa ggccctgcgg gtgcgctcgc cgccgaacga cttgacggga aaccgccaca
      961 ggtccgagac catcccgacg gcccgccgtc ccacggtcag cgggtcagcg cgagcacgat
     1021 cacgatcacg gccagcaccg ccaggccgaa gtagacccag cgcaggaccg cgcgccgcac
     1081 ctcgtagagc tcgcggatgc gctcgcgcga gcgctcgagc gcctccgggt ccgtcatgcg
     1141 ctcgggctcg ggctcctcgt cgtcgtaggc caccgaccga atgtagcgct ccgccgtccg
     1201 cggggccggt acccctctcc ggtcactcgt cgccccccgg aacgacgaag ggccccgttc
     1261 ggggcccttc gcgacgggtg agtgagcggg tggcctaggc cacgtcgcgc aggcgctgcg
     1321 cctcggcgag gctggccagc ttctggaggg actggttctc tatctgacgg atgcgctcgc
     1381 gggtgacgtt gaacgtccgc ccgacctcgt cgagcgtgcg cgggtgctcg ccgccgagtc
     1441 cgtagcggag ctcgaggacg cgccgctccc ggtagctcag ggagttgagg acgtcgcgca
     1501 gcgcctcgtt gcgcagcacc gtctcggccg cggcctcggg ggcgacggcc ttctcgtcgg
     1561 cgaggaactg gccgaactcc gactcctcgt cgtcgccgac cggcttctcg agcgacaccg
     1621 gcgtctgcgc gctgcgcttg atcgactcga cctcggcctc gggcagcttg accgcgtccg
     1681 cgatctcggg gatcgagggc tcgcggccca gctcgctgac cagcttgcgc tcggcgcggt
     1741 tgatcttgtt cagcttctcg acgacgtgca ccggcatgcg gatggtgcgc cccttgtcgg
     1801 cgatcgcgcg cgccaccgcc tggcggatcc accaggtcgc gtaggtcgag aatttgaagc
     1861 ccttgcggta gtcgaacttc tccgcggccc gcaccaggcc gagcgtgccc tcctggatca
     1921 ggtcgaggaa ggcgaggccc tggttgcggt agttcttcgc gatcgagacg acgagccgga
     1981 ggttggcctc gaccatcttc ttcttggcct ggagatcgcc cagctcgatc agcttggcga
     2041 gctcgacctc ctgcttggcc gtcagcaggg ggacccggcc cacgtccttc aggaagagct
     2101 gcagcgcgtc cgtgctggtg tccgccttga ggttgagctt gcgcgggggc ttgtcggcgg
     2161 cctcggcgat cgcggccagg cgctcgcgcg cctcggactc ctcgatcagc tccaccccga
     2221 cctcgtccag gtggcggtac aggtcctctg ccgctccctc gtcgagcgag acgccctcga
     2281 ccgacttcgc gacctcgtcg atgctcagga agccctgctc ctgaccacgg ctcaacagag
     2341 cctggacctc ctcgagctcg accatctcac tcaccgtcat gtcctcacct ccggctgaac
     2401 gccatcggag cgcgtgaacc cgttcacgca acccaccgcg gggccatccg catcgaacgc
     2461 ccgttcggta cggtaccgct tcatttcttg aagtgcaagg gacagcacgc acaacagcga
     2521 acacctatcg gcacggcggc cccgaacctt tagctcttgg tacgggggta cgggcgcgac
     2581 ggatctccct cgtgttcccg gcccccaccg ccgttcctcc ccatgctgcc ggcggatgca
     2641 accagccacg ccttaagcgc gggttaagcg ctcgtgcgga ggcggtgaag gttcccgctc
     2701 gccgtgccct agggattccg gctggagccg cccgtctcga gagaggcgag aagcg
//
LOCUS       DBNGNK010000048         4908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_867727,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000048 DBNGNK010000000
VERSION     DBNGNK010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_867727"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="AC150V_01260"
     CDS             complement(<1..731)
                     /locus_tag="AC150V_01260"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HIR3761582.1"
                     /translation="MSDIRTDPLTPEEIALYHDQGFLFPIRVLDDAQVERAQEALDDH
                     LEGRRESETYELTDPIIDDAHGEAAVGSGAASAVAETTEVERKPHSVPFLFNLWERDD
                     RFREIASNPVIAGMARQLLGSEEVVLMEDGAVVKNPVVGGKLAWHQDYAYWPLATPGA
                     VTCWIALDDVGAENGGMQVAAGSHKLGEKLPVEFGDGSSFMRAERPGIGEVRPPEEEG
                     LDVVGYELKAGECGFHHALLWHASGP"
     gene            complement(728..1960)
                     /locus_tag="AC150V_01265"
     CDS             complement(728..1960)
                     /locus_tag="AC150V_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3761583.1"
                     /translation="MATTRGETSARHVTVGSAEIHVETAGEGPPVVFLHGLGLSGALW
                     NRVRDAFEPGCHLVLADLRGAGGTRELQREELSLERWADDLSALLAQLEIRRPVIVGH
                     SLGASIALKYAIERPDDVRALVLIGTEANLSNLAPRMLASAERIESMGLEAWVEEYWS
                     QNPPFSDESLARDPGILDEYRDLLLANDPADYVRQCRAIAAAEDLSDRLESVTQPALV
                     LVGGSDDRTLPEHGRELAERLADARLVELPGVGHTLPLEAPDATAGAVNAFLREIAPR
                     NAAAGILRADTTARNPREGPFGHLDVRWVVGPDTGATLISFGQSTYPTGATHENHYHP
                     NAEEVVMVVSGRGTQIVGADALDMGAGDICFIPRDTPHRITGTSEEDLVILWAFGGAA
                     SIEQAGYVPLPDDVKELK"
     gene            complement(1992..2981)
                     /locus_tag="AC150V_01270"
     CDS             complement(1992..2981)
                     /locus_tag="AC150V_01270"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3761584.1"
                     /translation="MVDAVGEAMQQAMEADPSVIVMGEDVAGGAGRGGEKENAMGGSF
                     GATKSLYPLFGSNRVRDTPISEAGFVGAAVGAAVAGLRPVVDAMWADFTGLAFDQIYN
                     QAGKMSYMFGGQVRLPLTIRVAMGSGLSAAAQHSGTLYSIYAHLPGLKVVVPSTPHDA
                     KGLLLEAIFDDSPVLVFEHLKLYVSRGPVPAEPYRIPLGVADVRREGDAVTVVAISAM
                     VERALEAADRLATEGVAVEVIDPRTLSPLDSATIIRSVEKTGALVVVDESPPRCSVAS
                     EIAAVVSEQAFDFLNAPVRRVTAPHAPVPFTPSLEEVYAPTVERIVDAVRSVV"
     gene            complement(3008..3997)
                     /locus_tag="AC150V_01275"
     CDS             complement(3008..3997)
                     /locus_tag="AC150V_01275"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3761585.1"
                     /translation="MEVDRETLVGLYRTMLTIRLFEQRVAREFRSGEIPGFVHMYVGE
                     ESVAAGVCANLDDDDYVTSTHRGHGHCIAKGCDLDRMMAEIYGREDGLCKGRGGSMHI
                     ADFSKGMLGANAIVGGGIALATGAGLASTVRDSGQVAVAFFGDGAANQGVLHESLNLA
                     AIWKLPVIFVCENNGFAESTPAAYATSVTDVASRAAGYGIPGVVADGADVLDVYRAAR
                     TAVSRARAGQGPTLLEVKTFRFMGHFEGDPDRYRDDDERASLRDRDAIPRLRDQILAD
                     GHATAAELEEMQSEIEAAVARAVDFACASPFPDPAGLERHVYPAAAAREEAAS"
     gene            complement(4018..>4908)
                     /locus_tag="AC150V_01280"
     CDS             complement(4018..>4908)
                     /locus_tag="AC150V_01280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3761586.1"
                     /translation="EPRAGRDLPLVSPRARALLKKLGFSLEDAREIPPSGPGGRIVDR
                     DVVAWAEARSSETATAPRDAPPGPTVQSTIPLRGRRGTIARRMVDSLQTAAQLTSVLE
                     LDVSPLVGFRARLNEAGATPRIGITSIVVKLVAAALREHALLNARVTETDVELLEEIN
                     IAVAVDTSDGLVAPVVAGADELSLEEINERIVDLAARARDDTLTPADVAGGTFTVSNG
                     GTHPVDITTAILNPPQCGILWIGRIRDRPFVVDDGTIAVRPTLQACLTFDHRAIDGGP
                     AAAFLATFEGLVADLPRLPS"
BASE COUNT          723 a         1713 c         1716 g          756 t
ORIGIN      
        1 gggcccgacg cgtgccacag cagtgcgtgg tggaagccgc actcgccggc cttgagctcg
       61 tatcccacga cgtcgaggcc ctcttcctcc ggcgggcgca cctcgccaat ccccggacgc
      121 tcggcacgca tgaaggacga gccgtccccg aactcgaccg gaagcttctc tcccagcttg
      181 tgcgagcccg ccgccacctg catgccgccg ttttcggctc cgacgtcgtc gagcgcgatc
      241 cagcacgtca ccgcgcctgg tgtggcgagc ggccagtacg cgtagtcctg gtgccacgcg
      301 agcttgcctc cgacgaccgg gttcttgacg accgcgccgt cctccatcag gacgacctcc
      361 tccgatccga gcagctggcg cgccattccc gcgatgaccg gattggacgc aatctcccgg
      421 aagcgatcat cccgctccca caggttgaag agaaagggca cagagtgcgg tttcctctcg
      481 acctcggtcg tctcggcgac ggcgctcgcc gctccggatc ccaccgccgc ctcaccgtgg
      541 gcgtcgtcga tgatcgggtc ggtcagctcg tacgtctccg actcgcgcct gccctcgaga
      601 tggtcgtcga gcgcctcctg tgcccgttcc acctgcgcgt cgtcgaggac gcggatcggg
      661 aacaggaagc cttgatcgtg atagagcgcg atctcctcgg gagtcagcgg gtcggtccgg
      721 atatcgctca tttcagctcc ttcacgtcgt cgggaagcgg gacgtatccc gcctgttcga
      781 tgctcgccgc gccgccgaac gcccacagga tgacgagatc ctcctcggag gtgcccgtga
      841 tccggtgcgg cgtgtctcgc gggatgaaac agatgtcgcc tgcgcccatg tccagagcgt
      901 cagcgccgac gatctgtgtc cctcggccgc ttacgaccat caccacctcc tcggcgttcg
      961 ggtggtagtg gttctcgtgc gtcgcgccgg tcgggtaggt cgactggccg aacgagatga
     1021 gcgtcgcccc ggtgtccggc ccgaccaccc agcgcacgtc caggtgcccg aacggccctt
     1081 ccctcgggtt gcgggctgtg gtgtctgcgc ggaggatgcc cgcggccgcg ttgcggggtg
     1141 cgatctcccg gagaaaggcg ttcaccgctc cggccgtcgc gtccggggcc tcgagcggaa
     1201 gcgtgtgccc gacaccggga agctcgacga gccgggcgtc agcgaggcgc tcggcgagct
     1261 cccgtccgtg ttcgggcaac gtgcggtcgt cgctgccgcc taccaggacg agcgcgggct
     1321 gggtcacgct ctccagccga tcggacaggt cctcggccgc ggcgatcgcg cggcactggc
     1381 ggacgtagtc ggcggggtcg ttggcgagca acaggtctcg atactcgtcg aggatgccgg
     1441 gatcgcgggc gagtgactcg tcggagaacg gggggttctg cgaccagtac tcctcgaccc
     1501 aggcctccag acccatggac tcgatgcgct ccgccgaggc gagcatccgc ggggccaggt
     1561 tggagaggtt cgcctcggtg ccgatcagga cgagcgcgcg gacgtcgtcc ggccgctcga
     1621 tcgcgtactt gagagcgatg ctggcgccga gcgagtgccc gacgatcacg gggcggcgga
     1681 tctcgagctg cgcgagcagt gcgctcagat cgtcggccca acgctcgagc gacagctcct
     1741 cgcgctgcag ctcgcgcgtg cccccggcgc cgcgcaggtc ggcgagaacg aggtgacagc
     1801 cgggctcgaa ggcgtcgcgg acgcggttcc agagcgctcc cgagaggccg agtccgtgca
     1861 ggaacacgac cggcgggccc tcacccgcgg tctcgacgtg aatctccgca gagccgacgg
     1921 tgacgtgccg cgcgctcgtc tccccccgcg tcgtcgccat ctctatatcc tataactgtt
     1981 ccggcggggc gtcacacgac cgaccgcacg gcgtcgacga tccgctcgac ggtgggcgcg
     2041 tagacctcct cgagcgacgg ggtgaaggga acaggcgcat gcggcgcggt cacgcgccgg
     2101 acgggcgcgt tcaggaagtc gaaagcctgt tcggagacga cggccgcgat ttcggaggcg
     2161 acgctgcacc gaggcgggct ctcgtcgacg acgaccagcg ccccggtctt ctcgaccgag
     2221 cggatgatcg tcgcggagtc gagaggcgac agcgtccgcg ggtcgatcac ctcgaccgcg
     2281 acaccctcgg tggccagtcg gtccgctgct tcgagcgctc gctccaccat cgccgagatc
     2341 gcgacgacgg tgacggcgtc gccctcccgt cggacgtcgg cgacgcccag cgggatccgg
     2401 tagggctctg ccggcacggg gcctcgcgac acgtagagct tcaggtgctc gaacaccagc
     2461 acggggctgt cgtcgaagat cgcctcgagc aggaggccct tcgcgtcgtg gggtgtcgac
     2521 ggaacgacga ccttgagccc agggaggtgg gcgtagatcg aatagagcgt ccccgagtgc
     2581 tgtgcagccg cactcagacc gctccccatc gcgacccgaa tcgtgagtgg cagtcgaacc
     2641 tggccgccga acatgtagga catcttcccc gcctggttgt agatctgatc gaaggccagg
     2701 ccggtgaagt cggcccacat cgcgtcgacg acgggccgca ggccggcaac cgccgctccc
     2761 accgccgccc cgacgaaccc cgcctccgag atcggcgtgt cccgcacgcg attcgaaccg
     2821 aacagggggt agaggctctt cgtggcgccg aacgagccgc ccatcgcgtt ctccttctcg
     2881 ccgccgcgcc cggcgccacc ggcgacgtcc tcgcccatca cgatcaccga ggggtcggcc
     2941 tccatcgcct gctgcatcgc ctcgccgacg gcatcgacca tcgacagagt gcgctcggtc
     3001 tggacggtca cgaggcggct tcttcgcgag cggcggccgc gggatagacg tgccgctcga
     3061 gcccggccgg gtccgggaat gggctggcgc acgcgaagtc gacggcacgg gcgacagctg
     3121 cctcgatctc ggactgcatc tcttcgagct cggcagccgt cgcgtgcccg tcggcgagga
     3181 tctggtcgcg aaggcgagga atcgcatccc ggtcgcgcag actcgcgcgc tcgtcgtcgt
     3241 cgcggtagcg gtctgggtca ccttcgaagt ggcccatgaa gcgaaaggtc ttcacctcga
     3301 ggagcgtcgg cccctgaccg gcgcgtgcgc gcgagacggc agttcgtgcg gcgcggtaga
     3361 cgtcgagcac gtcggcgccg tcggcgacca ccccgggaat tccatatccg gccgcccggc
     3421 tcgcaacgtc ggtgacgctc gtcgcgtacg ccgccggtgt cgactcggcg aagccgttgt
     3481 tctcacagac gaagatcacg ggcagcttcc agatcgccgc gaggttgagg ctctcgtgga
     3541 ggacaccctg gttcgcggcg ccgtcgccga agaacgccac cgcgacctgg ccgctgtcac
     3601 gaacggtcga cgccagtccc gccccggtcg cgagtgcgat gcccccgccg acgatcgcgt
     3661 tggcgccgag cattcccttc gagaagtccg cgatgtgcat cgagccgccg cgccccttgc
     3721 agaggccgtc ttcgcgcccg tagatctcgg ccatcatccg gtcgaggtcg cagcccttgg
     3781 cgatgcagtg cccgtggccg cgatgcgtgc tggtcacgta gtcgtcgtcg tcgaggttgg
     3841 cgcagacacc cgctgccacg ctctcctcgc cgacgtacat gtgcacgaag ccgggaatct
     3901 cgccgctccg gaactcgcgc gccacgcgct gctcgaagag ccggatcgtg agcatcgtgc
     3961 ggtagaggcc gaccagcgtc tcacggtcga cctccatcgc attctcgggt gcggcgctca
     4021 agacgggagc cgaggcaggt cggcgacgag cccctcgaag gtggccagga aagcggccgc
     4081 ggggccgccg tcgatcgcgc ggtggtcgaa tgtgaggcag gcctgcaggg tcgggcgcac
     4141 ggcgatcgtg ccgtcgtcga ccacgaaggg gcggtcgcgg atgcggccga tccagaggat
     4201 cccgcactgg ggtggattga ggattgcggt ggtgatgtcg acggggtggg tgccgccgtt
     4261 cgagaccgtg aacgtgccgc cggcgacgtc cgcgggcgtg agcgtgtcgt cgcgggcacg
     4321 cgccgcgaga tccacgatgc gctcgttgat ctcctccagc gagagctcgt cggcgccagc
     4381 gacgacgggg gcgacgagtc cgtcggacgt gtcgacggcg accgcgatgt tgatttcctc
     4441 gagcagctcc acgtcggtct cggtgacccg cgcgttcagg agcgcgtgct cgcgcagcgc
     4501 cgccgcgacg agtttcacga caatcgaggt gatcccgata cggggcgtcg caccggcctc
     4561 gttcaggcgg gcccgaaagc cgacgagcgg tgacacgtcg agctcgagca ccgacgtgag
     4621 ctgagcggcg gtctggaggc tgtcgaccat gcgccttgcg atcgtgccgc gacggccgcg
     4681 aagagggatc gtgctctgca cggtcggccc cggcggtgcg tcgcggggcg cggtggccgt
     4741 ctcactcgac cgcgcctcgg cccacgcgac gacatcgcgg tcgacgatcc gcccgcccgg
     4801 gcccgacggc ggtatctcgc gcgcgtcctc gagcgagaac ccgagcttct tgagcagcgc
     4861 acgcgcgcgc ggcgacacga gcggaaggtc gcggccggcc ctcggttc
//
LOCUS       DBNGNK010000049         5524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1154199,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000049 DBNGNK010000000
VERSION     DBNGNK010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1154199"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="AC150V_01285"
     CDS             <1..436
                     /locus_tag="AC150V_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR3761587.1"
                     /translation="DLQEIKGSRLESIPADQLEDAFVHAFRGVVPNDKRFLGGETIGS
                     LFDRVLPALDRLLADDGWDTVLAVLHGAVNRGILSYALTGERMFLGHFEQAPGCVNVL
                     DVGEEWIVRAVNIAPSDLAHSSTRMTTMEHYWEQYRPNASHG"
     gene            complement(502..1767)
                     /locus_tag="AC150V_01290"
     CDS             complement(502..1767)
                     /locus_tag="AC150V_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3761588.1"
                     /translation="MKGIAMRKGLLLGLVLALVGVFATSAATAPSQTPGVTSKTITIG
                     GTFPLTGPASLYSPIPSAMKAYFSYVNARKGSDGRRGVNGRQIVFKFYDDGYNPANSV
                     QLTRRLVEQDKVFAVVGSLGTEVNLAIRPYLNSKKVPQLLVASGASTWGRDWKKFPFT
                     TGWQPDYELEGRIYGQAIARNSPNSKIAVLYQNDDYGRDYLQGLEAGLGAKKSNIVAE
                     SYEVTAADVRSQMAKLRASGAKVFAIFATPKFTIQAYVFARALGWNPPVIYTNSVSGA
                     DVFLTTAQKSGAGDLVNNTFSVQYAKDPNSPVWANDSAMKLYRNIMSKYFPNGSSAAA
                     QANQLNYYGVAVAHAFVQLLEAAGKNPTRDSLMKAARNWKQVNPFLLPGNVQLTNSKS
                     QYPLRCEQIVKYTDGTFVRVSKLKCPASS"
     gene            complement(1829..3622)
                     /locus_tag="AC150V_01295"
     CDS             complement(1829..3622)
                     /locus_tag="AC150V_01295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR3761589.1"
                     /translation="MATTELTGAEDVSWDLSDLYAGPEDPDLQGEVDDAKSSGEAFRA
                     RYHGKVGGLDAAGLAAAVEERERIDSAVARALTFAHLRFATNMADPERGALVAKLQEQ
                     AASLEAELLFFALEWAAVEEAAATELLQSPELDHWRHFLTSLRKYRPFLLSEPEERIV
                     TEKAVSGSAAWSRLYDELIGALRIELDGEEVALETAMARLYASDREVRRETAQAVTTA
                     LEHGLRTRVFVLNTLLLDKSIDDRLREYPSWISSRNLANETTDEAVEALIEATVSRYD
                     VPQRYYRLKAKLLGVERIAHFDRFAPVAETGANTSWDEARRTVVDAYSSFSDEAGAIV
                     ERFFVDDWIDAPVRPDKRTGAFCATTVPGAHPYVFMNFTGDRRSVLTLAHELGHGLHG
                     ALAQPLGLYNASTPLTTAETASVFGEALTFRNLLESETDPRRRLDLLTGRIEDAIATV
                     FRQVSMNRFEDAIHNERRTAGELSPDRLNELWLEEQDALFADSVDTDGYAPWWSYVTH
                     FAAVPGYVYAYAYGYLFSLAIFRRYEQEGDVLVEPYFDLLRAGGSQPPEDLARLVGLD
                     LTDPSIWASGIDALSEQLDEAERLAAEIGLG"
     gene            complement(3671..>5524)
                     /locus_tag="AC150V_01300"
     CDS             complement(3671..>5524)
                     /locus_tag="AC150V_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR3761590.1"
                     /translation="NPVDWYPWGDEALERARGENRPILLSVGYSACHWCHVMEHESFE
                     DTATAALMNDLFVNVKVDREERPDIDAVYMDATVAMTGQGGWPMTVFLTPAGEPFYAG
                     TYFPPEPRHGMPSFRQVLQAVGRAWSEQHEDVVKQAARLVGALGGSVDARPSAEPLGD
                     DLLATAERGIAQTFEPAYGGFGRAPKFPPASTLEFLLRRRSEDALQMVTQTLDGMAAG
                     GMYDVVGGGFHRYSVDERWLVPHFEKMLYDNALLASAYLHAWMVTGVERYRDVAAETV
                     DYMLRDLLLPGGGLASAEDADTEGVEGLTYTWTEEEAAQAGLDVALLQPFEHGRSIIR
                     GDLDAETRSRLLAIRRRRPQPLRDDKAIASWNGLALAALAEAGRRLDRRDWVEAAAGV
                     AEFLLGPLSGEDGRLLRSFREGRTSGDGYLDDYANAAHGLLELHVATGERRWLVEARR
                     LVLLAVELFADEVNGGFFLAERERNELVAASKPLDDNPLPSGNSMLAHVILRIARMWG
                     DDALESRAVGVFRLIEPATRRVPGAFGWALCALHLWLSTPRELAIAGPVSSDVARAAL
                     APFDPTAVVAVGPADEVPLLAGKGLVDGRPAVYVCERFACKAPVTDPAELQ"
BASE COUNT          899 a         1856 c         1871 g          898 t
ORIGIN      
        1 cgacctgcag gagatcaaag gaagccggct cgagtcgata ccggccgacc agctcgaaga
       61 cgcgttcgtg catgcgttca gaggggtcgt cccgaacgac aagcgcttcc tcggcggaga
      121 gacgatcggc tcgctcttcg atcgcgttct cccggcgctc gatcggcttc tcgcggacga
      181 cggctgggac acggtgctcg ccgtgctgca cggggcggtc aaccgcggga tcctctcgta
      241 tgcgttgacc ggggagcgga tgtttctcgg ccacttcgag caagcgcccg gatgcgtcaa
      301 cgtgctcgac gtgggcgaag agtggatcgt gcgggcggtg aacatcgcgc cgtccgacct
      361 ggcgcactcg tcgacgcgaa tgacgacgat ggagcactac tgggagcagt accggccgaa
      421 cgcgtcacac ggctagccct gcaaggaaga gagggccggc attgccggcc ctctcagcga
      481 ttgcgcgtct gcgcgcttgg ctcagctcga agccgggcac ttcagcttcg acaccctgac
      541 gaacgtcccg tccgtgtact tgacgatctg ctcgcagcga agcggatact ggctcttcga
      601 gttcgtcagc tgcacgttgc cggggagcag gaacgggttg acctgcttcc agttgcgcgc
      661 ggctttcatg agcgagtccc gcgtcgggtt cttccccgcc gcttcgagca gctggacgaa
      721 ggcatgcgcg accgcgacgc cgtagtagtt cagctggttc gcctgggcgg cggcgctcga
      781 gccgttcggg aagtacttcg acatgatgtt gcgatacagc ttcatcgcgc tgtcgtttgc
      841 ccagaccggg ctgttggggt ccttcgcgta ctgaacgctg aaggtgttgt tgaccaggtc
      901 tccggcgccg gacttctgcg cggttgtcag aaacacgtcg gctccggaca cggagttcgt
      961 gtagatgacc ggtggattcc agccgagcgc ccgggcgaag acgtacgcct ggatcgtgaa
     1021 cttcggtgtc gcgaagatcg cgaacacctt ggcaccgctc gcacgcagct tggccatctg
     1081 cgagcgaacg tccgcagccg tgacctcgta cgactcggcc acgatgttcg acttcttggc
     1141 gccgagacca gcctccaggc cttgcaggta gtccctgccg tagtcgtcgt tctggtacag
     1201 gacggcgatc ttcgagttcg gactgttgcg ggcgatcgcc tgaccgtaga tacgcccctc
     1261 cagctcgtag tccggctgcc agcccgtggt gaacgggaac ttcttccagt cccggcccca
     1321 cgtcgatgcg cccgacgcga cgagcaactg cggaaccttc ttcgagttga ggtacgggcg
     1381 aatcgccagg ttcacctcgg tccccagcga gccgacgacc gcgaacacct tgtcctgctc
     1441 gacgaggcgc cgggtcagct gaacgctgtt agccgggttg tagccgtcgt cgtagaactt
     1501 gaagacgatc tgtcggccgt tgacgccgcg cctgccgtcg gaccccttcc gcgcattgac
     1561 gtagctgaag tacgccttca tcgcgctggg aatgggtgag tacagcgacg cgggccctgt
     1621 gagcgggaac gtaccgccga tcgttatcgt cttcgacgtg acgccgggcg tctgactcgg
     1681 tgccgtcgcc gccgacgttg cgaagacgcc cacgagcgcc agaacgagcc cgagaagcag
     1741 cccctttcgc atagctatcc ctttcatcga tgtgtcgacc cgggcgacca tatcgcccgg
     1801 taacaaagac gacaaatagc gcggctcgtt agccgaggcc gatctcggcg gcgaggcgct
     1861 cggcctcgtc gagctgctcc gacagcgcgt cgatcccgga cgcccagatc gaaggatcgg
     1921 tgagatcgag gccgacgagg cgggcgaggt cctcgggcgg ctgcgagccg ccggcgcgga
     1981 gcagatcgaa atacggctcg acgagcacgt cgccctcctg ctcgtaacgg cggaagatcg
     2041 cgagggagaa caggtacccg taggcgtagg cgtagacgta gccgggcacg gcggcgaagt
     2101 gcgtcacgta gctccaccag ggcgcatatc cgtcggtgtc caccgagtcc gcgaagagcg
     2161 cgtcctgctc ctccagccag agctcgttga gcctgtccgg cgagagctca cccgccgtgc
     2221 gccgctcgtt gtggatcgcg tcctcgaacc gattcatcga gacctgcctg aatacggtgg
     2281 cgatcgcgtc ttcgatcctg ccggtgagca gatccagcct gcgacgcggg tcggtctcgg
     2341 actcgagcag gttgcggaac gtcagcgcct ctccgaacac cgatgcggtc tccgcggtcg
     2401 tcagcggcgt cgacgcgttg tagaggccga ggggttgcgc gagcgcgccg tggaggccgt
     2461 gtccgagctc gtgcgccagc gtcaggaccg accggcgatc gcccgtgaag ttcatgaaga
     2521 cgtacggatg cgcgcccgga accgtcgtcg cgcagaaggc gccggtgcgc ttgtcggggc
     2581 ggacgggcgc atcgatccag tcgtccacga agaaccgctc cacgatcgcg ccggcctcgt
     2641 cggagaagga ggagtacgcg tcgacgaccg ttcgccgtgc ttcgtcccag ctcgtgttgg
     2701 cgccggtctc ggcgacgggg gcgaagcggt cgaagtgggc gatgcgctcg acgccgagga
     2761 gcttcgcctt cagccggtag tagcgctgcg gcacgtcgta ccgcgacacg gtcgcctcga
     2821 tcagcgcctc gacggcctcg tccgtcgtct cgttggcgag gttgcgcgaa gagatccagc
     2881 tcggatactc ccgcagccgg tcgtcgatcg atttgtccag cagcaaggtg ttgagcacga
     2941 agacccgcgt ccgcagcccg tgctcgagag cggtcgtgac cgcctgtgcg gtctcgcggc
     3001 ggacttctcg gtccgacgca tacagacgag ccatggccgt ctcgagggcc acctcctcgc
     3061 cgtcgagctc gattctgaga gcgccgatca gctcgtcgta cagccgcgac cacgccgccg
     3121 accccgacac ggccttctcg gtgacgatcc gctcctcggg ctcggaaagg agaaacggcc
     3181 ggtacttgcg cagcgaggtg aggaaatggc gccagtgatc gagctccggg ctctgcagca
     3241 gctcggttgc ggccgcctcc tcgacagcgg cccattcgag cgcgaagaag aggagctcgg
     3301 cctcgagtga agcggcctgt tcctggagct tggcgacgag cgcgccccgc tcgggatcgg
     3361 ccatgttcgt cgcaaagcgg aggtgggcga acgtcagagc ccgggcgacg gcggagtcga
     3421 tccgctcgcg ctcctcgacg gcagccgcaa gcccggccgc gtccagcccg ccgaccttgc
     3481 cgtgataccg ggcccggaag gcctctccgg acgacttcgc gtcgtcgact tccccctgaa
     3541 gatccggatc ctcgggaccc gcatagaggt cggagagatc ccaggaaacg tcttcggcgc
     3601 ccgtcagctc ggtcgtcgcc acggacgaga ccctagcgac tggtgtcggc gcggcgcagg
     3661 cgctcccgac tcactgcaac tccgccggat cggtcaccgg cgccttgcag gcgaatcgct
     3721 cacagacgta cacggcggga cgaccgtcga cgagtccctt gcccgccagg agcggcacct
     3781 cgtccgccgg accgacggcg acgacggcgg tcgggtcgaa gggcgcgaga gcggcgcgcg
     3841 cgacgtcgga cgagaccggc ccggcgatcg cgagctctcg aggcgtcgag agccagaggt
     3901 ggagcgcgca gagcgcccag ccgaacgctc ccgggacgcg ccgggtcgcg ggctcgatga
     3961 gccggaacac gcccacggct cgcgactcga gggcgtcgtc cccccacatc cgggcaatcc
     4021 ggagaatgac gtgcgcgagc atcgagttcc ccgacggcaa cgggttgtcg tcgagtggct
     4081 tcgaggcggc gaccagctcg ttccgctccc gctcggccag gaagaagccg ccgttcacct
     4141 cgtccgcgaa aagctcgacg gcgagcagga cgaggcgccg ggcctcgacc agccaacggc
     4201 gctcgcctgt cgccacgtgc agctcgagca ggccgtgcgc ggcatttgcg tagtcgtcga
     4261 ggtagccgtc gccgctcgtt ctgccctcac ggaagctccg tagcagccgc ccgtcttccc
     4321 cagagagggg cccaagcaga aactccgcga cgccagctgc cgcctcgacc cagtctcgtc
     4381 tgtcgagccg gcgcccggcc tcggcgagcg ccgcgagggc gagcccattc cacgaagcga
     4441 ttgccttgtc gtcgcgtagc ggttgcgggc gtctcctcct gatcgccagc agccgggagc
     4501 gagtctcagc gtcgagatcg ccgcggatga tcgagcggcc gtgctcgaag ggctggagca
     4561 gtgccacgtc gagacctgct tgggcggcct cttcttccgt ccaggtgtag gtcagtccct
     4621 ccactccttc ggtgtcggcg tcctcggccg acgcgagccc gcctccgggc aagaggaggt
     4681 cgcgcagcat gtagtcgacg gtctcggcgg ccacgtcccg gtagcgctcc acgccggtga
     4741 ccatccaggc atggagatag gcggaggcca gtagcgcgtt gtcgtagagc atcttctcga
     4801 aatgcggcac gagccagcgt tcgtcgaccg aatagcggtg gaaaccacct cccacgacgt
     4861 cgtacatgcc gccggccgcc atcccgtcga gcgtctgcgt gaccatctgc agggcgtcct
     4921 cgctccggcg tcgaaggaga aactcgagcg tcgaagcggg cgggaacttc ggtgcgcgcc
     4981 cgaagccgcc gtaggccggc tcgaacgtct gagcgatgcc acgctcggcc gtcgcgagca
     5041 agtcgtcgcc gaggggctcg gcggaggggc gcgcgtccac tgagccgccc agcgcgccga
     5101 cgagccgcgc agcctgcttg acgacgtcct cgtgctgttc gctccaggca cgcccgaccg
     5161 cctgcagaac ctgacggaag ctgggcatcc cgtgccgggg ctccggtggg aagtacgtcc
     5221 ccgcatagaa cggctcgccg gccggagtca ggaagacggt catcggccac ccgccctgcc
     5281 ccgtcatcgc cacggtggcg tccatgtaga ccgcatcgat gtcgggacgc tcctcgcggt
     5341 cgaccttcac gttgacaaag agatcgttca tgagggcggc cgtagcggtg tcctcgaacg
     5401 actcgtgctc catcacgtgg caccagtggc aggcgctgta cccgaccgag agcagaatcg
     5461 gacgattctc gcctcgggcg cgttcgagcg cctcgtcccc ccacgggtac cagtcgaccg
     5521 ggtt
//
LOCUS       DBNGNK010000050         9265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1154433,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000050 DBNGNK010000000
VERSION     DBNGNK010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1154433"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="AC150V_01305"
     CDS             <1..801
                     /locus_tag="AC150V_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR3761591.1"
                     /translation="TEQMLRHFIQKVHQHRFAHPRVVVCVPSGVTGVEKRAVEEATLS
                     AGARQAYLIEEPMAAAIGAGLPVAEPTGNMIVDVGGGTTEVAVISLGGIVVSQSLRVG
                     GDEMDDAIVNHIKKEYKLLVGQQTAEEIKLEIGSAFRMKEELQAEVRGRDMLTGLPKT
                     VIVSSDEIRRALDEPVSQIIDAIKSTLDKTPPELAADIMDRGIVLAGGGALLHGLDER
                     LRHETQMPVHLAESPLTCVAVGSGRSLEEFEAIHRANRSRARRNGSRR"
     gene            940..1794
                     /gene="mreC"
                     /locus_tag="AC150V_01310"
     CDS             940..1794
                     /gene="mreC"
                     /locus_tag="AC150V_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR3761592.1"
                     /translation="MRRRLVVGVLVVLALVLITLSYSQGGSGPVGDAQDVAASALRPF
                     QVATERVAQPFRDAYSWAESLVDARNEARELAKENERLRQQLLQNQAAARENDRLRSL
                     LTFRDGPAFPRDYDGLAAAVVARPSQAFAQAIVVAVGSKDGVEPNAPVVTADGLVGLV
                     TRVGTRTARVRLLVDEQSAVSALDANTDASGIVRHGKGARSTLILDRVPKEADVRVGD
                     SIVTAGWRSGPFSSLYPRGIPIGTVTSVGQTDTDLYKQVQVQPFADFGAVDSVIVLVR
                     KPEEGALP"
     gene            1791..2312
                     /gene="mreD"
                     /locus_tag="AC150V_01315"
     CDS             1791..2312
                     /gene="mreD"
                     /locus_tag="AC150V_01315"
                     /inference="COORDINATES: protein motif:HMM:NF016019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR3761593.1"
                     /translation="MRAALWIAALVFVAAILQAVIFSSVVILGAAPDVLLVTLISIAL
                     LRGSIAGAAAGFVGGLLVDVATLGTMGVTALLLTLAGYWAGRYGETTGRDRAHAPLLS
                     ILVITLLVGVGSYFVYFLLGEDVSAQVALVTALLPSLLLNLIAAVPIYALSRRLLKVS
                     ELRPSSDEVELVV"
     gene            2305..4344
                     /gene="mrdA"
                     /locus_tag="AC150V_01320"
     CDS             2305..4344
                     /gene="mrdA"
                     /locus_tag="AC150V_01320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR3761594.1"
                     /translation="MSSPTSPYGEPRSGSSRFLPPDPKVQEPYRLTPRLALRVGILGA
                     IALVLFAVLFFRLWSLQVLAGDSYLDAAQNNQLRQVRLEAPRGTIVDRRGRVIVGNVP
                     GTAVKLWVGDMPKQGRYRMIQRLATILRLSPIEIARKVDERASDPLTPITLRTAAGNE
                     QVMYLYEHQSEFPGVSIEQTYLRDYRYKALAAQLLGSVGEISADELRRKRPEGYLLGD
                     TIGKSGVEAGFDTYLRGQAGLAQIRVDSLGQPQSGLEVRRDARPGYAVRLTLDARLQR
                     AAERALVYGIETAKENESYFANGGAIVALDPRDGAVLAMASYPTFKPSVWVGTIDEKK
                     IAPLIKEKAAREANYPGVNRATSGLYPPGSTWKPVVALAAMQEHMLSPFDSIQCTPTA
                     TYGLDEQEFRNWNPYVNKPMTLPVALAESCDTYFYEIGNRFYERGLSERAFWTRLQDW
                     AGRFGFGASSGLDIGGEAVGLLPTPLWRKQTFKSDWDRAWNPGDLIQFAIGQKDLTVT
                     PLQMARFYAMIANGGKLVTPYIVSSVEQPETEGQGPVVLRSFAAEAPRSAGVDPGALT
                     VVREGLYAATHSRDGTSSGVFANYPVPIAGKTGTAEKVVPLPGYPADHLEDQSWWCGW
                     GPYNLESYNDKSPLVVCALIENGGHGSTAAAPAALKLFETWFGVEAGEQILVATD"
     gene            4354..5547
                     /gene="rodA"
                     /locus_tag="AC150V_01325"
     CDS             4354..5547
                     /gene="rodA"
                     /locus_tag="AC150V_01325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR3761595.1"
                     /translation="MTDYAGHTSSRPRIRRRPEAAGVGAFVRRLDWVLVLAVGALVAY
                     GLWAVDGITRFDVAGDPDYYVVRQAVAAGIGLAALLVALLTPPALLRRHWRALYGLTV
                     VLMVLVFVLAETVRGAKRWIDVGPFQFQPSEFGKVLLVLALAGFLVERASRITDVRTV
                     ATAIALAALPIALVFLQPDLGTAMVYTAALASVLFIAGVRWPHLAILLALAALVAVSV
                     VWLLPSAGVEVLKPYQTARLTGFANPDTDPGGLTYNVTQSMTAIGAGGLDGRGVTGAS
                     QTRLAYLPEHATDFVFASLAEQRGFFGAAILLLLYLLVVWRGLRIVTLAGDLFSAIVA
                     GGIVFAFLFQIFVNVGMTMGIAPVTGIPLPFVTVGGSALVANLIVMGLLLSIHARGRM
                     PNGRR"
     gene            5574..6314
                     /locus_tag="AC150V_01330"
     CDS             5574..6314
                     /locus_tag="AC150V_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761596.1"
                     /translation="MRALRESRENAEIERTLLVVGVMADVLARELTSGGDESLARTGG
                     ALDDAAGVVVVLSGAPSDGEVDLLRQAQRAGAATVVVQLQPGPMDDIPYVLAVDVVDV
                     PPGAGFPVDEVVRALTRRMGEHVVPIAKALPRFRAPAARELTRSIATRNATLAFEPWG
                     SRIHLPILLANQVRLAFDIAAAHGREIGATRLPDAATTLGLAVGLRAASRRARQHVPG
                     RRLVNTAVAYGGTRAVGELATLRARFGP"
     gene            6705..>9265
                     /locus_tag="AC150V_01335"
     CDS             6705..>9265
                     /locus_tag="AC150V_01335"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR3761597.1"
                     /translation="MPWFRWKKNKQDKTVAAVPPAVTEEVPARAEPETARAAVTEAAP
                     SEAVAPKRKRRRGSRGGRGRKKSTAESGAKSASTEASTQDRKTSAAKSKSREPAKRAT
                     SRRRQAPRRAPLPAAKRELVISVDAGEQRVAVLEDERVAEVYLERPERRSIAGNIYLG
                     VVDNVLPGMEAAFVEIGLEKNGFLYVDEIVVPELEGKRHGKRIQDLISRGEEILVQAV
                     KDPMKTKGARLTTEISLPGRFLVFVPNGEGLGVSRRLEDAERQRLKEIIKEIVPKDGG
                     VIVRTAADGASAEDIERDLVFLERLWKHIQARMKESKAPALVYREAELPLRIVRDLFA
                     GDFVAATIDHERTFKRIVGYLKKTSPHMLERVHRYKEKTPLFEAHGIDAEIESTLDRR
                     VNLPSGGYLIFDYAEAFTVIDVNTGRFVGSRSKSSTARLEDTITKNNLEAVKEVVRQL
                     RLRDIGGIIVIDFIDMANPKNRASVEEALRSELERDRTKTYVVEISPLGLVEMTRQKV
                     TEGPREILTRKCEVCAGDGVVVSDQTVAIEVERKLRALAAGGARVQAYRVAVHPRVRA
                     LVAGPGGRRLAEIEEVARRRFYLEEPDNGHVHIDHFDVLAQGKRDVLAPDGDVQEDVE
                     MSVKLTEVDRFDSSSAVGRVGDVEIVVADAASLVGKSAKVVPGRVLAGAAYAVLADAQ
                     DGDGSITFESEAEKPTRAPAKTKRSSAVKKETDAGGDEKSTPRPKPAKTAKAKEKPAR
                     QKKVAEPATGDEAASAGEEPEATDADRADGKASQATTPRKRTRRGSRGGRRRRKPATS
                     GAAAADGAAGDGKAEAEGAAEAAKGKTPKRAPKIHVPDAAPAENGGTPAETASAES"
BASE COUNT         1469 a         3071 c         3274 g         1451 t
ORIGIN      
        1 accgagcaga tgctccgcca cttcatccag aaggttcacc aacaccggtt cgcgcacccg
       61 cgcgtcgtgg tgtgcgtgcc gtcgggcgtc acgggagtcg agaagcgcgc ggtcgaggag
      121 gcgacactgt cggcaggagc gcgtcaggcc tatttgatcg aggagccgat ggccgccgcg
      181 atcggcgcgg gcctacccgt ggccgagccg accgggaaca tgatcgtcga cgtcggcggc
      241 ggtacgaccg aggtcgccgt catctcgctc ggcggcatcg tcgtctcgca gtcgttgcgg
      301 gtcggcggcg acgagatgga cgacgcgatc gtcaatcaca tcaagaagga gtacaagctg
      361 ctcgtcgggc agcagacggc cgaggagatc aagctcgaga tcggctccgc cttccggatg
      421 aaggaggagc tccaggccga ggtccgaggc cgcgacatgc tcaccggcct cccgaagacc
      481 gtgatcgtct cctcggacga gatccgcaga gcgctcgacg agccggtttc gcagatcatc
      541 gatgcgatca agtcgacgct ggacaagacg ccgccggagc ttgcggccga catcatggat
      601 cgcggcatcg tcctcgcagg cggcggcgcg ctgttgcacg gtctcgacga gcgccttcgg
      661 cacgagaccc agatgccggt gcatctcgcc gagagcccgc tcacgtgtgt cgcggtcggg
      721 tccggccgta gcctcgagga gttcgaggcg atccaccggg cgaaccgctc gcgcgcgcgc
      781 cggaacggca gccgcagata gcccgcggag aggggggagc ggccatgcgc gccgctacac
      841 tggcccactg tgccccgtga ccgcaccgaa cgagcggtgc tgggcgtgag tgtccgacga
      901 cccgccgctg gccagttttc ctcacgaagc tcgactgctt tgcggcgtcg ccttgtcgtg
      961 ggggtgctcg tcgtgcttgc gctcgtgctg atcacgctct cctacagtca gggcggctcg
     1021 gggccggtgg gagacgcaca ggacgttgcg gcgagtgccc tgaggccgtt ccaggtcgcc
     1081 accgagcgcg tcgcccagcc gttccgcgat gcgtactcgt gggcggagag tctcgtcgac
     1141 gcgcggaacg aggcgcgcga gctggcgaag gagaacgagc ggctccgcca gcagctgctc
     1201 cagaaccagg ctgcggcgcg agagaacgat cggctccgtt ccctgctcac gttccgggac
     1261 gggcccgctt tcccccgcga ctacgacggg ctcgcagccg ccgtcgtggc acgcccctcg
     1321 caggccttcg cgcaggcgat agtggtcgcc gtcggctcca aggacggggt cgagccgaac
     1381 gcgcccgtcg tcaccgccga tgggcttgtc gggctcgtca cccgtgtcgg cactcggacg
     1441 gctcgcgtcc ggctgctcgt cgacgagcag agcgccgtct cggctctcga tgcgaacacc
     1501 gacgcgtcgg ggatcgtccg acacgggaag ggcgcccgct cgacgctcat cctcgaccgc
     1561 gtcccgaagg aggcagacgt tcgggtcggt gactcgatcg tgacggccgg ttggcgatcc
     1621 gggcccttct cctcgctcta tccgaggggc attccgatcg ggacggtcac gagcgtgggg
     1681 cagacggaca cggatctcta caagcaggtg caggttcagc ccttcgccga cttcggtgcg
     1741 gtcgactcgg tgatcgtgct cgtccggaag cccgaggaag gtgcgctgcc gtgagagccg
     1801 cgctctggat cgcagctctc gtcttcgtcg ccgcaatctt gcaggcggtc atcttctcgt
     1861 ccgtcgtcat cctcggcgcc gccccagacg tgctcttggt gacgctgatc tcgatcgcgc
     1921 tcctgcgggg atcgatcgcc ggtgcggcgg cgggcttcgt cggcggtctc ctcgtggacg
     1981 tcgcgaccct cggcacgatg ggcgtgacgg cgctgctgct gacgctcgcc ggctactggg
     2041 cggggcggta cggcgaaacg acgggtcgcg accgcgccca cgcaccgctc ttgtccatcc
     2101 tggtgatcac cctgctcgtc ggcgtcggct cgtacttcgt gtacttcttg ctcggtgagg
     2161 acgtctcggc ccaggtggca ctcgtgaccg cgctcctgcc gagcctgctg ctcaacctca
     2221 tcgccgccgt tccgatctac gcgctcagcc ggcgcctgct caaggtgtcg gagctcaggc
     2281 cctcgagcga cgaggtcgag ctcgttgtct agccccacct cgccgtacgg agagccgcgc
     2341 tcgggctcgt cgcgcttcct gccccccgac cctaaggtgc aagagcctta ccgcctgacc
     2401 ccgcggctcg ccctgcgcgt gggcattctg ggcgcgatcg cgctcgttct cttcgccgtg
     2461 ctcttcttcc ggctctggtc gctccaggtg ctggcgggcg attcctacct tgacgccgcc
     2521 cagaacaatc agctgcgaca ggtccggctc gaagctccgc gaggaacgat cgtcgaccgg
     2581 cgggggcgcg tgatcgtcgg caacgttccg ggtacggccg tgaagctctg ggtcggcgac
     2641 atgccgaagc agggtcgcta ccgcatgatc cagcgcctgg caaccatcct ccggctctcc
     2701 ccgatcgaga tcgcccgcaa ggtcgacgag cgcgccagcg atccgctcac cccgatcacg
     2761 cttcgaacgg ccgccggcaa cgagcaggtc atgtacctgt acgagcacca gagcgagttt
     2821 ccgggcgtct cgatcgagca gacctatctg cgcgactacc ggtacaaggc gctcgcggcg
     2881 cagttgctcg gctcggtcgg ggagatctcg gccgatgagc tgaggcgcaa gcgacccgag
     2941 ggatacctcc tcggggacac gatcgggaag tcgggtgtag aggccggctt cgacacgtac
     3001 cttcgcggtc aggcgggtct cgcgcagatc cgcgtcgact cgctcgggca gccgcagagc
     3061 gggctggagg tacggcggga tgcacgtccg ggatacgcgg tgcggctgac gctcgacgcc
     3121 aggctgcagc gggcggcgga acgcgcgctc gtgtatggga tcgagaccgc caaggagaac
     3181 gagtcgtact tcgccaacgg cggcgcgatc gtcgcgctcg acccccgcga cggcgccgtc
     3241 ctcgcgatgg cgtcgtaccc gaccttcaag ccgtcggtgt gggtggggac gatcgacgag
     3301 aagaagatcg cgccgctgat caaggagaaa gcggcgcgcg aggccaacta cccgggggtc
     3361 aacagggcga cgagcgggct ctacccgccc ggctcgacgt ggaagccggt cgttgcgctg
     3421 gccgcgatgc aggagcacat gctctcgccg ttcgactcga ttcagtgcac gcccaccgcg
     3481 acgtacggcc tggacgagca ggagttccgc aactggaacc cctacgtcaa caagccgatg
     3541 acgctccccg tggcgctcgc cgagtcgtgc gacacgtact tctacgagat cgggaacagg
     3601 ttttacgagc gaggcctctc cgagcgcgcg ttctggacgc ggctccagga ctgggcgggc
     3661 cgcttcggtt tcggggcgtc gtcggggctc gacatcgggg gcgaggccgt ggggctgctg
     3721 cccacgccgc tctggcgaaa gcagacgttc aagagcgact gggaccgagc ctggaacccg
     3781 ggtgacctga tccagttcgc gatcggccaa aaggatttga ccgtgacgcc cctgcagatg
     3841 gcgcgctttt acgcgatgat cgcgaacggc gggaagctcg tgacgccgta catcgtgtcg
     3901 tcggtggaac agccggagac ggagggccag gggccggtgg tgctccgaag cttcgcggca
     3961 gaggccccgc gctccgccgg cgtcgacccg ggtgcgctca ccgtcgtgcg ggagggtctc
     4021 tatgccgcga cgcattcgcg ggacggaacc tcttcgggtg tcttcgcgaa ctaccccgtg
     4081 ccgatcgcgg gaaagaccgg gacggcagag aaagtcgtgc ccctccccgg ctatccggca
     4141 gatcacctcg aggatcagtc gtggtggtgt ggctgggggc cgtacaacct ggagagctac
     4201 aacgacaaat cgccgctcgt cgtctgtgcc ctgatcgaga acggtgggca cggctcgacg
     4261 gcagcggctc cggcggcgct gaagctgttc gagacctggt tcggcgtcga ggccggcgag
     4321 cagattttgg tggcgaccga ctagggcttc gtgatgaccg actatgcagg acatacgagc
     4381 tcgcgtccgc gcatccggcg ccggccggag gccgccggcg tgggggcgtt cgtgcggcga
     4441 ctggactggg tgctcgtgct ggccgtcggg gcgctcgtcg cctacgggtt gtgggcggtc
     4501 gacggcatca cgcggttcga cgtcgccggt gacccggact actacgtcgt acgtcaggcg
     4561 gtcgcggccg ggatcggtct cgcggccttg ctggtcgcat tgctcacccc gccggctctg
     4621 ttgcgccgcc actggcgcgc gctctacggg ctgaccgtgg tgctgatggt gctcgtcttc
     4681 gtcctcgccg agacggtccg cggcgcgaag aggtggatcg acgtggggcc gttccagttc
     4741 cagccgtccg agttcggcaa ggtactgctc gtcctcgcgc ttgccggctt cctcgtcgag
     4801 cgagcgtcgc gcatcaccga tgtcagaacc gtggccacgg cgatcgcgct ggcggcccta
     4861 ccaatcgcgc tcgtcttcct gcagcccgat ctgggcaccg cgatggtgta cacggccgcc
     4921 ctcgcttccg ttctcttcat cgcgggcgtt cgctggccgc acctcgcgat actcctggcg
     4981 ctggcagccc tggtggcggt gagcgtggtc tggcttctgc cgtcggcggg cgtggaggtt
     5041 ctcaagccgt atcagaccgc gcgcctgacg gggttcgcca atcccgatac cgatccagga
     5101 gggctgacct acaacgtgac gcagtccatg accgcgatcg gggctggcgg cctcgacgga
     5161 cgtggggtca cgggtgcgag ccagacgcgc ctggcctacc tgcccgagca cgccaccgac
     5221 ttcgtcttcg cgtcgctcgc ggagcagcgg ggcttcttcg gcgcggcgat cctgctcctc
     5281 ctctatctcc tcgtcgtgtg gcgggggctg cgcatcgtga cgctggccgg agacctcttc
     5341 agcgccatcg tcgccggcgg catagtgttc gctttcctct tccagatctt cgtgaacgtt
     5401 ggcatgacga tgggcatcgc accggtcacc ggcattccgc tcccgttcgt caccgtgggc
     5461 ggctctgcgc tggttgcgaa tctgatcgtg atgggcctgc tgctctcgat acacgcccgc
     5521 ggtcgcatgc cgaatgggag gcgctgatag ctccggtctc cgtcctgggt gtcgtgcgcg
     5581 cgctgcgcga gagtcgcgag aacgcggaga tcgagcggac gcttcttgtg gtcggagtga
     5641 tggccgacgt tctcgcacgt gagctcacga gcgggggaga cgagtcactg gcgcgcaccg
     5701 gcggcgcgct cgacgacgcg gccggcgtcg tggtcgtgct ctccggtgcc ccgagcgatg
     5761 gcgaggtcga ccttctccgg caggcccagc gtgccggggc ggcgacggtc gtcgttcagc
     5821 ttcaacccgg cccaatggac gacatcccct atgtgctcgc agtcgacgtc gtcgacgttc
     5881 cgcccggcgc cggctttccg gtcgacgagg tcgtgcgggc gctgacccgg agaatggggg
     5941 agcacgtcgt gccgatcgcg aaggcgttgc cgcgcttccg cgcacccgcg gccagggagt
     6001 tgacacgctc gatcgccacc agaaacgcaa cgctcgcctt cgagccctgg ggatcgagga
     6061 tccatctgcc catactgctt gccaatcagg tccgactcgc cttcgacatc gcagcggcgc
     6121 acgggcgcga gatcggggca actcggctgc cggatgcggc aaccacgctg ggcctggccg
     6181 tcggcctccg tgcggcctcg cgtcgggcga gacagcacgt tccgggccgc cgactggtca
     6241 acaccgcagt cgcctacggc ggtacgcgcg ccgtcggcga gcttgccacg ctccgcgcgc
     6301 gcttcgggcc gtagccgacg cccgggtccg tccggtcctg gtcctagcat cgccctcgac
     6361 tccgagaccg agggaggcca ggtggccgaa agggagctcg cggaagagct gctgcagatc
     6421 gaagaggctg atgcatggtt cgaatacctc gaggcgaccc ggggtcagtc cgagcgtcgc
     6481 tacgccgagg tcgaaccgtg ggcgtgggca cgtctcaacc aacggctgcg tgctgtcggt
     6541 gcgcgccgag cgcgacttcg tccggcggcc gcctagccga actgatcgac tgctagcctt
     6601 ctgctcgagg ccgatggcca ctgaattcca caatcgctgc gcggcgcgga ctccgtccat
     6661 ggggtctagg cgcccgcgcg cgctgacccg aggagaactg atcgttgccc tggtttcgat
     6721 ggaagaagaa caagcaggac aagaccgtcg cggcagtccc gccggccgtg acggaggagg
     6781 taccggcccg agctgagccg gaaacggccc gagcggccgt gaccgaggcg gcacccagcg
     6841 aggccgtcgc accgaagcgc aagcgtcgcc gcggcagccg cggcgggcgc ggccggaaaa
     6901 agtcgacagc agagagtgga gcgaaaagcg cttccaccga ggcatcgacc caagaccgga
     6961 agacgagcgc cgccaagtcg aagagccggg agcccgcaaa gcgggcgacg agccgccgcc
     7021 gccaggctcc gcgccgtgct ccacttccgg ctgccaagcg agagctcgtc atctcggttg
     7081 atgcaggcga acagcgagtc gccgtgctcg aggacgaaag ggtcgcggag gtctacctcg
     7141 agcgcccgga gcgtcgttcg atcgccggca acatctacct gggtgtcgtc gacaacgtgt
     7201 tgccgggcat ggaggccgcg ttcgtcgaga tcgggctgga gaagaacggt ttcctctacg
     7261 tcgacgagat cgtggttccg gagctcgaag gcaagcgtca cggaaagcgc atccaggacc
     7321 tgatctcgcg cggcgaggag atcctcgtgc aggccgtgaa ggatccgatg aagacgaagg
     7381 gggcgcggct cacgaccgag atctcgctgc ccggtcggtt tctcgtcttc gtcccgaacg
     7441 gcgaggggct cggagtatcg cgtcgcctcg aggacgccga gcgccaacgc ttgaaggaga
     7501 tcatcaagga gatcgtgccg aaggacggcg gggtcatcgt gcgcacagcg gccgacggcg
     7561 catcggcgga ggacatcgag cgcgacctgg tcttcctcga gcgactctgg aagcacatcc
     7621 aggcgcgcat gaaggagtcg aaggcgccag cactcgtcta ccgagaggcc gagcttccgc
     7681 tccgcatcgt ccgcgacctg ttcgccggcg acttcgtggc tgcgacgatc gaccacgagc
     7741 gcacgttcaa gcggatcgtc ggctacctga agaagacgtc gccgcacatg ctcgagcgcg
     7801 tccaccggta caaggaaaag acgccgctct tcgaggcgca cggaatcgac gcggagatcg
     7861 agtcgacgct cgaccggcgg gtgaacctgc cctcgggcgg ttacctcatc ttcgactacg
     7921 cggaggcgtt caccgtcatc gacgtgaaca cgggtcgctt cgtcggctcg cggtcgaaga
     7981 gctcgaccgc gcgtctcgag gacacgatca ccaagaacaa cctcgaggca gtcaaggagg
     8041 tagtccggca gctccggctg cgcgacatcg gcgggatcat cgtcatcgac ttcatcgaca
     8101 tggccaaccc gaagaaccgg gcgtcggtgg aggaagcact acgcagcgag ctggagcgcg
     8161 atcggacgaa gacgtacgtg gtggagatct cgcccctcgg tctcgtcgag atgacgcggc
     8221 agaaggtcac ggaggggccg cgggagatcc tgacgcgtaa atgcgaggtc tgtgcgggcg
     8281 acggggtcgt cgtctctgat cagacggtgg cgatcgaggt ggagcgcaag ctgcgtgctc
     8341 tcgccgcggg cggggcacgg gttcaggcgt accgagtcgc cgtgcatccc cgcgtccgcg
     8401 ccttggtcgc cggtccgggt ggccgccggt tggcggagat cgaggaggtc gcgaggcgcc
     8461 gtttctatct cgaggagccc gacaacggtc acgtccacat cgatcacttc gacgtgctcg
     8521 ctcagggaaa gcgtgacgtc ctggcgccgg acggcgacgt tcaggaagac gtcgagatga
     8581 gcgtcaagct caccgaggtc gaccgcttcg actcctcctc cgcagtcggc agggtgggcg
     8641 acgtcgagat cgtcgtggct gacgcggcgt cgctggtcgg taagagcgca aaggtcgttc
     8701 cgggacgcgt ccttgccgga gcggcatacg ccgtgctcgc cgacgcccag gacggcgacg
     8761 gctcgatcac cttcgagagc gaggcggaga agccaacccg ggcccccgcc aagacgaagc
     8821 gatcgtcggc cgtgaagaag gaaaccgacg cgggaggcga cgagaagtcg acgccgcggc
     8881 cgaagccggc aaagacggcc aaggcgaagg aaaagcccgc tcggcagaaa aaggtcgccg
     8941 agcccgcgac gggcgacgag gcggcttccg ccggcgagga gccagaagcc acggacgccg
     9001 accgcgcgga cggcaaggcc tcccaggcga cgactccgcg gaagcggacg cgtcgtggct
     9061 cgcgaggcgg gcgccgccgc cgcaagcccg cgacgtccgg agctgccgcc gccgacggcg
     9121 cggccggcga cggaaaggcc gaggcggagg gcgcggccga ggcggccaag gggaagacgc
     9181 ccaagcgagc gcccaagatc cacgtgccgg atgcggctcc ggccgagaac ggcggcaccc
     9241 cggccgaaac ggcttcggca gagtc
//
LOCUS       DBNGNK010000051         2992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_797201,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000051 DBNGNK010000000
VERSION     DBNGNK010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_797201"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="AC150V_01340"
     CDS             complement(<1..701)
                     /locus_tag="AC150V_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3761598.1"
                     /translation="MSPLPPPVRYTKAGSANIAYQIAGDGPVDLVLALGAWTHLELAW
                     EIPPLRRFLERLSSVSRLIFFDKRGTGLSDRLAPEETPTLAERMEDIVGVMDAAQSER
                     AVLFGTLGASAIAGNYAVMHPERTLGLVVYGAYPKIGPGTGMLSRIADDAERALDRVE
                     REWGTEAVTLAFWAPSVVTNEELKSAHLHLARSALSPGAARTMMELGFRADWEAIMPS
                     IRVPTLVLHRSGDLAV"
     gene            753..1640
                     /gene="dapA"
                     /locus_tag="AC150V_01345"
     CDS             753..1640
                     /gene="dapA"
                     /locus_tag="AC150V_01345"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR3761599.1"
                     /translation="MLGEVLTAIVTPFDDKGEVDLGSFRALARHLVDNGSDGLVVTGT
                     TGEAPTLSDEERMALYAAAVEEVGDGATVIAGTGTYATEHSVHLTKEADALGVDGFLV
                     VTPYYNKPPQRGIVAHFEAIAAVTDKPIVVYNIPGRVVINIEPETISRLAEIANVRAV
                     KQAKPETDEARHIVETGLDLYAGDDDLILPFLELGGTGGVCVHTHVVGPQVKELIGLY
                     KRGDVDGARAVDRELVPSIELLRAATNPIAIKCALNLLGHDVGGLRLPLVEADEEETA
                     TVRDCLARLGQLDRAAAQP"
     gene            1651..>2992
                     /locus_tag="AC150V_01350"
     CDS             1651..>2992
                     /locus_tag="AC150V_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3761600.1"
                     /translation="MAACPSCGSENREGARFCDACGSALEAAPAAESKEERKVVSVLF
                     CDLVGFTAASESADPEDVRARLRPYHERLRERIEAFGGTVEKFVGDAVMAVFGAPVAH
                     EDDAERAVRAGLSILEALEGMNEDDPGLSLSVRIGVNTGEALVALDARPETGEGFVTG
                     DVVNTAARIQSVAPVGGVAVGEGTHGATEPVFEWEELPAAEAKGKSEPVRLWRALAAR
                     ARFGSDVIRRLSTPLVGREADMLQLRAAFEKSSREPSVQLVTVVGEPGVGKTRLVAEL
                     TAHVDALPDLVRWRQGRCLPYGDGIAFWALGEIVKAQLGVYESDSQTDAEAKLDRLLP
                     AAEDTPWLKARLLPLLGIDPGVTATREESFTAWRRFVESIAENEPAVLVFEDLHWADE
                     ALLDFLEYLADWAQGVPLLLLCSARPELLERRPTWAAGLRNATTIGLAPLSGAEI"
BASE COUNT          475 a          982 c         1083 g          452 t
ORIGIN      
        1 acggccaggt cgccggaacg gtgaagcaca agtgtcggca cccgaatcga gggcatgatc
       61 gcctcccagt cggcccggaa gcccagctcc atcatcgtgc gtgcggctcc ggggctgagc
      121 gcagagcggg cgaggtgaag gtgcgcagac ttgagctcct cgttggtaac gacactcggt
      181 gcccagaacg cgagagtcac ggcctctgtg ccccactccc gctcgacccg gtcgagagcg
      241 cgctcggcat cgtcggcgat acgcgagagc attcccgttc cgggcccgat tttcggatag
      301 gcgccgtaga caacgagccc gagggtgcgc tccggatgca tgacggcgta gttgcccgcg
      361 atcgcactgg cgccgagcgt cccgaagaga actgcccgct ccgactgagc ggcatccatc
      421 acccctacga tgtcctccat tctctcggcc agcgtcggcg tctcctccgg tgcaagtcga
      481 tccgacagac cggtgccgcg cttgtcgaag aagatcaagc gcgagaccga cgagaggcgc
      541 tcgaggaaac ggcgcagtgg agggatctcc caagcaagct cgagatgcgt ccaggctccc
      601 agtgcgagca cgagatcgac cgggccgtcg ccggcgatct ggtaggcgat gttggccgag
      661 ccggccttcg tgtagcggac cgggggtgga agtgggctca cctaaccgca gaatgtaggt
      721 gacctcgagc tccggcgtcg tacactgtcc ccgtgctggg ggaagttctc acagccatcg
      781 tcacgccgtt cgacgacaaa ggcgaggtcg acctgggcag cttccgggcc ttggcgcggc
      841 atcttgtcga caacggctcc gacgggctcg tcgtcaccgg cacgacgggt gaggcgccga
      901 cgctgagcga cgaggagcgc atggcactgt acgcggcggc cgtcgaagag gtgggcgacg
      961 gcgcaacggt gatcgccggc acgggcacgt acgccacgga gcactccgtc catctcacga
     1021 aggaggccga cgcgttgggg gtcgacggct tcctcgtcgt gacgccgtac tacaacaagc
     1081 cgccgcaacg cggcatcgtc gcgcacttcg aggcgatcgc agcggtaacc gacaagccga
     1141 tcgtcgtcta caacatcccc ggcagggtcg taatcaacat cgagcccgag acgatctcgc
     1201 ggctcgccga gatcgcgaac gtcagggccg tcaagcaggc gaagccggag accgacgagg
     1261 ctcgccacat cgtcgagacc gggctcgacc tgtacgcggg cgacgacgac ctcatcctcc
     1321 cgttcctgga gctcggcggc acgggcggcg tctgtgtcca cacgcacgtc gtcggcccgc
     1381 aggtgaagga gctgatcggg ctctacaagc ggggcgacgt cgacggggcg cgcgccgtcg
     1441 accgggagct cgtcccgtcg atcgagctgc tccgtgcggc cacgaacccg attgcgatca
     1501 aatgcgcgct caacctgctc ggccacgacg tcggtggcct gcgtctgccg ctcgtcgagg
     1561 cggacgagga ggagacggcc accgtacgcg actgcctcgc acgtctcggc cagctcgatc
     1621 gcgccgccgc ccagccgtag gatcgtcaac atggcggcct gcccgtcttg cggcagcgag
     1681 aaccgagaag gggcgcggtt ctgcgacgcg tgcggcagcg cgctcgaggc tgcgcctgcg
     1741 gcggagagca aagaggagcg caaggtggtg tcggtgctct tctgcgacct cgtcggcttt
     1801 accgcggcgt cggagtcggc agatccggag gacgtgcggg cgaggttgcg gccgtaccac
     1861 gagcggctgc gggagcggat cgaggcgttc ggggggacgg tggagaagtt cgtcggcgat
     1921 gcggtgatgg ccgtgttcgg agcgccggtt gcgcacgagg acgacgcaga gcgcgccgtg
     1981 cgcgctggtc tctcgatcct cgaggcgctg gagggcatga acgaggacga cccggggctc
     2041 tcgctcagcg tgcggatcgg cgtcaacacg ggcgaggcgc tcgtcgccct cgacgcgcgt
     2101 cccgagaccg gcgagggctt cgtcaccggc gacgtggtca acacggccgc gcgcattcag
     2161 tcggttgctc ctgtcggtgg tgtcgcggtc ggggagggga cgcacggcgc cacggagccc
     2221 gttttcgagt gggaggagct gcctgcggcc gaggccaagg gcaagagcga gccggtgcgg
     2281 ctgtggcggg cgctggcggc gcgggcccgc ttcggctcgg acgtgatccg ccgtctctcg
     2341 actccgttgg tagggcggga ggccgacatg ctgcagctgc gggccgcgtt cgagaagagc
     2401 tcccgtgagc cgtccgtcca actcgtaacc gtcgtcggtg agccgggagt ggggaagact
     2461 cggctcgttg ccgagctgac ggcgcacgtc gatgcgcttc ccgacctcgt gcgctggcgc
     2521 cagggtcgct gcctgcccta cggcgacggc atcgcgttct gggcgctggg cgagatcgtg
     2581 aaggcgcagc tcggcgtcta cgagtccgac tcgcagactg acgctgaagc gaagctcgac
     2641 cgtctgctcc cggcagcgga ggacacgcca tggctcaaag cgcgtctgct gccgctgctc
     2701 ggcatcgacc cgggcgtgac ggcgacccgc gaggagtcct tcaccgcttg gcggcgcttc
     2761 gtcgagtcga tcgccgagaa cgagcccgct gtgctcgtct tcgaggacct gcactgggca
     2821 gacgaggcgc tcctcgactt cctcgagtac ctggccgact gggcgcaggg ggtgccactg
     2881 ctcctgctct gcagcgcgcg accggagctg ttggagcggc gcccgacctg ggcggcaggt
     2941 ctccgcaacg cgaccacgat cggtctcgct ccgctctcgg gcgccgagat cg
//
LOCUS       DBNGNK010000052         2999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_510050,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000052 DBNGNK010000000
VERSION     DBNGNK010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_510050"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="AC150V_01355"
     CDS             <1..393
                     /locus_tag="AC150V_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003923271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HIR3761601.1"
                     /translation="EGTMHNRARLVTASFLTKHLYVDWREGAAHFAAHLVDGDVANNN
                     LNWQWVAGTGADTRPNRVFNPTRQAQRYDPSGEYVRRWIPELRSLEGASVHEPWRAGL
                     LEPSDYPAPIVEHEEAVERFRAARSGRT"
     gene            390..845
                     /locus_tag="AC150V_01360"
     CDS             390..845
                     /locus_tag="AC150V_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3761602.1"
                     /translation="MSAPLFELERIQVVPVDRARAFAFFGDPWNLEAITPPWLRFRIT
                     DAPAQLGRGSRLAYALRLDGFPVRWLTEIADWHPDRSFTDVQLRGPYRSWVHTHRFSD
                     VAGGTEIYDHVAYRVPGGQLAPLLHRLVVAPRLEEIFAYRAAAIGELFR"
     gene            complement(842..1705)
                     /locus_tag="AC150V_01365"
     CDS             complement(842..1705)
                     /locus_tag="AC150V_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR3761603.1"
                     /translation="MNVAVLGASGVIGTALLPELAKQHRVVAVARRPRPSTESVTWVA
                     ADVTDAAGMDDVLRGVDVVVYLVHSLGSGDFAARDRQAALTVALASERAGVRQIVYLG
                     GLGEDDPDLSEHLKSRQETARLLASGSVPVTTLRAAMVVGAGSAAFETIRALVDRLPA
                     MICPRWVSVPTQPIALSDIVKYIAGVCGREEAYGETFDVGGPEIMTYRQMIERIAAVR
                     DKHPLIVEVPVLTPRLSSYWLHLVTPVNAAVARPLIEGLRNPTVAHETRIREIVPVEL
                     TSFESAVRAAS"
     gene            complement(1702..2202)
                     /locus_tag="AC150V_01370"
     CDS             complement(1702..2202)
                     /locus_tag="AC150V_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761604.1"
                     /translation="MAESPDTWPGDLDERGVFESLQDADLAAPLVAATDAGALELGER
                     YWNEVETLTHGVLRVNENEAGTDILLAGRVPLLRVSPPRAAVADDHIACVYEIRGGLL
                     AARPGGTLSLEQRSGRRPALAVVVAGYRPRLASVGHWLYLGLQAPVHRAVSRRFLGRA
                     VERSAP"
     gene            complement(2220..>2999)
                     /locus_tag="AC150V_01375"
     CDS             complement(2220..>2999)
                     /locus_tag="AC150V_01375"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3761605.1"
                     /translation="RRYGLFEPARTEGGFRLYSEADVQRALAMQANVGRGLSASEAAS
                     LALEEEAAGAATGAFADVVQQLDDRLEHFDEVGAQTVLDRLLSTVSLEAVLSEVLIPY
                     FRLLGERWEAGDSSIAQEHFASNVIRGRLMSLARGWGRGSGRRALLACAPGDEHDLPL
                     LMFGLALRSYGWRISYLGANTPPESLLSVVGELSPDAVVVSSSIRDALNGRADGFRAI
                     AELVPLWLAGGGLGPPAAAELGATYLAGDPVEAAATLAAGR"
BASE COUNT          474 a         1112 c          993 g          420 t
ORIGIN      
        1 gagggcacca tgcacaaccg ggcccggctc gtgaccgcgt ccttcctcac caagcacctc
       61 tacgtggact ggcgcgaagg ggccgcccat ttcgctgccc acctcgtcga cggcgacgtc
      121 gccaacaaca acctcaactg gcagtgggtg gccggaaccg gcgccgacac gcgccccaac
      181 cgcgtcttca acccgacgcg ccaggcgcag cgctacgacc cgagcggcga gtacgtccgc
      241 cggtggatac ctgaactccg gtcgctcgaa ggcgcgtcgg ttcacgagcc gtggcgcgcc
      301 ggactgctcg agccgagcga ctatccggcc ccgatcgtcg agcacgagga ggcggtcgag
      361 cgattccgcg cggcccgctc gggccgcaca tgagcgcacc gctgttcgag ctggagcgta
      421 ttcaggtcgt accggtcgac cgcgcccggg cgttcgcctt cttcggcgac ccgtggaacc
      481 tcgaggccat tacgccgccg tggttgcgct ttcgcatcac cgacgcaccg gcgcagctcg
      541 gccgcggctc gaggctcgcc tacgcccttc gtctcgacgg cttccccgta cgctggctga
      601 ctgagatcgc cgactggcac cccgaccgct ccttcaccga cgtgcagctc cgcggcccct
      661 atcgatcctg ggtgcacacc catcgcttca gcgacgttgc cggcggcacg gagatctacg
      721 accacgtcgc ctaccgcgtt ccgggtgggc agctcgcccc gcttctccac cgactcgtag
      781 tcgcgccgag gctcgaggag atcttcgcct accgcgccgc cgcgatcggc gagctctttc
      841 gctagctcgc cgcccgtaca gcgctctcga aggaggtcag ctctacaggc acgatctcgc
      901 ggattcgcgt ctcgtgagcc acagtcggat tccgcaggcc ctcgatcagc ggtcgcgcca
      961 cggccgcatt cacgggcgtg acgaggtgga gccagtacga cgacagccgc ggcgtgagaa
     1021 caggcacctc gacgatgagc gggtgcttgt cgcgcacggc cgcgatccgc tcgatcatct
     1081 gccggtaggt catgatctcc gggccgccga catcgaaggt ctctccgtac gcctcctccc
     1141 gcccgcagac gcccgcgatg tacttgacga tgtcggacag cgcgatcggc tgagtcggaa
     1201 ccgacaccca gcgcgggcag atcattgccg gcagacgatc gacgagggcg cgaatcgtct
     1261 cgaacgccgc gctgccggcc ccgaccacca tcgccgcacg aagcgtcgtc accggcaccg
     1321 aaccggaggc cagaagtcgc gccgtctcct gtcggctctt cagatgctcg gacaggtcag
     1381 ggtcgtcctc gccgaggccc ccgaggtaga cgatctggcg gacgccggcg cgctcggagg
     1441 cgagggcgac ggtcagtgcc gcctgccggt cgcgtgccgc gaaatcaccc gatccgagcg
     1501 agtggacaag atagacgacc acgtcgacgc cgcgcaggac gtcgtccatc cccgccgcgt
     1561 ccgtcacgtc ggcagcaacc cacgtcacgc tctccgtgga cggccgcggc cgcctggcca
     1621 cggcaacgac gcgatgctgc ttcgcgagct ccggcaggag cgcggtgccg atcacgcccg
     1681 aggcgccgag cacggcgacg ttcatggcgc agatctttcc acggcgcgcc cgaggaagcg
     1741 gcggctgaca gcacgatgga ccggagcctg aaggccgagg tagagccagt gtccgactga
     1801 cgccagtcgg ggccggtaac cggcgacgac gacggccaga gccggccggc gcccggatcg
     1861 ctgctccaag ctgagcgtgc cccccggccg ggcggccagc aggccgccgc ggatctcgta
     1921 cacacaggcg atgtgatcgt cggcgaccgc agctcgcggc ggactgactc gcagcagagg
     1981 aacgcggccg gcgagcagga tgtccgtgcc tgcctcgttc tcgttcaccc ggagcacgcc
     2041 gtgagtcagg gtctcgacct cgttccagta gcgctcgccg agctccaggg ctcccgcgtc
     2101 cgtcgcggcg acgagcggcg cagcgagatc cgcgtcctgc agcgactcga agacgccgcg
     2161 ctcgtcgagg tcgcccggcc aggtgtcggg ggactcggcc atctcgggga cagtaatgct
     2221 cagcgccctg cggcgagcgt ggccgccgcc tcgaccgggt ctcccgcaag atacgtggct
     2281 ccgagctcgg cggcggcggg ggggcccaac ccgcctcctg ccagccagag cggcaccaac
     2341 tcggcgatcg cccggaaccc gtccgcacga ccgttgagtg cgtccctgat gctgctcgac
     2401 accacgacgg cgtcgggaga gagctctccc accacgctca acagcgactc gggcggtgta
     2461 ttggccccca ggtagctgat ccgccacccg tatgacctga gggcgagccc gaacatgagc
     2521 agcgggagat cgtgttcatc gccgggggcg cacgcgagca gcgcacgcct gccgctgcca
     2581 cggccccagc cgcgcgcaag gctcatcagc ctgccgcgga tgacgttgct ggcgaagtgc
     2641 tcctgggcga tcgacgagtc ccccgcctcc cagcgctcgc cgagcaggcg gaagtacggg
     2701 atcaagactt cgctgagcac cgcctcgagc gagacggtgg agagaagtcg gtcgagcacg
     2761 gtctgcgcgc cgacctcgtc gaagtgctcg agccggtcgt cgagctgctg cacgacgtcc
     2821 gcgaaagcgc ccgttgccgc tccggccgct tcctcctcga gggcgagact cgccgcctcc
     2881 gaggccgaca gtccgcgacc gacgttcgcc tgcatcgcaa gcgcccgctg gacgtccgcc
     2941 tccgagtaca gccggaaccc accttccgtc cgtgcgggct cgaagaggcc gtaccgtcg
//
LOCUS       DBNGNK010000053         3394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_940317,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000053 DBNGNK010000000
VERSION     DBNGNK010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_940317"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="AC150V_01380"
     CDS             <1..995
                     /locus_tag="AC150V_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3761606.1"
                     /translation="IGGVHSPELEVRALAGEAWCLSELGDQTGAIDLLLRARDIADAP
                     NFTDVDRADLMFRLGVCRYKLSSIATAIGLLDEALLLAEQSDLPCDLLRADILGWRSR
                     CRRRQRDFEAAREDVERALELAQAMDDRRVIANTYFQASLVAERMGHWIQSRSYAQQA
                     KALYQELNDERNVGRLMLNLGGLHLLLGKPEQAIEHLKASFALAIEADSKPDAAQALG
                     GLATVHLHLGDYESAETEARKALDMLDGREDFLHEIGPSSLVLGRALMETGRLAEAEE
                     CFLAADAAAEQLQSVSHRAGAWVALGDLAGRRGDDREAARHYRNAAEALQDIRF"
     gene            1137..2060
                     /locus_tag="AC150V_01385"
     CDS             1137..2060
                     /locus_tag="AC150V_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761607.1"
                     /translation="MRSAAAFLALVCILLAGCGGGAKSDAADGDNDTTTAVEESLESL
                     WRAPGEDVAVVPGTSDYGPGRNRISFLIVDSQSRLITSPTANVWVARALEAAPFQQTT
                     ARLERIGVPGGAEADASHLYVAHVDLPEPGKYWFVAEPIDADTEIQALGNLVVPKKSI
                     APAVGDPAPPSKTPTLASTGGNLKVLSTAAKPDRALYRSSVAEALAADVPFVVSFATP
                     LFCQTRACGPVVEVVSAVRKSLADTDTRFINVEIYQDNDPAKGTNRWVDEWKLPTEPF
                     TFVVGRDGKVATKLEGAFSVAELSAAVKSAK"
     gene            2144..2983
                     /locus_tag="AC150V_01390"
     CDS             2144..2983
                     /locus_tag="AC150V_01390"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3761608.1"
                     /translation="MGDYVKDVLVSTDWLAANLDDSSIVVAEVDENTDLYEEGHIPGA
                     VKLHWRDDLQDQVVRDVVSKDEFERLLGERGISNDTTVILYGDKNNWFAAYAYWYLKV
                     YGHERVKLLDGGRQKWIEEGRELTTEVATPTAASYSAKDRDETIRVRRDDVLAKLHAD
                     GVALVDVRSPQEFSGEVMAPPGYEQEGASRTGHIPGAQSIPWATAVRDDGTFKTADAL
                     SEIYAGKGVTSDKEVLAYCRIGERSAHTWFVLRELLGYGDVKNYDGSWTEWGNLVDVP
                     IEK"
     gene            complement(2995..>3394)
                     /locus_tag="AC150V_01395"
     CDS             complement(2995..>3394)
                     /locus_tag="AC150V_01395"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761609.1"
                     /translation="KHWDLQQAVNLRGAFLVTREALPLLRAAQGYVVNLSSIAGTIPS
                     PGLAGYGAAKAALIALTRSLVREEADNGVRVTALCPAFVDTPMAAWTGIPGEEMIQPE
                     DCAEILRALLRLSPRARVPVVVIERAGSEV"
BASE COUNT          602 a         1123 c         1150 g          519 t
ORIGIN      
        1 cgatcggcgg cgtccactcg cccgagctcg aagtgcgtgc gctcgcgggc gaggcgtggt
       61 gcctctccga gctgggcgac cagaccggcg cgatcgacct cctgctgcgc gcgcgtgaca
      121 tcgccgacgc accgaatttc accgacgtcg accgcgcgga cctgatgttc cgcctcggcg
      181 tctgccgcta caagctctcg agcatcgcaa ccgcgatcgg actcctcgac gaggcgctcc
      241 tgctggccga gcagtccgac cttccctgcg acctcttgcg cgccgacatc ctcggctggc
      301 gctctcgctg ccggcgacgg cagcgtgatt tcgaggccgc ccgggaggac gtcgagcgcg
      361 cgctcgagct cgcccaggcg atggacgatc gccgggtgat cgcaaacacg tatttccaag
      421 cttcgctcgt cgcagagcgg atggggcact ggatccagtc gcgcagctat gcgcaacagg
      481 cgaaggcgct ctaccaggag ctcaacgacg agcgaaacgt aggccgcttg atgctcaacc
      541 tgggcggtct acacctcctc ctcggaaagc ccgagcaggc catcgagcat ctcaaggcgt
      601 cgttcgcgct cgcgatcgaa gccgattcga agccggacgc cgcacaggcg ctcggcggtc
      661 tcgccacggt gcacctgcac ctgggggact acgagtcggc cgagacggag gcgcggaagg
      721 cgctggacat gctcgacggg cgagaggact tcctgcacga gatcggccca tcgagcctcg
      781 tactggggcg agcgctcatg gaaacgggtc ggcttgcgga agcggaggag tgcttcctgg
      841 ctgcggacgc tgctgcagag cagctccagt ccgtcagcca tagggcgggg gcgtgggtcg
      901 cgctgggtga ccttgcaggt cgtcgtgggg acgaccgcga ggcagcgcgc cactaccgga
      961 acgccgctga ggcgcttcaa gacatccgct tctaagagag gaggccaaca ccatgaaggc
     1021 acggcttctg gtcatgttga ccacgcttgc gctcttcgcc gcgatggccc agcccgtctg
     1081 ggcgtcgtgg tcggacggcc actagtccac gagagctctg cggctacagt gacttagtgc
     1141 gctcggccgc ggcgtttctc gcactcgttt gtatcctgct cgccggctgc ggcggcggcg
     1201 cgaagtccga cgccgcagac ggcgacaacg acacgacgac ggccgtcgag gaaagtctcg
     1261 agtcgctatg gcgggcgccg ggcgaagacg tcgccgtcgt tccggggacg tccgactacg
     1321 ggcccggccg gaaccgcatc tccttcttga tcgtcgactc gcagtcgcgc ctgatcacgt
     1381 cgccgacggc gaacgtctgg gttgcacgcg ccctcgaagc ggcgccgttc cagcagacca
     1441 cggcacggct ggagcggatc ggcgtccccg gcggcgccga agccgacgcg tctcatctgt
     1501 atgtggccca cgtcgacctc cccgagcccg ggaagtactg gttcgtcgcc gagccgatcg
     1561 acgcagacac cgagatccag gcgctcggca acctggtcgt gccgaagaag agcatcgcac
     1621 cggccgtcgg cgaccctgca cctccttcga aaacgccgac gctcgcgagc accggcggga
     1681 acctgaaggt cctctcgacc gccgcgaagc ccgaccgcgc tctctaccgg tcgtcggtcg
     1741 ccgaggcgct cgcagcggac gttccgttcg tggtctcctt cgcaacgccc ctgttctgcc
     1801 agacgcgggc ctgcggtccg gtcgtcgagg tcgttagcgc ggtgcggaag tccttggcgg
     1861 acacggacac ccgcttcatc aacgtcgaga tctaccagga caacgaccct gccaagggaa
     1921 cgaaccgctg ggtcgacgag tggaagctcc ccaccgagcc gttcacgttc gtcgtcgggc
     1981 gtgacggcaa ggtcgcgacg aagctcgagg gcgcgttctc cgtcgccgag ctctccgccg
     2041 cagtcaagag tgcgaagtag ccgcttggcc agagcccctg agcggtggta cgatcgcggc
     2101 cgcaagccat tactatcaat acaaaagtat taggagtcgt gcgatgggtg actacgtcaa
     2161 ggacgttctc gtcagtaccg attggctagc cgccaacctc gacgacagca gcatcgtcgt
     2221 ggccgaggtg gacgagaaca cggacctgta cgaggagggg cacatccccg gtgccgtgaa
     2281 gctgcactgg cgtgacgacc tccaagacca ggtcgtccgc gatgtcgtca gcaaggacga
     2341 gttcgagcgc ctgctcggcg agcgcggtat ctcgaacgac acgaccgtca tcctctacgg
     2401 ggacaagaac aactggttcg cggcctacgc ctactggtac ctcaaggtgt acgggcacga
     2461 gcgggtgaag ctactcgacg gcggcaggca gaagtggatc gaggagggcc gcgagctgac
     2521 caccgaggtg gcgacgccca ccgccgcgtc gtactcggcc aaagatcgcg acgagacgat
     2581 ccgcgtgcgg cgcgacgacg tgcttgcaaa gctgcacgcc gacggcgtag cactcgtcga
     2641 cgtacgcagc cctcaggagt tcagcggtga ggtgatggcg ccgcccgggt acgagcagga
     2701 aggagcgtcc cgaacaggtc atatcccggg ggcgcagtcg attccctggg cgacggccgt
     2761 gcgggacgac gggacgttca agacggcgga cgctctcagc gagatctatg cggggaaagg
     2821 cgtgaccagc gacaaggaag tcctggcgta ttgccggatc ggtgagcgca gcgcgcacac
     2881 gtggttcgtc ttgcgagagc tgctcgggta cggggacgtc aagaactacg acggctcgtg
     2941 gaccgagtgg ggcaacctcg tcgacgtccc gatcgagaag tagtacgcgc cgcctcagac
     3001 ctcgcttccc gcacgttcga tcacgaccac ggggacccgc gcgcgcgggc tcagtcgcag
     3061 gagcgcgcgc aggatctcgg cacagtcctc gggctggatc atctcctcgc cgggaatgcc
     3121 tgtccacgcc gccatcggcg tgtcgacaaa ggcgggacag agcgcagtga cacgaacacc
     3181 gttgtctgct tcttcgcgta ccagcgaccg cgtcaaggcg atcagcgcgg ccttggcggc
     3241 gccgtagccg gccaggcccg gcgacggaat cgtccccgcg atcgaggaga gattgacgac
     3301 atacccctgg gccgctcgaa gcagggggag ggcctcgcgc gtgacgagga acgcgccgcg
     3361 gagattcacg gcctgctgca gatcccaatg cttc
//
LOCUS       DBNGNK010000054         7327 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1513493,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000054 DBNGNK010000000
VERSION     DBNGNK010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1513493"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1091
                     /locus_tag="AC150V_01400"
     CDS             111..1091
                     /locus_tag="AC150V_01400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012183913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR3761610.1"
                     /translation="MKAIVYRKYGSPDVLELREIDRPPVDEDQVLVRVRAASANPLDW
                     HFLRGEPYFMRITSGLRAPKVTGLGADVAGEVEAAGGTVTRFRPGDEVFGDAAGCGSG
                     TFAEYVSVREDWLEQKTADLTFEQAAAVPLAGLTALQGLRDHGRLEPGQKVLIIGAGG
                     GVGTFAVQIAKWLEAEVTGVCSTTKAELVRSLGADHVVDYTREDFTTKGELYDIVLQL
                     AGTQSPSRLRRVLESKGTLLLSSGESEGRVIGPVDRIVKALVSSPFVSQRMVPFLAKK
                     SADDLRLLKELLEAGTVTPVIDRTYPLSETPAAIRYLEEGHAQGKIVITV"
     gene            complement(1186..1692)
                     /locus_tag="AC150V_01405"
     CDS             complement(1186..1692)
                     /locus_tag="AC150V_01405"
                     /inference="COORDINATES: protein motif:HMM:NF019095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HIR3761611.1"
                     /translation="MRKLSGAVALTLVAVTAMLVLVAAGAADNGGRTLKANLTGAEEV
                     PPVVTDTTGTAKFKVDKGQTTIRFTLKIKNASDILGAAGAHIHCAPAGANGPVVAFLA
                     GAVPGGLDGKVEIKATLSGANIVNPACGATIAELVQAMRDGQTYVNVHSSGNPGGEVR
                     GQIGGDDD"
     gene            complement(1848..2768)
                     /locus_tag="AC150V_01410"
     CDS             complement(1848..2768)
                     /locus_tag="AC150V_01410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR3761612.1"
                     /translation="MRRFLAGSIVVLSAAVAGGLLDGVLWPGTLLALFRPQFTLVLLA
                     VALLALIIGPRLLALAGLTAVGLGVALLVPALRDADPDPPPAAGTRATVLALNLWRRN
                     DDVDAVVELIEKERPDVVALTELTPEWARALAPILKSFPIRAVESDEGTAGIGVYARD
                     HLRDPEVVRLAGRSRPAVQGLLELDGRRISFLLVHPTSALLPARLGAHAEELAAIGEW
                     ARKRGPRSAVCGDLNATAWTRSLREALEAGGLVPVLPGGLFAGSWAAFLPPPLRVAVD
                     GCLVGTADRARVELGPRVGSDHLPLLVELA"
     gene            3072..4019
                     /locus_tag="AC150V_01415"
     CDS             3072..4019
                     /locus_tag="AC150V_01415"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3761613.1"
                     /translation="MLFAGRSNRELAQRIAIQLDVELGDVELETFANGEVYCRYTESV
                     RGADLFILQSGSPPVNDHLLELLLMINAAKLASARRVTAVLPLLPYARQDKKSSPREP
                     ISARLLAELLESAGADRVLTMDLHAGQIQGFFRIPVDHMTALPLFASYFASKGLRGNN
                     IASVSPDLGRVKAARRFAEMLGADFAIVTKVRPGRERAIAAEVIGDVRGKVALVGDDM
                     IVTGGTLVAAAHALHQAGAVDVHAFATHALFTADSARTLAGGELSAIAVTDTVALDSA
                     TAPAGLSVISASVLLAETIQNVFADQSVSEIFAGENELF"
     gene            complement(4059..4529)
                     /locus_tag="AC150V_01420"
     CDS             complement(4059..4529)
                     /locus_tag="AC150V_01420"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR3761614.1"
                     /translation="MANEALTNALDDAGVSYTVLPHSHTETAADEAKALQIPPSEVAK
                     TLVVQTPAGNVRVVLPASQRVDFGKLSALVGAPRKSVHLVSEAELARQYPEFELGAVP
                     PLGGAHTDAVVVDPLLAGHASLVLEAGSHDESIRIAAADLVRVTDARVADICAD"
     gene            complement(4718..5407)
                     /locus_tag="AC150V_01425"
     CDS             complement(4718..5407)
                     /locus_tag="AC150V_01425"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015173095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761615.1"
                     /translation="MSDGRPVLEATGLVKVFGRGRTAVRAVDGVDLTASAGEITLVMG
                     PSGSGKTTLLTMVGGLLHPTEGRIEVHGTDLTRLGKGDLTRYRRHVVGFIFQTFNLLE
                     TLSTRENVEIALNVAGVNGASARERATELLVAAGLRERLDFPAHELSSGEKQRASIAR
                     ALANRPRLLLADEPTANLDSSHGHDVMILLRRLAKEEGCGVVAVSHDERLKSIADRVL
                     WLEDGRLQDER"
     gene            complement(5404..6450)
                     /locus_tag="AC150V_01430"
     CDS             complement(5404..6450)
                     /locus_tag="AC150V_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761616.1"
                     /translation="MSICGVAFAVLLILLVASLYRGWSAAGGIFAELPGDLWLAQSGA
                     SDPLRTTSFLPADGLGPLSALPGVGTVVPVFARRVSVTPGNAELNAYVMSLATPSRAA
                     WPPAARRFLPGPGAIVVDGVFAEEAGVAVGDTLELLGRALVVERVEPGGNPLFEFAFM
                     NAADARELLALDQYVSFFLITLQPDADIASVARSAVAAVAGSEVHTAAEFATATRDVV
                     NQGFLPVVGALVGIGFAIGGAVIALTIYTATVERARDFAVLKAIGADDFFVYRIVVQQ
                     SLGVGIAGATLGIVSSSLAATLIRREVPEFVTDLQPLDAAGVFAIALVVSTLAALVPV
                     RRISRIDPAMVFRA"
     gene            complement(6582..>7327)
                     /locus_tag="AC150V_01435"
     CDS             complement(6582..>7327)
                     /locus_tag="AC150V_01435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR3761617.1"
                     /translation="NPHARSGGSPMRPWGHTRDWRRHVGRERDMGPNESKLVAARSAT
                     LQVDGLGLPGILAVPAEASCVVAFAHGSGSSRLSPRNRLVASHLNEERIGTLLFDLLT
                     DEEALDLRKVFDIGLLSDRLARATRWLGLQPPTQSKPVGYFGASTGAAAALCAAAALG
                     GTVGAVVARGGRPDLAEEVLASVRAPTLLIVGGADDLVLELNRRALRRLTCEAELSVV
                     PGAGHLFEEPGALETVADLAGEWFLRHLA"
BASE COUNT         1211 a         2471 c         2535 g         1110 t
ORIGIN      
        1 agggagcgtg ccgaaggggc ttgtcggccc aaggggcgat agagtctcgc tcctcggcca
       61 gcctgagaag ctgctccgcg gccgaagccg cacgcgcgcg aaaggacgcg atgaaggcga
      121 tcgtctaccg caagtacggc tcacccgatg tgctcgagct gcgagagatc gacagacccc
      181 ccgtcgacga ggatcaggtt ctggtacgcg tccgcgcggc gtccgccaac cccttggact
      241 ggcatttcct gcgaggcgag ccgtacttca tgcgcatcac cagcggcctg cgcgcaccca
      301 aggtcacggg cctgggggcc gacgtggcag gcgaggtcga ggcggcgggc ggcaccgtca
      361 cgcggttccg tcccggcgac gaggtctttg gggacgcggc cggctgtggc tcgggcacct
      421 tcgccgagta cgtgagcgtc agggaagact ggctggaaca gaaaaccgcc gatctgacat
      481 tcgagcaggc ggcggccgtg cctctcgccg ggctcacggc gctccagggc ctgcgcgacc
      541 acggccggct cgagccgggc caaaaggtct tgatcatcgg cgccgggggc ggtgtgggca
      601 ccttcgcagt gcagatcgcc aagtggctcg aagccgaagt gaccggtgtg tgcagcacta
      661 cgaaggcgga gctggtccga tcgctcggag ccgaccacgt cgtcgactac acgcgcgagg
      721 acttcaccac gaagggggag ctctacgaca tcgtgctcca gctggcgggg acgcagtcgc
      781 cgtcgcgtct ccgacgcgtt ctcgagtcca aagggacgct cttgctgagc agcggtgaat
      841 cggagggccg cgtgatcggt cccgtcgatc gcatcgtcaa ggcgctggtg tcgtcgccgt
      901 tcgtgagcca gcggatggtc ccgtttctcg cgaagaagag cgccgacgac ctgcgcctcc
      961 tgaaagagct gctcgaagcc ggcacggtca cgcccgtgat cgaccggacg tacccgttga
     1021 gcgagacgcc cgcggcgatc cgctacctcg aagagggtca cgctcagggc aagatcgtca
     1081 tcaccgtctg accgagagcg cggccggtcg cgtcccgctg tcccgttggt ctgcgggggg
     1141 tgggctcccg ccgcgcgcag cgcgacggga gccgacagcc cgcccctagt cgtcgtcgcc
     1201 gccgatctgg ccgcgtacct cgccgccagg gttgccgctg ctgtggacgt tgacgtaggt
     1261 ctggccgtcg cgcatcgctt gcacgagctc ggcgatcgtc gcgccgcaag ctgggttgac
     1321 gatgttcgcg ccgctcaacg tcgccttgat ctcgaccttg ccgtcgagac caccggggac
     1381 tgcccctgcc aggaacgcga cgaccggccc gttcgcgccg gccggtgcgc agtggatgtg
     1441 ggcgccggcg gcgccgagga tgtccgaagc gttcttgatc ttgagcgtga acctgatcgt
     1501 cgtctggccc ttgtcgactt tgaacttcgc agtgcccgtc gtgtcggtga cgacaggcgg
     1561 cacctcctcg gcacccgtca ggttcgcctt gagcgtgcga ccaccgttgt ccgcggctcc
     1621 cgccgcgacg aggacgagca tggccgtaac cgcgacgagc gtcagggcga ctgccccgct
     1681 gagctttctc atcgtcttcc tcccacgtcg aatagagacc ggcccccgcg gaaccgagcc
     1741 ccgggcgcag cacccggcta ctagggatac cggcggcgag agcaatcgga ttcacacaaa
     1801 cgcctcccct acctcgtggc tcggcgtctg cgaaccctcc gaaaccctca cgccagctcg
     1861 acgaggaggg gcagatggtc ggagccgacg cgggggccga gctcgacgcg ggcgcggtcg
     1921 gcggtgccca caaggcagcc gtcgacggcg acgcgaagag gtggtggcag gaatgctgcc
     1981 cacgagcccg cgaagagacc gcctgggagc acgggaacaa gcccgccggc ctccagcgct
     2041 tctcggagtg accgtgtcca ggcggtggca ttcagatcac cacacaccgc acttcgcggc
     2101 ccacgctttc gcgcccactc gccgatcgcg gccagctcct ccgcgtgggc gccgagtcgg
     2161 gcgggcagaa gcgcagacgt cgggtgcacc agaaggaacg agattctccg cccgtcgagc
     2221 tcgagcagtc cctgtaccgc cggacggctg cggcccgcca gccggacgac ctccggatcg
     2281 cggaggtggt cgcgtgcgta cacgccgatc ccggccgtgc cctcgtccga ttccaccgcg
     2341 cggatcggaa acgacttcag gattggagcg agcgcccgag cccactcggg cgtcaactcg
     2401 gtgagggcga cgacgtcggg ccgctccttc tcgatcagct cgaccaccgc gtcgacgtcg
     2461 tcgtttcggc gccagaggtt gagcgccagc accgtcgctc gcgtcccggc ggccggaggc
     2521 gggtcgggat ccgcatctcg aagcgccggc acgaggagcg cgacgccgag accgacggca
     2581 gtcaacccgg cgagggcgag cagccggggg ccgatgatca gggcaaggag cgcgacggca
     2641 aggagcacga gcgtgaactg cggccgaaag agcgcgagca gagtcccagg ccagagaacg
     2701 ccgtcgagca gcccgcccgc gactgccgcc gacaagacga cgatcgatcc tgcaaggaac
     2761 cggcgcatac gaagatcgtg cacggttccg cggtctgggg ctttccacgg agtcgatcgg
     2821 attccgcaaa gcgcgcctcc ccggacaagg ccggcgcgct gatcgcgtcg gctttccact
     2881 catcgggact ccgcggcggc cacgatggcc ttatgaggct catcaacgaa gatcgcgttc
     2941 tgaggacgtc tgtcgacgga gggatcgtgc cgggacgagc tgatcggacg ccgaggaggt
     3001 ctccgctgtc ctcagggagg cgtgggcctc gccggcggcg cggccagtgg agccgaccaa
     3061 gacccaggct gatgctgttc gctgggcgct ccaatcggga gctggcgcag cggatcgcta
     3121 tacaacttga cgtcgagctc ggcgacgtcg agctcgagac gttcgccaac ggcgaggtct
     3181 actgccgtta cacggagtcg gttcggggcg ccgatctttt catcctgcag tcgggctccc
     3241 ctcccgtgaa tgaccacttg ctcgagttgc tgctgatgat caacgcagcc aagctcgcat
     3301 cggcgcgtcg cgttaccgcc gtccttccgc tcctgcccta cgcgcgtcag gacaagaagt
     3361 cgtcgccgcg cgagccgatc agcgctcgcc tactcgccga gctgctcgaa tcggccgggg
     3421 ccgaccgagt gctgacgatg gacctccacg caggacagat ccaaggcttc ttccgcatcc
     3481 cggtcgacca catgacagcg ttgcccctgt tcgcgagcta cttcgcgagc aaaggtcttc
     3541 gcgggaacaa catcgcctcg gtgtcacccg acctcggccg cgtgaaagcg gcacgacgct
     3601 tcgccgagat gctgggcgcg gacttcgcga tcgtgacgaa ggtccgcccg gggcgcgagc
     3661 gcgccatcgc agccgaggtg atcggcgacg ttcgcgggaa ggtggcgctc gtcggcgacg
     3721 acatgatcgt cactggcggg acacttgtcg ccgcggctca cgcgctccat caggcaggag
     3781 ctgtggacgt gcacgcgttc gctacacatg cgctcttcac cgctgattcg gcccgaacgc
     3841 tcgccggtgg tgagttgagc gcgatcgcgg tcaccgatac cgttgcgctc gacagcgcga
     3901 ccgcccctgc agggctatcg gtgatctccg cctcggtgtt gctcgcggag acaatccaga
     3961 atgtcttcgc cgatcagtca gtgtccgaga tcttcgcggg agagaacgag ctcttctgaa
     4021 cacaggcgtt ccgcggaaga gctggctggc cggcgcggct agtcggcgca gatgtcggcg
     4081 actcgagcgt ctgtgacgcg gacgaggtcg gctgcggcga tccggatcga ctcgtcgtgc
     4141 gagcctgcct cgagcacgag cgatgcgtgg cccgccagca atggatcgac gaccacagcg
     4201 tcggtgtgcg cgccgccgag cggcggcacg gcgccgagct cgaactccgg gtactgacgt
     4261 gcgagctccg cttcgctgac caggtggacg cttttcctcg gcgcgccgac gagcgcagac
     4321 agcttgccga agtcgacccg ctgggatgcg ggcaagacca cgcgcacgtt tcctgccggc
     4381 gtctggacga ccagggtctt cgcaacctcg ctcggcggga tctgaagcgc cttggcttcg
     4441 tccgctgccg tctccgtgtg gctgtgcggg aggactgtgt aggagacacc cgcgtcgtcg
     4501 agggcgttcg tgagggcttc attcgccact gtgagacctc ctttgcacca ctatacgcct
     4561 gcgtcatccg agctcggggc gagtgaacac ggctttggcc agccgcctgc cgctccgccc
     4621 agcgcgcggc gcgactccct cggtcgggcg agacagatca acgagcactg ctcgttgatg
     4681 tgcggacacc gcgacgagag cgcggcctcg agcgctatca gcgctcgtct tggaggcggc
     4741 cgtcttcgag ccagaggaca cggtcggcaa tgctcttgag ccgctcgtcg tgcgagacgg
     4801 caacgacccc gcaaccctcc tccttcgcaa ggcggcgcag aaggatcatc acgtcgtgtc
     4861 cgtgactcga gtcgaggttc gctgtcggct cgtcggcaag caggagacgc ggtcggttcg
     4921 cgagggcgcg ggcgatcgac gcgcgctgct tctctccgct cgagagctcg tgcgcaggga
     4981 aatcgagacg ttcgcggagg ccggcagcaa cgagaagctc ggtggcgcgc tcgcgtgcgg
     5041 acgcaccgtt gacgcccgcg acattgagtg cgatctctac gttctcccgg gtgctcagag
     5101 tctcgaggag gttgaaggtc tggaagatga agccgaccac gtggcgtcgg tagcgcgtga
     5161 ggtcgccttt gccgagcctg gtcaggtcgg tcccgtgaac ctcgatccga ccctccgtcg
     5221 gatggagtag gccgccgacc atcgtcaaga gggtcgtctt gcccgagccg gagggaccca
     5281 tcacgagcgt gatctcgccg gcagaggcgg tcaggtcgac gccgtccacc gctcggactg
     5341 ccgtccgtcc gcgtccgaag accttgacga gtccagtggc ctcgagtact gggcgtccgt
     5401 cgctcatgcc cggaacacca ttgccgggtc tatccggctg attcgccgca cagggacgag
     5461 ggccgcgagg gtggagacga cgagggcgat cgcgaagact cccgcggcgt caagtggctg
     5521 caggtcggtg acgaactccg gcacctcgcg gcgaatcagc gtcgcggcca ggctcgacga
     5581 gacaatgccg agcgtggccc ccgcgatgcc gacgccgagg ctctgctgga caacgatccg
     5641 gtagacgaag aagtcgtcgg ctccgatcgc cttgagcaca gcaaagtcgc gcgcccgctc
     5701 aacagtggcc gtatagatcg tgagggcgat caccgcgccg ccgattgcaa agccgattcc
     5761 gacgagagcg ccgacgaccg gcaggaatcc ttggttgacg acgtcgcggg tcgccgtcgc
     5821 gaactcggcg gcggtgtgca cttcgcttcc ggcaaccgca gccaccgcgg atcgtgcgac
     5881 ggaagcgatg tcggcgtccg gctggagcgt gatgagaaag aagctgacgt attgatcgag
     5941 ggcaagcagc tcgcgggcgt ctgcggcgtt catgaaggcg aactcgaaca gggggttgcc
     6001 gccgggctcg acccgttcca cgaccagcgc ccggccgagt agttcgagcg tgtctccgac
     6061 cgcgacccca gcctcctcag cgaacacgcc gtcaacgacg atcgcgccgg gcccggggag
     6121 gaatcggcgc gcggcgggcg gccaggcagc gcgcgagggg gtcgccaacg acatgacgta
     6181 ggcgttcaac tctgcgttcc cgggcgtcac ggagactcgc ctggcgaaga cggggacaac
     6241 cgtgccgacg ccgggcaggg ccgatagggg gccgagcccg tcggcaggga ggaacgacgt
     6301 cgtacggagc gggtcactgg ccccggactg cgcgagccat aagtctccgg ggagctcggc
     6361 gaagatcccg ccggccgcgc tccagccccg gtagagactc gccacgagca gtatgagcag
     6421 gacggcgaac gccacaccgc agatcgacat cagcaagcgc gccttctcag ccagcaggtt
     6481 ccggcgcgcg acagggactc ggcgcgacca ccacctcgaa gtcggtcccg gccccacgac
     6541 tccgtacccc gattgcagat caggtggggt ctcgggccgc atcaggcgag gtgccggagg
     6601 aaccactcgc cggcaaggtc tgcgaccgtc tctagtgctc cgggctcttc gaagaggtgt
     6661 ccggcgcccg ggacgacgga cagctcggcc tcgcatgtga gcctccgaag cgcgcgccgg
     6721 ttcagctcca gcacgagatc gtcggcgccg ccgacgatga ggagtgtcgg ggctctcacg
     6781 gaggccagca cctcctcagc aaggtcaggc cggccgccgc gggcgaccac cgcccccaca
     6841 gttcccccga gcgcagccgc ggcacacagt gccgcggctg cgccggtgct ggctccgaag
     6901 tagccgacgg gcttcgactg cgtcgggggc tgcagtccga gccagcgggt cgcacgcgca
     6961 aggcgatcgg aaaggaggcc gatatcgaag accttgcgca ggtcgagcgc ttcctcgtcg
     7021 gtcaagaggt cgaaaaggag tgtgccgatg cgctcctcgt tgaggtgcga cgcaaccaga
     7081 cggttccgcg gactgagccg gctggagcca cttccgtgcg cgaacgcgac gacgcacgac
     7141 gcctcggccg gcaccgccag tatgcccggt aaccccagac cgtccacctg cagcgtggcg
     7201 ctgcgcgccg caaccagctt ggactcgttc ggccccatgt cacgctccct gccgacgtgc
     7261 cgcctccagt ctcgcgtgtg gccccagggt ctcatcggag agcccccgga tctcgcgtgg
     7321 gggtttt
//
LOCUS       DBNGNK010000055         4802 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_367294,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000055 DBNGNK010000000
VERSION     DBNGNK010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4802)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_367294"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC150V_01440"
     CDS             <1..279
                     /locus_tag="AC150V_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761618.1"
                     /translation="YAKVVVKVETGFCVANNITFPSTVYEGAVGALSFTNAAHVPSSG
                     GTLPVNTTHCYRFSFKLADDAGNALQGKSATFNVIIDATQPENPGWSE"
     gene            337..870
                     /locus_tag="AC150V_01445"
     CDS             337..870
                     /locus_tag="AC150V_01445"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3761619.1"
                     /translation="MARRAQQALDRMAVLSTGRPVEKPNGRPRDLRRVASAATSVLLA
                     VGLLFAVFVVYGSAIENRWYKIVAIEGGSMEPTMHAGDAIVVSRPPKTLEKGMVVLLQ
                     INDKLVTHRVLAVKPDGSFTTKGDANDYADDWTGTGVRVAGVYRAKIPLLGRALSLGS
                     GAWLVDRAQTPVTATGG"
     gene            complement(1182..1255)
                     /locus_tag="AC150V_01450"
     tRNA            complement(1182..1255)
                     /locus_tag="AC150V_01450"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1219..1221),aa:Asp,seq:gtc)
     gene            1343..2476
                     /locus_tag="AC150V_01455"
     CDS             1343..2476
                     /locus_tag="AC150V_01455"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HIR3761620.1"
                     /translation="MTVLDLVLLGVLALMALGGFRRGLLVGVLSLAGLVAGAYVGARV
                     APGLLDDGSRWIPVATLVGAVVAAALGQWGAVTAGRSLRALVTIGPLSALDRVGGAVL
                     GIATGLVFCWVLGAVLLYLPGQTELRRYAQESAILSTLNQEFPPHRLMDALSRVDPFA
                     SLAGPAANVAPPEPAIAGDPDVIAARLGVVRVTGFACGLGVEGSGWIAGPELVVTNAH
                     VVAGVDVPRVDQGDPRLYQGAVVHFDARNDLALIRVPGLRGAQLQLGDPERGLAVAML
                     GFPQNGPYQATPARLGATVTSVGRDAYGRFPVPRSVTTIRGDIVSGNSGGPAVDADGR
                     VRTTIFAERAGGADGGYGVPSDIVRKALANVGSAPLETACVER"
     gene            complement(2480..4291)
                     /locus_tag="AC150V_01460"
     CDS             complement(2480..4291)
                     /locus_tag="AC150V_01460"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HIR3761621.1"
                     /translation="MADNFLERLRTGPPIVADGGMGALIGGAVANLRCPEEANIRYPE
                     SVIAVHISYIHAGAELIETNTFGANRRKLARHFLEDEFATINSAGVRLAREAREVAGR
                     SVFIAGAIGPLGELEVFDASEHGPLYAEQAEILEGRGVDVLCVETFFDLDELIAAVAA
                     IRNVSALPIVAMLTFDEDAEVSGGISARQAAEDLAGLDVTAIGTNHGAGPYAALTAIS
                     RMESAGLPLVAMPNIGLASISEGRVVYPHSTPEYFAEFAMQATELGARIIGGCCGTTP
                     AQTEAIREALDSGRTPAASFEPAETELRLVRAASAEETELARALREGEWVVSVELDPP
                     KGGRLDGLVEVARTLRRSGAVGFVDVNDNPMARARMNALIASIHLQREAGIETIPHVT
                     PRDTTIMGLEGVLLGAHAEGVRNILAVTGDPPHVGDYPGSRGVYEVDSIGLVQMLARL
                     NEGVDYLGKAIDAPASFYIGVAVNPSADDLELELERFERKIEAGAHFAMTQALFDLSY
                     LDRFLERLGGESPIPLLVGIWPLRSHAMALRLHNEVPGIVVPADVLVLLADAGSEAAD
                     AGLEFARTQVEEIRGKVAGIYVIPPFKRPEGALDLLL"
BASE COUNT          728 a         1631 c         1679 g          764 t
ORIGIN      
        1 tacgcgaagg tcgtcgtgaa ggtcgagact ggcttctgtg tcgccaacaa catcaccttc
       61 ccgtcgacgg tctacgaagg cgccgtgggc gcgctgtcct tcacgaatgc cgcgcacgtg
      121 ccgtcaagcg ggggcaccct gcccgtcaac accacgcact gctaccggtt cagcttcaag
      181 ctggccgacg acgccggcaa cgccctccag ggcaagtctg cgacgttcaa cgtcatcatc
      241 gacgcgacgc agcctgagaa ccccggctgg agcgagtagt cgaataagcg tttcgggggg
      301 gcggctaccg ccgccccccg acgcaatgga gcagcgatgg ctcgtcgggc acaacaggca
      361 ctcgatcgca tggccgtcct ttcgaccggg cgcccggtcg agaagcccaa tggacggccg
      421 cgcgatctgc ggcgcgttgc aagtgccgcg acgtccgtgc tgctcgctgt cggactcctc
      481 ttcgccgtgt tcgtcgtgta cggctcggcg atcgaaaacc gctggtacaa gatcgtcgcg
      541 atcgaaggcg gcagtatgga gcccacgatg cacgcaggag acgcgatcgt cgtctcgcgg
      601 cctcccaaga cgctggagaa aggcatggtg gtgctcctcc agatcaacga caagctcgtc
      661 acgcaccgcg tgctggccgt caaacccgat ggaagtttca cgacgaaggg cgacgcgaac
      721 gactacgcgg acgactggac aggaaccggt gtccgcgtcg ccggcgtcta ccgcgccaag
      781 atcccgctgc tcgggcgagc gctcagcctg ggatcggggg cgtggttggt ggaccgagcg
      841 cagacaccgg taactgcaac cggcggttga cacttgagcc gcctgacagc cgcgtcacct
      901 tccacgacct ccgccgcacc tacgcctcac acttgatccg ggcggacgcc gaccccgtcc
      961 gtgccgcacg ccggttcagc cacgccgacc cgctttcacg ctccgcgtct acgctcggga
     1021 cttcgccgcg gtaagcggcc gggacgaggt cagcgagaag ctgatcgcgg cgttcgccgg
     1081 ggacgtctag aggccaaaac tgacgtttcc tgacatctca gcctctcgac aggagcgcct
     1141 ggcggatcaa ggagatccgc cgtttccagc gggttcctgg acgggagcga cgggactcga
     1201 acccgcgacc tccggcgtga caggccggcg ttctaaccaa ctgaactacg ccccccgtgg
     1261 agcgggcgca ttgtagccgt gtaccgacgc gcgcgcggcc tcggggaagg gacacgcacg
     1321 ggcgccccgt aaccctgggt tggtgacggt cctcgacctc gtcctgctcg gcgtgctcgc
     1381 gctgatggcg ctcggcgggt tccggcgcgg gcttctcgtg ggcgtcctat cgctcgccgg
     1441 gctggttgcc ggtgcgtacg tgggtgcccg tgtggctccg gggttgctgg acgacggctc
     1501 gcgctggatt ccggttgcca cgctggtcgg cgccgtggtc gcggcagcgc tcgggcagtg
     1561 gggagcggtg accgccggcc ggtcgctccg tgctctcgtc acgatcgggc cgctcagcgc
     1621 tctcgaccgc gtcggcggag ccgtcctcgg catcgcgacc ggcctcgtct tctgctgggt
     1681 gctcggcgcg gtgctgctct atctgcccgg tcagacggag ttgcgccggt acgcgcagga
     1741 gtcggcgatc ctctcgacgc tcaaccagga gttcccgccg catcggctga tggacgcgct
     1801 cagtcgcgta gatcccttcg cgtcgctggc gggcccggcc gcgaacgtcg cccctccgga
     1861 gccggcgatc gccggcgatc cggacgtcat cgcagcacgg ctcggcgtcg tgcgcgtaac
     1921 agggttcgcc tgtgggctcg gtgtcgaggg ctccggatgg attgccgggc cggagctcgt
     1981 ggtgacgaac gcacacgttg ttgcgggcgt cgacgttccg cgcgtggatc agggagaccc
     2041 gcggctgtac cagggcgccg tcgttcactt cgacgcacgt aacgacctgg ccctgattcg
     2101 ggtgccgggc ctgcgcggag cccagctcca gctgggtgac cccgagcgtg gattggctgt
     2161 cgcgatgctc ggtttcccgc aaaacggccc gtaccaggca actcctgcgc gcctgggagc
     2221 caccgtgacg agcgtcgggc gggacgcata cggtcgcttc cccgttccac gatccgtgac
     2281 gacgatacga ggcgacatcg tctccggcaa ttccggaggc ccggccgtcg acgccgacgg
     2341 acgcgttcgc acgacgatct tcgcggagcg ggcgggcggc gcggacggcg gctacggcgt
     2401 cccgagcgac atcgtgcgca aggccctcgc gaacgtcggc tcggcaccgc tcgagacggc
     2461 ctgcgtcgag cggtagccct cagaggagca ggtcgagagc gccctccggc cgcttgaacg
     2521 gcgggatcac gtagatgccc gcgaccttgc cgcgaatctc ctcgacctgc gtccgggcga
     2581 actcgaggcc agcgtcggct gcttcagagc cggcatcggc gagcagaacg agaacgtcgg
     2641 ccggaacgac gattccgggc acttcgttat ggagccgcag cgccatcgca tggctgcgga
     2701 gcggccagat gccgaccagg agcggtatcg gcgactcgcc gccgagcctc tccaggaacc
     2761 ggtcgaggta cgagaggtcg aagagcgcct gggtcatggc gaagtgcgcc ccggcctcga
     2821 tcttgcgctc gaaccgctcg agctcgagct cgagatcgtc ggcagacggg ttcaccgcaa
     2881 cgccgatgta gaacgaggca ggagcgtcga tggccttgcc caggtagtcg acgccctcgt
     2941 tgaggcgggc gagcatctga acgagcccga tcgagtcgac ttcgtagaca ccccgcgagc
     3001 cggggtagtc gccgacgtgg ggagggtcgc ccgtgacggc gagaatgttg cggacaccct
     3061 cggcatgggc gccgagcaga acgccttcga ggcccatgat cgtcgtgtcg cggggggtga
     3121 catgcggaat cgtctcgatc cccgcctcgc gctgaaggtg gatcgaggcg atcagcgcgt
     3181 tcatgcgggc ccgcgccatg gggttgtcgt tcacatcgac gaagccgacg gcgccggagc
     3241 gccgcagcgt gcgtgcgacc tccacgaggc cgtccagccg tccgcccttc ggcgggtcga
     3301 gctcgaccga gacgacccat tcgccctccc gcagagcacg ggccagctcg gtctcttccg
     3361 cggatgcggc gcgcaccaac cgcagctcgg tctctgccgg ctcgaacgag gcggccggcg
     3421 tgcggcccga atccagggcc tcccgaatgg cctctgtctg cgcgggcgtc gtgccgcagc
     3481 atccgccgat gattcgggca ccgagctccg tggcctgcat ggcgaactca gcgaagtact
     3541 cgggggtcga gtgcgggtag acgacccggc cctccgagat gctggccaga ccgatgttgg
     3601 gcatcgccac gagcggtaga cccgccgact ccatgcggga gatcgccgtc agggcggcgt
     3661 acggaccggc gccgtgattg gtgccgatcg ccgtgacgtc gagcccggcg aggtcctccg
     3721 cagcctgccg tgcgctgatg ccgcccgaga cctccgcgtc ctcgtcgaag gtgagcatgg
     3781 ccacgatcgg aagcgcggag acgtttcgga tcgccgcgac ggctgcgatc agctcgtcca
     3841 ggtcgaagaa cgtctcgacg cacaggacgt cgacgccgcg gccctcgagg atctcggcct
     3901 gctcggcgta gagcgggccg tgctcgctcg catcgaagac ctcgagctca ccgagcgggc
     3961 cgatcgcccc ggcgatgaag acactccgtc cggcgacctc tcgcgcctcc cgcgccagcc
     4021 ggacacccgc ggagttgatc gtcgcgaact cgtcctcgag aaagtggcgg gcgagcttgc
     4081 ggcggttcgc tccgaacgtg ttcgtctcga tcagctccgc gcccgcgtgg atgtaactga
     4141 tgtgcacggc gatgacgctc tccggatacc ggatgttggc ttcctccggg cagcgcaggt
     4201 tcgcgacggc accgccgatg agagccccca tgccgccgtc ggcgacgatc ggcgggccgg
     4261 ttcgcaggcg ctcgaggaag ttgtcggcca cgcccgcact ctagaagccc ccgcaccgcg
     4321 gggcccgccg tgtcaactac caatccgagt gattgccaat ccgccgttcg cgcagatact
     4381 gagtgcatct cgccccgacc gcgactgcgg cgtccgcgac gggacgagcg atccggttcc
     4441 ccgtggggaa cctgcagaaa ggagcggggc ctggcggcgg ccccgcttct tgctttcgtc
     4501 cctcgtacga ctcttcagca cccgctggcc gctgccgatg agcagtgtga ctcctggtcg
     4561 gggactgacg gcaatcgctc ccacagaccg agtccggcgg gcggcttcgg ccgcccgctg
     4621 tggtttcgac ggcccttgga cttcgcgatg gcccgcgatc gttagggcgc cgtctcgtag
     4681 acgcgggttt cccagaagga gatgccgtag ggggtggcgc gggtgaggcc gaggatgcgg
     4741 acgtagcggg cgttgcgggc ggggaaggtg gtgcggatga tgccggcgct ggttggtgtt
     4801 tc
//
LOCUS       DBNGNK010000056         4923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1943197,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000056 DBNGNK010000000
VERSION     DBNGNK010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1943197"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC150V_01465"
     CDS             <1..311
                     /locus_tag="AC150V_01465"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019045412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HIR3761622.1"
                     /translation="RIEQPLIDELERACDEFNEGLPELRSFVLPGGCEAAARLHVART
                     ACRRAERAALAAAGEHELNPLTIVYLNRLSDLLFILSRAANALAGADEPLWKPGATAS
                     "
     gene            284..586
                     /locus_tag="AC150V_01470"
                     /pseudo
     CDS             284..586
                     /locus_tag="AC150V_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181491.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
     gene            559..741
                     /locus_tag="AC150V_01475"
     CDS             559..741
                     /locus_tag="AC150V_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761623.1"
                     /translation="MGGVHASVCLLAAAFWDPVLSRAVGSVWDALLVAVVFAALRFVP
                     PWMVVPAAAGLGTLSF"
     gene            complement(738..2222)
                     /locus_tag="AC150V_01480"
     CDS             complement(738..2222)
                     /locus_tag="AC150V_01480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3761624.1"
                     /translation="MGRPRPGIASRLGWALAAAAFVAAGVAVGALLGGGESIWPVVAG
                     GAALCGLAAVAVARLVASLRDAQLAALSDPLTRLPNRVLLLDRVEQAVRRSRRSGEPF
                     ALVVVDLDGFKNVNDNHGHASGDVVLCELARRFEDTVRTSDTVARVGGDEFVILSLGS
                     RDEHEVAALVGRLRQALREPIAFGNDDFARVDASIGWAIHPTDGATAAALIDRADEQM
                     FATKREEGEARRTRQRRPSGAVLRSLERALHQGRVVVHYQPVVELSSGKPRRAEALVR
                     GCTTARGVLTPVELADSVARTPLASALALNTVAEALRRAEEWRSAGHVIGVAVNVPAR
                     ALEDDTLGDGIARLLNQTRTPVDLLTLELGSSDGADAGMDPEAVAALARLGVRISLAD
                     VIRSSSVAALRSVPLDELKIDASFVRGLVGTPADAAIVRALVDAGRELGFEIVAEGVE
                     SPETLSELVRIGCHLAQGPYLGHPMPGDELLSWLDERRRSREAV"
     gene            2411..4492
                     /locus_tag="AC150V_01485"
     CDS             2411..4492
                     /locus_tag="AC150V_01485"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR3761625.1"
                     /translation="MASRASSASSAGLEPSRLAAACASATAALAGADDPGAAVDSALH
                     ALHADVDEALVSVFVLEHERLWITAQRGYTMIPDGLAIEDGIMGRAVRTRRAQLVRDV
                     ATDPDYVAAAHGVVSQLSVPLQRNGQVFGLFTIESRAELPPESTRLVRPLTRRLVSLA
                     EVLRAAPQFDLSALARLFVYVSSLRDPEAIADIAARSLARVLSVETSQVALFSDDGSI
                     EQAAGWRAAASSLNVFSTDELALLRSCAETTAVVDVIAAADHDLDGPARSIVWLPLRA
                     SAAELGVLVGSSADVGELDRSRAEVASLLAAHAAASLDAALSLGRERQNAATDSLTGL
                     LNRRGFEERLELQLRESQDQRRPLSVLVLDCDDFKAVNDRAGHEFGDALLREAGHALS
                     SLLPPTAWCARLGGDEFVVLFADSDVDATQALGDGLRVQLAARLADAGFPLHVSAGFA
                     TYPIDGAGAPALLRGADQALYVAKELGKDRVLSFRDALGDPTEKAGSDVRRRERRRAS
                     FAPKGTSLNDMADAADAVWDGTCSQDVLERLCKSLTFVVGATGCLASRVDGGYLYDVA
                     RHSIRNVDFGEDSAYLISDFPLTEQVLGSGQSRSVSFLEPDIDRAEAFVLRDLDMNCV
                     LLLPLIVRSRPWGLVELYDVRLRHFGPSEQGVAEFLARQAVRRIETLTGDDSDRAHGL
                     PVVRVPRADGH"
     gene            complement(4489..>4923)
                     /locus_tag="AC150V_01490"
     CDS             complement(4489..>4923)
                     /locus_tag="AC150V_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761626.1"
                     /translation="KELESRAQRLHEAAAESERLRRQLAAREASLVERELRLGDAEES
                     ARAVASLEAELERTRRELEIGRAQASAGTTTPEPVHDDHLVFVPAPNSYRLLERSGVP
                     PDPGDSIEVEGARFRVTRVGPSPLPGDSRRCAYVERDADVAG"
BASE COUNT          677 a         1765 c         1682 g          799 t
ORIGIN      
        1 tgcggatcga gcagccgctg atcgatgagc tggagcgcgc gtgcgacgag ttcaacgagg
       61 gcctgcccga gctgcggagc ttcgttctgc cgggcgggtg cgaggcggcg gctcgcctgc
      121 acgtggcgcg caccgcgtgc cgtcgggccg agcgggccgc gctggccgca gcgggcgagc
      181 atgagctgaa tccgctgacg atcgtctacc tcaaccgcct ctccgacctc ctgttcatcc
      241 tctctcgggc cgcgaacgcg ctcgccggcg ccgacgagcc cctgtggaag ccgggagcca
      301 cggccagcta gagccgagtc cccggcacgc tctcgacgtc ttcctcgccg cattgcggct
      361 cggcgtgacc tcgttcggcg gtccagtggc tcacatcggc tatttccgca ccgagtacgt
      421 ccggcgccgc cgctggctcg acgacgcgac cttttccgag ctcgtcgctc tcacgagcgt
      481 tctgcccggg cccgccagca gccagctcgg catcgccatc ggcatgcttc gcgcgggcaa
      541 gctcggcggc ctgcttgcgt gggtggggtc cacgcttccg tctgcctgct cgcggcggcg
      601 ttctgggatc ccgtgctgtc gcgtgccgtc ggcagcgtct gggacgcgct cctcgtcgcc
      661 gtcgtcttcg cggcgctccg cttcgtgccg ccgtggatgg tcgtccccgc cgcggctggc
      721 ctaggaaccc tctcgttcta gaccgcttct cggctgcgtc ggcgctcgtc gagccacgac
      781 agcagctcgt cgccgggcat cggatggccg aggtacggtc cctgcgccag gtggcagccg
      841 atccggacga gctcgctcag cgtctccggg ctctcgactc cttcggcgac gatctcgaag
      901 cccagctcac gcccggcgtc gacgagcgcc cgcacgatcg ccgcatcggc gggcgtcccg
      961 acgagaccgc gcacgaagct ggcgtcgatc ttcagctcgt cgagcgggac ggagcgcagc
     1021 gcggcgacgg acgacgagcg tatgacgtcg gcgagggaga tacgaacgcc cagccgtgcg
     1081 agcgccgcga ccgcttctgg atccatccct gcgtcggcgc cgtcggacga cccgagctcg
     1141 agcgtgagga gatcgacggg cgtgcgagtc tggttgagca gacgcgcgat gccgtcgccc
     1201 agcgtgtcgt cttcgagcgc gcgggcgggg acgttgaccg cgacgccgat cacgtgacct
     1261 gcgcttcgcc actcctcggc acgccggaga gcctcggcga cggtgttgag cgccaatgcg
     1321 gaagcgagcg gggtgcgtgc gaccgagtcg gcgagctcga cgggggtgag cacgccgcgc
     1381 gccgtcgtgc agcctcgcac cagcgcttcc gcccggcgcg gctttccgga gctgagctcg
     1441 acgacgggct ggtaatgaac gacgacccgt ccctggtgga gcgcgcgctc gagcgatcgc
     1501 agcacggccc cactgggtcg ccgctggcgc gtacgcctgg cttcgccctc ctcgcgcttt
     1561 gtcgcgaaca tctgctcgtc ggctcggtcg atcagcgcgg cggcggttgc tccgtcggtt
     1621 gggtgaatcg cccagccgat gctcgcgtcg acgcgcgcga agtcgtcgtt gccgaacgcg
     1681 attggctcgc gcagcgcctg gcgcagacgc ccgacgaggg cagcgacctc gtgctcgtcg
     1741 cgagacccca gcgagaggat cacgaactcg tcgccaccga cgcgcgcgac ggtgtcgctg
     1801 gtacgcacgg tgtcctcgaa gcgccgcgcc agctcgcaga gaacgacgtc gccgcttgcg
     1861 tgcccgtggt tgtcgttgac gttcttgaag ccgtcgaggt cgacgacgac gagcgcgaac
     1921 ggctcaccac tccgtctcga gcggcgaacg gcctgctcga cgcggtcgag cagaagcacg
     1981 cggttgggaa gtcgtgtcag gggatcgctg agcgccgcaa gctgggcgtc gcgaagggag
     2041 gccacgaggc gggcgaccgc gacggcggcc aggccgcaca gggccgcgcc acccgcgacg
     2101 acgggccaga tggactcacc gccgccgagc aatgcgccga cggccacccc tgccgcgacg
     2161 aaagcggctg ctgccagcgc ccagccgagc ctgctggcga ttcctggtcg cggtctcccc
     2221 attttcacgc cgtcttgtgc tgattgtccg caggtttgcc gctcaatcag ttatctgacc
     2281 gtacgagcga aaggggggaa gtgcttgcct cgtccgaggg aaccacagtc cctcgctcgg
     2341 gggagagtgg gtcgggccca cgtcggccgc ccctcaagcg agggatgtgc gcgtccgatg
     2401 ccactcggtg atggcgtcgc gcgcatcctc ggcttcgagc gccggactcg agcccagccg
     2461 gctcgccgct gcctgcgcaa gcgccacggc tgcgctggca ggggcagatg accccggcgc
     2521 cgccgtcgac tcggcgcttc acgcccttca tgccgatgtc gacgaagcac tcgtctccgt
     2581 gttcgtgctg gagcacgaac ggctctggat caccgcgcaa cgtggctaca cgatgattcc
     2641 cgacggactc gcgatcgagg atggaatcat gggccgcgcc gttcgcactc ggcgggcaca
     2701 gctggtgcga gacgtcgcga ccgatccaga ctacgttgcg gcggctcacg gcgtggtatc
     2761 ccagctcagc gtgccgctcc agcgaaacgg ccaggtcttc ggcctcttca ccatcgaaag
     2821 cagggccgag ctgccgcccg agtcgacacg cctcgtccga ccgctcacgc gccgcctcgt
     2881 ttccctcgca gaggtgctcc gagcagcgcc tcagttcgac ctctccgcgc tcgcccgcct
     2941 cttcgtgtac gtgagctctc tccgagaccc ggaagcgatc gccgacatcg ccgcacgttc
     3001 gctcgcgcgc gtgctttccg tcgagacgag tcaggtcgcg ctcttcagcg acgacggatc
     3061 gatcgagcaa gcggccgggt ggcgcgccgc tgcgagcagc ctcaacgtgt tcagcaccga
     3121 cgagctggcg ctcctgcggt cgtgtgcaga gacgaccgcc gtcgtcgacg tcatcgcggc
     3181 agccgaccac gacctcgacg gcccggctcg ctccatcgtc tggctcccgc tacgtgccag
     3241 cgcggccgag cttggcgtgc tcgtcggctc gagcgccgac gtcggtgagc tcgaccgcag
     3301 tcgggctgag gtcgcttcgc tcctcgcggc tcacgccgcc gcgtcgctcg acgccgctct
     3361 gtcgctcgga cgtgagcgcc agaacgcagc gaccgactcc ctcaccggcc tgctcaaccg
     3421 ccgaggcttc gaggagcggc tcgagctcca gcttcgggag tcgcaagatc agcgtcgccc
     3481 gctcagcgtg ctcgttctcg actgcgacga cttcaaggcc gtcaacgatc gcgccggaca
     3541 cgagttcggc gatgccctgc tgcgcgaggc cggtcacgcg ctcagctcgc ttctgccccc
     3601 gaccgcgtgg tgcgcgcgcc tgggtggaga cgagttcgtc gtgctgttcg ccgactctga
     3661 tgtcgacgcg acacaggccc tcggagacgg gctccgcgtc cagctcgccg cccggcttgc
     3721 agacgcaggc tttccgctgc acgtgagcgc cggcttcgcg acatatccca tcgacggtgc
     3781 cggcgccccg gccctactgc gaggagcgga tcaggcgctc tacgtggcga aagagctcgg
     3841 caaggatcga gtgctcagct tccgtgacgc cttgggcgac cctaccgaga aggccggctc
     3901 ggacgttcgc aggcgggagc gacggcgcgc gtcgttcgct cccaaaggaa cctctctgaa
     3961 cgacatggcc gatgcagcgg atgccgtgtg ggacggaacg tgcagccaag acgtgctcga
     4021 acggctctgc aagtcgctga ctttcgtggt gggcgcgacg ggctgcctcg cgtcgagggt
     4081 tgacggcggc tacctctacg acgtcgcgcg ccattcgatt cgcaacgtcg acttcggcga
     4141 ggattctgcc tacctgatca gcgacttccc gctcaccgag caggttctcg gctcgggcca
     4201 gagccgctct gtctcgttcc tcgagccgga catcgaccgg gccgaggcct tcgttctccg
     4261 cgatctcgac atgaactgcg tgctgttgct gccgctcatc gtgcgctccc gtccctgggg
     4321 cctggtagag ctctacgacg tgcggctacg gcacttcggc ccgtccgaac agggtgtcgc
     4381 cgagttcctc gcgcgccagg ctgtccgccg catcgagacc ctgacaggcg acgattccga
     4441 ccgagcgcac gggctgcccg tcgtccgcgt gccgcgggca gacggccact agccggcgac
     4501 gtccgcgtcg cgctcgacgt acgcgcagcg acgggagtcg ccggggagag gtgacgggcc
     4561 gacgcgggtc acgcggaagc gtgctccttc gacctcgatc gaatcgcccg gatcgggtgg
     4621 cacgccggag cgctccaaca gtcggtacga gttcggcgcc ggaacgaaga cgaggtggtc
     4681 gtcgtgaaca ggctccggcg ttgtggtgcc agccgaagct tgcgcgcggc cgatttcgag
     4741 ctcgcgtctc gtccgctcca gctcggcctc gagcgaggcg acggcgcgtg cgctctcctc
     4801 cgcgtcgccc agccgaagct ctcgctcgac gagcgacgcc tcccgagcgg cgagctgccg
     4861 tcgtaggcgc tcactctccg ccgctgcttc gtgcagccgc tgtgcccggc tctccagttc
     4921 ctt
//
LOCUS       DBNGNK010000057         6201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_9494,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000057 DBNGNK010000000
VERSION     DBNGNK010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6201
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_9494"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..1883
                     /locus_tag="AC150V_01495"
     CDS             243..1883
                     /locus_tag="AC150V_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR3761627.1"
                     /translation="MLRRLRIENLALIREAELELSDGLNVVTGETGAGKTIFAQSIGL
                     LLGGRGDAAIVGPAAAEAYVEAELDLPAGFFDDEDLAALADVKPEGEEGLVLARRVFG
                     DGRTRAYAWGRAVTRDDLVATGERLIAMSGQFEQRRLARASFQLDVLDAYCGAEQLRR
                     RQESAAGWRELIAARRRADELARDAAASTAQQAELEALVADTVSFVAGEEGELRAERE
                     RLLHVTELGASASAAVQAIAPEDGDGAASLAAAAERQLAPYEAIAPELASIARELGEL
                     ALRLREAGSDLHRFIASVEADPDRLQAVEQRLELFADTKRRYRCATYEELCARREEAE
                     AELRRLADDGDPVAAAAAEQAAAEMRVAGLADALAESRRAAIAPFSVDVAAELRSVGM
                     GDGEFRVEVRAREPGPTGGDEAVFLVRPNQGLPYAPVAETASGGELSRIALALAAVAG
                     GETLVFDEIDAGIGGETAHRVAETLRRLAGRAQVITITHLPQIASGADSHFRVEKTPG
                     DPTLTRIERLDDDARRVELERMAGGREFIATLAAGDPS"
     gene            1953..3593
                     /locus_tag="AC150V_01500"
     CDS             1953..3593
                     /locus_tag="AC150V_01500"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR3761628.1"
                     /translation="MSSTKYVFVTGGVVSALGKGIVAASLGRLLKARGLSVQAQKFDP
                     YLNVDPGTMSPFQHGEVFVTEDGAETDLDIGHYERFIDENLSRNASHTAGAIWDTVLR
                     KERRGEFLGATVQVIPHITNEIKARIRRGGAATPTDVVISEIGGTVGDIESLPFLEAI
                     RQFRREVGPENVVYIHVTLVPFIEAAGELKTKPTQHSVNELRRIGIHPDIVVCRSREE
                     LSTEIREKIALFADVDPRAVVASPDVPDVYVVPQVLNSEGLDDLVCSELALETGPPEL
                     GEWEELTARAAELVEDVEIALVGKYVKLHDAYLSVHEALKHAGVHNGCRVHVRWVDAE
                     NMDYEEIAELLEGVGGVLVPGGFGSRGWEGKILACRAARERSIPYLGICLGMHVAVSE
                     FARHVCGLEGANSTEMDPETPYPVIDLLPEQKEIEDLGGTMRLGAQAVELVDGTRAQG
                     AYGEEVIHERHRHRYEVNNRLRQTLSDAGLVVSGTFQGGRLVEIVELAEHPWFVASQF
                     HPEFKSRPTRPAPLFREFVGAALERTRSRSGVRLETAS"
     gene            3656..4777
                     /locus_tag="AC150V_01505"
     CDS             3656..4777
                     /locus_tag="AC150V_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3761629.1"
                     /translation="MATPPLLDHFLELCSIPSPPGEERPIADRVLEYLAGLGLEADED
                     DAGPRVGANAGNLLCRLPGGNGSGTPLFFCAHLDTVPPLGPIEPIVEDGIVRNGGGTI
                     LGADNKAAVVVMLEAARRIVSEARPHAGVELLFTPKEEVGLLGAGAFDVSRLEARLGY
                     VYDQAASIGEIILRAPSAKAISAEFTGRAAHAGMYPEDGRSAIAAAARAVADLRLGRV
                     DDETSANVGLIAGGSARNIIPESCSVEAEARSHDDAKLGELVQEMVDTIAYAASVSDC
                     TVETTIVEQYRGYRFREDDLVVRIAEAGLRQSGFEPRTAVSGGAADANVFNAQGLACV
                     NLANGMAEIHTPDEHIAVVDLERMVDVTLALVDTARDAS"
     gene            4767..5738
                     /locus_tag="AC150V_01510"
     CDS             4767..5738
                     /locus_tag="AC150V_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HIR3761630.1"
                     /translation="MPLSLRWGSVTAVTERLDELVRLEVDGVPCLAFPRLTGPVEQGD
                     TVLVNTSSAAGMESTEVLYANLTRGIGLQPAQDSVAVTLPATPAQAAVPLGENLLDGA
                     AEVDGLPVLCCTRHSQVAAACAGLGGGRIAYVQVDGGALAVSYSGTVRALRNRGAIEA
                     AVAVSPCIDGDVQCLTVAGALVWAKVGGFRAAVCAPGPGGAAIGPVPSPGVVAAAAAA
                     NAASALGGRPIVAPCVSLAGSADEREELAEETRAALDLCLDDVVVAWPVGLPPPTDLN
                     TVAVDVEGWDVACVGLPLIHDGRGPEIDPWFFKAAYAAGRVADWNLV"
     gene            5735..>6201
                     /locus_tag="AC150V_01515"
     CDS             5735..>6201
                     /locus_tag="AC150V_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3761631.1"
                     /translation="MIEHRRLGPVIGLGTYGTFDRDAERASEVVAAALSAGVTLFDSS
                     PMYGGAERSLGDALRTRRDGTSIATKIWTPDLETGRTQYAAQQEFFGRVEIEQVHNLV
                     AWQEHLPWLEAEREAGRIDRVGVTHYSPSSFDELERALRGGRFQTVQVPLNPL"
BASE COUNT          976 a         2078 c         2207 g          940 t
ORIGIN      
        1 ccggacacgc cgacgccgga ttcacgttga aggcgtacgc cagagatgcg cgtccgcagg
       61 agaccgtggt tgcggatgtg ctggagcggg ctgcgacggc cggagtgggc cggtgaggtt
      121 agtcccgttt ttagtcccgt ttggttgcgt cctgctgacg actcgtgatg actggcacaa
      181 ttgtgcaaac gcattccaag cggttttgac ggttcctgac cacccgagac gacccgctgg
      241 ccgtgctccg taggctgcgc atcgagaatc tcgccctgat ccgcgaggcc gagctcgagc
      301 tctcggacgg actcaatgtc gtgaccgggg agaccggagc gggaaagacg atcttcgccc
      361 agtcgatcgg gctgctcctc ggcggccgcg gcgacgccgc catcgtcggg cccgccgcgg
      421 ccgaggcgta tgtcgaagcc gagctcgatc tcccagcggg cttcttcgac gacgaggatc
      481 tcgccgcgct cgccgacgtc aagcccgagg gcgaggaagg gctggtgctg gcgcggcgcg
      541 tcttcggcga cgggcggacg cgtgcctacg cctggggccg cgccgtcaca cgggacgatc
      601 tggtcgccac cggcgaacgg ttgatagcca tgtcgggcca gttcgagcag cgccggctgg
      661 cccgtgcctc attccagctc gacgtcctcg atgcctactg tggagcggag cagctccgcc
      721 gccgacagga gtcagccgcg ggatggcgag agttgatcgc ggcccgccgg cgcgccgacg
      781 agctggcccg cgacgcggcc gcgtcgacgg cgcagcaggc ggagctcgag gcgctcgtcg
      841 ccgacacagt cagcttcgtt gcgggcgagg aaggcgagct gagagccgag cgggagcggc
      901 tgctgcacgt caccgagctc ggcgcgtcgg cctcagcggc cgtccaggcg atcgcgccgg
      961 aggacggcga cggggcagcg tcgctggccg cagcagcgga gcgccagctc gcgccctacg
     1021 aggcaatcgc cccggagctg gcaagcatcg cgcgagagct cggcgagctc gcactgcggc
     1081 tgcgggaggc gggaagcgac ctgcaccggt tcatcgcgtc cgtggaagcc gacccagatc
     1141 gtctgcaagc ggtcgagcag cggctcgaac tgttcgccga tacgaagcgc cgctaccggt
     1201 gcgccacgta cgaggagctc tgtgcccgtc gagaggaggc ggaggccgag ctccggcgtc
     1261 tggctgacga tggcgatccg gtcgccgctg cggccgcaga gcaggccgca gccgagatgc
     1321 gtgtcgccgg cctcgccgat gcgctcgcgg aatcacgccg tgccgccatc gcgccgttct
     1381 cggtcgatgt ggcggccgag ctgcgaagtg tcggaatggg agacggcgag tttcgcgtcg
     1441 aggtgcgcgc gcgcgagccg ggaccgaccg gtggagatga agcggtgttc ctggtgcgtc
     1501 cgaatcaggg gcttccgtat gcgcctgtcg ccgagaccgc atcgggcggc gagctctctc
     1561 ggattgccct cgccctggcc gccgtagcgg gcggcgagac gctcgtgttc gacgagatcg
     1621 acgcaggtat cgggggcgag acggctcatc gtgtcgccga gacactgcgg cggcttgcag
     1681 ggcgcgcgca ggtcatcaca atcacccatc tcccgcagat cgcgagcggc gccgactcgc
     1741 atttccgggt cgagaagact ccgggcgacc cgacgctcac gcggatcgag cgactcgacg
     1801 acgatgcgcg gcgcgtcgag ctggagcgga tggccggcgg gcgcgagttc atcgccacgc
     1861 tcgcggcggg cgatccttcc tgaccccgtg ctagctttgg atcccgtgcg gtcgctgacg
     1921 ggcaggcagc agaacggcgc ggtggcgcgc ccgtgagttc gacgaagtat gtcttcgtga
     1981 caggtggagt cgtctcggcc ctcggcaaag gaatcgtcgc ggcatcactc ggtcgtctgc
     2041 tcaaggcccg cggcctcagc gtgcaggccc agaaattcga cccgtatctg aacgtcgacc
     2101 cgggcacgat gagccccttc cagcacggcg aggtgttcgt caccgaggac ggggcggaga
     2161 ccgatctcga catcggtcac tacgagcgct tcatcgacga gaatctgtcc cgcaacgcga
     2221 gccacaccgc aggcgcgatc tgggacaccg tgctccgcaa ggagcggcgc ggcgagttcc
     2281 tcggcgcgac ggtacaggtg atcccgcaca tcacgaacga gatcaaggcg aggatcagac
     2341 gcgggggtgc cgcaaccccg acggacgtcg tcatatcgga gatcggcggt accgtgggcg
     2401 acatcgagtc gcttccgttt ctcgaggcga ttcgccagtt ccgacgcgag gtcgggcccg
     2461 agaacgtcgt ctacatccac gtcacgctgg ttcccttcat cgaggccgcg ggagagctca
     2521 agacgaagcc gacccagcat tcggtcaacg agctccgtcg catcggcatc catcccgata
     2581 tcgtcgtctg ccgctcacgg gaagagctct cgacggagat cagagagaag atcgcgctgt
     2641 tcgccgacgt cgacccacgc gccgtcgtcg cctcgccgga tgtgccggat gtctacgtcg
     2701 tgccgcaggt gctcaactcg gaagggctgg acgacctggt ctgctcggag ctcgcgctcg
     2761 agacgggccc gcccgaactc ggcgagtggg aagagctgac ggcccgtgcc gcggagctcg
     2821 tcgaggacgt cgagatcgcc ctggtgggaa agtacgtgaa gctccacgac gcatacctgt
     2881 cggtacacga ggccctgaag cacgcgggcg tgcacaacgg ctgccgcgtc catgtccgat
     2941 gggtcgacgc agagaacatg gactacgagg agatcgcaga gctgctcgag ggcgtcggcg
     3001 gcgtgctcgt cccgggtggt ttcgggtcgc gtggctggga ggggaagatc ctcgcctgcc
     3061 gcgctgcgcg tgagcgctcg attccctacc tcggcatctg cctcggcatg cacgtcgcgg
     3121 tctcggaatt cgcgcggcac gtctgtggtc tcgagggcgc gaactcgacc gagatggatc
     3181 cggagacgcc gtacccggtg atcgacctgc ttcccgagca gaaggaaatc gaggatctcg
     3241 gcgggacgat gcgcctcggg gcgcaggcgg tcgagctggt cgacggtacg cgagcccagg
     3301 gtgcctacgg cgaggaggtc atccacgagc gccaccgcca tcgctacgag gtgaacaacc
     3361 gcctccgaca gacgctctcg gacgcgggac tcgtggtctc ggggacgttc caaggcggca
     3421 ggctcgtcga gatcgtcgag ctcgcagagc acccctggtt cgtcgcgagc cagttccatc
     3481 cggagttcaa gtcgaggccg acgaggccgg cgccgctgtt ccgcgagttc gtcggcgctg
     3541 cgctggagcg gacacgatcc cgcagcggcg tacggctgga aaccgcgtcg tagagagccg
     3601 agcggccgcc cgccgctcca ccgaagcctt cagatccacc atctaggatg gcggcgtggc
     3661 gacgccgccg ctcctcgatc actttctcga gctctgttcg atcccgagcc cgcccggcga
     3721 ggagcgaccg atcgccgatc gcgttctcga gtacctggcg gggctcggcc tggaagccga
     3781 cgaagacgac gccgggccgc gcgtcggcgc caacgccggc aacctcctct gccgcctgcc
     3841 cggcgggaac ggatctggaa cgccgctctt cttctgcgcg cacctcgaca cggtgcctcc
     3901 gctcggcccg atcgagccga tcgtcgagga cggcatcgtg cgcaacggag gggggacgat
     3961 cctaggcgcc gacaacaagg ccgctgtggt cgtcatgctc gaagccgcgc ggcggatcgt
     4021 ctcggaggcc agacctcacg cgggcgtcga gctgctcttc accccgaaag aggaggtcgg
     4081 cctgctcggc gcgggagcat tcgacgtctc gcggctcgaa gcccggttgg gttacgtcta
     4141 cgaccaggcc gcatccatcg gcgagatcat cctccgcgct ccgtcggcaa aggcgatctc
     4201 ggccgagttc accggccgag cggcgcacgc ggggatgtac ccggaggacg ggcgctcggc
     4261 catcgccgct gccgcgcgcg cagtggcaga cctacggctc ggccgggtcg acgacgagac
     4321 gagcgccaac gtcggcctga tcgcgggggg aagcgcccga aacatcatcc ccgagtcgtg
     4381 ctccgtcgag gccgaggcgc gctcgcacga cgacgcgaag ctcggtgagc tcgtgcagga
     4441 gatggtcgac acgatcgcgt atgcggcatc agtctccgac tgcaccgtcg agacgacgat
     4501 cgtcgagcag taccgcggct accgcttccg cgaggacgac ctggtggttc ggattgccga
     4561 ggcggggctg cggcagagcg gcttcgaacc tcgtacggcc gtgtccggcg gcgccgccga
     4621 cgcaaacgtc ttcaacgccc agggcctggc gtgcgtcaac ctggcgaacg gcatggcgga
     4681 gatccacacg cccgacgagc acatcgccgt cgttgacctc gagcgcatgg tcgacgtcac
     4741 cctggcgctc gtcgacactg cccgcgatgc ctcttagcct ccgctggggc tccgtcacgg
     4801 cagtcaccga gcgtctcgac gagctcgtcc ggctggaggt cgacggtgtt ccatgtctcg
     4861 cgttcccacg cctgacgggg cccgtcgagc agggtgacac cgtgctcgtc aacacgtcgt
     4921 ccgcggccgg aatggagagc acagaagtgc tctacgcgaa cctcacacgc ggtatcggcc
     4981 ttcagccagc ccaggacagc gtcgcggtca cgctcccggc aacgcccgcg caggcagccg
     5041 tgccgctcgg cgagaacctg ctcgacggag ctgccgaggt cgacgggctg ccggtgctct
     5101 gctgtacgcg acacagccag gtcgccgcgg cctgcgccgg tctcggcggc ggccggatcg
     5161 cctacgtaca ggtcgatggg ggtgccctcg ccgtctccta ctcgggtacc gtgcgcgcgc
     5221 tgcgaaatcg aggagcgatc gaggcggccg ttgcggtctc tccgtgcatc gacggcgacg
     5281 tccaatgcct gaccgtggcc ggcgcactcg tgtgggcgaa ggtcggcggc ttcagggccg
     5341 ccgtctgcgc gcccggaccg ggcggcgcag caatcggtcc ggttccctca cccggggtcg
     5401 tcgcagcggc ggctgctgcg aacgccgctt cggcgttggg gggtcgtccg attgtcgcgc
     5461 cgtgcgtgtc gctcgccggc tcggcagacg agcgagaaga gctggcggag gagactcgag
     5521 ctgcgctgga cctgtgcctc gacgacgtcg tcgtcgcctg gccggtcggg ctgccgccgc
     5581 cgacggacct gaacacggtc gcggtggacg tcgagggatg ggacgtcgcc tgcgtcggcc
     5641 ttcccctgat ccacgacgga cgcggccccg agatcgaccc gtggttcttc aaagcagcct
     5701 acgcggccgg gcgcgtcgcc gactggaacc tcgtgtgatc gagcaccgtc ggctcggacc
     5761 ggtcatcgga ctcgggacgt acggaacctt cgatcgagat gccgaacgcg cgtccgaggt
     5821 cgtggccgcc gcactgtcgg ccggcgtcac gctcttcgac tcctcgccga tgtacggcgg
     5881 tgcggagcgc tcgctcggag atgccctgcg gacgcgtcgc gacgggacga gcatcgcgac
     5941 gaagatctgg acacccgatc tcgagaccgg ccggacgcag tacgcggctc agcaggaatt
     6001 cttcgggcgg gtcgagatcg agcaggtgca caacctcgtc gcgtggcaag agcaccttcc
     6061 ctggctcgag gcggaaagag aggcggggcg aatcgaccgc gtgggcgtca cgcactattc
     6121 gccctcgagc ttcgacgagc tcgagcgagc gctccgcggt gggcgcttcc agacggtgca
     6181 agtcccactg aaccctctcg a
//
LOCUS       DBNGNK010000058         3053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_511228,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000058 DBNGNK010000000
VERSION     DBNGNK010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_511228"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..>3053)
                     /locus_tag="AC150V_01520"
     CDS             complement(230..>3053)
                     /locus_tag="AC150V_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3761632.1"
                     /translation="SAVLLELISPDQTVTFPYLSTTTSSGGKVIVISGYGTWMGAASV
                     FRNVGAAPLGFYDNLLLPYMVEAIQWLIAPDEPVVGLQLSHAGMTAIARVDDDESERA
                     DLQNPTLDFLVELAETTGVDAALAIVTSLANLGRTWNLFGGPKGEQLEKLGGLMGSHS
                     HVHDYNMSVNLNDAQFNVEVGASLDIVEAELAPIRAMTPPRVFINPGSTIRNEDYSLV
                     FAESDLYFTHGYEQALNYASGVMGFALDPGVAPKPVVVNAPVPDFQWLYTTDPIWVWS
                     PTEVASLQTQILGWFQNSLGRGALYNEMWHDYGMGGDPPMQFPGETAQPLYDAHLAFF
                     ADNEVYAPSVAELIHKLHAASNTYFSSSIDVPSGDLIVTLDLSGLSAEQRAALSGMGL
                     RTSSGTSSIESVTIDGAAHFAFTGDTVVLPPASAASMTARLQLGSGAAGPEPRLEFIS
                     KPFSGFTSGLDEATVALARPDETTKLCVRAAPEFAVLGAASQTRQAPDRLCAQTAHSS
                     NQRKPRVVRLDNTHGLHLLDADRTVELARIEDGNASLLLGGGSAAGNIEFEAQVAPLE
                     VRLDGSPVATTPTADGFAVTLPAGAGPLLDLVYDPADTPETVKPKQLLVYHGRPSAIN
                     GATSLAAAIAEFAQYETVILGDGLQDPAHPDHADTVQIVTALRDAGSTVFGTVRVGVS
                     SGNLPLDEIRQRLDDWNDTNVRGVLLDQFGYEFLTPRDRQNAIVDHAHSQSLVVAVSA
                     EDPADAFDDAIDPNFNPNGEPTRLRSFDFYLWEGHQFRQGAFEAGAAWQSKADALAQL
                     QSSVGFKILSVTTTDVDDPGAFDPDAFFYAWHSAMLYGHEATGWGEFAYSATGVSLDQ
                     APLRTRPEVYPGNYFIGPVLASPPLYRRDTARGEVFVNTSTHAFGFTLHPKVCSLGVH
                     EPAPLSSFLLLALALVCFRRWCASRT"
BASE COUNT          510 a          965 c         1096 g          482 t
ORIGIN      
        1 taccgcgcga tcacccaggt ggtcgcgcgg ttgcctttaa tcgtggcgag gatcgatcca
       61 gccgctcgcc ggggtgcaga agcgcgctcg ccgcgcgcag cggcgcgcgg gccggatccg
      121 agatcgcgtc gcgcagagtc ggatgcgagt ccggccgggt ctggattgaa tagcgcttgc
      181 cgagatccgg gatgcggatc gagacatcgc cgcagcaacc acgctcaaat cacgtccgcg
      241 aagcgcacca tctccggaag cagacgagcg ccagcgcgag cagcaggaag gacgacagcg
      301 gcgcgggctc gtgaaccccg aggctgcaga ccttggggtg cagcgtgaag ccgaacgcgt
      361 gggtcgacgt gttcacgaag acctcgcccc gtgccgtgtc ccgccggtag agaggcggtg
      421 aggcgagcac cgggccgatg aaatagttgc cgggatacac ctccggacgc gttcgtagcg
      481 gggcctgatc gagcgagacg cccgtggccg agtatgcgaa ctcgccccat cccgtggcct
      541 cgtgaccgta gagcatcgcc gagtgccagg cgtagaagaa cgcatcggga tcgaaggccc
      601 ccggatcatc cacgtcggtc gtcgtcaccg acaggatctt gaagcccacg gaggactgga
      661 gctgcgccag ggcgtcggcc ttggattgcc aagccgcgcc agcctcgaag gcgccctggc
      721 ggaactggtg gccctcccac aggtagaagt cgaagctccg gagccgggtc ggctcgccat
      781 tgggattgaa gttggggtcg attgcatcgt caaatgcgtc agcgggatcc tcggcgctga
      841 cggccacgac tagtgactgg gagtgggcat ggtcgacgat ggcgttctgc cggtcgcgcg
      901 gggtcaggaa ctcgtagccg aactggtcga gcaggactcc gcggacgttc gtgtcgttcc
      961 agtcgtcgag tcgctgccgg atctcgtcga gggggaggtt ccccgaggag accccgaccc
     1021 gcacggtccc gaagaccgtg ctgcccgcgt cgcgtagcgc cgtcacgatc tgcacggtgt
     1081 cggcgtgatc cggatgggcg ggatcctgaa gcccgtcccc caggatcacg gtctcgtact
     1141 gcgcgaactc ggcaatcgcg gcggccaggg aagtcgcgcc gttgatcgcc gagggccggc
     1201 cgtgatacac gaggagctgc ttgggtttca ccgtctcagg ggtatcggcg ggatcgtaga
     1261 ccagatcgag cagtggcccg gcgcccgccg ggagggtcac cgcgaagccg tcggcggtgg
     1321 gcgtggtcgc gacgggactc ccgtccaggc ggacctcgag cggcgcgacc tgggcctcga
     1381 actcgatgtt cccggccgcg ctcccgccac ccaggagcag gctcgcgttc ccgtcctcga
     1441 tgcgcgcgag ctcgaccgtg cggtccgcgt cgagcagatg cagaccgtgc gtgttgtcca
     1501 ggcggacgac ccgcggcttg cgctgattcg agctgtgcgc ggtctgggcg cacagccgat
     1561 cgggggcctg gcgcgtctgc gaggccgcac cgagcaccgc gaactcgggc gcggccctca
     1621 cacatagctt cgtcgtttca tccggccgag cgagcgcgac ggtcgcctca tccaagccgc
     1681 tcgtgaatcc ggaaaacggc ttcgagatga actcgagccg cggctccggc cctgcggcgc
     1741 cgctcccgag ctgcagccgg gcggtcatgc tcgcagcgct cgccggcggt agcacgacgg
     1801 tatctcccgt gaaggcgaag tgcgcagcgc cgtcgatcgt cacggactcg atggagctcg
     1861 ttcccgagga ggtgcgcagg cccatgcccg acagcgcggc tcgctgctcg gcggagagcc
     1921 ccgagaggtc gagcgtcacg atcaggtcac cgctcggaac gtcgatcgac gagctgaaat
     1981 acgtgttgct cgcggcgtgg agtttgtgga tcagctcggc cacgctcggc gcatagacct
     2041 cgttatccgc gaagaaggcg aggtgggcgt cgtagagcgg ctgcgcggtc tcgccgggga
     2101 actgcatcgg aggatcgccc cccatcccgt agtcgtgcca catctcgttg tagagggcgc
     2161 cacgtccgag gctgttctgg aaccagccca ggatctgcgt ctggaggctg gccacctcgg
     2221 tgggcgacca gacccagatg ggatccgtcg tgtagagcca ctggaagtcg ggaacgggcg
     2281 cattcacgac gacgggcttc ggtgccacgc cgggatcgag cgcgaagccc atcacgccgc
     2341 tcgcgtagtt cagggcctgc tcgtagccat gcgtgaagta gagatccgat tcggcaaaga
     2401 cgagcgagta gtcctcgttg cggatcgtcg agccgggatt gatgaagacg cgtggcggag
     2461 tcatcgcgcg gatcggcgcg agctcggcct cgacgatatc gagcgaggcg ccgacctcca
     2521 cgttgaactg ggcgtcgttg aggttcacgc tcatgttgta gtcgtggacg tgggagtggg
     2581 agcccatgag tccgccgagc ttctcgagct gctcgccctt cggccccccg aagagattcc
     2641 aggttctacc gaggttggcg aggctcgtga cgatcgcgag cgccgcatcg accccggtgg
     2701 tctccgcgag ctcgacgagg aaatcgagcg tcggattctg gagatcggcg cgctccgact
     2761 cgtcgtcgtc gacccgggcg atcgccgtca tgccggcgtg cgagagctgc agaccgacga
     2821 cgggctcgtc gggagcgatg agccactgga tggcctcgac catgtagggc aggagcaggt
     2881 tgtcgtagaa gccgagcggt gcggccccga cgttgcgaaa gacgctcgcg gcgcccatcc
     2941 aggtgccgta gcccgagatc acgatgacct tgccgccgga agaggtggtg gtgctcagat
     3001 aggggaacgt caccgtctgg tcgggcgaga tcagctcgag cagcacggcc gac
//
LOCUS       DBNGNK010000059         2751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_941382,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000059 DBNGNK010000000
VERSION     DBNGNK010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_941382"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC150V_01525"
     CDS             complement(<1..617)
                     /locus_tag="AC150V_01525"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3761633.1"
                     /translation="MKVAFCFPGQGSLEAGMGREIAEAVPAAMAVYGIGSEASGLDLQ
                     HLCFEAPLEDLVGTEVQQPALVATSLAILAALRERGIEPDVVVGHSVGEFAALASVGA
                     LDTREAIGLVRERGLAMAEAARNHPGVMAAILGLEDATVETLCRKIIGVWPANYNCPG
                     QIVISGESDAVEECCAEAESLGARRAIRLKVSGAFHSPLVARAADR"
     gene            complement(614..1621)
                     /locus_tag="AC150V_01530"
     CDS             complement(614..1621)
                     /locus_tag="AC150V_01530"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004573078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR3761634.1"
                     /translation="MNVIGSAGRRIGITGLGVNVPERVLTNDELSGIVDTSDEWIRER
                     TGIRERRIASQDEALTDVALPAVRAALADAGVTPREIDLVISATVTPDMLFPTSSAII
                     ADTLDMGDAAAYDLLAGCTGFMYAIAQAYAMLASGLSKRTLVVGGDVLSKLLDWTDRS
                     TLVLFGDGAGAVVMEPVERGGFLGFELGADGGGGEHLWLPGSGSRHFEQTEGLMKMNG
                     REVFKFATRVLVSSATAVLDQCGKDVGDIDVYVPHQANKRIIDYATGKLGIPNEKTIV
                     NVDRFGNTSSGSIPLALEDARRDGLLRDGSLVLMTGMGAGLTWGSALIEWTEGRNGGS
                     S"
     gene            complement(1618..2049)
                     /gene="fabZ"
                     /locus_tag="AC150V_01535"
     CDS             complement(1618..2049)
                     /gene="fabZ"
                     /locus_tag="AC150V_01535"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR3761635.1"
                     /translation="MTPFGRSEIELIIPHREPFLLIDEVVELEPGRRVVARRQVRADD
                     WWFPGHFPERPVMPGVLIVEAMAQAGAVAVLVEEENRGKIAFFAGIDDCRFKRVVEPG
                     DTLTLICEIDQVRGPIGRGKATAHVEEALAARGTLTFAVER"
     gene            complement(2055..2675)
                     /locus_tag="AC150V_01540"
     CDS             complement(2055..2675)
                     /locus_tag="AC150V_01540"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3761636.1"
                     /translation="MQPPLRLRRRIDRELRPAARLDYGRLSDPILVTRANDGDSAALE
                     ALCKRHAPRVRRVASHILRDPEDAGDAAQEALVKMCAHLGQFRGEASFPTWLHRLTVN
                     CCRDAAERRRRRRWEPLEAAEHECADGGPVRASELAELRSELRDALSSLPDSQASVVV
                     LKDAFGLSFGEIADTWGMPVGTAKCYAHRARAGLRERLAGSNGAVA"
BASE COUNT          431 a         1002 c          890 g          428 t
ORIGIN      
        1 cgatcggcgg cacgcgcgac gagcgggctg tggaaggcgc ccgagacctt caggcggatg
       61 gctcgtcggg cgccgaggct ctcggcctcg gcgcaacact cctcgaccgc gtcgctctcg
      121 cccgagatca cgatctgccc ggggcagttg tagttggccg gccagacgcc gatgatcttg
      181 cggcagagcg tctccaccgt cgcgtcctcc aggccgagga tcgcggccat cacgccgggg
      241 tggttccggg cggcctcggc catcgccaga ccgcgctcgc gcacgagccc gatcgcctcg
      301 cgggtgtcca atgccccgac ggaggcgaga gcggcaaact caccgaccga gtgtccgacc
      361 acgacatcgg gctcgatacc tcgctcgcgc agtgcggcga ggatcgccag gctggtagcg
      421 accagagccg gctgctggac ctcggtgccg acgaggtctt cgagcggcgc ctcgaaacag
      481 aggtgctgca agtcgagccc cgatgcctcg ctgccgatcc cgtacaccgc cattgcggcg
      541 ggtacggcct cggcgatctc gcgccccatg ccggcctcga gcgagccctg gcccgggaag
      601 caaaacgcga ccttcatgac gaacctccgt tccttccctc ggtccactcg atcaacgccg
      661 atccccaggt gagccccgcg cccatgccgg tcatgagcac caggctgccg tctcggagca
      721 ggccgtcacg ccgcgcatcc tcgagcgcca gcggaatgga gccagatgac gtgttcccga
      781 agcggtcgac attcacgatt gttttctcat ttgggatgcc gagcttaccg gtcgcgtagt
      841 cgatgatccg cttattcgcc tggtgcggca cgtagacgtc gatgtcaccg acgtccttcc
      901 cgcactggtc gagcaccgcc gtggccgacg agacgagcac gcgggtggcg aacttgaaga
      961 cctcgcgccc gttcatcttc atgagccctt ccgtctgctc gaagtggcgg gagccacttc
     1021 cggggagcca caggtgctcc ccgccgccgc cgtccgcgcc gagctcgaac ccgagaaagc
     1081 cgccccgctc gacgggctcc atcacgaccg ctcccgcgcc gtcaccgaag aggacgagtg
     1141 tcgatcggtc cgtccaatcg agaagcttcg acagaacatc gccgccgacg accagcgttc
     1201 gcttcgacag accagaggca agcattgcgt acgcctgcgc aatcgcgtac atgaagccgg
     1261 tgcagcctgc taggaggtcg taggcggctg cgtcgcccat gtcgagggtg tcggcgatga
     1321 tcgccgagct cgtcgggaac agcatgtcgg gcgtgacggt ggccgagatc acgaggtcga
     1381 tctctcgcgg cgtgacgccc gcgtcggcga gagcggcccg aacggcgggc agcgcgacgt
     1441 cggtcagcgc ctcgtcctgc gacgcgattc gccgttccct gatgccggtc cgctcccgga
     1501 tccactcgtc ggaggtatcg acgatcccgg atagctcgtc gttggtgagc acccgctcgg
     1561 gcacgttcac tcccagcccg gtgatgccga tgcgccgccc cgccgagccg attacattca
     1621 ccgttccacc gcgaatgtca gcgtgccccg tgctgcgagc gcctcttcga cgtgagccgt
     1681 tgccttgcca cgcccgatcg gccctcgaac ctggtcgatc tcacagatca gcgtcagggt
     1741 gtcgccgggc tcgaccaccc gcttgaagcg acagtcgtcg attccggcga agaacgcgat
     1801 cttgcctcga ttctcctcct cgacgagaac cgcaacggct ccagcctgcg ccatcgcctc
     1861 gacgatcagc accccgggca tgaccggccg ctccggaaag tgtccgggaa accaccagtc
     1921 gtcggccctt acctggcgcc ttgccaccac acgccggccg ggttcgagct cgaccacctc
     1981 gtcgatgagc aagaacggct cccgatgggg gatgatcagc tcgatctcac tgcgcccgaa
     2041 gggcgtcacc ggtgtcacgc gaccgctccg ttagagccgg ccagccgctc gcgcagcccc
     2101 gcgcgggctc gatgcgcgta gcacttcgcg gtaccgaccg gcatgcccca ggtgtcggcg
     2161 atctcgccga acgagaggcc gaaagcgtcc ttgagaacga caacactcgc ctggctgtcg
     2221 ggaagcgacg agagcgcgtc ccgcagctcc gatctgagct ccgcgagctc cgaggcccgc
     2281 acgggaccgc cgtcggcgca ctcgtgctcg gccgcctcca ggggctccca gcgcctcctg
     2341 cgacgccgct ccgccgcgtc gcggcagcag ttcaccgtca ggcgatggag ccacgtcgga
     2401 aaggacgcct cgccgcggaa ctgccccagg tgcgcacaca tcttcacgag tgcctcctga
     2461 gcggcgtcgc ccgcgtcctc cgggtcgcgc aggatgtggc ttgcgacccg ccgtacacgc
     2521 ggcgcgtggc gcttgcagag agcctcgagc gctgcgctat ctccgtcgtt ggcgcgcgtc
     2581 accaggatgg ggtcgctcag tcgcccgtag tccagccgag cagcggggcg cagctcacga
     2641 tcgattcgac ggcggagccg cagcgggggt tgcacggcgc gagtctactg ctcggctcct
     2701 cgctcacgcc ccgcgcgaga agcggctcgt gttcccggcg gcgtcccggg c
//
LOCUS       DBNGNK010000060         3163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1084978,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000060 DBNGNK010000000
VERSION     DBNGNK010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1084978"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="AC150V_01545"
     CDS             <1..1680
                     /locus_tag="AC150V_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR3761637.1"
                     /translation="KIVDEFTGRIMEGRRWSEGLHQAVEAKEGVQIREENVTLATITL
                     QNYFRLYEKLGGMTGTAKTEEKEFVEIYGLHVVEIPTNEPIARLDENDFIFKTREAKF
                     AAVVDDIVERHDAGQPILVGTIAVETSEYLSEVLTRQGIPHNVLNAKEHERESEIIID
                     AGQPGAVTIATNMAGRGVDIQLGEGVREVGGLYVLGTERHEARRIDNQLRGRSGRQGD
                     PGETRFYLSAQDDLVRLFAGDRIHGIMNRFKLPDDQPMEASILSRQIEGAQKKVEEQN
                     FVARKNVLKYDDVMNTQRQVIYEQRRRVLEGQDLSGDVEEWIREVVAINVQQFTEAEY
                     PEDWDLGGLVTQMHSLYGTDITVAELREEVELGQEELIEEFTEDALDTYREREEEFTP
                     EVMREIERYVVLTIVDTRWREHLESMDYLREGIHLRAMAQKDPLVEYRGEGHAMFEML
                     GRTIREEVVLTLYHVELAPEQVEQLQPEEQPTSLSYAHETAAGADAIAAAGGAAPAAA
                     GAAAVTASGGAATAVADRPQKPQTVVNERKVGRNDPCWCGSGKKFKKCHGA"
     gene            1831..3078
                     /locus_tag="AC150V_01550"
     CDS             1831..3078
                     /locus_tag="AC150V_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3761638.1"
                     /translation="MARRGLVAGVSIALALAGVLLLAAAYDSTRLIDLDADVAAELRD
                     LPGWITDVAGVVAVLGGYVVLPLAVVAAVVLSVRAGRLNDALFLVVVALGADILVHGG
                     RELHEPTRIDEIGLPEASRFPAGHAATSVAIWAALAFVLARGRSAARRSAVIGAGLAL
                     GLVVASMRVVLDEQALSDVVAGALVGTLWLLLCLFAYEWLGDRQVPPVAAAGAVAALA
                     VLLLAFTYDEPQVVDVDEDVARWVAGELPGWVEWVGRVFSWAGGWIGVVAAGVAMGIV
                     LLRERDWLNLGFLVAVFAGGQLVGNGLKLVFDRARPDFGSAVPLPGSAAFPSGHAVAG
                     VACVGALAVLVTERIESRAVRAAVWVGAVVLGLGIGTSRIALGVHWLTDVLAGWAFGL
                     AWLAVCLLARDAVRRRRTADPIG"
BASE COUNT          489 a         1048 c         1116 g          510 t
ORIGIN      
        1 aagatcgtcg acgagttcac cggccggatc atggagggtc gccgctggag cgaagggctg
       61 catcaggccg tcgaggcgaa ggagggcgta cagatccgcg aggagaacgt cacgctcgcg
      121 acgatcacct tgcagaacta cttccgcctg tacgagaagc tgggtggcat gaccggcacg
      181 gcgaagaccg aggagaagga gttcgtcgag atctacgggc tgcacgtggt cgagatcccg
      241 accaacgagc cgatcgctcg gctggacgag aacgacttca tcttcaagac ccgcgaggcg
      301 aagttcgcgg ccgtcgtcga cgacatcgtc gagcgccacg acgcggggca gccgattctg
      361 gtcgggacaa tcgcggtcga gacgtcggag tacctctcgg aggtgctgac gcggcaagga
      421 atcccacaca acgtgctcaa cgcgaaggag cacgagcggg agagcgagat catcatcgat
      481 gccggacagc caggtgcggt gaccatcgcc acgaacatgg cgggccgcgg tgtggacatc
      541 cagctcggcg agggcgtgcg cgaggtcggc ggactctacg tgctcggtac cgagaggcac
      601 gaggcacgca ggatcgacaa tcagctgcgc ggtcgctccg gccgccaggg cgatcccgga
      661 gagacccgtt tctacctcag cgcccaggac gaccttgtcc ggctcttcgc cggtgaccgg
      721 atccacggga tcatgaaccg cttcaagctt cccgacgacc agccgatgga ggcgtcgatc
      781 ctctcgcgcc agatcgaggg cgctcagaag aaggtcgagg agcagaactt cgtcgcccgg
      841 aagaatgtcc tcaagtacga cgacgtgatg aacacccagc ggcaggtgat ctacgagcag
      901 cggcggcgcg tcctcgaggg ccaggatctc tcgggtgacg tcgaggagtg gatccgcgag
      961 gtggtggcga tcaacgtcca gcagttcacc gaggccgagt acccggaaga ctgggatctc
     1021 ggcggcctcg tcacacagat gcactcgctg tacggcaccg acatcaccgt cgcggagttg
     1081 cgcgaggagg tcgagctcgg gcaggaggag ctcatcgagg agttcaccga ggacgctctc
     1141 gacacctacc gggagcgcga ggaggagttc acgcccgagg tcatgcggga gatcgagcgc
     1201 tacgtcgtgc tgacgatcgt cgacacgcgt tggcgcgagc acctcgagag catggactac
     1261 ctgcgggagg ggatccatct ccgcgcaatg gcgcagaaag acccgctcgt cgagtaccgc
     1321 ggcgaggggc acgcgatgtt cgagatgctc ggtcgcacga tccgcgagga ggtcgtcctg
     1381 accctctatc acgtcgagct cgccccggag caggttgagc agctgcaacc cgaggagcag
     1441 cccacgagcc tgtcctacgc ccacgagacc gcggccggcg ccgatgcgat cgccgcggcc
     1501 ggcggcgctg cgcccgccgc cgcaggagcg gctgcggtca ctgcgagcgg aggagccgcg
     1561 accgccgtcg ccgatcggcc gcagaagccc cagacggtgg tcaacgagcg gaaggtcggc
     1621 cgcaacgacc cttgctggtg tgggtcaggc aagaagttca agaagtgcca cggcgcctag
     1681 ccgtggggga aatcatggtt cccccgcgag cctccgcctt cacggctcca cgctgaccga
     1741 cgttcgtccg gcccccggcc gccgcctgat gcgcatcgac ttctggggcc gccacggtcg
     1801 ccctaccctt gcggatgaga cagttccccg atggcacgtc gtggtctggt cgcaggcgtt
     1861 tcgatcgccc tcgccctcgc cggcgtcctc ctcctcgcgg ccgcgtacga ctccacccgg
     1921 ctgatcgacc tcgacgccga cgtcgcggcc gagctgcgcg acctccccgg ctggatcacg
     1981 gacgtcgccg gcgtggtcgc cgttctcggt ggctacgtcg tcctgccgct cgcagtcgtc
     2041 gctgcggtcg ttctcagtgt ccgcgcgggc cggctgaacg acgcgctgtt cctcgtcgtc
     2101 gttgcgctcg gtgcagacat cctcgtccac ggcgggcgcg agctccacga gccgaccagg
     2161 atcgacgaga tcggtctccc ggaggcgtcg aggttcccgg ccggccatgc ggcgacctcg
     2221 gtcgcgatct gggctgcact cgccttcgtg cttgcccggg ggcgatctgc cgcccggcgg
     2281 tcggcggtta tcggcgcggg tctggcactc ggcctcgtcg tagcctccat gcgcgtcgtg
     2341 ctcgacgagc aggctctgag cgacgtcgtg gcgggagcgc ttgtggggac gctctggctg
     2401 ttgctctgcc tcttcgccta cgagtggctc ggcgatcgtc aggttccgcc ggtcgcggct
     2461 gccggagcgg ttgcggcact cgcggtcctc ctactcgcat tcacatacga cgagcctcag
     2521 gtcgtcgacg tcgacgagga cgtcgcccgg tgggtcgccg gcgagctgcc ggggtgggtg
     2581 gagtgggtcg gcagggtctt ctcgtgggcc ggtggctgga tcggcgtcgt cgccgcgggg
     2641 gtggcgatgg ggatcgtcct gcttcgagag cgggactggc tcaatctcgg gtttctcgtc
     2701 gccgtcttcg cgggcggtca gctcgtgggc aacggcctca agctcgtctt cgaccgggcc
     2761 cgccccgact tcgggagcgc cgtgccgctt cccggcagcg ccgctttccc gtcgggtcac
     2821 gccgttgccg gcgtggcatg cgtcggcgcg ctcgccgtcc tggtcacaga gcggatcgag
     2881 tcccgcgccg tccgcgcggc ggtgtgggtc ggagccgtcg tcctaggcct cggcatcggc
     2941 acgagccgta tcgcgcttgg agtgcactgg ctcaccgacg tgctcgcggg ctgggcattc
     3001 ggcctcgcct ggctggccgt ttgcctcctt gctcgggatg ccgttcgacg ccgcaggaca
     3061 gccgacccga tcggctagca tcctggcgtg gccgagacga cacagagcct cgaccagcag
     3121 ctcggggaga tcagggtcca gctcgcctgg gtccgtgact acc
//
LOCUS       DBNGNK010000061         2503 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_654701,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000061 DBNGNK010000000
VERSION     DBNGNK010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_654701"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="AC150V_01555"
     CDS             complement(<1..538)
                     /locus_tag="AC150V_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR3761639.1"
                     /translation="MTRRVAAVVVVAALAVVGIAAGCSGSDDAPATTQAQPPPPPRPL
                     RIIFPEGFTRKEMADRVAAVRKIAIAERGITPRLSKPGYVAAAAVAKPPPAFRKDWKK
                     GVIEGFLFPATYDFTRLTTARRFVAEQLGTFRRMWAKVDLRYARSKNLTPYDVLIIAS
                     MIEEEAAVPAERRLVAAVI"
     gene            complement(535..921)
                     /gene="ruvX"
                     /locus_tag="AC150V_01560"
     CDS             complement(535..921)
                     /gene="ruvX"
                     /locus_tag="AC150V_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR3761640.1"
                     /translation="MALDYGSARTGVAVSDATGTIATPAGVVEDAASEQGLGRIDALV
                     REHGVRRVVVGMPYTLSGEKGSQAIETESFVAALGKAIDVPVETYDERFTTTLAGLTI
                     SDAPEDALAAAHLLAGYLEWAGTRGE"
     gene            complement(927..>2503)
                     /locus_tag="AC150V_01565"
     CDS             complement(927..>2503)
                     /locus_tag="AC150V_01565"
                     /inference="COORDINATES: protein motif:HMM:NF013570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR3761641.1"
                     /translation="AIGLSELWRISDVVVAQMGYWYPELRADTASIGAVLRTEEERFS
                     ETLERGMKVFEDLAGRDAISGEDAFTLAATYGFPLELTVELAEERGQPVDVDGYRIEM
                     ERHREISRAGGETEVQRAAEFARESGFQSEFVGYAKTEVLTQLGALEELDDGLFLAKL
                     RESPFYPAGGGQVTDAGVVELDDERETVAELRDAYRLEDDQVLLLAGAGFSPGDRVRA
                     VVPWGVRFPTMANHTATHLLHRALREVLGEHVKQAGSAVRPDKLRFDFTHEHALTDEE
                     RDRVERRVNEKIFENLPVRAFETPIDEARRLGTTMLFGEKYGDIVRVVEIEGYSRELC
                     GGTHVRSTAEIGPFVILSEGSVGAGARRIEAITSGEAWAHLHGQARTLEEVRAEAAEL
                     RTELKKKGTAARQDAPAVTAQVRTEGGVNVIVQSVEGLDADALLDLSDRFKQAHAPAA
                     VVLGSKEDGRVHLVANFDDSVADRVSASDVVRGAAALVGGGGGGRPTMARAGGKDPER
                     LDDALAEAEKLILEAL"
BASE COUNT          367 a          920 c          819 g          397 t
ORIGIN      
        1 agatgacggc cgccacgagg cgccgctcgg cgggcaccgc ggcctcctcc tcgatcatcg
       61 acgcaatgat cagcacgtcg tacggcgtga ggttcttcga cctcgcgtag cggaggtcga
      121 ccttggccca catccggcgg aacgtcccga gctgctcggc gacgaagcgc cgagcggtcg
      181 tgaggcgggt gaagtcgtac gtcgcaggga agagaaagcc ctcgatgaca cccttcttcc
      241 agtccttccg aaatgccggc ggcggttttg cgaccgccgc cgcggccacg tagccgggct
      301 tcgacaggcg gggcgtgatg ccgcgctccg cgatcgcgat cttccgcacc gccgcgacgc
      361 gatcggccat ctctttccgt gtgaaaccct ccgggaagat gatccgcaac ggccgcggcg
      421 gcgggggcgg ctgggcttgc gtcgtcgccg gcgcgtcgtc gctcccgctg cagccggccg
      481 cgatcccgac gacggcgagg gcggcgacca cgaccaccgc ggcgacgcga cgggtcactc
      541 gcctcgcgtg cccgcccact cgaggtaccc ggcgagcaga tgcgcggcag ccagcgcgtc
      601 ctcgggcgcg tccgagatcg tgagcccggc gagcgtcgtc gtgaaacgct cgtcgtatgt
      661 ctcgaccggc acgtcgatgg ccttgccgag ggcggccacg aacgactcgg tctcgatcgc
      721 ctgcgacccc ttctcgcccg agagcgtgta gggcatgccg acgacgacgc gccggacgcc
      781 atgctcgcgg acgagagcgt cgattcgccc gagcccctgc tcgctcgccg cgtcttccac
      841 gaccccggcg ggtgtcgcga tggttccggt cgcatctgac accgcgacgc cggtgcgcgc
      901 ggatccgtag tcgagtgcca tgatcttcac agtgcttcga ggatgagctt ctcggcctcc
      961 gccagcgcgt cgtcgagccg ctcggggtct ttcccgcccg cgcgggccat cgtcggccgc
     1021 cccccgccgc cgccaccgac gagcgccgcc gccccgcgca cgacgtccga cgcgctgacg
     1081 cgatccgcga ccgagtcgtc gaagttcgcg accagatgca cgcggccgtc ctccttcgac
     1141 cccagcacga cggccgcagg agcatgcgcc tgcttgaagc gatcggacag atcgagcagc
     1201 gcatcggcgt cgagcccctc gaccgactgg acgatcacat tgacgccgcc ctcggtacgc
     1261 acctgcgcgg tgacggcggg agcatcctgc cgcgccgcag ttcccttctt cttgagctcc
     1321 gtacgcaact ccgccgcttc ggcgcgtacc tcctcgagcg tgcgcgcctg gccgtggaga
     1381 tgggcccagg cctcgcccga ggtgatcgcc tcgatccggc gggcgccggc gccgacggat
     1441 ccctcggaga ggatcacgaa cggaccgatc tcggccgtcg agcgcacgtg cgtgcccccg
     1501 cagagctcgc gcgagtaccc ctcgatctcg acgacacgga cgatgtcgcc gtacttctcg
     1561 ccgaagagca tcgtggttcc caggcgacgc gcctcgtcaa tgggtgtctc gaaggcgcgc
     1621 acggggagat tctcgaagat cttctcgttg actcgccgct cgacgcggtc gcgctcctcg
     1681 tcggtcagcg cgtgctcgtg ggtgaagtcg aagcggagct tgtcgggccg aacggccgat
     1741 ccggcctgct tgacgtgctc gccgagaacc tcccgcagcg ctctgtgcag gaggtgggtc
     1801 gccgtgtggt tggccatcgt cggaaaacgg acgccccagg gcacgacggc ccgcacacga
     1861 tcacccggcg agaaccccgc acctgcgagc aacagcacct gatcatcctc caaccggtat
     1921 gcgtcgcgga gctcggcgac cgtctcacgc tcgtcgtcga gctcgaccac gcctgcgtcc
     1981 gtgacctggc cgccgccggc cgggtagaac ggcgactccc tcagcttcgc gaggaagagc
     2041 ccatcgtcga gctcttccag cgcgccgagc tgggtcagca cttcggtctt cgcgtacccg
     2101 acgaactcgc tctggaaccc gctctcgcgc gcgaactcgg cggcccgctg cacctcggtc
     2161 tccccgcccg ctcgtgagat ctctcggtga cgctccattt cgattcgata accgtcgacg
     2221 tcgacgggct gaccgcgttc ctcggccagc tcgacggtta gctcgagcgg gaagccgtat
     2281 gtggcggcca gcgtgaatgc gtcctcgcct gagattgcgt cgcggccggc gagatcttcg
     2341 aacaccttca tgccgcgctc gagcgtctcg ctgaatcgct cttcctccgt tcgcagcacg
     2401 gccccgatcg aagcggtgtc cgctcgcagt tccggatacc agtagcccat ctgcgcgacg
     2461 acgacgtcgg agatgcgcca gagctcggag aggccgatcg cac
//
LOCUS       DBNGNK010000062         2822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1229052,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000062 DBNGNK010000000
VERSION     DBNGNK010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1229052"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC150V_01570"
     CDS             complement(<1..512)
                     /locus_tag="AC150V_01570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3761642.1"
                     /translation="MLPREVEYARPATIDEAIGLLSAHDGAIPLAGGQTLINVMKARV
                     ASPEALVDLGDLDELRTISFSDDGTLELGAMVTLTQLVASSEVEVARAILAEVAETVA
                     DAQVRNRGTVGGNVCVNDPTNHLPPLLVALGAEMTILGEGGTRVVPADEFFIGVYMTA
                     VGPGELLTRIS"
     gene            576..2216
                     /locus_tag="AC150V_01575"
     CDS             576..2216
                     /locus_tag="AC150V_01575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015435488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3761643.1"
                     /translation="MAFDAVFVGSGINSLTGAALLAKSGWSVCVLEREDRLGGCIRTV
                     TDLTEPGFTHEVLSSWHPLFTGSAAYAELEGDLGRHGLEYVNTELPTASAYPDGSSMF
                     LLTTMEANRAEFDRHAQGDGDAWQEQFETFMADADLSFGVLTTELWSPGGLGLARRLI
                     SRFGRDGALSFAGRALVTCRDWVTATFESEQAHGLLAPWVLHTGLGPESALSGFMTQV
                     IGCAVQLGGMPVPVGGGRRLVEALAAIVGENGGELRVDADVERVLVSAGRATSVRLVG
                     GETIQAARAVVASVTPAQLYGRLLGEGEADPDVVEAAGRYRFGRGGMQIHIAMDEPPT
                     WYGDERLAETAIVHITPGLDGVSRAVNEAERGLLPAEATIVVGQPCAVDATRAPAGNW
                     ILWLQLQELPAGRVQGDAGGEIDVGGGEWSEALREAYADRIVSRLGRHITNLERATKK
                     RVCFSPTDIEGLNCNLVNGDIYAGSCALDQNLLWRPLPEAPGHATSVESLYHIGASTH
                     PGPGLGAGSGYLVAKQLTKPALPKRLLGRVPVARHYVT"
     gene            2230..>2822
                     /locus_tag="AC150V_01580"
     CDS             2230..>2822
                     /locus_tag="AC150V_01580"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3761644.1"
                     /translation="MTTLLPTGTVTLLFTDIEGSTRLLHELGPKAYAEALADHRGQLR
                     SVFAEHGGVEVDTQGDAFFAAFPTAPDAAAAAVAGQVALAAGPIRVRVGLHTGTPTVT
                     QEGYVGDDVHRGARVAALAHGGQVLVSSATAALLGSAPLLDLGLHRLKDFDAPARLYQ
                     LGEGAFPSLRTPGSIELPTPATRFLGRERELFDAVST"
BASE COUNT          421 a          998 c          978 g          425 t
ORIGIN      
        1 gagatcctcg tcaggagctc gccgggcccc acggccgtca tatacacgcc gatgaagaac
       61 tcgtccgctg gaacaacgcg ggtgccacct tctccgagaa tcgtcatctc cgcaccgagc
      121 gcgaccagaa gcggcggcag gtggttggtc ggatcgttga cgcacacgtt cccacctacc
      181 gtgccgcggt tccggacttg cgcatccgcg accgtctcgg ccacctcggc cagaatcgcg
      241 cgtgccacct cgacttcgga cgatgcgacg agctgggtga gcgtcaccat cgcgccgagc
      301 tcgagagtgc cgtcgtccga gaacgaaatg gtgcgcagct cgtccagatc gccgaggtcg
      361 acgagggcct cgggggacgc cactcgggcc ttcatcacgt tgatcagggt ctgaccgccg
      421 gcgagcggga tcgcgccgtc gtgcgccgag aggaggccga tcgcctcgtc gatcgtggcc
      481 ggacgtgcgt attcgacctc acgcggaagc atgcctcgaa cctcgcacac gggcgcgtga
      541 catgtcaaga gagaacccgt ggtacgctcg ccgccgtggc cttcgacgcg gtcttcgtcg
      601 gctccgggat caactcgctg accggggcag cgctcctggc gaagtccggc tggagcgtct
      661 gcgtgctcga gcgcgaagac cggctcggcg gctgcattcg aaccgtcacc gaccttaccg
      721 agcccggttt cacgcacgag gtgttgagct cgtggcatcc gctcttcacc ggctcggcag
      781 cctacgccga gctcgagggc gacctcggtc gtcacggact cgagtacgtg aacacggagc
      841 tccccaccgc ctctgcttac ccggatggat cgagcatgtt cctcctcacg acgatggagg
      901 cgaaccgcgc cgagttcgac cgccacgccc agggcgacgg cgacgcgtgg caggagcagt
      961 tcgaaacctt catggccgac gcagacctca gcttcggcgt gctcacgacc gagctgtggt
     1021 cgccaggcgg gctcggactc gcgcgacggc tcatctcccg cttcggccgg gacggggcgc
     1081 tgagcttcgc cggacgggcg ctggtcacgt gccgcgactg ggtgacggcc acgttcgagt
     1141 cggagcaggc gcatgggctg ctggcgccgt gggtgctgca caccgggctc gggcccgaga
     1201 gcgcgctgtc cggcttcatg acgcaggtga tcggctgcgc ggttcagctc ggcggcatgc
     1261 cggtgcctgt cgggggtggc cgccggctgg tcgaggcgtt ggctgcgatc gtcggcgaga
     1321 acggcggcga gcttcgcgtc gatgcagacg tcgagcgtgt cctcgtctcc gcaggacgcg
     1381 cgacttccgt tcggctcgtg ggcggcgaga cgatccaggc ggcgcgagcc gtggtggcgt
     1441 ctgtgacccc ggcgcagctc tacgggcgcc tgctcggcga gggcgaagcc gacccggacg
     1501 tcgtggaggc tgccggccgg taccgcttcg gacgtggcgg gatgcagatc cacatcgcaa
     1561 tggacgagcc tcccacctgg tacggcgacg agcgcctcgc cgagaccgcc atcgtccaca
     1621 tcacgccggg tctcgacgga gtctcgcggg ccgtgaacga agcagagcgc ggcctgctgc
     1681 ccgcggaggc gacgatcgtg gtcggccagc cctgcgcggt cgacgcgacg cgggcgccag
     1741 ccggaaactg gatcctctgg ctccagttgc aggagcttcc cgccgggcgc gttcagggcg
     1801 acgccggcgg cgagatcgac gtcggcggcg gcgagtggtc ggaagccctg cgcgaggcgt
     1861 acgccgaccg gatcgtcagc cgcctcgggc gccacatcac gaacctcgag cgggccacca
     1921 agaagcgcgt gtgcttttcc cccacggaca tcgagggact gaactgcaac ctcgtgaacg
     1981 gggacatcta tgcgggctct tgcgcactcg accagaacct gctctggcgc cccctgcccg
     2041 aggcgccggg gcacgcgacg tccgtcgaaa gcctgtacca catcggggcg agcacgcatc
     2101 cgggcccggg gctgggggcc ggctccggct atctggttgc gaagcagctc acgaaaccgg
     2161 ccctccccaa gcgcctgctc gggcgcgtcc ccgtcgcccg ccactacgtg acgtgaccgc
     2221 gtcgctcatg tgaccaccct gctaccgacc ggaacggtca cccttctctt cacggacatc
     2281 gagggctcga ctcggctgct gcacgagctg gggccgaagg cctacgcaga ggcgctcgcc
     2341 gaccaccgcg ggcagctccg gtctgtcttc gcagagcacg ggggagttga ggtcgacacg
     2401 cagggtgacg ccttcttcgc cgccttcccg accgcccccg acgccgccgc cgcggccgtc
     2461 gcaggacagg tagctctcgc ggcggggccg atccgcgtcc gagtaggact gcacacgggt
     2521 accccgacag tcacgcagga aggctacgtc ggcgacgacg tgcaccgggg cgcgcgggtc
     2581 gcggcgctcg cgcacggcgg ccaggtactc gtctcgtcgg cgaccgcagc gctgctgggc
     2641 agcgcgcctc tactcgacct gggcctgcat cgcctcaagg acttcgacgc tccggcccgc
     2701 ctctatcagc tcggcgaagg cgccttcccg agcctccgta cgccgggcag catcgagctg
     2761 cccacccccg ccactcgctt tctggggcgc gaacgcgaac tcttcgacgc ggtgtcgacg
     2821 tg
//
LOCUS       DBNGNK010000063         9040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_942203,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000063 DBNGNK010000000
VERSION     DBNGNK010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_942203"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC150V_01585"
     CDS             <1..203
                     /locus_tag="AC150V_01585"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3761645.1"
                     /translation="PIEVDRQEVQRLHAAGAQLVDVLPADEYADEHIAGAVNIHLKRL
                     DRETTAGLDRNRPVIVYCHDSV"
     gene            207..689
                     /locus_tag="AC150V_01590"
     CDS             207..689
                     /locus_tag="AC150V_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3761646.1"
                     /translation="MSPRAAWRLESLGFEQVFDYVAGKADWGAAGLLLEGANGSETRA
                     GAHVRTDVPACLLGDSLAAVRRESEQAGWDTCFVVNQEGIVLGRLGRQALSRDDDVPV
                     EEAMTLGPSTVRPDAGLERLARRMRKQNLTGLPVTTSDGRLVGLLRLEDAERAFDRPR
                     "
     gene            974..1873
                     /locus_tag="AC150V_01595"
     CDS             974..1873
                     /locus_tag="AC150V_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761647.1"
                     /translation="MSLRPFVVLTALCALVLPAGAGASELIDRNAKNVALEVNAKGEA
                     LLSYVKGGQDKHVLVWGAVNAIHPTKTRRQVEFKVDYTGGWGKYRDAKYWQNFKNACR
                     PYSGPKLDWFVTACTAPDGTHWAVQAWQRVLPNYGVKPSADRAVWELRLSHFDGVLPK
                     LEIQMNWAYKKFDHLFGRFTYKGTPVHGFGSTPKGEPLDSFGRNLYVDTFNSAYGPGW
                     MRDNGFLTHQGNGKFCYGFYKHGPHPSGMGERYRATIIGPGVTPDVIWVGDSLGAYDQ
                     ERDLALHEQQKQFFRDDPLCKPV"
     gene            1920..2591
                     /locus_tag="AC150V_01600"
     CDS             1920..2591
                     /locus_tag="AC150V_01600"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018501928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3761648.1"
                     /translation="MDATPPMIEAVVFDLDGVVVDTEHIWDAVREELTRERGGRWHEG
                     AQAAMMGMSSTEWSQYLTGVIGLAGTPEEINAEVVRRMLERYEQELPVIEGAVEAVRR
                     LAGEFELAVASSSNRPLIEAVLEGAGIADLFAATVSSEEVARGKPSPDVYLEAAKRLN
                     APPRRCAAVEDSASGIRAANAAGMRVIVYPNRMYPPAPDALALAAVELASLDELTAET
                     VRGGG"
     gene            complement(2601..3830)
                     /gene="fabF"
                     /locus_tag="AC150V_01605"
     CDS             complement(2601..3830)
                     /gene="fabF"
                     /locus_tag="AC150V_01605"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3761649.1"
                     /translation="MRRIAVTGLGAVTPLANDAPATWDAAVAGRSGIDWIQSFDASDF
                     PVRIGGEVKGFDPAGVVSPKEARRLERNVLLAVAAAREAWSDAGIDDFDSSRTGILVG
                     SAIGGVTGVLAQAEVLVDRGPSRLSPHFLPNVLVDSASGQIAIELGLRGPNYAPVSAC
                     ATGSHAVGEAAEMIRRGDADIVLAGGTESAWHPVILGGFCAMRGLVAEEEDPTLASRP
                     FDATRAGFVMGEGACILLLEELERSRARGATVYAEVLGYGASNDAHHMAQPDPDSVGV
                     AEMMRAALERTGVAPERVGYINAHGTSTPLGDLAETKAIKDVFGAHAYELAVSSTKSV
                     TGHCFGAAGAVEAMMCVLALHHGVLPPTINYRKPDPECDLDYVPNEARRVEVDVALSN
                     AMGLGGHNGCVLLGRVD"
     gene            3859..4911
                     /locus_tag="AC150V_01610"
     CDS             3859..4911
                     /locus_tag="AC150V_01610"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR3761650.1"
                     /translation="MARTRGGKPAERPAELDRSIPFPPMEAELVRDLPEGDGWQFEPK
                     WDGFRGVLENLSGELRLWSRNGRPLLRYFPELGPLGDLLPARSALDGEIVIARDGVLD
                     FDAMQTRLHPAESRVRKLAAEIPADFIAFDVLIWAGDELWHAPLAERRRALEKKAGGF
                     HLSPATGNREAAVAWLEDFEAAGLDGVIAKRLDAEYRPGSRDAVAKVKPEKTADCVVV
                     GLRWKSKPDRIATLLLGLYGEDGELDYVGSTAVAARRHADIAEQLVPLLEDAPERRFS
                     EPNRWGGGELEEAPVRPELVVEVRYDKVQGNRFRHGTKLLRFRADKAPSDCTWRELRP
                     PRSPDDPTFESLLGTA"
     gene            complement(4918..5793)
                     /locus_tag="AC150V_01615"
     CDS             complement(4918..5793)
                     /locus_tag="AC150V_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3761651.1"
                     /translation="MAAVVLSLVSAVLFGAYSVAIRWGQARVPDLDAGVGAVITIASV
                     AILGVAGVGLAVTGDSLALGELWPYVLIGLAVPGTTQFLYQRSVRAAGAARPSIVIGT
                     APVLSVGIALVFLGEPLTWPVLVGTAFIVGGTTLLARERLRPEHIRRIGLVLAAVCAL
                     LFAFRDNAVRLAADHVDVPSLQAGAAPLAAGAVAGWLILASASRRELLVRVRASLKPM
                     LPAALVFAGALGTLVVAFTEGRVTVVAPLNATQALFAVAFAHLAFRTSERIGARVALA
                     ALLVVAGAVIVGALR"
     gene            complement(5822..6835)
                     /gene="ligD"
                     /locus_tag="AC150V_01620"
     CDS             complement(5822..6835)
                     /gene="ligD"
                     /locus_tag="AC150V_01620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR3761652.1"
                     /translation="MADSPFIELEVGERVVKVTNPDKVLFPARSETKRDLVEYYLAVG
                     DGIVRALYERPTQLRRFPDGVEGEMIYQKRVPKHRPDWVGTARVTFPSGRHADELCVT
                     ELAQVAWAANLAVVDFHPWPSRRTDTEHPDELRIDIDPQPGTHFGDAKHVAGIVRETL
                     AEIGYTGWPKTSGNRGIHVACRIEPSWEFPVVRRAALALAREIERRAPRAVTTAWWKE
                     ERGKRVFIDYNQNARDRTIASAYSVRGRADATVSAPVGWDELPDVETEDFTLATMPGR
                     FAELGDVQAGIDEAVCDLRVLLEWVERDETEGLGEAPYPPNFPKMPDEPKRVQPSRAR
                     KDT"
     gene            6949..7833
                     /locus_tag="AC150V_01625"
     CDS             6949..7833
                     /locus_tag="AC150V_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3761653.1"
                     /translation="MRKALAISLAVAAAAALTVAASATGGGSAAAATGAVPGCAVSGL
                     NLLKEGELTIGADNPAFPPWFGGAEKDPWKVSNPFSRKGYESAVAYAIARELGFRNSA
                     VDWTFVPFSKSFRPGKKPFDFYVTQVSITKERAKVVDFSKPYYFVNQAIVGRKGTQIA
                     KAKSIKDLKPYRLGAQLGTTSYAFITRNIKPSQTPRAFDTNDLAVQALKNGQIDGIVV
                     DLPTSFYVTAVQVEDGKIVGQFQNKGGKEGFGLVFEKGNKLRACVNKAIDRLWKKGTI
                     AKLQQAWLSRVAGAPVVK"
     gene            7940..8683
                     /locus_tag="AC150V_01630"
     CDS             7940..8683
                     /locus_tag="AC150V_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3761654.1"
                     /translation="MAIVLLVTNSPGWEEFKAYFFDWDKYKESFPEIAEAFLLNIRIF
                     LIAEAIILVTALILAVLRGLPGPVLFPVRAFAVVYADFFRGVPTILVILMLGPGLPAL
                     GVEGIPTDPVFWAVVSLVLVYTAYVSEVYRAGIESVHPSQSAAARSLGLSHMQSMRYV
                     IIPQAVRRVIPPLLNDFIGLQKDTALVSVLGVVEVLRQTQINEAATFNGTYYLVAATL
                     FVALTIPLARFTDWLVARDRKRRQAAATT"
     gene            8680..>9040
                     /locus_tag="AC150V_01635"
     CDS             8680..>9040
                     /locus_tag="AC150V_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3761655.1"
                     /translation="MKAPAEALRVEGVWKSFDKLEVLRGIDLVLAEHEVVCMIGASGS
                     GKSTLLRCVNLLETIDDGRIVLHGEEITGQGVNVDAIRRGIGIVFQAFNLFPHMSVLR
                     NVTLAPRKALGLSKKAAE"
BASE COUNT         1446 a         3155 c         3089 g         1350 t
ORIGIN      
        1 tgcccatcga ggtcgaccgc caggaggtcc agcgcttgca cgcagccggc gcgcagctcg
       61 tcgatgtgct tccggccgac gagtacgcgg acgagcacat cgccggcgcc gtcaacatcc
      121 atctcaagcg gctcgatcgg gagacgacgg ccggactcga ccgcaaccgg ccggtgatcg
      181 tctactgtca cgactccgtg tgagacatga gcccgcgggc cgcgtggcgg ctcgagagcc
      241 tcggcttcga gcaggtgttc gactacgtcg ccggcaaggc cgactggggc gccgccggtc
      301 tcctactcga gggagccaac ggcagcgaga cgagggccgg cgcgcacgtc cgcaccgacg
      361 tgccggcctg cctcctcggc gactcgctgg cagccgtccg ccgggagtcg gagcaggccg
      421 gctgggacac ctgcttcgtc gtcaaccagg aggggatcgt gcttggccgg ctggggcggc
      481 aggcgctgag ccgtgacgac gatgtccccg tcgaggaggc gatgacgctg ggcccgagca
      541 ccgtccgccc cgacgccggg ctggagcgac tcgcccgacg catgcgcaag cagaacctga
      601 ccgggttgcc cgttaccacc tccgacgggc gactcgtcgg actcctccgg ctagaggacg
      661 ccgagcgggc gttcgatcgc cctagatagc cgcgttctgc cgcttcggac cgctccgcaa
      721 cctgagcgta atcatccgcg ccggcgcgag ctccgtaaca cctaaaggcg cctttctcat
      781 gactggcgcc aacttggggt cctgcggggg agagccactc ggcctctccc ccgcgctcgt
      841 tcgagggagt ggaacctgca cacccgtccc tcgttcgagc gaccgccgag ctcgaaatca
      901 ctcgtccaag gggccgccct ccccttttgg cggggtggaa gcgaggccgc cccctcctga
      961 cgctgcgaga gcgatgagtt tgcggccctt cgtcgtcctt accgcccttt gcgcgttggt
     1021 gctgccggcg ggcgccgggg cctccgagct gatcgaccgg aatgccaaga acgtcgcgct
     1081 cgaggtcaat gcgaaaggcg aagctctgct cagctacgtg aagggcggcc aggacaagca
     1141 cgtgctcgtc tggggcgcgg tgaacgcgat ccacccgacg aagacgcgcc ggcaggtgga
     1201 gttcaaggtc gactacaccg gcggctgggg caagtaccgt gacgcgaagt actggcagaa
     1261 cttcaagaac gcctgccggc cctactccgg ccccaaactc gactggttcg tgacggcatg
     1321 caccgccccg gacggaacgc actgggccgt tcaggcctgg caacgcgtgc tgccgaacta
     1381 cggcgtgaag ccgagcgccg accgtgcagt ctgggagctg cggctctcgc acttcgacgg
     1441 cgtgctgccg aagctcgaga tccagatgaa ctgggcgtac aagaagttcg accacctgtt
     1501 cggccgcttc acgtacaagg ggacgccggt gcacggcttc gggtcgactc ccaagggcga
     1561 gccgctcgac tccttcggca ggaatctcta cgtcgacacg ttcaactccg cctacggccc
     1621 gggctggatg cgtgacaacg gctttctcac gcaccagggc aacggcaagt tctgctacgg
     1681 cttctacaag cacgggcctc acccctccgg aatgggcgag cgctaccggg cgacgatcat
     1741 cggcccgggt gtgactcccg acgtgatatg ggtgggcgat tcactcggcg cgtacgacca
     1801 ggagcgcgac ctcgcgctgc acgagcagca gaagcagttc ttcagggacg accctctctg
     1861 caagcccgtc tgagcacgtc tgcttgacgg ccgccgcgcg cgaggcttga ctgcccgccg
     1921 tggacgcgac cccgccgatg atcgaagcgg tcgtcttcga cctcgacggg gtcgtcgtcg
     1981 acaccgagca catctgggac gcggtgcgcg aggagctcac ccgggagcga ggcgggcgct
     2041 ggcacgaagg cgcgcaggcg gcaatgatgg gcatgagctc gaccgagtgg tcgcagtacc
     2101 tgaccggggt catcggtctg gccggaacgc cggaggagat caacgcggag gtcgtgcggc
     2161 ggatgctcga gcgctacgag caggagctgc cggtgatcga gggcgcggtc gaggccgtgc
     2221 gcaggctcgc gggcgagttc gaactggccg tcgcctcctc gtcgaaccgc ccgttgatcg
     2281 aagcggtgct ggagggcgcc ggaatcgccg atctcttcgc cgcaaccgtc tcctccgagg
     2341 aggttgcgcg cgggaagccc tcccctgacg tctacctcga ggcggcgaag cgcctcaacg
     2401 cgccgccgag gcgctgtgcc gccgtcgagg actcggcgag cggcatccgc gccgcgaacg
     2461 ctgcgggaat gcgcgtgatc gtttacccga accgcatgta cccgcccgca cccgacgcgc
     2521 tggccctcgc cgcggtcgag ctcgcctcgt tggacgagct gaccgccgag accgttcgag
     2581 gcggcggcta gcggcgaacg ctagtcgact cgaccgagaa ggacgcaccc gttgtggccg
     2641 ccgagtccca tcgcgttcga gagcgccacg tcgacctcca cgcgtcgcgc ctcgttgggc
     2701 acgtagtcca ggtcgcactc cgggtcgggc ttgcggtagt tgatcgtcgg cggcagcacg
     2761 ccgtggtgga gcgccagcac gcacatcatc gcctcgaccg cgcccgccgc gccgaagcag
     2821 tgaccggtca ccgacttcgt cgaggagaca gcgagctcgt aggcgtgcgc gccgaagacg
     2881 tccttgatcg ccttcgtctc ggcgaggtcg cccagcggag tcgacgtccc gtgggcgttg
     2941 atatagccga cccgctcggg cgccacgccg gtccgctcga gcgcagcgcg catcatctcg
     3001 gccaccccga ccgagtccgg atcgggctgc gccatgtggt gcgcgtcgtt cgaggcgccg
     3061 taacccagga cctcggcgta gaccgtcgct ccgcgcgcgc gggaacgctc cagctcctcg
     3121 agtagaagaa tgcacgctcc ctctcccatg acgaatcccg cgcgggtcgc gtcgaacggg
     3181 cgcgaggcga gcgtcgggtc ttcttcctcg gcgacgaggc cgcgcatggc gcagaagccg
     3241 ccgaggatga ccgggtgcca ggccgactcg gtgccgcccg cgaggacgat gtcggcgtcg
     3301 ccgcgacgga tcatctccgc cgcctcgccg acggcgtgtg accccgtcgc gcaggccgag
     3361 accggcgcgt agttcgggcc gcggaggccg agctcgatcg cgatctgacc ggatgcggaa
     3421 tcgacgagca cgttcgggag gaaatggggc gacaggcggc tcgggccgcg gtcgacgagc
     3481 acctccgcct gcgccaggac gcccgtgact ccgccgatcg ccgagcctac gagaatcccc
     3541 gtgcgagagc tgtcgaagtc gtcgatgccc gcgtcggacc aggcttctct tgccgccgcg
     3601 accgcgagca ggacgtttcg ctcgagccgg cgtgcctcct tgggcgagac gacgcccgcc
     3661 gggtcgaagc ccttcacctc gcctccgatc cgcaccggaa agtcgctcgc gtcgaagctc
     3721 tggatccagt cgatgccgct ccggcccgcg accgccgcgt cccaggttgc cggagcatcg
     3781 ttcgccagcg gcgtaaccgc tcccagaccg gtaacggcga tgcggcgcat tgattcgaga
     3841 gcgtagtagt ttgccgacgt ggcgcgtaca cgaggcggca agccggccga acgcccggcg
     3901 gagctcgatc gctcgattcc attcccaccg atggaggccg agctggtgcg cgacctcccc
     3961 gagggcgatg gctggcagtt cgagccgaag tgggacggct tccgcggcgt actcgagaac
     4021 ctctccggtg agctcaggct ctggtcgcgc aacgggcgtc cgttgctgcg ctacttcccg
     4081 gagctcggcc cactcggaga cctgcttccg gcccggtccg ccctcgacgg cgagatcgtc
     4141 atcgcgcggg acggggtgct cgacttcgac gccatgcaga cgcggctgca tcctgcagag
     4201 agccgggtgc gaaaactcgc cgcagagatc ccggccgact tcatagcctt cgacgtgctc
     4261 atctgggccg gcgacgagct gtggcacgcc ccgctagcgg agcggcgtcg tgccctggag
     4321 aagaaggctg gcggctttca cctctcacct gcgaccggga atcgcgaggc cgccgtcgcc
     4381 tggctcgagg acttcgaggc ggccgggctc gacggcgtaa tcgccaagcg gctcgacgcc
     4441 gagtaccggc ccgggtcgcg cgacgcggtg gcgaaggtca agccggagaa gaccgccgac
     4501 tgcgtcgtcg tcgggctgcg ctggaagtcg aagccggacc ggatcgcaac gttgctgctc
     4561 ggcctctacg gcgaggacgg cgagctcgac tacgtcggct cgaccgccgt cgcggccagg
     4621 cgccacgccg acatcgcgga gcagctcgtg cccctgctcg aggacgcccc cgagcgccgc
     4681 ttctccgagc ccaaccgctg gggcggcggc gagctcgagg aggcgcccgt acggccggag
     4741 ctcgtcgtcg aggtccgcta cgacaaggtg cagggaaacc gcttccgcca tgggacgaag
     4801 ctcctgcgat ttcgcgctga caaggcaccc tccgactgca cgtggcgaga gctgaggccg
     4861 ccgcgctccc cggacgaccc gacgttcgag agcctcctgg ggaccgcttg agcgcgctca
     4921 gcggagcgcg ccgacgatga ccgcaccggc gacgacgagc agggcggcga gggcgaccct
     4981 ggctccgatg cgctcgctcg tccggaaggc gaggtgcgcg aaggcgacgg cgaacagtgc
     5041 ctgcgtcgcg ttcagcggag ccacgacggt cacgcgtccc tctgtgaagg caaccacgag
     5101 cgtgccgagc gcccctgcga acacgagtgc cgcagggagc atcggcttga gggaggcacg
     5161 cacgcgcacg agcaactcgc gcctgctcgc cgaggcgaga atcagccagc ctgcgacggc
     5221 gcccgcggcg agcggagctg cacccgcctg gagcgaagga acgtcgacgt ggtcggcggc
     5281 cagccgcact gcgttgtccc ggaacgcgaa cagcagcgcg cagactgccg ccaggacgag
     5341 tccgatgcgt cggatgtgct cgggccggag tctttccctc gccagcagtg tcgtgccgcc
     5401 gacgatgaac gctgtgccga cgaggaccgg ccacgtcagc ggctcgccca ggaacaccag
     5461 cgcgatcccg accgagagaa ccggcgcggt gccgatgacg atcgacggac gggcggcgcc
     5521 cgcagcccgc accgagcgct ggtagaggaa ctgcgtcgtc cccggaacgg cgagcccgat
     5581 gaggacgtac ggccagagct cgccgagggc gagcgagtcg ccggtgacgg cgagaccgac
     5641 cccggcgact ccgaggatcg caaccgacgc gatcgtgatc accgcaccga cacctgcgtc
     5701 caggtcgggc acacgtgcct gcccccagcg gatcgcgacc gagtaggcgc cgaagagaac
     5761 ggccgaaacg agcgagagga cgacggccgc cacgggcgtg caaggctaac gctcggagct
     5821 tctacgtgtc cttgcgcgcc cgggagggtt gcacccgctt gggctcgtcc ggcatcttcg
     5881 ggaagttcgg cggatacggc gcctcgccga gaccttcggt ctcgtcccgc tcgacccatt
     5941 cgagcaggac gcggaggtcg cacacggcct cgtcgatccc agcctgcacg tcccccagct
     6001 cggcgaagcg gccgggcatc gtcgccagcg tgaagtcttc cgtctccacg tcggggagct
     6061 cgtcccagcc cacgggcgcc gacaccgtgg cgtcggcccg gccccgaacg gagtaggcgg
     6121 acgcgatcgt gcggtcgcgg gcgttctggt tgtagtcgat gaagacgcgc ttgccgcgct
     6181 cctccttcca ccacgccgtt gtgaccgccc tcggcgcccg ccgctcgatc tcgcgcgcga
     6241 gcgccagcgc cgcgcggcgc accacgggaa actcccagct cggctcgatc cggcaggcga
     6301 cgtggatgcc acggttccca gacgtcttcg gccagcccgt gtagccgatc tcggcgagcg
     6361 tctcgcgcac gatcccggcc acatgtttcg catcgccgaa gtgcgtcccc ggctgcggat
     6421 cgatgtcgat ccgcagctcg tcgggatgct ccgtgtcggt gcgtcgcgag ggccaggggt
     6481 ggaagtcgac gaccgcgagg ttcgccgccc acgccacctg ggcgagctcg gtcacgcaca
     6541 gctcgtcggc gtggcggccg gaggggaagg tgacgcgcgc cgtgcccacc cagtccggcc
     6601 gatgcttcgg cacgcgcttc tggtagatca tctcgccctc gacgccgtcg gggaagcgac
     6661 gcagctgcgt cggccgctcg tagagggcgc ggacgatgcc gtcaccgacc gcgaggtagt
     6721 actcgacgag atcgcgcttg gtctcgctgc gggccggaaa gagcaccttg tccgggttcg
     6781 tcaccttgac gacgcgctca cccacctcga gctcgatgaa cggcgaatcg gccatgtccg
     6841 ccacgctaca catccccgtg gctcgttcac ttccaaaagt gaagaaatgg acgaagatgt
     6901 ggtagcctcg cggccccgcc acccgcacgt cccaggaggg gtcattccat gcgcaaagct
     6961 ctcgccattt ccctcgcggt cgccgcagcg gccgcgctga cggtcgccgc gagtgcgacc
     7021 ggcggcggct cggcggccgc cgcgaccggc gccgtccccg gctgcgcggt ctcgggcctg
     7081 aacctcctca aggagggcga gctcacgatc ggcgccgaca acccggcgtt cccgccgtgg
     7141 ttcgggggcg ccgagaagga tccgtggaag gtctcgaatc ccttcagccg caaggggtac
     7201 gagtccgcgg tcgcctatgc gatcgcccga gagctcggct tccgcaacag cgccgtcgac
     7261 tggacgttcg tcccgttctc gaagtcgttc cggcccggca agaagccgtt cgacttctac
     7321 gtcacgcagg tctcgatcac gaaggagcgg gccaaggtcg tcgacttctc gaagccctac
     7381 tacttcgtca accaggcgat cgtgggccgc aagggcacgc agatcgccaa ggcgaagtcg
     7441 atcaaggacc tgaagccgta ccgcctcggc gcccagctcg gcacgacgag ctacgccttc
     7501 atcacgagga acatcaagcc gtcgcagacg ccgcgggcgt tcgacacgaa cgatctcgcg
     7561 gtgcaggcgc tgaagaacgg acagatcgac ggcatcgtcg tcgacctgcc gacctccttc
     7621 tacgtgacgg ccgttcaggt cgaggacggc aagatcgtcg gccagttcca gaacaagggc
     7681 ggcaaggagg gcttcggcct ggtgttcgag aagggcaaca agctccgtgc ctgcgtcaac
     7741 aaggcgatcg accggctctg gaagaagggc acgatcgcga agctgcagca ggcgtggctc
     7801 tcgcgggtcg ccggcgcgcc ggtcgtgaag tagcgcactg accggcaacc gcggctcccg
     7861 gatcctcggc gtctcgaccg cccgctcctc ccaggggcgg gcggtcgcta tcgccgtcct
     7921 cagcacgatc gccttcctcg tggcgatcgt cctgctcgtc acgaactcgc ccggctggga
     7981 ggagttcaag gcctacttct tcgactggga caagtacaag gagtccttcc cggagatcgc
     8041 ggaggccttt ctcctcaaca tccggatctt cctgatcgcc gaggcgatca tcctcgtgac
     8101 ggcgctgatc ctggcggtgc tgcgtgggct tcccgggccc gtcctcttcc ccgtccgcgc
     8161 cttcgctgtc gtctacgccg acttcttccg cggcgtgccg acgatcctcg tgatcctcat
     8221 gctgggaccg ggactgccgg cgctcggcgt cgagggaatc ccgaccgatc cggtgttctg
     8281 ggcggtcgtg tcgctcgtcc tcgtctacac cgcctatgtc tcggaggtct atcgagcagg
     8341 catcgagtcc gtgcacccga gtcagagcgc ggcggcgcgc tcgctgggac tgagccacat
     8401 gcagtcgatg cgatacgtga tcatccccca ggcggtgcgc cgtgtgatcc cgcccttgct
     8461 caacgacttc atcggcctgc agaaggacac cgcgctcgta agcgtgctgg gcgtcgtcga
     8521 ggtgctgcgg cagacgcaga tcaacgaggc ggcgacgttc aacggcacct actacctggt
     8581 cgcggcgacg ctcttcgtcg cgctgacgat cccactcgcc cgcttcaccg actggctcgt
     8641 cgcgcgtgac cgcaaacgcc gccaggcggc ggcaacgaca tgaaggcccc tgccgaggcg
     8701 ctgcgggtcg agggcgtgtg gaagtccttc gacaagctcg aagtgctgcg cggcatcgat
     8761 ctcgtcctgg ccgagcacga ggtcgtctgc atgatcgggg cctcagggtc gggcaagtcg
     8821 acgctgctcc gctgcgtcaa ccttctcgag acgatcgacg acggacgaat cgtcctccac
     8881 ggcgaggaga tcaccggcca gggcgtgaac gtcgacgcaa tccgtcgcgg gatcgggatc
     8941 gtcttccagg ccttcaacct cttcccccac atgtcggtgc tgcgcaacgt gacgctcgct
     9001 ccgcgaaagg cgctgggtct gtcgaagaaa gcggccgagg
//
LOCUS       DBNGNK010000064         3110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_727318,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000064 DBNGNK010000000
VERSION     DBNGNK010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_727318"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="AC150V_01640"
     CDS             complement(<1..478)
                     /locus_tag="AC150V_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmdB family metallophosphoesterase"
                     /protein_id="HIR3761656.1"
                     /translation="MRILFIGDVVGRPGRDAIEDRLPLLKSDLEIDFCILNGENLADG
                     VGITPKLAERMLSAGADAITLGNHTWRRQEIAPYLAESERVIRPANLSAAAPGLGMTV
                     LPARNGSSVAVVNVLGSLYLEPARSMWEVVDELVSTARRSAKVIVVDIHAEATSEKI"
     gene            complement(485..1243)
                     /locus_tag="AC150V_01645"
     CDS             complement(485..1243)
                     /locus_tag="AC150V_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761657.1"
                     /translation="MATARSRQRARVDARRRRRRQRTRRLAVLAFLGALGLITLLLTA
                     FGSSSPSRDAVVDQPTPIPAGLRPKAEIVATTSNLRIQLPVAQGAVTAIGFHGARDGS
                     RELTPLGTQVNEGFLARLWGRLTDNDKSGPDWYQLGGTAGTGTNVLDVGAEAGTDVFA
                     PVDGTVAAISDQVRSGKVAGARVDIRPTAAPSLMVSVTNLLADPSLDVGATVAAASSK
                     LGTVADVAELERQAIADVSHDEGNNVSIEVFTAP"
     gene            1317..2273
                     /gene="hemB"
                     /locus_tag="AC150V_01650"
     CDS             1317..2273
                     /gene="hemB"
                     /locus_tag="AC150V_01650"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR3761658.1"
                     /translation="MSTFPATRLRRLRRTEALRALARETRLELDDFVMPLFACPGEGV
                     SRPVAGLPGIAQQSIDVLVDEARELVELGVRAVLLFGVPEEKDDVGSGAWDDDGIVQR
                     TLHALRREAPGLMLVTDVCLCEYTSHGPCGVLVDGEVANDETVELLARTAASHVEAGA
                     DVVAPSDMMDGRVAAIRARLPQTPIIAYAAKYASAFYGPFREVAESTPAFGDRRGYQM
                     DVANVREALRECELDLREGADVLMVKPALPYLDVIRAARDRFDCPLAAYNVSGEYAMV
                     KAAAAAGHLDERSAALESLTAIRRAGADLIVSYWTKELAQWL"
     gene            2264..>3110
                     /locus_tag="AC150V_01655"
     CDS             2264..>3110
                     /locus_tag="AC150V_01655"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR3761659.1"
                     /translation="MALTETSALWLRARQLIPGGVNSPVRAMRSVGLEEPLFMRRGAG
                     ARIEDVNGRSYVDWVMSWGPLIFGHADPETLDEIERAARQGTTFGAPTEAEVELAAEI
                     VDAVPSVEQVRLVSSGTEAAMSVLRLARAVTSRDRVIKFAGCYHGHVDALLASAGSGL
                     ATLGIPSTPGVPSAVAADTVVVPFNDVDAAAAAVERYGEGVAAVLVEPVAGNMGVVPP
                     EPGFLEALRTLCDANGSLLVFDEVITGFRVARGGAQELYGVEPDLTILGKIVGGGLPA
                     AAFGGR"
BASE COUNT          438 a         1051 c         1134 g          487 t
ORIGIN      
        1 cgatcttctc gctcgtcgct tccgcgtgga tgtccaccac gatgaccttg gccgagcggc
       61 gggccgtgct cacgagctcg tccaccacct cccacatgct ccgagccggc tccaggtaga
      121 gggagccgag gacgttgacg accgcgaccg agctcccgtt gcgagcaggg aggacggtca
      181 tccccaagcc gggagccgcg gccgacaggt tggccgggcg aatcacacgc tctgactcgg
      241 cgaggtacgg agcgatctcc tgccgccgcc aggtgtggtt gccgagcgtg atggcgtcgg
      301 cccccgcgga cagcatccgc tcggccagct tcggggtgat ccccacgccg tcggcgaggt
      361 tctccccgtt cagaatgcag aagtcgatct cgaggtcgga cttgagcagc ggaaggcgat
      421 cctcgatcgc atcacgccca ggacgcccga cgacgtcgcc gatgaagagg atccgcacgg
      481 cggttcaggg ggccgtaaag acctcgatcg agacgttgtt gccctcgtcg tgtgacacat
      541 ccgcgatcgc ctggcgctcg agctcggcga cgtcggcgac ggtcccgagc ttggagctcg
      601 cggccgcgac ggtcgctccg acgtccaatg agggatccgc caggaggttg gtcacggaca
      661 ccatcaggga cggcgcggcc gtcggccgga tgtcgacgcg tgctccggcc accttcccgc
      721 tcctgacctg gtcgctgatc gcggcgaccg tgccgtcgac cggcgcgaac acgtcggtac
      781 cggcctccgc cccgacgtcg agcacattcg tcccagtgcc ggcggttccg ccgagctggt
      841 accagtccgg gccggatttg tcgttgtcgg tcagacgccc ccagagtcgg gcgaggaagc
      901 cctcgttgac ctgtgttccg agcggcgtga gctcccggga gccgtcgcgc gcgccgtgga
      961 agccgatcgc ggtcaccgcg ccctgagcga cgggaagctg tatacggaga ttggacgtcg
     1021 tcgcgacgat ctcggctttg ggccgcaggc cggcagggat gggcgtcggc tggtcgacga
     1081 cggcgtcgcg ggagggtgag ctcgacccga atgctgtcag caggagcgtg atgaggccaa
     1141 gcgctcccag gaaggcgagc acggcgaggc gccgtgtccg ttgccgcctg cgacggcggc
     1201 gtgcgtcgac acgggcccgc tggcgggagc gcgccgtggc catcgacgga ccgtagctga
     1261 ggttcgggac accctcaaca gtctgcgctc gcgccggctg acgtacgctt tccccggtga
     1321 gcacgttccc cgccacccgg ttgcggcggc tgcgccgaac ggaggcgttg cgcgcgctcg
     1381 cgcgtgagac gcggctcgag ctcgacgact tcgtgatgcc gctcttcgcc tgcccgggcg
     1441 agggagtgag ccggcccgta gccggacttc ccggcatcgc gcaacaatcg atcgacgtcc
     1501 tggtcgacga ggcacgcgag ctcgtcgagc tgggcgtccg agcggttctc ctcttcgggg
     1561 ttcccgagga gaaggacgac gtcggctccg gagcatggga cgacgacggg atcgtgcagc
     1621 gcaccctgca tgcgcttcga cgggaggcgc ccggcctgat gctcgtcacc gacgtctgtc
     1681 tctgcgagta cacgtcgcac ggcccttgcg gcgtcctggt cgacggcgag gtggcgaacg
     1741 acgagacggt cgagctgctc gcccgcaccg cggccagcca cgtcgaggcc ggagcggacg
     1801 tggtggcacc gagcgacatg atggacggtc gcgtcgctgc aatccgcgca cgtttgcctc
     1861 agacgccgat catcgcctat gcggccaagt acgcgtctgc cttctacggg ccgttccgcg
     1921 aggtggccga gtcgacgccc gccttcggcg accggcgtgg ctaccagatg gacgtggcga
     1981 acgttcgcga ggcactgagg gagtgcgagc tcgacctgcg ggaaggggcg gacgtgctga
     2041 tggtcaagcc ggcgttgccg tatctggatg tgattcgcgc cgcccgtgac cgcttcgact
     2101 gcccgctggc cgcctacaac gtctccggcg agtacgcgat ggtgaaggca gccgccgctg
     2161 ccggccacct cgacgagcga tcagcggcgc tcgagtcgtt gacggcgatc cggagagccg
     2221 gcgcagatct gatcgtctcg tactggacga aggagctggc ccaatggctc tgaccgagac
     2281 ctcggccctg tggctgcgcg cccggcagct catccccggg ggcgtgaact cgcccgtccg
     2341 cgcgatgcgc tcggtcggac tggaggagcc cctcttcatg cggcgcgggg cgggcgcgag
     2401 gatcgaggac gtcaacgggc ggagctatgt cgactgggtc atgtcgtggg ggccgttgat
     2461 cttcgggcac gccgatccgg agaccctcga cgagatcgag cgagccgccc ggcaggggac
     2521 gaccttcggt gcgccgaccg aggccgaggt ggagctggcg gcagagatcg tcgacgcggt
     2581 tccgtcggtc gagcaggttc gtcttgtctc ctccggcacg gaggccgcga tgagcgtcct
     2641 ccgcctggcc cgcgcggtga cgagccgcga ccgcgtgatc aagttcgccg gctgttacca
     2701 cgggcatgtc gacgcgctcc ttgccagcgc cggttcgggc ctggcgacgc tgggcatccc
     2761 gtcgacgccc ggcgtcccga gcgccgtggc ggccgatacc gtcgtcgttc cgttcaacga
     2821 cgtcgacgcg gcggccgccg cggtcgagcg ctacggcgag ggagtggccg ccgtcctggt
     2881 cgagccggtc gccggcaaca tgggggtcgt tccccccgag cccggattcc tcgaggcgct
     2941 ccgcacgctc tgtgacgcga acgggtcatt gctcgtgttc gacgaggtga tcaccggctt
     3001 ccgcgtcgcg cgcggcgggg cgcaggagct ctacggggtc gagccggacc tgacgattct
     3061 cgggaagatc gtcggcggcg gcctgccggc ggccgctttc ggcggccgcg
//
LOCUS       DBNGNK010000065         2400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_583792,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000065 DBNGNK010000000
VERSION     DBNGNK010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_583792"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="AC150V_01660"
     CDS             <1..371
                     /locus_tag="AC150V_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761660.1"
                     /translation="LAERDKATPDGKAAPGAPDAELLEQIEQRLAELKEAEQVFLRTQ
                     EELADRSEAVAARERLVSQREKELDEREDGWAQPELAELEARLKRLERQQRDVETTQS
                     FSGGLGRLSREGTRRKPGAP"
     gene            complement(375..2246)
                     /locus_tag="AC150V_01665"
     CDS             complement(375..2246)
                     /locus_tag="AC150V_01665"
                     /inference="COORDINATES: protein motif:HMM:NF021355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HIR3761661.1"
                     /translation="MKRAIVISLGLVAIGAGLIAAGSAEPSPAATPRLESFGSCGQLL
                     GYMRKQARPLVGPYGIGGGVIAVDRAVGAPESQAGAVPAPGAGVDYSTTNVQEEGVDE
                     PDIVKSNGRFLYIAGSNTLRIVDVRARTPRLVSTLKLGGGGGHELLLHADRLLVLTSG
                     GAWIDTLPGGIAEERIVAPDRLGSSLTEIDVSDPSAPKIVRTLALEGNYLSARLVGSS
                     ARVVVTSPLPNPIPFEQPSGPSDGAAAAATKRNRTILGKTGLAAWLPTYELRNARGKM
                     VAKKPIVQCRNVIRPASFSGLGMMTVITLDLLRGLEPVDSDSLVTDGQVVYGSKSRLY
                     VATQRWLDRPIPAGSIAPQKVTTAVHAFDISDPLRTHYRASGSVSGHLLNQWSLSEHQ
                     GVLRVASTEFPTWWQGTQQTESESFVTTLRERGDLLVEAGRVGGLGRGERVYAVRFMG
                     DKGYVVTFRQVDPLYTLDLSDPAAPRVRGELKILGYSAYLHPLPGDLLLGVGQDATEE
                     GRVLGTQLSLFDVSNPQLPKRLHKQTIGGWSEAEWDHHAFLYWAPERLAVLPTQRGAS
                     GFRVGRAGIQQAGVVEHPTRRSVVVRGTLYSVSELGVQAADLRTFAPVGSVPFPPVG"
     gene            2325..2397
                     /locus_tag="AC150V_01670"
     tRNA            2325..2397
                     /locus_tag="AC150V_01670"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2358..2360,aa:Ala,seq:tgc)
BASE COUNT          362 a          809 c          899 g          330 t
ORIGIN      
        1 ccctggccga gcgcgacaag gccacgcccg acgggaaggc cgctcccggc gcgcccgacg
       61 cggagctgct ggagcagatc gagcagcggc tggcggagct gaaggaggcg gagcaggtct
      121 tcctgcggac gcaggaggag ctggccgacc ggagcgaggc ggtcgccgcc cgcgagcgcc
      181 tggtctccca gcgcgagaag gagctcgacg agcgcgagga cggctgggcg cagcccgagc
      241 tcgccgagct ggaggcgcgg ctgaagcgcc tcgagcgcca gcaacgcgac gtcgagacga
      301 cacagtcctt ctcgggcggc ctcggccggc tcagccgcga gggtacgcgc cgcaagcccg
      361 gcgccccgta gggcctagcc gaccggcggg aagggcacgg agccgacggg cgcgaacgtg
      421 cgcaggtcgg cggcctgcac gccgagctcc gagaccgagt agagcgtgcc gcgcacgaca
      481 accgaccgcc gggtcggatg ctccacgacc ccggcctgct ggatgcccgc ccgcccgaca
      541 cggaagcccg aggcgccacg ttgggtgggc agcacggcga ggcgctcggg cgcccagtag
      601 aggaaggcgt ggtgatccca ctcggcctcc gaccagccgc cgatcgtctg cttgtgcagg
      661 cgcttcggca gttggggatt cgagacgtcg aagagcgaga gctgcgtgcc gagcacgcgg
      721 ccttcctccg tcgcgtcctg gccgacgccg aggagcagat cgcccggcag cggatgcagg
      781 taggcggagt agcccaggat cttcagctca ccgcgcacgc gcggagccgc cgggtcggag
      841 agatcgagcg tgtagagcgg gtcgacctgg cggaaggtga cgacgtaccc cttgtcgccc
      901 atgaagcgga cggcgtagac gcgctcgcct cggcccagac cgccgacgcg cccggcctcg
      961 acgagaaggt cgccgcgctc gcgcagggtc gtcacgaagc tttcgctctc ggtctgctgc
     1021 gttccctgcc accaggtggg gaactccgtg ctcgccacgc gcaggacgcc ctggtgctcc
     1081 gagagcgacc actggttgag caggtggccg ctgaccgagc cgctggcacg gtagtgcgtg
     1141 cggaggggat cgctgatgtc gaaggcgtgt acggcggtgg tgaccttttg cggcgcgatc
     1201 gacccggccg ggatcggccg gtcgagccag cgctgcgtgg ccacgtagag gcgggacttc
     1261 gacccgtaca cgacctggcc gtccgtgacg agggagtccg agtcgacggg ctcgaggccg
     1321 cgcagaaggt cgagcgtgat gaccgtcatc atcccgagac cggagaacga cgccggccgg
     1381 atcacgttgc ggcactgcac gatcggcttc ttcgcgacca tcttgccgcg ggcgttgcgg
     1441 agctcgtagg tcggcagcca ggcggcgaga cccgtcttgc cgaggatggt ccggtttcgc
     1501 ttggtggccg cggcggccgc gccgtcagag gggccgctcg gctgctcgaa cgggatcggg
     1561 tttggcaggg gcgaggtgac gacgactcgt gcgctgctgc cgacgagcct ggcgctcagg
     1621 tagttgcctt cgagggcgag tgtccgcacg atcttcggcg cggacggatc gctgacgtcg
     1681 atctcggtga gcgacgagcc gagccggtcg ggcgcgacga tccgctcctc tgcgatgccg
     1741 ccggggagcg tgtcgatcca cgcgccgccg ctcgtgagga cgagcagacg gtcggcatgg
     1801 agcaggagct cgtggccgcc gccgcctccg agcttgagag ttgagacgag gcgaggcgtt
     1861 cgcgcgcgaa cgtcgacgat ccgcagggtg ttgctgccgg cgatgtagag gaagcgaccg
     1921 ttcgacttca cgatgtcggg ctcgtcgact ccctcctcct gcacgttcgt cgtcgagtag
     1981 tcgacgccgg cgccgggagc gggcaccgcg cccgcctggc tctcgggcgc tcccacggcg
     2041 cggtcgacgg cgatgacgcc gccaccgatt ccgtacggcc cgacgagggg tcgcgcctgc
     2101 ttcctcatgt agccgagcaa ctgcccgcaa gagccgaagc tctccagccg cggcgtcgcc
     2161 gccggtgacg gctcggcgct gcccgcagcg atcagcccgg cgccgatcgc gacgagtccc
     2221 agcgagatga cgatcgcccg tttcatggtt ctcgactggt tatacgccgc cgcccgcgga
     2281 aatgtcaccc gcgcccgggg tagtccctag aatcgatggc gaacggggcg ttagctcagc
     2341 tgggagagcg ccggctttgc aagccggagg tcaccggttc gatcccggta cgctccactc
//
LOCUS       DBNGNK010000066         6148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1229631,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000066 DBNGNK010000000
VERSION     DBNGNK010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1229631"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC150V_01675"
     CDS             <1..233
                     /locus_tag="AC150V_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761662.1"
                     /translation="SVRATAISITSQADAVGQWTGGPALGAVGNVFSVRAALAAGALC
                     LLPALGLYRRAIRHQSLQSKDLESAIEEPRQA"
     gene            299..751
                     /locus_tag="AC150V_01680"
     CDS             299..751
                     /locus_tag="AC150V_01680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3761663.1"
                     /translation="MKHFFTNHDLDTEGCGQFVDITDDVLEAVSESDVQNGTVTVYSP
                     HTTCAVVINEPESGFVDDFRRLLDELVPQEGRYYRHDDMDVRTECIDPGGDLPNGHSH
                     CRAALLASASQSIPVVGGKLMLGRWQKVFFCELDRARSRKVFVQVVGE"
     gene            748..1632
                     /gene="hpnC"
                     /locus_tag="AC150V_01685"
     CDS             748..1632
                     /gene="hpnC"
                     /locus_tag="AC150V_01685"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - geranylgeranyl diphosphate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HIR3761664.1"
                     /translation="MSGTASVTSPLSRPPLTGLAAEAVLAKASRENFPVALRVLPRET
                     RERLQAVYGFARLVDDIGDCAPGDRLALLDWVEADLERAYNGRAEHPLMQRLTPLVAA
                     CEVPRDPFLRLIEANRRDQRQGRYQTFEDLCEYCTFSATPVGELVLHVFGVCTPERLR
                     LSDAVCTALQLVEHWQDVGEDYARGRIYVPLEDLERFGVSEAELAGDRPSESFRRLMA
                     FEVGRARPLLASGIPLVASLRGHARLAVAGYVAGGKAALDAVERSGYDVLARSPSAGN
                     TRRLAAAVAVLGKAGMAR"
     gene            1629..2462
                     /gene="hpnD"
                     /locus_tag="AC150V_01690"
     CDS             1629..2462
                     /gene="hpnD"
                     /locus_tag="AC150V_01690"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046905 - 15-cis-phytoene synthase
                     activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HIR3761665.1"
                     /translation="MNVADAYASCEELTRRSGTSFQLGIRLLPPERRNGMYAIYAFAR
                     RVDDIADGELDPSSRLTELAQARRDVQELTARPDDPVLVALADAHDRFGLPLEAFSDL
                     VDGADMDVTGETYETFDELVVYCRRVAGTIGRLSAAVFGAAEPERSQLLADDLGVAFQ
                     LTNILRDVREDREQGRTYLPREDIDRFGAELDCPTPAFVELIRFEAARADAWYERGLA
                     LLPLLDRSSATSVSAMAGVYRRLLRRVARDPERVLGERLSLPTWEKGWVAARSLVGAR
                     A"
     gene            2423..3802
                     /gene="hpnE"
                     /locus_tag="AC150V_01695"
     CDS             2423..3802
                     /gene="hpnE"
                     /locus_tag="AC150V_01695"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HIR3761666.1"
                     /translation="MGGCAQPRGSAGVTRVTVVGGGLAGITAALMCADRGASVTLLEA
                     RAWLGGATFSVEREGLWLDNGQHVFLRCCPAYRSLLRRLGVEHLVHLQERLDVPVLSP
                     GVGIGRLRRGSLPAPLQLFGSLALYPFLTPLDKARLSRAVVGLQRLQLGDAELDRRTF
                     GDWLRDRGQSPTAIEGLWDLITLPTVNLATDEASLALAAKVFKTGLLEDARSGDIGYA
                     TVPLQRLHGDAAADALAAAGVTVRLRAPATAILPEPEAVTVAWKGGSVSSDAVVVAVP
                     HDLLDGLLPAATLSDSVVPVLLGASPILNLHVLYDRPVTRLPFAAALQSPVQWVFDRT
                     GSSGLSDGQLLAVSLSAADAFVDRPLAELRSVFVPALEQLFPAARGATVRSFFATREP
                     RATFRGVPGTAQHRAGTATRESRLFLAGAWTDTGWPATMEGAVRSGLAAAERALSLER
                     NRPDRELAA"
     gene            3799..5679
                     /gene="shc"
                     /locus_tag="AC150V_01700"
     CDS             3799..5679
                     /gene="shc"
                     /locus_tag="AC150V_01700"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HIR3761667.1"
                     /translation="MTSVVDRPGATAAEAALERGVDRLLALQASEGWWKGELETNVTM
                     EAEDLLVREFLGIRTDEETRLTANWIRSKQRADGSWAVFHGGPPEPSTTIEAYTALRL
                     AGDPADARHMRLAAEYVRDAGGLEHARVFTKIWLSLFDLWSWRDVPAMPPELVFLPPS
                     FPLNLYDFACWARQTVAALTVAWAYRPVRPFPVRVDELRTGGPPGTRRPVSDGWGRAF
                     LQLNRVLHAYERRPIAPVRRRALDRVERWIVERQEADGSWGGIQPPWVWSLIALSLRG
                     YPLDHPVMQLGLEGLDGFTVVEGDVRRLEACQSPVWDTALAMIGLADAGLPADHPAQA
                     KAAEWLLAEEITHRGDWTVRRPKLSAGGWAFEFENDWYPDIDDTAIVVLALRRVETDR
                     EIDAAARRGVAWTLGMRSASGAWGAFDADNCRALVRRLPFCDFGEVIDPPSADVTAHV
                     VEMLAREGRAREPVTVRAVDWLLSEQEPDGSWFGRWGANYVYGTGAVVPALVAAGLEL
                     DHPSIRRAVAWLERVQNADGGWGEDLRSYTDPAWSGRGHSTPSQTAWALLALDAAGEA
                     GAALEQGVRWLVENQLQDGSWDEPYFTGTGFPGDFYINYHLYRLVFPVSALGRIVRRR
                     VG"
     gene            5727..>6148
                     /locus_tag="AC150V_01705"
     CDS             5727..>6148
                     /locus_tag="AC150V_01705"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019284 - obsolete L-methionine salvage
                     from S-adenosylmethionine [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA];
                     GO_process: GO:0046124 - purine deoxyribonucleoside
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase family protein"
                     /protein_id="HIR3761668.1"
                     /translation="MRGAVSGARVLRTGMGRESSIAAADALRGSDAAALAVVGVCGGA
                     REGLAPGDVVVATEVRDGNGSVRPLPGSSLLAGALRRAGHRVTTGPVASTEALAGPAE
                     RAALAHQGVVAVDMESAWLASAAAGKPLAVLRVVADAEG"
BASE COUNT          821 a         2150 c         2161 g         1016 t
ORIGIN      
        1 cgagcgtccg cgccacagcc atctccatca ccagccaggc cgatgcggtt ggccaatgga
       61 ccggcggccc ggctctcggt gcggtcggga acgtcttttc cgtgcgcgca gcgctggcgg
      121 ccggggcgct ctgccttttg cccgccctag gcctgtaccg acgggcgatt cggcaccagt
      181 cgttgcagtc gaaagacctg gaatctgcga tcgaagaacc ccgacaggcc tagccaagac
      241 gaggcgagtc tgatatgtca cgattcaaat cgtcccgacc tacgcttcta ccgcctagat
      301 gaagcacttc ttcacaaatc acgatttgga caccgagggc tgtggccagt tcgtggacat
      361 caccgacgac gtcctcgaag cggtgtccga gagtgacgtc cagaacggca ccgtcaccgt
      421 ctactccccc cacacaacct gcgccgtcgt catcaacgag cccgagagcg ggttcgtcga
      481 cgacttccgc cgccttctcg acgagctcgt accgcaagag gggcgctact accgccacga
      541 cgacatggac gtccgcacgg agtgcatcga tcccggcggc gatctgccga acggtcactc
      601 gcactgccgg gcggctctgc tggcctcggc ctcgcagtcc attccggtcg tcggcgggaa
      661 gctgatgctc ggtcgctggc agaaggtctt cttctgcgag ctcgaccggg cacggtcgcg
      721 gaaggtgttc gtccaggtcg tgggggagtg agcggaacgg cttccgtcac atcgccactc
      781 tctcggccgc cgctcaccgg cctggctgcg gaggcggtgc tcgcgaaggc gtcacgggag
      841 aacttccccg tcgcgctccg cgtgcttccg cgcgagacga gagagcgcct ccaagccgtg
      901 tacggcttcg ctcggctcgt cgacgacatc ggcgattgcg cgccgggtga ccggcttgcg
      961 ctcctcgact gggtcgaggc cgacctcgag cgcgcctaca acggccgcgc cgagcaccct
     1021 ctgatgcagc gtctcacgcc gctcgtagcg gcgtgcgaag tgcctcgtga ccccttcctg
     1081 cggctgatcg aggccaatcg gcgcgaccag aggcagggtc gctaccagac gttcgaggac
     1141 ctgtgcgagt actgcacctt ctcagccacg ccggtgggcg aactcgttct ccacgtcttc
     1201 ggcgtttgta cgccggagcg gctccgtctg tcggatgccg tctgcactgc cctgcagctg
     1261 gtcgagcact ggcaagacgt cggcgaggac tatgcacgag gcaggatcta cgttccgctc
     1321 gaagatctcg agcgcttcgg cgtgagcgag gcggagctcg cgggcgatcg cccgagcgag
     1381 tcgtttcgcc ggctcatggc gttcgaggtc ggacgggctc gtccgttgct cgcgtcgggc
     1441 atcccactcg tcgcgtcact gcgaggccac gcgcggttgg cagtcgccgg ctacgtcgcg
     1501 ggtgggaagg ccgctctcga cgccgtcgag cgatccggct acgacgtgtt ggcgcggagt
     1561 cccagcgccg gcaacacaag gcgtctggcc gccgcagtgg ccgtgctcgg caaggcgggg
     1621 atggcgcgat gaacgtcgcc gacgcctacg catcctgcga ggagctcacc cgccggagcg
     1681 ggacgagctt ccagctcggg atccgcttgc tgccgcccga gcggcgcaac ggcatgtatg
     1741 cgatctacgc cttcgcccgc cgtgtcgacg acatcgccga cggcgagctc gacccctcgt
     1801 cccgcctgac cgagcttgcg caggcgcgtc gggacgtgca ggagctgacc gcccgtcccg
     1861 acgatcccgt cctcgtggcg ctcgcggatg ctcacgaccg gttcgggcta ccgctggagg
     1921 cattctcgga cctcgtcgac ggagccgata tggacgtcac gggcgagacg tacgagacgt
     1981 tcgacgagct cgtcgtctac tgccggcggg tcgccggcac catcgggagg ctctcggccg
     2041 cggttttcgg cgcggcggag cccgagcggt cgcagctgct cgcggacgac ctcggcgtcg
     2101 ccttccagct gacgaacatc ctgcgtgacg ttcgcgagga ccgtgagcag ggacggacgt
     2161 acctgccgcg ggaggacatc gatcgcttcg gtgccgagct cgactgccca acgcccgcat
     2221 tcgtcgagct gatccgcttc gaggccgccc gcgctgatgc ctggtacgag cgggggttgg
     2281 cgctccttcc gttgctcgat cgatcgagcg cgacgtccgt ttcggccatg gccggcgttt
     2341 accggcggct tctccggcga gtcgcgcgcg atcccgagcg cgtcctcggc gagcgcttgt
     2401 cgctgccgac gtgggagaag ggatgggtgg ctgcgcgcag cctcgtggga gcgcgggcgt
     2461 gacccgggtc acggtcgtcg gcggcggcct cgccggtatc acagcggcac tcatgtgtgc
     2521 cgatcggggc gcgtcggtca cgctcctcga ggcgcgtgcg tggctcggcg gcgcgacctt
     2581 ctcggtcgag cgcgaagggc tgtggctcga caacggccag cacgtcttcc tccgctgctg
     2641 ccccgcctac cggagcctct tgcgtcgtct gggggtcgaa cacctggtgc acctgcaaga
     2701 gcgcctcgac gtcccggtgc tcagtccggg agtcggcatc ggtcggctgc gccgcggctc
     2761 gcttcctgct ccgctccagc ttttcggctc gctggctctc tacccgttcc tcacgccgct
     2821 cgacaaggct cggctctcgc gcgcggtcgt cggcttgcag cggcttcagc tcggcgatgc
     2881 ggagctcgat cgtcggacgt ttggagactg gctgcgcgac cgcggccagt cgccgacggc
     2941 aatcgagggc ctctgggacc tgatcacgct gcccaccgtc aacctcgcga cggacgaggc
     3001 ttcgctcgct ctggccgcca aggtcttcaa gaccggtttg ctcgaggacg cccgttcggg
     3061 cgacatcggc tatgcgacgg tcccgctcca gcgcctccac ggtgacgcgg ccgcggacgc
     3121 gctcgccgca gccggcgtca cggtccgtct ccgggcaccc gccaccgcga tcctgccgga
     3181 gccagaggcg gtcaccgtgg cctggaaggg aggctcggtc tcgtctgacg ccgtggtcgt
     3241 cgcggtgccc cacgacctgc tcgacggcct gctgcctgca gccacgctct ccgactccgt
     3301 cgttcccgtc ctcctcggcg cttcgccgat actcaacctc catgtcctgt acgaccggcc
     3361 cgtgacgagg ctgccgttcg cagccgccct gcaatcgccc gtacagtggg tcttcgaccg
     3421 gacgggctcc tccgggctga gcgacgggca gctgctggcg gtctcgcttt ccgctgcaga
     3481 cgccttcgtc gatcggccgc tcgccgagtt gcgctccgtc ttcgtcccgg cgctcgaaca
     3541 gctctttccg gcggcgcggg gggccacggt gcggtcgttc ttcgccactc gcgagccgcg
     3601 ggcgacgttt cggggcgtac ccggcaccgc tcagcaccgc gccggaacgg ccacgcgcga
     3661 gtcgcgcctg ttcctggccg gcgcgtggac cgacaccggc tggcccgcga cgatggaggg
     3721 cgccgtccgc agcgggctgg ctgcggcgga gcgcgctttg tctctcgagc ggaatcgccc
     3781 cgacagggag ctggcggcat gacgagcgtc gtcgacagac cgggtgccac tgcggcggag
     3841 gccgcgctgg agcgcggagt cgaccggcta ctcgcactgc aggcgtccga aggctggtgg
     3901 aagggcgagc tcgagacgaa cgtgacgatg gaggccgagg atctcctcgt tcgcgagttc
     3961 ctcgggatcc ggacggacga ggagacgcgg ctgaccgcga actggatccg ctcgaagcag
     4021 cgcgcggacg gaagctgggc cgtctttcac ggcggcccgc ccgagccctc gacgacgatc
     4081 gaggcctaca cggcgcttcg cctcgcggga gacccggccg atgcacggca catgcgtctg
     4141 gcggcggagt acgtgcgtga cgcgggcggc ctcgagcacg cgcgcgtctt cacgaagatc
     4201 tggctctcgc tcttcgatct ctggtcctgg cgcgacgtgc ccgcgatgcc gcccgagctc
     4261 gtcttccttc ctccgtcctt tccgctcaac ctctacgact tcgcctgctg ggcgcgacag
     4321 accgtcgccg ccttgacggt cgcgtgggcg taccgccccg tccgcccgtt cccggtgcgg
     4381 gtcgacgagc tgcggacggg gggcccgcct ggcacccgcc ggccggtgag cgacggatgg
     4441 ggaagggcct ttctacagct gaaccgcgtt ctacacgcct atgagcggcg tccgatcgcc
     4501 ccggtgcgtc ggcgcgcgct cgaccgagtc gagcggtgga tcgtcgagcg gcaggaagcg
     4561 gatgggagct ggggcgggat tcagccgccg tgggtctggt cgctgatcgc gctctccctg
     4621 cgcgggtacc cgctcgacca tccggtgatg cagctcggac tcgagggcct cgacggcttc
     4681 accgtcgtcg agggcgatgt ccggcgcctc gaggcctgcc aatcgcccgt ttgggacacg
     4741 gcgctcgcca tgatcgggct cgccgacgcc ggactcccgg ccgatcaccc ggcccaggcg
     4801 aaggcagccg agtggctcct ggccgaggag atcacccatc gtggcgactg gactgtccgc
     4861 aggcccaagc tgagtgcggg tggctgggcc ttcgagttcg agaacgactg gtaccccgac
     4921 atcgacgaca cggcgatcgt ggtgctggcc cttcggcgcg tcgagacgga ccgggagatc
     4981 gacgcagcgg cgcggcgcgg cgttgcctgg acgctcggca tgcgctctgc cagcggtgcc
     5041 tggggcgcct tcgacgccga caattgccgc gcgctcgtca ggcgccttcc gttctgtgac
     5101 ttcggcgaag tgatcgatcc gccgagcgcg gacgtgacag cacacgtggt cgagatgctg
     5161 gcgagggaag gcagggcacg tgaaccggtc accgtccgtg ctgtcgactg gctgctgagc
     5221 gagcaggagc ccgatggctc ctggttcgga cgctggggag ccaactacgt atacggaacc
     5281 ggtgccgtcg ttcccgcact ggtcgcggcc ggcctggagc tcgaccaccc gtcgatcagg
     5341 cgcgccgtgg cgtggctcga acgcgtgcag aacgccgacg gcggctgggg tgaggacctt
     5401 cgttcctaca ccgaccccgc ctggagcggc cgcgggcact cgacgccctc gcagaccgcc
     5461 tgggcgctgc tcgccctcga cgccgccggt gaggcgggag ccgcgctcga gcaaggggtc
     5521 cgctggctgg tcgagaacca attgcaggac ggcagctggg acgagccgta tttcaccggg
     5581 acaggctttc ccggcgactt ctacatcaac taccacctct accgtttggt cttccccgtg
     5641 agcgcactcg gacggatcgt gaggaggaga gtcggctgag ctcgcacctg ctcctgctcg
     5701 caccgttgcg tgtcgaggcg ctcgccgtgc gcggcgcggt cagcggcgct cgcgtgttgc
     5761 gcaccgggat ggggcgcgag agctcgatcg ccgcggccga cgctctccgc ggctccgatg
     5821 ccgcggcgct cgcggtggtc ggcgtctgtg gcggcgccag ggagggtctc gcccctggcg
     5881 acgtcgtcgt ggcgacggag gtgcgggacg gcaacggctc ggtccggcca ctcccggggt
     5941 cttcgctgct cgccggcgca ctccggcgtg caggtcaccg cgtcacgacg ggaccggtgg
     6001 ccagcaccga ggcgctggcc ggccccgccg agcgcgctgc tctcgcgcac cagggcgtgg
     6061 ttgcggtcga catggagtcg gcctggctgg cttccgccgc cgcggggaag cctctcgcgg
     6121 tgctgagagt ggtcgccgac gcggaggg
//
LOCUS       DBNGNK010000067         4554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1515948,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000067 DBNGNK010000000
VERSION     DBNGNK010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4554
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1515948"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC150V_01710"
     CDS             complement(<1..341)
                     /locus_tag="AC150V_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR3761669.1"
                     /translation="MRVHLERELKLSPGPGFQLFELGGEPLPQRTFLSTYHDTEDLRL
                     ARRGVTFRHRIEDGTGLWQLKLPHGAARIELEEIGAPARPPPPMLELLPAFLRGAELA
                     PVARLRTRRDGV"
     gene            429..1919
                     /locus_tag="AC150V_01715"
     CDS             429..1919
                     /locus_tag="AC150V_01715"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004308 - exo-alpha-sialidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HIR3761670.1"
                     /translation="MRPALIVLLAVLTLTAAAGAASLTGTERADLIVGTRSADTVSAR
                     GGSDRVLTWGDGRRDLVSCGAGLDLVNAERNDAVRPDCETVARQISRDRTTAPRAQRG
                     TQAEPDSAADGDTIVTVFQSGRFADGGAAAIGFSTSEDRGRSWIEGTLPQPKAYSRVS
                     DPVVAYDAARGGVWLAAMLGITTQGGTALLVTRSVDGIRWQPPVVAVGPRRRGASLDK
                     EWISCDGSETSPFRGRCYLAYTDLFLGAIAVAFSDDGGRTWSVPVPAHRFARGAVGAL
                     PVVRPAGDVVVFLTDRDQGPIAVRSTNGGASFQRTGAGGEGAFADLRGVRAPQLVAAD
                     VDPSGKVLAVWSSCALRAACQGNDVLLTESSDGVTWSAPRRLPTRATGSAFLPTVAVD
                     AATGRIGVLFYAATNCSDRCRIDGWLIESADGRSWSRPLRLSARSMELPWIARASGGA
                     MLADYISLSYVRGNPVAVLALAREPLGDRKRQAIFAVTRVAGPAAR"
     gene            complement(1920..2534)
                     /locus_tag="AC150V_01720"
     CDS             complement(1920..2534)
                     /locus_tag="AC150V_01720"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3761671.1"
                     /translation="MANVAIELRPAAPTAVDGLSFARFLDQASDGLFRTMLGSQAGRV
                     VAQAFRLPGHDLSFRHVTFAVREGKSVGMLSAYTGGDHEQASDDPLLRAAGWRVVRLG
                     LVALAARPVLHFIERVPVDDFYVQAVAVDSSCRGLGIGSMLLAHAEERARAHGARRLS
                     LDVDVANEDAVRLYERLGLAVERVSRPARLLGGVRVQRMVRELG"
     gene            complement(2830..3492)
                     /gene="phoU"
                     /locus_tag="AC150V_01725"
     CDS             complement(2830..3492)
                     /gene="phoU"
                     /locus_tag="AC150V_01725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR3761672.1"
                     /translation="MRVELQESLDALEADLQEQGAVVLRAVRGAVQALETQDVELCDE
                     VIAFDDEIDLRFRRIGQAIERLLALQTPVATDLRLVLATLHSSIHLERIGDQCVTIAK
                     LTKLSAQLKQNPQLVEGLRDMGDRVEEMVRIALDAFTDRDLVRARSLVELDELVDRTN
                     RRVVGQVLELARTPETQEWGMRMIVVSRCMERIGDNAVDVGEQIAFVVTGEYHEFGDA
                     SH"
     gene            complement(3489..4364)
                     /gene="pstB"
                     /locus_tag="AC150V_01730"
     CDS             complement(3489..4364)
                     /gene="pstB"
                     /locus_tag="AC150V_01730"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR3761673.1"
                     /translation="MSQTDTPRTDAPRIDSGRIAEVSAGTNADLTSRDKVFSVRDLGV
                     SYSGARALEGVTMDVFKNSVTAFIGPSGCGKSTYIRCFNRMNDLIPGARVEGTVLYHG
                     TDLYERGVDPVEVRRRIGMVFQKPNPFPKSIYDNVAFGPRSLGMKKDMDDRVERCLRQ
                     AAIWDEVKDRLKESALGLSGGQQQRLCIARALATEPDVILMDEPASALDPIATARIED
                     LMHDLKREYTIVVVTHNMQQAARVADMTAFFSVDVHEDGKGRHGVLVEYDATTKIFTT
                     PSDKRTEDYVTGRFG"
BASE COUNT          692 a         1550 c         1571 g          741 t
ORIGIN      
        1 acgccgtctc gtctcgtccg caggcgcgcc acgggcgcga gctcggcccc gcgcaagaac
       61 gcggggagca gctcgagcat cggcggggga ggccgcgccg gcgcgccgat ctcctccagc
      121 tcgatgcgcg cggcaccgtg cggtagcttg agctgccaga ggccggtgcc gtcctcgatg
      181 cggtgccgga acgtcacgcc gcgccgcgca aggcgcagat cttcggtgtc gtggtacgtg
      241 gacagaaacg ttcgctgcgg caacggctcg ccgccgagct cgaagagctg aaagcccggg
      301 ccgggcgaga gcttcagctc gcgctcgagg tggacacgca cgggggaagc gtaatcgggg
      361 tctgcgtccg gtcggcaccg gctcggtgaa gaactggtga gaacctcgcc tgcggctact
      421 ttcactggat gcggcccgca ctcatcgtcc tgctcgcggt tctcacgctg acggcggctg
      481 cgggagctgc atcgctgacc gggaccgaac gcgccgactt gatcgtcggg acgaggtcgg
      541 cggacaccgt gtccgcgcgg ggcggctcgg atcgcgtgct cacctggggc gacgggcggc
      601 gcgatctggt ctcctgcggg gccggactcg acctcgtcaa cgcggagcga aacgacgcgg
      661 tcaggcccga ctgcgagacg gtggcccggc agatctcccg cgatcgcacg accgcgccgc
      721 gtgctcagcg aggcacccag gccgaacccg acagcgcggc ggacggcgac acgatcgtga
      781 ccgtcttcca gagcggccgg ttcgcggacg gaggcgctgc cgcaattggc ttctcgacgt
      841 ccgaagatcg cggtcgctcg tggatcgagg gaaccctgcc gcagccgaag gcgtactcac
      901 gcgtgagcga tccggtcgtc gcgtacgacg ccgcccgagg cggcgtgtgg ctggccgcga
      961 tgctgggtat caccacccag ggcggcacgg cgctgctggt aacccggtcg gtcgacggca
     1021 tccgctggca gccgcccgtg gtcgccgtcg gcccgcgccg gcggggcgcc tcgctggaca
     1081 aggagtggat cagttgcgac ggcagcgaga ccagcccgtt ccgcggacgc tgctatctgg
     1141 cgtacacgga tctgttcctc ggcgccatcg cggtcgcatt ctcggacgac ggcggcagga
     1201 cgtggtcggt acccgttccg gcccaccggt tcgcgcgcgg tgccgtgggt gctctccctg
     1261 tcgtccggcc cgcgggcgac gtggtcgtgt ttctcaccga ccgtgaccag gggccgattg
     1321 ccgtccgctc gacgaacgga ggcgcttcct tccagcgaac cggagccggt ggcgaaggag
     1381 ccttcgccga cctgcgcggc gtccgtgcgc cgcaactcgt ggcggccgac gtcgatccga
     1441 gtggaaaagt gcttgcagtg tggtcgtcgt gcgcgctcag ggccgcttgc cagggcaacg
     1501 atgtcctcct caccgagtcg agcgacggcg tcacgtggag tgcaccgcgc cgccttccga
     1561 cccgggcgac cggaagtgca ttcctgccga ccgtcgccgt cgacgctgcg accggccgca
     1621 tcggcgtcct cttctacgcg gcgacgaact gcagcgatag gtgccgcatc gacgggtggc
     1681 tgatcgagtc ggccgacggg cggtcctggt cgcgtccgct ccgcctctca gctcggtcga
     1741 tggagctccc ctggatcgca cgggcgagcg gcggcgccat gctggccgac tacatctcgc
     1801 tctcctacgt ccggggaaac cccgtcgctg tgctcgctct cgcacgagag ccgctcggcg
     1861 accgcaagcg ccaggcgatc ttcgcggtca ctcgcgtcgc ggggccggcg gcacgatgat
     1921 cagccgagct ccctgaccat ccgctgcacg cgcaccccgc cgagcaagcg ggcaggtcgc
     1981 gagacgcgct cgaccgcgag gccgagccgc tcgtagagtc ggacggcgtc ttcgttggca
     2041 acgtcgacgt cgagggagag ccggcgggcg ccgtgcgctc gggcccgctc ctccgcgtgc
     2101 gcgagcagca tcgagcctat tccaaggccg cggcacgaag aatcgaccgc gacggcctgc
     2161 acgtagaagt cgtccaccgg gacgcgctcg atgaagtgca ggaccggccg cgcggctagc
     2221 gcgaccaggc cgaggcgcac gacccgccag cccgcagctc tcagcagcgg gtcgtcgctc
     2281 gcctgctcgt gatcgccgcc ggtgtaggcg ctgagcatcc ccaccgactt gccctcgcgg
     2341 acggcgaagg tgacgtgccg gaaggagagg tcgtgaccgg gcagccggaa ggcctgggcg
     2401 acgacgcggc ccgcctgtga cccgagcatc gtccggaaga gcccgtccga ggcttgatcc
     2461 aagaagcgcg cgaacgagag cccgtctact gccgtcggcg cggcggggcg gagctcgatg
     2521 gcgacgttgg ccacaccgac agcctggcag agcgggtctc agttgcctct ccgcgccttc
     2581 tgcacgccga ggcggaactg cacctccgac cgcttcagct gcttctggct gagcgcgacg
     2641 tagagcgccc gcttcgatcg cggcctgggt ctgcagcatg tagttgatga aggactgcac
     2701 ctcgggtcgg gcgaacgagg tggccttcac gtagatgaag agcccgcgcg cccgcgcaca
     2761 cggtcgcagc gaccgcgggt cactaaggaa tcgacaaggt gaagaagctg ttccggtcta
     2821 gtgacgcgat caatggctgg cgtcgccgaa ctcgtggtac tcgccggtga ccacgaaggc
     2881 gatctgctcg ccgacgtcga ctgcgttgtc gccgatgcgt tccatgcagc gggagacgac
     2941 gatcatgcgc atcccccact cctgggtttc cggcgtgcgc gccagctcga gaacctgtcc
     3001 gacgacacga cggttggtcc ggtcgacgag ctcgtccagc tccacgagcg agcgcgctcg
     3061 gacgagatcg cggtcggtga aggcgtcaag cgcgattcgc accatctcct cgacgcggtc
     3121 gcccatgtcg cgcagcccct cgacgagttg ggggttctgc ttgagctgcg ccgacagctt
     3181 cgtcagcttg gcgatcgtca cgcactgatc accgattcgc tcgaggtgaa tcgagctgtg
     3241 aagggtggca agcacgagcc gcagatcagt ggcgaccggt gtctgcaacg ccagcaggcg
     3301 ctcgatcgcc tgtccgatcc gccgaaagcg caggtcgatc tcgtcgtcga acgcgatgac
     3361 ctcgtcacag agctcgacgt cctgcgtctc gagtgcctga acggctccac gcaccgcccg
     3421 gagcacgacc gccccctgtt cctgtagatc cgcctcgagc gcgtcgaggc tctcctgcag
     3481 ctcgactctc aaccgaaccg cccggtcacg tagtcctccg tccgcttgtc ggagggcgtg
     3541 gtgaagatct tggtcgtcgc gtcgtactcg acaaggacgc cgtgcctgcc tttcccgtcc
     3601 tcgtggacgt cgacgctgaa gaacgccgtc atgtccgcga cgcgggcggc ctgctgcatg
     3661 ttgtgcgtca cgacgacgat cgtgtactcc ctcttcaggt cgtgcatcag atcctctatg
     3721 cgcgcggtcg cgatcggatc cagtgccgat gccggctcgt ccatgaggat cacatccggc
     3781 tcggtggcca gtgcgcgcgc gatgcagagt cgctgctgct ggcccccgga gaggccgagg
     3841 gcgctctcct tgagccggtc ctttacctcg tcccagatcg ccgcctgccg caggcaacgc
     3901 tccacgcggt cgtccatgtc cttcttcatc ccgagcgaac gggggccgaa ggcgacgttg
     3961 tcgtagatcg acttcgggaa tgggttcggc ttctggaaga ccatgccgat ccggcggcgg
     4021 acctcgactg ggtcgacacc tcgttcatac aggtcggtgc cgtggtacag gaccgttccc
     4081 tccacccgtg ctccggggat gagatcgttc atccggttga agcagcgaat gtaggtcgac
     4141 ttgccgcagc cggacggccc gatgaaggcg gtcaccgagt tcttgaagac gtccatcgtc
     4201 acgccctcga gcgcgcgagc gccgctgtag gaaacgccca ggtcgcgcac gctgaagacc
     4261 ttgtcgcgag aggtgaggtc ggcgttcgtt ccggccgaga cctcggcgat ccgtccactg
     4321 tcgatgcgcg gagcgtccgt tcttggtgtg tccgtctgcg acatgtccct gtcctcgctc
     4381 accagttctg acggtatcgg ttgcgcaaga agatggcgac ggcgttcatg ctgagcacga
     4441 tcacgagcag gacgacagcg ccggctgcgg caagcgtctg aaacgtcggg tcgggctgct
     4501 tcgtccagtt gtagatctgc accggcagcg ccgtgtaggc accgaagacc ttcg
//
LOCUS       DBNGNK010000068         5110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_297639,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000068 DBNGNK010000000
VERSION     DBNGNK010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_297639"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1332
                     /locus_tag="AC150V_01735"
     CDS             160..1332
                     /locus_tag="AC150V_01735"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3761674.1"
                     /translation="MQQQSFRELGASRAVTDALAARSITEPFAIQSLVLPDALAGLDI
                     LAKSPTGSGKTLAFAIPIVERTIEDDRRPSALVLVPTRELAGQVEAELSAIAHKKHLR
                     VAAVYGGMPLRAQAKAGRAAHIVVATPGRLEDLAQRGLLDLRAIKILVLDEADRMLDM
                     GFKPQVDRIVRRLPANRQTMFFSATLDGEVGALARAYTNSPARFEANLPGDTAVGEIE
                     HTFVAVRPETKVDTLIEHIRASEGLTLVFVRTKRGADRLVAKLAGKDVDALAMHGDLS
                     QGQRQRALDRFKTGRVSVLIATDVAARGLDIDDIEHVINYDPPEEDKGYVHRTGRTGR
                     AGRRGSAVTFVLPDQQAETSRVANRLGHREQFEEAGMATARAKLVYTSRRGRRSKW"
     gene            complement(1345..1848)
                     /locus_tag="AC150V_01740"
     CDS             complement(1345..1848)
                     /locus_tag="AC150V_01740"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR3761675.1"
                     /translation="MNLAVGDIVVYGMHGIGRVSACEEQLVVGVTRSVVLIELSDGLR
                     VTLPMGLAGEQLRPLASEADLERIQQALREDRALSGDPWLSRKRSALEKLTGGDPVGL
                     AEIVGDGAQRRRARTAKGSKPNLPSGEKEIYEKAWELLSDEISWARGVQRDEADVWIN
                     EQLAHAS"
     gene            complement(1991..2650)
                     /locus_tag="AC150V_01745"
     CDS             complement(1991..2650)
                     /locus_tag="AC150V_01745"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3761676.1"
                     /translation="MTLTIGDAAPDFQAETSEGPISFHDWIGDSWAILFSHPKDFTPV
                     CTTELGYMAKINPEFERRNVKIIGLSVDPVADHDGWAKDIEETQGQAPNYPIIADADF
                     EVSKQYGMLPTSVAGEAHDRTAADNQTVRNVFVIGPDKKIKLVLVYPMTTGRNFDEVL
                     RVIDSLQLTANHKVSTPVNWQRGDDVIIAGSVSNDEAKELWPDGWEEPKPYIRIVPQP
                     S"
     gene            complement(2771..3598)
                     /locus_tag="AC150V_01750"
     CDS             complement(2771..3598)
                     /locus_tag="AC150V_01750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3761677.1"
                     /translation="MTTTDLLETREAWDVIATGYDEFVTPSHFRLGDQVVQRAGVTQG
                     VRFLDVAAGSGGLSIPAARCGAQVLATDLSPVMLDRLAARAIEEGLMTVETEVMDGHA
                     LALEDDVFDVAGSQFGVMLFPELPRALGEMARVTKPGGRLVLAVFGPPDQVEFLTVFV
                     EALQSAVPGFEGLPADPPPLPFQVADPEKLRRALAAVASGEVRIETVTESLQFTSAAS
                     MWNWVVNSNPIGAMLVADLTADQRAEAEHVLGVMLDERSIGGTAVLANPVHIGVATV"
     gene            complement(3595..4503)
                     /locus_tag="AC150V_01755"
     CDS             complement(3595..4503)
                     /locus_tag="AC150V_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3761678.1"
                     /translation="MSTQLEVQHNQPAAEARSRLLAGIPVVERRLDLAGISTAVIEGG
                     EGRPVVLLHGPGEFAAKWTRALPGLVAEHHVVAPDFPGHGASELGSGGLDAGRVFLWL
                     DELIERTCPTPPVLVGHLLGGAVAARFAVDHGEGLAALVLVDSFGLGRFRPSPTFALA
                     LARFMARPSERSYERFMGYCLADREGVRREMGENWDSLRDYSLDRARSEDVKTAMRFF
                     MRELGVPVIPPADLSRISVPTTLIWGRKDRANRIGIAERAHERYGWPLHVIDGAADDP
                     PLDQPQAFLEALRLATTHTPRTRRES"
     gene            complement(4619..>5110)
                     /locus_tag="AC150V_01760"
     CDS             complement(4619..>5110)
                     /locus_tag="AC150V_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019888097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3761679.1"
                     /translation="SSLLEATADHVRGAVELEAGDTAAALVALRRSAEAWRELDVPYE
                     TARVRVLIGLACRALGDEETAAAEFDASRGVFAELEAAPEVASVDALAHPAPSMRPGG
                     LTKRELEVLTLVAAGKTNRAIAEELVISEKTVARHMSNIFAKLGLSSRAAATAYAYEH
                     DLL"
BASE COUNT          815 a         1809 c         1686 g          800 t
ORIGIN      
        1 cataggccac ccctcgccgc gggcaccggg cgcgagcggg gacgactgct actgtgactg
       61 cacggctggc aatcctgccg gcccgtgaga tgcgcttctc gttcctcctt cggacgttcg
      121 aggtaggcaa gtctcgacgt cagagaagga gtgaactcca tgcaacagca gtctttccgt
      181 gagctcggtg cgtcgcgggc cgtcaccgac gcgctcgccg cccgctccat caccgagccc
      241 ttcgccatcc agtccctcgt cctccccgac gcgctcgccg ggctcgacat cctcgcgaag
      301 tcgccgaccg ggtctggcaa gacgctcgcc ttcgcgatcc cgatcgtcga acgcaccatc
      361 gaagacgacc ggcgcccgag cgctctggta ctcgttccga cgagggagct cgccgggcaa
      421 gtcgaagccg agctcagcgc gatcgcgcat aagaaacatc tccgcgtcgc agccgtctac
      481 ggcgggatgc cgctccgcgc gcaggcgaaa gccggccgcg ccgcgcacat cgtcgtcgca
      541 acgccgggcc gcctcgaaga ccttgcccaa cgtggcctgc tcgaccttcg tgcgatcaag
      601 atcctcgtcc tcgacgaggc ggaccggatg ctcgatatgg gcttcaagcc gcaggtcgac
      661 cggatcgtga ggcggctgcc ggcgaatcgc cagacgatgt tcttctcggc aacgctcgac
      721 ggcgaggtcg gcgccctggc gcgcgcctac acgaacagcc ccgcccggtt cgaggcgaat
      781 ctgcccggcg acaccgccgt aggcgagatc gagcacacgt tcgtggccgt ccggccggag
      841 acaaaggttg acactctcat cgagcacatc cgcgcctcgg agggtctcac cctcgtcttc
      901 gtgcgaacaa agcgcggcgc ggacaggctc gtcgcgaagc tggccggcaa ggacgtcgac
      961 gcgctcgcca tgcacggtga tctctcacag gggcagaggc agcgggcgct cgaccgcttc
     1021 aagacgggcc gcgtgtccgt cctgattgcc acagacgtcg ccgcccgcgg cctggacatc
     1081 gacgacatcg agcacgtcat caactacgac ccgcccgaag aggacaaggg atacgttcac
     1141 cggacgggac ggacgggccg cgccggccgt cgcggatcgg ccgtgacgtt cgtgcttccg
     1201 gaccagcagg ccgagaccag ccgcgtcgcg aatcgcctgg gacaccgaga gcagttcgag
     1261 gaggccggaa tggcgaccgc gcgggcgaaa ctcgtctaca cgagccgccg cggccgacgg
     1321 tcgaagtggt agccggctcg ggcgtcagct cgcgtgggcg agttgctcgt tgatccagac
     1381 gtcagcttcg tcccgctgca cgccgcgcgc ccacgagatc tcgtcggaga gcagttccca
     1441 ggctttctcg tagatctcct tctcgcctga cgggagattc ggcttgcttc cctttgcagt
     1501 ccgcgcccgc ctacgctgag caccgtcccc cacgatctcg gccagcccca cggggtcgcc
     1561 gccggtgagc ttctcgagcg cgctccgctt ccgggacagc cagggatctc cactgagcgc
     1621 gcggtcttct cggagtgctt gctgaattct ctccaggtcg gcctcgctcg ccagtggcct
     1681 gagctgctct ccggccagcc ccatcggcag cgtcaccctg agcccgtccg acagctcgat
     1741 caagaccacc gaacgcgtga cgccgacgac cagttgctcc tcacaagccg acacgcggcc
     1801 gattccgtgc atgccgtaca cgacgatgtc accgaccgca agattcactt actcagccta
     1861 gcgcgcgggt cggccgaggc aacggcacgc gcgggaaagc agggcccgct cgcggctgcg
     1921 cggcgaggcc ggcgccgggc acggcgccgg cctgcacgca cgtagtcgac caagcagccc
     1981 gctgctgcgg tcagctcggc tgcggcacga tccggatgta cggcttcggc tcttcccagc
     2041 cgtcgggcca cagctccttg gcctcgtcgt tcgagacgga gccggcgatg atgacgtcgt
     2101 cgccccgctg ccagttgacc ggcgtcgaga ccttgtggtt cgcggtcagc tgcagcgagt
     2161 cgatcacacg gagcacctcg tcgaagttcc ggcccgtcgt catcggatag acgaggacga
     2221 gcttgatctt cttgtccggg ccgatcacga agacgttgcg aaccgtctgg ttgtcggccg
     2281 ccgtgcggtc atgcgcctcg cccgcgacgc tcgtcggcag catgccgtac tgcttcgaga
     2341 cctcgaagtc ggcgtcagcg atgatcgggt agttcggggc ctggccctgc gtttcttcga
     2401 tgtccttggc ccagccatcg tgatcggcca ccgggtcgac cgacagaccg atgatcttga
     2461 cgttccggcg ctcgaactcc gggttgatct tcgccatgta gccgagctcg gtcgtgcaca
     2521 ccggagtgaa gtccttcggg tgcgagaaca agatcgccca cgagtcgccg atccagtcgt
     2581 ggaagctgat cgggccctct gacgtctctg cctggaagtc cggtgccgcg tcgccgattg
     2641 tcagtgtcat gttccaccct cccgcttgtg aactgaccct cccgttgccc gcggtgcgca
     2701 cttctcgcgc acctcggaca atggacccta caacgggacc cgaatcgctt ttcttccgcc
     2761 tccgggccga ctacacggtc gcgacgccga tgtggaccgg attcgccaga accgcggttc
     2821 cacctattga gcgctcgtcc agcatcacgc ccagcacatg ttccgcctcg gcacgctgat
     2881 cagccgtcaa gtcggcgacg agcatcgcgc cgatcgggtt gctgttcacg acccaattcc
     2941 acatgctcgc cgcggacgtg aactggaggc tctccgttac cgtctcgatc ctgacctcgc
     3001 cggatgcaac ggcggcgagc gctcgccgaa gcttctcggg gtccgcgacc tggaagggca
     3061 gcggaggcgg atccgcgggg agaccctcga agccgggaac ggccgactgc aacgcctcga
     3121 cgaagacggt caggaactcc acctggtcgg gaggcccgaa gacggcgagg acgagccgcc
     3181 caccaggctt tgtcacgcgg gccatttcgc cgagggcgcg cggcagctcg ggaaagagca
     3241 tgaccccgaa ctgggagccg gcgacgtcga agacgtcgtc ctccagcgcg agcgcgtggc
     3301 cgtccatcac ctcggtctcc accgtcatca gcccttcctc gatcgcgcgt gcggcgagcc
     3361 ggtcgagcat cacgggcgag aggtcggtcg cgagcacctg cgcgccgcaa cgcgccgcgg
     3421 ggatgctcag gccgccgctg ccggcggcga catcgaggaa tcgcactccc tgggtcacgc
     3481 ccgcgcgttg caccacctgg tcgccgagcc ggaagtgcga gggcgtgacg aactcgtcgt
     3541 agccggtcgc gatgacgtcc cacgcctcgc gggtctccag caggtcggta gtagtcatga
     3601 ctccctcctc gttctcgggg tgtgggtcgt tgcgagccgc aacgcctcga ggaacgcctg
     3661 cggctgatcc agcggggggt cgtccgcggc gccgtcgatc acgtggagcg gccagccgta
     3721 gcgctcatgg gctctctcgg cgatcccgat ccggttcgcg cggtcctttc gcccccagat
     3781 cagggtcgtg gggacggata ttcgcgagag atccgccggc gggatcaccg gcacgccgag
     3841 ctcgcgcatg aagaagcgca tcgcggtctt tacgtcctcg gaacgggccc ggtcgagcga
     3901 gtagtcgcgc agcgagtccc agttctcgcc catctcgcgg cggactccct cgcggtcggc
     3961 caggcagtag cccatgaagc gctcgtacga gcgctcgctc ggccgggcca tgaagcgcgc
     4021 gagcgccaga gcaaacgtcg gtgaaggccg gaagcggccg agcccgaacg agtccacgag
     4081 aacgagcgcg gcgagtccct cgccgtggtc gacggcgaag cgcgcggcaa cagcacctcc
     4141 gagcagatgc ccgacgagga caggcggggt cggacaggta cgttcgatca gctcgtcgag
     4201 ccacaagaag acgcggccgg cgtcgaggcc gcctgagccg agctcggagg cgccgtgtcc
     4261 tgggaagtcg ggcgccacga cgtggtgttc ggccacgaga cccggaagcg cccgcgtcca
     4321 tttcgccgca aactcgcctg gaccgtgcaa gaggacgacg ggccttcctt cgccgccctc
     4381 gatgaccgcc gtggagatcc cggccaggtc gagacgacgc tcaacgacgg gaatcccggc
     4441 caggagccgc gaccgagctt ccgccgccgg ctgattgtgc tgcacctcga gctgagtgct
     4501 catcgctgct gcctctcgtt ggggtcggct gctcgctcac gatggcgcgt gcgcgggcgc
     4561 acgacatcgg ccgatgtacc caattgccgc gcctcgcgga gtgggtagat ccacccagtc
     4621 agagcaggtc gtgctcgtac gcgtaggccg tcgcggccgc ccgggacgag agcccgagct
     4681 tcgcgaagat gttgctcatg tggcgggcca ccgtcttctc gctgatcacg agctcctcag
     4741 cgatcgcgcg attcgtcttg ccggccgcga caagcgtgag cacttcgagc tcgcgcttgg
     4801 tcagaccgcc gggacgcatg gacggcgccg ggtgcgcgag cgcgtccacc gaagcaacct
     4861 ccggcgccgc ctcgagctcg gcgaacacgc cgcgggaggc gtcgaactcc gccgcggcgg
     4921 tctcctcgtc accgagcgcg cggcacgcga gaccgatgag cacgcggacg cgggcggttt
     4981 cgtacggcac gtcgagctca cgccaggcct cggccgagcg tctcaacgcg acgagggccg
     5041 ctgccgtgtc gccggcctcg agctccaccg ccccgcgaac gtggtccgcg gtcgcctcga
     5101 gcaggctgct
//
LOCUS       DBNGNK010000069         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1373480,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000069 DBNGNK010000000
VERSION     DBNGNK010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1373480"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="AC150V_01765"
     CDS             complement(<1..1346)
                     /locus_tag="AC150V_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761680.1"
                     /translation="MRRLLIAAAGWLLVLVLAGQAYAQAEPERKLAETYAPVLRLVEQ
                     QEECGSGEPYDPIDIDLILGNEEVAFRGPWDKTNLVKVAPTANDLSPSAVDYHLDFPG
                     DPLEPGCEFEAFGKRTLADSEPTTYARVSTEPGKSGKLALQYWFYYVYNDFNNKHEGD
                     WEMIQLVFDADDAEDALEQEPVATGYSQHEGAERADWGDDKLEIVDGTHPVVYPAAGS
                     HANYYRPALFLGRSAAEGVGCDDTTRPHREIRPEVAWVPFDERAYLAEYPWLGYLGRW
                     GEKQPAFFNGPTGPNEKSQWTKPITWSEEKWRDKAFAMPTGSAWNTSATDFFCQAVAG
                     GSSVLTSFVRNPWPVVIALGVLIVLALWGASRTQWASSAPLRIARRRAWGQIVAATRR
                     MYTGHFRIFVQFGLLFVPIGIVIGGLQAVLFYFSSLEAFRDSAGESNASVGWFALT"
     gene            complement(1356..2411)
                     /locus_tag="AC150V_01770"
     CDS             complement(1356..2411)
                     /locus_tag="AC150V_01770"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009402601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR3761681.1"
                     /translation="MRQRIGLQYPLVQAPMAGATTVDLVVAVSEAGGLGSLACALLDP
                     DQVRAEVEAIRSRTTRPFNLNFFCHEPPSPDPAAEARWHERFSVYREELDITPDGAAM
                     PPLTPFDDSTCAAVEELAPPVVSFHFGLPAPELLSRLQSCGCLVMSSATTVAEAVALQ
                     RQGCDVIVAQGLEAGGHRGMFLTSDPGAQVGTMALVPQVVDAVDVPVVAAGGIGDARG
                     IVAAVALGASAVQLGTAYLRCPESAISPLYRRHLAEARDDSTTLTNVFTGRPARALAN
                     RATRELGPLAEDAPAFPLAGAAMAPLRAHAEPAGSSDFSSFWAGQAAALGSAEPAGEL
                     TRRLAEETRELADRLSL"
BASE COUNT          381 a          808 c          864 g          358 t
ORIGIN      
        1 aaggtcagcg cgaaccaacc cacggacgcg ttcgactcgc ccgccgagtc cctgaaggcc
       61 tcgagcgacg agaagtagaa gaggaccgcc tgcagccccc cgatgacgat cccgatcggg
      121 acgaagagca gcccgaactg gacgaagatc ctgaagtggc ccgtgtacat gcggcgcgtg
      181 gccgcgacga tctggcccca ggctcgacga cgcgcgatgc ggagcggcgc cgacgacgcc
      241 cactgcgttc gcgacgcacc ccagagggcc agtacgatca gcacaccgag cgcgatcacg
      301 accggccagg ggttccgcac gaacgacgtg aggacggagg atccgccggc gaccgcctgg
      361 cagaagaagt cggtcgcaga ggtgttccag gcgctcccgg tgggcatcgc gaaggccttg
      421 tcgcgccatt tctcctcgct ccaggtgatc ggcttcgtcc actgcgactt ctcgttcgga
      481 ccggtcgggc cgttgaagaa agcgggctgc ttctcgcccc agcgtccgag gtacccgagc
      541 cacgggtact cggcgaggta ggcgcgctcg tcgaagggca cccaggcgac ctcgggtcgg
      601 atctcgcggt gggggcgcgt cgtgtcgtcg cagccgaccc cctccgccgc gctccgcccg
      661 aggaacaggg cgggcctgta gtagttcgcg tgcgagccgg cggccggata gacgaccggg
      721 tgagtgccgt ccacgatctc gagcttgtcg tcaccccagt cggcgcgctc ggctccctcg
      781 tgctggctgt agcccgtcgc gaccggctcc tgctcgaggg cgtcctcggc gtcgtcggcg
      841 tcgaagacga gctggatcat ctcccagtcg ccctcgtgct tgttgttgaa gtcgttgtag
      901 acgtagtaga accagtactg gagcgccagc ttccccgact tgccgggctc ggtgctcacg
      961 cgggcatagg ttgtcggctc gctgtcggcc aacgtgcgct tgccgaaggc ctcgaactcg
     1021 cagcccggct cgagcgggtc gcccgggaag tcgaggtgat agtcgactgc gctcgggctg
     1081 aggtcgttcg cggtgggcgc gaccttgacg aggttcgttt tgtcccacgg tcctcggaac
     1141 gcgacctcct cgttgccgag gatcaggtcg atgtcgatcg ggtcgtacgg ctcgccggag
     1201 ccgcattcct cctgctgctc caccagcctc aggacgggcg cgtacgtctc ggccaacttc
     1261 cgctcgggct cagcttgcgc atacgcctgg ccggcgagga cgaggacgag tagccacccc
     1321 gcggccgcga tcagaaggcg tcgcactgcg agaccttaga ggctgagccg gtcggccagc
     1381 tcgcgtgtct cctccgccag gcgtcgcgtc aattcgccgg ccggctctgc ggagcctagt
     1441 gcggcggcct ggcccgccca gaaggagctg aagtccgaag accccgccgg ctcggcgtgc
     1501 gcgcgaagcg gcgccatcgc ggcgcctgcc agagggaacg ccggcgcgtc ctctgcaagc
     1561 ggtccgagct cacgcgtcgc ccggttggcg agcgcgcgag cgggccgtcc ggtgaagacg
     1621 ttggtgagag tcgtcgagtc gtcccgcgcc tcggcgagat gacgtcggta gagaggagag
     1681 attgcagact cgggacaccg gaggtacgcg gtgccgagct gaactgcgga cgcgccaaga
     1741 gccaccgcgg cgacgatccc gcgggcatcg ccgatgccgc ccgccgcgac gaccggcaca
     1801 tcgacggcgt cgacgacctg aggaacgagg gccatcgtcc ccacttgcgc ccctgggtcg
     1861 gaggtaagga acatccctcg gtgcccaccc gcttcgaggc cctgcgcgac gatcacgtcg
     1921 cagccctgcc gctggagcgc gacagcttcg gccacggtcg tcgccgagga catgaccaga
     1981 cagccgcacg actggaggcg cgagagcagt tcgggtgcag ggagtccgaa gtggaagctc
     2041 acgactggcg gcgccagctc ttcgacggcg gcgcaggtcg agtcgtcgaa cggcgtcagc
     2101 gggggcattg cggcaccgtc gggagtgatg tcgagctcct ccctgtagac ggagaagcgc
     2161 tcgtgccagc gggcctccgc ggccgggtcg ggcgagggag gctcgtgaca gaagaagttc
     2221 aggttgaagg ggcgtgtggt ccgactgcgg atcgcctcga cttccgcgcg aacttggtcg
     2281 gggtcgagga gcgcgcacgc gagcgagccg agcccgcccg cctcggagac cgcgaccacg
     2341 agatcgacgg ttgtggcgcc cgccatcggc gcctggacga gcgggtactg gaggccgatt
     2401 cgctgacgca g
//
LOCUS       DBNGNK010000070         5466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1444852,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000070 DBNGNK010000000
VERSION     DBNGNK010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1444852"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="AC150V_01775"
     CDS             complement(<1..1422)
                     /locus_tag="AC150V_01775"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3761682.1"
                     /translation="MSDEMREELEDLSVFDHRKGRKDFLFDSAKLMAAAAAAGPFFMA
                     ADQAKAAITASTSATQGDPIAKSAIDAAKQFAGSTLRKTNEAGLQALDDKNFTGPLFE
                     KLTGVKVEVVEKPFPQIFSTVIAEHIAKSGAIDVVDGSPVWIPDFAERGVIDPVDSFI
                     RKYKAQSSNNDIHPLYRPLHSYKGKQYGYFDDGDIWILYYRKDIFNNAALKRAHQAKF
                     GKPLRVPRTWDEFSQTAQFITDELGPKVYGTSMGRALGNPGNQFYFYQQFRSNGGQFF
                     NPNTMKALINNKIGVRTMNQILAQNAASSPGIEKLDFVSGWVQWLEGKTAMMMAWPPT
                     GRISENYAQRDKGFAFLPKSKIVGKTGYAIAPGQNGEHAGAFVKSVMATGKQKELAYL
                     FTQWATSPSISLQRVQLPYTLRDPYRISHYRSKQFAKRWPGAKQYLQKLCEGANFGVL
                     DPVYTGSQDYANALDRGMTKIYAG"
     gene            complement(1568..2779)
                     /locus_tag="AC150V_01780"
     CDS             complement(1568..2779)
                     /locus_tag="AC150V_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761683.1"
                     /translation="MAQVSFDHVRKVYDDGTVAVQDLSLEIPDGELMVLVGPSGCGKT
                     TALRMVAGLEVITQGEIRIGEDVVNKLPPRDRDVAMVFQNYALYPHKSVYENLSFPLK
                     LRRMSRSDINERVNRIAGVLELEEQLKRKPRQLSGGQRQRVAMGRAMIREPRAYLMDE
                     PLSNLDAKLRTQMRAEIGLLHKELGVTMIFVTHDQIEAMTLGERVAVMRKGELQQVAT
                     PPELYNQPTNLFVAGFIGSPGMNFFEGKVETGGEGVMVAFGDGQRLRVDETELAFAGS
                     LAQRNGAGVVVGVRPEHLEDGALSTDTPTERRLHGLVRLRELLGSEIVIHFEVEAAPV
                     VSDEVREIAADVDPSALTELDHMRSAHRTSFVGRFGADSQTREGEVAEIAVSPGALRF
                     FDSETGARIGA"
     gene            2950..3708
                     /locus_tag="AC150V_01785"
     CDS             2950..3708
                     /locus_tag="AC150V_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3761684.1"
                     /translation="MGDANPEPRPREAEGALFEQVAREPRLSDKVAEMMLETILSNRL
                     EVGDRLPSERELGEQFGVSRTVIREAVRALVAKGVIEVRSGSGLRVAAVDASAVSESM
                     ALYVRGGRLDFGKVHEVRRELEVHIAGLAAERASEEDIARLREVHDRMVKAVGDLDSA
                     ALDDLEFHRRIALTTHNELFLVLMDSIGRSLIDIRRANLGSGSAPETLAQHEAVLEAI
                     AANDPEAARVAMAAHLDGVAEWYHEHESAEVPAT"
     gene            3705..4139
                     /locus_tag="AC150V_01790"
     CDS             3705..4139
                     /locus_tag="AC150V_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3761685.1"
                     /translation="MTLLRRLDHVAILVRDTDDALRFYRDHLGLSVHSSEEIPTPHVR
                     LTYLDAGNAYVQLVEPLDPDGPLAVALAANGEGLHHICFGVDDVGEAVAALSDEGSDV
                     ALGSGRGRVSGFVTTNGGHGVRIECTEFDRAQDVDAAAGWLG"
     gene            complement(4254..4541)
                     /locus_tag="AC150V_01795"
     CDS             complement(4254..4541)
                     /locus_tag="AC150V_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761686.1"
                     /translation="MFTTANKIKKVALVGTVVAAIAVPAALAQTDGGILTKASRQSTP
                     SYAGMVTDGTLLTQASRTQAAQEALEATYAGMVTDGALLTAAAKKAEPLAR"
     gene            4655..>5466
                     /locus_tag="AC150V_01800"
     CDS             4655..>5466
                     /locus_tag="AC150V_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3761687.1"
                     /translation="MEFRILGPVEVVDGDRPLDLGGEKLRALLVLFLLHANEVMSRDR
                     LLDELWGDEQPRSGSTALRVRVAELRKRLGADRVETRAPGYAFKLKPEELDLSRFEQL
                     VETAERAEPAEALRLLDEALGLWRGAPLADVAYERYAQAPIGRLEELRLAAVERRVEA
                     RLALGADDELLGELRELVAEHPFREQLRAQRMLALYRSGRQAEALEEYREVHKLLVDE
                     LGIDPGPALQELEKRILRQDPDLAGDARGTEPARKVLLVSTSEAGLDALVALV"
BASE COUNT          805 a         1830 c         1875 g          956 t
ORIGIN      
        1 gcccgcgtag atcttcgtca tgccgcggtc gagcgcgttt gcgtagtcct gcgagcccgt
       61 gtagacgggg tcgagcacgc cgaagttcgc gccctcgcag agcttctgca ggtactgctt
      121 cgcacccggc caccgctttg cgaactgctt ggagcggtag tgagagatgc ggtacgggtc
      181 tcggagggtg tagggcagct gcacccgctg gagcgagatc gacgggctgg tcgcccactg
      241 cgtgaacaga taggcgagct ccttctgctt gcccgtcgcc atcacggact tgacgaacgc
      301 gccggcgtgc tcgccgttct gacccggcgc gatcgcgtag cccgtcttgc cgacgatctt
      361 cgacttcggc aggaaggcga accccttgtc gcgctgggcg tagttctccg agatccggcc
      421 ggtgggcggc caggccatca tcatcgccgt cttcccctcc agccactgca cccagccgga
      481 cacgaagtcg agcttctcga tgccggggga cgacgctgcg ttctgcgcga ggatctggtt
      541 catcgtccga acgccgatct tgttgttgat cagcgccttc atcgtgttcg ggttgaagaa
      601 ctggccaccg ttcgagcgga actgctggta gaagtagaac tggttgcccg ggttgccgag
      661 cgcccggccc atgctcgtgc cgtacacctt cggtcccagc tcgtccgtga tgaactgggc
      721 cgtctggctg aactcgtccc aggtccgcgg cacccgtagc ggcttgccga acttggcctg
      781 gtgcgccctc ttcaacgcgg cgttgttgaa gatgtccttg cggtagtaga ggatccagat
      841 gtcgccgtcg tcgaagtacc cgtactgctt gcccttgtag ctgtgcagcg ggcggtagag
      901 cgggtggatg tcgttgttcg acgactgtgc cttgtacttc ctgatgaagc tatcgaccgg
      961 gtcgatgacg ccccgctcgg cgaagtccgg gatccagacg ggtgagccgt cgacgacgtc
     1021 gatcgcgccg gacttggcaa tgtgctcggc gatcaccgtc gagaagatct gcgggaacgg
     1081 cttctcgacc acctccacct tcactccggt cagcttctcg aacagcggac ccgtgaagtt
     1141 cttgtcgtcg agtgcctgaa ggcccgcctc gttcgtcttc ctcagcgtcg agccggcgaa
     1201 ctgcttggcg gcgtcgatcg cgctcttggc gatcgggtcg ccctgagtag cgctggtcga
     1261 tgccgtaatg gccgctttgg cctgatcggc agccatgaag aatggccctg ccgcagccgc
     1321 ggcggccatg agcttggcgc tgtcgaagag gaagtccttg cggcccttgc ggtggtcgaa
     1381 gaccgaaagg tcttcgagct cctctctcat ctcgtcactc accgctcctg gctcctttct
     1441 gatggcctgc tgtatgaccg gatgcgatca tccccttttc gggcaggcat cgtcaagtgg
     1501 cgaccgcgcc gcgcgcggcc tatgacccct agttagcaat gaacggaacc agaaccggtt
     1561 cccgacgtca agccccgatg cgcgcgcccg tctccgagtc gaagaaacgg agcgctcccg
     1621 gcgagaccgc gatctcggcc acctcgccct cgcgcgtctg cgagtcggcg ccgaagcgcc
     1681 cgacgaagga cgtccggtgc gcggagcgca tgtgatcgag ctcggtcagc gcggacgggt
     1741 cgacgtccgc ggcgatctcg cggacctcgt cactcacgac gggcgccgcc tcgacctcga
     1801 aatggatgac gatctcggag ccgagtagct cccgcagccg gacgaggccg tggaggcgac
     1861 gctcggtcgg agtgtcggtg gagagtgcgc cgtcctcgag gtgctcgggc cgcacgccga
     1921 cgacgacgcc ggcgccgttt cgctgggcca atgacccggc gaaagcgagc tcggtctcgt
     1981 ccacgcgcag ccgctgcccg tccccgaagg cgaccatgac gccctcgccg cccgtctcga
     2041 cctttccctc gaagaagttc atgccggggc tgccgatgaa gccggcgacg aacaggttcg
     2101 tcggctggtt gtacagctcg ggtggcgtcg cgacctgctg cagctcgccc ttgcgcatca
     2161 ccgccacgcg ctcgccgagc gtcatcgcct cgatctggtc gtgggtcacg aagatcatcg
     2221 tgacgccgag ctccttgtgg agcagcccga tctcggcgcg catctgggtg cggagcttcg
     2281 cgtcgagatt cgagagcggc tcgtccatca ggtacgcccg cggctcgcgg atcatcgccc
     2341 ggcccatcgc gactcgctgg cgttgcccac cggacagctg gcgcggcttg cgcttcagct
     2401 gctcctcgag ctcgagcacg cccgcgatcc tgttgacgcg ctcgttgatg tcgcttctcg
     2461 acatccgccg cagcttgagg gggaaggaga ggttctcgta gaccgacttg tggggataga
     2521 gagcgtagtt ctggaacacc atcgccacgt cgcggtcgcg cggcgggagc ttgttcacga
     2581 cgtcttcgcc gatcctgatc tcgccctgtg tgatcacctc gagcccggcc accatgcgca
     2641 gcgcagtggt cttgccgcag cccgaggggc cgacgagcac catcagctcg ccgtcgggga
     2701 tctccagcga caggtcttgg acggcgaccg tgccgtcgtc gtagaccttc ctgacgtgat
     2761 cgaacgaaac ctgtgccacc gacgccctct tctatatcac tgctggcgag actgctctac
     2821 atgactgctg gagactgcgc ggcccgcgcg accgctgcgg ccgcgcgaat cgtgcccgcg
     2881 gtgaacctat acgacagcgt accgaggagc gaatcgaacc ggcatgccgt atgacgtatg
     2941 atcgattgca tgggtgacgc gaatcccgag cctcgtcctc gagaagcaga aggtgcgctg
     3001 ttcgagcagg tcgcccggga gccgcgactc tccgacaagg tcgccgagat gatgctcgag
     3061 acgattctct cgaaccggct cgaggtcggc gaccggttgc cgtccgagcg tgagctcggc
     3121 gagcagttcg gggtgtcgcg cacggtgatt cgagaggcgg tgcgcgctct tgtggcgaag
     3181 ggcgtgatcg aggtgcgctc ggggagcggg ctgcgcgtcg ccgcggtgga cgcgtcggcc
     3241 gtgagcgagt cgatggccct atacgtgcgt ggcggacggc tcgacttcgg gaaggtgcac
     3301 gaggtccggc gggagctcga ggtgcacatc gccggtcttg cggcggagcg cgcgagcgag
     3361 gaggacatcg cgcggctccg cgaggtgcac gaccggatgg tgaaagcggt gggcgacctc
     3421 gactcggcgg ccctggacga cctcgagttt caccgtcgca tcgcgctcac gacgcacaac
     3481 gagctctttc tcgtgctgat ggactcgatc ggacgctcgc tgatcgacat tcgacgggcg
     3541 aacctcggct cgggctcggc tcccgagacc ctggcgcagc acgaggcggt gctcgaggcg
     3601 atagccgcca acgatcccga ggctgcccgc gtcgcgatgg ccgcccatct ggacggcgtt
     3661 gcggagtggt accacgagca cgagtccgcg gaggtgccgg cgacatgacc ctgcttcggc
     3721 gtctcgacca tgtcgcgatc ctcgtgcgcg acaccgacga tgcgctccgc ttctatcgcg
     3781 atcacctcgg gctgtccgtc cactcgagcg aggagatccc gaccccgcac gtccggctca
     3841 cctacctcga cgcgggcaac gcttacgtcc agctcgtcga gccactcgac ccggacggcc
     3901 cgctcgccgt agcgctcgcc gcgaacggcg aaggcctcca tcacatctgc ttcggcgtcg
     3961 acgacgtcgg cgaggcggtc gccgcgctga gcgacgaggg ctccgacgtc gccctcggct
     4021 cgggtcgcgg tcgcgtgtcg ggcttcgtca cgacgaacgg cggccacggc gtgcggatcg
     4081 agtgcacgga gttcgaccgc gcgcaagacg tcgacgcggc cgccggctgg ctcggctaac
     4141 cgcgcaccgg caagacggcg acgacctcgg cccctgccca ggccgtcgcc gcggccgcag
     4201 cgctggccgc ggccgcagcg acgccgatcg cggggtcgag atccccatag ccgctaccgg
     4261 gccagcggct cggccttctt ggcggcggcg gtgagcagcg cgccgtccgt gaccatgccg
     4321 gcgtaggtgg cctcgagcgc ctcctgggcc gcctgggtcc ggctggcctg cgtcagcagg
     4381 gttccgtcgg tgaccatgcc ggcgtaggac ggtgtcgatt gccggctggc cttcgtgagg
     4441 atcccgccgt cggtttgcgc gagcgccgcc gggacggcga tcgccgcgac gactgtgccg
     4501 acgagagcta ccttcttgat cttgttggcc gtggtgaaca tgtctttcct tcctgtccgg
     4561 gccacttccg tgtggccatc gcggacaggg tcgagcgcgg ccgtaagcgc gctgtaacaa
     4621 cggggttaca ggccctgtta acgtcggctg cgccgtggag ttcaggatcc tcggaccggt
     4681 cgaagtcgtg gacggtgacc gcccactgga tctcggcgga gagaagctgc gcgccttgct
     4741 cgttctcttc ctcctccacg ccaacgaggt catgtcccgc gaccgcctac tcgacgagct
     4801 gtggggcgac gagcagccgc ggagcggctc gaccgcgctc cgcgttcggg tcgcggagct
     4861 gcgcaagcgg ctcggcgccg accgggtcga gacgcgcgcg cccggctacg cgttcaagct
     4921 caagccggag gagctcgacc tgtcgcgctt cgagcagctc gtcgagaccg cggagcgggc
     4981 cgagccggcc gaagcactgc gcctgctgga cgaggccctc ggattgtggc gaggtgcccc
     5041 gctcgccgac gtcgcctacg agcggtacgc ccaggctccg atcgggcgtc tcgaggagct
     5101 ccggctcgcg gcggtcgagc gccgcgtcga ggcgcggctc gcgctcgggg cggacgacga
     5161 gctcttgggg gagctgcgcg agctcgtcgc tgagcatccg ttccgggagc agttgcgcgc
     5221 ccagcggatg ctggcgctct accggtcggg gcggcaggcg gaggcgctag aggagtaccg
     5281 cgaggttcac aagctgctcg tcgacgagct ggggatcgat cccggcccgg cgctgcagga
     5341 gctcgagaag cggatacttc gccaggatcc cgaccttgca ggggatgctc gcggcaccga
     5401 gcccgcacga aaggtgctgc tcgtctccac ttccgaagcg gggctcgacg ccctcgtcgc
     5461 tctcgt
//
LOCUS       DBNGNK010000071         7934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_728089,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000071 DBNGNK010000000
VERSION     DBNGNK010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7934
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_728089"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..713)
                     /locus_tag="AC150V_01805"
     CDS             complement(111..713)
                     /locus_tag="AC150V_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR3761688.1"
                     /translation="MGYQVEGSLLEVCTCDVLCPCWVGEDPDNGTCDGVLSWHIDKGS
                     SNGTDLSGRTITLLAHIPGNILEGNWRAVVYLDDGCTPEQEQAILDIWTGKMGGPIAD
                     LAQLVGEVVAVEKQPVSFQVEGKGVAGRLSIGRAISAELEPFTGATGEPSALHDSIFT
                     TIPGSPAYVGKASSYKVDAHGFEIDLEGHNAVSGRFRFEG"
     gene            complement(818..1204)
                     /locus_tag="AC150V_01810"
     CDS             complement(818..1204)
                     /locus_tag="AC150V_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761689.1"
                     /translation="MSEMDEQQGQEADPPAEVQDAAAREKGAFEKATDAGQQALNDLG
                     DAVDDLVAEAPDLWEKTKTAAGGVADEVKEKGPGIWDQMKGLASDAAKRFDEGFKGEA
                     SGAAKPDEQDAAAPTSGDGDQTDDRG"
     gene            complement(1230..2111)
                     /locus_tag="AC150V_01815"
     CDS             complement(1230..2111)
                     /locus_tag="AC150V_01815"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HIR3761690.1"
                     /translation="MSVPVFRTPDDRFVDLPGWPFPPRYVEQDGLRMHFVEHGHGDPI
                     LLLHGEPTWGYLYRKMIPRLSRDGRVVVPDLFGFGRSDKPTERDWYSYDAHYGSIERL
                     VVEQLDLRSLTIVVQDWGGPIGFRLAVEHPELVDRLVVLNTGIGARAPSDEWLRFRDF
                     MRRVDTEIVPGQLVKISCATELPDAVVDAYNAPFPVPESRTGVVMFPELVATSADHPS
                     GAAMIAVREKLREWSKPTLVLFSDSDPIFTTALAERMAELIPGAGPAQTVSGAAHFLQ
                     EDKGEEIADRIGNWFFE"
     gene            complement(2145..2891)
                     /locus_tag="AC150V_01820"
     CDS             complement(2145..2891)
                     /locus_tag="AC150V_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3761691.1"
                     /translation="MTLTERQQELCESSRWDFSDLRAAYVNCTLKPSPELSHTQGLMD
                     VSIAIMEANGVSVETIRAVDHDLAPGVYPDMTEHGAASDAWPELYERVGTADILVLGT
                     PIWLGEKSSVCTRVVERLYGCSSLLNDEGQYAYYGKVGGCLVTGNEDGVKHCAMNVLY
                     SLQHLGYTIPPQADAGWIGEAGPGPSYLDAGSGGPDNDFTQRNTTFMTWNLLHTARLL
                     KDAGGFPAHGNQRSLWDAGCRFDHPNPDYR"
     gene            2963..4357
                     /locus_tag="AC150V_01825"
     CDS             2963..4357
                     /locus_tag="AC150V_01825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3761692.1"
                     /translation="MTASETSALFDALVEALGGDASRVADGDSERDLHAADLTFHEPR
                     LPDVVVYPTSTAEVARVLALADELRVPVTPFAAATSLEGHVIPLAGGISLDLTRMDKV
                     VAIEPENMAATVQPGVTRSALNRAGGQHGLFFPVDPGADATIGGMAATNAAGTTTPRY
                     GKMRPNVLALEAVLPGGSVIRTGSRALKTSAGYDLTGLLVGSEGTLGVITEVTVRLFG
                     IPEHAVAVRISFPSVEAAARAAVAAVAVGAGVTRLELLDAMNITAINAYSGTTYPEVP
                     CLFVEATGTEASVEGDLELMLALAEEEGAVEVAHEREADARSQLWSARHNAAYSWSRL
                     WPGRRHCSTDVAVPLSELPAAIAVAREEVERLGLRGGILGHVGDGNFHVGAMVDPENV
                     DEIAAFAELSRILVEDAIARGGTSTGEHGVGAGKLRALEREHGDLLPLMRGIKSQFDP
                     NGIMNPGKVLSAES"
     gene            complement(4365..4592)
                     /locus_tag="AC150V_01830"
     CDS             complement(4365..4592)
                     /locus_tag="AC150V_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761693.1"
                     /translation="MTTTAFVEALLSGQRIALADEADDAVAWRFEQLERAGFDDMPAL
                     AIAVDSDIDLHAACDLLRRGCPQTVALEILF"
     gene            4724..6040
                     /locus_tag="AC150V_01835"
     CDS             4724..6040
                     /locus_tag="AC150V_01835"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HIR3761694.1"
                     /translation="MSASALDAVAARPAAGGRSATPASLGRRALSFLVDCALLGGILA
                     WIVLMEFVFSGELEALQWLLLVWIAVVGPLYFGLYHAYGTGATPGQLDLGIAVRDAAT
                     DGLVGTGRGVARAYLGLVVPIVALDVLVTTSAGRERMLRDAWTRTSIVSAPDLIRGTH
                     AVEPTEPEVEAYFESDRGEFLGRAISLIGAFPGALIGSVVAVYLALIAIIAVLAPLFM
                     ADFSADDWDGLYALYAWLEVALITFVGGVFWSQATIVTAAELIRTGRKASLRTVLGTV
                     VERVNALAAAALILALLVVVAASLWVTVVAVLAALFLISRMTLVVPAIAIESRSVSDA
                     IVRSWELTRGRTWQLLGMVLGSVALVAVAVGLAVGLAVVLARAVDPEAPPLAWALVIA
                     VGMGLGSLLTGAILAWLGTGWFLVYHDARRSVAQATKEVGQDAPAA"
     gene            6024..6719
                     /locus_tag="AC150V_01840"
     CDS             6024..6719
                     /locus_tag="AC150V_01840"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3761695.1"
                     /translation="MLPLLDLGLPRRIPRLTLALVLANVAVWLLYQVPSGIEHSVDLL
                     GFRACSVEGSCPSDPDLIWPVTLFTAMFAHGGWSHLIGNMLFLLVFGPRVEADLGAFR
                     YAAVYVVAGLGAEALFVGTTLSFAPAADAAIPGIGASGAISGVLGAYLIAHPFQRVLV
                     WAMPVLFLRVPALGLLGVWLLLQTLEGAYALSHPDALVGVAFMAHVGGFVVGAAAGLL
                     ARGRYSPVVWRHR"
     gene            complement(6716..7738)
                     /locus_tag="AC150V_01845"
     CDS             complement(6716..7738)
                     /locus_tag="AC150V_01845"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR3761696.1"
                     /translation="MAMSELYETAAAIVADDRGILAADESTGTIKKRFDSIGVESTEE
                     SRRFYRNMLFTTPGMEDFIGGVIMYDETIRQASDDGTPFAELLAGKGVVPGIKVDTGA
                     KDLALHGEEKVTEGLDGLRERCTEYYGLGARFAKWRAVITIGDGIPTDACIDANAHAL
                     ARYAAICQETGLVPIVEPEVLMDADNTIETCYEVTARTLQSVYEALYVQGVDLRGTLL
                     KPNMVIAGKGCSTQASARQIAELTVRCFLDHVPARVPGIVFLSGGQSEVQATENLNEI
                     NRVGGPWPLSFSYGRALQASALAAWGGDAANADAAQAAFAHRARMNSLAVAGEWTADV
                     EEAVTA"
     gene            complement(7725..>7934)
                     /locus_tag="AC150V_01850"
     CDS             complement(7725..>7934)
                     /locus_tag="AC150V_01850"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HIR3761697.1"
                     /translation="HDDLVRGENVFVSATGVTSGALLRGVRLTGAGVETESIVMRSRS
                     GTVRRIAAYHPAEKIRELRKGFGDE"
BASE COUNT         1140 a         2785 c         2632 g         1377 t
ORIGIN      
        1 gggcgagcca ggccgccagc acgagcgaac cgacaagccc caccagcagg cgctgatcag
       61 cgattcgtgg ccacaggcgc cctcttctgc ccttcgctgg gccgttccgc tcagccttcg
      121 aagcggaacc ggcccgagac cgcgttgtgg ccctcgaggt cgatctcgaa accgtgcgcg
      181 tcgaccttgt agctcgacgc cttgccaacg taggccggcg agccggggat cgtcgtgaag
      241 atgctgtcgt ggagcgccga cggctctccc gtcgcgccgg tgaagggctc gagctccgcc
      301 gagatggcgc gcccgatcga caggcggccg gccaccccct tcccttcgac ctggaacgac
      361 acgggctgct tctcgacggc gacgacctca ccgaccagtt gtgcgagatc ggcgatcggg
      421 ccgcccatct tcccggtcca gatgtccagg atggcctgtt cctgctccgg cgtgcagccg
      481 tcgtcgagat agaccacggc ccgccagttc ccctccagga tgttgcccgg gatgtgcgcc
      541 aggagcgtta tcgtccgacc cgacaggtcg gttccgttcg agctgccctt gtcgatgtgc
      601 caggacagga cgccgtcgca cgtgccgttg tcgggatcct cgccgaccca gcacggacac
      661 aagacgtcgc acgtgcagac ttcgagcagg cttccctcga cctgatagcc catttctcct
      721 ccctatggtc gactgcaacc ggtctgagcg gccataccct acgctcgcgt tgcaactgat
      781 acaaggccgc gtgccacgcg gccgagggcg tctccggtca gccgcgatcg tctgtttggt
      841 cgccgtcgcc ggaggtcggc gcggctgcat cctgctcgtc gggcttcgcg gcgccggagg
      901 cctcgccctt gaagccctcg tcgaagcgct tcgccgcatc cgaggccaag cccttcatct
      961 gatcccagat gcccgggccc ttctccttca cctcgtcggc gaccccaccg gcggccgttt
     1021 tcgtcttctc ccagaggtcc ggagcttcgg cgacgaggtc gtccacggca tcgccgagat
     1081 cgttcagcgc ctgctgaccg gcgtccgtcg ccttttcgaa agctcccttc tcacgggccg
     1141 cggcgtcctg cacctccgcg ggaggatccg cctcttgacc ctgttgctcg tccatctcgc
     1201 tcatcggtcc gcctcctccg tctctggcgt cattcgaaga accagttgcc gatgcgatcg
     1261 gcgatctcct cgcccttgtc ctcctgcagg aagtgtgccg cccccgagac cgtctgagcc
     1321 ggccctgccc ccgggatcaa ctcggccatg cgctccgcca gggcggtcgt gaagatcgga
     1381 tccgagtcgc tgaagagcac gagcgtcggc ttgctccatt ccctcagctt ctcccgcacg
     1441 gcgatcatcg cggcgccgct cgggtgatcg gcagacgtgg caaccagctc ggggaacatg
     1501 acgacgccgg ttctggactc gggcaccggg aagggggcgt tgtaggcgtc gacgacggcg
     1561 tcgggcagct ccgtcgcgca ggagatcttg acgagctgcc ccggaacgat ctcggtgtcg
     1621 acgcggcgca tgaagtcgcg gaagcggagc cactcgtcgg acggcgcgcg ggcgccgatg
     1681 cccgtgttga ggacgacgag ccggtcgacg agctcggggt gctcgaccgc caggcggaag
     1741 ccgatagggc caccccagtc ctgcaccacg atcgtgagcg agcgcaggtc gagctgttcg
     1801 acaacgaggc gctcgatcga gccgtagtgc gcgtcgtagc tgtaccagtc acgctctgtc
     1861 ggcttgtccg accgcccgaa gccgaagagg tccggcacga cgacccgacc gtccctgctc
     1921 aaccgcggaa tcatctttcg atacaggtac ccccaggtcg gctcgccgtg cagcaacagg
     1981 atcgggtcac cgtgcccgtg ctcgacgaag tgcatgcgca gcccgtcctg ctcgacgtag
     2041 cggggcggga acggccaccc cggcagatcg acgaagcggt cgtccggagt cctgaagacc
     2101 ggcaccgaca cggagtcagc ctatgcgttc cgttccgccg gagctcaccg gtagtccggg
     2161 ttcggatggt cgaagcgaca acccgcgtcc cagagggatc gctgattgcc gtgggcggga
     2221 aagcctccgg cgtccttcag caggcgggca gtgtgcagga gattccacgt catgaaggtc
     2281 gtgttgcgtt gggtgaagtc gttgtcgggc ccgccgctgc ccgcgtcgag gtacgacggc
     2341 cccggcccgg cctcgccgat ccaacccgca tcggcctgcg gcggaatcgt gtagccgaga
     2401 tgctggaggg agtagagaac gttcatcgcg cagtgcttga cgccgtcctc gttgcccgtg
     2461 acgaggcagc ccccgacttt cccgtagtac gcgtactgac cctcgtcgtt gaggagcgac
     2521 gagcagccgt acagacgctc taccacccgg gtgcagaccg aggacttctc gccgagccag
     2581 atcggcgtgc ccaggacgag gatgtccgcc gtcccgaccc gctcgtaaag ctcgggccaa
     2641 gcgtcgctcg ctgcaccgtg ctcggtcatg tcggggtaca cgccgggcgc caggtcgtgg
     2701 tcgacggcac ggatggtctc gacggacacg ccgttcgctt ccatgatcgc gatcgagacg
     2761 tccatgagcc cctgcgtgtg ggacagctcg ggggacggct tgagcgtgca gttgacgtac
     2821 gctgcccgga gatcgctgaa gtcccagcgg ctcgactcgc aaagctcctg ctggcgctcg
     2881 gtcagggtca tgcggctcct cctcgttcgt ggtcgctcac atcgtcccac gccggcgccc
     2941 ggtttcgggc agactcgtct gagtgaccgc ctccgagact tccgcactct tcgacgcgct
     3001 ggtcgaggcg ctgggcgggg acgcctcccg cgtcgccgac ggcgactccg agcgcgacct
     3061 tcacgccgcc gatctgacgt tccacgagcc ccgactgccc gacgtcgtcg tgtatccgac
     3121 ctcgaccgcc gaggtcgcgc gtgtcctggc actggcagac gagctgcgcg tcccggtgac
     3181 tcctttcgcg gcagcgacga gcctcgaggg gcacgtgatt ccactcgccg gcgggatcag
     3241 cctcgacttg acaaggatgg acaaggttgt cgctatcgag cccgagaaca tggcggcgac
     3301 agtgcagccc ggcgtgaccc gttcggcgct gaatcgcgcc ggcggccagc acggactctt
     3361 cttcccggtc gatccgggcg ccgacgcgac gatcggcggc atggccgcca cgaacgcggc
     3421 gggaacgacg actccccgct acgggaagat gcggccgaac gttctcgcac tggaggcggt
     3481 gctgccgggc gggagcgtaa tccgcacggg cagccgtgca ctgaagacct ctgcaggcta
     3541 cgacctgacg gggctcctgg tcggctcgga agggacgctc ggggtgatca cggaggtcac
     3601 cgtccggctc ttcggaatcc ccgagcacgc cgtcgcggtc cggatctcgt tcccgagcgt
     3661 cgaggccgcc gcgcgggcgg cggtcgcggc ggtggcggtc ggcgcaggcg tcacgagact
     3721 cgagctgctc gacgcgatga acatcacggc gatcaacgcc tactcgggaa ccacgtaccc
     3781 cgaggtgccc tgcctcttcg tcgaagcgac cggcacagag gcctcagtgg aaggcgatct
     3841 cgagctgatg ctcgcgctcg ccgaggagga aggcgccgtc gaggtggccc acgaacgcga
     3901 ggcagacgct cgctcgcagc tctggtcggc gcgacacaac gccgcgtaca gctggtcgcg
     3961 cctctggccc ggccgccgtc attgctcgac ggacgtcgcc gttcctctct cggagcttcc
     4021 cgccgcgatc gcggtggcgc gggaagaggt cgagcggctc ggcctccgcg gcgggatcct
     4081 cggccacgtg ggcgacggga acttccacgt cggcgcgatg gtcgatccgg agaacgtgga
     4141 cgagatcgcg gccttcgcag agctctcacg gatccttgtc gaggatgcga tcgcccgcgg
     4201 cggcacgagt accggcgagc acggagtcgg cgccggcaag cttcgcgctc tggagcgcga
     4261 gcacggagac ctgctgcctc tgatgcgggg catcaagtcc cagttcgacc cgaacgggat
     4321 catgaatccc ggcaaggtgc tctcagccga gagctgagcg ggttctagaa gaggatctcg
     4381 agcgcgacgg tctgcgggca gccgcggcgg agcaggtcgc aggcggcgtg gagatcgatg
     4441 tcggaatcca ctgcgatcgc cagtgccggc atgtcgtcga agccggcccg ttcgagctgc
     4501 tcgaagcgcc acgccacggc gtcgtccgcc tcgtcggcga gagcgatccg ctgaccgctg
     4561 agaagcgctt cgacgaatgc cgtggtcgtc acgagtactc atttcggcac gaagcgccga
     4621 tcccttgaac gagtgatgag cggattcccc gacacctgat cagcacgacc ccggattcca
     4681 cgcaccggcc cgatccgcag actttgaggc gaaaggagca caggtgtcgg cgtcagccct
     4741 tgacgcagtg gcagccaggc ctgccgccgg tgggcgatcg gcaacgcccg cgagcctcgg
     4801 ccggcgcgcg ctctccttcc tcgtcgactg cgctctgctc ggcgggatcc tcgcgtggat
     4861 cgtgctcatg gagttcgtgt tctccggcga gctcgaggcg ttgcagtggc tgcttctcgt
     4921 ttggatcgcg gtcgtcgggc cgctctactt cggcctctac cacgcctatg gaaccggcgc
     4981 gacgccaggc cagctcgacc ttggcattgc cgtgcgtgac gctgcgaccg acggcctggt
     5041 cggaacgggc cgcggggtcg cgcgggcgta tctcggcctc gtcgtgccca tcgttgcgct
     5101 ggacgtgctc gtcacgacgt ctgccggccg cgagcgcatg cttcgtgacg cgtggacgcg
     5161 cacgagcatc gtctcggctc ccgacctcat ccggggcacg cacgcggtcg agccgaccga
     5221 gccggaggtc gaggcgtact tcgagtcgga ccgaggtgag ttcctcggac gggccatctc
     5281 gctcatcggt gcgttccccg gcgctctgat cggctccgtc gtcgccgtct atctcgcgct
     5341 gatcgcgatc atcgcggtgc tcgcaccgct cttcatggcc gacttctcgg ccgacgactg
     5401 ggacggcctc tacgcgctgt acgcgtggct cgaggtcgca ctgatcacgt tcgtcggcgg
     5461 cgtcttctgg tcgcaggcca cgatcgtgac cgcggcggag ctgattcgta cgggccggaa
     5521 ggcgtcgctc cggaccgtcc tcgggacggt ggtcgagcgc gtcaacgcgc tcgccgcggc
     5581 ggcgctcatt cttgcgctgc tcgtcgtagt cgcggcaagc ctctgggtga cggtggttgc
     5641 cgtccttgca gcactgttcc tgatcagccg catgacgctc gtcgttccgg ccatcgcgat
     5701 cgagagccgg agtgtcagcg acgcgatcgt gcgcagctgg gagctgacgc ggggccgcac
     5761 gtggcagctg ctcggaatgg ttctcggcag cgtcgcgctc gtcgcggtcg ccgtcggcct
     5821 cgcggtcggg ctggcggtcg tgctcgcccg cgcggtcgat cccgaagcac cgcccttggc
     5881 gtgggctctc gttatcgccg tcggaatggg cctcgggagt ctcctcaccg gggcgatcct
     5941 ggcctggctc ggtacggggt ggttcctcgt ctaccacgac gcgcggcgca gcgtggcgca
     6001 ggccacgaag gaggtggggc aggatgctcc cgctgcttga cctcggactg ccccggcgaa
     6061 ttccgcgtct cacgctggcc ctcgttttgg ccaacgtcgc cgtctggctc ctctaccagg
     6121 tgccgtcggg aatcgagcat tcggtcgacc tcctcggctt ccgcgcatgc tccgtcgagg
     6181 gctcctgtcc aagcgacccc gaccttatct ggccggtcac gctcttcacg gcgatgttcg
     6241 cccacggcgg ctggtcgcac ctgatcggca acatgctctt cctgctcgta ttcggccccc
     6301 gagtcgaggc cgatctgggg gcgttccgct acgccgccgt ctacgtcgtc gccggcctcg
     6361 gcgccgaggc cctcttcgtc ggcacgacac tctccttcgc accggccgca gatgccgcga
     6421 tcccgggaat cggagcgagc ggtgcaatct ccggcgtcct cggcgcgtat ctgatcgcac
     6481 atcccttcca gcgcgtgctc gtctgggcga tgccggttct cttcttgcgc gttcccgcgc
     6541 tcggcctgct gggggtctgg ctcctcctgc agacactcga gggcgcatac gcgctcagcc
     6601 accccgacgc tctcgtcggg gtcgccttca tggcgcacgt cggcgggttc gtcgtcggtg
     6661 cggccgcggg cctactggcc cgcggcaggt acagcccggt cgtctggcgg caccgctagg
     6721 cggtgaccgc ctcctccacg tcggccgtcc actcgcccgc gaccgccagt gagttcatcc
     6781 gcgcgcggtg cgcgaacgcc gcctgggccg cgtctgcatt cgccgcgtcg ccgccccagg
     6841 ccgcgagtgc cgaagcctgc agcgcgcgcc cgtaggagaa ggagagaggc caagggccgc
     6901 ccacgcggtt gatctcgttg agattctcgg tcgcctgcac ctcagactgg ccgcccgaga
     6961 ggaagacgat gccgggcaca cgcgccggga cgtggtcgag gaagcagcgg acggtcagct
     7021 ccgcgatttg ccgcgcgctt gcctgcgtcg agcatccctt ccccgcgatg accatgttcg
     7081 gcttcagcag cgtgccgcgc agatcgactc cctgcacgta gagggcctcg tacacggact
     7141 ggagcgtcct ggcggtcacc tcgtagcacg tctcgatggt gttgtcggca tccatcagga
     7201 cctcgggctc gacgatgggc acaagaccgg tctcctgaca gatcgccgcg taccgtgcga
     7261 gcgcgtgcgc attcgcgtcg atgcacgcgt cggtcgggat gccatcgccg atcgtgatca
     7321 ccgcccgcca cttcgcgaag cgcgcgccca gcccgtagta ctcggtgcag cgttcgcgca
     7381 agccgtccag cccctcggtc accttctcct cgccgtgcag agcgaggtcc ttggcgccgg
     7441 tgtcgacctt gatgcccggc acgaccccct tgcctgccag gagctctgcg aagggcgtcc
     7501 cgtcgtcgga cgcctggcga atcgtctcgt cgtacatgat cacgcccccg atgaagtcct
     7561 ccataccggg agtggtgaac aacatgttcc ggtagaagcg acggctctct tcggtggatt
     7621 ccactccgat cgagtcgaac cgcttcttga tcgttccggt gctctcgtcg gcggccagga
     7681 tgccccggtc gtccgcaacg atggcagcgg cggtctcgta cagctcactc atcgccaaat
     7741 cctttcctca actccctgat cttctcggcc ggatggtagg cggcgatccg tcgcacggtg
     7801 cccgagcgcg agcgcatcac gatcgactcg gtctcgaccc cggcaccggt aaggcgaacc
     7861 ccccgcaaca gcgcgccgct cgtcactccc gtcgccgaca cgaacacgtt ctcgcctcgc
     7921 acgagatcat cgtg
//
LOCUS       DBNGNK010000072         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1588459,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000072 DBNGNK010000000
VERSION     DBNGNK010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1588459"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..519)
                     /locus_tag="AC150V_01855"
     CDS             complement(184..519)
                     /locus_tag="AC150V_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3761698.1"
                     /translation="MLTVVVLVLSLIFLPSPWKFVVPVVFAILDLAETGFVLWWSQRG
                     KTQVGIETLVGRNAVAVGALRPSGKVKIDGELWEARSVTPVDPGSEVLIERVDGLVLD
                     VSPSSASAE"
     gene            complement(641..1027)
                     /gene="rplQ"
                     /locus_tag="AC150V_01860"
     CDS             complement(641..1027)
                     /gene="rplQ"
                     /locus_tag="AC150V_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009849468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR3761699.1"
                     /translation="MRHKRAGKKLGRDSAHRKALYSNLAGALITHGRIQTTEAKAKAV
                     KPFAEKMITLGKRGDLHARRQAMAALRSNDVVHLLFHEVAPRFEERPGGYTRIVKLGP
                     RQGDAADMAYLELVDYDPQASFAAGA"
     gene            complement(1029..>1659)
                     /gene="rpoA"
                     /locus_tag="AC150V_01865"
     CDS             complement(1029..>1659)
                     /gene="rpoA"
                     /locus_tag="AC150V_01865"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR3761700.1"
                     /translation="DIEGPADLEILNPDLEIANISDKSKLEMTLTVGRGRGYVPAELN
                     RGPETTIGVIPIDSIFSPVRRVAYEVEAARVGQRTDYDKLRLDLTTDGSFEPREAIGA
                     AAEILIRQLAIFTDIDKIEGFGEAPVDEEAEATVSHGMENFPIEELELGVRSYNCLKR
                     VGIETIGDLTSKTEAELAAIPNFGRKSIEEVRETLGAHGLTLRGEELVG"
BASE COUNT          263 a          562 c          556 g          278 t
ORIGIN      
        1 cggtcgcatg gacgcccgcg tcggtccgcc ctgcgacggc aagaccgtcc cagcggggaa
       61 agatctcgcc gagcgcctct cgaagcactc cctcgaccgt gcggcggcgc ggctggcgcg
      121 cccacccgtc gaaagacgta ccgtcgtact cgagcgtcag cttgagaatc attcggcttc
      181 cggttactcg gcggaggcgg aggaggggga gacgtcgagc accaagccgt cgacccgctc
      241 gatcaacacc tcgctccccg gatcgaccgg cgtcacgcta cgtgcctccc agagctcgcc
      301 gtcgatcttg accttgccgc tgggccgaag cgcaccgacg gcaaccgcat tgcgaccgac
      361 cagcgtctcg atgcccacct gcgtcttgcc acgctgcgac caccagagga cgaacccggt
      421 ctccgccagg tcgagaatcg cgaacacgac cggcacgacg aacttccacg gcgacggcag
      481 gaagatcaac gagaggacga ggacgacgac ggtgagcatc gctccctcga gcgtactcgg
      541 cgcacggtcc gggcgacggc tactcgcagg cggtcctgcg aggccgcagc atggtgctgc
      601 gagtggcgtc agccggacgc ggagggcggc tcggccggcg tcaggcgccg gcggcgaacg
      661 atgcctgggg gtcgtagtcg acgagctcga ggtaagccat gtcggcggcg tcaccctgac
      721 gcggcccgag ctttacgatc cgcgtatagc cgcccggtcg ctcctcgaag cggggagcga
      781 cctcgtggaa gagaagatgc acgacgtcgt tcgaccgcag ggcggccatc gcctgccggc
      841 gtgcgtgcag gtcgccacgc ttaccgagcg tgatcatctt ctctgcgaac ggcttgacgg
      901 ccttcgcctt ggcctcggtc gtctggatcc gcccgtgcgt gatcagcgct ccggcgaggt
      961 tcgagtagag cgccttgcga tgggcggagt cccgtcccag cttcttgccg gcacgcttgt
     1021 ggcgcatctc agccgacgag ctcctcgccg cgaagcgtga gaccgtgcgc accgagggtt
     1081 tcgcggacct cctcgatcga cttgcgcccg aagttgggga tggccgccag ctcggcctcg
     1141 gtcttcgagg tcaggtcacc gatcgtctcg atgccgacgc gcttgaggca gttgtacgag
     1201 cggacgccga gctccagctc ctcgatcggg aagttctcca tcccgtgcga gacagtcgcc
     1261 tcggcctcct cgtcgacagg tgcttcgcca aacccttcga tcttgtcgat gtccgtgaag
     1321 atcgcgagct gccggatgag gatctcggcg gccgcgccga tcgcctcgcg cggctcgaac
     1381 gatccgtccg tcgtcaggtc gagtcggagc ttgtcgtaat cggtgcgctg tccgacgcgg
     1441 gcggcctcga cctcgtaggc gacgcgacga acaggcgaga agatcgagtc gatcgggatg
     1501 accccgatag tcgtctccgg cccacggttc agctcggcgg gcacgtagcc tcggccacgg
     1561 ccgaccgtga gcgtcatctc gagcttgctc ttgtcgctga tgttggcgat ctcgagatcg
     1621 gggttcagga tctcgagatc tgccggtccc tcgatgtcg
//
LOCUS       DBNGNK010000073         2265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1301875,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000073 DBNGNK010000000
VERSION     DBNGNK010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1301875"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..964
                     /locus_tag="AC150V_01870"
     CDS             53..964
                     /locus_tag="AC150V_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3761701.1"
                     /translation="MRYVVTGAAGFIGSNLAHALERDGHTVLGLDCFTDYYDLSIKEG
                     NAAGLDVVRVDLAADELDFRGHDGVFHLAGQPGVRSFGDVFDLYLRRNVLASQRVFEA
                     AARDGVRVVFSSSSSVYGETDVYPTPEDAPLRPLSPYGITKLTCEHLARAYERSFGLD
                     VVVLRYFNAFGPGQRPDMAFTRIAFALAEEKVFNLFGDGLQSRSFTYVDDIVAATVLA
                     MERGSGTLNVGGGTEATIRDAIGILEEASGRRLDVSTHPAVPGDQRRTQADTSRIRSE
                     LGWRPHVSLEDGLSAQWEWAAGRVAPR"
     gene            961..1863
                     /locus_tag="AC150V_01875"
     CDS             961..1863
                     /locus_tag="AC150V_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR3761702.1"
                     /translation="MSQGEHPVPELDAEQEVDLRSAWQRIAHRWWLPVGGVIVGVLVG
                     IVVSVGGGDVFEAKTLVYLGQPYTPGGGSQIGNLNTNVSTVNEIVRSEAALKQAADAS
                     GLRIGQLRGNVKSDPIEAPGAVRGVSPIVEITVQAPQRAKAEKAADSLSGEVIDAVSE
                     YVADKVEILERSFEENKEEIAAADQRIQGALRQSQLAVDSPDLSLAERLLIQANANNT
                     LQFYEARQSNLRSNLNSLEQLLSLARNVEESRIVEPAVAKKTVATSRRNAALIGGLVG
                     LIAGAIAAYAWEPIARRRREQAAS"
     gene            1877..>2265
                     /locus_tag="AC150V_01880"
     CDS             1877..>2265
                     /locus_tag="AC150V_01880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3761703.1"
                     /translation="MVESKRVAVVVPAFAEEKLVAETLRGMPDFVDRVYVVDDASPDG
                     TAEQARTVSDPRVDVIVRETNGGVGAAIVTGYRRALEDGADVVCVMAADNQMDPSELI
                     ALVEPVARGEVDYAKANRLVTGEAWGT"
BASE COUNT          360 a          758 c          812 g          335 t
ORIGIN      
        1 gcggctctac ggcctcgcgc cccgcggctg agcgtccact aatctgccgc aaatgcggta
       61 tgtcgtcacc ggcgcagccg gcttcatcgg gtcgaatctg gcccacgcgc tggagcgcga
      121 cggtcacacc gtgcttggtc tggactgctt cacggactac tacgacctgt cgatcaagga
      181 gggcaacgcc gcggggctcg acgtcgtccg ggtcgacctg gcggccgacg agctcgactt
      241 ccgcggccac gacggggtct tccacctcgc gggccagccg ggcgtacgca gcttcggaga
      301 cgtgttcgac ctctacctcc ggcgcaacgt gctcgcctcg cagcgtgtct tcgaggccgc
      361 cgcgcgcgac ggggttcgcg tcgtcttctc gtcgtcctcg tccgtgtacg gggagacgga
      421 cgtgtatccg acgccggaag acgcgccgct gcgcccgctc tccccctacg gcatcacgaa
      481 gctgacgtgc gagcacctcg cccgcgccta cgagcggagc ttcggcctcg acgtggtcgt
      541 gcttcgctac ttcaatgcgt tcggtcccgg gcagcgcccc gacatggcgt tcacgcggat
      601 cgccttcgcg ctggccgagg agaaggtctt caacctcttc ggggacggcc tccagtcgcg
      661 cagcttcacg tacgtcgacg acatcgtcgc ggcgacggta ctggcgatgg agcgcggcag
      721 cggcacgctg aacgtcggcg gcggcacgga ggcgacgatt cgcgatgcga tcggaatcct
      781 ggaagaggcg tccggccgaa gactggacgt gagcacgcat ccggccgtcc cgggcgacca
      841 gcggcggaca caagcggata cctcgagaat ccggagcgag ctgggctggc ggccgcacgt
      901 ctcgctcgaa gacggactca gtgcccagtg ggagtgggcc gctggtaggg tcgcgcctcg
      961 gtgagtcagg gcgagcaccc agttcccgag ctcgacgcgg agcaggaagt cgatctccgc
     1021 tccgcctggc agcgcatcgc gcaccgctgg tggctgcccg tgggcggagt gatcgtcggc
     1081 gtgctcgtcg gaatcgtggt ctccgtcggc ggcggtgacg tcttcgaggc gaagacgctc
     1141 gtctacctcg ggcagccgta cacgcccggc ggcggatcac agatcggaaa cctcaacacg
     1201 aacgtcagca ccgtgaacga gatcgtccgc tccgaggccg cgttgaagca ggcggccgac
     1261 gcgagcggcc tgcgcatcgg gcagctgcgg ggcaacgtga agtccgaccc gatcgaggct
     1321 ccgggggcgg tccgcggcgt ttcgccgatc gtcgagatca ccgtgcaggc gccgcaacgc
     1381 gccaaggcgg agaaggccgc cgactccctc agcggcgagg tcatcgacgc ggtctcggag
     1441 tacgtggccg acaaggtcga gatcctcgag cggtcgttcg aggagaacaa ggaagagatc
     1501 gcggccgccg accagcggat ccagggcgcg ctgcgccagt cgcagctggc ggtcgacagc
     1561 cccgacctgt cactcgctga gcgcctgctg atccaggcga acgcgaacaa cacgctccag
     1621 ttctacgagg cgcgccagtc gaacctgcgc tcgaacctga actcgctcga gcagctcctc
     1681 tcgctggccc gtaacgtcga ggagagccgc atcgtggagc ccgcggtcgc aaagaagacc
     1741 gtcgcgacga gccggcgtaa cgcggccttg atcggaggac tggtcggcct catcgccggc
     1801 gcgatcgccg cctacgcgtg ggagccgatc gcgcgccggc gccgcgagca ggccgcgtcc
     1861 tagcgcgagg acgtcggtgg tcgagagcaa gcgcgtcgcg gtcgtggtgc cggccttcgc
     1921 cgaggagaag ctggtcgcag agacgctgcg cgggatgccc gacttcgtcg accgcgtcta
     1981 cgtcgtcgac gacgcttcgc ccgacggaac tgcggagcag gcgcgcacgg tttccgaccc
     2041 gcgggtggac gtgatcgtgc gggagacgaa cggtggtgtc ggggcggcga tcgtgaccgg
     2101 gtaccggcgg gctctcgaag acggcgccga cgtcgtgtgc gtcatggcgg ccgacaacca
     2161 gatggatccg tcggagctga tcgcgctcgt cgagcctgtc gctcgcggcg aggtcgacta
     2221 cgcgaaggcg aacaggctgg tcaccggcga ggcctgggga acgat
//
LOCUS       DBNGNK010000074         2841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1731967,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000074 DBNGNK010000000
VERSION     DBNGNK010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1731967"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1656
                     /gene="aceB"
                     /locus_tag="AC150V_01885"
     CDS             169..1656
                     /gene="aceB"
                     /locus_tag="AC150V_01885"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR3761704.1"
                     /translation="METADVLTPEATEFLVDLQREFEERRLELLAHRGERLERLRAGE
                     LPDFLPETESVRAGDWRIAPFPDEIADRRVEITGPVERKMVINALNSGARVFMADFED
                     ANSPTWENCIQGQRNLTDALDRTIELVTGDKEYRLGDDPAVLFVRPRGWHLVERHFEV
                     DDAPISGSLFDFGLYFLRNHHRNGRFFYLPKLESHLEARLWNDAFVWAQDRLGVAPGT
                     IKATVLIETILAAFEMDEILYELRDHSAGLNAGRWDYIFSVIKKLGHRPEFVLPDRAD
                     VTMAVPFMRSYCELLVKTCHRRGAHAMGGMAAFIPSRRDPEVNAVALEKVTEDKEREA
                     SQGFDGTWVAHPDLVPVALAAFDAVLGDRPNQIDRQRDDVEVGPAELLDVAATPGEIT
                     ETGLRNNVSVGVQYLAAWLSGNGAAAINNLMEDAATAEISRSQIWQWLHHGHISAEDV
                     MRVTEDEVAALGPGYEDAREIFEEIATQPEYVEFLTVPAYARLAD"
     gene            1656..2339
                     /locus_tag="AC150V_01890"
     CDS             1656..2339
                     /locus_tag="AC150V_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3761705.1"
                     /translation="MPRAVLIGGSSGIGLATAERALAAGWDVVIASRNPGRAEIDAEK
                     VELDVTDHDAVRALFGRLGTIDHLVSSTVARASGPVRELDLGAARHAFDVKLWGPLAA
                     VQAADVRESIVLVSGILAHAPSAGGAATAAVNGAVEALTRTLAVELAPVRVNAVCPGF
                     VDTPTWAGMPEPDRRALFDKMAETLPSGRVGRADDLADGIWHLLTNGFVTGTVLHVDG
                     GHRLSPPPR"
     gene            2336..>2841
                     /locus_tag="AC150V_01895"
     CDS             2336..>2841
                     /locus_tag="AC150V_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761706.1"
                     /translation="MIVRRSADPVAFTERAGTFLATSEAEHNLALGLLGRLRDEPQPY
                     GHDPWFAVAEDAGLVVGCALRTPPHGVVLCRCERPAAAAAIAEAARDSFDSLPGLVGP
                     SEAASRFAQTWQRLTGAGGRVVSRQRIHACTAVEALPEVDGRIRSAAADHRETIVAWI
                     AAFAAEALP"
BASE COUNT          445 a          991 c          983 g          422 t
ORIGIN      
        1 acgacgacgg cgtcgtcgtc gtgcctgccg cgatcgccgg agaggtgctc gcggacgccg
       61 agatgatggc gacgacggag aacaagatcc gctcggccgt ccgcgacgga atgacgccgc
      121 tcgaggccta cgagcgctac ggaaccttct agcccgacaa ggaggaacat ggagacagcg
      181 gacgtcctga cccccgaggc gacggagttc ctcgtcgatc tgcaacggga gttcgaggag
      241 cggcggctgg agctgctcgc ccaccgcggc gagcgactcg agcgactgcg ggccggggag
      301 ctgcccgact tccttcccga gaccgaatcg gtgcgggcgg gcgactggcg aatcgcgccc
      361 ttccccgacg agatcgccga ccggcgcgtg gagatcaccg ggcccgtcga acggaagatg
      421 gtgatcaacg cgctcaattc cggcgctcgg gtcttcatgg ccgacttcga ggacgcgaac
      481 tcaccgacct gggagaactg catccaaggt cagcggaacc tgaccgatgc gctcgaccgc
      541 acgatcgagc tcgtgacggg cgacaaggag tacaggctcg gggacgaccc ggccgtcttg
      601 ttcgtccggc cgcggggctg gcatctcgtc gagcgccact tcgaggtcga cgatgccccg
      661 atctcgggca gcctgttcga cttcggcctg tacttcctcc gtaaccacca ccgcaacgga
      721 cgcttcttct acctgccgaa gctcgagtcg cacctcgagg ccaggctctg gaacgacgcg
      781 ttcgtatggg cgcaggaccg gctcggcgtt gcgccgggga cgatcaaggc gactgtcctg
      841 atcgagacca tcctcgccgc attcgagatg gacgagatcc tctacgagct gcgtgaccat
      901 tcggccggac tcaacgcggg acgctgggac tacatcttca gcgtgatcaa gaagctcggc
      961 catcgccccg aattcgtcct gcccgaccgc gctgacgtga cgatggccgt tcctttcatg
     1021 cgctcctact gcgagctgct cgtcaagacc tgccaccgtc gcggcgctca tgcgatgggc
     1081 gggatggccg ccttcatccc ctcgcgtcgc gaccccgagg tcaacgccgt cgcgctcgag
     1141 aaagtgaccg aggacaaaga aagggaggca tcccaggggt tcgacggaac gtgggtcgcc
     1201 catcccgacc tcgtgccggt ggcgcttgca gccttcgacg ccgtactcgg cgaccggccc
     1261 aatcagatcg accgacagcg agacgacgtc gaggtcggcc ccgccgagct gctcgacgtc
     1321 gccgcgaccc cgggcgagat caccgagacg gggctgcgca acaacgtctc cgtcggtgtc
     1381 cagtacctcg cggcctggct gagcgggaac ggcgcggccg cgatcaacaa cctgatggag
     1441 gacgctgcga cggcggagat ctcgcgcagc cagatctggc agtggctgca ccacggtcac
     1501 atctcggccg aggacgtaat gcgcgtcacg gaggacgagg tcgcggcgct agggcccggt
     1561 tacgaggatg cccgggagat cttcgaggag atcgcgacac agccggagta cgtcgagttc
     1621 ctcaccgtgc ccgcctacgc gaggctggcc gattgatgcc gcgggcggtg ctgatcggcg
     1681 gctcgagcgg gatcgggctc gcgacggccg agcgcgcgct cgcagccggc tgggacgtcg
     1741 tgatcgcctc tcgcaacccc ggccgtgccg agatcgacgc cgagaaggtg gagctcgacg
     1801 tcacggatca cgacgccgtc cgggctctct tcgggcggct cggcacgatc gaccacctcg
     1861 tctcctccac tgtcgcccgc gcgtccggtc cggtgcggga gctcgacctc ggcgcggccc
     1921 gccacgcatt cgacgtgaag ctctgggggc cgctcgccgc ggtgcaggca gcggacgtgc
     1981 gcgagtcgat cgtgctcgtc tccgggatcc tcgcgcacgc tccgtcggcg ggcggagcgg
     2041 cgaccgcagc cgtcaacggc gccgtcgagg cgctcacgcg cacgctggcc gtcgagctcg
     2101 ccccggtgcg tgtgaacgcc gtctgtcccg gtttcgtgga caccccgaca tgggcgggta
     2161 tgcccgaacc ggatcgcaga gcgttgttcg acaagatggc cgagacgttg ccgtcagggc
     2221 gggtgggtcg cgcggatgat ctcgccgacg gaatctggca cctgctgacg aacggcttcg
     2281 tcacgggaac cgtgctgcac gtcgacggcg gccatcgtct gagcccgccg ccgaggtgat
     2341 cgtccgccga tcggccgacc ctgtcgcgtt caccgaacgc gccgggacgt ttctcgccac
     2401 cagcgaggcc gagcacaacc tggcgctcgg tctgctcggg cgcctgcgcg acgagccgca
     2461 accgtacggc cacgacccct ggttcgccgt cgccgaagac gccgggctgg tcgtcggctg
     2521 tgccctccgc actccccccc acggcgtcgt gctctgtcgg tgcgagcggc cggcggccgc
     2581 cgccgcaatc gccgaggccg cccgcgacag cttcgactcg ctgcccggat tggtgggccc
     2641 ctccgaggcg gcgagccgct tcgcccagac ctggcaacgc cttaccggag ccgggggacg
     2701 cgtcgtgtcg cgccagcgga tccatgcgtg caccgcggtc gaggcgctcc ccgaggtgga
     2761 cggacggata cggtccgcgg cggccgacca ccgggagacg atcgtggcct ggatcgcggc
     2821 gtttgcagcg gaggcgctcc c
//
LOCUS       DBNGNK010000075         3169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_513631,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000075 DBNGNK010000000
VERSION     DBNGNK010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_513631"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..285)
                     /locus_tag="AC150V_01900"
                     /pseudo
     CDS             complement(1..285)
                     /locus_tag="AC150V_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
     gene            complement(298..603)
                     /locus_tag="AC150V_01905"
     CDS             complement(298..603)
                     /locus_tag="AC150V_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761707.1"
                     /translation="MKRTSTVRTALSYVAAFVLLGAATTLAQTTDQELRDEIEALKKG
                     QEQIQKDLAEIKKLIQTRAPARPAVPNVKDKVFELRDNPVKGEQTAKLTLVEFTDYQ"
     gene            complement(732..1358)
                     /gene="hisIE"
                     /locus_tag="AC150V_01910"
     CDS             complement(732..1358)
                     /gene="hisIE"
                     /locus_tag="AC150V_01910"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020851328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR3761708.1"
                     /translation="MSAVTEGLDWAKGDGLLPAVVQDADTGAVLMLAYMSPESLEETE
                     ASGLVTFVSRSRQALWTKGETSGNQLELVEIRTDCDRDTLLVRARPSGPVCHLGSANC
                     FETGDEPGGFVPVLERIIAARAEASPEESYTATLFEAGVQRIAQKVGEEAVEVALAST
                     QDDDDRLVDEVADLLYHLLVLLRARGLGFDDVEKRLRSRHRTAPPSDG"
     gene            complement(1355..2122)
                     /gene="hisF"
                     /locus_tag="AC150V_01915"
     CDS             complement(1355..2122)
                     /gene="hisF"
                     /locus_tag="AC150V_01915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018973696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3761709.1"
                     /translation="MGARRIIPCLDVRGGEVVKGIRFRNHRVMGEIVDLAKRYRNDHA
                     DELVFYDIVASPEGRRVDKRWVEDVAAELDIPFCVAGGIRSVADAEETLAAGADKISV
                     NSPALERPELIDELAARFGTQCVVVGVDSRWEEGEWYVFQYTGDEERTLSTQRRTLAW
                     IVEVQERGAGEIVLNCMAHDGTREGYDLAQLEAARDVCSVPLVASGGAGSGEHIREVF
                     ERTEVDAALAATIFHTNEVRIPRLKSMLLDAGIEVRP"
     gene            complement(2104..2841)
                     /locus_tag="AC150V_01920"
     CDS             complement(2104..2841)
                     /locus_tag="AC150V_01920"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019399714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3761710.1"
                     /translation="MHVIPSIDIQGGRCVRLRQGRFDDVTEYDLDPLERALEYAAAGF
                     RRVHLVDLDGCREGKPRNRPILASIAAADGLEVQAGGGIRDRAAADGYLAAGVGRVIV
                     GSVAFREPARVLAWLGELGADRLVLAADVRTDGGAPRPVIDGWQREAAVDLDAFLDRF
                     VAAGLRHLLCTDVGRDGMQEGPNHTLYAELCRRHPGVAVQASGGVRDTRDLARLEEAG
                     ADAAIVGRSLLDGTLELEEIERWAPVE"
     gene            complement(2843..>3169)
                     /gene="hisH"
                     /locus_tag="AC150V_01925"
     CDS             complement(2843..>3169)
                     /gene="hisH"
                     /locus_tag="AC150V_01925"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3761711.1"
                     /translation="DAETCLGVVPGRAHRLEAARRRPVPHMGWSKVEPTSPSRLLEGI
                     PQGTYFYFVHSYAVPVLESTTAVTSYGSTFSSVVEHGRFFGTQFHPERSSIQGARVLH
                     NFLNLS"
BASE COUNT          483 a         1073 c         1102 g          511 t
ORIGIN      
        1 cgcgtcggca tgcttgccag tgctcatgca ggcctcgaag acggccacat cgagctccag
       61 cgcttcggcg tgggcgctcc agggctcgag agtgcgctgg ttctggaaga ggcgttcgta
      121 catctcccag aacttgccct gatcctctgc gcagtgtgag gcttctgccg ccttgaaggc
      181 cagcttgtgg atactctcca gcggcaggtc gagcggcgcg tagcggagct tgccggtctc
      241 gatgtactcc ttggagatct gcgggtacgt ctcacggacg tgcctggcgc agtacggtca
      301 ctgatagtcg gtgaactcga ccagcgtgag cttggccgtc tgctccccct tgactgggtt
      361 gtcgcgcagc tcgaacacct tgtccttgac gttcggcacg gccggccggg ccggagctcg
      421 ggtctggatg agcttcttga tctcggcgag gtccttctgg atctgctcct ggcccttctt
      481 gagcgcctcg atctcgtccc tgagctcctg gtcggtggtc tgagcgaggg tcgtcgcagc
      541 gcccagcaac acgaatgctg ccacatagga gagcgctgtc cgaacagtgc tagtccgctt
      601 cattccggtt ccggtctctg tctggaagac aacactaaaa acctatccca ctcgaggggc
      661 cgcgtcaacc tacatgcggt cgcgaatcag ccgtcatggc catcgagact cggcgaaaga
      721 tcgagccggg ctcagccgtc ggacggcgga gcggtccgat gccgtgagcg caggcgtttc
      781 tccacgtcgt cgaagcccag cccccgcgca cggagcagca cgagcaggtg gtagaggaga
      841 tcggcgacct cgtcgacgag acgatcgtcg tcgtcctggg tcgaagccag ggcgacttcg
      901 accgcttcct cgcccacctt ctgggcgatg cgctggacgc cggcctcgaa gagcgtcgcg
      961 gtgtagctct cctcgggcga cgcttcggcc cgcgccgcga tgatgcgctc gagaaccggg
     1021 acgaaaccgc ccggctcgtc gccggtctcg aagcagttgg cgctcccgag gtggcacacc
     1081 ggcccgctcg ggcgggcgcg caccagcagg gtgtcgcgat cgcagtcggt gcggatctcg
     1141 accagctcga gctggttgcc cgacgtctcg cctttggtcc agagagcctg ccgcgagcgg
     1201 ctgacgaagg tgacaagacc gctcgcctcg gtctcctcga gcgactccgg cgacatgtag
     1261 gcgagcatca gcacggcgcc ggtgtcggcg tcctgcacga cggccggcag cagaccgtcg
     1321 cccttggccc aatcgagccc ctcggtcacg gcgctcacgg tcgcacctcg atcccggcgt
     1381 cgagcagcat cgacttgagc cggggaatgc ggacctcgtt ggtgtggaag atggtcgcgg
     1441 ccagggcggc gtcgacctcg gtgcgctcga agacctcgcg gatgtgctcg ccgctgccgg
     1501 cgcctccgga ggcgaccagc ggcaccgagc agacgtcccg ggccgcctcg agctgcgcga
     1561 gatcgtagcc ctcgcgggtg ccgtcgtgag ccatgcagtt gagcacgatc tctcccgcac
     1621 ctcgctcctg aacctcgacg atccacgcga gcgtccgtcg ctgcgtcgag agcgtgcgct
     1681 cctcgtcgcc ggtgtactgg aagacgtacc actcgccctc ctcccaacgg ctgtcgacgc
     1741 cgacgaccac gcactgcgtc ccgaagcggg cggcgagctc gtcgatcagc tccggcctct
     1801 cgagagcggg cgagttgacc gagatcttgt cggcacccgc ggccagggtc tcctcggcgt
     1861 cggcgacgct gcggatgccg ccggcgacgc agaagggaat atcgagctcg gcggcgacgt
     1921 cctcgaccca gcgcttgtcg acacgcctac cctcgggaga cgcgacgatg tcgtagaaga
     1981 ccagctcgtc ggcgtggtcg ttgcggtagc gcttggcgag gtcgacgatc tcgcccatca
     2041 cccgatgatt gcggaagcgg atgcccttga cgacctcgcc gccgcggacg tcgagacagg
     2101 ggattattcg acgggcgccc atcgttcgat ctcctcgagc tcgagcgtgc cgtcgaggag
     2161 cgaccgcccg acgatcgcgg cgtcggcgcc cgcctcttcc agtcgggcga ggtcgcgcgt
     2221 gtcacggacg ccacccgacg cctgcacggc cactccgggg tggcgccggc agagctcggc
     2281 gtagagcgta tggttggggc cctcctgcat gccgtcgcgc ccgacgtcgg tgcacagcag
     2341 atggcgcagg cccgcggcga cgaagcggtc gaggaaggcg tcgagatcga cagcggcctc
     2401 gcgctgccag ccgtcgataa ccggccgcgg agccccgcca tcggtgcgga cgtccgccgc
     2461 caggaccagc cggtcggcgc cgagctcccc gagccacgcc agcacgcggg caggctcgcg
     2521 aaaggccacg ctgccgacga tgacgcgccc gacgccggcg gcgaggtagc cgtcggcggc
     2581 ggcgcggtcg cggatgccgc ctccggcctg gacctcgagg ccatcggcgg cggcgatcga
     2641 ggcgaggatc gggcggttgc gtggcttccc ttcgcggcag ccgtcgagat cgaccaggtg
     2701 gacccggcga aagccggccg cggcgtactc gagcgcccgc tccagagggt cgaggtcgta
     2761 ctcggtgacg tcgtcgaagc ggccttgccg caatcggacg cagcgaccac cctggatgtc
     2821 gatcgacggg atgacgtgca tctcaggaga ggttgagaaa gttgtgcagg accctggcgc
     2881 cctgaatcga ggagcgctcg gggtgaaact gcgtgccgaa gaatctgccg tgctcgacga
     2941 cgctcgagaa ggtcgagccg tagctcgtca ctgcggtggt cgattcgagc accggtaccg
     3001 cgtagctgtg gacgaagtag aagtaggtcc cctgcgggat gccctcgagc agccggctcg
     3061 gggaggtcgg ctcgaccttg ctccagccca tgtggggcac ggggcggcgc cttgccgcct
     3121 ccagccggtg ggcgcgcccg ggaacgacgc cgaggcacgt ctcggcgtc
//
LOCUS       DBNGNK010000076         5306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1732146,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000076 DBNGNK010000000
VERSION     DBNGNK010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1732146"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="AC150V_01930"
     CDS             <1..1174
                     /locus_tag="AC150V_01930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR3761712.1"
                     /translation="AGFAGLAAADMLVRAGLDVVVLEARDRVGGRVWSRQLDNGAVIE
                     LGAEFILPGNDTILAFAERLGLGLWDKGMRYGVREPRGGVGVEPGQLEEGLAVIAMAL
                     GRGEGEGLTAREFLDRLRLDPGARDAIRSRLEISCAATADAVDANVLDGLAAHGDDPS
                     PSVAFGNQRIALALAGELGSAVHLSSPVEHITWDDIGARVSASGAELEADRVVLAMPA
                     SVVDRIVFEPPLSARLARAYASVEYGHAAKLFVPLSEPAFPSAVLSVPERYWTWTATG
                     EGAVQPVAHAFAGSSPALAGLEVEQGPETWAASIARLRPELRLDLAGTVLSTWDDDPW
                     VAAAYSSRAPGEPAWTAAGPFHACGEHTAGERGGLMDGALASEIRAAAEVVGALGR"
     gene            complement(1190..2320)
                     /locus_tag="AC150V_01935"
     CDS             complement(1190..2320)
                     /locus_tag="AC150V_01935"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3761713.1"
                     /translation="MSTVRDILPDRAGGLPAPPRALADGWTLPASWYTDPAVLRLEEE
                     RIFARAWQYAGRAAEVEDVGCYLTARAGNVPIVVVRGRDGVLRGFVNVCRHRGHVVVQ
                     EAGCRETLQCPYHAWTYDLDGSLRSAPRADREPDFDPAGLSLLPASVGTWGPFVFVNP
                     DPAAPPLHDTLSDLPDVVAESGLDLDAVRFHSHLEAQFRGNWKVAMENFLECYHCAVA
                     HPGFSKVIDVDPDEYLLRVEPTYLSQVGHVRAAVLAGEREAPYDPRGEIEQAQYHVLF
                     PNTTINISPGFPNVAIESWLPVGTDQTLEVTDYFFAPDASEEQVAEFLAFDTQVANED
                     AALVASVQTGLESGMVPQGRLMGSSEQLIAAFQRRVMGALQV"
     gene            2394..3482
                     /locus_tag="AC150V_01940"
     CDS             2394..3482
                     /locus_tag="AC150V_01940"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761714.1"
                     /translation="MTAPNVRHGVVLDGVTKTFDEVVAVDDVSVVIEPAEFFSLLGPS
                     GCGKTTSLRMVAGFEKPDAGRVLIGETEVTDVPPHKRPVNTVFQSYALFPHLSVGENV
                     QFGLRFRKVSKSEKRRRAAEMLEVVRLQGYEDRKPHQLSGGQQQRVALARALILSPEV
                     LLLDEPLGALDGKLRRELQIELKGLQREVGITFVYVTHDQEEALTMSDRIAVMNEGRI
                     EQLGSPRRLYEEPATPFVANFLGVSNLMRASCDGTGNVLLEGGEHRAEAGETETVGDV
                     HVTIRPERVEVEARHDPGPNRFAGHVRSLVYLGSATQVILRLQSGTELQALAPSVGQP
                     LPFGEGDAVTVHLPPGAIRVIAGPEPPA"
     gene            3479..4792
                     /locus_tag="AC150V_01945"
     CDS             3479..4792
                     /locus_tag="AC150V_01945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin monoamine oxidase family protein"
                     /protein_id="HIR3761715.1"
                     /translation="MSDVVVVGAGLAGLSAAYDLAAGGADVVVLEARDRVGGRVEQVT
                     IDDGRPVQLGGEVVGRAHTAYLGLVEELGLTVQPSYVEAPGHTTYALLEGTERSDEAF
                     PFTGEEERDDYARVERLFGELVATVDPDDPWSHADASRLDSTSIGTWLRSVDALSTTV
                     RALTVGSLGLADGSIECSSLLAELRKAAAVGDRCFYDVERWESHQVTEGSAEVAIRLA
                     ERLGERVRLDAEVESIRVSSGGCRIRLADGEKLGAEAVVCALPVGVVRSIQIEGVRPE
                     RLESLHRQRNAVTAKIVVTFDRDVWADVDASGLADGEFLLGSTWPQRDRVLSALVPPE
                     RIGWLLAVSEQDRAEVVRDELERMYGSAARNTVETFVRLWGVDPYTLGYVTHWWPGDV
                     LRVGPLHGTHDPPFYVAGSDQWVAGYLEGAVQTGRAAAAAALGAR"
     gene            complement(4789..>5306)
                     /locus_tag="AC150V_01950"
     CDS             complement(4789..>5306)
                     /locus_tag="AC150V_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761716.1"
                     /translation="TPELVMGVALFLVIVNLFTFIPLGTTAQAIGHVTFSISFVLIIV
                     RGRLLSIGRDYEEASMDLGASPLNSIRMILLPLLMPAVLASAIIVFAISMDDFVISAY
                     LASGSATETVPVKIYSNARGSPTPALNALASLTLFLTIILAVVMFVAWSLVRRRQGGG
                     GSAVDEFARLD"
BASE COUNT          825 a         1788 c         1912 g          781 t
ORIGIN      
        1 ggcaggcttc gccggactcg ccgccgccga catgctcgtg cgagccggcc tcgacgtcgt
       61 cgtactggag gcccgcgacc gcgtcggcgg ccgggtctgg tcgcggcagc tcgacaacgg
      121 cgcggtcatc gagctgggag cagagttcat tctccccggg aacgacacga tcctggcctt
      181 cgcagagcgg ctgggactgg gcctctggga caaagggatg cgctacgggg tgcgagagcc
      241 ccgcggcggc gtcggcgtcg agccggggca gttggaggag ggcctcgctg tgatcgcgat
      301 ggcgctcggc cgcggcgaag gcgaaggtct gactgcgcgg gagttcctcg accgcctgcg
      361 gcttgatccc ggcgcccggg acgcgatcag atcgcggctc gagatctcct gcgccgcgac
      421 agcggacgcc gtggacgcga acgtgctcga cggacttgcc gcgcacggcg acgacccctc
      481 gccgagcgtc gcgttcggca accagcgaat cgccctcgcg ctggccggcg agctcggctc
      541 ggccgtccac ctgtcgagcc ccgttgagca catcacctgg gacgacatcg gcgcccgggt
      601 ctccgcctcc ggcgccgagc tcgaggccga tcgagtcgtg ctcgccatgc cggcaagtgt
      661 cgtcgaccgc atcgtgttcg agccgccgct ctccgctcgg cttgcgcgcg cgtacgcatc
      721 ggtcgagtac gggcacgcgg cgaagctgtt cgttccgctc tccgagcccg cgttccccag
      781 tgccgtcctg tccgtgccgg agcgctactg gacgtggacg gcgaccggag aaggcgccgt
      841 ccagcccgtc gctcacgcgt tcgcgggctc ctctcccgcc ctggcgggcc tcgaggtcga
      901 gcaggggccc gagacctggg ccgcctcgat cgcccgcctt cggcccgagc ttcggctcga
      961 cctcgctgga acggtgctct cgacctggga cgacgacccc tgggttgcag cggcgtactc
     1021 gagcagagca ccaggcgagc cggcctggac ggcggccggg ccgttccatg cctgcggcga
     1081 gcacaccgcg ggtgagcggg gcgggttgat ggacggtgcg ctcgcaagcg agatcagagc
     1141 agccgccgag gtggtcggcg ctctcggccg ctgagcggcg ccggactccc tacacctgca
     1201 gcgcgcccat gacccggcgc tggaacgcgg cgatcagctg ctcgctcgag cccatcagcc
     1261 gcccctgggg caccatcccg gactcgaggc cggtctggac ggaggcgacg agcgcggcgt
     1321 cctcgttcgc cacctgggtg tcgaaggcga ggaactccgc gacctgctcc tcggaggcgt
     1381 cgggagcgaa gaagtagtcc gtcacctcga gggtctgatc ggtgccgacg ggaagccagc
     1441 tctcgatcgc gacgttcggg aacccaggtg agatgttgat cgtggtgttc ggaaagagga
     1501 cgtggtactg ggcctgctcg atctcgccgc ggggatcgta cggagcctcc cgctcacccg
     1561 cgagcaccgc ggcgcggacg tgcccgacct ggctcagata ggtgggctcg actcgaagca
     1621 ggtactcgtc ggggtcgacg tcgatgacct tcgagaaccc gggatgcgcg accgcgcagt
     1681 ggtagcactc gaggaagttc tccatcgcga ccttccagtt cccccggaac tgcgcttcga
     1741 ggtgggagtg gaagcgcacg gcgtcgagat cgaggccgct ctcggccacg acgtcgggga
     1801 gatccgaaag cgtgtcgtgc agcggcggcg ccgccggatc ggggttgacg aagacgaacg
     1861 ggccccacgt cccgaccgat gccgggagca gcgagaggcc cgctggatcg aagtcaggct
     1921 cacgatcggc ccgcggcgcg ctgcggagcg agccgtcgag gtcgtacgtc caggcgtgat
     1981 acgggcattg cagcgtctcg cggcaacccg cctcctgcac gacgacgtga ccgcggtgcc
     2041 gacacacgtt gacgaagccg cgcagcactc cgtcccgacc ccgaaccacg acgatcggga
     2101 cgtttccggc ccgagcggtc aggtagcagc ccacatcttc cacctctgcc gcgcggcccg
     2161 cgtactgcca cgctcgggca aagatccgct cttcctcgag ccgcaggacg gccggatccg
     2221 tgtaccagct cgcgggaagc gtccagccgt ccgcgagcgc ccgcggaggc gccggcagac
     2281 cgccggctcg gtcggggagg atgtcgcgaa ctgtgctcat ttcggaggtt ggggcgtccc
     2341 gcccatcagg atacgctgca cgccaccatg gacgccaaga acggcgaaac ccggtgacgg
     2401 cgcccaacgt caggcacggt gtcgtcctcg acggcgtgac gaagacgttc gacgaggtcg
     2461 tcgccgtcga cgacgtcagc gtcgtgatcg agccggccga gttcttctcc ctgctcggtc
     2521 cgtcgggctg cggaaagacc acctcgctga ggatggtcgc gggcttcgag aagcccgacg
     2581 cggggcgggt cttgatcggc gagacggagg tgaccgacgt gccgccgcac aagcggcccg
     2641 tcaacacggt cttccagagc tatgccctct tcccacacct ctcggtcggc gagaacgtcc
     2701 agttcgggct gcgctttcgc aaggtctcga agagcgagaa gcggaggcgc gccgccgaga
     2761 tgctggaggt ggtccgcctg caaggctacg aggatcggaa gccgcaccag ctctccggcg
     2821 gccagcagca acgggtcgcg ctcgcccggg cgctgatcct ctcccccgag gtgctcctcc
     2881 tcgacgagcc gctcggcgcg ctcgacggaa agctgcggcg ggagctccag atcgagctca
     2941 agggcctgca gcgcgaggtc ggcatcacgt tcgtctacgt cacgcacgac caggaggagg
     3001 cgctgacgat gtccgaccgg atcgccgtga tgaacgaggg aaggatcgaa cagctgggct
     3061 cgccacgacg actgtacgag gagcctgcga cgccgttcgt cgcgaacttc ctcggcgtgt
     3121 cgaacctcat gcgagcatcg tgcgacggaa cgggcaacgt cctcctcgag ggcggcgagc
     3181 accgcgccga ggccggcgag accgagacgg tcggtgacgt ccatgtgacg attcggccag
     3241 agcgggtcga ggtggaggcc cgtcacgacc cggggcccaa ccgcttcgcc gggcacgtgc
     3301 gaagcctcgt ctacctcggc tcggcgacgc aggtgatcct caggctgcag agtggcaccg
     3361 agctccaggc gctcgccccg agcgtcggtc agccgcttcc ctttggcgag ggtgacgccg
     3421 tcacggtgca cctgccgccc ggcgcgattc gcgtgatcgc gggcccggaa ccccccgcgt
     3481 gagcgacgtc gtcgtcgttg gcgcgggcct cgccggcctc tccgccgcgt acgacctcgc
     3541 cgccggagga gcggacgtgg tcgttctcga ggcgcgcgac cgagtcggag gtcgcgtcga
     3601 gcaggtgacg atcgacgacg gtcgccctgt tcagctcgga ggcgaggtgg ttggcagggc
     3661 acacaccgcg tacctcggac tggtcgagga gctcgggctg acggtccagc ccagctacgt
     3721 cgaggcaccg gggcacacta cctacgccct gctcgagggc acggagcgct cggacgaggc
     3781 gttccccttc acgggggagg aggaacgcga cgattacgca cgtgtcgagc ggctcttcgg
     3841 cgagctcgtc gcgacggtcg acccggacga cccgtggtcg catgcggatg cgagtcgtct
     3901 cgattccaca tcgatcggga cgtggttgcg ctccgtcgat gcgttgtcga cgaccgtccg
     3961 cgcgctgacc gtcggatcgc tcggtctggc ggacggctcg atcgagtgct cgtcccttct
     4021 cgccgagctg cgcaaggccg ccgccgtcgg agaccgttgc ttctacgacg tcgagcgctg
     4081 ggagtcacac caggtgaccg aggggagcgc cgaggtggcg atccgtctcg ccgaaaggct
     4141 cggcgagcgc gtgcgcctgg acgccgaagt ggaatcgatc cgcgtgtcgt ccggcggctg
     4201 ccggattcga ctcgccgacg gcgagaagct gggcgccgag gctgtggtct gcgcgcttcc
     4261 ggtgggtgtc gtccgcagca tccagatcga gggcgtgcga cccgagcggc tggagtcgct
     4321 gcaccgtcag cgaaatgcag tgacggcgaa gatcgtggtc acgttcgacc gggacgtgtg
     4381 ggccgacgtc gacgcgagcg ggctcgcaga cggcgagttc ctgctcggct cgacctggcc
     4441 ccagagagac cgtgttctct ccgccctggt gccgccggag cgaatcggct ggctgctcgc
     4501 cgtttcggag caggatcgcg cggaggtcgt ccgcgacgag ctggagcgca tgtacgggtc
     4561 ggcggcgcgc aacacggtcg agacgttcgt ccggctgtgg ggcgtcgatc cctacacgct
     4621 cgggtacgtg acgcactggt ggccgggcga cgtcctccgc gtcggcccgc tgcacggcac
     4681 gcacgacccg cccttctacg tggccggctc cgaccaatgg gttgccggct atctcgaagg
     4741 agcggtgcag accggccgag ctgcggcagc cgccgcactc ggcgctcgct agtcgaggcg
     4801 cgcgaactcg tcgacggcgg agcctcctcc gccctgccgg cgccggacga gcgaccaggc
     4861 gacgaacatg acgaccgcca ggatgatcgt caggaagagc gtcaggctcg cgagcgcatt
     4921 gagtgccggc gtgggcgacc cccgcgcgtt ggagtagatc ttcacgggaa cggtctcggt
     4981 cgcggagccc gacgcgaggt aggcgctgat gacgaaatcg tccatcgaga tcgcgaagac
     5041 aatgatcgcg ctcgcgagga ccgcgggcat gagcagcggc agcaggatca tgcggatcga
     5101 gttgaggggc gatgcaccga ggtccatcga cgcctcctcg tagtcccgcc cgatagataa
     5161 gagccgcccg cgcacgatga tcaacacgaa ggagatggag aacgtcacat gtccgatcgc
     5221 ctgggcggtg gtgccgagcg gaatgaacgt gaacaggttc acgatgacga ggaagagcgc
     5281 aacgcccatg acgagctccg gggtga
//
LOCUS       DBNGNK010000077         2514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1804050,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000077 DBNGNK010000000
VERSION     DBNGNK010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1804050"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC150V_01955"
     CDS             <1..395
                     /locus_tag="AC150V_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761717.1"
                     /translation="FRTWALWMLDSQLELALELDLLDEAEQTGLEGLELATLLNDYRL
                     TRALLAGLALVALRRSGVERAGGLRGAVSASEEEAPILSAFSTFASYTEPLRACSDDR
                     FLAGVEIGRLLTLESAVQLALGETPLPS"
     gene            405..>2514
                     /locus_tag="AC150V_01960"
     CDS             405..>2514
                     /locus_tag="AC150V_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR3761718.1"
                     /translation="MRLHRCGHRLDHTPFPDDSLDDSIMGRFARQVEQHRDCIAIRDG
                     NHSITYRELDSWSNGIAVALLEELGGRPEPVALLHRQGAASIAATLGALKVGKPYVPL
                     DPGDPESRLRQLTERVGARVILSDRENATRAAAIRRPLVLVDGLSSARPPGFRASPDD
                     IAYVYFTSGSTGRPKGVYDNHRNVLHNVLRYTNALEIAPSDRITLLQSPSFSGVVSSL
                     FCALLNGATSCTYQLRGDGIAGLAEWVAREEITIYHSVPSIFRTLVATGRAFPHVRVV
                     RLEGDRAAAVDVELFERHFAPGCVLANGLGTTETGLARQLLVRHGEPVGEGILPVGYP
                     VRDVEVVIVDESRAELPQGSAGEIGVRSDYLALGYWNEPDLTSRSFLPAADGDTRRLY
                     CTGDVGRLRDDGCLEYLGRLDSQLKVLGTRVDLAEVESELARLAGIADAVVTTVESRR
                     GEGRLLAYVVNDDGSEIDAESVRRALADRLPPHLIPSGFATLGALPTDANGKVDRGRL
                     PPIEVPAGTSDRRDGELVATLAAIWSEVLEVPVGPDDDFFQLGGDSLAAAEIQARVAD
                     STGVELPLGSLVHAPTVRAQAREVWLGRRDGARSALVRLREGQTPPFVLLHGITGNAL
                     HYARLASCITREVVGIELGDPDSDLTVGSLAKRHVATLRAAQPDGPYLLGGFCSGAVL
                     AREMACLLAADGEQIALVVVL"
BASE COUNT          375 a          881 c          839 g          419 t
ORIGIN      
        1 ggtttcggac gtgggcgctt tggatgctcg attcccagct cgagctcgcg ctcgaactcg
       61 acctcctcga cgaagccgag cagacgggcc tggaggggct cgagctggcg acgcttctca
      121 acgactaccg gctcacgcgc gccctgctcg ccgggctggc attggttgcc ctacgccgta
      181 gcggcgtcga gagagcgggt gggctgcggg gcgcggtgtc cgcgtcggag gaggaagcgc
      241 cgatcctgag tgccttcagc acgttcgcgt cgtacaccga accgctccgc gcgtgtagcg
      301 acgatcgctt cctcgcagga gtcgagatcg gccgcctcct gacgctcgag agcgctgtcc
      361 agctcgcact gggcgagacg ccgctcccct cctagcatga gcccgtgaga ctgcaccgct
      421 gcgggcaccg cctcgaccac acgccctttc ccgacgactc tctcgacgac tcgatcatgg
      481 ggcgcttcgc ccggcaggtc gagcaacacc gcgactgcat cgcgattcga gacggcaacc
      541 actcgatcac ctatcgcgag ctcgactcgt ggtccaacgg catcgccgtc gccctgctcg
      601 aagagctcgg cgggcgaccc gagccggtgg ccctcctcca ccgtcagggc gctgcgagca
      661 tcgccgcgac gctcggcgcg ctcaaggtcg gcaagccgta cgttccgctc gatccgggcg
      721 acccggaatc gcgactgagg cagttgacgg agagggtcgg cgcccgggtg attctctcgg
      781 atcgggagaa cgccacccga gccgcggcga tcagacgccc gctcgtgctc gtggacggcc
      841 tctcgtcggc gcggccgccc ggcttccgtg cctcgccgga cgatatcgcc tacgtctact
      901 tcacatcggg ctcgacgggc cgtcccaagg gcgtctacga caaccaccgc aacgtactgc
      961 acaacgtcct tcgctatacg aacgcgctcg agatcgcgcc gtcggaccgg atcacgttgc
     1021 ttcagtcccc gagcttcagc ggcgtggtgt cgagcctctt ctgcgccctc ctcaacgggg
     1081 cgacgtcctg cacctatcag ctacgcggtg acggcatcgc tggcctcgcg gagtgggtgg
     1141 cccgcgagga gatcacgatc taccactcgg taccgtcgat tttccgcacc ctcgtcgcca
     1201 ccggcagggc gtttccccac gtgcgggtcg tccgcctcga aggcgatcgc gctgcggcgg
     1261 tcgacgtcga gctgttcgag cgccacttcg cgccgggatg cgtcctcgcg aatgggctcg
     1321 gcacgaccga gaccggcctc gctcgccagc tgctcgtgcg ccacggcgag ccggtcggcg
     1381 aagggattct tccggtcggc tatccggtgc gagacgtcga ggtggtgatc gtcgacgaat
     1441 cccgtgccga gctgcctcag ggcagcgcag gggagatcgg cgtccgcagc gactacctgg
     1501 cgctcggcta ctggaacgag cccgacctca cgtcccggtc gtttctgccc gcggccgacg
     1561 gagacacgcg gcgtctctat tgcacgggcg acgtgggtcg tcttcgcgat gacggctgcc
     1621 tcgagtacct gggacggctc gactcccagt tgaaggtgct cggcacccgc gtcgacttgg
     1681 ccgaggtgga atcagaactc gcgcggctag cggggatcgc ggacgcagtc gtgaccaccg
     1741 tcgagagtcg acgaggtgag ggtcgcctgt tggcgtacgt cgtcaacgac gacgggtcag
     1801 agatcgacgc cgagtcggtg cggcgggcac tggcagatcg cctcccgccc cacttgattc
     1861 cttccggttt cgccacactc ggagccctcc cgacagacgc gaatggcaaa gtggatcgtg
     1921 gtcggctgcc cccgatcgaa gttcccgcgg gtacgagcga ccgccgggac ggcgagctcg
     1981 tcgccacact cgcggccatc tggtcggagg tgctggaggt acccgtcggt cccgacgacg
     2041 atttcttcca gctcggcggg gactcgctcg ccgccgcgga gatccaggcg cgggtcgccg
     2101 actcgaccgg ggtggagctg ccgctcggct cactcgtaca cgcaccgacg gttcgagcgc
     2161 aggcacgtga agtctggctc gggcgacgtg acggagcccg cagcgcactc gtacggctgc
     2221 gggaaggcca aacccccccg ttcgttctcc tccacggcat caccggaaac gcactgcact
     2281 atgctcgcct cgcctcctgc atcacgagag aggtggtcgg aatcgagctc ggcgaccccg
     2341 actccgacct gaccgtcggt tccctggcga agcggcatgt cgcgacgctc cgagcagctc
     2401 agccggacgg tccgtatcta ctcggcggct tctgctccgg cgcagtattg gcgcgcgaga
     2461 tggcgtgcct gctcgcggct gatggggagc agatcgcgct cgtcgtcgtg ctag
//
LOCUS       DBNGNK010000078         4426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_657489,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000078 DBNGNK010000000
VERSION     DBNGNK010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_657489"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..2164)
                     /locus_tag="AC150V_01965"
     CDS             complement(224..2164)
                     /locus_tag="AC150V_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HIR3761719.1"
                     /translation="MSIPERRSRHSKPRPVLLVGNPNVGKSVLFRNLTNRYVTVSNFP
                     GTTVDVVRARARGVEESFEIIDTPGLNDLAPRSEEARVTRQLLDEAPDATVVQVADAK
                     NLRRALLLTLQLGDLARPMVLVLNMTDELDQRGGRIDRERLEAILGVPVVSAVAITNR
                     GTEELRAALERAAVPRLNGEGATALDDEYQRNKLLLSRVNEILEQTYSITQPRHASLA
                     VRLGFWAMHPVKGLVFLALALAVVFWFVGLLGAGTLVDLLETGVFEQRVTPLAIRVAD
                     SLLPFPHVHETASAEATVALPLSPVHEVEIASWSREVSGVAYRLVGEPSSWQRAARFG
                     HDLLVGEYGAVTMALSYAFSIVLPIVVTFFLLFSVIEDSGYLPRMAIMVNRLFRAMGL
                     NGKAVLPMVLGLGCDTMATMTTRILETRKERIVTTMLLALAVPCSAQLGVLLAMMANL
                     SAAGALIWLVLMVGVLFLVGWLTAKVFGGETSEFILEIPPMRRPQLENVAIKTVSRVN
                     WYLKEVIPLFVIGTAVLFVLDWTGLLARIARLGEPLVSGWLGLPREMANAFLVGFMRR
                     DFGAVYILDAATGPEPLLSTAQILVAMVTITLFMPCFANFLMIAKEHGQRVAWSMLAF
                     IFPFAFLVGGLVHHVGRWLIGS"
     gene            complement(2319..3668)
                     /locus_tag="AC150V_01970"
     CDS             complement(2319..3668)
                     /locus_tag="AC150V_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3761720.1"
                     /translation="MSAPPTTVTPLATYRRETPYPFCPGCGHGNILDALNAALVELQR
                     DPEQIVIVSDIGCSGLSDQYFATNAFHGLHGRSIAYASGIKLARPELEVVVVMGDGGT
                     GIGGAHLINAARRNIGMTVLVFNNFNFGMTGGQHSTTTPAGAATSTTPLGNLERPLDV
                     CGTVAVNGAAYVYRGTSFDRDLGARIAEAIASPGFALLDIWELCTAYYVPRNKSSKKA
                     LFEMLDELDLAAGVIARREVEGYETAYRTAHAELRGRPVLAAEPIERRFAASLARPMR
                     LVGAGAAGGKVRSAARLAGRAAILSGLWAAQREDYPVTVQTGHSISELILSPEPFAYT
                     GIEKPDATILLAAEGLGKARRYLSAMGPDDTVYVEASLPAVETAARVIALDLASAGSV
                     GRTGRSLALLTAALLDLEALSEESLMAAADNGPARHRDENRAAIAAGKALAQLSAGE"
     gene            complement(3665..>4426)
                     /locus_tag="AC150V_01975"
     CDS             complement(3665..>4426)
                     /locus_tag="AC150V_01975"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3761721.1"
                     /translation="QFARWGTAGGYPLIVLAPSSVLECFSLTRKAFDLAERFRCPVLL
                     LTDKELNMTAVTVDLDEYVEVPVRARARRSAERDTSGDFRPYRYEPAGDVPPIAHFGE
                     GDKVRFTGSTHDERAFITKDRATVEKLNRHLVEKIEAHRDEIDLVTSDLDGKARVLWI
                     SWGSTAGAMREAVSMARRNGRAVSALTIQSLWPVPEEEIVAAAEGADRIVVAELNHGQ
                     YRREVERLVGRERIVGLHRNDGELLAPSAFLETLT"
BASE COUNT          754 a         1522 c         1490 g          660 t
ORIGIN      
        1 cccggcgcca gctcgctgac cagctgcacc cgctcgcaga gctcgtctcc cggctggcct
       61 tcccgcccga ggagcctggc gagccaggag accgccgcgg gcttgctcgg gaactcgtag
      121 tcgcagctgg ggcagcgaat caagttgcag taggtccgca tcgggcagcc gtgctgacac
      181 agcgtgtccc cgggggcgaa ctcgaagccg cagagcggac agactatgaa ccgatcagcc
      241 atcgaccgac gtggtgcacg agacctccga ccaggaaggc gaagggaaag atgaaggcga
      301 gcatcgacca ggctacccgc tgcccgtgct ccttggcgat catcagaaag ttggcgaagc
      361 agggcatgaa cagcgtgatc gtcaccatcg caacgagaat ctgggcggtg ctgagcagcg
      421 gctcgggtcc ggtcgccgca tccaggatgt agaccgcgcc gaagtcgcga cgcatgaagc
      481 cgacgaggaa ggcgtttgcc atctcccgcg gcaaaccgag ccagccgctg accagaggct
      541 cgccgagccg cgcgatgcgg gccagaagtc ccgtccagtc gagcacgaac aggaccgcgg
      601 tgccgatcac gaacagcgga atgacctcct tcaagtacca gttgacccgg ctcacggtct
      661 tgatcgccac gttctcgagc tgtggcctgc gcatcggcgg gatctcgaga atgaactcgc
      721 tggtctcgcc gccgaagacc ttcgccgtca gccagccgac gagaaacagc actccgacca
      781 tcaacaccag ccagatcagc gccccggctg ctgagagatt ggccatcatc gccagcagca
      841 cgccgagctg tgccgagcac ggcaccgcga gcgccagcag catggtcgtg acgattctct
      901 ccttgcgcgt ctcgaggatt ctcgtggtca tcgtcgccat ggtgtcgcag ccgagaccga
      961 gcaccatcgg cagcaccgcc ttgccgttca atcccatggc ccggaacaga cgattgacca
     1021 tgatcgccat gcgcggcagg tacccggaat cctctatcac gctgaagagc aggaagaagg
     1081 tgacgacgat cggcaggacg atcgagaagg cgtagctcag cgccatggtg acggcgccgt
     1141 actcaccgac cagcaggtcg tgcccgaagc gcgcggcccg ctgccacgac gagggctctc
     1201 cgacgagccg ataggcgacg ccgctcacct cgcggctcca gctcgcgatc tccacttcgt
     1261 gcaccgggct caagggcaga gccaccgtcg cctccgctga ggcggtctcg tgaacgtgcg
     1321 gaaacggcag cagcgagtcg gcgacccgaa tcgccagcgg cgtaacgcgc tgctcgaaaa
     1381 cgccggtctc gagaagatcg accagcgtcc cggcgccgag cagaccgacg aaccagaaga
     1441 cgaccgccag cgccagggcg agaaacacca gccccttgac tggatgcatc gcccagaagc
     1501 cgagccgcac ggcgagggac gcgtgccgcg gctgcgtgat cgagtaggtc tgctcgagga
     1561 tctcgttgac gcgactcagg agcagcttgt tgcgctggta ctcgtcgtcg agtgccgtcg
     1621 ctccctcgcc gttcaggcgc ggcacggccg cgcgctcgag cgcggcgcgc agctcctcgg
     1681 tgccgcgatt ggtgatagcg accgccgaga ccaccggcac gccgagaatc gcctcgagcc
     1741 gttcgcgatc gatacgccct ccccgctgat cgagctcgtc ggtcatgttg aggacgagca
     1801 ccatgggtcg ggcgaggtcg ccgagctgca gagtcaggag cagcgcgcgg cgtaggttct
     1861 tcgcgtcggc cacctgcacg acggtcgcgt ccggcgcctc gtcgagcagc tggcgcgtga
     1921 cgcgcgcctc ctccgagcgc ggcgcgagat cgttgagtcc gggggtgtcg atgatctcga
     1981 aggactcctc cacgccgcgc gcccgcgccc ggacgacgtc gaccgtcgtc ccgggaaagt
     2041 tggaaaccgt cacgtaccgg ttggtgaggt tgcggaagag gacgctcttg ccgacgttgg
     2101 ggttgccgac gagcagcacc gggcgcggct tgctgtgtcg agagcgtcgt tccggaatgc
     2161 tcacccctgg ctcctggccg gcctcggccg gcctgcggca aatggtacta aattcggcct
     2221 gatttcaggg cagcggcgac accagacgtt ctcgggacgc gacaagccga agctctcagg
     2281 tgctcgctcg agccaaccga gactacgagt cgggtcacct actcgccggc cgatagctga
     2341 gccagcgcct tgcccgccgc gatcgcggcg cgattctcgt cccggtgccg ggccggcccg
     2401 ttgtccgcgg ccgccatcag cgactcctcg gagagagcct cgaggtcgag gagtgccgcc
     2461 gtcaggagcg ccagtgatcg cccggtgcgc cccaccgatc cggccgaagc gagatcgaga
     2521 gcgatcactc gcgcggcggt ctcgacggcc ggcagcgagg cctccacgta gaccgtgtcg
     2581 tcgggaccca tcgcgctcag atagcgtcgg gcctttccca gaccctcggc tgccagcagg
     2641 atcgtcgcgt cgggcttctc gatcccggtg taggcgaacg gctcgggcga gagaatcagc
     2701 tccgagatgc tgtgcccggt ctggaccgtg accggatagt cttctcgctg cgccgcccac
     2761 agtcccgaga ggatcgccgc gcggccggcc agccgggccg ccgagcgcac cttgccgccc
     2821 gccgcaccgg ccccgaccag gcgcatcggt cgcgccagcg acgccgcgaa gcgccgttcg
     2881 atcggctcgg cagcgagcac cggacgaccg cgcagctcgg cgtgcgctgt acggtaggcc
     2941 gtctcgtacc cctcgacctc gcggcgagcg atcaccccgg ccgccaggtc gagctcgtcg
     3001 agcatctcga acagcgcctt cttcgaagac ttgttgcgcg gcacgtagta ggcggtgcag
     3061 agctcccaga tgtcgagcag ggcgaacccg ggcgaggcga tcgcctcggc gatccgcgct
     3121 cccagatcgc ggtcgaagct cgtgccccgg tagacgtagg ccgcgccgtt gaccgccacc
     3181 gtgccgcaga cgtcgagcgg acgctcgagg ttgccgagcg gcgtggtcga ggtcgcggcg
     3241 cccgccggcg tcgtggtcga atgctgccct ccggtcatcc cgaagttgaa gttgttgaag
     3301 accaggaccg tcatgccgat gttccggcga gcagcgttga tgaggtgcgc gccgccgata
     3361 ccggtgccgc cgtcgcccat gacgacgacg acctcgagct ccggtcgcgc cagcttgatg
     3421 ccggaagcgt aggcgatcga gcggccgtgc aggccgtgaa acgcgttggt agcgaaatac
     3481 tgatccgaca gccccgagca gccgatgtcg gaaacgatga cgatctgctc ggggtcgcgc
     3541 tgcagctcga cgagcgccgc gttgagagca tccagaatgt tgccgtggcc gcagcccggg
     3601 cagaacggat acggtgtctc gcggcggtag gtcgcaagcg gcgtcaccgt ggtcggggga
     3661 gcgctcacgt caaggtctcc aggaaggcgc tcggcgccag cagctcgccg tcgttgcggt
     3721 gaagacccac gatacgctcg cgaccgacca gccgctccac ctcgcgtcgg tactggccgt
     3781 ggttcagctc ggcgacgacg atccggtcgg cgccctcggc ggccgcgacg atttcctcct
     3841 cgggcaccgg ccagaggctc tgaatcgtca gggccgagac cgcacgacca ttccgtcgcg
     3901 ccatcgacac cgcctcgcgc atcgcgcccg ccgtcgagcc ccaggagatc cacagcaccc
     3961 gggctttgcc gtcgaggtcg ctcgtaacca ggtcgatctc gtcgcgatgc gcctcgattt
     4021 tttcgacgag gtggcgattg agtttttcga ccgtcgcccg gtccttggtg atgaaggccc
     4081 gctcgtcgtg ggtcgagccc gtgaacctga ccttgtctcc ctcgccgaaa tgggcgatcg
     4141 gcggcacgtc gccggccggc tcgtagcggt agggtcggaa gtcgcccgac gtgtcgcgct
     4201 cggcgctccg ccgggcgcgt gcacggaccg ggacctccac gtactcgtcg aggtcgacgg
     4261 tcaccgccgt catgttgagc tccttgtcgg tcaagagcag caccgggcag cggaagcgct
     4321 cggcgagatc gaacgccttg cgcgtcaggc tgaagcactc gagcaccgag gacggcgcga
     4381 gcacgatcag cgggtagccg cccgcggtcc cccagcgcgc gaactg
//
LOCUS       DBNGNK010000079         6026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_585330,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000079 DBNGNK010000000
VERSION     DBNGNK010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_585330"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC150V_01980"
     CDS             complement(<1..242)
                     /locus_tag="AC150V_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HIR3761722.1"
                     /translation="MSFREDGSAAVEWVASYLEQVRDKPVLARVEPGEIRAALPASPP
                     EEPEPFRAVLDDLDSVLGPGLTHWQSPRFFAYVAVTG"
     gene            complement(239..1948)
                     /gene="polX"
                     /locus_tag="AC150V_01985"
     CDS             complement(239..1948)
                     /gene="polX"
                     /locus_tag="AC150V_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR3761723.1"
                     /translation="MSDALPRNAELADQLELLADLSEILGEQGFRVLAYRRAAARIRE
                     TPTSVAELALEGRAKELSGIGRTIEQKVVEVVEDGEMHALTRRREQVPDGVAEFMRLP
                     GLGPKTAARIWSTLGVTTLEELQQAAGDGRLRAVPGLGARSEQRILAALEQGAGETTP
                     ARGLLGVGLPVVREVAEALREHEAAVEVSEAGSVRRRRETFRDLDLIATASDPAALTA
                     HFVAQPWVVEVVAHGDTKATVVSQQGLRLDLRVVPPESYGDLLQHFTGSKEHNVALRE
                     EAVRRGLSVSEYGVTEAETGEVHRFTDEAGLYAFLGYAYIPPELRENGGELEAARAGG
                     LPALVEIGDLRGDLHSHSTWSADGKAKIEEMAEAAVARGYEYLAVTDHSHYLREGRIE
                     LQAEEIAALNGRLAPLRLLHGVEVNIRADGELDVADDVLAGLDWVVASVHTAFERSPT
                     ERIVGAIENPNVDCIGHLTGRRLSRREGANVDVDRVVEAAVATKTALEINSQPDRLDM
                     RDANARLAAEAGAWICVNTDAHSVKALDYAELGVSQARRAWLTREQVLNTRGWKQIEK
                     MRT"
     gene            1991..2722
                     /locus_tag="AC150V_01990"
     CDS             1991..2722
                     /locus_tag="AC150V_01990"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR3761724.1"
                     /translation="MRGVTRAFVCLPTYNEADNVEAMVGALAGVLDTARDRVLIIDDA
                     SPDGTGRLADALARDHSWVEVLHRGRKDGLGPAYIAGFEHALGAGAELVLEMDCDFSH
                     DPADVPRLIAAAEHADLVLGSRYVGGGGVESWGPIRRFVSRGGSVYARTLLSVPIADL
                     TGGFKCFRRRVLETIDLEACKTRGYAFQIELTYRAIAAGFRVVEIPIRFADRRAGSSK
                     MSGAIVAEAVWRVPSLRLRRATGRL"
     gene            2759..3082
                     /gene="trxA"
                     /locus_tag="AC150V_01995"
     CDS             2759..3082
                     /gene="trxA"
                     /locus_tag="AC150V_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3761725.1"
                     /translation="MPPVDVTDTTFDDEVLASPTPVIVDFWAPWCRPCKAIEPILEEI
                     ADKAGERVRLVKLDVDSNLGVSSRYGVLSLPTVMLFADGEPKATVVGAASRKTFEREF
                     ESWLG"
     gene            complement(3092..4138)
                     /locus_tag="AC150V_02000"
     CDS             complement(3092..4138)
                     /locus_tag="AC150V_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761726.1"
                     /translation="MLAFLIGRLVWAVMLFFVVTLYTYVLFFLIGPTSVNIGNRSAGE
                     SGEAVSVAESVGVEGSFFGAYASFLRKVVTGDLGQSFFSRQDVTEIIGAAAPVTGSLI
                     VGAAIMWFLIAFPIGVLSALNPRSLLDRVGMIFVLIGISAHPLWVGYIFSYVFGYRLG
                     IFPINGYCDVFNASTSCGGPVQWAYHLFLPWFTFALAFAAIYARMIRASLKETLAEDY
                     IRTARAKGLGTWPTVRGHAMRNALLPIVTMLGMDLGLAFVAAVFVERAYSLPGIGSLL
                     FTALGRHDTPVILGIVILVTTAIIVFNLIVDLVYGLLDPRVRPVRSRVRRSETSTEVP
                     TSTGSRQPSPATSS"
     gene            4192..4569
                     /locus_tag="AC150V_02005"
     CDS             4192..4569
                     /locus_tag="AC150V_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3761727.1"
                     /translation="MDADPTAARFLAALERRDFAAIGDCFAPDATFKALTPRQLREHV
                     GPGDIAARYDFWFAKLERFELLEAEAVAISDRVRLRYRVRGRHPEHGWWINEHTAYAA
                     LSGGRIASLSLTCTGFRPTEPPA"
     gene            4580..>6026
                     /locus_tag="AC150V_02010"
     CDS             4580..>6026
                     /locus_tag="AC150V_02010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3761728.1"
                     /translation="MSLIATGWADLLEGDELAHIAVEPAREACFSPLPDDLDPGLAAA
                     LVGQGITALYAHQAEAWAATRSGGDVIVTTGTASGKSLAFNLPVLDAIAAEPKSRALY
                     LYPTKALAQDQARSLAQLRPPGLKAAIYDGDTEAERRWQIRKWANVVLTNPDMLHVGV
                     LPHHDRWGDVLHNLRYVVVDEAHVYRGVFGSHVANVLRRLLRLARVYGADPQLLFASA
                     TIANAGELASSLTGRPAVVVAGEGAPRAEREIALWNPPLVDEELGLRASALGEASRLL
                     AGLVSRGLRTICFAKSRKAVELIHRFTCERVDSETAARLAPYRAGYTPAQRREIERRL
                     VAGELLGVSATDALELGIDIGLLDCAISVGFPGTVASLRQQWGRAGRRGRGLAVLVAS
                     EDALDQFFMREPDALLSRHVEAALLDHANPRILDPHVYAAAFEGPLDEADTATLGKVT
                     LERAAQLPELEETPAGWVWKGRDYPAARVSLR"
BASE COUNT          928 a         2133 c         2061 g          904 t
ORIGIN      
        1 ccggtcacgg caacgtaggc gaagaagcgc gggctctgcc agtgagtcag ccccgggccg
       61 agaaccgagt cgagatcgtc gagcacggcg cggaacggct cgggctcctc ggggggagag
      121 gccggcagag ccgcacggat ctcgccgggt tcgacccggg cgagcaccgg tttgtcgcgc
      181 acctgctcga ggtacgaggc cacccattcg actgcggccg atccatcctc gcgaaagctc
      241 acgtgcgcat cttctcgatc tgcttccagc cgcgggtgtt gagcacctgc tcgcgcgtca
      301 gccacgcccg gcgtgcctgg ctgaccccga gctccgcata gtcgagtgcc ttcacggagt
      361 gggcatcggt gttgacgcag atccacgcgc ccgcctcggc ggcgagtcgc gcgttcgcgt
      421 cgcgcatgtc gagccggtcg ggctgcgagt tgatctccag cgccgttttg gtcgcaaccg
      481 ccgcctcgac gacacggtcg acgtcgacgt tcgcgccctc gcggcgagaa aggcgccgac
      541 ctgtcaggtg cccgatgcag tcgacgttcg ggttctcgat cgcgccgacg atgcgttcgg
      601 tcggactgcg ctcgaaggcg gtatgcacgg acgccaccac ccagtccaga ccggcgagga
      661 cgtcgtcggc cacgtcgagc tcgccgtccg cgcggatgtt cacctcgacg ccgtgcagca
      721 gtcgtaacgg cgccagccgc ccgttcagag cggcgatctc ctccgcctgg agctcgattc
      781 ggccctcgcg cagatagtgc gagtggtcgg tcacggccag gtactcgtag ccgcgtgcga
      841 ctgcggcttc ggccatctcc tcgatcttgg ccttgccgtc ggcggaccag gttgagtggc
      901 tgtgcaggtc gccacgcagg tcaccgatct ccacgagggc cgggagcccg cccgcacgcg
      961 ccgcttcgag ctcgccgccg ttctcgcgca gctccggcgg gatgtacgcg tagccgagga
     1021 atgcgtacag cccggcctcg tcggtgaagc gatgcacctc gccggtctcc gcctccgtga
     1081 ccccgtactc ggagacggag aggccacgcc gcaccgcctc ctcgcgcagc gccacgttgt
     1141 gctccttcga gcccgtgaag tgctgcaaga ggtcgccgta gctctcgggc ggcacgaccc
     1201 ggagatcgag gcggagcccc tgctgcgaca ccaccgtcgc cttcgtgtcg ccgtgcgcga
     1261 cgacctccac gacccacggc tgtgcgacga agtgggcagt cagagcggcg ggatcggagg
     1321 cggtcgcgat caggtcgaga tcgcgaaagg tctcgcgccg gcgacgcacg ctgcccgcct
     1381 cggagacctc gacggcggcc tcgtgctcac gcaaggcctc ggcgacctcg cgcaccacgg
     1441 gcaacccgac cccgagcaga ccgcgcgccg gcgtggtctc gccggctcct tgctcgagcg
     1501 ccgccaggat cctctgctcg ctgcgtgccc cgagaccggg cacggctcgg aggcgaccgt
     1561 cgccggcggc ctgctgcaac tcctccagcg tcgtcacgcc gagagtcgac cagatccggg
     1621 cagccgtctt cggcccgagc cccgggagac gcatgaactc cgcgacgccg tccggaacct
     1681 gctcgcgccg ccgcgtcaac gcgtgcatct ctccgtcctc gaccacctcg acgaccttct
     1741 gctcgatcgt cctgccgatc cccgagagct ccttcgcacg gccttcgagg gccagctcgg
     1801 cgaccgaggt cggggtctcg cggatccgag cggcggcccg gcggtaggcg agcacgcgga
     1861 agccctgctc gccgaggatc tccgacaggt cggctaggag ctcgagctgg tcagccagct
     1921 ccgcgttgcg tgggagcgcg tcggacatcc cgccgatcat agggacggcc gcacggtgca
     1981 gaatcacggc gtgcgcggag tcacacgagc gttcgtctgc ctgccgacct acaacgaggc
     2041 cgacaacgtc gaggcgatgg tcggcgccct cgccggcgtc ctcgacacgg cacgcgaccg
     2101 cgttctcatc atcgacgacg cctcgcccga cggaaccggc cgactggcgg acgcgctcgc
     2161 ccgcgaccat tcctgggtcg aggttctcca ccgcgggcgc aaggacggtc tcggcccggc
     2221 ctacatcgcc ggtttcgagc atgccctcgg ggccggggcg gagctggtgc tcgagatgga
     2281 ctgtgacttc tcacacgatc cggccgacgt gccgcggctg atcgcggccg cggagcacgc
     2341 cgacctcgta ctgggctcgc gctacgtcgg cggaggcggc gtcgaaagct ggggccccat
     2401 ccgccggttc gtctctcggg ggggctccgt atacgcccgc acgctgctgt cggtcccgat
     2461 cgcggacctg acgggcggct tcaagtgctt tcgccgccgc gtgctcgaga cgatcgatct
     2521 cgaggcctgc aagacacgcg gctacgcatt tcagatcgag ctgacctacc gcgcgatcgc
     2581 cgcgggtttt cgcgtcgtgg agatcccgat tcggttcgcc gatcggcgcg ccggcagttc
     2641 gaagatgagc ggggcgatcg tcgcggaggc tgtctggcgc gtgccgtcgc tgcgcctccg
     2701 tcgcgcaacc ggtcgtctct gagtcgaagc gcgtcctgct cgcatctaag ctctccgaat
     2761 gcctccggta gacgtaaccg acacgacctt cgacgacgag gtgctggcgt cgccgactcc
     2821 ggtcatcgtc gacttctggg ctccctggtg ccggccgtgc aaggcgatcg agccgatcct
     2881 cgaggagatc gccgacaagg ccggcgagcg ggtcaggctc gtcaagctgg acgtcgactc
     2941 caatctcggc gtctcctccc ggtacggcgt cctgtcgctc cccaccgtca tgctgttcgc
     3001 ggacggcgag ccgaaggcga cggtcgtcgg cgcggcctcg cgcaagacgt tcgagcgcga
     3061 gttcgagagc tggctcggat gagcggcctc gctacgagga cgtggcgggc gacggctgcc
     3121 gactaccggt ggaagtcggc acctcggtcg atgtctccga gcgtcggacc cggcttctga
     3181 caggccgcac acgcgggtcg agaagcccgt agacgaggtc gacgatcaag ttgaagacga
     3241 tgatcgcggt cgtcacgaga atgacgatgc cgaggatcac cggcgtgtcg tggcggccca
     3301 gggcggtgaa caacaggctg ccgattcccg gcaggctgta ggcgcgctcg acgaagaccg
     3361 ccgccacgaa ggccagcccg agatccatcc ccagcatcgt cacgatgggc aggagcgcat
     3421 tccgcatcgc gtggccacgg acggtgggcc acgtcccgag ccccttcgcc cgcgcggtgc
     3481 ggatgtagtc ctccgcgagg gtttccttca ggctggcgcg gatcatgcgc gcgtagatcg
     3541 ccgcgaaggc gagcgcgaag gtgaaccagg gcaggaagag gtggtatgcc cactgcaccg
     3601 ggccgccgca tgacgtggac gcgttgaaca cgtcgcagta cccgttgatc gggaagatgc
     3661 ccagcctgta gccgaagacg tacgagaaga tgtagccgac ccagagcggg tgcgccgaga
     3721 tcccgatgag gacgaagatc attccgaccc ggtcgaggag cgatcgcggg ttcagtgccg
     3781 aaaggacgcc gatcgggaag gcgatcagga accacatgat ggcggcgccg acgatgagcg
     3841 atcccgtcac gggggcggcc gcgccgatga tctccgtgac gtcttggcgt gagaagaacg
     3901 actggcctag gtcgccggtg acgaccttgc ggagaaagct cgcgtaggca ccgaagaacg
     3961 agccctcgac accaacggac tcggcgaccg agacggcctc gccgctctct cccgcgctgc
     4021 ggttgccgat gttgacgctc gtcggcccga tcaggaagaa cagcacgtag gtgtagaggg
     4081 tgacgacgaa gaacagcatc accgcccaga cgagccgccc gatcaggaat gcgagcaccg
     4141 gctcccaaga gtgacgaaca cctgcgggtc tgtgcaaccc tgggcagggc aatggacgcc
     4201 gacccgacgg ccgcccgctt cctcgcagcg ctcgagcgtc gcgacttcgc cgcgatcggc
     4261 gactgcttcg ctccggacgc caccttcaaa gccctcacgc ccaggcaact gcgcgagcac
     4321 gtcgggccgg gggacatagc cgcccgctac gacttttggt tcgcgaagct cgagcgcttc
     4381 gagctgctcg aggcggaggc cgtcgcgatc agcgaccggg tgcgtttgcg gtaccgcgtg
     4441 cgcggacggc atccagagca cgggtggtgg atcaacgagc acaccgccta cgcggcactg
     4501 agcgggggtc ggatcgcctc gctgagtctc acctgcacgg gcttccggcc tacggagccg
     4561 cccgcgtgac gggagtcgcg tgagcctgat agcgaccggc tgggcggacc tgctcgaggg
     4621 agacgagctc gcgcacatcg ccgtcgagcc agcgcgggaa gcctgcttct ctccgctccc
     4681 ggacgacctc gatccgggac tggcagcagc gctcgtcggg caggggatca cggccctcta
     4741 cgctcatcag gcggaagcct gggctgcgac caggtccggc ggcgacgtca tcgtcacgac
     4801 gggcaccgcc tccggcaagt cgctcgcgtt caacctcccc gtgctggacg cgatcgcggc
     4861 cgagccgaag tcgcgtgccc tctacctcta cccaacgaag gcgctcgctc aagaccaggc
     4921 ccggtcgctg gcgcaactgc ggccgcccgg gctcaaggct gcgatctacg acggggacac
     4981 cgaggccgag cgacgctggc agatccgcaa gtgggccaac gtcgtcctca cgaacccgga
     5041 catgctccac gtgggcgtgc ttccccatca cgatcgatgg ggcgacgtcc tccacaacct
     5101 gcggtacgtc gtcgtcgacg aagcgcacgt ctatcgcggc gtcttcgggt ctcacgtcgc
     5161 gaacgtgctc cggcggctgt tgcgcctcgc ccgcgtctac ggcgccgacc cgcaactcct
     5221 gttcgcctcg gccacgatcg ccaacgcggg cgagctcgca tcgtcgctca ccggacgccc
     5281 ggcggtcgtc gtcgccggag agggagcccc gcgggcggaa cgggagatcg ccctctggaa
     5341 cccgccgctc gtcgacgagg agctcggcct ccgcgcgagc gcgctcggcg aagcgtctcg
     5401 cctgctggcg ggcctcgtca gccgggggct gcgcacgatc tgcttcgcca agagccgcaa
     5461 ggcggtcgag ctcatccatc gcttcacctg cgaacgggtc gactccgaga cggccgcgcg
     5521 actcgctccg taccgcgcgg ggtacacccc ggcgcaacgc cgtgagatcg aacgccggct
     5581 cgtcgccggc gagcttctcg gcgtgtccgc gacggatgcg ctcgagctcg gcatcgacat
     5641 cggcctgctc gactgtgcca tctcggtcgg gtttcccggc accgtcgcgt cgctccgcca
     5701 gcagtggggg cgcgcagggc gtcgcggtcg cgggctcgcc gttctcgtgg ccagcgaaga
     5761 cgcgctcgac cagttcttca tgcgtgagcc cgacgctctc ctgtcacgcc atgtcgaggc
     5821 ggcgctgctc gaccacgcaa acccgcggat cctcgaccct cacgtctacg cggccgcgtt
     5881 cgagggcccg ctcgacgagg ccgacaccgc gacgctgggc aaggtgacgc tcgagcgtgc
     5941 ggcgcagctc cccgagctgg aagagacgcc ggccggctgg gtctggaagg ggcgcgacta
     6001 ccccgcagcg cgcgtctcgc tccgct
//
LOCUS       DBNGNK010000080        14242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1159684,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000080 DBNGNK010000000
VERSION     DBNGNK010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 14242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14242
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1159684"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="AC150V_02015"
     CDS             complement(<1..1074)
                     /locus_tag="AC150V_02015"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR3761729.1"
                     /translation="MSYVELHCHSAYSFLDGASLPEELAARAAELGYEALALTDHDGV
                     YGSLEFAHAAKALGVRPITGAEVTLAGGAHVTLLCESQRGYANLCGILTAAHAGTRPD
                     GGAEPLPPEVEQDVVAEHAAGLVCLSGCARHGLAVRDPNAAERLSAAFGRGRFFVELQ
                     RPYERGDRRRNALLRDLATHLHVDTIVTGNVHAHELRRTLLQDALVAVRCRTSLDGCE
                     RERRGNRESVLRAPFEVADRFDACDLAAVERTVSLAERLEFDLTQELGYRYPDFSDGD
                     RPAIRQLAAICEAAFTERYPAGNSALLARARTRLEEELRLIDELELAGFFLLHWEVLE
                     LARQIALEVRGAGSPRRFLPPGRG"
     gene            1086..1937
                     /locus_tag="AC150V_02020"
     CDS             1086..1937
                     /locus_tag="AC150V_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3761730.1"
                     /translation="MEPLLVVDLPERTVSIRIALEDVVVTESRYVAGERGPELHVHRL
                     HADCFYVLAGSFTLVLDDGEHVLDAGTFALVPPNVVHAFRNDGPGELRFLNLHAPGMS
                     LDRYVQEISATGEEFRTALAARYDQHPPPAAGGLDPGSVIVRTADSDAVTVAGIHNGY
                     LAHGHEALDAMGLVEYTAPPLFAGPPQHVHDRTWDAYYVLEGRLAVSVGGEQRELGPG
                     DVVVVPPGTAHGFSNPDSSPTRFLDIHVPGGFERYYREVAAATGEGPLDPAEMARIGS
                     RYDVRPV"
     gene            1947..2294
                     /locus_tag="AC150V_02025"
     CDS             1947..2294
                     /locus_tag="AC150V_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761731.1"
                     /translation="MDTRERYEDICDALTAWNPDVRRSKIFGMPCLKRGRRVACGFPR
                     GERAMVFRLRDPEAHARALALPGAHLFDPSGRGEPFRDWVVVPAEHGAAWEALAYDAV
                     TQELELSPPSMDP"
     gene            complement(2318..2569)
                     /locus_tag="AC150V_02030"
     CDS             complement(2318..2569)
                     /locus_tag="AC150V_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761732.1"
                     /translation="MTGRRAGRLNAPRPALVETSFAGTPNMVNRQPVALVREEWRVVY
                     RWWTDEPVGRRYFDVVLETGQNVCVYRDTERAAWFTQRA"
     gene            complement(2650..3834)
                     /locus_tag="AC150V_02035"
     CDS             complement(2650..3834)
                     /locus_tag="AC150V_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR3761733.1"
                     /translation="MIACLLVPGFELRAALRSRPRLAHSPTALAPPADAEQLIGAVTV
                     AAESAGVLPGMRLGEALATCPGLVLVEPDPAAVEQEWEAMLRRLEDAGFAVESEEPGA
                     VYFDTRGVERLYGGVEAALKRALAAAGTAWDPRAGAGKRRFAALAAASVAHPGQALIV
                     EGEAERDFLEPLPLSLLSLPPDRYAELEGLGVQRLGELARLPGEAVAERLGPEGRKAW
                     KLARGGERRRVRGRAPAVELAESLSFPEAVGNHITLARAFRALFDRLLARPERAGRPF
                     RKIAVSARLVGGGSWRRALTLREATADRGRLRAALAPKLLEIPGPVVALRLEAVELAE
                     HTGNQLALVEPEGEELDARLREGLRQVRASTGTGSVCAVVEVAPWSRIPETRALVVPR
                     DV"
     gene            complement(3882..4622)
                     /locus_tag="AC150V_02040"
     CDS             complement(3882..4622)
                     /locus_tag="AC150V_02040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3761734.1"
                     /translation="MVVNRDSRRGRAESFSSVADAYERGRPEYPADALRWLTGDAPVD
                     VIDLGAGTGKLTRGLLALGHRVTAVEPLEEMLGQLRRASPGVTAVAGHAERIPLSAGS
                     ADVVAVGQAFHWFDAAAALPEIARVLRPGGRLALVWNTRDDREPWVARLSKLIGSETV
                     AAVDAEVPIDESPLFTPLESAQFGSEQRLERETLRDLVLSRSYCATRTPAERETVLAE
                     VDRLYDEVSGPDGVVLPYVTDCYRGVRR"
     gene            complement(4630..5328)
                     /locus_tag="AC150V_02045"
     CDS             complement(4630..5328)
                     /locus_tag="AC150V_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR3761735.1"
                     /translation="MHVHVAFTPDESAAAPTGVVIDVIRATSTICQALASGYRHVFCA
                     ADVDEVRDLARALGDVTTGGERLGLRIEGFELGNSPLEYVRPHAATLAMTTTNGTRAI
                     LTAADRCERVLVGSLLNLSAVAAAAREAGGDVAVFCAGVEGSFALDDAYVAGRITELI
                     DGERSDAAEAAVRLAVSYPSAEEAFRRSLSGRKLIEAGPELEADIPYCARESVLDIVP
                     QLLGMRGSAAEIAL"
     gene            5434..6186
                     /locus_tag="AC150V_02050"
     CDS             5434..6186
                     /locus_tag="AC150V_02050"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR3761736.1"
                     /translation="MTFAPVGIRCPEHANLGAAKPSAQRTVRQTRGRVASSAAPATLV
                     LVGINVLVYLITVAQGGGLNAPGGELFQRGALVGIFVADGDWYRLVTAMFLHAGLIHL
                     MFNMLALWWLGSIVEQAIGTWRYLLIYFASGLAGSAGALLLSGPLAITVGASGAIFGI
                     LGGLLVLEWQHTGSIAGPALTLIVLNLAITFTIPNISIGGHLGGLVGGTLATFALVRT
                     RFTGNVMLGPAVAVLIGLASVALAYFRVENYL"
     gene            complement(6202..8799)
                     /gene="ppdK"
                     /locus_tag="AC150V_02055"
     CDS             complement(6202..8799)
                     /gene="ppdK"
                     /locus_tag="AC150V_02055"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR3761737.1"
                     /translation="MKYVYSFEEDAGADRDLLGGKGAGLAEMTALGVPVPSGFTITTD
                     ACRAYLANGGTVPDGLDAEVDAHVAALEEKSGKRFGDDSDPLLVSVRSGASISMPGMM
                     DTILNLGLNDTSVAGQTAATGNARFALDSYRRLVQMYGSVVEGVAGTRFERALAELKA
                     DRGVSQDVDLTAEDLEDLVKTFLAIYAEDAGHDFPQDPREQLRRAYRAVFESWNTHRA
                     QVYRRTYAIPDDLGTACNVVEMVFGNKGDRSATGVCFTRDPANGEKRLYGEFLRNAQG
                     EDVVAGIRTPEPIERMAEELPEAYAELVDTLDRLEAHFRDVQDIEFTVEEGTLYLLQT
                     RTGKRTAAAALRIAVEMVDEGLISREDAVARIDPEQLDQLLHPMIDPSATIEVAAKGL
                     NASPGAASGAIVLDADTAEEHGKLGESVILVRWETMPDDIHGLIEARGIVTAHGGMTS
                     HAAVVARGMGKPCVAGCEALSIDAAAGTVQIGDSELREGDIITIDGTSGSVIVGSVPL
                     VSPSIDENFSTILRWADDLRRLGVRANADTPEDAMKAREFGAGGIGLCRTEHMFMAED
                     RLPIVREMIMARTEDERRAALDRLLPMQQADFEGIFAAMTGLPVIIRLLDPPLHEFLP
                     PLEEATTEDMRSRIKQLRESNPMLGTRGCRLGLQFPEIYEMQVRAIVRAARAVRERTG
                     EAPIVEVMHPLVGFGEELARLRELTERIAAEEPTVEYLCGTMIELPRACVRADEIAQL
                     ADFCSFGTNDLTQTALGFSRDDAEGKFLTHYLEQGILKRNPFEAIDIEGVGDLMRLAV
                     ERARGVNPGISLGLCGEHGGEASSVAFCHEIGLDYVSCSPYRVPLARLAAAQAALAEA
                     GVTAAVSGG"
     gene            9082..9645
                     /locus_tag="AC150V_02060"
     CDS             9082..9645
                     /locus_tag="AC150V_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR3761738.1"
                     /translation="MSRRAFPHSVRTTTPRRVVLVSSASGNGKTTIGRLLARRLGVPF
                     HELDALVHGPNWLETPDNELRVLVGRIVATDAWVVDGSYQRKLGDLVLEHADLVVWLD
                     LPIRVWLPRLMRRTVRRTRRREVLWNGNRETYRHTVVGWDSLFVYALRAHFRRRREYP
                     VRLAAYPTVRLRSRDAVERWLSLVAGL"
     gene            complement(9623..9835)
                     /locus_tag="AC150V_02065"
     CDS             complement(9623..9835)
                     /locus_tag="AC150V_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761739.1"
                     /translation="MSEGNERFGTLARGVERDMTEKGWAPARFWALWSVQIVLGVLVF
                     YVLLTPVWLGLRVAAWVAEFRARRRG"
     gene            complement(9832..10671)
                     /locus_tag="AC150V_02070"
     CDS             complement(9832..10671)
                     /locus_tag="AC150V_02070"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HIR3761740.1"
                     /translation="MATEDRAARQAVELHIVSDSTGETAARLVLALEAQFPEQPFAEV
                     RHPRCETAADLQRAVAQAKGRPAVMVYTLVEPKLREEMRRLCRSARLHYCDLLGHPID
                     SISRVAGVAASMEPGARPPLDDTYFRRIEALEFAVRYDDGLGGSLDEAEIVLVGVSRS
                     SKTPVSMYLGYLGHKTANVPIVKGVEPPPALFEIDPLKIVGLTIDPDRLSEFRRARVR
                     SMSAPRQYAEMSEIFEELELAAAVHRRLHCPVVDVSDLSVEGTAMQVIRLIDRRRRES
                     EVA"
     gene            complement(10707..12530)
                     /gene="glyS"
                     /locus_tag="AC150V_02075"
     CDS             complement(10707..12530)
                     /gene="glyS"
                     /locus_tag="AC150V_02075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR3761741.1"
                     /translation="MATLLLEIGCEELPAVAVYEAGEQLPRLVREHLDCEPSELFLGP
                     RRLAFLVRDLPDQIPEQWIKGPPVKVGEKAAAGFARKLGVAREDLVERDGVLGWLKPT
                     EPIAETLPGRLAAVIDGLTFGKTMTWEQGGRRFPRPLRWTLAKLDEQTLLGATSQGHR
                     FRSGPVEIQDADEYADRLRSHGVEPSLERRRAAIVEGLDRLGDWRDPMGKLDEVVNLV
                     EWPVVLEGKFEERFLELPERVVETAMQSHQRYFPLGGTRFAFVANGGDPHVVRAGNEQ
                     VLEGRLADAAFTFERDAAVGVEVLAQRLGVITFFHGAGSYADKTERLLPLVRSLGGDE
                     EAVEAARLAKADQASELVREFPDLEGHVGAVYARLAGKQEGVVAAIEEQYLPDAAGGP
                     LPSTAAGRVLAAADKLDTLNVAFSLGHKPTGSRDPYALRRAAIGLCRLAIEGGIPIEI
                     SDPEVRDFVQERLEALLDVPVEYVRAARSAGRDELGDVARLAEFLHALPSDQLATAHE
                     VYTRAARIAGDAADGAPYDRALAEEQAERELAEAVAAAPEVGNDAIMTWATGTAPLVA
                     RFFDEVLVMDPDPAVRANRLRLLRDLRDAVGRAGDLAQIPL"
     gene            complement(12511..13377)
                     /locus_tag="AC150V_02080"
     CDS             complement(12511..13377)
                     /locus_tag="AC150V_02080"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR3761742.1"
                     /translation="MPLNFQDMIAGLQTYWGGRGCVILQPYHTEVGAGTFSPATVLRC
                     LGPRPWRTFYVQPSIRPTDGRYGENPYRFQHYFQGQVILKPAPEDVLDLYFGSLEAIG
                     IDLTRHDVRLVEDDWEGPTLGAWGLGWEVWIDGMEVTQFTYFQQVGGLDLELIPAEIT
                     YGLERLAMFIQGKRSAFEVEWVDGVTWGDVYRENERQWSAYNFEHAPVDTLMGRFVEH
                     EAECRQLLDSGLPVPAYDQVLKASHAFNLLDARGAISVTERAAYIGRVRKLARAVCQA
                     YVAKEADGDAAA"
     gene            13478..13906
                     /locus_tag="AC150V_02085"
     CDS             13478..13906
                     /locus_tag="AC150V_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761743.1"
                     /translation="MRIAVLLAVAVPLTTADLVSKASGSIPPWAYHDRSFAWIVLCCG
                     LLVALVLITRVPSPLVPPAAGLLAAGVLGNALSAAWNGLSVPNPIIVRGDGAVLAFNL
                     ADVWVLVGIISLVSVLATWLVRNRSELPERLPVWARRRAG"
     gene            complement(13911..14168)
                     /gene="recO"
                     /locus_tag="AC150V_02090"
                     /pseudo
     CDS             complement(13911..14168)
                     /gene="recO"
                     /locus_tag="AC150V_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014607.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
     gene            complement(14140..14202)
                     /locus_tag="AC150V_02095"
                     /pseudo
     CDS             complement(14140..14202)
                     /locus_tag="AC150V_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002515784.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2158 a         5085 c         4783 g         2216 t
ORIGIN      
        1 tccacgccct ggcgggagga agcgtcgcgg cgagccggcg ccgcgcacct cgagcgcgat
       61 ctgacgagcg agttcgagca cctcccagtg gagcaggaag aagccggcga gctcgagctc
      121 gtctatcagc cgcagctcct cctcgagacg ggtgcgcgct cgggcaagga gcgcgctgtt
      181 tcctgccggg tagcgctctg tgaacgcggc ctcgcagatc gccgcgagct gccggatcgc
      241 cggccggtcg ccgtcggaga agtctggata gcgatagccg agctcctggg tgaggtcgaa
      301 ctcgagccgc tcggcgagcg acacggtgcg ctcgacggct gcgaggtcgc acgcgtcgaa
      361 gcggtctgcg acctcgaagg gtgcccggag aacgctttcg cgattgccgc gtcgctcgcg
      421 ctcgcagccg tccagagagg ttcggcaacg aacggcgacg agcgcgtctt ggagcaacgt
      481 tcgccgcaac tcgtgcgcgt gcacgttgcc ggtgacgatc gtgtcgacat ggagatgcgt
      541 cgcaaggtcg cgcagaagcg cgtttctcct cctgtccccg cgctcgtacg ggcgctggag
      601 ctcgacgaag aaccgtccgc ggccgaacgc cgcggagaga cgctctgccg cattggggtc
      661 acgaaccgcg agcccgtgac gagcgcatcc cgacaggcag accagccccg ctgcgtgctc
      721 ggcgacgacg tcctgctcga cctcgggagg gagcggctcg gcaccgccat ccggccgggt
      781 gccggcatgc gccgccgtca ggatgccgca gaggttggca tagccacgct gcgactcgca
      841 gaggagcgtg acgtgcgcgc cgcccgcgag ggtcacctcc gccccggtga tcggtcgcac
      901 tccgagtgcc ttcgccgcgt gggcgaactc gagcgagccg tacacgccgt cgtggtcggt
      961 gagcgcgagc gcctcgtagc cgagctcggc tgcgcgcgcg gcaagctcct ccggtagcga
     1021 ggcgccgtca agaaacgagt aggccgagtg gcagtgcaac tcgacgtagc tcatagactc
     1081 atcccgtgga gccgctgctc gtcgtcgacc tgccggaacg gaccgtgagc atccggatcg
     1141 cgcttgagga cgtcgtggtg accgagtcgc ggtacgtggc gggcgagcgt gggcccgagc
     1201 tgcacgtgca tcggctgcac gcggactgct tctacgtcct cgcggggtcg ttcacgctcg
     1261 tcctcgacga cggcgagcac gtgctcgacg cgggcacgtt cgcgctcgtg ccgccgaacg
     1321 tcgtgcacgc attccggaac gacggcccag gcgagctgcg cttcctcaat ctgcacgcgc
     1381 ccggaatgag cttagaccgc tacgtccagg agatctccgc cacgggtgag gagttccgga
     1441 ctgccctcgc cgcccggtac gaccagcatc cgccgcccgc agcgggcggt ctcgacccgg
     1501 gctccgtgat cgtccgcacg gcagattccg atgccgtaac ggtggccggg atccacaacg
     1561 gctacctcgc tcacgggcac gaagccctcg acgcgatggg cctcgtcgag tacacggcgc
     1621 cgcccctgtt cgccgggccg ccgcaacacg tccacgatcg cacctgggac gcctactacg
     1681 tgctcgaggg ccgtctggct gtgagcgtcg gcggagagca gcgggagctc gggcccggcg
     1741 acgtcgtcgt cgtgcctccc ggcacggcgc atgggttctc gaatcccgac agcagcccga
     1801 cgcgctttct cgacatccat gttcccggtg ggttcgagcg gtactaccga gaggtcgccg
     1861 cggccaccgg tgaaggcccg ctcgacccgg ccgagatggc gcggatcggg tctcgctacg
     1921 acgtccggcc cgtgtagccg caccacatgg acactcggga gcgctacgag gacatctgcg
     1981 acgcgctcac cgcctggaat cccgacgtgc gccgatcgaa gatcttcggc atgccgtgcc
     2041 tcaagcgagg acggagagtc gcctgcggct tcccccgcgg cgagagagca atggtgttcc
     2101 ggctgcgcga tccggaagcg catgcgcggg cgctcgccct cccgggagcc catctcttcg
     2161 acccgtccgg tcgtggcgag ccgtttcgcg actgggtcgt cgtgcccgca gagcatggag
     2221 ccgcgtggga ggcactggcg tacgacgcgg tcacccagga gctggagctc tctccaccgt
     2281 ccatggatcc ataacggcct gctacgtgcc cttcgcctca cgcgcgctgt gtgaaccacg
     2341 ccgcgcgctc ggtgtcgcgg tagacacaga cgttctggcc ggtctcgagc acgacatcga
     2401 agtagcggcg gcccaccggc tcgtccgtcc accagcggta gacgacacgc cactcctccc
     2461 gcacgagcgc gaccggctgc cggttgacca tgttcggtgt ccccgcgaag ctcgtctcga
     2521 cgagcgcagg ccgtggcgcg ttcaggcgtc ccgcccgacg cccggtcacc ggaggggcac
     2581 cgaggcgtcc cgcggcacga cgagcgctcg cgtttccgga gtccgcgacc gcctgctcat
     2641 cggcacgcct tagacgtccc gaggaacgac gagcgcacgc gtttccggta tccgcgacca
     2701 gggagccacc tcgacgactg cgcagacgga tcccgtgccc gtgctggcgc gaacctggcg
     2761 cagaccctcg cgcagccgtg catcgagctc ttctccttct ggctcgacca gcgcgagctg
     2821 gttgccggtg tgctcggcca gctcgaccgc ctcgaggcgg agagcgacga cggggccggg
     2881 aatctcgagc agcttcggcg ccagcgctgc gcgcaggcgt ccacggtcgg cggtcgcttc
     2941 ccgaagcgtc agggcgcgcc gccaggagcc gcctccgacc agacgcgcag agaccgcgat
     3001 cttgcgaaaa gggcgccctg cccgctccgg tcgtgcgagc agcctgtcga agagtgcacg
     3061 aaacgcacgg gcaagagtga tgtggttgcc gaccgcctcg gggaaagaga gcgactcggc
     3121 gagctccact gcaggagccc gccctcgtac gcggcggcgc tccccgccgc gcgcgagctt
     3181 ccaagccttg cgcccttcgg gccccagtcg ctccgcgacg gcctcccccg gtagccgtgc
     3241 gagctcaccc agtcgctgca cgccgagccc ttcgagctcc gcgtagcggt cgggcggcag
     3301 cgacagaagc gagagcggca agggctcgag gaagtcgcgc tctgcctccc cctcgacgat
     3361 cagcgcctgc ccggggtgcg cgacgctcgc cgctgcgagg gcggcgaacc ggcgcttccc
     3421 tgcgcccgcg cgtggatccc aggcagttcc cgcggcggcg agcgcccgtt tcagcgccgc
     3481 ctccacacct ccgtagagcc gctcgacgcc tctcgtgtcg aagtagacag cacccggctc
     3541 ttccgactcg acggcgaagc ccgcatcctc caagcggcga agcatcgctt cccattcctg
     3601 ctcgaccgcc gccggatcgg gctcgacgag cacgagcccc gggcaggtcg cgagtgcctc
     3661 ccccagccgc atgccgggca gcaccccggc agactcggcg gccaccgtca cggcgccgat
     3721 cagctgctct gcgtcggcgg gcggtgccag tgccgtcgga gaatgcgcca accgcggccg
     3781 ggagcgcaat gctgcgcgaa gctcgaatcc cgggacgagg aggcaggcga tcattgggaa
     3841 cacatgttct atacgaacac gtgttcccct gtcaagcgac cctagcgcct gacaccccgg
     3901 tagcagtcgg tgacgtaggg aagaacgacc ccgtccgggc cggaaacctc gtcgtagagg
     3961 cgatcgacct cggccagcac cgtctcccgc tccgccggtg tacgggtggc gcagtagctg
     4021 cgggagagga cgaggtcgcg gagcgtctcc cgctcgagcc gctgctcgga tccgaactgc
     4081 gcgctctcga gtggcgtgaa gagcgggctc tcgtcgatgg gcacttcggc gtccaccgcc
     4141 gcgaccgtct cgctgccgat gagcttgctc agccgtgcga cccagggctc gcgatcgtcg
     4201 cgcgtgttcc agacgagcgc gagccgtccc ccgggtcgga gaacccgtgc gatctccggt
     4261 agcgcggcag cagcgtcgaa ccagtggaac gcctggccga ccgcgacgac gtccgcgctc
     4321 ccggcggaaa gcggaatcct ctccgcgtgc ccggcgacgg cggtcacgcc gggggaagcg
     4381 cggcggagct ggccgagcat ctcctcgagc ggctcgacgg ccgtcacccg atgcccgagc
     4441 gcgagaaggc cgcgcgtcag ctttcctgtc ccggcaccga ggtcgatcac gtcgaccggt
     4501 gcgtcgcccg tgagccagcg aagcgcgtcg gccggatact cgggtcgccc ccgctcgtat
     4561 gcgtccgcga cactcgagaa agactctgcc cgcccgcgtc gcgagtcgcg gttcaccacc
     4621 atgccggact cacagcgcga tctcggctgc cgatccgcgc atgccgagca gctgcggcac
     4681 gatgtcgaga acgctctccc gcgcgcagta cgggatgtcg gcttccagct cgggcccggc
     4741 ctcgatgagc ttccggccgc tcagcgatcg cctgaaggcc tcctcggcac tcgggtagga
     4801 gacggccagg cgcaccgcgg cctctgcggc gtcgctgcgc tccccgtcga tcagctccgt
     4861 gatacgcccg gcgacatacg cgtcgtcgag cgcaaagctg ccttccacgc cggcgcagaa
     4921 gactgcaacg tcgccgccgg cctcgcgggc tgcggcggcc acggccgaga gattcagcag
     4981 tgacccgacc agcactcgct cgcaccggtc ggcggccgtg aggatcgccc gcgtgccgtt
     5041 ggtcgtcgtc atcgcaagtg tcgccgcatg cgggcgcacg tactccagcg gagagttgcc
     5101 cagctcgaac ccttcgatgc gaaggccgag ccgctcacca cccgtcgtga cgtcgcccag
     5161 tgctcgggca aggtcgcgta cctcgtcgac gtcggctgca cagaacacat gccggtagcc
     5221 ggaggcgagc gcttggcaga tcgtggacgt tgcccggatg acgtcgatca cgacgccggt
     5281 cggggccgcc gcggactcgt ctggcgtgaa cgcgacatga acgtgcacgc cggtacgcta
     5341 gccaccatgg atgccgagca ttgctatcgc catccggacc gtgaaacccg cgtcacgtgc
     5401 tccgaatgcg gccggccgat ctgcgaggac tgcatgacgt tcgcaccggt tgggatccgc
     5461 tgcccggagc acgcgaatct cggtgccgcg aagccgtcgg cacagcggac ggtgcggcag
     5521 acgcgcggcc gcgtggcgtc gtcggccgcg ccggcgacgc tcgtgctggt cgggatcaac
     5581 gtgctcgtgt acctcatcac ggtggcgcag ggcggcggcc tcaatgcacc cggtggcgag
     5641 ctcttccagc gcggcgcgct ggtcggcatc ttcgtcgccg acggcgactg gtaccggctc
     5701 gtgacggcga tgttcctgca cgcaggcctc atccacctga tgttcaacat gcttgcgctg
     5761 tggtggctcg gctcgatcgt cgagcaggcc atcggcacgt ggcgctacct gctcatctac
     5821 ttcgcctccg gtcttgcagg gtcagcggga gcgcttctgc tgagcggacc gctggcgatc
     5881 accgtcggtg cctcaggcgc gatcttcggc atcctcggcg ggctgttggt gctcgaatgg
     5941 cagcacacgg ggagcatcgc cggccccgcg ttgacgctga tcgttctcaa cctcgcgatc
     6001 acgttcacga tcccgaacat ctccatcggg ggccatctcg gcggccttgt cggtggaacg
     6061 ctggcgacct tcgcgctcgt gcggacgcgg ttcactggca acgtcatgct cggcccggct
     6121 gttgccgtgc tgatcggact ggcaagcgtc gcgctcgcgt atttccgggt cgagaactac
     6181 ctgtagccgg gctgctgttt ctcagccgcc gctgacggcc gcggtgacgc cggcttcggc
     6241 gagtgccgcc tgtgccgcgg ccaggcgcgc cagcggcact cgatagggcg aacaggacac
     6301 gtagtcgagg ccgatctcgt ggcagaacgc caccgagctc gcctcgccgc cgtgctcgcc
     6361 gcagagtccg agcgagattc ccgggttgac gcctcgcgcg cgctcgaccg cgagccgcat
     6421 cagatcgccg acgccctcga tgtcgatcgc ctcgaacggg ttgcgcttga ggatgccctg
     6481 ctcgaggtaa tgggtgagga acttcccctc ggcgtcgtcg cgcgagaacc cgagcgccgt
     6541 ctgcgtgaga tcattcgtgc cgaacgagca gaagtcggcc agttgcgcga tctcgtcggc
     6601 gcgaacgcat gctcgaggca gctcgatcat ggtgccgcac aggtactcga cggtgggctc
     6661 ctccgccgcg atccgctccg tcagctctcg tagccgcgcg agctcctcgc cgaatcccac
     6721 gagcgggtgc atgacctcga cgatcggcgc ctcccccgtg cgctcgcgga ctgctcgtgc
     6781 ggcccggacg atcgcgcgca cctgcatctc gtagatctcc gggaactgga gcccgagccg
     6841 gcagccgcgc gtcccgagca tgggattgga ctcacgaagc tgcttgatcc tggaccgcat
     6901 gtcctcggta gtcgcctcct ccaacggcgg caggaactcg tgcagcggag gatcgagcag
     6961 tctgatgatc acgggcaggc cggtcatcgc cgcgaaaatc ccctcgaagt ccgcctgttg
     7021 catcggcagg aggcggtcga gagcagcgcg tctctcgtct tcggtgcggg ccatgatcat
     7081 ctcccgcacg atcggcagcc ggtcttcggc catgaacatg tgctccgtcc ggcagagccc
     7141 gattccgcct gctccgaact cgcgggcctt catggcgtcc tcgggcgtgt ccgcgttggc
     7201 gcggacgccg agtcggcgga gatcgtcggc ccagcgcaga atggtggaga agttctcgtc
     7261 gatggacggc gagacgaggg gaacgctgcc gacgatcacg ctgccgctgg tgccgtcgat
     7321 cgtgatgatg tcgccctcgc ggagctcgga gtcaccgatc tgcaccgttc cggctgcggc
     7381 atcgatcgag agcgcctcgc agcccgcgac gcacggcttt cccataccgc gggccacgac
     7441 tgcggcgtgc gaggtcattc ccccgtgcgc cgtgacgatg ccacgcgcct cgatcagtcc
     7501 gtggatgtcg tccggcatcg tctcccagcg tacgagaatg accgactcgc cgagcttgcc
     7561 gtgctcctcg gccgtgtccg cgtcgaggac gatggcgccc gaggcggcgc cgggggaggc
     7621 gttgaggccc ttcgcggcga cctcgatcgt cgcgctcggg tcgatcatcg gatgcagcag
     7681 ctgatcgagc tgctccggat cgatccgggc gacggcgtcc tcacgcgaga taaggccctc
     7741 gtcgaccatc tcgacggcga tccggagcgc ggccgccgcc gtgcgcttgc cggtgcgggt
     7801 ctggaggagg tagagggtgc cctcctcgac cgtgaactcg atgtcctgga cgtcgcgaaa
     7861 atgggcctcg aggcgatcga gcgtgtcgac gagctccgcg tacgcctcgg gcagctcctc
     7921 ggccattcgc tcgatcggct cgggcgtgcg tatgccggcg acgacgtcct ccccctgcgc
     7981 gttgcgcaag aactcgccgt agagccgctt ctctccgttc gccgggtcgc gcgtgaagca
     8041 gaccccggtc gcggagcggt ctcccttgtt cccgaacacc atctcgacga cgttgcaggc
     8101 ggtgccgaga tcgtctggga tcgcgtaggt gcggcggtag acctgggccc gatgtgtgtt
     8161 ccaggactcg aagaccgccc tgtaggcccg gcggagctgc tcgcgcggat cctgcgggaa
     8221 gtcgtggccc gcatcctcgg cgtagatcgc gagaaacgtc ttgaccaagt cctcgaggtc
     8281 ctccgcggtg aggtcgacgt cctggctcac tccgcgatca gccttcagct cggcgagtgc
     8341 tcgctcgaag cgcgttccgg ccactccctc cacgacgctt ccgtacatct gcacgaggcg
     8401 acggtaggag tccagggcga atcgcgcgtt tccggtcgcc gccgtctgtc cggcgaccga
     8461 ggtgtcgttg aggccgagat tgaggatggt gtccatcatc ccgggcatcg agatcgacgc
     8521 tcccgaacgc acagagacga gcagcgggtc gctgtcgtca ccgaagcgct tgccagactt
     8581 ctcctcgagc gccgcgacat gggcgtcgac ctcggcatcg agcccgtcgg gaacggtgcc
     8641 gccgttggcg aggtacgcgc ggcaagcgtc ggtcgtgatc gtgaaaccgg acggaacggg
     8701 cacgcccagc gcggtcatct ccgccagccc ggcccccttt ccgccgagca aatccctgtc
     8761 tgcgccggcg tcctcctcga acgagtagac gtacttcatc cgcacgatca aagcacaacg
     8821 gggcgccggc ggtctcgtgg cgtgtcacgc atcggctgcg gagaatccac atggcgctct
     8881 atgaggcgcg ggtgccggcg cggccgatca gacatggccc tgcgggccga cgttcgaggg
     8941 ggccaccatc ctcctcccca cggctcgagc gtatccgcga accacgcaca catgcgtgac
     9001 gcgcctggag cagattcgtg gctctttcgc cgccgccggg ccggcgctgt ggtagccggc
     9061 agaccctgcc acgatgtcca gatgagccgt cgcgccttcc cacactcggt gcgcacgacg
     9121 accccgcgcc gagtcgtcct cgtctcctcc gccagcggca acgggaagac gacgatcgga
     9181 cgcctgctcg cccgccggct cggggtgccg ttccacgagc tcgacgctct cgtccacggg
     9241 cccaactggc tcgagacgcc ggacaacgag ctcagagttc tcgtcgggcg gatcgtggcg
     9301 acggacgcat gggtggtcga cggctcctac caacgcaagc tcggcgacct cgtgctcgag
     9361 cacgccgatc tcgtggtctg gctcgatctc ccgatccggg tctggctccc cagactcatg
     9421 cgacggaccg tacgacgcac gcgccggcgc gaggtgctct ggaacggcaa ccgagagacg
     9481 tacaggcaca ccgtcgtcgg ctgggactca ctcttcgtgt acgccctgcg cgcacacttc
     9541 cggcgccgcc gcgagtaccc ggtccggctg gcggcctatc cgactgtccg gcttcgctcg
     9601 agggacgcgg tcgagcgctg gctcagcctc gtcgccgggc tctgaactcg gcgacccagg
     9661 cagcgacgcg aaggccgagc cagacgggcg tgagaagcac gtagaagaca aggacgccga
     9721 ggacgatctg caccgaccac aaggcccaga agcgtgccgg cgcccacccc ttctccgtca
     9781 tgtcgcgttc caccccgcgg gcgagcgtcc cgaaccgctc gttcccctcg ctcatgcgac
     9841 ttcgctttcc cgacgccgcc tgtcgatgag acggatgacc tgcatggcgg tgccctcgac
     9901 ggaaaggtcg gagacatcga cgaccgggca gtgaagccgg cggtgcaccg cggccgcgag
     9961 ctcgagctcc tcgaagatct cgctcatctc cgcgtactga cggggtgcgc tcatgctccg
    10021 tacacgggca cggcggaact cggagagtct gtcggggtcg atcgtgagtc cgacgatctt
    10081 cagcgggtcg atctcgaaca gcgcgggcgg cggctcgact cccttcacga tcggcacgtt
    10141 cgccgtcttg tgcccgaggt agccgaggta catcgagacc ggcgtctttg agctccgcga
    10201 gacgcccaca agcacgatct cggcctcgtc gaggctgccg ccgaggccgt cgtcgtaccg
    10261 cacggcgaac tccagcgcct cgatccgccg gaaataggtg tcgtccaagg gcgggcgcgc
    10321 gcccggttcc atgcttgccg caaccccggc cacgcgggag atggagtcga tcgggtgccc
    10381 gaggaggtcg cagtagtgca ggcgcgcgct cctgcacagc cgccgcatct cctcacggag
    10441 cttcggctcg acgagggtgt acaccatgac ggccggccgc cccttcgcct gcgccacggc
    10501 gcgctggaga tctgcggccg tctcgcaccg aggatggcgg acctccgcga agggctgctc
    10561 ggggaactgg gcctcgagtg ccaacacgag tcgagcagca gtctccccgg tcgaatccga
    10621 gacgatgtgc agctcgacgg cctgccgcgc cgcgcgatcc tccgttgcca tttcgggagg
    10681 ctacccggcc gcggcgcacg ggttggctag agggggattt gcgcgaggtc gccggctcga
    10741 ccgacggcgt cgcggaggtc gcgcaggagg cgcagccggt tggcgcgcac cgccgggtcg
    10801 ggatccatga ccagaacttc gtcgaagaac cgggcgacga gcggagcggt gccggtcgcc
    10861 catgtcatga tcgcgtcgtt cccaacctcc ggcgcagcag caaccgcctc cgccagctcg
    10921 cgctccgcct gctcctcggc gagcgcacga tcgtacggag ccccgtcggc ggcgtcgccc
    10981 gcgatccggg cggcacgcgt gtagacctcg tgagccgtcg cgagctggtc cgacggaagg
    11041 gcgtggagaa actcggcgag acgggcgacg tcacccaact cgtcgcgtcc ggcgctccgc
    11101 gcggcccgca cgtactccac gggtacgtcg agcagggctt cgagtctctc ctggacgaag
    11161 tcgcgcacct cgggatcgct gatctcgatc ggaattccac cctcgatcgc cagcctgcag
    11221 agaccgatgg cggcccgccg caacgcgtag ggatcgcgtg atccggtcgg cttgtgtccg
    11281 agcgagaacg cgacgttcag cgtgtcgagc ttgtcggccg ccgccagcac acggccggcc
    11341 gccgtggacg gcagagggcc accagcagcg tccggcaggt attgctcttc gatcgcggcg
    11401 acgacgcctt cctgctttcc agcaaggcgc gcgtagacgg caccgacgtg tccctcgaga
    11461 tctgggaact cgcgaaccag ctcagaggcc tggtcggcct tggcaagtcg cgcggcctcc
    11521 acggcttctt cgtccccacc cagactgcga accaggggaa gcagccgctc cgtcttgtcg
    11581 gcgtacgagc cggccccgtg gaagaacgtg atcacaccga gacgttgcgc gagcacctcg
    11641 acgccgacgg cagcgtctcg ctcgaaggtg aatgcggcat ctgccagtcg gccctcgagc
    11701 acttgctcgt tcccggcccg cacgacgtgg ggatcgccgc cgttcgcgac gaacgcgaac
    11761 cgcgtcccgc ccagcgggaa gtaccgctga tgcgactgca ttgccgtctc gacgacccgc
    11821 tccggcagct ccaggaaacg ctcctcgaac ttcccctcga gcacgaccgg ccattcgacc
    11881 aggttgacga cctcgtcgag cttccccatc gggtcgcgcc agtcgcccag ccgatcgagc
    11941 ccctccacga tcgccgcccg gcgtcgctcc aaggagggct cgacgccgtg cgagcggaga
    12001 cggtcggcgt actcgtcggc gtcctggatc tcgaccggcc cgcttcggaa tcgatggccc
    12061 tgcgaggtcg cccccagcag cgtctgctcg tccagcttcg cgagcgtcca gcgcagcggg
    12121 cgggggaagc ggcggccgcc ttgctcccac gtcatcgtct tgccgaacgt caagccgtcg
    12181 atcacggcag cgagtcggcc cggcagagtc tctgcgatcg gctccgtcgg cttcaaccag
    12241 ccgagcacgc cgtcccgctc gacgagatcc tcgcgcgcaa ccccgagctt ccgcgcgaag
    12301 cctgcggcgg ccttctctcc gaccttcacg ggcggcccct tgatccactg ctcgggaatc
    12361 tgatcgggaa ggtcgcgcac gaggaacgcg agacgtcgtg gtccgagaaa cagctccgac
    12421 ggctcgcagt ccaggtgctc gcgcaccagc ctcggcagct gctcgcccgc ctcgtagacg
    12481 gccacagccg gcagctcctc gcagccgatc tcaagcagca gcgtcgccat ccgcctcctt
    12541 cgcaacatac gcctggcaca cagcccgagc gagcttccgc acgcggccga tgtaagcggc
    12601 ccgctcggtg accgagatcg ccccgcgcgc atccaacagg ttgaaggcgt gtgacgcctt
    12661 cagcacctgg tcgtacgccg gcaccggcag ccccgagtcg agcagctgcc tgcattcggc
    12721 ttcgtgctcg acgaaccgcc ccatcaacgt gtcgacggga gcgtgctcga agttgtacgc
    12781 agaccactgc cgctcgttct cgcggtagac gtcgccccag gtgacgccgt cgacccactc
    12841 gacctcgaat gcgctccgct tcccctggat gaacatcgcc aggcgctcga gcccgtacgt
    12901 gatctcggcg gggatcagct cgaggtcaag gcctccgacc tgctggaagt aggtgaactg
    12961 agtcacctcc atgccgtcga tccacacctc ccacccgagt ccccaggcac cgagcgtcgg
    13021 gccctcccag tcgtcctcga cgaggcggac gtcgtgccgt gtcagatcga tgccgatcgc
    13081 ctccagtgag ccgaaataga gatcgagcac gtcctcgggc gcaggcttga gaatcacctg
    13141 accctggaag tagtgctgga agcggtacgg gttctcgccg taccggccgt cggtcggtcg
    13201 gatcgacggc tgcacgtaga acgttcgcca cggacgcggg cccaggcacc ggagcaccgt
    13261 cgccggactg aacgtccccg ctccgacctc cgtgtggtac ggctgcagga tcacgcagcc
    13321 tcgcccgccc cagtacgtct gcaggccggc gatcatgtct tggaagttga ggggcatccc
    13381 ggctcgccat gctactcgcg gcgtccgaag ccgctcgccg tgccctcgtg tcgacgtggt
    13441 gacgtagccc agatcgcgcc tgctacggtc gccacagatg cgcatcgccg ttctgctggc
    13501 cgttgccgtg ccgctcacga ccgccgatct cgtctcgaag gcgtccgggt cgattccgcc
    13561 gtgggcctac catgaccggt cgttcgcgtg gatcgtgctc tgctgcgggc tgctcgtggc
    13621 cctggtgctg ataacgcgcg ttccgtcgcc gctcgtgccg cccgccgccg gcctcctggc
    13681 cgccggggtg ctcgggaacg ctctgtcggc ggcctggaac ggcctcagcg tcccgaatcc
    13741 gatcatcgtc cgcggagacg gcgccgtgct cgccttcaac ctcgcggacg tctgggtgct
    13801 ggtcgggatc atctcactcg tctcggttct ggccacctgg ctcgtgcgca acaggagcga
    13861 gcttcccgag cgtctccccg tctgggcgcg gcgccgcgcc ggctagcggg tcagacggag
    13921 gctgtccgca gccggaagcc gccgtgatac tcgtacgacg cagcaaccac cgccagggag
    13981 tcgcgcgctg cccgctcgcc gagtccgctc tcctcggcgt cggcgatggg cgtatgaagg
    14041 agcgtgcgga tgcccgcgag ccccgccggc gaaagagcga ttccgccggg gtcacacgcc
    14101 gagcacacga cgccgccggc cgccggtagg tacgcgacga gcccgctctc ggcgccgcat
    14161 tcgacgcagt cgtcgagatg tggcaggtac ccggacagcc agagcagctt cagctgaaac
    14221 gagaggacga ccgagtcgag ag
//
LOCUS       DBNGNK010000081         8907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1374379,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000081 DBNGNK010000000
VERSION     DBNGNK010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8907
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1374379"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="AC150V_02100"
     CDS             complement(<1..577)
                     /locus_tag="AC150V_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761744.1"
                     /translation="MFRKRSEEDLAADLESELDAARRQRELAAQEAATLKRELAERVR
                     AVQEKEAELDRALAEGGGLRVSDPKAKAQADALDNRLAEVERRERAVTEREQAVAAAA
                     AATAAGTAAGVDDEDTMERALDLKERTALLDEREKMIDSRQSELANRGAALDEREASL
                     AAREEALAERDKATPDGTAAPGAPDAERLEQI"
     gene            complement(653..895)
                     /locus_tag="AC150V_02105"
     CDS             complement(653..895)
                     /locus_tag="AC150V_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761745.1"
                     /translation="MNTIDLIHAEIDALSERRIELWNRLSAGLDPDVVAEIGDVEAQL
                     DELWAAHRAERARLRFGEREDIVRRARAEERLERAA"
     gene            complement(978..1304)
                     /locus_tag="AC150V_02110"
     CDS             complement(978..1304)
                     /locus_tag="AC150V_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR3761746.1"
                     /translation="MDFEEHVRNALDSLPPKVAEALKNVAVVVEDENPDDPDLYGFYD
                     GTPLPERGDMSGQLPDKISIYRLPLEEDFPDPQDLEHEIRVTVLHELGHYFGLDEGRI
                     AELGYE"
     gene            complement(1304..2239)
                     /gene="argF"
                     /locus_tag="AC150V_02115"
     CDS             complement(1304..2239)
                     /gene="argF"
                     /locus_tag="AC150V_02115"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3761747.1"
                     /translation="MPETLKGRSYTRVAAWTRAELTLALDLADELKHERAVRKELRVL
                     PGRTIGLIFHKPSTRTRVGFEVGIAELGGMALYLPAADLQLARGESFRDTALVLSRFL
                     SALMIRTYDQGEVDEFAEHASIPVINGLTDEAHPLQALADAMTIRERFGDLAGVRLAY
                     LGDGNNVCNSLMRIAGRFGMDFVAATPPGYEPRAEFVAAARDDAKANGGSVQLVADPH
                     EAVRGSQVVYTDVWASMGQEDERETRLRLLAPYRLDRTLLDAAAPDAAAMHCLPAHVG
                     EEITGDVLYGPRSLVWDQAENRLHTQKAVMALVIR"
     gene            2355..2822
                     /locus_tag="AC150V_02120"
     CDS             2355..2822
                     /locus_tag="AC150V_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3761748.1"
                     /translation="MATLVRWEPFRDLASFQTEMGRLMNGLFEGPGRATQSWVPPVDV
                     WETDEALVYAFDLPGIPEENISLEVADDTLTITAEREKTAEESGDRFYRFERRYGTFS
                     RAVGLPQGVDEGKITAAYKDGVLEVQVPKPAEQKPHKIQLGAAKADIEGTSKK"
     gene            2896..3423
                     /locus_tag="AC150V_02125"
     CDS             2896..3423
                     /locus_tag="AC150V_02125"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR3761749.1"
                     /translation="MSAFADAVNKQIAYEFGASQQYVSIAVYYDAQTLPQLAAHFYQQ
                     SVEERNHAMMMVQYLLDSNESVAVPGVEAPQTEFADVVAPVALALEQEKRVTDQISAL
                     VSLAREEGDLVGEQFLHWFLQEQREEVSSMSALLDVVERSRDNVMLVEEFLARESGGE
                     KALEQGAPPAAGGAL"
     gene            3465..3863
                     /locus_tag="AC150V_02130"
     CDS             3465..3863
                     /locus_tag="AC150V_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3761750.1"
                     /translation="MRELTVTTERQSQLIDITRDVAAAIDGENGSAALVYVPHTTAGV
                     TINEHADPAVGTDFESALERIVPEEWDWKHIEEGEENAPTHIRAALMGPSVLIPLRGD
                     GSLALGTWQGVFFCEFDGPRSRKVYVSVLR"
     gene            4016..5395
                     /gene="rlmD"
                     /locus_tag="AC150V_02135"
     CDS             4016..5395
                     /gene="rlmD"
                     /locus_tag="AC150V_02135"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR3761751.1"
                     /translation="MPPVKKGQELELDVDSLAYGGNGVARHNGFVVFVRRGLPGDRVR
                     ARVTKVKRGFAEATAEEILTPGAERVDAPCRHFGVCGGCRFQDYAYDAQVAAKQTQVY
                     DALVRLGGFADPPLEEIVPAASQFHYRNKLEYSFTQTPDGPALGFHRAGRWDEVLAVD
                     ECLLTTELGNAIRAAVRDWAREERLEPYDQVDGTGYLRHLVVREGRNTGQALVQLVTA
                     PGERFETGYFVEVLRRFDEVKSVQWSINDTPAEVTNLPSTLLWGEDAIEEEMLGLRFR
                     LRPNAFLQTNTEMAERLYALAREFAGLTGKETVYDLYCGTGTIGLALAGSALTVWGVE
                     ISEESVACAIENTELNGIGNAAFFAGNVAQSLEELQERSGQPDVVVVDPPRAGLAGKA
                     LRRTGQLQAGRIVYVSCNPTTLASDLKVLRDEYGYTLRRVRPVDMFPHTPHIESVSLL
                     HLEGSPPLG"
     gene            complement(5392..6030)
                     /locus_tag="AC150V_02140"
     CDS             complement(5392..6030)
                     /locus_tag="AC150V_02140"
                     /inference="COORDINATES: protein motif:HMM:NF016163.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HIR3761752.1"
                     /translation="MVAGVDGFPKGWIAVLVDDEGFADARIFPDAASLVAGLPDAEVI
                     CVDMPIGLPHQGTRRADEHARALVGSSLFMMFPRDVILADTHADAADRAREQTGKGIS
                     MQAYNLRAKVLDLELHLDSRMAETHPELAFLAMKGTRPPSKHTWNGLNERRLLLAREG
                     IELPHPLASGDEVPPEDVVDAAAAAWSARRVAAGDYIVLPPDPADGEPRIVF"
     gene            complement(6014..6742)
                     /locus_tag="AC150V_02145"
     CDS             complement(6014..6742)
                     /locus_tag="AC150V_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761753.1"
                     /translation="MGGASPLLSRARAAALAGLLAGLALYDVLAGELPELPDDWDVAF
                     VAGVLMPVTFATAWIALPLVRLRHLIAYALGLGIVALALFYADADGLFNSVKLLGLIL
                     LGFWFLELFQFLSWVVLIAVLIPAVDSISVWRGPTSYVVSEQPGLFDHISVAFRIPGE
                     SGSANIGPPDVLFFALFLAAADRFGLRIGWTWAAMAGLLSLTLALAAWFDVSGLPALP
                     AVCVGFLAPNIDLIWRRLRRGRWR"
     gene            complement(6718..7308)
                     /locus_tag="AC150V_02150"
     CDS             complement(6718..7308)
                     /locus_tag="AC150V_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR3761754.1"
                     /translation="MTAVPTDEIYDRYLKKAIAEINALGDDLAGLSDELHVPVLGSGH
                     PLADVFLLKHAPKPSEIQEGVAFYGRAGQALLKSLQRLGVDPLAVYGTNCLKFGTEDA
                     VEAVGWLVRELHIVQPKLLVVMGRPAVAFVNELDFPLANPIDGDSEGSIQRFTPTIAG
                     LVTPDIDESLDDQDAKTRFWNAFKALGPWWAELPPY"
     gene            complement(7351..>8907)
                     /locus_tag="AC150V_02155"
     CDS             complement(7351..>8907)
                     /locus_tag="AC150V_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HIR3761755.1"
                     /translation="LEGEEFVAIGTAVPVSRTSGIGVPVAMRANLATALRQGFDVVHA
                     FEPALPSLPYLALLEARTLTAATFLSPERLAYPPGKRQRERLLGRTDALLATSQETAE
                     AATARFPGDFRIVPLGVDLARFRPGTKRPMIVAETTSESRTRTRAALRALRDVEDWEL
                     TLLKTARLAARPTIPRAVRDRVSVRTLRRPEARSPFLAEASIFVPAPGGSGRLRLEAL
                     ASGCAITDPPGADDQPELVSAAIARLTENEAELCRLGELSRKEAAGQSFDLLAEQLES
                     VYTGISGRRRGSRDAADPLGDRDWIIADLHMHTRWSHDCSIEPEALVDHAESRGVGAI
                     AVTDHNVFGGALETVELAADRDLIVIPGEEMKTDTQGEVIGLFLREEIPQGLSFADTI
                     AAIKEQDAIVYVPHPFDRMHAIPAPETLHRNLGRIDVLETYNARLLFEAYNDEALRFA
                     RKYGLLQGAGSDAHVLQGVGTGALRMRRFRDPEEFLVSLHSAEVLRRPKSLAYLQSLK
                     WVAQVKEKTR"
BASE COUNT         1417 a         3048 c         3034 g         1408 t
ORIGIN      
        1 cgatctgctc cagccgctcc gcgtcgggcg cgccgggagc ggcggtcccg tcgggcgtgg
       61 ccttgtcgcg ctcggccagg gcttcctcgc gtgcagcgag gctcgcctcc cgctcgtcga
      121 gcgccgcgcc gcggttcgcc agctcgctct gccgtgagtc gatcatcttc tcccgctcat
      181 cgagcagggc cgtgcgctcc ttcaggtcga gcgcccgctc catcgtgtct tcgtcgtcga
      241 cgccggctgc cgtgccggct gcggtcgccg ccgcggcagc cgccacagcc tgctcgcgct
      301 cggtgacggc acgctcacgc cgctcgacct cagcgaggcg gttatccaga gcgtcggcct
      361 gcgccttcgc cttcggatcg gaaacgcgca agccgccgcc ctcggcaagc gcgcggtcga
      421 gctcggcttc cttctcctga accgctcgca cccgctcggc aagctcgcgc ttcagcgtcg
      481 cggcttcctg ggccgcgagc tcacgctggc gacgcgcggc gtcgagctcc gactcgagat
      541 cggccgctag atcctcttcg ctgcgcttgc gaaacacggg acagcgccat cttagggcga
      601 aggcgcggct gtcctgccgc gccttcgggg gttcgaaagg agtgctaccg gcctatgccg
      661 cgcgttccag gcgttcttcg gcgcgggccc ggcggacgat gtcctctcgc tcgccgaacc
      721 ggagacgtgc gcgctctgcg cggtgtgcgg cccacagctc gtcgagttgc gcctcgacgt
      781 cgccgatctc ggcaaccaca tccgggtcga gccctgcaga aagacggttc cagagctcga
      841 tacgacgctc gctcagggcg tcgatttcag cgtggataag gtcgatcgtg ttcatgtctc
      901 cggaaacgca gggtagaggc tcgacattcc ccgtccaaga gtcgcaacag aatcacaacg
      961 attccgtttt cggaccttta ctcgtagccc agctcggcga tccgcccctc gtccagtccg
     1021 aagtagtggc ccagctcgtg gagcaccgtc acgcggatct cgtgctcgag gtcttgcggg
     1081 tcggggaagt cctcttccag gggaaggcgg tagatcgaga tcttgtccgg cagctgacct
     1141 gacatgtcac cgcgctcggg caggggcgtg ccgtcgtaga agccgtagag gtctggatcg
     1201 tccgggttct cgtcctcgac gaccaccgcc acgttcttca gcgcctccgc gaccttgggc
     1261 ggcagcgagt cgagtgcgtt gcgaacgtgc tcctcgaagt ccatcagcgg atcacgagcg
     1321 ccatgacggc cttctgcgtg tgcaggcggt tctcggcctg atcccagacg agcgaccgcg
     1381 gcccgtagag cacgtccccc gtgatctcct cgcccacgtg tgccggcagg cagtgcatcg
     1441 cggccgcgtc gggagcggcg gcgtcgagca gcgtccggtc gagccggtag ggcgcgagga
     1501 ggcgcagcct ggtctcgcgc tcgtcctcct ggcccatgct ggcccagacg tccgtgtaga
     1561 caacctgcga tccacgtacg gcctcgtgcg ggtctgcgac cagctggacg ctgccgccgt
     1621 tcgccttcgc gtcgtcgcgc gccgcggcga cgaactcggc gcgtggctcg taccccgggg
     1681 gcgtcgcggc aacgaagtcc atgccgaacc ggcctgcgat ccgcatcagc gagttgcaga
     1741 cgttgttgcc gtcgccgagg tatgcgaggc gtacgccggc gaggtcgccg aaccgctcgc
     1801 ggatcgtcat cgcgtcggcc agcgcctgga gcgggtgggc ctcgtcggtg aggccgttga
     1861 tgaccggaat cgacgcgtgc tcggcgaact cgtccacctc gccctggtcg taggtgcgga
     1921 tcatcagggc ggagagaaag cgcgacagga cgagcgccgt gtcgcggaag ctctcgccgc
     1981 gtgccagctg gaggtcggcc gccgggagat agagggccat gcccccgagc tcggcgatgc
     2041 cgacctcgaa accgacccgc gtccgtgtgg acggcttgtg gaagatcagc ccgatcgtcc
     2101 gtcccggcag cacgcgcagc tcctttcgta ctgcgcgttc gtgcttcagc tcgtcggcga
     2161 ggtcgagcgc aagcgtgagc tccgcacgtg tccacgccgc aacccgcgtg tagctgcgcc
     2221 ccttgagtgt ctccggaatc cctgccacgg gcgcgatgct acccgtaaac aggtcttgct
     2281 atgatcgcac tcaagttata tactggctat agttatagac aggccgcaag gcacgtaaca
     2341 aggaggaatg ccacatggca acattggttc gctgggagcc gttccgcgat ctcgcgtcgt
     2401 tccagaccga gatgggccgc ctcatgaacg ggctcttcga gggccccggg cgggccaccc
     2461 agagttgggt gccgccggtc gacgtctggg agacggacga ggcgctcgtg tacgccttcg
     2521 acctgccggg gatccccgag gagaacatct ccctcgaggt cgccgacgac acgctgacga
     2581 tcacggccga gcgggagaag accgccgagg agtcaggcga tcgcttctat cgcttcgagc
     2641 ggcgttacgg cacgttcagc cgcgccgtcg gcctgcccca gggcgtcgac gagggcaaga
     2701 tcacggccgc ctacaaagac ggcgtgctcg aggttcaggt gccgaagccg gcggagcaga
     2761 agccacacaa gatccagctc ggcgctgcca aggccgacat cgaaggcacg tcgaagaagt
     2821 agcggggaac actccctttc cacgtctccg ggggcggtct gccggccgcc cccggcgctt
     2881 ctgtagagtc gcaccatgtc cgcgttcgcc gacgccgtca acaagcagat cgcgtacgag
     2941 ttcggggcct cccagcagta cgtctcgatc gccgtctact acgacgcgca gacgctgccg
     3001 cagctcgcgg cgcacttcta ccagcagtcg gtcgaggagc ggaaccacgc gatgatgatg
     3061 gtgcagtacc tcctcgactc gaatgagtcc gtcgccgtgc cgggggtgga ggcaccccag
     3121 accgagttcg cggacgtcgt cgcgccggtc gcgttggcgc tcgagcagga gaagcgggtc
     3181 accgaccaga tctccgcgct agtctctctc gcccgcgagg agggcgatct cgtcggcgag
     3241 cagttcctgc actggttcct gcaggaacag cgcgaggagg tctcctccat gtccgcgctg
     3301 ctcgacgtcg tcgagcgctc gcgggacaac gtgatgctcg tcgaggagtt cctcgcgcgc
     3361 gagtcgggcg gcgagaaggc cctcgagcag ggggcaccgc cggcagccgg cggcgcactg
     3421 tagtcggccc ggcgtcgtcg cccacgactg taggattgcc gcccgtgcgc gaactcaccg
     3481 ttacgacgga acgccagagc cagctcatcg acatcacccg cgacgtggcc gcggcaatcg
     3541 acggcgagaa cggctcggcc gcgctggtct acgttccgca cacgactgcc ggcgtgacca
     3601 tcaacgagca cgccgacccg gcggttggca ccgacttcga gtcggccctc gagcgcatcg
     3661 tccccgagga gtgggactgg aagcacatcg aggagggcga ggagaacgct ccaacccaca
     3721 tccgcgcggc gctgatgggg ccgagcgtgc tgatcccgtt gcgtggcgac ggctcgctgg
     3781 cgctcggcac gtggcagggc gtcttcttct gcgagttcga cggcccgcgc tcccgcaagg
     3841 tctacgtctc cgtgctccgg tagcggctct ggccggcggc gcccatccgc tagcgacgca
     3901 gtttcgccgc gcactcgtgc ggaagtcgta acagcggtcc tcagaggtcc gtcgtggccg
     3961 cttcggccac gaccgggggg gtgcgtggtg caggggcggc ggtacgctcc ctgcgatgcc
     4021 gcccgtcaag aaaggtcagg agctcgagct cgacgtcgac tcgctcgcgt acggcggcaa
     4081 cggtgtcgcg cgacacaacg gcttcgtcgt cttcgtccgg cgcggcttgc ccggcgaccg
     4141 cgtgcgtgcc cgcgtcacga aggtcaagcg cgggtttgcc gaggcgaccg ccgaggagat
     4201 cttgacgccc ggcgcggaac gtgtcgacgc gccgtgccgc cacttcggcg tctgcggcgg
     4261 gtgccgcttc caggactacg cctacgacgc tcaagtcgcc gcgaagcaaa cgcaggtcta
     4321 cgacgcgctc gtgcgtctcg gcggcttcgc cgacccaccg ctcgaggaga tcgtcccggc
     4381 ggcctctcag ttccactacc gcaacaagct cgagtactcg ttcacgcaga cacctgacgg
     4441 gccggcgctc ggctttcacc gggccggtcg ctgggacgag gtactggcgg tcgacgagtg
     4501 cctcctgacg acggagctcg gaaacgcgat ccgcgctgcc gtccgcgact gggcgcgcga
     4561 ggagcggctc gagccgtacg accaggtgga cggcaccggc tatctccgac acctcgtcgt
     4621 gcgcgagggg cggaacacgg ggcaggcgct cgtccagctc gtcaccgcac caggcgagcg
     4681 cttcgagacc ggctatttcg tcgaggtgct gcgccgtttc gacgaagtca agtccgtgca
     4741 gtggtcgatc aacgacacgc ctgccgaggt gacgaatctg ccgagcacgc tcctctgggg
     4801 tgaagacgca atcgaagagg agatgctcgg gctgcggttc cgtctgcggc ccaacgcttt
     4861 tctccagacg aacaccgaga tggcggagcg tctgtacgcg ctggcacgcg agttcgccgg
     4921 actgaccggc aaggaaacgg tgtacgacct ctactgcggc actgggacga tcggcctcgc
     4981 gctggccgga agcgctctga ccgtttgggg cgttgagatc tcggaggagt cggtcgcctg
     5041 cgcaatcgag aacaccgagc tgaacggcat cggcaacgcc gcattcttcg cgggcaatgt
     5101 cgcgcagtcg ctcgaggagc tccaagagcg ctccggccag cccgatgtcg tcgtcgtcga
     5161 cccgccacgc gccgggctcg ccggcaaggc gctgcggcga accggacagc tacaggccgg
     5221 ccggatcgtc tacgtctcgt gcaacccgac gacgctcgcc tccgacctga aggtgctccg
     5281 tgacgaatac ggctacacgc tgcgccgcgt ccgcccggtc gacatgttcc cgcacacgcc
     5341 tcacatcgag agcgtgtcct tgctgcacct cgaaggcagc ccgcctctcg gctagaacac
     5401 gatccggggc tcgccgtcgg cggggtcggg cgggaggacg atgtagtcgc cggcagccac
     5461 ccgccgggcg ctccaggccg cggcggctgc gtcgacgacg tcctcgggcg gaacctcgtc
     5521 gccgctcgcc agcgggtgcg ggagctcgat tccctctctc gccagcagca gtcgccgctc
     5581 gttgagcccg ttccacgtgt gcttggaggg tgggcgggtt cccttcatcg cgaggaaggc
     5641 gagctcggga tgcgtctcgg ccatccggga gtcgagatgg agctcgagat cgagaacctt
     5701 cgcccgcagg ttgtacgcct gcatgctgat cccctttccc gtctgctcgc gcgcgcggtc
     5761 ggccgcatcc gcgtgcgtgt cggccaggat cacgtcgcgc ggaaacatca tgaagaggct
     5821 ggaaccgacg agagcacggg cgtgctcgtc ggcgcgccga gtgccctggt ggggcagacc
     5881 gatcggcatg tccacacaga tcacctccgc gtcgggcagc ccggccacga gcgacgcggc
     5941 gtcgggaaag atcctggcgt ccgcgaaacc ctcgtcgtcg acgagcaccg cgatccagcc
     6001 cttggggaag ccgtcaacgc cagcgaccac ggcgcagcct tctccagatc agatcgatgt
     6061 tcggtgcgag gaagccgacg cagacggcgg gcagcgcggg gaggccggag acgtcgaacc
     6121 aggcggccag agccagggtc agggatagca ggccggccat cgcggcccag gtccagccga
     6181 tacgcagccc gaagcggtcg gctgctgcaa ggaagagggc gaagaagagc acgtcgggag
     6241 gcccgatatt ggccgacccg ctctctccgg gtatgcggaa cgcaaccgag atgtggtcga
     6301 agaggcccgg ctgctcgctc acgacgtagc tcgtcggccc acgccagacg gagatcgagt
     6361 cgacggcggg gatcagaacg gcaatcagca ccacccacga gaggaactgg aagagctcga
     6421 gaaaccagaa ccccaggagg atcaggccga gcagcttgac gctgttgaag aggccgtcgg
     6481 cgtcggcgta gaagagcgcc agcgcgacga ttccgagtcc gagcgcgtag gcgatgagat
     6541 gccgcaaccg gacgagcggc agggcaatcc acgcggtcgc gaacgtcacc ggcatcagga
     6601 cgcccgcgac gaaggcgacg tcccagtcgt ccggcagctc gggcagctcg cccgcgagca
     6661 cgtcgtacag ggcaagcccg gcgagcagcc cggcgagcgc ggccgctcgc gcgcggctca
     6721 gtagggggga agctccgccc accaggggcc gagcgccttg aacgcgttcc aaaagcgggt
     6781 cttcgcgtcc tggtcgtcga gcgactcgtc gatgtccggc gtcacaagac ctgcgatcgt
     6841 cggcgtgaaa cgctggatcg agccctcact gtctccgtcg atcggattgg ccagcgggaa
     6901 gtcgagctcg ttcacgaacg ccactgccgg tcgtcccatc acgacgagca gcttcggctg
     6961 cacgatgtgc agctcgcgga cgagccagcc gacagcttcc accgcgtcct ccgttccgaa
     7021 cttcaagcag ttcgtcccgt acacggcgag ggggtcgaca cccaggcgct gcagcgactt
     7081 cagcaacgcc tggccggctc tcccgtagaa agcgacgccc tcctggatct cggaaggctt
     7141 cggcgcgtgc ttcaggagga agacgtcggc gagcgggtgg ccggagccga gcacggggac
     7201 gtggagctcg tcggacaagc ccgccagatc gtcgccgaga gcattgatct cggcgatcgc
     7261 cttcttgaga tagcggtcgt agatctcgtc ggttggcacg gcggtcacgg gagcgacctc
     7321 aacttctctc ctcgtgcggc ttctccttga ctagcgcgtc ttctccttga cctgggccac
     7381 ccacttcagc gactgcaggt acgcgagcga cttcggccgc cggagcacct ccgccgagtg
     7441 gagcgagacg aggaactctt cggggtcacg gaagcgccgc attcgcaacg cccccgtgcc
     7501 caccccctgc agaacgtggg catcggagcc ggcgccctgg agcaggccgt acttgcgtgc
     7561 gaagcggagg gcctcgtcgt tgtaggcctc gaacaggagc ctggcgttgt aggtctcgag
     7621 cacgtcgatc ctgcccagat tccggtgcag cgtctccgga gccgggatcg cgtgcatgcg
     7681 gtcgaacggg tgcggcacgt agacgatcgc gtcctgctcc ttgatcgctg cgatggtgtc
     7741 tgcgaaggag agtccctgcg gtatctcctc acggagaaac agcccgatca cctctccctg
     7801 cgtgtccgtc ttcatctcct caccgggaat gacgatcaga tcgcggtcgg cggccagctc
     7861 gacggtctcc agcgctcccc cgaacacgtt gtgatcggta acggcgatgg ctccgacccc
     7921 ccggctctcg gcgtggtcga cgagcgcctc cggctcgatc gagcaatcgt gcgaccaccg
     7981 tgtgtgcatg tgcaggtcgg cgatgatcca gtcgcggtcg ccgagcgggt ccgctgcgtc
     8041 ccgggagcct cggcgccgtc cagaaatccc cgtgtagacg ctctcgagct gttccgccag
     8101 gaggtcgaag ctctgccccg ccgcttcctt gcggctcagc tcgccgagcc ggcaaagctc
     8161 ggcctcgttc tcggtcagcc gcgctatggc tgccgagacg agctcgggct ggtcgtctgc
     8221 tcccggcgga tccgtgatcg cacagcctga ggcgagcgcc tcgagccgaa gccgcccgct
     8281 cccgccgggt gcgggaacga agatcgacgc ctccgcgagg aacggcgagc gagcctcggg
     8341 acggcgaagt gtgcggacag agaccctgtc gcggacggcg cgcgggatcg tcggtcgtgc
     8401 cgcgaggcgc gctgtcttca ggagcgtcag ctcccagtcc tccacgtctc gcagcgcccg
     8461 cagcgccgct ctcgtcctcg tgcggctctc ggacgtcgtc tcggccacga tcatcggtcg
     8521 cttcgtgccc ggacggaagc gcgcgagatc gaccccgagc ggcacgattc tgaagtcgcc
     8581 cggaaagcgt gcggttgcag cctcggccgt ctcctgcgac gtggcgagca gagcgtcagt
     8641 cctgccgagc agccgctcgc gctgccgctt cccgggcggg tatgccagac gctccgggga
     8701 aaggaaggtc gccgcggtca gagttctcgc ctcgagcagc gcgaggtagg ggagactcgg
     8761 gagcgccggc tcgaaggcgt gcacgacgtc gaagccctgc cgaagcgccg tcgcaagatt
     8821 cgcgcgcata gcgaccggaa ccccgatacc gctcgtccgc gagacgggaa ccgccgtgcc
     8881 gatcgcgacg aactcctcgc cctcgag
//
LOCUS       DBNGNK010000082         4986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_85026,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000082 DBNGNK010000000
VERSION     DBNGNK010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_85026"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1529)
                     /locus_tag="AC150V_02160"
     CDS             complement(171..1529)
                     /locus_tag="AC150V_02160"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II 3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR3761756.1"
                     /translation="MTVTEPTWTPSSWRALPASQQPDWPDAAALEDVAARLREVPPLV
                     FAGEARSLLERLGEVVDRRAFVLHAGDCAESFRDVSAIAIRERLKILLQMSAVMSYGA
                     MLPVVKLGRIAGQFAKPRSSSFEEVNGVELPSFRGHAVHSEQPTPDARRPDPERLLQA
                     YYQATSTLNILRAFTKGGFADLTRVHTWNQEFVATSPQGRRYEQIATEIDRALRFMQA
                     IGINLGAERTVQEVDVWTSHEGLLLDYEEPLTRRDSTTGDWYACSAHLLWIGERTRSL
                     DEAHVEFFSGVHNPIGVKVGPGASAHDVVALCERLNPRRIAGRLTLIARMGADSVREL
                     LPPLLAAVSEAELPVVWMCDPMHANTFRAGSGHKTRRFDDVIGEIEGFFAAHREAGTW
                     PGGVHIEFTGDDVTECLGGSTEVSEAQLDHRYETLCDPRLNARQSLDLAFRVAELMRS
                     VA"
     gene            1775..2758
                     /gene="trpS"
                     /locus_tag="AC150V_02165"
     CDS             1775..2758
                     /gene="trpS"
                     /locus_tag="AC150V_02165"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR3761757.1"
                     /translation="MRIFSGIQPTGDKHLGNYIGGFRQYASTQEQGEAFFCIVDLHSV
                     TVEYDPADLRERTLDLYAMLVATGLDPERSTVFAQSHVTAHAEASWLLSAVTSYGQLG
                     RMTQFKDKAEAREFISSGLFTYPVLMAGDILLYQTDIVPIGDDQRQHLELTRDIAERF
                     NARYGETFVVPEGVYPEVGARIMDLQDPTNKMATTGGTAQGTVRLLDEPEVIRKKFRS
                     AVTDSGREIRRGDDKPGITNLVDILAVATGKTPGQIEVEYDGEGYGRFKSDVGDAVAE
                     LLAPVRERYTELRGDRGELLRLLKVGADKARAASAPTLSAMYERMGFVPLA"
     gene            complement(2766..3461)
                     /locus_tag="AC150V_02170"
     CDS             complement(2766..3461)
                     /locus_tag="AC150V_02170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR3761758.1"
                     /translation="MRLNAYLARAGIASRRGADELIKSGRVRVNGGAAALNTFVGKDD
                     TVEVDGERAEAHRLAHVLLNKPAGVVTTASDPEGRPTVVDLVGHEVRVVPVGRLDMPT
                     TGVLLLTNDGPLAHRLAHPRYEVEKTYVATVRGEPTDAALAALAEGVELDDGPTAPAR
                     VRRVSATRIELVLHEGRNRQVRRMCEAVGHPVRTLHRTAYAGIGADSLSPGEWRELDR
                     AEVESLRRLVGLA"
     gene            3494..4696
                     /locus_tag="AC150V_02175"
     CDS             3494..4696
                     /locus_tag="AC150V_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3761759.1"
                     /translation="MQHSAELTRRLADLVVCNGANVQPGQIVGVTSYVGKERLTREVA
                     RAAYERGARWVDVLYFDQWVKRERIKHAPDETLGDVPPWMLERIEWLSAERAARITLQ
                     GPHAPRALNGLDPKRAGRDLLPYLPLTGEIVNRRTTNWCVAPGPTVDWAELVYPELSG
                     EAALAKLWETIAYICRLDADDPVAAWSARLEDLRRVAAGLDDRRFDAMRLHGPGTDVT
                     IGLLPSSMWQAAGFTTLDGIEHNPNIPTEEVFTTPDPARVDGVVTATLPLELYGSIID
                     GIRVEFEGGRAVKIDAERGADALRSAIAKEGGDRLGEVALVDGEGRIGPLETVFYETL
                     LDENAASHIALGNGYTLTVADEGDRARVNRSEIHVDFMIGSPELHVDGLAADGTTIPV
                     LRAGEWQI"
     gene            complement(4706..>4986)
                     /gene="scpB"
                     /locus_tag="AC150V_02180"
     CDS             complement(4706..>4986)
                     /gene="scpB"
                     /locus_tag="AC150V_02180"
                     /inference="COORDINATES: protein motif:HMM:NF016005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR3761760.1"
                     /translation="VAYLGPCSRPEIARIRGVAADSAVASLLDRGLVTEAGRESGLGG
                     AVRYRVTPLFERVFGLESLAALPRLDDLGADADAMRERLLAVAEARAS"
BASE COUNT          810 a         1726 c         1741 g          709 t
ORIGIN      
        1 gagcgagggc gcgggagata cagcgccctt ctctgccgcg gccgcgagga cgcccgagtc
       61 gatatcccat ccggtcacgg tctcgactcc ggcccgacgg gcaccgaggg cggcagacga
      121 gccgatcagc cccgtgccga cgacggtgag cgcacggatc ggcgcggaca tcaggcgacg
      181 ctgcgcatca gctcggcgac tctgaatgcg agatcgagcg actggcgggc gttcaggcgc
      241 gggtcgcaga gcgtctcgta gcggtgatcc aactgcgcct cggagacctc cgtcgagccg
      301 ccgaggcact cggtgacgtc gtcccccgtg aactcgatgt gcacgccgcc gggccatgtc
      361 cccgcttcgc ggtgggctgc gaagaacccc tcgatctcgc cgatcacgtc gtcgaagcgc
      421 cgcgtcttgt ggccggagcc agcgcggaac gtgttcgcgt gcatcggatc gcacatccag
      481 acgacaggca gctccgcctc gctgacggcg gcgaggagcg gcgggagaag ctcccgcacg
      541 gagtcggcgc ccatgcgtgc gatcagggtc aggcgccccg ctatgcgtcg tgggttcagg
      601 cgctcgcaca gggcgacgac gtcgtgtgca gatgcgcccg ggcccacctt gacgccgatg
      661 gggttatgca cccccgagaa gaactcgacg tgagcctcgt ccaggctccg ggtgcgctca
      721 ccgatccaga ggaggtgggc ggagcaggcg taccagtcgc ccgtagtgga gtcacggcgg
      781 gtgagcggct cctcgtagtc gagcagcagg ccctcgtggc tcgtccacac gtcgacctcc
      841 tgaacggtac gctcggcccc caggttgatc ccgatcgcct gcatgaagcg gagcgcccgg
      901 tcgatctctg tcgcgatctg ctcgtagcgg cggccctgcg gcgaggttgc gacgaactcc
      961 tggttccacg tgtggacgcg cgtcaggtcg gcgaagccac cctttgtgaa ggcacgcagg
     1021 atgttcagcg tcgacgtcgc ctggtagtag gcctgtagca ggcgctccgg atccggacgc
     1081 cgcgcgtccg gagtgggctg ctccgaatgc acggcgtgcc cgcggaagga gggaagctcg
     1141 acaccgttca cctcctcgaa ggaggacgac cgtggcttgg cgaactggcc ggcgatccga
     1201 cccagcttca cgaccggcag catggcgccg tagctcatca ccgccgacat ctgcagcagg
     1261 atcttcagcc gctcgcggat cgcgattgcc gagacgtccc gaaacgactc tgcgcagtcg
     1321 cccgcgtgga gtacgaaggc gcgcctgtcg acgacttcgc cgagacgctc gagcagagag
     1381 cgtgcctcgc cggcgaagac cagcggcggg acctcccgga gccgcgccgc gacgtcctcg
     1441 agagctgcgg catcgggcca atccggctgc tgcgaggcgg gaagcgcgcg ccacgaggac
     1501 ggcgtccagg tgggttccgt gaccgtcacg gtcgcagtgt agtgagagcc gtgttgacgt
     1561 ttggcccgcc ggcggctatc acatgccgaa tggcatgccg ctgcgcacac cggctctggc
     1621 tctggccctc gggccgcggc ggccgtctgc ccgcacgcta gcgcgcgcgg tctcgccgtc
     1681 cccgccccgg ccaccgtctt aggcccagca agcgagcaag taacagtctg ttacttgctc
     1741 acacgcgcga tcagaaaagg agagaatcga ggcaatgcgc atcttctcgg gcatccagcc
     1801 gaccggcgac aagcacctcg gcaactacat cggcggcttc cgccagtacg cgtcaacgca
     1861 ggagcagggc gaagccttct tctgcattgt cgacctgcac tcggtcacgg tcgagtacga
     1921 cccggccgac ctgcgggagc gaacactcga cctgtacgcg atgctcgtgg caaccgggct
     1981 cgaccccgaa cgctcgacgg tcttcgcgca gagtcatgtc accgcgcatg ccgaggccag
     2041 ttggctgctg tcggccgtga cgagctacgg gcagctcggc cgcatgaccc agttcaagga
     2101 caaggccgag gcgcgcgagt tcatctcctc cgggctgttc acgtacccgg tgctgatggc
     2161 cggggacatc ctgctctatc agaccgacat cgtcccgatc ggcgacgacc agcgccagca
     2221 cctcgagctc acgcgcgaca tcgcggagcg cttcaacgcg cgctacggcg agacgttcgt
     2281 cgtgccggag ggcgtgtatc cggaggtcgg cgcgcggatc atggatttgc aggatccgac
     2341 gaacaagatg gcaacgaccg gcggcaccgc acagggcacc gttcgccttc tcgacgagcc
     2401 cgaggtgatc cgcaagaagt tccggagtgc ggtgacggac tccggacgtg agatccggcg
     2461 tggcgacgac aagccgggga tcacgaacct cgtcgacatc ctcgccgtcg cgacgggcaa
     2521 gacgcccggc cagatcgagg tcgagtacga cggtgagggc tacggcaggt tcaagagcga
     2581 cgtcggcgac gccgtcgccg agcttctggc tcccgtccgg gagcgctaca ccgagctgag
     2641 aggcgaccgc ggggagctcc tgcggcttct gaaggtcggc gcggacaagg cacgcgcggc
     2701 ctcggcgccg acgttgtccg ccatgtatga gcggatgggc ttcgtgccgc tcgcgtgacg
     2761 cgaagctagg cgaggcccac gagtcggcgc agactctcca cctcggcccg atccagctct
     2821 cgccactcgc cgggcgagag gctgtccgcg ccgatacccg cgtaggcggt gcggtgcagg
     2881 gtccgcaccg ggtggccgac cgcctcgcac atgcgccgaa cctgccggtt ccggccctcg
     2941 tgcagcacga gctcgatccg agtcgcagag acccgccgaa cgcgtgcggg cgccgtcggg
     3001 ccgtcgtcga gctcgacacc ctccgccaga gcggccagcg ccgcgtcggt cggctcgccc
     3061 cggacggtcg cgacgtacgt cttctcgacc tcgtagcgtg gatgcgcgag ccggtgagcg
     3121 agaggcccgt cgttggtcag cagcagaacg ccggtcgtgg gcatgtcgag cctgccgacg
     3181 gggacgactc gcacctcgtg accaacgagg tcgaccaccg tcggccggcc ctcggggtcg
     3241 ctcgccgtcg tcacgacccc ggcaggcttg ttcaggagca catgcgcgag gcggtgagcc
     3301 tcggcgcgct cgccgtccac ctccacggtg tcgtccttcc cgacgaaggt gttcagcgcc
     3361 gcagccccac cgttcacccg gacacgaccg cttttgatca gctcgtcggc acctcgtcgt
     3421 gacgcgatgc ccgcgcgcgc gagatacgca ttcagccgca cgaccgtatt ctcgcccggg
     3481 tagggtgccg acggtgcagc actccgccga gctgacccgg cgcctggccg atctcgttgt
     3541 ctgcaacggc gcgaacgtcc agccgggcca gatcgtcggc gtgacgagct acgtcggcaa
     3601 ggaacggctc acccgcgagg tcgcgcgcgc cgcatacgag cgcggggcac gctgggtcga
     3661 cgtgctctat ttcgaccagt gggtcaagcg tgagcggatc aagcacgcgc cagacgagac
     3721 cctcggcgac gtgccgccgt ggatgctcga gcggatcgaa tggctctcgg ccgagcgggc
     3781 cgcgcggatc acgctgcagg ggccgcacgc gccgcgcgcc ctgaacgggc tcgatcccaa
     3841 acgggcgggg cgcgacctgc ttccgtacct gccactcacg ggagagatcg tcaaccggcg
     3901 aacgaccaac tggtgcgtcg cgccgggccc gacggtcgac tgggccgagc tcgtgtatcc
     3961 ggagctgtcc ggggaggcgg cgctggccaa gctgtgggag acgatcgcgt acatctgtcg
     4021 actggacgcg gacgaccccg tcgcagcctg gagcgcgcgc ctggaagacc tccgccgtgt
     4081 cgcggcgggg ctcgacgacc gccgcttcga cgcgatgcgt ctgcacgggc ccggcaccga
     4141 cgtcacgatc gggctcctgc cgtcgtccat gtggcaggcg gccggcttca ccacgctcga
     4201 cgggatcgag cacaacccga acattccgac cgaggaggtc ttcacgaccc ccgatcccgc
     4261 ccgagtcgac ggagtcgtca ccgcaacact gccgctggag ctgtacggct cgatcatcga
     4321 cgggatccgc gtcgagttcg aaggcggccg ggcggtcaag atcgacgccg agcgcggcgc
     4381 cgacgccctc cggtcggcga ttgcgaagga gggcggagac cgactcggtg aggtcgcgct
     4441 cgtcgacggc gagggccgga tcgggccgct cgagaccgtc ttctacgaga ccttgctcga
     4501 cgagaacgcc gcgagccaca tcgcgctcgg gaacgggtac acgctgaccg tcgcggacga
     4561 aggcgacagg gcgcgcgtca accgcagcga gatccacgtc gatttcatga tcggcagtcc
     4621 ggagctccac gtcgacggac tggcggccga cggcacgacg attcccgtgc tccgcgccgg
     4681 cgagtggcag atctgagggt ggccgctacg aagcgcgggc ctcggcgacg gcgagaagcc
     4741 tttcccgcat ggcgtcggcg tcggctccga ggtcgtcgag acgcggcagc gccgcaaggc
     4801 tctccagccc gaacacccgc tcgaacagcg gcgtcacgcg gtagcgaacg gctccgccca
     4861 ggccgctctc acggccggcc tcggtaacga ggccacgatc gaggagcgag gcgacggccg
     4921 agtcggccgc gacgccgcgg atgcgggcga tctcaggtcg cgagcacggc ccgaggtagg
     4981 cgacga
//
LOCUS       DBNGNK010000083         2307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_585691,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000083 DBNGNK010000000
VERSION     DBNGNK010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_585691"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="AC150V_02185"
     CDS             <1..1191
                     /locus_tag="AC150V_02185"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR3761761.1"
                     /translation="AVTLVDELYAAVDVKEEIEDQLPEDVKGLAGPLAAGSRELAERA
                     AQLLLERPRIQTLWVETLGAAHEELVLILEGREGVVSTTGGEVVLDLRELVVRLGEEV
                     GVGGTVADRIPEDAARIEVLKSDELETAQDAVSILDAVALWVWVLALAAWGLAIWLAR
                     GYRRIEVRAIAVGLLIVGISMLLVRTLAGNYLVSELVKTESAKPAAEDAWGILTRLLN
                     DQAWTVIIMGVFALAGVWFAGPSRAARSARGALAPYIKRPEIAFGVYGVLALIFVWWG
                     PTNQWRSFVTAIVMIALGALGVEAIRRVTASDFPDAVASDVWGSVRRKADEWSGALRE
                     YRPTTEGPVEAEVREPPPPPPPPTPPAAPAALADQLAALAALHAQGALSAEEFAAAKK
                     KLLG"
     gene            complement(1207..1788)
                     /locus_tag="AC150V_02190"
     CDS             complement(1207..1788)
                     /locus_tag="AC150V_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761762.1"
                     /translation="MRRFLGTGFVIALLAVAAGCGGGGDGSSGDSGSSAAAAEEWADA
                     FCTTLAGWRDDLEQIGRDIADDPTSLSRDTLENAATDARDRTEQLVDDLRDLGAPDTE
                     AGEATKDAIEELGDDLERELDEARAAVEDASGITGVLSAIGTVTSSFTAAGEAIDATY
                     DEIRELDADGELENSLRDTESCKDLRGAGDESG"
     gene            1908..>2307
                     /gene="cysC"
                     /locus_tag="AC150V_02195"
     CDS             1908..>2307
                     /gene="cysC"
                     /locus_tag="AC150V_02195"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007357317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HIR3761763.1"
                     /translation="MVSRGFVVWFTGLSGSGKTTIASGVRAELERRGVVVDILDGDVV
                     REHLSKGLGFSKEDRDVNIERIGWVASRLARAGAGVLVSAISPYEETRRHARAMVEEH
                     ALFIEVHVATPLEECARRDVKGLYEKAFRGE"
BASE COUNT          324 a          798 c          821 g          364 t
ORIGIN      
        1 gcggtcacgc tcgtcgacga gctctacgcc gcggtcgacg tgaaggagga gatcgaggat
       61 cagctgcccg aggacgtcaa gggcctggcc gggccgctcg ccgccggctc gcgtgagctg
      121 gccgagcgcg ccgcccagct cctgctcgag cggccacgca ttcagacgct gtgggtcgag
      181 acgctgggag cggcacacga ggagctggtc ctgatcctcg agggacggga aggcgtcgtc
      241 tcgacgactg gaggagaggt ggtcctcgac ctgcgcgagc tcgtcgtgcg gctcggggag
      301 gaggtcggcg tcggtggcac ggtggccgat cggatccccg aggacgctgc gcggatcgag
      361 gttctcaagt cggacgaact ggagacggcg caggacgccg tgagcatcct cgacgccgtc
      421 gcactctggg tctgggttct cgcactcgcg gcctgggggc tcgcgatctg gctcgcgcgc
      481 gggtaccggc ggatcgaggt gcgcgcgatc gccgtcggtc tgctgatcgt cggcatcagc
      541 atgctcctgg tccgaacgct tgccggcaac tacctcgtga gcgagctcgt caagaccgag
      601 tcggcgaagc ccgccgcgga ggatgcgtgg ggcatcctca cccgcctgct gaacgatcag
      661 gcctggactg tgatcatcat gggcgtcttc gcgctcgccg gcgtctggtt cgccggcccg
      721 agcagggccg cccgctcggc gcgcggagcg cttgctccct acatcaagcg acccgagatc
      781 gccttcggcg tatacggcgt cctcgccctg atcttcgtct ggtgggggcc gaccaaccag
      841 tggcggagct tcgtgacggc gatcgtgatg attgcgctcg gggcgctcgg cgtggaggcg
      901 attcgccgcg tgaccgccag cgacttcccg gacgcggtcg cgagcgacgt ctggggctcg
      961 gtccgccgca aggccgacga gtggagcggg gccctgcgcg agtaccgacc gacgacggaa
     1021 gggcccgtcg aggccgaggt ccgagagccg ccgccgccgc caccaccacc gacaccgccc
     1081 gcggcgcccg ccgcgcttgc cgaccagctc gcggcgctcg ccgcattgca cgcgcagggt
     1141 gcgctctccg ccgaggagtt cgccgccgcg aagaagaagc tgctcggcta gcccgccgtc
     1201 cggcggctag ccggactcgt cgcccgcgcc gcggaggtcc ttgcaggact cggtgtcccg
     1261 caacgagttc tccagctcgc cgtcggcgtc gagctcgcga atctcgtcgt aggtcgcgtc
     1321 gatcgcctcg ccggcggccg tgaacgacga cgtgaccgtt ccgatcgcgc tcaggacacc
     1381 cgtgatgccg ctggcgtcct cgaccgccgc ccgcgcttcg tccagctcgc gctccagatc
     1441 gtcgcccagc tcctcgatcg cgtccttcgt cgcctcgccg gcctcggtgt ccggggcgcc
     1501 gaggtcgcgc agatcgtcga cgagctgctc ggtcctgtcg cgcgcgtcgg tggcggcgtt
     1561 ctcgagcgtg tcacgcgaca gggaggtcgg gtcgtccgcg atgtcgcggc cgatctgctc
     1621 caggtcgtcc cgccagccgg cgagcgtggt gcagaaggcg tcggcccatt cctccgctgc
     1681 cgccgcgctg ctgcccgagt ctccagagga gccgtcaccc ccgccgccgc agcccgccgc
     1741 aaccgcgaga agcgcgatga cgaagccggt cccgagaaag cggcgcacgg accgactgta
     1801 gcagtggcga acaaataaga gatacttatt acaactatta ggtttcgcgc tgctatcgtg
     1861 ccgtgccgtg gcccctgcca acgtccgtct gcagtgccgt tcgccgcgtg gttagccgcg
     1921 gcttcgtcgt ctggttcacc ggtctttccg gctcgggaaa gacgactatc gcgagcggcg
     1981 tgcgcgccga gctcgagcga cgcggcgtcg tggtcgacat cctcgacggc gacgtcgtgc
     2041 gcgagcacct ctcgaagggc ttgggcttct cgaaggagga ccgcgacgtg aacatcgagc
     2101 gaatcggctg ggtcgcgtcg aggctcgccc gggcgggcgc cggcgtgctc gtctctgcga
     2161 tctcaccgta cgaggagacc cgtcgccacg cacgcgcgat ggtcgaggag cacgcgctct
     2221 tcatcgaggt gcatgtcgcg accccgctcg aggagtgcgc tcgacgagac gtgaaaggcc
     2281 tctacgagaa agccttccga ggagaga
//
LOCUS       DBNGNK010000084         4333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1733097,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000084 DBNGNK010000000
VERSION     DBNGNK010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1733097"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..2208)
                     /locus_tag="AC150V_02200"
     CDS             complement(103..2208)
                     /locus_tag="AC150V_02200"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3761764.1"
                     /translation="MFGIASVISGRRSKFVVLALWLVLVVGLGPLAGKFEQAQRNEPS
                     SFLPGDAESVAVLKAADGFPSGDVTPAIAVIRAPGELGAEGQEAVEAARQRISTAGID
                     GVQSLPPPAYSQAGDAAVLTIPIEADGDEEVLIGAVEDARELVRQGLPPQLEARVTGP
                     AGFSADASKAFEGINSTLLFATAGLVFILLVLIYRSPIFWILPLVSVLFAEAVVRGLG
                     SLLAEAGVVINGQTGGILLVLVFGAGTNYALLLTARYREELHQVEDKHEAMLIAVRQA
                     GPAILASAGTVVAALLCLSFARVNSTAGLGPVGAMGVAVAAAAMLTLLPALLLLGGRR
                     AFWPFVPRYGTEPKAERAGIWRRLGWWIERRRRPVWIATALGLAVMSLGALTLDDDLT
                     TANAFRGSVESVQGQELLEQSFPAGASAPTVVLVRDESRTDAALAAARRAPGVAAVTE
                     PERGEAGARFEVTLEADPFSKEGYAAIEPLRAELRGTAGDSVLVGGATAEEADLRQAV
                     KRDTKLLVPLVLVVVFALLVLLLRSLAAPFMLVATVLLSFFAALGGSLVLFELFADFP
                     GEDPSYALFAFIFLVALGVDYNIFLMARVREESLELPTREAMLKGLAVTGGVITSAGI
                     VLAGTFSVLAVLPLVALTQIGITVAFGVLLDTFVVRSILVPALTFDLGERTWWPSALW
                     RRRPVPARERRRLSVEEQT"
     gene            complement(2424..3644)
                     /locus_tag="AC150V_02205"
     CDS             complement(2424..3644)
                     /locus_tag="AC150V_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3761765.1"
                     /translation="MPSFAYDAINAQGFELKGEIQAADVGAARDALRGRGLMTRSIAE
                     VSGGEAGGDSKRGFFQPKVKTKSLQIFSRQFATMIEAGLSVVAALVILEQQTDDKALK
                     VVVDDVRDKVESGALLSEAMAGHPEVFSRLYIAMVEAGEAAGVLDTVLDRVATQIEKE
                     QKIRRRVKGAMMYPSVVLVFATLVMVGMLMFLVPVFVKIFDQLGGELPVLTQYVLKVS
                     NALRGYWFIIFPLIGAGVFGFVKWKKSERGRQVWDVFKLRIPMQIGTVVRKIAMARFS
                     RTLATLVASGVDIIRALEITAQTSGNWVIERETMDMRIRVQEGAPIAQPLIDSAAFPP
                     MVGQMVKIGEETGELEQMLAKVADFYEDEVDASISALTSIIEPLMMVGVGMMVGIIII
                     SMYLPMFKMMSLIQ"
     gene            complement(3686..>4333)
                     /locus_tag="AC150V_02210"
     CDS             complement(3686..>4333)
                     /locus_tag="AC150V_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761766.1"
                     /translation="GKPTSTAFVAGVVLPGGFADQGGDGRLLVFNRMPAKDPFVQQVD
                     DKPVDAGGGDGSDGTQAANASSSVPPAPSKPGTTPSEPGTGTTEPSGSGGTTTPAIPT
                     DPVKPAKPPVLTFATINLNGTPVALEVKKPFPTPQNLFVVTKLSETDAKLAIAGDGGF
                     TGGRKAVSLQMGKALTLVDQATGVRYTLKLLYTGTTPEQTAEFTAPADDQGSAGK"
BASE COUNT          765 a         1475 c         1443 g          650 t
ORIGIN      
        1 cgtgactcgc gtctcgggtg cggagctgga tcgcccagag cgcagccatg ccgacggagg
       61 cgaacgcggc cccgacgagc agcagcagga tgtacgagcc gatcacgtct gctcctcgac
      121 ggagagccga cgacgctcgc gagccggcac cggccgccgg cgccagagcg cggacggcca
      181 ccacgtgcgc tccccgagat cgaacgtcag ggcgggcacg aggatcgagc gcacgacgaa
      241 cgtatcgagc aggacgccga aagcgaccgt gatcccgatc tgggtcaacg ccacgagcgg
      301 cagcacggcg agcacggaga aggtgcccgc caggacgatg ccggcggagg tgatgacgcc
      361 gccggtcacg gcgaggccct ttagcatcgc ctcccgggtc ggcagctcca gtgactcttc
      421 gcgaactcgg gccatgagga agatgttgta gtcgacgccg agcgcgacga ggaagatgaa
      481 cgcgaagagc gcgtagctcg gatcctcccc cgggaagtcg gcgaacagct cgaagaggac
      541 gagcgacccg ccgagcgcgg cgaagaacga caggagcact gtcgcgacca gcatgaacgg
      601 cgcggcgagc gaccggagca acagcacgag cagagcgaag acgacgacga gcacgagcgg
      661 aacgaggagc ttcgtgtcgc gcttcaccgc ctggcggaga tcggcctctt ctgccgtagc
      721 acccccgacg aggacggagt cccccgcggt gccgcgaagc tccgcgcgca gaggctcgat
      781 cgccgcgtac ccctccttgc tgaacgggtc ggcttcgagc gtcacctcga aacgtgcgcc
      841 ggcctcgcca cgctccggct cggtcaccgc cgccacgccc ggcgccctgc gggcggctgc
      901 gagcgctgcg tcggtacggc tctcgtcccg cacgagcacg acggtcggcg cgctcgcgcc
      961 ggccgggaag gactgctcga gcagctcctg cccctgcacc gactcgaccg agccgcggaa
     1021 cgcattggct gtcgtgaggt cgtcgtcgag cgtcagggcg ccgagcgaca tgaccgcgag
     1081 tccaagtgcg gttgcgatcc agaccggacg gcgccgacgc tcgatccacc agcccaggcg
     1141 gcgccagatg cctgctcgct ctgccttggg ctcggttccg tagcggggaa cgaagggcca
     1201 aaaggcacga cgaccgccga ggagcagcag agccggcagg agggtgagca tggcggcggc
     1261 cgcgacggcg acgcccatcg cgcccaccgg gcccagaccc gcggtcgagt tgacacgcgc
     1321 aaacgagagg cagaggagag cggcaacgac ggtgcctgcc gacgcgagga ttgccggccc
     1381 cgcttgacgg accgcgatca gcatcgcctc gtgcttgtcc tcgacctgat ggagctcctc
     1441 gcggtagcgg gcggtcagaa gcaaggcgta gttggtaccg gcaccgaaca cgagcaccag
     1501 cagaatcccg cccgtctgcc cgttgatcac cacgcccgcc tcggcgagca gggagccgag
     1561 gccccgtacg accgcctccg cgaagaggac tgagacgagt ggcaggatcc agaagatcgg
     1621 gctgcggtag atgagaacga gcaggatgaa gacaaggccc gccgtcgcga acagcaacgt
     1681 cgagttgatc ccctcgaacg ccttgcttgc atcggccgag aagccggcgg gcccggtcac
     1741 cctggcctcg agctgcggcg gaaggccttg gcgcacgagc tcgcgggcgt cctcgacggc
     1801 accgatcaag acctcctcgt ccccgtcggc ctcgatcggg atggtcagca cggcggcgtc
     1861 gcccgcctgg gagtaggcgg gtgggggcag agactggacg ccgtcgatgc cggcggtcga
     1921 gattcgctgc cgcgccgcct cgacggcctc ctgtccttcg gcccccagtt cgccgggtgc
     1981 ccttatcacc gctatcgcgg gtgtcacgtc gcccgacggg aatccgtcgg cggccttcag
     2041 gacggcgacg gactccgcgt cgccggggag gaagctcgac ggctcgttgc gctgggcttg
     2101 ctcgaacttc cccgccagcg ggccgaggcc gacaaccagc acgagccaga gggcaagcac
     2161 gacgaacttg ctgcggcggc cggagatgac actcgcgatc ccaaacattg gacatactct
     2221 atacataact acctagaaac cagtttcaga caaagagata gatcacatcg ctgccgctgt
     2281 tggatccgcc ggcgcccgcc catcccgtac gcaacctgta cgaagctcct cggatctcct
     2341 accggacgca caagcgagag agccccgcat gcggggctct ctttttactt cgtcctgccg
     2401 cggtcggcgc ggcgttctcg tcgctactga atcagcgaca tcatcttgaa catcggcagg
     2461 tacatcgaga tgatgatgat gccgaccatc attccgacac cgaccatcat caacggctcg
     2521 atgatcgagg tcagcgcgga gatcgaggcg tcgacctcgt cctcgtagaa gtcggcgacc
     2581 ttggcgagca tctgctccag ctcacctgtc tcctcgccga tcttcaccat ctgaccgacc
     2641 atcggcggga aggcggcgct gtcgatcagc ggctgcgcga tcggcgcgcc ctcttggacg
     2701 cggatacgca tgtccatcgt ctcgcgctcg atgacccagt tgccggacgt ctgcgccgtg
     2761 atctcgagcg cccggatgat gtccactccc gaagcgacga gcgtggcgag cgtgcgggag
     2821 aagcgcgcca tcgcgatctt gcgcacgacc gtgccgatct gcatcggaat ccgcagcttg
     2881 aacacgtccc acacctgccg gccgcgctcg ctcttcttcc acttgacgaa gccgaagacg
     2941 ccggcaccga tcagcgggaa gatgatgaac cagtagccgc gaagcgcgtt ggagaccttc
     3001 aggacgtact gcgtgaggac gggaagctca ccgccgagct ggtcgaagat cttgacgaag
     3061 acgggaacga ggaacatcag catcccgacc atcacgagcg tcgcgaagac gaggacgacc
     3121 gacggataca tcatcgcgcc cttcacgcgg cgcctgatct tctgctcctt ctcgatctgc
     3181 gttgcgaccc gatcgagcac ggtgtcgagc acaccggcag cctcgccggc ctcgaccatg
     3241 gcgatgtaca ggcggctgaa gacctctggg tggccggcca tcgcctccga caggagagca
     3301 cccgactcga ctttgtcgcg gacgtcgtcg acgacgacct tcaacgcctt gtcgtcggtc
     3361 tgctgttcga ggatgacgag tgctgcgacc acgctcagcc cagcctcgat catcgtggcg
     3421 aactggcgcg aaaagatctg cagcgacttc gtcttcacct tcggctggaa gaatccacgc
     3481 ttgctgtccc cgccggcctc gcctccggag acctccgcga tgcttcgggt catcaacccc
     3541 cgaccacgga gcgcgtcacg cgcggccccg acgtcggcgg cctggatctc acccttcagc
     3601 tcgaagcctt gggcgttgat tgcgtcatag gcgaacgacg gcatctagag cacctccctc
     3661 ggcgtcgtcg gcttgctaat ccggctcact tgcccgctga gccctggtcg tcggcgggcg
     3721 ccgtgaactc ggccgtctgc tcgggcgtgg tacccgtgta cagcagcttg agcgtgtagc
     3781 gcacgccggt tgcctgatcg acgagcgtca gcgccttgcc catctgcagg ctcaccgcct
     3841 tgcggccgcc cgtgaagccg ccgtcgcccg cgatcgcgag cttcgcgtcg gtctcactga
     3901 gcttcgtgac cacgaagagg ttctgcggag tcgggaaggg cttcttgacc tcgagcgcga
     3961 cgggcgttcc gttcaggttg atcgtcgcga aggtaagaac gggaggcttg gcgggcttga
     4021 cgggatcggt cgggatggca ggggtcgtag ttccacccga accggagggt tcagtcgtgc
     4081 ccgtgccggg ctcgctcggc gttgtgccgg gcttgctcgg tgccggcggc accgaggagc
     4141 tggcattcgc agcctgcgtg ccgtcggagc cgtcgccgcc gcccgcgtcg accggcttgt
     4201 cgtcgacctg ctggacgaac gggtccttgg ccggcatccg gttgaagacc agcagacgac
     4261 cgtcgccgcc ctggtcggcg aagccgccgg ggaggacgac gccggccacg aaggccgtac
     4321 tggtcggctt ccc
//
LOCUS       DBNGNK010000085         4501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1590062,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000085 DBNGNK010000000
VERSION     DBNGNK010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4501
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1590062"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC150V_02215"
     CDS             complement(<1..204)
                     /locus_tag="AC150V_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761767.1"
                     /translation="MPRQARLTAAFLLATVLALAAVGHADALTCPDAALKERLDAADG
                     AFAGRIVSDRPGPGNTRVYRFIVD"
     gene            complement(210..1361)
                     /locus_tag="AC150V_02220"
                     /pseudo
     CDS             complement(210..1361)
                     /locus_tag="AC150V_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010197478.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
     gene            complement(1371..2390)
                     /locus_tag="AC150V_02225"
     CDS             complement(1371..2390)
                     /locus_tag="AC150V_02225"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR3761768.1"
                     /translation="MPVPPLLEELLRASAPSGAEEAASAIVRREAAALSASVEADALG
                     STVVRVAGTDGRRLLALFAHVDEIGMVVRSADANGLLQVARLGTWRAAHAAFQRVEVL
                     TARGPLPGVATHDGEGDVGWRGVRVDVGARDRVEALSLVAPGDPVVVAGVPVELYGDR
                     VAASSLDDRAGVYAALEALRGLTAEPPAWDVAFVATTQEEGSAHGAAAAAGVVRPDIS
                     VVIDVTYAGDAPGEPAWGDVSLGGGPVVFRGPPTHPLVARGLIEAAQAAGIAVAVEAG
                     PKTASDADAVATAAGGRAVGLLSIPLRNMHSAVEVVDLADVHATVKLVEAYARSLDPG
                     LDLTR"
     gene            2413..3882
                     /gene="glpK"
                     /locus_tag="AC150V_02230"
     CDS             2413..3882
                     /gene="glpK"
                     /locus_tag="AC150V_02230"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006359216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR3761769.1"
                     /translation="MPPTQQVVAAIDHGTSSTRCLLLRRDGTSVAEHRVEHPVGTPHP
                     GWVELDMAAVWSATRECLARALAAGRKTRADVAAVGIANQRESAVLWERATGRPVGPA
                     IVWQDTRTAAAVTELAEAGGIDRFRDRTGLPLSTYSTALKLRWLLDAAPHRQAAAERG
                     ELLFGTPDTWLVWNLTGGPAGGSHVTDPSNASRTLLMDIATLGWDDDLLDLMAIPRPV
                     LPEIRSSSEVYGTALGSLAGVPVAGILGDQQAALLGQGCVAPGQSKCTYGTGAFLLQT
                     VGPDPVRSESGLITTVAWQLGDEAATYALEGSVAVAGGVVQWLRDSLGLISEAAEIER
                     LARSVADSGGVVFVPAFSGLFAPYWRSDARGVIAGLTQHTGRGHIARAALEACAFQVA
                     ELVEAMRADTGGGRLEELRVDGGMTANELFLQLQADILGARVVAPAAREMTALGAGFA
                     AGLAVGFWPGADELPIDTTPRHVYEPTTGEDERRRALVR"
     gene            4065..>4501
                     /locus_tag="AC150V_02235"
     CDS             4065..>4501
                     /locus_tag="AC150V_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016593.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761770.1"
                     /translation="MTPLAVIPTDLLTPLAAYLRLRSSDRASFLLESVERGRLGRNSW
                     MGAGSRLVTFEQAETLELPVVGYLAYDHIAVLEPTVPLPEAGRGLPESRFVVADTLVR
                     FDHGAGIAEVLAGDPDDVAGRLARETPVPAGGAGTASGELMRFP"
BASE COUNT          665 a         1629 c         1590 g          617 t
ORIGIN      
        1 atcgacgatg aatcggtaga cgcgcgtgtt ccccggcccg ggccgatcgg agacgatccg
       61 gcccgcgaaa gcgccgtccg ccgcgtcgag ccgctctttc agcgccgcgt cggggcaggt
      121 gagcgcgtcg gcgtggccca cggccgcgag cgcgagcacg gtcgcgagga ggaaagcggc
      181 tgtgagccgc gcctgacggg gcacgagcct caggcctcga tcagcagcgc gtcgccctgg
      241 ccaccgccgg agcagatcgc agcgagcccg aggcccccgc cgttgcgccg gagctcgtgg
      301 acgatcgtcc cgagaatgcg gccacctgac gcgccgatcg gatgcccgag agcgacggcg
      361 ccgccgttga cgttcacgat ctcctcgtcg actccgagca tgcgggtcga gttcttcgca
      421 acggacgcga acgcctcgtt gatctcgacg cgcttcacgt ccgcgatgga ggatcccgct
      481 gcggcaagcg cccgctccgc cgcgcgggcc ggtgtccgcg cgaggtacgc gaaatcgtcg
      541 gcgacgtagc cctggccgag aatcgtcgcc agcggctcga ttccgcgcga ccgcgcatac
      601 gactccgagc agacgacgac gcatgacgcc ccgtcgttca cgcccggggc gttgcccgcc
      661 gtcgtcgtac cctcggagtc gaagacgggc tcgagcgaag caagcttctc gatcgtggtg
      721 tcgcggcgca ccgattcgtc ggcgacgacg tcgccgaccg gcaccagctc gtcctcgaag
      781 cgtcctgcat cctgagcctg ggcggcgcgc acgtgcgagc ggaaagccca ctcgtcctgc
      841 tcctcgcgcg tgatgccgag ctcacgcgag acgagcgaag cctgctgcac catgtgcagc
      901 ccgtcgaagc tcgacacgag cccgtcgaag accatgtgat cgaccacctc gccgttgccg
      961 agccgatacc cgaaccgtgc cttcggcagc aggtacgggg cattggacat cgactccatc
     1021 ccgccggtga cgacgacgtc gtgctgaccc gcgccgatca tgaggtcggc gatctcgacg
     1081 gcacggatcg aagacgcgca caccttgttg atcgtgtctg ccgggacgtc cgtcggaatt
     1141 ccggccgcga ccgccgcctg acgggccggc gcctggccca caccggcttg gaggacttgg
     1201 cccatgatcg cgtagccgac ctcgtccggc tcgattcacg cgcggtcgag ggccgcgcgg
     1261 atcgagatcg cgccgagctc ggtcgccggc accccggcaa gcccgccgcc gagccggccg
     1321 aagggcgtcc gcacggcggc gacgatgacg gacctgttca ctcggccagc ctagcgagtg
     1381 aggtcgagac ccggatcgag gcttcgcgcg taggcctcga ccagtttcac agtcgcgtgc
     1441 acgtcggcga ggtcgacgac ctcgacggcg ctgtgcatgt tgcgaagggg tatcgagaga
     1501 agcccgacgg cccgccctcc cgcggccgtg gccacggcgt cagcgtcgct ggcggtcttc
     1561 ggtcccgcct cgacggccac ggcgatcccc gcggcctggg cggcctcgat cagaccgcga
     1621 gcgacgaggg ggtgcgtggg cggcccccgg aagacaaccg gcccgccgcc aagggagacg
     1681 tcgccccagg cgggctcgcc tggcgcatcg cccgcatacg tcacgtctat cactaccgag
     1741 atgtcggggc ggacgactcc cgccgcggcg gccgctccgt gcgcgcttcc ttcctcctgc
     1801 gtcgtggcga cgaacgcaac gtcccaggcg ggcggctccg ccgtgagacc gcggagcgcc
     1861 tcgagggcgg cgtacacacc ggcgcgatcg tcgagcgacg aggcggccac ccggtcgccg
     1921 tagagctcca cgggaacccc ggccacgacc acggggtcgc ccggcgcgac caacgagagg
     1981 gcctcgaccc ggtcgcgggc gccgacgtcg acacgcacgc cacgccagcc gacgtcacct
     2041 tccccgtcat gggttgcgac accgggcagc gggccgcgtg cggtcaggac ctccacgcgc
     2101 tggaacgcgg catgcgcggc acgccaggtc cccagccgag cgacctggag caggccgttc
     2161 gcgtcagcac tgcgaacgac catcccgatc tcgtcgacat gcgcaaagag cgcgagcagg
     2221 cggcgaccgt ctgtcccggc gacgcggacg accgtcgacc cgagcgcgtc cgcctcgacg
     2281 gaggcgctca gtgcggcagc ctcgcgacgg acgatggccg aggcggcctc ctccgctccg
     2341 gacggggctg aagcacggag aagctcttcc agcaggggcg ggacaggcac ggcgcgagtc
     2401 tagactcgcg ccgtgccacc gacacagcag gtcgtcgccg caatcgacca cggcacatcg
     2461 agcacccgct gcctcctcct ccgccgggac ggcacatcgg tagcggagca ccgggtggag
     2521 cacccggtcg gcacgccgca tcccggctgg gtcgagctcg acatggcggc cgtctggtcg
     2581 gcgacccggg agtgccttgc cagagcactg gcagccggga ggaagactcg ggcggacgtc
     2641 gctgcggtcg ggatcgccaa ccagcgcgag tcggcggtgc tctgggagcg ggcgaccggt
     2701 cgacccgtcg gccccgcgat cgtctggcag gacacccgca cggcagcggc ggtcaccgag
     2761 ctggccgagg caggtgggat cgaccggttc cgggaccgga ccggcctgcc gctctcgacc
     2821 tactcgaccg cgctcaagct tcgttggctg ctggacgcgg cacctcatag gcaggcggcc
     2881 gccgagcggg gcgagctcct cttcgggacg ccggacacat ggctggtctg gaacctgacg
     2941 ggagggcctg ccggcggcag ccacgtgaca gacccatcga atgcgagccg taccctcctg
     3001 atggacatcg ccacgctcgg ctgggacgac gacttgctcg acctgatggc gattccgcgc
     3061 ccggtgctgc ccgagatccg gagctcgtcg gaggtgtacg gcaccgccct cggctcgctc
     3121 gccggagtcc ccgtggctgg aatcctgggc gatcagcagg ctgctctcct cgggcagggc
     3181 tgtgtggccc cgggccagag caagtgcacg tacggcacgg gcgccttcct gctgcagacc
     3241 gtgggcccgg atcccgtccg ctccgagtcc ggcctgatca ccaccgtcgc ctggcaactg
     3301 ggtgacgaag cggcgaccta cgcgctcgaa gggtcggttg ccgtcgccgg cggagtggtg
     3361 cagtggctgc gcgactcact cggcctgatc agcgaggccg ccgagatcga gcggctggcg
     3421 cgaagcgtcg cagacagcgg tggcgtcgtc ttcgtccccg cgttctcggg cctcttcgcc
     3481 ccctactggc gaagcgacgc gcgcggcgtg atcgcgggcc tcacgcagca caccggtcgc
     3541 ggccacatcg cacgcgccgc tctcgaggct tgtgccttcc aggtggcgga gctggtggag
     3601 gcgatgcgtg ctgacaccgg tggcggcagg ctcgaggaac tgcgggtcga cggcggcatg
     3661 accgcgaacg agctcttcct ccagctgcag gccgacatcc tcggcgcgcg cgtcgtcgcg
     3721 ccggcggcaa gagagatgac ggcgctcggc gcgggcttcg ccgccggcct cgcggtcggc
     3781 ttctggccgg gagccgacga gctgccgatc gacacaacac cacgacacgt ctacgagccc
     3841 acgaccggtg aggacgaacg tcgtcgggcg ctcgtccgct agcagaaggg cgtcgagcgg
     3901 acgctcgact ggcccggcga gatcgactac tctgaagcga gatgactccc gtccgcagct
     3961 gggcctggtc gtggcgcccc gagcgaggcg ggagcctgcg ctagcacgat ccgcgcgaca
     4021 gccctcctcg ctcgagacct ccggccggag gtctttctcg ttcgatgaca ccgcttgcag
     4081 tcatacccac cgatctgctg acacctctgg cggcgtacct ccggctgcgc tcctcggacc
     4141 gcgcgagctt cctgctcgag tccgtcgagc gtggccgctt gggccgcaac tcgtggatgg
     4201 gagcgggatc gcgcctcgtg acgttcgagc aggcggagac cctcgagctc cccgtcgtcg
     4261 ggtatctcgc ctacgaccac atcgccgtgc tcgagccgac ggtgccgctg ccggaggcgg
     4321 gccgcggact gccggagagc cggttcgtcg tggccgacac gctcgtgcgc ttcgaccacg
     4381 gtgcggggat cgccgaggtg cttgccggcg atcccgacga cgtcgccggt cgtctcgcgc
     4441 gcgagactcc cgtgcctgcc ggtggagcgg gcacagcgag cggcgagcta atgcgattcc
     4501 c
//
LOCUS       DBNGNK010000086         3904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1660681,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000086 DBNGNK010000000
VERSION     DBNGNK010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1660681"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..485)
                     /locus_tag="AC150V_02240"
     CDS             complement(57..485)
                     /locus_tag="AC150V_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761771.1"
                     /translation="MSSVPEKLLVVVLDEVDHDTLRNALGDSIRPGSTVAVLAPMSKL
                     SRIEWLTNEEDAERRSASDRAGATAEALDDATGSVEIAEVSVGASDPVLAVEDALRQY
                     VADRVVVFRANADETTWLEADLTERLGHLDAEVDEIVAST"
     gene            577..1557
                     /locus_tag="AC150V_02245"
     CDS             577..1557
                     /locus_tag="AC150V_02245"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3761772.1"
                     /translation="MLFADLRAAAAGVTQQDDPVSDVSPATEVADSASDLASTLLERV
                     PFVGIAALVVLVAWVISRGLRFVVHRWLSRSHPPSFATVMSKLTGWLFVALGVLVGLA
                     VTFPSVKPVDLLAGAGILSVAIGFAFKDILENLLAGVLILFRDPFRSGDQITVGDQTG
                     VVEQITIRETRIRTFDGRQVLIPNATVYTDVVVVETAHPYRRDAFKVAVAYGADLHVA
                     RRVSVAALRSTEGVKAQPAPEALVTKIEPACVEIEMRYWCTSRQAELVAVRDRAIQAV
                     KEALDANEVGMPADTIEVAATPSLLRALHNGTEGPRSEPGEPRRGPPVRS"
     gene            1604..2920
                     /locus_tag="AC150V_02250"
     CDS             1604..2920
                     /locus_tag="AC150V_02250"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR3761773.1"
                     /translation="MRWAGATTPTPSWHAVNKRWPAAFGLAATAWYARDTLRHRRESG
                     QGFDVGERTALDSETFRRVSEATTHAPLETARDIELLVNGVEIFPALLDTIARAKHSL
                     NLATYAYWSGQIAEEVAAALCERAEAGVKCNVLLDAFGSSQIDDALEDRLRRSGVQVV
                     YFRPLKPYALNRVNNRLHRRILVADGRVGSTGGAGIADEWTGNAQDSDHWRDSQVRLR
                     GPAVRMLQGAFAENWLEATGELLTGDSYFPHIDEPAEGAPIQLLRSSAGVGDTTLEVL
                     YYMVIASARRTLNVTTGYFAPRRAFVCALAEAAERGVDVRLLLPGPRIDKRMVRQAGR
                     SRYAALLGAGVRIFEYQPTMLHAKSVTADGVWSSLGSANFDNRSFQLNDEATLCVFDG
                     DFARRLDDAFVHDLSRAHEITLRAWRRRPLTDRFQENLTKVVQREL"
     gene            complement(2984..>3904)
                     /locus_tag="AC150V_02255"
     CDS             complement(2984..>3904)
                     /locus_tag="AC150V_02255"
                     /inference="COORDINATES: protein motif:HMM:NF037269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3761774.1"
                     /translation="GAAAALRREFRRYIERHASPGSDISGAELAVGEMVANVVEHGGG
                     RGWVQVEWPHDLKVTVYDLGTGFSLDEASLPAEALAESGRGLYLVSRFTSSLEVAARR
                     GGGTAVRTVIPIRRESSASLDPPRRRTQGLPAAEEADEDGAFPREAFLRALVVELAEH
                     LELEHGAAAAEAAVAQVGADVGARMEDAYRSARDIVGRLTPAQMGDLYVRLKAAIDGD
                     FYIIDATEERIVLGNRRCPFGEVVKRAPTLCRMTSSVFGGIAARNAGAAAVQLQERIA
                     VGDAECRVVVVLDPDAADVAGDFHSYSADD"
BASE COUNT          623 a         1346 c         1288 g          647 t
ORIGIN      
        1 caacttgcgc agcgcgtcac ctctggtcga ggtgaccagc gagcgaagta ggagcgctat
       61 gtcgacgcga cgatctcgtc cacttccgcg tccagatggc cgagacgttc ggtcagatcg
      121 gcctcgagcc acgtcgtctc gtcggcgttc gccctgaaga cgacgacccg atccgcgacg
      181 tactgacgta gcgcgtcctc gacggccagc accgggtcgc tggcgccgac cgagacctcg
      241 gcgatctcga ccgacccggt tgcgtcgtcc agcgcctcgg ctgtggcacc agcccggtca
      301 gaggccgacc tgcgctctgc gtcttcctcg ttcgtcagcc actcgatgcg ggacagcttg
      361 gacatcggag cgagcacagc gaccgtcgag cctgggcgaa tcgagtcgcc gagcgcgttt
      421 cgcagagtgt cgtggtcgac ctcgtccaac acgacgacga ggagtttttc cgggacgctg
      481 ctcatcacga cgagagcgta gtcaactgcg cccggggtcg cccggtgcca acgtgcaggc
      541 atgtttggca acgcctcgcg gcgggtaacg cgagtcatgc tgtttgccga cctgcgcgcc
      601 gccgctgccg gggtgacgca gcaggacgat ccggtctcgg atgtgagccc cgccaccgag
      661 gtggcggact ccgcgtccga cctcgcgagc accctgcttg agcgcgtgcc cttcgttggt
      721 atcgccgcgc tcgtcgtgct cgtcgcgtgg gtcatctcac gcggtctccg cttcgtcgtc
      781 caccgctggc tgtctcgctc gcatccaccg agcttcgcga cggtgatgag caagctgacc
      841 ggctggctct tcgtggctct cggcgtcctg gtcggcctcg ccgtcacgtt tccgtccgtc
      901 aagccggtcg acctactggc aggtgcggga atcctgtccg tcgcgatcgg cttcgccttc
      961 aaggacatcc tcgagaacct gctcgccggc gtgctcatcc tcttccgcga tcccttccgc
     1021 tcgggagatc agatcactgt cggcgatcag accggagtgg tggagcaaat cacgatcagg
     1081 gagacgcgca tcaggacgtt cgacgggcgt caggtgctga ttccgaacgc caccgtgtac
     1141 acggacgtgg tcgtcgtgga gacggctcac ccctatcgcc gcgacgcgtt caaggtcgcc
     1201 gtggcctacg gcgccgacct ccacgtcgcg cgccgcgtga gtgtcgccgc gttgaggagc
     1261 accgaagggg tcaaggccca gcccgcaccc gaagccctgg tgacgaagat cgagcctgcg
     1321 tgcgtcgaga tcgaaatgcg ctactggtgt acttctcgtc aagcagagct ggtggccgtc
     1381 cgcgacaggg cgattcaggc tgtcaaggaa gcgctcgacg cgaacgaggt cgggatgccg
     1441 gccgatacga tcgaggttgc cgccacacca agcctcctca gggcgttgca caacggcacc
     1501 gaggggccgc gctcggagcc cggcgagccg aggcgtggcc cgcccgttcg cagctagctg
     1561 cggccgcgcg tgtaggagga ttcagaacga tgtcttgtct accatgcgct gggccggcgc
     1621 tacgacccca acgccgtcgt ggcatgccgt gaacaagcgt tggcccgccg cgttcgggct
     1681 cgccgcaacc gcctggtacg cgcgcgacac cctccgccat cgacgagaga gcggtcaggg
     1741 cttcgacgta ggcgagcgga cggcgctcga cagcgagacg ttccgtcgcg tatcggaggc
     1801 gacaacgcat gcgccgctgg agactgcgcg cgacatcgag ctcctcgtca acggcgtcga
     1861 gatctttccc gcgctcctcg acacgatcgc gcgcgcaaag cactcactca acctcgcgac
     1921 ctacgcgtac tggagcgggc agatcgcgga ggaggtcgct gcggcgctct gcgagcgtgc
     1981 ggaggcgggt gtcaagtgca acgtcctcct ggacgctttc ggttcttcgc agatcgatga
     2041 cgcactagag gatcgcctgc ggcggtccgg ggttcaggtc gtctacttcc ggccgctcaa
     2101 gccgtacgca ctcaatcgag tcaacaatcg cctccaccgc cgaattctgg tggcggacgg
     2161 ccgagtcggc tcgaccggag gtgccggcat cgcagacgag tggacgggga acgcacagga
     2221 ctcagaccac tggcgggaca gccaagtgcg cctacgcggg ccggcggttc ggatgctcca
     2281 gggggcattt gccgagaact ggctcgaagc gaccggtgag cttctgacgg gagactcgta
     2341 cttcccgcac atcgacgagc ctgctgaagg agctccgatt cagcttctgc gttcgagcgc
     2401 aggcgttggt gacacgacgc tggaggtcct gtactacatg gttatcgcgt cggctcggcg
     2461 aacgctcaac gttacgaccg ggtacttcgc gcctcgcagg gcgttcgtct gcgcccttgc
     2521 agaggccgcc gagcgcggag tggacgttcg gctcctcctg cccggcccgc gcatcgacaa
     2581 gaggatggtt cgccaggcgg gacgaagccg gtacgccgcg ctgctcggag ccggcgttcg
     2641 tatcttcgag taccaaccaa ccatgctcca cgcgaagtcc gtgaccgccg acggtgtctg
     2701 gtcgtcgctg ggctcggcga acttcgacaa tcggtcgttt cagctcaacg acgaggcgac
     2761 actctgcgtc ttcgacggtg atttcgcccg tcgtctcgac gacgcgttcg tgcacgatct
     2821 gagcagagcg cacgagatca cccttcgcgc gtggcgccgc cggccgctca ccgatcgttt
     2881 ccaggagaac ctgaccaagg tcgtccaacg cgagctctag cgagaagctc gccggcgacg
     2941 ggcgtcgggc agccctcgca atcggccgga accgcccctt gccttagtcg tcggccgaat
     3001 agctgtggaa gtccccggca acgtcggccg cgtcgggatc gagaacgacg acgactcggc
     3061 actcggcatc tccgaccgca atgcgctcct ggagctgaac cgcggcggcg ccggcgttcc
     3121 gggctgcgat ccctccgaac acgctcgagg tcatgcgaca gagcgtcgga gcgcgcttca
     3181 cgacctcgcc gaagggacag cgccggttgc cgagcacgat gcgttcctcg gtggcgtcga
     3241 tgatgtagaa gtcgccgtcg atcgcagcct tgagacggac gtagaggtcg cccatctgag
     3301 ccggcgtcag gcggccgacg atgtcgcgcg cgctccggta cgcgtcttcc atccgtgcgc
     3361 ctacgtcggc acccacctgc gccacggccg cctcggcggc cgccgcgccg tgctcgagct
     3421 ccagatgctc agcgagctcg acgacgagcg cccgcaagaa ggcctcacgc gggaacgccc
     3481 cgtcctcgtc ggcctcctcg gccgccggga gtccctgcgt gcgtcgccgc ggaggatcga
     3541 ggctcgccga cgattcccgc cggatcggaa tcacggttct caccgctgtc ccgccaccgc
     3601 gccgtgccgc gacctcgagc gagctcgtga accgcgagac gaggtacagg ccacgcccgc
     3661 tctccgcgag cgcctcggcg ggaagcgacg cctcgtccaa cgaaaagccg gtaccgaggt
     3721 cgtacacggt taccttcagg tcgtggggcc actcgacctg cacccagccc cgtcccccgc
     3781 cgtgctcgac gacgttggcg accatctcgc cgactgcgag ctccgcgccg gagatgtcgg
     3841 agccgggtga cgcgtgtcgc tcgatgtagc gacggaactc acgccgcagt gcggcggccg
     3901 ctcc
//
LOCUS       DBNGNK010000087        13003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_730205,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000087 DBNGNK010000000
VERSION     DBNGNK010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_730205"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..275
                     /locus_tag="AC150V_02260"
     CDS             153..275
                     /locus_tag="AC150V_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761775.1"
                     /translation="MKYVLLAEAAAAAADELEQKVRSGRRRRADSDLRTRTVAV"
     gene            334..1332
                     /locus_tag="AC150V_02265"
     CDS             334..1332
                     /locus_tag="AC150V_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019907222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3761776.1"
                     /translation="MKTSLGIWALSSMVTRFVPGGYQPQHAGESTAERVQRAVQGLGD
                     LMDDYEFHYPQELSEENLDEVRAALDGHGIYAVATGTHLNPSFGKGALSSPDDKVRQA
                     ALEEALRAAEFAGSVGAQMIIWPGIEGYNYPFQTPYQDSWARFVDGVGQVAARCGENG
                     VTLFLEHKNSEPAMKIFMRNIGMTLHVIHKLQAQGIGNVQVNMDWQHLLMNGESLAEY
                     AALLAAEGLLGHQHANSGWGTFDDDNMVGATAFMETLELALELRRADYGANGERLGFD
                     LYPYTEDAVQAVARSVHQWNFIWDVSGRIDDAALGEAQAQKDAVRAYELVYAALGA"
     gene            1343..2779
                     /gene="xylB"
                     /locus_tag="AC150V_02270"
     CDS             1343..2779
                     /gene="xylB"
                     /locus_tag="AC150V_02270"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR3761777.1"
                     /translation="MTVSVGLDVGTTGVKAIAVSPTGDVIARAERGYPLSTPQPGWSE
                     QDPEDWWRATDECLAEVSREREVAGIGLSGQMHGLVALDSRDRVLRPAILWNDQRTAA
                     ECEEIEDALGLERLIELTGNRALTGFTAPKLLWLRRHEPDVYAAVSRVCLPKDYVRLR
                     LTGEWAIDVADASGTLLLDVRNRRWSDGVLDALALPAGILPPVHESPVVTGRTAGGIP
                     VAAGAGDCAAAALGVGIDRPGALSIVLGTSGVVFAALPSFAADAEARVHTFCHAVPGA
                     WHAMGVMLSAAGSLQWFRDRLAPDVPFDTLALEAAAWEPGADGLVFLPYLAGERTPHA
                     DPDARGAFTGLALHHDRAALVRAVLEGVAFGLRDSLDLLTSLGVEAELARVSGGGARS
                     VVWLEIVASVLGIPLETTAAEEGSAYGAALLGGVCGGVFADVHEAVSACVRPGRRFEP
                     RAAWAPAYADLLGRYRALYPALEHTEDL"
     gene            2776..3489
                     /locus_tag="AC150V_02275"
     CDS             2776..3489
                     /locus_tag="AC150V_02275"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3761778.1"
                     /translation="MSSLDGKVAVITGASRGIGAAVARALDDRGVKLGLASRSGDDLG
                     MADAVALPTDVRDRQKVTALVDATAERFGGLDILVANAGVGAYGPLVELDPGHLEEMI
                     DTNVKGVLYAATAAVPHLAARGGGDIVSVASEAGRRGLPNEAVYCASKFAQVGFTRAL
                     DHELRPQGIRCSNVCPGGVATDFALGEGRGRTPDMPELAGMMSAEDVADAVVFVLTRP
                     PNHRVLEVAFRPVSEESWG"
     gene            3495..4460
                     /locus_tag="AC150V_02280"
     CDS             3495..4460
                     /locus_tag="AC150V_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3761779.1"
                     /translation="MRLGLLSTAAINDEIIAGAAASDRVDIVAVGSREQRRAESYAAE
                     KGIARAFGSYDDLLADEATEAVYISLPNGMHHEWTMRALAAGKHVLCEKPYTRNPAEV
                     DEAFAAADDAGLVLMEAFMYRHHPQTRQLASLVEEGAIGRLVAVVATFSFVLDDLTDI
                     RALPELDGGALMDLGCYCVSNSRLLAGEPLSVGAVQVTGPTGIDMSLFGTMRFDDDVV
                     AQFSTSFAAPRRQYLEAIGEEAVICVHAPWRVDWPGELQLLRDGETETLDVPAVDSYA
                     LQLENMADAIAGSAPALLGKDDALGQARAIEALYRAAETGTVVKL"
     gene            complement(4468..4851)
                     /locus_tag="AC150V_02285"
     CDS             complement(4468..4851)
                     /locus_tag="AC150V_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR3761780.1"
                     /translation="MVIPASVREALAKHASDEAPNEACGLIVLSDGAAVRYEPGINAQ
                     PSPYRFELEMPDPEVWFLADDGYELAIFHSHLSSPPRPSRTDVENIGHWKGLPYVIYT
                     LRTDELAAWRITDDGIEPLALQPER"
     gene            complement(4845..6059)
                     /locus_tag="AC150V_02290"
     CDS             complement(4845..6059)
                     /locus_tag="AC150V_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3761781.1"
                     /translation="MTAVRIPPTLRNDVGGARQVDATGSTVRELLDDLAERYPSLGAQ
                     ILENGDIAPFVNVYLGGEDVRTLDGLDTAVPEGQTLILLPAMAGGELRAPGLVAESLL
                     ELVGNTPLVELPRLSPKPTVKIYAKLEGQNPSGSIKDRIAKSMIESAEASGELEPGRA
                     LLEPTSGNTGIALALVAKLKGYPLTVVMPENATEERMRLLRLYGADIVLSSGDEGSNG
                     AVRHALELAERDPKYFMPFQYGNAGNVRAHYEGTGAEIADALERVDVLVAGLGTGGTL
                     MGAGERLRESFPDVTVAAAEPLPGDPVMGLRSLGDGYVPPILDVSKLDRKVLVSNADS
                     VAALKRLLGEEGVFGGVSSGAVLHVGRRLAGELDEGVVVCVLADAGWKYLSADFWDTT
                     DVEESMDKTVWW"
     gene            complement(6056..7234)
                     /gene="moeB"
                     /locus_tag="AC150V_02295"
     CDS             complement(6056..7234)
                     /gene="moeB"
                     /locus_tag="AC150V_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR3761782.1"
                     /translation="MTTYRELLAQIKTEIAEVGTPETHSRLQNGGDPFLLDVREPDEW
                     NEGHLPGAVHVPRGNLESRIEALLPDRDREIIVYCASGNRSAFAAKTLAELGYEHVVN
                     LAGGFVDWKRNGFEVVTPKVLEPAQRTRYARHLMIPEVGEEGQQKLLESRILLIGAGG
                     LGSPASLYLAAAGVGTLGIVDADVVDDSNLQRQIVHSTERLGEAKVQSAKRTIEALNP
                     DVHVAAYEERLTSDNVERILSDEWDVIVDGADNFPTRYLVNDASIWHDVPVVHGSIYR
                     FEGQVTVFTPHQGPCYRCLYPAPPPPELAPSCAEGGVLGVLPGIIGSLQASEALKIVL
                     GVGDTLEGRLLLFDALHTTFDEVTVRRDPGCPVCGDSPTITDYVDYVQFCAAPAGVAT
                     "
     gene            7320..8303
                     /locus_tag="AC150V_02300"
     CDS             7320..8303
                     /locus_tag="AC150V_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3761783.1"
                     /translation="MSARARVVGVVALFAALGAAAVVGIVVAQTSGDERSTQAVAEPE
                     ERPQGAPPLALELGFRDDAEAAELRRATELYEQGRRAQARAIFDRYDSLEAKVGAAFA
                     AWPDDTLKLAGRLAALNPRSALVQLHLGLARFWAGEPGAEEAWLEAVSVEPDTPAAVT
                     ASDLLHPNFAPGLPVFVPEFDPPAAITSLPPAKQLPALAQAARAGGTEERLLYGIGLQ
                     RVGRPVSAAREFAAAAEAAPKRVDAQVAAAVGRFDKARPEEAFSRLGPLSRRYPDSAT
                     VRFHLGLLLLWSGDVEEAVRQLELANQIEPGSPLAGEATRYLERLASNGKG"
     gene            8338..9465
                     /locus_tag="AC150V_02305"
     CDS             8338..9465
                     /locus_tag="AC150V_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3761784.1"
                     /translation="MPSLRLPSCRTRWKGDFLSAPAASSTRTVVEDVLNTDEARALLE
                     AGRASGRLEAAEIALALDELDLDAAQMDEFYSALDEAQIEVVDGQAAEAPAAEPETEI
                     STDTLQLFLKDIGKVPLLTAAQEVELAKRIERGEHSAKQAMVEANLRLVVSIAKRYRN
                     QGLPFLDLIQEGTIGLVRAAEKFDHRKGFKFSTYATWWIRQAVARAIADKGRTIRMPV
                     HVVEKLNKIHRSERKLRAELSREPTIPEIAADLELAIEEVEQIRRSAQTPVSLEKPVG
                     DEEESEFGHFLTDETAPLPDEAAEAALRREALRSILGSLSQRERQVLELRYGLDGQQP
                     RTLDEVGKTFNVTRERIRQIEHQSLKKLRALAEASAVRDVA"
     gene            9514..10851
                     /locus_tag="AC150V_02310"
     CDS             9514..10851
                     /locus_tag="AC150V_02310"
                     /inference="COORDINATES: protein motif:HMM:NF019307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3761785.1"
                     /translation="MPSLDMLVDELAELIAIPSVSADEQHAADVRRAAKWVCDHIRAA
                     GGEADVVDQEGKPLVVGELRASRNAGTAPTVLCYGHFDVQPPDPLDLWDSEPFELTQR
                     DGWLYARGVADDKGQLFMLLKAAGLLAAAGELPVNVRFACDGEEETGGHAIVDWAAAD
                     ERGADVAIVFDSSMTSPGVLEFNIAMRGMCYLHLTLRTGERDLHSGLYGGAALNALHA
                     LVRALSSVLADPLTGRVPEPLRAGIVPPGSAEVRGWESLQPGADMLATQGALPMDERA
                     AEEFYVRTWAEPTVEVNGIEGGSPRLQKTVLPVEARANVSLRLAPGQSPDEIVPVFEQ
                     LIRDALPAGAELELERWATGEPTLVAPDQPAIELARAAVEETVGVAPLLVRSGGTIPV
                     VATLAARGIPVVVTGFAMVDSHLHSPNERLTAEYLPLGIETAQALFRRFEQRA"
     gene            10844..12115
                     /gene="pepT"
                     /locus_tag="AC150V_02315"
     CDS             10844..12115
                     /gene="pepT"
                     /locus_tag="AC150V_02315"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HIR3761786.1"
                     /translation="MPSAEYSSPLAAELAEDVLDRFLRYVRISTPAVPDSDTYPSNPG
                     QLDLSRLLVAELDEAGLEDVELTEHGYVFATLPGTAANAPTVGLMAHVDTAPDAPGDD
                     VRPIVHHAWQGEKITLPGDPAQILDPDDNPALAERVGHDIVTSDGTTLLGADDKAGVA
                     AIMAGVAHLAASDEARATVRVAFTVDEEIGLGTEHLDLDHFGADVAYTLDGSGLGELE
                     VETFSAKQVTVTFRGVAVHPGIGKARLVNAIKLASAFVSSLPQGELSPETTEGREGFV
                     HPVRVTGDARKLTVSLLARDHDEQLLEEHVILLRRLAEVAIADEPRASVDVEAVTMYK
                     NMAPVLERHPEVVGAAEEAIRRAGVEPVHTIIRGGTDGARLSERGLPTPNLFTGGQDY
                     HSLREWASVQDMAAAAATVVELVRVWGERTT"
     gene            complement(12134..12496)
                     /locus_tag="AC150V_02320"
     CDS             complement(12134..12496)
                     /locus_tag="AC150V_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3761787.1"
                     /translation="MDTLSTRTMLPVHEAETCSVAACAAIVGAKWTVLIVHDLSEGPR
                     RFTELEHSCLGISPRTLAERLRWLEAEEIVLRQSYAESPPRVEYELTQKGRALLPIIE
                     AMRSYGHEWLGCGVHEHD"
     gene            complement(12539..>13003)
                     /locus_tag="AC150V_02325"
     CDS             complement(12539..>13003)
                     /locus_tag="AC150V_02325"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3761788.1"
                     /translation="FAELGIACSVQFSHAPADRDLADRYWPDVSAGAYAYRSLWDAGA
                     VVANGSDAPVEELDPLAGIGAAVLRTNDDRPAWHPEQALTVEQALQASTVAPAWLAGD
                     ERRRGRLLPGFLADLVVLSGDPIDCPPDELGGIEVMATMVGGRWVYGAPPWD"
BASE COUNT         1990 a         4455 c         4538 g         2020 t
ORIGIN      
        1 ggaggtgatc gacgtgaagg acgcattcag aggcaaccgc ctcgtcttcg ccctgaccct
       61 gctcgtcatc tggttcgtga ttgccttgga ggtcatcttc ctcctcgccg gtgatcagcc
      121 gcccgtcccg cgctaggggc gcggcagcca gcgtgaaata cgtcctcctc gccgaagcgg
      181 cggcggcggc ggcggacgag ctcgagcaga aggttcgatc tgggcgtcgg cgacgagcag
      241 actccgatct tcgcacgcgg acggtggccg tttgacagcc gctccgccgg tggactacgt
      301 tggccgctcg cgagcgtacg ctcgcgcccc tcgatgaaga cctcactcgg catctgggcg
      361 ctcagctcga tggtcacccg cttcgtgccg ggcggctacc agccccagca tgccggcgag
      421 tccacggcgg aacgggtgca gcgcgccgtc caaggtctcg gcgacctgat ggacgactac
      481 gagttccact acccgcagga gctgtcggag gagaacctcg acgaggtgcg agccgcgctc
      541 gacgggcacg gcatctacgc ggtcgcgacc ggtacgcacc tgaacccgtc gttcggcaaa
      601 ggcgcactct catcgccgga cgacaaggtt cgacaggcgg cgctggagga ggcgttgcgg
      661 gccgccgagt tcgcaggctc ggtcggggcg cagatgatca tctggcccgg aatcgagggg
      721 tacaactacc cgttccagac gccctatcag gactcgtggg cgcgcttcgt cgacggtgtc
      781 ggtcaggtcg ccgcccgctg cggtgagaac ggcgtcacgc tcttcctcga gcacaagaac
      841 tccgagccgg cgatgaagat cttcatgcgc aacatcggca tgacactgca cgtgatccac
      901 aagctgcagg cacaggggat cggcaacgtt caggtcaaca tggactggca gcacctgctg
      961 atgaacggcg aaagcctcgc cgagtacgcg gcgctactgg ctgccgaggg cctcctgggg
     1021 caccagcacg ccaactcggg ctggggaacc ttcgacgacg acaacatggt aggcgcgacg
     1081 gcgttcatgg agacgcttga gctggcgctc gagctgcgcc gggcggacta cggcgcgaac
     1141 ggtgagcgac tcggcttcga tctctacccg tacacggagg acgcggtgca ggccgttgcc
     1201 cgctccgtgc accagtggaa cttcatctgg gacgtctcgg gccgcatcga cgacgcggca
     1261 ctgggggaag cgcaggcgca gaaagacgcg gtgcgcgcct acgagctcgt atacgcggct
     1321 ctcggcgcgt agcccacgga cgatgaccgt ctccgtcggt ctggacgtcg gcaccaccgg
     1381 cgtgaaggcg atcgccgtct cgccgaccgg tgacgtcatc gcccgtgccg agcgtggcta
     1441 tccactctca acgccacagc cgggctggtc ggaacaggac cctgaggact ggtggcgggc
     1501 aactgacgaa tgcctcgcag aggtgtcgcg ggagcgcgaa gttgcgggaa tcgggctgtc
     1561 gggacagatg cacgggcttg ttgcgctcga ctcgcgggat cgggtgctgc ggccggcgat
     1621 tctctggaac gaccagcgga cggccgccga gtgcgaggag atcgaggacg cgctcggtct
     1681 ggagcggctg atcgagctca ccggcaatcg ggcgctcacg ggcttcaccg cacccaagct
     1741 cctctggctg cggcggcacg agcccgacgt gtacgcggcc gtctcccgag tctgtctgcc
     1801 gaaagactac gtccgcctgc gcctcacggg agagtgggcg atcgacgtgg ccgacgcctc
     1861 cgggacgctt ctcctcgacg ttcgaaaccg acggtggtcg gacggagtgc tggacgcgct
     1921 ggcgctgccg gccggcatcc tgcccccggt gcacgagtct ccggtggtca ccgggcgcac
     1981 ggcgggcggg attccggtcg ctgcaggagc gggggactgc gccgctgcag cactcggggt
     2041 cgggatcgac cggcccggag ccctctccat cgtgctcggc acgtccggtg tcgtctttgc
     2101 ggcgctgccg agcttcgccg ccgatgccga agcccgcgtg cacacgttct gccatgccgt
     2161 cccgggcgcc tggcatgcga tgggagtcat gctctcggcc gcggggtcct tgcagtggtt
     2221 ccgcgaccgg ctcgcgccgg acgtcccctt cgacaccctc gccctggagg ccgccgcctg
     2281 ggagcccggc gccgacgggc tcgtcttcct gccctatctc gcaggtgagc gcacccctca
     2341 tgcggatccc gacgcccgtg gcgcgttcac cgggctcgcg ctccatcatg accgggccgc
     2401 cctggtgcgc gcggtgctcg agggtgtcgc tttcggcctg cgcgactcgc tcgacctgct
     2461 cacgagcctc ggcgtggagg cggagctcgc acgcgtttcg ggcggtggtg cacgttcggt
     2521 ggtctggctc gagatcgtcg cgtcggttct cggcatcccg ctcgagacga ccgcggcgga
     2581 ggaaggttcg gcctacggtg cggctctgct cggaggcgtc tgtggcggcg tcttcgccga
     2641 cgttcacgag gccgtgtcgg cctgcgtgcg gcccggccgg cgcttcgagc cccgcgctgc
     2701 ctgggcgccc gcctacgctg atctccttgg ccgctatcgc gccttgtacc cggcgctcga
     2761 acacacggag gacctatgag cagtctcgac gggaaggttg cagtgatcac cggcgcgagt
     2821 cgcggcatcg gtgctgcggt tgcacgcgcg ctcgacgaca ggggagtgaa gctcgggctc
     2881 gcctcgcggt cgggagacga cctcggcatg gcggacgcgg tcgcgctgcc gacagacgtg
     2941 agggatcgcc agaaggtgac ggcgctggtc gacgccaccg cggagcgctt cggcgggctc
     3001 gacatcctgg tcgcaaatgc cggcgtcggc gcctacgggc cgctcgtcga gcttgatccc
     3061 ggccacttgg aggagatgat cgacacgaac gtcaaaggtg tgctgtacgc ggcgaccgca
     3121 gccgtaccgc acctcgccgc ccggggcggc ggcgacatcg tctcggtcgc gtccgaggcg
     3181 ggccggcggg gcctgccaaa cgaggccgtg tactgcgctt cgaagttcgc tcaggtcggc
     3241 ttcactcgcg cgctcgacca cgagctgcgg ccgcagggga tccggtgttc caacgtctgc
     3301 cccggcggcg tggctacgga tttcgcgctc ggcgagggcc gcggcaggac gcccgacatg
     3361 ccggagctgg cggggatgat gagcgccgag gacgtcgcgg atgccgtggt cttcgtgctc
     3421 acgcgcccgc cgaaccaccg cgttctcgaa gttgcgttcc ggcccgtaag cgaagagtcc
     3481 tgggggtagc agacatgcga ctaggccttc tctccaccgc tgctatcaac gacgagatca
     3541 tcgccggcgc ggcggcctcc gatcgtgtcg acatcgtcgc tgtagggagc cgcgagcagc
     3601 gtcgggcaga gtcgtacgcc gccgagaagg ggatcgcgcg cgccttcggc tcgtacgacg
     3661 acctgctcgc cgacgaggcg accgaggccg tctacatctc ccttcccaac gggatgcatc
     3721 acgaatggac gatgcgagcg ctcgccgccg gcaagcacgt gctctgcgag aagccctaca
     3781 cgcgtaatcc cgccgaggtc gacgaggctt tcgcggcggc cgacgacgcc gggctcgtgc
     3841 tgatggaggc cttcatgtac cgccaccatc cacagacccg gcagcttgcc agcctggtcg
     3901 aggagggagc gatcggaaga ctcgtggcgg tggtggcgac gttcagcttc gtcctggacg
     3961 acctcacgga catccgagca ctgccggagc tcgacggcgg cgcgctgatg gacctggggt
     4021 gctattgcgt cagcaactcc cggctgctgg ccggcgagcc gttgtccgtc ggggcggtgc
     4081 aggtcacggg cccgacaggg atcgacatga gcctgttcgg gacgatgcgg ttcgacgacg
     4141 acgtggtcgc acagttcagc acgtccttcg cggcgcctcg gcgccagtac ctcgaggcca
     4201 tcggcgagga ggcggtcatt tgcgtccacg ccccctggcg cgtcgactgg ccgggggagc
     4261 tccagcttct acgcgacggc gagacagaga cgctcgacgt accggcggtc gactcctatg
     4321 cgctgcagct cgagaacatg gccgacgcga tcgctggatc ggcaccggcg ctgctgggga
     4381 aggacgacgc cctcgggcag gcgcgtgcga tcgaggcgct ctaccgcgcg gccgagaccg
     4441 gcaccgtcgt gaagctgtag gtcgccgtca gcgttccggt tgcagcgcaa gcggctcgat
     4501 cccgtcgtct gtgatgcgcc aggcggcgag ctcgtccgtg cgcagcgtgt agatgacata
     4561 ggggagcccc ttccagtgac cgatgttctc gacatccgtg cgcgacggcc gcggggggga
     4621 cgagaggtgg gagtggaaga tcgccagctc atagccgtcg tcggcgagaa accagacctc
     4681 cggatcgggc atctccagct cgaagcggta cggggatggc tgcgcgttga tgccgggctc
     4741 gtatctgaca gcggccccgt cggaaaggac gatcaagccg caggcctcgt tgggcgcctc
     4801 gtcgctggcg tgtttcgcga gcgcctcccg caccgaggcg gggattacca ccagacggtc
     4861 ttgtccatcg actcctcgac gtcggtcgtg tcccagaagt cggcggagag gtacttccag
     4921 ccggcgtcgg cgagcacgca gacgacgaca ccttcgtcca gctcgcccgc caggcggcgg
     4981 cccacatgga gcaccgcgcc ggacgagacg ccgccgaaga ccccttcctc gcccaagagg
     5041 cgcttcaacg cggcaacgga gtccgcgttc gacacgagca ctttgcggtc gagcttggac
     5101 acgtcgagga tcggtggcac gtagccgtcg ccgagcgagc ggagccccat caccggatcg
     5161 cccggcagcg gctcggcggc ggcgacggtg acgtccggga acgactcgcg caggcgctcc
     5221 ccggcgccca tgagcgttcc gccggtgcca agcccggcca cgaggacgtc gacccgctcg
     5281 agcgcatcgg cgatctcggc cccggtgccc tcgtagtgcg cgcgcacgtt gccggcattg
     5341 ccgtactgga agggcatgaa gtacttcggg tcgcgctccg caagctccag cgcgtgccgg
     5401 acggcgccgt tcgatccctc gtcgccggac gagaggacga tgtccgcgcc gtagagtcga
     5461 agcagccgca tccgctcctc ggtggcgttc tcgggcatca cgaccgtgag cgggtacccc
     5521 ttcagcttcg ccaccagcgc gagagcgata ccggtgtttc cgctcgtggg ctcgagcaga
     5581 gcgcggcccg gctcgagctc gccggaggcc tcagcggact cgatcatcga cttcgcaatg
     5641 cgatccttga tcgatcccga cggattctgg ccctcgagct ttgcgtagat cttgaccgtc
     5701 ggcttcggcg acaggcgggg cagctcgacg aggggagtgt tgccgacgag ctcgagcagc
     5761 gattcggcca cgagccccgg cgcgcgaagc tcgccgcccg ccatcgccgg caggaggatc
     5821 agcgtctggc cctccggcac cgcggtgtcg aggccgtcga gcgtgcgcac gtcctctcct
     5881 cccagataga cgttgacgaa gggggcgatg tccccgttct cgaggatctg cgcgccgagc
     5941 gacggatagc gctcggcgag gtcgtcgaga agctcacgga cggtcgaccc tgttgcgtcg
     6001 acctgtcgtg cgcccccgac gtcgttccgc agggtcggtg ggattcggac tgccgtcacg
     6061 tagcgactcc cgcgggcgcg gcgcagaact ggacgtagtc gacgtagtca gtgatcgtgg
     6121 ggctgtctcc acagacgggg cagcccgggt cgcgccgcac ggtcacctcg tcgaaggtgg
     6181 tatggagcgc gtcgaacagg agcagccgcc cctcgagcgt gtcgccgacg ccgagcacga
     6241 tcttcaacgc ctcgcttgcc tggagcgagc cgatgattcc gggaagcacg ccgagaacgc
     6301 ctccctcggc gcagctcgga gcaagctcgg gcggaggtgg cgcggggtag agacagcggt
     6361 agcagggacc ctgatgcggc gtgaataccg tcacctggcc ctcgaatcga tagatcgagc
     6421 cgtggacgac cgggacgtcg tgccagatcg aggcgtcgtt cacgaggtag cgggtgggga
     6481 agttgtccgc tccgtcgacg atcacgtccc attcgtcgct gaggatccgc tcgacgttgt
     6541 cggaggtgag ccgctcctcg tacgcggcta cgtgcacatc cgggttgagc gcctcgatcg
     6601 tgcgcttcgc cgactgcacc ttcgcctctc cgagccgttc cgtcgagtgg acgatctggc
     6661 gctggagatt cgagtcgtcg acgacgtccg cgtcgacgat gccgagcgtg ccgacgccgg
     6721 cggcggcgag atagagagag gcgggcgagc cgaggccgcc cgcgccgatc aggagaatcc
     6781 gtgactcgag gagcttctgc tggccttcct cgccgacttc cggaatcatg aggtggcgcg
     6841 cgtaccgtgt ccgctgcgcc ggctcgagca ccttgggcgt gacgacctcg aagccgttgc
     6901 gcttccagtc gacgaagccg cccgcgaggt tgacgacgtg ctcgtagccc agttcggcga
     6961 gcgtcttcgc cgcgaaggcg gaacggttgc cgctcgcgca gtagacgatg atctcgcggt
     7021 cgcgatcggg gagcagagcc tcgatgcgcg actcgagatt tccgcgcggc acgtggactg
     7081 cgcccggcag atgtccctcg ttccactcgt ccggctcacg gacgtcgagg aggaacgggt
     7141 cgccgccgtt ctgcaggcgg gagtgggtct cgggcgtgcc gacctcagcg atctcagtct
     7201 tgatctgggc gagcagctct cggtaagtcg tcattacttt cgcaagtata gcgtcggctg
     7261 gcggcgcacc ccccttgcaa cgcagcgacg cgccgattgt tcccggctac cctcaattcg
     7321 tgagcgcccg tgcccgtgtg gtcggcgtcg tcgccctgtt cgccgcgctc ggcgcagccg
     7381 ccgtcgtcgg aatcgtcgtc gcccagacat cgggagacga gcgctcgacg caggccgtcg
     7441 ccgagcccga ggagagaccg cagggcgcgc cgccgttggc actcgagctc ggcttccgcg
     7501 acgacgcaga agcagccgag ctccgccgcg cgacggagct ctacgagcag ggacgacgtg
     7561 cgcaggcgag agcgatcttc gaccgctacg actcgctcga ggccaaagtc ggcgcggcgt
     7621 tcgccgcctg gcccgacgac accctcaagc tcgccgggcg cctggcggcg ctgaacccgc
     7681 gcagcgccct cgttcagctg cacctcgggc tggcccgttt ctgggcgggg gagccgggcg
     7741 ccgaggaggc ctggctcgaa gccgtgtccg tcgagccgga cacgcccgcg gccgtgaccg
     7801 cgagcgatct gttgcacccg aacttcgcac ccggcctgcc ggtcttcgtt cccgagttcg
     7861 acccgcctgc cgcgatcacg tcgctccctc cggcgaagca gctgccggct ctcgcccagg
     7921 ccgcccgagc gggcggcacc gaggagcggc tgctctacgg gatcgggctg cagcgcgtcg
     7981 gccggcccgt ctcggctgcc cgcgagttcg cagcagccgc cgaggcagcc ccgaaacgcg
     8041 tcgacgcgca ggtagccgcc gcggtcgggc gtttcgacaa ggctcgaccc gaagaggcct
     8101 tctcgcgcct cggtccgctc agccgccgct accctgactc agctacggtg cgatttcacc
     8161 tcggcctgct tctcctctgg agcggcgacg tcgaggaggc cgtacgtcag ctcgagctgg
     8221 ccaaccagat cgagccgggg tcaccgctcg ccggtgaggc cacccgctat cttgaacggc
     8281 tcgcgtcgaa cggaaagggg taggcattca gcatcaccgg ggaggttttt ttgacgcttg
     8341 ccgagtctgc ggctaccgtc atgccgtacg cgatggaaag gggacttcct gagcgcacca
     8401 gccgccagta gcactcgcac ggttgttgag gacgttctca acaccgatga ggcgcgggcg
     8461 ctgctcgagg ccggccgggc ctcgggccgg ctcgaggctg ccgagatcgc cctcgcactc
     8521 gacgagctcg acctcgacgc agcgcagatg gacgagttct actccgcgct cgacgaggcg
     8581 cagatcgagg tcgtcgacgg ccaggccgcc gaagctccag cggccgagcc ggagaccgag
     8641 atctccacgg acacgctcca gctgttcctg aaggacatcg gcaaggtgcc cctcctcacc
     8701 gccgctcagg aggtcgagct ggcgaagcgc atcgagcgtg gagagcactc cgccaagcag
     8761 gcgatggtcg aggccaatct gcggctcgtc gtctcgattg cgaagcggta tcgcaaccag
     8821 ggccttccct tcctcgacct catccaggag ggaacgatcg ggttggttcg tgcggccgag
     8881 aagttcgacc accgcaaggg cttcaagttc tcaacctacg ctacctggtg gatccgacag
     8941 gccgtcgcac gggcgatcgc ggacaagggc cggacgatcc ggatgcccgt ccatgtggtc
     9001 gagaagctga acaagatcca tcgctccgag cgaaagctgc gggccgagct ctcgcgggag
     9061 cccaccatcc ccgagatcgc cgccgatctg gagctcgcga tcgaggaggt cgagcagatc
     9121 cgtcggagcg cgcagacacc ggtgtcgctc gagaagccgg tcggcgacga ggaggaatcc
     9181 gagttcgggc atttcctgac ggacgagacg gcgccgctcc ccgacgaggc tgcggaggcg
     9241 gcactccggc gcgaggcgct ccgctcgatc ctcggctcgc tctcgcagcg cgagcgtcag
     9301 gtgctcgagc tccgctacgg actcgacggc cagcagcccc gtaccctcga cgaggtcgga
     9361 aagaccttca acgtcacgcg tgagcggatc cgccagatcg agcaccagag cctgaagaag
     9421 ctgcgggcac tcgcggaggc ctccgcggtt cgcgacgtcg cctgacgaag ccgaacccgc
     9481 tgctcctgat ccccatctag actcgcgcgg gtgatgccgt ctctcgacat gctcgtcgac
     9541 gagctcgccg agctgatcgc gatcccgtcc gtcagcgcgg acgagcagca cgcggccgat
     9601 gtacgccgcg ccgcgaagtg ggtgtgcgac cacattcgcg ccgccggagg cgaggccgac
     9661 gtggtcgacc aggaagggaa gcccctcgtg gtcggcgagc tgcgggcgtc ccggaacgca
     9721 ggcaccgccc cgaccgtgct ctgctacggg cactttgacg tgcagccgcc ggacccgctc
     9781 gatctctggg actccgagcc cttcgagctc acccagcgag acggctggct ctacgcgcga
     9841 ggcgttgccg acgacaaggg acagctcttc atgctcctga aggccgccgg attgctcgcc
     9901 gccgccggag agctgcccgt caacgtccgc ttcgcctgcg acggcgagga ggagacgggc
     9961 ggtcacgcca tcgtcgactg ggcggccgcg gacgagcgcg gcgccgacgt cgcgatcgtg
    10021 ttcgacagct cgatgacgtc gccgggcgtg ctcgagttca acatcgccat gcgcggcatg
    10081 tgctacctcc atctcacgct tcgcacgggc gagcgcgacc ttcattccgg cctgtacgga
    10141 ggcgccgcgc tgaacgcact ccacgccctc gtccgggcgc tgtcgagcgt cctcgccgat
    10201 ccgctgacgg gacgtgtacc cgagccgctc cgggcgggca tcgtccctcc cggctcggcc
    10261 gaggtccggg gatgggagtc gttgcagccc ggggccgaca tgctggcgac gcagggagcg
    10321 cttccgatgg acgagcgcgc agccgaggag ttctacgtcc gtacctgggc cgagccgacc
    10381 gtggaggtga acggcatcga gggcggctca ccgcggctgc agaagacggt tctgcccgtc
    10441 gaggcccgtg cgaacgtctc cctccggctc gctcccgggc agtcacccga cgagatcgtg
    10501 ccggtcttcg agcagctgat ccgcgacgcc ttgcccgcag gcgccgagct ggagctcgag
    10561 cgctgggcga cgggggagcc cacgctcgtc gctccggatc aaccggcgat cgagctcgcc
    10621 cgcgccgcag tcgaggagac ggtcggagtg gctccgctgc tcgtccgctc gggcggcacg
    10681 attcccgtcg tggcgacgct ggccgcccgc gggatcccgg ttgtggtcac cgggttcgcg
    10741 atggtcgact cgcacctcca ttcgcccaac gaacggctga ccgccgagta cctcccgctc
    10801 gggatcgaga ccgcacaggc gctgttccgc cgcttcgagc agcgtgccta gcgccgagta
    10861 cagctcgccg ctcgccgctg agctggccga ggacgtcctc gaccgctttc tccgctacgt
    10921 gcgcatctcg acgcccgcgg tgccggactc ggacacgtat cccagcaatc ctggccagct
    10981 cgatctgtcg cgcctgctcg tggcggagct cgacgaggca ggactcgagg acgtcgagct
    11041 gaccgagcac ggctacgtgt tcgccacgtt gccggggaca gctgcgaatg cccccacggt
    11101 cgggttgatg gcccacgtcg acacggctcc cgacgcgccg ggggacgatg tccggccgat
    11161 cgtgcatcac gcctggcagg gggagaagat cacgcttccg ggcgacccgg cgcagattct
    11221 cgaccccgac gacaaccctg ccctggccga acgggtcggc cacgacatcg tcacctcgga
    11281 cggcacgacg ctgctcggcg ccgacgacaa ggctggtgtg gcggcgatca tggcgggtgt
    11341 cgcccacctc gccgcatccg acgaggcgcg ggcgacggtg cgagtcgcct tcacggtcga
    11401 cgaggagatc ggactgggca ccgagcacct cgacctcgac cacttcgggg ccgacgtcgc
    11461 ttacacgctc gacggctcgg gcctgggcga gctcgaggtc gagacgttct cagcgaagca
    11521 ggtgaccgtc acctttcgag gagtggcggt gcaccccggt atcggcaaag cgcggctggt
    11581 gaacgcgatc aagctggcgt ccgcgttcgt gagctcgctg ccgcaggggg agctgtcgcc
    11641 cgagacgacc gagggtcgcg aagggttcgt ccaccccgtg cgcgtgaccg gcgacgcacg
    11701 caagctgaca gtctccctgc tggcgcgtga ccacgacgag caactgctcg aggaacacgt
    11761 aatccttctg cgccgactcg ccgaggtagc gatcgccgac gagccacgcg cctccgtcga
    11821 cgtcgaggcg gtgacgatgt acaagaacat ggctccggtt ctggagcgtc atcccgaggt
    11881 cgtcggcgca gccgaagagg cgattcgccg tgcgggtgtc gagccggttc acacgatcat
    11941 tcgcggcggc acggacgggg cgcgcctttc cgagcgcgga ctgccgacgc cgaacctgtt
    12001 caccggcggt caggactacc actcgctgcg cgaatgggcc tcggttcagg acatggcagc
    12061 cgccgcggct accgtcgtcg agctcgttcg cgtctggggc gagcgaacca cctaggccgg
    12121 acccgcaggg cgtctagtcg tgctcgtgta cgccgcagcc gagccactcg tgcccgtagc
    12181 tccgcatcgc ttcgatgatc gggagaagcg cgcggccctt ctgggtgagc tcgtactcga
    12241 cgcgtggggg tgactccgcg tagctttggc gcagcacgat ctcctccgcc tcgagccaac
    12301 gcaggcgctc ggccagagtt cgcgggctga tcccgaggca ggagtgctcc agctctgtga
    12361 accgccgtgg cccttccgag aggtcgtgca cgatcaggac agtccacttg gctccgacga
    12421 tcgcggcgca cgcggcgacg ctgcacgtct ccgcctcatg aacgggaagc atcgtgcgcg
    12481 tcgagagcgt atccatcgcc acgacctcat tgtacgtggt ggagcggagc tccggcggtc
    12541 aatcccaggg cggtgccccg tacacccatc ttccacccac catcgtcgcc atgacttcga
    12601 tccccccgag ctcgtccggc gggcagtcga tcggatcgcc gctcagcacg acgaggtcgg
    12661 cgaggaagcc gggcagcagc ctgccgcgac ggcgttcgtc tcccgcgagc cacgccggag
    12721 ccacggtcga ggcttggagc gcctgctcga cggtgagcgc ctgctccggg tgccaggcgg
    12781 gccggtcgtc gttcgtccgg agcacggcag ccccgatccc cgcgagcggg tcgagctcct
    12841 cgaccggcgc gtcggagccg ttggcgacga cggcgcccgc gtcccagagc gacctgtacg
    12901 catacgcgcc ggcgctgacg tcgggccagt accggtcagc gaggtcgcgg tcggccggcg
    12961 catggctgaa ctgaacggag caggcgatgc cgagctccgc gaa
//
LOCUS       DBNGNK010000088        10390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_229149,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000088 DBNGNK010000000
VERSION     DBNGNK010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10390
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_229149"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="AC150V_02330"
     CDS             complement(<1..1463)
                     /locus_tag="AC150V_02330"
                     /inference="COORDINATES: protein motif:HMM:NF013124.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3761789.1"
                     /translation="MSRQLPTGVVTLLFTDVEGSTRLLHELGGDSYGAALAEHRRLLR
                     SFESHGGVEVDTQGDSFFVAFARASDGVAAAADGQRALAGNPISVRMGLHTGEPRLTE
                     EGYVGLDLHKGARIAAVGHGGQVLLSATTQALSEAATRDLGPHRLKDITTPERIYQLE
                     IEGLPSEFPLLKTLEAGITTLPGPTTSFVGRTRELAEIDGLLEDPACRLLTLLGPGGA
                     GKTRLALEAAARRVDRYPHGVHFVSLAPVASPELLAPAVADSLQFSVDAAHSGTPARD
                     QLLDFLAERSTLLVLDNFEHLVEGAELLAQVVERSSKVEIITTSRERLNVRSEWVLDV
                     EGLDEDDEGALRLFVERARQLDSGFALVGKEYEQAARICRLVEGMPLGIELAASWTAM
                     LSCAEIADEIERGLDLLETSARDVPERHRSLRAAFDQSWRLLTDAEREAFARISVFRG
                     GFTREAAAAVAGADLPLLTLLAAKSLVRRQGLGRFDQ"
     gene            complement(1509..2927)
                     /locus_tag="AC150V_02335"
     CDS             complement(1509..2927)
                     /locus_tag="AC150V_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HIR3761790.1"
                     /translation="METIVEFTHGGRPAPPTVAERRAHGKALRKVVPRSSHADWSPPS
                     ARFDPVELIERQNEGRLSWLVPVRHGRMGASPFAFYRGVAQFMAADLASTPVSGLNVQ
                     LCGDAHLSNFGVYGSPERRLVFDINDFDESLAGPWEWDVKRLAASFMIAAQHHDWDPK
                     TCRTLTGRAVQHYRHAMAEFARRRVLDVWYASLPLEELFDATRDEVTKKQRRTRDRLI
                     RKAKSKDSLRALSKLAEKVDGSYRIKSDPPLLIPLRDVPEGVLPSDSLAGFMGAFEEY
                     RSTLPDNRRVLLDRYRPIDFAIKVVGVGSVGTRCFVLLLEGRDEGDPLFLQIKEAGPS
                     VLEGFLEPSPYTQDGQRVVEGQRLMQAASDIFLGWAAGLMKGRQYYFRQLHDMKGSLD
                     VDSADREQLTRYARMCGWTLARAHARTGDPIAIASYLGSGTAFDEALTDFAELYAEQN
                     RRDYDAFTAAIAGGRLDADREG"
     gene            2997..3959
                     /locus_tag="AC150V_02340"
     CDS             2997..3959
                     /locus_tag="AC150V_02340"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3761791.1"
                     /translation="MDLGTSPTVAESTLGELFARMVRIRHFEDEVYRLFLANEVEGTT
                     HLYQGQEAVAVGVADVLQPGDTVAATYRGHGAALALGSDPTRLMAELLGRAGGTNGGR
                     GGSMNVVDLERGLIGCFGIVGGSIAAATGAALAAQLQRSGAIAVAFFGDGATNQAYFH
                     ECLNWASIRRLPVIYACENNLYGEWTAMGRVTAGEGIAERAAAYGIPGHKVDGNDVLV
                     AREAAAAAAARARAGKGPTLLEFQTYRHKGHSKVDPGTYRPKEEVDAWLARDPLALVE
                     SRLDSDEAAGLRADAEREIDAAVAAARSQPFPDSAQRATSTRES"
     gene            3959..4924
                     /locus_tag="AC150V_02345"
     CDS             3959..4924
                     /locus_tag="AC150V_02345"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3761792.1"
                     /translation="MTETSFREAIRDGLAEELERDPNVVLLGEDVAPGGVFNVTPGLS
                     ERFGVDRVIETPISELAFTGAAFGAAVKGLRPVVEIMFGDFLFLVLDTLVNQSSKYWY
                     LSNEQASVPLVVRSAVGAGARLGACHSQAPVGQFLGEPGLKLVAPATAADAKGLTKAA
                     IRDDNPVVVFEHKLLYGKGGDVPEGDAALVPIGKASISRPGSDVTIVAAMAMVDVALA
                     AAEELADEEVDAEVIDLRSLRPLDTETIAESAARTKRVLVIEEGPPTGGYSGDVIATA
                     VEHAGPIAARRVTMPDLPIPFSPTLEDAAVPGVADVVEAAFRLTM"
     gene            4935..6686
                     /locus_tag="AC150V_02350"
     CDS             4935..6686
                     /locus_tag="AC150V_02350"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3761793.1"
                     /translation="MSGTGWLDDAVLRVGLGCMRLSTDEKRDEAGALATIAAAADAGI
                     AVFDTAHAYGRGVHELGHNERLLARALRASDAHERARIVTKGGMARTDGRWVPDGRAK
                     TIRADCEASLAALDGLPIDLYLVHAPDPRTLWRTSVRALARLIDDGVVERVGLSNVNR
                     RQLDEALELAPIAAVQVALNPFDDGALRGGILDRCAELGLTLIAHTPLGGRRRAPALA
                     RNEVLAGIANAHGSTGAEVALAWLLRLSPALVAIPGARRPESARSAARAAGLELADSE
                     LKTLAQEFGAPRPTRPAPSRATSDADVVLVMGIPGAGKSRLAEEYVARGYVRLNRDER
                     GGSLRALAHALDAELSAGVRRIVLDNTYLTRAARSHVIEAAGRHGVPARCTWLDTPLA
                     QAQVHLVERLLDRSGSLPGPDELRQLARREPGLLVPTSQMRTLRELEPPSNDEGFAEI
                     ERVPFARAPARRPRPGGVLLVAGAVGRPGWEGALADTDPATPHLVFDWNPDGSSDDLS
                     AAVTRLVGEVAGPVESALCPHPSGPPRCWCRPPLPGLPLVFARDQSVDLARSTLVGTG
                     PAHRALAETLGARFVQV"
     gene            complement(6743..7144)
                     /locus_tag="AC150V_02355"
     CDS             complement(6743..7144)
                     /locus_tag="AC150V_02355"
                     /inference="COORDINATES: protein motif:HMM:NF039384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6228 family protein"
                     /protein_id="HIR3761794.1"
                     /translation="MPAIEIWSLEGDVGLGFSDMGWEDCFFAFTASLVVDGERASADV
                     SMIAPHAKRMLGYFEEVAQSANGWPGAKVWSSEHRELMLACEDMNREQLCGIATLRLG
                     PTQDEQIAPFIWWRRDVSQIAAHVGARFRQP"
     gene            7303..8100
                     /locus_tag="AC150V_02360"
     CDS             7303..8100
                     /locus_tag="AC150V_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761795.1"
                     /translation="MRRFATWMAGSAFALLVLLALGVAAAGSASMESTVVMKKRYTLT
                     VEYTAFTTEESYLGEQKTGSIERTTSWTAKSVRPFLIERRGTRAKPAYRFFARVAGPL
                     EHRGAGRVAIAATQMPRCWLPGTDSRPDGIVVRAVIKSLSSVPGGAVKLYAKPTQPRP
                     KVVWTTEETTCKLTQAPWQVTIPAHGIPCSPRCVKTPPVWSDHVHKNPGTPQKDIKFG
                     RAFRLERTADSVSDPSLHHETVLPGGARSIVDTTLVWKLTFRPARDR"
     gene            complement(8124..>10390)
                     /locus_tag="AC150V_02365"
     CDS             complement(8124..>10390)
                     /locus_tag="AC150V_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3761796.1"
                     /translation="GGDRRAAHERLSTGALARWTRACATHPWRVVVAWIAIIAGLVVL
                     VAAVGGSLRDEFEIPGSDSQKATDLIEAEFASEQGAVLNLVFAAPVGEQLDTPERRAA
                     IEEAVARLQTDEFAPTEDEAGLASVADPFDPDTVSEDGRIAYAEAQFSETIEDEDREA
                     VLAVQDAVRETVEPAGIVVEFNGDAEFPPVEQGTSEALGLLVAIIVLLLLFRTFVSMF
                     IPIALALTAVGSAFLLLFILAGMTDINTVTPILVSMIGLGVGIDYSLFIVTRFRQLLH
                     EGLSPLDAAAEAGASAGRAVIFAGLTVAISVSGLALFGLDFVTKLGVGSALGVLTTVL
                     IANSLLLAVLALLGHKVDRLKVPFLRQVDDSEAAREKTLIARWGRFVTRNARVVFVVL
                     LLLGLLLASTSALVRLGAADQGTQPTEQTARRAYDLLAEGFGPGFNGPIPIVVDVNGD
                     EQAPQKIFDGVQGLQGVASAREPQLNDAGTVAIVFVTPDSAPQDEETDQLVDRLRSDV
                     VPAATEGGDAVAYVSGVTAAFKDIADRIIERLPFFLLFIIGVTFIVLAMAFRSIVVSA
                     TAAVTTILSAFVGFGVLTLVVQEGYLLGLTGLDRTGPIETFVPPIAFAILFGLSMDYM
                     VFLMSRIREEHVHGLNTKEAVEHGIAAIGRVIVAAAVIMSSVFAAFILSADRIPKEFG
                     LLLAVAILTDALIVRMTLVPAFFTLLGEKTWYIPRWLDRVLPKLTIEPPHDGEPLPAD
                     VGPRAEPAPEPGEA"
BASE COUNT         1622 a         3676 c         3588 g         1504 t
ORIGIN      
        1 tgctggtcga agcgtccgag accctgacgg cggacgagtg acttcgccgc gagcaaggtg
       61 aggagcggca ggtcggcgcc ggcgaccgcc gccgcagcct cgcgcgtgaa tccgccacgg
      121 aagaccgaga tgcgggcgaa cgcttcccgc tcggcgtcgg tgaggagacg ccacgactga
      181 tcgaaggccg cgcgcaggct gcggtgccgc tccggaacgt cgcgcgcaga agtctccagc
      241 aggtcgaggc cacgctcgat ctcgtcggcg atctccgcgc aggagagcat cgccgtccag
      301 ctggcggcga gctcgatacc cagcggcatc ccttcgacca gccggcagat gcgcgccgcc
      361 tgctcgtact ccttacccac gagggcgaag ccggagtcga gctgccgtgc gcgctcgacg
      421 aagagacgca gcgcaccctc gtcgtcctcg tcgagcccct cgacgtcgag cacccactcg
      481 ctccggacgt tgagtcgctc gcgggatgtc gtgatgattt caaccttcga cgagcgttcg
      541 acgacctgcg ccaggagctc ggcgccctcg acgagatgtt cgaagttgtc gagcacaagg
      601 agagtcgagc gctcggcgag gaagtcgagc aactgatccc gagccggtgt gccgctgtgg
      661 gcagcgtcga ccgagaactg aagcgagtcg gcgacggcgg gtgccagcag ctccggagac
      721 gcgacgggcg cgagcgagac gaagtgcacg ccatgcggat agcggtcgac acggcgggcg
      781 gcggcttcga gcgcgaggcg ggtcttccct gccccgcccg gccccaggag cgtgaggagc
      841 cggcacgccg gatcctccag caagccgtcg atctcggcca gctcgcgcgt cctcccgacg
      901 aacgaggtgg tcggaccggg cagcgtcgtg atcccggcct cgagggtctt cagcagtggg
      961 aactcggagg gaagtccttc gatctcgagc tggtagatgc gctcgggggt cgtgatgtcc
     1021 ttgaggcgat gcgggccgag atcgcgcgtc gccgcctcgg acagtgcctg cgtcgtcgcc
     1081 gagagcagca cctggccgcc gtgtccaacc gcggcgatgc gcgccccttt gtgcaggtcg
     1141 aggccgacgt agccctcctc ggtcaaccgc ggctcgcccg tgtgcaagcc catgcgcacg
     1201 ctgatcgggt tgcccgcgag cgcccgctgc ccgtcggcgg ccgccgcgac cccgtccgaa
     1261 gcgcgtgcaa aggcgacgaa gaacgagtcc ccctgggtgt ccacttcgac tccgccgtgc
     1321 gactcgaatg agcggagcaa gcggcggtgc tccgcgagcg ccgcgccgta ggagtcgccg
     1381 ccaagctcgt gaagcagcct cgtcgaccct tcgacgtctg tgaagagaag cgtgacgacg
     1441 ccggtcggca gctgacgcga cacgcggcga tgatcgccgc cccgggaggc gatgtccagc
     1501 gcctcggatc agccctcgcg gtcggcgtcg agcctgccgc cggctatcgc cgccgtgaag
     1561 gcgtcgtagt cgcgcctgtt ctgctccgcg tagagctcgg cgaagtccgt cagcgcctcg
     1621 tcgaaggccg tccccgaccc aaggtagctg gcgatggcga tcgggtcgcc ggtgcgggcg
     1681 tgcgcgcggg cgagcgtcca gccgcacatg cgcgcataac gggtgagctg ctcgcgatcg
     1741 gcgctgtcga catcgagcga gcccttcatg tcgtgcagct gccggaagta gtactggcgg
     1801 cctttcatca gccctgctgc ccaaccgagg aagatgtcgc ttgcagcctg catcaggcgc
     1861 tgcccttcga cgacgcgctg gccgtcctgt gtgtacgggc tcggctcgag gaacccttcg
     1921 agcacggacg gccccgcctc cttgatctgg aggaagagcg gatctccttc gtcgcgaccc
     1981 tcgagcaaca ggacgaaaca gcgcgtgccg acgctgccga cgccgacgac cttgatcgcg
     2041 aagtcgatcg gccgatagcg gtcgagcagg actcgcctgt tgtccggcag cgtcgatcgg
     2101 tactcctcga acgcacccat gaaccccgcg agagaatcgg atgggagcac gccctccggc
     2161 acgtcgcgaa gcgggatcag caacggcggg tcgctcttga tccggtagct gccgtcgacc
     2221 ttctcggcca gcttcgagag cgcccgcagc gagtctttcg acttcgcctt gcggatcaat
     2281 ctgtcgcgtg tccggcgctg cttcttcgtc acctcgtcgc gagtcgcgtc aaagagctcc
     2341 tcgaggggga ggctcgcgta ccagacgtcg agcacgcgcc gacgggcgaa ctcggccatc
     2401 gcatgtcggt agtgctgaac ggcgcgcccg gtgagcgtgc ggcacgtctt gggatcccag
     2461 tcgtggtgct gcgccgcgat catgaagctc gccgcgagcc gcttgacgtc ccattcccac
     2521 ggcccggcaa ggctctcgtc gaagtcgttg atgtcgaaca cgagccgccg ctcgggcgac
     2581 ccgtagacgc cgaagttgga gagatgcgcg tcgccgcaca gctgcacgtt cagtccggag
     2641 accggcgtgg acgccagatc ggcggccatg aactgcgcca ctccgcggta gaaggcaaac
     2701 ggcgacgcgc ccattctccc gtgccgtacc ggtacgagcc aggacaggcg cccctcgttc
     2761 tgccgctcga tcagctcgac gggatcgaag cgagcggacg gcggcgacca gtcggcgtgg
     2821 ctcgagcgag ggacgacctt gcgaagcgcc tttccgtgcg cgcggcgctc ggcgacggtc
     2881 ggcggcgccg gtcgcccgcc gtgcgtgaac tccacaattg tctccatcgt ctcccctcgc
     2941 gcgttgcttc gaccgatctc ccgaccgggg caggcttcgg acactatcct cgcagcgtgg
     3001 atctgggcac cagtccgacg gtcgccgagt cgactctggg cgagctcttc gcacggatgg
     3061 tccgtatccg gcacttcgag gacgaggtct accgcctctt cctcgcgaac gaggtcgagg
     3121 gcacgaccca cctctaccag ggtcaagagg cggtcgccgt cggcgtcgcc gacgtgctgc
     3181 agccgggtga caccgtcgcc gcgacctacc gtggccatgg agccgcgctc gcgctcggct
     3241 ccgacccgac ccgactgatg gccgagctgc tcggtcgcgc gggcggcacg aacggcggtc
     3301 gcggcggatc gatgaacgtc gtcgacctcg agcgcgggtt gatcggctgc ttcggcatcg
     3361 tgggcggctc gatcgccgcg gcgacggggg cggcgctcgc ggcgcagctg cagcggagcg
     3421 gcgcgatcgc ggtcgccttt ttcggcgacg gcgcgacgaa ccaggcgtac ttccacgagt
     3481 gcctgaactg ggcgtcgatc cgcaggctgc cggtgatcta cgcgtgcgag aacaacctgt
     3541 acggcgagtg gactgcgatg ggccgcgtga cggccggcga gggcatcgcg gagcgcgcgg
     3601 ccgcctacgg gataccgggc cacaaggtcg acggcaacga cgtgctcgtc gcgcgggagg
     3661 cggccgcggc ggccgcagcc cgggcacgcg cgggcaaggg cccgacgctg ctcgagttcc
     3721 agacctaccg ccacaagggc cactcgaagg tcgaccccgg cacctaccgg ccaaaggagg
     3781 aggtcgacgc gtggctcgcg cgcgacccgc tggcactggt cgagtcgagg ctcgactcgg
     3841 acgaggcggc cgggctgcgc gccgacgctg agcgggagat cgacgccgcc gtcgccgccg
     3901 cacgatctca gcccttcccg gactcggccc agcgtgccac gtccacgcgg gagtcgtaat
     3961 gaccgagacg agctttcgcg aggcgatccg agacggcttg gccgaggagc tggagcgtga
     4021 tccgaacgtc gtgctgctcg gcgaggacgt cgcgcccggc ggcgtcttca acgtcacacc
     4081 gggcctgagc gagcgcttcg gcgtcgatcg ggtgatcgag acgccgatct cggagctcgc
     4141 attcaccggc gccgcgttcg gggccgccgt caaggggcta cggccggtcg tcgagatcat
     4201 gttcggcgac ttcctcttct tggtgctcga tacgctcgtc aaccagtcct cgaagtactg
     4261 gtacctctcc aacgagcagg ccagcgtccc gctcgtcgtg cggagcgccg tcggagcagg
     4321 agcgaggctg ggcgcgtgcc actcgcaggc gcccgtgggc cagttcctcg gcgagccggg
     4381 tctcaagctc gtcgcgcccg ccaccgccgc cgacgcaaaa ggcctgacga aagcggcgat
     4441 ccgtgacgac aaccccgtcg tcgtcttcga gcacaagctg ctctacggca agggcggcga
     4501 cgtgccggaa ggcgacgccg cgctcgtccc gatcgggaag gcatcgatca gccggcccgg
     4561 gtcggacgtg acgatcgtcg cggcgatggc gatggtcgac gtcgcgctcg ccgccgccga
     4621 ggagctcgcc gacgaggagg tcgacgccga ggtgatcgac ctccggtcgc tgaggcccct
     4681 cgacaccgag acgatcgcgg agtcggccgc gcgcacgaag cgcgtcctcg tcatcgagga
     4741 ggggccgcct acgggtggct actcgggcga tgtaatcgcg acggccgtcg agcacgccgg
     4801 cccgatcgca gcacgccgcg tgacgatgcc ggacctgccg atccccttca gccccacgct
     4861 cgaagacgcc gccgtcccgg gagttgccga cgtcgtcgag gccgcgttcc gactcacgat
     4921 gtagggcctt catcgtgtcc ggcactggtt ggctcgacga cgcggtgctc cgcgtcggcc
     4981 tcgggtgcat gcgcctttcg accgacgaaa agcgcgacga ggcgggcgcc ctcgcgacga
     5041 tcgccgccgc tgccgacgcc ggcatcgccg tcttcgacac cgcgcacgca tacggccgcg
     5101 gcgtccacga actcggccac aacgagaggc tgctcgcccg agctctgcgc gcgagcgacg
     5161 cgcacgaacg cgcacgcatc gtgacgaagg gaggcatggc gcgaaccgat gggaggtggg
     5221 tgcccgacgg tcgagcgaag acgattcgcg cagattgcga ggcgagcctc gccgctctcg
     5281 acgggctgcc gatcgacctc tacctcgtcc acgcgccgga tccgcggacg ctctggcgga
     5341 catcggtgcg agccctggcc cggctgatcg acgacggtgt cgtcgagcgg gtcggtctgt
     5401 cgaacgtaaa ccgacgccag ctcgacgagg ctctcgagct cgccccgatc gcggcagtcc
     5461 aggtcgcact caaccccttc gacgacggtg cgctacgcgg tggcatcctc gatcgctgcg
     5521 ccgagctcgg actcaccctg atcgcccaca ctccgctcgg cggtcggcga cgggctccgg
     5581 ccctcgcccg caacgaggtg ctggccggca ttgcgaacgc acacggttcg accggggccg
     5641 aggtcgcgct cgcgtggctg ctccgcttgt cccccgctct ggtagcgatc cccggtgcga
     5701 ggcgtcccga gtcggcgcgc tcggcagcac gggccgccgg cctcgagctc gccgactccg
     5761 agctgaagac gctcgcccag gaattcggtg cgccgcggcc gactcgcccc gcaccgtcgc
     5821 gtgcgacgtc cgacgccgac gtcgtcctcg tgatggggat cccgggagcc ggcaagagcc
     5881 gccttgcaga ggagtacgtc gcccgcgggt acgtccgcct caaccgtgac gagcggggcg
     5941 gatcgctccg agcgctcgcc cacgcgctcg acgccgagct gtcggcagga gtacggcgga
     6001 ttgtcctcga caacacgtac ctcacgcggg ccgcacgcag ccacgtgatc gaggcggcgg
     6061 gccggcacgg cgtgccggca cgatgcacct ggctcgacac tcccctcgcg caggcgcagg
     6121 tacacctcgt cgagcggctg ctcgaccgct ccggctccct ccccggtccc gacgagctgc
     6181 ggcagctggc gcggcgggag cccggactgc tcgttccgac ctcgcagatg cgcactctgc
     6241 gcgagctcga gccgccatcg aacgacgagg ggttcgctga gatagagcgc gttccgttcg
     6301 ctcgcgcgcc tgcacgacga ccgcgtccgg gtggcgtcct cctcgtcgcc ggggcggtcg
     6361 ggcgtcccgg ctgggaaggc gcgctggccg acaccgaccc cgcgacgccg catctcgttt
     6421 tcgactggaa cccggacggc agctcggatg acctgtcggc cgcggtcaca cgcctcgtgg
     6481 gcgaggtcgc cggacccgtc gagagcgcgc tgtgtccgca cccgtcgggc ccgccgcgct
     6541 gctggtgccg tccgccgctt ccgggactgc ctctggtctt cgcccgggat cagagcgtcg
     6601 atctcgcgcg ctcgacgctc gtcggcaccg ggcccgcgca ccgcgcgctc gccgagacgc
     6661 tcggagcccg attcgtgcag gtctgagccc ccactgcagg aacgcggcag cttcgccgca
     6721 tccacatagc cgaccgtacg ccctacggct gacgaaagcg cgcgccgacg tgtgcggcga
     6781 tctggctcac gtcgcggcgc caccagatga acggggcgat ctgctcgtcc tgcgtcggcc
     6841 ccagacgcag cgtcgcgatg ccgcagagct gctccctgtt catgtcctcg caggcgagca
     6901 tcaactcgcg gtgctccgag ctccagacct tcgcgcccgg ccatccgttg gcgctctggg
     6961 ccacctcctc gaagtagccg agcatgcgct tcgcgtgcgg ggcgatcatc gacacgtcgg
     7021 cagatgcgcg ttccccgtcg acgaccagcg aggccgtgaa cgcgaagaag cagtcctccc
     7081 agcccatgtc cgagaacccg agccccacgt ctccctccag cgaccagatc tcgatcgcgg
     7141 gcatgtgtcg cgtctcctcg tccttgaagc gctctttctc tacgtcggcc gtagagcaaa
     7201 agactcaacc ccgaacagcg aactgccgga tcggattcag cggcctcccc gatgccgccg
     7261 atgccgtggt ggcggacact ggagtcgaga ggaggcgatc tcgtgaggcg cttcgcgacg
     7321 tggatggcgg gttcggcgtt cgcgctcctc gtgctcctcg ccctcggagt cgccgcagcg
     7381 ggaagcgcgt cgatggagtc gacggtcgtg atgaagaagc gctacacgct gaccgtcgag
     7441 tacaccgcct tcaccaccga ggagtcgtac ctcggcgagc agaagacggg gagcatcgag
     7501 cgcacgacca gctggactgc gaagagcgtg cggccgttcc tgatcgagcg tcgcggcacc
     7561 cgggccaagc ccgcgtaccg gttcttcgcc cgcgtcgccg gcccgctcga gcaccggggt
     7621 gccggcaggg tggcaatcgc ggcgacgcag atgccccggt gctggctccc gggcactgac
     7681 tcacgccccg acgggatcgt cgtccgtgcg gtgatcaagt cgctctcgtc ggttccgggc
     7741 ggcgccgtca agctctacgc caaaccgacg cagcccaggc cgaaggtcgt ctggacgacc
     7801 gaggagacga cgtgcaagct cacgcaggcg ccgtggcagg tcacgatccc ggcccacggc
     7861 atcccgtgct cgccacgctg cgtgaagact ccacccgtct ggagcgacca cgtgcacaag
     7921 aacccgggca cgcctcagaa ggacatcaag ttcgggcgcg ccttccggct ggagcgcacc
     7981 gccgattccg tttcagaccc gagcctgcac cacgagacgg tcctccccgg cggggcccgg
     8041 tcgatcgtcg acacgacctt ggtctggaag ctgacgttcc ggcccgcgcg agaccgctag
     8101 tccggtggag ccgacgtgag gcgtcaggcc tcgcccggct cgggcgccgg ctcggcccga
     8161 ggccccacat cggcgggcaa cggctcgccg tcgtgcggcg gctcgatcgt gagcttcggc
     8221 agcacgcggt cgagccatct cgggatgtac cacgtcttct cgcccaagag cgtgaagaac
     8281 gcgggcacga gcgtcatccg cacgatcagc gcgtccgtca ggatcgcgac cgcgagcagc
     8341 aggccgaact ccttcgggat gcggtcggcg ctcaggatga acgctgcgaa gacggagctc
     8401 atgatcaccg ccgcggcgac gatgacgcgc ccgatcgcgg cgatgccgtg ctccacggcc
     8461 tccttcgtgt tcaggccgtg cacgtgctcc tcccggatgc ggctcatcag gaagaccatg
     8521 tagtccatgg acaatccgaa caggatcgcg aaggcgatcg gcggcacgaa agtctcgatc
     8581 gggccggtgc ggtcgagccc ggtcaggccg agcaggtagc cctcctgcac caccagcgtg
     8641 agcacgccga agccgacgaa ggcggacagg atcgtggtga ccgccgcggt ggccgatacg
     8701 acgatcgacc ggaaggccat cgcgagcacg atgaaggtga cgccgatgat gaacaacagg
     8761 aagaacggca ggcgctcgat gatccggtcg gcgatgtcct tgaaagctgc ggtcacgcct
     8821 gagacgtagg cgaccgcgtc gccaccctcg gtggcggcgg gcaccacgtc gctgcggagg
     8881 cggtcgacga gctgatcggt ctcctcgtcc tgcggcgccg agtcgggagt gacgaagacg
     8941 atcgccacgg tgcctgcgtc gttcagctgc ggctcgcggg cggaggcaac gccctgcagg
     9001 ccctgcacgc cgtcgaagat cttctgcggc gcctgctcgt cgccgttcac gtcgaccacg
     9061 atcgggatcg ggccgttgaa gccggggccg aagccttccg ccaacaggtc gtaggccctg
     9121 cgggcagtct gctcggtcgg ctgcgtcccc tggtccgccg ccccgagtcg caccaatgcc
     9181 gaggtcgacg cgagcaagag cccgagcagc agcaggacga cgaacacaac gcgggcgttc
     9241 ctcgtcacga atcttcccca gcgggcgatg agcgtcttct cgcgtgccgc ctccgagtcg
     9301 tcgacctggc gcaggaacgg cactttcagc cggtcgacct tgtgcccgag gagcgccagc
     9361 accgccagca gcaaggagtt cgcgatcagc acggtcgtga gcacgccgag ggcgctgccg
     9421 acgccgagct tcgtcacgaa gtcgagcccg aagagcgcca gcccgctcac tgagatcgcc
     9481 acggtcaggc ccgcgaagat caccgcgcgg cctgccgacg ccccggcttc cgccgccgcg
     9541 tccagcggag acaggccctc gtgcagcagc tgtctgaaac gggtgacgat gaacagcgag
     9601 tagtcgatgc ccacccccag gccgatcatc gagacgagga tcggcgtcac ggtgttgatg
     9661 tcggtcatcc ccgcgaggat gaagagcagc aggaaggcgc tccccaccgc cgtcagcgcg
     9721 agcgcgatcg ggatgaacat cgacacgaac gtgcggaaca ggagcagcag cacgatgatc
     9781 gccacgagca ggccgagcgc ctcggacgtc ccctgctcga ccggcgggaa ctcggcgtcg
     9841 ccgttgaact cgacgacgat gcccgcaggc tcgaccgtct cgcgcacggc gtcctgaacc
     9901 gccagcacgg cttcgcggtc ctcgtcctcg atcgtctccg agaactgcgc ctccgcgtag
     9961 gcgatgcgcc cgtcctccga gaccgtgtcc ggatcgaacg ggtcggccac gctcgccagg
    10021 cccgcctcgt cctcggtcgg cgcgaactcg tccgtctgca ggcgggcgac cgcctcctcg
    10081 atcgcggcgc ggcgctcggg agtatcgagc tgctcgccga cgggcgcggc gaagacgagg
    10141 ttgaggacgg ccccctgctc cgaggcgaac tcggcctcga tcaggtctgt cgccttctgc
    10201 gagtccgagc cgggaatctc gaactcgtcc cgcaacgagc cgccgacggc ggccacgagc
    10261 acgacgagtc cggcgatgat ggcgatccag gccactacca cgcgccaggg gtgcgtggca
    10321 caggcgcgcg tccagcgcgc gagcgcgcct gtcgagaggc gctcgtgcgc tgcgcgtcgg
    10381 tctccgccag
//
LOCUS       DBNGNK010000089         7033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1661129,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000089 DBNGNK010000000
VERSION     DBNGNK010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7033
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1661129"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="AC150V_02370"
     CDS             <1..502
                     /locus_tag="AC150V_02370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR3761797.1"
                     /translation="IGPPWEVSPPGVPAVVIDPGLAFGTGAHPTTRLCIELLATLEGG
                     SVLDVGCGSGVLAIAAATLGFEPVWAVDVEPVAVNVALANAVANGVTVDASVADARIA
                     ALPVVDITLANIALGEVEELLRRLDSRRVIASGYVVGERPGAAGWTLVARRGLDGWAA
                     DLFARK"
     gene            516..1712
                     /locus_tag="AC150V_02375"
     CDS             516..1712
                     /locus_tag="AC150V_02375"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR3761798.1"
                     /translation="MATFTASFLGCKVSFADTQSIRERLLSDGHVPSDGPGDVAVVNT
                     CCVTNEAVSKSRQAVARAAREHSRVYVTGCAANLPGEPFGGLAANVVVVPGRSEDAVE
                     RVAGDVGAIGCVQTDQRLDRVRAFVRIQDGCSFSCRFCVIPLVRGATRSRSAAAVLAE
                     VARRAGQGHREVVLTGVNLGCFRDRKAGYTLARLVRDAARVTGIERLRLSSIEVNHLT
                     EELVAALRETPGVSRHLHVPLQSGDDRVLAAMGRRYTVSTFLRKVSPLTDFNVTSDVI
                     VGFPAEDASAFENTLRVVEQARITKAHVFPYSPRPGTVTEKSDPVPADVKKERSARLR
                     AASEAACRTRWLEKIGGEDLVLVDRPGRGYGDDYSPWLVDAPVGRLVRARAVGVSEEG
                     VLGAAA"
     gene            1675..2031
                     /locus_tag="AC150V_02380"
     CDS             1675..2031
                     /locus_tag="AC150V_02380"
                     /inference="COORDINATES: protein motif:HMM:NF023395.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HIR3761799.1"
                     /translation="MSARKGCSALPPDCLFCSLVADGDHVHAADGFVAIRDVAPKAPT
                     HLLVLPERHVDSFRDVARFSDEEAGRMLRFVAETARLAGLDDYRVIVNVGPGGGQTVF
                     HLHWHVLGGDSLPEFE"
     gene            2028..2471
                     /locus_tag="AC150V_02385"
     CDS             2028..2471
                     /locus_tag="AC150V_02385"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR3761800.1"
                     /translation="MSLIARIEEELKQARLARDSDLRNVLSLILASLRSAEKELQRPL
                     HDDEELQVLQRERKKRAEAIEAYRAAGREEQADAEEYELSVLEDFMPEPLSEDELEEI
                     VDDVIAEVGATSIRDLGRVMADVMPQVSGRADGSTVSQLVREKLA"
     gene            2725..3684
                     /locus_tag="AC150V_02390"
     CDS             2725..3684
                     /locus_tag="AC150V_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019855020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR3761801.1"
                     /translation="MQDVLVVPNEVAAELAGVGDGVLDTLRDRLGCAIALRGNRLTLD
                     GEKLHVAEARAVLDELVELVEGGHQIGPDTVGAVLAALDQAADIRDVFDDVVWRHRGK
                     KIAPKTVTQKHYVDAIRSRTVTFGVGPAGTGKTYLAMALAVAALQEKQVGRLILTRPA
                     VEAGERLGFLPGDMLAKVDPYLRPLFDALYDMLDADRLAAYMDRGTVEVAPLAFMRGR
                     TLNDSFIILDEAQNTTPEQMQMFLTRLGFGSKVVVTGDITQVDLPREQASGLIQVREI
                     LAGVDGIAFVEFGHEDVVRHALVQRIVEAYKRHAEETGTARRT"
     gene            3745..4071
                     /gene="ybeY"
                     /locus_tag="AC150V_02395"
     CDS             3745..4071
                     /gene="ybeY"
                     /locus_tag="AC150V_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR3761802.1"
                     /translation="MLALARSVLAGEGVEAGELGLAFVTAEEIRALKGEHLGIDEPTD
                     VLSFPLDGTAALPEGVPRALGDVVLCPEVVGAAWRAPLVHGLLHLLGYEHGREMDARE
                     RAYAET"
     gene            4071..4802
                     /locus_tag="AC150V_02400"
     CDS             4071..4802
                     /locus_tag="AC150V_02400"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR3761803.1"
                     /translation="MSPPRGRRAPTLFESFNHALEGLIHVLRTQRNMRIHFAISTGVL
                     VAALLFDVTRLELIALLLAIAFVLIAEMINSALEAAVDVASTSFDPMAKLAKDIAAGA
                     VLIASITAVGIGYLVFSGEVADRSSRFLDRLSETPAELTLVALVFTVFLVIATKALTG
                     RGTPLRGGLPSGHAAVAFAVWMAITLTLDDTSHRFLISSLAFIMALLVAQTRIESGIH
                     SAVEVLYGGVLGALVTLVIFQVFSA"
     gene            4799..5173
                     /gene="cdd"
                     /locus_tag="AC150V_02405"
     CDS             4799..5173
                     /gene="cdd"
                     /locus_tag="AC150V_02405"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012337201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR3761804.1"
                     /translation="MTDDELLGRADAAAERAYAPYSRFHVGCAVLARDGGVIEGANVE
                     NAAYPLGVCAERTALSRAIAEGYRPGDFATVAITASPCGGCRQWLAEMEVERVVFRNG
                     GRVVTMTVDELLPEQFDSSDLT"
     gene            5170..6024
                     /gene="era"
                     /locus_tag="AC150V_02410"
     CDS             5170..6024
                     /gene="era"
                     /locus_tag="AC150V_02410"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR3761805.1"
                     /translation="MKSGFVVVAGRPNVGKSTLVNALCGDKVAITSRVPNTTRRRIFG
                     VANGPGWQLVLADLPGFQRPMDPLTERMQATVDASFDDVDVVLLVVSARDRIGAGDRF
                     VARRVFSLGVPVVIALNKVDRLKASHIASQMKQAATLGDFHALHPVSAKTREGVAELR
                     GDLVHLLPAGPAFFPPDQATDLTIAERVTELIREKALQLTREEVPHALTVELDEMDER
                     RIRAFVLVETESQKGILVGKRGATIREIGTRARPEVEQLVGHPVYLELVVKVRPKWRR
                     DPKQLERL"
     gene            complement(6203..>7033)
                     /locus_tag="AC150V_02415"
     CDS             complement(6203..>7033)
                     /locus_tag="AC150V_02415"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3761806.1"
                     /translation="MELAAIAERFDTPALHGRAAVAQGTLSLAREDLAEAERSLRSGR
                     ELLREAGVVLESAEAGVLLGRARIAAGDAAAGTRTIEEAAERLERLGAHPAAARAREL
                     LLEQQPVSERVVRAFVFTDICSSTDLVAAIGDDDWRELVEWHDRALRECVREHGGEEV
                     KALGDGFFITFADPATALACATSMQRRLAEHRRQHGFAPRVRIGVHLAEAVAAAGDYR
                     GRGVNEAARIGALAGPDEVLASSATIAAAGGGVKTSEPRTVDLKGFPDPVDVVTVVWS
                     "
BASE COUNT         1042 a         2404 c         2437 g         1150 t
ORIGIN      
        1 gatcggtccg ccgtgggagg tgtcgccgcc cggcgtaccg gcggtggtca tcgatccggg
       61 cctggcgttc ggaacgggtg ctcatccgac gactcgtctg tgcatcgagc tcctggctac
      121 gctcgaaggg gggagcgttc tcgacgtcgg ctgtggatcc ggtgtgctgg cgattgccgc
      181 ggccacgctc ggcttcgagc cggtttgggc agtcgacgtc gagcccgtcg ccgtcaacgt
      241 ggctctggcg aatgcagtcg ccaacggggt aaccgttgac gcctcggtcg cggacgcgag
      301 gattgcggcg ttgccggtcg tcgacatcac gctggccaac atcgcgctgg gagaggtcga
      361 ggaactcctg agacggctcg attcgcgtcg cgtgatcgca tcgggttatg tggtgggcga
      421 gcggccgggc gccgcgggct ggacgctcgt cgcgcgtcgc ggactggacg gctgggcagc
      481 cgatctgttc gcgcggaagt agcattccgg cggcgatggc cacgttcacg gcgagcttcc
      541 tcggctgcaa ggtgtcgttc gccgacacac agtcgattcg cgagcgcctt ctttcggacg
      601 gtcacgttcc gtcagacggc cctggcgatg tcgcggtcgt gaacacgtgc tgtgtcacca
      661 acgaggcggt ttcgaagtcg cgtcaggccg tcgctcgtgc ggccagggag cactcccgcg
      721 tctacgtgac gggctgcgcg gccaatcttc ccggcgagcc gttcggcggc ctagcggcga
      781 acgtcgtcgt cgtgcccggc cggagcgagg acgcggtcga gcgagttgcc ggcgacgtcg
      841 gcgcgatcgg ctgcgtccag acggatcagc ggctcgatcg ggtccgtgcg ttcgtgcgca
      901 tccaggacgg atgtagcttc tcgtgtcggt tctgcgtgat cccactcgtc cgcggtgcta
      961 cccgtagccg atctgcggcg gcggtgctcg cggaggtcgc acgtcgcgcc gggcaaggac
     1021 accgtgaagt cgtcctcacc ggcgtcaacc tcggttgctt tcgagaccgg aaggccggct
     1081 acacgcttgc gcgactcgtg cgcgatgctg ctcgagtcac cggcatcgag cggcttcgtc
     1141 tctcctcgat cgaggtcaat cacctgaccg aagagctcgt ggcggcgctc cgcgaaacac
     1201 ccggagtgtc caggcatctc cacgtcccac tccagtcggg cgacgaccgg gtgctcgctg
     1261 cgatgggccg tcgctacacg gtcagcacgt tcctgcgcaa ggtctccccg ctcacggact
     1321 tcaacgtcac gagcgacgtg atcgtgggct tccccgcaga ggacgcgtcg gcgttcgaga
     1381 acacgcttcg cgtcgtcgag caggcacgca tcacgaaagc ccacgtcttt ccgtactcgc
     1441 cgcgccccgg aactgtcacc gagaagagcg atccggttcc cgccgacgtg aagaaggagc
     1501 ggagtgcgcg cctgcgcgca gcgtccgaag ccgcgtgccg cacacgctgg ctggaaaaga
     1561 tcggaggcga ggatctcgtt ctcgtcgacc ggcccggtcg aggctatgga gacgactaca
     1621 gcccatggct ggtcgacgca cccgtgggcc ggcttgtccg ggcccgcgcg gtgggtgtca
     1681 gcgaggaagg ggtgctcggc gctgccgcct gactgcctct tctgctcgct cgtcgccgac
     1741 ggtgaccatg tccacgcagc cgacgggttc gtggcgattc gcgacgtcgc ccccaaggcg
     1801 ccgacgcatc tcctggtcct gccggaacgg cacgtcgact cctttcgcga cgttgcccgt
     1861 ttctctgacg aggaggcggg ccgaatgctc cgcttcgtcg ccgagaccgc ccgcctggcg
     1921 gggctcgatg actaccgcgt catcgtcaac gtcggccccg gaggcggcca gacagtgttt
     1981 catctgcact ggcacgtgct cgggggcgat agcctgccgg agttcgaatg agcctgatcg
     2041 cacgaatcga agaagaactc aaacaagcgc gcctcgcgcg cgacagcgac ctgcgcaacg
     2101 tgctgtcgct gatacttgcc agtctccggt cggcagagaa ggagttgcag cgcccacttc
     2161 acgacgacga ggagcttcag gtgctgcagc gcgagcggaa gaagcgcgca gaggcgatcg
     2221 aggcctaccg tgcggcaggg cgagaagagc aggccgacgc ggaggaatac gagctctcgg
     2281 tgctcgagga cttcatgccc gagccgctga gcgaggacga gctcgaggag atcgtggacg
     2341 atgtcatcgc ggaggtaggc gctacgtcga ttcgcgacct cggccgcgtg atggcggacg
     2401 tgatgccgca ggtctccggc cgcgccgacg ggtcgacggt cagccagctg gtacgggaga
     2461 agcttgcgta gacaccgtcc gccgcgtcgg ttgcccgaac cgtcacgaga tgcgacctcg
     2521 gctggtcgac ggggctcggc gcgttggcgc cgtcgatgcg aacggacacc ccccgtgggg
     2581 gagcagacgc ccccagatgc acgctagctc gcggcgtgca acggatctga gtccggcctg
     2641 tgccgaatct gtgccggtcg gattccgcgc ccccaactcg tccggccgca gagcggctcg
     2701 cggggagttc ctacactgag ctcagtgcag gacgtcctgg tcgttcccaa cgaagtggcc
     2761 gccgagctgg cgggcgtggg cgacggcgtc ctcgacacgc tgcgtgaccg cctcgggtgc
     2821 gcgatcgcgc ttcgcggcaa ccggctcaca ctcgacgggg agaagctcca cgtcgctgag
     2881 gcgcgcgccg tcctcgacga gctcgtcgag ctggtggaag ggggtcacca gatagggccg
     2941 gacacggtcg gggcggtact cgcagcgctc gaccaggcgg cggacatccg cgacgtcttc
     3001 gacgacgtcg tctggcggca tcgcgggaag aagatcgccc ccaagacggt gacacagaag
     3061 cactacgtag acgccattcg ctcgcgcacg gtcacgttcg gcgtcgggcc ggcaggaacg
     3121 gggaagacgt acctggcgat ggcgctagcc gttgcggcgc tacaggagaa acaggtcggc
     3181 cggctcatcc tcacccgccc ggcggtcgag gccggagaac ggctcggctt ccttcccggt
     3241 gacatgctcg ccaaggtcga cccctacctc cggccgctct tcgacgccct ctacgacatg
     3301 ctcgacgccg acaggctcgc cgcctacatg gatcgcggca ccgtcgaggt ggcaccgctc
     3361 gccttcatgc gaggcaggac gctcaacgac tccttcatca tcctggacga ggcacagaac
     3421 acgacgccgg agcagatgca gatgttcctc actcggctcg gcttcggctc gaaggtcgtg
     3481 gtgaccgggg acatcacgca ggtcgacctc ccgcgagagc aggcgtcggg gctgatccag
     3541 gttcgcgaga tcctcgccgg cgtcgacggc atcgccttcg tcgagttcgg ccacgaggac
     3601 gtcgtccggc acgcgctcgt gcagcggatc gtcgaggcct acaagcgcca cgcggaggag
     3661 accggcaccg cacgccgcac gtagagggcg ttggtcgagg tcgagctaga caaccgcagt
     3721 ggatccccgg tcgaggaggc cgaggtgctc gcactcgccc ggagcgtgct cgccggagag
     3781 ggagtcgagg cgggcgagct cgggctggcc ttcgtgaccg cggaggagat acgcgcgctc
     3841 aaaggtgagc atctgggcat cgacgagccg acggacgtgc tctcgttccc gctcgacggc
     3901 acagccgcgc tgccggaggg cgtgccgcgt gcgctcggcg acgtcgtgct ctgtcctgag
     3961 gtcgtcggcg ccgcctggcg ggcgccgctc gtgcacgggc tgctgcacct tctcggctac
     4021 gagcacggcc gtgagatgga cgcccgcgaa cgggcgtacg cggaaacgtg atgtctcctc
     4081 cgcgcggacg ccgggcgccg acactcttcg agagcttcaa ccacgcgctc gagggcctca
     4141 tccacgtcct gcgcacacag cggaacatgc gcatccactt cgccatttcg accggcgtgc
     4201 tggttgcggc actcctcttc gacgtgaccc gtctcgagct gatcgctctc ctcctggcga
     4261 tcgccttcgt cctgatcgcc gagatgatca actcggcgct cgaggctgcc gtcgacgtcg
     4321 cgagcacctc gttcgatccg atggccaagc tggcgaagga catcgccgcg ggagcggtgc
     4381 tgatcgcttc gatcaccgcc gtcggcatcg gctatctcgt cttttcgggc gaggtggccg
     4441 atcgcagctc acggttcctc gaccgcctct cggagactcc ggcggagttg acgctcgtgg
     4501 ccctcgtctt caccgtcttc cttgttatcg cgacgaaggc gttaaccgga cggggcacgc
     4561 cgctgcgcgg cggtttgccc tcgggacatg ccgccgtggc cttcgccgtg tggatggcga
     4621 tcacgctgac gctcgacgac acgtcgcacc gcttcctgat ctcctcgctg gcgttcatca
     4681 tggctctgct ggtggcgcag acgcgaatcg agtccggcat ccactcggcc gtcgaggtcc
     4741 tctacggggg cgtactcggt gccctcgtga cgctcgtgat cttccaggtg ttctcggcat
     4801 gaccgacgac gagctgctgg gccgcgccga cgccgccgcg gaacgggcgt acgcgccgta
     4861 ctcgcgcttt cacgtcggct gcgccgtgct cgcgcgtgac ggcggcgtga tcgagggcgc
     4921 caacgtcgag aacgccgcct acccgctggg cgtctgcgcg gaacgaacgg cgttgtcgcg
     4981 cgcaatcgcg gagggctacc gcccgggcga cttcgcaacc gtcgcgatca ccgcctcgcc
     5041 ctgcggcggc tgccgccagt ggctcgcgga gatggaggtc gagcgggtgg tgttccgaaa
     5101 cggcggcagg gtcgtgacga tgacggtcga cgagctgctg cccgagcagt tcgactcgtc
     5161 cgacctcacg tgaagtccgg cttcgtcgtc gtcgcgggcc gccccaatgt cggcaagtcg
     5221 acgctcgtca acgcgctctg cggcgacaag gtggcgatca cgtcgcgcgt gccgaacacc
     5281 acgcggcgcc ggatcttcgg cgtcgcgaac ggcccggggt ggcagctggt actcgccgat
     5341 ctgcccggct tccagcgccc gatggatccg ctgacggagc ggatgcaggc gactgtggac
     5401 gcctccttcg acgacgtcga cgtcgtgctg ctcgtcgtct cggcacgcga ccggatcggt
     5461 gccggcgacc gcttcgtcgc ccggcgcgtc ttctcgctcg gcgtcccggt cgtgatcgcc
     5521 ctcaacaagg tcgaccggct gaaagcgagt cacatcgcaa gccagatgaa gcaggccgcg
     5581 actctcggcg actttcacgc gctccatccc gtcagcgcga agacccgcga gggcgtcgcc
     5641 gagctgcgcg gcgacctcgt tcacctcctg ccggcggggc cggccttctt cccgcccgac
     5701 caggccaccg acttgacaat cgccgagcgc gtcaccgagc tgatccgtga gaaggcgctc
     5761 cagctgacgc gcgaggaggt tccgcacgcg ctgacggtcg agctcgacga gatggatgag
     5821 cggcggatcc gcgccttcgt gctggtcgag acggagtccc agaagggcat tctcgttggg
     5881 aagcggggcg ctacgattcg tgagatcggc acgcgcgcgc ggcctgaggt ggagcagctc
     5941 gtcggccatc ctgtctacct cgagctcgtc gtcaaggtgc gcccgaagtg gcgtcgcgat
     6001 cccaagcagc tcgagcgact ctgactctga ctctgactct gactgcagac cgctgatggc
     6061 gcggcgccgc gggctagcgc cgtgctgacc tgccccgact gcggccagga gagccccgag
     6121 ggttttcgtc tctgcccagg cctgcgcctc ggccgcggcg agcagctcct cgccgacacg
     6181 gcctgacgcg cccccacagc ggctatgacc atacgacggt cacgacgtcg accgggtcag
     6241 gaaagccttt taggtcgacg gtcctcggct cagaggtctt tacgccgccg ccggcggccg
     6301 cgatcgtcgc ggagctggcg agaacctcgt caggcccggc gagcgctccg atccgcgcag
     6361 cctcgttgac gccgcggcct cggtagtcgc cggccgcggc gactgcctcg gccagatgga
     6421 cgccgatccg aacacgcggc gcgaagccgt gctgtcgccg gtgctcggca agacgacgct
     6481 gcatcgaggt ggcgcaggcc agcgcggtcg ccgggtcggc gaaggtgatg aagaagccgt
     6541 cacccagtgc ctttacctct tcgccgccgt gttcgcgcac acactcgcgg agcgctcggt
     6601 cgtgccactc gacgagctcg cgccagtcgt cgtcgccgat cgcggcgacg agatccgtcg
     6661 acgagcagat gtccgtgaag acgaaggcgc gcacgacgcg ctcgctcacc ggctgctgct
     6721 cgagcagcag ctcgcgtgcc cgtgcagctg ccgggtgcgc gccgagtcgc tcgaggcgct
     6781 ctgcggcctc ctcgatcgtg cgggttcccg ccgccgcgtc accggctgcg atgcgcgccc
     6841 ggccgaggag cactcccgcc tcggccgact ccagtacgac gcctgcctcc cgcagcagct
     6901 ccctgccgct ccggaggctc cgctcggcct cggcgaggtc ctcgcgggcg agcgaaagcg
     6961 ttccctgcgc gacggctgcg cgcccgtgca acgccggtgt gtcgaagcgc tcggcgatcg
     7021 cggccagttc cat
//
LOCUS       DBNGNK010000090         7095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1734107,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000090 DBNGNK010000000
VERSION     DBNGNK010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1734107"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3221
                     /locus_tag="AC150V_02420"
     CDS             <1..3221
                     /locus_tag="AC150V_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3761807.1"
                     /translation="GDAILLGAEPDLAPITPPFWDPAGIELAHLLPAEYTLELRVEPA
                     DPEHRHVAVAFVEIGGTDSLLARDGAGALAEALDERVSGVQEACLRFGVTFAQTDVSA
                     GGTKAILLAGAPRSAGGDEEELLLRAVRAIVETPAELPVRAGINSGRVFAGVVGPATR
                     RTYTFYGDAINTAARIMARAEPGQLLARDEVLERARTTYDITPIEPFSAKGKAEPVHA
                     SAVGPATGERQLEATGPLVGRGNELATLLEALDGARAGTGGLVILRGEAGLGKTRLLL
                     ELRERAEGARIVAVHCEQVEQSRPYATAGAILRRALHVDLHSPAREVEARLRFEVGGR
                     APELEPWLPLLGIPLGLDLPQTPETSRLDEKFVPERIAEMVDAILQTLVPDAALVVID
                     DAHWLDEASNELIGRLARGLAERPWLIAAARRNLAGGLRTPDSIESTAVDLEPLSSDE
                     AKRLVVELTADAPVGDHVADAVARRSAGSPLFVTELVTAVRAGADVGHLPESVEALMA
                     MQIDELPAGDRAALRQASVLGARFELETLLATLELEPAEGLALLQRLQAFLFLDDDGL
                     VRFRHGLVRESAYEGLPYRRRRQLHRRAATTLEAIAGDDPTELAARLTYHFSEAGDWV
                     RTARYGWLAGSHAKDVHANVDAGQLLARALDAGKRSRAVRPEEIAKIAEALADVRVAL
                     GEFGEALTAYRTARRRLRGDRVEESRLLYKEARSLASVGSHSEAQRRLGRALSLLESV
                     TSLPAGAQRARASAQRAATYQRQGRPRDQLSWCRRAIDEAEAADARDALATALFVLDA
                     AHMALGEPELATNSGRALTIFDELGDLSSKGGVLNNLGVLAYFAGDWDGALDSYRQAA
                     EAWEQAGDRWSASFATFNTAEILSNQGRYDDAEPLLRDALRVRQAAGTSARAAEVQAE
                     LGRLHARRGDMATALDHLHAARDAYAESGEEGGVLDADARIAEAVLLGGDPERALALA
                     RSGLSRCESFQGGSFMAPGLHRVIGCSYLAAGNLQAGRSALRESLSGAESLGADYEVA
                     TTLDALGRLEQLAGGDGSAARRRDRLFARLGIVSAPAPVISA"
     gene            complement(3266..4738)
                     /locus_tag="AC150V_02425"
     CDS             complement(3266..4738)
                     /locus_tag="AC150V_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761808.1"
                     /translation="MNGYRSFFRCHARGARGACALGLMALLATLSALVLSGAGGAAST
                     PKNYTANVTPSSVTGGTTVTFEVTFENLAATQQLGSANVSIPPAWGGTIAGVSTLPSG
                     SSFDATTVFLRNLGIGAGASATFAVTVDVPCIDASGDWSVVAKQSNNFNGPPGTSFNL
                     VEPSDLTTDVECGVDLVFDGQPTDTARDSGDSSKSAPVEGATVRAIPLGCVTACPTVD
                     LDAANALTIDVIAGSSSPVGVGTAPSGTYDLVDGVATISPGPVISASSGTPVGFYRLQ
                     VSSALDPGADPDDVSDEFVVADAICDHDGTDTDTCELSTEVPSFGNKNVGVGVSAQNL
                     NNDPTNQGSTSLLVSTVGAAPPAGVCPGIDPAGPGTQLDIRPLATRTVVTRFIPKSVR
                     QFTANNGLALMNVCLGSNLEFITKYGPLSERVGDRWYGILPDFPAFTDVGGSFVASPS
                     VLARRSVKGGAEIVFELPYVGLAALTTNGLAAYDPSWWMP"
     gene            complement(4830..5603)
                     /locus_tag="AC150V_02430"
     CDS             complement(4830..5603)
                     /locus_tag="AC150V_02430"
                     /inference="COORDINATES: protein motif:HMM:NF021214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR3761809.1"
                     /translation="MDPYAWSWNVEAMIVVPALTALYVWVTRSRYPTPVWRQACFIGA
                     MVLLLAITISPFETIALEFLLWIHLLQNVILAEWAPLLVVLGLSPALAADLSRPALVR
                     ALTHPFVALPLWIGTYVLWHVPLIYDAALRHPHSILHLEHVTYFVTGVLMWWSVFQDA
                     PHRTSSGAKAVYVFAGFLLSAPIGLTLALIPEPVYDFYVGVPLRLWGLSPLEDQQLGG
                     ITMGVEQAIVFFAIFAYWFFRFLAEQEQLEEGDLLENHP"
     gene            5624..5696
                     /locus_tag="AC150V_02435"
     tRNA            5624..5696
                     /locus_tag="AC150V_02435"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5657..5659,aa:Gly,seq:tcc)
     gene            complement(5698..6405)
                     /locus_tag="AC150V_02440"
     CDS             complement(5698..6405)
                     /locus_tag="AC150V_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761810.1"
                     /translation="MSMQDEDAAGGLAAAVFRDVRQRMPFVPAFLKALAVVDDAALEQ
                     AWLQARALFDDSRTTASADRLAAAAAADLGYAPSPSVQSAVGPFAAELPTLLLVITSL
                     TLTLDGRIEVRAAPTADFPESGPLPDAVPEQRGDHPLFDDICRVYGTTYVPSMYRGLA
                     AAGVLEEPWRAVGPYLDSTPGRDHVMRLALAAEAEAELYPEVAVLGVEAARPVLEQFR
                     RALPRNLVFAVAASVES"
     gene            complement(6425..>7095)
                     /locus_tag="AC150V_02445"
     CDS             complement(6425..>7095)
                     /locus_tag="AC150V_02445"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013553696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0071554 - cell wall organization or
                     biogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR3761811.1"
                     /translation="RFESGHPDMRRAVAVVTTVAWLLVVSSAIAAAPFQSSVKPIPAP
                     LAKRMTGVSWREGCPVHLRDLRLVVARHWGFDGKVRTGRLVVHRDVAREIRSVLERLY
                     EARFPIRRMWLIDAYGGSDFRSIEADNTSAFNCRFVEGTTRWSEHSYGRAIDVNPIEN
                     PYVSGGRTSHRASVSFLDRSRLRPGMAVEGGVLVRAFDAAGWGWGGRWAGVRDYQHFS
                     ASGR"
BASE COUNT         1170 a         2444 c         2478 g         1003 t
ORIGIN      
        1 aaggcgatgc gatccttctg ggtgccgagc cggatttggc gccgatcaca ccgccgttct
       61 gggatcccgc aggcatcgag cttgcgcacc tgctgccggc cgagtacacg ctcgagctgc
      121 gcgtagagcc ggctgacccc gagcaccgcc acgtggcagt cgcattcgtc gagatcggcg
      181 gaaccgactc gcttctggca cgcgacggtg caggagcgct cgccgaggcg ctcgacgagc
      241 gcgtcagcgg cgtccaggag gcgtgcctgc ggttcggcgt gacctttgcg cagacggacg
      301 tctccgcagg cggcacgaag gcgatcctgc tcgcgggagc gccgcgaagc gccggcggcg
      361 acgaggagga gctcctgctc cgtgcagtca gagcgatcgt ggagacgccg gcggaacttc
      421 ctgtccgtgc cggaatcaac tcgggccgcg tcttcgccgg agtcgtgggc ccggcgaccc
      481 ggcgtacgta caccttctac ggcgatgcga tcaacaccgc ggctcggatc atggcccgcg
      541 ccgagcccgg ccagctgctg gcacgcgacg aggtgctcga gcgagctcgc acgacctacg
      601 acatcacgcc gatcgagccc ttcagcgcga agggcaaagc agagcccgtg cacgcgtcgg
      661 cggtcgggcc ggcgaccggc gagcggcagc tggaggcgac cgggccgctc gtcggccgcg
      721 gcaacgagct ggccaccctc ctcgaggcgc tcgacggcgc ccgcgccgga acgggcggcc
      781 tcgtgatcct tcgcggcgag gcgggcctcg gcaagacgcg gctcctcctc gagctgcgcg
      841 agcgggccga gggcgctcgg atcgtcgccg tccactgcga gcaggtcgag cagtcgcgtc
      901 cgtacgcgac ggcgggagcg atcctgcggc gcgcgcttca cgtcgacctc cattcgccgg
      961 cgcgcgaggt cgaagcgcgc ctgcgcttcg aagtcggggg ccgggcgccg gagctcgagc
     1021 cgtggctgcc cctgctgggc atcccgctcg ggctcgacct gccccagacg cccgagacct
     1081 cgcgcctcga cgagaagttc gtgcccgagc ggatagcgga aatggtcgac gccatcctgc
     1141 agacgctcgt tcccgacgcc gctctggtcg tcatcgacga cgcgcactgg ctcgacgagg
     1201 cctcgaacga gctgatcggc cgcctcgcgc gagggctcgc ggagcggccg tggctgatcg
     1261 ccgcagcacg ccgcaacctg gcaggcggcc tcagaacgcc ggacagcatc gagtcgaccg
     1321 ctgtcgacct cgagccgctg tcttcggacg aggcgaaacg cctcgtcgtc gagctgaccg
     1381 cggacgcccc ggtcggcgac cacgtcgcgg acgccgtggc gcgccgttca gcgggcagcc
     1441 cgttgttcgt caccgagctc gtgacggcag tgcgggccgg cgcggacgtg ggccatctgc
     1501 ccgagtcggt cgaggcgctg atggcgatgc agatcgacga gctgccggcc ggcgaccgcg
     1561 ccgccttgcg gcaggcgtcg gtgctcggtg ctcgcttcga gctcgagacg ctcctggcga
     1621 cgctcgagct cgagcccgcg gaaggcctgg cactcctcca acgtctgcaa gcgttcctct
     1681 tcctcgacga cgacggcctc gtccgcttcc gtcacgggct cgtgcgggag tcggcctacg
     1741 aggggctccc ataccgccgt cgacggcagc tgcaccgccg ggcagccacg acactcgagg
     1801 ccattgcagg tgacgatccg accgagctcg cagcccgctt gacctaccac ttctccgaag
     1861 ccggcgactg ggtcaggact gcgcgctacg gctggctcgc cggcagtcac gcgaaggacg
     1921 tccacgcgaa cgtcgatgcc ggccagctac tcgcgcgggc gctcgacgcg ggcaagcggt
     1981 cgcgggccgt gcgcccggag gagatcgcga agatcgcgga ggcgcttgcg gacgtgcgag
     2041 tggcgctcgg agagttcggt gaggcactta ccgcgtaccg aaccgcgcgc cgccgcctcc
     2101 gcggggatcg cgtggaggag tcgaggctcc tctacaagga ggcccgcagc ctggcgagcg
     2161 tgggaagcca ctccgaggct cagcgccgcc tcggtcgggc actctcgctg ctcgagagcg
     2221 tcacgagcct gccggcgggt gcccagcggg cgcgggccag cgcgcagcgc gccgcgacct
     2281 accagcgcca gggccggccg cgcgatcaac tcagctggtg tcgaagggcg atagacgagg
     2341 ccgaggccgc cgatgcgcgc gacgcgttgg cgacggcact cttcgtcctc gacgctgcgc
     2401 acatggctct cggcgagccc gaactggcta cgaacagtgg ccgtgcgctc accatcttcg
     2461 acgagctcgg cgacctgagc agcaaggggg gcgtcctgaa caacctcggc gtgctcgcct
     2521 acttcgccgg agactgggac ggcgccctcg actcctaccg gcaggcagcg gaggcctggg
     2581 agcaggccgg agatcggtgg agtgcctcgt ttgccacgtt caacaccgcg gagatcctct
     2641 ccaaccaggg tcgctacgac gacgcagagc cgctgctgcg ggacgcgttg cgcgtcaggc
     2701 aggctgcggg aacatcagcg cgcgccgcag aggtgcaggc ggagctcggc cggcttcatg
     2761 cccgccgcgg ggacatggcg acggcgctcg accacctgca cgcggcccgc gacgcatatg
     2821 cggagtcggg cgaagagggc ggcgtgctgg acgccgatgc gcggattgca gaggccgtct
     2881 tgcttggcgg agatccggag cgtgccctcg cgctggcccg cagcgggttg agccggtgcg
     2941 agtcgttcca gggcggctcg ttcatggccc ctgggttgca tcgcgtgatc ggctgctcgt
     3001 atctcgccgc ggggaatctt caggccggga gaagcgctct tcgcgagagc ctctcgggtg
     3061 ccgagtcact cggcgcggac tacgaagtcg cgaccaccct cgacgctctc ggcagactcg
     3121 agcagctcgc cgggggagac ggcagtgcag cccgtcgccg tgaccggctg ttcgcgcgcc
     3181 tggggatcgt ctcggccccg gctccggtga tctcggcctg aggccatggg ggccgcggcg
     3241 cggcccccat ctgactcgag cagctctacg gcatccacca gctcgggtcg tatgccgcca
     3301 ggccgttcgt cgtaagcgcc gcgagtccca catagggcag ctcgaagacg atctcggcgc
     3361 cgcccttcac actccgtcgg gcgagcaccg agggtgaagc aacgaatgat ccgccgacat
     3421 cggtgaaagc tgggaagtcc ggcaagatcc cgtaccagcg gtcgccgacg cgctctgaca
     3481 gcgggccgta cttggtaatg aactcgagat tagagccgag acacacattc atcagcgcca
     3541 gcccgttgtt cgccgtgaac tggcggacgc tcttgggaat gaaccgcgtg acgaccgttc
     3601 tcgtcgcgag aggccgaata tcgagctgag tcccagggcc tgccgggtcg atccccggac
     3661 agacaccggc aggcggagct gcgccgacgg tcgaaacgag cagcgacgtc gacccctggt
     3721 tggtcgggtc gttgttgagg ttctgggcgc tgacaccgac gccgacgttc ttgttgccga
     3781 aactcggcac ctcggtcgag agctcacagg tgtcggtgtc cgttccgtcg tgatcgcaga
     3841 tagcgtcggc gacgacgaac tcgtcgctca catcgtccgg atcggcgcct gggtcgagcg
     3901 cggacgagac ttggaggcga tagaagccca ccggcgtccc gctcgaggcg ctgatcaccg
     3961 gcccggggct gatcgtggcg acgccgtcga ccagatcgta cgtaccgctc ggggcagtgc
     4021 ccacgccgac cggtgagctc gagccggcga tgacatcgat cgtcagcgcg ttcgctgcgt
     4081 ctaggtctac ggtcggacag gcggtcacac agcccagcgg gatggccctg accgtcgcgc
     4141 cttccaccgg cgcgctcttg ctcgaatcgc ctgagtcccg cgccgtgtcc gtcggctgcc
     4201 cgtcgaacac caagtcgacg ccgcactcta cgtcagttgt caaatccgac ggctccacga
     4261 gattgaacga ggtgccggga ggtccgttga agttgttcga ctgcttcgcg acgacgctcc
     4321 agtccccgga agcgtcgatg cacggaacgt cgacggtcac cgcgaaggtg gcgcttgctc
     4381 ccgcgccgat gcccaagttc cgcaagaaca cggttgtcgc gtcgaaggac gaaccgctcg
     4441 gcaacgtgct gacgccggct atcgtgcccc cccaggcggg cggaatggaa acgttggcag
     4501 aacccagttg ctgcgtggcg gcgaggttct cgaacgtgac ttcgaaggtc accgtcgtgc
     4561 cgccggtgac ggacgacggg gtgacgttgg ccgtgtaatt cttcggtgtc gacgccgcgc
     4621 caccggctcc actcagaacg agcgccgaca gcgtcgccag caatgccatg agaccgagcg
     4681 cgcaggcgcc acgcgcaccc cgcgcatggc agcggaaaaa gcttcggtac ccattcatct
     4741 tcccctcgct cctctcgttg caagaaactc gacaggccgc gccatgcgac ggccgattcg
     4801 cgcgattcta tggaagcacc tgcgacgtgt cacgggtggt tttccagaag atcaccttct
     4861 tccagctgct cctgctcggc caggaagcgg aagaaccagt aggcgaagat cgcgaagaag
     4921 acgatcgcct gctcgacgcc catcgtgatc ccgccgagct gctgatcctc gagcggcgag
     4981 agcccccaga gccgcagcgg cacgccgacg tagaagtcgt agacgggctc cgggatcagc
     5041 gcgagcgtga gcccgatcgg ggccgagagc agaaagccgg cgaagacgta gacggccttg
     5101 gcgccgctgc tcgtccggtg aggcgcgtcc tggaagacgg accaccacat caggacgccg
     5161 gtcacgaagt acgtgacgtg ctcgaggtgc aggatcgagt gcgggtggcg cagcgccgcg
     5221 tcgtagatca gcggtacgtg ccacaggacg tacgtgccga tccagagcgg gagggcgacg
     5281 aagggatgcg ttagcgcgcg gacgagagca ggacgcgaga ggtcggccgc gagggccggg
     5341 ctgagcccga gcaccaccag tagcggcgcc cattcggcga ggatgacgtt ctgcagcagg
     5401 tggatccaga gcaggaactc gagggcgatc gtctcgaagg gcgagatcgt gattgccagg
     5461 agcaacacca tcgcgccgat gaagcaggcc tgccgccaca ccggtgtcgg gtagcgactt
     5521 cgcgtgaccc agacgtagag cgccgtcagc gcgggaacga cgatcatcgc ctcgacgttc
     5581 cacgaccacg cgtacggatc cacggcgcta cgctagcccc tgcgcgggcg tagctcagtt
     5641 ggtagagcgc cagccttcca agctggatgt cgccggttcg agcccggtcg cccgctctca
     5701 gctctccacc gaggctgcga ccgcgaagac aagattgcgc ggcagcgcgc gccggaactg
     5761 ctcgagcacc ggtcgtgccg cctccacccc cagcacggcg acctccggat agagctccgc
     5821 ctcggcctcc gccgccagtg ccagtcgcat gacgtgatcg cgcccgggcg tcgagtcgag
     5881 gtaggggccg acggcgcgcc agggctcctc caggacgccg gccgccgcaa gcccgcggta
     5941 catgctcggc acgtacgtcg tcccgtagac gcggcagatg tcgtcgaaca gcgggtggtc
     6001 accgcgctgc tcgggaacgg cgtccggcaa cggcccagac tccgggaagt ccgcagtcgg
     6061 cgccgcccgc acctcgatcc gcccgtcgag cgtcaacgtc agcgacgtga tcacgagcag
     6121 gagggtcggc agctcggcag cgaacggccc aacggccgat tgcaccgagg gagagggcgc
     6181 atacccgaga tcggcggcgg cagccgccgc gagacgatcg gccgacgcag tcgtgcggga
     6241 gtcgtcgaaa agggcgcgcg cctgcagcca ggcctgctcg agagcggcgt cgtcgacgac
     6301 ggcaagcgcc ttcaggaatg caggcacgaa cggcatccgc tgcctgacat cgcgaaagac
     6361 agccgcggcg agcccgcctg cagcatcttc gtcctgcatc gacatgcggc cggaggacgg
     6421 catctcaacg accgctggcg gagaagtgct ggtagtccct gacgcccgcc caccgcccgc
     6481 cccagcccca cccggccgcg tcgaacgccc gcacgaggac gccgccctcg acggccattc
     6541 ccgggcgaag ccgcgaccgg tcgagaaacg agacgctggc gcgatgagac gtcctgcccc
     6601 cgctcacgta cgggttctcg atcgggttga cgtcgatcgc gcgtccgtac gagtgctccg
     6661 accagcgcgt cgtgccttcg acgaagcggc agttgaaggc cgaggtgttg tcggcctcga
     6721 tcgagcggaa gtcgctgccc ccgtaggcgt cgatcagcca catccggcgg atcgggaacc
     6781 gtgcctcgta aagacgctcc agaaccgacc ggatctcccg cgcgacatcc cgatggacga
     6841 cgagccgacc cgtcctcact ttcccgtcga acccccagtg gcgcgcgaca acgagcctca
     6901 ggtcgcgcag gtgcacgggg cagccctcac gccaggagac accggtcatc cgcttcgcga
     6961 gcggtgcggg gatcggcttg acggagctct ggaagggagc agccgcgatc gcggacgaga
     7021 cgaccagaag ccacgcgacg gtcgtgacga cggcgacggc tctcctcata tcggggtggc
     7081 cggattcgaa ccgac
//
LOCUS       DBNGNK010000091         9177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_300620,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000091 DBNGNK010000000
VERSION     DBNGNK010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9177
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_300620"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="AC150V_02450"
     CDS             <1..745
                     /locus_tag="AC150V_02450"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3761812.1"
                     /translation="RPHATLAVTKEEATVILAIDTSRSMAADDVEPSRLGAAQVAAAA
                     FLEEVPSKYQVGIVSFSTKADVVLPPTNDRDTALLARQELRLGSGTAIGDAIVASLGA
                     IPRGEEGEEPAPASVLLLSDGKQDAEGGIEPLDAAQQAQELAVPVSTVALGTSEAVVE
                     VPLPGGEVQQQTVAPDPETLGQIAQATGGQFFAAPDDAELEKVYRELGSRLASQDEET
                     EMTYAFAAGGAVLLLVGGLLSTLWFRRPL"
     gene            745..1488
                     /locus_tag="AC150V_02455"
     CDS             745..1488
                     /locus_tag="AC150V_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761813.1"
                     /translation="MRARLACVVIVLAVTMLALVAPSAHAAEECRGLQVCLPVPGAWV
                     VIPPPAKGQSTSTAVWELRCPLRGYIVAGTDALVSDPSIDVSFRAETGSPVSPGVTTS
                     EKVVFTAVYTGTARRLTTFQPHLGCVPTSGGGGRVPTSYTQQPPPAAAGPQEKPLERR
                     VARSRLAAGQTRNAAARCRANERLVGFSHAYGFHSELAPGRSILGKASAKRVVRGSRV
                     IVSGTANATLPGDLEVEIQAHALCAKGGK"
     gene            1485..2486
                     /locus_tag="AC150V_02460"
     CDS             1485..2486
                     /locus_tag="AC150V_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3761814.1"
                     /translation="MSFEAPVWLLTLLVVPLALAGYLALEKRRARYAVSFTNVGVLAE
                     VVGRRTAWRRYLPLVLLLAAVAALCIAVARPTVSLAAPEDRATVILVVDVSGSMRAED
                     VEPTRLDAARDAIRSFLGKSPDGLRVGLIAFSDEPSVIVPPTRDRDLLNQGVELLTPG
                     FGTAIGDAVIRAVELGERVQQGDGEAAAAEPPPSDTAPAEPLTAILLLSDGSQTRGFV
                     QPLESADEAKAKNMPVYTIALGTDEGTIEIFRFGELQVIPVPPDRQTLAQIAETTGGQ
                     YFDAPDAEELKSVYEKLGSEVGRISEPRQATVAFVLAGAGLLLAAAVFSGIWLPRLP"
     gene            2543..3343
                     /locus_tag="AC150V_02465"
     CDS             2543..3343
                     /locus_tag="AC150V_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018751319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761815.1"
                     /translation="MALLSVRDVTLRFGGIVALDAVSFDVQPGQICGLIGPNGAGKTT
                     AFNVITRLYKPDEGTLELEGESLLGTPPHRITSRGIARTFQNLNLFRTMSVLENVLVG
                     GHARGRDVGEPEARATLEYVGLLERANELAAALPYGTQKRVELARALVARPKLLLLDE
                     PAGGLNHEEVEELGAFIQRLREDYGLTVLLVEHHMNLVMSISDHVSVLSFGRVISEGT
                     PAEVQADEAVIEAYLGTEDGVDDQPPRQVGEPAGEAGDVRELGQSDST"
     gene            3340..4047
                     /locus_tag="AC150V_02470"
     CDS             3340..4047
                     /locus_tag="AC150V_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761816.1"
                     /translation="MTALLELRDVEARYGPVVALRGVSLEVGEGQVVAVLGANGAGKT
                     TTLRAVSGLVKRSGDLVFGGKRLPGGPEAVAKAGIAHVPEGRGTFTELTVLENLKLGA
                     YTRRDREVKRDIDRVAEYFPWIGERGGQPAGTLSGGEQQMLSLGRALMSRPRLLMLDE
                     PSLGLAPIVVREIFRIVRELNEKDGLAVLVVEQDARVALDVASSAYVLEVGRVAVAGP
                     SDELRDDESVRRSYLGY"
     gene            4061..4945
                     /locus_tag="AC150V_02475"
     CDS             4061..4945
                     /locus_tag="AC150V_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3761817.1"
                     /translation="MTEFFQQVVAGLASGGIYALLALALVLIHRATGVINFAQGEMAT
                     FSTFVLWWLTAQHGLPYWAGFFLVLLISFGGGIAIHEAIIRPVERGSVLRVVIVTIGL
                     LLLLNGLMTWIWGGEIRAVESPFPTKTIDIGGVTISVQDLGTIAVALATVFVLWLFFQ
                     FTKVGLALRAVAVNPNEARLVGVRVSWMLGLGWGLAALLGAIAGMLTAPSVFLDPNMM
                     QPVLIYAFAAAVLGGIDSPIGAVVGGLTLGVVLNLLGTYVDFVGSDLQLPAALLVILI
                     VLLVKPSGLFGRPQVQRV"
     gene            4942..5955
                     /locus_tag="AC150V_02480"
     CDS             4942..5955
                     /locus_tag="AC150V_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR3761818.1"
                     /translation="MRRSTLLAIAGFVALAVVVYVVPGFVSDFRAQQFAFVGIYTIAL
                     LGLNVLTGFTGQISLGHGAFMAIGGYTTALLIAGNEHFGGGMKDLWTIPIAGLVAGIA
                     GFLFGLPALRLSGLYLALATFAIAVATPAVIKKFESFTGGGSGINLFGLDELTASIEP
                     VTIFGQELNFNNWLYYLCWTIALVAYVGAWLLLRGRTGRAFRAVRDSETAAMSAGVSL
                     ARYKTLAFAVSAAYCGVAGALFAIATTFVNPDTFPIALSIFLLVGVVVGGLGSLVGVI
                     AGAIVIQFLPIWAQEVSKSPGAPAVVYGAFLIVVMFLLPGGFAGLLRRIRGLSGRLSH
                     RSE"
     gene            5952..6944
                     /locus_tag="AC150V_02485"
     CDS             5952..6944
                     /locus_tag="AC150V_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3761819.1"
                     /translation="MTRSGARFAALSAVLALTALVAASSGRADPNATEPGVRSAVIVL
                     GATVPLSGPTAANAAVARGAEAYFAHVNRRGGVHGRRILFRLLDDGGDPARSLPATRR
                     LVTVERVFAVVNAYGTESSLATRGYLNAVRVPQLFVASGAATFGADMTRFPASLGFQP
                     SYPTEGRVLGEYLAGSRPNAKVAVLVQDDDQGRELLGGFRKGAAAGTGVEIVAVQRYD
                     ADEADIPARMAELKASRADTFAVFAAPTFATQAFAAANELGWRPAVLASSDAAARSRP
                     RFSKTLRIRPGGEILPSGSTARSWPSMLQGGTRETFVTSKAWRSRTRSSSCCER"
     gene            6989..7135
                     /locus_tag="AC150V_02490"
     CDS             6989..7135
                     /locus_tag="AC150V_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761820.1"
                     /translation="MRMANNPFLLPGIAIRTGREDQFPVEQVQLRRWENGRWRVFGAL
                     WGRR"
     gene            7232..>9177
                     /gene="cofG"
                     /locus_tag="AC150V_02495"
     CDS             7232..>9177
                     /gene="cofG"
                     /locus_tag="AC150V_02495"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HIR3761821.1"
                     /translation="MRDLHSELLTASLDELMAEARRLRRGRLVTYSPKVFVPLTTLCR
                     DVCGYCTFARPPRRGERAYMSEDEVLDVARKGAAAGCTEALFTLGDQPERRYKVARAE
                     LSMLGCSTTLEYLERCARLVLEETGLLPHLNPGVLTRTELSALRPASASMGIMLESTA
                     ERLAERGGPHWASPDKLPARRLATIRAAGELSIPFTSGILVGIGETRSERIDALLELK
                     ALGAEFGHVGEVIVQNFRAKPGTRMADEPDASFDEQLWSVAVARIVLGPDWHVQAPPN
                     LAFDEFPLLLDAGIDDWGGVSPVTVDHVNPEAPWPEIERLEQATQSRGLELAPRLPLY
                     PEYVSELERWVDPAVRPHVWRAADGMGLAREDRWAPGEPGATPRLVTRDALPLTVSEG
                     GLGEDEIVRLLEARGRERERVLAAADALRRDVCGEDVTYVVTRNIQYTNVCYFRCGFC
                     AFSKGKLAENLRGPAYLGPLEEIVRRSDEAWERGATEVCLQGGIHPGFTGDYYAEVVA
                     AIAEAVPGLHVHAFSALEIWQGAATLAVDLASYLARLRALGLGSLPGTAAEILDDQVR
                     AVICPDKVTTEQWLDVHAAAHRAGLRSNVTMMFGHVDGSRNWARHLIRARDQQAATGG
                     FTEFVPLPFVPMEAPIYLTGRARRG"
BASE COUNT         1257 a         3165 c         3230 g         1525 t
ORIGIN      
        1 gcgacctcat gcgactctcg ccgtgacgaa ggaagaggcg accgtcatac tcgcgatcga
       61 cacgtcgcgg tcgatggcgg ccgacgacgt cgagccgagc cgcctcgggg ccgcccaggt
      121 cgccgccgca gcctttctcg aggaggtgcc cagcaagtat caggtcggca tcgtctcgtt
      181 ctcgaccaag gcggatgtcg tcctcccgcc gacgaacgac cgcgacacgg cgctgctcgc
      241 gcgtcaggag ctcagactgg gctcgggtac ggcgatcggc gacgcgatcg tggcctctct
      301 gggagcgatt ccgcgcggcg aggagggcga ggagccggcg cccgcttccg ttctgcttct
      361 gtccgacggc aagcaggacg ccgagggcgg catcgagccg ctcgacgctg cgcagcaggc
      421 ccaggagctc gccgtgccgg tctccaccgt cgccctcggc acctccgagg ccgtcgtcga
      481 ggtgccgctt ccgggcggtg aggttcagca gcagacggtc gcgcccgacc cggagacgct
      541 cggccagatc gctcaggcga ccggcggcca gttcttcgcc gccccggacg acgccgagct
      601 cgagaaggtc taccgggagc tgggctcgcg gctggcaagc caggacgagg agaccgagat
      661 gacgtatgcg ttcgcggccg gaggggcagt gctcctgctc gtcggcggcc tgctctcgac
      721 gctctggttc cggaggccgc tctgatgcgg gctcgactgg cttgcgtcgt catcgtgctc
      781 gccgtgacga tgctggcact cgtggctccc tctgcgcatg cggccgagga gtgccgcggc
      841 ctccaggtct gtctgcccgt gcccggggcc tgggtcgtga tcccgccgcc ggcgaagggg
      901 cagtcgacgt cgacagccgt ttgggagctc cgctgccccc tgcgcggcta catcgtcgcg
      961 ggcaccgacg cgctcgtgag cgatccctcg atcgacgtca gcttccgcgc cgagaccggc
     1021 agccccgtct cgcccggcgt gacgacgagc gagaaggtcg tcttcacggc cgtctacaca
     1081 gggacggcaa ggaggctcac gacgttccag ccgcatctcg gctgcgttcc tacgagcggg
     1141 ggaggcgggc gcgtgccgac ctcgtacacg cagcagccac cgccggccgc cgccgggccg
     1201 caggagaagc cgctcgagcg acgcgtcgcg cgctcccggc tcgcggccgg gcaaacgcgt
     1261 aacgccgccg ctcggtgtcg cgcgaacgag cggctcgtcg ggttctcgca cgcatacgga
     1321 ttccattcgg agctcgcgcc gggccggtcg attctcggca aggcaagcgc gaagcgtgtc
     1381 gtgcgcggga gccgagtgat cgtgagcggg acggcaaatg cgacgctgcc cggcgatctc
     1441 gaggtcgaga tccaggcgca cgcgctctgc gcgaagggcg gcaagtgagc ttcgaggccc
     1501 ccgtctggct gctcacgctg ctcgtcgtgc cgctcgcgct ggccggctac ctcgcgctgg
     1561 agaagcggcg ggccagatac gccgtcagct tcacgaacgt tggcgtcctg gccgaggtcg
     1621 tcggacgtcg gaccgcctgg cgtcgctacc tgccgctcgt gctcctgctc gcggccgtcg
     1681 ccgcgctgtg catcgccgtc gcccgtccga ccgtctcgct ggcggcgccg gaggacaggg
     1741 caacggtgat cctcgtcgtc gacgtgtcgg gctcgatgcg ggccgaggac gtggagccga
     1801 ctcgtctcga cgcagcacgg gacgcgattc ggagttttct cggcaagtcg ccggacggcc
     1861 tgcgggtcgg tctcatcgcc ttctcagacg agccgtcggt gatcgtcccg ccgactcgag
     1921 atcgcgacct gctgaaccag ggcgtggagc tcctcacgcc cggcttcggg accgccatcg
     1981 gcgatgccgt gatccgtgcc gtcgagctcg gcgaacgcgt gcagcagggg gacggcgaag
     2041 cggccgcggc cgagccacct ccgagcgata ccgctcccgc ggaaccgctg accgcgatcc
     2101 tcctgctctc ggacgggtcg cagacgcggg gcttcgtcca gccactcgaa agcgccgacg
     2161 aggcgaaagc caagaacatg cccgtctaca cgatcgcgct cggcaccgac gaggggacga
     2221 tcgagatctt ccgcttcggc gagctccagg tcatcccggt gccgcccgac cgtcagacgc
     2281 ttgcccagat cgccgagacc accgggggcc agtacttcga cgcccccgac gccgaggagc
     2341 tcaagtcggt ctacgagaag ctcggctccg aggtcgggcg gatcagcgaa ccgcgccagg
     2401 ccaccgtcgc gttcgtcctt gcgggcgccg ggctgctgct cgcggcggcc gtcttctcgg
     2461 gcatctggct gccacgcctg ccctagccct gccctcgtct ttggtcattc ggcggcggcc
     2521 ggtttagtgt aggcgcgctc cgatggcgct cctctccgtc cgcgacgtca ccctgcgttt
     2581 cggcggcatc gtcgctctcg acgcggtgtc gttcgacgtg cagcccggtc agatctgcgg
     2641 cctgatcggc ccgaacggcg ccggcaagac gaccgccttc aacgtgatca cgcgcctgta
     2701 caagccggac gagggcacgt tggagctgga gggggagtcg ctgctcggta cgccgccgca
     2761 caggatcacg tcgcgcggca tcgcccgcac cttccagaac ctcaacctct tccgcacgat
     2821 gtccgtgctc gagaacgtcc tcgtcggagg acacgcgcgc ggccgcgacg tcggcgagcc
     2881 ggaggcgcgg gcaacgctcg agtacgtcgg tctgctcgag cgggccaacg agctggcggc
     2941 agcactcccc tacggcacgc agaaacgcgt cgagttggcg cgggcgctgg tggcgcgacc
     3001 caagctgctc ctgctggacg agcccgcagg cggtcttaac cacgaggagg tcgaggagct
     3061 cggcgcgttc atccagcgcc tccgcgagga ctacggcctc accgttctcc tcgtggagca
     3121 tcacatgaac ctggtgatga gcatctccga tcacgtgagc gtgctcagct tcgggagggt
     3181 gatctcggag gggacacctg ccgaggtgca ggcggacgaa gcagtgatcg aggcctatct
     3241 cggcaccgaa gacggggtcg acgaccagcc accccggcag gtgggggagc cggccggcga
     3301 ggcgggcgac gttcgagagc tcgggcagag tgacagcacg tgacggccct gctcgagttg
     3361 cgggacgtcg aggcacgcta cgggccggtg gtggctttac gcggcgtgtc gctcgaagtg
     3421 ggggaggggc aggtcgtcgc cgtgttgggg gccaacggtg cgggcaagac gacgaccctg
     3481 cgcgcggtgt cggggctcgt gaagcggagc ggcgatctcg tcttcggcgg caagcgcctt
     3541 cccggcggcc cggaggcggt cgccaaggcg ggcatcgcac atgtgccgga ggggcgcggc
     3601 acgttcaccg agctgacggt gctggagaac ttgaaactcg gcgcctacac ccgccgcgac
     3661 cgggaggtga agcgggacat cgaccgcgtc gcggagtact tcccctggat cggggagcgc
     3721 ggcgggcagc ccgcggggac gctgtcgggg ggcgagcagc agatgctctc gctcggacgt
     3781 gcgctcatga gccggccgag actcttgatg ctggacgagc cgtcgctcgg cctggcgccg
     3841 atcgtcgtgc gagagatctt ccgcatcgtc cgtgagctga acgagaagga cggtctggct
     3901 gtcctggtgg tcgagcagga cgcgcgtgtc gccctcgacg tcgcgagttc cgcctacgtg
     3961 ctcgaagtcg gccgggtcgc cgtcgcgggg ccgagcgacg agttgcgcga cgacgagtcg
     4021 gtccgacgga gctacctggg gtactgaggc gaacgagacg gtgaccgagt tcttccagca
     4081 ggtcgttgcg gggctcgcct ccgggggcat ctatgcgctg ctcgcgctgg ctctcgtcct
     4141 cattcaccgg gccactgggg tgatcaactt cgcgcagggc gagatggcga ccttctcgac
     4201 tttcgtcctc tggtggctca cggcccagca cggtcttcct tactgggccg ggttcttcct
     4261 cgtgctgctc atctcgttcg gcggcggcat cgcgatccac gaggcgatca tccgcccggt
     4321 cgagcgcggc tccgttctgc gcgtggtgat cgtgacgatc gggttgctcc tgctgctgaa
     4381 cggactcatg acgtggatct ggggcggcga gatccgagcg gtcgagagtc cgttcccgac
     4441 gaagacgatc gacatcggcg gcgtcacgat ctcggtgcag gacctcggga cgatcgccgt
     4501 cgcactggcg acggtcttcg tgctgtggct cttcttccag ttcacgaagg tcggtctcgc
     4561 gctccgcgcg gtggccgtca acccgaacga ggcccggctc gtcggcgtcc gcgtgtcctg
     4621 gatgctcggg ctcggctggg ggctcgccgc gttgctcggg gcgatcgccg ggatgctcac
     4681 ggcgccgagc gtcttcctcg acccgaacat gatgcagcct gttctcatct acgccttcgc
     4741 ggcggccgtg ctcggcggga tcgactcgcc gatcggggcg gtcgtgggtg gcttgacgct
     4801 cggagtcgtg ctcaacctgc tgggcacata cgtcgacttc gtcggctccg acttgcagtt
     4861 gccggcggcg ctcctcgtga tcctcatcgt cctcctcgtc aagccgtccg ggttgttcgg
     4921 gcgtccgcag gtgcaacgcg tatgaggcgc tcgacgctgc tcgcgatcgc cggcttcgta
     4981 gctctcgccg tggtcgtcta cgtcgtgccg gggttcgtca gcgacttccg ggcgcagcag
     5041 ttcgccttcg tcggcatcta caccatcgcg ctgctcggcc tcaacgtcct caccggcttc
     5101 acgggtcaga tctcgctcgg tcacggcgcc ttcatggcga tcggcggcta cacgaccgcg
     5161 cttctgatcg cggggaacga gcacttcggc gggggaatga aggatctttg gacgattccc
     5221 atagccgggc tcgttgcggg tatcgccggc ttcctgttcg gcctgccggc gctgaggttg
     5281 tcgggcctct acctcgcgct cgcgacgttc gccatcgccg tcgcgacgcc cgccgtgatc
     5341 aagaagttcg agagcttcac cggcggcggc tcgggcatca acctgttcgg cctcgacgag
     5401 ctcacggcct cgatcgagcc ggtcacgatc ttcggccagg agctcaactt caacaactgg
     5461 ctctactacc tctgctggac gattgccctg gtcgcgtacg tgggcgcctg gctgctcctg
     5521 cgcggccgga cgggacgcgc cttcagggcg gttcgtgata gcgagaccgc ggcgatgtcg
     5581 gccggcgtga gcctcgcccg ttacaagacg ctcgcattcg cggttagcgc tgcctactgc
     5641 ggagtcgccg gcgccctgtt tgcgattgcg acgacgttcg tcaacccgga cacgttcccg
     5701 attgccctct cgatcttcct cctcgtcggc gtcgtcgtcg gtgggctcgg ctcgctcgtc
     5761 ggcgtgatcg ccggtgcgat cgtcatccag ttcctcccga tctgggcgca agaggtgtcg
     5821 aagtcccccg gggcgccggc cgtcgtctac ggagcgtttc tgatcgtcgt gatgttcctg
     5881 ctgcccggcg gcttcgccgg gctcttgcgc cggattcgtg gcctgtccgg gcgactgtcc
     5941 catcgctccg aatgacccgc tcgggagccc ggttcgctgc gctgtcggcc gtgcttgctc
     6001 ttacggcact ggttgcagcc tcgtccggac gcgccgatcc gaatgccacc gaaccgggcg
     6061 tccggtcagc cgtgatcgtg ctcggcgcga cggttcccct ctcgggcccg accgcggcga
     6121 acgccgccgt tgcccgcggc gcggaagcgt acttcgccca tgtcaaccgc cggggcggtg
     6181 tgcacgggcg gaggatcctc ttccggctgc tggacgatgg cggtgacccg gctcgctcgc
     6241 tgcccgccac ccggcggctc gtcaccgtcg agcgcgtctt cgccgtcgtc aacgcctacg
     6301 gcacggagtc cagcctcgcc acgcgtggct acctgaacgc ggtgcgcgtt ccgcagctgt
     6361 tcgtcgcttc cggcgccgcg acgttcggcg ccgacatgac gcgtttcccc gccagcctgg
     6421 gcttccagcc ctcctatccg accgaaggac gcgtgctcgg tgagtatctc gccggctcgc
     6481 ggccgaacgc caaggtggcc gtgctcgtgc aggacgacga ccagggcagg gagctgctcg
     6541 gaggcttcag gaagggcgct gcggccggga ccggcgtaga gatcgtggcg gttcagcgct
     6601 acgacgccga cgaggccgac atccccgccc gcatggccga gctgaaggcc tcgcgcgccg
     6661 acaccttcgc cgtctttgca gcacccactt tcgccaccca ggccttcgcg gcggcaaacg
     6721 agctcggctg gcgcccggcg gttctggcga gctcggacgc cgcggcgcgg tctcggccac
     6781 gtttctcaaa gaccctgcgg atccggcctg gcggagagat cctgccctcc ggctctaccg
     6841 ctcgatcatg gccgagcatg ctccagggcg gaactcggga gaccttcgtc acgtccaagg
     6901 catggcggtc gcgcacacgg tcgtcgagct gttgcgaaag gtagggcgga atccaacgcg
     6961 ggacgcgctc ctcctcgcgg cgcggacgat gaggatggcg aacaacccct tcctgcttcc
     7021 cggtatcgcg atcaggacgg ggcgcgagga tcagttcccg gtggagcagg tgcagctgcg
     7081 tcgctgggag aacggtcgct ggcgcgtctt cggcgcgctc tgggggcgcc gctaggcacg
     7141 ggggtcgcgc gccgcccggt ctgacgcgcc ggttatagtc tcggctccgg cgcgaggaga
     7201 ccgcccggcg gctgtcacaa ccgagtcacc gatgcgcgat ctccactccg agcttctcac
     7261 ggcgtcactg gacgagctga tggccgaggc caggcgcctc cgccgcggcc ggctcgtcac
     7321 gtactcgccg aaggtcttcg tacccctgac cacgctctgc cgcgacgtct gcggctactg
     7381 cacgtttgcc cggccgccgc gccgcggtga gcgtgcgtac atgtccgagg atgaggttct
     7441 cgacgtcgcc cggaagggcg ccgcggccgg ctgcacggaa gcgctcttca cgctgggtga
     7501 ccagccggaa cgccgctaca aggtcgctcg tgccgagctc tccatgcttg gttgctccac
     7561 gacgctcgag tacctggagc gctgcgcccg cctcgtactg gaggagacag ggttgctgcc
     7621 gcacctcaat ccgggggtcc tgacgaggac ggagctgtcg gcgcttcgac ctgcctcggc
     7681 ctcgatgggg atcatgctcg agtcgacggc cgagcgccta gccgaaagag gaggccccca
     7741 ctgggcatcg ccggacaaac tgccggcgcg gcggctcgcg acgatccggg cagcgggcga
     7801 gctgtcgatc ccgttcacga gcggaatcct ggtcgggatc ggcgagaccc gttcggagcg
     7861 catcgacgcg ctgctggagc tgaaggcgct gggcgccgag ttcggtcacg tcggcgaggt
     7921 gatcgtgcag aacttcaggg ccaagcccgg cacgcgcatg gcggacgagc ccgacgcgtc
     7981 gttcgacgag cagctgtggt cggtcgcggt cgcccgcatc gtcctcgggc cggactggca
     8041 cgtgcaggcc ccgccgaacc tcgccttcga cgagttcccg ctgctgctcg acgccgggat
     8101 cgacgactgg ggcggcgtct cgcccgtcac ggtcgatcac gtcaatcccg aggcgccgtg
     8161 gcccgagatc gagcgcctgg agcaggccac gcagtctcgc ggcctcgagc tggcgccgcg
     8221 gctcccgctc tatccggagt acgtctccga gctcgaacgc tgggtcgacc cggccgtccg
     8281 gcctcatgtc tggcgggcgg cggacgggat ggggctggca cgcgaagacc ggtgggcacc
     8341 cggggagccc ggggcgaccc cccgtctcgt cacgcgcgac gccctcccgc tcacggtttc
     8401 ggaaggcggg ctgggcgaag acgagatcgt gcgactgctc gaggcacgcg ggcgggaacg
     8461 tgagcgcgtg ctcgccgccg ccgacgccct gcgccgcgat gtctgcggcg aagacgtcac
     8521 ctacgtcgtc acgagaaaca tccagtacac gaacgtgtgc tacttccggt gcggcttctg
     8581 cgccttctcg aaagggaagc tggccgagaa cctccgcggg cccgcgtatc tcggtccgct
     8641 cgaggaaatc gtccgccgca gcgacgaggc gtgggagcgc ggcgcgacgg aagtctgcct
     8701 gcagggcggc attcatccgg gcttcaccgg cgactactac gcggaggttg tcgctgcgat
     8761 cgcagaggcc gtgccgggct tgcatgtcca cgcgttttcc gcgctcgaga tctggcaggg
     8821 agccgcaacg ctcgctgtcg atctcgcttc ctacctcgcg cggctgcgcg cgctggggct
     8881 cggctcgctc cccgggacgg cggcagagat cctcgacgac caggtccgcg cggtcatctg
     8941 cccggacaag gtcacgaccg agcagtggct cgacgttcac gccgcggcgc atcgggccgg
     9001 gctccggtcg aacgtgacga tgatgttcgg gcacgtcgac ggctctcgga actgggcccg
     9061 gcatctgatc cgagcgcgcg accagcaggc cgcgacgggc ggcttcaccg agttcgtccc
     9121 gcttcctttc gtacccatgg aagcgccgat ctatctcacg ggacgggctc gtcgagg
//
LOCUS       DBNGNK010000092         4218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_659560,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000092 DBNGNK010000000
VERSION     DBNGNK010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_659560"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="AC150V_02500"
     CDS             <1..570
                     /locus_tag="AC150V_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3761822.1"
                     /translation="SIARYRTGGKHLSFWFLSQRVLPPIAVIIPIFLLYSRYSEDWFG
                     YALIDTRTGLVLLYTVFALPFTVWMMYTYFRQMPPSLEEAALVDGCTRIQALRKIAWP
                     LAKPGVVSAAAFAFIFSWTEFLFAVVLARDHAVTLPVALAGMVTSFQGNLYGEASALT
                     MVSLIPALLLGILLQRHLVRGLTLGAVQG"
     gene            567..1637
                     /locus_tag="AC150V_02505"
     CDS             567..1637
                     /locus_tag="AC150V_02505"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3761823.1"
                     /translation="MSQGSYQQGMARPDVDIGAGEIQLKPVSERTTLVTGGGGFIGGH
                     LVRALLDTGRKVAVFDVQDFIPEARFVVGSDVEQVPFERGSIADQARVLDAFRVHRPD
                     EVVHMAMILDPAVLAMNRSTAFDVNVGGVLNVLEAMIAFGTARLVNFSSIGVLPRVLY
                     KPVDGAHPVLLPDTGPGTGFYGSTKVAAEAMCFAYNQVLGVDFRTIRPSAVYGLGMNQ
                     YVGPIKAMVESAVRGESAHFEFGGAHPRPYTHARDIAGLVLAVLDAPEDADRIFYGST
                     GGDMVTTSEVAQIVRELVPGADVEIAEELSEAEKPVVALRGELNVDNAREQLGWSPQY
                     GDIRDGIAQYIEHYREYIGAAS"
     gene            1639..2496
                     /locus_tag="AC150V_02510"
     CDS             1639..2496
                     /locus_tag="AC150V_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3761824.1"
                     /translation="MAPRANVRLGLSDVARIAVIVPYTVFLVLPLYWVASSSVKTPTE
                     VVSATWYPRDPSLEYFRNALGDLRGWQGVQNSLVVAVATTVLAVIIGTAAAYSMARFR
                     TGGKHLSFWFLSQRVLPPIAVIIPIFLLYSRYSEEWFGYALIDTHVGLVLLYTVFALP
                     FTVWMMYTYFRQMPPSLEEAALVDGCTRIQALRKIAWPLAKPGVIAAAAFAFIFSWTE
                     FLFALVLTRAEVVTLPVVIGGLAASTQGSGYGSVAALGVISLGPALVLGLFLQRHIVR
                     GLTLGAVEG"
     gene            2688..3797
                     /locus_tag="AC150V_02515"
     CDS             2688..3797
                     /locus_tag="AC150V_02515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018783761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR3761825.1"
                     /translation="MTFDVAVLPGDGIGPEVVAEAVKVLEAAERLWGGEVSFRFESFD
                     AGAGAWQRTGSAIADDVYAACEQAHAIVLGAAGLPDARHPDGREAGGDVIFRLRFGLD
                     LYAGIRPVKLYRGVPSPLGDPGAGIDYVILRENVEGVYAGRAGGSRVEREVVADTSII
                     TRAGTQRIAERAFELARRRNGRPLDGEAAVTCVDKSNVLASYAFFRSIVEETAASYPD
                     VSYEGIYVDAAALYLVQRPDAFDVIVTENMFGDILSDLGAATVGGLGLAPSADVGARH
                     GVFQAAHGSAPDIAGRGVANPQATILSTAMMLDWLALLHENDAAATVGREIEAAVRET
                     LSDTATRTPDLGGAATTEAVGDAVVAALERAAAAA"
     gene            3794..>4218
                     /locus_tag="AC150V_02520"
     CDS             3794..>4218
                     /locus_tag="AC150V_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3761826.1"
                     /translation="MTEDELRQLIDDPAARRSVSAVELAEACLRRIAAENPRLRALIT
                     VTDDLALADAALADRARASGEPSPLDGMPIVVKDAIDMAGLRTTMGSRQFENHVAEAD
                     APVVGKLRAAGAVILGKANLHEIAFGGTSRNEAFGAVVNP"
BASE COUNT          606 a         1471 c         1433 g          708 t
ORIGIN      
        1 tcgatcgcgc gctaccgcac cggcggcaag cacctctcct tctggttcct gtcgcagcgg
       61 gtgctgccgc cgatcgccgt gatcatcccg atcttcctcc tctacagccg ctactcggag
      121 gactggttcg gctacgccct gatcgacacg cgaaccggcc tcgtgctcct gtacacggtg
      181 ttcgcgctgc cgttcaccgt ctggatgatg tacacgtact tccgacagat gccgccctcg
      241 ctggaggagg cggcactggt ggacggctgc acgcggatcc aagccttgcg caagatcgcc
      301 tggccgttgg cgaaaccggg cgtcgtctcg gcggcggcgt tcgcgttcat cttctcctgg
      361 accgagttct tgttcgcggt cgtcctcgcg cgcgatcatg cggtcacgct gcccgtcgcc
      421 ctggccggca tggtcacgtc cttccagggc aacctgtacg gcgaggcaag cgccttgacg
      481 atggtgtcgc tgatacccgc gctcctgctc gggatcctcc tgcagcggca cctcgtgcga
      541 ggcctgacac taggagcggt gcaaggatga gccagggttc ctaccaacag gggatggccc
      601 gcccggacgt cgacatcggc gccggcgaga tccagctcaa gccggtctcg gagcggacga
      661 cgctcgtcac gggaggcggt gggttcatcg gcggacacct cgttcgcgcg ctgctcgaca
      721 ccggccgcaa ggtcgccgtc ttcgacgtgc aggacttcat tccggaggca cgcttcgtcg
      781 tcggcagcga cgtcgagcag gtccccttcg agcgcggctc gatcgccgac caggcgcgcg
      841 tgctcgacgc cttccgcgtg caccggccgg acgaggtcgt gcacatggcg atgatcctcg
      901 atcccgccgt cctcgccatg aaccgctcga cggcgttcga cgtcaacgtc ggcggcgttc
      961 tcaacgtgct cgaagcgatg atcgccttcg gcacggcgcg cctcgtcaac ttctcctcga
     1021 tcggagtcct tccgcgcgtg ctctacaagc cggtcgacgg agcccatccg gtgttgctcc
     1081 cggacaccgg ccccggcacc ggcttctacg gctcgaccaa ggtcgcggcc gaggcgatgt
     1141 gcttcgccta caaccaggtg ctgggggtcg acttccgcac gattcgcccg tcggcggtgt
     1201 acgggctcgg gatgaaccag tacgtcggcc cgatcaaggc gatggtcgaa agcgcggtgc
     1261 gcggcgagtc ggctcacttc gagttcggtg gcgcgcatcc gcgcccgtac acgcatgccc
     1321 gcgacatcgc cgggctcgtc ctcgcggtgc tcgacgcacc ggaagacgca gaccggatct
     1381 tctacggctc gacgggcggc gacatggtga cgacgagcga ggtcgcgcaa atcgttcgcg
     1441 agctcgtgcc aggtgccgac gtcgagatcg ccgaagagct gagcgaggcg gagaagccgg
     1501 ttgtcgcgtt gcgcggtgag ctgaacgtcg acaacgcgcg cgagcagctc ggctggagcc
     1561 ctcagtacgg cgacatccgc gacggcatcg cccagtacat cgagcactac cgagagtaca
     1621 tcggcgccgc gagctgagat ggcgccgcgc gccaacgtcc ggctgggact gtcggacgtc
     1681 gcgaggatcg cggtgatcgt cccctacacg gtgttcctcg tcctgccgct gtactgggtc
     1741 gccagctcct ccgtcaagac gccgacagaa gtggtcagtg ccacctggta cccacgggac
     1801 ccgagcctcg agtactttcg caacgcgctg ggcgacctgc gcggctggca gggggtgcag
     1861 aacagcctcg tcgtcgccgt tgccacgacg gtgctcgcgg tcatcatcgg gacggcggcc
     1921 gcctactcga tggcccggtt ccgcaccggc ggcaagcatc tctccttctg gttcctgtcg
     1981 cagcgggtgc tgccgccgat cgcggtgatc atcccgatct tcctcctcta cagccgctac
     2041 tcggaggaat ggttcggcta cgcgctgatc gacacgcacg tggggctcgt gctcctgtac
     2101 acggtgttcg cgctgccgtt caccgtctgg atgatgtaca cgtacttccg gcagatgccg
     2161 ccctcgctgg aggaggcggc actggtggac gggtgcacgc ggatccaggc cttgcgcaag
     2221 atcgcctggc cgttggcgaa accgggcgtg atcgcggcgg cagcgttcgc gttcatcttc
     2281 tcctggaccg agtttctctt cgcgctggtg ctgacgcgag ccgaggtcgt cacgctcccc
     2341 gtcgtgatcg gtggcctggc cgcttcaacg cagggctcgg gatacgggtc cgttgccgcg
     2401 ctcggggtca tctcactcgg gcctgcactc gtcctcggtc tgttcttgca gcgccacatc
     2461 gtgcgcggcc tgaccctcgg cgccgtcgag ggctgagttc gggctcacgc ttgcgtcgct
     2521 gaccgggacg gcgcggtgcc ggtgccgctg cgacgcgcgg ccgggccatg gttgtagtgt
     2581 caccgtgtgc tcgtcgctag agctctgtca acccgaacct cggccgccgc cgagctcggc
     2641 atgagccagg ccgtagaccg ccaacgcacc tctctcgccg cctgctcgtg accttcgacg
     2701 tcgctgtcct tccaggcgac gggatcgggc ccgaggtcgt cgctgaggcg gtcaaggtgc
     2761 tcgaggccgc cgagcggctc tggggcggcg aagtctcctt ccgcttcgag tccttcgacg
     2821 caggtgcggg cgcctggcag cgaaccggtt cggcgatcgc ggacgacgtc tatgcggcgt
     2881 gcgagcaggc acacgcgatc gtgctgggcg cggccggtct cccggacgcc cgtcaccctg
     2941 acgggcgcga agccgggggc gacgtcatct tccgcctccg cttcggcctc gacctctatg
     3001 cgggcatccg cccggtcaag ctctatcgag gcgtcccgtc gccgctcggc gatcccggcg
     3061 cgggcatcga ctacgtgatc ctgcgcgaga acgtcgaggg cgtgtacgcg ggccgggccg
     3121 gcgggagccg cgtggagcgt gaggtcgtcg ccgacacgag catcatcacg cgcgccggca
     3181 cccagcggat cgctgaacgc gccttcgagc tggcgcggcg acgcaacgga cgcccgctcg
     3241 acggagaggc ggcagtcacc tgcgtcgaca agtcgaacgt gctcgcgtcg tacgcgttct
     3301 tccgctcgat cgtcgaggag accgccgcct cgtatcccga cgtctcgtac gagggcatct
     3361 acgtggacgc tgcggcgctc tacctcgtgc agcgaccgga tgcgttcgac gtgatcgtga
     3421 ccgagaacat gttcggcgac atcctgtccg atctcggcgc cgccacggtc ggcggcctgg
     3481 ggctcgcgcc gtccgccgac gtcggtgctc gccacggcgt cttccaggcg gcgcacggct
     3541 cggcgcccga catcgcgggc cgaggcgtcg ctaatcctca ggcgacgatc ctgtcgactg
     3601 cgatgatgct cgactggctc gcactgctcc acgagaacga cgccgcggca acggtcggcc
     3661 gcgagatcga ggccgccgta cgggaaaccc tctcggacac ggcgacccgc acgcccgatc
     3721 tggggggagc cgccacgacc gaggccgtcg gggacgccgt cgtcgccgcg ctcgagcgag
     3781 ctgctgcggc cgcgtgaccg aggacgagct cagacagctg atcgacgacc cggcggcgcg
     3841 gcgaagcgtc tcggcggtcg agctcgccga ggcctgcctc cgccggatcg ccgccgagaa
     3901 cccgcgactc cgcgcgctga tcacggtcac ggacgatctc gcgctcgccg acgccgctct
     3961 ggccgaccga gcccgcgcga gcggagagcc gtcgccgctc gacggaatgc cgatcgtggt
     4021 caaggacgcg atcgacatgg ccggtctgcg gacgacgatg ggatcacgac agttcgagaa
     4081 ccacgttgcg gaagctgacg cgccggtcgt aggcaagctg cgcgcggccg gagccgtgat
     4141 cctcggcaag gccaacctgc acgagatcgc gttcggcgga accagccgca acgaggcatt
     4201 cggagcggtt gtcaaccc
//
LOCUS       DBNGNK010000093         4429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1949321,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000093 DBNGNK010000000
VERSION     DBNGNK010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1949321"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2534)
                     /locus_tag="AC150V_02525"
     CDS             complement(<1..2534)
                     /locus_tag="AC150V_02525"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761827.1"
                     /translation="MNELFGIPVDTLLVMLLVALAVALAGLAVFAFRDPILLKLAVRS
                     ASRRPGRTALIVVGLMLGTTIIAAALTTGDTMNHTIRSTAVKALGSTDETIAAKGAVG
                     DIPGALGSATGIGWFDEATVARIDAALEPSGLADGVTGAIVEQVAVQAPKQRQSEPSV
                     VLFAADPARMDGFSPIRGSVGNRLSLDDLRPGEVYLNRKAATELRIEPGDTVNVYTGG
                     APISARVRDAVRFDGAGTADAALLMPLAEAQRLLGHEGEVKGVLVSNQGDATSGARHS
                     DEVAALLGPIVDPLGLEVETVKQDAIRDAEEAGNAFMAFFTTFGSFSIAAGILLIFLI
                     FVMLAAERRGELGIARAIGTRRGHLVEMFTFEGVAYDFAAAVVGVLLGALVALGMVFV
                     MASAFGAGDADEGFQIEFAVSTRSLLIAFALGVLLTLVVVAVSAWRVSVMTISTAIRN
                     LPEPPGTRRRRRLVLALAALALGILMAVSGAASGAATPLMLGISLALISLVPLFRLAG
                     VPERLAFTGCGLAVVVVLMLPWSIWEAVFGELAMNFSTWIVSGLMIVVGTVWVIVYNA
                     DVLLALVMRLAGRLHGLAPVLRMSMAYPLRARFRTGTTLVMFTLVVFTLVTGTASTSS
                     FTEAFSGPEEFGGGFQVRAGTSGGAPIEDMATALRRARGVRAGEFTAVASQSVLAVEA
                     TQVDTGRPAESYPVRGLDRPFLTHTTFGLGAIARGYQSADEVWNALSTREGLAVVDSF
                     VVPRRDQFGFGIAPDFKLSGFYFEDGTFEPIPVDVVDAQTGKRLELTVIGVLKDTAPF
                     EMVGISTSQDTLSAAFPGRASPTIHYFATAPEVDPEDAAARLESAFL"
     gene            complement(2538..2849)
                     /locus_tag="AC150V_02530"
     CDS             complement(2538..2849)
                     /locus_tag="AC150V_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761828.1"
                     /translation="MYSRVTLLEIDTMRVDVTDAVALFREEVLPGVQEQEGYEGVIVL
                     TTPEGKGMIISVWATEEAASAAAGFASETLERYMTMFKAPPGREHYEVAVYDLSSVSM
                     A"
     gene            complement(2824..3540)
                     /locus_tag="AC150V_02535"
     CDS             complement(2824..3540)
                     /locus_tag="AC150V_02535"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761829.1"
                     /translation="MAPIVEARAVWKTYSGAVDVAALRGVDLVLEQGEVVAIMGPSGC
                     GKTTLLNCLSGLDSLDGGEVLIEGTALAAMSDRERTDYRARRMGFVFQFYNLIPVLTA
                     VENVELPLLVSRVAGGEARKRALEALDLVGLVERANHVPDALSGGERQRVTIARSLVN
                     DPAIVWADEPTGDLDSENAAEIIELMRRLNLERGLTFLIVTHDIAVGRKTDRIVRMLD
                     GQVVEEQRLEVAHVLAGHAA"
     gene            4008..>4429
                     /locus_tag="AC150V_02540"
     CDS             4008..>4429
                     /locus_tag="AC150V_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR3761830.1"
                     /translation="MTVACPACGAGHEPGARFCSSCGAALWRDCPGCGSEQPAAAAFC
                     GSCGIALSPGARRAEGGDTREERRIVTVLFADLAGSTSLGEQLDPEDVRTLQGDLYEL
                     VSREIDAHGGICEKFVGDAVLAVFGVPQTHEDDAERAVR"
BASE COUNT          783 a         1507 c         1455 g          684 t
ORIGIN      
        1 aggaaggccg actcgagcct tgccgccgcg tcctcgggat cgacttcagg ggctgtcgcg
       61 aagtagtgga tggttgggct ggcgcgaccc gggaacgcgg cggacagggt gtcttgggag
      121 gtcgagatgc cgaccatctc gaacggggcg gtgtccttga ggacgccgat tactgtcagt
      181 tcaaggcgct ttcctgtttg tgcatcgacg acgtcgaccg ggatcggttc aaacgtgccg
      241 tcctcgaagt agaagccgct gagcttgaaa tcgggggcga tcccgaagcc gaactggtcg
      301 cgcctgggga cgacgaagct gtcaacgacg gcgagccctt cgcgggtgct gagcgcgttc
      361 cagacctcgt cggcagattg gtagccgcgg gcgatcgctc cgagcccgaa tgtcgtgtgt
      421 gtgaggaagg ggcgatcgag gccgcgaacc gggtagctct cggcggggcg tccggtgtcg
      481 acctgagtcg cttcgacggc cagcaccgac tggctcgcga ccgcggtgaa ttcgccggcg
      541 cggacacccc gtgctcggcg aagcgccgtc gccatgtcct cgatcggcgc gccaccggag
      601 gtgccggccc gcacctggaa gccgcctccg aactcttctg ggccgctgaa tgcctccgtg
      661 aacgaactgg ttgaggcggt gccggttacg agcgtgaaga cgacgagtgt gaacatcacc
      721 agcgttgtgc cggtgcggaa gcgcgccctg agcgggtagg ccatagacat ccgtaagacc
      781 ggagcgaggc cgtgcaggcg gccggcgagt cgcatgacga gcgcgagcaa cacgtcggcg
      841 ttgtagacga tcacccagac cgtgccgacg acgatcatca ggccggagac gatccaggtc
      901 gagaagttca tcgcgagctc gccgaacacc gcctcccaga tgctccaggg cagcatgagc
      961 acgacgacga cggccagccc gcagccagtg aacgccaagc gctcggggac acctgccagg
     1021 cgaaacagcg ggacgaggct gataagcgcc agcgagatgc ccaacatgag cggcgtcgca
     1081 gcgccggagg cggcgccgga gacggccatg aggatcccga gcgcgagtgc cgcgagcgcc
     1141 agcacgagcc gtcgccgccg tcgcgtcccg ggcggctccg gcaggttgcg gatcgccgtc
     1201 gagatcgtca tcacgctcac acgccaggcc gagaccgcga cgacgacgag cgtcagtagg
     1261 acgccgagcg cgaaggcgat cagcaaactc ctggtggaga cggcgaactc gatctggaag
     1321 ccctcgtcgg cgtcgcccgc accgaatgcg gaggccatca cgaacaccat gccgagtgcg
     1381 acgagcgcgc ccagcagcac tcccacgacg gcggccgcga agtcgtaggc gacaccttcg
     1441 aaggtgaaca tctccacgag gtgtccgcgg cgagtgccga tcgcgcgggc gatcccgagc
     1501 tcaccgcggc gttcggcggc gagcatcacg aagatcagga agatcaggag gatcccggct
     1561 gcgatcgaga acgacccgaa cgtcgtaaag aaggccatga aggcattgcc ggcctcctcg
     1621 gcatccctga ttgcgtcctg cttgaccgtc tccacctcca ggcccagcgg atcgacaatc
     1681 ggaccgagca gcgctgcgac ctcgtccgag tgacgtgccc ccgacgtcgc gtcaccctga
     1741 ttggagacca gtacaccctt gacctctccc tcgtggccca gtagtcgctg cgcttcggcg
     1801 agcggcatga gaagcgccgc gtctgctgtg cccgcgccgt cgaagcggac ggcatcgcgc
     1861 acgcgggccg aaatcggcgc gccgccggtg tacacgttca cggtgtcgcc gggctcgatc
     1921 cgcagctcgg tggctgcctt ccggttcagg tacacctcgc cgggtcgtag gtcgtccagc
     1981 gaaagtctgt tcccgacgct gccccggatc ggtgagaagc cgtccattcg tgccgggtcg
     2041 gcggcgaaga ggacgacgct cggctcgctc tgccgctgtt tcggtgcttg cacggcgacc
     2101 tgctcgacga tcgccccggt cacgccgtcc gcgagccccg agggctccag tgctgcgtcg
     2161 atccgagcga cggtcgcctc gtcgaaccag ccgatgcccg tcgcggaccc gagtgcgccg
     2221 ggtatgtcgc cgacggcgcc tttcgcggcg atcgtctcgt ctgtgctgcc aagggccttg
     2281 actgccgtgg agcgaatcgt gtggttcatc gtgtcaccgg tggtcagcgc cgcagcgatg
     2341 atcgtcgtgc cgagcatcag cccgacaacg atcagtgcgg ttcggcccgg gcggcgagag
     2401 gcgctgcgaa cggcgagctt gagcagaatc ggatcacgga aggcgaagac ggcaagccca
     2461 gccaacgcca ccgccagcgc caccagcaac atgacgagga gcgtgtcgac cgggatgccg
     2521 aacagctcgt tcatcagcta tgccatcgac acgctcgaca ggtcgtacac cgctacctcg
     2581 tagtgctcac gacccggcgg cgccttgaac atcgtcatgt agcgctcgag cgtttcgctg
     2641 gcgaaaccgg cagccgcact cgcggcttcc tctgtcgccc agaccgagat gatcatgccc
     2701 ttgccttctg gggtcgtgag cacgatcacc ccctcgtagc cctcttgctc ctgcacccct
     2761 gggagtacct cctcgcgaaa gagagcgacg gcatcagtga cgtcgacgcg catggtgtcg
     2821 atctcaagca gcgtgacccg cgagtacatg tgccacctcc agtcgttgct cctccactac
     2881 ctggccgtcg agcatgcgca cgatgcgatc ggtctttcga ccgaccgcga tgtcatgggt
     2941 gacgatcagg aacgtcagcc cgcgctcaag gttgagccgg cgcatcagct cgatgatctc
     3001 ggccgcgttc tccgagtcga ggtcgccggt cggctcgtcc gcccacacga tcgccgggtc
     3061 attgacgagc gagcgggcga tcgtcacgcg ctggcgctca cccccggaaa gcgcgtcggg
     3121 cacgtgatta gcgcgctcga cgagaccaac gaggtcgagc gcctcaagcg cccgcttccg
     3181 ggcctcgccg ccggcgacac gggagacgag cagcggcagc tccacgttct cgaccgccgt
     3241 cagtaccggg atcaagttgt agaactggaa cacgaaaccc attcgccgag ctcgatagtc
     3301 cgttcgctcg cggtcagaca tcgccgcgag ggccgtaccc tcgatcagca cctcgccacc
     3361 atcgagcgag tcgagcccgg aaagacaatt cagcagcgtt gtcttgccgc agccactcgg
     3421 gcccatgatc gcaaccacct cgccctgctc gagaacgaga tcgacgcccc gcaaggcagc
     3481 gacgtcaacc gcacccgaat acgtcttcca gactgctcgg gcctccacga tcggcgccat
     3541 cgaccgcctc ctttcttcgt ccaacctcga ggctgacgcc acggcgccgc atcgtcatcc
     3601 gcaggacgcc tgaaacgcgc aggggcgaac taccgactcg aggccaggac tccgacaact
     3661 tcgccttccg aagccggtca cgttctcgga tgaccgattg ctgcaaacgg aactgcaaac
     3721 gaacatcgag cgacgtgctg atccgcctcc gcactcgacg tcactaaacc tccgaaacac
     3781 gtgaattccg gtaccagcct cgactagagg gggctggacg agaccgtcgc caacgcactt
     3841 gaaagccgtt gagccgagag gctccgtgag ttcgaatctc acccccgccg cttccagttc
     3901 aagctgatac ccgcgccgtc ggttcggggc tccgggggct ccgaggacga ccggccggac
     3961 atttctcctg ctcatctcct gctccgcccc acgagtacgc tgcgctcgtg acggtcgcct
     4021 gtccagcgtg cggtgccggg cacgagcccg gtgctcggtt ctgcagcagc tgcggcgcgg
     4081 cgctctggcg ggactgcccc ggctgcggca gcgagcagcc ggcggcggcc gcgttctgcg
     4141 gctcgtgcgg catcgcgctc tcacccgggg ctcgccgcgc cgagggaggt gacacgcgcg
     4201 aggagcggcg catcgtgacc gtcctcttcg ccgatctcgc cggctcgacg tccctcggag
     4261 agcagctgga tccggaggac gtcagaactc tgcagggcga tctctacgag ctcgtcagcc
     4321 gggagatcga tgcgcacggc ggcatctgcg agaagttcgt cggagacgcg gtgctcgccg
     4381 tcttcggagt cccgcagacg cacgaggacg acgccgagcg ggcggtccg
//
LOCUS       DBNGNK010000094         4294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_230613,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000094 DBNGNK010000000
VERSION     DBNGNK010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_230613"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="AC150V_02545"
     CDS             <1..993
                     /locus_tag="AC150V_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014135.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR3761831.1"
                     /translation="ALMFLLAVTPGVMSAFLDNLTAILLVVPITLVIADLLGINPLPL
                     VLLQVIASNIGGTATLIGDPPNILIGSHVDELGFVDFIVNLAPVSFFTLAIVTGILAL
                     FYRRSLVVTPEHANEIERLDPARDLRDGKKVKRTLAVLVGTIVMFFLHQPLHIEPAVV
                     ALGGATVMLFVAADDVEKALERVEWATIFFVIGLFIMVGGLDERGIIDEVATWLADVT
                     EGSRVAEGMAILWGSAGGSALVDNIPFTTAMLPVVDRIQGEEFDDGLWWSLSLGACFG
                     GNATMIAAAANVAAAGILDRTGNSISFTRFLMVGLPVTMVSLVIATAYLLAFQL"
     gene            993..1445
                     /locus_tag="AC150V_02550"
     CDS             993..1445
                     /locus_tag="AC150V_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3761832.1"
                     /translation="MDHLGDLQLVGASVPQTATFAAAVAALFAARVPAIAVLDDEEKV
                     TGIFAEGDLIRGVFPGYIGELQHSGFLRDDPSSLDELTRVARAAPVGDYARPIEPLDA
                     DDSQSHAAGRFLHTGEHALPVVDADDRFLGMLSISALCHARLDPPAAS"
     gene            1446..2648
                     /locus_tag="AC150V_02555"
     CDS             1446..2648
                     /locus_tag="AC150V_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007081881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3761833.1"
                     /translation="MPLAASAGVADALFDLLIVLVAAKAGDELFKRLGQPTIVGEILA
                     GVLIGSSVLGWVDLTDVLEVFAELGVVFLLFWVGLETRIGELKAVGRISAGVGVLGVV
                     IPFLAGLGIGLAWGRSVTTSTFIGASLVATSVGITSAVLLDLGKLRTRAGRTILGAAV
                     VDDILAMLIVAVAAGIAASGEGGVNVRELVFVATVAVAFVAFFALGGTSLMRRFPGIL
                     TRPRFAESPLLPAVILCLGLAAIAAEIGLAAIIGAFLAGLMIAETKEQHPIEEEVAPL
                     YAFFTPFFFVSIGIQIDLDALGSWGAIALLSAITAAAVVSKLTGAALGALQLGRAQAL
                     FVGWGMVPRGEVGIVVAGIGILTGVLSEEAFAVIVGMSVLTTLIVPPILRRQSAKLPG
                     EPATDQPS"
     gene            complement(2636..3493)
                     /locus_tag="AC150V_02560"
     CDS             complement(2636..3493)
                     /locus_tag="AC150V_02560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3761834.1"
                     /translation="MAEVRTIGVVGLGAMGAGIAQLAIEAGYETVGREVTDELGGKAA
                     ERIGHFLTRKVEKGQLEQEARDDAVARLKLTTELADFADCDLVIEAIVEDLDAKLELL
                     RDLSAIVTRDAVLATNPSALSVTEIAAAATGPERVVGMHFFNPAPLMPLVEIVRAELT
                     GDDAVETASVVGERMGKTPVHCHDTPGFVVNRILIPLMNDCIRVLDEARVTPEALDAA
                     MKHGAGWPMGPCTLVDLVGIDVHVHASEALYEKLRESRMAPPPRLVAMKNAGLLGRKT
                     GRGFYRYDG"
     gene            complement(3505..3891)
                     /locus_tag="AC150V_02565"
     CDS             complement(3505..3891)
                     /locus_tag="AC150V_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761835.1"
                     /translation="MTDWLLVRGRGDRPLHRSVDPAAITRHASSRRPRVEPGDRAILY
                     AAVWQAIFGVVEIAGEPERDAERERWAWSFPLRPILVVRDLDRAPAVEEAGVFPHSLW
                     RHSHIRLTGEQFEEARRLIARAAERR"
     gene            complement(4012..>4294)
                     /locus_tag="AC150V_02570"
     CDS             complement(4012..>4294)
                     /locus_tag="AC150V_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007317487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761836.1"
                     /translation="GLSWDDIAVIEVNEAFASVVLQFLADTGLEERWRAGAVNPNGGG
                     ISLGHPLGGTGARITATLLNELERREARYGLASMCIGQGQAIAAVIERL"
BASE COUNT          583 a         1524 c         1446 g          741 t
ORIGIN      
        1 gcgctcatgt tcctcctcgc cgtgacaccc ggtgtgatgt cggcgttcct cgacaacctc
       61 accgcgatcc tcctcgtcgt cccgatcacg ctcgtgatcg cagacctgct cggcatcaat
      121 ccactcccgc tggttctgct gcaggtgatc gcgagcaaca tcggcggcac ggcgacgctg
      181 atcggcgatc cgccgaacat cctgatcggc agccacgtgg acgagctcgg cttcgtcgac
      241 ttcatcgtca acctcgcacc ggtctccttc ttcacgctcg cgatcgtgac gggaatcctg
      301 gctttgtttt atcgccgctc gctcgtcgtc acgcccgagc acgcgaacga gatcgagcgg
      361 ctcgatcccg cccgcgacct gcgtgacggc aagaaggtca agcgcacgct cgccgtcctc
      421 gtcggcacga tcgtgatgtt cttcctgcac cagccgttgc acatcgagcc ggccgtggtg
      481 gccctcggcg gcgccacggt gatgctgttc gtcgccgccg acgacgtcga gaaggctctc
      541 gaacgggtcg agtgggcgac gatcttcttc gtcatcggcc tcttcatcat ggtgggaggc
      601 ctcgacgagc gcggcatcat cgacgaggtg gcgacctggc tcgccgatgt caccgagggc
      661 tcgagagtcg cagaggggat ggccatcctc tgggggtcgg ccggcggctc ggcgctcgtc
      721 gacaacatcc cgttcacgac ggcgatgctc ccggtggtcg accgcattca aggtgaggag
      781 ttcgacgacg ggctctggtg gtcgctctcg ctcggtgcct gcttcggtgg caacgcgacc
      841 atgatcgccg ccgcggccaa cgtcgccgcc gccggcatcc tcgaccggac cggcaactcg
      901 atctcgttca cccgcttcct catggtcggc cttccggtaa caatggtctc gctcgtcatc
      961 gcgaccgcgt atcttttggc cttccagctc tgatggatca cctcggcgat ctgcaactcg
     1021 tcggcgcgtc ggtgccgcag acggcgactt tcgccgcagc cgttgccgcc ctcttcgccg
     1081 cccgcgttcc cgcgatcgcc gtgctcgacg acgaggagaa ggtgaccggc atcttcgccg
     1141 agggcgacct cattcgcggc gtcttccccg gctatatcgg cgagcttcag cacagcggct
     1201 ttctccgcga cgacccgtcg agtctcgacg agcttacgcg ggtggcccgg gccgcgccgg
     1261 tcggcgatta cgcacggccg atcgagcccc tcgacgcgga cgacagccag agccacgcgg
     1321 cggggcgctt tctccatacc ggggagcacg cgctgccggt ggtcgatgcc gacgaccggt
     1381 ttctcggcat gctctcgatc tcggccctct gccacgcccg gctcgatccg cccgcagctt
     1441 catagatgcc actggccgcc agcgccggcg tcgccgacgc tctcttcgat ctgctcatcg
     1501 tgctggtcgc cgcaaaggcc ggcgacgagc tcttcaagcg gctcgggcag ccgacgatcg
     1561 ttggcgagat cctggcgggc gtcctgatcg gctcgtcggt gctcggctgg gtcgacctga
     1621 cggacgtgct cgaggtcttc gcggagctgg gcgtggtgtt cctcctcttc tgggtcggtc
     1681 tcgagacgcg gatcggcgag ctcaaggcgg tcggccgcat ctcggcaggc gtcggcgttc
     1741 tcggcgtcgt gatcccgttt ctggcgggcc tcgggatcgg cctcgcctgg ggccgtagcg
     1801 tgacgacgag cacgttcatc ggcgcctcgc tcgtcgcgac gagcgtgggc atcacgagcg
     1861 ccgtcctcct cgacctcggc aagctccgca cccgcgccgg gcggacgatc ctcggcgcgg
     1921 cggtcgtcga cgacatcctc gccatgctca tcgtcgcggt cgcggccggg atcgctgcct
     1981 cgggcgaagg cggcgtcaat gtccgagagc tcgtcttcgt ggccaccgtc gccgtcgcct
     2041 tcgtggcgtt cttcgccctc ggcggaacga gcttgatgcg gcgcttcccc gggatcctca
     2101 cgcggccccg cttcgccgag tcaccgctgc tgccggccgt catcctctgc ctcggcctgg
     2161 ccgcgatcgc agctgagatc ggactggctg cgatcatcgg cgcgttcctc gcgggcctga
     2221 tgattgccga gacgaaggag cagcacccga tcgaggagga ggtagcgccc ctctacgcgt
     2281 tcttcacgcc gttcttcttc gtctcgatcg gcattcagat cgacctcgac gcgctcggct
     2341 cgtggggcgc gatcgcgctg ctgtcggcga tcaccgccgc tgccgtcgtc tccaagctca
     2401 ccggcgcggc gctcggcgct ctgcagctgg gccgggccca ggcgctgttc gtaggctggg
     2461 gaatggtgcc gcgcggcgag gtgggaatcg tcgtggccgg aatcggcatc ctgaccggtg
     2521 tgctgagcga ggaggccttc gcggtcatcg tcgggatgtc cgtgctgacg accttgatcg
     2581 tgcctcccat cctgcgacgc cagagcgcga agcttccggg cgagccggcg acggatcagc
     2641 cgtcgtagcg gtagaagccg cgcccggtct tgcgaccgag gaggccggcg ttcttcatcg
     2701 ccacgagccg cggcggcggc gccatgcgcg actcgcgcag cttctcgtac agagcctctg
     2761 acgcgtgaac gtgcacgtcg atgcccacga ggtcgacgag cgtgcacggc cccatcggcc
     2821 agccggcacc gtgtttcatc gcagcgtcga gcgcctctgg cgtcacgcgt gcctcgtcga
     2881 ggacgcggat gcagtcgttc atcagcggga tgagaatccg gttgacgacg aagccgggcg
     2941 tgtcgtggca gtggacaggt gtcttgccca ttcgctcgcc gacgacggag gccgtctcga
     3001 cggcgtcgtc gccggtgagc tcggcgcgga cgatctcgac cagcggcatc aggggggccg
     3061 gattgaagaa gtgcatcccg acgacccgct cggggcccgt cgccgccgcg gcgatctcgg
     3121 tcaccgagag cgcggacggg ttcgtcgcca gcaccgcgtc gcgcgtgacg atcgcgctca
     3181 ggtcgcgcaa gagctcgagc ttggcgtcga gatcctcgac gatcgcctcg atcacgaggt
     3241 cgcagtcggc gaagtcggcc agctccgtcg tcagcttcag gcgtgccacc gcgtcgtcgc
     3301 gggcctcctg ctccagctgc cctttctcga ccttcctcgt cagaaagtgc ccgatccgct
     3361 cggcggcctt tccgccgagt tcgtccgtca cctcgcggcc gaccgtctcg tagccggcct
     3421 cgatcgccag ctgtgcgatc ccggcgccca tggcgccgag gccgacgaca ccgatcgtgc
     3481 gcacctccgc cacggcggcg gatcctacct tcgctcggcc gcgcgagcga tgaggcgccg
     3541 agcctcctcg aactgctccc ctgtcagccg gatgtgcgag tgacgccaga gcgagtgcgg
     3601 gaagacgccc gcttcctcca ccgcgggcgc acggtcgaga tcgcgaacga cgaggatcgg
     3661 acggagcggg aacgaccagg cccagcgctc gcgctcggcg tctcgctccg gctcgcccgc
     3721 gatctcgacc acgccgaaga tcgcctgcca gaccgcggcg tacaagatcg cgcggtcgcc
     3781 cggctcgacc cgcggccgcc gtgaggatgc gtgccgggtg atcgcagcgg gatcgacgct
     3841 ccggtggagc ggccggtcac cgcgaccgcg gacgagcagc cagtcggtca ccgccgcgga
     3901 cggaaggtga cgccgagggc cgtcgtccgc tggagctgcg cgcgagggaa ggggtattcc
     3961 cgcggtcgga tgctctggcc cacgcagaag acggagaggg cagacgctcg tctagaggcg
     4021 ttcgatgacg gccgcgatcg cctgaccctg gccgatgcac atcgaggcga gcccgtagcg
     4081 cgcctcccgc cgctcgagct cgttcagcaa cgtggccgtg atacgggcgc cggtcccgcc
     4141 gagcgggtgc ccgagtgaga tgccgccgcc gttggggttg acggcgccgg cgcgccagcg
     4201 ctcctcgagc cccgtgtcag cgaggaactg gagcacgacg gaggcgaacg cctcgttcac
     4261 ctcgatcacg gcgatgtcgt cccagctcag gccc
//
LOCUS       DBNGNK010000095         8013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_517299,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000095 DBNGNK010000000
VERSION     DBNGNK010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8013
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_517299"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2856
                     /locus_tag="AC150V_02575"
     CDS             <1..2856
                     /locus_tag="AC150V_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3761837.1"
                     /translation="RLQQVAEPGTVVVGERTQRVTRDAVAYARIPDLSVKGKSRPLPA
                     WRATGVLRSAGERRPGFGAPLIGRDDELALLRLTASKVRRERTPQLLTVFGHAGVGKS
                     RLVAEFVGELGDARVLVGRCVPYGDGITYLPLAEIAQTLAGILEDDPNEVGLAKLRAA
                     VADVLPEEELEEIVRVMAWTIGLSVPDSATGLGGAGDVTVSLQHAWVRLLGALGRETL
                     LVLLLEDIHWASEPLLDLLDQVLASLEDSAVLMICPARPELLGARPSWSAARLNASAL
                     TLAPLGGSDAEALLQALLQAEEIPDAVAQRILEPAEGNPFFVEEMLAMLVEQGALERR
                     EGRWAATAGLEEATLPDSIHGIIAARIDLLAPAEREALRRCSVMGRNFWPSAVGVDDD
                     LVARLVPSALVSEQPASVFSGRREFSFKHALTYEVAYATVPRAERRELHRRVAEWLRD
                     TVPDRQAELPELVAYHYQEALEYGGADDELQGRAFTALLAAGDAALGRGAYASAERLL
                     ARALELASDEDERARGLLMSARAVTHRGGYEDAVARLDEALVLSDRRGSATLRADALG
                     WKARASWLRGDWREALRSAEAAVAALDGAPESAELARALARLSQIQMLRGLPEADETS
                     ARAIDVSRRTGERAAEANARTNLMTARTSRGIPPAPMEMEEVVDLGLAAGAPDEGVRA
                     VVNFLWGAALLGPLDEVEPILHSVVRRLAPGLAAENYARYLDLSLAALVYVPSGRWVE
                     ADAVGAVEEPPAAASNRLVWLWLVTGQALRRNDVALLDRHLPEFSEAALASEEPQRIV
                     PLASVAMPRALLREDRESLRTLAGTVLQLPWMAITHSPSATPIMRALAAAQEPELLEE
                     LARRLDEPESSAALEVGSGVARGLLARLHGRAEEAAQVLADAEAEVRQRGRRYEAACL
                     ALEVGAALELAGDGEGARATRERADAVLAPLGCINPY"
     gene            3001..5289
                     /locus_tag="AC150V_02580"
     CDS             3001..5289
                     /locus_tag="AC150V_02580"
                     /inference="COORDINATES: protein motif:HMM:NF042648.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HIR3761838.1"
                     /translation="MQVVGYADTYSVEPGSAIGFMVSSHSPTFRAEVIRLIHGDANPA
                     GPGFKSEPVESDITGEYPGQVQVIRPGSYVRVPHGPALDPDGGFTLQMWILATTPEKE
                     RQTLVAKASPGGGGYELGLEAGRLTLRLLRDGQFESLAVTRPVAPHTWYFVSAAYDAG
                     AGEGALTLQPLPATASELADTARGSLSTAQSDRAGLGAAPSTDLLIAARSATEGRGLV
                     ESFYNGKIDSPRLYGRALSAQELERLREGCDPKEIPDLVAAWDFSADISSSRVSDVSG
                     NGLHGEAVQRPGRAVTGHNWDGRESNWKHSPEQYGAIHFHDDDLDDAGWERSLQWTVP
                     PGLASGVYALHLEVPGGEDDIPFVVRPKTGQPTAKILFLMPTFSYLAYGNEHLLGSPA
                     TRSMMIDLGVSLDTLDYPSQPQDEYILENRLSSLYDSHSDGSGVFYSTRMRPVVNMRP
                     KYFMQGLDDGSGSPHQFNADLHLVDWLHQHGYEFDVITDEDLYREGLDVLDGYDVVLT
                     GTHHEYWAERMLDAMKAYLDGGGRLMYLAGNGFYWVTELEPEHGHTIEIRRRGPATRT
                     WDAAPGEGHLSFTGELGGLWRYRNRAPQQLVGVGFTAQGTGAGRPYTRQPDSFDPRAA
                     FVFEGVGDDELIGDFPCLVSSYGAAGFELDRVDYGLGTPHRTMLLATATGFSDSFQHV
                     SEEITVTTSKHGGPASRLVRADMVLLEYPNGGAVFSPASITWCGALTYNNYDNNVSRV
                     TRNVLDRFTSPGPAIRAEEKPL"
     gene            complement(5351..6283)
                     /locus_tag="AC150V_02585"
     CDS             complement(5351..6283)
                     /locus_tag="AC150V_02585"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3761839.1"
                     /translation="MIAGARVQCIYNPEVGRERQWGAGSLTRVAQPRRALVIGGGPAG
                     LEFCRVAAARGHEVTLLEREPEAGGHVRVQALLPDRQELGQIAVWLAAQATKNGAEIR
                     TGVEVSDADLDSLLTDERPDHIVVATGAPYRRDGWQGQTAASVPGWESGNCASWDQVA
                     EGKVAVSGSILVIDDLQDIAGPLTAVKLAREGASVRLVTRWPMVAMETMPEVYYLWTR
                     QALLEAGVEILTDLFATRISGREVEFVNVYAPERVTRLEADAIVFNTGRQSDNALYHA
                     FRARGVSVETIGDATAPRGTYEAVYEGHRAARRL"
     gene            complement(6338..7381)
                     /locus_tag="AC150V_02590"
                     /pseudo
     CDS             complement(6338..7381)
                     /locus_tag="AC150V_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            complement(7515..>8013)
                     /locus_tag="AC150V_02595"
     CDS             complement(7515..>8013)
                     /locus_tag="AC150V_02595"
                     /inference="COORDINATES: protein motif:HMM:NF023431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HIR3761840.1"
                     /translation="AWPTRAGSAYRSVGRGERAMAREEDAAREIVLSLDGLLRKRVVF
                     SSESLTDHVTANAVRVRPLDRVGVLAGDLPTSAQRRVLEIIRTYLANYPPAMAREALA
                     RVDRAGIARTRFGWAGSTRPGLPHYYRLQGPTFLLEFDNSRNSGTHIHSVWRDFQRDF
                     GRHLL"
BASE COUNT         1217 a         2806 c         2773 g         1217 t
ORIGIN      
        1 cggctgcagc aggttgcgga gcccgggacg gtggtcgtcg gcgagcgcac gcagcgcgtc
       61 acgagggacg cggtcgccta cgcgcggatc ccggatctca gcgtgaaggg aaagagccgg
      121 ccgttgccgg cgtggcgggc gaccggcgtg ctccggtctg ccggcgagcg ccggcccgga
      181 ttcggcgcac ccctgatcgg ccgcgacgac gagctcgctc tgctccgtct caccgcgagc
      241 aaggtgcggc gtgaacgcac gccccaactc ctgacggtct tcggccacgc cggcgtcggc
      301 aagagccgcc tcgtggcaga gttcgtcggc gagctcgggg acgcccgcgt cctcgtcggg
      361 cgctgcgtgc cctacggcga cggcatcacg tacctcccgc tcgccgagat cgctcagacg
      421 ctggcaggca tcctcgagga cgacccgaac gaggtcggcc tggcgaagct tcgtgcggcg
      481 gtcgcggacg tgctccccga agaggagctg gaggagatcg tccgggtgat ggcatggaca
      541 atcggcctct cggtgcccga cagcgctacc ggcctcggcg gcgcaggtga cgtgaccgtc
      601 tcactccaac atgcctgggt gaggctcctg ggcgcgctgg gtcgagagac gctcctcgtg
      661 ctcctgctgg aggacatcca ctgggcgtcc gagccgctgc tcgacctcct cgatcaggtg
      721 ctcgcatcgc tcgaggacag cgccgtgctg atgatctgtc ccgcccgacc cgagctcctc
      781 ggcgcgaggc cgtcctggag cgctgcgcgc ctcaacgcct cggcgctgac gctcgcgccg
      841 ctcggtggct ccgacgccga ggcgctgttg caggcgctgc tgcaggccga agagatcccg
      901 gacgccgttg cccagcggat cctcgagccg gccgagggca atccgttctt cgtcgaggag
      961 atgctggcga tgctcgtcga gcaaggcgct ctggagcgac gcgaggggcg atgggcagcg
     1021 accgccgggc tcgaggaagc gacactcccc gactcgatcc acggcatcat cgccgcgcgg
     1081 atcgatcttc tcgcgccggc cgaacgcgaa gcactcaggc gttgctccgt aatgggacgc
     1141 aacttctggc cgtccgcggt cggcgtcgac gacgacctcg tagcgcggct cgtcccgagt
     1201 gcgctcgtct ccgaacagcc cgcctccgtg ttctcgggac gccgcgagtt ctccttcaag
     1261 catgcactca cgtacgaggt ggcctacgcg acggtgccgc gggccgagcg ccgggagctg
     1321 cacaggcggg tcgccgagtg gcttcgtgac acggtgcccg accggcaggc cgagctgccg
     1381 gagctggtcg cctatcacta ccaggaggcc ctcgagtacg gcggggcgga cgacgagctc
     1441 cagggccgag cgttcacagc actgctcgcc gccggagacg cagcactggg gcgcggcgcc
     1501 tacgcgtccg cggagcggct gctggcacgc gcgctcgagc tggcatccga cgaggacgag
     1561 cgggcgcggg ggctgctgat gagcgcacgc gccgtcacgc accgaggcgg ctacgaggac
     1621 gcggtcgccc gtctcgacga ggcactggtg ctctcagacc gacgagggag cgcgacgctg
     1681 cgcgccgacg cgctgggttg gaaagcgcgg gcgagctggc tccgtggaga ctggcgcgag
     1741 gcgctccggt cggccgaggc ggccgtcgcg gcgctcgacg gcgcgcccga gtcggccgag
     1801 ctcgcccggg cgctcgcacg actgtcgcag atccagatgc tccgcgggct tccggaggcg
     1861 gacgagacgt ccgcacgcgc gatcgacgtc tcgcgtcgga cgggcgaacg ggcggccgag
     1921 gcgaacgcgc ggacgaacct catgacggct cgtacctcgc gcggcattcc accggcgccg
     1981 atggagatgg aagaggtcgt cgacctgggc ctggccgcgg gggcgcccga cgagggcgtg
     2041 cgggccgttg tcaacttcct ctggggcgcg gcgctcctcg gtcctctcga cgaggtcgag
     2101 ccgattcttc acagcgtcgt gcggcgcctc gcaccgggtc tcgcagcgga gaactatgct
     2161 cgctacctcg atctgtcgct cgccgcgctc gtctacgtgc cctcgggtcg ctgggtcgaa
     2221 gcggacgccg tcggtgccgt cgaggagccg cccgccgcgg ccagcaaccg gctcgtctgg
     2281 ctgtggctgg tcacgggtca ggcgcttcgg cggaacgacg tcgcgctgct agatcgccac
     2341 cttcccgagt tcagcgaggc ggcgctggcc agcgaggagc cgcagcggat cgtgcccctg
     2401 gcgagcgttg cgatgccacg agctctcctc agggaagatc gcgagtcgct gcggacactc
     2461 gccggaaccg tgctgcagct gccctggatg gcgatcaccc attcgccgag cgcgacgccc
     2521 atcatgcgcg cgctcgccgc tgcgcaggag ccggagctgc tcgaagagct cgcgcgccgt
     2581 ctcgacgagc ccgagagctc cgcggccctc gaagtcggct ccggcgttgc ccggggactg
     2641 ctggcgcgac ttcacggtcg cgccgaagaa gctgcacagg tgctggccga cgcggaagcg
     2701 gaggtgcggc aacgtgggag gcgctacgag gccgcctgcc tggcgctcga ggtcggggcc
     2761 gcgctcgagc tcgccggcga cggcgagggc gcgagggcga cacgtgagcg tgccgacgct
     2821 gtgctcgccc cgctcggttg catcaacccg tactgacctc cggccgcgcc ggctcccgcg
     2881 ctctcctgtt ctcttctccc gcatgtgtgc gcgtcggcac ccgtcgggaa ttacgctcct
     2941 tctgacgact gagcctgatg agccgcgggc gcaagtccgc tgtggagagg agacccggac
     3001 atgcaggtgg ttggctacgc cgacacgtac agcgtcgagc cgggatccgc catcgggttc
     3061 atggtcagct cgcactcgcc gacgttccgc gcggaggtga tccggctcat ccacggggat
     3121 gcgaatcctg cgggcccggg cttcaagagc gagccggtgg agagcgacat cacgggcgag
     3181 tatccggggc aggtgcaggt catacggccg ggctcctacg ttcgcgtgcc acacggccct
     3241 gcgctcgatc ccgacggcgg cttcactctc cagatgtgga tcctggcgac tacaccggag
     3301 aaggagcgcc agaccctggt cgccaaagcg agccccggcg gcggtgggta cgagctcggg
     3361 ctcgaagcgg gtcgccttac cctgcgcctg ctacgcgacg gtcagttcga atctctcgcc
     3421 gtcacgagac ctgtggcacc ccacacgtgg tacttcgtct ccgccgccta cgacgcgggc
     3481 gccggcgagg gcgccctgac actgcagccc ctgccggcga ccgcctcaga gctcgcggac
     3541 acagcgcgcg ggtcgctctc caccgcgcag agtgaccgcg ccggcctcgg cgccgctccc
     3601 tcgaccgacc tgctgatagc agcccgctct gccacggaag gccggggcct cgtggagtcc
     3661 ttctacaacg gcaagatcga ctcgccccga ctgtacgggc gtgctctgtc agcgcaggag
     3721 ctcgagcgac tgcgggaagg ctgcgacccg aaggagatcc cggacctcgt ggcggcgtgg
     3781 gacttctcgg ccgatatctc ctccagcagg gtttccgacg tgtcgggcaa cggcttgcac
     3841 ggcgaggccg tgcagcgtcc cggacgggcg gtcaccgggc acaactggga cggccgcgag
     3901 agcaattgga aacactcgcc ggaacagtac ggagccatcc acttccacga cgacgacctc
     3961 gacgacgcgg ggtgggagcg ctcgctgcag tggacggtac ccccggggct cgcgagcggc
     4021 gtgtacgcgc tgcatctcga ggtcccgggc ggtgaggacg acatcccctt cgtcgtgcgc
     4081 ccgaagaccg gacagccgac agcgaagatt ctgttcctca tgccgacgtt cagttacctg
     4141 gcatacggca acgagcacct gctcggcagc ccggcgacac ggagcatgat gatcgacctg
     4201 ggcgtcagcc tcgacacgct cgactacccg tcgcagccgc aggacgagta catcttggag
     4261 aaccggctca gcagcctgta cgactcgcac agcgacggct cgggcgtctt ctactccacg
     4321 cgtatgcggc ccgtcgtcaa catgcggccc aagtacttca tgcagggcct cgacgacgga
     4381 agcggctcgc ctcaccagtt caatgccgac ttgcatctcg tggactggct gcaccagcac
     4441 ggctacgagt tcgacgtcat taccgacgag gacctctatc gcgagggcct cgacgtcctc
     4501 gacggctacg acgtcgtgct caccggcacc caccacgagt actgggcgga gcggatgctc
     4561 gatgcgatga aggcctacct ggacggcggc gggcggctca tgtacctcgc gggcaacggc
     4621 ttctactggg ttaccgagct ggagcccgag cacgggcaca caatcgagat ccgccgtcgc
     4681 ggtccagcca cgcggacgtg ggatgccgcg cccggcgaag gccacctcag cttcacgggg
     4741 gagctcggcg ggctatggcg ctatcgaaac cgcgcacccc aacagctggt cggcgtcggc
     4801 ttcaccgctc aagggaccgg agcgggacgc ccgtacacgc ggcaacccga ctcgttcgat
     4861 ccccgcgcag cattcgtctt cgaaggcgtt ggcgacgacg agttgatcgg ggacttcccg
     4921 tgtctcgtct ccagttacgg cgccgccggc ttcgagctcg accgcgtcga ctacggcctc
     4981 ggcacgccgc atcgcacgat gctgctcgcg acggccacgg ggttctcaga ctcctttcag
     5041 cacgtgagcg aggagatcac tgtcacgacg tccaagcacg gcggccccgc gagcaggctc
     5101 gttcgcgcgg acatggtgct cctcgagtac ccgaacggcg gagccgtgtt ctctccggcg
     5161 tcgatcacct ggtgcggcgc gctcacgtac aacaactacg acaacaacgt gtcgagagtc
     5221 acgcgcaacg ttctcgaccg cttcacttcg ccagggcccg ccattcgcgc ggaagagaag
     5281 ccgctgtaga agacgcgccg cctcgaacgg cgagaaaggg cacccccatc cgtctgccaa
     5341 gccggggcga ctagaggcgg cgagccgcgc ggtgcccctc gtagaccgcc tcgtaggtgc
     5401 cccgcggcgc ggtcgcatcg ccgatcgtct cgacgctcac tccgcgcgcg cggaaagcgt
     5461 ggtagagggc gttgtcggac tgccgtccgg tgttgaagac gatcgcgtcc gcctccaggc
     5521 gggtgacccg ctcgggggca tagacgttga cgaactccac ttcgcggccc gagatccgcg
     5581 ttgcaaacag gtcggtgagg atctccacgc ccgcctccag cagcgcctgg cgggtccaca
     5641 ggtagtacac ctcgggcatc gtctccatcg ccaccatcgg ccagcgagtg acgagtcgca
     5701 cggatgcgcc ctcgcgggcg agcttgactg ccgtcaacgg cccggcgatg tcctgcaggt
     5761 cgtcgatcac gaggatcgag cccgaaaccg ccaccttgcc ttcggcgacc tgatcccagc
     5821 tcgcgcagtt gccgctctcc cagccgggga cggatgcagc agtctggccc tgccagccgt
     5881 cgcgccggta cggcgcgcct gtcgcgacga cgatgtggtc cggtcgctcg tcggtcagca
     5941 gggagtcgag atcggcgtcg ctcacttcga ccccggtgcg aatctcggcg ccgttcttgg
     6001 tcgcctgggc ggcgagccag acggcgatct gtccgagctc ttgccggtcg ggcaagagtg
     6061 cctgcaccct cacgtgtcca ccggcctccg gctcccgctc gagcagcgtc acctcgtgtc
     6121 cccgcgccgc ggcgacgcgg cagaactcca ggccggcggg cccgccgccg atcaccagcg
     6181 cacggcgcgg ctgcgcgaca cgtgtcagcg agccagcgcc ccactggcgc tcgcggccga
     6241 cttcggggtt gtagatgcac tgcacgcgtg caccggcgat cacgttacgc cagcagtagt
     6301 tggccgcgac gcagcgccgg atgtcctcgg gtcggccctc ccggaccttg cgtacccact
     6361 cggcgtcggc gaacagctgc cgcgctagct ggatcgcatc tgcttcgcct gcagcgagaa
     6421 gctcctcggc gatgtccggg ttcgtgatcc gtccgacggc gagcaccggc ttggccgtca
     6481 cctgcttgac ctcgcgcgtg tactcgcgct cccaaccggg ttcttaggtc atcggcgtgt
     6541 ggatgaagga gtgatggacg ccggcggaga cgctcacgta gtcgatgtcc gtcgatcgat
     6601 cgagctcggc gatcacctcc ttgacgtcgt ccatctccag atcgccgggc cagaacgagg
     6661 tcgagttgat tcggtagccg atgaagaggt cgtcgccgac cgcgctgcgc atcgcgcgca
     6721 aggactcgac cgagaagcgg agccggttct cgagtgctcc gccccaacgg tcggagcggt
     6781 ggttgtacag aggggacagg aactgccacg gcaggtaccc gtgcgcgagg tggtactcga
     6841 cgccgtcgag tcccgcctcg ccggcgcggc gcgtcgactc cgcgaagcac ccgatgatct
     6901 cctcgatctc gtgctcctcc agctccttcg gcatcggcgc gtgcgggaac tcgcccggcg
     6961 gaagctggct cggcgcgacc gtgtaccagg gtgcgtcgag gtcgtaagcc gggtccttct
     7021 ggatcactgc cagcgtgcga aggccggggt gcaacagctg cactgccagc ctgcagccgt
     7081 gccgctgcac cgcctggcgc acccgctcca gcccgtctat ctgtgcgtcg tcccagagcc
     7141 ccggaaagtt gagtccgccg tacagcgcgt cgcggtgcac gatcgttccg ccgatgatga
     7201 tcaacccgca cccggccttc gcgcgctctt cgtagtaggc gacggcttga tcggtgaaga
     7261 cgccgtcggc gggattggcc acccacgtcg ggcaggtcgg ggcgaacacc acgcggttcg
     7321 ccatctgagc accgcgcagc ttccattccg agaacagcaa cggatacgtc gtctcggtca
     7381 ccgtcttctc cccgctcgct cggcgccatc atcggtcggg cgagcgaacg cgtcaacacc
     7441 gcggccggct ggtcacgacg cgggccgccg tcgagcggtt cccgcccgag acggcctgct
     7501 cgagtggggc gcccctagag caagtggcgg ccgaagtcgc gctggaaatc gcgccagacg
     7561 ctgtggatgt gagtaccgct gttgcgggag ttgtcgaact cgagcaggaa ggtcgggccc
     7621 tgcagccggt agtagtgcgg caggcccggg cgagtactgc cggcccagcc gaaacgggta
     7681 cgagcgatgc ccgctctatc gacacgcgca agcgcttcgc gcgccatcgc cggcggatag
     7741 ttcgcgaggt aggtacggat gatctcgagc acccgtcttt gggcggacgt ggggagatcc
     7801 cccgcgagca cgccgacccg atcgagcgga cggacgcgca cggcgttcgc cgttacgtgg
     7861 tcggtcagcg attccgatga gaacacgacc cgcttgcgaa gcagtccgtc gagcgagagc
     7921 acgatctcgc gggcggcgtc ctcttcgcgc gccatcgcac gctccccgcg cccgaccgag
     7981 cggtaggcgc tgccggcgcg cgtcggccag gcg
//
LOCUS       DBNGNK010000096         4134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_588419,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000096 DBNGNK010000000
VERSION     DBNGNK010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_588419"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC150V_02600"
     CDS             <1..263
                     /locus_tag="AC150V_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR3761841.1"
                     /translation="APFMSTVTAKIEPAQKLGSLLVDQLTAPVRFTHAAHELMRGGVT
                     TFVEVGPGSVLSGLIKRIDRGVKTISVNDLAGLSKAEEALAR"
     gene            260..988
                     /gene="fabG"
                     /locus_tag="AC150V_02605"
     CDS             260..988
                     /gene="fabG"
                     /locus_tag="AC150V_02605"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR3761842.1"
                     /translation="MSEFCSLDGKTALVTGGSRGIGRAIALELGRAGADVVVGFRSGE
                     AEAQAVASEAGGRAVQADVSDAESARALVEEAGELDLLVNNAGVTRDGLLARMSDDDW
                     RTVLDTNLSSVFFTCRAVARGMMKRRAGSIVNVSSIVGIHGNWGQTNYAASKAGIIGF
                     TKSLARELGSRNVRANVVAPGYVTTRLTELLPEEATQAMLDNTPLGRLGEPEDVAGAV
                     RFLCSDEASFVTGEVLLVDGGLGM"
     gene            1003..2262
                     /gene="fabF"
                     /locus_tag="AC150V_02610"
     CDS             1003..2262
                     /gene="fabF"
                     /locus_tag="AC150V_02610"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR3761843.1"
                     /translation="MSDQNGRRRVVVTGMGMVTPLGNDLESTWTALLAGESGAGPITQ
                     FDSDGYGVHFACEVKDFDATDWIDRKSARRMDRFTHLALAAARMAEADAELDVSKEPQ
                     RVGAAVATGIGGLKSFETCMDNLYSRGPDRTNPFSIVQIIPNLAAGWVSMELGTQGPL
                     LAQCTACAASNMAIGDSLDAIRLGRADAMICGGTEAPVSKVGIAGFNAMRALSRRNDD
                     PKRASRPFDAERDGFVMGEAGAMIVIEELEHAKARGARIHAELLGYGVSSDAAHVSDP
                     DPTGENPARALSMAFADAGVDPAQTDYVNAHGTSTPVGDPAEVRVLKLALGEEKALTT
                     PVSSTKGATGHCLGAAGAVEAIFTVLATKSGMVPPTINQETPDPECDLDFIPNEARPH
                     DVEIGISNSFGFGGHNACVVFRRWRDD"
     gene            2269..2928
                     /locus_tag="AC150V_02615"
     CDS             2269..2928
                     /locus_tag="AC150V_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR3761844.1"
                     /translation="MDRWATFDCYGTLVDWQAGIGAELTRLFGKSEAETLLLRYHALE
                     PDVQRDTRLAYRDVLARTLSLLAAEAGEKLPAGEEDALARSLPSWPVFADAAPALAEA
                     RRRGWRLAILSNSDRDLIDASTGAIGIPFDYVVVAGEIGSYKPHPRHWEVFAAASEAR
                     HERHVHVAQSLFHDIDPAHLLGLRSVWINRLGEPADPRPTWVLPDLAELPDTLEKLVS
                     A"
     gene            2931..3860
                     /locus_tag="AC150V_02620"
     CDS             2931..3860
                     /locus_tag="AC150V_02620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3761845.1"
                     /translation="MRRPTEADREGVLALLQAADVAVLGETDWTERDLRSQWETLDLT
                     RDAWLVELDGALAGYATFEVRGGGRLMADGYVEPGLRGRGVGGTLLRLTEERALLELA
                     RAGPGKRVYLQNAALLDEDSATPELYAEHGYAPVRHFWRMVTDLPPDEPPPPEIPPGL
                     RLDAFDRDRDARALHSAHQETFAEHWEHRPQSWEAWVEDWLGDERCDPTLWLVARAGD
                     EIAGFAVNDWKTEGDSGYVRSLGVRPGWRRRGLGEALLRVSFREFHRRGERRVTLGVD
                     AQNASGATRLYERVGMRVLWRAAVYEKEVRPGR"
     gene            3898..>4134
                     /locus_tag="AC150V_02625"
     CDS             3898..>4134
                     /locus_tag="AC150V_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761846.1"
                     /translation="MSTLRARCPDCRTLTAVALGPEYQCHSCGREYAAGLVRVPRAWG
                     VGGEAMAEAAWLPLPYPETGVIDHDTLEGQSRALA"
BASE COUNT          642 a         1362 c         1498 g          632 t
ORIGIN      
        1 tcgctccgtt catgtcgacc gtgacggcga agatcgagcc ggcgcagaag ctcgggtccc
       61 tactggtcga tcagctgaca gcgccggtcc gcttcaccca cgctgcgcac gagctcatgc
      121 gcggcggcgt gacgacattc gtggaagtgg ggcccggcag cgtgctgtcg ggcctgatca
      181 agcgaatcga tcgcggcgtg aagacgatct cggtcaacga tctcgccggg ctgtccaagg
      241 cagaggaggc gctagcgcgt tgagcgagtt ctgctcgctc gacggaaaga ccgcgctcgt
      301 caccggaggc tcgcggggaa tcggccgggc gatcgcgctc gagctcgggc gggcgggagc
      361 cgacgtagtc gtcggctttc gcagcggcga ggccgaggcg caagcggtcg cctcggaagc
      421 gggcggccgc gcagtccagg ccgacgtttc cgacgcggaa tccgcacgcg cgctggtcga
      481 ggaggccggt gagctcgatc tcctcgtgaa caatgccggc gtcacgcgag acggcctgct
      541 ggcgcgcatg tccgacgacg actggcgcac ggtgctcgac accaacctct cctcggtctt
      601 cttcacctgc cgcgctgtgg cgcgcgggat gatgaagcgt cgagccgggt cgatcgtgaa
      661 cgtgtcgtcg atcgtcggca tccacggaaa ctgggggcag acgaactacg cggcctcgaa
      721 ggccggaatc atcggcttca cgaagtcact ggcgcgcgag ctcggcagca ggaacgtccg
      781 agcgaacgtc gtagcgcccg gatatgtgac aacgcggctg accgagttgc tgcccgagga
      841 ggccacacag gcgatgctcg acaacacccc actgggtcga ctcggcgagc cggaggacgt
      901 tgccggtgcg gtgcggttcc tgtgctcgga cgaggcctcg ttcgtcacgg gcgaggtact
      961 gctcgtcgac ggcggcctcg ggatgtagcc tcacccggtg ggatgagcga ccagaacggc
     1021 aggcggcggg tggttgtcac cggaatgggg atggtgacgc cgctcggcaa cgacctggaa
     1081 tcgacgtgga ccgcgcttct ggccggcgag tccggtgccg gtccgatcac acagttcgac
     1141 tccgacggct acggagttca cttcgcctgc gaggtcaagg acttcgacgc gacggactgg
     1201 atcgaccgga aatcggctcg ccgaatggac cgcttcacgc atctggcgct cgcagccgcg
     1261 cgtatggccg aggcggatgc cgagctcgac gtctcgaagg agccccagcg cgttggagcc
     1321 gccgtcgcga cgggtatcgg aggcctgaag tccttcgaga cgtgcatgga caacctctac
     1381 tcccgcggcc cggaccggac gaaccccttc tcgatcgtcc agatcatccc gaatctcgcc
     1441 gccggctggg tctcgatgga gctcggcacg cagggaccac tgctggccca gtgcaccgcg
     1501 tgcgcagcgt cgaacatggc gatcggcgac agcctcgacg cgatcaggct ggggcgcgcg
     1561 gacgcgatga tctgtggcgg aaccgaggcg ccggtcagca aggtcggcat cgcaggtttc
     1621 aacgccatgc gggcactctc gcggcggaac gacgacccga agcgcgcatc gcgccccttc
     1681 gacgcggagc gggacgggtt cgtgatgggc gaggcgggcg cgatgatcgt gatcgaggaa
     1741 ctcgagcatg cgaaagcacg aggtgcgcgc atccacgccg agctgctcgg ctacggggtc
     1801 tcctcggatg cggcgcacgt ctccgacccc gatccgaccg gtgagaaccc ggcccgggcg
     1861 ctgagcatgg cgttcgccga cgcgggcgtc gaccccgcac agaccgacta cgtcaacgcc
     1921 cacggcacgt ccacgccggt cggcgacccc gccgaggtgc gcgttctgaa actggcgctc
     1981 ggcgaggaga aagctctgac gacgccggtg tcgtcgacga aaggagcaac cggtcattgc
     2041 ctcggggccg cgggcgctgt cgaggcgatc ttcaccgtgc tcgcgacgaa gagcgggatg
     2101 gttcccccga cgatcaacca ggagaccccc gacccggagt gcgatctcga cttcataccg
     2161 aacgaggcgc gcccgcacga cgtcgagatc ggtatctcga actcctttgg gttcggcgga
     2221 cacaacgcgt gcgtcgtctt tcgtcgctgg cgcgacgact agtccggggt ggatcgctgg
     2281 gcgacgttcg actgttacgg gacgctcgtc gattggcagg cggggatcgg agccgagctg
     2341 actcggctct tcgggaagag tgaggcggag acgcttctct tgaggtacca cgcgctcgag
     2401 cccgacgttc agcgcgacac gcgcctcgcg taccgcgacg tgctcgcgag gacgctgtcg
     2461 ctgctggcgg ccgaggccgg tgagaagctg ccggctggag aggaggatgc gctcgcgcgc
     2521 tcgttgccga gctggccggt cttcgccgac gctgcgccag cgctcgccga ggcccgtcga
     2581 cgcggctggc gcctggcgat cctctccaac tcggaccgcg acctgatcga cgcgtcgacg
     2641 ggggccatcg gcatcccctt cgactacgtg gtcgtggcgg gggagatcgg ctcctacaag
     2701 ccgcatccgc ggcattggga agtctttgcc gcggcgagcg aggcgcgcca cgagcgccat
     2761 gtgcacgtgg cgcagagcct gttccacgac atcgacccgg cgcacctgct tgggctacgg
     2821 tcggtctgga tcaatcggct cggtgagccc gccgatccgc gtccgacctg ggtgcttccc
     2881 gatctggccg agctcccgga cacgctcgag aagctcgttt cggcgtgatc gtgcggcgac
     2941 ctacagaggc cgatcgagag ggcgttctcg cgctcctcca ggccgccgac gtggcggtgc
     3001 tcggcgagac ggactggacc gagcgggatc tccggagcca gtgggagacg ctcgacctga
     3061 cgcgggacgc atggctcgtc gagcttgacg gcgccctggc aggctatgcg acgttcgagg
     3121 tgcggggcgg cggtcgactg atggcggacg gctacgtcga gccggggctt cgcggacgcg
     3181 gtgtcggcgg caccctgctc cgcctgaccg aagaacgggc tctgctggag ctggctcgag
     3241 ccggccccgg caagcgcgtc tacctccaga acgccgcgtt gctggacgag gactcggcga
     3301 cgcccgagct ctacgccgag cacggctatg cgccggttcg ccacttctgg cggatggtga
     3361 ccgacctccc tccggacgag ccgccccctc cggagatccc gcccgggctc agactcgacg
     3421 cgttcgaccg cgaccgtgac gcccgtgcgc tgcattctgc gcaccaggag acgttcgccg
     3481 agcactggga gcaccgaccg caatcgtggg aggcgtgggt ggaggactgg ctcggtgacg
     3541 agcgctgcga ccccacgctc tggctcgtcg cccgcgccgg cgacgagatc gccgggttcg
     3601 ccgtcaacga ctggaagaca gagggagact ccggctatgt gcggtcactt ggtgtgcggc
     3661 ccggctggcg acggcgaggg ctcggggagg ctcttctccg cgtgtcgttt cgcgagttcc
     3721 accggcgcgg cgagcgccgt gtcaccctcg gggtggacgc gcagaacgcg agcggcgcga
     3781 cgcgtctgta cgagcgcgta ggcatgcgcg tgctgtggcg cgcggccgtg tacgagaagg
     3841 aagttcgccc tggccgttga ggcatcggtt gcggccgtcg agaggcgggc ctgagccgtg
     3901 tccaccctgc gtgcacgctg cccggactgc cgaacgctga cggcggtcgc cctcgggccg
     3961 gagtaccagt gtcactcctg tggccgggag tacgccgcgg gactcgtacg cgttccccgc
     4021 gcgtggggag tcggcggcga ggcgatggca gaggccgcgt ggcttccgct tccctacccg
     4081 gagacggggg tgatcgacca cgacacactc gaaggtcaga gccgcgctct cgcc
//
LOCUS       DBNGNK010000097         7673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1092131,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000097 DBNGNK010000000
VERSION     DBNGNK010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1092131"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4365
                     /locus_tag="AC150V_02630"
     CDS             <1..4365
                     /locus_tag="AC150V_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR3761847.1"
                     /translation="LYKLSLVFPDRAPSRFRAALKAGSVASLEKRLEEAKAAGSSSTP
                     LEAAERLLALVAALDSHDKLTRGHADRVRAYAQMIGQELGLSSEDVDRLNWAALLHDI
                     GKLEVPHEILQKPGRPTDDEWAVLARHPEFGSELVVPLREWLGEWAEAVPQHHERWDG
                     KGYPNSLAGEEIALGGRIVAVADVFDVITSARSYKDASDPASGRAEIARCAGEQFDPR
                     VVRAFLGISLGRLRFAMGPLSWLAHAPILARLPLTPALGSMSAAAGAVVVAVSAGVVA
                     ESAVTPPNLTQAPIARTVVQPPQPAAPTPSPQPPPNPAPPQRPSQDEPSPPPSTPPGD
                     RDLVAAEPANRAPVATPDEVSTAEDTPVTIPVLANDSDPDGDPVGLVAVEPPEMGWVQ
                     RAGAALTYTPPPNASGEAAFRYTAGDGRGGRATANVTVTITAANDAPVAENDSASARA
                     GESVTVDVLANDHDVDSDELRLVRVGAPAVGTVSRDGDDVLYTAPAAASGATSFSYTV
                     ADAAGATAQATVDVALMAVNSPPAFTLGRDPTVLENAGPQRLTGWATGIRPGPPHEAG
                     QSVRFGTATPSPELFAAGGRPVLERDGSLTFTPAKNTSGAAEVTVVATDDGGVANGGS
                     DTSEPQTFTITVLPVNTPPSFTIGPDQKVATTGGPQVVKRWATAISAGPADESDQKLR
                     FVVTLDDPSLFVAGGQPAVSADGTLAYTPAKGAGGTAIVTVRAVDDGGSANGGSDTSA
                     PQTFTITVVAVNTPPSFVAGGDQSVFENAGLQTVANWATAISAGPPEDARQTISFTVT
                     TDKPSLFAAGGRPAVSSAGTLTYTTAPGANGTAVVTVRAVDDGGTELGGNDTSPPQTF
                     RIVVAAVNSAPSFTLGASPSVLENAGPQTIAAWATAISAGPPDEAGQSVTFLVSSDNA
                     PLFTTAPSISPTGTLTFHAAPNASGTATVTVVAKDDGGTANGGQNQSAPQTFTITVGE
                     VNTAPSFTGGPSKTVAEDAGPYTAAGWATAIDPGPGEGGQTVSFTVTNDNNGLFSAQP
                     AVASDGTLTFTSAPNAFGSATVTVRAVDDGGTANGGSDTSAPQVFTITVTSVNDPPVA
                     VDDAAAVDEDTTGGVTFGVLGNDTDVEGDTLSLDSFDGSTIANGTLTDNGGGSFTYVP
                     DSDYNGAESFTYVVGDGNGGTDTGTVTITVVAQPDAPTAASDAYSTQQDTALVEAAPG
                     VLANDADEDGDPLVVQTTPVVGPTSGTLSLAADGSFTYTPNGGFVGTDSFTYRVDDGT
                     GLSADAVVTITVSAVSVPSSLYMHPTGASADVWDLQTTPPPAAPPVPDHDGDGFPGRS
                     IKHSAGDEDNSDPREWQEWSYAVASPLVLDGPVKLQLWSTISYFELNKDAHPHIYLYD
                     CDAAGASCVKLAETDIHVDDWNGLIPSWVFHEFTIGSVSQTIASGRTLRVRLLIEHED
                     LWVALTAGYPSALVVTTG"
     gene            complement(4373..4870)
                     /gene="paaD"
                     /locus_tag="AC150V_02635"
     CDS             complement(4373..4870)
                     /gene="paaD"
                     /locus_tag="AC150V_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HIR3761848.1"
                     /translation="MVVASPSENDVWRALGEIPDPEIPVISLVELGVIKDVAVGGGRV
                     TVEFTPTFMGCPALDHMQRQIRERVEALGAECEVKVLHDDSWSTDLITAAGRRKLQEA
                     GFAPPPRRSEGAVTLVQLQRGGHRCPYCSSSETRLDNIFGPTPCRSLRYCESCRQPFE
                     QFKTI"
     gene            complement(4864..5544)
                     /gene="paaC"
                     /locus_tag="AC150V_02640"
     CDS             complement(4864..5544)
                     /gene="paaC"
                     /locus_tag="AC150V_02640"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR3761849.1"
                     /translation="MPARRTRAELLLELADDELVLGWRDSEWTGIAPFLEEDVAFSSI
                     AQNEIGHARALYEVAAAELGTTADELAFDRRPQEYRCAPLVELRRLEWARTIARHWLY
                     ETADALRLETLKGSDDGEIAALAAKMDREELYHRMHADMWLERLLSSDEGRGRLDEAL
                     TELWPYALGVLDEELRPEYVRRVEARLGRSMPDAEPVPRGRHEAELTELWDEMTMVRR
                     SAPQGARW"
     gene            complement(5519..5806)
                     /locus_tag="AC150V_02645"
     CDS             complement(5519..5806)
                     /locus_tag="AC150V_02645"
                     /inference="COORDINATES: protein motif:HMM:NF018002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaB"
                     /protein_id="HIR3761850.1"
                     /translation="MSEVWEVFRRERHGQPFQHAGAVRAPDARFAEIYAREQYGRRQE
                     SDALWLVPRAEIHEITEFPDEFDLKYRRVDGYSIKARLREARERAGTSHEG"
     gene            complement(5803..6702)
                     /gene="paaA"
                     /locus_tag="AC150V_02650"
     CDS             complement(5803..6702)
                     /gene="paaA"
                     /locus_tag="AC150V_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR3761851.1"
                     /translation="MDRRTQEFLEHVQSGGQVEATDWLPEEYRKRLIKFVEMHANSEL
                     MGVLPERDWILRAPTLQRKLALTAKIQDEVGHAQLIYRVVEDLGKPREQCLGDLLAGT
                     SKFHNVFHYPTRSWGDVGVIAWLVDAAAIISQKALLKCSYAPYARIMKKICWEESFHV
                     LHGRDVVLALVTGTEEQRELVQEAIDRWWAPLMQFHGTPVPADEDPMVRWRIKSQANE
                     DARQQFLDGYVPQVWELGLTLPDPKLRRREDGVWEYTEPDWEELMSVVTGHGPASQER
                     LAFRRLSRSEVDWVSSLVLAEAA"
     gene            6746..7321
                     /locus_tag="AC150V_02655"
     CDS             6746..7321
                     /locus_tag="AC150V_02655"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3761852.1"
                     /translation="MATRRDELVATAARMFAEQGYHGTSMGEIADAMGVRKASLYSLI
                     ESKERLLYESTRAGADAFHAALDGVPEDVAAAERIRLALRAHLRVVSEQLEVATVFTR
                     EWRYLDGPARDEFLAERRRYQERWRGLFREGVEHGELRTDLDVSAATLLVLSAANWAY
                     TWLRAGIDTDDLADRFTEILVDGVRGYSTPA"
     gene            7318..>7673
                     /locus_tag="AC150V_02660"
     CDS             7318..>7673
                     /locus_tag="AC150V_02660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3761853.1"
                     /translation="MRRLLARAALATWSWAVLVAVLDRRHGLSLDDVAPKPDGPLVSV
                     IVPARDEERAVRRAVQSLAGQDYPNLEVIVVDDESSDATAAEARSVSSSRVRVLDGRP
                     LPPGWVGKSWACHEGAR"
BASE COUNT         1162 a         2811 c         2596 g         1104 t
ORIGIN      
        1 ctctacaagc tctcgctcgt tttcccggac cgtgcgccct cgcgcttccg cgccgccctc
       61 aaggccggaa gcgtcgcgag cctggagaaa cggctggagg aggcgaaggc tgcgggctcg
      121 agctccacac cgctcgaggc ggccgagcgg ctgctcgccc tcgtcgccgc gctcgactcg
      181 cacgacaagc tcacgcgcgg ccatgctgac cgcgtccgcg cctacgcgca gatgatcgga
      241 caggagctcg gtctctcgtc cgaggacgtc gaccgcctaa actgggccgc gcttctgcac
      301 gacatcggca agctcgaagt gccgcacgag atcctccaga agccgggtcg tccaaccgac
      361 gacgagtggg cggtcctcgc gcgacacccg gagttcggct cggagctcgt cgtgccgctt
      421 cgggagtggc tcggcgaatg ggccgaagcg gtgccccagc accacgagcg ctgggacggg
      481 aaggggtatc ccaacagcct ggccggagag gagatcgcgc tcggcggccg gatcgtcgcc
      541 gtcgccgacg tcttcgacgt catcacctcg gcacggtcgt acaaggatgc ctccgacccc
      601 gcctcgggtc gcgccgagat cgcccgctgc gccggcgagc agttcgatcc cagagtcgtg
      661 cgggccttcc tcggcatctc gctcgggcgc ctgcgctttg cgatgggccc gctctcgtgg
      721 ctcgcacacg caccgattct ggcccggctg ccgctgacgc cggcgctcgg ctcgatgtcg
      781 gctgccgccg gcgctgttgt cgtcgcagtc tcggcgggcg tggtcgcgga gtccgcagtg
      841 actcctccca atctgacgca ggcgccgatc gcccgcacgg tagtccagcc gcctcagccc
      901 gccgccccga cgccgagccc gcagccgccg ccgaatcctg cgccgcccca gagaccgtcc
      961 caagacgagc cgagcccgcc gccgagcact cccccgggtg accgcgacct cgtcgcagcc
     1021 gagcctgcga accgtgcacc tgtcgccact cccgacgagg tctcgacggc cgaggacacg
     1081 ccggtgacga tccccgttct cgcgaacgac tcggaccccg acggcgatcc ggtcgggctg
     1141 gtcgccgtcg agccgccgga aatgggttgg gtccagcgcg ccggagctgc tctcacctac
     1201 acaccgccgc ccaacgcgtc gggcgaggcg gcgttccgct acacggccgg ggacggccgg
     1261 gggggcaggg cgaccgcgaa cgtgaccgtg acgatcacgg cagcgaacga tgctcccgtc
     1321 gccgagaacg actcggccag cgcccgcgca ggcgagtcgg ttaccgtcga cgtgctcgcc
     1381 aacgaccacg acgtcgactc cgacgagctc cgactcgttc gcgtcggggc gcctgccgtc
     1441 ggcaccgtgt cacgagacgg agacgacgtg ctctacacgg cgccggccgc cgcgagcggg
     1501 gcgaccagct tctcctacac cgtcgcagac gccgccgggg cgacggctca agccaccgtc
     1561 gacgtagcgc ttatggcggt caactctcct ccggctttca cgctcgggag agatcccacc
     1621 gtgctcgaga acgccggccc tcagcggctc accggctggg cgacgggcat ccggccgggc
     1681 ccgccgcacg aggcggggca gagcgtccgg ttcggcaccg cgacgccctc gcccgagctg
     1741 ttcgccgccg gcggccgacc tgttctcgaa cgtgacggct cgctgacctt cacgccggcg
     1801 aagaacacga gcggcgccgc cgaagtgacc gtcgtcgcga ccgacgacgg tggggtcgcc
     1861 aacgggggca gcgacacgag cgagccgcag accttcacga tcaccgtcct gccggtgaac
     1921 acgccgccgt cgttcacgat cggaccggac cagaaggtgg ccacgacggg cgggccgcag
     1981 gtcgtgaagc gctgggcgac cgcaatcagc gccggccccg ccgacgaaag cgatcagaag
     2041 ctgcgcttcg tggtcacgtt ggacgatccg tcgctcttcg tggccggcgg ccaaccggcc
     2101 gtctctgcag acgggacgct tgcctacacg cccgccaagg gcgccggggg gacggcgatc
     2161 gtcaccgtcc gtgcggtcga cgacggcggg tcggcaaacg gtggctccga cacgagcgcc
     2221 ccgcagacgt tcacgatcac cgtcgtggcc gtgaacacgc cgccgagctt cgtcgcgggt
     2281 ggcgaccagt cggtgttcga gaacgcgggg ctgcagacgg tcgcgaactg ggcgaccgcg
     2341 atcagcgccg gcccgcccga ggacgcgcgt cagacgatca gcttcaccgt cacgaccgac
     2401 aagccgtcgc tcttcgcagc aggcggccga cccgccgtct cctccgccgg cacactcacc
     2461 tacacgacgg cgccgggcgc caacggcacg gccgtcgtca ccgtccgcgc cgtcgacgac
     2521 ggaggtaccg agctcggcgg caacgacacg agcccgccgc agacgttcag gatcgtcgtc
     2581 gcggccgtga actcagctcc ctccttcacg ctcggtgcgt cgccgtcggt actcgagaac
     2641 gccggcccgc agacgatcgc cgcgtgggcg actgcgatca gcgccgggcc ccccgacgaa
     2701 gccggccagt cggtgacgtt cctcgtcagc tcggacaacg cgccgctgtt caccaccgca
     2761 ccctcgatca gcccgaccgg cacgctcacg ttccacgccg cgcccaacgc cagcggaacg
     2821 gcgacggtga ccgtcgtggc gaaggacgac ggcggcacgg cgaacggagg ccagaaccag
     2881 agcgccccgc agacgttcac gatcacggtt ggtgaggtca atacagcccc gtctttcact
     2941 gggggcccga gcaagacggt cgccgaggat gccgggccct acacggccgc aggttgggca
     3001 accgcgatcg acccgggtcc cggcgaaggg gggcagacgg tcagcttcac ggtcacgaac
     3061 gacaacaacg gactcttctc ggcccagccg gccgtcgcct cggacgggac cttgaccttc
     3121 acgtcggccc cgaacgcgtt cggctcggcg acggtgacgg tgcgcgccgt cgacgacggc
     3181 ggcactgcga acggtggctc cgacacgagc gcgccccagg tcttcacgat caccgtcacc
     3241 tcggtcaacg acccgccggt cgcggtggac gacgcggccg cggtggacga ggacacgacg
     3301 ggcggcgtca cgttcggcgt gctcgggaac gacacggacg tcgagggcga cacgctgtcg
     3361 ctcgattcct tcgacggctc gacgatcgcg aacggcacgc tgacggacaa cggcggcggc
     3421 agcttcacct acgtgcccga ttccgactac aacggcgccg agtcgttcac gtacgtcgtc
     3481 ggcgacggaa acggcggcac cgacacgggt accgtgacca tcacggtcgt cgcccagccc
     3541 gacgccccca ccgcggcgtc ggacgcgtac tcgacccagc aggacacagc gctcgtcgaa
     3601 gccgcgccgg gcgtgttggc gaacgacgcc gacgaggacg gcgacccgct cgtcgtgcag
     3661 acgacaccgg tggtaggtcc cacgagcggc acgctctcgc tcgccgccga cggctccttc
     3721 acgtacaccc cgaacggcgg cttcgtcggc acggactcgt tcacctaccg tgtcgacgac
     3781 ggcaccggcc tatccgcgga tgccgtcgtc acgatcaccg tctcggcagt gagcgttccc
     3841 tcgtcgctct atatgcaccc gaccggcgcc tctgcggacg tctgggatct tcagaccacg
     3901 cccccgcctg ccgccccccc cgtgcccgac cacgacggcg acggctttcc agggcgcagc
     3961 atcaagcaca gcgccggcga cgaggacaac tccgacccgc gcgagtggca ggagtggtcg
     4021 tacgccgtcg cctcgccgct cgtcctcgac gggcccgtga agctgcagct ctggagcacc
     4081 atctcctact tcgagctgaa caaggacgca cacccgcaca tctacctcta cgactgcgat
     4141 gccgccggag cgagctgcgt gaagctcgcc gagaccgaca tccacgtcga cgactggaac
     4201 gggttgatcc cgagctgggt cttccacgag ttcaccatcg gctccgtcag ccagacgatc
     4261 gcctccgggc ggacgctgcg cgtgcggctc ctcatcgagc acgaggatct ctgggtggcg
     4321 ctgaccgccg gctacccgtc cgcgctcgtc gtgacgaccg gctgaggacc gcctagatcg
     4381 tcttgaactg ctcgaagggc tggcggcagc tctcgcagta gcgcagcgac cggcacgggg
     4441 tcgggccgaa gatgttgtcg agacgggtct cgctcgagct gcagtagggg cagcggtggc
     4501 cgccacgctg gagctgcacg agcgtcacgg cgccctccga ccggcgtggc ggcggcgcga
     4561 agccggcctc ctgcagcttt cgccggcccg cggccgttat cagatcggtc gaccaggagt
     4621 cgtcgtgcag taccttcacc tcgcattcgg cgccgagcgc ctcgacccgc tcgcggatct
     4681 ggcgctgcat gtggtcgagc gcggggcagc ccatgaacgt gggcgtgaac tcgacggtga
     4741 cgcgtccgcc ccccacggcg acgtccttga tcacgccgag ctcgaccagc gagatgaccg
     4801 ggatctcggg atccgggatc tcgcccagcg cccgccagac gtcgttttcg ctgggggagg
     4861 ccactaccac cgggccccct gcggagcgga tcggcgcacc atcgtcatct cgtcccagag
     4921 ctcggtgagc tcggcctcgt gccggccgcg cggcacgggc tcggcgtcgg gcatcgaccg
     4981 gcccaagcgc gcttcgacgc gccgcacgta ctccgggcgt agctcctcgt cgagcacgcc
     5041 gagcgcgtac ggccacagct cggtcagcgc ctcgtcgagc cggccgcgcc cttcgtcaga
     5101 ggagaggagc cgctcgagcc acatgtctgc gtgcatgcgg tggtagagct cctcgcgatc
     5161 catcttcgcc gcgagcgccg cgatctcgcc gtcgtcggag cccttcaggg tctcgagccg
     5221 cagcgcgtcg gccgtctcgt acaaccagtg gcgcgcgatc gtccgtgccc actcgagccg
     5281 gcgcagctcg acgaggggcg cgcaccggta ctcctggggg cggcggtcga aggcgagctc
     5341 gtccgcggtc gtcccgagct cggccgccgc cacctcgtac aacgcgcgcg catgcccgat
     5401 ctcgttctgg gcgatcgagg agaaggcgac gtcctcctcg aggaacggtg cgatgccggt
     5461 ccactccgag tcgcgccagc cgaggacgag ctcgtcgtcc gccagctcga ggagcagctc
     5521 agccctcgtg cgacgtgccg gcacgctctc gcgcctcccg caaacgagcc ttgatcgagt
     5581 agccgtcaac gcgtcggtac ttcaggtcga actcgtcggg gaactccgtg atctcgtgga
     5641 tctcggctct cgggacgagc cagagcgcat ccgactcctg gcgtcgcccg tactgttccc
     5701 gcgcgtagat ctcggcgaac cgcgcgtcag gtgcgcgcac tgcgccggcg tgctggaacg
     5761 gctgcccgtg ccgctcgcgg cggaacacct cccagacctc gctcatgccg cctcggcgag
     5821 aacgaggctc gagacccagt cgacctcgct gcgcgagagg cggcggaagg cgagccgctc
     5881 ctgcgaggcg gggccgtggc cggtcacgac cgacatcagc tcctcccagt cgggctccgt
     5941 gtactcccag acgccgtcct cacggcggcg cagcttcggg tcgggcaggg tcaagcccag
     6001 ctcccatacc tgcgggacgt acccgtcgag gaactgctgg cgtgcgtcct cgttcgcctg
     6061 gctcttgatc cgccagcgca ccatcggatc ctcgtcggcg ggaacgggcg tgccgtgaaa
     6121 ctgcatcagg ggagcccacc agcggtcgat cgcctcctgc acgagctctc gctgctcctc
     6181 cgttccggtc acgagcgcga gcacgacgtc ccggccgtgc agcacgtgga aggactcctc
     6241 ccagcagatc ttcttcatga tgcgcgcgta cggtgcgtac gagcacttca ggagcgcctt
     6301 ctggctgatg atcgcggccg cgtcgacgag ccacgcgatc acccccacgt cgccccagga
     6361 gcgggtcgga tagtggaaga cgttgtgaaa cttggaggtg cccgcgagaa ggtcgccgag
     6421 acactgctcg cgcggcttgc cgagatcttc gaccacgcgg tagatcaact gcgcgtgccc
     6481 gacctcgtcc tggatcttcg cggtcagtgc gagcttccgc tgcagcgtcg gggcgcggag
     6541 gatccagtcg cgctccggca acacgcccat cagctccgag ttggcgtgca tctcgacgaa
     6601 cttgatcagg cgcttgcggt actcctcggg gagccagtcc gtcgcctcga cctggccgcc
     6661 cgactggacg tgctcgagga actcctgcgt gcgcctgtcc atacgagagt ggtcgtgcta
     6721 cctaccggtc gttagcatcg agtatatggc aacgaggcgg gacgagctcg tcgcgaccgc
     6781 ggccaggatg ttcgcggagc agggctacca cggcacgtcg atgggcgaga tcgccgacgc
     6841 gatgggcgtc cggaaggcct ccctgtactc actgatcgag tcgaaggagc ggttgctgta
     6901 cgagtcgacc cgtgcgggcg ccgacgcctt ccacgcggcg ctcgacggcg tgcccgagga
     6961 cgtggcggcg gccgagcgca tccgcctcgc gctgcgggcg cacctgcgcg tcgtgtccga
     7021 gcagctcgag gtggcaacgg tgttcactcg cgaatggcgg tatctcgacg gtccggcccg
     7081 ggacgagttc ctggccgagc gtcgccgcta tcaggagcgg tggcgcggcc tcttcaggga
     7141 gggcgtcgag cacggcgagc tccgtaccga cctcgacgtg tcggccgcga cgctgctcgt
     7201 cctgtcggct gcgaactggg cctatacgtg gctccgtgcc ggcatcgaca ccgatgacct
     7261 ggcagaccgc ttcacggaga tcctggtcga cggcgtccgc ggctattcga cgcctgcatg
     7321 aggcgactcc tcgcccgcgc cgcactggcg acgtggagct gggccgttct cgtcgccgtg
     7381 ctcgaccggc ggcatgggct gtctctcgac gacgtcgcac cgaagcccga cggtccgctc
     7441 gtctcggtga tcgtgcccgc ccgagacgag gagcgcgccg tgcgccgcgc cgtgcaatcg
     7501 ctggccggcc aggactaccc gaacctcgag gtgatcgtgg tcgacgacga gtcgagcgac
     7561 gccacggccg ccgaggcgcg cagcgtttcg tcctcccggg tccgagtcct ggacgggagg
     7621 ccgcttccgc cggggtgggt cggcaagtcc tgggcgtgtc acgagggcgc ccg
//
LOCUS       DBNGNK010000098         7228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_375613,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000098 DBNGNK010000000
VERSION     DBNGNK010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_375613"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /gene="argH"
                     /locus_tag="AC150V_02665"
     CDS             complement(<1..866)
                     /gene="argH"
                     /locus_tag="AC150V_02665"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR3761854.1"
                     /translation="MSLWAGRVGGSLDPAVWEFLRANDAELLPYDCEATVEHAGRLHA
                     AGLLTGAELEEAETRLADLAQDPSAYLSSDEDVHTAIERVLGDVGRKIHAGRSRNDQV
                     AAAFRLYVLDACAEAREEIDALALVVLSFAEAEADTLMPGYTHLQRGQPVTLGHHLLA
                     WVEMLERDRIRFAAAADASAESPLGAGALAGSTLDLPPPPRQMRNSIDAVSDRDFALD
                     YLYAAAVLFTHLARIGEEIVLWATAEFGFARLPEAAATGSSMMPQKLNPDVAELVRGK
                     AGTAIGRLAGLLA"
     gene            complement(863..2071)
                     /locus_tag="AC150V_02670"
     CDS             complement(863..2071)
                     /locus_tag="AC150V_02670"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR3761855.1"
                     /translation="MPGTAVVAFSGGLDTSCIIAWLKEEWYGFDHVVAVLVDVGQHFD
                     IDESMARGRAAGADDVLLVDRRDAFADDQCAKAIQTNALYEGKYPLVSSLSRPVIAQA
                     SAEVALDAGAEALVHGCTGKGNDQLRFELAFKALDPAARVIAPLRDRIWTRDEEIEYA
                     IAKGIPVKQTAESPYSIDENLFGRAIEAGVLEDPWNAPPRDPYEYTKDPNMAPMPVEI
                     VVGFDRGVPVTLDGEELPLAALIMRLNTLAGEYGIGRIDMIENRAVGIKSREVYEAPA
                     AMTLIAAHSALEDVILTKDEARTKRHLEQRWTELVYEGLWFHPAREAIDAFVAKTQEL
                     VTGEVRLELRANTAVVTGRRSEHMLYAEQLASYGAGETFPHGAAEGFIQIAALETELA
                     AARARAKAKA"
     gene            complement(2071..2538)
                     /locus_tag="AC150V_02675"
     CDS             complement(2071..2538)
                     /locus_tag="AC150V_02675"
                     /inference="COORDINATES: protein motif:HMM:NF013483.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0034618 - arginine binding [Evidence IEA];
                     GO_process: GO:1900079 - regulation of arginine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR3761856.1"
                     /translation="MNRRERQARILELVQERAVSTQSELAELLRGEGFEVVQTTVSRD
                     VGELGLVKVRAPSGRLVYAAPGASDADRLRAIGSAMRRYALTVEAAGGGLVVVTTPAG
                     YASALAQVIDEGEHPSIVGTLAGDNAIFIASRRGVSASALRDELAEHLMEGAA"
     gene            complement(2601..3770)
                     /locus_tag="AC150V_02680"
     CDS             complement(2601..3770)
                     /locus_tag="AC150V_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3761857.1"
                     /translation="MTRAGNSPVLAELQDRLLPTYARANVTIVRGEGCRVWDDTGRAY
                     LDFVAGIAVVGLGHLHPSPLEAAHAQLDRLWHSSNLYWSEPMAALASALSSRMGGARA
                     FFCNSGAEANEAALKYARKATGRPGIVAIEGGFHGRTLGALSVTGQPPKWEGFGPLVP
                     GVAFSRPNEVESLEAAVTPGGDIAAILLEPILGEGGVVPLERGFATAAAEIAREVGAL
                     LVLDEVQAGMGRTGTFFAYEQLGLQPDAVTLAKGLANGLPIGALLVSDRAAPGLEPGD
                     HGSTFGGNPVVCAAANAVVEAIDDELLAGVTARGDELARGLEAFPAVQEVRGSGLLLG
                     ARLDRPAADVVDGCRERGLLVLSAGSDVLRLTPPLVIGEPEVAEALGVIGDVLAA"
     gene            complement(3767..4435)
                     /locus_tag="AC150V_02685"
     CDS             complement(3767..4435)
                     /locus_tag="AC150V_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR3761858.1"
                     /translation="MSRIVLKVGGRVAAESARLAPELSAGGAEVVVVHGAGPQISAEM
                     ERRGLVPGFVRGRRVTTPDVLAVVRESFIAVNTAVCEAIGERAKPLIGDEIGLVAEQI
                     EELGLVGEPVPCAPPAVLEALRAGRIPVVAPLATGPLNVNADEAASALAVGLAADRIL
                     FVSDVPGVLLNGGVVGSIAADEADRLLGDGVFEGGIVPKLVAAVHAARGGVRAEIGET
                     LVVA"
     gene            complement(4432..5592)
                     /gene="argJ"
                     /locus_tag="AC150V_02690"
     CDS             complement(4432..5592)
                     /gene="argJ"
                     /locus_tag="AC150V_02690"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR3761859.1"
                     /translation="MSVTAAGGFVASGVCAGIRRSPGPDVALVRSVVPAVGAALWTAN
                     RLQAAPVLVSKRHLEAAEPQAVVVNAGVANAATGERGVQDAEATAATAAACLGLDVEQ
                     VVVLSTGVIGVPLPLERVQSGVEAATAELSADGGGAAATAILTTDSGPKQAVRSAAGF
                     TVGGMAKGAGMIHPCLATMLAVITTDYPLQSASDAQAFLRPAVAHSFNRISVDGECST
                     NDAVVLLANGSSGVARDDAAFERALGEVCRELAAAIVADGEGATIVLEIEVLGAASAG
                     EAEAIAQRIATSPLVKTAAFGRDPNWGRVLAATGSAPWNGGFAEVDPNRLSVSFNGTV
                     VFAGGTSTGEAPSLEGSACSIAVDLGLGQGRASYLASDLSYDYVRINAEYTT"
     gene            complement(5757..6749)
                     /gene="argC"
                     /locus_tag="AC150V_02695"
     CDS             complement(5757..6749)
                     /gene="argC"
                     /locus_tag="AC150V_02695"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR3761860.1"
                     /translation="MAYVSIIGASGYAGQETLDRVLTHPELELYAVGSDSLAGQDAVA
                     LDPRLLRNGGRRVPHFITNEAALASPADVTLLCLGHEEAARLEPPTKGVVVDLSGGHR
                     FSDASVYEDWYGFVHPRSGELGEWSYGLPELVAPSGRLIANPGCYATAALLALGPLKD
                     AVEPEGVVVDAKSGMTGAGRSLKPSSHAGFVLENLSPYKVGAHQHAPEIAQLLGFPVA
                     FTPHLLPVRRGLIATCYVRSTGPDLRAVLDEAYAGSDVVTLLPEGIAPELSRVQGTDA
                     AEIGIFDDRLTGRSIVICAIDNLGKGAAGQAIQNVNAAFGFDDAAGLRLTGVRV"
     gene            complement(6793..>7228)
                     /locus_tag="AC150V_02700"
     CDS             complement(6793..>7228)
                     /locus_tag="AC150V_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR3761861.1"
                     /translation="VLYGATGGELYCAGLAGERFAVRNSGATAVIEGVGDHACEYMTR
                     GTVVILGVHGRNLGAGMTGGECFLLDADERLVNEDLVALIELRAEDEERLLVLLDRHE
                     RATGSTTARELLGEPETLRSRFRLVIPRSMLLEGELEGRLSA"
BASE COUNT         1120 a         2671 c         2405 g         1032 t
ORIGIN      
        1 gccagcaggc cggccaggcg cccgatcgcc gtacctgcct ttccgcgaac gagctccgct
       61 acgtcggggt tcagcttctg cggcatcatc gaggagccgg tggctgcagc ctccgggagc
      121 cgagcgaagc cgaactcggc cgtggcccag agcacgatct cctcgccgat gcgcgccagg
      181 tgcgtgaaca gcacagctgc cgcgtagagg tagtcgagcg cgaagtcccg atccgacacc
      241 gcatcgatcg agttcctcat ctgacgggga ggcggcggca ggtcgagcgt cgagccggcg
      301 agggccccgg cgccgagggg gctttcagca gacgcgtcgg ctgcggcggc gaagcggatc
      361 cgatcgcgtt cgagcatctc gacccatgcg aggaggtggt gccccagcgt cacaggctgg
      421 ccgcgctgca ggtgcgtgta gccgggcatg agggtgtcgg cctccgcctc ggcgaacgag
      481 aggacgacga gcgccagggc gtcgatctcc tcccgcgcct cggcgcaggc gtccagcaca
      541 tagagccgga aggccgcggc aacctggtcg ttgcgcgagc gtccggcgtg gatcttccga
      601 cccacgtccc cgagcactcg ctcgatcgcc gtgtggacgt cctcgtccga agacaggtag
      661 gcggacggat cctgcgcgag gtcggcaagc ctcgtctcgg cctcctcgag ctcggcgccg
      721 gtcagcaggc ccgcagcgtg gaggcgtccg gcgtgctcga cggtggcctc gcagtcatac
      781 gggaggagct ctgcgtcgtt cgcgcgcaga aactcccaga cggccggatc gagcgagccg
      841 ccgacccgcc cggcccacag gctcatgcct tcgccttggc ccgcgcacgg gccgcggcca
      901 gctctgtttc gagcgctgcg atctggatga agccctcggc ggcgccgtgc gggaacgtct
      961 cgcccgcccc gtaggacgcg agctgctcag cgtagagcat gtgctcggag cggcgccccg
     1021 tcaccacagc cgtgttcgcg cgcagctcga ggcgaacctc cccggtgacg agctcctgcg
     1081 tcttcgcgac gaaggcgtcg atcgcctcac gggcggggtg gaaccagagg ccctcgtaga
     1141 cgagctccgt ccagcgctgc tcgagatgcc gcttcgtcct cgcctcgtcc ttcgtgagga
     1201 tcacgtcctc gagcgcagag tgcgccgcga tcagcgtcat cgccgcgggc gcttcgtaca
     1261 cctcgcggct cttgatgccg acggcgcggt tctcgatcat gtcgatccgt ccgatgccgt
     1321 actcccccgc gagcgtgttg agcctcatga tcagggcggc gagcgggagc tcctcgccgt
     1381 cgagcgtcac gggaacgccc cggtcgaagc cgacgacgat ctcgaccggc atcggggcca
     1441 tgttgggatc cttcgtgtac tcgtaggggt cacgcggcgg ggcgttccag ggatcctcga
     1501 gcacgcccgc ctcgatcgcg cgaccgaaca gattctcgtc gatcgagtag ggagactccg
     1561 ccgtctgctt gaccgggatc cccttggcga tcgcatactc gatctcctcg tcgcgcgtcc
     1621 agatgcggtc gcgcaacggc gcgatgacgc gggccgccgg atccagcgcc ttgaacgcga
     1681 gctcgaagcg gagctggtcg ttgcccttgc ccgtgcaccc gtggacgagc gcctccgcac
     1741 cggcgtccag cgcgacctcg gccgatgcct gggcgatcac gggccgggag agcgaggaga
     1801 cgagcgggta cttcccctcg tagagcgcgt tggtctggat cgccttcgcg cactggtcgt
     1861 cggcgaacgc gtcgcggcga tcgacgagca acacgtcatc cgcccccgcc gcccgccctc
     1921 gcgccatgga ctcgtcgatg tcgaagtgct gcccgacgtc gaccagcacc gcgacgacgt
     1981 ggtcgaagcc gtaccactcc tccttcagcc aggcaatgat gcaactcgtg tcgagcccgc
     2041 cggaaaacgc gactactgcc gttccgggca ctacgcagct ccttccatca gatgttccgc
     2101 cagctcgtca cgcagcgcgc tcgcggagac ccctcgtcgt gacgcgatga agatcgcgtt
     2161 gtcccccgcc agcgtaccca cgatcgacgg atgctcgccc tcgtcgatca cctgcgcgag
     2221 ggcgctcgcg tacccggccg gcgtcgtgac tacgacgagg ccaccgccgg cggcctcgac
     2281 cgtgagagcg tatcgacgca tggccgagcc gatcgcgcgc agccgatcgg cgtcgctcgc
     2341 tcccggcgcc gcataaacaa ggcgtccgct cggggccctc accttgacga ggccgagctc
     2401 accgacgtcg cgcgacacgg tggtctgtac gacctcgaag ccctctccgc gcagcaactc
     2461 cgcgagctcc gactgcgtcg ataccgcccg ctcctggacg agctcgagga tccgcgcctg
     2521 tcgctcccgt ctgttcatag tatgcaagaa gatagcaaaa tatgcgtcgc gctcactcgg
     2581 gtctccgcac cggctggagg tcaggcggcc agcacgtcgc cgatcacgcc gagcgcctcg
     2641 gcaacctccg gctcgccgat cacgagcggc ggcgtgagcc gcaggacgtc cgagccggcg
     2701 gagagcacga gcagaccgcg ctcacggcag ccgtcgacaa cgtccgcggc gggccggtcg
     2761 agtctcgcac ccaggagcaa tccgcttccg cgcacctcct gcacggccgg aaaggcctcc
     2821 agacctcgag cgagctcgtc tcctcgggcc gtcacgcctg cgagcagttc gtcgtcgatt
     2881 gcctcgacga cggcgttggc cgccgcgcag acaactgggt tcccaccgaa cgtcgaaccg
     2941 tgatcgccgg gctcgagtcc gggtgctgct cggtcggaga cgagcaacgc gccgatcgga
     3001 agcccgtttg caagcccctt cgcgagcgtc accgcgtcgg gctggagccc gagctgctca
     3061 taggcgaaga acgttcccgt gcggcccatc ccggcctgca cctcgtcgag gacgagcagc
     3121 gcgccgactt cccgggcgat ctcggccgcc gccgtcgcga agccgcgctc caacgggacg
     3181 acgccgccct cgccgaggat cggctcgagc aggatcgcag cgatgtcgcc acccggcgtc
     3241 accgccgcct cgagcgactc gacctcgttc ggccgcgaga aggcaacccc cgggacgagc
     3301 gggccgaagc cctcccactt cggcggctgg cctgtgaccg agagcgcgcc gagcgtgcgg
     3361 ccgtggaagc caccttcgat cgcgacgatg ccgggcctgc cggtggcctt gcgcgcatac
     3421 ttgagagcgg cctcgttcgc ttctgctccg gagttgcaga agaaagcccg tgcgcctccc
     3481 atccgactgg agagcgccga ggcgagcgcg gccatcggct cgctccagta caggttcgac
     3541 gagtgccaga ggcgatcgag ctgggcgtgc gccgcctcga gcggtgacgg atggagatga
     3601 ccgagcccga cgaccgctat ccccgccacg aagtccaggt acgcacggcc ggtgtcgtcc
     3661 cagacgcgac acccctcgcc acggacgatc gtgacgttcg cgcgtgcata cgtcggcagc
     3721 agccggtctt gcagctccgc aagaaccgga ctgttccctg cccgggtcac gcgaccacca
     3781 gcgtctcgcc gatctcggct cgtacgccgc ctcgcgcggc atggactgcc gccacgagct
     3841 tcgggacgat cccgccctcg aagacaccgt cgccgagcag gcggtcggcc tcgtccgccg
     3901 caatgctgcc gacgacgcct ccgttgagca gaacaccggg aacgtcggag acgaaaagaa
     3961 tccggtccgc cgccaagccc accgcgagag ccgacgccgc ctcgtccgcg ttcacgttga
     4021 gcggcccggt cgccagcggc gccacgaccg ggatccggcc ggcgcgcagc gcttcgagca
     4081 ccgcaggcgg cgcgcacggc acgggctccc ccacgaggcc gagctcctca atctgctcgg
     4141 ccacgaggcc gatctcgtcc ccgatcagag gcttcgcccg ctcgccgatt gcctcgcaga
     4201 ccgcagtgtt gaccgcgatg aacgactctc gcacgacagc gaggacgtcc ggtgtcgtca
     4261 cgcgccgacc gcgcacgaac cccgggacga ggccgcgccg ctccatctcg gcggagatct
     4321 gagggccggc gccgtggaca accacgacct ccgcaccgcc ggccgacagc tccggcgcca
     4381 gccgtgccga ctcggcggcg acccgcccgc ccactttcag cacgatgcgg ctcacgtcgt
     4441 gtactccgcg ttgatgcgca cgtagtcgta cgagaggtcg gacgccaggt acgaggcacg
     4501 tccctgcccg aggccgagat cgacggcgat cgagcaggcg gagccctcga gcgacggcgc
     4561 ctctcccgtc gacgtcccgc ccgcgaagac gacggttccg ttgaaggaca cgctcaggcg
     4621 gttcgggtcg acctcggcga agccgccgtt ccacggcgcg gatcccgtgg ctgccagaac
     4681 ccgcccccag ttcgggtcgc ggccgaaggc cgccgtcttg acgagcggcg acgtggcgat
     4741 ccgctgcgcg atcgcctccg cctcgccggc gctcgccgcg cccagaacct cgatctcgag
     4801 cacgatcgtc gcgccttcgc catcggcgac gatcgcggcg gccagctccc gacacacctc
     4861 tccgagcgcc cgctcgaatg cggcgtcgtc ccgcgcaacc ccagaagacc cgttcgcgag
     4921 cagcacgacc gcgtcgttcg tcgagcactc accgtcgacc gagatccggt tgaagctgtg
     4981 cgcgaccgcg ggccgcagga acgcctgcgc gtcggacgcc gattggagcg ggtagtccgt
     5041 cgtgatgacc gccagcatgg tcgccaggca cggatgaatc atcccggcgc ccttcgccat
     5101 cccgcccacg gtgaaccccg cagccgagcg cacggcctgc tttgggcccg aatcggtcgt
     5161 gaggatggcg gtggccgcgg cgccgccgcc gtcggcggag agctcggccg tcgcggcctc
     5221 cacaccgctc tggacacgct cgagcggcag cgggacgccg atcaccccgg tcgagagcac
     5281 gacgacctgc tcgacatcca gcccgagaca cgcggccgcc gttgcagcgg tggcctccgc
     5341 gtcctgcaca cccctctcac cggtcgcagc attcgcgacc ccggcattga ccaccacagc
     5401 ctggggctcg gcagcctcga gatggcgctt ggagacgagc acgggtgcag cctggagccg
     5461 gttcgccgtc cacagcgcgg cgccgacggc aggcaccacg gagcgaacga gcgccacgtc
     5521 ggggccgggc gatcgtcgga tccccgcgca gacgccactc gcgacgaaac cgcccgccgc
     5581 ggtgaccgac atcctagtgc tccccgccgg cgggagcccc cgcttcgcgc ggctgccgac
     5641 gtaccgcatg ccgccgttcc gggctgcccg gatgcgggat gccagtggcg gctccccggc
     5701 tcggcctgct cgcttctcgc ccgcgactca tgaccctagc ctcccgggcg aggcacctag
     5761 acccgaaccc cggtgagccg gagccctgcc gcgtcgtcga agccgaatgc agcgttcacg
     5821 ttctggatcg cctggccggc cgcccccttg cccagattgt cgatcgcgca gatgacgatg
     5881 gagcgtccgg tgagccggtc gtcgaagatg ccgatctccg ctgcgtcggt gccctggaca
     5941 cgcgacagct ccggcgcgat gccctctggg aggagcgtca ccacgtcgct gcccgcgtag
     6001 gcttcgtcga gcaccgcgcg caagtcggga ccagtcgagc gcacgtagca ggtggcgatc
     6061 agcccacgcc gcaccggcag caggtgcggt gtgaacgcga ccgggaagcc gagaagctga
     6121 gcgatctccg gcgcgtgctg gtgggcgccg accttgtagg gtgagagatt ctcgaggacg
     6181 aagccggcat gcgagctcgg cttgagcgag cggccggcac cggtcatgcc cgacttcgcg
     6241 tcgaccacga ctccttcggg ctcgacggcg tctttgagcg gcccgagcgc cagcagcgct
     6301 gcggtcgcgt agcaccccgg gttggcaatc agtcggccgc tgggtgcgac cagctcgggc
     6361 agcccgtagc tccactcgcc gagctcgccg gaacgggggt gcacgaagcc gtaccagtcc
     6421 tcgtagacgc tcgcgtcgga gaagcggtgg ccgccggaca ggtcgacgac cacacccttc
     6481 gtcggcggct cgagccgcgc ggcttcttca tgcccgagac agagcagcgt gacgtcggcg
     6541 ggagatgcca gcgccgcctc gttggttatg aagtgcggca cgcgtcggcc cccattccgc
     6601 agcaggcggg gatcgagtgc gaccgcatcc tggccggcga gcgagtcgga gccgaccgca
     6661 tagagctcga gctcgggatg cgtcaggacg cgatcgagcg tctcctgccc ggcgtagccg
     6721 gacgcaccga tgatgctgac atacgccacc ttatgcatag tatgcagaaa ctttgcctcg
     6781 tatgcgtagc acctaggctg agagccgccc ctcgagctcg ccttctagca gcatcgaccg
     6841 cgggatcacc agcctgaagc ggctccgcag agtctccggc tcgccgagca actcgcgcgc
     6901 ggtcgtcgag ccggtcgcgc gctcgtgtct gtcgaggagc acgagcagcc gctcctcgtc
     6961 ctcggcgcga agctcgatca acgcgacgag gtcctcgttg acgagacgct cgtccgcgtc
     7021 gagcaggaag cactcaccgc cggtcatccc cgccccgagg ttccgcccgt gcacgcccag
     7081 aatcaccacc gttcctcgcg tcatgtactc gcacgcgtga tcgccgacac cctcgatcac
     7141 ggcagttgcg cccgagttcc tgacggcaaa ccgctcgccg gcgagaccgg cgcagtagag
     7201 ctcgcccccc gtggctccgt agagaacc
//
LOCUS       DBNGNK010000099         8328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_232632,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000099 DBNGNK010000000
VERSION     DBNGNK010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_232632"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="dinB"
                     /locus_tag="AC150V_02705"
     CDS             complement(<1..999)
                     /gene="dinB"
                     /locus_tag="AC150V_02705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR3761862.1"
                     /translation="MFVPEGTILHADIDAFFAAVEQRDDPRLRGRPVIVGGGVVLAAS
                     YEAKAFGVRTAMGGREARRLCPQAIVVKPRMSAYSDASRAVFRVFDDMSPLVEGMSID
                     EAFLDVRGLERISGRPAEIAMRLRQRVREQVGLPVTVGVARTKFLAKVASGVAKPDGL
                     LVVPPDRELAFLHPLGVERLWGVGSVTAQKLRRRRIMTVGEVAALPEAVLVSLLGRAA
                     GRRVHALAHNSDPRPVQVGRRRGSIGSQRALGRRPRSLEEVDAVVLGLVDRVTRRMRV
                     ARRVGRTVVLRLRFADYSRATRSHTLPQATAQTHAVLATARGLLAAARPLIERRGLT"
     gene            complement(1056..1568)
                     /locus_tag="AC150V_02710"
     CDS             complement(1056..1568)
                     /locus_tag="AC150V_02710"
                     /inference="COORDINATES: protein motif:HMM:NF028004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3761863.1"
                     /translation="MPGRLSLAVVVLIAVLALAGCGGDGGTDDSAAETATETTATGLP
                     GIPAEFSGYRDWTKLNADPIPPRDSDPHLGTKDVYASVTAEDGVYADGAIVVKEAVRP
                     DADFVGLIATMRKEAGANPEHNDWVFVEWSRDAADAEFTELAAGGVCTSCHVGARETD
                     YVFTADESGG"
     gene            complement(1578..1997)
                     /locus_tag="AC150V_02715"
     CDS             complement(1578..1997)
                     /locus_tag="AC150V_02715"
                     /inference="COORDINATES: protein motif:HMM:NF046924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiG family protein"
                     /protein_id="HIR3761864.1"
                     /translation="MEWSRDDSWPDIDRLSDAERRRLRRRLEIEERTISARRRRLHEK
                     IDMLRAELVRRIRAGEIDIATGPWSDRACRVFAGTGRLPKESPALRIDPRALSATRLR
                     RLLADLEREEDDVSLRRRVLHFRIDLLGWIDRLGSID"
     gene            2090..2413
                     /locus_tag="AC150V_02720"
     CDS             2090..2413
                     /locus_tag="AC150V_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761865.1"
                     /translation="MAEVTPARRGIESPSGDVDELGDVLRRVNQIRVRQGADPIYELP
                     TAQPAMTPGSTCVLQEAFADIGVASVDYHNLVGRGLVIEHGLGWFIRRFDDGAYPQLY
                     SRGRG"
     gene            complement(2418..3617)
                     /gene="serS"
                     /locus_tag="AC150V_02725"
     CDS             complement(2418..3617)
                     /gene="serS"
                     /locus_tag="AC150V_02725"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR3761866.1"
                     /translation="MIDLKAARAEPETMRAALSRKGAAETFDALLAADERWRALVPRV
                     DELRSRTKLKGKPTPEQLEELKQVKGELKIAEDDLAAAEAERDALLRAVPNPPDPTAP
                     DGDSEDDAVELRKVGEPPALVDPLEATEIGRFELERAARLSGSRFGFIVGDSALVALA
                     LYRLALDHVGAAGFTPIIPPVLVREEAMVGTGFFPTEKSNIYELEADELYLTGTSEVS
                     LAGMHMGEILEDDALPIRYTGFSTCFRREAGAAGKDTRGMFRVHQFNKVEMFVFTRPE
                     ESADEHERMLELEEGLMKSLGLPYRVVNVAAGDLGASATKKYDIEAWFPSQGRYREVT
                     SCSNTTDYQARRLGIRYRAGSGLEPPHTLNGTVVTDRALLALLENLQGDVPDVLSQYG
                     APGSVVR"
     gene            complement(3614..4441)
                     /locus_tag="AC150V_02730"
     CDS             complement(3614..4441)
                     /locus_tag="AC150V_02730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR3761867.1"
                     /translation="MNLPPGFPVRVDAIACDLDRTLIGEDTELRPRTLAALAAARRAG
                     IHVIVATGRMFRSVRPYLQLAGLTDPVICYQGAAVVDPTSETFLLHRPIELDLAREAI
                     VQLLDAGYEPNCYVGDELYVARETSYSRAYADFQHLPVTEVGDLLTWLDTAPTKLVAV
                     GEPSTLPSLRERLAPVFDGRLFVTTSLPHLLELGSPGVSKGSGLTFVAERLGFEPEAT
                     VAFGDGENDLELLEWAGFGVAVGNAHPTLRALADWMCPGPDEDGVARVIEAFVDSAS"
     gene            complement(4438..4851)
                     /locus_tag="AC150V_02735"
     CDS             complement(4438..4851)
                     /locus_tag="AC150V_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3761868.1"
                     /translation="MHRLIAFLSLAVVALALVLAGCGGEETATPTPDTVVGTVPEETT
                     GGGDEDLPALSLTGDAAAGAGIYDANGCGACHVLAAAGSNGNVGPNLDQTQPSYELVV
                     ERVTNGQGAMPAFGDSLSPQEIADVSQFVAEPAGS"
     gene            complement(4937..5548)
                     /locus_tag="AC150V_02740"
     CDS             complement(4937..5548)
                     /locus_tag="AC150V_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR3761869.1"
                     /translation="MPKYVLESAEVVREAARFWWLYLLTGILWIILSLIIFRFDYTSV
                     AAISILFGIVAIAFGINEFFGLGTMTTGWKIVRVVIGIIFVVVGVVALINPYDTFATL
                     AALIGFVLVVKGIFDIIVSFVTKDDIPIWWLQLIVGIVEVLLGFWASGGFERSAFLLV
                     VYVAAMALARGITEIIVAFKLHGLHEDFGGYDRGEGPPPAPAT"
     gene            5678..6346
                     /locus_tag="AC150V_02745"
     CDS             5678..6346
                     /locus_tag="AC150V_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3761870.1"
                     /translation="MIVERSLHPDWLSNAYLVADDPGGSAVFVDSGAPLEPLLDAVER
                     HGLEPTHLLVTHRHGDHIAGNDELVERFGVEVTSGTVETGGLHVEVLETPGHSDDGVT
                     FVVNGELCFTGDTLFRDAVGGGPATELRRSVMDVLMVLPHGLRVLPGHTDETTIGREW
                     EANPFIRYWRGEASEDGRRCRLNGEDGTIVVWSPDYDGKGKLLVRLDSGGEAIVGASR
                     AEIV"
     gene            complement(6390..7340)
                     /locus_tag="AC150V_02750"
     CDS             complement(6390..7340)
                     /locus_tag="AC150V_02750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3761871.1"
                     /translation="MKALIFHGPGDIRIEEVPDPAPRQEGDVLVQIEVALTDGTDLKA
                     FRRGHPVLLGEPPAPFGHEFCGIDVATGRRVVAANSAPCRSCGPCSRGQETLCVNLLP
                     LLNGAYAELLVVPARIAGVNLLPVPPDMPGELAALTEPLACCLHGVEAAGVDADDRVA
                     IVGLGPIGLMLCACVADAGASPLAVSNRPERGELARDFGAQPDEGAAADIVIEAAGTE
                     AAWRRALELVRPGGTVVAFGGLARESRVEVDAYRIHYEEVTLRGSFHHTPRNVRAALA
                     FLASGAYPFERLVTHRVGLGELPALFAAPPVGYLKAAVYP"
     gene            complement(7337..8182)
                     /locus_tag="AC150V_02755"
     CDS             complement(7337..8182)
                     /locus_tag="AC150V_02755"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3761872.1"
                     /translation="MRALVLGPSGEPELADIEEPTGEGEPVRVLACGLCGSDVEKFGQ
                     AAPGTVLGHEVVAETAAGDRVALVHHVGCGECERCRAGHESTCELFAQPTIVPGGFAE
                     RALARGWVTLPDHVDDAYGTYVEPLACVLRGAERLQRGRALVVGHGFVGRLFSAVLRD
                     RGDEVFTVDADPARSGPPPDGPVDAVVLCAPAGTAAALDALIPGGTLLVFAAAGELPS
                     DAVYRRELTVTGSRSATPRHMSEAADLVPRLDLPEPLVLPLARFPEGVDLYRSRSVLK
                     VVFTP"
BASE COUNT         1302 a         2872 c         2941 g         1213 t
ORIGIN      
        1 tgtgaggccc cgtcgctcga tcagaggcct cgcggcggcc agcagcccgc gcgcggtcgc
       61 caggaccgcg tgggtctggg cggtcgcttg gggcagcgtg tgcgagcggg tcgcgcgcga
      121 atagtcggca aagcgaagcc ggagcaccac ggtgcggccg acgcgacggg ccacccgcat
      181 ccggcgcgtc acgcggtcga cgagtcccag gacgacggca tcgacctcct cgagcgatcg
      241 cggcctgcgg ccgagggcgc gctgtgagcc gatcgagcct ctgcggcgac cgacctgcac
      301 cggccgggga tcgctgttgt gggccagcgc atgcacgcgg cggccggcgg ctcggccgag
      361 cagcgacacg agcacggcct cgggaagcgc ggcgacctcg ccgacggtca tgatccgacg
      421 gcggcgcagc ttttgggcgg tcacactgcc gacgccccag aggcgctcga ctccaagcgg
      481 gtgcaggaac gccagctcgc ggtccggcgg aaccacgagg agaccgtcgg gcttggcgac
      541 accactcgcc actttggcga ggaacttcgt tcgagccact ccgaccgtca ccggcagacc
      601 cacctgctcg cgcaccctct gccgcagccg catggcgatc tcggccgggc gccccgagat
      661 ccgctccagc ccgcggacgt ctaggaacgc ctcgtcgatc gacatcccct cgaccaacgg
      721 cgacatgtcg tcgaagaccc ggaagaccgc cctgctcgcg tcggagtacg cagacatgcg
      781 cggcttcacg acaatcgcct gtgggcacag ccggcgggcc tcgcggccgc ccatggcagt
      841 gcggacgccg aaggccttgg cctcgtagct cgcggcgaga acgacgcccc cgcccacgat
      901 cacggggcgg ccgcgcaggc gcgggtcgtc ccgttgctcg accgccgcga agaacgcgtc
      961 gatgtccgcg tgcaagatcg tcccctctgg cacgaacata tgttcgcata gttcgggacg
     1021 tgccgccaag ggactgcggc cgcgcaggtc tcggcctagc cgccgctctc gtcggcggtg
     1081 aagacgtagt ccgtctcgcg tgcgccgacg tggcaggagg tgcatacgcc gcccgcagcg
     1141 agctcggtga actcggcgtc ggcggcgtcg cgtgaccact cgacgaagac ccagtcgttg
     1201 tgctccgggt ttgcgccggc ctccttgcgc atcgtcgcga tcagcccgac gaagtcggcg
     1261 tcgggccgca ccgcctcctt gacgacgatg gcgccgtccg cgtaaacgcc gtcttcggcg
     1321 gtcacgctgg cgtagacgtc tttcgtcccg aggtgcgggt cgctgtcgcg cggtggaatc
     1381 ggatcggcgt tgagcttcgt ccagtccctg tacccggaga actctgcggg gatgccggga
     1441 agccctgtgg cggtcgtctc cgtagccgtc tctgccgccg agtcgtcggt tccgccgtca
     1501 ccgccgcacc ccgcgagagc aagaacggcg atcagtacga cgacggcaag ggaaagccgg
     1561 ccgggcatgc agccagccta gtcgatcgag ccgaggcggt cgatccaccc cagcagatcg
     1621 atgcggaagt ggagaacacg gcgtcgcaac gagacgtcgt cctcctcgcg ttctaggtcg
     1681 gccaggaggc ggcgcagccg cgttgcggaa agggcgcgcg ggtcgattcg gagcgctggg
     1741 ctctccttgg gcagccggcc ggttcctgcg aagacgcggc aggcacggtc gctccagggc
     1801 ccggttgcga tgtcgatctc cccggcccgg atccggcgaa cgagctccgc ccggagcatg
     1861 tcgatcttct cgtgcagtcg ccgccggcgg gccgagatcg tacgttcctc gatttcgagc
     1921 cgccgtcgca gccgccggcg ctcggcgtcg ctcagccgat cgatgtccgg ccacgaatcg
     1981 tcccgagacc actccatcca gcctccactg ccccgttgtc gctcaacatc ggccttgcgg
     2041 ggtcgctact tgagcgcgcg tacggcgacc gtgaagctac gcttgcgtcg tggcggaggt
     2101 aacgcctgca cgcaggggca tcgagtcgcc gagcggcgac gtcgacgagc tcggcgacgt
     2161 cctgcgccgg gtcaaccaga tccgcgtgcg acaaggggcg gatccgatct acgagctgcc
     2221 gaccgcccag cctgctatga ccccgggctc gacgtgcgtg ctgcaggagg cattcgcgga
     2281 catcggcgtc gccagcgtcg actaccacaa cctggtcggc agggggctcg tgatcgagca
     2341 cgggctcggc tggttcatcc ggcgcttcga cgacggggcc tacccccagc tctattcgcg
     2401 cggccgcggc tgatcgatca acgcacgacg gatccgggcg cgccgtactg cgacaggacg
     2461 tccgggacgt cgccctgcaa gttctcgagc aacgcgagca gcgcgcggtc ggtgaccacg
     2521 gttccgttga gcgtgtgggg cggctcgaga ccgctcccgg cgcggtagcg gatgccgagg
     2581 cgccgtgcct gatagtcggt ggtgttcgag cacgacgtca cctcgcggta gcgcccctgg
     2641 ctcgggaacc aggcctcgat gtcgtacttc ttcgtcgccg acgcgccgag atcgcctgcg
     2701 gcgacgttga cgacacggta ggggagcccc agcgacttca tcaacccctc ctcgagctcg
     2761 agcatgcgct cgtgctcgtc cgccgactcc tcggggcggg tgaagacgaa catctccacc
     2821 ttgttgaact ggtggacgcg gaacatcccg cgtgtgtcct tcccggccgc accggcctcc
     2881 cggcggaagc aggtcgagaa gcccgtgtag cggatcggga gcgcatcgtc ttcgaggatt
     2941 tcgcccatgt gcatgccggc gagcgagacc tcggacgtcc ccgtcaggta gagctcgtct
     3001 gcctcgagct cgtagatgtt cgacttttcg gtcgggaaga agcccgtgcc gaccatcgcc
     3061 tcctcgcgga ccagcaccgg agggatgatg ggtgtgaagc cggccgcgcc gacgtggtcg
     3121 agggcaaggc ggtacagcgc gagcgcgacc agggcggagt cgccgacgat gaagccgaac
     3181 cgcgatccgg agaggcgggc cgcgcgttcg agctcgaagc gaccgatttc cgtggcctcc
     3241 agcggatcga cgagcgcagg cggctcgccc accttgcgca gctcgacggc gtcgtcctcg
     3301 ctgtccccgt cgggcgctgt cgggtccggc ggattcggaa ccgcgcgcag aagcgcgtcg
     3361 cgctcggcct ccgccgcagc aaggtcgtcc tctgcgatct tgagctcccc tttgacctgc
     3421 ttcagctctt ccagctgctc gggcgtcggc ttgcccttga gcttggtgcg actgcgcagc
     3481 tcgtcgactc tcggcacgag tgcgcgccaa cgctcgtcgg ccgccaggag agcgtcgaag
     3541 gtctcggccg cgcccttccg ggagagcgcc gcgcgcatag tctcgggctc agcccgcgct
     3601 gccttgaggt cgatcacgag gcggagtcta cgaaggcctc gatcacccgt gcgactccgt
     3661 cctcgtcggg ccccgggcac atccagtccg caagggcccg gagagtcggg tgtgcgttcc
     3721 cgaccgctac gccgaaaccg gcccattcga gcagctcgag atcgttctct ccgtctccga
     3781 acgcgacggt cgcttcgggt tcgaagccta ggcgctccgc cacgaaggtc aggccgcttc
     3841 ccttcgagac gcccgggctt ccgagctcga gcaggtgtgg cagcgaggtg gtcacgaaga
     3901 gccgcccgtc gaagaccggc gcaaggcgct ctcggagtga gggaagcgtg ctcggctcgc
     3961 cgaccgcgac aagcttcgtg ggcgccgtgt cgagccacgt caacagatcg ccgacctcgg
     4021 tgaccggtag gtgctggaag tcggcgtagg cgcgtgaata ggaggtctcc ctggcgacgt
     4081 acagctcgtc gccgacgtag cagttcggct catagccggc gtcgagcagc tgcacgatcg
     4141 cctcccgtgc gaggtcgagc tcgatgggac gatggaggag gaaggtctcg ctggtcgggt
     4201 cgacgacggc ggcgccctgg taacagatca ccgggtcggt caacccggcc agctggaggt
     4261 agggacggac ggagcggaac atcctgcccg tggcgacgat gacgtggatg ccggcccggc
     4321 gcgcggcagc gagcgctgca agggtgcggg gccttagctc agtgtcctcg ccgatcagcg
     4381 ttcgatcgag atcgcaggcg atcgcgtcga cgcggacggg gaagcccggc ggaaggttca
     4441 gctgcccgct ggctcggcga cgaactgcga aacgtcggca atctcctgcg ggctgaggga
     4501 gtcgccgaag gccggcatcg ccccctgacc gttcgtcacg cgctcgacga ccagctcgta
     4561 cgagggctgg gtctggtcga ggttcggacc gacgttgccg ttcgagccgg cggcggcgag
     4621 cacgtggcag gcgccgcagc cgttcgcgtc gtagatcccc gcccccgctg ccgcgtcgcc
     4681 cgtcaaagag agcgccggca gatcctcgtc accgcccccg gtcgtctcct ccggcacggt
     4741 gccgacgacc gtgtccggcg tcggcgtagc agtctcttcc ccgccgcagc cggcgagcac
     4801 gagcgcgagt gccacaacgg cgagagagag aaacgcgatg aggcggtgca tcggcgccag
     4861 tgtacccggg cggctcggtc gcacgcgcga agaacagcgg cgtggggccg ccggtgctgc
     4921 cccacgccta tgtctgctag gtggcaggtg cggggggcgg gccctcgccc cggtcgtatc
     4981 ccccgaagtc ctcgtgcagg ccgtgcagct tgaaggccac gatgatctcc gtgattcccc
     5041 gcgccagcgc catcgccgcg acgtagacga cgaggaggaa ggcggagcgc tcgaagccac
     5101 ccgacgccca gaagccgagc agcacctcca cgatcccgac gatgagctgg agccaccaga
     5161 tcgggatgtc gtccttcgtc acgaaggaga cgatgatgtc gaaaatgccc ttgacgacga
     5221 ggacgaagcc gatcagagcg gcgagcgtcg caaaggtgtc gtacggattg atcagcgcga
     5281 cgaccccgac gacgacgaag atgatgccga tgacgactcg aacgatcttc catcccgtcg
     5341 tcatcgtccc gagcccgaag aactcgttga ttccgaatgc gatggcgacg atcccgaaca
     5401 agatcgagat cgctgcgacg ctcgtgtagt cgaagcggaa gatgatcagc gacaggatga
     5461 tccagaggat tccggtcagc aggtagagcc accagaatct cgcggcctcc ctgacgacct
     5521 ccgccgattc gaggacgtac ttgggcatgg gctcaggcct cctagtgttc ggcgcgcagt
     5581 agctggctgg ctctgattct cctacgtgcg tcgggggttt gcaggccgca ccagccggat
     5641 agacgcattc ttccgccgct gcgatagcct gctggccatg atcgtcgagc gctcgctgca
     5701 ccccgactgg ctctccaacg cgtacctcgt cgccgacgat ccgggcggca gcgcggtctt
     5761 cgtcgacagc ggcgcgccgc tcgagccgct gctcgatgcc gtcgagcgtc acgggctcga
     5821 gccgacccat cttctcgtca cgcaccggca cggcgaccac atcgccggca acgacgagct
     5881 ggtcgagcgc ttcggggtgg aggtgacctc gggaacagtc gagacgggcg gactgcacgt
     5941 ggaggtgctc gagacgccgg ggcactcgga cgacggcgtg acgttcgtcg tcaacggcga
     6001 gctgtgcttc acgggagaca cgctcttccg tgacgcggtc ggcggcgggc ccgcgaccga
     6061 gttgcgccgt tcggtgatgg acgtgctcat ggtgctgccg cacgggctcc gcgtcctgcc
     6121 cggccacacc gacgagacga cgatcggtcg tgagtgggag gcgaaccctt tcatccgcta
     6181 ctggcgcggc gaagcctccg aggacgggcg gcgctgccgc ctgaacgggg aggacgggac
     6241 gatcgtcgtc tggtcgcccg actacgacgg caaggggaag ctgctcgtgc ggctcgactc
     6301 aggcggcgag gcgatcgtcg gcgcgtcgcg ggccgagatc gtctagcgca ccggccgggc
     6361 acggcgcgcg caacggcggc gacgctgagc taggggtaga cggctgcctt gaggtagccg
     6421 accggcggcg ccgcgaacaa tgcgggaagc tcgccgagac ccacccggtg tgtcacgagg
     6481 cgctcgaagg ggtacgcgcc gctggcgagg aacgcgaggg ccgctctgac gttcctgggc
     6541 gtgtggtgga aggaaccgcg gagagtcacc tcctcgtagt ggatgcggta cgcgtcgacc
     6601 tcgacgcggc tctcgcgtgc gagccctccg aacgcgacga ccgtcccgcc cggccggacg
     6661 agctccagcg cccgccgcca ggccgcctcg gtgccggccg cctcgatcac gatgtccgcc
     6721 gccgcgccct cgtcgggctg cgcgccgaag tcgcgcgcca gctcaccgcg ttccggtcga
     6781 ttcgacacgg cgagcgggga cgcgcccgcg tcggcgacgc aggcgcacag catcagcccg
     6841 atcgggccga gtcccacgat cgcgacccgg tcgtccgcat ctacaccggc ggcctcgacg
     6901 ccgtgcaggc agcacgccag gggctcggtc agcgcagcga gctcgccggg catgtcgggg
     6961 ggcacgggca agaggttcac tccggcgatc cgggcgggca ccacgagaag ctccgcatac
     7021 gcgccgttca ggagcggcaa caggttcacg cacaacgtct cctgtccgcg ggagcagggg
     7081 ccgcacgatc ggcagggtgc cgagttcgca gccacgacgc ggcggccggt tgcaacgtcg
     7141 atcccgcaga actcgtgccc gaacggggcg ggtggctccc cgagcagcac aggatgcccg
     7201 cgtcgaaaag ccttcaggtc ggtcccgtcg gtcagcgcca cctcgatctg tacgaggacg
     7261 tcgccctcct gacgtggtgc ggggtccggc acctcctcga tccggatgtc gccggggccg
     7321 tggaagatca aggccttcac ggtgtgaaaa cgaccttcag cacgctgcgg ctccgataca
     7381 ggtcgactcc ttccggaaag cgggcgagcg gcaggacgag gggctcagga agatcgagcc
     7441 gcggtacgag gtcggcggcc tcggacatgt gccgcggcgt cgcggagcgg gagccggtca
     7501 ccgtcagctc tcggcggtac acggcgtcgc tcggaagctc gcctgcggct gcgaagacca
     7561 gcagcgtccc gccggggatg agtgcgtcga gggccgcggc ggtcccggcc ggggcgcaga
     7621 ggacgacggc gtcgaccggg ccgtccggcg gcggccccga ccgcgccggg tcggcgtcca
     7681 cggtgaagac ctcgtctccg cggtcacgga gaacggccga aaagagacgt cctacgaatc
     7741 cgtgcccgac cacgagggca cgcccacgct ggagccgctc ggcgccgcgc aacacacacg
     7801 cgagcggctc gacgtacgtg ccgtacgcgt catcgacgtg gtccggcagg gtgacccagc
     7861 cgcgcgccag cgcccgctcg gcgaagccgc ccgggacaat cgtgggttgt gcgaagagct
     7921 cgcaagtcga ctcgtgaccg gcgcggcagc gctcgcactc tccgcacccc acgtgatgga
     7981 cgagcgcgac acggtcgcct gcggccgtct cggcgacgac ctcgtgtccg agcacagtgc
     8041 cgggagccgc ctggccgaac ttctcgacgt ccgagccgca gagcccgcag gcgagaacgc
     8101 gcaccggctc gccctcgccg gtcggctctt cgatgtccgc gagctcgggc tccccggacg
     8161 gccctaggac gagcgctcgc acatgcgtat cgtaggcccg tcatcgagcg ggaggcattg
     8221 ggccgcggct gactcgccgt gaccgccgct agaatccgcg ccgcgcgggg ctatagctca
     8281 gttgggagag cgcctggatc gcacccagga ggtcgccggt tcgagtcc
//
LOCUS       DBNGNK010000100         5940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_948696,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000100 DBNGNK010000000
VERSION     DBNGNK010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5940
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_948696"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC150V_02760"
     CDS             <1..183
                     /locus_tag="AC150V_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3761873.1"
                     /translation="FENETFTKAATGRIPLGRILQPDEIAGAIVFLASPLAAAVTGVV
                     LPVDGGWTAGEPALPW"
     gene            complement(195..581)
                     /locus_tag="AC150V_02765"
     CDS             complement(195..581)
                     /locus_tag="AC150V_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013845216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3761874.1"
                     /translation="MDAEQIRLDPDPYEPFRLSQGFRVGDLLLISGQAAIDENGQIVG
                     EGDFDAQAEQTFRNLKRVLEAGGSGLDRVVKVTIFLTDMGNFPKIVELRGKWFSEPWP
                     ADTIVEINSLALPELEIEIEAIALVG"
     gene            complement(581..1537)
                     /locus_tag="AC150V_02770"
     CDS             complement(581..1537)
                     /locus_tag="AC150V_02770"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3761875.1"
                     /translation="MADELRPEPLLDQDGVKVSKSPWGPDDEIGRLNWITPETTAAVL
                     NHLDGRHVFDLNVEYFIGMPSWVAAGDPKYEIWMTHTPQGSINDNLSGVGSHVHEKYS
                     YCGDSIHMYTHCGTHIDTLNHLGFYGKMWNGWTADADLGSRIWNKGGLENYPPVIARG
                     VLLDVAGTHGVDQLDDSYAITPKDLQDTVAKQGVELRKGDVVLVRTGRMNAWPDFDGY
                     LVNTPGIGLDAAKWLCEETGAMCIAGDTIGLEVLPSEEPDVFLPVHAYMFATAGAQII
                     EVVDMNEIAAEKQYEFAFLGTPLKLRGATGAPMPSYAVPLKS"
     gene            complement(1550..2392)
                     /locus_tag="AC150V_02775"
     CDS             complement(1550..2392)
                     /locus_tag="AC150V_02775"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR3761876.1"
                     /translation="MLVGVAQIEPRFGETERNLELCLGQLEQAAAEGCELLVLPECAL
                     TGYVCSTPDEAADVALNVPGDEVAALEAASARVGAHVVCGLLERDGDRVRNCAVLVGP
                     DGLVGRYWKTHLPYLGVDRFVTPGGGPFEPFDTPLGKIGLQICYDLRFPEVTRALALA
                     GTEIVAHPTNWPVEARAVAEIITRARALENRVFLLTSNRVGVERTAEFCGWSQIVDPT
                     GTRLAEADATSEALLVAEIDVADARVKDIIPEPGEYEMRFFDHRRPELYGSLQTQPQT
                     VNTR"
     gene            complement(2436..3314)
                     /locus_tag="AC150V_02780"
     CDS             complement(2436..3314)
                     /locus_tag="AC150V_02780"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761877.1"
                     /translation="MTRVAFIGLGTMGTPMARNLVAAGHELVGCDVDPARVEALGIPG
                     AATPAEAASWAEVTITSLPAPEIVRSVVLGEDGVLARAEPGSLYVDMSTGPPALAREL
                     ADALVGRKIDCLDAPVSGGPHGAAPATLTVMVGGEQGAFERARPLLEQLGSLVVHVGG
                     HGAGQAAKLCNNLVAGVTMAALAEACAIAEREGIEPGTLYELLGASTGDSRVLRNRYP
                     LAGADEAHPASHEWTPLFALDLIAKDLHLVEELAQQHGVDPAMTVAALAEYARAQADG
                     HGALDYSAVYLSKRPV"
     gene            complement(3319..3888)
                     /locus_tag="AC150V_02785"
     CDS             complement(3319..3888)
                     /locus_tag="AC150V_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HIR3761878.1"
                     /translation="MANDGIRKVVTLVEDVHREAGEPVDPPTRMVAAGAVIENPFAGQ
                     YVDDLEPLIDRYCELLGEQLTARALDLLDAPVEGFGKGALVGLDGDIEHGSAIIHNLR
                     FGNFVRDGSRGTSLLPAAEKRGAGGATLDIAIKHKDDHSVRSHHQTFEVRIPDAPRAD
                     EIVIWVALAASGRPNARLRAFGSELETTT"
     gene            3910..4497
                     /locus_tag="AC150V_02790"
     CDS             3910..4497
                     /locus_tag="AC150V_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HIR3761879.1"
                     /translation="MESIRFYYDVVCPYSYMESHAVEAAEDAGRVEVEWLPFELRPAP
                     RPLLEVRGDHLRTDWMRNVYRRALERGIEIHLPRYQPRSTLIVSACLFAGEQGRLRAF
                     KHALYEAFFCEGEDIATDAEIARAAERAGLDPADALVAAYSDELHGRIRSIRAEAEEA
                     GVRGVPTLLTADGRTHWGMGGVERLLAGESLVPRT"
     gene            4555..4986
                     /locus_tag="AC150V_02795"
     CDS             4555..4986
                     /locus_tag="AC150V_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761880.1"
                     /translation="MTPFRFALVAVFLAAAAVPVGIGSLSADERRTEAAEEVEARRVV
                     RELFTALNARRYDETCALLADGYFARRAAERRHCDIGLRVGFLWSQEIRWRITEVRAE
                     GNLVFVSTLADGAPGFLVLVRAHGGLRVLDVVELGDRRERL"
     gene            5011..>5940
                     /locus_tag="AC150V_02800"
     CDS             5011..>5940
                     /locus_tag="AC150V_02800"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR3761881.1"
                     /translation="MSTQTDLRSGDELLGFRVERVVGRGGMGVVYLAEQVVLDRLVAL
                     KLLAPELAANSEFRERFLRESRLAASLDHPNIVPVFDAGEVEGRLYIAMRYVEGRDLG
                     VLLAEEGTLGPERAVALLAPIADALDAAHAKGLVHRDVKPSNVLVDQAERTYLADFGL
                     TKESSEKGLVEQSHFAASVEYVAPEQIEGQPVDGRADQYALDCVLYKALTGATPYANG
                     STMATLWGHLDEPPPKVSERNPELPGEIDAVFERALAKAPGERFVTCTEFTVAVRDAL
                     GLERTPRLLRPRWLVASAAALALVVAAAVLGAVL"
BASE COUNT          827 a         1953 c         2166 g          994 t
ORIGIN      
        1 ttcgagaacg agacgttcac gaaggctgcg acgggtcgga tcccgctcgg ccgaatcctc
       61 cagcccgacg agatcgcggg cgcgatcgtc ttcctcgcca gcccgctcgc ggcggccgtg
      121 accggcgtcg tcctcccggt cgacggcggc tggaccgcgg gcgagcccgc tctgccctgg
      181 tagtcgcagc gctgtcagcc gacgagcgcg atcgcctcga tctcgatctc gagctcaggc
      241 aaggccagcg agttgatctc cacgatcgtg tccgccggcc acggctccga gaaccacttc
      301 ccgcgcagct cgacgatctt cgggaagttg cccatgtcgg tgaggaagat cgtcaccttg
      361 acgacccggt cgaggccgga gccgccggct tccagaactc gcttcaggtt gcggaacgtc
      421 tgctccgcct gcgcgtcgaa gtcgccctcg ccgacgatct ggccgttctc gtcgatcgcc
      481 gcctggccgg agatgaggag gaggtcgccg acccggaacc cctgcgagag cctaaacggc
      541 tcgtaggggt ccgggtcgag acggatctgc tcggcgtcca tcagctcttc aggggtacgg
      601 cgtacgacgg catcggcgca ccggttgccc cgcgcagctt gagcggcgtg ccgaggaagg
      661 cgaactcgta ctgcttctcg gctgcgatct cgttcatgtc gacgacctcg atgatctggg
      721 ccccggccgt cgcgaacatg tacgcgtgca ccgggaggaa gacgtcgggc tcctcggagg
      781 ggagcacctc caggccgatc gtgtcgccgg cgatgcacat ggcgcccgtc tcctcgcaga
      841 gccacttcgc ggcgtccagg ccgatgcccg gcgtattgac gaggtagccg tcgaagtccg
      901 gccaggcgtt catgcggccg gtgcggacga gcacgacgtc ccctttccgg agctcgacgc
      961 cttgcttggc caccgtgtcc tggaggtctt tgggcgtgat cgcataggag tcgtcgagct
     1021 ggtcgacgcc gtgcgtgccg gcgacgtcaa gcaggactcc gcgagcaatc accggcgggt
     1081 agttctcgag gccgcccttg ttccagatgc ggctgccgag gtcggcgtcg gcggtccagc
     1141 cgttccacat cttcccgtag aagccaaggt ggttgagcgt gtcgatgtgc gttccacaat
     1201 gcgtgtacat gtggatcgag tcgccgcagt agctgtactt ctcgtgcacg tggctgccga
     1261 cgccggacag gttgtcgttg atcgatccct gcggggtgtg cgtcatccag atctcgtact
     1321 tcgggtcgcc ggccgccacc cacgacggca tgccgatgaa gtactcgacg ttgaggtcga
     1381 agacgtggcg cccgtcgagg tggttcaaga ccgcggcggt ggtctccggg gtgatccagt
     1441 tcaagcgacc gatctcgtcg tccggccccc atgggctctt cgacaccttc acgccgtctt
     1501 ggtcgagcag cggttcgggt ctgagctcgt ctgccatctc cgtcgcctcc tatctcgtgt
     1561 tgactgtctg aggttgggtc tgcaacgacc cgtagagctc gggccgccgg tgatcgaaga
     1621 aacgcatctc gtactcgccg ggctccggga tgatgtcctt cacccgggcg tcggcgacgt
     1681 cgatctcggc gacgagcagc gcctcgctcg tcgcgtcggc ctcggccagt cgcgttcctg
     1741 tcggatcgac gatctggctc cagccacaga actccgcggt tcgctccacg cccaccctgt
     1801 tcgaggtcag gaggaacaca cggttctcga gcgcgcgggc cctggtgatg atctcggcca
     1861 ccgcgcgcgc ctcgacgggc cagttggtcg ggtgagcgac gatctccgtc cctgcgagcg
     1921 ccagcgctcg ggtcacctcg gggaagcgga ggtcatagca gatctgcagt ccgatcttcc
     1981 cgagcggggt gtcgaacggt tcgaagggcc cgccgccggg tgtgacgaag cggtcgacgc
     2041 ccaggtacgg caggtgggtc ttccagtagc ggccgacgag gccgtccggc cccaccagca
     2101 cggcgcagtt gcggacgcgg tcgccgtctc gttcgagcag cccgcagacg acgtgggccc
     2161 cgacccgggc ggatgccgcc tcgagtgcgg ccacctcgtc tcccggcacg ttcagagcca
     2221 cgtcagccgc ctcgtcgggc gtcgagcaga cgtagccggt cagtgcgcac tcggggagga
     2281 cgaggagctc gcagccctcg gctgccgcct gttcgagctg cccgaggcag agctcgaggt
     2341 tccgctcggt ctcaccgaac cggggttcga tctgggcaac gccgacaagc atcgagaccg
     2401 cgtacctccg agatcgcggg gtcggttcga gcctattaca ccggccgctt cgagaggtag
     2461 acagcggagt agtcgagcgc accgtgcccg tccgcctgcg cccgtgcgta ctcggccagg
     2521 gcggcgacgg tcatcgcagg gtcgaccccg tgctgctgtg ccagctcctc gaccagatgc
     2581 aggtccttgg cgatcaggtc gagcgcgaag agcggtgtcc actcgtgcga ggcggggtgc
     2641 gcctcgtcgg cgccggcgag cgggtagcgg ttgcggagga cgcgcgagtc gccggtcgag
     2701 gcaccgagca gctcgtagag cgtgccgggc tcgatcccct cgcgctcggc gatcgcgcag
     2761 gcctcggcca gcgccgccat cgtcaccccc gcgacgaggt tgttgcagag cttcgcggcc
     2821 tgaccggcgc cgtggccgcc gacgtggacg acgagcgagc cgagctgttc cagcagcggt
     2881 cgggcccgct cgaaggcgcc ttgctcgccg ccgaccatga cggtcagcgt tgcgggggcg
     2941 gcgccgtggg gaccgccgct gacgggggca tcgaggcagt cgatcttcct tccgacgagg
     3001 gcgtcggcca gctcgcgggc gagtgcgggt gggccggtcg acatgtcgac gtagagcgag
     3061 ccgggctcgg cacgcgcgag gacaccgtct tccccgagca cgaccgagcg gacgatctcg
     3121 ggggccggga gggaggtgat cgtgacctcg gcccaggacg ccgcttccgc gggcgtcgcg
     3181 gccccgggga tgccgagcgc ctcgacgcgg gccgggtcga cgtcgcagcc gacaagctcg
     3241 tgcccagcgg cgacgaggtt gcgtgccatc ggggtgccca tcgtgcccag cccgatgaac
     3301 gcgacgcgcg tcacgcggct aggtcgtcgt ctcgagctcg ctgccgaagg cccgcagtcg
     3361 ggcgttgggg cggccgctcg ctgccagcgc gacccagatc acgatctcgt cggcacgcgg
     3421 cgcgtccggg atgcgcacct cgaaggtctg gtggtgcgag cggacgctgt ggtcgtcctt
     3481 gtgcttgatg gcgatgtcga gcgtcgcgcc gcctgcgccg cgcttctcgg cggcgggcag
     3541 cagcgaggtg ccccgtgagc cgtcgcgaac gaaattgccg aagcggaggt tgtggatgat
     3601 cgccgagccg tgctcgatat cgccgtcgag cccgaccagc gcgcccttgc cgaagccctc
     3661 cacgggtgcg tccagcaagt cgagtgcccg agcggtgagc tgctcgccca agagctcgca
     3721 gtagcggtcg atcaacggct cgaggtcgtc gacgtactgc cccgcgaagg ggttctcgat
     3781 cacggcgccg gccgcgacca tgcgcgtcgg cgggtcgaca ggctcgccgg cttcgcggtg
     3841 cacgtcctcg acgagggtga cgaccttacg gatgccgtcg ttcgccacgg gtcgagcata
     3901 ctgcgttgct tggagtcgat tcgcttctac tacgacgtcg tctgccccta ctcgtacatg
     3961 gagtcccacg ccgtcgaggc ggcggaagac gccgggcgcg tcgaggtcga atggctcccg
     4021 ttcgagctgc ggccggcccc gaggccgctg ctggaggtgc ggggcgacca cctgcggacc
     4081 gactggatgc ggaacgtcta ccggcgcgcg ctggagcgcg ggatcgagat ccatctgccc
     4141 cggtatcagc cgcgctcgac gctgatcgtc tccgcctgcc tcttcgcggg cgagcagggg
     4201 aggctgcggg cgttcaagca cgcgctctac gaggcgttct tctgcgaggg cgaggacatc
     4261 gccaccgacg cggagatcgc ccgggcagcg gagcgtgcgg ggctcgaccc ggccgacgct
     4321 ctggtggctg cgtactcgga cgagctgcac gggcgcatcc gctcgattcg cgccgaggcc
     4381 gaggaagcgg gagtgcgcgg cgtgccgacg ctcctgacgg ccgacggccg cacgcactgg
     4441 ggcatgggcg gcgtcgagcg tctgctggcc ggcgagtcgc tcgttccgcg cacatagccc
     4501 ctaactcagg cgcctctcag cggcctctca gcggcaggat cgatcgtccg ccgcatgacg
     4561 ccgtttcggt tcgccctcgt ggccgtcttc cttgccgccg ccgccgtgcc ggtgggaatc
     4621 ggctccctct ccgccgatga gcgtcggacg gaggcggccg aggaggtcga ggcccggcgg
     4681 gtcgtccgcg agctcttcac tgcgctgaac gcgcggcgct acgacgagac gtgtgcgttg
     4741 ctcgcagacg ggtacttcgc ccgccgggcc gccgagcggc gtcactgcga tatcggcctc
     4801 cgggtgggct tcctgtggtc gcaggagatc cgctggcgca tcaccgaggt cagagccgag
     4861 ggaaacctgg tcttcgtcag cactctcgcc gacggtgcgc ccggttttct cgtgctcgtg
     4921 cgagcgcatg ggggcctccg ggtgctcgat gtcgtcgagc taggagatcg gcgtgaacgc
     4981 ttgtagcatc agttgcgagt cgggatgatg atgagcacgc agaccgacct tcgctcaggg
     5041 gacgagctgc tcggctttcg ggtggagcgg gtggtcggtc gtggcgggat gggggtggtg
     5101 tacctcgctg agcaggtcgt gctggatcgg ctggtggcgt tgaagctgct ggcgccggag
     5161 ctggcggcga acagcgagtt tcgggagcgg ttcctgcggg agtcgcggct ggcggcgtca
     5221 ttggatcatc cgaacatcgt ccccgtcttc gacgccggtg aggtggaggg ccggctgtat
     5281 atcgcgatgc ggtacgtgga gggtcgggat ctcggcgtgc tgctggcgga ggagggcacg
     5341 ctggggccgg agcgcgcggt tgcgctgctg gcgcctatag ccgacgcgct ggacgcagcg
     5401 catgcgaagg ggctggtgca ccgggacgtg aagccgtcga acgtgctggt cgatcaggcg
     5461 gagcggacgt acctggcgga tttcgggttg acgaaggagt cgtcggagaa gggtctggtg
     5521 gagcagagcc atttcgcggc gtcggtggag tacgtggcgc cggagcagat cgagggccag
     5581 ccggttgacg ggagggcgga tcagtacgcg ctcgactgtg tcctctacaa ggcgctgacg
     5641 ggggccaccc cgtatgcgaa cggctccacg atggcgaccc tgtgggggca cctcgacgag
     5701 ccgccaccca aggtaagcga gcgcaacccg gagcttcctg gcgagatcga cgctgtgttc
     5761 gagcgggcgc tggcgaaggc tcccggagag cgtttcgtga cgtgcaccga gttcaccgtg
     5821 gccgtccgcg atgcgctcgg gctcgaacgg acgccgcgac ttctccgtcc gcgctggctg
     5881 gtggcgagcg ccgcggcgct tgcgctcgtc gttgctgccg ccgtcctcgg cgccgtgctc
//
LOCUS       DBNGNK010000101        12701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1450299,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000101 DBNGNK010000000
VERSION     DBNGNK010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 12701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12701
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1450299"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /gene="dnaA"
                     /locus_tag="AC150V_02805"
     CDS             complement(<1..1188)
                     /gene="dnaA"
                     /locus_tag="AC150V_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR3761882.1"
                     /translation="MNTPEPSAAQLWDEVSNRLRETLNETTYATWFGAVAPGELGHDV
                     FEVVVPNDFTREWIEGHFLGLMKAAARDAIDRDVRVRLSVAESLPAASAPSVERPGHV
                     EAEAAGEGPRTNPKYTFDLFVIGSSNRFAHAAALAVAEAPAQAYNPLFIYGGTGLGKT
                     HLLQAIGQYVSEHSRKLTTRYVTSETFMNDFINSLRDKRIEGFKHRYRTYDVLLVDDI
                     QFFEGKERIQEEFFHTFNSLYEAGRQIVISSDRPPKEIATLEERLRSRFEWGLITDIQ
                     PPDLETRIAILRKKVTTDGIQVEDPEVLTFIAGRVPTNIRELEGALTRVVAFSSLTGL
                     PLTSELAEQVLRDLFPQGDPAPEVTIDLIQDTVSDRFGLSVAELCSPRRSQSVAYPRQ
                     VAMY"
     gene            1517..1651
                     /gene="rpmH"
                     /locus_tag="AC150V_02810"
     CDS             1517..1651
                     /gene="rpmH"
                     /locus_tag="AC150V_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR3761883.1"
                     /translation="MKRTYQPNVRRRKRKHGFRARMSSRAGRAVIKRRRARGRKRLSA
                     "
     gene            1661..2011
                     /gene="rnpA"
                     /locus_tag="AC150V_02815"
     CDS             1661..2011
                     /gene="rnpA"
                     /locus_tag="AC150V_02815"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR3761884.1"
                     /translation="MQRRQRLSRSRDFDAVYRQGRSVSTRYLTLHWFPREDDPDGEPR
                     LGLAVPKTVGKAVTRNQLKRRLRASWTELGERVPRGRDYVLVARPGIADAVEARDHQW
                     LLERLDEVLGKSSA"
     gene            2008..2280
                     /gene="yidD"
                     /locus_tag="AC150V_02820"
     CDS             2008..2280
                     /gene="yidD"
                     /locus_tag="AC150V_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR3761885.1"
                     /translation="MRHLGVALVYAWRYSFGLLTPTGTCKYHPSCSQYAIDAFRAEGF
                     VKGGILATWRLLRCNPWSHGGVDYVEQQRLFRRRGKESSDAAGGRV"
     gene            2280..3161
                     /locus_tag="AC150V_02825"
     CDS             2280..3161
                     /locus_tag="AC150V_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR3761886.1"
                     /translation="MLSLLLPSLLAGILSPIENILTAILEWLHSSVGLTWAWSIVALT
                     VLVRIALVPLTVRQIHSMQNLQMHAPEMKEIQQRWKHDKQRQNEELMKFYRENKINPA
                     ASCLPIVAQIPIFISLFFVLRDFESEIFPNYPASSLEWLDLITITEPTSQGLGPLLLV
                     VYVASQLSSSYLMSGTMQRMQRILLMVLPIVFIPFILRFPSGLMIYWLTTNLWTTGQG
                     LITRRLIPKAPPQEKRSSRTPAKEEEKAPAKSRSDDGDAQRASGDKDKKSKAKQGQKK
                     VVAGPPRRVKRKRGGRR"
     gene            3158..3841
                     /gene="jag"
                     /locus_tag="AC150V_02830"
     CDS             3158..3841
                     /gene="jag"
                     /locus_tag="AC150V_02830"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR3761887.1"
                     /translation="MSDLLTAEATGETVGEAKWAAVRELERQLPGLDRNAVRFEVLSE
                     GERGLLGVGYTPARVIAAIARPEAMPPPEDESAESALVRELLQHVVSGLGVVCTIHVD
                     DHDEEIQAELAGPDLGILIGKHGQTIDAIQYLANAAAQRRLGPERRRVVIDAGGYRER
                     RTATLQSSARRSAEQAAATGRPVSLEPMTAVERRIVHEYLKDDPEVETASEGTEPNRF
                     IVIFPRRST"
     gene            3858..4436
                     /locus_tag="AC150V_02835"
     CDS             3858..4436
                     /locus_tag="AC150V_02835"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR3761888.1"
                     /translation="MLDRWLAAVVSTPGLTSLRDPAEGRRVLLDDALRAVELVRALPG
                     PIVDVGSGGGTPGIPLAVTLPERSVTLLESQRRKAAFLEGWAEQLPNVRVIRGRAEEQ
                     PVDAYGVALAKALAPPPVAAELCLPLVRRGGAAILWVGPTADRERVGRVATQLAAELS
                     EPASGLLLLRKLGGTPPGFPRRPGMAAKRPLA"
     gene            4482..5237
                     /locus_tag="AC150V_02840"
     CDS             4482..5237
                     /locus_tag="AC150V_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3761889.1"
                     /translation="MPPRTYAVANQKGGVGKTTTAVNLAACLAEAGERCLLIDLDPQA
                     NATSGLGARANGSSAHDLLDGVPLGELTKPTSLANLDLVPAKGDLAAAAVELSVREGG
                     ERYLADALAGSAGAYSFVFLDCPPSFGPLTVNALAAADRVIVPVQAEYYALEGLSQLL
                     GSINLVKARLNPDLAVTGILLTMVDGRTRLAAEVEEELRRHFRGLVFTTSVPRSVRLA
                     EAPSHGLPAIAYARQSAGAQAYWKVAMELVERS"
     gene            5221..6084
                     /locus_tag="AC150V_02845"
     CDS             5221..6084
                     /locus_tag="AC150V_02845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR3761890.1"
                     /translation="MSSAPERPGAAAPRRGLGRGLEVLVGGGGTPELLHLPVESIHPS
                     PRQPRRRFEPEATAGLAASIRLQGVLQPVVVRSRPEGGYELIAGERRWRAAREADVPT
                     LPAVVREADDRETLLLGLVENVAREDLSPVEEARAYATLIDEFELSLGDVAERVGRSK
                     PAVSNRVRLLELPEEVLWMLARGELTEGHARAVLALPEDGERVRLARRIARDGMTVRA
                     AERAAREGGARRKRRAPSPVDPALAERARLALEGLTGCAVRVRRGALEVEFGDETGLA
                     ELVEALERTAA"
     gene            complement(6104..6177)
                     /locus_tag="AC150V_02850"
     tRNA            complement(6104..6177)
                     /locus_tag="AC150V_02850"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6141..6143),aa:Ile,seq:gat)
     gene            6255..6830
                     /locus_tag="AC150V_02855"
     CDS             6255..6830
                     /locus_tag="AC150V_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR3761891.1"
                     /translation="MSFDIVGKQPIVLLKTQDENKFLPIWIGHPEAAAILMKLQGSAS
                     PRPMTHDLLTSILDQLEAEIVRITVTELRENTFYAQITILQNGHEIEIDSRPSDAIAL
                     AVRADAQIFASEEVIEESGIEFEGGEEEAQSQIARVSELADLDPEEFRRFLDEVTPEQ
                     FAEGLPEGEEPEGEEPEEETGPEEREGEEDA"
     gene            complement(6827..7087)
                     /locus_tag="AC150V_02860"
     CDS             complement(6827..7087)
                     /locus_tag="AC150V_02860"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR3761892.1"
                     /translation="MSERFEELRFLIAQNDREIVAAVNERLTLVRELWQLKLEQGLDR
                     VDPERERALRDALRAENPGPLSEEGLDELVSALLALTKRELA"
     gene            complement(7084..7788)
                     /locus_tag="AC150V_02865"
     CDS             complement(7084..7788)
                     /locus_tag="AC150V_02865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR3761893.1"
                     /translation="MSVGSATELLPRGRLWGWVALVGVVALLNYATRFATEDDTDRRN
                     VLYEYPTAISGAVFYLILLTIVVFLARGLPAREVFALRRPDSWSSALVKMVVALIAVW
                     AANVVFTFVLDLRAGDEQGIVPEAWEPDRAGAFIANFIVIAFFGPVVEELTYRGFGYW
                     AVRAVYGLLAAVLVTSVMFGLSHGLVAGLHALTVFGLAAAWLRRDTDSLYPSTLMHVF
                     FNGAALLAAVTLGTEL"
     gene            7924..10125
                     /locus_tag="AC150V_02870"
     CDS             7924..10125
                     /locus_tag="AC150V_02870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR3761894.1"
                     /translation="MTPPTRGTPRSGELFRVSRRRRRRRGNRRRGRRGLVVTLAAFAV
                     VLIAVGVGTGAAVLAFGESCDLSSLKPVSIGQNSFVYAADGSLLGAIPAERNRQPVVL
                     AKVSPWMRKATIAIEDRRFYDHGGLDLEGIARAAWENVKAGELVQGGSTITQQLVRNL
                     YISNERTVERKVKEACLALKLNDSWSKRRILATYLNQVYYGNLAYGVEAAAQTYFSKS
                     AADLDLREAALLAGLTQAPTSYDPLTQEQRARVRRTQVLRAMRETGAITTPQFRATVN
                     RPLGLKPGRLYQEIREPYFFGYVRDELIAEYGAETVRSGGLQVFTTLVPRFQRLARAA
                     ISETLTEPTDPAAAIISINPRTGAIRAMTAVTPGRAQNEFNLLSQARRQPGSTFKTFV
                     LAAAVEQGINPATSYYVSAPFTYRPEATGNCDDGSWWCVKTYDSSYGGWTSVERATLR
                     SDNAVYAQLTLDVTPQRVAETARKMGVRTPLDVNGQYVPAMGLGSIAVSPLDIASGYA
                     TLAAGGVHAEPRAIRRVVLPNGKEDTDAGWGKPRRTRVLSDGVAAAVTEILADNVLYG
                     TGTRAYFGRPAAGKTGTTDRHADAWFAGYTPQLQTTVWVGYPRGEIPMENVHGIAVSG
                     GSFPAAIWRLFMERALAPVAPADFPEPLDQPEWTTFVRGPHALTYDPFYKPPEEDEEK
                     QDEGGKEQDTPGGEGADAGDDDDAGGSTGGGATSDSPGPGGATGAGVSETP"
     gene            10243..11436
                     /locus_tag="AC150V_02875"
     CDS             10243..11436
                     /locus_tag="AC150V_02875"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR3761895.1"
                     /translation="MADLLTREEALARILERARPLAVEETPAANAAGRVLAAAAVAVT
                     DLPPFPSSAMDGFALRAADAPATLSVVGRVAAGRPARRSLAAGEAMGIATGGTVPEGA
                     DAVVPIELVVDHGATVDVPEPVAPGQYVRPRGEDVRAGGQALPAGTRLGAAQLGALAA
                     AGISHVACSRRPRVAILATGTELRPPGEPLGPGEIYESNGVMLEVLFAQAGAMVERLQ
                     SVRDDERSHRAAIERGLAADVLVTSGGVSVGPHDLVRATERALGVAEIFWGVAVRPGK
                     PVSFGVRGDTLVFGLPGNPVSSLVGAELFVRPALRALEGDSQPGPHFELGTLAESVQP
                     HPRRDDLLRVRRLFENDGVRLAPIEGQGSHMIVRGAAADALALIPAGESTMPPGSPVR
                     YLRLG"
     gene            complement(11418..>12701)
                     /locus_tag="AC150V_02880"
     CDS             complement(11418..>12701)
                     /locus_tag="AC150V_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761896.1"
                     /translation="QTLVFLSTDAGEFGGLGAARFASTSRFRENVSAVVVLEALSGPG
                     EPRLELAGDGPRSPAPTLVRTAGARLGEELGREPARPGIVRQLVDLGIPFAYGDQAPF
                     LGERISALRVTAADDSGAAAGTDTTAELNAARMERLGRGAQSLLGSLDSGAELARGTS
                     AYVYAGGRVVRGWTIALVLLAAVVPFGVGVVDLLVRCRRMAIPLQPAFAGLLGRLALW
                     LWAAVVLWLAALLGALPTGSARPLPPSGPSATDWRFGSLAALSLLIVVPWLLTRRQRG
                     AWRPPSPEEELAGYGAGLAALGIVALAAAVVNTYSLIFVVPSLYAWLWLPQTGRSWAR
                     DVLYGLGFAGPLLALVSLADRFGLGLEVLLYVSGLVTVGYLSWATVVIVLAWGAVAMH
                     VGGVASGRYSLPRADAPPLLARVRAAGRSFYPRRR"
BASE COUNT         2056 a         4290 c         4499 g         1856 t
ORIGIN      
        1 gtacatggcg acttgtcggg gatacgccac ggactgagag cgccgggggc tgcagagttc
       61 agcaacactg agcccgaagc ggtcggagac cgtgtcttgg atgaggtcga tcgtcacctc
      121 gggagccgga tccccctgcg ggaacagatc gcgcaacacc tgttcggcaa gctcgctcgt
      181 caagggcagt cccgtgagtg aggagaaggc cacgacgcgc gtcagcgcgc cctccagctc
      241 gcggatgttc gtggggacgc gcccggcaat gaacgtgagg acctctgggt cctcgacctg
      301 gatgccgtcg gtggtcacct tcttgcgcag aatcgcgatc cgggtctcga gatcgggggg
      361 ctggatgtcg gtgatgaggc cccattcgaa ccgcgagcgg aggcgctctt cgagtgtcgc
      421 gatctccttg ggcggacgat cggacgagat cacgatctgt cgcccggcct cgtagagaga
      481 gttgaaggtg tggaagaact cctcctggat ccgctccttg ccttcgaaga actggatgtc
      541 gtcgacgagg aggacgtcgt aggtccggta gcgatgcttg aagccctcga tgcgcttgtc
      601 gcgcagcgag ttgatgaagt cgttcatgaa cgtctcgctg gtcacgtagc gcgtcgtcag
      661 cttccgtgag tgctcactca cgtactgccc gatcgcttgc agcagatgcg tcttgccgag
      721 accggtcccg ccgtagatga agagcgggtt gtacgcctgc gccggagcct cggcgaccgc
      781 gagtgccgcc gcatgcgcaa aacggttcga cgagccgatc acgaacaggt cgaacgtgta
      841 cttcgggttc gtgcggggcc cttcgcctgc cgcctccgcc tccacgtgcc cgggccgctc
      901 gaccgagggc gcagacgcgg ccggcaggga ctccgcgaca ctcaggcgaa cccgaacgtc
      961 gcggtcgatc gcgtcacggg ccgcggcctt catcaggccg agaaaatgtc cctcgatcca
     1021 ctcccgggtg aagtcgttcg ggacgacgac ttcgaagacg tcatgcccga gttcgcctgg
     1081 ggcgacggct ccgaaccacg tcgcgtaggt cgtttcgttg agcgtctcgc ggagccgatt
     1141 cgagacttcg tcccacagct gcgcagcgct aggctcgggt gtgttcaccc gtctcgggct
     1201 ccttgtcggg ggggttgcgg gacggcgcga gcatatcagt gtccgggagc gctctcaacg
     1261 cagtccgagc gacctcggcg atggaagaaa aacgcgcgat ttgcaggaag aaagctcctc
     1321 tccacacacg tttccccagg tgtggaaaag catcgaagcg cacgatcgag cggtgcctcc
     1381 tcgcacgtgc tgagcagcgc tttcgatctg cccggatcgg cacaatcgcc tgtggataac
     1441 tgaggcgtgg gcggctcgtc tatactcccc gctcggccgc cgcgagcgcg gcgagcgaac
     1501 ctgcgagcac tcacggatga aacgcacgta ccagccgaac gtccgccggc ggaagcgcaa
     1561 gcacggcttc cgggcgcgca tgtcgtcgcg cgcgggacgg gccgtcatca agcgccgccg
     1621 agcgaggggc cgcaagcggc tctcggcctg atcgcggccc gtgcagcgac ggcagcgcct
     1681 ttctcggtca cgggacttcg acgccgtcta ccggcagggc cggtcggtgt ccacgcggta
     1741 tctgacgctg cactggttcc cgcgtgagga cgacccggac ggcgagccca ggctcgggct
     1801 ggcagtcccg aagaccgtcg gcaaggctgt cacccgcaat cagctgaagc gccgcctccg
     1861 ggcgagctgg acagagctcg gcgagcgggt tccgcgcgga cgcgactacg tgctcgtcgc
     1921 gcggcccggg atcgccgacg ccgtcgaggc tcgcgaccac cagtggttgc tcgagcgcct
     1981 cgacgaagtg cttgggaagt cgtcggcatg aggcacctcg gggtcgcgct cgtctacgcg
     2041 tggcggtatt ccttcggcct gctgacgccg accggaacgt gcaagtacca cccgagctgc
     2101 tcgcagtatg cgatcgacgc gtttcgagcc gagggcttcg tgaagggcgg aatcctcgcg
     2161 acatggcgcc tcttgcggtg caatccgtgg agccacgggg gcgtggacta cgtcgagcag
     2221 caaagactgt tccggcgtcg cgggaaggaa tcgagtgacg ccgcgggcgg tcgtgtctga
     2281 tgctttcgct cctgctcccc tcgctgctgg ccgggatcct cagcccgatc gagaacatcc
     2341 tcacggcgat cctcgagtgg ctgcactcgt cggtcgggct gacctgggcg tggtcgatcg
     2401 tcgccctgac ggttctcgtc cgtatcgcgc tcgtgcctct gacggtgcgc cagatccact
     2461 cgatgcagaa cctgcagatg cacgcgcccg agatgaagga gatccagcag cgctggaagc
     2521 acgacaagca gcggcagaac gaagagctga tgaagttcta ccgcgagaac aagatcaacc
     2581 cggcggcgtc ctgcctgccg atcgtcgcgc agatcccgat cttcatctcg ctgttcttcg
     2641 tcctccggga tttcgagagc gagatcttcc ccaactaccc cgcgtcgagc ctcgagtggc
     2701 tggacctgat cacgatcacg gagcccacgt ctcagggatt gggcccactg ctgctcgtgg
     2761 tctacgtcgc cagccagctg tcgtcctcct acctcatgtc ggggacgatg cagcgcatgc
     2821 agcgcatcct cctgatggtg ctgccgatcg tcttcatccc cttcatcctc cggttcccgt
     2881 ccgggctgat gatctactgg ctgacaacga acctctggac gacgggccag ggcctgatca
     2941 ctcgtcgcct gatcccgaag gcgccgccgc aagaaaagcg cagctcccga acgcccgcca
     3001 aggaagaaga aaaggccccg gccaagtcgc gctcggacga cggcgacgcg cagcgcgcca
     3061 gtggcgacaa ggacaagaag agcaaggcca agcagggtca gaagaaggtc gtcgccggtc
     3121 cgccccgccg cgtcaagcgc aagcgcggcg gccggcgatg agcgacctgc tgaccgcgga
     3181 ggcgaccggc gagacggtcg gcgaggcgaa gtgggcagcc gtgcgcgagt tggagcgtca
     3241 gcttccgggg ctcgaccgaa acgccgttcg gttcgaggtg ctctcggaag gcgagcgcgg
     3301 actgctcggt gtcggctaca cgccggcgcg ggtcatcgcg gcgatcgcga gacccgaggc
     3361 catgccgccg cccgaggacg agtcggccga gtccgctctg gtccgcgagc tgctgcagca
     3421 cgtcgtgtcg ggactcggcg tcgtctgcac gatccacgtc gacgaccacg acgaggagat
     3481 ccaggctgag ctggcgggcc cggatctcgg catcctgatc gggaagcacg gacagacgat
     3541 cgacgcgatc cagtacctcg ccaacgccgc cgcacagcgg cggctcgggc cggagcgccg
     3601 ccgcgtcgtg atcgacgccg gcggctatcg ggagcgacgc acggcgacgc tccagtcgag
     3661 tgccaggcgg agtgccgagc aggcggcggc caccggccgc cccgtctccc tggagccgat
     3721 gacggccgtg gagcggcgga tcgtccacga gtacctgaag gacgatcccg aggtcgagac
     3781 tgcgagcgag gggaccgagc cgaaccggtt catcgtgatc ttcccccgcc gcagcacgta
     3841 aggggagtgc cggccgggtg ctcgaccgct ggctcgccgc ggtcgtgtcg accccaggcc
     3901 tgacgtcgct gcgtgacccc gccgagggac ggcgcgttct gctcgacgac gcgcttcgtg
     3961 cggtggagct ggtgcgcgcg ttgcccggac cgatcgtcga cgtgggctcc ggtgggggta
     4021 cccccgggat cccactcgcg gtgacactgc ccgagcgcag cgtgactcta ctcgagtcgc
     4081 agcggcgcaa ggccgctttc ctggaaggct gggccgagca gctcccgaac gtgcgtgtga
     4141 tccggggacg ggcggaggaa cagcccgtcg acgcctacgg ggtggctctc gcgaaggcgc
     4201 tcgcgccgcc gcccgtggcc gccgagctgt gtctgcctct cgtccgccgc ggcggggcgg
     4261 caattctctg ggttgggcca acggccgatc gcgagcgggt cggccgcgtc gcgacgcagc
     4321 tcgccgccga gttgtccgag ccggcgagcg gtctcctcct cctgcgtaag ctcggcggca
     4381 cgccgccggg gtttccgcga cggcccggaa tggccgccaa gcggcccctc gcatagcgtg
     4441 ccgccggcaa ttcgcgccga ccccatctct acccttcaga cgtgcctcct cgtacgtacg
     4501 ccgtggccaa tcagaagggc ggcgtcggca agacgaccac cgccgtgaac ctggccgcct
     4561 gcctcgccga ggcgggcgag cgctgcctgc tgatcgacct cgatccacag gcgaatgcga
     4621 cgtccgggct cggcgcgaga gcgaacgggt cgtcggccca cgacctgctc gacggtgtcc
     4681 cgttggggga gctgacgaag ccgacgtcgc tggcgaatct cgacctcgtc ccggccaagg
     4741 gcgacctcgc ggcggcggcg gttgagctgt cggtgcgcga gggaggcgag cgctacctgg
     4801 ccgatgcgct ggccggctcg gcgggcgcgt actcgttcgt cttcctcgac tgcccgccgt
     4861 cgttcggccc cctgacggtg aacgcgctcg ccgcggccga tcgggtgatc gtcccggttc
     4921 aggctgagta ctacgcgctg gaggggctct cgcagctgct ggggtcgatc aacctcgtga
     4981 aagcgcgtct gaacccggat ctcgccgtca ccgggatcct gctcacgatg gtcgacgggc
     5041 gaacgcgcct ggcggccgag gtggaagagg agctcaggcg ccacttccgc ggattggtct
     5101 tcaccacgtc cgtgccgcgc tcggtgcgcc ttgccgaggc gccgagccac ggcctccccg
     5161 caatcgccta cgcccggcaa tcggccggtg cgcaggccta ctggaaggtg gcgatggaac
     5221 ttgtcgagcg ctcctgagcg gcccggtgcc gctgcgccgc gccgcggtct cggccgtggc
     5281 ctcgaggtgc tcgtcggcgg aggcggcacg cccgagctgc tgcacctgcc cgtcgagtcc
     5341 atacacccca gcccgcgcca gcctcggcgg cggttcgagc ccgaggcgac cgccgggctc
     5401 gctgcgtcga tccggctcca gggcgtgctg caaccggtag tcgtccgctc ccgccctgaa
     5461 ggcggctacg agctgatcgc cggtgagcgc agatggcgcg ccgctcgaga ggccgacgtg
     5521 ccgacgctcc cggccgtggt ccgcgaggcg gacgaccggg agacgcttct gctcgggctg
     5581 gtcgagaacg tcgcacgtga ggatctgtct cccgtcgaag aggcgcgcgc ctacgcgacg
     5641 ctgatcgacg agttcgaact ctccctcggc gacgtcgccg agcgggtggg ccgctccaag
     5701 ccggccgtct ccaaccgcgt tcgcctgctg gaactcccgg aggaggttct ctggatgctt
     5761 gcccgcggcg agctgaccga gggtcacgcg cgcgcggtgc tggcgttgcc ggaggacgga
     5821 gagcgggttc gtttggcccg gcgtatcgcc cgcgatggaa tgaccgttcg cgccgcggag
     5881 cgggctgcgc gcgagggcgg tgcgcgtaga aagcgtcgcg ccccgtcccc ggtcgatccg
     5941 gcgctcgcag agcgcgcacg ccttgcgctg gaggggctca cgggctgtgc cgttcgtgtc
     6001 cgccgcggag cgctcgaggt cgagttcggc gacgagacgg gccttgccga gctcgtggag
     6061 gcgctggagc ggacggctgc atgacccacc tgggcggaac aggtgggcga tcctggactc
     6121 gaaccaggga cctccgcctt atcagagcgg cgctctaacc agctgagcta ctcgcccggg
     6181 gcggcgaact gtagcgtacg acccggggct aagatggccg cgaggaacgc atgcgagaga
     6241 tgaccgtttt cggagtgagt ttcgacatcg tcggcaagca gccgatcgtg ctcctgaaga
     6301 cgcaggacga gaacaagttc ctcccgatct ggatcggcca cccggaggcg gcggcgatcc
     6361 tgatgaagct gcagggctcc gcgtcgccgc ggccgatgac ccatgatctc ctgacgagca
     6421 tcctcgatca gctcgaggcg gagatcgtgc gaatcaccgt caccgagctg cgcgagaaca
     6481 ccttctacgc ccagatcaca attctccaga acggacacga gatcgaaatc gactcccggc
     6541 cctcggatgc gatagcgctg gccgttcggg ccgacgcgca gatctttgcg agcgaagagg
     6601 tgatcgagga gtcgggcatc gagttcgagg gtggcgagga ggaggctcag agtcagatcg
     6661 cgcgcgtctc ggagctggcc gatctcgacc cggaggagtt ccggcgcttt ctggacgagg
     6721 tcacgcccga gcagttcgcg gaggggttgc ccgaaggaga ggagcccgaa ggagaggagc
     6781 ccgaagaaga gactgggccc gaagagcgtg agggcgagga ggacgcctaa gcgagctcgc
     6841 gcttcgtcag ggccagcagc gccgacacga gctcgtccag gccctcttcc gagaggggac
     6901 cggggttttc cgcccgcagt gcgtcccgca gcgctcgctc acgctcgggg tcgacgcgat
     6961 cgagcccctg ttcgagcttg agctgccaga gctcgcgaac gagagtaagg cgctcgttga
     7021 ccgcggcgac gatctcccga tcgttctggg cgatcagaaa tcgcagctcc tcgaagcgtt
     7081 cgctcacagc tccgtcccga gggtcacggc cgccagcagt gcggctccgt tgaagaacac
     7141 gtgcatcagc gtagacgggt agaggctgtc cgtatcgcgg cgcagccagg ccgcggcgag
     7201 accgaacacg gtgagcgcgt ggaggccggc gacgagaccg tgcgagagcc cgaacatgac
     7261 cgaggtgacg agaacggcgg cgaggagccc gtacacggcc cggacggccc agtagccgaa
     7321 gccacggtag gtcagctcct cgacgaccgg cccgaagaac gcgatcacga tgaagttggc
     7381 gatgaaggcg ccggctcgat ccggctccca agcctcggga acgatgccct gttcgtcgcc
     7441 ggcgcgaagg tcgagcacga acgtgaagac gacgttcgcg gcccacaccg cgattagtgc
     7501 gaccaccatc ttgacgagcg ccgagctcca ggagtcgggg cgccggagcg cgaagacctc
     7561 gcgggccggt agccccctgg ccagaaagac gacgatcgtc agcaagatca ggtagaagac
     7621 ggcaccgctg atcgcggtcg ggtactcgta gagcacgttt cggcggtcag tgtcgtcttc
     7681 ggtggcgaag cgggtcgcgt agttgaggag ggcgaccacc ccgaccaggg caacccagcc
     7741 ccagaggcgc ccgcgcggta ggagctcggt tgccgagccg acgctcatcg atgcatttga
     7801 ccatgccgct gcgggatcgc gccgcgactg gtgccgccta agggtttctc gagcggagcg
     7861 gaacccgcct gccgacgccg aaacaggccg agtgggcagc gtgaccctgg ctaccatcgg
     7921 cgcatgacac cgcccactcg aggcacgccg aggtcgggcg agctctttcg cgtgtcccgc
     7981 agacgccggc gccgcagggg caaccggcgc cgtgggcggc gcggcctcgt cgtcacgctg
     8041 gcggccttcg cggtggtgct gatcgcggtc ggggtcggca ccggcgccgc ggttctggca
     8101 ttcggggaat cgtgcgacct gtcctcgttg aaacccgtgt cgatcggcca gaactcgttc
     8161 gtctatgcgg ccgacggatc gttgctcggc gccatcccgg ccgagcgaaa ccggcagccc
     8221 gtggtgctgg cgaaggtcag cccgtggatg cgcaaggcaa cgatcgcgat cgaggatcga
     8281 cgcttctacg accacggggg actcgacctc gagggcatcg cacgggcggc gtgggagaac
     8341 gtgaaggccg gcgagttggt gcagggcggc tccacgatca cgcagcaact cgtccgcaac
     8401 ctctacatct cgaacgagcg caccgtcgag cgaaaggtga aggaagcctg cctggcgctg
     8461 aagctgaacg actcgtggtc gaaacgacgg atcctggcga cgtatctcaa ccaggtgtac
     8521 tacggcaacc ttgcctacgg cgtcgaagcg gccgcccaga cctacttctc gaagtcggca
     8581 gccgacctcg atctccgtga ggccgccctg ctggccgggc tgacgcaggc gccgacgtcg
     8641 tacgatccgt tgacgcaaga gcagcgcgcc cgcgtgcggc ggacacaggt gctgcgtgcc
     8701 atgcgcgaga ccggggcaat cacgacgcct cagttccggg ccaccgtcaa ccggccgttg
     8761 gggctcaagc cgggccgcct ctaccaggag atccgcgagc cgtacttctt cggatacgtg
     8821 cgcgacgagt tgatcgcgga gtacggtgcc gagaccgtcc gttcgggcgg tcttcaggtg
     8881 ttcacgacgc tcgtgccgcg cttccagcgc ctcgctcgtg cggcgatcag cgagacgctg
     8941 accgagccca cggacccggc ggcggcgatc atctcgatca atccgcgtac gggtgcgatc
     9001 agggcgatga cggcggtgac gccgggccgg gcgcagaacg agttcaacct cctctcgcag
     9061 gcgcgccgtc agcccggctc gaccttcaag acgttcgtgc tggcggcggc ggtcgagcag
     9121 gggatcaacc cggcgacctc ctactacgtc tcggcaccct tcacctaccg tcccgaggcc
     9181 acgggcaact gtgacgacgg gtcgtggtgg tgcgtcaaga cctatgactc cagctacggc
     9241 ggctggacgt cggtcgagcg cgcgacgttg cgctccgaca acgccgtgta tgcgcagctc
     9301 acgctcgacg tgacccctca gcgggtcgcc gagacggcgc gaaagatggg cgtccggacc
     9361 ccgctggacg tgaacggcca gtacgtcccg gcgatgggac tcgggtcgat cgcggtgtcg
     9421 ccgctggaca tcgcctccgg ctacgcgaca ctggccgcgg gcggcgtcca tgcggagccg
     9481 agagcgatcc ggcgggttgt cctgccgaac ggcaaggaag acaccgatgc cggatggggg
     9541 aagccgcgcc gcacgcgcgt gctctccgac ggcgtcgcag cggccgtgac ggagatcctg
     9601 gccgacaacg tgctgtacgg gacgggaacg cgcgcctact tcggccgtcc cgccgccgga
     9661 aagaccggca ccaccgacag gcatgcggac gcctggtttg ccggatacac gccgcagctg
     9721 cagacgaccg tctgggtcgg gtatccgcgc ggcgagatcc cgatggaaaa cgtccacggt
     9781 atcgccgttt ccggtgggag ctttccggct gcgatctggc gtctcttcat ggagcgggcg
     9841 ctggcgccgg tggcgccggc agacttcccg gagccgctcg atcagcccga gtggacgacc
     9901 ttcgtccgcg gcccacatgc gctcacctac gaccccttct acaagccgcc cgaggaagac
     9961 gaggagaagc aggacgaggg cgggaaggag caagacacgc cgggcggcga aggggctgac
    10021 gcgggtgacg acgacgacgc cggcggctcc accggtggtg gagccacgag cgactctccc
    10081 gggccgggcg gggcgacggg cgccggcgtg agcgagacgc cctgacgctg gggcacccca
    10141 acccaccacc cgctgtcatc ctatcgtcag aactgacaac ggtggtgggc gaattggtgt
    10201 gggaagtgtg acgttttgcc ggggggcggg tagggtcgcc gcgtggccga tctgctgacg
    10261 cgggaggagg cgctcgctcg gattctcgaa cgcgcccggc ccttggcggt ggaggagacc
    10321 ccagcagcga acgcggccgg ccgcgtcctc gccgccgctg cggtggccgt gaccgatctc
    10381 cccccattcc ccagctcggc gatggacggg tttgcgcttc gcgcggccga cgcgcccgcc
    10441 acgctttcgg tcgtcggccg ggttgctgcc gggcgcccgg cgcgccggag cctcgccgcc
    10501 ggagaggcga tgggcatcgc aacgggtggc actgtgccgg aaggcgccga cgccgtcgtg
    10561 ccgatcgagc tggtggtcga tcatggcgcg acggtcgacg tgccggagcc cgtcgcgccc
    10621 ggacagtacg tgcgcccgcg cggcgaagac gtgagggcgg gtgggcaagc gctgcccgcc
    10681 gggacgcggc tcggcgccgc ccaactggga gcgcttgccg cagccggcat cagccacgtg
    10741 gcctgctctc gacggcctcg cgtggcgatc ctggcaaccg gcacggagct acgcccgccg
    10801 ggcgagcctc ttgggcccgg cgagatctac gaatcgaacg gggtgatgct ggaggtcctc
    10861 ttcgcgcaag ccggggcaat ggtcgagcgc ctacagtcgg tccgcgacga cgagcggtct
    10921 catcgagcgg cgatcgagcg gggcctggct gcggacgtac tggtcacctc gggcggagtc
    10981 tccgtcggcc cgcacgatct cgtgcgggcg actgagcggg cgcttggggt cgcggagatc
    11041 ttctggggcg tcgcggtccg gccgggaaag cctgtctcgt tcggcgttcg aggagacacc
    11101 ctggtcttcg gcctgccagg caacccggtc tcgtccctcg tgggcgcgga gctcttcgtc
    11161 cgccctgccc tgcgggcgct cgaaggcgac tcgcagcccg gcccccactt cgagctcggc
    11221 acgctagcgg agtcggtgca gccgcacccg cgccgcgacg atctcctccg cgtgcgaagg
    11281 ctcttcgaga acgatggggt tcggctcgct ccgatcgagg ggcaggggtc gcacatgatc
    11341 gtccgcggcg ccgctgcaga cgcgctggct ctgatacccg cgggagaatc cacgatgccg
    11401 cccggctcgc cggtgcgtta tctccgcctc gggtagaacg accttccggc ggcccgaaca
    11461 cgtgcgagta gcggcggcgc atcggcacgc ggcagtgaat agcgccccga ggccactccg
    11521 ccgacgtgca ttgcgaccgc cccccacgcg agcacgatga cgacggtcgc ccaggagagg
    11581 tagccgacgg tcaccaaccc cgacacgtac aggagcacct cgagcccgag gccgaagcgg
    11641 tctgcgaggg agacgagcgc gagcagcgga cccgcgaatc cgagaccgta gaggacgtcg
    11701 cgcgcccacg aacgtccggt ctgcggaagc caaagccatg catacagcga cggcacgacg
    11761 aagatgaggg agtaggtgtt cactacggct gcggcgagag cgacgattcc gagcgccgcg
    11821 agaccggcgc cgtagcccgc cagctcctcc tccggtgagg gaggtcgcca cgcgccccgc
    11881 tgcctgcggg tcaacagcca cggcacgacg atcagcagcg acagggcggc gagtgagcca
    11941 aaccgccagt cggtggccga cgggcccgac ggcggcaagg gccgcgcgga tccggtcggt
    12001 agggcgccga gcagcgcggc cagccacagg acgacggcag cccacagcca aagagcgagc
    12061 ctgccgagca gccccgcgaa cgccggctgc agcggaatag ccatgcggcg gcaccgcacg
    12121 agaaggtcga cgacgccgac gccgaagggg acgaccgccg ccaggagcac gagcgcgatc
    12181 gtccagccgc gcacgaccct cccgccggcg tacacgtatg cggacgtgcc gcgcgccagc
    12241 tcggcgccgc tgtcgaggga gcccaagaga gactgggcgc cccggcccag cctttccatc
    12301 cgggcagcgt tcagctcggc ggtcgtgtcc gttcccgccg ccgcgccgct gtcgtccgca
    12361 gccgtcacgc gcagcgcgga gatccgctcg cccaagaacg gagcctgatc gccgtacgcg
    12421 aacggaatcc cgaggtcgac cagctggcgg acgatccctg gccgcgccgg ctcccggccc
    12481 agctcctcgc cgaggcgcgc gcccgcggtt cgaacgaggg tgggtgccgg cgagcgcggt
    12541 ccgtcgcccg cgagctcgag acgcggctcg cccgggcccg acagagcttc gagcacgacg
    12601 accgcactca cgttctcacg aaagcgagac gtcgacgcga accgcgctgc tcccaggccc
    12661 ccgaactcac cagcgtccgt cgacaggaac accaacgtct g
//
LOCUS       DBNGNK010000102         7262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1809188,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000102 DBNGNK010000000
VERSION     DBNGNK010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1809188"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="AC150V_02885"
     CDS             <1..558
                     /locus_tag="AC150V_02885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3761897.1"
                     /translation="WLLVAIFALMASAYYGINSWIPDSLVERDWDKENAGAVLAVLNT
                     VAIPAAFIVPWLSEKRGGRRPWLIVMGGFFLAGVVGFAVAPDASYLWACSVGVASGSM
                     FSLVMTLPLDLADRPALVGALAAMMLGVGYTVGAISPLVLGAVRDATGTFTGSLWLLV
                     AFSAGLIALVAMLPRPRAGPAEVAA"
     gene            complement(563..2026)
                     /locus_tag="AC150V_02890"
     CDS             complement(563..2026)
                     /locus_tag="AC150V_02890"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3761898.1"
                     /translation="MSETLTGTPAKNYVGGEWRDSVSGETYEKRNPWRPSEVTGVYPA
                     SDAEDARAAVAAAQGAFPTWAELTSAQRAAVFHKTADAIEARAEQVAQDMTAEMGKPL
                     REARMETARAAAILRFSAGEAWRPIGDVYEPSFANQNLYTKRRPLGVVGLITPWNFPI
                     AIPVWKLAPALIYGNTVVLKLGYEAPRTGLHVAECFAEAGLPAGVLNVVTGAGSKVGA
                     ELVSNPGVRAISFTGSVPVGKSVRNEATARDCRVQLELGGHNPLIVMADAELERAAEA
                     AYAGAFWSAGQKCTATRRIYVQDDVYDRFRELFLRRIDKGVVGDPSDPETEVGPVVNE
                     GPMEEILDAIERSRGDGGTVIAGGERASEDGFLIEPTVFEGLADDAYLSCEEVFGPVT
                     SLYRFSTLDEGLERANNVPFGLSASIFTRDLHAVQRFSDELQAGILHVNSQTAGADVH
                     VPFGGIKSSGWGPHEQGRAAIEFYTEFVTVYQDAPLD"
     gene            complement(2029..2856)
                     /locus_tag="AC150V_02895"
     CDS             complement(2029..2856)
                     /locus_tag="AC150V_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3761899.1"
                     /translation="MLSPGRHVRFDGPGGVEHGIVDDGVVLTASGERIELLDPATVSL
                     PEPYELVLPIEAPEVWCAGVTYERSRDARTEESGSDVYALVYDSDRPELFLKDAACRR
                     TVGPGGTIGVRADSTWNVPEPEIGLVLGENGAIAGYTIGNDVSSRDIEGTNPLYLPQA
                     KVYAGACAIGPTIFVPDGPAPTFAIELRIFDAEGAEVFAGETSSGRMTRSFADLVDWM
                     LRDNPVPPGSVLLTGTGLVPPDEFTLAPGHRVEITVPEIGTLANPVSSATKLLERSS"
     gene            complement(2856..3581)
                     /locus_tag="AC150V_02900"
     CDS             complement(2856..3581)
                     /locus_tag="AC150V_02900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761900.1"
                     /translation="MSNDNRFSGRVGLVTGASSGIGAAVAERLVREGAKVASLDLSGS
                     APAGVLPLTGDVSRSEDVGAAVAETESELGPIDVLVCAAGIPGASLRTVDVSDEEWRR
                     VLAINTDGVFFCNRAVIEGMVDRGYGRIVNLASIAGKEGNPMAAAYSASKAAVIALTK
                     AIGKDLARTGVAVNCIAPAVIETPILAGISQEHVDYMVERIPMGRVGRADEVAALVCW
                     LASEECSFSTGATYDISGGRAVF"
     gene            3612..4019
                     /locus_tag="AC150V_02905"
     CDS             3612..4019
                     /locus_tag="AC150V_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase small subunit"
                     /protein_id="HIR3761901.1"
                     /translation="MDFDPKRDYPLGLRRSDLLTTPGGTGFDDVTLGAARAGEIDSAD
                     MRSTAATLRRQAEVARAAGRAQLADNLERAAELAAVPDGELLEIYTALRPGRSSAAEL
                     ETRARALEDYGAPKTAAFVREAAAAYTERDLLA"
     gene            4012..6285
                     /locus_tag="AC150V_02910"
     CDS             4012..6285
                     /locus_tag="AC150V_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HIR3761902.1"
                     /translation="MPESGRLASRAEKELRRELLVSPLPELGLVAANGPDDPEPELVV
                     EHGRVVRMDGRAEADFDVIDRFLAAHGFDLAMAAEAMAQTDEQIARALVDVDVPRDEL
                     VRLARGLTPAKLARVLGSLDPVELMFALKKLRARRDPGNQAHVTNLKENPALLAADAA
                     EAARRGFDEMETTVGVARYGPLNALALLVGSQTGRPGAMTQCAVEERRNLELAIRGLV
                     TYAETLSVYGTEPVFVDGDDTPWSKSFLAAAYASRGVKVRFTSGTGSEALMGHAEGKS
                     MLYLEARCVSVVRAAGSQGIQNGSISCVALALSVPGGTRAILAENVLAAWLDLEVASG
                     NDAIASHSAIRKTAKLMGQFLPGTDFVTSGYSVMPRRDNTFGGGNYDADDIDEWLTIQ
                     RDWQIDAGIEPVPDDEVHAVRERGARAVQAVFEELGLPPVTDAEVDAATKAYDSRDLP
                     DRDRAADVEAADAMLERGVSGLDVALALDRRGFGDVAEAVFGMQRQRIAADYLQTSAV
                     IEPDGLVRSAVNEPNVYLGPGTGYRLEGERWQLLQALPHVSDPARLGRGEVGGAPLFE
                     ELGIAEPGAEPAEVVVAVGPAYGAEIVETITGLDHEAVLAAIDEGIREAGATPRFVRV
                     RRVADVGFIAHDAARLAGSGIGFGLQSKGTAVIHRADLEPLDNLELFGMSPLYSLESY
                     RAMGRNAAGYALGQRVGPVPTELDNFARAKLIVRTTLLHARETRAVVPGAAPVELVPA
                     HLAGDGSRPAKPGSAVA"
     gene            complement(6328..6510)
                     /locus_tag="AC150V_02915"
     CDS             complement(6328..6510)
                     /locus_tag="AC150V_02915"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HIR3761903.1"
                     /translation="MPIIDVKLFDKRVTEESVPKIIEALTNALAESSGAATEHIQVIV
                     QGVSPQHWGQAGKPMA"
     gene            6577..>7262
                     /locus_tag="AC150V_02920"
     CDS             6577..>7262
                     /locus_tag="AC150V_02920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3761904.1"
                     /translation="MRYRRLGRTDLAVSEVGFGAWGIGRFMWIGADDDESLRALHRSF
                     ELGVNFVDTALGYGDGHSERLVGAAVRETDETVYVATKIPPKNFQWPAANGVDPRQTF
                     PADWVVAKTEESLGNLGTEAIDVQQFHVWSDEWVGRGDWLDAVDRLKRDGKIRFFGIS
                     VNDSQPANALRLLETGVVDTVQVIYNVFEQAPEDELFGAVDVADVGVIARVPFDEGSL
                     AGQVRPDTEFP"
BASE COUNT         1063 a         2496 c         2546 g         1157 t
ORIGIN      
        1 tggctcctcg tcgccatctt cgccctcatg gcctccgcct actacgggat caacagctgg
       61 atccccgact cgctcgtgga gcgcgactgg gacaaggaga acgcaggcgc ggtgctcgcc
      121 gtcctcaaca cggtcgcgat cccggcggcc ttcatcgtgc cctggctctc cgagaaacgg
      181 ggcgggcgac gaccgtggct gatcgttatg ggggggttct tcttggcggg agtcgtcggc
      241 ttcgcggtcg cgccagacgc ctcctacctg tgggcgtgct cggtcggggt cgccagcgga
      301 tcgatgttct cgctcgtgat gacgcttccg ctcgacctcg ccgaccggcc ggcgctggtc
      361 ggcgcgctcg ccgcgatgat gctcggtgtc ggctacacgg tcggcgcgat cagtccgctc
      421 gtgctcggcg cggtccgtga cgcgaccggc acgttcacgg ggtcgctctg gcttctcgtc
      481 gctttcagcg cagggctgat cgcgctggtc gcaatgctgc cgcggccgag agcggggccg
      541 gccgaggtcg ctgcctagac ggctagtcga gcggcgcgtc ctggtagacg gtcacgaact
      601 ccgtgtagaa ctcgatcgcc gctcggccct gctcgtgggg gccccagccc gagctcttga
      661 tgccgccgaa cgggacgtgt acgtcggcgc cggccgtctg cgagttcacg tgcaggattc
      721 cggcctggag ctcgtccgag aagcgctgca cggcatggag atcgcgcgtg aagatcgacg
      781 cggacaggcc gaagggcacg ttgttggcgc gctccagccc ctcgtcgaga gtggagaagc
      841 gatacagcga cgtgaccggg ccgaacacct cctcgcagga cagataggcg tcgtccgcga
      901 ggccctcgaa gaccgtcggt tcgatcagga atccgtcctc gctcgctcgc tccccgccgg
      961 cgatcacggt cccgccgtcg ccgcggctgc gctcgatcgc gtcgaggatc tcctccatcg
     1021 ggccctcgtt cacgaccggg ccgacctccg tctccggatc cgaggggtca ccgacgaccc
     1081 ctttgtcgat ccggcggagg aagagctcac ggaaccggtc gtagacgtcg tcctggacgt
     1141 agatgcggcg ggtggcggtg cacttctgcc ctgcagacca gaacgcgccg gcgtatgcgg
     1201 cctccgctgc acgctcgagc tccgcgtccg ccatcacgat cagcgggttg tggccgccca
     1261 actccagttg cacgcgacag tcgcgggcgg tcgcctcgtt ccgcaccgac ttgccgacgg
     1321 gcacggagcc ggtaaaggag atcgcccgga cgcccgggtt ggagacgagc tccgcaccga
     1381 ccttcgaccc cgcgccggtc acgacgttca gcactccggc gggcaacccg gcttccgcaa
     1441 agcactccgc cacgtggagg ccggtgcggg gcgcctcgta gccgagcttc aggacgacgg
     1501 tattgccgta gatcagcgcg ggcgccagct tccagaccgg gatcgcgatc gggaagttcc
     1561 acggagtgat caggcccacc acgccgagcg gccgccgttt cgtgtagagg ttctggttgg
     1621 cgaaggaggg ctcgtagacg tcgccgatcg ggcgccacgc ctcgcccgcg gagaaccgca
     1681 ggattgcggc tgcgcgcgcg gtctccatgc gtgcctcgcg gagcggcttc cccatctcgg
     1741 ccgtcatgtc ctgcgcgacc tgctccgcgc gagcctcgat cgcgtccgcg gtcttgtgga
     1801 acacggccgc ccgctgcgcc gaggtgagct ccgcccatgt cgggaaggct ccctgcgcgg
     1861 cggcgacggc ggcacgagcg tcttcggcgt cggaggccgg atagacaccg gtgacctcgg
     1921 acgggcgcca cggattgcgc ttctcgtagg tctcgccgga gacgctgtcg cgccattcgc
     1981 cgccgacgta gttcttggca ggcgtcccgg tcagtgtctc ggacatcgtc aagagctcct
     2041 ttcgagcagt tttgtcgctg aggaaacggg gttggccaat gttccgatct cgggcacggt
     2101 gatctccact cggtgccccg gcgcgagcgt gaactcgtcg ggcgggacga gcccggtccc
     2161 ggtgagcagc acgctacccg gtggcacggg gttgtcacgc agcatccagt cgacgagatc
     2221 ggcaaacgac cgcgtcatcc tgccgctcga cgtctcgccg gcgaacactt cggctccttc
     2281 ggcgtcgaag atgcggagct cgatcgcgaa ggtcggtgca ggcccgtcgg gcacgaagat
     2341 cgtcggcccg atcgcgcagg cgcctgcgta aaccttcgcc tgaggcaggt agagggggtt
     2401 tgtgccctcg atgtcgcgcg aggagacgtc gttgccgatc gtgtagcccg cgatagcgcc
     2461 gttctcgccg agcacgagac cgatctccgg ctccggcacg ttccaggtgg agtcggcacg
     2521 cacaccgatc gtcccacctg gcccgaccgt gcgcctgcac gccgcgtcct tgaggaagag
     2581 ctccggccgg tcggagtcgt agacgagtgc gtagacgtca ctgcctgact cctcggtccg
     2641 cgcgtcacgg ctgcgctcgt acgtgacgcc ggcgcaccag acctcgggcg cctcgatcgg
     2701 gagcaccagc tcgtacggct ctggaagcga gacggttgcg gggtcgagca gctcgatccg
     2761 ctctcccgag gcggtaagaa cgacgccgtc gtcgacgatt ccgtgctcga cgccgccggg
     2821 accgtcaaac ctgacgtgcc ggcccggaga cagcactaga acacggccct gccgccgctg
     2881 atgtcgtagg tcgcgccggt cgagaaggag cactcctcgc tcgcgagcca gcagaccagg
     2941 gcggcgacct cgtcggctcg gcccactcgg cccatcggaa tccgctccac catgtagtcg
     3001 acgtgctcct gtgagatgcc cgcgagaatg ggagtctcga tcacggcggg ggcgatgcag
     3061 ttcaccgcga caccggtgcg ggccagatcc tttccgatgg ccttcgtcag ggcgatcacc
     3121 gccgccttgg aggccgagta ggcggccgcc atcggattgc cctccttgcc tgcgatcgag
     3181 gcgaggttga cgatgcggcc gtacccgcgg tcgaccatcc cttcgatcac cgcgcggttg
     3241 cagaagaaga cgccgtccgt gttgatcgcc aggacgcgtc gccactcctc gtcgctcacg
     3301 tcgacggtgc ggagggacgc gccggggatg ccggccgcgc acacgagcac gtcgatcggg
     3361 cccagctccg actcggtctc cgcaaccgcg gccccgacgt cttcagagcg gctgacgtcg
     3421 ccggtcaggg ggagcacgcc cgcgggagcg ctccccgaca ggtcgagcga tgccaccttg
     3481 gcgccctcgc gaacgaggcg ctccgctacc gcggcgccga tcccgctcga cgccccagtg
     3541 acgaggccga cgcgcccgga aaatcgatta tcgttgctca tgtcgtcagt cgaaccctaa
     3601 agtctgtggc gatggacttc gatccgaagc gagactaccc gctcggccta cgccgttcgg
     3661 acctactgac gacccccggc ggcacgggct tcgacgacgt gacgctcgga gctgctcggg
     3721 ccggggagat cgactccgct gacatgcgct cgacggcggc gacactccgc cgtcaggccg
     3781 aggtcgctcg tgcggcgggc cgcgcccagc tcgccgacaa cctcgagcgg gcggccgagc
     3841 tcgctgcggt gccggacggc gaattgctcg agatctacac ggccctacgt ccgggccggt
     3901 cgagcgccgc cgagctcgag acgcgtgcgc gtgcgctcga ggactacgga gcgcccaaga
     3961 cagccgcctt cgtgcgggag gccgcggcgg cctacacgga gcgtgatctc cttgcctgag
     4021 tcgggcaggc tcgcgtcgcg ggccgagaag gagctccgcc gcgagctgct ggtctcgccg
     4081 ctgcccgagc tgggtctggt ggcggcgaac ggcccggacg atccggagcc cgagctggtc
     4141 gtcgagcacg ggcgagtcgt caggatggac ggccgcgccg aagccgactt cgacgtgatc
     4201 gaccgcttcc tcgccgcgca tgggttcgat ctggcaatgg ccgccgaggc gatggcgcag
     4261 accgacgagc agatcgcgcg ggcgcttgtc gacgtcgacg tccctcgcga cgagctcgtt
     4321 cgtctcgcca gaggcctcac gcccgccaag ctcgcacggg tgctcggttc cctggatccc
     4381 gttgagctga tgttcgcgct caagaagctc cgcgcacggc gcgaccccgg caaccaggcg
     4441 cacgtcacca acctcaagga gaatcccgcg ctgctcgcgg cggacgccgc agaggcggcc
     4501 cggcgcggct tcgatgagat ggagacgaca gtcggcgtcg cccggtacgg gccgctcaac
     4561 gcgctcgcgc tgctggtggg ctcgcagacg gggcgtccgg gcgcgatgac ccaatgtgcg
     4621 gtcgaggagc ggcgcaacct cgagctcgcg attcgaggtc tcgtcacgta cgcggagacg
     4681 ctgtccgtct acggaacaga gccggtgttc gtcgacggtg acgacacgcc gtggtcgaag
     4741 tccttcctcg cggccgcata cgcatcgcgg ggcgtgaagg tgcggttcac ctcgggaacc
     4801 ggctccgagg ccctgatggg ccacgcggag ggcaagtcca tgctctatct cgaggcgcgg
     4861 tgcgtctcgg tcgtgcgggc ggcggggtcg cagggaatcc agaacggatc gatctcctgc
     4921 gttgcgctcg ccctctccgt gcccggcggc acgcgcgcga ttctggccga gaacgtgctc
     4981 gccgcctggc tcgacctcga ggtcgcctcc ggcaacgacg cgatcgcctc ccattccgcg
     5041 atccggaaga ccgcgaagct gatggggcag tttctgccgg gaacggactt cgtcacgtcg
     5101 ggctactccg tcatgccgcg gcgcgacaac acgttcggcg gcggcaacta cgacgccgac
     5161 gacatcgacg agtggctgac gatccagcgt gactggcaga tcgacgccgg catcgagccc
     5221 gtacccgacg acgaagttca cgccgtgcgt gagcgcggcg cccgggccgt gcaggccgtg
     5281 ttcgaggagc tggggctacc accggtcacg gacgccgagg tcgacgctgc gaccaaggcc
     5341 tatgactcgc gcgatctgcc cgaccgcgat cgcgccgccg acgtcgaggc ggccgacgca
     5401 atgctggagc gcggtgtgtc cggcctcgac gtggcgctgg cgctagaccg gcgcggcttc
     5461 ggcgacgtcg ccgaagctgt cttcgggatg cagcggcagc ggatcgcagc cgactacctg
     5521 cagacgtcgg cggtgatcga gcccgacggc ctcgtccgct cggcggtcaa cgagccgaac
     5581 gtctacctcg ggccgggaac cggctatcgg ctcgagggcg agcgctggca gctcttgcag
     5641 gcgctccctc acgtctccga ccctgcacgt ctgggccgcg gcgaggtcgg cggagcaccg
     5701 ctcttcgagg agctgggcat cgcggaaccg ggagcggaac cggccgaggt cgtcgtcgct
     5761 gtggggcccg cctacggcgc agagatcgtc gagaccatca ccgggctcga ccacgaggcc
     5821 gtgctggcgg cgatcgacga ggggatccgc gaggccggcg cgacgccgcg cttcgtgcgc
     5881 gtgcgcagag tcgccgatgt cggcttcatc gcccacgacg ccgcccggct cgcgggctcc
     5941 gggatcggct tcggtctgca gtcgaagggc acggcggtca tccatcgggc ggatctcgag
     6001 ccgctcgaca acctcgagct gttcggcatg agtccgctct actcgctcga gtcctaccgc
     6061 gcgatggggc gcaacgctgc gggctatgcg ctcgggcaga gggtgggccc ggttccgacc
     6121 gagctcgaca acttcgcccg cgccaagctc atcgtccgga cgacgctcct gcatgcccgc
     6181 gagacgcggg ccgtggtccc cggcgctgcg ccggtcgagc tcgtcccggc gcatctcgcg
     6241 ggcgacgggt cacgcccggc gaagccgggc tccgccgtcg cctgacatcg cgtcgcgcag
     6301 cttgccgagc cggaggtccg gcccggccta ggccataggc tttccagcct ggccccagtg
     6361 ctgcggcgac acgccctgga cgatcacctg gatgtgctcg gtagccgcgc cgctcgattc
     6421 cgcgagcgcg ttcgtcagtg cctcgatgat cttcgggacg gactcttcgg tgacgcgctt
     6481 gtcgaacagc ttgacgtcga tgatcggcat cgctcctcct tcgtgggtaa gcgccatcgt
     6541 acgcctacgg cggctccgcc tgtatagggt cggcggatgc gctaccggag actcgggcgg
     6601 acggatcttg ccgtgtcgga ggtcggcttc ggcgcctggg gcatcggccg attcatgtgg
     6661 atcggggccg atgacgacga gtctctgcgg gcgctgcatc gatcgttcga gctcggagtc
     6721 aatttcgtcg acacggccct cgggtacggg gacggccaca gcgagcgact ggtcggtgct
     6781 gcggtgcggg agacggatga gacggtctac gtcgcgacga agatcccgcc caagaacttt
     6841 cagtggccgg cagcgaacgg tgtcgatccc cgccagacct tcccggccga ctgggttgtc
     6901 gcgaagacgg aggagagcct cggcaatctc gggacggagg cgatcgacgt ccagcagttc
     6961 catgtctggt cggacgagtg ggtgggccgc ggcgactggc tcgacgcggt cgatcgcctc
     7021 aagcgcgacg ggaagatccg cttcttcggg atctcggtca acgactccca gcctgcgaat
     7081 gcgctgaggc ttctcgagac gggggtcgtc gacaccgtcc aggtgatcta caacgtcttc
     7141 gagcaagcgc cggaggacga gttgttcggg gcggtcgacg ttgccgacgt cggcgtgatc
     7201 gcccgggtgc cgttcgacga gggctcactt gccggtcagg tgcgccccga cacggagttc
     7261 cc
//
LOCUS       DBNGNK010000103         5522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_376103,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000103 DBNGNK010000000
VERSION     DBNGNK010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_376103"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="AC150V_02925"
     CDS             complement(<1..789)
                     /locus_tag="AC150V_02925"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761905.1"
                     /translation="MSEPQRVAVVTGASRGIGYGVAEALGAEGAHVVLVAQDEARLSD
                     AARRLGERGIETEARACDLGDRAATNALADSLASEYPSIQALVNNAGVAELVPIAETS
                     DATWDRILEINLSAAFVLSRGLIRSLERSRAGAVVNVSSVMGLRTIAGLAPYAASKAG
                     LQHLTESLAVELGPRGIRVNAVAPGFIRTDMFEEAHPPERQKVLGHAHPLGRIGSIEE
                     VADVIAYLCGSRSSFVNGAVVPVDGGLVAALAIPRLLEETDERDS"
     gene            complement(852..1376)
                     /locus_tag="AC150V_02930"
     CDS             complement(852..1376)
                     /locus_tag="AC150V_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761906.1"
                     /translation="MAIGPVQLLVFGFEKPEFTGEIAAELVRLRDNGIIRLIDGVVVN
                     KDAEGDVEILQASDLSLEEAEEFGATVGALIGLGMDGEEGFVEGAEAGAEAMAERGGH
                     ALGDPDEWYVVDEIPPETAAVVLLIEHVWAIPLRDKIMDKGGIPLGDAWIRPLDLVAL
                     GVVAEAEAHAQMTA"
     gene            complement(1397..1675)
                     /locus_tag="AC150V_02935"
     CDS             complement(1397..1675)
                     /locus_tag="AC150V_02935"
                     /inference="COORDINATES: protein motif:HMM:NF021378.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR3761907.1"
                     /translation="MARAAMVGGAAYYGGKKSAQGQQREAEQEARIAELEAQQAGPAQ
                     PAAPQAAASAATSMDEKVEELTKLKGLLDAGVLTQDEFDAQKQKILNS"
     gene            complement(1784..3091)
                     /locus_tag="AC150V_02940"
     CDS             complement(1784..3091)
                     /locus_tag="AC150V_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3761908.1"
                     /translation="MIEIGVFALLIIAYGLVSGRLERTPITAPMVFVAVGLVVAAAGG
                     VDFGAVVEEAAGGGADRVTIGELLLTTAEITLALVLFTDAARIDLRKVSRNAAIPGRL
                     LAVGLPLTIALGGVAALLLFDDELIFWEAFVLAAVLAPTDAALGESIVSNPRLPVRIR
                     QSLNVESGLNDGLDVPLFTIFVVLAVAEEEVTAASAGQLILEKLGLGLAVGVAIGAVG
                     ALLLVRSVRRGWVTGVFRQLVVAAMAVSAWWVAEEIGGSGFIAAFVGGVAAGAIVRET
                     AVDFTEDIGQLLNLFVFYAFGAVAYQLLELTTWKTVLYAVLALTVLRMLPVAISLVGT
                     GLQARTVLFLGWFGPRGLASIILAVIVVADESDLAGLETIVVTMTVTVLLSVFAHGIS
                     AAPLARRYAGWSEGMHEEAPEMAGVPELPTRLGMVGDEPSRPG"
     gene            3281..3670
                     /locus_tag="AC150V_02945"
     CDS             3281..3670
                     /locus_tag="AC150V_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006866689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR3761909.1"
                     /translation="MVATSFLDVLWTMLLVFAWIAFFMLLFHVLIDLFRRHDASGWKK
                     TAWVVFLIFVPFIGIFAYLIVNGQGMAERSARQIQAQQEAADEYIRSVAGSSATEQIA
                     RAKELLDQGAISQEEFDHIKTKALASA"
     gene            complement(3681..4703)
                     /gene="hypE"
                     /locus_tag="AC150V_02950"
     CDS             complement(3681..4703)
                     /gene="hypE"
                     /locus_tag="AC150V_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR3761910.1"
                     /translation="MAASARDELVTLSHGAGGKATRALVESLFADELANDALAPLGDS
                     ALVDLPAGRVAVTTDAYVVKPLRFPGGDIGELSVNGTVNDLAVAGARPLWLTAGFVLE
                     EGLPVEELRTIAASMAAAARRAGVAVAAGDTKVVERGRCDGLYVTTAGVGVVDGRAAL
                     AADRVQPGDRVLVSGTLADHGMAVMIARGELALETDVESDTAPVHELAAALLALGKRL
                     RWMRDPTRGGLATALNELVAESRLAVRLDEAALPVRRTVAAACEILGIDPLYVANEGK
                     LIAVVAPGAADEALAGLREHPLGREAALVGSVEEATEPFLLLDTTLGGSRIVDMLVGD
                     PLPRIC"
     gene            complement(4693..>5522)
                     /gene="hypD"
                     /locus_tag="AC150V_02955"
     CDS             complement(4693..>5522)
                     /gene="hypD"
                     /locus_tag="AC150V_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR3761911.1"
                     /translation="RDSLLDAKARGADVRMVYSPLDALALARKEPERHVCFFAIGFET
                     TAPATAVTLLRARAEEVRNFSVFCNHVLIEPPLRAILSTPGLRVDAFVGPGHVSTVIG
                     TDPYRFIPEEFGRQVVVSAFEPVDLLESIAMILRQRAEGRCEVENQYRRVVRAEGNAR
                     AAAAIAQTMEEREDFEWRGLGLLPRSALQLRAGFAPWDAEHLFTLPGARIEDAKACRC
                     GEVLTGQIKPWECSVFGTACTPERPLGTCMVSSEGACAAFYAYGRLRRPREGTTVGG"
BASE COUNT          863 a         1954 c         1854 g          851 t
ORIGIN      
        1 cgagtcgcgc tcgtcggtct cttcgagcag gcgcggaatc gcgagcgcgg ccacgagccc
       61 gccgtcgaca ggcacgacgg cgccgttgac gaagctggag cggctgccgc agagataggc
      121 gatcacatcg gccacctcct cgatgctgcc gatgcgtccg agcggatggg cgtggccgag
      181 caccttctgc cgctcgggtg gatgggcctc ttcgaacatg tcggtgcgga tgaaacccgg
      241 cgcgacggcg ttgacccgga ttcctcgtgg gccgagctcg acggcgaggc tctcggtcag
      301 atgctggaga cccgccttcg aggctgcgta gggcgcgagt cctgcgatcg tgcgcagacc
      361 catcaccgag ctgacgttga cgaccgcacc cgcccgggat cgctcgagcg accgaatgag
      421 cccacggctg agcacgaagg cggcgctgag gttgatctcg agaatgcggt cccacgtcgc
      481 atccgacgtt tcggcgatcg gaacgagctc tgcgacgcct gcgttgttca cgagcgcctg
      541 gatcgacgga tactccgacg cgagcgagtc ggcgagcgcg ttcgtcgcgg cgcggtcgcc
      601 gaggtcacat gctcgggcct cggtctcgat ccctcgctct cccagccgcc gggcggcgtc
      661 cgagagtcgc gcctcgtctt gtgccacgag cacgacatgc gctccctcgg cccccaacgc
      721 ctcggcgacg ccgtagccga tccccctgct cgctcccgtt acgacggcaa cccgttgcgg
      781 ctcgctcatg gtggcattca agcgcctcgc ccccgggcgg agcgaccgcg cggccgcggg
      841 gctcgcgcgg atcaggccgt catctgcgcg tgtgcctccg cctccgccac aacgccgagc
      901 gcgacgaggt cgagggggcg gatccacgca tcgcccaggg gaatgccacc cttgtccatg
      961 atcttgtcgc gcaacgggat cgcccagacg tgctcgatca gaagcactac cgcggcggtc
     1021 tcgggcggga tctcgtctac gacgtaccac tcgtcggggt cacccagcgc gtgcccgccg
     1081 cgttcggcca tcgcttcggc gccagcctcg gccccttcaa cgaagccttc ctcgccgtcc
     1141 atgccgagcc cgatcagagc accgacggtg gcgccgaact cctccgcctc ctccaggctc
     1201 aagtcgcttg cctgcaggat ctccacgtcg ccttccgcgt ccttgttgac gacgacgccg
     1261 tcgatcagtc ggatgattcc gttgtcgcgc agccgcacca gctcggccgc gatctcgcca
     1321 gtgaactccg gcttctcgaa gccgaagacg agcagctgca cgggcccaat agccatcgat
     1381 agctctccct tccggtttac gagttgagga tcttctgctt ctgggcgtcg aactcgtcct
     1441 gcgtcaagac gcccgcgtcg aggaggccct tcagcttcgt gagctcttcc accttttcgt
     1501 ccatcgaggt ggccgcagaa gcggcagcct gcggggccgc gggctgcgcc gggccagcct
     1561 gctgcgcttc gagctcagcg atccgcgcct cctgctcagc ctccctttgc tgcccctgtg
     1621 cggacttctt gccgccgtag tacgcggcgc cgccgaccat cgccgctcgg gccaccggcc
     1681 tgcgtctcct cagtggaggc atcagctgct cctttccttt tgcgctttcg gttcggctcg
     1741 attctgtcac gtggccgccg ggcctcggtg tgacaacgtc gaatcatccg ggtcgggagg
     1801 gctcgtcgcc caccatcccg agccgggtcg gaagctcggg tacgcccgcc atctccggcg
     1861 cctcctcgtg cattccctcc gaccagccgg catagcgacg ggcgagcggc gcggctgaga
     1921 ttccgtgggc gaagacgctc agcaagaccg tcaccgtcat cgtcacgacg atggtctcga
     1981 gacctgcgag gtcggactcg tccgcgacga cgatgaccgc gaggatgatc gaggccaggc
     2041 cgcgcgggcc gaaccagccg aggaagagga cggttcgcgc ctgcaagcct gtgccgacca
     2101 gcgagatcgc caccggaagc atcctcagca ccgtcagtgc cagcacggcg tacaggacgg
     2161 tcttccaggt cgtgagctcg agcagttggt aggcgacggc accgaacgcg tagaagacga
     2221 agaggttgag cagctggccg atgtcctcgg tgaagtcgac ggcggtctcc cgcacgatcg
     2281 cacctgctgc gacgccgccg acgaaggcgg cgatgaagcc gctgccgccg atctcctcgg
     2341 cgacccacca cgcggagacg gccatcgccg ccactacgag ttgccggaag acgccggtca
     2401 cccacccccg gcggaccgac ctgacgagga gcaaggcgcc taccgctcct attgccactc
     2461 cgacggccaa cccgagaccg agcttctcga ggatcagctg gcccgccgat gcagccgtga
     2521 cctcctcctc tgccaccgcg agcaccacga agatcgtgaa gagcggaacg tcgagcccgt
     2581 cgttgaggcc gctctcgacg ttcaaggatt gacggatccg caccgggagt ctcggattgc
     2641 tcacgatcga ctcaccgagc gcggcatcgg tcggggcgag cactgctgcg aggacgaacg
     2701 cctcccagaa gatgagctcg tcatcgaaca ggaggagcgc cgccacgccc ccgagcgcga
     2761 tcgtgagcgg aaggccgacg gcgagcagcc ggcccgggat ggccgcgttt ctactcacct
     2821 tccgtaggtc gatccgcgcc gcgtccgtga agagaacgag agccagcgtg atctcggcag
     2881 tggtgaggag cagctcgccg atcgtgaccc ggtcggctcc tccgccggca gcctcttcga
     2941 caacggcgcc gaaatcgacc ccgccggcgg ccgcgacgac cagcccgacg gcgacgaaga
     3001 ccatcggtgc cgtgatcgga gtgcgttcga gccggcccga gacgagcccg taggcgatga
     3061 tcagcagggc gaagaccccg atctcgatca tgcttctagc ggcaggcggt cgaggaggct
     3121 acgtctcggc ggcgcacgtc gcggcaatcc tcgcaggttc gcgcccggcc aaggtcgtca
     3181 gcgcgcgggt gattcacccg ggtgattcgg ctcggtcaat gcactggcga ggcccgctct
     3241 tgcataggct actgcggtcg accccaacga aggaggacga gtggtagcca ccagcttctt
     3301 ggacgtgctc tggacgatgt tgctcgtatt cgcctggatc gcgttcttca tgctcctttt
     3361 tcacgtcttg atcgacctgt tccgcaggca tgacgcgtcg ggctggaaaa agacggcctg
     3421 ggtggtcttc ctgatcttcg tgccgttcat cggcatcttt gcctatctga tcgtcaacgg
     3481 gcaggggatg gcagagcgca gtgcgaggca gatccaggcc cagcaagaag cggcggacga
     3541 gtacatccgc tccgtcgcgg gctcgagcgc caccgagcag atcgcgcggg cgaaggagct
     3601 gctcgaccag ggcgcgatct cgcaggagga gttcgaccac atcaagacca aggcgctcgc
     3661 ctcggcctag cgtccggctc tcagcagatg cgcggcagcg gatcgccgac gagcatgtcg
     3721 acgattctgc tgccgccgag ggtggtgtcg aggaggagga acggctcggt cgcctcctcg
     3781 acggagccca cgagggcggc ttcccgtccg agtggatgct cgcgcagccc ggccaatgcc
     3841 tcgtcggccg cgcccggcgc cacgacggcg atgagcttgc cctcgttcgc cacgtagagc
     3901 gggtcgatcc ccaggatctc gcaggcggcg gcgaccgtcc gtcgcacggg gagggcggcc
     3961 tcgtcgagcc tcacggcaag ccggctctcg gcgacgagct cgttcagggc ggttgcgaga
     4021 ccgccacgcg tcgggtcgcg catccagcgc agtcgcttgc cgagcgcgag cagcgccgcg
     4081 gcgagctcgt gtaccggggc ggtgtcgctc tcgacgtcgg tctcgagcgc cagctcgccg
     4141 cgcgcgatca tcacggccat gccgtggtcg gcgagcgttc ccgagaccag cacacgatcg
     4201 ccgggttgca cgcggtcggc ggcaagcgct gcgcgcccgt cgacgacgcc gactccggcg
     4261 gtcgtcacgt agaggccgtc gcagcggccg cgctcgacga ccttcgtgtc gcccgccgcc
     4321 accgccacac cagcccggcg ggccgcagcc gccatcgagg ccgcgatcgt ccgcagctcc
     4381 tccaccggaa ggccctcctc gagcacgaag ccggcggtca gccagagcgg ccgcgcgccg
     4441 gcgacggcca ggtcgttcac cgttccgttc accgagagct cgccgatgtc accgcccggg
     4501 aagcggagcg gcttgacgac gtaggcgtcg gtcgtgacgg cgaccctgcc ggccgggaga
     4561 tcgacgaggg cgctgtcgcc gagcggcgcg agcgcgtcgt tcgcgagctc gtccgcgaac
     4621 aggctctcca cgagcgcgcg ggtcgccttt ccgccggctc cgtgcgagag ggtgacgagc
     4681 tcgtcgcgag cgctagccgc caacagtggt tccctcgcgc ggccgcctga gccgtccgta
     4741 ggcgtagaac gcagcacagg cgccctcgct cgacaccatg caggttccga gcgggcgctc
     4801 cggagtgcac gccgtgccga agacgctgca ctcccagggc ttgatctgcc ccgtcagcac
     4861 ctcgccgcag cggcacgcct tcgcatcctc gatccgcgcg cccgggaggg tgaagaggtg
     4921 ctcggcgtcc cacggcgcga acccggcacg gagctgcagt gcgcttcgcg gcagcagccc
     4981 gaggccacgc cattcgaagt cctcccgctc ctccatcgtc tgggcgatcg ctgcggcagc
     5041 gcgcgcattc ccctccgctc gcacgacgcg ccggtactgg ttctcgacct cgcagcggcc
     5101 ctccgcccgc tggcggagga tcatcgcgat cgactcgagc aggtcgaccg gttcgaaggc
     5161 cgagacgacg acttggcggc cgaactcctc cgggatgaac cggtagggat cggtgcctat
     5221 cacggtcgac acgtggccgg gcccgacaaa ggcgtcgact cgcagacccg gggtcgagag
     5281 gatcgcgcgc aggggcggtt cgatcaggac gtggttgcag aagaccgaga agttccgcac
     5341 ctcctctgcc cgggcgcgca agagcgtgac ggcggtcgcg ggcgcagtcg tctcgaagcc
     5401 gatcgcgaag aagcagacgt ggcgctcggg ctccttacgg gccagcgcga gcgcatcgag
     5461 cggcgagtag accatgcgta cgtccgcgcc acgggccttt gcgtcgagca gtgagtcgcg
     5521 ct
//
LOCUS       DBNGNK010000104        10989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_233164,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000104 DBNGNK010000000
VERSION     DBNGNK010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_233164"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="AC150V_02960"
     CDS             complement(<1..760)
                     /locus_tag="AC150V_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3761912.1"
                     /translation="MGVATPFKVGLSPDVRGPDGNPSLDLGLRLLDEVDGVEWEFIAD
                     RGVVELEPEQVRGFDAVLLADTSVVRETLAGADRLVHVARLGVGFDDLDLEGCTERGI
                     VITNAPDGVRRPMAVSAILLVLALAHRLLEKDRLARSGRWAEQWSYLGTGVTGLTLGL
                     VGAGNIGREICRLAKPFELETVAFDPYVDDATAAEVGFRLVDLETLMSASDFVCISCP
                     LNEETRHLVDADMLALMKSNAYLVNVARGPIVDEA"
     gene            complement(762..1271)
                     /locus_tag="AC150V_02965"
     CDS             complement(762..1271)
                     /locus_tag="AC150V_02965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3761913.1"
                     /translation="MRSLMRLCSQSDFATIHSIINDAAQAYKGAIPDDRWEEPYMTLD
                     ELRQETDDGVAFWGLERDGELVGVMGVQDRGDVHLLRHAYVATGHRREGVGTELLRHV
                     ESLVSGPILVGTWAAADWAISFYEKEGYRQVSDVEKDRLLRRYWNIPERQVETSVVLA
                     NERWARAGA"
     gene            1283..2080
                     /locus_tag="AC150V_02970"
     CDS             1283..2080
                     /locus_tag="AC150V_02970"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761914.1"
                     /translation="MTIGNHEDSPEYGRLEGRRAIVTGAAGGTGRAVANRFLREGARV
                     ALVDAAADPLEELRRALTATHGDADVVAVRADVSDEAEVGGIVSAAVEAFGGLDIVVN
                     NAAVRFADRDTTVDSLERDVWERTLAVNLTGPFLVCKHAIPHLLRSPCGVIVNVSSTA
                     GIAGDPEAHAYGASKGGVISLTLGIAQRFGPDGLRAVVLCPGLVDTPMLQAALDREEL
                     TQKLVETTALRRIATPEEVASYAAFLVSDDARFVTSCVVRVDGGLVK"
     gene            complement(2073..2270)
                     /locus_tag="AC150V_02975"
     CDS             complement(2073..2270)
                     /locus_tag="AC150V_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761915.1"
                     /translation="MPWVERETAWNDLAVEYCDVTGQLLPRRYWSFEVDGKEIRTVDP
                     RYERLYIEYVRPRQASGDAVT"
     gene            complement(2257..4023)
                     /locus_tag="AC150V_02980"
     CDS             complement(2257..4023)
                     /locus_tag="AC150V_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3761916.1"
                     /translation="MRDHVWLRVLHSQLVNICEEMGLATMRTSYSPIFSEGLDFCCLI
                     LNAEGELVAMQNLNPAMTGQALFSGRWVVDDVGVDNFAPGDVVIHNDPYRGGSHMPEH
                     LVITPFFYGGELRAWLCNIAHVAEIGGMAPGSFSANATEVYQEGLRLPPVKIVDRGEP
                     VRDVWRIMLANHRTPDHSWGDFNAMIGSLHVGARRLEALFDEHGAQAIEQAVPALFDY
                     SEEWIRRDIAELPDGTYSGEDCQEDDGFESRPYFLRVDLTIAGDHMIVDWSRSDPQAR
                     GAINAPYPVTASATYTGILYVIGSGAPFNSGAIRPIDIIARPGTVVNVLHPGACVGGQ
                     TELQPRIVELIQGKVLSQVAPERTSAASGGTSGNFLFGGMHPRSGRYYTNYHFEGMGW
                     GGRATTDGNNAQIVPHGNCRNTPVEVFESRYPWFTHEYRLNDDAGGAGRTRGGLGITR
                     LLEVEADEIVVSALCDRSKVRPWGVHGGLPGDRLAYLVKPEGSRAFRTFSEAFGTVSD
                     TKFSNVRLVRGDQVKLRSPSGGGYGPPLERDPERVAEDVRNRFVSRESAEEQYGVVLD
                     GDTLDTEATRRRREEMSRDAMG"
     gene            complement(4016..6082)
                     /locus_tag="AC150V_02985"
     CDS             complement(4016..6082)
                     /locus_tag="AC150V_02985"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3761917.1"
                     /translation="MGYRIGVDTGGTFTDLVTVDESSGDVATVKVSSTPDRPAAAVLE
                     ALRRAGLSSDTVEYFALGTTIATNCLLERKGRRTLFLTTAGFEDVPYIQRVDRRTLYD
                     LQWQKPEPYVVRHDCLGVVERIRHDGSVRTPLEDSEVARVVDCVREIAGGHPDGIACA
                     VSFLFAYVNAEHERKLAEALRAALPDTPISVSSEVAPIWREYERANTVIVDAYLRRLV
                     GEFAGELDEGLAARGITCPRFLLKSNGGQVPTATADRQPVNFTLSGLAGGIIGGKHFA
                     DEVGAENVVTLDMGGTSADVGVVVDGQARSSSQYEIEWGLPIAIPVIDLTTIGAGGSS
                     LAGFDRGGFLTVGPESAGADPGPAAYGLGSERATLTDANLVLGRLNADYFLGGELPLD
                     TARAEAAVGRIAERLDVTLEDCAQAIVDVATENMANAVRLLCADRGLDYRRFDLEAFG
                     GAGPLHGALLSRRVGLGRVIVPPSPGLTSAYGAQAADLRVDRRLTRLLASDVAADGEL
                     DAAVSRIADETLAELRAEGAEREPALLVTVSSRYRGQNFELEIPVPLNGTRDVVGSVV
                     ARFHQEHERLYGYRLDGAVVEFVHLNATAIERREPLPPRHLGPGGAVEPVEVRPVFFG
                     EGGWVDTPIYRRPPLPAGATIDGPAIIEEVDSTTLVLDGQTCRIHETATLVISEHVEQ
                     EGRARA"
     gene            6130..6510
                     /locus_tag="AC150V_02990"
     CDS             6130..6510
                     /locus_tag="AC150V_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3761918.1"
                     /translation="MELIHTCYRITDPEKSIAFYEALGLEKRRELPIRDEAMNYFLGV
                     PGKPLPELELTHNFGVDSYELGSAYGHIALTVQDLDETLAKLSQQGIEPERPPYTVRD
                     GGSRLCFVRDPDSYRIELIERSDY"
     gene            6605..7741
                     /locus_tag="AC150V_02995"
     CDS             6605..7741
                     /locus_tag="AC150V_02995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3761919.1"
                     /translation="MRTEAGDDAGQGADTARPEIAARLQRLLERRVSKGDVRHVLVGV
                     ERTDGSLRWIGAVGHAAPEGPEITARQPFYVASVTKLYIAVVVLQMHEEGLLDIADPV
                     TEYLPRSSVDGLHVLDGVDWIDSVTIRHLLGHTSGLADYVEDRPAGGKSLMDQILSAG
                     DRAWTFDEMKDMVRRLEPHFPPSPTGTPRQRARYSDTNYQLLIAIIEAVAGHPLPRAF
                     EQRLLTPLDLDHTYVAGHPRADMPTCEPATVWFKDRPLEISEAMRSARDLVSTTNDSL
                     TFMAALVRGDLFQRAETSLLMSQRWNRIMFPLQYGLGMMRFRMPRLLSPTYPVPELLG
                     HSGSTGSWLFLCPKLSLLLTGTLDQASSPALPFRFLPKLLRVLR"
     gene            7993..9171
                     /locus_tag="AC150V_03000"
     CDS             7993..9171
                     /locus_tag="AC150V_03000"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3761920.1"
                     /translation="MTHAPESWLRSHLVELRRRHRLPGVAAAIVGSNGRVEIDSVGVT
                     KRDGDTPVKRDDSWHIGSCGKSMTAALYARLVERGDAAWGVGLHSLFPDLAGSMHPDW
                     ADVAVEDVLLHRAGLRNNVGRTEMIAAYSDTRPLRDQRTEATAAALSVAPRRRGSFRY
                     SNLGYIIVGAAIERITDSPFESALDTHVLQALGMTSCGFGPPPQVWGNGGRAMLARFG
                     LVVGRGAPGDPLQSESDNPPIMSPAGRLHLTLEDWAKFQRVFLAPGTDFLRPRTVERL
                     LTPAVTGAGTEWALGWANAGSLGGASFGQQGSNTYWVATALIDRTREHTAMVVCNDGR
                     TMMLKHTARFAAHLLDERRSREGEDEHVDGRRVDELELLGALRAIPGRTRLRRERSGT
                     "
     gene            9279..9512
                     /locus_tag="AC150V_03005"
     CDS             9279..9512
                     /locus_tag="AC150V_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761921.1"
                     /translation="MKSSVPALSTLVASWASLLLVFAAVALAGAPVARAEAHVAEKCK
                     RMLRGGKIVKSPGVRCVVLRRSGEVITIRPATK"
     gene            complement(9549..9881)
                     /locus_tag="AC150V_03010"
     CDS             complement(9549..9881)
                     /locus_tag="AC150V_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017679815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3761922.1"
                     /translation="MRVGWSHNAKAQIAMAEMEKPSWTFLTNHAVVLLCIDREPGIRV
                     RDMADRVGITERATHRILSELTEAGYITKTRVGRRNTYKINQRRRLRHPLADHQQVGK
                     LLAVLTDD"
     gene            10012..10425
                     /locus_tag="AC150V_03015"
     CDS             10012..10425
                     /locus_tag="AC150V_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR3761923.1"
                     /translation="MALVRWKPFQELDTFERMRRLVDDFGVAPLSLPAADVYETDGEY
                     VYEFEVPGFEEKELTVEVTDHVLKVKGERLEEKEEKEKTYRLHERMSRSFERSFELPL
                     AADTSAIGADFDKGVLRVHAAKEKEEKPRTIEITA"
     gene            10533..>10989
                     /locus_tag="AC150V_03020"
     CDS             10533..>10989
                     /locus_tag="AC150V_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3761924.1"
                     /translation="MTNGVAGHATLDELDAALETIRRSPAHVGVVELIVRRPQENERE
                     VLAEGTLDQASGLAGDGWRNGSQADDPDTQLTLMNARAIAVLTRDRERWPLAGDQLYV
                     DLDLSEENLPPGARLELGSALVEVTAVPHTGCAKFSGRFGKDALRWVSTP"
BASE COUNT         1787 a         3777 c         3669 g         1756 t
ORIGIN      
        1 cagcctcgtc gacgatcggc ccgcgcgcga cgttcacgag gtaggcattc gacttcatca
       61 gggccagcat gtcagcgtcg acgaggtggc gcgtctcttc gttcagcgga caggagatac
      121 agacgaaatc cgaggccgac atgagcgtct cgagatcgac gagccggaag ccgacctccg
      181 ccgcagtcgc gtcgtcgaca tacgggtcga acgcgaccgt ctcgagctcg aagggcttcg
      241 cgagccggca gatctcccgg ccgatgttcc cggcgccgac gaggcccagc gtgagccccg
      301 tcacgccggt tccaagatac gaccactgct ccgcccagcg gcccgagcgc gcgagccggt
      361 ccttctccag cagacgatgg gcgagcgcca gcacgagcag aatcgccgac acggccatcg
      421 gtcggcgcac cccgtcgggg gcgttcgtga tcacgatccc ccgctcggtg cacccctcga
      481 ggtcgagatc gtcgaatccg acgccgagtc gcgcaacgtg cacgaggcgg tcggcgccgg
      541 cgagtgtctc tcgcacgacc gacgtgtccg ccagcagcac cgcgtcgaag ccgcgcacct
      601 gttccggctc gagctcgacg accccccggt cggcgatgaa ctcccactcc acgccgtcga
      661 cttcgtcgag caggcgcagg ccgaggtcga gcgacgggtt cccgtcgggc ccgcgcacgt
      721 cgggcgagag cccgactttg aagggcgtgg cgacgcccac gtcaggcgcc ggcccgcgcc
      781 cagcgctcgt tcgcgagcac cacggacgtc tccacctgac gctcagggat gttccagtac
      841 ctccgcagca ggcgatcctt ctcgacgtcc gacacctggc ggtatccctc cttctcgtag
      901 aacgagatcg cccagtcggc ggcggcccac gtgccgacca ggatcgggcc ggacacgagt
      961 gattcgacgt gccgcagcag ctcggtgccg accccctcgc ggcggtgccc ggttgccaca
     1021 tacgcgtgcc ggagcaaatg cacgtctccc cgatcctgca cgcccatgac gccgaccagc
     1081 tcgccgtctc gctccagacc ccagaaggcc acgccgtcgt ccgtctcctg cctcagctcg
     1141 tccagcgtca tgtacggctc ttcccagcgg tcgtctggaa tggcgccttt gtaggcttgc
     1201 gctgcgtcgt tgatgatcga atggatcgtc gcgaagtcgg actgcgagca gagacgcatc
     1261 agggagcgca cggagtgagg cggtgacgat cggaaaccac gaggacagcc ccgagtatgg
     1321 tcgactcgag gggcggcggg caatcgtcac cggggccgcg ggcggcacgg gccgcgcagt
     1381 cgcgaaccgc ttcctgcgcg agggagcgcg tgtcgcgctc gtcgacgccg cggcggatcc
     1441 gctcgaggag ctccgacgcg ccctcaccgc gacgcacggg gacgccgatg tcgtcgccgt
     1501 gcgcgccgat gtgagcgacg aggccgaagt gggcggaatc gtgtcggccg ccgtcgaggc
     1561 cttcggaggg ctcgacatcg tcgtcaacaa cgcggccgtc cgcttcgccg accgggacac
     1621 gactgtcgat tcgctcgagc gagacgtctg ggagcggacg ctggcggtca acctcaccgg
     1681 ccccttcctc gtctgcaagc acgcgatccc ccaccttctg cgatcgcctt gcggcgtcat
     1741 cgtcaacgtc tcgtcgaccg cgggcatcgc cggcgatccc gaagcccacg cctacggcgc
     1801 gagcaagggc ggcgtgatct cgctcacgct cggaatcgcg cagcgcttcg gccccgacgg
     1861 gcttcgcgcc gtcgtgctct gcccggggct cgtggacacg ccgatgctgc aggccgccct
     1921 cgacagggaa gagctcacgc agaagctcgt cgagacgacg gcgctccgcc ggatcgcgac
     1981 gcccgaggag gttgcctcct atgccgcctt cctcgtctcc gacgacgctc gcttcgtcac
     2041 gtcctgcgtc gtcagggtcg acggcggcct cgtcaagtga ctgcgtcgcc cgacgcctgc
     2101 cgcgggcgca cgtactcgat gtagagccgc tcgtagcgag ggtcgacggt tcggatctcc
     2161 ttgccgtcca cctcgaagct ccagtagcgc cgcggcagca gctgacccgt cacgtcgcag
     2221 tactcgacgg cgaggtcgtt ccatgccgtc tcgcgctcaa cccatggcat cgcggctcat
     2281 ctcctcccgt cgccgccgag tcgcctccgt gtccagcgtg tcgccgtcga gcacgactcc
     2341 gtactgctcc tccgcgctct cccgggagac gaatcgattg cgcacgtcct ctgccacccg
     2401 ctcggggtcg cgctccaacg gcgggccgta gccgccgccc gacggcgagc ggagcttcac
     2461 ctggtcgccg cgtacgaggc ggacgttcga gaacttcgtg tcgctcacgg tgccgaacgc
     2521 ctccgagaac gtccgaaacg cgcgcgagcc ctccggcttg accaggtacg cgagccgatc
     2581 gcccggcagg cccccgtgca caccccacgg gcgcaccttc gagcggtcgc acagcgcgct
     2641 gacgacgatc tcgtcggcct cgacttcgag cagtcgcgtg atccccagcc cgcccctggt
     2701 gcgaccggcg ccgccagcgt cgtcgttgag tcggtattcg tgcgtgaacc acgggtagcg
     2761 cgactcgaac acctcgacag gggtgttccg gcagttgccg tgcggcacga tctgcgcgtt
     2821 gttcccgtcg gtggtcgcgc gcccgcccca ccccattccc tcgaagtggt agttcgtgta
     2881 gtagcggccg ctccgcgggt gcatgccgcc gaacaggaag tttcccgaag tgccgccgga
     2941 ggccgccgac gtgcgctccg gcgccacctg gctcaggact ttcccctgga tcagctcgac
     3001 gatccgaggc tggagctcgg tctgccctcc cacgcaggct cccgggtgga gcacgttcac
     3061 caccgtgccc ggccgggcga tgatgtcgat cggcctgatc gccccggagt tgaaaggcgc
     3121 gccgcttccg atgacgtaaa ggatccccgt gtacgtcgcc gacgccgtca cggggtacgg
     3181 ggcgttgatc gcgccgcggg cctgcgggtc ggagcggctc cagtcgacga tcatgtggtc
     3241 gccggcgatc gtcagatcga cgcgaaggaa gtaggggcgc gactcgaagc cgtcgtcctc
     3301 ctggcagtcc tcccccgagt acgtgccgtc cgggagctcc gcgatgtcgc gccggatcca
     3361 ctcctccgag tagtcgaaga gagccggaac cgcttgctcg atcgcctgcg cgccgtgctc
     3421 gtcgaacagc gcctcgagcc tcctcgcgcc gacgtgcagc gacccgatca tcgcgttgaa
     3481 gtcaccccac gagtggtcgg gcgtccgatg gtttgcgagc atgatccgcc agacgtcgcg
     3541 gacaggctcg ccgcggtcga cgatcttcac cggcggcagc cgcagcccct cctggtacac
     3601 ctcggtcgcg ttggccgaga aggagccggg ggccatcccc ccgatctcgg ccacgtgggc
     3661 gatgttgcag agccacgccc gcagctcgcc gccgtagaag aacggcgtga tgacgaggtg
     3721 ctccggcatg tgtgatccgc ctcggtaggg gtcgttgtgg atcacgacgt cgccgggcgc
     3781 gaagttgtcg acgccgacgt cgtcgacgac ccagcgcccc gagaagagcg cctggccggt
     3841 catcgccggg ttgagattct gcatcgcgac gagctcgccc tcggcgttca ggatcaagca
     3901 gcagaaatcg agcccctcgc tgaagatcgg cgagtaggac gtgcgcatcg tcgcgagccc
     3961 catctcctcg cagatgttga cgagctggga atggagcacc cggagccaga cgtgatcacg
     4021 cacgggcgcg accctcctgc tccacatgtt ccgagatcac gagggtggcg gtctcatgga
     4081 tgcgacaggt ctggccgtcg agcacgagtg tcgttgagtc gacctcctcg atgatcgcgg
     4141 gcccgtcgat ggtggcgcca gccgggagcg gcgggcggcg gtagatcggc gtgtcgaccc
     4201 agccgccctc cccgaagaag accggccgga cctcgaccgg ttcgactgcg ccgccgggcc
     4261 cgagatgcct gggcgggagg ggctcgcgcc gttcgatcgc cgtggcgttg aggtggacga
     4321 actcgacgac cgcgccgtcg agccggtagc cgtagaggcg ctcgtgctcc tggtggaagc
     4381 gtgccaccac ggacccgacc acgtcccggg tgccgttcag cggcacgggg atctcgagct
     4441 cgaagttctg gccccggtag cgcgagctga ccgtgacgag gagcgccggc tcccgttcgg
     4501 ctccttctgc ccgaagctcc gccagcgtct cgtctgcgat ccggctgaca gccgcgtcga
     4561 gctctccgtc tgctgcgaca tcggaagcga gcagccgggt cagccgacga tcgacccgga
     4621 ggtcggcggc ctgcgcgccg tacgcggagg tcaggcccgg gctgggaggc acgatcacgc
     4681 gcccgagacc gacgcgccgg gacagcagag cgccgtgcag agggccggcg ccgccgaagg
     4741 cttcgagatc gaagcgacgg tagtcgaggc cgcgatccgc acagaggagt cgaacggcgt
     4801 tcgccatgtt ttccgtcgcg acgtccacga tcgcctgcgc acagtcctcg agcgtcacgt
     4861 cgaggcgctc ggcaatacgc ccaacggcag cctccgcacg ggcggtgtcg agcggcagct
     4921 cgccgccgag gaaatagtca gcgttgagcc gcccgagcac gagattcgcg tcggtcaggg
     4981 tcgcccgctc ggagccgagc ccgtacgccg ccgggccggg gtctgcgccc gcgctctccg
     5041 ggccgaccgt gaggaagcca ccccggtcga agccagcgag tgagctccct ccggcgccga
     5101 tcgtggtgag gtcgatgacg gggatcgcga tcggcagccc ccattcgatc tcgtactgcg
     5161 agctgctccg ggcctgcccg tccacgacca cgccgacgtc ggcgctggtg ccgcccatgt
     5221 ccagcgtgac gacgttctcg gccccgacct cgtcggcgaa gtgcttgccg ccgatgatcc
     5281 ccccggccag gcccgagagc gtgaagttga cgggctggcg atcggccgtc gccgtcggca
     5341 cctggccacc gttggacttg agcaggaagc gtggacaggt gatcccgcgg gcggcgaggc
     5401 cttcgtcgag ctcacccgcg aactcgccga cgaggcgccg aaggtaggcg tcgacgatga
     5461 cggtgttcgc ccgctcgtac tcacgccaga tcggggcgac ttcgctcgac accgagatcg
     5521 gcgtgtcggg cagggccgcc cgcaatgcct ccgcgagctt ccgctcgtgc tccgcgttga
     5581 cgtacgcgaa caagaagctg acggcacagg cgatcccatc cgggtgcccg ccggcgatct
     5641 cccgcacaca gtcgaccaca cgtgccacct cggagtcctc gagcggcgtt cgcacggagc
     5701 cgtcgtgcct gattcgctcg acgacgccga ggcagtcgtg cctcacaaca tagggctcgg
     5761 gcttctgcca ctgcaggtcg tacagcgtgc ggcgatcgac gcgctggatg tacgggacgt
     5821 cctcgaagcc ggcggtcgtc agaaagagcg tgcgccggcc cttgcgctcg agcaggcagt
     5881 tcgtcgcgat cgtcgtcccg agcgcgaagt actcgacggt gtcggagctc agcccggcgc
     5941 gccgcaacgc ctccaacacc gccgccgccg gtcgatcggg cgtcgacgac accttgaccg
     6001 tggcgacgtc gccggacgac tcgtcgactg tcacgaggtc ggtgaacgtt cctcctgtat
     6061 cgacgccgat gcgataaccc atgccgtccc gccgccgagc ctaagcgatc gcgcgcctag
     6121 gatggccgta tggaactcat acacacctgt taccggatca ccgatccgga gaagagcatc
     6181 gcgttctacg aggcgctcgg cctggagaag cggcgggagc tgccgatccg cgacgaggcg
     6241 atgaactact tcctcggggt gccggggaag ccgctgccgg agctggagct gactcacaac
     6301 ttcggcgtcg actcctacga gctgggcagc gcctatgggc acatcgcgct caccgtccag
     6361 gacctcgacg agacgctggc gaagctgtcg cagcaaggga tcgagcccga gcggccgccg
     6421 tacacggtgc gtgacggagg ctcgcggctc tgcttcgtcc gagatccgga ttcgtatcga
     6481 atcgagctca tagagcgctc cgactactga gcggagatcg ggctagccag cacgcaccga
     6541 ccgggcacgg ccgtgaacac ccaacctctc cgggtatgcg gccgcagtct cgtacgcccg
     6601 catgatgcgc actgaagcgg gtgacgacgc gggccagggg gccgacacgg ctcggcccga
     6661 gatcgcagcg cgtctccagc gcctgctcga gcgtcgggtg tcaaaagggg atgtccgtca
     6721 cgtgctcgtc ggggtagagc gaacagacgg ttccctccgg tggatcgggg ccgtcgggca
     6781 cgccgctccc gagggcccgg agataacggc ccgccagcct ttctacgtgg caagcgtgac
     6841 gaaactgtac atcgccgtcg tcgtgctcca gatgcacgaa gaaggactcc tcgacatcgc
     6901 cgacccggta acggagtacc tcccgcgttc ttctgtcgac ggactccatg tcctggacgg
     6961 ggtcgactgg atcgactcgg tgaccatccg acacctcctc gggcacacct ccggtctggc
     7021 cgactacgtg gaggatcggc cggcaggcgg gaagagcctg atggatcaga tactcagcgc
     7081 aggagatcgt gcgtggacgt tcgacgagat gaaggacatg gtgcgccggc tcgagcccca
     7141 cttcccgccg tcgccgacag gcacgccccg acagagggca cgctactcgg acaccaacta
     7201 ccaactgctt atcgcgatca tcgaggcggt cgcagggcac ccgctgccga gggcattcga
     7261 gcagcggcta ctcacacctt tggatctcga ccacacatac gtcgcagggc accccagggc
     7321 ggatatgccg acatgtgagc ccgccacggt ctggttcaag gatcgcccgc tcgagatctc
     7381 agaggcgatg cgctccgcac gcgaccttgt cagcacgacg aacgattcgc tgacgttcat
     7441 ggcagcgctc gtccgcggcg atctcttcca gagggcggaa accagccttc tgatgtcgca
     7501 gcgctggaac cggatcatgt tccccctcca atacggcctg ggcatgatgc gtttccggat
     7561 gccgcggctg ctgtcgccca cgtatccggt accggaactc ctcggtcact ctggctcaac
     7621 cggctcgtgg ctcttccttt gtccgaagct cagcctcctc ctgaccggca ccctcgatca
     7681 ggcatcgagc cctgcgctgc cgtttcgatt cctccccaag ctcctccgcg tgctccggtg
     7741 aagcgagtcc cgaacctcgc tcgcccgctg cccttccggg cgtccggccg gagccaagtc
     7801 ctggcttggc ttgtgaccaa gctcgaggac caacagcaga ccgtgcgtga aaaaggctct
     7861 catcgtcagc aacccgacgg ttcagccgca tctacttaca tgatgagcga aacatgaaat
     7921 aatagttact actagcagag ctcgtcggcg acaggcccac ctcgacgctc cgacgcagga
     7981 ggatgacccc gcttgacgca cgcacccgag agctggcttc ggtctcatct ggtcgagctt
     8041 cggcggcgcc atcgtctacc cggggtcgcc gcagcgatcg ttggcagcaa cgggcgggtc
     8101 gagatcgaca gcgtcggagt gacgaagcgc gatggggaca cgccggtcaa gcgagacgat
     8161 tcgtggcaca tcggctcctg cgggaaatcg atgacggccg ccttgtacgc gcgcctcgtc
     8221 gagcgcggtg atgcagcctg gggcgttggg ctccattcac tcttccccga cttggccggc
     8281 tcaatgcatc ccgactgggc agacgtcgcg gtcgaggacg tcctcctgca tcgggccggg
     8341 ctccgcaaca acgtcggccg aaccgagatg atcgccgcgt attcggacac ccgaccgctc
     8401 cgggatcagc gaaccgaggc aacagcggcc gcgctatccg tagctccgcg ccgacgaggg
     8461 agcttccgct actccaacct cggctacatc atcgtcggag ccgcgatcga gcgcatcacc
     8521 gacagcccct tcgagtcagc actcgacact cacgtcctcc aggccctcgg aatgacttcc
     8581 tgtggcttcg gcccaccacc ccaagtgtgg gggaacgggg ggagggcgat gctcgcccgc
     8641 ttcggcctcg tcgtcggtcg cggggcgcct ggagaccccc tccagagcga atccgacaac
     8701 ccccccatca tgagccctgc tggcaggctg catttgacac tcgaggactg ggcgaagttc
     8761 caacgcgtct tcctcgcgcc cggcaccgac ttcctccggc cgcgaacagt cgaacggctc
     8821 ctcaccccgg ccgtcaccgg tgccggaacc gagtgggcgc tgggctgggc gaatgccggc
     8881 agcctcggcg gcgcgtcgtt cggacagcag gggtcgaaca cctactgggt cgcgacggcg
     8941 ctcatcgacc ggacgcgaga gcacacagcc atggtcgtct gcaacgacgg acgcacgatg
     9001 atgctcaagc acacggccag gttcgctgcc caccttctcg atgagcggcg atctcgagag
     9061 ggggaggatg agcacgtgga cgggcgacgg gtggacgagc tggagctgct gggggcactc
     9121 cgcgccatcc ccggccggac tcggctgcgc cgagagcgct cgggaacctg atttcggcgc
     9181 gtaacatgtg atttgattta caggttatag aaggcatata tagactgtct ctgtagacgt
     9241 agtcgcgtct gctggtcgag tccggaagag gtgcgcaaat gaagagctcg gttccggcgt
     9301 tgtcgaccct cgtggcgtcg tgggcgtcgc ttctgctggt gtttgccgct gtcgctcttg
     9361 cgggagcgcc cgtggctcgc gcagaagcgc acgtagcgga gaagtgcaag cggatgcttc
     9421 gaggcgggaa gatcgtgaag agccctggtg tgcgctgcgt ggtgctgcgt cgctccgggg
     9481 aggtgatcac gattcgcccc gccaccaagt aaccgcccgg aggccttccg tgcgcgaagg
     9541 tgcgctcctc agtcgtctgt gagcaccgcg aggagcttgc cgacctgctg gtgatcggcg
     9601 agtgggtggc gtagtcggcg tcgctggttg atcttgtacg tgtttcgacg accgactcgg
     9661 gtcttggtga tgtagccggc ctcggtcagc tcgctcagga ttcggtgtgt ggcgcgttcg
     9721 gtgatgccga cccggtcagc catgtcgcgc actctgatcc ccggttcgcg gtcgatgcag
     9781 agcagcacga cggcgtggtt cgtcaggaat gtccaactcg gcttctccat ctccgccatc
     9841 gcgatctgcg ccttcgcgtt gtgagaccac cctaccctca cttgtcgcct cctatcagcc
     9901 ggggaatgcc gaaagtgcga atccgtactt tcgagctggc gggctgcctc ccggtcccga
     9961 tgccgcctgt accggagaga gggagcattg gaggtgagga aaggaggtgt catggccctt
    10021 gtgcgttgga agccgttcca ggagctcgac acgttcgagc ggatgcggcg gttggtggac
    10081 gatttcggcg tcgcgccgct gtcactccct gccgctgacg tctacgagac ggacggggag
    10141 tacgtgtacg agttcgaggt tccgggcttc gaggagaagg agctcacggt cgaggtgaca
    10201 gatcacgtgc tgaaggtcaa gggcgagcgc ctcgaggaga aggaggagaa ggagaagacc
    10261 tatcgcctcc acgagcgcat gtcgcgttcc ttcgagcgca gcttcgagct gccgcttgcg
    10321 gccgatacga gcgcgatcgg cgccgacttc gacaagggcg tgctgagggt gcacgccgcg
    10381 aaggagaagg aagagaagcc gcgcacgatc gagatcaccg cgtgagacct aagcgccggg
    10441 ccgcgctatc ggggcggccc ggcgccattt ccttttgaat cgcgcccggc cgtaggctgc
    10501 tgcagcggac gacggactgt tagcttggcg agatgaccaa cggagtcgcg ggccacgcca
    10561 cactcgacga gctggatgcg gcgctcgaga cgatccgccg gtcgccggcg cacgtcggcg
    10621 tcgtcgagct gatcgtccgc cgaccgcagg agaacgagcg cgaggtgctc gcggagggca
    10681 ccctcgacca ggcctcgggc ctcgccggcg acggctggcg caacggatct caggccgacg
    10741 accccgacac ccagctcacc ctcatgaacg cacgcgcaat cgccgtgctg acgcgcgacc
    10801 gagagcgatg gccgctcgcc ggtgaccagc tctacgtcga tctcgatctg agcgaggaga
    10861 acctgccgcc cggggcgagg ctcgagctcg ggtcggcgct ggtcgaggtg accgcggtgc
    10921 cgcacaccgg ctgcgccaag ttcagcgggc ggttcggcaa ggatgccctg cgctgggtga
    10981 gcacccccg
//
LOCUS       DBNGNK010000105         3294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_518954,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000105 DBNGNK010000000
VERSION     DBNGNK010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_518954"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="AC150V_03025"
     CDS             complement(<1..967)
                     /locus_tag="AC150V_03025"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR3761925.1"
                     /translation="MHAAVVLFTRDLRLHDHPALSAAVQEAERIVPLFVLDDQILRRT
                     EAPSRIAFLLDALGDLDSSLRARGSALTIRRGDVVDETMQVVGETGAGAVFFSEDVSA
                     YALKRESLLAAAASDARIESRSFHGVTVVPPGDVTPSGGGDHFKVFTPYWNRWHVEPR
                     RAVLPAPEHLQSVSIAPRGLPALEELAPRSPAASLPAGGETPGRAQLEKWLAKGLPEY
                     GAARDDLAADGTSGLSPYLHFGCLSPLEVAERAAALPDGDAFVRQLCWRDFYHQLLAA
                     EPTYASRDMRPREDRWRDDPDALDAWRAGMTGYPLVDAGMRQLLAE"
     gene            1004..1447
                     /locus_tag="AC150V_03030"
     CDS             1004..1447
                     /locus_tag="AC150V_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761926.1"
                     /translation="MSVPSVSERDASAALAGVAAAVAWLAVEPLACRVTGSSLSTPRL
                     LGAPVADGRAGTVVGTALHLVNGAVFGIGFERLGIRGWKSGFVAAQVEGVVLWPGMAA
                     VDRFHPDRRDGTWPPLLTSGRTFAKEAIVHGVFGILLGALLKRPT"
     gene            1493..>3294
                     /locus_tag="AC150V_03035"
     CDS             1493..>3294
                     /locus_tag="AC150V_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3761927.1"
                     /translation="MTWAPVKPKVGFVRSLVHWLTAALAVLVAAWTLPGISVEGFWGA
                     LVVALVIGVLNAVLPPLIAALPIPAMAALGFIVILLLDALIIWLASEVTESAISVDSF
                     GIALLASIVIAAVTIVLQILFGTNDDDEFDMRVVRRVARRQGLLDTTDEPGIIFLEID
                     GLGYPVLQRAMRDGSAPELSRWLDEGTHALIEWETDLSSQTGASQAGILLGSNRDIPA
                     FRWIEKESGDLWAVSGPDEVVKVEERHSTGIGLLRDGGASRGNIFSGEAEAAILTVAR
                     LSGEFKANPGYRAFFANGHNVTRTLVLLVWEIILEWSAAIRQRRRKVWPAGHRGGLYP
                     FMRAGMCVVLRDLVVHGILQDMMRGRPAMYATFASYYEVSHHSGLERPDTLEALRKVD
                     QQIGRIARARRFAPRPYEIVVLSDHGQTQGATFKQRNGYGLDELVKRNVSQGSVGALL
                     GGDEHDNALGRAFGEATGRQAKDANERAKEEATEREDVIVLGSGNLGLVYLMEEQRRL
                     TLEEIEERHPKLIPALREHQHVGWLLVRSQRHGAVAIGSGGANYIDEGRVEGEDPLAL
                     FSPHAAEHLRRTDSFSNVADIMVNSFYDPMRDEG"
BASE COUNT          493 a         1107 c         1199 g          495 t
ORIGIN      
        1 cctccgcgag cagctgccgc atgcccgcgt cgacgagcgg gtagccggtc atcccggccc
       61 gccaggcgtc gagcgcgtcg gggtcgtcgc gccagcggtc ctcgcgcggc cgcatgtcgc
      121 ggctcgcgta cgtgggctcg gcagcgagca gctggtggta gaagtcgcgc cagcagagct
      181 ggcggacgaa agcgtcgccg tccggcagcg ccgcggcgcg ctccgcgacc tcgaggggcg
      241 agagacagcc gaagtggagg tagggcgaga ggcccgacgt gccgtcggcg gcgagatcgt
      301 ctcgtgccgc cccgtactcg ggaaggcctt tcgcgagcca cttttcgagt tgcgcgcgcc
      361 ccggcgtctc gccgccggcg ggcagcgagg ctgccggaga gcggggggcc agttcctcga
      421 gcgccggcag gccacgcggc gcgatgctca ccgactgcag gtgctcggga gcggggagga
      481 cggcacgccg cggttcgacg tgccagcggt tccagtacgg cgtgaacacc ttgaaatggt
      541 cgccgccgcc gctcggcgtg acgtcgcccg gcggaaccac ggtgacgccg tggaacgagc
      601 gcgactcgat ccgtgcgtcg gaagccgccg cggccagcag cgattcgcgt ttcagcgcgt
      661 aggcgctcac gtcctcgctg aagaagactg cccctgcgcc ggtctcgccg accacctgca
      721 tggtctcgtc gacgacgtcg ccccgccgga tcgtgagggc gctgccgcgc gcgcggagcg
      781 aagagtcgag gtcgcccagt gcgtcgagca agaacgcgat ccggctcggc gcctcggtgc
      841 ggcgcaggat ctggtcgtcg aggacgaaca gcggcacgat tcgctccgcc tcctgaaccg
      901 cggcggacag agcggggtgg tcgtgaagcc ggaggtcgcg cgtgaagagg acgacggcgg
      961 catgcatgcg gatattctag acaaaatctg tacaatcatg ttcgtgtccg ttccatccgt
     1021 ctccgagcga gacgccagcg cagcgttggc gggcgtggcg gccgcggtgg catggcttgc
     1081 ggtcgagcca ctcgcctgcc gcgtcacggg ctcatcgctc tcgacgccgc gcctcctcgg
     1141 cgcacccgtc gccgacggtc gggcagggac ggtcgtgggg accgcccttc acctggtaaa
     1201 cggggccgtc ttcggaatcg gcttcgagcg cctcggaatt cgcggctgga agagcgggtt
     1261 cgtcgccgcg caagtcgagg gagtcgtcct ctggcccgga atggcggctg tcgaccgctt
     1321 ccaccccgac cgccgcgacg gcacctggcc gccgctcctg accagcgggc ggacgttcgc
     1381 gaaggaggcg atcgtgcacg gcgtcttcgg aatccttctc ggcgcgttgc tgaagcggcc
     1441 gacctagagt ccaccaatcg ccccgactga gacccgaacg tacggcgagc gcgtgacctg
     1501 ggcgccggtg aagccgaagg tgggcttcgt ccgcagcctc gtccactggc tgaccgccgc
     1561 tctcgcggtg ctcgtggcgg cgtggaccct gcccggcatc agcgtcgagg gcttctgggg
     1621 cgcgctcgtg gtggcgctcg tgatcggcgt cctcaacgcc gttctgccgc ccttgattgc
     1681 tgcgctgccg atcccggcaa tggcggcgct cggtttcatc gtcatcctcc tcctcgacgc
     1741 cctgatcatc tggctggctt cagaggtcac cgagagcgcg atctccgtcg actcgttcgg
     1801 gatcgcgctg ctggcgtcga tcgtgatcgc cgcggtgacg atcgtgctgc agatcctctt
     1861 cggcacgaac gacgacgacg agttcgacat gcgcgtcgtc cgcagggttg cgcggcgcca
     1921 ggggctgctc gacacgacgg acgagccggg gatcatcttc ctcgagatcg acgggctggg
     1981 ctatccggtg ctgcaacggg cgatgcgcga cgggtcggcg ccggagctct cccgctggct
     2041 ggacgagggg acgcacgcgc tcatcgagtg ggaaacggac ctctcctccc agaccggagc
     2101 atcccaggcc gggatcctgc tcggctcgaa ccgcgacatc ccggcattca ggtggatcga
     2161 gaaggagtcg ggcgacctgt gggcggtctc gggcccggac gaagtcgtga aggtcgagga
     2221 gcggcattcg accgggatcg ggcttctccg cgacgggggc gcgagccgcg gcaacatctt
     2281 ttcgggggag gccgaggcgg cgatcttgac ggtcgcccgg ctgagcggtg agttcaaggc
     2341 gaacccgggc taccgggcgt tcttcgcgaa cggtcacaac gtcactcgga cgctcgtcct
     2401 cctcgtgtgg gagatcatcc tcgagtggtc tgccgcgatc cgccagcgcc ggcgcaaggt
     2461 gtggccggcc ggccaccgcg gcggtctcta ccctttcatg cgggcgggaa tgtgcgtcgt
     2521 cctccgcgac ctggtggtgc acgggatcct gcaggacatg atgcgcggcc gtcccgcgat
     2581 gtacgcgacc ttcgcgagct actacgaggt ctcccaccac tcggggctgg agcggcccga
     2641 cacgctcgag gccctgcgca aggtggatca gcaaatcggt cggatcgcgc gcgcccgcag
     2701 gttcgcgccg cggccgtacg agatcgtggt cctctccgac cacggccaga cccagggagc
     2761 gacgttcaag cagcgcaacg gctacggatt ggacgagctg gtgaagcgaa acgtgtcgca
     2821 gggcagcgtc ggcgcgctgc tcgggggcga cgagcacgac aacgcgctcg gccgggcgtt
     2881 cggcgaggcc accggccggc aggcgaagga cgcgaacgaa cgggcgaagg aggaggcgac
     2941 cgagcgcgag gacgtgatcg tcctcggcag cggaaacctc ggcctcgtgt acctgatgga
     3001 ggagcagcgg cggctgacgc tcgaggagat cgaggagcgc cacccgaagc tgatcccagc
     3061 gctgcgcgag caccagcacg tcggctggct gctcgtccgc tcgcagcggc acggcgctgt
     3121 cgccatcggg tcgggcgggg ccaactacat cgacgaggga agggtcgaag gcgaggatcc
     3181 gctcgcgctc ttctcgccgc acgcagccga gcacctccgc cgcaccgact ccttctccaa
     3241 cgtcgcggac atcatggtca acagcttcta cgacccgatg cgcgacgagg gctg
//
LOCUS       DBNGNK010000106         3366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1379380,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000106 DBNGNK010000000
VERSION     DBNGNK010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1379380"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="AC150V_03040"
     CDS             <1..1793
                     /locus_tag="AC150V_03040"
                     /inference="COORDINATES: protein motif:HMM:NF014585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3761928.1"
                     /translation="EDEMATTAPAIRTDPFLLEIVRNHLVTTCREMGIAMMRTSYSPM
                     FNEALDFSCVVFDGKAEMIGQAPFVPAQIGSTVHVVPTAIAEIGEDNLKPGDVLFTND
                     PYRSNCHLPEFVVVKPYFHEGQILAYTANIAHMTDVGGMVPAAFGDHQNTFQEGIRFP
                     PVKIYEEDQEVEAIFKILISNVRTPKHSYGDMKAMIGSLYLAERRIAELVERYGADTF
                     VQCCEDIKDVSERLMRAEIESWPDGEYGAEGLIEDDGVVPDRPWKIKGTLVIRGDEMI
                     VDFTGSDPQCFGPANQTFGTTASSAYNAVLHMVRSDIPYNQGCYRPISVIAPPGSFLN
                     VSYPAASVGGNSDTHPTTVDVLLKAYSQFSDRSSAADGGTCGCIGFGGEDPVTGEPYA
                     HLHLEGVGWGGRNDADGNDAQFVKNGNCPNTPVEVAETRYPFLNMAYELEPGQVGHGR
                     HRGGYGTRRIFRFLSDGVHISSHTNRHRVKAWGLDDGGDGTNSVIAFRRKGESDWVRA
                     REAFGTKSYGKFSNIVMNEGDEMLFVTPSGGGYGDPLQRDPELVLLDWDDELLDAAES
                     KDVYGVIIDESTGEVDADATAALRAERGTR"
     gene            1790..1987
                     /locus_tag="AC150V_03045"
     CDS             1790..1987
                     /locus_tag="AC150V_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761929.1"
                     /translation="MTTYKEVSELPRCGSCGKLLTTGGQIEERMGQRLVFCSEQCIRV
                     FDTYKAPKYGEQAIWPESVVE"
     gene            1984..2643
                     /locus_tag="AC150V_03050"
     CDS             1984..2643
                     /locus_tag="AC150V_03050"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3761930.1"
                     /translation="MSEAASLDTVERVSTTEHVLRSLRAAIVDGSLSQGEKLREIPLA
                     HQLGTGRSAVREALRQLLQEGLVQHEVHRGTFVRVIEPDDILDIYRAREAVETEAIDV
                     ILEREAVDLQPLRAALERLRAAAAGGEGTWLEMADVDIRFHETLVRLAGSQRLMRMYA
                     TLAAESRMLLYTYPSYSALQNVADHEQILVALERRSAEALELLREHLRFSARLALEGH
                     G"
     gene            2640..>3366
                     /locus_tag="AC150V_03055"
     CDS             2640..>3366
                     /locus_tag="AC150V_03055"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761931.1"
                     /translation="MSGRLDRKTAIVTGATEGIGDAIARAFVREGARVVLVARRPEPG
                     ERLAGELGAGAYFVAGDVADPETAERAVATAFERFDTLDVLVNNAALDLSGVPLLETT
                     REQAREVFEVNALGALWMLLECGRAMAAHGGGAIVNITSRLGLVGLPGSAVYGATKGG
                     LHALTRGAAVEWARLGIRVNAVAPGLTETPMIKTWVEAQENPEEFRRTREESIPQRRF
                     ATPEEIASAVVYLASDESSAVTGA"
BASE COUNT          551 a         1120 c         1181 g          514 t
ORIGIN      
        1 gggaggacga gatggccacg accgcacccg cgatccgcac cgatcccttc ctgctcgaga
       61 tcgtccgcaa ccacctcgtg acgacctgca gggagatggg gatcgcgatg atgcggacca
      121 gctactcgcc gatgttcaac gaggcgctcg acttctcgtg cgtcgtcttc gacggcaagg
      181 cggagatgat cgggcaggcg ccgttcgtcc cggcccagat cggctcgacg gttcacgtcg
      241 tgccgaccgc gatcgccgag atcggcgagg acaacctgaa gcccggcgac gtgctcttca
      301 cgaacgaccc gtaccgctcg aactgccatc tgcccgagtt cgtggtggtc aagccctact
      361 tccacgaggg gcagatcctc gcctacacgg cgaacatcgc ccacatgact gacgtcggcg
      421 gcatggtgcc cgcggccttt ggcgaccatc agaacacctt tcaggaggga atccgcttcc
      481 cgccggtcaa gatctacgag gaggatcagg aggtcgaggc gatcttcaag atcctgatct
      541 cgaacgttcg cacccccaag cacagctacg gcgacatgaa ggcgatgatc ggctcgctct
      601 acctcgccga gcggcggatc gccgaattgg tcgagcgcta cggcgccgac actttcgtgc
      661 agtgctgcga ggacatcaag gacgtgtccg agcggctgat gcgcgccgag atcgagtcgt
      721 ggccggacgg cgagtacggc gccgagggcc tgatcgagga cgacggcgtc gttcccgacc
      781 gaccgtggaa gatcaagggg acgctcgtga tccgcggcga cgagatgatc gtcgacttca
      841 ccggctccga cccgcagtgc ttcgggcccg cgaaccagac gttcgggacg acggcctcct
      901 ctgcctacaa cgcggtgctg cacatggtgc gctcggacat cccgtacaac cagggctgct
      961 accggccgat ctccgtgatc gcgccgccgg gctcgttcct caacgtcagc tatccggcgg
     1021 cgtcggtcgg cgggaactcc gacacccatc cgacgacggt cgacgtcctg ctcaaggcgt
     1081 actcccagtt ctcggaccgt tcctctgcgg ccgacggcgg cacgtgcggc tgcatcggct
     1141 tcggcggcga ggatcccgtc accggcgagc cgtacgcgca tctccacctc gagggcgtcg
     1201 gctggggcgg gcgcaacgac gcggacggca acgacgcgca gttcgtcaag aacggcaact
     1261 gtccgaacac gccggtcgag gtggccgaga cccgctaccc gttcctcaac atggcctacg
     1321 agctcgagcc gggccaggtc gggcacgggc gccaccgcgg cggctacggc acgcgccgga
     1381 tcttccgctt cctctccgac ggtgtgcaca tcagctcgca cacgaatcgc caccgcgtca
     1441 aggcctgggg cctggacgac ggcggtgacg gcaccaacag cgtcatcgcg ttcaggcgca
     1501 agggggagag cgactgggtg cgtgcccgcg aggccttcgg caccaagtcg tacggcaagt
     1561 tctccaacat cgtcatgaac gagggcgacg agatgctctt cgtcacccct tcgggcggcg
     1621 gctacggcga cccgctccag cgcgatcctg agctcgtcct gctcgactgg gatgacgagc
     1681 tactcgacgc ggcagagtcg aaagacgtgt acggcgtgat tatcgacgag tcgacgggcg
     1741 aggtcgacgc cgacgccact gcggctctgc gggcggagag ggggacgcga tgacgaccta
     1801 caaggaggtc agcgagctgc cccgctgcgg ctcctgcggg aagctgctca cgaccggcgg
     1861 gcagatcgag gagcgcatgg gccagaggct cgtcttctgc tcggagcagt gcatccgtgt
     1921 ctttgacacg tacaaggcgc cgaagtacgg agagcaggcg atctggcccg agtcagtcgt
     1981 ggaatgagcg aggcggcgtc actcgacacc gtcgagcggg tctcgacaac cgagcacgtc
     2041 cttcgctcgc ttcgcgccgc catcgtcgac ggctctctct cgcaggggga gaagctgcgc
     2101 gagatcccgc tggcgcacca gctcggaacc gggcggagcg cggtgcgcga agccctgcgc
     2161 cagctgttgc aggagggtct cgtccagcac gaggtgcacc gcgggacgtt cgtgcgggtg
     2221 atcgagccgg acgacatcct cgacatctac cgggcccgcg aggcggtcga gactgaggcg
     2281 atcgacgtca tcctcgagcg ggaggccgtc gacctccagc cgctccgcgc cgcgctcgaa
     2341 cggttgcgtg ccgcggccgc agggggcgag ggcacctggc tggagatggc cgacgtcgac
     2401 atccggttcc acgagactct cgtcaggctg gcgggcagcc agcgcctcat gcgcatgtac
     2461 gcgacgctcg cagcggagtc gcggatgctc ctctacacgt acccgtcgta ctcggccctg
     2521 cagaacgtgg ccgaccacga gcagatcctt gttgcgctcg agcggcgttc ggcggaggcg
     2581 ctggagctgc ttcgcgagca cctgcgcttc tctgcccgat tggcgctcga gggccacggt
     2641 tgagcggccg cctggatcgc aagacggcga tcgtcaccgg ggccacggag ggcatcgggg
     2701 acgctatcgc gcgtgccttc gtgcgcgaag gcgcgcgggt cgtattggtc gcccgccggc
     2761 cggagccagg tgagcggctg gcgggtgagc tgggcgccgg cgcgtacttc gtcgccggtg
     2821 acgtcgccga tcccgagacg gccgaacgtg cggtcgcgac cgcgttcgag cggttcgaca
     2881 cgctcgacgt cctcgtcaac aacgcggcgc tcgacctctc cggcgtgccg ctgctcgaga
     2941 cgacgcggga gcaggcgcgg gaggtcttcg aggtgaacgc gctcggcgcg ctgtggatgc
     3001 tgctcgagtg cggccgtgcg atggcggcac acggcggcgg tgcgatcgtg aacatcacgt
     3061 cgcgactagg tctcgtgggg ttgccgggat cggcggtcta cggggcgacg aagggcgggc
     3121 tgcacgcgct cacccgcggc gccgcggtcg agtgggcgcg gctcggcatc cgggtcaacg
     3181 cggtggcacc ggggctcacc gagacgccga tgatcaagac ctgggtggag gcgcaggaga
     3241 acccggagga gttccgccgg acgcgtgagg agtcgattcc ccagcgtcgc ttcgccacgc
     3301 ctgaggagat cgcctcggcc gtcgtctacc tggcctcgga cgagtcgagc gccgtgaccg
     3361 gtgcct
//
LOCUS       DBNGNK010000107         5484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1881633,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000107 DBNGNK010000000
VERSION     DBNGNK010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1881633"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="AC150V_03060"
     CDS             complement(<1..568)
                     /locus_tag="AC150V_03060"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR3761932.1"
                     /translation="MEAAPLRNSDRRPRPGSLERPINTRLVRGSALVVFVPVLLAALT
                     LGRPGPLPPPALPPSFDGDNAAALAAELATEHPDRRPGGSGSLGSLSWFAEKMAPYGL
                     ALEVDEWEEDVPDLGRVTLRNGAAVVDGDTDEAIVFVAHRDNTGVAAGASDNASGTAA
                     LVELARFFAAGGPRGEAPRPRQTLVFLST"
     gene            628..1326
                     /locus_tag="AC150V_03065"
     CDS             628..1326
                     /locus_tag="AC150V_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3761933.1"
                     /translation="MPDSSTILLVDDEDAIQTLLTYPLERDGYRVLQARDGEEAMQRF
                     GEEEVDLVVLDLMLPRVDGLEVCRRLRAESTVPIIMLTARDDELDKVLGLELGADDYI
                     TKPFSIREFRSRVKALLRRASTPHLADRREEVIDRGDVVIDIPRRTVLVRGEDVRLTF
                     VEFELLALLAENPGVVFSRRQLLEKLRGGADYREPRTIDVHVRHLREKIEPEPREPEL
                     ILTVRGAGYRFRDE"
     gene            1330..2730
                     /locus_tag="AC150V_03070"
     CDS             1330..2730
                     /locus_tag="AC150V_03070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3761934.1"
                     /translation="MPGGIRTKFALALLVLVGGSLLVAYLIVVPSLERRLVDAKLDQL
                     ARDALTVASELPEEKFLWQGTVQRGSGFFDARVVIYDVDSRFPLSLTLRADSNPVPTA
                     AIFTDPVAERAAVSRRLERGRVSRDGREYGEAALRTPGGEVVLLSASLADQLSTVKLV
                     QRRLLGATAIALGLALGLGSLAAVAHTRRIRRLERAANQIAEGQFDDAVVDRGDDELG
                     ELARAFERMRVQLAQLDMARKEFVANASHELRTPLFSLGGFLELLSDEELDDETRGGF
                     MLTMREQIDRLTKLTGDLLDLSRLDAGRIRIESDEFELTDVARTLAEELEPLAQASGH
                     VLSVQEVEPVWALADEERALQIGRALAGNALIHTPAGTRVAVRVERRVDRAVLIVEDD
                     GPGIALEHQNRIFDRFYRIEGDVASGSGLGLAIARELAERMSGELRLESESGRTVFTL
                     LLPSAPVARSPELVNA"
     gene            2784..3977
                     /locus_tag="AC150V_03075"
     CDS             2784..3977
                     /locus_tag="AC150V_03075"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3761935.1"
                     /translation="MRVGAVAALAVVAAVLGGVAALGIGSLIGVTGDGADETVFVSVP
                     RVGSGGAPTTTTNGRSAAGPGFDAAAIYTDRAQGVVTIYTVFRGSDGGGQGSGFIVDE
                     DGTILTNAHVITNVGEASPERGADAVFVEFSDGERVPAEIVGWDVFADVGVIRVDPEA
                     HSLAPIPLGDSDDVAPGNSVAAIGSPFGNTGSLAVGVVSATGRTIPSLTSVYSVADAI
                     QVDAPINRGNSGGPLLDGDGRAIGINAQIRSESGTAEGVGFAIPINIARRSLDQLAAT
                     GSVAYAYIGVRTQTVTPGIAKQFDLGAERGALIQGVESGTPASRAGLQGGGREEAYNG
                     LTVTLGGDQIVKIGDQDIASADDVSRIVTHDLLPGQRVPFVVIRDGKRETIEVTLGER
                     PRSDR"
     gene            4022..5437
                     /locus_tag="AC150V_03080"
     CDS             4022..5437
                     /locus_tag="AC150V_03080"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR3761936.1"
                     /translation="MAGRRRLLIVSNRGPVSYSRDESGDRIARRGAGGLVTALAPLVS
                     RHDVTWVAGAMTAEERAIAAAGAVEEQAADGSRYRLRLVPVDPGAYRLFYDVVANPVL
                     WFVQHGLWDLIEDPGAELSGPWHDGYVHVNDAFADVVSDELAHVPDAAVMFQDYHLYT
                     APRVVRQRHPDAAISHFVHIPWVGPERWSVLSAGIVEAIHDGLHACDVVGFHAARWRD
                     AFEASSAVYADAADTVIDVNPISVDPSEFDALSASEAVLERAEHLLSPRPPLLILRVD
                     RTDPAKNAIRGFEAFSLLLERRPDLHAQVGMLALLHPSRLSIPEYATYAEALQATATL
                     VNERFGRPGWTPITLDLRDDFLLSVAAYREYDVLLVNSVKDGLNLVAKEAPLVNARDG
                     VLALSREAGAFDELGEWVVPLDPLDVHQQSIALEQALEVAPGERRDWQEAIRAHVRAH
                     DLDAWATQQLSVLDRASTMRS"
BASE COUNT          836 a         1904 c         1916 g          828 t
ORIGIN      
        1 ccgtcgacag gaacaccaac gtctggcgcg gacggggcgc ctcaccgcgc ggcccgcccg
       61 cagcaaagaa gcgcgcgagc tccaccagtg cggccgtgcc cgacgcgttg tcgctcgccc
      121 ccgccgccac accggtgttg tcacgatggg caacaaagac gatcgcctcg tccgtgtctc
      181 cgtcaaccac cgcggcgccg ttgcgaaggg tgacacggcc gagatcggga acgtcctctt
      241 cccattcgtc gacctccagc gccaacccgt acggcgccat cttctctgcg aaccagctca
      301 aggagccgag cgagccggac ccgccgggtc gccgatccgg gtgctcggtt gcgagctcgg
      361 ccgcgagcgc ggccgcattg tcgccgtcga aggacggggg gagcgccggc ggcgggagcg
      421 gccccggccg accgagcgtg agcgcggcca ggagcacggg gacgaagacg acgagggccg
      481 agccgcgcac cagccgcgtg ttgatcggac gctcgaggga gcccggtcgc ggcctccgat
      541 cggagttgcg cagcggagcc gcctccatac cgcgggctat agtaggcggt ggcaggcgag
      601 cgcgcgctcg gcgcgaaaga cgcaacaatg ccggattcct caaccattct tctcgtcgac
      661 gacgaagacg cgatccagac gctcctgacc tatccgctgg agcgtgatgg ctatcgggtg
      721 ctgcaggcgc gggacggcga ggaggcaatg cagcgcttcg gcgaggagga ggtcgacctg
      781 gtcgtgctcg acctgatgct gccgagggtc gacgggctgg aggtgtgccg ccgcctgcga
      841 gccgagagca ccgtgccgat catcatgctg acggcacgcg acgacgagct cgacaaggtg
      901 ctcggccttg agctgggcgc ggacgactac atcacgaagc cgttctcgat ccgcgagttc
      961 cgcagccgcg tgaaagcgct gctgcggcgt gcctcgacgc cgcatctcgc cgaccgtcgc
     1021 gaggaggtga tcgatcgagg cgacgtcgtc atcgacatcc cgcgtcgtac cgtgctcgtc
     1081 cgcggggagg acgttcggct gaccttcgtc gagttcgagc tcttggcgct gctcgccgag
     1141 aaccccggcg tcgtcttctc gcgccggcag ctgctcgaga agctccgggg gggagccgac
     1201 taccgcgagc cgcgcacgat cgacgtccac gtgcggcatc tgcgcgaaaa gatcgagccc
     1261 gagccacgcg agcccgagct catcctcacc gtccgggggg ccggctaccg cttccgcgac
     1321 gagtagacga tgccgggcgg gatccgcacc aagttcgcgc tcgcgctgct cgtcctcgtc
     1381 ggcggctcgc tgctcgtggc ctacctgatc gtcgtgccat cgctcgaacg ccggctcgtc
     1441 gacgccaagc tggatcagct cgcccgggac gcacttacgg tcgcctcgga gctgccggaa
     1501 gagaagttcc tctggcaagg cacggtgcag agaggctccg ggttcttcga cgcgcgggtc
     1561 gtcatctacg acgtcgacag ccgcttcccg ctctctctca cgctacgggc cgattcgaac
     1621 ccggttccca cggccgcgat cttcaccgac ccagttgccg agcgggcagc ggtcagccgt
     1681 cgtctcgagc gaggtcgggt gagccgcgac gggcgagagt acggggaggc agctcttcgg
     1741 acgccggggg gcgaagtcgt gctgctctcg gcctcactcg ccgaccagct ctcgaccgtg
     1801 aagctcgtgc agcgccgcct gctcggcgcc acggcgatcg cgcttgggct cgccctgggc
     1861 ctcgggagcc tggcggctgt cgcgcacacg aggcgcatcc ggcgactcga gcgtgcggcg
     1921 aaccagatcg ccgaggggca attcgacgac gccgtggtcg atcgaggtga cgacgagctc
     1981 ggcgagctcg cccgtgcgtt cgagcgaatg cgggttcagc tcgcgcagct cgacatggcg
     2041 cgcaaggagt tcgtcgcgaa cgcctcgcac gagctacgca cccctctgtt ctcgctcggc
     2101 ggcttcctcg agctgctctc ggacgaggag ctcgacgacg agacccgcgg gggcttcatg
     2161 ctgaccatgc gggagcagat cgaccggctg acgaagctca ccggcgacct gctcgatctt
     2221 tcgcgcctcg acgccgggcg gattcggatc gagagcgacg agttcgagct gacagatgtc
     2281 gcccgcacgc tggccgagga gctcgagccg ctcgctcagg cgagtggcca cgtgctgtcg
     2341 gtgcaagaag tcgagccggt gtgggcgctg gccgacgagg agcgggcgct tcaaatcggc
     2401 cgtgccctgg cgggcaacgc cctgatccac acgccggcag gcacacgcgt cgccgtgcgc
     2461 gtcgagcgcc gtgtcgacag agcggtgctc atcgtcgagg acgatgggcc gggaatcgcg
     2521 ctcgagcacc agaacaggat cttcgaccgc ttctaccgca tcgagggtga cgtcgcgtcc
     2581 ggcagtgggc tgggtctcgc aatcgcccgc gagctggctg agcgcatgag cggcgagctc
     2641 cggctcgagt ccgagtcggg ccgaaccgtg ttcacgctgc tgctgccctc ggcgcccgtc
     2701 gcgcggtcac cggagctcgt caacgcctga cccttttcac gtgaaaaggc cgtggccccc
     2761 agggacgcgg ctacagtgaa cacgtgcgcg ttggagccgt cgcggcgctc gctgtcgtgg
     2821 ccgccgttct cggcggtgtg gcggcgctcg gaatcggctc cctgatcggc gtgaccgggg
     2881 acggcgccga cgagacggtc ttcgtgtcgg ttccacgcgt cggctccggc ggagcgccga
     2941 cgacgacgac caatgggcgc agcgccgccg gcccgggctt cgatgctgcc gccatctaca
     3001 ccgatcgggc gcaaggcgtg gtgaccatct acaccgtctt ccgtggctcg gacggcggcg
     3061 ggcagggttc gggtttcatc gtcgacgaag acgggacgat cctgacgaac gcgcacgtga
     3121 tcacgaacgt cggcgaagcg tcacccgaac gcggcgccga cgccgtgttc gtcgagttca
     3181 gcgacggcga gcgggtaccc gcggagatcg tcggctggga cgtgttcgcc gacgtcggcg
     3241 tgatcagggt cgatccagaa gcgcattcgc tggcgcccat cccactgggc gactcggacg
     3301 acgtggcgcc ggggaactcg gtcgctgcga tcggcagccc gttcggcaac accggctcgc
     3361 tcgccgtcgg ggtagtctcg gcgaccggcc gcacgatccc gtctctgacg tccgtgtact
     3421 ccgtcgcgga cgcgattcag gtcgacgctc cgatcaaccg cgggaactcc ggcgggccgc
     3481 tcctcgacgg cgacggccgc gcgatcggca tcaacgcgca gattcgctcc gagtcgggca
     3541 cagcggaggg cgtgggcttc gccatcccga tcaacatcgc gcgacgctcg ctcgaccaac
     3601 tcgcggcgac cggcagtgtc gcgtacgcct atatcggcgt gagaacccag accgtcacac
     3661 ccgggatcgc aaagcagttc gatctcggag ccgagcgggg cgctctgatc cagggggtcg
     3721 agtccggcac gccagcctcg cgggcgggac tccagggcgg cggccgcgag gaggcgtaca
     3781 acggcctgac cgtgacgttg ggaggcgacc agatcgtgaa aatcggcgac caagacatcg
     3841 cgagcgccga cgacgtctcg cggatcgtta cgcacgacct cctgcccggc cagcgcgtcc
     3901 ccttcgtcgt catccgcgac ggcaagcggg agacgatcga ggtcacgctc ggcgagcgac
     3961 cgcgcagcga ccgctagatg gcctgcgggc aggggctttc cagcacggag aacgcaggag
     4021 catggccggc cggcgaaggc tgctgatcgt ctcgaaccgc gggccggtct cgtattcgcg
     4081 ggacgagagc ggcgaccgca tcgcgcgccg cggtgccggc ggtctcgtca cggcgctcgc
     4141 gccgctcgta tcgcgacacg acgtgacctg ggtcgccggc gcgatgacag ccgaggaacg
     4201 agcgatcgcc gccgcaggcg ccgtggagga gcaggcggca gacggctcgc gctaccggct
     4261 acggttggtt ccggtcgatc ccggcgcgta ccgcctcttc tacgacgtcg tcgccaaccc
     4321 ggtgctgtgg ttcgtccagc acgggctgtg ggacttgatc gaagatccgg gcgccgagct
     4381 cagcgggccc tggcacgacg ggtacgtgca cgtcaacgac gccttcgccg acgtcgtctc
     4441 cgacgagctc gcccacgtgc ccgacgccgc cgtgatgttc caggactacc acctctacac
     4501 ggcgccgcgc gtcgtgcgac agcgacaccc ggacgccgcg atctcccact tcgtccacat
     4561 tccctgggtc ggcccggagc gatggagcgt gctctccgct ggcatcgtcg aggcgattca
     4621 cgacggcctg cacgcctgtg acgtcgtggg cttccatgct gcgcgctggc gcgacgcgtt
     4681 cgaggcgtca tccgccgtct acgccgatgc ggccgacacc gtgatcgacg tcaacccgat
     4741 ctcggtcgat ccgtccgagt tcgacgcgct ctcggcgagc gaggccgtac tcgagcgtgc
     4801 ggaacacctt ctttctccgc ggccgcccct gctgatcctg cgggtggatc gcaccgatcc
     4861 ggccaagaat gcgatccgcg gcttcgaggc gttctcgctg ctgctcgagc gacggcctga
     4921 tcttcacgcg caggtcggga tgctggcgct gttgcacccc tcgcggctct cgatccccga
     4981 gtacgcaacg tacgccgagg cgctgcaggc gaccgccacg ctcgtgaacg agcggttcgg
     5041 ccgccccggc tggacaccga tcacgctcga cctccgggac gacttcctgc tgtcggtcgc
     5101 cgcctaccgc gagtacgacg tcctgctcgt gaactccgtg aaggacggtc tcaacctcgt
     5161 cgcgaaggag gccccgctcg tcaacgctcg cgacggcgtc ctggcgctgt cccgcgaagc
     5221 gggcgccttc gacgagttgg gtgaatgggt cgtaccgctc gacccactgg acgtccacca
     5281 gcagtcgatc gcgctcgagc aggcgctcga ggtggcgccg ggagaacggc gggactggca
     5341 ggaggcgatt cgggctcatg tgcgcgcgca cgatctcgac gcatgggcca cgcagcagct
     5401 ctccgtgctc gatcgggcca gtacgatgcg gtcgtgaccg cggcgccgca cgtcgtcggc
     5461 ggggagctca acccgcggaa cccg
//
LOCUS       DBNGNK010000108         5228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1809943,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000108 DBNGNK010000000
VERSION     DBNGNK010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1809943"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="AC150V_03085"
     CDS             complement(<1..666)
                     /locus_tag="AC150V_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR3761937.1"
                     /translation="MILLLTASEEGRAALPGQNGKISFNSDRDGNTNLYTVNPDGSGL
                     APLTSNPAFDANATWSPDGSSLVFESHRDSPGSSDLYRMHADGTGVTRLTTQAASDAQ
                     PSWSPDGRRIAFQTDRDGNVEIYAMLADGTLPSNLTANAAADTDPSWSPAGDRIAFTS
                     QRDGNPEVYVMNADGSGQARLTADPSHDADVDWSPDAGRLAFTSDRDGDFEIFTMNID
                     GSGL"
     gene            complement(882..1595)
                     /locus_tag="AC150V_03090"
     CDS             complement(882..1595)
                     /locus_tag="AC150V_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009851510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR3761938.1"
                     /translation="MARTVGAAAGGVNPLSPQLFAFLRELAANNDRAWFTANKARYVD
                     EVQEPALAFIATFGERLHEISPHFVADPRVVGGSLFRIHRDVRFSADKTPYKTHTGIQ
                     FRHERGKDAHAPGFYLHLEPGRVFAAAGLWRPDREALRQVRDAIAADPGAWSRAVDDA
                     AASASFRLQGESLVRPPAGFDPEHPAIADLKRKDFVLVAGIGERAVGSRRFPQRLAET
                     FGAATPVVRFICSATGLPF"
     gene            complement(1718..4858)
                     /locus_tag="AC150V_03095"
     CDS             complement(1718..4858)
                     /locus_tag="AC150V_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3761939.1"
                     /translation="MNGATCHACAQPVTEGHRFCGHCGAALAEDAPVDRGEHRLVTVL
                     FCDLVDSTGIAERLGDEALHRTLDRFFAIAVDEVERYGGTVDKFLGDGILALIGAPQA
                     YEDHARRAVLASLGLRQRLHDAWPVDEMGPPPLARMGIDSGVLVVGTLGVGRRVDHTA
                     IGDTANVAARLQAAAEPGEILISERTAQRVAGYARLAPRGRLALRGRTEPVVAHRLVG
                     VGSRRSPLEGVTDRGLTPFVGRADELDVLRDAVGAIQECKGRVVSIGGAPGLGKSRLV
                     LEFRRALGAEPVTFLEGRCLSFGAATPYLPLADIVRANAGITPDDPPEALAGKVDLAL
                     SEIGVDSNRSALFLLHLLGVPAGDPIPRLSPEAVRDNVFATLREMCLEGSRRRPLVLA
                     VEDLHWIDATSEAFLRSLAEILLDARILLLATHRPEYRPPWADLAWSSSVTLGPLGTA
                     ESRRVVRAVIGPATADTPLEGAILGRADGNPFFLEELARVAGDVANPWDPAEVPDTVQ
                     GVLMARMDRLDPEAKRTLQTASVLGREFSARLLGSVWRGRGEVDHHLAKLRRLEFVFE
                     RPTVAGRRWAFTHALTQDVAYGSLLSDRRRELHRAAAESLRSMPDEQPEGVEELLALH
                     CSRAEMHDEAVRHLRAVAERSAGRYAHDEATQALELALRHVERLPRDVRDGQSVELSL
                     RLAESLYFLGQVRSSAGLLARLEEGARRRGDRNQLAELRFWIAHAHSHLGDPDEAAAA
                     ARDSAELAERAGETAVLGRARYVLAREGWWTGRWRTGISEGEAAVPILEEAGQAWWLG
                     HCLCYVGHGYQSLGQFDRALAAVSRAQEIGEATGDPRIRSFAAWSRGWYLASMGRPEE
                     GIPACEEAVAVSPDAANSAWALGFLGFAHRESGDTPRAIDELERALDVVRQTEHPRVL
                     CWFQGWLADAHLAAGRPHPARAAARSALTIAGRVQCPWAAAYATRSLGRIALLEGDTS
                     EAARHLGAAITAYHRLSADFEQALTLLDAARWHRRLGDHESATARARDASRLLAPMPV
                     PLWQQRAAQEAEPAPA"
BASE COUNT          752 a         1870 c         1882 g          724 t
ORIGIN      
        1 caggcccgag ccgtcgatgt tcatcgtgaa gatctcgaag tcgccgtcac ggtcgctcgt
       61 gaaggcgagc cgcccagcgt ccgggctcca gtcgacgtcg gcatcgtggg acggatcggc
      121 cgtcagcctc gcctgtccgc tcccgtcggc gttcatcacg tacacctccg ggttgccgtc
      181 ccgctggctg gtgaaggcga tccgatcgcc ggcaggagac caggacgggt cggtgtcggc
      241 cgcggcgttc gccgtgaggt tcgacgggag ggttccgtcc gcgagcatcg cgtagatctc
      301 gacgtttccg tcgcggtccg tctggaaggc gatcctccgc ccgtcgggcg accagctggg
      361 ttgagcgtcg ctcgccgcct gtgtcgtgag gcgggtgacc ccggttccgt cggcgtgcat
      421 gcggtagagg tcagatgagc cgggtgagtc gcggtgggac tcgaagacca gcgacgagcc
      481 gtcgggcgac caggtcgcgt tggcgtcgaa cgccgggttg gaggtcagcg gcgccaggcc
      541 cgagccgtcg gggttgaccg tgtagaggtt ggtgttgccg tcgcggtccg agttgaacga
      601 gatcttcccg ttctggccgg ggagggcggc cctcccctcc tcgctcgccg tgaggagcag
      661 gatcacgagc gcagccgcgc agacgcctct gagaatcgcc gcgaacccac tcctccacgg
      721 attcgccgac ccggccccgc gctgtcccct cgtctggacg ggcctgcgca ccagcgcctc
      781 tccgcgtccc tcatgtcgtc gacgccaggc tatttccgta cgtcgggcgc ccgcaagccg
      841 atcggccccc ccggctcgcc gcctccggtc cgatccggtc gctagaaggg cagcccggtc
      901 gcggagcaga tgaagcggac cacgggcgtc gccgctccga acgtctcggc gagccgctgc
      961 gggaaccggc gggagccgac ggcccgctcg ccgatgccgg ccacgagcac gaagtccttc
     1021 cgcttgaggt cggcgatggc ggggtgctcg ggatcgaagc ccgccggggg ccgtacgagc
     1081 gactcgccct gcaggcggaa cgaggcgctc gccgcggcat catcaacggc ccggctccac
     1141 gcgcccggat ccgcggcgat ggcgtcgcgg acctgccgga gagcttcgcg atctggcctc
     1201 cagagccccg cagcggcgaa gacgcggccc ggctcgaggt ggagatagaa gcccggcgcg
     1261 tgcgcgtcct tgccccgctc gtgccggaac tggatcccgg tgtgggtctt gtacggggtc
     1321 ttgtcggcgc tgaagcggac gtcgcgatgg atgcggaaca gcgatccgcc gacgacgcgc
     1381 ggatcggcga cgaagtgcgg gctgatctcg tgcaggcgct cgccgaacgt cgcgatgaac
     1441 gcgagcgccg gctcctggac ctcgtccacg taccgcgcct tgttcgccgt gaaccaggcg
     1501 cggtcgttgt tcgcggcgag ctcccgcagg aacgcgaaga gctggggcga cagcgggttc
     1561 accccgccgg ccgccgcgcc gaccgtccgg gccatgtccg tcctccctgt gagctgatcc
     1621 gtcgacggta cgaccctgta gggcgtcccg tctcgtcgcg cgggccggtg agttcgggac
     1681 acggcgatta cctcatcgag tcctatcccg ccgcggttca tgccggcgcg ggctctgcct
     1741 cctgcgccgc tcgctgctgc cagaggggga ccggcatggg cgcgagaagg cggctcgcat
     1801 cccgcgcgcg ggcggtcgct gactcgtggt ctccaagccg gcggtgccag cgggctgcgt
     1861 cgagcagcgt cagggcttgc tcgaagtccg cactcagccg gtggtaggcc gtgatcgccg
     1921 ccccgaggtg acgggcggcc tcgctggtgt cgccctcgag cagggcgatg cgaccgaggc
     1981 tccgtgtcgc gtacgccgcc gcccacgggc actggacccg cccggcgatg gtcagagcgg
     2041 atcgagcagc cgcgcgtgcc ggatgaggcc gccccgcggc gaggtgagcg tcggcgagcc
     2101 agccctggaa ccaacacaac acgcgcgggt gctcagtctg cctgacgacg tcgagcgcgc
     2161 gctccaactc gtcaatggcc cgcggcgtgt caccactctc gcggtgcgcg aagccgagaa
     2221 acccaagcgc ccacgctgag ttggccgcat ctggcgagac cgcgaccgcc tcctcgcagg
     2281 ccggtatgcc ctcctccggc cggcccatcg aagcgaggta ccagccgcgg ctccacgcgg
     2341 cgaagctgcg gatgcgcgga tcgccagtcg cttcgccgat ctcctgcgcc cgagaaacgg
     2401 ccgccagcgc ccggtcgaac tgcccgaggc tctggtagcc gtgcccgacg tagcagaggc
     2461 aatgcccgag ccaccacgcc tgcccggcct cctccaggat cggcacagcc gcctcgcctt
     2521 cgctgatgcc cgtgcgccag cgaccggtcc accacccttc gcgcgcgagc acgtagcggg
     2581 cccggcccag cacggccgtc tcgcctgccc gctcggccaa ctcggcggag tcgcgggcgg
     2641 cggcggcggc ttcgtcggga tcgccgagat ggctgtgagc gtgggcgatc cagaagcgca
     2701 gctcggcaag ctgattcctg tcgccgcgtc gtcgtgcgcc ttcttccagc cgcgcgagga
     2761 ggcctgcgct ggagcgcacc tgcccgagga agtagaggga ttctgcgagc cggagcgaga
     2821 gctccaccga ctggccgtcg cgcacgtcgc gcggaagcct ctcgacatgc cggagcgcaa
     2881 gctcgagtgc ctgggtggcc tcgtcatgcg cgtagcggcc ggcgctccgc tcggccacgg
     2941 cgcgcaggtg ccgtactgcc tcgtcgtgca tctcagcacg agagcagtga agagcaagga
     3001 gctcctccac cccctcgggc tgctcgtcgg gcatcgacct caggctctcc gcagccgccc
     3061 ggtgcagctc gcgacgacga tccgagagga ggctgccgta ggcgacgtcc tgggtgaggg
     3121 cgtgcgtgaa cgcccaccgc ctcccggcga cggtcggccg ctcgaacacg aactcgagac
     3181 gacgcagctt cgcaaggtgg tggtcgacct ccccacgccc gcgccagacg gaaccgagga
     3241 gccgggcgga gaactcgcgg ccgaggacgg aggccgtttg gagcgtccgc ttggcctcgg
     3301 ggtccagccg gtccatacgg gccatcagca cgccctgcac ggtgtcggga acctcggccg
     3361 gatcccaggg gttcgcgacg tccccggcta cgcgcgcgag ttcctcgagg aagaaggggt
     3421 tgccgtcggc gcgcccgagg atcgccccct ccagcggcgt atcggcggtt gccggaccga
     3481 tgactgcgcg cacgacgcgg cggctctcgg cggtaccgag ggggccgagc gtgaccgaac
     3541 tgctccacgc cagatcggcc cacggcggcc ggtattcggg acggtgggtg gcgagcagca
     3601 ggatgcgcgc gtcgaggagg atctcggcga gagaccggag aaacgcctcg gacgtcgcgt
     3661 cgatccagtg caagtcctcg accgcgagca cgagcggacg ccgtcgactg ccctccaggc
     3721 acatctcccg tagcgtcgcg aagacgttgt cgcgcaccgc ctcggggctg aggcggggta
     3781 tcgggtcccc ggcggggaca cccagtaggt ggaggaggaa gagcgccgag cggttcgagt
     3841 cgacgccgat ctcggacagt gcaaggtcca ccttgcccgc caacgcctcc gggggatcgt
     3901 cgggcgtgat gcccgcgttg gcgcggacga tgtccgcgag cggcagatac ggggtcgcgg
     3961 cgccgaagga caggcagcgg ccttcgagga atgtgaccgg ctccgccccg agcgcgcggc
     4021 ggaactccag aacgaggcgc gacttgccga gaccgggcgc tccaccgatg ctgacgaccc
     4081 tgcccttgca ctcctggatc gcaccaacgg cgtcgcggag tacatcgagc tcgtccgcgc
     4141 gcccgacgaa gggggtgagc ccccggtcgg tcacgccttc caggggcgac cggcgcgatc
     4201 cgaccccgac gagccggtgc gccacgacgg gctccgtcct ccccctcagc gcgagccgcc
     4261 cacggggcgc gaggcgcgcg taccccgcga cgcgctgcgc cgtccgctcg ctgatgagga
     4321 tctcgccggg ctccgccgcg gcttggagcc gggcggcgac gttcgcggtg tcgccgatgg
     4381 cggtgtggtc cacgcgccgc ccgacaccga gggtccccac gacgaggacc ccgctgtcga
     4441 tgcccatccg cgcgaggggg ggcgggccca tctcatcgac cggccaggcg tcgtggagtc
     4501 gctgacgcaa cccgagggac gccagcacgg cccgccgcgc atggtcctcg tacgcctgcg
     4561 gtgcgccgat gagcgcgagg atgccgtcac ccaggaattt gtccaccgtc cctccgtagc
     4621 gctcgacctc atcgacggcg atcgcgaaga agcggtcgag cgtccggtgg agcgcctcgt
     4681 cgccgagacg ctcggcgatc cccgtcgagt cgacgaggtc gcagaacaga acggtgacga
     4741 gacggtgctc accgcggtcg acgggagcgt cctcggcgag ggccgcgccg cagtgcccgc
     4801 agaaccgatg cccctccgtg acgggctgcg cgcacgcgtg acaggtggcg ccgttcatcg
     4861 gtcccctcgg atccaggcta accgatcgcg aggaggcagt cgcccgcgcc gactggtcta
     4921 tcgccgacca tcgacggatc agccccggct acgcacgggg tcgccgggca caccgtgacg
     4981 agccatgacg atccggcggt cgacctcaaa gggcgccgag ggtgacgggg aggtgtaacc
     5041 aggctgtccg tcggggcagg cagaccaatc ctccatcgaa cccgccgttg cagggggact
     5101 cgaaaccctg atgtgccgcc gcgcggcgcg tgaccacggt cgggctcccc cgaccatccg
     5161 cgctgaggag cgcgagggct cacccttccg gagcgatcct caggacgacc tgcgtgtcac
     5221 ccccgacg
//
LOCUS       DBNGNK010000109         3010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1164630,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000109 DBNGNK010000000
VERSION     DBNGNK010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1164630"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..94
                     /locus_tag="AC150V_03100"
                     /pseudo
     CDS             26..94
                     /locus_tag="AC150V_03100"
                     /inference="COORDINATES: protein motif:HMM:NF016051.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(230..550)
                     /locus_tag="AC150V_03105"
                     /pseudo
     CDS             complement(230..550)
                     /locus_tag="AC150V_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
     gene            complement(622..858)
                     /gene="rpoZ"
                     /locus_tag="AC150V_03110"
     CDS             complement(622..858)
                     /gene="rpoZ"
                     /locus_tag="AC150V_03110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR3761940.1"
                     /translation="MIEPRIDDLLEHVDSRYALVIVAAKRARQINSYHHQLGEGMGFE
                     HAPPPLIQSRSKNYLTMSMEEVSQGKIKYEYPST"
     gene            complement(855..1418)
                     /gene="gmk"
                     /locus_tag="AC150V_03115"
     CDS             complement(855..1418)
                     /gene="gmk"
                     /locus_tag="AC150V_03115"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF012832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR3761941.1"
                     /translation="MITGPSGAGKGTLIKALVERVPALEVAVSATTRAQRPGEVEGRD
                     YWFFDEAEFVRQIESGAFLEHVAYISGKRYGTLRSELDRIANDGRICVLELELEGALR
                     VQEEVPGAVTIFVAADVPELERRLTERATESTGEIGERIALARLQLEQAHQFRYMVRN
                     DDVGRAIEVLTAIVERELELAGTMSRP"
     gene            complement(1442..1771)
                     /gene="mihF"
                     /locus_tag="AC150V_03120"
     CDS             complement(1442..1771)
                     /gene="mihF"
                     /locus_tag="AC150V_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HIR3761942.1"
                     /translation="MATSKTQTQAPVRSLDQRMEALKRANDIRVRRAKLKKDLKDGRA
                     QIETILLDPPDYVSTAKVFDMLMAVPKFGRVKAARLLNQCRISQSKTVGGMSERQRTE
                     LVSLFNR"
     gene            complement(1806..2714)
                     /locus_tag="AC150V_03125"
     CDS             complement(1806..2714)
                     /locus_tag="AC150V_03125"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR3761943.1"
                     /translation="MSVTLLNASGCLDALAAPEVARSLDAFVTKTITPSPRHGNPPVR
                     IAETEYGMLNSIGLQGPGIDTFVADVLPTLVELGPALWVSVGGFSAADYADQCERLDD
                     CERVAVIELNLSCPNVDEAPETAAELVAAARRSTSKRLFAKLSPASWDVAEAARSVAA
                     AGADGLSLVNTIRGLALDPETLRPVLARATGGYSGPALKPIALACVYACASAVDLPIV
                     GMGGIRTGTDVLEFVAAGASAVALGTVLFGDPDAPSRIRSELLAAVDAHGLPDLQSAH
                     RTAHRGVQNPPHPVEKSLEIGENGPA"
     gene            complement(2711..>3010)
                     /locus_tag="AC150V_03130"
     CDS             complement(2711..>3010)
                     /locus_tag="AC150V_03130"
                     /inference="COORDINATES: protein motif:HMM:NF021903.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIR3761944.1"
                     /translation="FRTDEQAAAAELLPFAEVVLEPTLVTDALPPDPGDVLACGPEPM
                     LAALRRLVPSAQLAWEAPMACGYGACYGCVVTVEGHYERLCVTGPVLRGHPERFA"
BASE COUNT          465 a         1033 c         1036 g          476 t
ORIGIN      
        1 gcgcgccggc gccgacgtga tcgtcatggc ggccgccgtc gccgactacc ggccggcggc
       61 tgcgcaggcc gggaagcgtc cgaaggatgg agagctgtgg aacgtcgacc tcgagccgac
      121 ggccgacgtc ctgcacgctc tcggcgccga ccacgtgggc cccgcgcgcg acgaggagcg
      181 ccacgttcgc ctgtgtcgcc gggtgctccc acatacgcgt gttcatcgcg ggagcgatga
      241 cgagcgggcc ggagtgcgcg agtgcagcct ccgtcaggac gctgtcggca agaccgtgcg
      301 cgagcttggc gagcgtgttc gccgtcagcg gcgcgacgac catgaggtcc acaggccgca
      361 agtgcgggta ggtatccgtt tccggcgcgc gccgcgctag agcgtcgaac gtcgctgcgg
      421 cgacgaaccg ctcggcagcg ggagtgagca cgggcaggac ctcgtgcccg gtacggacga
      481 gcagacgcac gagctcgcac gccttgtagg cggcgatgcc cccggagacg ccgacgagaa
      541 tccgcgccat gcacggattc tgccgactcg agtgctaccc gcgcacgtgc ggacgcgtcg
      601 ccgcgaccgc tgtaccccgt ctcaggtcga ggggtactcg tacttgatct tgccctgcga
      661 tacctcttcc atcgacatgg tgaggtagtt cttcgagcgg gactggatca gcggcggcgg
      721 tgcatgctcg aagcccattc cctctccgag ctggtggtga tagctgttga tctgccgggc
      781 ccgcttcgcg gccacgatca cgagcgcgta gcgggagtcg acatgctcca gcagatcgtc
      841 gatgcgaggt tcgatcatgg acggctcatc gtacctgcga gctcgagctc gcgttcgacg
      901 atcgcggtca gcacctcgat cgcgcgcccg acgtcgtcgt ttcgaaccat gtacctgaac
      961 tggtgcgcct gctcgagctg aagccgcgcg agcgcgatgc gctccccgat ctcgcccgtc
     1021 gactcggtcg cgcgctcggt cagtcggcgc tccagctcgg gcacgtcggc ggcgacgaag
     1081 atcgtgaccg ctcccggcac ctcctcctgc acccgcagcg caccctcgag ctcgagctcg
     1141 agcacgcaga tgcggccgtc gttggcgatc cggtcgagtt cggaacggag cgtcccgtag
     1201 cgcttgccgc tgatgtaggc gacatgctcg aggaaggctc ccgactcgat ctggcgcacg
     1261 aactccgcct cgtcgaagaa ccagtagtcg cgtccctcca cctcgcccgg gcgctgcgct
     1321 ctcgttgtcg ccgagacggc gacttcgagc gcgggcacgc gctcgaccag cgccttgatc
     1381 agggtgccct tgccagcgcc ggagggcccg gtgatcacga agacgggctg cgcgttccgg
     1441 gtcagcggtt gaagagggac acgagctccg tgcgttgccg ctcggacatg cccccgacgg
     1501 tcttcgactg gctgatccgg cactgattga gcaggcgcgc ggctttcacg cgcccgaact
     1561 tcggcaccgc catcagcatg tcgaacacct tggccgtcga gacgtagtcg ggcggatcga
     1621 gcaggatcgt ctcgatctgg gctcgtccgt ccttcaaatc cttcttcagc tttgcccgcc
     1681 gaacgcgaat gtcgttggcc cgtttgagcg cctccatcct ctgatcgagc gagcggacgg
     1741 gcgcctgggt ctgcgtcttc gacgtcgcca ttgagcggtc gagtctagca acctcaacgc
     1801 ctcggtcaag cgggaccgtt ttcccctatt tccagggatt tttccacagg gtgcggaggg
     1861 ttttgcacac ccctgtgggc agtcctgtgg gcggattgca gatcgggcag accgtgtgcg
     1921 tcgacggcgg cgagcagctc gctccggatc cgcgacggcg catcggggtc accgaagaga
     1981 actgtcccga gcgcgaccgc cgacgctccg gcggccacga actcgagtac gtccgtgccg
     2041 gtccggatcc cgcccatgcc cacgatcgga aggtcgaccg cgctcgcaca agcgtagacg
     2101 cacgcgagcg cgatcggctt gagggccggg cccgagtagc cgccggtcgc ccgcgcgagg
     2161 accggtcgga gcgtctcggg gtcgagcgcg agaccccgaa tcgtgttgac gagcgacagc
     2221 ccgtccgccc ccgccgcggc aaccgagcgg gcggcttcgg cgacgtccca gctcgccggc
     2281 gagagctttg cgaacaagcg cttgctggtg cttcgccgcg ctgccgcaac cagctcggcg
     2341 gccgtctcag gcgcctcgtc gacgttgggg caggacaggt tgagctcgat cacggcgacg
     2401 cgctcacagt cgtcgaggcg ttcgcactga tctgcgtagt cggctgccga gaagccgccg
     2461 accgacaccc agagcgccgg gccgagctcg acgagagtcg ggagcacatc ggcgacgaag
     2521 gtgtcgattc ccgggccctg cagcccgatc gagttgagca tgccgtattc ggtctcggcg
     2581 atccgcaccg gcggatttcc gtgccgcggc gaaggcgtga tcgtcttcgt cacgaaggcg
     2641 tcgagcgagc gcgcgacctc gggtgcagcg agggcatcga ggcagcccga cgcgttcagc
     2701 agggtgacgc tcatgcgaat cgctccggat ggccccgaag gaccggccct gtcacgcaca
     2761 gacgctcgta gtggccttcc acggtgacga cgcagccgta acaggcgccg tagccacacg
     2821 ccatgggcgc ctcccaggcg agctgtgcgc tcggcaccag ccggcgcaac gccgcgagca
     2881 tcggctcggg gccgcaggca agcacgtcgc ccggatcggg cggcagcgcg tcggtcacga
     2941 gcgtcggctc gagcacgact tccgcgaagg gcaagagctc cgcggcggcc gcctgctcat
     3001 ccgtgcggaa
//
LOCUS       DBNGNK010000110         4580 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_233831,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000110 DBNGNK010000000
VERSION     DBNGNK010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_233831"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            533..1948
                     /locus_tag="AC150V_03135"
     CDS             533..1948
                     /locus_tag="AC150V_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012377.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HIR3761945.1"
                     /translation="MTRNALARLVAVCLAALVAAPAAIAGERMWMGFHDDPVLRYDGN
                     RQVELDRTTQAGATMIRTLVTWADVAPQRPRRAANPFDPAYKLDDLDEFIRNAQARGQ
                     EVLITLWGTPKWANGGKGPAFLPRRMSDFRNFARAIASRYSGRFAGYPYVGFYGVWNE
                     SNLGGFLQPQFDAKGRIVGPKLYAKLAAAGITGLKAGNRKALVAIGETSSNGLDKPKK
                     GVTDKVRPGTFARLVAQANRKLKFDAWAHHPYPVPVSQKATQKVLWPNVSLSSLPRFE
                     KSIDQWFRRKNIPVWITEYGHETKPGEPKGVTEGQQALYMQQAINIVRKDPRVPVFIW
                     FVFRDSKGSLWQSGVYRANGSKKRSYARYRNATPLVDMRNGAYTFKGGTQNPQVTGYV
                     REFCANNAPGTQIGVTYRAFESSELVLVGQSSQPLAVDCTVPVRLQGLTVAKGQRLIV
                     TLELNTALGNGAVRTLQITGT"
     gene            complement(1977..2624)
                     /locus_tag="AC150V_03140"
     CDS             complement(1977..2624)
                     /locus_tag="AC150V_03140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3761946.1"
                     /translation="MDAGRFERELAAIFEDFPASQHPRDRRYERVLEDVGGLARENNL
                     ALIGLAASLLDPGESYVEVGTYLGASLIAALLGNRDADAVAIDTFTFREGSRELLAAN
                     LERFGLGGRADVLEGDVFDLVPAGALGGRTVGVWYYDALHEYEAQLNGLRIVEPHLAP
                     GALLIVDDTDWEQVGRAVHAYLAEQPRARRVLAIDGKDRGQPHWWEGMEVLAWKS"
     gene            complement(2628..3178)
                     /locus_tag="AC150V_03145"
                     /pseudo
     CDS             complement(2628..3178)
                     /locus_tag="AC150V_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549664.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
     gene            3464..4492
                     /locus_tag="AC150V_03150"
     CDS             3464..4492
                     /locus_tag="AC150V_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3761947.1"
                     /translation="MLETPVTVAFVADAEERGKVAVAEIEAFALEHELEEGAPDELRA
                     HLAERGVEVHEEADTADAPVSGPVTHAATADSLQLFLTEVGRHDLLTAADEVTLAKRV
                     ERGDLEAKERMINSNLRLVVSIAKRYRGHGVPFLDLIQDGVIGLNRAVEKFDWRKGYK
                     FSTYATWWIRQACQRSVANQSATIRIPVHVQERQQKLGRTRQRLEATLGREPTLEELA
                     AETGLKVEHVDEALGAAEASVSLNRRIGDDGDGELGDLFADRSSPDPADEAEDSIRYQ
                     DIREALSRLDDPDRRVLELRFGFEGEQWTLEAIGKELGLTRERVRQIETRALTRLQRD
                     LGALAISA"
BASE COUNT          802 a         1556 c         1595 g          627 t
ORIGIN      
        1 cgcgggcggc ggtggcggcg gaggcggtgg cggtggcgag ggcggcgggg cgggcggagg
       61 cggaactgga ggcgacgggg ccggcgacgg cgggggagag ctcggcggag gcgcgacggg
      121 ctccggcatg agcggcggcg gagcaggcgg aggcggtggc ggcggaggcg gtggtggggg
      181 cggcggggcg ggcggcggtt gcggccagca ccactcggga tcgatgcaga cgaacgcggg
      241 ccaggcgcgg gcgccggcat cgccggcaat gccgcccgcg acggccacga cggccgtcgc
      301 gagtgggagg agaacgaggg ccccgagcgc tctcgaggcc ctgcgtcggg cgggcgggga
      361 ctccatgcgt cgtccctcct ttcggtcggg gaaccacgtc cgacgctacg gcggaggttt
      421 caacggatgg gagacgcctt cgcgccgatt tcgcgccggc ggttttcctc acaccgacat
      481 cacatgccgt cggtaccgtg gtgggctgtc ccgccccaag gaggaacgtc ctgtgacacg
      541 caacgccctg gcacggctcg tcgcagtctg tctcgcggcc ctggtcgcgg cgcccgctgc
      601 gatcgccggc gaacggatgt ggatgggttt ccacgacgac cccgtgctgc gctacgacgg
      661 caaccggcag gtcgagctcg accgcacgac tcaagccggc gccacgatga tccgcacgct
      721 cgtgacctgg gccgatgtcg cgccccagcg gccgcgcaga gcggcgaacc ccttcgaccc
      781 ggcctacaag ctggacgacc tcgacgagtt cattcgcaac gcgcaggcgc gcgggcaaga
      841 ggtgttgatc actctctggg ggacgcccaa gtgggcgaac ggcggcaagg gcccagcctt
      901 cctccccagg cgcatgtccg acttcaggaa cttcgctcgg gcgatcgcgt cccgctattc
      961 aggccgcttc gccggatacc cgtatgtcgg gttctacggt gtctggaacg agtcgaatct
     1021 aggcgggttc ctccagccgc agttcgacgc aaagggacgt attgtcgggc ccaagctgta
     1081 cgcgaagctg gctgcggccg ggatcaccgg cctcaaggcc ggcaacagga aggcgctcgt
     1141 cgcgatcggc gaaacatcct cgaacggcct ggacaagccg aagaagggcg tcaccgacaa
     1201 ggtccgtccc ggcacgttcg cgcgcctcgt agcacaggcc aacaggaagc tcaagttcga
     1261 cgcgtgggcc caccatccgt atccggtgcc cgtgagtcag aaggcgacgc agaaggtgct
     1321 gtggccgaac gtctccctct cgtcgctacc gcggttcgag aagtcgatcg accagtggtt
     1381 ccgccggaag aacattcccg tctggatcac ggagtacgga cacgagacga agccggggga
     1441 gccgaagggc gtgacagagg ggcagcaggc tctgtacatg cagcaggcca tcaacatcgt
     1501 gcggaaggac ccgcgcgtcc cggtcttcat ctggttcgtg ttccgcgact cgaagggcag
     1561 cctgtggcag agcggcgtct accgcgccaa cggctcgaag aagcgctcgt acgcgaggta
     1621 tcggaacgcc acgcccctgg tcgacatgcg gaacggcgcc tacaccttca agggcggcac
     1681 ccagaacccg caggtcaccg ggtacgtccg tgagttctgc gccaacaacg cgccgggtac
     1741 gcagatcggc gtcacctatc gggcgttcga gagctccgag ctcgtcctcg tcggtcagag
     1801 ctcgcaaccc ctcgcggtcg actgcacggt gccggtgcgg ctgcagggcc tgacggtggc
     1861 caagggccag cggctgatag tcacactgga gctgaacacc gccctgggca acggagcggt
     1921 tcgcaccctt cagatcacgg ggacctagcg acgaggagca tgaggtgctc gcccggtcaa
     1981 ctcttccagg cgagcacctc catgccctcc caccaatgcg gctgcccgcg atccttgccg
     2041 tcgatcgcga ggacacgtcg tgcgcgtggc tgctcggcga ggtaggcgtg gacggcgcgc
     2101 ccgacctgct cccagtcggt gtcgtcgacg atcaggagag cacccggagc aagatgcggc
     2161 tcgacgatgc ggagaccgtt caactgtgct tcgtactcgt gtagcgcgtc gtagtaccag
     2221 acgcccacgg tgcggccgcc gagcgcccct gccggtacga gatcgaagac gtctccttcg
     2281 agcacgtcgg cccgcccgcc gaggccgaac cgctcgagat tcgccgccag cagctcccgg
     2341 ctcccctctc gaaaggtgaa ggtgtcgatc gccacggcat cggcgtcccg gttaccgagc
     2401 agcgctgcga tcaaactcgc tccgagatat gtcccgactt cgacatagct ctcacccgga
     2461 tcgagcagag acgcggcgag cccgatgaga gcgagattgt tctcccgggc gagtccgccc
     2521 acgtcctcga gcacgcgctc gtagcgccga tcccgaggat gctgcgaagc cgggaagtcc
     2581 tcgaagatcg cggcgagctc gcgctcgaaa cggccggcgt ccacggttca ggcagctgcg
     2641 gcgtaggcgc cgagctggtc gaggagctgc actgggtcgt ccgacacgat caagctctcc
     2701 cgggtagagc gccccacgaa gccttccgcc gctgcgtggt cgacgaacgc gagcaggtgc
     2761 cggaagtagc cctcgacgtc gaggagacca atcggcttgc gatgcagacc gagctgccgc
     2821 cacgtgacgg cttcggccag ctcatcgagc atgccgagac caccgggaag cgcgacgaag
     2881 gcgtctgcga gatccgccat cagcgccttg cgctcgtgca gagtctccac gacgtggagc
     2941 tcctggaggc tctcgtgagc gagctcacga tcacggagcg cgcgcggcaa gacgccgacg
     3001 acgccgcccc cttctctccg agccgcgtcc gccacgacgc catcagcccg acccggccgc
     3061 cgccgaagac cagcccgaga ccacgcgcgg cgagcaggct gccgaatgcc gcggcggcct
     3121 gcgcgtgctc ggggcggaca cccttcgacg agccgcagaa gacacagacg ctgaacacgc
     3181 cccgcagcct atcggcggcc gctatgatcc ccctccgccg gaacccccgc atgggggaat
     3241 agacgtccgt gcgttttcgt tatactcctt cgacggcgca gtgccgcgag ctttcgtgtg
     3301 ctctgaatgc attccgcagc gaggcggcac cgcgtcaacc gccccccgag tcgccgcacg
     3361 gcgtgtgcag ggagcacgcg gccgggaggc agcgcgggga gcttgtcgaa cagaaccgag
     3421 agaaagaccg aaagccgaag agaaaggaag aacgctgagc accatgctgg aaacccccgt
     3481 caccgtggcc ttcgtcgccg acgccgagga gcgtgggaag gtcgccgttg ccgagatcga
     3541 ggcgttcgcg ctcgagcacg agctcgagga gggagcgccg gacgaactgc gagcgcacct
     3601 ggccgaacgc ggcgtcgagg tgcacgagga ggccgacacc gccgatgcgc cggtgagtgg
     3661 ccccgtgacg cacgcggcca cggcggactc actgcagctg ttcctcaccg aggtcggtcg
     3721 ccacgacctg ctgactgccg cagacgaggt caccctggcc aagcgcgtcg agcgaggtga
     3781 tctcgaggcg aaggagcgca tgatcaactc caacctgcga ctcgtcgtct cgatcgccaa
     3841 gcgctaccgc ggccacggag tgccgttcct cgatctcatc caggacggcg tcatcggcct
     3901 gaaccgcgcg gtcgagaagt tcgactggcg caagggctac aagttctcga cctacgcgac
     3961 atggtggatc cgacaggcgt gccagcgctc ggtcgcaaac cagtcggcca cgattcgcat
     4021 cccggtgcac gtccaggagc gacagcagaa gctgggccgg acgcgccagc gcctggaggc
     4081 gacactcggg cgagagccga cactcgaaga gctggcagcc gagaccgggc tgaaggtcga
     4141 gcacgtcgac gaggcgctcg gcgccgcgga ggcgtccgta tcgctcaacc gccgcatcgg
     4201 cgacgacggc gacggcgagc tcggcgatct gttcgccgac cgcagctcgc ccgaccccgc
     4261 cgacgaggcc gaggactcga tccgctatca ggacatccgc gaagctctct cgcgtctaga
     4321 cgatcccgac cgccgcgtgc tcgagctccg tttcggcttc gagggcgagc agtggacact
     4381 cgaggcgatc ggcaaggagc tcggcctcac ccgagagcgc gtccgccaga tcgagacgcg
     4441 ggcgctcacc cggctccagc gcgacctcgg cgcgctcgcg atctccgcgt agagcagaag
     4501 cacgaggggg acggtcccgg cgacggggcc gtcccccgtt tctacgagtt gaacagggtc
     4561 agctcgggct cgcgcgcgcc
//
LOCUS       DBNGNK010000111         4133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_734727,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000111 DBNGNK010000000
VERSION     DBNGNK010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_734727"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="AC150V_03155"
     CDS             <1..1815
                     /locus_tag="AC150V_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3761948.1"
                     /translation="FVEELVGALVDAGVLEQADDEWVAHDLPSGFSVPDSVHAVLAAR
                     IDHLPQAEKAALQAASVIGRVFWPAPLTHLLDGREPDLVLLEERDFIRRRGRPSTDGV
                     DEFAIKHALTREVSYAGIPKARRGRLHAAFADWLRSTDPGKEEHASLLAHHYAEAVRP
                     EDADLVWADAPEELARLRAGAVEWLRRASALAGHRYEIDEGVELLERAVPLASGPEER
                     SDLWREVGMLNALRFDGDGFVAAMERALAEAPGRDSAARTAGVLALYASLQSGMWQRR
                     PQADVLESWITQALELAEPDSIDRARALVARVHLDASAATTEAAEALEIAERLADADL
                     LSFALEARANVAFERGLYAESMDWMDKRLPLLPKLRADASTELLDGVVPAANAVGRFD
                     QARKLAEQHAERSRSLSAHHRLHGVSLCVEVEEHVGDWSAVNELRDRACQAIEENLAT
                     PCVRHSRTLMVCAIAAACAGDDVATSELLREEASLEMAGYDYAVDAPRLRLALLRGDG
                     VTARRLVERPPARTFVFGAVAHAVRLDALVAVRDRAGAERVAAALLRPGAYVEPFALR
                     ALGWARRDDALLEQSDERFRALGLDWYADQTEALIAGV"
     gene            complement(1826..2827)
                     /locus_tag="AC150V_03160"
     CDS             complement(1826..2827)
                     /locus_tag="AC150V_03160"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017549509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR3761949.1"
                     /translation="MRIAVLAGGRSSEHDVSVASARSVLEALDSERYEATAIAIGRDG
                     RWQLPGATNGALPATTAETLPVPADSAPATLGKVDVVLPILHGPFGEDGTVQGLLELA
                     GVPYVGAGVAASAVCMDKDLFKAVLRDRGIPVARHLVIREGDPVGNPFGYPVFVKPAR
                     LGSSVGISKVSTEGELEPAVELARRHDDKVLVEEFVPGMEVECGVLGNREPIASEVGE
                     IVAHADWYDYAAKYDEGGMDLIVPARLSPDATRRVQKLSVESFVATECEGMARVDFFV
                     RPDGEVVVNELNTIPGFTATSVYARLFEAAGVPYSELLDRLIELALERHERRARLEY"
     gene            complement(2836..>4133)
                     /locus_tag="AC150V_03165"
     CDS             complement(2836..>4133)
                     /locus_tag="AC150V_03165"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR3761950.1"
                     /translation="LRALAKEPADRYRSAAEMDRDLELVGRGEPVGSETADAATMVLA
                     GAGAVEAATAATEVRRSPAYAGDERYRAYDATPTRRRSVWPWLLAAAAVIGLAAAGYF
                     LYDDVRQQLEDSKPVAVRDYEGLRELNAVALIKEDGFEARVAREPSQDTPPGIVFDQT
                     PPAGTQLQKESVVQILVSSGKPTVTIPDVRGQSLADGVAALSRLGLEANVVEVNSGEP
                     AGTVTGQAPVAGEVVVSGATVRINVSVGPKQVAVPSVTGLFYETAAAQLQAGGFAVAR
                     RDVDSDLPKDTVATQDPAGNTTATKGSTVTLSVSRGPQTIPVPDVTGLDIATARATLN
                     ASGFKVNVITQDTDDLSLDAVVISQEPLANAEQEPNTTVTVNLGRYVEPPPNVIPDDL
                     PGGTIPPDEPAPAPVTPPPATPPPAQPPPAQPPPAAVTP"
BASE COUNT          576 a         1429 c         1484 g          644 t
ORIGIN      
        1 ttcgtcgagg agctcgtcgg cgcgctcgtc gacgcgggcg tgctcgagca ggccgacgac
       61 gagtgggtgg cccacgacct gccgagcggt ttctcggtcc cggactccgt tcacgctgtt
      121 ctcgccgccc ggatcgacca tctgccgcag gcggagaagg ccgctctgca ggcggcctcg
      181 gtgatcgggc gagtcttctg gccggcaccg ctgacgcacc ttctcgacgg ccgcgagccg
      241 gatcttgtcc tgctcgagga gcgcgatttc atccgcaggc gagggcggcc gagcactgac
      301 ggcgtcgacg agttcgcgat caagcacgcg ctgacgcggg aagtctccta cgccgggatc
      361 ccgaaggcgc gccgggggag gctccacgcg gcgttcgccg actggctccg gtcgacagac
      421 ccgggcaagg aggagcacgc ctccctcttg gcgcaccact acgccgaagc cgtccgcccg
      481 gaggacgcgg atctcgtctg ggcagacgct ccggaggagc tcgcgcggtt gcgggcgggc
      541 gcggtggaat ggttgcgccg cgcgtccgcg ttggccgggc accggtacga gatcgatgag
      601 ggtgtcgagc tgctcgagcg ggcggttccg ctcgcctcgg gccccgagga gcggtcagat
      661 ctctggcggg aggtcggcat gctcaacgcc ctgcgcttcg acggtgacgg gttcgtcgcg
      721 gcgatggaga gggccctggc cgaggccccc ggccgtgact cggcggcgcg aaccgccgga
      781 gtgctcgccc tctacgcctc actccagtcg ggtatgtggc agcggcggcc gcaagcggat
      841 gtgctcgagt cgtggatcac ccaggcgctc gagctggccg agccggacag catcgaccgg
      901 gcgcgtgccc tggtcgcccg agtgcatctc gacgcttcgg ctgcaacgac ggaggcggcc
      961 gaggcgctcg agatcgcgga acggctcgcc gacgccgatc ttctctcctt cgcactcgag
     1021 gcgcgggcga acgtcgcctt cgaacgggga ctctacgccg agtcgatgga ctggatggac
     1081 aagcgtctgc cgctgctgcc gaagctccgg gccgatgcct cgaccgagct gctcgacggg
     1141 gtggtcccgg cggccaacgc ggtgggccgc ttcgaccagg cacgcaagct cgcggagcag
     1201 cacgccgagc gatcgcgttc cctctccgcg caccatcgtc tgcacggcgt ctccctctgt
     1261 gtcgaggtcg aggagcacgt cggggactgg agcgctgtca acgagcttcg cgatcgcgct
     1321 tgtcaggcga tcgaggagaa cctcgcaacg ccgtgtgtcc gtcactctcg cacgctgatg
     1381 gtgtgtgcga tcgccgctgc atgcgcgggc gacgacgtgg cgacgtccga actgctccgg
     1441 gaggaggcgt cgttggagat ggcggggtac gactacgcag tcgacgcgcc gcgactgagg
     1501 ctggccctcc tgcggggaga cggcgtgacc gcacgtcgcc tcgttgagcg gccacccgct
     1561 cgcacgttcg tgttcggggc ggtcgcgcac gccgtacggc tcgacgcgct cgttgccgta
     1621 cgcgatcgcg cgggagccga gagggtggcg gcggcgcttc tacgtccagg agcctacgtg
     1681 gagcccttcg cactgcgagc cctcggttgg gctcgccggg acgacgcact gctggagcag
     1741 tccgacgaac ggttccgtgc gctcggcctc gactggtatg cggaccagac ggaggcgttg
     1801 atcgcgggcg tctagtccgg ccggatcagt actcgagccg cgcgcggcgc tcgtgccgct
     1861 cgagagccag ctcgatcagg cggtcgagca gctcggagta cgggacaccc gcggcttcga
     1921 acagccgtgc gtagacgctc gttgcggtga aacccgggat cgtgttgagc tcgttcacga
     1981 cgacctcgcc gtccggccga acgaagaagt cgacacgggc cattccttcg cactccgtgg
     2041 cgacgaagct ctcgacggag agcttttgta cccggcgagt cgcgtccggc gagagccgcg
     2101 ccggaacgat caagtccatc ccgccctcgt cgtacttggc tgcgtagtcg taccagtcgg
     2161 cgtgcgcgac gatctccccg acttcggacg caatcggctc gcggtttccg agcacgccgc
     2221 actcgacctc catccccggg acgaactcct ccacgagaac cttgtcgtcg tggcgacggg
     2281 caagctccac cgcgggctcg agctcgcctt cggtgctcac tttggagatc ccgaccgacg
     2341 acccaagtcg cgcgggcttg acgaacaccg ggtagccgaa agggttcccg accggatcgc
     2401 cttcccggat gacgaggtgt ctcgcgaccg ggatgccccg atcccgcagg acggccttga
     2461 agagatcctt gtccatgcag accgccgatg cggcgacacc agccccgaca tagggaactc
     2521 cggccagctc cagcagcccc tgcaccgtgc cgtcctcgcc gaacggtccg tgcaagatcg
     2581 gcaggacgac gtcgaccttg ccgagcgtgg cgggcgccga gtcggcgggc acgggcagcg
     2641 tctccgcggt ggtggcgggg agcgcgccgt tcgtggctcc cggaagctgc cagcggccgt
     2701 ctcgaccgat cgcgattgcc gtggcctcgt atcgctcgga atcgagcgcc tcgagcaccg
     2761 atcgtgccga ggcgaccgag acatcgtgct cgctggatcg cccccctgcg aggaccgcga
     2821 tccgcaacct gggactcagg gggtcaccgc ggcgggaggt ggctgggcgg gaggtggctg
     2881 ggcgggcggc ggtgtggcag gtggtggggt gactggcgcg ggcgccggct cgtcgggtgg
     2941 gattgttcct cccggcaggt cgtcagggat gacgttcggc ggtggctcca cgtagcggcc
     3001 gaggttgacg gtgacggtcg tgttcggctc ctgctctgcg ttcgcgagcg gctcctgcga
     3061 gatgacgacg gcatcgagcg acagatcgtc tgtgtcctgc gtgatgacgt tcaccttgaa
     3121 gccggaggcg ttgagcgtcg cccgggcggt ggcgatgtcg aggccggtca cgtccggaac
     3181 cggaatcgtc tgcgggccgc gcgacaccga cagcgtgacg gtcgagccct tcgtcgccgt
     3241 cgtgttcccg gccggatcct gggtcgcgac cgtgtccttc ggcaggtcgg agtcgacgtc
     3301 ccgacgcgcc acggcgaagc ctcccgcctg cagctgggcg gcggccgtct cgtagaagag
     3361 gcccgtgacg gaagggacag cgacctgctt cggtccgacg gagacgttga tgcggacagt
     3421 cgcccccgac acgacgacct cgccggccac cggcgcctga cccgtcacgg tccccgccgg
     3481 ctcacccgag ttcacctcta cgacgttggc ctcgaggccc agacgggaca gcgccgcgac
     3541 gccgtccgcg aggctctggc cgcggacgtc ggggatggtc accgtcggct tgccggacga
     3601 gaccaggatc tgtaccacgc tctccttctg gagctgggtg ccggccggcg gtgtctggtc
     3661 gaacacgatc cccggcggag tgtcctggct cggctcacgg gccaccctcg cttcgaagcc
     3721 gtcctccttt atgagcgcca ccgcgttcag ctctcgcaga ccctcgtagt cgcgaactgc
     3781 gacgggcttg gagtcctcga gctgctgccg gacgtcgtcg tacaggaagt agccggccgc
     3841 cgccaggccg atgaccgcgg ccgcagcgag gagccacggc cagaccgacc ggcgcctcgt
     3901 cggggtggcg tcgtacgccc gatagcgctc gtcccccgcg tacgcgggcg agcgccgcac
     3961 ctccgtcgcc gccgtcgccg cctcgacggc gcctgcaccg gccagaacca tcgtcgccgc
     4021 gtcggcggtc tccgacccga ccggctcgcc gcggccgacc agctcgaggt cgcggtccat
     4081 ctccgcggcc gaccgatagc ggtcggccgg ctccttcgcg agcgctcgca gga
//
LOCUS       DBNGNK010000112         3442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_91179,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000112 DBNGNK010000000
VERSION     DBNGNK010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_91179"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="AC150V_03170"
     CDS             <1..108
                     /locus_tag="AC150V_03170"
                     /inference="COORDINATES: protein motif:HMM:NF036805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761951.1"
                     /translation="LPPVYADVPPVAHPLAIRQILAHLERLESLGEIRR"
     gene            complement(264..1076)
                     /locus_tag="AC150V_03175"
     CDS             complement(264..1076)
                     /locus_tag="AC150V_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3761952.1"
                     /translation="MTISTCLIAVALLAPGTGRADDCKYTRPLDLDLDAAGATLLEVD
                     AEAGFLVIEGERGRTRVVVEGVACASSEGMLKDIALDGGRRGDRLHVEAIIPDFDFSW
                     RGYARLDLTVRVPDDLALRVRDGSGEIEIYRVASVELRDGSGAIDISEVYGDVGITDG
                     SGEIEVRDVDGLVRVSDGSGGVVISGAGGGVHVEEDGSGELRITAVRGDVEIEEDGSG
                     GITIRDVTGTVRVGSDGSGSIWVAQVSGGFELGSDGSGSVHVEDVSGTVKMP"
     gene            1280..3331
                     /locus_tag="AC150V_03180"
     CDS             1280..3331
                     /locus_tag="AC150V_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR3761953.1"
                     /translation="MRNSEPSSSPPRAPRRPVARTDHGRERVDPYAWLADRGDEATTA
                     YLEAENAYSRAALAHLEGLEKALYEEFLSRIKETDLTVPIRLDDYRYYRRTEKGRQYP
                     VYCRKRGSLEADEEILLDVNALAAGGDYLRLGAMEVSPDHGLLAFCVDLSGGEAFELR
                     IKSLATGELLPDRIDGVAGSIGWANDNRTLLYTVLDAAKRPYKVLRHRLGDESGDRMV
                     YREDDEAFFVSVERTRSRDYLLITSASAVTSAVRYLSTGRPAEAPAVVEPRRQGVEYY
                     VEHHGDSFYLLTNGGAVNFRLVRRPIGGGARDDVEILSHREDVKLERVDPFEDHLVVW
                     ERRDGLLELRIRSLTTGEDRYLDLPEPVYTLRREPNPEFRAGELRFGYTSMVTPSTVF
                     SCDMESLELEVLKVQEVPGGYDASRYVTGRLRVDSGGVSVPVSYVHRRDLAIDGSHPC
                     LLHGYGAYGAVVDPVFSPILISLLDRGFVYAIAHVRGGGLLGKPWHENGRMLAKRNSF
                     EDFVAVAEHLIATGYSHPELLAIRGGSAGGLLVGAALNMRPGLFRAAIARVPFVDVVN
                     TMLDPSIPLTVIEYEEWGNPQEERFFEYMLSYSPYDNVAAQEYPALLVTAGLNDPRVQ
                     YWEPAKWVARLRELKTDANLLVLRTHMGAGHAGPSGRYEALSERAQEYAFLLGVMGVA
                     E"
BASE COUNT          511 a         1258 c         1135 g          538 t
ORIGIN      
        1 ctcccccccg tctacgcgga cgtcccgccc gttgcccacc ccctggcgat ccgccagatc
       61 ctcgcccacc tcgagcggct cgagtccctg ggcgagatcc ggaggtgaag ggccggagag
      121 ggtacgaggg tcctcctccg cgaccggctc gcttctgggg ggcggggggt atggctcgcc
      181 ttatcgttcc gctcgccctt ccccgcacac ccccggcccc ggcagccccg ggtcggcttt
      241 ccgcggcgct tgcgcttgcc cggctagggc atcttcaccg tgccgctcac gtcctcgacg
      301 tggaccgagc cgctgccgtc gctgccgagc tcgaagcctc cgctcacctg tgccacccag
      361 atcgatccgg agccgtccga gccgacacga accgtgccgg tgacgtcgcg gatggtgatg
      421 ccgcccgagc cgtcctcctc gatctcgacg tcgcctcgca ctgcggtgat gcggagctcg
      481 cccgagccgt cctcctcgac gtgcacgcca ccgccggcgc cgctgatcac gacgcctccg
      541 gaaccgtcgg acaccctcac cagaccgtcg acgtcgcgca cctcgatctc gcccgagccg
      601 tccgtgatcc cgacgtcacc gtagacctcc gagatatcga tcgctcccga gccgtcccgc
      661 agctcgacgc tggcgacccg gtaaatctcg atctcgcccg atccgtcgcg gacgcgcagc
      721 gcgaggtcgt cgggaacgcg gaccgtcagg tcgagtcgcg cgtagccgcg ccacgagaag
      781 tcgaagtccg gaatgatcgc ttcgacgtgg agacggtcgc cccggcggcc gccgtcgagc
      841 gcgatgtcct tcagcatgcc ctcgctcgag gcgcaggcga cgccctcgac gacgacccgg
      901 gtacggccac gctccccttc gatgaccagg aagccggcct cggcgtcgac ctcgaggagc
      961 gtcgcgcccg cggcgtcgag gtcgaggtcg aggggccggg tgtacttgca gtcgtcggcg
     1021 cgcccggtgc ccggcgccag cagcgcgacc gcgatgagac aggtggagat cgtcaatgga
     1081 cgcatcgaat cctcccagtt tctttgcagt tcttgtcaga ccttacgaca gttggacacc
     1141 tgggtttcac gaaaggttga caccgagcct cggctgccga gctcggaggc aagagtcgaa
     1201 tggagtccac agatcgccgt gctagcctcg ccggcgggga ggtcgccaga gcggccggcg
     1261 ggccgcaccc ccggccctga tgcgcaattc cgagccatcg agttcgccgc cgcgcgcgcc
     1321 gcggcgtccg gtcgcgcgaa ccgaccatgg tcgggagcgg gtcgatccat acgcctggct
     1381 cgccgaccgg ggcgacgagg ccacgacggc ctacctcgag gcggagaacg cctactcgcg
     1441 ggcggcgctg gcccacctgg agggcctcga gaaagcgctc tacgaagagt tcctgagccg
     1501 catcaaggag accgacctga cggtcccgat ccggctcgac gactatcgct actaccgccg
     1561 caccgagaag ggccggcagt atccggtcta ctgcagaaag cgaggcagcc tcgaggccga
     1621 cgaggagatc ctgctggacg tcaacgcgct cgccgccggc ggagactatc tgcgcctcgg
     1681 cgcgatggag gtgagccccg accacgggct gctggccttc tgcgtcgacc tctccggcgg
     1741 cgaggcgttc gagctgcgga tcaagagcct cgcgaccggc gagctgttgc cggaccggat
     1801 cgacggcgtg gcgggatcga tcggctgggc gaacgacaac cggacgctgc tctacacggt
     1861 tctcgatgcc gccaagcgac cctacaaggt cctccgccac cggctgggcg acgagagcgg
     1921 agaccggatg gtctaccgag aggacgacga agccttcttc gtctcggtcg agcgcacccg
     1981 cagccgcgac tatctgctca tcacctcggc gagcgcggtg accagcgcgg tgcgctacct
     2041 gtcgaccggg cggcctgccg aggcccccgc cgtggtcgag ccgcggcggc agggcgtcga
     2101 gtactacgtc gagcaccacg gagattcctt ctatctgctg accaacggcg gcgcggtcaa
     2161 cttccggctc gtgcgccggc cgatcggtgg gggcgccagg gacgacgtcg agatcctgtc
     2221 ccaccgggag gacgtcaagc tcgagagggt cgaccccttc gaggaccacc tggtggtctg
     2281 ggagcgccgc gacggccttc tggagctccg gatccgctcg ctgacgacgg gcgaggaccg
     2341 ctacctcgat ctgcccgagc cggtctatac gctgcgccgc gagccgaacc ccgagttccg
     2401 cgccggcgag cttcgcttcg gctacacctc gatggtcacc ccgtcgaccg tcttctcgtg
     2461 cgacatggag agcctcgagc tcgaggttct caaggttcag gaggtgcctg gcggctacga
     2521 cgcctcgcgt tacgtcaccg gccggctgcg ggtcgactcc ggtggtgtct cggtgccggt
     2581 ctcctacgtg catcgccgcg atctggcgat cgacggcagc catccgtgcc tgctccacgg
     2641 ctacggcgcc tatggcgccg tggtcgatcc ggtcttctcg ccgatcctga tctcgctgct
     2701 cgaccggggc ttcgtctacg ccatcgcgca cgtccgcggc ggcggcctcc tcggcaagcc
     2761 ctggcacgag aacggccgga tgctcgccaa gcggaactcc ttcgaggatt tcgtagccgt
     2821 ggccgagcac ctgatcgcga cgggctactc ccatccggag ctgctggcca tccgcggcgg
     2881 cagcgccggc gggctgctcg tcggcgccgc tctgaacatg cgtcccgggc tgttccgcgc
     2941 cgccatcgca cgggtgccct tcgtcgacgt cgtcaacacc atgctcgacc cctcgatccc
     3001 gctgaccgtc atcgagtacg aggagtgggg caacccgcag gaggagcgct tcttcgagta
     3061 catgctctcc tactcgccct acgacaacgt cgcggcccag gagtacccgg cgctgctggt
     3121 gacggcgggc ctcaacgacc cgcgcgtcca gtattgggag ccggccaagt gggtcgccag
     3181 gctccgcgag ctcaagaccg acgccaacct gctggtgctg cgcacccaca tgggcgccgg
     3241 ccacgccggc ccctccggcc gctacgaggc gttgagtgag cgggcgcagg agtatgcgtt
     3301 cctgttgggg gtgatggggg tggcggagta gtagcgagta gcgccactac tgccccgccc
     3361 ccgcgaaccc cccggcctct tcttccgtta tcccaattga cgccccatcc acgcccagcc
     3421 ggaggcttcc atgtcccaac gc
//
LOCUS       DBNGNK010000113        11797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1451699,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000113 DBNGNK010000000
VERSION     DBNGNK010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1451699"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="AC150V_03185"
     CDS             complement(<1..922)
                     /locus_tag="AC150V_03185"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3761954.1"
                     /translation="MARGSRQRHSKLGGAVRLGGALLALVIAVLAVLSLVPSSTDGFW
                     SQPNPASGYEEAVSRFKKIRAGERKRVYEPCKSRLLDHGEQTDVAVVLVHGLTNCPRQ
                     FLDLGDRIHATGANVLIPRAPHHGLADGSGKAIGGVGNVGDLSPEDLRDYADDVVDLA
                     AGLGKDVRVLGFSMGGSVATWIAQNREDVGRVVAVAPALTLPKMPGVVDFLFVNLFNR
                     LPNISLPGRSTPSHSYDGESTRALAAVYKFGKAVREAADSVPARAGSVVVVTNANDGE
                     VDNGDIRALGETWSEMGADVRTHEFPKRLGL"
     gene            956..1561
                     /locus_tag="AC150V_03190"
     CDS             956..1561
                     /locus_tag="AC150V_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3761955.1"
                     /translation="MRRAFAAVFVAVLAIVGAADAPAAKKLRVTVVADSVGASLNYVD
                     AARKLLARGRVLTLDLKVCRRLVQPSCTYQGARPTNALPAVRAHGRRLGDVLVIKVGY
                     NESAKGYRAGIDRVMRAALANGVEAVVWVTLREQRDTYRWTNIAIRSAAKRWKRLTVS
                     DWARYSSGRPWFASDGLHMTPLGARALARFLNASIRAAAPG"
     gene            1577..2503
                     /locus_tag="AC150V_03195"
     CDS             1577..2503
                     /locus_tag="AC150V_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005315118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761956.1"
                     /translation="MESREHLARRDRVVSARGVTKRYGSTVALDGLDLEIGPGITGLL
                     GSNGAGKTTFISLALGIRTRDAGELVVLGEDPAQAGIHVRARIGYAPEHHDLPPDVQA
                     ADLVRHLAELHGLPGQAAIQRANDALWQVGLGEERFRPIGTMSTGQRQRVKLAAAIAH
                     DPELVLLDEPTDGLDPVQRDDMLALIRRVGTEFGMDMLISSHHLEEVERICDGAVIID
                     AGTVIRSGSLAELRRGEDGLVVEVDDRAAELCALLRDAGLEASAEGTLVLVSGTDGVH
                     DTIRDAVAELGVGLRRLEPRSRTLEDVYLEAT"
     gene            2500..3381
                     /locus_tag="AC150V_03200"
     CDS             2500..3381
                     /locus_tag="AC150V_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3761957.1"
                     /translation="MSTDRARIYDVGYRSYGGPRRATAWAMVTLGRHTIQRVLGLRRG
                     FRHKILPLLALLVSFVPAIAYVSLAVLLPQSLLEEDILISYGEYYFFIGTALALFAAF
                     VAPEALCTDRRTGMLDLYLAGPLDLKRYLGAKWLAVYVAMSLMTVGPLLFMLIAYSIE
                     DAGPTWGELPLLLLRIVAAGAVVALLYAAVSLGVSSLTARKGVAAISVVLLLFVPSIV
                     VGVATESADAPDELAIAVAPSVAAELPVRIFGEESEGDGAAQQLATGLIVAGWLVWVA
                     AGALVCLFAYRRLERRR"
     gene            3378..4298
                     /locus_tag="AC150V_03205"
     CDS             3378..4298
                     /locus_tag="AC150V_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3761958.1"
                     /translation="MSGPLVKADGVSKWFGGVVAVSDVSFDIGPGVTALLGPNGAGKS
                     TMLRMLCGLARPSKGSVSVLGRDPRSDVAVTRAIGLAPQQESVFEPLTAAEFVRLIAV
                     LHGVDDPAGAAARALETVELDPDDTRRLPTYSKGMRQRVKLAQAIVHDPQVIVLDEPL
                     AGLDPRQRVHMIRLFHRLGGEGRCVIVSSHVLEEVERFGSDVLVLSRGRLAASGDYRE
                     IRALMDDRPRRVRVRTDRPHELAGALLTRGVVVGARVQDDGALVLDAEDAGRLAHLLA
                     PLAREHAASLLEVRSVDEDLEGVFRYLVER"
     gene            4295..5044
                     /locus_tag="AC150V_03210"
     CDS             4295..5044
                     /locus_tag="AC150V_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761959.1"
                     /translation="MTATPTTVPGGRLGHSSPFRVLFRLLLRMQLTPVRVVAISALGL
                     LSIGLAGLARADDDPLRATVEGAAAYGLGLVLPLSVLWLATSSIGDLVEDKLLVYVWL
                     KPVPRWQLPGAAILATVAIVLPLVVAPLVVAVVVAGQELLVGATLLASALAALAYGGL
                     FVAAGMWLRRALWWGLLYILVWENGIARAADGAARLSVASYTQSILADAGDVDLALGG
                     RSLVASIVVPLTVAGAGCALAVWRYRTADVD"
     gene            complement(5041..6246)
                     /locus_tag="AC150V_03215"
     CDS             complement(5041..6246)
                     /locus_tag="AC150V_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3761960.1"
                     /translation="MPTGPEPDLGSFVLILAAAVAAPLLARASRRIVVPTVVVEVFLG
                     ILIGPQVLDLAQVDEFIVFFSDIGLAFLFFLAGLEIEAAQVRGRLGVLATAGWFSSAA
                     IAVAAALLLFAADVLSGPVVVGIALATTTLGVLTPVLRDNGLSGTRLGTSATGAGVAG
                     ELFPIIFIAVFLSADYSAAHEFVLLVAFFLVCVGAAIVALRARPPHVLRTIAETVHRS
                     GQLAVRLSVFTLILMVWIAVRLDVDFILGALAAGLVVGLVARGETGTIVRDRIEAVGY
                     GLFVPMFFVVSGMRFDLDALLESTTALVLLPVATLVLLAARGLPALILYRRDLDRAGR
                     FSLAFFSATSLPLIVAITEIGVREGDLSRDVAAALVGAGMISVLVFPSVALILRKGSV
                     HGTPVAEAI"
     gene            complement(6262..6822)
                     /locus_tag="AC150V_03220"
     CDS             complement(6262..6822)
                     /locus_tag="AC150V_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761961.1"
                     /translation="MADEPQRTEQEDAANGVADELHRLNEQLAALQREVGRLGGGPLP
                     SVAGDDRPDEAPSYAWVGSLEPRLRRRPALPRFLLEAAFLVAVAALAAAADLEPYVIA
                     LAMAGAWVIVALAEWAASRAERRREEMLLAPPPVPAAPAAADPAWFSPPVEHTQIESA
                     AESETAVTRLPPVADEATVEHKPASS"
     gene            complement(6824..7087)
                     /locus_tag="AC150V_03225"
     CDS             complement(6824..7087)
                     /locus_tag="AC150V_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761962.1"
                     /translation="MRRTLFLAGGIALAAALARAARRRRRPGAELEPVPDPAEELRRT
                     LDSTREVGGVEPEAKRPSLEERRARVHSKAQEALDLMHEQGDR"
     gene            7240..7608
                     /gene="rpsF"
                     /locus_tag="AC150V_03230"
     CDS             7240..7608
                     /gene="rpsF"
                     /locus_tag="AC150V_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR3761963.1"
                     /translation="MTEYEILLLLEPELSEEAQADVVARTRALLEKSGGSLDRHDVWG
                     QRKLAYEIDHKDEGWYHLLLLTCSAETLEEVGRVLRIDDGVMRHMATRRYETSPTEPL
                     AVGASASQDAGETQPAAEED"
     gene            7612..8046
                     /locus_tag="AC150V_03235"
     CDS             7612..8046
                     /locus_tag="AC150V_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR3761964.1"
                     /translation="MAAINSVTLVGNLTRDPELRHTPSGTPVCSLRLAVNTRRKDGAS
                     GQWVEKPNYFDITVWGQQGENCAQYLAKGRQVGVQGRLEWREWETQEGAKRQAVEVVA
                     DTVQFLGSRGEAEGGGQYVPAAAAAQTPDADFSSAGDDDIPF"
     gene            8064..8318
                     /gene="rpsR"
                     /locus_tag="AC150V_03240"
     CDS             8064..8318
                     /gene="rpsR"
                     /locus_tag="AC150V_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR3761965.1"
                     /translation="MAQKTQRRRQSPSTAPGRRKSCHFCRDKVADVDYKNANQLRRYV
                     SEKGKIRNRRITGACRRHQKQVAVAVKRAREMALLPYVAS"
     gene            8328..9095
                     /gene="rplI"
                     /locus_tag="AC150V_03245"
     CDS             8328..9095
                     /gene="rplI"
                     /locus_tag="AC150V_03245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR3761966.1"
                     /translation="MILLTDVEKVGLRGEVVNVARGYARNFLLPRGLAETASPGRVAE
                     LRRIEAERARHEARSADQADEIAEILGKTVLRFDVKAGPTGVLFGSVTPTDIADEIWR
                     ARKVRVDRRKIGVDSIKRIGRYAVEIDVYEGVIAEVKTLVVPEGGELPPEEELAAMAE
                     EQAAEEAAAAEAAVEEHEQAEVAVAEAIEVLEAEDAEEAEEADEAEEGVEAAEADAGG
                     EPEHTAAAPVATAESPEEPEAAAGEEPVDEPTAPPAA"
     gene            9469..10815
                     /gene="dnaB"
                     /locus_tag="AC150V_03250"
     CDS             9469..10815
                     /gene="dnaB"
                     /locus_tag="AC150V_03250"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR3761967.1"
                     /translation="MTELAEPRTSAHVPPQNLEAEESVLGAMLLSPTAIGVVTEILSA
                     EDFYRESHGTIYRAALSLWGKGEPVDAITLADELDGRSELESVGGAARIAELAALVPA
                     ASNVEHYARIVREMATLRGLVRVGTEIARLGRDRPGETPDLVDRSEQLVFELAQERVT
                     TDFARIDVLLREGFERITHLYEQGAELTGVPSGFRDLDSLTSGFQPGNLIILAARPSM
                     GKSALALCAAANLGVREEIPVALFTLEMSKAEVTQRMMCSEAKVESQRLRSGKLHPDD
                     WPRLTAACDRLMKAPIFVDDSGSITMMELRSKARRLKSRESDLGLIIVDYLQLMTSGA
                     SVENRVQEVSQISRSLKVLARDLDVPILALSQLSRAVEQRHDKRPILSDLRESGSNEQ
                     DADLVFFVYRDEYYAGEESDQQGLAEVILAKHRNGPTDAVKLSFLKRYAKFADLAA"
     gene            10812..11228
                     /locus_tag="AC150V_03255"
     CDS             10812..11228
                     /locus_tag="AC150V_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015754413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3761968.1"
                     /translation="MTRDAMAEFPTPEVALTDILVVADLGGSLDWYTHVLGARLEREY
                     GGTSAVLEFQGVWLLLVTEGGPTPDKPSVLFAPPADPDLVSHSFTIRVPDCEAAYRTL
                     KARGAAFLTPPLDRGSEVRCFFRDPDGHLFELSESR"
     gene            11289..>11797
                     /locus_tag="AC150V_03260"
     CDS             11289..>11797
                     /locus_tag="AC150V_03260"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR3761969.1"
                     /translation="MPATVIVGAQWGDEGKGKIVDLLASESDLVCRYQGGPNAGHTIV
                     AEGETYKIRQIPSGIIRGKASAIGAGCVVDPTVVIGELDELESRGISTAGLVFVSGNA
                     HLIMPWHVALDGARERRLGRLQIGTTRRGIGPAYADKATRIGIRVQDLLDPKILREKI
                     GLAVAEKNV"
BASE COUNT         1850 a         4002 c         4182 g         1763 t
ORIGIN      
        1 ggaggccgag gcgcttcgga aactcgtgag tgcgaacatc ggcgcccatc tcgctccagg
       61 tctccccgag cgcccggatg tcgccgttgt cgacctcgcc gtcgttcgcg ttcgtgacga
      121 cgacgaccga gcccgcgcgc gcgggcaccg agtccgccgc ctcacgcacg gccttgccga
      181 acttgtacac ggcggcgagc gcgcgtgtgg actcgccgtc gtaggaatgc gagggcgtcg
      241 acctgcccgg cagcgagatg ttcggcagcc tgttgaagag gttgacgaag aggaagtcga
      301 cgacgcccgg catcttgggc agcgtcaagg cgggcgccac ggcgacgacg cgcccgacgt
      361 cctcgcggtt ctgggcgatc caggtcgcaa ccgagccgcc catcgagaaa ccgagcacgc
      421 gcacgtcctt tcccaggccg gccgccaggt cgaccacgtc gtcggcgtag tcgcgcagat
      481 cctctgggct caggtcgccg acgtttccta cgccgccgat tgccttcccc gatccgtcgg
      541 ccaggccgtg atggggggcg cgggggatga ggacgttggc gcccgtcgcg tggatgcggt
      601 ctccgaggtc gaggaactgg cgcggacagt tggtcagtcc gtgcacgagc acgaccgcga
      661 cgtcggtctg ctcaccgtgg tcgagcaggc gcgacttgca gggctcgtat acgcgcttcc
      721 gctccccggc gcggatcttc ttgaagcgag agacggcctc ttcgtatccg gaggcgggat
      781 tcggctgcga ccagaacccg tcggtggaag acggcacgag tgagagtacc gcaaggacgg
      841 cgatgacgag cgcgagcagt gccccgccca gccgcacggc gcctccgagc ttcgagtgcc
      901 gctgccgcga accgcgggcc atggcgggca atcatcgcag ctaccctcgg tggccgtgcg
      961 gcgtgcgttt gcagcggttt tcgtggcggt gctggccatc gtcggtgccg cggacgcgcc
     1021 ggcggcgaag aagctgcgcg tgaccgtcgt cgccgactcg gtcggcgcgt cgctgaacta
     1081 tgtcgacgct gcgcgcaagc tcctggcgcg cggccgggtg cttacgctcg acctgaaggt
     1141 gtgtcggcgg ctcgtgcagc ccagctgcac ctatcagggt gccaggccga cgaatgcgct
     1201 cccagccgtc cgggcccacg gacgcaggct tggcgacgtg ctcgtgatca aggtcggtta
     1261 caacgagagc gcgaaggggt accgggcggg aatcgaccgg gtgatgcgcg cggcgctcgc
     1321 gaacggagtc gaggccgttg tctgggtcac cctgcgcgag cagcgcgaca cgtaccgctg
     1381 gacgaacatc gcaatccgct cggctgccaa acgctggaag cggctcacag tgtcggactg
     1441 ggcccgttac agcagtggga ggccgtggtt cgcgtcggac gggctgcaca tgacgcccct
     1501 gggcgcccgc gccctggcgc gctttctcaa cgcgagcatc cgagcggcag cgccgggcta
     1561 gccggccccg taactcgtgg aaagtcgaga gcacctcgct cgccgcgacc gcgtcgtcag
     1621 cgcacgcgga gtcacgaagc gctacggctc gacggtcgcg ctcgacggcc tcgacctcga
     1681 gatcggtccc gggatcaccg gcctgctcgg ttcgaacggc gcgggcaaga cgacgttcat
     1741 ctcgctcgcg ctgggcatcc ggacgcgtga cgcgggcgag ctggtcgtcc tcggcgagga
     1801 tcctgctcag gcgggcatcc acgtccgcgc ccgaatcgga tacgcgccgg agcaccacga
     1861 cctgcccccg gacgtccagg ccgccgatct cgtgcgccac ctggcagagc ttcacggact
     1921 cccggggcag gcggcgatcc agcgggcgaa cgacgctctc tggcaggtcg gcctggggga
     1981 ggagcgcttt cgtccgatcg gcacgatgtc gacgggccag cgccagcgcg tgaagctcgc
     2041 cgctgcgatc gcgcacgacc cggaactcgt gctgctggac gagcccaccg acggcctcga
     2101 cccggtgcag cgcgacgaca tgctcgcgct gatccgccgc gtcgggaccg agttcggcat
     2161 ggacatgctg atctcgtccc accacctgga ggaggtcgaa cggatctgcg acggcgccgt
     2221 gatcatcgac gcgggaaccg tgatccgttc gggctcgctc gcggagctgc gccgaggcga
     2281 ggacgggctc gtcgtcgagg tggacgatcg tgccgcggag ctctgcgcgc tcctgcgcga
     2341 cgcggggctg gaggcgtctg ccgaggggac gctcgtgctc gtgtcgggca cggacggagt
     2401 gcacgacacg atccgagacg ccgtcgccga gctcggtgtg gggctgcggc gcctcgagcc
     2461 ccgcagccgg acgctcgagg acgtgtacct ggaggcgacg tgagtaccga ccgggcccgc
     2521 atctacgacg tcggctatcg ctcctacggc gggccccggc gcgccaccgc ctgggccatg
     2581 gtcacgctgg gcaggcacac gatccagcgc gtgctcggcc tccggcgcgg gttccgtcac
     2641 aagatcctgc cgctgctggc gcttctcgtc tcgttcgtgc cggcgatcgc gtatgtgagc
     2701 ctggccgtcc tgctccccca gagcctgttg gaggaggaca tcctgatctc ctacggcgag
     2761 tactacttct tcatcggaac cgccctcgcg ctcttcgccg cgttcgtggc tccggaggcc
     2821 ctgtgcacag accggcggac cgggatgctc gacctctacc tcgccgggcc gctcgatctg
     2881 aagcgctacc tcggcgccaa gtggctcgcg gtctacgtcg cgatgtcgct catgacggtc
     2941 gggccgctgt tgttcatgct gatcgcgtac tcgatcgagg acgccgggcc gacatggggc
     3001 gagctcccgc tgctcctcct gcggatcgtt gctgccggcg ccgtcgtcgc gcttctctac
     3061 gcagctgtct cgctcggggt ctcgagcctc accgcccgga agggcgtcgc ggcgatctcc
     3121 gtcgttctcc tgctgttcgt gccgagtatc gtcgtagggg tcgcgaccga gagcgccgac
     3181 gcccccgacg agcttgcgat cgcggtagcg ccgtccgtcg ccgccgagct cccggtgcgc
     3241 atcttcggcg aggagagcga gggcgacggc gccgcacaac agctcgcgac gggcctcatc
     3301 gtggcgggat ggcttgtgtg ggtcgccgcc ggagcgctgg tctgcttgtt cgcctaccgg
     3361 cgcttggagc ggcgacggtg agcgggccgc tcgtcaaggc cgacggcgtc tcgaagtggt
     3421 tcggcggggt ggttgccgtg tccgacgtaa gcttcgacat cggcccggga gtcaccgccc
     3481 tgctggggcc gaacggcgcc gggaagtcga cgatgctgcg gatgctgtgc ggcctcgcgc
     3541 gcccctcgaa agggagcgtc tcggtgctcg gacgcgatcc acgctccgac gtcgccgtga
     3601 cgagggcgat cgggctcgcg ccccaacagg agtcggtctt cgagccgctc accgccgcgg
     3661 agttcgtccg gctgatcgct gtcctccacg gtgtcgacga tcccgccggc gccgccgcac
     3721 gggcgctcga gacggtcgaa ctcgatcccg acgacacgcg ccgcctgccc acctactcga
     3781 aaggcatgcg gcaacgcgtg aagctcgccc aggcgatcgt ccacgacccg caggtgatcg
     3841 tgctggacga gccgctcgcc gggttggatc cccgccagcg cgtgcacatg atccggctgt
     3901 tccatcgcct cggcggcgag ggtaggtgcg tgatcgtctc gagccacgtg ctggaggagg
     3961 tcgagcggtt cggctcggac gtgctcgtgc tgtcgagggg ccggctggct gcgtccggcg
     4021 actaccgcga gatccgcgcg ttgatggacg accgcccgcg gcgcgtgcgt gtccgcacgg
     4081 accggcccca tgagctcgcc ggagcgctct tgacgcgcgg tgtcgtggtg ggcgcgaggg
     4141 tgcaggacga cggggcgctc gtgctcgatg ccgaggacgc tggcaggctg gcccacctgc
     4201 tggcgccgct cgccagagag cacgctgcca gcctgctcga agtgcgctcg gtcgacgagg
     4261 atctcgaagg cgtgtttcgc tatctggtgg agcggtgacg gcgacgccga cgacggttcc
     4321 tggggggcgg ctcggacact cttccccgtt ccgggtgctc ttcaggctgc tgctgcgcat
     4381 gcagttgact cccgtgcgcg tcgttgccat ctctgccctc gggctgctgt cgatcgggct
     4441 cgccgggctc gcccgggccg acgacgatcc gcttcgggcg acggtcgagg gcgccgccgc
     4501 atacggcctg ggcctcgtgc tcccactctc cgtgctgtgg ctggcgacgt cgagcatcgg
     4561 agacctcgtc gaggacaagc tgctcgtgta cgtctggctc aagcccgtgc cacgctggca
     4621 gctgcccggt gcggcgatcc tcgcgacggt ggccatcgtc ctgcccctcg tcgtcgctcc
     4681 gctcgtcgtc gcggtcgtcg tggcggggca agagctgctc gtcggggcga cgctgctcgc
     4741 gtccgcgctt gcggcgctgg cgtacggcgg gctcttcgtc gcggccggga tgtggctgcg
     4801 ccgagccctt tggtgggggc tcctgtacat cctcgtctgg gagaacggga tcgcccgcgc
     4861 cgcagacggg gcggcccgtc tctcggtggc gagctatacc cagtcgatcc tggccgacgc
     4921 gggtgacgtc gacttggcac tcggcgggcg atcgctcgtc gcgtcgatcg tcgtgccgct
     4981 cacggtagcc ggcgccggct gcgccctggc cgtgtggcgt taccggacgg cggacgtcga
     5041 ctagatcgcc tctgccaccg gggtcccatg taccgacccc ttgcgtagga tgagcgcgac
     5101 actcgggaag acgagcaccg agatcattcc cgcgccgacc agcgctgcgg ccacgtctct
     5161 cgagagatca ccttcgcgaa cgccgatctc ggtgatggcg acgatgagcg gcagggaggt
     5221 ggcggagaag aaggccagcg agaaacgccc cgcccggtcg agatcgcgac ggtagaggat
     5281 gagcgcggga aggcctctcg cagcgagcag aacgagcgtg gcgaccggca gcagaacgag
     5341 cgccgtggtc gactcgagca gtgcatccag gtcaaagcgc atgccgctga cgacgaagaa
     5401 catgggtacg aacaggccgt agccgacggc ctcgatgcga tcgcggacga tcgttcccgt
     5461 ttcgccccgc gcgacgagcc caacgaccag gcccgccgcg agtgcgccga ggatgaagtc
     5521 gacatcgagt ctcaccgcga tccacaccat gaggatgagc gtgaagacgg agaggcggac
     5581 ggcaagctgg ccgctgcggt gcaccgtctc ggcgatcgtc ctcagcacgt gcggtggccg
     5641 tgcccgaagc gccacgatcg ccgcgccgac gcaaacgagg aagaaggcga ccaggagaac
     5701 gaactcatgc gcagcgctgt agtcggcgct gaggaagact gcaatgaaga tgatcggaaa
     5761 cagctcgccc gcgacacccg ccccggtcgc actcgtgccc aggcgtgtcc cgctcagccc
     5821 gttgtctcgc agcacgggtg tgaggacgcc gagtgtggtc gtcgcgaggg cgatgccgac
     5881 gacgacggga ccgctcagga cgtcggccgc gaagaggagc agggctgcgg cgacggcgat
     5941 cgccgcggag ctgaaccagc cagccgtcgc gaggacgcca agtcgcccgc gtacctgggc
     6001 agcctcgatc tcgagaccgg cgaggaagaa gaggaaggcc aaaccgatgt ccgagaagaa
     6061 gacgatgaac tcgtcgacct gggcgagatc aagcacctga gggccgatca ggatcccgag
     6121 gaacacctct acgacaaccg tcgggacgac gatccttcgg gaggcgcgtg ccagcagcgg
     6181 agcggcgacc gccgcggcaa gaatcaggac gaatgagccg aggtcaggtt cgggcccggt
     6241 cggcatcggc ggcttggtcg atcaggacga cgcgggcttg tgctcgaccg tcgcctcgtc
     6301 cgcgaccggt ggcaggcggg tgacggccgt ctcggactcg gccgcgctct cgatctgcgt
     6361 gtgctcgacc ggcggtgaga accacgccgg gtcggccgcg gccggcgcgg cgggaaccgg
     6421 cggcggcgcc aggagcatct cctcccgcct tcgctccgct cggctcgccg cccactcggc
     6481 gagcgccacg atcacccatg cacccgccat cgcgagggcg atcacgtacg gctcgagatc
     6541 ggcggccgcc gcaagcgccg ccacggcgac gaggaaggca gcctcgagca gaaagcgcgg
     6601 tagcgccggg cggcgccgga gccgtggctc gagcgacccc acccacgcat acgagggagc
     6661 ctcgtcgggc cgatcgtcac ctgcgaccga cggcaacggt ccaccgccca gccgcccgac
     6721 ttcgcgctgg agtgcggcga gctgctcgtt caggcgatgc aactcgtcgg cgacgccgtt
     6781 cgccgcgtcc tcctgctcag tccgctgcgg ctcgtcggcc acgctatcga tcgccctgct
     6841 cgtgcatcag gtcgagcgcc tcctgggctt tgctgtgcac acgcgcgcgg cgctcctcca
     6901 gcgagggccg cttcgcctcc ggctcgacac ctcccacctc gcgggtcgag tcgagcgtcc
     6961 tgcggagctc ttcggcggga tcgggaacgg gctcgagctc ggccccgggc cgccggcgcc
     7021 gacgggcagc ccgcgccagc gcggccgcca gcgcgatccc tcccgcgagg aacaaggtcc
     7081 gtcgcatggg gcgatgctac cgcgctcgcc tgcgggcacc cggcgcactc gggttcgctg
     7141 ctacactccg gccgcttcct gccctgcctc gtgccgcccg ttcccggcgg cgcatcacag
     7201 gcggggccgc cttagaccga gggaaggaga gtaggggacg tgaccgagta cgagatcctg
     7261 ttgctgctcg agcccgagct gagcgaggag gcgcaggctg acgtcgtcgc gcggacgcgc
     7321 gcgctgctcg agaagagcgg cggttcgctc gatcggcacg acgtctgggg acagcgcaag
     7381 ctcgcctacg agatcgacca caaggacgag ggctggtacc acctgctcct gctcacctgc
     7441 tcggccgaga cgctagagga ggtcggccgt gtgctcagga tcgacgacgg cgtgatgcgc
     7501 cacatggcga cccgccggta cgagacgagt cccaccgagc ccctagcggt cggcgcgtcc
     7561 gccagccagg atgccgggga gacccagccc gcagcagagg aggactagcc catggccgcc
     7621 atcaacagcg tcacactcgt cggcaacctc acacgcgacc ctgagctccg gcatacgcct
     7681 tccgggacgc ccgtctgctc gctccgactc gctgtcaaca cgcgacgcaa ggacggcgcg
     7741 agcgggcagt gggtcgagaa gcccaattac ttcgacatca ccgtctgggg ccagcagggc
     7801 gagaactgcg cccagtacct cgccaaggga cgccaggtcg gcgtccaggg caggctcgag
     7861 tggcgtgagt gggagaccca ggagggcgcc aagcgtcagg cggtggaggt cgtggccgac
     7921 accgtgcagt tcctcgggag tcgcggcgaa gccgaaggcg gaggtcagta cgtgccggcg
     7981 gcggccgcgg cgcagacacc cgatgccgac ttctcgtcgg ccggcgacga cgatatcccg
     8041 ttctagatcg gctgaagtga gaaatggctc agaagacaca gcgaaggcgc cagagcccca
     8101 gcaccgctcc gggtcgccgg aagagctgcc acttctgccg cgacaaggtt gccgacgtgg
     8161 attacaagaa cgccaatcag cttcgtcggt acgtctccga gaagggcaag atccgtaacc
     8221 ggcgcatcac cggagcctgc cggcggcatc agaagcaggt cgccgtcgcc gtcaaacgcg
     8281 cccgcgagat ggcgctcctg ccctatgtgg cgagctagcc gatgcaagtg atcctgctca
     8341 cggacgtcga gaaggtcggc ctgcgcggcg aagtggtgaa cgtcgcccga ggctacgcac
     8401 gcaacttcct gctgccgcgg gggctggcag agacggcaag tcccgggcgc gtcgccgagt
     8461 tgcgccgaat cgaggcggag cgcgcgcgcc acgaggcacg ctcggccgac caggcggatg
     8521 agatcgcaga gatcctcggc aagaccgtgc tgcgcttcga cgtcaaggca gggccgaccg
     8581 gggtgctctt cgggtcggtc acgccgacgg acatcgccga cgagatctgg cgcgcgcgga
     8641 aggtccgggt cgaccgtcgc aagatcgggg tcgattcgat caagcgaatc gggcgctatg
     8701 cggtggagat cgacgtctac gagggcgtga tcgccgaggt gaagacgctc gtcgtgccgg
     8761 agggcggcga gctgcctccc gaggaggagc tcgcggcaat ggcggaggag caggcggcag
     8821 aggaagccgc tgccgcggag gccgccgtcg aggagcacga gcaggccgaa gtggccgtcg
     8881 cagaggcgat cgaagtgctc gaggccgaag atgcagaaga agcagaagag gcagacgaag
     8941 cagaagaggg agtagaagcc gccgaagcag atgcgggcgg cgagcccgag cacacggccg
     9001 cagccccggt cgccaccgcc gagagcccgg aagagccgga agcggcggcg ggcgaagagc
     9061 cggtggacga gccgacggcg ccgcccgcgg cctgatcgtc agctgcgagc gcgcgccgcc
     9121 taaccacctc gccgcctcgg cccttccacg gcctttcccc acaggtgctc ccgcctgttg
     9181 agaggagcct gaactctccg ctcgcagcgg cgtggctgtc cccaggacgg aaacgcgttg
     9241 tccccaggag ccgaccgatc gccgtgagtc agcgtggaga accgtccggt gagtgtggaa
     9301 tactcagtcg aacatatgtt cgctcaacga ggacactagc ctgctctcgg gtcgactggc
     9361 gcgcgacctt cgaacgtcgc agagcgcagc gcgcgtggat tcgccggctg ttgacagacg
     9421 gcccgtccac agctccacgc cggccgacga agcaggtttg atggggccat gaccgagctc
     9481 gccgagccgc gcacgagcgc gcacgtcccg ccccagaatc tcgaggccga ggagtccgtc
     9541 ctcggtgcga tgctcctgtc cccgacggcg atcggggtgg tcaccgagat cctctccgcg
     9601 gaggacttct accgcgagag ccacggcacc atctaccgcg cggctctctc gctctggggc
     9661 aagggtgagc ccgtcgacgc catcacgctg gcggacgagc tcgacggccg cagcgagctc
     9721 gagtccgtcg gtggcgcggc gagaatcgcc gagcttgcgg cgctcgtccc ggcggcgtcc
     9781 aatgtggagc actacgcccg gatcgtgcgc gagatggcga ccctcagagg gctcgtccgc
     9841 gtcggcaccg agatcgcgcg gctcgggcgt gaccgccccg gtgagacgcc tgatctcgtc
     9901 gaccggtcgg agcaactcgt cttcgagctg gcgcaggagc gcgtcaccac ggacttcgcg
     9961 cggatcgacg tcctgttgcg ggaggggttc gagcgcatca cccatctcta cgagcagggc
    10021 gcggagctga ccggggtgcc gtcgggcttc cgcgacctcg actcgctcac atccggcttc
    10081 cagcccggaa acctgatcat tctggctgcg cgtccgtcga tgggaaagtc cgcactcgct
    10141 ctctgcgccg cggcgaacct cggcgtgcgc gaggaaattc ccgttgcgct cttcacgctt
    10201 gagatgtcga aggccgaggt cacgcagcgc atgatgtgca gcgaggcaaa ggtggagtcg
    10261 cagcgactcc gctcgggcaa gctccatccc gacgactggc ctcgcctcac ggcggcgtgc
    10321 gaccgcctga tgaaggcacc gatcttcgtc gacgacagcg gctcgatcac catgatggag
    10381 ctccggtcga aggcgcgccg actcaagtcg cgcgagagcg acctcggcct gatcatcgtc
    10441 gactacctgc agctgatgac gtcgggagcc tcggtcgaga acagggtgca ggaggtgtcg
    10501 cagatctccc gttcgctcaa ggttctcgca cgcgatctcg acgttccgat tctcgcgctg
    10561 tcccagctct cgcgcgcagt agagcagcgg cacgacaagc gcccgatcct ctccgacctc
    10621 cgcgagagtg gttcgaacga gcaggatgcc gacctcgtct tcttcgtgta ccgcgacgag
    10681 tactacgccg gcgaagagtc ggatcagcag gggctcgccg aggtgatcct cgccaagcac
    10741 cgcaacgggc cgacggacgc cgtcaagctg agctttctca agcggtacgc gaagttcgcc
    10801 gatctcgcag cgtgacccga gacgccatgg cggagttccc gacccccgag gtggcgctca
    10861 ccgacatcct cgtcgtcgcc gacctcggcg gctcgctgga ctggtatacg cacgtgctcg
    10921 gtgcccgcct cgaacgggag tacggcggaa cctcggccgt actcgagttc cagggcgtct
    10981 ggttgttgct ggtgactgaa ggcggaccga cgccggacaa gccgagcgtc ctcttcgcgc
    11041 cccctgcaga ccccgacctc gtcagtcatt ccttcaccat tcgtgtgccg gactgcgagg
    11101 ctgcctacag gacgttgaaa gcgcgaggcg cggcgtttct cacgcccccg ctcgatcgtg
    11161 ggtcggaagt gcggtgcttc ttccgcgatc ccgatgggca cctcttcgag ctgagcgaat
    11221 cgcgttgacg ccgcgggcag cgtcggcccc cgggcccgcg ggccgagtct tctgcgaaac
    11281 tgagacacgt gccagcgacc gtgatcgtgg gtgcgcaatg gggcgacgag gggaagggga
    11341 agatcgtcga tctcctcgcg tccgagtcag acctcgtctg tcgctaccag ggcggcccga
    11401 atgccggcca caccatcgtc gcggaggggg agacgtacaa gattcgacag atcccgtccg
    11461 ggatcatccg cggcaaggcg agcgccatcg gcgccggctg cgtcgtcgac cccacagtcg
    11521 tgattggcga gttggacgag ctcgagtcac gggggatctc gaccgcgggg ctcgtcttcg
    11581 tttcgggcaa cgcgcatctg atcatgccct ggcacgtcgc gctcgacggt gcgagggaac
    11641 ggcggctcgg gcggttgcag atcgggacaa cgcgtcgggg catcggcccc gcctacgcgg
    11701 acaaggcgac ccgaatcggc attcgcgttc aggacctgct cgacccgaag atcctgcgcg
    11761 agaagatcgg tttggccgtg gccgagaaga acgtctg
//
LOCUS       DBNGNK010000114         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1523964,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000114 DBNGNK010000000
VERSION     DBNGNK010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1523964"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1427
                     /locus_tag="AC150V_03265"
     CDS             69..1427
                     /locus_tag="AC150V_03265"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011632657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR3761970.1"
                     /translation="MLSRVLVANRGEIAVRVIRALHELGLEAVAVFSTADRDAQHVKL
                     ADQAVCIGPSPAVESYLRVSHVIAAAETTGCQAIHPGYGFLSENPAFVEACAANDLVF
                     IGPSAEVMNLLGDKVRAKAALGAAGVSLVPGSEAVDSYADAKAAATELGFPVLVKAAA
                     GGGGRGMRVVSSDAELESSFGAATVEAEAAFGSGSLYLEKAITPARHVEVQVLCDAHG
                     GVATLGERECSIQRRHQKLIEESPSPALTAETRDELERSVEDACRRLGYLNAGTFEFL
                     VGPDGRPSFIEVNCRLQVEHPVSELVTGCDIVREQIRIAAGEQLAVEGRAPRRGHALE
                     IRLNAEDPARGFVPVPGRVTRFRPPLGPGVRLDTAVVEGTEISAHYDSLLAKLIVWDE
                     TREAVIARAELALEEFVVDGVTTTRELALDVLRSPGFRAGEYTTSYLDEAEAQLPSLV
                     PA"
     gene            1424..1810
                     /gene="nusB"
                     /locus_tag="AC150V_03270"
     CDS             1424..1810
                     /gene="nusB"
                     /locus_tag="AC150V_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3761971.1"
                     /translation="MSPSVGRRESRRTALFLLYQWDLTGKPLASLYEGKPDEFAMSLA
                     TEVSSRAGSLDEQITEAADEWTADRLGTLERNILRIGVYELEEATVPREVAINEAVVL
                     AKRYATEDAARLVNGILGRIAREVEA"
     gene            1807..2004
                     /locus_tag="AC150V_03275"
     CDS             1807..2004
                     /locus_tag="AC150V_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761972.1"
                     /translation="MSSNESLQRAEELLERMRVKLEELEQLAETGDADAAVDDLAEIA
                     EIAKEIEAEVTRARTSADAGS"
     gene            1991..2761
                     /locus_tag="AC150V_03280"
     CDS             1991..2761
                     /locus_tag="AC150V_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3761973.1"
                     /translation="MRAPDELRELANAYLRELALTPELGGLETSMRYTLEAGGKRIRP
                     VLCLATTEAAGGDVDAALPAAAALELVHTFSLVHDDLPSMDDDAERRGRPSAHVAFGE
                     GIALLAGDALLAEAFRLALAYETPAIARELAAATLGMIGGQFKDITGEDADLEALHRL
                     KTGALLAGSVACGLWVADVPQGKQAPWRAFAAELGMLFQVVDDVLDGDGYAERLGEDE
                     ARALALATADRASGLLDVIDADTSVLREIVGALAVRTA"
BASE COUNT          456 a          910 c         1010 g          444 t
ORIGIN      
        1 cgagtacggc cagccgctct tcgagctcga gcccgtcgcg ggcccgccgg tcgtctaagg
       61 ctggagtgat gttatctcgc gtcctcgtcg cgaaccgggg ggagatagcg gtgcgggtga
      121 tccgcgcgct gcacgagctc ggtctcgagg ctgtagccgt cttctccacg gcggatcgag
      181 acgctcagca cgtgaagctg gccgatcaag cggtctgcat cgggccgtcc ccggctgtgg
      241 agagctacct ccgcgtctcg catgtgatcg cggccgcgga gacgaccggc tgccaggcga
      301 tccatcccgg ttacgggttc ctgtccgaga acccggcctt cgtcgaagcc tgcgccgcga
      361 acgatctcgt cttcatcggg ccgagcgccg aagtgatgaa cctgctcgga gacaaggtgc
      421 gggcaaaggc ggcgctcggc gccgcggggg tttccctcgt gccgggctcg gaagccgtcg
      481 actcgtatgc cgacgcgaag gccgccgcca ccgagctcgg gttccccgtt ctcgtgaagg
      541 cggccgcagg aggtgggggc cgagggatgc gcgtcgtgtc gagcgacgcg gagctcgagt
      601 cgtccttcgg agccgcgacg gtcgaggcgg aggcggcctt cggcagcggt tcgctctacc
      661 tcgagaaggc gataacgcca gcgcggcacg tcgaggtgca ggtgctctgc gatgctcacg
      721 gtggcgtcgc cacgctcggt gagagggagt gttcgattca gcgtcgccat cagaagctga
      781 tcgaggagtc gccgtctcct gcgttgacgg cggagacgag agacgagctc gaacgatcgg
      841 tcgaagacgc ctgccgccgc ctcggatatc tgaacgccgg tacgttcgag ttcctggtgg
      901 ggcccgacgg acgcccgagc ttcatcgagg tgaactgtcg ccttcaggtc gagcacccgg
      961 tctcggagct cgtgaccggc tgcgacatcg tgcgcgagca gatccggatc gcagccggtg
     1021 agcaacttgc ggtcgaaggt cgcgcgccgc gccggggtca tgcgctcgag atcaggctca
     1081 acgccgaaga tcctgcacga gggttcgtcc cggtaccggg cagggtcaca cggttcagac
     1141 ccccgctcgg gcctggcgtc cggttggaca ccgcagtggt cgaggggaca gagatctcgg
     1201 cccactacga ttccctcctc gccaagctca tcgtgtggga tgagacacgc gaagctgtca
     1261 tcgcgcgagc cgagctggcg ctcgaggagt tcgtcgttga cggcgtgacg acgactcgcg
     1321 agctcgctct cgacgtgctc cgctcgcctg gtttccgcgc cggcgagtac acgacgagct
     1381 acctcgacga ggcggaagcg caacttccgt cgctggtccc ggcatgagcc cctccgtcgg
     1441 ccggcgcgag tcgcgtcgaa ccgccctctt cctcctctat cagtgggatc tgacgggcaa
     1501 gccactcgcg tcgttgtacg aaggaaagcc tgacgagttc gccatgtcgc tggccaccga
     1561 ggtgtcctcg cgagccggtt cactggacga gcagatcacg gaggctgccg atgaatggac
     1621 cgccgaccga ctcggtacgc tcgagcgcaa tatcctgcgg atcggcgtct acgagctgga
     1681 ggaggcaacc gtgccacgcg aggtcgcgat caatgaggcg gtcgttctgg cgaagcgtta
     1741 cgcaaccgag gacgccgcgc gcctcgtgaa cgggatcttg gggcgcatcg cgcgggaggt
     1801 ggaggcatga gcagcaacga gtcgctccaa cgtgccgagg agctgctgga gcgaatgcgg
     1861 gtcaagctcg aggaactcga gcaactggcc gaaaccggtg acgcggacgc cgcagtggac
     1921 gacctggccg agatcgccga gatcgcgaag gagatcgagg cggaggtcac ccgtgcgcgg
     1981 acgagtgcgg atgcgggctc ctgacgagct tcgcgagctc gcgaacgcgt atctccgcga
     2041 gcttgccctg acgcccgagc tcggcgggct ggagacttcg atgcggtaca cgcttgaagc
     2101 gggcggcaag cgcattcggc cggtgctgtg cctcgcgacc acggaagcgg ctggcggcga
     2161 cgtcgacgcg gcgctgccgg cggccgcggc gctcgagctg gtgcacacgt tctcgctcgt
     2221 gcacgacgat cttccgtcga tggacgacga cgcggagcga cgtggtcggc cgagcgctca
     2281 tgtcgccttc ggcgagggaa tcgcgctgct cgcgggcgac gcgctcctcg ccgaggcgtt
     2341 ccggctcgcg ctcgcgtacg agacgcccgc gatcgcccgc gagctcgccg ccgcaacgct
     2401 cggcatgatc ggcggccagt tcaaggacat caccggtgaa gacgctgatc tcgaggcgtt
     2461 gcatcgcttg aagaccggag cgctcctcgc aggatcggtc gcctgcggcc tctgggtggc
     2521 cgatgtgcca cagggcaagc aagcaccgtg gcgcgcgttc gcggccgaac tcggcatgct
     2581 gttccaagtc gtcgacgacg tgctcgacgg cgacgggtac gccgagcggc tgggtgagga
     2641 cgaggcgcgc gcgctggccc tcgcgacggc cgaccgtgca agcgggctgc tcgacgtgat
     2701 cgacgcggac acctcggtcc tgcgcgagat cgtcggagct ctggcggtgc gaacggcctg
     2761 aagagccagg tcagacgggg caccccaacc caccacccac tgacgatcgt aacgacgaat
//
LOCUS       DBNGNK010000115         4870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_520549,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000115 DBNGNK010000000
VERSION     DBNGNK010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_520549"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC150V_03285"
     CDS             complement(<1..377)
                     /locus_tag="AC150V_03285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR3761974.1"
                     /translation="MSKLPPVASRLDGGLGLALEAAADGVATLRLDPTPLSVVDDEGP
                     PFLHGGALATCVDTAAWYAAASAGGGAWVVSGLHVDALRLAGPEPHRVTARSVKVGRT
                     LAVVDVESVPLDDPERVVAVGRAT"
     gene            complement(383..1522)
                     /locus_tag="AC150V_03290"
     CDS             complement(383..1522)
                     /locus_tag="AC150V_03290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042779.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease"
                     /protein_id="HIR3761975.1"
                     /translation="MEFGLSSVRVRNFRSAREIELQASPMNAFIGQAGAGKSNVLRAI
                     RALLEPDFELAPRDVTAGETELSLEGRLASGETISLSDRTGAPPVWTFPARLRDAELV
                     ATDPVPSVFADALASDIAPRIALVHALEACARDVTGVVFLIEEPELFLTPQGHRYLYR
                     LLRGIAARGNQVFISTHAPGFLNVARLDEIHRVSRDAQGVTTVDRLEPVRVDDEFRAM
                     CEFDAERGELLMSRAAVLVEGMTEKLAFPYVFAALGHDVDRLAISIVECGGKTNLPLF
                     VEICRRADVPCVAVYDTDIPPGKKGNAGAARLNEMIAEAAGAERVVSLEPDFEGALGI
                     VKKGHKPERAFRVLTDMRADDLPPPFRHLVDLTLEIARPEPPTYS"
     gene            1607..2503
                     /locus_tag="AC150V_03295"
     CDS             1607..2503
                     /locus_tag="AC150V_03295"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR3761976.1"
                     /translation="MTEITAADAAALARHFRVEPGTKVKLADHDTRDSLGFKKPPDAK
                     ELLREGTEVLVEYQRRLVAQDSDSLLIVFQAMDAAGKDGAIRHVMSGVNPQGVRVTSF
                     KAPAGVELEHDYLWRHMVALPQRGMIGIFNRSHYEEVLVVRVHPEILENRKLPDALKD
                     RGIWKRRFDQINAFERYLVDNGHHVVKIFLNVSKREQRKRFLSRIDEPEKNWKFSAAD
                     IRERGFWDAYQEAYEDAIRHTSTKWAPWHIIPADRKWFARIAVACVIGDKLREIDPKY
                     PTVSEAHMEELRAAAEELRSEK"
     gene            2552..3184
                     /locus_tag="AC150V_03300"
     CDS             2552..3184
                     /locus_tag="AC150V_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3761977.1"
                     /translation="MKRIATALGVVLGVALLYRLLLREPLLTWGATREEAHGSLPGDE
                     LLEAADLVSTRAIAIDAPPSAVWPWLVQLGQGRGGAYTYDWIENLFGLDMHSADAIVA
                     EWQQLDVGDTIGVNPDGSGLRVAVLEPERVLATRSDDGSWVWTFVLLPEGGGTRLVSR
                     NRFSTENMSVLTRLVLLAFTEPGSWVMERKMLLGIKERAERLTSAETPMS"
     gene            complement(3250..4761)
                     /gene="ilvA"
                     /locus_tag="AC150V_03305"
     CDS             complement(3250..4761)
                     /gene="ilvA"
                     /locus_tag="AC150V_03305"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HIR3761978.1"
                     /translation="MTTDYLEAILNARVYDVAIESPLEEAARLSRRLDNRLLLKREDL
                     QPVFSFKLRGAYNKMARLPRKTLERGVVTASAGNHAQGVALAAERLGSPATIVMPTTT
                     PQIKVNAVRARGAKVVLEGDSYDEAYARAVKLGRAEGLQFVHPYDDPEVIAGQGTIAM
                     EILRQTREPIDAIFVPVGGGGLIAGIAVYTKRLRPGIRVIGVEPDDANAMERSLRAKR
                     RVRLSHVGLFADGVAVRQVGKETFRLARRHVDEMLLVDTDELCAAIKDVFEDTRSIVE
                     PAAALGVAGAKRYVEATGVTGATFVSILGGANMDFDQLRFVSERAELGERREAVFAAT
                     IPERSGSFRAFCKLLGKRNITEFNFRFDDPREAHVFVGVQIHDAAETEEILALLEGKG
                     IPAIDLTDNEMAKLHIRHLVGGRSDDVTDEVLYRFEFPERPGALMRFLTEIGSTWNIS
                     LFHYRNHGGDHGRVLAGIQVPHAQRKEFRGFLNTLGYVYEEETGNPAYRLFLA"
BASE COUNT          772 a         1629 c         1718 g          751 t
ORIGIN      
        1 gtggcgcggc cgaccgcgac gacccgttcg gggtcgtcga gagggacgct ctcgacgtcg
       61 acgaccgcga gggtgcgacc gaccttgacg ctgcgcgcgg tcactcgatg aggctccggc
      121 ccggcgagcc ggagcgcatc gacgtgcagc cccgagacga cccaggcgcc gccaccggcg
      181 ctcgccgccg cgtaccaggc ggcggtgtcg acgcaggtcg cgagcgcgcc gccgtggagg
      241 aacggtggcc cttcgtcgtc gacgacggac agcggcgtcg ggtcgagtcg aagcgttgcg
      301 acgccgtcgg ccgccgcctc gagggcgagc ccgagcccgc cgtcgaggcg agaggcgacc
      361 ggcgggagct tcgacacggc ggctaactgt acgtcggcgg ctcgggtcgc gcgatctcga
      421 gcgtcaggtc gacgaggtgc cggaacggag gcgggaggtc gtcggcgcgc atgtcggtga
      481 gcacccggaa agcgcgctcc ggcttgtgtc ctttcttgac gatcccgagc gcgccttcga
      541 agtcgggctc gagcgagacg actcgctcgg cgccggcggc ttccgcgatc atctcgttca
      601 accgggcggc cccggcgttt cccttctttc cgggggggat gtcggtgtcg tatacggcga
      661 cgcacggaac gtccgcgcgg cggcagatct ctacgaagag cgggagattg gtcttcccgc
      721 cgcactcgac gatcgagatt gccagtcggt cgacgtcgtg cccgagcgcg gcaaagacat
      781 acgggaaggc gagcttctcc gtcatgccct cgacgagcac ggcggctcgg gacatcagca
      841 gctcgccgcg ctctgcgtcg aactcgcaca tggcccggaa ctcgtcgtcg acgcgaaccg
      901 gctcgaggcg gtcgacagtg gtcacgccct gcgcgtcgcg gctgacgcgg tggatctcgt
      961 cgaggcgcgc gacgttgagg aagcccggcg cgtgcgtgga gatgaagacc tggtttccgc
     1021 gtgcggcgat cccgcggagg agccggtaga ggtagcgatg cccctgcggg gtcaggaaga
     1081 gctccggctc ctcgatcagg aagacgacgc ccgtcacgtc gcgtgcgcag gcttcgagag
     1141 cgtgtacgag ggcgatccgg ggagcgatgt ccgatgcgag cgcgtcggcg aagacgctgg
     1201 ggacgggatc ggtcgccacg agctcggcgt cgcgcagcct cgcggggaag gtccacaccg
     1261 gcggggcgcc cgttctgtcg gagagcgaga tcgtctctcc cgacgccagc ctcccctcga
     1321 gcgacagctc cgtctcgcct gccgtgacgt cgcgcggagc aagttcgaag tcgggttcga
     1381 gcaacgcacg gatcgcgcgg agcacgttgg acttgcccgc gcccgcctgg ccgatgaagg
     1441 cgttcatcgg cgaggcctgc agctcgatct cgcgggcgga ccggaagttc cgcacccgca
     1501 cggacgacag tccgaactcc acgcccccga ctgtagccag acgctcattg ctgattgccc
     1561 ggatgccgac gtgcgcgcct cgcccgacgg acgatagggt cgcgccatga ccgagatcac
     1621 ggctgcagac gccgccgctc tcgcgcgcca cttccgagtc gagccgggga cgaaggtcaa
     1681 gcttgccgac cacgacacgc gcgactcgct cggcttcaag aagccgcccg acgcgaagga
     1741 gctgctccgg gagggaacag aggttctcgt cgagtaccag cgccggctcg tcgcccagga
     1801 ctccgactcg ctgctgatcg tcttccaggc gatggacgcg gccggaaagg acggcgcgat
     1861 ccgccacgtc atgtcgggcg tcaacccgca gggcgtccgc gtgacgagct tcaaggcacc
     1921 cgccggggtc gagctcgagc acgactacct ctggcggcac atggtcgccc tgccgcagcg
     1981 gggcatgatc ggcatcttca accgctcgca ctacgaagag gtcctcgtcg tccgcgttca
     2041 tccagagatc ctcgagaacc ggaagctgcc cgacgcgctc aaggacagag gcatctggaa
     2101 gcgccgcttc gaccagatca acgcctttga gcgctacctc gtcgacaacg ggcatcacgt
     2161 cgtgaagatc ttcctcaacg tctcgaagcg cgagcagcgg aagcgctttc tgtcgcgcat
     2221 cgacgagccg gagaagaact ggaagttctc ggccgccgac atccgcgagc gtggcttctg
     2281 ggacgcctac caggaggcat acgaggacgc gatcagacac acgagcacga agtgggcacc
     2341 gtggcacatc atcccggccg accggaaatg gttcgcgcga atcgcggtcg cgtgcgtcat
     2401 cggcgacaag ctgcgcgaga tcgacccgaa gtatccgacc gtcagcgagg cgcacatgga
     2461 ggagcttcgc gccgcggcgg aagagctgag gagcgagaag tagccggccg gttcggcgag
     2521 aaccggctcc ggggaagaaa gccgtagcgg catgaagcgg atcgcgacag ctctcggcgt
     2581 cgttctcggc gtggcgctcc tctaccggct cctgctgcgg gagccgctcc tcacgtgggg
     2641 cgcgacccga gaggaggcgc acggatccct tcccggagac gagttgctcg aggcggccga
     2701 cctggtctcg acgcgcgcga tcgcgatcga cgctccgccg tcggccgtct ggccctggct
     2761 cgtgcagctg gggcaggggc gcggcggggc gtacacgtac gactggatcg agaatctgtt
     2821 cgggctcgac atgcacagcg ccgacgcgat agtcgccgag tggcagcagc tcgacgtcgg
     2881 tgacacgatc ggcgtcaacc cagacggctc cggcctcagg gtcgctgtgc tcgagccgga
     2941 gcgcgtgctc gcgacccgct ccgacgacgg cagctgggtc tggacgttcg tcctcctgcc
     3001 agagggcggc ggcacgcgcc tggtcagccg caaccgcttc tcgaccgaga acatgtctgt
     3061 cctgacgcgt ctggtgctgc tcgccttcac cgagccggga tcgtgggtga tggagcggaa
     3121 gatgctgctc ggcatcaagg agcgcgccga gcggctcacc tcggcggaaa cgccgatgag
     3181 ctaggctccc gggcgccgga gcggaagacg ccaccggact tgtcgagcga ttcggctcct
     3241 gagctccggc taggcgagaa acagccggta ggcggggttt ccggtctcct cttcgtacac
     3301 gtagccgagc gtgttcagga atccccggaa ctccttccgt tgcgcgtgcg ggacctggat
     3361 gccggcgaga acgcgcccgt ggtcgccgcc gtggttgcgg tagtggaaga ggctgatgtt
     3421 ccaggtcgag ccgatctcgg tcaggaagcg catgagggcg ccgggccgct cggggaactc
     3481 gaaccggtag agcacctcgt cggtgacatc gtccgagcgc ccgccgacga ggtgccggat
     3541 gtgcagcttg gccatctcgt tgtcggtgag gtcgattgcc gggattccct ttccctcgag
     3601 gagcgcaagg atctcctctg tctcggctgc gtcgtggatc tggacaccga cgaagacgtg
     3661 cgcctcgcgc gggtcgtcga agcggaagtt gaactcagtg atattgcgct tgccgaggag
     3721 cttgcagaac gcacggaagc tcccggaccg ctcggggatc gtcgcggcga agactgcctc
     3781 ccgacgctcg ccgagctcgg cgcgctcgga gacgaagcgg agctggtcga agtccatgtt
     3841 cgcgccgccg aggatcgaca cgaacgtcgc gcctgtgact ccggtcgcct cgacgtagcg
     3901 cttcgcaccc gcgacgccga gcgccgccgc cggctccacg atcgagcgcg tgtcctcgaa
     3961 gacgtccttg atggctgcgc agagctcgtc ggtgtcgacg agcagcatct cgtcgacgtg
     4021 cctgcgggcg agccggaacg tctccttgcc gacctggcgg acggcgacgc cgtcggcgaa
     4081 cagcccgaca tgtgagaggc gcacgcgccg cttggcgcgc agcgaccgct ccatcgcgtt
     4141 ggcgtcgtcg ggctcgactc cgatcacgcg gatcccggga cggagtcgct tcgtgtagac
     4201 ggcgatgccc gcgatcaggc cgccgccacc caccgggacg aagatcgcgt cgatcggctc
     4261 ccgcgtctgg cgcaggatct ccatcgcgat cgtcccttgt cctgcgatca cctcggggtc
     4321 gtcgtagggg tggacgaact ggagcccttc cgcccggccg agcttgacgg ctctcgcgta
     4381 cgcctcgtcg tacgagtcgc cctcgagcac gaccttcgcg ccgcgtgcgc gtaccgcgtt
     4441 caccttgatc tgcggcgtcg tcgtgggcat cacgatggtc gccggcgagc cgagccgctc
     4501 ggcggcgaga gcgacaccct gcgcgtggtt tccggccgag gctgtgacga cgcctcgctc
     4561 aagggtcttc cgcggcagac gggccatctt gttgtatgcg ccccgcagct tgaacgagaa
     4621 gaccggctgc aggtcttcac gcttcagaag cagccggttg tcgagacggc gcgacagcct
     4681 cgccgcctct tccagcgggc tctcgatcgc cacgtcgtag acgcgcgcgt tgaggatcgc
     4741 ctcgaggtag tcggtcgtca tcgtgatgct ccgccgcgtg cggccgagcg tcagaccttg
     4801 cggatctcgt cgccggggcg aaccgtaccg gcctcgacga cgcgggcgta cattccgcgc
     4861 agccgcagcg
//
LOCUS       DBNGNK010000116         2778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1596618,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000116 DBNGNK010000000
VERSION     DBNGNK010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1596618"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="AC150V_03310"
     CDS             complement(<1..833)
                     /locus_tag="AC150V_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761979.1"
                     /translation="MSATRVDSYRSWLIGELVLLVLLGAATAAFALTRDLDGYALPSA
                     RLALDTAVVVLATIVAILAAIRFRVEGRLMDLLVAAGFFGSGLGILAFRVAPLLGGDP
                     LGPEEAWAAIATDVAAAALIAAAPFVHRRVAGRRQALAIAAVSVLLAVPAVWGLTHLA
                     GAGGPVDGPAAAQPITATFAFALLALLALVAIVGFGWRYRRHGRDLDSWFALALTLIL
                     FADLHYLLSPVLASGYVREGDFLRLLSYGVLLVGVWRAISSAEFGRAVAEERARIARE
                     I"
     gene            876..1520
                     /locus_tag="AC150V_03315"
     CDS             876..1520
                     /locus_tag="AC150V_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3761980.1"
                     /translation="MAERILIVDDHPLTADALSSLLTQNGFDVVGIAGDGSEAIERAS
                     DLAPDIVLLDLTMPGMDGLTALPRIREAAPECEVVVLTASGTEENLLGAIRGGAAGYL
                     LKSEPPERIVEFLQGVADGEAALSGQIARRLLDRVRDGGRLGGGVPDEIASRLSAREV
                     EVLLLLDEHLSTDEIAARLFISEHTVRSHVKSLLRKLGVSSRREALEQLELARG"
     gene            complement(1736..2584)
                     /locus_tag="AC150V_03320"
     CDS             complement(1736..2584)
                     /locus_tag="AC150V_03320"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3761981.1"
                     /translation="MELVSHHSMAGLHPTGNHPERPERLIRLLEHFDDVRLGRPAERS
                     AIERVHDAGYVAAVEGLHEETWLDADTVAYGTSWEAALLAAGCSIEAAERDGFALVRP
                     PGHHALRDRAMGFCLFDNIAVAARHAQTELGLGRIAIVDWDVHHGNGTEAIFRGDDTV
                     LFVSLHQWPFYPGTGGPGSGGADTVNVPLPAGSGDDDYAAAFRDVVEPAVSVFAPDLL
                     LVSAGFDAHVDDPLGGMRLTESGFRDVAARCSRLAPRVAAVLEGGYNLDTLPALVEAA
                     REGFAS"
     gene            complement(2584..>2778)
                     /locus_tag="AC150V_03325"
     CDS             complement(2584..>2778)
                     /locus_tag="AC150V_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761982.1"
                     /translation="EAILAAGRFPEEADRLRLYLKVATLDADAPLPSLADREPDWLEG
                     ARWAERHGLGRLAGRLEEAA"
BASE COUNT          469 a          938 c          992 g          379 t
ORIGIN      
        1 tggatctcgc gtgctatccg cgcgcgctcc tcggcgaccg cgcgtccgaa ctcggcggag
       61 ctgatcgccc gccagacccc cacgaggagc acgccgtagg aaaggaggcg caggaagtca
      121 ccctcccgga cataaccgct cgcgagcacg ggggagagca gatagtggag gtccgcgaag
      181 aggatcagcg tcagtgcgag ggcgaaccag ctgtcgaggt cgcgcccgtg tctgcgatac
      241 cgccatccga agccgacgat ggcaacgagc gcaagcagtg cgaggagggc gaatgcgaat
      301 gtcgcggtga tcggttgcgc cgctgcgggc ccgtcgacgg gcccgccggc tccggccaga
      361 tgggtgaggc cccagaccgc aggcacggcg aggagaaccg agacggcggc gatcgcgaga
      421 gcctgccgtc gcccggccac ccgtcgatga acgaacggcg cagcggcgat gagtgcggcg
      481 gccgcaacgt ctgtggcgat tgccgcccag gcctcctccg ggcccagcgg gtctccccca
      541 agcaagggag cgacgcgaaa cgcaaggatg ccgaggccgg agccgaagaa gccggccgcg
      601 accaggagat ccatcagcct cccctcgacg cgaaaacgga tcgcggcgag aatcgcgacg
      661 atcgttgcaa ggacgacaac ggccgtgtca agcgccagac gggccgatgg cagcgcatac
      721 ccgtcgagat cccgtgtcag ggcgaaggcc gcggtggcgg cgccgagcag caccagcagc
      781 acgagctcgc cgatcagcca ggagcggtaa gagtcgaccc tcgttgcact cacaatcccc
      841 ttttcggcag gcggggtgct gccgataagc gttgagtggc cgagcgcatc ctgatcgtcg
      901 acgaccaccc gctcacagca gacgcccttt catcgctcct gacgcagaac gggttcgacg
      961 tcgtcggcat cgccggcgac ggcagcgagg cgatcgagcg agcatccgat ctggccccgg
     1021 acatcgtgct gctcgatctg acgatgcccg ggatggacgg gctgacggct cttccgcgga
     1081 ttcgcgaagc ggcgcccgaa tgtgaggtcg tcgtcctgac cgcttcggga accgaggaga
     1141 acctgctcgg cgcgattcgc ggcggcgcgg ccggctatct gctcaagagc gagccgcccg
     1201 agcgcatcgt cgagttcttg caaggcgtcg ccgacggcga agccgcactg tcgggccaga
     1261 tcgcacgcag gctcctcgat cgcgtccgcg acggtgggcg actgggaggc ggagtgccgg
     1321 acgagatcgc gtcacgattg tcggcgcgcg aggtcgaggt gttgctgctc ctggacgagc
     1381 acctgtcgac ggacgagatc gccgcacgtc tgttcatctc cgagcacacc gtccgctcgc
     1441 acgtgaagag ccttcttcgc aagctcggcg tctcgtcgcg acgcgaggcc ttggaacagc
     1501 tcgaactggc gcgcgggtag tcgcgttccc ccgcgccggg ggagcagaat cgttcacatg
     1561 aggcgatgtt gtcggtcggt accgaggccg ataagtcggg tgacgacgca ggactctcca
     1621 cacgggacgc agagaggatg ccgcaggggc ggccgggaga aaccggccgc cctcctcttg
     1681 tggtaggggg gtttcgccgg tcccccggcc cgctcaggta gcgcgagtgc cgccgtcagg
     1741 aggcgaagcc ttcgcgggcg gcctcgacga gcgccggaag cgtgtcgagg ttgtaccccc
     1801 cttccaacac ggcggcgacc cggggcgcga gcctcgagca gcgcgcggcc acgtcacgga
     1861 aaccgctctc cgtgaggcgc attcctccga gtggatcgtc aacgtgagcg tcgaagcccg
     1921 ccgagacgag caggagatcg ggggcaaaga cgctcacagc cggctcgacg acgtcgcgga
     1981 aggcggcggc atagtcgtcg tcgcccgagc cggccggcag cggcacgttg acggtgtccg
     2041 ccccgccgct tcccggtccg cccgtgccgg ggtagaaggg ccactggtgc agcgagacga
     2101 agagcacggt gtcgtcgccg cgaaagatgg cctcggtgcc gttcccgtgg tgcacgtccc
     2161 aatcaacgat cgcgatcctg ccgagcccca gctcggtctg tgcgtggcgg gcggcgacgg
     2221 cgatgttgtc gaaaaggcag aagcccatcg ctcgatcgcg cagcgcgtgg tgcccgggcg
     2281 gacggacgag ggcgaagccg tctctctcgg ccgcctcgat cgagcagccg gcggccagca
     2341 gcgcggcttc ccacgacgtt ccgtatgcga ccgtgtccgc gtcgagccag gtctcctcgt
     2401 gcaacccttc gaccgctgcg acatagccgg catcgtgaac gcgctcgatc gcgctgcgtt
     2461 cggcgggccg gcccaggcgc acgtcgtcga agtgctcgag cagccggatc aggcgctccg
     2521 gccgctctgg atgattcccg gtcgggtgaa gcccggccat cgagtgatgg gagacgagct
     2581 ccatcaggcg gcctcctcga ggcgtcccgc cagccgtccg agcccgtgcc gctcggccca
     2641 ccgggcgcct tccagccagt cgggctcacg atcggcgagc gacggcagcg gcgcgtccgc
     2701 gtcgagcgtc gcgaccttga gatagaggcg cagccgatcg gcctcttccg ggaaacggcc
     2761 tgccgcgagg atcgcctc
//
LOCUS       DBNGNK010000117        10158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_449702,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000117 DBNGNK010000000
VERSION     DBNGNK010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10158
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_449702"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="AC150V_03330"
     CDS             <1..605
                     /locus_tag="AC150V_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3761983.1"
                     /translation="DIFRNKHTWFMTSLYIMTFGSFAGFSATFGLLIKEVYGDFENAP
                     EPLTYAFLGPLVGSLARVAAGPISDRLTGSFVTQIAGFGLLGSAIGAAFFMSPSSTDE
                     FKWFLLFMLLAFFFSGMGNASTFKQMPMIFPPRQGAGVIGWTAAIAAYGPFFFSVLIA
                     AVISIWGSPTVFFIGIAVFYAINIAINWWFYARRGAEAPC"
     gene            712..1638
                     /locus_tag="AC150V_03335"
     CDS             712..1638
                     /locus_tag="AC150V_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3761984.1"
                     /translation="MEPSQRGRESSRIRRFAVGLVTAVACLSLAGALGSAALAATDDD
                     GEAVFENSGCGGCHAFAPAGSSGTVGPSLDETSLSTDDIVVILTDGKGAMPSFAGQLS
                     PAEIDAVAAYVGSTAEPPPAPEPDTTEEPPPDTTEEPPPDTTEEPAPDTTEEPAPEPE
                     PIAATGDPDRGKSLFEGSDRFENSGPSCLSCHSVAGVGALGGGRLGPDLTNAYEKFGG
                     AQGLRAALETTPWPTMAPVFSNHPLKDDELDDLVAFLETAPQRQEAENSAGKLIGLST
                     AAAVVMVVGGVAIWRRRLPDVRKNLVNRSKGK"
     gene            1648..5301
                     /locus_tag="AC150V_03340"
                     /pseudo
     CDS             1648..5301
                     /locus_tag="AC150V_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
     gene            5328..6833
                     /gene="narH"
                     /locus_tag="AC150V_03345"
     CDS             5328..6833
                     /gene="narH"
                     /locus_tag="AC150V_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HIR3761985.1"
                     /translation="MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGGEYMWWNNV
                     ETKPGTGYPTLWEDQEKYRGGWKQDGDGVRLRLQGKASTLGNIFANPSLPTVDDYYEP
                     WTYDYENLFDAPAGDDQPTARTVSMITGEPMDVEAGPNWDDDLGGSPIYAANDPNLAE
                     VPPEERRQLDELEQMMFFYLPRICNHCVNPSCVAACPAGALYKRGEDGIVLLSQEKCR
                     AWRMCVSGCPYKKTYYNWATGKSEKCILCYPRLESGQAPACFHSCVGRIRYLGVLLYD
                     ASQLERAASVPDDQLVEAHRSLIQDPFDEDVIKAAKENGVTAGMITAAQESPVYRFVK
                     EWKLALPLHAEFRTLPMLFYVPPMLPVIANVENGRYKVDGSNGDVESAVPSLSHIDKS
                     RAPIRYMASLFSAGNEEIVAEVYRKLFAVRVVMRAKKVGNVPDAEVDAALEAGKTTDE
                     EAEDIFRLTSMPTFKERFVLPPLARELQMVDPHKNKQETGFGPRQAPERRW"
     gene            6830..7501
                     /locus_tag="AC150V_03350"
     CDS             6830..7501
                     /locus_tag="AC150V_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HIR3761986.1"
                     /translation="MSTIALTHADRQVVARSFADVLSYPYASPIAVAGASADLVRERS
                     DEAATLLEAFASLVDSAGLGTLQEEYTRAFDLDTMSKTEPTCYPYVGHYLFDESHKRG
                     AFILGLKKRYRATGFEDDSDLPDHLVVLLRFLTVCEDDELAEELVDDAILPALARMAV
                     AAGRPPTEEVSIRGAYIGVLRALELTLSDGRPERVVDPLDREAEREWLRDRDSLGIDR
                     QRCGH"
     gene            7520..8194
                     /gene="narI"
                     /locus_tag="AC150V_03355"
     CDS             7520..8194
                     /gene="narI"
                     /locus_tag="AC150V_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR3761987.1"
                     /translation="MWNTLLFAVLPYVAVAIAIAGGIARYRMDRFSYSSQSSQFLESR
                     ALFWGSVFWHYGILVVLLGHILALMFWDPWAALVSDPDRLYTIEVIGLGVSILALVGL
                     AVLVVRRLAVRRVASVTSPMDIALLVVLAAQVVLGIWVALAYRWGSDWAVFTAVPWMH
                     SLFKLNPKPEFMAALPVVVKVHALGGFLLIALFPFTRLVHIVTYPVTYLWRSYQVVVW
                     NRSRQH"
     gene            8248..9168
                     /locus_tag="AC150V_03360"
     CDS             8248..9168
                     /locus_tag="AC150V_03360"
                     /inference="COORDINATES: protein motif:HMM:NF014542.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR3761988.1"
                     /translation="MLAGFLGSGKTTLLNRILAGGHGMKVAVLVNDFGSINVDSRLIV
                     SRDATMITLDNGCICCSISGDLVSQLTDLLEGPWSPEHVLVETSGVSDPGRLLLAFRH
                     PHLRSLARVDGMITMVDAAGFETIPPTMHELARRQLAGADLIVFNKVDLVAEDALAAL
                     RGRLTYPQARTIDASHGNVPLELVLGIEAPERAPSENGGASSPEHGEAFATWSWRSSR
                     PLALAEVREVLGSLPSNVYRAKGFLYVAEAPAERIVAHVVGRRVDLRPSGWWGDAEPQ
                     TELVFISLEPRIDAVGLRRRLRATTAPSQA"
     gene            9302..>10158
                     /locus_tag="AC150V_03365"
     CDS             9302..>10158
                     /locus_tag="AC150V_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3761989.1"
                     /translation="MPTGPSGSKRAARPPLVVAWEVTSACPLACRHCRATAQVAPHPA
                     ELTHEEGRAFIADLAKDFPGAVLILTGGEPLTRPDTLDLAREAGALGLQVALSVDVGW
                     LLTPERCTAIREAGVRRVSFSIHFPDAEQSDQFADTPGFFMAARSGLENLVAAGVPFQ
                     LHTSVMRANAELLPRIYDLALEIGAAGWELFVLVPTGRGSGLAGEELEPLEQDRVLRW
                     LYDLQRTAPFPVKQICAPHFRRVEAQVARERGERRPERVTTRLSVLSRGCLSGNGFCF
                     VSHVGDVCG"
BASE COUNT         1697 a         3571 c         3297 g         1593 t
ORIGIN      
        1 tcgacatctt ccgcaacaag cacacgtggt tcatgacctc gctctacatc atgaccttcg
       61 ggtcgttcgc cggcttctcg gcgaccttcg gcttgctcat caaggaggtc tacggcgact
      121 tcgagaatgc gccggaaccg ctgacgtacg ccttcctggg gccgctcgtc ggctccctcg
      181 cgcgagtcgc cgccggacct atctcggacc gcctcaccgg ctcgttcgtg acgcagatcg
      241 ccggtttcgg acttctgggt tccgcgatcg gcgccgcgtt cttcatgagc ccgtcttcga
      301 ccgacgagtt caagtggttc ctgctcttca tgctgctcgc gttcttcttc tcagggatgg
      361 ggaacgcgtc gaccttcaag cagatgccga tgatcttccc cccacgccag ggcgcaggcg
      421 tgatcggctg gacggcggcg atcgcggcct acggcccgtt cttcttctct gtgctgatcg
      481 cggcagtcat ctcgatctgg ggctcaccaa ccgtcttctt catcgggatc gccgtcttct
      541 acgcgatcaa catcgcgatc aactggtggt tctacgcgcg gcgcggcgcc gaggcgccct
      601 gctgacagct cgttcgaggg cttccgttgc gcctgatcaa tactgaccac gcccgtccac
      661 ccgggcggag ctagcgtcgg ccaggagatc cagcgagatc ggaggccggc cgtggaaccg
      721 tcgcaacgag gcagagagtc gtcacgaata cgccgcttcg cggtcggcct cgtcaccgcc
      781 gttgcctgcc tgtcgctcgc gggtgcgctc ggctccgccg ctctcgccgc aaccgacgac
      841 gacggcgaag cggtgttcga aaacagcggc tgcggcggct gccatgcctt cgcgcccgcc
      901 gggtcgagcg gaacggtcgg gccgagcctc gacgagacgt ctctttcgac ggacgacatc
      961 gtggtgatcc tcaccgacgg caagggcgcg atgccctcgt tcgcgggcca gttgagcccg
     1021 gcggagatcg acgccgtcgc cgcgtacgtc ggttccacgg cagagccgcc tccggcaccc
     1081 gaaccggaca cgaccgagga gccgccgccg gacacgaccg aggagccgcc gccggacacg
     1141 acggaagagc cggcgcctga cacgacggag gagcctgcac ctgagccgga gccgatcgcc
     1201 gccaccgggg acccggaccg cggcaagagc ctgttcgagg gctccgaccg gttcgagaac
     1261 agcgggccgt cctgtctgtc gtgccactcg gtggcagggg tcggagcgct cggcggtgga
     1321 cggctcggcc ctgacctcac gaacgcgtac gagaagttcg gcggggcgca gggcctccgc
     1381 gccgcgctcg agaccacgcc gtggccgacc atggccccgg tcttctccaa ccatccgctg
     1441 aaggacgacg agctcgacga cctcgtcgcc ttcctcgaga ccgcaccgca gcggcaggag
     1501 gccgagaaca gcgccggcaa gctgatcggg ctctcgacag cggctgccgt cgtgatggtc
     1561 gtgggcggag tggccatctg gcgacgccgc ctgcccgacg tccgcaagaa cctcgtcaac
     1621 cgctcgaaag ggaagtgagg cgccgccgtg ggctggatcc aggacctcgt gaagccgaag
     1681 acccgccgct gggaggagtt ctatcggaac cgctggcagc acgacaccgc ggtgcgctcg
     1741 acccacgggg tcaactgcac cggcggctgc tcctggatgg tctacgtgaa ggacggcatc
     1801 gtcacgtggg agatgcaggc actcgactac ccgctgctcg agtcgagcct gcccccgtac
     1861 gagccgcggg gctgccaacg cgggatctcc gcctcctggt acgtctacag cacgatccgc
     1921 gtgaagtacc cgtacatccg cggcgtcctc gccgacctct ggcgggaggc ccgcaagggg
     1981 cgcaaaccgg tcgaggcgtg ggcctcgatc cagaacgacg aggaagcgcg gacccgcatc
     2041 caggaagccc gcggcaaggg cgggttccgg cgtacgacct gggacgaagt gctcgagatc
     2101 gtcgcagcgg cctgcctcca caccgccgag aagtggggcc ccgatcgcgt gttcggcttc
     2161 tcgccgatcc cggcgatgtc gtacctctcg tacgcgggcg ggtcgcggtt cctccagctc
     2221 ctcggcggcg tgaacatgtc gttctacgac tggtacgcgg acctcccgaa cgcgttcccg
     2281 gagatgtggg gcgagcagac ggacgtctgc gaaagtgccg attggtacaa ctccaagttc
     2341 atcgtctcga tggggtccaa cctcaacatg acgaggaccc ccgacgtcca cttcatctcc
     2401 taggcacgcc acgagggcgc gaagttcgtc gtgctctcac cggacttcag tcaggtcgcg
     2461 aagtacgcag actggtggat cccggtcgag gccggccagg acacggccct ctggatgggc
     2521 gtcaaccacg tgatcctcac ggagttccac gcgaaccggc agatcccgta cttcaccgac
     2581 tacctcaagc ggtacacgga cagctccttc ctcgtcgagc tccagagcac gcccgaaggc
     2641 atcgtgcccg gccagttcct ccgcgccggg cggttggagc gctaccgcga cgtcgagaac
     2701 ggcgactgga agatgctcgt ctgggacgag aaggccggcg cggcgcgcat gccgaaggga
     2761 tcgatcgggt tccgctgggc gaaggaggag caggggaact ggaacctgaa gctcgaggac
     2821 ggcctcgacg acgagccgat cgacccgacg ctcagcttcc tcgacgctcc cggcgcggag
     2881 gtggcgcagg tcgcgttccg cgacttcagc acgcagaacg tcagccaacg cggcgttccg
     2941 gtgcgctgga tcacgaccga cgacggcggc cggacgctcg tcgccaccgt ctatgacctg
     3001 ctcttcgctc agcacggcgt gggacgggga ctcaccggcg actaccccga gagctacgac
     3061 gacctgcgtc cctacacgcc cgcctggcag gagcaataca cgggcatcgg acgcgacacc
     3121 gtgatccagc tcgcccgcga gttcgcatcc acggccgagg cgacgaacgg caagtcgatg
     3181 gtgatcatcg gcgcctccat caaccactgg tacaacaaca acctctccta ccgcgcaccg
     3241 atcaccgctc tcgtcctgtg cggctgctgc ggcgtcaacg gcggcggcat gaaccactac
     3301 gtcggccagg agaagctcac gctcttcgcc ccgtggacgt cgctcgcgtt cgccctcgac
     3361 tggcagaagc cgccgcgcca gcagcagtcg cccacctggc actacgtcaa ctccgaccag
     3421 tggcgctacg agggggactt caccgactac gcgccagtgc cgcccgacgc caaatgggct
     3481 aaagggcacg cgatggacct cgaggcgatg gccgtgcgca tgggctggat gccctactac
     3541 ccccagttcg accgcaaccc gatcgacgtc gccagggaag ccgaggcggc cggcgcctcg
     3601 acagccgaag aagtcgcgga atggacacgc gaacggatca ccgagggcga catcgggctc
     3661 gcggtcgacg acccggacgc ggccgagaac tggccgcgcg tctggctcat ctggcgaggc
     3721 aacgcgatct cgtcgagcgc caagggccat gagttcttcc tacgccacta cctcggcacg
     3781 cacgacaacg tcgtggccga agagcacgcg aaagacaagg tcaagagcgt cgtgttccga
     3841 gagcctgcgc cccgcgggaa gatggatctc gtcgtcgaca tcaacttccg gatggacacg
     3901 tcggcgctct actccgacat cgtcctgccc acggcgagct ggtacgagaa gaacgacctc
     3961 aacaccaccg acctccactc gttcgtccac ccgctacagg cggctgtgcc accggtgtgg
     4021 gagtcgaaga cggactggga catcttcaag gagttcgcgc gcaaggtgag cgagctcgcg
     4081 cccggcgtct tcggcgacgg gaaggtcaag gacgtcgtct ggtcaccgct cgcgcacgac
     4141 acgccggacg agctcgccca gcccgccgtt cgcgactggc gcgcggacga gtgcgaggcc
     4201 gagcccggcg tcacgatgcc gcacatccgc gtcgtcgagc gtgactacgc gaatctctac
     4261 aaccgcttca tctcgctcgg ccccggcgtg cgggagaacg gcctctcggc acacgggatc
     4321 aacatcccgg ttgcgtcggt gtacgacgag ctgctggcga accccgtcgg gggatcgccc
     4381 gacgtgcggc acatgcggtg cgtcgagtgg ggcggcgagc gctacccgag cctcgaggat
     4441 gcactcgacg ccgcgaacgc gatcctctct ctcgcaccgg agacgaacgg cgaggtctcg
     4501 ttcgccgcgt tcgaggccga ggagaagaag accggcctcg cgctcacgga catggcggat
     4561 gggaaccgcg gcgtccgcat gacgttcggc gacctcggcg cccagccgca ccgcaccctg
     4621 atcagcccct gctggacggg gatggtcaac gacggccgcg cctacgcggc ctggacgatg
     4681 aacgtcgagc ggctgatccc ctggcggacg ctcggcggcc gccagcacct ctacgtcgac
     4741 cacccctact acctcgactt cggcgagcac ctgccgacct acaagcccaa gctcgatccg
     4801 cggcagacgg gggacatcgt gcacagcccg acgggcgacg actcgctcgt cctcaactac
     4861 atcactccgc acgggaagtg gcacatccac tccacctacg gcgacacaca ccggatgatg
     4921 acgctctcgc gcggaatcga gccttgctgg atcaacgaca aggacgccgc gcgcatcgac
     4981 atccaggaca acgactgggt cgaggtcttc aacgacaacg gcgtgatggt cacgcgcgcg
     5041 gcggtctcgt cccgcgtcca ggcgggcacg tgcatgatct accacgcccc ggagcggacg
     5101 ctgtccattc ccaagtcgca ggtccgcggg aacagacgcg gcggcgcgca caactcattg
     5161 acccggacgc gcatcaaccc cgtccagctg gccggcggct acgcgcagtt cacctacgga
     5221 ttcaactact ggggcccgat cggggtcttc acccgcgaca cgtacgcggt cgtcaagaag
     5281 ctcgagagac tcgagtggtg acggcggcag ggagggagag acgcgacatg gatattcgcg
     5341 cacaggtgtc gatggtgttc cacctcgaca agtgcatcgg gtgccacacc tgctcgatcg
     5401 cctgcaagaa catctggacc gaccgcaagg gcggcgagta catgtggtgg aacaacgtgg
     5461 agacgaagcc cggcacgggc tatccgaccc tctgggagga ccaggagaag taccgcggcg
     5521 gctggaagca ggacggcgac ggcgtacgtc tgcgacttca gggcaaggcc tcgacgctcg
     5581 gcaacatctt cgccaatccg tcgctgccca ctgtcgacga ctactacgag ccgtggacct
     5641 acgactacga gaacctcttc gacgccccgg ccggcgacga ccagccgact gcccgcaccg
     5701 tgtcgatgat caccggcgag ccgatggacg tcgaagccgg ccccaattgg gacgacgatc
     5761 tcggcggctc gccgatctac gcggcgaatg atccaaatct cgccgaggtg ccgccagaag
     5821 agcgccgcca gctcgacgag ctcgagcaga tgatgttctt ctacctcccg cgcatctgca
     5881 atcactgcgt caacccgagc tgcgtggccg cctgcccggc cggcgccctc tacaagcgcg
     5941 gcgaggacgg catcgtcctg ctcagccagg agaagtgccg ggcctggcgc atgtgcgtgt
     6001 cgggctgccc gtacaagaag acgtactaca actgggccac cgggaaatca gagaagtgca
     6061 tcctctgcta cccgcgactc gagtcgggtc aggcgcccgc ttgcttccat tcctgcgtcg
     6121 gccgcatccg ctacctgggc gtgctgctct acgacgcctc gcagctggag cgagccgcct
     6181 cggttcccga cgaccagctc gtggaggcgc accgctcgct gatccaggat ccgttcgacg
     6241 aggacgtcat caaggcggcg aaggaaaacg gcgtcacggc cggcatgatc acggccgcgc
     6301 aagagtctcc cgtctaccgg ttcgtgaagg agtggaagct ggccctgcca ctgcacgccg
     6361 agttccggac cctgccgatg ctgttctacg tccccccgat gctcccggtg atcgcgaacg
     6421 tcgagaacgg ccggtacaag gtcgacgggt cgaacggcga cgtcgagtcg gctgttccgt
     6481 ccctctcgca catcgacaag tcacgcgcgc ccatccgcta catggcgagc ctgttctcgg
     6541 cggggaacga ggagatcgtc gccgaggtgt accgcaagct cttcgcggtt cgcgtcgtga
     6601 tgcgcgcgaa gaaggtcggc aacgtgcccg acgccgaggt cgacgcagcg ctcgaggcag
     6661 ggaagaccac ggacgaggag gcggaggaca tcttccggct gacctcgatg cccacgttca
     6721 aggagcgctt cgtcctgcca ccgcttgccc gtgagctcca gatggtggat ccgcacaaga
     6781 acaagcagga gaccggtttc ggcccccgcc aggccccgga gcggcgctgg tgagcacgat
     6841 agccctcaca cacgccgacc gtcaggttgt tgcgcgctcc ttcgccgacg tgctctcgta
     6901 cccgtatgcg tccccgatcg ccgtcgcggg cgccagcgcc gacctggtgc gcgagcgcag
     6961 cgacgaggcg gcgacgctgc tcgaggcgtt cgcgtcactg gtcgactctg caggcctcgg
     7021 gacgctccag gaggagtaca cgagggcgtt cgacctcgac acgatgtcga agaccgagcc
     7081 cacctgctac ccgtacgtgg ggcactacct cttcgacgag agccacaagc gcggcgcgtt
     7141 catcctcggt ctcaagaagc gataccgggc gaccggcttc gaggacgaca gcgacctccc
     7201 cgaccacctc gtagtgctcc tccgctttct caccgtgtgc gaggacgacg agctggccga
     7261 ggagctcgtc gacgacgcga tcctcccggc gctcgcacgg atggcggtcg cggccggtcg
     7321 gccgcccaca gaggaagtgt cgatccgcgg cgcctacatc ggcgtgctgc gggcgctcga
     7381 gctcacgctc agcgacggac gccccgagcg ggtggtcgac ccgctcgacc gcgaagccga
     7441 acgggaatgg ctgcgcgacc gtgacagcct cggcatcgac cgccagaggt gcggtcacta
     7501 ggaagtcagg aggcacgcca tgtggaacac gctgcttttc gccgtactcc cctacgtggc
     7561 ggtcgcgatc gcgattgccg gcggcatcgc acgctaccgg atggatcgct tctcgtactc
     7621 cagtcagtcg tcgcagttcc tcgaaagccg ggccctgttc tggggctccg tcttctggca
     7681 ctacggcatc ctggtcgtgc ttctcggcca tatcctcgcg ctcatgttct gggatccctg
     7741 ggccgcactc gtcagcgacc cggatcgcct ctacacgatc gaggtgatcg gtctcggcgt
     7801 gtcgatcctg gcgctcgtcg gtctcgccgt gctcgtcgtg cgacggctcg ctgttcgccg
     7861 agtcgcatcc gtcacgagcc cgatggacat cgcgctgctc gtcgtcctcg ccgcgcaggt
     7921 cgtcctgggc atctgggtgg ccctggccta tcgctgggga tccgattggg ccgtgttcac
     7981 cgccgtgccg tggatgcact ccctcttcaa gctcaacccg aagccggagt tcatggctgc
     8041 gcttcctgtc gtcgtcaagg tgcacgcact cggcggcttc ctgctcatcg ccctgttccc
     8101 gttcacccgg ctcgtccaca tcgtcaccta tccggtgacg tacctctggc gctcctacca
     8161 ggtcgtcgtg tggaaccgaa gtcggcaaca ctgagcactg cacgcgccgg cacgctgagg
     8221 cccgaacggc ggcggatccc agtcaccgtg ctcgcgggct tcctgggttc cgggaagacc
     8281 actctcctga accgcatcct cgccggcggg cacgggatga aggtcgcggt gctcgtcaac
     8341 gacttcggct cgatcaacgt cgacagccgg ctcatcgtga gccgcgacgc gacgatgatc
     8401 accctcgaca acggctgtat ctgctgctcc atcagcggtg acctcgtctc ccagctgacc
     8461 gacctcctgg aagggccgtg gagtcccgag cacgtgctcg tggagacgag cggcgtctcc
     8521 gacccgggcc ggctcttgct ggcgttccgc catccccacc tgcgctcact ggcacgggtc
     8581 gacgggatga tcaccatggt cgacgcggca ggcttcgaga cgattccgcc cacgatgcac
     8641 gagctcgccc ggcgccagct cgcgggcgcc gacctgatcg tcttcaacaa ggtcgacctc
     8701 gtcgccgaag acgcgctcgc ggcactccgc ggacgtttga catatccgca ggcgcggacg
     8761 atcgatgcga gccacgggaa cgtcccgctc gaactcgttc tcgggataga ggcgccggag
     8821 cgggctccgt cggagaacgg aggcgcctcg tctccggagc acggcgaggc gttcgcgacc
     8881 tggtcttggc gctcgtcccg ccccctggcc ctcgctgagg tgcgcgaagt cctgggctcg
     8941 cttccgagca acgtctaccg ggcgaagggg ttcctctacg tggccgaggc cccagccgag
     9001 cgaatcgtcg cgcacgtcgt cggccgcagg gtcgacctgc ggccctccgg ctggtggggc
     9061 gacgccgagc cgcagaccga gctggtgttc atcagcctcg agccccggat cgacgccgtc
     9121 ggcctgcgcc gacgcctcag agcgaccacg gcaccgtcgc aggcctgaca gccccgcgcg
     9181 ggagagccgg atgcatcccc gccagggttg tcgtgaccgc ttctcgccaa ctcgcggcga
     9241 tcgcacagag ctggcacaaa ttgatcagct cgtcgcggcg cgcgccccat agtctgcaga
     9301 ggtgccgaca ggcccatccg gctcgaagag agcagcccgc ccaccgctgg tcgttgcctg
     9361 ggaggtgacg agcgcctgcc cgctcgcctg ccgccactgc cgcgcaaccg cacaggtcgc
     9421 cccccatccg gctgagctga cacacgagga ggggcgcgcg ttcatcgccg atctggcgaa
     9481 ggacttcccc ggcgccgtcc tgatcctgac gggcggcgaa ccgctcacgc gaccggacac
     9541 gctggacctc gcccgcgaag ccggagccct gggtctccag gtcgccctct cggtggacgt
     9601 cggctggctg ctcacgcccg agcgctgcac tgcaatccgc gaggcgggcg ttcgtcgggt
     9661 ctctttcagc atccacttcc ccgacgcgga gcagagcgac caattcgccg atacgcccgg
     9721 gttcttcatg gcggcgcggt cgggcctgga gaatctcgtc gcggccggcg tccccttcca
     9781 gctgcacacg agcgtgatgc gggcaaacgc ggagctgctg ccgcgcatct acgacctcgc
     9841 tctcgagatc ggcgctgcgg gctgggagct gttcgtcctc gtcccgacgg gacgcggctc
     9901 cgggttggcc ggcgaggagc tcgagccgct cgagcaggac cgtgtcctgc gttggctgta
     9961 cgacctccag cggacggcgc cgttccccgt caagcagatc tgcgcgccac atttccgccg
    10021 cgtcgaggcc caggttgccc gtgagcgcgg cgagcgccgc cctgagcgcg tcacgacgcg
    10081 actgagcgtc ctgtcgcgcg gctgcctgtc ggggaacggc ttctgcttcg tctcgcacgt
    10141 cggcgacgtc tgcggctg
//
LOCUS       DBNGNK010000118         4631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1237841,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000118 DBNGNK010000000
VERSION     DBNGNK010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1237841"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="AC150V_03370"
     CDS             <1..380
                     /locus_tag="AC150V_03370"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3761990.1"
                     /translation="WEHMKDRQWGRIVNMASVAGTLGGFGQASYSTTKAGLLGLTKTL
                     AMEGGRHGITCNAVVPGIIGTEAFHMANEAMNERIANRTVFKRPGRPEDIAGAIAFLC
                     SDLAVYITGIELNVSGGVELFVF"
     gene            430..645
                     /locus_tag="AC150V_03375"
     CDS             430..645
                     /locus_tag="AC150V_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761991.1"
                     /translation="MRRLLLLAAVSLVVLLPTVIAQASPSGSGQPAPPVPAAVAGGGK
                     AAPAGPHAQGGPHGKKGPCPFAADPAI"
     gene            665..1843
                     /locus_tag="AC150V_03380"
     CDS             665..1843
                     /locus_tag="AC150V_03380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3761992.1"
                     /translation="MSAQATGEGISFTPTPEQRELKELAHEFAEKELRPAAAESDRTG
                     EYPTGLLAKAARLGLTSHAIPAEYGGGGVAAVTSVLVAEELSWGCAGLAATIGATMFP
                     VRPLLVAGSEVQRERWLPRLASATGCLAAIAFTEPAAGSDVAAIATRARRDGDGYVLN
                     GEKCYITNGGAAEITVLFAKTDEGPTAFVVETGDPGVLPGRREDTVGLRASRTASIVL
                     DNARLAADRRLGEEGTGFVLAMDFFEHSRPQVAAAAVGLARAAFEYATEYARGRETFG
                     RPIVQRQGISFKLAEMGMAIEAARLLTWRAAAALDAGADAGLLGSYAKAFAADTAMRV
                     TTDAVQVLGGAGVNRDHPVEKWMRDAKVLQIVEGTSEIQRQIAATYLQEGWERTGRPR
                     "
     gene            1928..3835
                     /locus_tag="AC150V_03385"
     CDS             1928..3835
                     /locus_tag="AC150V_03385"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR3761993.1"
                     /translation="MRAESSTYRLAARRSRARRLALQRAAVLAALVVVLAVVFGLAYA
                     GSSSTLAEGTRVAGIDVGGLSAAEAVERLAARGKALARTPVTFAVGGARYRIEPVQLG
                     VRADWAGAVAAARAENDGFGPLRGFKRLHTRFFGEEVAPGVAVSNAALEYKLGQIAAA
                     VDRPMVEPSLRMKGFGVVVAPGRSGTRLDREVAADVIVRSLASFERGKTVTLPVEKLV
                     PTVSRADLQPALASARAALSRRPLKLVLGTSSWTLSRKEIATLLALPHGGATTVGVGG
                     AGAEAYLDRLAGAVNREPQNATFAVKPGGIGIVPHQPGLTLDLPATARAIQRALLAPA
                     GGRTAKLVAHATPPERTTKAAQAMGITGVVASYTTSYGGTPGRLHNVQLVARLIDDTL
                     IAPGEVFSFNATTGERNSDRGFQEAPVIINGELQNGIGGGVCQVSTTVFNAAFDAGLS
                     IESRTNHALYISHYPLGRDATVNYPDLDLRFKNDTGRWLLLRAFVNPGSLTVNLYGTP
                     TNRRVETETTPLAVTSEAPVEEVEDATLLEGKRVVEEPGAPERATSVRRKVYDANGNL
                     LYDSTWHSTYVAEPEVVRVGTKEKPKPKPVTLPGLLPGGPVAIVPGPSGVTPAEEPAA
                     SLPPSEPGSLP"
     gene            complement(3798..4232)
                     /locus_tag="AC150V_03390"
     CDS             complement(3798..4232)
                     /locus_tag="AC150V_03390"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3761994.1"
                     /translation="MPAGFTNSHDIVVRFAETDAQGIAHHASFVVWLEVARVAYLGEH
                     AGGYNAIRDQGIEALTTAVHVDYHSAAYFDDRLIVWLRCTDVRGARFRYEYRVERDGD
                     LIAEAHTEHATVDRATHRPTRVPEWLVHAMTTAESPARTAAG"
     gene            4256..>4631
                     /locus_tag="AC150V_03395"
     CDS             4256..>4631
                     /locus_tag="AC150V_03395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3761995.1"
                     /translation="MSAAALAELIRQRQQCVVLTGAGMSTESGIPDFRSPTGLWATVD
                     PFEVASIDAFRRDPGRTWQFYGPRIRALLDAQPNAGHLALAVLEQAGLVRAVVTQNID
                     TLHRRAGSCDVIEVHGSISTATC"
BASE COUNT          711 a         1564 c         1654 g          702 t
ORIGIN      
        1 tctgggagca catgaaggac cggcagtggg gtcggatcgt caacatggcc tccgtcgccg
       61 gcacgctggg gggcttcggg caggcgagct actcgacgac gaaagcggga ctcctgggac
      121 tcacgaagac gctggcaatg gaaggagggc gacacggcat cacgtgcaac gccgtcgtgc
      181 cgggcatcat cggcaccgag gcgtttcaca tggccaacga ggcgatgaac gagcgcatcg
      241 cgaacagaac cgttttcaag cgcccgggcc ggccggagga catcgccggc gcgatcgcct
      301 tcctctgctc ggatctcgcc gtatacatca ccggcatcga gctgaacgtg tccggcggcg
      361 tcgagctctt cgtcttctag agggcgggag cgtttgtgag tgtctgtccg agccagtacg
      421 atgccgggcg tgaggcgcct cctcctgctc gccgcggtct ccctcgtcgt gctgctgccg
      481 acggtgatcg cgcaggcgtc gccgtcgggc tccggtcagc cggcacctcc ggtccccgcg
      541 gccgtcgccg gcggcgggaa ggcggctccc gccgggccgc acgcgcaggg cggcccgcac
      601 ggcaagaagg gaccatgtcc cttcgcggcc gacccggcga tctgagcgaa ctcgagcccc
      661 ggcgatgagc gcccaggcga cgggcgaggg catctcgttc acgcctacgc ccgagcagcg
      721 ggagctcaag gagctcgcgc acgagttcgc cgagaaggag ctccgccccg ccgcagcaga
      781 gtccgaccgg accggtgagt atccgacggg gctgctggcc aaggccgccc ggctcggcct
      841 cacatcgcac gccattcccg ccgagtacgg gggcggcggc gtcgccgccg tgacgtccgt
      901 gctcgtcgcg gaagagcttt cctggggctg cgccggactc gcggccacga tcggggccac
      961 catgttcccg gtccggccgc tgctcgtcgc cggcagcgag gtgcaacggg agcgctggct
     1021 gcctcggctc gcctcggcga cgggttgtct ggcggcgata gccttcacgg agccggcggc
     1081 cgggtcggac gtcgccgcga ttgcgacgag agcccgccgc gacggagacg ggtacgtcct
     1141 gaacggcgag aagtgctaca tcacgaacgg cggcgctgcc gagatcaccg tcctcttcgc
     1201 caagacggac gagggcccga ccgccttcgt tgtggagaca ggagatccag gcgtgctgcc
     1261 ggggcgacgc gaggacaccg tcggcctgcg cgcgtcgcgt accgcgtcga tcgttctcga
     1321 caacgcccgg ctggccgctg accgtcggct cggagaggag ggaaccggct tcgtcctcgc
     1381 gatggacttc ttcgagcact ctcgacctca ggttgcggcc gcggccgtcg ggctcgcgcg
     1441 cgctgcgttc gagtacgcga cggagtacgc acggggaaga gagacgttcg gccgaccgat
     1501 cgtccagaga caggggatct ccttcaagct cgccgagatg gggatggcga tcgaggccgc
     1561 caggctgctg acatggcgcg cggctgcggc gctcgacgcg ggtgccgacg ccggactgct
     1621 cggctcctat gcgaaggcct tcgccgcgga cacggcgatg cgggtcacga cggacgcggt
     1681 gcaggtgctc ggcggtgcgg gcgtcaaccg cgaccaccct gtcgagaagt ggatgcgcga
     1741 cgcgaaagtg cttcagatcg tcgaagggac gtcggagata cagcggcaga tcgcagccac
     1801 ctatctgcag gagggttggg agcggaccgg gcgaccgcgc tgacgccgac ccgccgggcg
     1861 gaacctctcg caaagcgcag tttgaagcgg ttgagcgggc ggcaaggtcg tatagagttc
     1921 gtcgctcgtg cgcgcggaat cgtcgaccta tcgtctggca gcgcgccgca gccgggctcg
     1981 ccggctggcg ctgcagcgtg ccgccgtact ggcggccttg gtcgtcgtgc tcgccgtcgt
     2041 cttcggtctc gcctacgcgg gttcctcctc caccttggcc gagggaacga gggtcgccgg
     2101 tatcgacgtc ggcgggttga gcgccgcgga ggctgtcgag cggctcgcgg cgcgcggaaa
     2161 ggccctcgcc cgcacgccgg tgaccttcgc cgtcggcggc gcccgatacc ggatcgagcc
     2221 ggtgcaactg ggcgtgaggg ccgactgggc gggcgcggtc gctgcggccc gcgccgagaa
     2281 cgatggcttc gggccgcttc gcggcttcaa acgactgcac acgcgcttct tcggcgagga
     2341 ggtcgccccg ggggtcgccg tctccaacgc ggcgctcgag tacaagctgg ggcagatcgc
     2401 ggcagccgtc gacaggccga tggtcgagcc gtctctccgc atgaaggggt tcggagtcgt
     2461 cgtcgcgccg ggccggtcgg gcacgaggct cgaccgtgag gtggcggccg acgtcatcgt
     2521 gcggtcgctc gcctcgttcg agcgtggcaa gaccgtgacc ctccccgtcg agaaactcgt
     2581 gccgacggtg tcccgcgcgg atctccagcc tgctcttgcc tctgctcgtg cagcgctctc
     2641 acggcggcct ctgaagctcg ttctgggaac gtcgagctgg acgctatcga ggaaagagat
     2701 cgccacgctg ctggcgctgc cccatggcgg agccacgacg gtcggggtgg gaggtgccgg
     2761 ggcggaggcc tatctcgacc ggcttgccgg cgcggtgaac cgagagcctc agaacgcgac
     2821 gttcgcggtc aagcccggcg gcatcgggat cgttccgcac caacctggtc tgacgcttga
     2881 tctgccggcc acggcccgcg cgatccagcg agctctgctg gctccggcgg gcggtcggac
     2941 cgcgaagctc gttgctcacg ccacaccacc ggagcgcacg accaaagccg cccaagcgat
     3001 gggcataacc ggcgtggtcg cgagctacac gacgtcctac ggcggcaccc cagggcggct
     3061 gcacaacgtc cagctcgtcg cgcgtctcat cgacgacacg ctcatcgctc ccggggaggt
     3121 gttctcgttc aacgcgacca cgggagagcg caactctgac cgcggcttcc aggaggctcc
     3181 cgtgatcatc aacggggagc tccagaacgg catcggcggc ggcgtctgcc aggtctcgac
     3241 caccgtcttc aacgcggctt tcgacgccgg cctctcgatc gagtcgagga cgaatcacgc
     3301 tctctacatc agccactacc cgctcgggcg ggacgcgacc gtcaactatc cggatctcga
     3361 tctcaggttc aagaacgaca ccggccggtg gttgctgttg cgagcattcg tcaacccggg
     3421 ctcgctgacg gtcaacctct atgggacgcc cacgaatcgt cgggtcgaga ccgagaccac
     3481 gccgctcgcg gtgacgagcg aggcgccggt cgaggaggtc gaggacgcga cccttctaga
     3541 ggggaagcgg gtcgtggaag agccgggtgc gcccgagcgc gcgacgagcg tgcggcgcaa
     3601 ggtctacgac gccaacggca acctgctgta cgactcgacc tggcattcca cctacgtcgc
     3661 cgagcccgag gtcgtacgcg tcgggacgaa ggagaagccc aagccgaagc cggtgacgct
     3721 gccgggtctg ctgcccggag gtccggtcgc catcgtgccg ggcccgtccg gcgtgacgcc
     3781 tgccgaagag ccggccgcta gcctgccgcc gtccgagccg ggctctctgc cgtagtcatg
     3841 gcgtggacga gccactccgg tacgcgggtc ggccggtggg tggctcggtc gaccgtcgca
     3901 tgctcggtgt gcgcctcggc gatcaggtcg ccgtcccgct cgacgcggta ctcgtagcgg
     3961 aagcgggcac cgcggacgtc ggtgcagcgc agccagacga tgagccgatc gtcgaagtac
     4021 gcagcactgt ggtagtcgac gtggactgcg gtcgtcagag cctcgatgcc ctggtcccgg
     4081 attgcgttgt atccgcccgc atgctcgccg aggtacgcga cgcgagccac ctcgagccag
     4141 acgacgaaag aggcgtggtg cgcaatgccc tgggcgtcgg tctctgcgaa gcggacgacg
     4201 atgtcgtgtg agttcgtgaa cccggccggc accaaacgca tcctagaatc gacttgtgtc
     4261 ggccgctgca cttgccgagc tgatccgtca gcggcagcaa tgcgtcgtcc tcaccggggc
     4321 ggggatgagc accgagagcg ggatccccga cttccgttcg ccgacgggcc tctgggccac
     4381 cgtcgatcct ttcgaagtgg cgtcgatcga cgcttttcgg cgagatccgg ggcgcacctg
     4441 gcagttctac gggccgcgta tccgcgctct cctcgacgcg cagccgaacg cagggcacct
     4501 tgcgctggct gtgctcgagc aggccggact cgtccgcgcc gtcgtcacgc agaacatcga
     4561 caccctccat agaagggcgg gcagctgcga cgtgatcgag gtgcacggct cgatcagcac
     4621 ggcgacctgc c
//
LOCUS       DBNGNK010000119        16959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1524871,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000119 DBNGNK010000000
VERSION     DBNGNK010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 16959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16959
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1524871"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..1061
                     /locus_tag="AC150V_03400"
     CDS             210..1061
                     /locus_tag="AC150V_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3761996.1"
                     /translation="MNTTQNGGAAGSRRRAVLLLAGLLAAGAALLVTLGLGSSSGTAA
                     QSQYAPGNTKAPTISGKAAQNEVLTVSEGTWTGTQPIVFSYQWLRCNESGGNCVNIPN
                     ATSKTYTVVAADVNRTIRASVKASNNQGSASTLTLQTAKVTGPLGPAGAIKLPNGETS
                     IPVTSVPANERLIVDQVTFSPRPIRSRTTNITVRVKVKDTRGYVVRDVSVFVRTTPLV
                     SRIQNNRSRTGQDGWVTYTDVPRPNFPAIRNGTNLQYFVKAYRNGDPPLAGVAGYRLV
                     QTPLRKP"
     gene            complement(1085..2395)
                     /locus_tag="AC150V_03405"
     CDS             complement(1085..2395)
                     /locus_tag="AC150V_03405"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3761997.1"
                     /translation="MAALGALAGLLAWQLLSLVWTRAVPLTALEPQRTLLYLAALAAA
                     LMWLRRELVRATLLGVLAGSLAICLWNLAFRWGAGETTGEEATPIGYGNGLGLLAAVA
                     ILLGVGLALETSGPMRWALVAGCSPAVAVLWLSESRGAWIALGAGAAAGLLSRRNRPT
                     LGLARAAAGGIALSLAAGGAASDERRAYWHASLAEWVDSPVLGSGGGTWGRVWLERRA
                     ELFPAVDAHSLYIEALSELGPVALALLAVSCAAPLVAAGRAAASPGVAAAAAAWVALL
                     VHFAADWDWELPGVTVAGLVCAAALLVSARSDVIGVPRGVSIAASLTVIALTVPLLAG
                     NVLLSRSEEALRDGDAQAAVREARRAARLQPWAAEPWRIRAEAELALGDRAAAARSSA
                     KSIERDADDVEVWRTAARVRSGAGRQAAEARVLELDPLAAAPSR"
     gene            complement(2556..3068)
                     /locus_tag="AC150V_03410"
     CDS             complement(2556..3068)
                     /locus_tag="AC150V_03410"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit B family protein"
                     /protein_id="HIR3761998.1"
                     /translation="MDVLVLIDKLDDLVHNAKAVPLTDQVRIDREEIYDILDQMRATI
                     PEEIKQARWIVKERQEMLAEAKRECDRLIGEAREQAVREASQTEIVKLAENQAQEIVD
                     DARRQARETRLELEDWADGILSTLEVNLDKFLGAVRRGRERLHERSQETVVAGIGPQD
                     LQQPDEESYL"
     gene            complement(3082..3597)
                     /gene="coaD"
                     /locus_tag="AC150V_03415"
     CDS             complement(3082..3597)
                     /gene="coaD"
                     /locus_tag="AC150V_03415"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR3761999.1"
                     /translation="MITAICPGSYDPVTNGHLDVIGRAAAIFDRVVIGVVRDPSHKSA
                     TFSIDERVAFLEDALADFENVKVDVFSELVVDFARRWGAKTMVKGLRVIADFEWEFQM
                     NHLNRLLAPEIETMYVMASPAYSFVSSSGVKEIASFGGKVDDLVPEAVASRFRELFPD
                     GRPGAPVSPQE"
     gene            complement(3658..4245)
                     /gene="rsmD"
                     /locus_tag="AC150V_03420"
     CDS             complement(3658..4245)
                     /gene="rsmD"
                     /locus_tag="AC150V_03420"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR3762000.1"
                     /translation="MRIIAGSRKGARIAAPSGKATRPTGDRVREAAYNLIGPVDGATV
                     LDLFAGSGAMGLEALSRGAARCVFAEADRVACKVISANLEKLRLTGAVVHCRDALSVL
                     REERSASRRYDLVLCDAPYEEYTRLEPALEQLLPPVLSPAGLLVLETAARTEPSLGLE
                     EVTSRRYGSARLTLYRPRVRAQSEHDGPKPPSGDK"
     gene            complement(4296..6398)
                     /gene="recG"
                     /locus_tag="AC150V_03425"
     CDS             complement(4296..6398)
                     /gene="recG"
                     /locus_tag="AC150V_03425"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR3762001.1"
                     /translation="MATTTRTRLPAGFAGAEPPDTWPRPRSAPRPEALSPPVTTLPRV
                     GAAVAAKLRRLGIETVGDVLAHRPRRYEQPVPERAIAELFGDEEAVIEGEVLDVSGRR
                     HGRLHILTAKVSDETGVVRATWFNQPWLERQLSPGTRVRLRGRNGRRGFVVESHDLGE
                     AAETADFAPVYPASEELSQKQLRGIAEAALVHAGAAGDRLPVGLRLAEGLPLRQDALV
                     ALHRPLSLEEAEVGRRRLAFDELLVLQLALARRAAAHEGAMAVALPPPGELAARYRSV
                     LPFTLTDAQEGATAEIDADLARTVPMQRLLQGDVGSGKTAVALYALLRAVEAGRQGAL
                     MAPTETLAEQHFLTIEDLCRALEVRVALLTSALPAKEHAHVRGLIEAGDVDIAVGTHA
                     LIQREVEFADLAVAVVDEQHRFGVEQRAALVEGRSPHVLHMTATPIPRTLALTVYGDL
                     SVSEIGALPADRKPVITAWVAAERSSEAYQRLRRHLDAGRQAYVVCPLIDESETSVAR
                     AAEAEAERLRGAELSGYRVGCLHGRLRTGDRRELMRQFKAGELDVLVATTVIEVGVDV
                     PNATIMIVQEADRFGLAQLHQLRGRVGRGAEQSYCLLVSAPKEMLTDNARERLEAMVS
                     SADGFELAEKDLEIRGEGQLLGGRQSGLSDLHFARLRQDGGLLRRARRVARELESEGL
                     LGDEVDRLLGAAEHLGEN"
     gene            complement(6443..7312)
                     /locus_tag="AC150V_03430"
     CDS             complement(6443..7312)
                     /locus_tag="AC150V_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR3762002.1"
                     /translation="MTLTRENTAIVLDSTSDFPDAANRFGNLREVPLYVRFGDDSLRD
                     HVDIDPTTFYERLAVASELPTTSQPTPQDFLSVYQELVEAGFERIYSLHLSAKLSGTH
                     QSACVAADELGGDVVRVVDSETASLAVALLALAVQHRLGRGSSDEEIESLVDRFKQVN
                     GVVFTVGTLEYLQKGGRIGKGQAFAGALLNVKPILGVADGEVAAVARVRGRQKALKEF
                     ARAFEAGTHEGPGIRVAIAHANAPDWVDVLTDMTHKVRADAEIALVETLGAVVGTHAG
                     PGAVGYFWFQDEV"
     gene            complement(7309..7698)
                     /locus_tag="AC150V_03435"
     CDS             complement(7309..7698)
                     /locus_tag="AC150V_03435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3762003.1"
                     /translation="MSEQAAIRVLLVEDNDVYRSSLELLLGMQPGLEIVGSIADGRSA
                     ADAAVNLEVDVALMDFRLPGLDGAEATAAVLASRPAAAVLCLTAEATDDERESVRAAG
                     AVGFVEKGEPIERLVAAIKHAAREGAQ"
     gene            complement(7695..9284)
                     /locus_tag="AC150V_03440"
     CDS             complement(7695..9284)
                     /locus_tag="AC150V_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR3762004.1"
                     /translation="MNVARANELAAAALANLEAHRQHIDDLNVYPVPDGDTGTNLTLT
                     VRAVVEALGSSTAEGPEPVAKELSRAALMGARGNSGVIFSQILRGFAEVLGRHDDVDG
                     EVLAKAFRASSDAAYRAVRRPVEGTMLTVVREMAEEAELPDVRALGKEEALWRAVERG
                     EQALARTPEMLPVLAKAGVVDAGGAGLVEIVRGIALGVSGEPIPDAPVDAEELGVDAI
                     HQELSRFRYCTVFVVEGEGLDLDALEQQLEQLGDSLLVVGDATALKVHVHTDDPGAAL
                     SVGTAVGTIDGIEIANMHEQTQEREERLSDELADIPTLVTALVAVAPGDGNRRLFESL
                     GATRVIEGGQTMNPSTAEILDAVEAAPADDVIVLPNNKNVLLSAEHAAEHSDKTVLVI
                     PTTSVQAGIEAMVSFNRGLAAAENEEAMLEAVAEVVTGEVTRASRDTEIDGVEVRAGA
                     WLGLVDDRVVTCGEEFDAVVDAVVERLVADGRETLTILVGEDAPDVDRLRARIEQRHP
                     HVTVDPRDGGQPHYPLLLAAV"
     gene            complement(9281..9634)
                     /locus_tag="AC150V_03445"
     CDS             complement(9281..9634)
                     /locus_tag="AC150V_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR3762005.1"
                     /translation="MDKGIHIVASSELGRVGVSTEAIQQIVGHVAAEAYGVVEMAGRR
                     LRLRPRDPLTSGIDVRGDRDGLELVLHVVVEYGLNLAEIASTLRSRVAYEVERQTGLK
                     VASVDVRIDDVRRSS"
     gene            complement(9705..9896)
                     /gene="rpmB"
                     /locus_tag="AC150V_03450"
     CDS             complement(9705..9896)
                     /gene="rpmB"
                     /locus_tag="AC150V_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR3762006.1"
                     /translation="MSKVCAICGKKPSFGNNRSHSMVATRRRFNPNLQRVRVLLAGKP
                     KRAYVCTRCLKAGKVEKAV"
     gene            complement(9919..10902)
                     /gene="ribH"
                     /locus_tag="AC150V_03455"
     CDS             complement(9919..10902)
                     /gene="ribH"
                     /locus_tag="AC150V_03455"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:TIGR00114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR3762007.1"
                     /translation="MSDRHETDHKRAPTGPYDHARWEDSDEDARPASEEEPEGKDDID
                     LARENVLEGWPIRPDEEDGGPELDGGLGAGPEPESEPERASESSTDAAPVVEEAVAAG
                     DAEPVEAGLAEEISLDERELTVAAAAVAASAPPAETPSAPAPEAEPDDVPGAEAEVPA
                     SEHAPGTFAVPPGYAVLEGQPNGARRAVGVVVSRFNGDITTRLLQSALAELEERGVTG
                     EAITVMPVPGAFELPLAAMALAKTRRFSCVVALGCVVRGDTPHFDYVAGEAASGLQLA
                     GLETGVPVSFGVLTLEDADQAETRVDKGAEAVRTALEMADVFANLRTAAAQ"
     gene            complement(10899..12188)
                     /locus_tag="AC150V_03460"
     CDS             complement(10899..12188)
                     /locus_tag="AC150V_03460"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR3762008.1"
                     /translation="MSSEPALVGEETPFSSIEEAIEDVRAGRFVVVVDDEDRENEGDL
                     TIAAEFTTPEAVNFMATHGRGLICLCLTGERCAELGLRQMTEQNETPLGTAFTVSIEA
                     REGVSTGISAHDRARTIQVAIAPEATARDVVSPGHVFPLQARPGGVLQRAGQTEAAVD
                     LARLAGLTPAGVVCEIMKDDGTMARVPDLVEYCGRHGLKMITVADLIEYRRRTEKLVE
                     RVVSVRLPTQYGDFTAVAFRELLTGKQHVALVKGDVEGKDGVLVRVHSECLTGDVFHS
                     LRCDCGEQLELALARIEQEGRGVLLYLAQEGRGIGLLNKLRAYELQENGRDTVEANIE
                     LGLPADAREYGIGSQILAELGLTSIRILTNNPKKIAGVAGYGLTVVSQEPIEVQPNEQ
                     NRRYLAVKRDKLGHTIGRKLHHQRLRFDPDLFEGAEL"
     gene            complement(12209..12808)
                     /locus_tag="AC150V_03465"
     CDS             complement(12209..12808)
                     /locus_tag="AC150V_03465"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR3762009.1"
                     /translation="MFTGIVRELGRIARIEGSDADGARIFVETPRIALEIGIGDSVSV
                     NGCCLTAEVADGGLIAFHAVPETLRRTTLGDLAEGDEVNVEPALRVGDPLGGHYVQGH
                     VDGVGRVQSVEAEGEGLRVFVEASSDILRYCVEKGSITVDGVSLTVTDVADDAFGVAL
                     IPHTLEGTTLSRLAPGRRVNLENDVLAKYTARLLSMPEA"
     gene            complement(12818..13333)
                     /locus_tag="AC150V_03470"
     CDS             complement(12818..13333)
                     /locus_tag="AC150V_03470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HIR3762010.1"
                     /translation="MTYKAAVTVDGRVTVPGSRWVTGEASRRVVHELRARSDAVAVGM
                     GTVRADDPRLDARAVDAVRQPRRLAFGAGPLPEDSTLELRSGPLDDELRALGDEGVQS
                     LLLEGGPTLATAFLDAGFVDKLLLFVAPTLSGDGPHLLGPLRGPIQLSHLTSVHVGDD
                     VMLEAYLHEPR"
     gene            complement(13346..13756)
                     /gene="ribD"
                     /locus_tag="AC150V_03475"
     CDS             complement(13346..13756)
                     /gene="ribD"
                     /locus_tag="AC150V_03475"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR3762011.1"
                     /translation="MSVARALELAERGRGTTHPNPVVGAVLVRDGVVVGDGWHERAGG
                     AHAEIVALEAAGERARDATLYVSLEPCSHHGRTPPCVDALIAAGVASVVIGALDPNRD
                     VDGVERLRAAGVDVRVDDSFEARRQNEAWRTWIA"
     gene            complement(13753..14433)
                     /locus_tag="AC150V_03480"
     CDS             complement(13753..14433)
                     /locus_tag="AC150V_03480"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR3762012.1"
                     /translation="MSWADWIRDDVEIEPSLYAADFSRLGEQIRSLLAAGVRIFHFDV
                     GDGHFIPPVTIGPVVLRSIAPLVHAAGAVLDCHLMVADPAHHFAEFAQSGADSVTFHV
                     EATDDPAGVAAQAREHGLAVGAVFNPGTEPEVAAAFARSAAAEMILVMSVWPGYSGQA
                     FIPGSVGRVARLRELVDLPIQVDGGVDETNAGALRSAGASALVAGSAIFADPDLVAAY
                     RRIAAAAR"
     gene            14504..14947
                     /locus_tag="AC150V_03485"
     CDS             14504..14947
                     /locus_tag="AC150V_03485"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3762013.1"
                     /translation="MGVHVAVETRIDRRQDDVRAYVIDPANDPEWIGGITEARLLTEG
                     DVRLGSRVARVASFMGRRIEYVNEVTVLTDDELTMRSVAGPFPMEITYRFDREGEGTR
                     VGVRVRGETGRFYRIAEPLLARRVRSSITEDVERLRSILESAPVS"
     gene            complement(14940..16187)
                     /locus_tag="AC150V_03490"
     CDS             complement(14940..16187)
                     /locus_tag="AC150V_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR3762014.1"
                     /translation="MASRPSVIAPARRAAYDVLIRVFEDAAYTDRALRGAVDGLEERD
                     RRLAQQLSYGAVQRVRTLDHAIERLGRRPVPKLDPPVRAALRLGAYQLGFLDGVPRYA
                     AVNESVELVRRAGLERAVPFTNAVLRRIADGIADLIRGLPDRTPVEAALRHSYPDWVA
                     EVWWRDLGAADAVRLMQAQNEPPETVVRLCRGSVDGTPDTEIPGAWHVERVDEAALAE
                     GRIWPQSRGSQLAGLAVGARDGERTLDLCAAPGGKATMLAGEVLAVEVHEGRARQLQE
                     TARRLGAANVSVLVADARALPPDLGEFDHALVDAPCSGLGVLAARPDLRWRGKPLPEL
                     QLELLRAAGASVRHGGTVTYSVCTVNADESEAVVDAARLEVDPSLADEWPRFRHPRRP
                     DFLQTLPHVHGTSGFFVARLRVS"
     gene            complement(16166..>16959)
                     /locus_tag="AC150V_03495"
     CDS             complement(16166..>16959)
                     /locus_tag="AC150V_03495"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR3762015.1"
                     /translation="AVAATAAFGADVLTRLAARHEVTAVLTRPDAPRGRGRKLGAPPA
                     KEAAESLGLRVLQLAKPKAGLELDARIVVVCAYGLIIPEGLLSERLWLNVHPSLLPRW
                     RGAAPVERSIMAGDLETGVTIHETVKNLDAGPVAAQRSFPIGPDDDAGDVYARAAAVA
                     SELLDGVLADPSPSFVAQSDVGVTYADKIGPGDRSLDLSRPANELVRQVRALSPHIGA
                     RAELRGRPVTVWRARVAEDGSFAPLEVQPDGKRRMGYEEWLRGLR"
BASE COUNT         2589 a         6126 c         5752 g         2492 t
ORIGIN      
        1 gtaggcaggt gcggcggcgc cgccgtccga cggcgccgcc gctctccatc cgaccgcctc
       61 cgcacactgc cacagggcct cagcacggcc gcaggacccg ttaggggtct gtaagagccg
      121 cgagtaaggg ctttgtaagc cgcgtcgaga cgtgggcaga gtgaggacca cagctcgttc
      181 ggacgagcac gagaaaggag aaagtgctga tgaacaccac acagaacggg ggggctgccg
      241 ggagccgccg ccgcgccgta cttctcctgg cgggactgct cgccgccggc gccgcgcttc
      301 tggtcacgct cgggcttggc agctcgagcg gcacggcagc gcagtcccag tacgcgcccg
      361 gaaacacgaa ggctccgacc atttcgggca aggcagctca gaacgaggtg ctcaccgtca
      421 gcgaagggac gtggacgggc acgcagccga tcgtgttctc ctaccagtgg ctccgctgca
      481 acgagtccgg cgggaactgc gtcaacatcc ccaacgcgac gagcaagacc tatacggtcg
      541 tggccgccga cgtgaacagg acgattcgag ctagcgtcaa ggcgagcaac aaccaggggt
      601 cggcctcgac cctgacctta cagacggcaa aggtgaccgg gccgctcgga ccggcgggag
      661 cgatcaagct gccgaacggt gagacgtcga tcccggtgac gagtgtcccg gcgaacgagc
      721 gcctcatcgt cgaccaggtc accttctcac cgcggccgat caggtcgagg acgaccaaca
      781 tcaccgttcg cgtgaaggtc aaggacacgc gcggctacgt cgtgcgcgac gtctcggtct
      841 tcgtccgcac gaccccgctc gtttcgagaa tccagaacaa ccgctcgcgg accggccagg
      901 acggctgggt cacctacacg gacgtcccgc ggccgaactt ccccgcgatc cgcaacggca
      961 ccaacctcca gtacttcgtc aaggcgtacc gcaacggcga tccgccgctc gccggcgtcg
     1021 ccggctaccg actcgtccag actccgctcc gcaagccgta gttccgctgc ccgcggcggc
     1081 gccgtcaccg agacggcgcc gccgcaagcg gatcgagctc gagcaccctc gcctcggccg
     1141 cctgccgccc ggcaccgctg cgcacgcggg cggccgtccg ccaaacctcg acatcgtcgg
     1201 cgtcgcgctc gatcgacttc gcgctgcttc gcgccgccgc tgcgcggtcg ccgagcgcca
     1261 gctccgcctc ggcccggatc cgccacggct ccgccgccca cggctgcagg cgcgcggccc
     1321 ggcgggcttc gcgcaccgca gcctgggcgt cgccgtcccg aagcgcctcc tccgagcgag
     1381 aaaggagcac gttgccggcg agcaacggca ccgtcagcgc gatcacggtc aggctcgctg
     1441 cgatgctcac gccgcgcggg acgccgatca cgtcgctccg ggccgagacg aggagagccg
     1501 ccgcacagac caatccggcc acggtgacgc cgggcagctc ccagtcccaa tcggcggcga
     1561 agtgaaccag caaggccacc cacgcggctg ccgcagcggc gacccccggg ctcgcagccg
     1621 cccggccggc ggcgaccagc ggtgcggcgc aggagaccgc gagcagcgcc agcgcgactg
     1681 gtccgagctc gctgagcgcc tcgatgtaca gcgagtgcgc gtcgactgcc gggaagagct
     1741 cggcccggcg ctcgagccac acccggcccc atgttccccc tccgctgccc agcaccggac
     1801 tgtcgaccca ctcggcgagg gaggcgtgcc agtaggcgcg gcgctcgtcg ctcgccgccc
     1861 ccccggcggc gagcgagagt gcgatcccgc cggccgccgc gcgggccagc ccgagcgtcg
     1921 gccggttccg acgggacagc aggcctgcgg ctgctcccgc gcccagcgcg atccacgctc
     1981 cccggctctc ggagagccac agcacggcga cggccgggct gcagccagcc accagggccc
     2041 agcgcatggg gccgctcgtc tcgagcgcga gtccgactcc gagcaggatc gccaccgccg
     2101 cgagcagccc cagcccgttg ccgtagccga tcggcgtcgc ctcctctccc gtcgtctcgc
     2161 ctgctcccca gcggaacgcc aggttccaga ggcagatcgc gagcgacccc gcgaggacgc
     2221 cgaggagcgt ggcccggacg agctctcgcc gcaaccacat cagcgcggcg gccaatgccg
     2281 ccaggtagag gagcgttcgc tgcggctcga gcgcggtgag cggcacggca cgcgtccaca
     2341 cgagcgacag gagctgccaa gcgagcagcc cggccagcgc cccgagtgcc gccaactcga
     2401 cgcgcgagag gccgggcgcg cggaggagcg ccgcggccag ggcgagccag gcgaccccga
     2461 acgtggccca accccaggca gacgggaaga agccgccgtc ggccagcccc aggccgaaga
     2521 cgaccgcagc agccgcgagc gcggccgcag ccgcgctaga ggtaggactc ttcgtcgggc
     2581 tgctggagat cctgcggacc gataccggcg acgacggtct cctgcgagcg ctcgtgcagc
     2641 cgctcccgtc ctcgtcgcac ggcgccgagg aacttgtcga ggtttacctc gagagtcgag
     2701 aggatcccgt cggcccagtc ctcgagctcg aggcgtgtct cgcgtgcctg gcgtcgggcg
     2761 tcgtcgacga tctcctgagc ctggttctcg gccagcttga cgatctccgt ctgggacgcc
     2821 tcgcgaacgg cctgctcgcg cgcctcgccg atgagacggt cgcactcccg cttcgcctcg
     2881 gcgagcatct cctgtcgctc cttgacgatc cagcgagcct gcttgatctc ctccgggatc
     2941 gtggcgcgca tctggtcgag gatgtcgtag atctcctccc gatcgatgcg cacctgatcg
     3001 gtgagaggca cggcctttgc gttgtgcacg aggtcgtcca gcttgtcgat gagaacgaga
     3061 acgtccattt gggctccgat cttactcctg cgggctcacc ggggcgccgg gacggccgtc
     3121 cggaaagagc tcccggaacc tgctcgccac cgcctccggc acgaggtcgt ccaccttacc
     3181 gccgaaggag gcgatttcct tcacgccgct cgaggagacg aagctgtagg ctgggctcgc
     3241 catcacgtac atcgtctcga tctcgggcgc gagcaggcgg ttcaggtggt tcatctggaa
     3301 ctcccactcg aagtccgcga tcacgcgcag gcccttcacc atcgtctttg ctccccaccg
     3361 gcgcgcgaag tcgacgacca gctccgaaaa gacgtcgacc ttcacgttct cgaagtcggc
     3421 gagggcgtcc tcgaggaagg cgacgcgctc gtcgatcgag aatgtcgccg acttgtggct
     3481 cgggtcgcgg acgacgccga tcacgacgcg gtcgaagatc gcggccgccc gaccgatgac
     3541 gtcgagatgt ccgttcgtga cagggtcgta ggagcctggg cagatcgcgg tgatcatgcg
     3601 gtcgcgcaga cgctacaggt ctcgcccgac gtgatcgacc aatcttgtaa cagactgtta
     3661 cttgtcgccg gagggcggct tcgggccgtc gtgctcactc tgcgctctga cccggggccg
     3721 gtagagggtg aggcgcgccg agccgtagcg cctgctcgtc acctcctcca gcccgaggct
     3781 cggctccgtg cgagccgccg tctcgagcac gagcagcccc gccggagaca gcaccggcgg
     3841 cagaagctgc tcgagcgccg gctcgagccg cgtgtactcc tcgtacggcg catcgcagag
     3901 aacgaggtcg tagcggcggc tcgccgaacg ctcctcgcga agcacggaca gagcatcgcg
     3961 gcagtggacg accgcgcccg tcaggcgcag cttctcgaga ttggcggaga tcaccttgca
     4021 ggccacgcga tcggcctcgg cgaagacaca gcgtgcggcg cccctcgaga gcgcctcgag
     4081 ccccattgcg cccgagcccg cgaagaggtc gagcaccgtg gctccgtcca cgggcccgat
     4141 caggttgtag gcggcctccc ggacccggtc acccgtcggg cgtgtggctt tgccactggg
     4201 cgcggcgatt cgggctccct tgcggctgcc ggcgatgatc ctcaccgcgg aagggtcgca
     4261 gacgcaccca aggtcggggc cgatctgagg ccgcgctagt tctcgccgag gtgctccgcc
     4321 gcgccgagca gtcggtccac ctcgtcgccg agcagcccct cgctttcgag ctccctcgcc
     4381 acgcgcctcg ctctccgcag caaacccccg tcctgccgga gccgggcgaa gtggagatcc
     4441 gacaggcccg actgtcggcc gccgagcagc tggccctcac cgcgaatctc cagatccttc
     4501 tcggcaagct cgaagccgtc ggcgctggaa accatcgcct cgaggcgctc acgagcgttg
     4561 tccgtcagca tttccttcgg cgcggagacc aacaggcagt acgactgctc ggcgcctcgt
     4621 ccgacccggc cgcgcagctg gtggagctgc gcgagcccga agcggtcggc ttcctgcacg
     4681 atcatgatcg tcgcattcgg gacgtccacg ccgacctcga tcaccgtcgt cgcgacgagc
     4741 acgtcgagct caccggcctt gaactgtctc atcagctccc tgcggtcgcc cgtgcggagc
     4801 cggccgtgca gacagcccac gcggtagccc gacagctctg cgccccgaag tcgctccgcc
     4861 tccgcttcgg cggcgcgagc gacgctggtc tcggattcat cgatcagcgg acacacgacg
     4921 taggcctgcc ggccggcgtc gaggtgccgc cgcaaccgct gataggcctc ggagctgcgc
     4981 tccgcggcca cccaggccgt gatcaccggc ttgcgatccg ccgggagcgc tccaatctcg
     5041 gagacggaga gatcgccgta gacggtcagc gccagtgtgc gcgggatcgg cgtggcggtc
     5101 atatgcagga cgtgcggact ccgaccctcc acgagggccg cccgctgctc gacgccgaag
     5161 cgatgctgct cgtcgacgac cgcgaccgcc aggtccgcga actcgacctc acgctgaatc
     5221 aaggcgtgag tgccgaccgc gatgtcgacg tcccccgctt cgatcagtcc gcgcacgtgc
     5281 gcgtgctcct tcgccgggag cgccgatgtc agcagggcga cgcgcacttc gagggcccta
     5341 cacaggtctt cgatggtcag gaagtgctgt tcggcgagcg tctcggtcgg cgccatcagg
     5401 gccccctgcc ggccggcttc caccgcccgc agcagcgcat acaaggccac ggccgtcttg
     5461 cccgacccca catcgccttg caggagtcgc tgcatcggca ctgtgcgcgc aaggtccgcg
     5521 tcgatctccg cagtcgcccc ctcctgggca tcggtcagcg tgaagggaag cacgctgcgg
     5581 tagcgggcgg cgagctcgcc gggaggtggc agcgccaccg ccatggcccc ttcgtgcgcg
     5641 gcggctcgac gggccagtgc gagctggagt acgagcagct cgtcgaaggc aaggcggcgg
     5701 cggccgacct ccgcctcctc gagggacagt ggccggtgga gggcgaccag tgcgtcctgc
     5761 cgcaacggca acccctcggc gagccgcagc ccgaccggaa gccggtcgcc agcggcgccg
     5821 gcgtgcacga gcgcggcctc ggcgatcccg cgcagctgct tctgcgagag ctcttcgctc
     5881 gccgggtaga cgggcgcgaa gtcggcggtc tcggcggcct caccgagatc gtgagactcg
     5941 acgacgaatc cgcgccggcc gttcctgccc cgcagcctga cgcgtgttcc gggagacagc
     6001 tgccgttcga gccagggctg gttgaaccag gtggcgcgaa cgacgccggt ttcgtccgag
     6061 accttggcgg tcagtatgtg cagccggccg tggcgtcgcc ccgacacgtc gagcacctcg
     6121 ccttcgataa ccgcctcctc gtcgccgaag agctcggcga tcgcacgctc ggggactggc
     6181 tgctcgtacc gcctcgggcg gtgtgccaac acgtcgccga ccgtctcgat cccgagccgc
     6241 cgcagcttcg ccgctaccgc cgcccccacc cgcgggagcg tcgtcaccgg cggggagagc
     6301 gcctccggcc gaggggccga gcgcggtctg ggccatgtgt cgggcggctc cgcaccggca
     6361 aagcccgccg ggagccgggt tcgtgtcgtg gtcgccacct ccggaccgag tataggaccc
     6421 gggtccggac gtctcgcggc cgtcagactt cgtcttggaa ccagaagtag ccgacggccc
     6481 cgggcccggc atgggtgccg acgaccgcgc cgagcgtctc gaccagcgcg atctcggcgt
     6541 cggcacgcac cttgtgggtc atgtccgtga gcacgtcgac ccagtccggc gcgttcgcat
     6601 gggcgatcgc gacgcggatc cccggcccct cgtgcgtgcc cgcctcgaac gcccgcgcga
     6661 actccttcag cgccttctgt ctaccgcgga cgcgggcgac ggcagccacc tctccgtcgg
     6721 ccacgccgag tatgggcttg acgttcaaga gcgcaccggc gaaggcctgt cccttgccga
     6781 tccgtccgcc cttctgcagg tattcgagcg tgcccaccgt gaagaccacg ccgttcacct
     6841 gcttgaagcg gtcgaccagg ctctcgatct cctcgtcaga cgacccgcgc ccaaggcggt
     6901 gctggactgc cagcgcgagg agcgcgacgg cgagggacgc cgtctccgag tcgacgacgc
     6961 ggacgacgtc gccgccgagc tcgtccgccg cgacgcaggc cgactggtgt gtccccgaca
     7021 gcttcgccga gagatggagc gaatagatgc gttcgaagcc cgcctcgacc agctcctggt
     7081 agacggagag gaagtcctgc ggcgtcggct gcgacgtcgt ggggagctcg gatgccaccg
     7141 cgagccgttc gtagaaggtc gtcggatcga tgtcgacgtg atcccgcagg ctgtcgtcgc
     7201 cgaaccggac gtacagcggc acctcacgca ggttcccgaa gcggttcgcg gcgtcgggga
     7261 agtccgacgt cgagtcgaga acgatcgctg tgttctctcg cgtcagggtc actgcgctcc
     7321 ctctcgtgcg gcgtgtttga ttgcggcgac gagccgctcg atcggctcgc ccttctcgac
     7381 gaaccctacg gcaccggccg cccggacgga ctcgcgctcg tcgtcggtgg cctcggcggt
     7441 gagacagagg acggcggccg ccggccgcga cgccagcact gcggcagtgg cctcggcacc
     7501 gtcgagtcca ggtagccgga aatccatcaa cgcgacgtcg acctccaggt tcacggcggc
     7561 gtccgcggcg cttctcccgt ccgcaatgct gccgacgatc tcgaggcccg gctgcatgcc
     7621 gagcaggagc tcgagcgacg atcggtacac gtcgttgtcc tcgacgagca ggacccggat
     7681 ggcagcttgc tcgctcacac ggcggcgagc agcagcgggt agtgaggctg gccgccgtcg
     7741 cgaggatcga ccgtcacgtg agggtgtcgc tgctcgatgc gtgcccgcag ccggtcgacg
     7801 tcgggcgcgt cctcgccgac gagaatcgtg agcgtctccc ggccatccgc aacgagccgc
     7861 tccaccacgg catccacgac cgcatcgaac tcctcgccgc aggtgaccac tctgtcgtcc
     7921 acgagtccca gccaggcgcc tgcgcgcacc tcgacaccgt cgatctccgt gtcgcgcgag
     7981 gcgcgtgtga cctcacccgt cacgacttcc gcgacggcct cgagcatcgc ctcctcgttc
     8041 tcggcggcgg cgagcccccg gttgaaggac accatcgcct cgatcccggc ctgaacggag
     8101 gtcgtcggga tcaccaggac ggtcttgtcg gagtgctccg ccgcatgctc cgcgctgagc
     8161 aggacgttct tgttgttcgg cagaacgatg acgtcgtcgg ccggcgccgc ctcgacggcg
     8221 tccaggatct cggccgtgga cggattcatc gtctgaccgc cttcgatcac gcgggttgca
     8281 cccagcgact cgaacagacg gcggttgccg tcccccgggg ccaccgcgac gagcgcggtg
     8341 accagagtcg ggatgtcggc cagctcgtcg gaaagccttt cctcccgctc ttgcgtttgc
     8401 tcgtgcatgt tcgcgatctc gatcccgtcg atcgtgccga cagccgtgcc cacgctgaga
     8461 gccgcgccgg gatcgtccgt atgaacatgc accttgaggg cggtggcgtc gccgacgacc
     8521 agcagcgagt cgccgagctg ctcgagctgc tgctcgagcg cgtcgaggtc gaggccctca
     8581 ccctcgacga cgaacacggt gcagtagcga aaccgggaca gctcctggtg gatcgcgtcc
     8641 acgccgagct cctcagcgtc gaccggcgcg tcggggatcg gttcgcccga gacgccgagc
     8701 gcgattccgc gcacgatctc gacgaggcct gcgccacccg catcgaccac cccggccttc
     8761 gcgagcacgg ggagcatctc gggcgtgcgc gccagcgcct gctcgccgcg ctcgaccgcc
     8821 cgccagagcg cctcctcctt gcccaacgct cgcacgtccg gcagctcggc ctcctccgcc
     8881 atttcgcgca cgaccgtgag catcgtgccc tcgaccgggc gtcgcacggc tcggtaggcg
     8941 gcgtcgctcg acgcgcgaaa agccttcgcc agcacctcgc cgtccacgtc gtcgtggcgg
     9001 ccgagcacct cggcgaagcc acgcaggatc tgcgagaaga tgaccccgga gttcccccgc
     9061 gcccccatca gcgcggcccg cgagagctcc tttgcgaccg gctccgggcc ctcggccgtc
     9121 gagctgccga gcgcctcgac gacggcgcgc actgtgagag tcaggttcgt gccggtgtcg
     9181 ccgtcgggaa ccggatagac gttgaggtcg tcgatgtgct gccggtgcgc ctcgaggttt
     9241 gccagcgctg cggcggcgag ctcgttggcg cgcgccacgt tcatgaagac ctcctgacgt
     9301 cgtcgatcct cacatcgacc gatgcgacct tcagtcccgt ttgacgctcg acctcgtacg
     9361 cgacgcggga cctgagtgtc gaggcgatct ccgccaggtt gaggccgtac tcgaccacga
     9421 cgtggagcac gagctcgagt ccatcccgat ctccgcgcac gtcgataccg ctcgtgagtg
     9481 ggtctcgcgg gcgaagccga agccgccgac cggccatctc gacgacgccg tacgcctcgg
     9541 ccgcgacgtg acccacgatc tgctggatcg cctcggtcga gacgccgacc cgcccgagct
     9601 cggacgaggc gacaatgtgg attcccttgt ccatgtcgcg acgttagacg ccgcgagcgg
     9661 gcgatgggtt acgccgagcg ccgggcgcgc gcggcaaagc ggctctagac ggccttctcg
     9721 accttgccgg ccttgaggca ccgcgtgcag acgtaggcgc gtttcggctt gccggccagc
     9781 agcacgcgta cccgctgcag attgggattg aagcgccgcc tggtcgcaac catcgagtgg
     9841 ctgcgattgt tcccgaacga cggcttcttc ccgcagatcg cacagacctt cgacatccgg
     9901 ctgggaagcg tagccgaact actgcgccgc ggcggtacgg aggttcgcga agacgtcggc
     9961 catctcgaga gccgtccgca cggcctccgc gcctttgtcc accctggtct ccgcctggtc
    10021 ggcgtcctcc agcgtgagaa caccgaacga gacgggaacg ccggtttcca gtccggcgag
    10081 ttgaaggccg ctcgccgcct ctcctgccac gtagtcgaag tgcggggtgt ccccgcggac
    10141 gacgcagccg agggcgacga cgcaggagaa gcgccgcgtc ttggcgagcg ccatcgccgc
    10201 gaggggcagc tcgaacgcac ccggcaccgg catcacggtg atggcttctc cggtgacgcc
    10261 tcgttcttcg agctcagcga gggcgctttg gaggagcctc gtcgtgatgt caccgttgaa
    10321 gcggctgacg accacgccga cggcacgacg cgcaccgttg ggctggccct cgagcaccgc
    10381 gtagccgggc ggcaccgcga acgttccggg agcgtgctcg ctcgcgggca cttcggcctc
    10441 cgcgccgggc acatcgtcgg gctccgcttc gggggcgggg gctgacgggg tctcggcggg
    10501 aggggcgctc gccgccacgg cggcggccgc cacggttagc tcgcgctcgt ccaaggagat
    10561 ctcctccgcc aacccagctt ctacgggctc tgcatcgccg gccgcgaccg cctcctcgac
    10621 caccggcgca gcatcggtcg acgactcgga agcacgctct ggctcgcttt ccggctccgg
    10681 cccggccccg agaccgccgt caagctcagg ccctccgtcc tcctcgtccg gccggatggg
    10741 ccaaccctcg aggacgttct cacgggccag gtcgatgtcg tccttgccct cgggctcttc
    10801 ctcggaggcc gggcgcgcat cctcgtcgga gtcctcccag cgggcgtgat cgtacggccc
    10861 cgtgggggcc cgtttgtgat cggtttcgtg gcggtcgctc aaagctcagc cccctcgaac
    10921 aggtcggggt cgaagcggag acgttgatga tgaagcttgc ggccgatggt atgcccaagc
    10981 ttgtcacgct tcacggccag ataccgccga ttctgctcgt tcggctgtac ctcgatcggc
    11041 tcctgggaga cgacggtgag cccgtaaccg gcaacacccg cgatcttctt cgggttgttg
    11101 gtgaggatgc ggatcgaggt cagtcccagc tccgcgagga tctgtgagcc gatcccgtac
    11161 tcgcgcgcgt cggccggaag tccgagctcg atgttcgcct cgacggtgtc gcggccgttc
    11221 tcctggagct cgtatgcgcg gagcttgttc aggaggccga tcccacggcc ttcctgtgcg
    11281 aggtagagga gtacgccgcg tccctcctgc tcgatacgcg ccagcgcgag ttcgagctgc
    11341 tcgccgcagt cgcagcggag cgagtggaag acgtcgccgg tgagacactc cgagtgcacg
    11401 cggacgagca cgccgtcttt cccctcgacg tcacctttga cgagggcgac gtgctgcttc
    11461 ccggtcagga gctcgcgaaa cgcaacggcg gtgaagtcac cgtactgcgt cggcaggcgg
    11521 accgagacga cgcgctccac gagcttctcc gttcgccgcc tgtactcgat caggtctgcg
    11581 accgtgatca tcttgagccc gtgccgcccg cagtactcga cgaggtcggg cacgcgtgcc
    11641 atcgtcccgt cgtccttcat gatctcgcag accaccccgg ccggtgtcag cccggccaat
    11701 cgcgccaggt cgacggcggc ttcggtctgg cccgcgcgct ggagcacgcc gccgggccga
    11761 gcctgcagcg ggaagacgtg tccgggtgag acaacatcgc gggcggttgc ctccggtgcg
    11821 atggccactt ggatcgtccg ggcccggtcg tgtgccgaga tacccgtgga gactccctcc
    11881 ctggcttcga tcgagacggt gaacgcagtt cccagcggcg tctcgttctg ctcggtcatc
    11941 tgccgcaggc ccaactcggc gcagcgttcg cccgtcaggc agaggcagat caagccgcgg
    12001 ccgtgggtcg ccatgaagtt gactgcctcg ggcgtcgtga actccgccgc gatggtgagg
    12061 tcgccctcgt tctcgcgatc ctcgtcgtcg acgacgacga cgaaacgtcc cgcgcggacg
    12121 tcctcgatcg cttcctcgat ggaggaaaag ggcgtctcct cgcccacgag cgcgggctca
    12181 ctgctcatgt agtggatggt aaccgtggct aggcctcggg catcgagagg agccgtgccg
    12241 tgtacttggc gagcacgtcg ttctcgaggt tcacccgccg gccgggtgcg aggcgcgaga
    12301 gcgtcgtgcc ttcgagcgtg tgtgggatca gcgcaacgcc gaacgcgtcg tcggcgacgt
    12361 ccgtgacggt aagagagacg ccgtccacgg tgatcgaccc cttctccacg cagtagcgaa
    12421 gaatgtccga cgacgcttcg acgaagaccc ggaggccctc cccctccgcc tcgaccgact
    12481 gcacgcggcc cacgccgtcg acgtgcccct ggacgtagtg gccgccgagc gggtctccaa
    12541 ccctcagtgc cggctcgacg ttcacctcgt cgccctcggc caggtcgccg agcgtcgtgc
    12601 ggcgaagcgt ctccggaacc gcgtggaacg cgatcagccc gccgtcggcg acctccgcgg
    12661 tgaggcagca cccgttcacg gagacgctgt ctccgatgcc gatctcgagc gcgatgcgag
    12721 gggtctccac gaagatcctg gcgccgtcgg cgtcgcttcc ttcgatccgt gcaatccggc
    12781 cgagctccct gacgatccct gtgaacacgc gcagaggcta tcgcggttcg tgcaggtaag
    12841 cctcgagcat gacgtcgtcg ccgacgtgca ccgacgtgag gtgcgagagc tgtatcgggc
    12901 cgcgcaacgg gccgagcaga tgcggcccgt cgccggagag cgtcggcgct acgaagagca
    12961 gcagcttgtc gacgaagccc gcgtcgagaa acgcggtcgc gagcgtcggg ccaccctcga
    13021 gcaggagtga ctggacgccc tcgtcaccga gcgcccgcag ctcgtcgtcg agcggccccg
    13081 agcggagctc gagcgtcgag tcctccggca gcggtccggc gccgaacgcc aaccgccgcg
    13141 gctggcggac ggcatcgacg gcccgagcgt cgagccgagg gtcgtcggcg cgcaccgttc
    13201 ccatgccgac cgcgacggcg tccgagcggg cgcgaagctc gtgcacgacc cgcctcgaag
    13261 cctcaccggt gacccagcga gatccgggta ccgtgacgcg accgtcgacc gtgaccgcgg
    13321 ctttgtacgt cacgaagggt cgtttttagg cgatccaggt gcgccaggcc tcgttctggc
    13381 gtcgtgcctc gaacgaatcg tcgacgcgga cgtcgacgcc ggctgctcgc aatcgctcga
    13441 cgccgtcgac gtcccggttc gggtcgaggg ccccgatcac cacagaggcc acgccggccg
    13501 cgatcagggc gtcgacgcac ggcggcgtgc gtccgtggtg cgagcagggc tcgagcgaca
    13561 cgtacagcgt cgcgtctcgc gcgcgctctc cggccgcctc gagcgcgacg atttcggcgt
    13621 gcgcaccgcc ggcccgctcg tgccagccat cgccgacgac cactccgtcg cgtacgagca
    13681 cggcgcccac gaccggattg gggtgagtcg ttcccctacc ccgctcggcc agctcgaggg
    13741 cgcgcgctac gctcatcgtg cggctgcggc gatccggcga taggcggcca ccagatcggg
    13801 atcggcgaag atcgcgctgc cggccacgag ggcggaggcc ccggccgagc gcagagcgcc
    13861 cgcgttcgtc tcgtccacgc ctccgtcgac ctggatcggc aggtcgacga gctcgcgcag
    13921 tcgagcgacc cgtccgaccg atccgggtat gaacgcctgg ccggagtagc cgggccatac
    13981 gctcatcacg aggatcatct cggcggcggc ggagcgggcg aaggccgcgg ccacctccgg
    14041 ctcggtgccg ggattgaaca ctgcgccgac ggcgagtccg tgctcgcgcg cctgcgccgc
    14101 gaccccggcc gggtcgtccg tggcctcgac gtgaaacgtc accgagtcag cgccggactg
    14161 ggcgaactcg gcgaagtggt gagcagggtc ggcgaccatt agatggcagt cgagcaccgc
    14221 tcccgccgcg tgcaccagcg gagcgatcga ccgcagcacg actggaccga tcgtgaccgg
    14281 cgggatgaag tgcccgtcac ccacatcgaa gtgaaagatg cggacgccgg ccgcgagcag
    14341 cgaccggatc tgctcgccga gacgggagaa gtcggccgca tacaacgacg gctcgatctc
    14401 gacgtcatcg cggatccagt cggcccagct caccgcgcga tcttatgtcg cgcggagccg
    14461 gtgttcgctt ggcgcagcct ctggagaggc gtaccgtggt cgaatgggcg tgcacgtcgc
    14521 tgtggaaacc aggatcgacc gcagacaaga cgacgttcgc gcctacgtca tcgatccggc
    14581 gaacgacccg gagtggatcg gcggcatcac cgaggcgcgg ctacttaccg aaggcgacgt
    14641 ccggttgggc tcgcgcgttg cccgcgtcgc ctcgttcatg gggaggcgca tcgagtacgt
    14701 caacgaggtc acggtgctga ccgacgacga gctgactatg cgatcggtcg cgggcccctt
    14761 tcccatggag atcacgtacc gcttcgatcg ggagggagag gggactcgag tcggcgtccg
    14821 agtgaggggc gaaacgggcc gtttctatcg aatcgccgag ccgctcctgg cccggcgggt
    14881 ccgcagctcg attaccgagg acgtcgagag gctacgcagc atcctggagt cggctccggt
    14941 cagctgaccc gaagccgcgc tacgaagaag ccgctggtgc cgtgaacgtg cggaagcgtc
    15001 tggaggaagt ccggccgccg cggatgccga aaacgcggcc actcgtcggc cagcgacgga
    15061 tcgacctcca agcgggcggc gtccacgacc gcctcgctct cgtcggcgtt gaccgtgcac
    15121 accgagtacg tcaccgtccc gccgtgccgc acgctggcgc ccgccgcacg cagcagctcg
    15181 agctggagct cgggcagagg ctttccgcgc cagcgcaggt ccggccgggc ggcgagcacg
    15241 cccagacccg agcagggggc atcgacgagg gcgtggtcga actcaccgag gtcgggcgga
    15301 agagctcgcg catcggccac gaggacgctg acgttcgccg ctcccagtcg acgtgccgtc
    15361 tcctgcaact gccgggcacg tccttcgtgc acctcgacgg cgagaacctc gcccgccagc
    15421 atcgtcgcct tgccacccgg cgccgcacac aggtcgagcg tccgttcccc gtcacgcgcg
    15481 ccgaccgcga gacccgcgag ctgggagcct cggctctggg gccagatccg cccctccgcc
    15541 agcgcggcct cgtcgacccg ctcgacgtgc caggcacccg ggatctccgt atccggcgtg
    15601 ccgtcgaccg agccacgaca gaggcgcacg acggtctctg gcggctcgtt ctgagcctgc
    15661 atcagccgca ccgcgtccgc cgcgccgaga tcgcgccacc acacctccgc aacccagtcc
    15721 ggatacgagt gccgcagtgc ggcctcgacg ggagtgcgat ccggcaagcc tcggatgagg
    15781 tcggcgatac cgtccgcgat tcggcgcaga acggcgttgg tgaagggcac cgcgcgctcg
    15841 agaccggctc gacgaacgag ctcgaccgac tcgttgactg ccgcgtagcg agggacaccg
    15901 tccaggaagc cgagctggta agcgccgagc cgcagggcgg ctcgaacggg agggtcgagc
    15961 ttcggcaccg gacgacgacc gagccgctcg atcgcgtgat cgagcgttcg gacgcgctgc
    16021 acggcaccgt aggagagctg ctgcgccagc cgccggtcgc gctcctcgag accgtcgact
    16081 gccccgcgca gggcgcggtc ggtgtaggcc gcgtcttcga agacgcggat cagcacgtca
    16141 tacgcggcgc gtcgcgccgg agcgatcacc gaaggccgcg aagccattcc tcgtagccca
    16201 tgcggcgttt tccgtcgggc tgcacctcga gaggggcgaa cgagccgtcc tccgcgacgc
    16261 gggcccgcca caccgtcacc ggccgacccc gcagctcagc ccgagcgccg atgtgcggcg
    16321 agagcgcgcg cacctgccga acgagctcgt tcgccggccg cgacaggtcg agcgagcgat
    16381 cgcccggacc gatcttgtct gcgtacgtga ccccgacgtc ggactgggcc acgaaggacg
    16441 ggctcggatc ggcgagcacg ccgtcgagca gctcgctcgc gacggcggcc gcgcgcgcgt
    16501 agacgtcgcc ggcgtcgtcg tccggcccga tcgggaagga gcgctgggcc gcgacggggc
    16561 cggcgtccag gttcttcacg gtctcgtgga tcgtgacccc cgtttccaga tcaccggcca
    16621 tgatcgatcg ctcgacaggc gcagcacccc gccagcgcgg gagcaaggac ggatgcacgt
    16681 tgagccacag ccgttccgag aggagtccct ccgggatgat caagccgtac gcgcacacca
    16741 ccacgatccg cgcgtcgagc tcgagacctg ctttcggctt cgcgagctgg agcacgcgga
    16801 gtccgaggct ctccgccgcc tccttcgccg gtggcgcgcc gagcttgcgg cctcgccccc
    16861 tgggtgcgtc cgggcgggtc agaaccgccg tgacctcatg ccgggcggca agtcgtgtga
    16921 gcacgtcggc gccgaacgcg gccgtggcgg cgacggcga
//
LOCUS       DBNGNK010000120         3018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1095745,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000120 DBNGNK010000000
VERSION     DBNGNK010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1095745"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC150V_03500"
     CDS             complement(<1..201)
                     /locus_tag="AC150V_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3762016.1"
                     /translation="MCGIAGALSWWADRAVRAEEITRMRETLIHRGPDGGETWVADDG
                     RIGLGFRRLSIIDLSDSAMQPMA"
     gene            complement(201..1307)
                     /gene="wecB"
                     /locus_tag="AC150V_03505"
     CDS             complement(201..1307)
                     /gene="wecB"
                     /locus_tag="AC150V_03505"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011423222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR3762017.1"
                     /translation="MSRLVCFVVGARPNFMKAAPVYFALREREEISTLLIHTGQHYDR
                     EMSDVFLGELGLPRPDVFLGVGSGTHAEQTARALTGVESVLVEHEPDLVVVPGDVNST
                     LAGALAAAKLRVPVSHLEAGLRSYDPDMPEEHNRRLTDHLSDLLLVHSESAIANLRRE
                     GITAGIEFVGNTMIDSLLTHVERARALAPWRDLEVERGGYALVTLHRPALVDRPALLR
                     DVMDGLAALAHEMPVLFPVHPRTQGRIREERVPTPPGVTLAEPLPYLSFLGLEAEAAV
                     VLTDSGGVQEETSALGVRCFTLRDTTERPVTVELGTNTVLGARPERIAEIPELLLEPK
                     PGSPIPLWDGRAGVRAAEAVASFLADRAEVGARV"
     gene            1377..2483
                     /locus_tag="AC150V_03510"
     CDS             1377..2483
                     /locus_tag="AC150V_03510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3762018.1"
                     /translation="MLRKALFWGSLGALAWTHAGYPLAARGAAQLLRRPVRKDTGAEP
                     AVSVIVAAHNEESVIAGRLENLLALDYPPEKLEVVVASDASSDRTDEIVAAIAEREPR
                     VRLIECARAGKVAAQDRAVRETSSDVVAFSDANASWAPDALRRLVANLADPEVAYVCG
                     QLKLQAADGSNREGLYWRYEMALRADESILGSVTGGNGSIYALNRDDYLEVDPRHGHD
                     LSFPYRLVQRRRRAVYEPEALAFERPTPSNEAEYRRKVRMFEHCWLMTLQGSMLRDLP
                     PGYLVQILTHRVLRYASGVLHVVLLGSSVLLAGAGPFYRITLAGQLALLAAAAKGQPI
                     ARYYTLITWATAQALFNYLRRGVPATWDAAEGTR"
     gene            2480..>3018
                     /locus_tag="AC150V_03515"
     CDS             2480..>3018
                     /locus_tag="AC150V_03515"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3762019.1"
                     /translation="MNRALDVVLAGAGLVLTSPLLAAAAVATKLEDGGPVLYRQTRVG
                     RNGVDFELVKLRTMVVGAEHQGAGFAVNTGDPRITRSGRLFRRTSIDELPQLWNILRG
                     DMSVIGPRPTLRYQVEKYDERQRRRLDVKPGLTGWAQVKRGYGRSEGDALEKLQYEFY
                     YLRRQAFAVVIRILFRTLRT"
BASE COUNT          443 a         1067 c         1065 g          443 t
ORIGIN      
        1 cgccatcggc tgcatcgccg agtcggagag gtcgatgatc gagagccggc ggaagccgag
       61 cccgatccgg ccgtcgtcgg cgacccacgt ctcccctccg tcggggccgc ggtggatcag
      121 cgtctcccgc atgcgggtga tctcctccgc ccgcaccgcc ctgtcggccc accatgagag
      181 cgcgccggcg atgccgcaca tcagacgcgg gcgccgactt cggcgcgatc ggcgaggaag
      241 gaggcgacgg cctccgccgc ccgcaccccg gcacgtccgt cccacagcgg gatcggcgat
      301 cccggcttcg gctcgagcag cagctccggg atctcggcga tccgctccgg tcgcgcgccg
      361 agcaccgtgt tcgtgccgag ctcgaccgtc acgggtcgct cggtggtgtc gcgcagcgtg
      421 aagcaacgaa caccgagcgc cgaggtctcc tcctggacgc cgcccgagtc ggtgagcacg
      481 accgccgctt cggcctcgag accgaggaag ctgaggtacg ggagtggctc ggcgagggtg
      541 acgccgggcg gcgtgggcac gcgctcctcc cttatacggc cctgtgttct cgggtgcaca
      601 gggaagagga cgggcatctc gtgggcgagc gccgcgagtc cgtccatcac gtcgcgtagc
      661 aacgccgggc ggtcgacgag ggcaggccgg tgcagcgtca cgagtgcgta gccgccgcgc
      721 tcgacctcca gatcgcgcca cggcgccagc gcacgcgccc gctcgacgtg ggtcaggagc
      781 gagtcgatca tcgtgttgcc gacgaactcg atgcctgccg tgatcccctc ccgccggagg
      841 ttcgcgatcg cgctctccga atgcacgagc aggaggtccg acaggtggtc ggtcagccgg
      901 cgattgtgct cctccggcat gtccgggtcg taggagcgca gtccggcctc gaggtgcgag
      961 accggaacgc ggagcttcgc cgcggccagc gctccggcga gcgtggagtt gacgtcgccg
     1021 ggcacgacga cgaggtctgg ctcgtgctcg acgagcacgc tctcgacccc ggtgagcgcc
     1081 ctcgccgtct gctctgcatg ggtgcctgag cccacgccta ggaacacgtc gggtcggggc
     1141 agcccgagct cgccgaggaa gacgtccgac atctctcggt cgtagtgctg acccgtgtgt
     1201 atgaggaggg tgctgatctc ctcgcgctcg cggagcgcga agtacaccgg agcggccttc
     1261 atgaagttcg ggcgcgcgcc gacgacgaag cagacgagcc tcgacatcgc ggtcaaggct
     1321 agatgatcag ccggaaacgc ggccgcgcgg cgtgtaaccg cgactacact cgcgcggtgc
     1381 tccgcaaggc gctcttctgg gggtcgctcg gcgcgctcgc gtggacgcac gcgggctacc
     1441 cgctcgctgc gcgcggggcc gcacagctgc tgcgccgccc ggtccggaaa gacaccggtg
     1501 ccgagccggc ggtcagcgtg atcgtggcgg cccacaacga ggagtcggtg atcgcgggtc
     1561 gcctcgagaa tctgcttgcg ctcgactacc cgcccgagaa gctcgaggtc gtcgtggcct
     1621 ccgacgcgtc gagcgaccgg accgacgaga tcgtcgcggc gatcgcggag cgcgagccgc
     1681 gcgtgcggct gatcgagtgc gcgcgcgccg gcaaggtcgc ggcgcaggat cgcgccgtgc
     1741 gcgagacgtc gagtgacgtc gtcgccttct ccgacgccaa cgcgagttgg gcgccggacg
     1801 cgctgcgccg gctcgtcgcc aacctcgccg atcccgaggt cgcctacgtg tgcggccagc
     1861 tcaagctgca ggctgcggat ggctcgaatc gggaggggct ctactggcgc tacgagatgg
     1921 cgctgcgggc ggacgagtcg atcctcgggt cggtcacggg cggcaacggc tcgatctatg
     1981 cgctgaaccg tgacgactac ctggaggtcg acccacggca cggccacgac ctctcgtttc
     2041 cgtaccgtct ggtacagcgt aggaggcgtg ccgtgtacga gccggaggcg ctcgccttcg
     2101 agcgcccgac gccgtccaac gaggccgagt accggcgcaa agtgcgcatg ttcgagcact
     2161 gctggctgat gacgctgcag ggctcgatgc tgcgcgacct cccgcccggc tacctcgtcc
     2221 agatcctcac gcaccgtgtt ctccggtacg cgagcggggt gctgcacgta gtcctgctcg
     2281 ggtcgagcgt cctgctcgcc ggcgcggggc ccttctaccg gatcacgctc gccggccaac
     2341 tcgcgctgct cgccgcggcc gcgaaaggcc agccaatcgc ccgctactac acgctgatca
     2401 cgtgggcgac cgcgcaggcg ctcttcaact acctccggcg cggcgtgccg gcgacgtggg
     2461 acgcggccga gggaacccga tgaaccgtgc tctcgacgtc gtcctcgccg gcgccggtct
     2521 cgtcctcacg agcccgttgc tggcagcagc cgccgtcgcg accaagctcg aggacggcgg
     2581 tccggttctc taccggcaga cgcgggtggg cagaaacgga gtcgacttcg agctcgtcaa
     2641 gctccgcacg atggtcgtcg gggccgagca ccagggcgcc ggcttcgccg tcaacaccgg
     2701 tgacccgcgc atcacgcgga gcggccgact gtttcggcgc acctcgatcg acgagctgcc
     2761 gcagctgtgg aacatcctcc gcggcgacat gtccgtgatc ggcccccggc cgactctccg
     2821 ctaccaggtc gagaagtacg acgagcggca gcggcgccgt ctcgacgtca agccgggcct
     2881 caccgggtgg gcgcaggtga agcggggcta cggacgcagc gagggcgacg ccctcgagaa
     2941 gctccagtac gagttctact acctgcgccg tcaggccttc gccgtcgtca tccgcatcct
     3001 cttccgcacc ctgcggac
//
LOCUS       DBNGNK010000121         7142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_592489,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000121 DBNGNK010000000
VERSION     DBNGNK010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_592489"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            772..1413
                     /locus_tag="AC150V_03520"
     CDS             772..1413
                     /locus_tag="AC150V_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762020.1"
                     /translation="MRKLIVSVACALGATVMLSQAALAQDDEGERVCRGFVVHGVVPE
                     VGERTIVVAADRARPDGLAGTEVSLRLGSRTRMDGEGPVEVGSSVKARGTVCQVDEAD
                     EPVFYAKRVKVKRVRAERLRGAFELAGTVTDVSEDGFAVEVSESSFASLVGTIVSIGF
                     RNRTTVEGELAVGVDVVVAGRARIRGGESVLLAHEVTVMAGDEEPEPPPDGGE"
     gene            1477..1950
                     /locus_tag="AC150V_03525"
     CDS             1477..1950
                     /locus_tag="AC150V_03525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR3762021.1"
                     /translation="MRIPDLGSRGQGWVYLQFVLIAAVVVAGFAPPRWPEGLARPFDL
                     AGAVIALGSGFIAVWAARTLGRSLTPYPAPADGGVLVAAGPYRFVRHPVYAAGIGFFL
                     GVALVTGPWALVPTAVLSVVWALKLQVEERFLGERFPDYARYCEQVPARLVPGIF"
     gene            1972..2499
                     /locus_tag="AC150V_03530"
     CDS             1972..2499
                     /locus_tag="AC150V_03530"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR3762022.1"
                     /translation="MTASWPEPVERVAAHLRASGAEGRIEELPAETPTAQAAADAVGC
                     GLAQIVKSLVLVGASGTYFMALVPGDRRADLAKIAAAVGTTKVRVARPDEVVEATGFS
                     PGAVAPFPVPRVTRSVVDQSILAQRAVWIGAGSTRHLACLAPAELVRLSRADTVDVIE
                     APEYHSQSESETKES"
     gene            2500..3429
                     /locus_tag="AC150V_03535"
     CDS             2500..3429
                     /locus_tag="AC150V_03535"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013347149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR3762023.1"
                     /translation="MQATEKIWMNGELVDWDDAKIHVGVHGLHYGTGVFEGIRCYETP
                     KGPAVFRLAEHMQRLHDSARLLYMGIPFSVEELRQATHDLVRVNGLPSSYIRPIAFYG
                     YGELGVHTGGNPVETVIMNWPWGAYLGEDSLQKGITAKISSWTRVGPNVIPHVAKATG
                     IYLNSMLATSEALRAGYNEAILLTDHGTVADGPGENIFVVKDGVIFTPPLSTSILPGI
                     TRDSVMTIAREQGYVLEERDIIRTDLYLADEVFMTGTAAEVTPVRAVDDVEIGVGPVT
                     LALQKTYLDMVNGVDEPWGDWLDVVETPARAEA"
     gene            3426..4580
                     /locus_tag="AC150V_03540"
     CDS             3426..4580
                     /locus_tag="AC150V_03540"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3762024.1"
                     /translation="MSAATRQIPLSAPWLDDREEELVLDVLRSGRLSLGPAIERFEEL
                     FAQTVGAPYAAAVSSGTAGLHLLCRSAGLAPGDEAITSPYSFVASANCAIYEGATPVF
                     ADIDPGTLNLDPATVEAVVTERTRAIIAVDIFGYPCELEPLREICDRYGLVLIQDACE
                     ALGAQYRGAPVGSHGPSSVFAFYPNKQITTGEGGIVATHSEDEWRLLKSLRNQGRADG
                     GGWLDHTLLGFNYRIDDIRAAIGIGQLERLDRILAARAKVATRYGDLLSAIPGLELPL
                     ADDAEHVRSWFVYVVTLPPEVQREQVIAALTERGVQTARYLPCIHLQSYMRERYGFAE
                     GLCPVAEGLSSRTLALPFHARLSPDDQDYVVEALEASLELVAAAGSSASR"
     gene            complement(4511..5857)
                     /locus_tag="AC150V_03545"
     CDS             complement(4511..5857)
                     /locus_tag="AC150V_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762025.1"
                     /translation="MTAVTTPLPRHTRILYASASLGGEALTQSRNLWLVYYYAPPEDA
                     DLPELLSPLAVGVILTLVRLAESLDDVVVGWWSDRTESRLGRRLPFIVLATPFVGLFA
                     ALLFMPPADGTGAALWLAVTLFGYFLFSTLSSGPYEALFPEIAQTTEDRLAVVGTRVY
                     FGAAGGAIGLVGASLLIDSVGFAAMATVMAVIAVGFRYLGTAGAWRRASRTTPPALIS
                     LRQALRLTVANRSFLLFLPSFVLFQVGVQLLLADLPFFVKAVLDVEEEDRWVAGLTAV
                     AIAVVLVSVPFLTRAARRTSKRRVYLASMLGASLLFPLLGLVGIVPFVPAEVQLVVLI
                     AAVGVPIAGAFLFPAALTADILDDDQTRSGLRREATYFGAQNLVEKTVTSLTPLLLGL
                     LLILGRTTESQAGVRVAGLVAGAIVFGGFLVFRRYRLADDPAAATSSSDASSASTT"
     gene            5921..6973
                     /locus_tag="AC150V_03550"
     CDS             5921..6973
                     /locus_tag="AC150V_03550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR3762026.1"
                     /translation="MILGVVLDRPVVGSALRGLTGTPAISEVRADDVPATLAVPAGRG
                     PWPAAVFVNGVTARGRGHPQVRRLAEGLARAGVLALVPEPAGLATGELSPTTIEQTVA
                     ASLAVADRPDALAGSVALFGASAGTALALIAAQQDTLRERVSVVAGTVPYAHLREAAR
                     VATTATYALDGRRRPWQAPRFLGLVIARSIVAALRASQTRDRLRDALLALPDDTADPF
                     APFREWPKAPHARAVAALLTNEDPVRFETLYDDLPEYLRDGLDRLSPLPGASAVAAPV
                     ELVSAEQDSYFPLADGRALAAATGGRLTVTRALRHAVPKPTPSGLADVGRLYDWATRS
                     LAAAGRVTSPPSPVRP"
     gene            complement(6942..>7142)
                     /locus_tag="AC150V_03555"
     CDS             complement(6942..>7142)
                     /locus_tag="AC150V_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR3762027.1"
                     /translation="RAALTYPSPTRGYEAIRDAVAFYAGPMDACVVGDERAEPQPGDF
                     YGGWITSGIEGPFKGGPGSAGW"
BASE COUNT         1122 a         2473 c         2486 g         1061 t
ORIGIN      
        1 cttcccgccg ccggactgcg accccccgga ctgcgaagca gggcccgccg acgcacccgc
       61 cgcaatcagc accactgccg ccgcagcgac gagaaacact cgcacgatca ctctcgtgga
      121 cgtcagaccc accccctgcg ggtgcgccca tctttagcga ctctcggcac aagcgtcaaa
      181 taggggctga cacggcctga cacattcacg gatcgggaag gtgagctccg gggttaactc
      241 gctcgcgctc gctccttcgc gagcaacgcc cggacagggt cggtgccagg gctcacgccg
      301 gcggtgcccc tcacgatgca cggcacgcct ctgagcctcc gcgtgtcgcc tgacagggtt
      361 gagccggccg cctgaccggc gcctcacgat ctggcatcag tccggtgtgc cggcgcgcga
      421 actcgacgag agggcaggcc acgcgcggcc ctcgatgagc ctggacgtct acagccacgt
      481 catgcccgcc gacgaggtgc cacatcgagc agcgttcggc ccttatcggc cgccgacatg
      541 ccgtgctggt gtgagcccgg tgtgacctcg ccgccaacaa tcggctgagc ccctctaggt
      601 aagccgaacg agggatttgc gtggggaata cccgcgcggg gggttcccgg ctcgacttcg
      661 ccgaccgatt gctgaagcac cccgaagcgg ggtgcggcaa tggagcggtg tcgtgggcgg
      721 cggcagacgc ggcctcgcgg cgtcgcgtcg agtcaggagg agagaaccag gatgagaaag
      781 ctcatcgtga gcgtcgcgtg cgctctgggt gcgaccgtca tgctctcaca ggcggctctg
      841 gcgcaggatg acgagggaga acgagtctgt cgaggattcg tcgtgcacgg cgtcgtgccg
      901 gaggtcggcg agcgcaccat cgtggtcgcc gcggacagag cacgccccga cggcctcgcc
      961 ggcaccgagg tgagtctgag actcggctca cgcacgagaa tggacggaga ggggccggtc
     1021 gaagtcggca gcagcgtcaa ggcgcgggga accgtgtgcc aggtcgacga ggccgacgag
     1081 cccgtcttct acgcgaagcg cgtcaaggtg aagcgcgttc gggcagagag actccggggc
     1141 gcgttcgagc tggcgggaac cgtcacggac gtctcggaag acgggttcgc ggtcgaggtc
     1201 tcggagtcga gtttcgcgag cctcgtgggg acgattgtgt cgatcgggtt tcggaaccgc
     1261 acaacggtcg aaggcgagct ggccgtcgga gtcgacgtcg tcgtggcagg aagagcacgg
     1321 atccgcggcg gcgagtcggt gctgctcgcg cacgaggtca ccgtcatggc aggcgatgag
     1381 gagccggagc cgccgccgga cgggggcgag taggcagatc gtgcggggtg ggcggccgtg
     1441 cccgcccgcc ccgctcaccg gcgccacaat gcagaggtgc gcataccgga tctgggaagt
     1501 cgcgggcagg gctgggtcta tctgcagttc gtgctcatcg ccgcagtcgt cgttgccggc
     1561 ttcgcgccgc cacgttggcc ggagggtctg gcccgccctt tcgacctcgc cggcgccgtc
     1621 atcgcgctcg gcagcggctt cattgccgtc tgggcggctc gcacgctcgg ccgatcgttg
     1681 actccgtacc ccgctccagc cgacggcggc gtgctcgtcg cggccggccc ctatcgcttc
     1741 gtcaggcatc cggtgtacgc ggccggtatc ggcttcttcc tcggcgtggc gctcgtcacc
     1801 ggcccctggg cgctcgtccc caccgccgtg ctctcggtcg tctgggcgct gaagctccaa
     1861 gtggaggagc ggttcctagg tgagcggttt cccgattacg cgcgctattg cgagcaagtg
     1921 ccggccaggc tcgtcccggg catcttctag ccgctcgact aggctggcgc catgacggcc
     1981 tcgtggcccg aaccggtcga gcgggtcgcc gctcacctgc gtgcctcggg tgcggaggga
     2041 cgcatcgagg agctccccgc cgagactccc acggcgcagg cggcggccga cgcggtcggc
     2101 tgcggtcttg cgcagatcgt gaagtccctc gtcctcgtcg gcgcctcggg tacgtacttc
     2161 atggcgctcg tcccgggcga ccgccgggcc gatctggcga agattgccgc cgccgtgggt
     2221 actacgaagg ttcgcgttgc caggcccgac gaggtcgtcg aggcgacggg cttctcaccc
     2281 ggtgcggtcg cgccgtttcc ggtgcctcga gtcacgcgaa gcgtcgtcga tcaaagcatc
     2341 ctggcgcagc gcgccgtctg gatcggcgcc ggctcgacga ggcatctcgc gtgtctcgcg
     2401 ccggccgagc tcgtgcggct gtcgcgcgcc gacaccgtgg acgtgatcga ggcgcccgaa
     2461 taccattccc agtccgagtc ggagaccaag gagtcgtaga tgcaggcaac cgaaaagatc
     2521 tggatgaacg gcgagctcgt ggactgggac gacgcgaaga tccacgtcgg cgttcacggc
     2581 cttcactacg gcaccggcgt gttcgagggc atccgctgct acgagacgcc gaagggcccg
     2641 gcagtgttcc ggctggccga gcacatgcag cgtctgcacg actccgcgcg cctgctgtac
     2701 atgggaattc ccttctccgt ggaggagctc cgccaggcga cgcacgatct cgtccgcgtc
     2761 aacggcctgc cttcttccta catccggccg atcgccttct acgggtacgg agagctcggc
     2821 gtccacacgg gcggaaaccc ggtcgagacg gtgatcatga actggccctg gggcgcgtac
     2881 ctgggagagg acagcctcca gaaggggatc accgcgaaga tctcgagctg gacgagagtg
     2941 ggcccgaacg tcatcccgca tgtcgcgaag gcaaccggca tctacctcaa ctccatgctc
     3001 gctacctccg aggccctgcg tgccggctac aacgaggcga tcctgctcac ggatcacggg
     3061 acggttgcgg acggcccggg cgagaacatc ttcgtcgtca aggacggcgt gattttcacg
     3121 ccgccgctct cgacctcgat cctgccgggc atcacgcgcg actcggtgat gacgatcgcc
     3181 cgcgagcagg gctacgtcct cgaggaacgg gacatcatcc gcaccgacct ctatctcgcc
     3241 gacgaggtgt tcatgacggg gacggccgct gaagtcacgc cggtccgagc cgtcgacgac
     3301 gtcgagatcg gcgttggacc cgtgacgctc gccctgcaga agacgtacct ggacatggtc
     3361 aacggggtcg acgagccgtg gggcgactgg ctcgacgtcg tcgagacgcc ggcgcgcgcc
     3421 gaggcgtgag cgcggcgacg cgccagatcc cgctctccgc tccctggctc gacgaccgcg
     3481 aggaggagct cgtcctcgac gtgctccgct cggggcgact ctcgctcggg cccgcgatcg
     3541 agcgtttcga ggagctgttc gcccagacgg tcggtgcacc gtatgccgcc gccgtctcga
     3601 gcggaacggc cggcctgcac ctgctctgtc gcagcgccgg tctcgcgccc ggcgacgaag
     3661 ccatcacctc cccctactcg ttcgtcgcat cggccaactg cgcgatctac gagggcgcga
     3721 cgcccgtctt cgccgacatc gaccccggaa cgctgaatct cgaccccgcc acggtcgagg
     3781 cggtggtcac cgagcgcacg agagcgatca tcgcggtgga catcttcggc tacccctgcg
     3841 agctggagcc cttgcgcgag atctgcgacc gctacgggct cgtcctgatc caggacgcgt
     3901 gcgaggcgct cggggcgcag tacagaggag cgcccgtggg ctcgcacggg ccgtcgtcgg
     3961 tcttcgcctt ctatcccaac aagcagatca cgacgggcga gggcggaatc gtcgcgacgc
     4021 actccgagga cgaatggcgc ctgctgaaga gccttcgcaa ccagggacgg gccgacggcg
     4081 gcggctggct cgaccacacg ctcctcggtt tcaactaccg aatcgacgac atccgcgccg
     4141 cgatcgggat cggccagctc gagcggctcg accgcattct cgccgcgcgt gccaaggttg
     4201 ctacgcgcta cggtgacctg ctctccgcga ttccgggcct cgagctgcct cttgcggacg
     4261 acgcggaaca cgtgcgatcg tggttcgtct acgtcgtgac gcttccgccc gaggtgcaac
     4321 gggagcaggt catcgccgcg ctgaccgagc ggggcgtcca gaccgcccgc tatctcccgt
     4381 gcatccatct gcagagctac atgcgcgagc ggtacgggtt tgcggagggt ctctgcccgg
     4441 tggccgaggg tctgagcagc cgcacgttgg cgcttccctt ccatgcccgc ctctccccgg
     4501 acgaccagga ctacgtcgtc gaggcgctcg aggcgtcgct cgagctcgtg gcggccgcgg
     4561 gatcgtccgc cagccggtag cggcggaaga cgaggaagcc gccgaagacg atcgccccgg
     4621 cgacgaggcc ggcaacgcgc actccggcct ggctctcggt cgtgcgtccg agaatcaaca
     4681 gcaggcccag caagagcggc gtcagggagg tgaccgtctt ctcgacgagg ttctgcgccc
     4741 cgaagtaggt cgcctcccgc ctgagccccg agcgggtctg gtcatcgtcg aggatgtccg
     4801 ccgtcagggc cgcaggaaac aggaaagcgc ccgcaatggg cacgccgacg gccgcgatca
     4861 gaacgacgag ctgcacctcg gcgggcacga acggcacgat cccgaccagc ccgagcagcg
     4921 ggaacagaag cgaggctccc agcatcgagg cgagatacac gcgacgcttg gaggtacgcc
     4981 gtgcggcgcg ggtcaggaac ggcaccgaga cgaggacgac ggcgatcgcc acggccgtca
     5041 gaccggcgac ccagcgatcc tcttcttcca cgtcgagcac cgccttgacg aagaacggaa
     5101 ggtcggcgag caggagttgc acacccacct gaaagagcac gaaagagggg agaaagagca
     5161 gaaacgagcg gttcgccacg gtgaggcgaa gagcctggcg cagcgagatc agcgcgggcg
     5221 gcgtcgtcct gctcgcccgc cgccaagcac cggcggtgcc caggtagcgg aagccgacgg
     5281 cgatcacagc cattaccgtc gccatcgccg caaagccgac gctgtcgatc aggagcgagg
     5341 ccccgaccag accgatcgcg ccaccggcgg cgccgaagta cacgcgggta ccgacgaccg
     5401 ctaggcggtc ttccgtcgtc tgggcgatct cggggaagag cgcctcgtac gggcccgacg
     5461 agagcgtcga gaagaggaag tagccgaaga gcgtgacggc gagccagaga gcggccccgg
     5521 ttccgtcggc gggcggcatg aagagcagcg cggcgaagag cccgacgaac ggggtggcca
     5581 gcacgatgaa cggcagccgc ctgcccaggc gcgactctgt ccggtcgctc caccaaccga
     5641 cgacaacgtc gtcgagcgac tcggcgagcc gcaccagcgt gaggatgact ccgaccgcca
     5701 aaggactcag cagctcgggg agatctgcgt cctccggtgg cgcgtagtag tagacgagcc
     5761 agaggttccg gctctgcgtg agcgcctcgc cgcccaggct ggccgacgcg tacagaatcc
     5821 tcgtgtggcg ggggagcggg gttgtgaccg ccgtcatcag cgtattctcc gagccatcga
     5881 tcgcagccgc ctcagtgctt caccacgatt gcgcgcggcc ttgattctgg gcgtcgtcct
     5941 ggacaggccg gtcgtcggct ccgccctccg cggtctcacg ggcactccag ccatctccga
     6001 ggtgcgtgcc gatgacgtgc cggcaaccct cgcggtgccg gcgggccgtg gcccctggcc
     6061 cgcggcggtc ttcgtcaacg gagtgaccgc tcgcgggcgt gggcaccccc aggtcaggcg
     6121 gctggccgag ggactcgcgc gagcgggtgt cctcgcgctc gtgcccgagc ccgcggggct
     6181 cgcaaccggt gagctgtcgc cgacgacgat cgagcagacg gtcgcggcat cgctggccgt
     6241 cgccgaccgc ccagacgccc tggccggctc ggtggctctc ttcggagcat cggccggcac
     6301 cgctctcgcg ctgatcgcgg cgcagcagga cacgctccgc gagcgtgtat ccgtcgttgc
     6361 gggcaccgtg ccgtacgcgc acctgcgcga ggcggcccgg gttgccacca cggccacgta
     6421 cgcgctcgac ggtcgccggc ggccgtggca ggcgccgcgg ttcctcggcc tcgtgatcgc
     6481 tcgctcgatt gtggccgcgc ttcgcgcgag ccagacgagg gaccgcctgc gagacgcgct
     6541 gctcgcgctg cccgacgaca cagcagaccc gttcgcgccg ttccgcgagt ggcccaaagc
     6601 gccccacgcg cgggccgtcg cagcgttgct cacgaacgag gatccggttc gattcgagac
     6661 gctctacgac gatcttcccg agtacctgcg agacggtctc gaccgcttgt cgccgctccc
     6721 aggtgcttca gcggtcgccg cgccggtcga gctcgtctcg gcggagcagg actcgtactt
     6781 tccgctcgcc gacggccgtg cgctcgcggc ggcaactggc ggccggctca ctgtgacgcg
     6841 ggcgctccgt cacgccgttc cgaagccgac accgagcggg ctcgcagatg tcggccgcct
     6901 ctacgactgg gcgacgcggt cgctcgccgc cgccggtcgc gtcaccagcc cgccgagccc
     6961 ggtccgccct tgaacggccc ttcgatcccg ctcgtgatcc agccgccgta gaagtcaccg
     7021 ggttgcggct cggcgcgctc gtcgccgacg acgcacgcgt ccatcgggcc cgcatagaaa
     7081 gcgactgcgt ccctgatcgc ctcgtaaccg cgggtgggtg acgggtacgt caaggcggcg
     7141 cg
//
LOCUS       DBNGNK010000122         2891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_951632,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000122 DBNGNK010000000
VERSION     DBNGNK010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_951632"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC150V_03560"
     CDS             <1..389
                     /locus_tag="AC150V_03560"
                     /inference="COORDINATES: protein motif:HMM:NF019122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR3762028.1"
                     /translation="GLGLGDNAKSGLVTRGLAEMRRLADAAGARPETFAGLAGMGDLI
                     VTCWSPSGRNRRAGELIARGASPDEAAAEIGMVVEGLTTAPVLRELSRRLDIELPITE
                     GVCEVLAGEKLTDLLEQLMRRAPTTE"
     gene            421..1665
                     /gene="metK"
                     /locus_tag="AC150V_03565"
     CDS             421..1665
                     /gene="metK"
                     /locus_tag="AC150V_03565"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR3762029.1"
                     /translation="MREFKFSSESVTEGHPDKIADQISDSVLDAVMAQDPGGRVAIET
                     LVTTGLVVVAGELTTETYVDIPRLVRERISEIGYTRAKYGFDAETCGVIVAIDPQSPD
                     IAQGVDESYEVQHGDDDPIDQTGAGDQGMMFGYASNETDELMPLPIMLAHKLTRRLSE
                     VRKADVLPYLRPDGKAQVTVRYEVDEHGRQRPVEIEQVLVSTQHRDGLDVDSLIRQDL
                     VEHVVEPVLHVDYGELCDPRRIHEKDFFYVNPTGKFVVGGPMGDTGVTGRKIVVDTYG
                     GAAPHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAGLAERCLVQVAYAIGVAHPLSL
                     LVDCYGTETRPLSEIEALITEHFDLRPGAIIEQLDLRRPIYTKTAAYGHFGRDDRDFT
                     WERTDKADGLRRAAGLEAASPA"
     gene            complement(2190..2753)
                     /locus_tag="AC150V_03570"
     CDS             complement(2190..2753)
                     /locus_tag="AC150V_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762030.1"
                     /translation="MLVVGAGAAYAAKGGNDGLKRTAQKLAAKSQFKADLANRLGTTV
                     EKLDAAVKAAALARINAAQQSGDITADEAAALKSAVENGRLAKRIALPADVAKQLGTT
                     EEKLDEARTEARKAQMKSRVDQAVADGKITKEYGDELKQRIDEADFSAGGKRGFGKRG
                     FGPGFGKFGGPPRSGSSEGAKPAALVF"
BASE COUNT          443 a          965 c         1020 g          463 t
ORIGIN      
        1 acgggctcgg cctcggtgac aacgcgaaat cgggtctcgt cacgcgcggg cttgccgaga
       61 tgcgaaggct ggccgacgcc gcaggcgccc gcccggagac gttcgccggg ctggccggga
      121 tgggcgatct gatcgtgacc tgctggtcgc cgtcggggcg aaaccggcgc gccggcgagc
      181 tgatcgcgcg cggcgcctca cctgacgagg cggccgcgga gatcggcatg gtcgtcgagg
      241 ggctgacgac cgcgccggtg ctgcgggagc tgtcgagacg actcgacatc gagcttccga
      301 tcaccgaggg cgtctgtgag gtgctcgcgg gcgagaagct gaccgatctc ctggagcagc
      361 tcatgcgccg ggcgccgacg accgagtagg tgactggaac gtcaggtagc ctcctgcccc
      421 atgcgtgagt tcaagttcag ctccgagtcc gtcaccgagg gccatccgga caagatcgcc
      481 gaccagatct cggacagcgt gctcgacgcg gtcatggcac aggatcccgg cggcagggtc
      541 gcgatcgaga cgctggtgac gacggggctg gtcgtcgtgg ccggcgagtt gacgacggag
      601 acgtacgtcg acatcccgcg gctcgtgcgg gagcgcatct cggagatcgg ctacacgcgc
      661 gccaagtacg gcttcgatgc ggagacgtgc ggcgtcatcg tcgcgatcga cccgcagtcg
      721 cccgacatcg cgcagggggt ggacgagtcc tacgaagtgc agcacggcga cgacgacccg
      781 atcgaccaga cgggagcggg tgaccagggg atgatgttcg gctacgcgtc gaacgagacc
      841 gacgagctga tgccgctccc gatcatgctc gcccacaagc tgacgcggcg cctctcagag
      901 gtccgcaaag cggacgtgct gccctacctc cggccggacg gaaaggccca ggtgacggtt
      961 cgctacgagg tcgacgagca tgggcgccaa cggcccgtcg agatcgagca agtgctcgtc
     1021 tcgacgcagc accgcgacgg gctcgacgtc gactcgttga tccggcaaga cctcgtggag
     1081 catgtcgtgg agccggtgct gcacgtcgac tacggcgagc tctgcgatcc tcgtcgcatc
     1141 cacgagaagg atttcttcta cgtcaacccg accggcaagt tcgtcgtcgg cggcccgatg
     1201 ggggatacgg gcgtgacggg ccggaagatc gtcgtcgaca cctacggcgg cgccgcaccg
     1261 cacggcggcg gggcgttcag cggcaaggat ccgaccaagg tcgaccgctc cgcctcctac
     1321 gcggcgcggt acgtcgcaaa gaacatcgtc gccgccggcc ttgccgagcg gtgcctcgtc
     1381 caggtcgcct acgcgatcgg ggtcgcgcat ccgctttcgc tgctcgtcga ctgctacgga
     1441 accgagacca ggccgctcag cgagatcgag gcgctgatca cggagcattt cgatctgcgc
     1501 cccggcgcca tcatcgagca gctcgacctc cgccgcccga tctacaccaa gacggccgcg
     1561 tacggacact tcgggcgcga cgatcgcgat ttcacctggg agcgcaccga caaggcggac
     1621 gggctccggc gtgcggccgg gctcgaagcc gcatctcccg cgtagcgctc ggatcgaccc
     1681 tccggcatcg gtaccgcgag gcgctcgtcg agcacacgct cgattcgctc cctcgtgtcg
     1741 gtcacgatgt cgtcgcgcgc ggcgtcggag agctggccgg cctcgaccgc gcgagcgagc
     1801 cgtgctcgcg tgacgtcgac catgaccccg acgagcagag gtctcgagac gccccgttgg
     1861 gcggcgaggt cggcgagcct gactccacgt gacagctcct cggcgagctc agcccggccc
     1921 aggcgaaggc agccggccgc ggccgcgagc tccggtcgca tccgggcggg tcggatgttc
     1981 ttcggtcggt tggggagtgt ggcgtccacg ggcagctcct ttcgtcggtc ggctggggct
     2041 tgcacttccc attggagcgg tgcgacctga gacggaggtg agaagggcct cttaatcagc
     2101 tgagacgtcc gtccggggcg tcggctgaac gcgaaggccc gccaagagcg ggccttcgcg
     2161 ctgctcgggg agggacgagc ggaggagagc tagaagacga gcgcggccgg cttggcgccc
     2221 tcggacgatc cgctcctggg aggcccgccg aacttgccga agccgggccc gaagccgcgc
     2281 ttgccgaagc cgcgcttgcc accggcggag aagtcggctt catcgatccg ctgcttcagc
     2341 tcgtcgccgt attccttcgt gatcttgccg tcggcgaccg cctggtcgac gcgggacttc
     2401 atttgcgcct tcctggcctc cgtccgggcc tcgtcgagct tctcttcggt cgtgccgagt
     2461 tgcttcgcga cgtcggcggg aagcgcaatc cgcttcgcca ggcgaccgtt ctcgacggcg
     2521 ctcttcaggg cggcggcctc gtcggcggtg atatcgccgg actgctgggc ggcgttgatc
     2581 cgcgccagcg ccgccgcctt cacggcggcg tcgagcttct ccaccgtcgt tccgagtcgg
     2641 ttggccaaat cggccttgaa ctgcgatttg gcggccagct tctgcgcggt gcgcttcagg
     2701 ccgtcgttgc cgcctttggc cgcgtacgcg gcaccggcgc cgaccacgag cacgagcgcc
     2761 gtgacgacgg cgatagcggc ggtgcgggtg atccctctca tcgagtctcc ttctaggtcg
     2821 gatgatcgga cgctctcgat cgaatcagcg caacctgaga ggagcctgag acgcctgtaa
     2881 gtgtttcttg c
//
LOCUS       DBNGNK010000123         3228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_93047,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000123 DBNGNK010000000
VERSION     DBNGNK010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_93047"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2941)
                     /locus_tag="AC150V_03575"
     CDS             complement(<1..2941)
                     /locus_tag="AC150V_03575"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HIR3762031.1"
                     /translation="MHDSRTRGLNSAYVQQLREDYLEAPASVPAEWRALFERGDDALV
                     EEHFETQPAPPLDGGGNGRAAIEPAPAASGSARSVAPPTEASALRPATAPVPHPPAAP
                     LPARPAALELVDEHAPAPPVRPSAVEPSEELLGAVAAATALVKALRMHGHLAARLDPL
                     GSEPMGDPAIEETTLVPPLRTELKERIPASLLRLYVEGDTLADVLPALREVYTGSIGY
                     QIEHISDHAERVWLRRAIESGQFRRPLPADERLELLRSLSRVEGFEQFLRRSFLGQKQ
                     FSIEGLDALVPMLEESIQIAAADGAHGVVIGMAHRGRLNALTHLVGRPYESILREFEG
                     ERTLDALVDDPEGGTGDVKYHLPAAGTRATKSGEIEISIVPNPSHLEAVDPVVVGRAR
                     AEQTDRSSGAGRHDPDVALPILIHGDASFAGQGVVAETLNFQSLEGYGVGGTLHIIAN
                     NQVGFTTDPEESRSTRYSSDLAKGFDVPIVHVNADDPEAALSAVRLALAYRREFGHDF
                     VIDLVGYRRFGHNEQDEASYTQPLMVSRIEQHPPVRASYAATLVEDGLLTAAEAEAML
                     DDTVAELRAAHERLKASFGGHPPAERRDQSRSTTGDVVETSVPAERLDQLNDALLAVP
                     DDFQLNPKLERQLERRRDADGEGGVDWGQAEALAFGSLLEDGIPIRLSGQDTERGTFS
                     HRHLVLHDPNTGETYAPIQHLPGARASFEIYNSPLSEFGALAFEYGYSVAAPEALVLW
                     EAQFGDFVNAAQVVIDQFIVAGRSKWGQTSRLTLLLPHGYEGNGPEHSSARLERFLQL
                     AAQDNIRIANCTTAAQYFHLLRRQALDATARPLVVMTPKGLLRLRQAASGLDELSGGA
                     FLPVIDDPVADRDAARRLVLCSGKIYYDIQNHDDREKATEVAVARLEQLYPFPVEAVT
                     SLVASYPRLEEMVWVQEEPQNMGVWRSLRHRLEDAARVARDRERRGTACGRLHGRLEV
                     LHPVRVGA"
     gene            complement(3004..>3228)
                     /locus_tag="AC150V_03580"
     CDS             complement(3004..>3228)
                     /locus_tag="AC150V_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762032.1"
                     /translation="DAVAGFLAAASLALSLVVLAERPARLGPVAILLALLAARMSTRW
                     ERLSLFATAAAMVAFTAGMAFAVLTGNPIF"
BASE COUNT          504 a         1076 c         1156 g          492 t
ORIGIN      
        1 gtgcgcccac gcggaccggg tgcagaacct cgagccggcc gtgcagacgg ccgcacgccg
       61 tgccgcggcg ctcgcgatcc ctcgcgacgc gcgccgcgtc ctcgaggcgg tggcgcaacg
      121 agcgccagac ccccatgttc tgcggctctt cctgcaccca taccatctct tcgagacgcg
      181 ggtaggaagc gacgagcgac gtaacggcct cgacggggaa ggggtacagc tgctcgaggc
      241 gcgcgaccgc gacctcggtg gccttctccc gatcgtcatg gttctggatg tcgtagtaga
      301 tctttcccga gcagaggact aggcggcggg ccgcatcccg gtcggcgacc ggatcgtcga
      361 tgaccggcag gaacgccccg ccgctcagct cgtcgagccc ggaggcagcc tggcgcagtc
      421 ggagcagccc cttcggagtc atgaccacga gcgggcgagc ggtcgcgtcg agcgcttgtc
      481 ggcgcaggag atggaagtac tgcgcagccg tcgtgcagtt ggcgatccga atgttgtcct
      541 gtgcggcgag ctgcaggaag cgctcgagtc gcgcgctcga gtgctcggga ccattgccct
      601 catacccgtg cggcaggagc aacgtaagac gagaggtctg tccccacttc gagcgcccgg
      661 caacgatgaa ctggtcgatc acgacctggg cggcgttgac gaagtcgccg aactgagcct
      721 cccaaagcac gagcgcctcc ggagccgcca ccgagtagcc gtactcgaac gccagcgccc
      781 cgaactccga gagcggtgag ttgtagatct cgaacgatgc ccgcgctcca gggagatgct
      841 ggatcggcgc gtatgtctct cccgtgttcg ggtcgtgcag cacgagatgc cgatgcgaga
      901 acgtgcctcg ctccgtgtcc tggcccgaca ggcggatggg aatcccgtcc tcgagcaacg
      961 atccgaacgc gagcgcctcc gcctggcccc agtccacgcc tccttcgccg tcggcgtcac
     1021 gccggcgttc gagctgtcgc tcgagcttcg ggttcagctg aaagtcgtcg ggaaccgcga
     1081 ggagcgcgtc gttcagctgg tcgagccgct cggcgggaac gctcgtttcg actacgtcgc
     1141 cggtcgtcga gcgcgactgg tcgcgccgct ccgccggggg atgcccgccg aacgaagctt
     1201 tgagccgctc gtgcgccgcc cgcagctcgg cgacggtgtc gtctagcatc gcctcagctt
     1261 cggccgcggt gaggagaccg tcctcgacga gcgtcgccgc atacgaggcc cggacgggtg
     1321 gatgttgctc gatgcgcgag accatgagcg gctgcgtgta ggaggcctcg tcctgctcgt
     1381 tgtgaccgaa gcgccggtag cccaccaggt cgatcacgaa gtcgtggccg aactcacgcc
     1441 ggtacgcgag ggccaagcgg acggcgctga gggctgcttc gggatcgtct gcgttgacgt
     1501 gcacgatcgg cacgtcgaac cccttcgcaa ggtcgctcga gtacctcgtc gagcggctct
     1561 cttccggatc ggtggtgaag cccacctggt tgttcgcgat gatgtggagc gtgccgccga
     1621 cgccgtagcc ctcgaggctc tggaagttca gcgtctcggc gaccacgccc tgtccggcga
     1681 acgaggcgtc gccgtgaatg aggatcggca gcgcgacgtc aggatcgtgt cggccggccc
     1741 cggacgaccg gtcggtctgc tccgcccgag cgcgtccgac gacaaccggg tcgacggcct
     1801 cgagatggct cggattcggc acgatcgaga tctcgatctc accggacttc gttgctctcg
     1861 tgccggctgc cggcaggtgg tacttgacgt ccccggttcc gccctccgga tcgtcgacca
     1921 acgcgtcgag tgtccgctca ccctcgaact cgcgcaagat cgactcgtac gggcgaccga
     1981 cgaggtgcgt gagcgcgttg agtcggccgc ggtgagccat accgatgacg acgccgtggg
     2041 cgccgtcggc ggccgcaatc tggatggact cttcgagcat gggcacgagc gcgtcgagac
     2101 cctcgatgga aaactgcttc tgcccgagaa acgatcgccg caagaactgc tcgaagccct
     2161 cgacgcgcga gaggctgcga agaagctcga gcctctcgtc ggccggcagc ggccggcgaa
     2221 actgcccgga ctcgatggct cgccgcagcc agacacgctc cgcgtggtcg gagatgtgct
     2281 cgatctggta gccgatcgat ccggtgtaga cctcgcgcag cgcgggcagc acgtccgcca
     2341 gggtgtctcc ctcgacatag aggcgaagga gcgaggctgg aatccgttcc ttgagctcgg
     2401 tgcgcagcgg cgggacgagg gtggtctcct cgatcgcagg gtcgcccatc ggctcggagc
     2461 cgagcggatc caggcgggca gcaaggtggc cgtgcatccg cagcgccttc accagtgccg
     2521 tcgccgcggc gaccgcgccg aggagctcct cgctcggctc gaccgcgctc ggacgcacgg
     2581 ggggagccgg ggcatgctca tcgaccagct cgagggccgc gggacgtgcg gggagcggag
     2641 ctgccggtgg gtgcggaacg ggggcagtgg ccgggcgcag cgcagaggct tcggtgggcg
     2701 gggcgacgga acgcgcggag ccactcgccg caggggccgg ttcgatcgcg gctcggccgt
     2761 tgcccccccc gtcgagcggc ggcgccggct gggtctcgaa gtgctcctcg acgagcgcgt
     2821 cgtccccgcg ctcgaacagc gcccgccatt cggcggggac gctcgcaggc gcctcgaggt
     2881 agtcctcgcg caactgctgg acgtaggcgg agttcaggcc gcgcgttcgg gagtcgtgca
     2941 tgcctccctg tgttgtagca ggggctccgc ccggagcaac gggtgagtcg gcgatctcgg
     3001 gagctagaag atgggattgc ccgtcaggac ggcaaaggcc attcctgccg tgaacgccac
     3061 catcgcggcc gccgtcgcga acaggctcag cctctcccag cgcgtgctca tgcgagccgc
     3121 gaggagggcg aggaggatcg cgaccggacc gagtcgcgcg ggacgctcgg ccagcacaac
     3181 gagcgagagc gcgagggacg cggctgcgag gaagcctgcg acagcgtc
//
LOCUS       DBNGNK010000124         8131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1024344,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000124 DBNGNK010000000
VERSION     DBNGNK010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1024344"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /gene="asnB"
                     /locus_tag="AC150V_03585"
     CDS             <1..1570
                     /gene="asnB"
                     /locus_tag="AC150V_03585"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR3762033.1"
                     /translation="LGLGFAERLRGMFAIAVWDSRRRRLVLARDRFGIKPLAYRVDGD
                     SLEFASELRALGPGDIDLDALEGFLAFNSVPGPSTIFRNVRKLPAGHLLVWEDGGVRF
                     ERFARPAPAAAGDVRTEDERELAIELRERLADSVRAHLVSDVPVAVFLSGGIDSSLLT
                     ALAAASSSESLRTYSIGFAESTFDETPDARLVAQRFATRHEELVLRPDAALLLPALAD
                     AFDEPFADSSALPTYLVADLAARDVKVALSGEGGDELFAGYFTYAADLLAPRLSAAAR
                     LVRPLVERLPVSTARTSFDYKAKRFVRAAHLPPLERHLGWKEIFSTDARAELTGTRSD
                     FDPVETARGRYEETAGAEQLARLQDVDLGTYLVDDLLVKTDRATMAHSLEARVPFLDT
                     SVTELALALPTRMKVSGLRKKVLLRKAAEPLLPRAIVRGRKRGFSIPAAAWLRGELEP
                     FARDVLSADTLGRHGFFRPEPVRRLIDAHVGGREDLSRQLWCLLSFTLWYERHVERPP
                     GPVRGARIAELIAA"
     gene            1689..2687
                     /locus_tag="AC150V_03590"
     CDS             1689..2687
                     /locus_tag="AC150V_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF354 domain-containing protein"
                     /protein_id="HIR3762034.1"
                     /translation="MKVWIDMTASAHVLVFRPLIRLLQEEGHDVEVTAREYAQTLQLL
                     ELHRIPATVVGRHGGRSRLGKARSLASRLELLRRWARGRRFDIALAHGSHELTITARR
                     LGIPSATTFDYEWATLQHQLGCRAATRVVTPDAIPIARLARFGAVPPKLRQYPGLKEE
                     YYLADFEPDSKALRSLGVDEGRILVVLRPPPDVSLYHRHSNPLFPQVLDRLGNDPKVQ
                     AVVIPRTDEQRWTIAALTLPSMILPERAVDAQSLIGLADLVVSAGGTMNREAVALGTP
                     VYTTYGGRLGAVDEQLIREDRLRPLAAAHDIALEKRSSTAERVRRDPRLMLDLLLS"
     gene            2753..3868
                     /locus_tag="AC150V_03595"
     CDS             2753..3868
                     /locus_tag="AC150V_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3762035.1"
                     /translation="MGDSITLGRFFGIPIGINWSWLIVFGLITWTLADSVFPDQNPGL
                     SSGSYWTMAVVAAALFFVSILLHELGHALQAQRDNVKIEGITLWLFGGVARFSGRIPS
                     AGAEFRITIAGPLVSLALGLLFSGVALLIGLPSAADGVAAWLGYINLVLLVFNLLPAF
                     PLDGGRVLRSAIWARNGDLARATRIAAAVGRVIAVGLIVLAVLMLVIGNVIGGIWLGF
                     IGWFLLQAAGAESQQATVTERLGELTVRDLMTEAPVTVPSQLTLDRFVDELVWTARFT
                     TYPVVDNGDVVGLLPFRCVAEVPRSEWPRRTVGSCMLALRDVPQFGPDTTASDALPDL
                     ASGTVGRALVLDGGRLVGLLSASDLARALELDGSGRR"
     gene            complement(3873..5771)
                     /locus_tag="AC150V_03600"
     CDS             complement(3873..5771)
                     /locus_tag="AC150V_03600"
                     /inference="COORDINATES: protein motif:HMM:NF014740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR3762036.1"
                     /translation="MRLRFPFSRHSLWQVAVDGAIVVLAWWLAWKLRFDQGRPVYYDR
                     YMEWGILLVIVGIKLPVFALSGFYNRWWRYVSTRDMWSILRGCALASVAVFLVFTFLE
                     LHRVRVPSGVWFIDLLLCIALIAGSRLAARTLLERPRPGSIVARGKEAIVIGAGDAAQ
                     LVVKEMLRNPALGYTPIALVDDDPRKRNLRLHGIRVLGTIDELSRIIHERRPDEVLIA
                     IPSASGELRERIVEIARVEDIPVKTLPGLNELISGDYNLAGQLRPVEVEDVLGREPVE
                     VDLDAIAGYLTGEVVLVTGAGGSIGSELCRQIARVGPTRLVLVDNSEPALFEIERELV
                     DERGFHAAAAVLGDVGNEAKMRQVFDKYRPGVVFHAAAYKHVPLMEANPLEAVRNNTI
                     GTKTIAHVAIDADVKRFVLVSTDKAVNAKTVMGQTKALCEWIVEAYGAREEVASRFVA
                     VRFGNVLGSSGSVIPIFRRQIANGGPLTVTHREMTRFFMTIPEAVQLVIQAGAIGESG
                     HVYVLDMGEPVSIVDLAEKMIRLSGKEPGVDISIEFIGARPGEKLHEELVGDGENVSP
                     SSHEAIMLVTRPALDAEWLDAELERLQQLVEEGDTLEAVGALNRIVRESRRIPDPGRT
                     RAAGSVEL"
     gene            complement(5768..6850)
                     /locus_tag="AC150V_03605"
     CDS             complement(5768..6850)
                     /locus_tag="AC150V_03605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3762037.1"
                     /translation="MIPLVDVKAQYAPLIPDLKERFAAVLDSGRFIFGPEVEAFEREA
                     AAYLGVPHAIGVANGTDALVLALEAMGVGAGDEVICPAFTFYATAEAIARTRAIPVFA
                     DIDPVTLNLDPAAVADRITPRTKAVVPVHLFGRPAPNAELAAFGVPLLEDAAQAFGAP
                     GVAQAGVCSTFSFFPTKNLFALGDGGLVASNDDDVAATVRKLRFHGSADKQTFELIGA
                     NSRLDAIHAAALRVFLPRLSGWNAARREAASIYAELGLGEAVELPVDEPAHVYHMYVV
                     RSTERDRIASRLREAGIASAAYYVVPMHLQPAMRYLGWEPGSLPETERAAGENLALPM
                     WGGIGREIQERVVDAVLSAVGVVTAP"
     gene            complement(6850..7554)
                     /locus_tag="AC150V_03610"
     CDS             complement(6850..7554)
                     /locus_tag="AC150V_03610"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3762038.1"
                     /translation="MPVASILTDISHWITGTIGDYGLYAIFLLMLVDAVLPAASELIM
                     VYAGALAAGAFTSQTVTLFGWELSEGFGAFVAVVLAGTIGYTIGSILGWAIGDYAGRP
                     YLERHGRWLHLDNAKLERADRWFERWEDWAVFLGRLTPVVRSFISVPAGVFRAPLGRY
                     TLLTFAGSTIWCLLLGGIGFALGSNWERFHDAFRYVDYVVIAAVVVVIALVGWRLLRR
                     RKAGNEPAPGYTPGSD"
     gene            complement(7622..>8131)
                     /locus_tag="AC150V_03615"
     CDS             complement(7622..>8131)
                     /locus_tag="AC150V_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR3762039.1"
                     /translation="AGATHAPLSTSTSRAPQLYPDVGSLKEGKWRVTVRAVDEDGQVS
                     KMARSFKLNTTLGFVRTSPRRLFLPPEGRELEIRWRLGRPARVRVWIESTNGEIVRSF
                     TMRRYSKGLRRVIWDGLDRKGKPVKGGLYRVHVLARNALGNVELERKLGVQRIKGPKR
                     ALDTGVDDA"
BASE COUNT         1253 a         2871 c         2726 g         1281 t
ORIGIN      
        1 gctcgggctc ggctttgccg agcggctccg ggggatgttc gcgatcgccg tgtgggactc
       61 ccggcgccgc cgactggtgc tcgctcgcga tcgttttggg atcaagcccc tcgcctaccg
      121 cgtcgacggc gactcgctcg agttcgcctc ggagctccgc gccctcgggc cgggcgacat
      181 cgatctcgat gcgctcgagg gattcctcgc cttcaactcg gtgcccgggc cgagcacgat
      241 ctttcgtaac gtccgcaagc ttcccgccgg gcatctcctg gtctgggagg acggcggtgt
      301 caggttcgag cggttcgcac gcccggcacc cgcagccgcc ggcgacgtcc gcaccgagga
      361 cgagcgtgag ctggcaatcg agctgcgcga gcggctcgca gactccgtgc gggcacatct
      421 cgtttccgac gtgcccgtcg cggtcttcct ctccgggggc atcgactcgt cgcttctgac
      481 cgcgctggcc gcagcgtcga gcagcgagtc gctgcgcacg tactccatcg gctttgccga
      541 gagcacattc gacgagacgc ccgacgcccg cctcgtcgcg cagcgcttcg cgactcgaca
      601 cgaggagctc gttctccgtc ccgacgccgc gctgctactg ccggcgcttg ccgacgcgtt
      661 cgacgagccg ttcgctgatt catcagcgct cccgacatat ctcgtcgccg accttgccgc
      721 gcgcgacgtc aaggtcgcac tgtcgggtga aggaggcgac gagctgttcg ccggttactt
      781 cacctacgcc gccgacctgc tggcaccacg tctctccgcg gccgcgcggc tcgtgcgccc
      841 gctcgtcgag cgcctgccgg tgtcgacggc gcgaacgagc ttcgactaca aggcgaagcg
      901 cttcgtgcgc gcggcgcacc tgccgccgct cgagcgccat ctcggctgga aggagatctt
      961 ctcgaccgac gcgcgcgccg agctcaccgg cacgcgctcc gacttcgacc cggtcgagac
     1021 ggctcgcggt cgctacgagg agacggccgg cgccgagcag cttgcgcgcc tgcaggatgt
     1081 ggatctcggt acgtacctgg tcgacgacct gctcgtgaag accgaccggg cgacgatggc
     1141 gcactcgcta gaggcccgtg tcccgttcct cgacacgagc gtgaccgagc tggcgctggc
     1201 gctgcccact cggatgaaag tcagcggctt gcggaagaag gtgctgcttc ggaaggcggc
     1261 ggagccgctc ctgccacgcg cgatcgtcag gggacgcaaa cgcggcttct cgatcccggc
     1321 ggcggcctgg ctccgcggcg aactcgagcc cttcgcgcgc gacgtgctct ccgcggacac
     1381 gctgggccgc cacggcttct tccgccccga gccggtgcgg aggctgatcg acgctcacgt
     1441 cggcggccgc gaggacctga gcaggcagct gtggtgcctg ctctcgttca cgctgtggta
     1501 cgagcggcac gtcgagcggc cgccgggccc ggtgcgaggc gcccggatcg cggagctaat
     1561 cgcggcgtga ccggatccgg ctcggcatgc tcggatcgcc gccagcgagc ggcgacaggc
     1621 aacgcaagta acagactgtt actagcgttc atcccggcgc gtcgctcgcg cgtcggcgag
     1681 gagcgcgtgt gaaggtctgg atcgacatga cggcgtcggc gcacgtactc gtgttccggc
     1741 cgctcatccg tctgttgcag gaggagggac acgacgtcga ggtgacggcc cgtgaatacg
     1801 cgcagactct gcagttgctc gagctgcacc ggattccggc gaccgtcgtc ggccgccacg
     1861 gagggcgctc gcgcctgggc aaggcacgct cgctcgcgtc acgcctcgag ctcctgcgcc
     1921 gctgggcgcg cgggcgacgg ttcgacatcg cgctcgcgca cggctcccac gagctcacga
     1981 tcacggctcg ccgcctcggc atcccatcag cgacgacgtt cgactacgag tgggcgacct
     2041 tgcagcacca gctcggctgc cgcgccgcga cccgcgtcgt caccccggat gcgatcccga
     2101 tcgctcggct cgcccgcttc ggggccgtgc cgccgaaact ccgccagtac ccgggactga
     2161 aggaggagta ctacctcgcc gacttcgagc ccgactccaa ggctctgcgc tcgctcggcg
     2221 tggacgaggg acgcatcctg gtcgtgctcc gaccgccccc ggacgtgtcc ctctaccacc
     2281 gccactcgaa tccgctcttc ccgcaggtgc tcgaccggtt gggcaacgac ccgaaggtgc
     2341 aggcggtcgt catcccgcgc accgacgagc aacggtggac tatcgccgcc ctgacgctgc
     2401 cgtcgatgat ccttcccgag cgggcggtcg acgcgcagag cctgatcggt ctcgccgacc
     2461 tcgtcgtctc cgcgggcggg acgatgaacc gcgaggccgt cgccctcggg actccggtgt
     2521 acacgaccta cggcggccgc ctgggcgcgg tcgacgagca gctgatccgc gaggacaggc
     2581 tgcgtcccct tgccgccgct cacgacatcg cgctcgagaa gcgctcgagc accgccgagc
     2641 gcgttcgccg cgatccgcgg ctgatgctcg acctgctgct gagctgaccg gccgccggta
     2701 cgggtcgcag ccggcgcgcg gtcacgcgag ccgccacggc taccgttgaa gcgtgggcga
     2761 cagcatcacg ctcggccgct tcttcgggat cccgatcggg atcaactgga gctggctgat
     2821 cgtcttcggg ctgatcacgt ggacgttggc ggactccgtc ttccccgacc agaatcccgg
     2881 tctgtcgagc ggctcctatt ggacgatggc cgttgtcgcc gctgcgctct tcttcgtctc
     2941 gattctgctg cacgagctcg ggcacgccct ccaggcacaa cgggacaacg tgaagatcga
     3001 gggaatcacg ctgtggctct tcggcggcgt cgcacggttc agcggtcgca tacccagtgc
     3061 aggcgccgag tttcggatca ccatcgcagg gcctctcgtc tcgctcgcgc ttgggctcct
     3121 cttctctggc gtcgcactgc tgattgggct cccgtcggct gcagatggcg tcgcagcgtg
     3181 gctcggctac atcaacctag tcctcctggt gttcaatctg ctgcccgcct ttccgctcga
     3241 cggcggccgc gtgctccgct cggcgatctg ggcccggaac ggagaccttg cccgcgcgac
     3301 gaggatcgcc gccgcagtcg gccgggtgat cgccgtcggc ctgatcgtcc tcgccgtcct
     3361 catgctcgtg atcggcaacg tgatcggggg catctggctc ggcttcatcg gctggttcct
     3421 actccaggcg gcaggtgccg agtcgcagca ggcgacggtg accgagcggc tcggcgagct
     3481 caccgtccgc gacctgatga ccgaggcgcc cgtcaccgtg ccgtcacagc tgacgctcga
     3541 ccggttcgtc gacgaactcg tctggacggc ccggttcacg acctaccccg tcgtcgacaa
     3601 cggtgacgtg gtgggcctcc tgcccttccg ctgcgtcgcc gaggtgccgc ggagcgagtg
     3661 gcccaggagg acggtcggga gctgcatgct cgcgctgcgg gacgtacccc agttcgggcc
     3721 cgacaccacg gcctcggacg ccctgccgga tctcgcgtct ggcacggtcg ggcgggcact
     3781 cgtgctcgac ggcggccgcc tcgtgggcct gctctcggca agcgacctcg ctcgggcgct
     3841 cgagctcgac ggctcgggcc gccgctaggc agctagagct cgacgctacc ggcggcccgg
     3901 gtccgaccag ggtcgggaat ccggcgcgac tcgcgcacga tccggttgag cgcgccgact
     3961 gcttcgagcg tgtcgccctc ctccacgagc tgctgcagac gctcgagctc ggcgtcgagc
     4021 cattcggcat ccagcgccgg cctggtgacg agcatgatcg cctcgtgtga gctcggcgag
     4081 acgttctcac cgtcgcctac gagctcctca tgcagcttct cgcccggccg cgcgccgatg
     4141 aactcgatcg agatatcgac tcccggctct ttacccgaga gccggatcat cttctcggcg
     4201 agatccacga tcgacacggg ctcgcccatg tcgagcacgt aaacgtgacc cgactcgccg
     4261 atcgcgccgg cctggatgac gagctgcacc gcctcgggga tcgtcatgaa gaagcgcgtc
     4321 atctcacgat gggtcactgt gagcgggccg ccgttggcga tctggcgtcg aaagatcggg
     4381 atcacgctgc cggacgagcc gagcacgttg ccgaatcgca ccgccacgaa acgcgaggcg
     4441 acttcctcgc gcgcaccgta ggcctcgacg atccattcac acagcgcctt cgtctggccc
     4501 atcaccgtct tggcgttgac cgccttgtct gtcgagacga gtacgaagcg cttgacatcc
     4561 gcgtcgatgg cgacatgggc gatcgtcttg gtgccgatcg tgttgttcct gaccgcctcg
     4621 agcggattcg cctccatcag gggcacgtgc ttgtaggcgg cggcatggaa gacgacgccc
     4681 gggcggtact tgtcgaagac ctggcgcatc ttcgcctcgt tgccgacgtc gcccaggacg
     4741 gcggcagcag catggaaacc ccgctcgtcg acgagctcgc gctcgatctc gaagagtgcg
     4801 ggctcggagt tgtcgaccag gacgagccgg gtaggaccga cacgcgcgat ctgccggcag
     4861 agctccgacc cgatcgaccc accggcgccc gtcaccagca ccacctctcc cgtcaagtag
     4921 ccggcgatcg cgtcgaggtc gacctcgacg ggctctcgcc ccaggacgtc ctcgacctcg
     4981 accggccgaa gctgcccggc gaggttgtag tcgccggaga tcagctcgtt cagacccggc
     5041 agcgtcttga cgggaatgtc ctcgacgcgc gcgatctcga cgatccgctc gcgcagctca
     5101 cccgaggcgg atggaatcgc gatcagcacc tcgtccggcc gccgctcgtg aatgatccgg
     5161 ctcagctcgt cgatcgtgcc gagcaccctg atcccgtgca ggcggagatt gcgcttgcgg
     5221 ggatcgtcgt cgacgagcgc gatcggcgtg taccctagcg ccgggttgcg cagcatctcc
     5281 ttgacgacca gctgcgccgc gtcgccggcg ccgatcacga tcgcctcctt gccccgcgca
     5341 acgatgctgc cgggccgcgg ccgctcgagc agcgttcgcg cggccaggcg cgaaccggcg
     5401 atgagcgcga tgcagagcag cagatcgatg aaccacacgc cgctcggaac acggacacgg
     5461 tgaagctcga ggaacgtgaa gacgaggaac acggccacgg acgcgagcgc gcagccgcgc
     5521 aggatgctcc acatgtcgcg cgtcgagacg tagcgccacc agcggttgta gaagcccgac
     5581 aacgcgaaga ccgggagctt gatcccgacg atgacgagga ggattcccca ctccatgtag
     5641 cggtcgtagt agaccgggcg cccttggtcg aagcgcagct tccaggccag ccaccaggcg
     5701 agaacgacga tggcgccgtc gacggcgacc tgccagaggc tgtgccggct gaacgggaag
     5761 cggagtctca cggcgccgtc acgacgccga cggcggacag caccgcgtcg acgacacgct
     5821 cctgaatctc acggccgata ccgccccaca tcggcagcgc gaggttctct ccagccgccc
     5881 gctcggtctc cggaagggag cccggctccc agcccaggta gcgcatggcg ggctgtaggt
     5941 gcatggggac gacgtagtat gcggcactcg cgattcctgc ctctcggagc cgggacgcga
     6001 tgcgatcgcg ctcggtgctg cggacgacgt acatgtggta aacgtgggcg ggctcgtcga
     6061 ccggcagctc gacggcctcc cccagcccca gctccgcgta gatcgacgct gcctcgcgac
     6121 gggccgcatt ccagcccgaa agacgcggca ggaagacacg aagcgctgca gcgtgaatgg
     6181 cgtcgagcct cgagttggca ccgatcagct cgaacgtctg cttgtcggcc gagccgtgga
     6241 accgcagctt gcgcaccgtc gccgcgacgt cgtcgtcgtt gctggcgacg agcccgccgt
     6301 cgccgagcgc gaacaggttc ttggtgggga agaagctgaa cgtcgagcag acaccggcct
     6361 gggcgacgcc tggtgccccg aaagcctgag cggcgtcttc gaggagcggc acgccgaagg
     6421 cggcgagctc ggcgttcgga gcggggcggc cgaacaggtg cacgggcacg actgccttcg
     6481 ttcgcggcgt gatccggtcg gcgacagcag ctggatcgag gttgagcgtc accggatcga
     6541 tgtcggcgaa gaccggaatc gcacgggtgc gggcgatcgc ctcggcagtg gcgtagaacg
     6601 tgaacgcggg acagatgacc tcgtcgccgg ctccgacacc catcgcttcg agcgccagca
     6661 ccaacgcgtc ggttccgttg gcgacgccga tcgcatgcgg cacgccgagg tacgccgccg
     6721 cttcgcgctc gaacgcctcg acctccgggc cgaagatgaa gcggcccgag tcgagcaccg
     6781 cggcgaagcg ctccttgaga tccggtatga gcggcgcgta ctgcgccttg acgtcgacga
     6841 gcgggatcat cagtccgacc cgggagtgta gcccggggcg ggctcgtttc ccgcctttcg
     6901 ccgccgtagc agccgccagc ccaccagcgc gatcacgacg acgactgcgg cgatgacgac
     6961 gtagtcgacg taccggaagg cgtcgtggaa gcgctcccag ttcgagccga gcgcgaagcc
     7021 gatcccaccc aagagcaggc accagatcgt cgagccggcg aaggtcagca gcgtgtagcg
     7081 gccgagcggg gcgcggaaga caccggcggg cacggagatg aacgatctga cgaccggtgt
     7141 gaggcgaccg aggaagaccg cccagtcctc ccagcgctcg aaccaccgat ccgcccgctc
     7201 gagcttggcg ttgtcgaggt ggagccagcg cccgtgtctc tccaggtacg gccgccccgc
     7261 gtagtcgccg attgcccagc ccaggatcga gccgatcgtg tagccgatcg tcccggcgag
     7321 gacgacggcg acgaatgcgc cgaacccctc ggaaagctcc cagccgaaca acgtgaccgt
     7381 ctgtgacgtg aacgcaccgg cggcgagcgc gccggcatag accatgatca gctcgctcgc
     7441 tgccggcagg actgcgtcca ccagcatgag caggaagatc gcatacagcc cgtagtcacc
     7501 gatcgtcccg gtgatccagt ggctgatgtc ggtcaggatc gaagcaacgg gcacgacggc
     7561 actctagtca gcgacccggc aggtgcttct cgaccgccct cgccgtccgg cgaccaggcg
     7621 gctaggcgtc gtcgacaccg gtatcgaggg cacgcttggg ccccttgatt cgctgcacac
     7681 ccagcttgcg ctcgagctcc acgttgccaa gcgcgttgcg ggccagcacg tgtacgcggt
     7741 acaggccgcc cttcaccggc ttgcccttgc gatccaggcc gtcccagatg acccgacgga
     7801 gacccttgga gtaccggcgc attgtgaagg agcgaacgat ctcgccgttc gtgctctcga
     7861 tccacacgcg gacgcgggcc ggtctgccga gacgccagcg gatctcgagc tcccggccct
     7921 ccggcgggag gaagaggcgc cgcgggctcg tgcgcacgaa gccgagcgtc gtgttgagct
     7981 tgaaggagcg cgccatcttc gacacctgcc cgtcttcgtc cacggccctt acggtgaccc
     8041 gccatttgcc ctccttcagc gacccgacgt ccgggtagag ctgcggcgcc ctgctcgtcg
     8101 aggtcgacag cggagcatgc gttgcgcccg c
//
LOCUS       DBNGNK010000125         4617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1525523,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000125 DBNGNK010000000
VERSION     DBNGNK010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1525523"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC150V_03620"
     CDS             complement(<1..244)
                     /locus_tag="AC150V_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762040.1"
                     /translation="MSTLRRLALLAGASRRRVALSVFLGASAIAFGIGLMATAGYLIS
                     RAAEQPPILSLTVAIVAVRFFGTSRPLVRYVERLASH"
     gene            complement(241..1917)
                     /gene="cydD"
                     /locus_tag="AC150V_03625"
     CDS             complement(241..1917)
                     /gene="cydD"
                     /locus_tag="AC150V_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0050456 - L-cystine reductase (NADH)
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HIR3762041.1"
                     /translation="MRLLDRRLLERAQPARRLLVADTALGVAAALLILAQAVLLARIV
                     ARVFGGAGLDDVTLEIALLAGAIALRAALVWAFEAAGRLAAAAVLSELRLSLVRSRLR
                     DRPSSTDGAQTGEIAAAGVQGIDALEAYFARYLPQVVLAAVVPVAVLALAVVVDPLSA
                     GLMLVTLPLVPIFMVLIGKLTERRTRRRWRALATLSNHFLDVVRGLPTLRAFNRGEAQ
                     AERIATVNDDYRRATMGTLRVAFLSGAVLELAATLGVALVAVTVGVRLVEGNIGFQAA
                     LTVLVLVPELYLPLRNLAAQYHASADGVAVADRLLSLTAPAARGRSPGARPPRADVAA
                     IRFENVSFAYPSRPVDVLHSVDLEIPPGETTALVGPSGGGKSTVVALLLGFVEPAAGR
                     LTVGGEDLAALDLDEWRRQIAWVPQSPTLFRGSIAANIALGDHAADDRAIRRAADRAG
                     AAGFIDALPAGLDTRVGDGGRPLSAGQRQRIALARAFLRDAPLLLLDEPTANLDDGSA
                     EIVAAALAQLAGRTVLLIAHRPELAARAAASRIVRVGDGRVAELPLGAAR"
     gene            complement(1924..3000)
                     /gene="cydB"
                     /locus_tag="AC150V_03630"
     CDS             complement(1924..3000)
                     /gene="cydB"
                     /locus_tag="AC150V_03630"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR3762042.1"
                     /translation="MDLETLWFCLICVLWTGYFVLEGFDFGVGMLLPVLGRTESDKDA
                     MLGSIEPVWDGNEVWLLVAGGATFAAFPQWYATMLSGFYLALLLILVLLIIRVVSFEW
                     RGKVSGSGARSAWTWVNTISSVGAPALWAIALSSLLYGVPIDADKEYAGSFWDLFSAY
                     TVVAGLSVVLLFALHGATFLTLRTTSQLRERAARLSARLSPVALVAGGAFLIWTFVVG
                     IDRNDKGVFPGIVVVVAAAAAAAGAVVLSRTGRAGRAFAATAATIGLAVVTLFTMLYP
                     RVMVSVPEFGNSLTIENAASGSYTLTVMTIAAGVLLPIVLLYQGWSYHVFRARLGHTE
                     AVKNPADLLVRGEESSTRDPDSRG"
     gene            complement(3000..4418)
                     /locus_tag="AC150V_03635"
     CDS             complement(3000..4418)
                     /locus_tag="AC150V_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR3762043.1"
                     /translation="MDSVPLASALELARWQFALTTLYHFIFVPLTLGLAPLLAVMQTL
                     AYRRREERERWERLVRFFGVLFLINFAIGAATGLVQEFQFGMNWSTFSRYVGDVFGSP
                     LAIEGLGAFMLESTFIGLWVFGRGRLKPRVHLATIYLVWLGTWLSAYFIIAANSWMQH
                     PIGYELNKQLDRVEANDIGKILFQPFAIVAWGHAILAALMTAGFLVLGVSCWHLWRRR
                     NVDLFHSAAKLAIVVVLPVTALNLAWGSEFGVFITDVQPMKIAATEALWETEQPAGFS
                     LFQIGGFSADDQTPSFDIQVPRLLSFLATNSLDGKVVGMNELQAQSEQQFGPGNYIPD
                     VRLVYWSMRVMAYLGTLSFLLAAWAGWLLWRGRLEQTTWFHRAAIAGITLPFLANFAG
                     WILTEAGRQPWVVYGLLKTEDAVSPTVSAWTVGLSLAVFMTLYLVLAVVDFVLMRRYA
                     RLDPPELQGDDAEGAPLSAPSY"
BASE COUNT          848 a         1551 c         1627 g          591 t
ORIGIN      
        1 cgtgcgaggc aaggcgctct acgtaccgga cgagcgggcg agaggtcccg aagaagcgga
       61 cggcgacgat cgccacggtc agggagagga tcggcggctg ctcggcggcg cgagagatca
      121 ggtagccggc tgtggccatc aaaccgatgc cgaacgcgat cgccgaggcc ccgaggaaga
      181 cggacagcgc gactcgccgg cgcgaggcgc ctgcgagcag cgcaagacgc ctgagggtgc
      241 tcaccgcgcc gctccgagag ggagctcggc gacgcggccg tcgccgactc ggacgatccg
      301 gcttgctgcg gcccgggctg cgagctcggg ccggtgcgcg atcagcagga cggttcggcc
      361 ggccagctgc gcgagcgccg ccgcgacgat ctcggcgctc ccatcgtcga ggttcgccgt
      421 cggctcgtcg agaaggagca gaggagcgtc gcgcaggaac gcccgggcga gtgcgatccg
      481 ctgtcgctgg ccggccgaca gcggccggcc gccgtcgccc acgcgcgtgt cgagcccggc
      541 gggcagcgcg tcgatgaagc ccgcagcacc cgcccgatcc gcagcccgcc ggatagcccg
      601 atcgtcggcg gcgtgatcgc cgagcgcgat gttggcggcg atcgagccgc gaaagagagt
      661 cgggctctgc ggaacccacg cgatctgccg tcgccactcg tcgaggtcga gtgcggcgag
      721 gtcctctccg cccacggtca gccggccggc ggccggctcg acgaaaccga ggagaagtgc
      781 cacgacggtg ctcttgccgc ctccgctcgg cccgacgaga gcggtcgtct ctcccggcgg
      841 gatctccagg tcgacggagt gaagtacgtc gacgggtcgt gacggatagg cgaacgacac
      901 gttctcgaag cggatcgccg cgacgtcggc ccggggcggc ctggcgcccg gcgaccggcc
      961 ccgcgccgcc ggagcggtca ggctcaagag ccggtcggcg accgcgactc cgtcggcgct
     1021 tgcgtggtat tgcgccgcga ggttgcggag gggcagatac agctcaggca cgagcacgag
     1081 taccgtcagc gccgcctgga agccgatgtt tccttcgacc aggcgcacgc cgaccgtcac
     1141 cgcgaccagc gctactccca gcgttgccgc cagttcgagc actgctcctg agagaaacgc
     1201 gacgcgcaac gtgcccatgg ttgctcgccg atagtcgtcg ttgacggtcg cgattcgctc
     1261 ggcctgagct tcgccgcggt tgaaggcccg cagcgtcggc agtccgcgca cgacgtcgag
     1321 gaagtgattc gagagcgtcg ccagcgcgcg ccaccggcgc cgcgtccgcc gctcggtgag
     1381 cttgccgatc aacaccatga agatcggtac gagcggcagc gtgacgagca tcagccctgc
     1441 agacagcgga tcgacgacga ccgcgagcgc gaggaccgcg accggtacga ccgcggccag
     1501 cacgacctgt ggcaaatagc gggcgaaata cgcctcgagg gcgtcgattc cctgtacacc
     1561 ggcagccgcg atctctccgg tctgggcacc gtccgtgctc gacggacggt cgcgaaggcg
     1621 actccgtacg agcgagagcc tgagctcgga gagcaccgcg gcggccgcta gccggccggc
     1681 ggcctcgaag gcccacacga gcgcggcgcg cagcgcgatc gctcccgcga gcaacgcgat
     1741 ctcgagcgtg acgtcgtcga ggcccgcacc gccgaagacg cgagcgacga tccgggcgag
     1801 gagcaccgcc tgggcgagga tcagaagcgc cgcggcgaca ccgagcgcag tgtccgcgac
     1861 gagcagccgg cgtgccggtt gggctcgttc gagcaggcgg cggtcgagca ggcgcacgag
     1921 cggctagccg cgcgagtccg gatcgcgtgt ggagctctct tctccccgga cgagcaggtc
     1981 ggcggggttc ttcacggcct ccgtgtgacc gagtcgtgcg cggaaaacat ggtaggacca
     2041 gccctggtag aggaggacga tcgggagcag aacgccggcg gcgatcgtca tcacggtgag
     2101 cgtgtagcta ccggacgccg cgttctcgat cgtgaggctg ttgccgaact caggcaccga
     2161 gaccatcacg cgggggtaga gcatggtgaa gagcgtgaca acggccaggc cgatcgtggc
     2221 ggcggtcgcg gcgaaggcgc gcccggctcg gcccgtccgg ctcagcacga ccgcgccggc
     2281 cgccgctgct gccgccgcga ccacgacgac gatccccggg aagacgccct tgtcgttgcg
     2341 gtcgatcccg acgacgaagg tccagatcag aaacgcgccg ccggcgacga gggcgaccgg
     2401 cgacagccgt gccgacagcc gagccgctcg ctcacgaagc tggctcgtag tccgcagcgt
     2461 caggaaggtc gcgccgtgaa gcgcgaagag gagcacgaca ctgagccccg cgaccaccgt
     2521 gtaggcgctg aacagatccc agaacgagcc ggcgtactcc ttgtcggcgt cgatcgggac
     2581 gccgtagagg aggctcgaca aagcgatcgc ccacagtgcc ggagctccga ccgaggagat
     2641 cgtgttgacc caggtccagg cactccgggc gccgcttccg ctcaccttgc cgcgccactc
     2701 gaaggagacg acgcggatga tcagcaggac gaggatcagc agcagggcga ggtagaagcc
     2761 ggagagcatc gtcgcgtacc actgagggaa ggccgcgaag gtggcaccgc cggcgacgag
     2821 cagccacact tcgttgccgt cccagaccgg ctcgatcgat ccgagcatgg cgtccttgtc
     2881 gctctccgtc cgaccgagca ccgggagcaa catcccgact ccgaagtcaa agccttccag
     2941 cacgaagtat cccgtccaga ggacacagat aaggcagaac cagagagtct cgagatccat
     3001 cagtagctcg gagcagagag cggggcaccc tccgcgtcgt caccctggag ctccggcggg
     3061 tcgagccgcg cgtagcggcg catgagcacg aagtcgacga cggcgagcac aaggtaaagg
     3121 gtcatgaaca cggcgaggct gagaccgacg gtccacgcgc tcacggtcgg cgagaccgcg
     3181 tcctccgttt tcaagaggcc gtacacgacc cagggctggc gaccggcctc ggtcaggatc
     3241 cagccggcga agttggcgag aaagggcagc gtgatgccgg cgatcgcggc tcggtggaac
     3301 cacgtcgtct gctcgagccg gccgcgccac agcagccaac cggcccacgc tgcgagcagg
     3361 aacgagagcg ttccgagata ggccatcaca cgcatgctcc agtacacgag ccggacgtcg
     3421 gggatgtagt tgcccgggcc gaactgctgc tcgctctgcg cctgcagctc gttcatcccg
     3481 accacctttc cgtccaggga gttcgtggcg aggaacgaga gcaggcgggg aacctggatg
     3541 tcgaacgacg gcgtctggtc gtcggcgctg aagccgccga tctggaacag cgagaagccg
     3601 gccggctgct ccgtctccca gagcgcctcg gtggcggcga tcttcatcgg ctgcacatcg
     3661 gtgatgaaga cgccgaactc gctgccccac gcgaggttga gcgcggtcac gggaaggacg
     3721 acgacgatcg cgagcttggc tgcgctgtgg aacaggtcga cgttccgacg gcgccacagg
     3781 tgccagcagg aaacgccgag aaccaggaac ccggccgtca tcagcgcggc gagaatggcg
     3841 tgcccccagg cgacgatggc gaagggctgg aagaggatct tcccgatgtc attcgcctcc
     3901 acccggtcga gctgcttgtt gagctcgtag ccgatcgggt gctgcatcca cgagttggcg
     3961 gcgatgatga agtacgcgga aagccaggtg ccgagccaca cgaggtagat cgtcgcgagg
     4021 tgcacccgcg gcttgagccg gccgcgaccg aacacccaga ggccgatgaa cgtcgactcg
     4081 agcatgaacg cgcccagccc ttcgatcgcg agcggcgagc cgaagacgtc accgacgtag
     4141 cgcgagaacg tcgaccagtt catgccgaac tggaactcct ggacgagccc cgtcgcggca
     4201 ccgatcgcga agttgatcag gaacagcacg ccgaagaagc gcaccaggcg ctcccagcgc
     4261 tccctctcct cgcgacggcg gtaggcgagc gtctgcatca ccgccagcag cggcgcaagc
     4321 ccgagcgtca gcgggacgaa gatgaagtgg tacagggtcg tgagcgcgaa ctgccacctc
     4381 gcgagctcga gtgcgctcgc gagcggcacg gaatccatca cggtgatgtc aaccggtgca
     4441 cggccacaaa gagttctgtg actctaacga ggctggccaa cacccggtcg acgctgtacg
     4501 ttccaccagc cggagcggtc atggctcatc ccgacctcgc cccgatcccg cagagccagc
     4561 tcgcacagcc gctgctcctt cccgacctgc tcgactggct gggtgcgagc gcgacgt
//
LOCUS       DBNGNK010000126        11574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_593441,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000126 DBNGNK010000000
VERSION     DBNGNK010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_593441"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="AC150V_03640"
     CDS             complement(<1..683)
                     /locus_tag="AC150V_03640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005321424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762044.1"
                     /translation="MCVTEINVEQSDAFAGRMLEIVNDGLLALQISVASQVGLFETLA
                     GAGPLTVAEAAADESLDERYVRELLGGLVVGKILTYDPAEETYELPTEHAAFLTAAAG
                     PDNLATFTQYVALLGEVEQDVVRCVREGGGVPYSKQPRFQAIQAAETSRVYDATLIDA
                     QLPLAPGLVERLRSGIDVLEIGCGQGHASNLMADAFPASRFKAFDISEEGIAVGRAEA
                     ARLGLTNLEL"
     gene            complement(772..2391)
                     /locus_tag="AC150V_03645"
     CDS             complement(772..2391)
                     /locus_tag="AC150V_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3762045.1"
                     /translation="MEREAELIEEGRVALARGDWPEARTRFEEALANGEAVEALEGLG
                     IAARMQQDGEAAVTAHERAYRAARATGDVQSAARIAIELAYDSYSFRGVAETNGWLER
                     ASQLLETLPASTESAMVAHMRGHLAMLVRNDPEGARAFAAEALTLARATESVNVEMLA
                     LGLEGLALVSVGSVEDGMRRLDAATVAAVSGEVTDLECVEAICCYLIDACKRVRDFDR
                     AAEWCLRVEELSERYERREMFAICRTHYADVLLWRGEWEQADAALTRASEELARSRES
                     KAADSEVRLAELRRRQGRLDEADAILERLDGAHRLTSLHRGLLALDRARPELAVEEAE
                     RFLRRIGEGDRFERVAGLELLARGSLAAGDPARARRAADELAAIAGSVGTYPLAAARA
                     AAVGRLALAEGDVAAASDALEDAVDLYAACGAPYEEATTKLALAVAWRELGRADEADD
                     AEHQALAQLKGLGAAAPAPTNEAGPLSAREREVLVLVAAGRSNEEIAKELVLSVRTVE
                     RHVANIYAKIGVSGRSARAAATSWAHAHGIT"
     gene            complement(2425..3288)
                     /locus_tag="AC150V_03650"
     CDS             complement(2425..3288)
                     /locus_tag="AC150V_03650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3762046.1"
                     /translation="MAASPLSPIDLRHAGNDRIIGAYLIETADGPALFDCGPATCVAR
                     LLEGLAERNVALTDVRHLLLSHIHLDHAGAAGVLVREHPRLQVHVSEIGAPHIVDPSR
                     LESSARRLYGDAFDVLWGELVPVPRENVHVVGNDVLGLSAFPTPGHAAHHVSYLAADG
                     TLYSGDAAGVRVTPGRFVLPPLPPPEIDLEAWEATIAETERRAPARLALIHFGVFEDV
                     TEHLSRLRETLRRWGSWVEDGMDEETFVAAARAEVEAVDWEASESYEHATPYWHCYRG
                     LERYWRKKRER"
     gene            complement(3488..4651)
                     /locus_tag="AC150V_03655"
     CDS             complement(3488..4651)
                     /locus_tag="AC150V_03655"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HIR3762047.1"
                     /translation="MATIRPVEFETRAIHVGQDPDPATGSVTTPIYQTSTFAHEAVGV
                     HKGYEYARVSNPTRTALEECLASLEGAEWGHAFASGLGAATTIMHLFQPGDRIVAVND
                     VYGGTYRMFSQVYEPKGYAFTYLSPGEINNELSAHLDDSVRLVWLETPTNPLLNLVDI
                     RAAADAAHAAGALVLVDNTFATPYLQRPLELGADIVLHSTTKYLGGHSDVIGGFVATN
                     DASISERVRFLQKSLGAVPGPFDAWLVLRGLKTLAVRMERHCSNAMAVAELLQAHDRV
                     ARVFYPGLPEHPGHEIAAAQMSGSGGMVSFTLGDEEEAVDLVARTNLFVLAESLGGVE
                     SLIEHPYRMTHASTADAPIAVPRDLIRLSVGIEAQDDLLTDLEQALAAVPART"
     gene            complement(4698..5828)
                     /locus_tag="AC150V_03660"
     CDS             complement(4698..5828)
                     /locus_tag="AC150V_03660"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR3762048.1"
                     /translation="MRTVTVTVLRRLAVNAQGMASRSRRGTLRETEEAIRRLSCVQLD
                     SISTVERSHRIVLGARVGDYPRGGVDRLLRQGRVFEYWAHEACLLPVEDWPLCRAAMQ
                     AGGRNWYGDVERTHPHLHDEILAEIEARGPLPSRHFDGAGRGMWNWKPAKAMLERLWN
                     RGELVIAGRQGFQRLYDLPERVIPRDVLAAPAPDEAELLRILTLKAVRARGALTDAGI
                     VEHWRLSGGTKRIRPTVDALVADGRLERVSVDDGGPPVVVPAGIELEPERPTAAVLLS
                     PFDNLLWDRRFARRALGFDHVIESYKPAPERRYGYYVLPLLWRDRIVGRADLKSERGD
                     GALVLKAFHRENRVRASAALDDALDRALARLRRVIGLERVQR"
     gene            5872..6399
                     /locus_tag="AC150V_03665"
     CDS             5872..6399
                     /locus_tag="AC150V_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3762049.1"
                     /translation="MRRRILAAVLLFVAMAVPSASLFVLRDDDELPERADAVVVLAGS
                     ASRLPVALDLIERGVAPVLVVSDPEGRRDDDRRDLCAAGSDEYELICVLPDPYSTRGE
                     ARMVARLASERGWESIVVVTSRFHLFRAKRLFERCFGGEVALVGSPVSWWYRPIAVAS
                     EWAKLAVAETFRRGC"
     gene            complement(6405..7403)
                     /locus_tag="AC150V_03670"
     CDS             complement(6405..7403)
                     /locus_tag="AC150V_03670"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3762050.1"
                     /translation="MSRPRGRKEPGRRGAVLIPLADITAARETIRDVVVRTPLVRLDA
                     PESPAEIWLKLENLQPIGSFKLRGATNTIRRAPRGALERGVVTASAGNMAQGVAWVAR
                     ELGLPATIVVPGHAPEAKLAAIDRLGGHIVKVPYERWWQALEESRFDDAPGFFVHPVM
                     DERVMAGNGTIGLELAEQLGEIDTVLIPWGGGGLTTGIASALSALSPRTKVFACEPET
                     GAPVSAALEAGRPVTVDYRPSFVDGAGSKALLEPMWERARDLIAGAHVVSLEAAAAAV
                     RLLAERGRVVAEGAAALPVAAALANAEPSQRVVCIVSGGNIDSSRLAAILQGGVPD"
     gene            complement(7352..8320)
                     /locus_tag="AC150V_03675"
     CDS             complement(7352..8320)
                     /locus_tag="AC150V_03675"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3762051.1"
                     /translation="MSVLVLSGDDVRAVLDMPSCISAMEGALAALAREELSMPLRFVM
                     RPPGESRLMGFMPAHRGGDRPLFTLKEIVIAPGNSARGLDPHQGAVLVHDGATGQLRA
                     VLDASAVTEIRTAAVSAVATRLLARADAASVAILGAGVQGRSHIEAMRAVLPGVEVRV
                     WSRSRGRAERLAAKENVVLAGSAEEAVAGADVVCTCTSSREPVLRRAWLAEGAHVNAV
                     GASIPTARELDAETISAAALFVDRRESTLNEAGDYLMAVEEAGIGPEHIRAELGELLT
                     GAVEGRGSVEELTVFKSLGLAVEDLAAAELCLARAAERSLGVEVPF"
     gene            complement(8317..9291)
                     /locus_tag="AC150V_03680"
     CDS             complement(8317..9291)
                     /locus_tag="AC150V_03680"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3762052.1"
                     /translation="MEARELGQSGISVSRIILGCGGFGGIGSAPQFFGQGTSRDEAHR
                     ILDAAWDLGITTLDTADAYGGGRSESFIGEWLATRSRDVRDRAIVTTKTFNPMAEGAD
                     RGLSRARIVRQVETSLERLALERIPLYMTHDWDEETPIEETLGALDGLVASGKVGAIG
                     ASNISAEHLAEAVEISRLEGIARFEWVQNAFSLLELGDAQTVFPVCHEHGLGFTPFSP
                     LAGGWLTGKYRRGEPFPAGSRMTLRPEGSEGYAADAIFDALEAFERAAAERGISIAGL
                     ALAWALAVPEITAVIVGPGKVEHLSPVKEAQSASLSAAERDRLTALFP"
     gene            complement(9301..10509)
                     /locus_tag="AC150V_03685"
     CDS             complement(9301..10509)
                     /locus_tag="AC150V_03685"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762053.1"
                     /translation="MRRTHVWLDRAVPSERLLTRPFAVNWAATFLLLLSIGTLLPVLP
                     LYAKGPLGASGLGIGLAVAAASPTAFLFQPLGGRFGDRRGRRILIVGGGVIAALSIAA
                     YTGAESLPLLVLLRLVTGVGEALIFVGTATVVNDLAPDKRRGEALSLYSLAVWGGLAV
                     GPLLGELVLADDRFDAVWLTAAAFALAGGLVGIALPETRPATDGREKAGRLFSRAATF
                     PGLALAASMVGFAGFAAFVPIYARDIGMSGAGTVFAVNAAVVVAIRSFGRQIPDRVGP
                     KRTATLALALVASGFLAIALSSSPAGLYAGTVVLAAGQALVFPALMTLAVAAASIAER
                     SAVVGSFTAFADLGFAAGAIGLGAVETTAGVDGLFVVAACVAALGLLPLSRVRRRPAV
                     PVPAAVDAPP"
     gene            complement(10604..10984)
                     /locus_tag="AC150V_03690"
     CDS             complement(10604..10984)
                     /locus_tag="AC150V_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3762054.1"
                     /translation="MRAAGILIAVVLLLLLVLAACGGGNGDGESASPPPPPAETETGQ
                     PENGGTSADGAALFASSGCGGCHTLSAAGSTASIGPNLDETSLTAEQIEVQVREESGA
                     MPAFGELLSDEEIQAVAAYVAGSS"
     gene            complement(11020..>11574)
                     /locus_tag="AC150V_03695"
     CDS             complement(11020..>11574)
                     /locus_tag="AC150V_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762055.1"
                     /translation="LVGLTIVQAATARAMVTIDAGEPVSARVVYLSVLKKVWTLIWAL
                     VRVVVVVGILNLTAIGIPFAIWLVGRWAFIGQVIELEGDSREHVLRRSSRLVSGRWWK
                     VASIAIVMTTVALVSGPFVGALMLLVTDASFNFVNVIAAVIYTFLIPLAAIGTTYVYF
                     DCRVRDELDVDELTERSKVLAAEI"
BASE COUNT         1731 a         4059 c         4086 g         1698 t
ORIGIN      
        1 agctccagat tggtcagccc cagccgcgcc gcctcggcgc ggccgaccgc gatgccctcc
       61 tccgagatgt cgaaggcctt gaaacggctc gcggggaacg cgtcagccat caggttcgag
      121 gcgtgtccct gaccgcagcc gatctcgagc acgtcgatac ccgagcgcag ccgctcgacc
      181 agaccgggcg cgaggggcag ctgggcgtcg atcagggtgg cgtcgtagac gcgagaggtc
      241 tcggcggcct ggattgcctg gaagcgcggc tgcttgctgt aggggacgcc gcccccttcg
      301 cggacgcagc ggacgacgtc ttgctcgact tccccgagca gagccacgta ctgggtgaac
      361 gtcgcaaggt tgtccggccc cgccgccgcg gtcagaaacg cggcatgctc ggtcgggagc
      421 tcgtaggtct cctcggcggg atcgtaggta aggatcttgc cgacgacgag gccgccgagc
      481 agctctcgga cataccgctc gtcgaggctc tcgtccgcgg ctgcctccgc cactgtgagc
      541 ggacctgcgc cggccagggt ctcgaagagg ccgacttgcg aggcgacact gatttgcagc
      601 gcgagcaggc cgtcgttgac gatctcgagc atccggcccg cgaaggcatc ggactgctca
      661 acgttgatct cggtgacgca catggggatc tccttcaggt gggttcgtca ccagcatgcg
      721 ccgccgcttc cgcgcgcaca tcggtgccgg tacccaatct gacggcgggc gctacgtaat
      781 cccgtgtgcg tgtgcccacg acgtcgcagc cgcccgcgcg ctgcggccgg agacgccgat
      841 cttggcgtag atgttcgcca cgtggcgctc gacggttcgg acgctgagta cgagctcctt
      901 ggcgatctcc tcgttcgatc tcccggcggc gacgagtacg agcacctcac gctcgcgcgc
      961 actcagcggg ccggcttcgt ttgtcggggc cggggcggcc gcgccgaggc ccttgagctg
     1021 tgccagcgcc tggtgctcgg cgtcgtcagc ctcgtccgct cgtccgagct cgcgccacgc
     1081 aacggccagc gccagcttcg tggtggcctc ttcgtacggg gcgccacagg cggcgtacag
     1141 atccacggcg tcctcgagcg cgtcgctggc cgcggcgacg tctccctcgg cgagtgcgag
     1201 gcggcccacg gcggctgcac gggctgcggc cagtggatag gtcccaaccg accccgcgat
     1261 cgcggccagc tcgtcggcgg cgcggcgggc ccgggcggga tcgccggccg cgagcgagcc
     1321 gcgcgcgagg agctcgagtc ccgcgacgcg ttcgaatcgg tcgccctcgc cgattcggcg
     1381 caagaagcgt tcggcctcct cgacagccag ctcgggccga gcgcggtcga gtgcgagcag
     1441 gcccctgtgc agcgaggtga gtcgatgtgc gccgtcgagc cgctcgagga tcgcgtcggc
     1501 ctcgtcgagc ctcccctggc gtcgtcgaag ctcggcgagt cgaacctcgc tatccgccgc
     1561 cttcgactcg cgtgagcggg cgagctcctc ggaagccctg gtgagtgccg cgtctgcctg
     1621 ctcccactcg ccgcgccata gcaggacgtc cgcgtaatgg gttcggcaga tcgcgaacat
     1681 ctcgcggcgc tcgtaccgct ccgacaactc ctcgactcga agacaccatt cggcggctcg
     1741 atcgaagtcc cgcacccgct tgcaggcgtc gatcaggtag cagcagatcg cctccacgca
     1801 ttccagatcg gtgacctcgc cggagaccgc cgccacggtc gccgcgtcga gccgacgcat
     1861 gccgtcctcg acggatccca cggacacgag agccagccct tcgaggccca acgcgagcat
     1921 ctcgacgttc acggactcgg tcgcgcgggc cagggtcaag gcctcggcag cgaacgcgcg
     1981 cgccccctcg gggtcgttgc ggaccagcat ggcgaggtgg cctcgcatgt gcgcgaccat
     2041 tgcgctctcg gtcgaggcgg gaagcgtctc gaggagctgc gacgctcgct ccagccagcc
     2101 gttcgtctcg gccacaccgc ggaacgagta ggagtcgtaa gcgagctcga tcgcaatccg
     2161 ggcggcggac tggacgtcgc cggtagcacg cgcggcccgg taggcgcgtt cgtgggcagt
     2221 gacggcagct tcgccgtcct gctgcattcg cgccgcgata ccgaggcctt cgagcgcttc
     2281 gacggcctct ccgtttgcga gcgcctcctc gaagcgggta cgcgcctccg gccagtcgcc
     2341 ccgggcgagt gcgacacggc cctcctcgat cagctccgct tcgcgctcca ccccgttgag
     2401 gatagagccg cggcatgcgc ggagctatcg ctcgcgcttc ttccgccagt agcgctcgag
     2461 gcctcggtag cagtgccagt acggcgtagc gtgctcgtac gactcggacg cctcccagtc
     2521 gactgcctcg acctcggcgc gggcggcggc gacgaacgtc tcttcgtcca tcccgtcttc
     2581 gacccagctc ccccaacggc gcagtgtctc gcgcagtcgc gacaggtgct cggtcacgtc
     2641 ctcgaagacg ccgaagtgaa tgagggcgag gcgcgcgggg gcccgacgct cggtctcggc
     2701 gatcgtcgcc tcccacgctt cgaggtcgat ctcgggcggc ggcaggggtg ggaggacgaa
     2761 tcggcccggt gtcacgcgga cgccggcggc gtcgccggag tacaacgttc cgtccgctgc
     2821 caggtacgag acgtggtggg ccgcgtggcc gggcgtgggg aatgcgctga gtcccagcac
     2881 gtcgttgccg accacatgaa cgttctcccg cgggacggga accagctcgc cccagaggac
     2941 gtcgaaagca tcgccgtaca ggcggcgcgc gctcgactcg agccggctcg ggtcgacaat
     3001 gtgcggcgcg ccgatctccg atacgtgaac ctgcagcctc gggtgctcgc gcacgaggac
     3061 gccggcggcg ccagcgtggt cgagatggat gtgcgagagg agcaggtggc gcacgtcggt
     3121 gagcgcgacg ttccgttcgg cgagaccctc gaggagccgg gcgacgcagg tggccgggcc
     3181 acagtcgaaa agcgccggcc catcggcggt ctcgatcagg tacgcgccga taatccggtc
     3241 gttacccgca tggcggagat cgatcggcga taggggggaa gcggccacgg cgcggacgct
     3301 atccgacggt cggcgagtcg gctcgggcgt gggcagttcg tgatggtccc ggcacgcttc
     3361 ggccgcacat gcatgcggac ttcacgagta cgctcgtgac gtggggagga ggacggtggc
     3421 ccccagggcc cccgctcagc cgccgtgtcc gcttcggccg cggttcgcgg gcgcttggct
     3481 ccggcggtca ggtacgagcg ggaactgccg cgagtgcctg ctcgaggtcg gtaagcagat
     3541 cgtcctgtgc ctcgatcccg accgagagcc ggatgaggtc gcggggaact gcgattggcg
     3601 cgtcggcggt ggaggcgtgc gtcattcggt agggatgctc gatcaggctc tcgactccgc
     3661 cgaggctctc cgcgaggacg aacagattcg tgcgggcgac gagatcgaca gcctcctctt
     3721 cgtcccccag cgtgaaggac accattcctc cagagccgct catctgcgct gccgcgatct
     3781 cgtgcccggg gtgctcggga aggcccggat agaagacgcg cgcgacccgg tcgtgtgcct
     3841 ggagaagctc ggccaccgcc atcgcgttcg agcagtgccg ttccatcctc accgcgagcg
     3901 tcttcaggcc gcgtagcacc agccaggcgt cgaagggccc cggcacggcg ccgagcgatt
     3961 tctgcagaaa gcgaacccgc tccgagatcg acgcatcatt cgtcgcgacg aagccgccga
     4021 tcacgtccga gtggccaccg aggtacttcg tcgtcgagtg gagcacgatg tcggccccga
     4081 gctcgagcgg ccgctgcagg tacggcgtcg cgaaggtgtt gtcgacgagg acgagcgcgc
     4141 cggccgcgtg cgccgcgtcg gcggcggcgc ggatgtcgac gagattcaga aggggattcg
     4201 ttggagtctc gagccagacg agacgcacgc tgtcgtcaag gtgtgcagag agctcgttgt
     4261 tgatctcccc gggcgacaga tacgtgaatg cgtacccctt cggctcgtag acctgcgaga
     4321 acatgcggta cgtcccgccg tagacgtcgt tgaccgcgac gatccggtca ccgggctgaa
     4381 agaggtgcat gatcgtcgtc gccgcaccca gaccggacgc gaacgcgtgc ccccactcgg
     4441 ctccctccag cgacgccagg cactcctcga gcgctgtgcg ggtcggattc gagacgcgcg
     4501 cgtactcgta ccccttgtgc accccgacgg cttcgtgggc gaaggtcgac gtctggtaga
     4561 tcggggtcgt aaccgacccg gtcgccgggt caggatcctg cccgacatgg atggcccggg
     4621 tctcgaactc caccggccgg atcgtagcca tcccccgcgg ccgctcgcag cgcgggctcc
     4681 ttctcacagg ctcggtgcta gcgctgtacg cgctcgagcc cgatcacgcg gcggagccga
     4741 gcgagtgctc ggtcgagtgc atcgtcgagt gccgccgacg cgcggacgcg gttttcgcgg
     4801 tggaaggcct tcagcaccag ggctccgtcg ccgcgctcgg acttgagatc ggcgcggccg
     4861 acgatgcggt cacgccagag caatggcagg acgtagtagc cgtaccgccg ctcgggcgca
     4921 ggcttgtagg actcgatcac gtggtcgaag ccgagcgccc gacgcgcgaa ccgccgatcc
     4981 cagagcaggt tgtcgaacgg tgagaggaga actgcagccg tcggtcgttc gggctcgagc
     5041 tcgattccgg ccggaacgac caccggcggt ccgccgtcgt cgaccgagac gcgctcgagc
     5101 cgaccgtctg cgacgagcgc gtcgacggtc ggtcggatcc gtttcgtccc gccgctcagc
     5161 cgccagtgct cgacgatgcc ggcgtcggtc agcgcgccgc gtgcccgcac cgccttgagc
     5221 gtcaggatcc gcaggagctc ggcttcgtcg ggggccggcg cggcgagcac gtcgcgtgga
     5281 atcacccgct cgggcaggtc gtacaggcgc tgaaagccct ggcgtccggc aatcacgagc
     5341 tctcccctgt tccagaggcg ctcgagcatc gcctttgcgg gcttccagtt ccacatgccg
     5401 cggcctgcgc cgtcgaaatg ccgtgaaggc agcgggcctc gagcttcgat ctccgcgagg
     5461 atttcgtcgt gcaggtgcgg gtgggtgcgc tcgacgtccc cgtaccagtt gcgccctccc
     5521 gcctgcatcg cggcacggca gagtggccag tcctccacgg ggagaagaca cgcctcgtgg
     5581 gcccagtact cgaagacgcg tccctggcgg agaagtcggt cgacgccgcc ccgggggtag
     5641 tcgccgacgc gggctccgag cacgatgcgg tggctgcgct cgacggtcga gatcgagtcg
     5701 agctgaacgc aggacagccg ccggatcgcc tcctcggtct ctcgcagcgt cccgcgccgc
     5761 gacctcgacg ccatgccctg tgcgttgacg gcgaggcgtc gcagcactgt gaccgtgacg
     5821 gtacgcacgg gccaacgcta gacgaccaca cgtcagctac cgtcgagcgc agtgcgccgc
     5881 cggatcctcg ccgcggttct gctcttcgtc gcgatggccg ttcccagcgc gtcgctcttc
     5941 gtcctcaggg acgacgacga gctgccggag cgtgccgacg cggtggtcgt gctcgccgga
     6001 tccgcgtctc gactcccggt tgccctcgac ctgatcgagc ggggagtcgc ccccgtgctc
     6061 gtcgtctcgg atccggaggg gcggcgggac gacgatcggc gcgacctctg tgcggcgggt
     6121 tcggacgagt acgagctcat ctgcgtgctc cccgatccct actcgacccg cggcgaggcg
     6181 cgcatggtcg cccgccttgc gtcggagcgc gggtgggagt cgatcgtcgt cgtcacctcc
     6241 cgcttccatc tcttccgtgc gaagcgcctc ttcgagcgct gcttcggcgg tgaggtcgct
     6301 ctcgtcggct ctcccgtcag ctggtggtac cggcccatcg ccgtcgcctc cgagtgggca
     6361 aagctcgcgg tcgccgagac gtttcgccgc ggctgctgac gcctctagtc ggggacgccc
     6421 ccctggagga tggcggcgag ccgagacgag tcgatgtttc cgccggagac gatgcagacg
     6481 actcgctgcg acggttcggc gtttgcgagc gccgctgcaa cgggcagcgc ggccgccccc
     6541 tcggcgacga cccggcctcg ctcggcaaga agccgaaccg ccgccgccgc cgcttccagc
     6601 gagacgacgt gcgcgccggc gatcaggtcc cgggcccgct cccacatcgg ctcgagcagc
     6661 gccttgctgc cggcgccgtc cacgaaggac ggccggtagt cgacggtcac cgggcgtcct
     6721 gcctcgagag ccgccgagac gggggcgccg gtctcgggct cacaggcgaa cactttcgta
     6781 cgggggctca gcgccgacag cgcgcttgcg atgccggtgg tcaggccgcc gccaccccac
     6841 ggaatcagca ccgtgtcgat ctcgccgagc tgttccgcga gctccaaccc gatcgttccg
     6901 ttgcccgcca tcacccgctc gtccatgacc ggatggacga agaagcccgg cgcgtcgtcg
     6961 aaacggctct cctcgagggc ctgccaccag cgctcgtacg gcaccttcac aatgtggccg
     7021 ccgagccgat cgatggcagc gagcttggct tcgggagcat gccccgggac gacgatcgtg
     7081 gccgggaggc ccagctcgcg cgccacccac gcgacgcctt gcgccatgtt tcccgcgcta
     7141 gccgtcacga cgcctcgctc cagcgccccg cgcggcgcac ggcgaatcgt gttcgtcgcc
     7201 ccccgcagct tgaaggagcc gatcggctgg aggttctcga gcttgagcca gatctccgcc
     7261 ggcgactcgg gggcatccag tcgaacgagg ggcgtgcgga cgactacgtc gcggatggtc
     7321 tcccgcgccg cggtgatgtc ggccagcggg atcagaacgg cacctcgacg cccaggctcc
     7381 tttcggccgc gcgggcgaga cacagctcgg cggccgcgag atcctcgacg gccagcccca
     7441 gcgacttgaa caccgtcagc tcctcgacac tcccccgccc ctcgacggcg ccggtgagga
     7501 gctctccgag ctcggcccgg atgtgctcgg ggccgatacc cgcctcctcg acagccatga
     7561 ggtagtcgcc ggcctcgttc agcgtcgact cgcgcctgtc gacgaacagc gctgccgcgg
     7621 agatggtctc ggcgtcgagc tcgcgcgcgg tcgggatgct ggccccgacg gcgttgacgt
     7681 gcgccccctc cgccagccat gcgcgacgca gaacgggctc gcgcgacgat gtgcacgtgc
     7741 aaacgacgtc cgcgcctgca accgcctcct cggccgagcc ggcgagcacg acattctcct
     7801 tcgcggcgag cctctccgcc cggcctcggc tccggctcca gacgcggacc tcgacgcccg
     7861 ggagcacggc gcgcatcgct tcgatgtgtg atctcccctg gacgcccgcg ccgagaatcg
     7921 caacactcgc ggcgtcggct cgcgcgagga ggcgcgtcgc tacggccgaa acggcggccg
     7981 tccggatctc cgtcactgcg gacgcgtcga gaacggcgcg aagctgtccc gttgcgccgt
     8041 cgtggacgag caccgcgccc tggtgggggt cgagcccgcg cgcggagttg ccgggcgcga
     8101 tgacgatctc cttgagcgtg aagagcggcc ggtcgcctcc gcgatgcgcc ggcatgaacc
     8161 ccatcagccg gctttccccg ggcgggcgca tgacgaatcg cagcggcatc gacagctctt
     8221 ctcgtgcgag tgccgcgagc gccccctcca tggcggagat gcaggacggc atgtcgagca
     8281 cggcccgaac gtcgtcgccg gagaggacga gaacgctcac gggaacagcg ccgtgagccg
     8341 gtcgcgctcg gccgccgaga gcgacgcgct ctgcgcctct ttgacggggg acaggtgctc
     8401 gacctttccg ggccccacga tgaccgccgt gatctccggc acggcgaggg cccacgcgag
     8461 cgcgagcccc gctatcgaga tgccgcgctc ggcggctgca cgctcgaatg cctcgagagc
     8521 gtcgaagatc gcgtcggccg cgtagccttc acttccctcc ggccgtagcg tcatgcgcga
     8581 gcccgcgggg aagggctcgc cgcggcggta cttgccggtc agccagcccc ccgcgagggg
     8641 gctgaacggc gtgaagccga ggccgtgctc gtggcagacg ggaaacaccg tctgcgcgtc
     8701 gccgagctcg agcagcgaga acgcgttctg cacccattcg aaacgcgcga tcccctcgag
     8761 acgtgagatc tccaccgcct cggcgaggtg ctcggccgag atgttcgagg ccccgatggc
     8821 acccaccttg cctgacgcga cgagtccgtc cagcgctccg agcgtttcct cgatcggcgt
     8881 ctcctcgtcc cagtcgtggg tcatgtagag gggaatgcgc tcgagtgcga gccgctcgag
     8941 gctcgtctcg acctggcgga cgatgcgcgc gcgcgaaaga ccgcggtcgg ccccttctgc
     9001 catggggttg aacgtcttgg tggtcacgat cgcgcggtcg cggacgtcgc ggctgcgtgt
     9061 cgccagccac tccccgatga acgactcgct gcgaccgcca ccgtacgcgt cggcggtgtc
     9121 gagcgtcgtg atccccagat cccacgcggc gtcgagaatg cgatgcgcct cgtcacggga
     9181 ggtgccctgt ccgaagaact gcggtgccga gccgatgcca ccgaagccgc cgcagccgag
     9241 gatgatccgt gagacggaga ttccgctttg gccgagctct cgcgcctcca tcgggcgatt
     9301 ctacggcggt gcgtccacgg cagccgggac gggaaccgcg ggacgtcgtc ggacgcggct
     9361 gagaggcagc aggccgagcg ccgcgacgca cgcggccacc acgaagagcc cgtcgactcc
     9421 ggccgttgtc tcgaccgcgc ccagtccgat cgctcccgcg gcgaagccga ggtcggcgaa
     9481 ggcggtgaag ctgccgacga cggcgcttcg ctcggcgatc gacgccgctg cgactgccag
     9541 cgtcatcaga gcggggaaga cgagtgcctg gccggcggcg aggacgacgg tccccgcgta
     9601 gagaccggcc ggcgaggagg agagggctat cgcgaggaag cccgacgcta cgagggcgag
     9661 ggcgagcgtc gcagtgcgct tcggcccgac ccggtcggga atctgtcgcc cgaagctgcg
     9721 gatagcgacg acgaccgcgg cgttgacggc gaaaacggtg cctgctccgg acatgccgat
     9781 gtcccgggca tagatcggca cgaaggcggc gaagccggcg aagccgacca tcgaggcagc
     9841 cagcgcgaga cccgggaagg tcgcggctcg actgaagagc cggccggcct tttcccgccc
     9901 gtcggtcgcg gggcgtgtct ccggcagggc gatgccgacc aggccgcctg cgagcgcgaa
     9961 cgcggcggcg gtcagccaga cggcgtcgaa gcggtcgtcg gcgagcacga gctcgccgag
    10021 gagcgggccg accgcgaggc cgccccagac ggcgagcgag tagagggaga gagcctcgcc
    10081 tcgtcgcttg tcgggcgcga ggtcgttcac caccgtcgcg gtgccgacga agatgagcgc
    10141 ctcgccgacg ccggtcacga ggcggagcag gacgagcagc ggaagcgact cggcgccggt
    10201 gtaggcggcg atcgacagcg cggcgatcac gccgccgccg acgatcagga tgcggcggcc
    10261 gcgtcggtcg ccgaagcgcc cgccgagcgg ctgaaagagg aatgcagtcg ggctcgccgc
    10321 cgcgacggcc aggccgatgc cgagtccgct cgcgcccagc ggccccttcg cgtagagggg
    10381 aaggacgggc aggagcgtcc cgatcgagag cagcagcaga aacgtcgccg cccagttgac
    10441 ggcgaagggt ctcgtcagca ggcgctcgga cggcacggcg cgatccaacc agacgtgagt
    10501 gcgccgcatc tgcggggcgc tcgtcagaca gacctgagag ctggcgcggt ttgactgcct
    10561 cttctaccgt cgccctgccg acgctcggcc ggccggaggc gtcctacgag gagcccgcca
    10621 cgtaggccgc caccgcctgg atctcctcgt cgctcaggag ctcgccgaag gcgggcatcg
    10681 ccccgctctc ctcgcgtacc tgaacctcga tctgctcggc ggtcagggac gtctcgtcga
    10741 ggttcgggcc gatcgaggcg gtcgagcctg ccgccgacaa ggtgtggcag ccgccgcagc
    10801 cgctcgacgc gaacagggcg gcgccgtcgg cggaggtgcc gccgttctcg ggctgtcccg
    10861 tctcggtctc cgccggtgga ggcggcgggc tcgcggactc gccgtcgccg ttcccgccgc
    10921 cgcaggccgc gaggacgagg aggaggagga ggacgaccgc aatcaggatt ccggcggcgc
    10981 gcaccggggc agtatccttc gcgccccgcc cgacgatggc tagatctcgg cggcgagcac
    11041 cttcgagcgc tcggtcagct cgtccacgtc gagctcgtcg cggacgcggc agtcgaagta
    11101 cacgtaggtc gtcccgatcg ccgccagcgg aatcaggaag gtgtagatga ctgccgcgat
    11161 gacgttgacg aaattgaagc tcgcatcggt gacgaggagc atcagcgcgc cgacgaaggg
    11221 gccggagacg agcgcgacgg tggtcatgac gatcgcgatc gaggcgacct tccaccagcg
    11281 cccggacacg agccgcgagc tgcggcggag cacgtgctcg cggctgtcgc cctcgagctc
    11341 gatcacctgg ccgatgaacg cccaccgtcc gaccagccag atcgcgaacg gaatgccgat
    11401 ggcggtgagg ttgaggatcc cgacgacgac aacgacgcgg acgagcgccc agatcagcgt
    11461 ccagaccttc ttgagcacac tgaggtagac gactcgcgcg gagacgggct cgccggcgtc
    11521 gatcgtcacc atcgcccgcg cggtcgcggc ctgcacgatc gtgaggccca ccag
//
LOCUS       DBNGNK010000127         6132 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1238897,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000127 DBNGNK010000000
VERSION     DBNGNK010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1238897"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..595)
                     /gene="rimM"
                     /locus_tag="AC150V_03700"
     CDS             complement(122..595)
                     /gene="rimM"
                     /locus_tag="AC150V_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR3762056.1"
                     /translation="MPDELVPVGQVGRPHGLDGSFVVERSSDDPGRFAVGATLLVDGE
                     ETAVVSSRRVGGGRIAIRLDRPADRGATLSVRRADLPEPQDGHYYAFQLLGLRVIDRS
                     GRAVGVVDEVVPGPANDNLALDTGTLVPLIEDAVAEIDLARGQIVVDGDFLGLAD"
     gene            complement(606..839)
                     /locus_tag="AC150V_03705"
     CDS             complement(606..839)
                     /locus_tag="AC150V_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005551913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR3762057.1"
                     /translation="MAELIAWLAQQLVDDPDAVRVEEEQRDDAVVLRLHVAPDDVGKV
                     IGRQGRIARALRTVARAGGVRAPHRVVLEIAED"
     gene            complement(855..1097)
                     /gene="rpsP"
                     /locus_tag="AC150V_03710"
     CDS             complement(855..1097)
                     /gene="rpsP"
                     /locus_tag="AC150V_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015554336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR3762058.1"
                     /translation="MAVRIRLTRVGSKKNPIYRVVVADKRSPRDGRFIEIVGRYNPQT
                     DPSTIDLDEEKIKDWIAKGAQPSDTVARLIKAAGIE"
     gene            complement(1170..2501)
                     /gene="ffh"
                     /locus_tag="AC150V_03715"
     CDS             complement(1170..2501)
                     /gene="ffh"
                     /locus_tag="AC150V_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR3762059.1"
                     /translation="MFDSLSERLQGTLGDLRGKRRLDEETVSKAMREIRLALLEADVN
                     FQVVKDFVFSVKARALGEDVLAGLDAGQQVVKIVHDELTELMGTGDSRLVFGKPPTVV
                     LMAGLQGSGKTTASAKLALHLRKHDKKTPGLVAADLQRPAAIDQLEQLGRQIQVPVYR
                     TDTQDPVEAAERGLERAAEDGCDVVIVDTAGRLNVDEELMDELVRVQEATKPLNVLLV
                     LDAMTGQEAVNVAEAFHQRVAFDGVLLTKLDGDARGGAALSVKAITGKPVKYASVGEK
                     LDQLEVFHPDRMASRILGMGDVLTLIEKAEQAVEEDEQAEMERRLLAGEFTFDDFLSS
                     YRMLRRMGPLKGVMKLIPGVGQQLDALDIDERQMARVEAIVLSMTPHERRVPHVINGP
                     RRRRIADGSGTSIDEVNKLLQARKQMQKMMKQMGKGKMPGLPPELTKAGKR"
     gene            complement(2534..3493)
                     /locus_tag="AC150V_03720"
     CDS             complement(2534..3493)
                     /locus_tag="AC150V_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3762060.1"
                     /translation="MRLPAVVRRAAQNRRLVILLSTGLVLIASALTARYFLQIGWPLH
                     RADALLVGVAGGLFLVAHSARTLGWGRTFAVAERPGLLALAAASGAASLMSVALPGRL
                     DTVVRIAVVRRFPGGAKGFGAVCLSIVVLGMIEAAALTPLAGFAAGAGGSPTWLRAGL
                     IVVAAAGAGSLAALFALPHLTGAKALARCRAVGWLSRHSTCPREATKALGLVSVSWLC
                     RAAALLVLLEALGLQASVPLALVLLCASAAASIVPIAPAGAAVQAGAGAAVLAASGVA
                     TPTAVAFGLAAQILLVLAGGVLGIGAALWHGGLHVVALRQRTA"
     gene            complement(3614..4564)
                     /gene="ftsY"
                     /locus_tag="AC150V_03725"
     CDS             complement(3614..4564)
                     /gene="ftsY"
                     /locus_tag="AC150V_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR3762061.1"
                     /translation="MSRTWSELLGDDERAESDEDERTGFFSRLRDSLSKSRRALTEEL
                     ALGTFDPDDEEAWERLEIALLQADVGVPATAELVRRLEERGEVSDLGEALVPELARMF
                     GEPPSLDLSATPSVLLVVGVNGTGKTTTIGKLAQKLREHDRSVLLGAADTFRAAAEEQ
                     LEIWAERAGAEFVGAPSGGDPAAVAFDAVEAGKARGKDVVIVDTAGRLHTQSNLMEEL
                     AKVRRVIASRMEGAPHETLLVVDATTGQNGLQQARLFGEAAGVTGVALTKLDGSAKGG
                     VAVAIAYELELPVKLVGIGERLDDLRPFDADDFARALVSG"
     gene            complement(4561..>6132)
                     /locus_tag="AC150V_03730"
     CDS             complement(4561..>6132)
                     /locus_tag="AC150V_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3762062.1"
                     /translation="DARRRELADEAAELAARATTAAATAADAAVRATEAEAAFKEVAH
                     LRECVLDEALLDRLARCSGAVDESLQRAAEIVARVEAPLAARVNASSRRTGELGAELQ
                     RLATLESDARRDASEASARAHAAEVVAVRLGGSVEGALVDPAVDLEELAREAAELLTA
                     AESSANEAREAADRARSAGAALAERTPRRSTLDTDLLGRLVAMAASLGTGVEQAAARA
                     ERLQAPVRARVAAGARRAADLGTELRRLGAAEVELRQSSEEAAERATAVEVEIARLEA
                     EAEEARRRLQEAGAVEPAGGDVRDELAIRVERLESRRETLGRVNPLAKEEYEAEKERL
                     SELTTQREDLEESLAELSKLRDELAETVRRRFAETFDAVATNFEEVAATLFPGGEGRL
                     RLVEPDDEEDAEAGVEIELRPAGKRITRLSLLSGGEKALGAISFLFALFLAKPCPFYL
                     LDEVEAALDDTNITRFVELLRRYAEQAQFVVITHQKRTMEAADILYGVTMGADGVSQV
                     VSRRLPREHASAVGA"
BASE COUNT          909 a         2227 c         2068 g          928 t
ORIGIN      
        1 gcgagcggtt tctgctccgt cagccctgcg aaggcgtgcg gaacctgcgt gaagacgtcg
       61 acccgcaagc cgaccaacct acccgagccg agcggctgac cgggaatcgt cgtccatccg
      121 gtcagtcggc cagcccgagg aaatcaccgt cgacgacgat ctgtccccgc gcgagatcga
      181 tctccgcgac ggcgtcctcg atcagcggca ccagcgtccc tgtatcgagc gcgaggttgt
      241 cgttggcggg tccgggcacc acctcgtcga cgaccccgac cgcccgtcct gaccggtcga
      301 tcacgcgcag gcccaggagc tggaaggcgt agtagtggcc atcttggggc tcggggagat
      361 cggcccgcct gacggagagt gtcgctcccc tgtcggccgg ccgatcgagg cggattgcga
      421 tgcgtccacc gcccacccgg cgagaggaga cgacggccgt ctcctcgccg tcgacgagca
      481 gcgtggcccc gacggcgaag cgcccaggat cgtccgagga ccgctcgacg acgaaggagc
      541 cgtcgagccc atgcggcctc ccgacctgac cgacggggac gagctcgtcc ggcatgcgcc
      601 cggactcagt cctcggcgat ctcgaggacg acacgatgcg gcgccctgac gccgcctgcg
      661 cgcgcgacgg tgcgcaatgc gcgcgcgatc cttccctgac ggccgatcac cttgcccacg
      721 tcgtcgggtg cgacgtggag tcgcagcacg accgcgtcgt cgcgctgctc ctcctcgacc
      781 cggacggcat ccggatcgtc cacgagctgc tgggcgagcc acgcgatcag ctcggctatc
      841 ggcattacgt cgtgctactc aatacccgcg gccttgatga ggcgggcgac cgtatcgctc
      901 ggttgggcgc ctttggcgat ccagtccttg atcttctcct cgtcgaggtc gatcgtcgac
      961 ggatcggtct gcgggttgta gcggccgacg atctcgatga agcgcccatc ccgcggcgag
     1021 cgcttgtcgg cgacgacgac gcggtagatc gggttcttct tcgagccgac gcgcgtcagc
     1081 ctgatgcgaa ctgccatgaa gtgtcaatcc tctttctctc gctggtggtc agcggcgtat
     1141 tgtgcctggc cctgctccga gccgcccgtt cagcgctttc ccgccttcgt gagctcgggc
     1201 gggagaccag gcatcttgcc cttccccatc tgcttcatca tcttctgcat ctgcttgcgc
     1261 gcctgcagca gcttgttcac ctcgtcgatc gacgtcccgc tgccgtcggc gatcctccgg
     1321 cgccgcggcc cgttgatgac atgcggcacg cgacgctcgt gcggcgtcat cgagagcacg
     1381 atcgcctcga cgcgtgccat ctgccgctcg tcgatatcga gcgcgtccag ctgctgtccg
     1441 acaccgggaa tgagcttcat cacgcctttg agcggtccca tccgacgcag catccggtag
     1501 ctggacagga aatcgtcgaa ggtgaactcc ccggcgagca gccggcgttc catctcggcc
     1561 tgctcgtcct cctcgacggc ctgctccgcc ttctcgatca gcgtcaggac gtcgcccata
     1621 ccgagaatcc gcgacgccat cctgtcgggg tggaagacct cgagctggtc gagcttctcc
     1681 ccgaccgagg cgtacttcac gggcttcccg gtgatcgcct tcaccgagag cgcggcgccg
     1741 ccacgggcgt caccgtcgag cttcgtcaag agcaccccgt cgaacgcgac ccgctggtgg
     1801 aacgcctcgg cgacgttcac cgcctcttgg ccggtcatcg cgtcgagcac gaggagaacg
     1861 ttcaacggct tcgtggcctc ctggacgcga acgagctcgt ccatcagctc ctcgtccacg
     1921 ttgaggcggc cggcggtatc gacgatgacc acgtcgcatc cgtcctcggc ggcccgctcg
     1981 agccctcgct ccgcggcctc gaccgggtct tgcgtgtcag tccgatacac gggaacctgg
     2041 atctggcggc ccaactgctc gagctggtcg atcgccgccg gccgctggag atcagctgcc
     2101 acgagaccgg gcgtcttctt gtcgtgcttg cgcaggtgga gagccagctt cgcgctcgcc
     2161 gtcgtcttgc cggagccctg cagccccgcc atcagcacga ccgtcggcgg cttgccgaag
     2221 acgagccgcg agtcgcccgt tcccatcagc tcggtgagct cgtcgtggac gatcttcacg
     2281 acctgctgtc cggcgtcgag cccggccagc acgtcctcgc cgagggctcg cgccttgacg
     2341 gagaagacga agtccttgac gacctggaag ttgacgtcgg cctcgagcag cgccagccga
     2401 atttcgcgca tcgccttcga gaccgtctcc tcgtcgagcc tgcgtttccc tcgcaggtcg
     2461 ccgagggtcc cctgaaggcg ctctgacaac gagtcgaaca cggtcaggcc agtctagagg
     2521 gcgccgctga gtctcaggcc gtgcgctgcc gcagcgccac gacgtgcaac ccgccgtgcc
     2581 agagcgcagc gccgatgccg agcacgccgc ccgcgagcac gagcaggatt tgcgcggcga
     2641 gaccgaaggc cacagccgtg ggggtcgcga cgcccgatgc tgccagcacg gcggctccgg
     2701 cgccggcctg aaccgcggcc ccggccgggg cgatcgggac gatcgaagct gcggccgacg
     2761 cgcacagaag gacgagtgcg aggggtaccg aggcttgaag tccgagcgcc tcgagcagga
     2821 cgagcagcgc agctgcgcgg cagagccagg acacgctgac gagcccgagc gctttcgtcg
     2881 cctcgcgcgg acacgtcgag tggcgcgaga gccagccgac ggcgcgacac cgggcgagcg
     2941 ccttcgcacc ggtcagatgt ggcagggcga agagagcggc cagcgagccc gcgcccgcag
     3001 cggcgacgac gatcaagccg gcccggagcc acgtcggcga cccgccggcg cccgccgcga
     3061 atcccgccag cggggtgagg gccgcagctt cgatcatccc gagcaccacg atcgacaggc
     3121 agacggcacc gaagcctttc gccccgcccg ggaagcgcct gacgacggcg atccgaacga
     3181 cggtgtcgag cctgccgggc agcgcaacgg acatcagcga tgctgctccc gaggcggccg
     3241 ccagagccaa gagcccgggc cgctcagcga cggcgaatgt ccggccccag ccgagcgttc
     3301 gtgccgagtg cgcaaccagg aagagacctc ccgcgacacc cacgagcagc gcgtccgcgc
     3361 gatgcagcgg ccagccgatc tggaggaagt aacgcgcggt cagcgccgac gcgatcagca
     3421 cgaggccggt cgacaacagg attacgaggc gccggttctg agccgcacgc cttaccaccg
     3481 caggtagccg caccggctga ttgtcggcgc gagcccctcg acgacgcatc acccgaaaag
     3541 gtaggtacga ccccaagacc tacatggtgc ctgaggcccg cgcgacgcac gcggagaacc
     3601 cgttacgcaa ttgttagccg gagacgagcg cgcgggcgaa gtcgtcggcg tcgaacgggc
     3661 ggagatcgtc gagccgttcg ccgatcccga cgagcttgac cggtagctcc agctcgtagg
     3721 cgatggcgac cgcgacgccc cccttggcgg agccgtcgag cttcgtcagg gcgaccccgg
     3781 tgacgccggc ggcttcgccg aagaggcgcg cctgctgcag accgttctga cctgtcgtcg
     3841 cgtcgacgac gagcagcgtc tcgtgtgggg cgccctccat gcgggaggcg atgacgcggc
     3901 gtaccttcgc gagctcttcc atcaggttgg actgtgtgtg taggcgcccc gcggtgtcga
     3961 cgatgacgac gtccttgccg cgcgccttgc cggcctcgac cgcgtcgaag gcaaccgctg
     4021 cgggatcacc gccgctcggc gccccgacga actcggctcc ggcacgctct gcccagatct
     4081 cgagctgctc ctccgctgcg gcgcggaacg tgtcggcggc gccgagcagg acgctgcggt
     4141 cgtgctcgcg aagcttctgc gcgagcttcc cgatcgtcgt cgtcttgccg gtgccgttca
     4201 cgcctacgac gaggagcacg ctcggggtcg cggagaggtc gagagacggg ggctcaccga
     4261 acatgcgcgc caactccggc acgagcgcct cgccgagatc tgagacctcg cccctctcct
     4321 cgagccgccg gacgagctcg gcggtggccg gcacaccgac gtctgcctgg agcaacgcga
     4381 tctcgagccg ttcccaggcc tcttcgtcgt cggggtcgaa cgtgccgagc gcgagctcct
     4441 cggtgagcgc acgccggctt ttcgagagcg agtcacggag gcgcgagaag aagcccgtgc
     4501 gctcgtcttc gtcgctctcg gcccgctcgt cgtcgccgag cagctccgac caggtgcgac
     4561 tcacgcgccg acggcgctcg cgtgctcgcg cggcaggcgc cgggacacga cctgtgacac
     4621 tccgtcggct cccatcgtca cgccgtagag gatatcggcg gcttccatcg tcctcttctg
     4681 gtgggtgatg acgacgaact gggcctgctc ggcgtaacga cgaagcagct cgacgaagcg
     4741 tgtgatgttc gtgtcgtcga gggccgcctc gacctcgtcg aggaggtaga aggggcacgg
     4801 cttcgcgaga aacagcgcaa agaggaagga gatcgccccg agcgccttct cgccgcccga
     4861 cagcagcgac agccgggtga ttcgcttgcc ggccggtcgg agctcgatct cgacgcccgc
     4921 ctcggcgtcc tcctcgtcgt caggctcgac gaggcgtagt cgcccttctc cgccgggaaa
     4981 gagcgtcgcg gcgacctcct cgaagttggt cgccacggcg tcgaaggtct ctgcaaagcg
     5041 tcgccgcacc gtctcggcga gctcgtccct tagcttcgag agctccgcaa ggctctcttc
     5101 caagtcctcg cgctgcgtcg tcaactcgga gagtcgctcc ttctcggcct catactcctc
     5161 cttcgcgagc ggattcacgc ggccgagcgt ctcgcggcgc gactcgagcc gctcgacgcg
     5221 gatggcaagc tcgtcgcgga cgtcgccgcc cgcaggctcg acggcccccg cttcctgcag
     5281 ccggcggcgg gcctcttcgg cctccgcctc gagccgggcg atctccacct cgaccgcggt
     5341 ggcccgctcc gcggcctctt cgctcgactg ccgcagctcg acctctgcgg cgccgagccg
     5401 tcgcagctcc gtgccgagat ccgccgcgcg ccgggcgccg gcggcgacgc gcgcacggac
     5461 gggtgcctgc agccgttccg ccctcgccgc ggcctgctcg acgcccgtgc cgagcgaggc
     5521 ggccatcgcg acgaggcggc cgagcaggtc ggtatcgaga gtggagcgcc ggggggtgcg
     5581 ctccgccagt gccgccccgg ccgagcgagc ccgatctgct gcttcgcggg cctcgttggc
     5641 ggaggactcg gccgccgtga gaagctcagc tgcctctcgg gccagctcct cgaggtctac
     5701 ggcgggatcg acgagcgctc cctcgaccga gccgccgaga cgcaccgcga ccacctcggc
     5761 ggcatgagcg cgagcggacg cctcggaggc gtcgcgccgc gcgtcgctct ccaacgtggc
     5821 cagccgctgc agctcggcac cgagctcgcc ggttcgccgc gagctggcgt tgacgcgggc
     5881 ggccagcggt gcctcgacgc gcgcaacgat ctcggctgcg cgctgcagcg actcgtccac
     5941 ggcgccggag cagcgcgcaa ggcgatcgag cagtgcttcg tcgaggacgc actcccgcag
     6001 gtgcgcaacc tccttgaagg cggcctcggc ttcggttgcg cggaccgcgg catccgccgc
     6061 cgtcgcggcg gccgtcgtcg cgcgggctgc gagctcggcc gcctcgtcgg cgagctcacg
     6121 gcggcgcgca tc
//
LOCUS       DBNGNK010000128         4451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_307751,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000128 DBNGNK010000000
VERSION     DBNGNK010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_307751"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="AC150V_03735"
     CDS             complement(<1..431)
                     /locus_tag="AC150V_03735"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014442855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR3762063.1"
                     /translation="MEAHSGRGVAIGVAIAFAFLLGLLPGAPTDLARGAVPAGFQETA
                     ALTGLTNPTVIRFASDGRVFVAEKRGVIKVFDDLADTTPTVFADLNVNVHNFWDRGLL
                     GMALAPNFPADPWVYVLYTYDAAPGGVAPRWGSPGVYSDPCP"
     gene            complement(520..1305)
                     /locus_tag="AC150V_03740"
     CDS             complement(520..1305)
                     /locus_tag="AC150V_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3762064.1"
                     /translation="MTALRVFFIGGRISYHALFNWISPLLYITTMLGSPLFQILFFTY
                     LGRFAQSQDDSFFIVGNAVQVSAMAGIYGMTMGIANERQFGTLQSLLATPANRLAMFA
                     GRALPFILNGMIVSAFGFAVGWILLDFRPAEGSLLPLAFIVLVTTCSCTCLGMLIGSI
                     GLRARDVFFGANLVYFLMLLVCGVNVALEDMPGWMAAIGRSLPLTHGIEAARDVAAGA
                     SLSEVSGLIATEAAIGLAYATVAFVLFRVFEHEGRRRGSLETY"
     gene            complement(1302..2078)
                     /locus_tag="AC150V_03745"
     CDS             complement(1302..2078)
                     /locus_tag="AC150V_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3762065.1"
                     /translation="MRSGLRVVWVGWLFHLKSLTNSLFFVLVSIGQPIIFASIAFFMV
                     GDGSSGEALLYVALGAGLMGIWSATLFGSGGAIQWQRWQGTLELLVAAPTPFIMSLLP
                     LTIATSSIGLYSVVATLVWGRVFFGVPLDFAEPLQFVVALPVTVLSLGMLGLVLASTF
                     VLVRHASAFSNLLEYPVWLATGLLVPLALLPGWVEPLSWVLAPSWGITAIRDAALGTG
                     TAWPAVGMCVLLGAIYLALGAVFLRNFERMARQRATLSLT"
     gene            complement(2075..3043)
                     /locus_tag="AC150V_03750"
     CDS             complement(2075..3043)
                     /locus_tag="AC150V_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762066.1"
                     /translation="MPVVEAVDLYRTYRTTTGSVRRRSMEVEAVRGVSFEIADGELFG
                     LLGPNGAGKTTTIKMLITLLLPTAGTARVLGHDVVKDARWVRRQIGYVFGGDRGLYER
                     LSAQDNLRYFAELYGVEPQIQRTRIDELLELVQLKGREKERVEGYSRGMRQRLHIARG
                     LLHDPPVVFLDEPTIGVDPVGARELRAMISGLTDAGKTVLLTTHYMFEADALCDRIAV
                     IANGKIVAHGSPRELKRLVADGSVVEIEVYGIDAETVEGLRSLDGVTAVSVEDREQAQ
                     VVIVQSAAGHELAHAVLRRLGDVNVGRVSAREPTLEDAYVALVTEQ"
     gene            3198..4388
                     /locus_tag="AC150V_03755"
     CDS             3198..4388
                     /locus_tag="AC150V_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3762067.1"
                     /translation="MSKRLTLVGALVTLAALVAAAFATTAGAGSSATQQGVSCKSNLK
                     LGLVTPLTGGAGFLGQDQLSWARYAAKTLGPGLGLKVRIVNGDTPVEQGVGIAQTVAQ
                     KFIADKQIVGIIGPATSGASAATSKAFGDANMPHVSPSATRTSLTKGANVEATNRFFR
                     VVPGDYIQGPTVARFMADTLNAQKVVLVDFQEPYSQGLTQAADVVLKKNNVSTVKLSI
                     PNTVTDFSSFVTRVPTDADIVFFVTQKPADAQTFSRQLIEQGRKAIVFGPDGANDPDA
                     FKTPGAYVSNFAPDISAFARNKELIDGWKKDNPQATLGSFGPPAYLATQVVLLAIKRA
                     CNAKNGTITRLDVQKRIKRVRVPNSILGGSFRFSTKSNDPLNAKFYIFQIQQNGTYKL
                     VETS"
BASE COUNT          812 a         1529 c         1461 g          649 t
ORIGIN      
        1 gggcacgggt cgctgtacac gccgggcgag ccccagcgcg gggccacgcc gcccggcgcg
       61 gcgtcgtacg tgtacagcac gtagacccac gggtcggcgg ggaagttcgg cgcgagagcc
      121 atcccgagga ggccgcggtc ccagaagttg tgaacgttga cgttgaggtc cgcgaagacc
      181 gtcggagtcg tgtccgcgag gtcgtcgaac actttgatga caccgcgctt ctcggcgacg
      241 aagacgcgtc cgtcgctcgc gaaccggatc accgttgggt tcgtcaggcc ggtgagggct
      301 gcggtctctt ggaagccggc tggaaccgct ccgcgcgcga ggtcggtggg cgcaccaggg
      361 agcaagccga gcaggaacgc aaaagcgatc gcaacgccta tggcgacccc gcgcccgctg
      421 tgagcctcca ccctttcccc tttgctgttc gtgcgccttc aagtatccgc ctcaacggca
      481 ctcgtgtcga attccgggcg ggtggtcttt tgagtcgggt cagtacgtct cgagcgagcc
      541 gcgccggcgc ccctcgtgct cgaacactcg gaagaggacg aaggccacgg tggcgtaggc
      601 gagtccgatc gctgcctccg tcgcgatcag gccggagacc tcactgagcg aagcacccgc
      661 ggcgacgtcg cgtgccgcct cgatcccgtg cgtgagcggc agactgcggc cgatcgcggc
      721 catccagccg ggcatgtcct cgagcgccac gttcacgccg cagacgagca gcatcaggaa
      781 gtagacgagg ttcgctccga agaagacgtc gcgcgcgcgg agtccgatcg agccgatcag
      841 catgccgaga cacgtgcacg agcaggtcgt gacgagcacg atgaaggcaa gaggcagaag
      901 acttccctcg gccggtcgga agtcgagcaa gatccagccg actgcgaagc cgaacgccga
      961 cacgatcatc ccgttgagga tgaagggcag agcgcgccct gcgaacatcg ccagcctgtt
     1021 cgcgggcgtg gcgagcaggg actgaagcgt tccgaactgg cgctcgttgg cgatgcccat
     1081 cgtcatgccg tagatgcccg ccatggccga cacctgaacg gcgttcccga cgatgaagaa
     1141 gctgtcgtcc tgggactgcg cgaaccgtcc gagatacgtg aagaagagga tctgaaagag
     1201 cgggctgccc agcatcgtcg tgatgtagag cagcgggctg atccagttga agagagcgtg
     1261 gtaggagatc cgcccgccga tgaagaacac gcgcagcgcg gtcatgtcag cgacagcgtg
     1321 gcgcgctgcc gcgccatgcg ctcgaagttg cggaggaaca ccgcgccgag agcgaggtag
     1381 atcgccccga gcagaacgca catcccgacc gccggccagg cggtgccggt gccgagcgcg
     1441 gcgtcgcgga tcgccgtgat gccccacgac ggcgcgagca cccaggacag cggctccacc
     1501 cagcccggaa gcagggccaa tgggacgagc agtccggtcg ccagccagac cgggtactcg
     1561 agcaggttcg agaatgcgct cgcgtgccgg acgagcacga aggtcgacgc gagcacgagc
     1621 ccgagcatcc cgaggctgag caccgtcacc ggcagtgcca cgacgaactg cagcggctcc
     1681 gcgaagtcga gcggaacccc gaagaagacg cggccccaga ccagcgtggc cacgaccgag
     1741 tagaggccga tcgacgaggt cgcgatcgtc aggggcagga gcgacatgat gaacggcgtg
     1801 ggagccgcga ccagcagctc gagagtcccc tgccaccgct gccactggat ggcgcctccg
     1861 gagccgaaga gcgtcgccga ccagattccc atcaatcccg ccccgagcgc gacgtagagc
     1921 agagcctccc ccgagctgcc gtcgccgacc atgaagaatg cgatcgaggc gaagatgatc
     1981 ggctggccga tcgagacgag cacgaagaac agcgagttgg tgaggctctt cagatggaac
     2041 agccagccga cccagacgac gcggagccca ctcctcactg ctcggtcacc aacgccacgt
     2101 aggcatcctc gagcgtcggc tcgcgggccg agacgcgtcc gacgttaacg tcaccgagcc
     2161 gccgcagcac ggcgtgagcg agctcgtgac ccgccgccga ctggacgatc acgacctgcg
     2221 cctgctcgcg atcctccacc gagaccgccg tcacgccgtc caacgagcgc agcccttcca
     2281 ccgtctctgc gtcgatcccg tagacctcga tctcgacgac gctgccgtct gccacgagcc
     2341 gcttgagctc gcggggcgac ccgtgcgcga cgatcttccc gttcgcgatc accgcgatcc
     2401 tgtcgcacag cgcgtcggcc tcgaacatgt agtgcgtggt gagcaggacg gtcttgcccg
     2461 catcggtgag gcccgagatc atcgcgcgaa gctcgcgcgc ccccaccggg tcgacgccga
     2521 tcgtcggctc gtcgaggaag acgaccggcg ggtcgtggag caggccgcgg gcgatatgga
     2581 gccgttgccg catgccgcgc gagtagccct cgacgcgctc cttctcgcgc cccttgagct
     2641 gcacgagctc gagcaactcg tcgatccgag tccgctggat ctgcggctcg acgccgtaga
     2701 gctccgcgaa gtagcgcagg ttgtcctggg ccgagagccg ttcgtacagg ccgcggtcgc
     2761 ccccgaacac gtagccgatc tgcctgcgca cccagcgggc gtccttcacc acgtcgtgtc
     2821 cgagcacgcg agccgtcccg gcggtgggga ggagcagcgt gatcagcatc ttgatcgtcg
     2881 tggtcttgcc cgctccgttc gggcccagca gtccgaacag ctcaccgtct gcgatctcga
     2941 agctgacccc tctgacagcc tcgacctcca tggagcggcg ccgcaccgag cccgtcgtcg
     3001 tgcggtacgt ccggtacaga tcgacggcct cgacgacggg catcaggctc gccatcctag
     3061 ccctcgcctc gggaacgccc ggttccccgg tttgacgcct ttcccggcgc ctgccacagt
     3121 tccccggtgg cggcagccac aatcgtttct ctgcaccaga cgtctatatc gggaaacccc
     3181 agaaggaggg cacgtccatg tctaaacgcc tcacgctcgt cggagcgttg gtcactctcg
     3241 cggctcttgt cgcggcggcc ttcgcgacga cggcaggagc gggctcgagc gcgacccagc
     3301 aaggggtcag ctgcaagagc aatctgaagc tcggactcgt cacgccgctc accggcggcg
     3361 cgggcttcct cggccaggat cagctcagct gggcgaggta tgcggcgaag acgctcggcc
     3421 ccgggctcgg cctcaaggtc aggatcgtca acggcgacac gccggtcgag cagggcgtcg
     3481 gcatcgccca gaccgtcgcg cagaagttca tcgccgacaa gcagatcgtg ggcatcatcg
     3541 gcccggcgac gtcgggcgcg tccgccgcga cgagcaaggc cttcggtgac gcgaacatgc
     3601 ctcacgtctc gccgtcggcg acgcggacat cgctcacgaa gggcgcgaac gtcgaggcga
     3661 cgaaccggtt cttccgcgtc gtgcccggcg actacatcca gggcccgacg gtcgcccgct
     3721 tcatggccga cacgctgaac gcccagaagg tcgtcctcgt cgacttccag gagccgtact
     3781 cgcagggtct cacccaggct gcggacgtcg tgctgaagaa gaacaacgtc agcacggtca
     3841 agctctcgat ccccaacacc gtgaccgact tctcgtcgtt cgtcacgcgg gtgccgacgg
     3901 acgccgacat cgtgttcttc gtcacgcaga agccggctga tgcgcagacc ttctcccggc
     3961 agctcatcga gcagggtcgc aaggcgatcg tcttcggacc ggacggcgcg aacgaccccg
     4021 acgccttcaa gaccccgggc gcgtacgtgt cgaacttcgc tcccgacatc tccgccttcg
     4081 cgcggaacaa ggagctcatc gacggctgga agaaggacaa tccgcaggcg acgctcggct
     4141 cgttcggccc gcccgcctac ctcgcgacgc aggtcgttct gctcgcgatc aagcgtgcgt
     4201 gcaacgccaa gaacggcacg atcacccgcc tcgacgtgca gaagcggatc aagcgcgtgc
     4261 gggttccgaa ctcgatcctc ggcgggagct tccgcttctc cacgaagtcg aacgacccgc
     4321 tgaacgcgaa gttctacatc ttccagatcc agcagaacgg gacgtacaag ctcgtcgaga
     4381 ccagctagag ctggcacatt gcgtcttcgt gtgagcggcc cggcctctgc gccgggccgc
     4441 tcgccgttgt t
//
LOCUS       DBNGNK010000129         3288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_522534,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000129 DBNGNK010000000
VERSION     DBNGNK010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_522534"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC150V_03760"
     CDS             <1..530
                     /locus_tag="AC150V_03760"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3762068.1"
                     /translation="AGHVVDGRAEAALLEVARRERATLVAIGAHRRSRAAELLLGSTA
                     LAVIRDASCSVLAARETDDSGRFPRTIVVGLDGSAEAQAAAEVASNVSKHCGGAITGV
                     VATGGKDVDPEAATGQAFEVEVEADSPVDVLKRRGETADLLVVGSRGLHGVRALGSVS
                     ERVANGASCSVLVVR"
     gene            complement(543..1238)
                     /locus_tag="AC150V_03765"
     CDS             complement(543..1238)
                     /locus_tag="AC150V_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3762069.1"
                     /translation="MLEQPPLLTIRRGFERPAPELIGRIGAGETAQVADARAGRGALD
                     HRIRPVLELAPGTRIVGPALTCETGPADVLGALAALDVAQPGDVLLIATGGWAGTASI
                     GDQFAGAARNAGLAAIVTDGLVRDVDGLERAGVPIFSAGLSPNSPAATGPGTVGMPVQ
                     IGGLLVDAGDIVLCDRTGVVVVPRVEAEATAAAVERVVELEAAEAERVAAGTVVPDAI
                     RELLGSDRVREVD"
     gene            complement(1243..2043)
                     /locus_tag="AC150V_03770"
     CDS             complement(1243..2043)
                     /locus_tag="AC150V_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3762070.1"
                     /translation="MSDRLRVRKTYKLFLGGAFPRSESGRTFEADGQNVARASRKDVR
                     DAVRAARGAFPKWAGATAYNRGQVLYRLAEMMEARAADLAAACSGRDEVEESIDRVIW
                     YAGWADKLAQVLGSSNPVAGPYFNFTVPEPTGVVAIIAPDEPALGGLVARLAPALVGG
                     NALVAVASETHPIAAIELAEAIATSDVPAGVVNVLTGFRDELAPVLAAHMDVNSIDVT
                     GAGGEVAELERLAADNVKRVVRADPAEQSPWQIGAFLELKTVWHPAGL"
     gene            complement(2040..>3288)
                     /locus_tag="AC150V_03775"
     CDS             complement(2040..>3288)
                     /locus_tag="AC150V_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3762071.1"
                     /translation="VGAARSAYENGWSELRPAERAKYLFRIARILQERARELAVAESL
                     DGGKPIRESRDVDLPLAAAHFFYYAGWADKLEYAFPNRRPRPLGVAAQIIPWNFPLLM
                     LAWKVAPALACGNTVVLKPAETTPLTALLFADVCRQAGLPPGVVNIVTGDGSTGAHLV
                     RHDGIDKVAFTGSTEVGKAIRRELAGREIALTLELGGKAANIVFDDCALDQAIEGIVN
                     GIYFNQGHVCCAGSRLLVQESIYEPVVKKLKRRLGTLRVGDPLDKNTDVGAINSRQQL
                     EKIQELVASGEEEGAEVYQPPCRLPEKGYWFAPTVFTNVAQSYRIAQEEIFGPVLSVL
                     TFRTPEEALEKANNTAYGLSAGVWTEKGSRILWMAERLRAGVVWANTFNRFDPASPFG
                     GYKESGFGREGGRHGLEPYLRFE"
BASE COUNT          466 a         1159 c         1181 g          482 t
ORIGIN      
        1 cggccggaca cgtcgtcgac ggacgggccg aggcggccct gctcgaggtc gcgaggcgcg
       61 agcgggcgac gctggttgcg atcggcgccc accggcgctc ccgcgctgcg gagctcctgc
      121 tcggctcgac cgcgctcgcg gtgatccgcg acgcttcgtg ctcggtgctc gcggcaaggg
      181 aaaccgacga ctccgggcgt tttccgcgca cgatcgtcgt cgggctggac ggctcggccg
      241 aagctcaggc ggccgccgag gtggcttcga acgtgagcaa gcactgcggc ggcgcgatca
      301 cgggcgtagt cgccacaggc ggcaaggacg tggatcccga ggcggcgacg ggccaggcgt
      361 tcgaagtcga ggtcgaagcc gacagtcccg tcgacgtcct caagcggcgc ggcgagacgg
      421 ccgacctgct cgtcgttggc tcccgcggcc tccacggtgt gcgggcgctc ggcagcgtaa
      481 gcgagcgagt cgcgaacggc gcgtcgtgct ccgtgctcgt cgtccgctag cggccggcga
      541 gctcagtcga cctcgcgcac gcgatccgag ccaagcagct cacgaatagc gtccggcacg
      601 acggttccgg cggcgacgcg ctccgcctct gccgcctcga gctccacgac gcgctcgacg
      661 gccgccgcgg tcgcctccgc ctctactcga ggcacgacga cgacaccggt tcggtcgcag
      721 agcacgatgt cgccggcgtc gacgagcagg cccccgatct gaacgggcat gccgaccgtc
      781 ccggggccgg tcgcggcggg ggagttcggg ctcaaacccg cgctgaagat cgggacgcct
      841 gcgcgctcca gcccgtcgac gtcgcgcacg aggccgtcgg tgacgatcgc ggccagcccg
      901 gcgttccgcg ctgcgcccgc gaactggtcg ccgatcgagg cggtgccggc ccaaccgccg
      961 gtggcgatga gaagcacgtc gccgggctgg gcgacgtcga gggcggccag tgcgcccagc
     1021 acgtccgccg gccccgtctc gcaggtcagg gccgggccga cgattctcgt tcccggtgcc
     1081 agctcgagaa ccggccggat tcgatggtcg agcgcccctc gtcccgctct cgcgtcggct
     1141 acctgcgcgg tctcccctgc gccgatccgc ccgatcagct cgggagccgg acgctcgaag
     1201 ccgcggcgga tggtgaggag agggggctgc tcgagcacgg gcctacaggc cggcggggtg
     1261 ccagacggtc ttgagctcca ggaaggcgcc gatctgccac gggctctgct cggcggggtc
     1321 ggcgcggacg acccgcttga cgttgtcggc ggccaggcgc tcgagctcag cgacctcgcc
     1381 gcccgcgccg gtcacgtcga tggagttcac gtccatgtgc gcggcgagga cgggggcgag
     1441 ctcgtcccgg aacccggtga ggacgttcac gacgcccgcc ggcacgtcgg aggtggcgat
     1501 cgcctcggcc agctcgatcg cggcgatcgg gtgtgtttcg gatgcgaccg ccacgagggc
     1561 gttcccgccg acgagagcgg gtgcgagccg ggccacgagg ccgcccagcg ccggctcgtc
     1621 cggcgcgatg atcgccacga cgccggtcgg ctcagggacg gtgaagttga agtacggacc
     1681 tgccaccggg ttcgagctgc cgagcacctg tgcgagcttg tcggcccagc ccgcgtacca
     1741 gatgacgcgg tcgatcgact cttcgacctc gtcccgtccg gaacacgctg cggccaggtc
     1801 ggcggccctt gcctccatca tctcggcgag gcggtagagc acctgccccc ggttgtatgc
     1861 agtcgctcca gcccacttcg ggaaggcgcc ccgcgcggcc cggaccgcat ccctgacgtc
     1921 cttccgtgag gcgcgcgcca cgttctggcc gtcggcctcg aaggtgcgtc cggattccga
     1981 acgcgggaag gcgccgccga gaaagagctt gtacgtctta cggacgcgaa ggcggtcgct
     2041 cattcgaagc ggaggtaggg ctccagcccg tgccggccgc cttcacggcc gaagccggac
     2101 tccttgtagc cgccgaatgg cgacgccggg tcgaagcggt tgaaggtgtt cgcccagacg
     2161 accccggcgc ggagcctctc tgccatccag aggatgcgcg agcccttctc cgtccagaca
     2221 ccggcggaga ggccgtaggc ggtgttgttg gctttctcga gcgcctcttc cggcgtccgg
     2281 aaggtgagca ccgagagcac cgggccgaag atctcctcct gtgcgatccg atagctctgg
     2341 gcgacgttcg tgaacaccgt cggagcgaac cagtatccct tctcgggcag gcggcagggc
     2401 ggctggtaga cctcggcgcc ctcctcctcc ccgctcgcga ccagctcctg gatcttctcc
     2461 agctgctgcc gcgagttgat cgcgcccacg tccgtgttct tgtcgagcgg gtcgccgacg
     2521 cgcagggtgc cgaggcggcg cttgagcttc ttgacaacgg gctcgtagat cgactcctgg
     2581 acgagcaggc gcgaaccggc gcagcacacg tgaccctggt tgaagtagat gccgttgacg
     2641 atcccctcga tcgcctggtc cagtgcgcag tcgtcgaaga cgatgttcgc ggccttgccg
     2701 ccgagctcga gcgtcagggc gatctcgcga cccgcgagct cccgccggat cgccttgccg
     2761 acctcggtcg agccggtgaa ggcgaccttg tcgattccgt cgtggcgcac gaggtgcgcc
     2821 ccggtcgagc cgtcaccggt gacgatgttg accacgcccg gcggcagccc cgcctggcgg
     2881 cagacgtcgg caaagaggag cgccgtcagc ggcgtcgtct cggccggctt gagcacgaca
     2941 gtgttcccgc aggcgagcgc aggcgcgact ttccaggcga gcatcagcaa cgggaagttc
     3001 cacgggatga tctgggcagc gacgccgagc gggcgcggtc gtcgattcgg gaaggcgtac
     3061 tcgagcttgt ccgcccagcc cgcgtagtag aagaagtggg ctgccgcgag cgggagatcg
     3121 acgtcgcgcg actcgcggat cggcttgccc ccgtcgagag actcggcgac cgcgagctca
     3181 cgggcccgct cctgcaggat ccgggcaatc cggaagagat acttcgcccg ctctgcaggc
     3241 cggagctccg accagccgtt ctcgtacgcg ctccgcgcgg ccccgacg
//
LOCUS       DBNGNK010000130         3479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_94116,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000130 DBNGNK010000000
VERSION     DBNGNK010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_94116"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /gene="tmk"
                     /locus_tag="AC150V_03780"
     CDS             complement(<1..303)
                     /gene="tmk"
                     /locus_tag="AC150V_03780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR3762072.1"
                     /translation="MSGRYIAFEGIEGAGKSTVAARVAELLEASGQTVTVVREPGGTP
                     AGEQIRAVLLDPSSDIHPWTEALLFAAARAQLVRDVVTDALARGAWVVSDRSVYSSL"
     gene            complement(300..1682)
                     /locus_tag="AC150V_03785"
     CDS             complement(300..1682)
                     /locus_tag="AC150V_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762073.1"
                     /translation="MTSAAEPRSAFSLVSKGPFSRLWWASVVSSTGDWITIFATIGLG
                     NDIAGGQGVLVAVVSRILPGLVIGGAVGVLTDRVDRRRLIMLADFGRAALVPTLVFAS
                     NLPILVVITIASEFLSILGQSPRAAVVPRLVRDSNIVNANSLILGATYGTIPLGAAFN
                     WLLASLPRVPLDVIPPETEPFALAFLLDSVTFLLSGLLIASLPALRTRTAQALRDQDG
                     DVPSTREDLTEGAKFLWTNRSVRRVIASITASLFGGGVIVAVGPKFVQRTLAAGTTGF
                     FAVVTALGLGAGIGIALVSLYGTKFTRRDVFFAFATLLTGSGLAAAALTGTVFGASAW
                     IFLFGLGAGSGYVMGMTHLHEEVDDSMRGRVFAALFALMRIGIFVSMLVAVPFEATLS
                     RAGVREATRVVLLAGGIVIGLTGLTTVWSLRYLLRRPKLDQETRELLEETTRALRRPR
                     ARDKRKTEPE"
     gene            complement(1797..>3479)
                     /locus_tag="AC150V_03790"
     CDS             complement(1797..>3479)
                     /locus_tag="AC150V_03790"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR3762074.1"
                     /translation="ARRTMSAAQRLYEGGYITYMRTDSITLSEAAIAASRTLVGERYG
                     VEYLPAKPRLYQAKVKNAQEAHEAIRPAGDTFAEPKTVRGAFGPTSAEAKIYDLIWKR
                     TVASQMKNAQGSSVNVRIDGTIASGRVATFAASGKTITFPGFLRAYVEGSDDPDASLD
                     DQEKHLPAMREGDALDVVDLDPRDHETKPPARYSEASLIKRLEELGIGRPSTYASIVS
                     TIQERGYVYKKGTALVPTFTAFATVTLMEQHFGDLVDYAFTARMEDDLDEIATGRQEA
                     IPWLSEFYFGNAHGGLKALVSDHLGDIDAREINSIPIGTDAEGREIVARVGQYGPYLR
                     RGDDTASIPDDLPPDELTLERAVEILEAPNGDRILGTDPEKGLVVIVRSGRFGPYVQL
                     GEPEPDAKEKPKRASLFSSMSIDTVTLEEALKLLGLPRVVGVHPDDGEPITAQNGRYG
                     PYLKWGKETRSLAAEDEIFTIELAAALELLAQPKQRGRRAARPPLRELGDDPNSGKAV
                     VIRDGRYGPYVTDGDYNASLKTSDRVETMTLERAAELLQARRERGPAKRNRG"
BASE COUNT          540 a         1197 c         1197 g          545 t
ORIGIN      
        1 cagcgagctg tagacgctgc ggtccgaaac gacccatgcg ccgcgagcga gcgcgtcggt
       61 gacgacgtcg cgcacgagtt gggcgcgggc ggcggcgaag aggagggcct cggtccacgg
      121 atggatgtcc gacgaggggt cgaggagcac ggcgcggatc tgctccccgg ccggcgtgcc
      181 tcccggctcc cgcaccacgg tgaccgtctg tcccgaggcc tcgagcagct ccgcaacccg
      241 ggccgcaacc gtgctcttgc ccgcaccctc gattccctcg aaggcgatgt agcgcccgct
      301 cactccggct cggtcttccg cttgtctctc gctcggggcc ggcgcagcgc tcgtgtggtc
      361 tcctcgagga gctcgcgcgt ctcctggtcc agcttgggcc gccgcagcag gtaccgcagg
      421 ctccacaccg tggtcagacc ggtgaggccg atcacgatcc cgcccgccag cagcacgacg
      481 cgggtcgctt ctcggacccc cgcccgggac agggtggctt cgaacggcac ggccaccagc
      541 atcgagacga atatcccgat ccgcatcaag gcgaacagcg cggcgaaaac gcggcctcgc
      601 atcgagtcgt cgacctcctc gtggaggtgg gtcatgccca tgacgtatcc cgaaccggcg
      661 ccgaggccga agaggaagat ccatgccgac gcgccgaaga cggtcccggt cagcgcggcc
      721 gcggcgagtc cgctgccggt gagcagcgtc gcaaacgcga agaagacgtc tcgtcgcgtg
      781 aacttggttc cgtacagcga cacgagggcg atcccgatac ctgcaccgag accgagcgcc
      841 gtcaccacgg cgaagaagcc cgtcgtcccg gcggcgagcg tccgctgcac gaacttgggg
      901 ccgaccgcca cgatcacacc gcctccgaac agcgaggcgg tgatgctcgc gatgacccgc
      961 cgcacggacc ggttggtcca gaggaacttc gctccctcgg tgaggtcttc gcgcgtcgac
     1021 ggcacgtcgc cgtcctggtc ccgcagcgcc tgcgcagtcc tcgtccgcag ggcggggagc
     1081 gacgcgatga gcaggccgga gagcaggaac gtgacgctgt cgaggaggaa agcgagggcg
     1141 aacggctcgg tctccggagg gatgacatcg aggggcactc tggggagcga cgcgaggagc
     1201 cagttgaacg ccgcacccag cgggatcgtc ccgtacgtcg ccccgaggat gaggctgttc
     1261 gcgttcacga tgttgctgtc gcgcacgagc cggggaacaa cagcggcacg cggcgactgc
     1321 ccgaggatgc tgaggaactc ggacgcgatc gtgatgacga cgaggatggg gaggttgctc
     1381 gcgaagacga gggtcggcac cagtgcggct cggccgaagt ccgccagcat gatgagccgg
     1441 cgtctgtcca cccggtccgt cagcaccccg accgcgcccc cgatcacgag cccgggaagg
     1501 atgcgcgaga cgacggcgac gaggacaccc tgaccgcctg cgatgtcgtt gccgagtccg
     1561 atcgtcgcga agatcgtgat ccagtcgccg gtgctcgaca cgacgctggc ccaccacagc
     1621 ctcgagaacg ggcccttgga gacgagcgag aacgccgaac gcggctcggc tgcagaggtc
     1681 atggcagcag ggtagatgga tcgagacccg aatcccggtg gccggctcgc caccctccga
     1741 caccggccgc ctcggatctc ggctctgcgg ggcgcgggcc cggccccgaa ccgcgatcag
     1801 ccgcggttgc gcttcgccgg accccgctcg cggcgagcct ggagcagctc ggccgcgcgc
     1861 tcgagcgtca tcgtctcgac tcgatccgac gtcttcaacg acgcgttgta gtcgccgtcg
     1921 gtgacgtacg ggccgtagcg cccgtccctg atcacgacgg ccttgcccga gttgggatcg
     1981 tcgccgagct cccgcagcgg aggtcgggcc gcccggcgcc cccgctgctt cggctgagcg
     2041 agcagctcga gtgcggcagc gagctcgatg gtgaagatct cgtcctcggc cgcgagactt
     2101 ctcgtctcct tgccccactt caggtagggg ccgtagcgcc cgttctgggc ggtgatcggc
     2161 tctccgtcgt cggggtggac gccgacgacc cgcggcaggc cgagcaactt cagcgcctcc
     2221 tcgagcgtca ccgtgtcgat cgacatggac gagaacagcg aggccctctt cggcttctcc
     2281 ttggcgtccg gctccggctc gccgagctgg acgtacggtc cgaaccgccc cgacctgacg
     2341 atgacgacga gccctttctc cggatcggtt ccgaggatcc ggtcgccgtt gggagcctcg
     2401 aggatctcta cggccctctc cagggtcagc tcgtcgggag gcaggtcgtc gggtatcgag
     2461 gcagtgtcgt caccccgcct caggtaaggg ccgtactgac cgacccgggc gacgatctcc
     2521 cgaccctcgg cgtcggtccc gatcggtatc gagttgatct ctcgagcgtc gatgtcgccg
     2581 aggtgatccg atacgagcgc cttcaaccct ccgtgcgcgt tcccgaaata gaactcgctc
     2641 agccacggga tcgcctcctg gcggcccgtc gcgatctcgt cgaggtcgtc ctccatgcgt
     2701 gctgtgaacg cgtagtcgac gaggtcgccg aagtgctgct ccatcagggt gacggtggcg
     2761 aaggcggtga aggtgggaac caacgcagtc cccttcttgt agacgtaccc gcgctcctgg
     2821 atcgtggaga cgatcgacgc atatgtagag ggccggccga tgcccagttc ctcgagacgc
     2881 ttgatgagcg acgcctccga atagcgggcc ggcggcttgg tctcgtggtc cctgggatcg
     2941 aggtcgacga cgtcgagcgc gtccccctcc cgcatggcgg ggaggtgctt ctcctggtca
     3001 tcgagggatg cgtcggggtc gtcgctgccc tcgacgtagg cccggaggaa tccggggaag
     3061 gtgatcgtct tcccggacgc ggcgaacgtc gccaccctcc cggacgcgat cgtcccgtcg
     3121 atccgaacgt tgacgctcga gccctgcgcg ttcttcatct gcgaggcaac ggtgcgcttc
     3181 cagatcaggt cgtagatctt ggcctcggcc gaggtcggcc cgaacgcccc gcggacggtc
     3241 ttgggctcgg cgaaggtgtc accggccggt cggatcgcct cgtgggcctc ctgggcgttc
     3301 ttcaccttcg cctggtacag cctcggcttc gccgggaggt actccacgcc gtaccgctct
     3361 ccgaccagcg tccgggaggc ggcgatggcg gcctcggaca gcgtgatcga gtccgtgcgc
     3421 atgtaggtga tgtagccgcc ctcgtacaag cgctgcgccg cgctcatcgt gcgccgggc
//
LOCUS       DBNGNK010000131         4684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_811511,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000131 DBNGNK010000000
VERSION     DBNGNK010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_811511"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..823
                     /locus_tag="AC150V_03795"
     CDS             50..823
                     /locus_tag="AC150V_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007672144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR3762075.1"
                     /translation="MAKVLVIVPFAFGEGGMANRASQMDDVRIGPEVEFDFRGVKAGP
                     ALFDSYHDWVIADLALFEAGKTAQEEGYDAVCIDTMSDSGMNGRRSVLDIPVIGPGRA
                     SYLTALMLGNTFSVLTQWDPWIGLYKKGLAEYGLADKCVSIRSINKPPDVENLLGGKE
                     EEIFPLLEEAGLKCVEEDGAEVICMGSTTMHQAVPYLREHLPVPVINPGPLTYKLAEF
                     MLGLGLTQSRVAYPSPTVPKHEMLEAMMEGAAASERALA"
     gene            820..2046
                     /locus_tag="AC150V_03800"
     CDS             820..2046
                     /locus_tag="AC150V_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3762076.1"
                     /translation="MTLPLADIRIVAVEQFGAGPWGTLQLADLGAQVIKIEDPASRGD
                     VGRYVPPFQEGEDSLFFETFNRGKQSISLDLRHEGARGVFHDIVRVCDGVYSNLRGDQ
                     PRKLGLTYEQLKDVNKRIVCCSLSGFGMTGPRASEGGYDYMMQGLAGWQSLTGEPGGP
                     PTKSGLSLVDLSGGYASAIALLAGIWRARRDGVGCDCDVSLFETALHELMYIGPWAAT
                     HGYVPPRRGSSAHPTIVPFQNFEAADGWFVVAAAKQKFWEQLCGVIGRPELNDDPRFA
                     TMAGRNEHRDELLPILEDVFRSRTVEEWLEALVGAGIPASRVNSVEEALVDPQTLDRE
                     DVVEHGHPTLGTVRSIRTPLRLADGDEVLEREAERGPFRGEHTTEVLVELCGYTPEQV
                     EELAEAGVFGDTALAR"
     gene            2043..2786
                     /locus_tag="AC150V_03805"
     CDS             2043..2786
                     /locus_tag="AC150V_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007424651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762077.1"
                     /translation="MTARLEDSVAIVTGGARGIGRGIASALAAEGAAVVVADIDGAGA
                     TAVADELVEGGGRALALEVDVSSEADTERMAAATVAGFDRVDVLVNNAGIAGAGEAVH
                     MTLDEWNRVLAVDLTGVFLCCRAVLPTMIAQGSGHIVNIGSQLGLRGAPGLVHYSTAK
                     AGVHGLTKALAREVAEHGVLVNAIAPGPIDTDILADVDDATMAGILAEIPLGRVGSVD
                     EIAPVVVMLAAEGSYFTGSVVNVSGGHVM"
     gene            complement(2804..3634)
                     /locus_tag="AC150V_03810"
     CDS             complement(2804..3634)
                     /locus_tag="AC150V_03810"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3762078.1"
                     /translation="MPYAQIEDGISLYYELTGPEDAPVIIQFGGGLFGRHNFGLVNDG
                     MRENFRLLSFDARGYGASTSPREKYTIEGWADDGEQLLDAVGLDRVFVHGTSMGGMIA
                     IAFTAKHPDRVIAACADVAFAKPDKYRRTLFRVWRRMAESMPWDEFSDHVTTQAVGAG
                     FMESPEGENIFELVREIVGYNDPYTVRQACLAMEDMDLSPLVPDISKPLLMTNGTYDV
                     LCPPDLAPSGLGARKMAELNENITLVEFPDIGHADLVECPDDAVRIVTEFFQRALAPA
                     "
     gene            complement(3735..3926)
                     /locus_tag="AC150V_03815"
     CDS             complement(3735..3926)
                     /locus_tag="AC150V_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3762079.1"
                     /translation="MPIYEYQCSKCEARFEEYLKLSTDPTPPCPECGAKKVERVWSPF
                     ATEWLPSDVAWDRVGRTWD"
     gene            3925..4131
                     /locus_tag="AC150V_03820"
     CDS             3925..4131
                     /locus_tag="AC150V_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762080.1"
                     /translation="MRSQSSQPVPSLDALRELHAALGVHPTEADLAGVLAFLEAILPE
                     LAELEWMLPPEVPPAGLFLPDGDE"
     gene            4128..>4684
                     /locus_tag="AC150V_03825"
     CDS             4128..>4684
                     /locus_tag="AC150V_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3762081.1"
                     /translation="MSLARLAQSLRAGELSPREAVQASLDRIERLDGDLNAFITVRGE
                     EALAEATELERGRERGPLWGAPVAVKDVIDVTGTPTTAGSKILADNVPLRDAATVEGL
                     RRAGAVVVGKLNTHEFAYGARTTSPHFGAAHNPWSLDRICGGSSGGSGAAAAADLVAG
                     TLGTDTGGSVRIPACLCGVTGVRPST"
BASE COUNT          714 a         1593 c         1665 g          712 t
ORIGIN      
        1 ggttaatgtc agacagtcag ctgcgaacga agctcactaa ggagggatca tggccaaggt
       61 gctcgtgatc gtccccttcg cgttcggcga aggaggcatg gcaaaccgcg cgtcgcagat
      121 ggacgacgtc cgcatcggcc cggaggtcga gttcgatttc cgcggcgtga aggcgggccc
      181 cgcgctcttc gacagctacc acgactgggt gatcgccgac ctcgccctct tcgaagcggg
      241 gaagacggcg caggaggagg ggtacgacgc cgtctgcatc gacacgatga gcgactcggg
      301 catgaacggc cgtcgctcgg tgctcgacat cccggtgatc ggcccgggcc gtgcgtccta
      361 cctcacggcg ctcatgctcg gaaacacctt ctcggtgctg acgcagtggg atccgtggat
      421 cggcctctac aagaagggtc tggccgagta cgggctggcc gacaaatgcg tctcgatccg
      481 ctcgatcaac aagcctccgg acgtcgagaa cctcctgggc ggcaaggagg aggagatttt
      541 cccgctgctc gaggaggccg gcctcaagtg cgtggaggag gacggagccg aggtcatctg
      601 catgggctcg acgacgatgc accaggccgt gccgtacctg cgcgagcacc tgccggttcc
      661 cgtgatcaac ccggggcccc tcacgtacaa gctcgccgag ttcatgctcg gcctggggct
      721 gacgcagagc agggtcgcgt atccgagccc gaccgtgccg aagcacgaga tgctcgaggc
      781 gatgatggag ggagctgccg catcggagcg tgccctcgcg tgacgctgcc gctcgcggac
      841 atccggatcg tcgcggtcga gcagttcgga gcgggcccgt ggggcacgct gcagctggcc
      901 gacctcggcg cgcaggtgat caagatcgag gatccagcgt cgcgggggga tgtcggccgc
      961 tacgtccctc ccttccagga gggcgaggac tccctcttct tcgagacctt caaccgcggc
     1021 aagcagagca tctcgctcga cctccgccac gagggggcgc gcggcgtctt ccacgacatc
     1081 gtccgcgtct gcgacggcgt ctactccaac ctgcgcggcg accagccccg caagctcggg
     1141 ctgacgtatg agcagctcaa ggacgtgaac aagcgcatcg tctgctgctc actctcgggg
     1201 ttcgggatga ccgggccgag ggcgtcggag ggcggctacg actacatgat gcaggggctc
     1261 gcgggctggc agagcctcac gggtgagccg ggcgggcccc cgaccaagag cggcctctcc
     1321 ctcgtcgacc tctccggcgg gtacgcgtcg gcgatcgcgc tgctggccgg gatctggcgc
     1381 gcccgtcgcg acggcgtcgg ctgcgactgc gacgtctcgc tcttcgagac cgcgctccac
     1441 gagctcatgt acatcggccc ctgggctgcg acacacggct acgtgccgcc gcgccgtggc
     1501 agctcggcgc acccgacgat cgttccgttc cagaacttcg aggcagcgga cggctggttc
     1561 gtcgtcgccg ccgcgaaaca gaagttctgg gagcagctct gtggcgtgat cggccgaccc
     1621 gagctgaacg acgaccctcg cttcgccacc atggcgggcc ggaacgagca ccgcgacgag
     1681 ctgctcccga tcctcgagga tgtcttccgc agccgtacgg tggaggagtg gctcgaggcg
     1741 ctcgtcggag ccggcattcc ggcgtcgagg gtgaactccg tcgaggaggc gctcgtggat
     1801 ccgcagaccc tcgaccgcga ggacgtcgtc gagcacggcc acccgacgct cggaaccgtc
     1861 cgctcgatcc gcacgcccct gcggctcgcc gacggcgacg aggtgctcga gcgggaagcc
     1921 gagcgcggtc cctttcgcgg ggagcacacg accgaggtgc tcgtcgagct ctgtggctac
     1981 acaccggagc aagtggagga gctggccgag gccggcgtgt tcggcgatac ggcgctggcg
     2041 cggtgaccgc ccgcctggaa gacagcgtcg cgatcgtaac gggtggcgcc cgtggcatcg
     2101 ggcgcgggat cgcgtccgcg cttgccgccg agggagcagc ggtcgtcgtt gccgacatcg
     2161 acggcgccgg cgcgacggcg gtcgcggacg agctcgtcga gggcggcgga cgcgcgctcg
     2221 cgctcgaggt cgacgtgagc tccgaggccg acaccgagcg gatggcggct gcgacggtgg
     2281 cagggttcga tcgcgttgac gtcctcgtga acaacgcagg catcgccggg gcgggcgagg
     2341 ccgtgcacat gacgctcgac gagtggaacc gcgtgctcgc ggtcgacctg accggcgtgt
     2401 tcctctgctg tcgagcggtt ctgccgacga tgatcgcgca gggaagcggg cacatcgtga
     2461 acatcggctc gcagctcggg ctgcggggcg ctccaggcct cgtgcactac tccaccgcga
     2521 aagcgggggt gcacgggctg acgaaggcgc tggcgcgcga ggtcgccgaa cacggagtcc
     2581 tcgtcaacgc aatcgccccc ggtccgatcg acacggacat cctcgcggac gtcgacgacg
     2641 cgacgatggc gggcatcctc gccgagatcc cgctcggccg cgtcggctcc gtagacgaga
     2701 tcgcgccggt cgtcgtcatg ctcgccgccg agggctcgta cttcacgggg tcggtggtga
     2761 acgtgtccgg cggccacgtg atgtgaccgt gttcgccgtg gcctcaggcg ggggcgagcg
     2821 ccctctggaa gaactcggtg acgatccgga cggcgtcgtc gggacattcg acgaggtccg
     2881 cgtggccgat gtccgggaac tcgacgagcg tgatgttctc gttcagctcg gccatcttcc
     2941 gcgcgccgag gccggacggc gcaaggtcgg gcgggcagag cacgtcgtac gtcccgttcg
     3001 tcatcagcag cggcttcgag atgtcgggca cgagcggcga gaggtccatg tcctccatgg
     3061 ccaggcacgc ctgccgcacg gtgtaggggt cgttgtagcc gacgatctcc cgcacgagct
     3121 cgaagatgtt ctcgccctcc gggctctcca tgaagcccgc gccgaccgcc tgggtcgtca
     3181 catggtcgga gaactcgtcc cacggcatcg actcggccat ccgccgccag acgcggaaga
     3241 gcgtgcgccg gtacttgtcc ggcttcgcga aggcgacgtc ggcacaggcg gcgatgacgc
     3301 ggtcggggtg cttcgccgtg aaggcgatcg cgatcatccc gcccatcgac gtgccgtgca
     3361 cgaacacccg gtcgagaccg acggcgtcca gcagctgctc gccgtcgtcg gcccagccct
     3421 cgatcgtgta cttctcccgc ggactggtcg acgcgccgta gccgcgagcg tcgaacgaga
     3481 gcagccggaa gttctcccgc atcccgtcgt tgacgagacc gaagttgtgc cggccgaaga
     3541 ggccgccgcc gaactggatg atcacgggcg cgtcctccgg cccggtcagc tcgtagtaga
     3601 ggctgatccc gtcctcgatc tgtgcatacg gcaccggtcg cccacctcct cgcatgcttg
     3661 acagcagcgc aaagtctgac ctatacgaat ccgctatgcc accgactgcc gcgcgggggc
     3721 gggcgtgtcg ccggtcagtc ccaggtgcgg ccgacgcggt cccacgccac gtcgctcggc
     3781 agccactcgg tggcgaaggg cgaccagacc cgctcgacct tcttggcgcc gcattctggg
     3841 cagggtggcg tcgggtcggt ggagagcttg aggtactcct cgaaccgcgc ctcgcacttc
     3901 gagcactgat attcgtagat cggcatgcgc agtcagtcta gccagccggt tccgtcgctc
     3961 gatgctctcc gcgagctgca cgcggcgctg ggcgtgcacc ccacggaggc cgatctcgcc
     4021 ggcgtgctcg ccttcctcga ggcgatcctg cccgagctcg cggagctgga atggatgctc
     4081 ccgccggagg tcccgccggc aggcctcttc cttccggacg gcgacgaatg agcctggcgc
     4141 ggctcgcgca gagccttcga gcgggggagc tctccccgcg tgaggccgtc caggcgtcgc
     4201 tcgaccggat cgaacgcctc gacggcgacc tcaacgcgtt catcaccgtc cgcggcgagg
     4261 aggcgctggc ggaggcgacg gaactggagc gcgggcgcga acgcggcccg ctctgggggg
     4321 cgccggtggc ggtgaaggac gtgatcgacg tgacgggcac ccccacgacg gccgggtcga
     4381 agatcctcgc cgacaacgtg ccgctgcgcg atgccgcgac ggtcgagggc ctgcggcgcg
     4441 cgggggctgt cgtcgtcggg aagctgaaca cccatgagtt cgcctacggc gcccgcacca
     4501 cgagcccaca cttcggtgcc gcgcacaacc cctggagcct cgaccggatc tgcggcggct
     4561 cgagcggcgg gagcggcgcg gccgccgcgg cggacctcgt cgccggcacg ctcggcaccg
     4621 acacgggcgg ctccgttcgg attccagcct gtctgtgcgg cgtcaccggc gtccgcccgt
     4681 cgac
//
LOCUS       DBNGNK010000132         8214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_94493,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000132 DBNGNK010000000
VERSION     DBNGNK010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8214
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_94493"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="AC150V_03830"
     CDS             complement(<1..1023)
                     /locus_tag="AC150V_03830"
                     /inference="COORDINATES: protein motif:HMM:NF046871.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HIR3762082.1"
                     /translation="MSRRAATIVIVLLAAALAGVGALALPSSPLHDEVTLGLDLQGGL
                     EVTLQAVPPKDRDLTDEDLDRSVSIMRNRVDKLGVAEPEIRKQGDDQIVIQLPGVSDP
                     EAAAGVIGKTAQLELYDLEANLVAPSISAQRIPIATDSLHGLLAGQQSLVEEDNAESW
                     YLFNKRKQLVAGPAASKEEVLKGRGGKLPDGWFVFATPPNTVVLVCGVDEVVCPGVQE
                     ANPQKDYFYLIRYDPPSIPEMTGADLELSGTRSDFDPQTGQPIVTLAFTGAGADKFQE
                     ITREEAQRGKLLANTIGQGQDPQAFLQHFAIVLDREIKSWPSIDFEQYPGGISGNNGA
                     QITGLGD"
     gene            complement(1063..1434)
                     /gene="yajC"
                     /locus_tag="AC150V_03835"
     CDS             complement(1063..1434)
                     /gene="yajC"
                     /locus_tag="AC150V_03835"
                     /inference="COORDINATES: protein motif:HMM:NF014725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR3762083.1"
                     /translation="MSSLLIILAMFVLLWVLMIRPQRSRQRQQKAMLDTIDTGDEILT
                     HGGLYGIVQDFDENEDLIVEIAEGINVRLARRSVASLVKPDDEEDEEIEAGEASEEDA
                     AVDAEEGIVKNEEETVKAGES"
     gene            complement(1431..2609)
                     /gene="tgt"
                     /locus_tag="AC150V_03840"
     CDS             complement(1431..2609)
                     /gene="tgt"
                     /locus_tag="AC150V_03840"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015946383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR3762084.1"
                     /translation="MGWGAPQAIGRKGQAVGVSAFTVQAIDGAARAGTIQTAHGDIST
                     PAFMPVGTKGTVKSLHPDEVRALGAEVILGNTYHLHFRPGEDVIGQLGGLHAFTGWDG
                     PILTDSGGFQVFSLRDTLVAVDDDGVTFRSVYDGSAERFTPEGVAAIQRRLGSDVAMC
                     LDICPPAAASPREHATAVRRTSLWARRQRRCERAPGQLLFGIAQGGTDEELRRQSIAE
                     ITELDFDGHALGGLAVGEDRGEMFDTVAWAAPLLPAEQPRYFMGIGDAEGILEVVERG
                     IDMFDCVLPTRTARTGSALTGRGRLNLKNARFARDRRPLEEGCGCPACERFSRAYVRH
                     LVNQEELLGLRLLSLHNLWFVLRLTAGAREAITRGELAAYKADMLAGLAGGVEEEERP
                     "
     gene            complement(2697..3638)
                     /gene="queA"
                     /locus_tag="AC150V_03845"
     CDS             complement(2697..3638)
                     /gene="queA"
                     /locus_tag="AC150V_03845"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR3762085.1"
                     /translation="MRSSELEYDLPPELIAQQPLPQRDASRLLVYRRSTGEVSHRVVS
                     ELPELLSGCLAVINDTRVIPARLRLRRASGGEVEILLLERLSANGTWEALARPSRRLR
                     PGEHLGPVELLEPLGDGRWRIRLSGEPEGETPLPPYIHETLADASRYQTVYAAEPGSA
                     AAPTAGLHFTPELLSQLDVERVTLHVGLDTFRPVTAPRLAEHALHGERYQVLPRAWAR
                     IDGAQTVVAVGTTSVRVLETVARTGGLAGRTDLFITPGFEFRRVDALLTNFHLPRSSL
                     LALVMAFSGVEETRNLYRVAIEKEYRFYSFGDAMLVL"
     gene            3665..4144
                     /locus_tag="AC150V_03850"
     CDS             3665..4144
                     /locus_tag="AC150V_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762086.1"
                     /translation="MDDTALERVRERLADAQAERVDGARLEAALERSRSQIDALSSAA
                     AELEHTLPDRISDAVQEGFRREVLPVGRNLAEIRGLLNQVLRRLERLEQELLAERHAR
                     IDDLGLLVELVSTSWRGVDERLRRLEEAGTSVIPLRTDPYDEPSESNGAHAAAVAGR"
     gene            complement(4141..5556)
                     /locus_tag="AC150V_03855"
     CDS             complement(4141..5556)
                     /locus_tag="AC150V_03855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3762087.1"
                     /translation="MFGSLRVRLPALFLLGVAVTGIVAALIALRLFQDYTSAQSYAEL
                     QREARGLALLYSQSALRAADEGETAPDFAAATLELATGDRLYYVGANVFPGQGSGLNR
                     LPEDALEPEVVSGGEAMTFEFTPPGENRVFLAASQPVRLEPGAPPFGTLVVAKPRTEV
                     RDQWVPLVQRLAFAFLAGVVIAGALSWWLSRRITKPVEALSDAADEIAAGNYAVSVPV
                     GLGGDEISHLSQRFNEMAQKLSESEQRERQFLMSVSHELRTPLTAIRGHVDAIRDGLV
                     EDPELIRHSLDVVAAESQRLERLVGDVLDLAKLNAHRFTVLTEEVDMGRLVEQAYTAF
                     GEEARRREIEYRLSEDGEPPTAFTDGDRVLQVITNLLANAFRWTPDGGRIDLGLGADN
                     GTINVEIADTGPGIDDSEQERIFRPFVSDDMDGTGLGLPIARELALALGGRIELESTV
                     GEGSCFRLALPARRGLEPLRD"
     gene            complement(5578..6285)
                     /locus_tag="AC150V_03860"
     CDS             complement(5578..6285)
                     /locus_tag="AC150V_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3762088.1"
                     /translation="MTATPTILVVEDESSIASFISLYLKNAGYNVRAVSTGSAALNHV
                     ATEAPSLILLDLNLPDMDGIEICRRVRKTSDVPILMLTARDEDVDKIIGLEVGADDYL
                     TKPFNPRELVARVKSVLRRSAPERRRDDSQEIRHGEVLINAGRREVLVGEDEIQLAPK
                     EFDLLWELLDHRGLVLTRDQLLERVWGYTFAGDTRTVDVHVRQIRRKLGDASPIVTVW
                     GVGYKVASERSSPAPAA"
     gene            complement(6282..6851)
                     /locus_tag="AC150V_03865"
     CDS             complement(6282..6851)
                     /locus_tag="AC150V_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762089.1"
                     /translation="MRILLATLFVVALAVPTAALASERPPVGSLSIEEGSGVVMLKGV
                     GGVLGKLDGVLQIVDLTPNDRWRPIVNGKVVKRPRVRLRGDAISFRVLGGKFKIVMRG
                     ENVSVSARGRGTAVLRGDPDAFGYAGLYSTDADVDCIGLPEACEVVPGFATRIEYGMP
                     AARGGDPAREKERDTGSAKSDKPERGASS"
     gene            complement(6944..7873)
                     /locus_tag="AC150V_03870"
     CDS             complement(6944..7873)
                     /locus_tag="AC150V_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015110125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR3762090.1"
                     /translation="MTFDFLILFAGLLCAATGGELFVRGAVGIAAASRIPPGIIGATV
                     AAFGTSSPEISVAINSALDGRPQLALGDALGSNVANIGLVLGLMLMVGSVKVAGGTIR
                     RDIAAALIVPLVTAGLALDGRLSRADAALLFSVFCVWLIATVLAARRERSAAAEVLGE
                     HSFRRAIPVGLAGLVLLVVAGRLIVSGAKGIGAELGLSAFVVGVVFVAVGTSVPELAT
                     ALISRLRGHEEIGLGTVLGSNIFNGALIIPIAAIINPIRVDWGEISISLAFGIVLVLA
                     VLPIPTPILGRRRGALLLSLYLSSILVLLYTHG"
     gene            8000..>8214
                     /locus_tag="AC150V_03875"
     CDS             8000..>8214
                     /locus_tag="AC150V_03875"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR3762091.1"
                     /translation="MDVLALVIFVAALTVIAFEWAHRTKVALAGAGLMVLIGIIDQEN
                     AIESVNWATLGLLTGMMVLVALAEPTG"
BASE COUNT         1334 a         2851 c         2778 g         1251 t
ORIGIN      
        1 gtcaccgagt cccgtgatct gggcgccgtt gttacctgag atgccgccgg ggtactgctc
       61 gaagtcgatc gacggccacg acttgatctc gcggtcgagc actatcgcga agtgctgtag
      121 gaacgcctgt gggtcctggc cctgtccgat cgtgttggcg aggagcttgc cccgctgcgc
      181 ctcctcgcgc gtgatctcct ggaacttgtc ggcgccggcg ccggtgaagg cgagcgtcac
      241 gatcggctgg cccgtctgcg ggtcgaagtc ggagcgggtg ccggacagct cgaggtcggc
      301 gcccgtcatc tccgggatcg acggcgggtc gtagcggatg aggtagaagt agtctttctg
      361 tgggttggcc tcctggacac ccgggcagac gacctcgtcg acaccgcaga cgaggacgac
      421 ggtgttgggc ggggtcgcaa acacgaacca gccgtcggga agcttgccgc cgcgcccctt
      481 gagcacctct tccttcgacg cggccggccc ggcgacgagc tgcttacgct tgttgaagag
      541 gtaccacgac tcggcgttgt cctcctccac gagcgactgc tgcccggcga ggaggccgtg
      601 gagcgagtca gttgcgatcg ggatgcgctg cgcgctgatc gagggcgcga cgaggttcgc
      661 ctcgaggtcg tagagctcga gctgcgcggt cttcccgatc acgcccgcgg ccgcctcggg
      721 atcgctcacc ccgggcagct ggatgacgat ctggtcgtct ccttgcttgc ggatctcggg
      781 ctcggcgacg ccgagcttgt cgacccggtt tcgcatgatc gagaccgagc ggtcgagatc
      841 ttcatcggtg aggtcgcggt ccttgggcgg gacggcctgc agcgtgacct ccaggccccc
      901 ctgcaaatcg aggccgagcg tcacctcgtc gtgcagcggc gacgagggaa gcgcgagcgc
      961 ccccactccc gcgagcgccg cggcgaggag gacgatgacg attgtggctg cgcggcgact
     1021 cacgtggctc gatcagggta gcggggcatc ttctgccggt ttctacgact cgccggcttt
     1081 tacggtttcc tcctcgttct taacgatccc ttcttcggcg tcaaccgcag cgtcctcttc
     1141 cgacgcctct ccggcttcga tctcctcgtc ttcctcgtcg tcgggcttga cgagcgaagc
     1201 gacggagcgc cgtgccaggc ggacgttgat tccttcggcg atctcgacga tcagatcctc
     1261 gttctcgtcg aaatcctgga cgatgccgta aaggcccccg tgcgtcagga tttcgtctcc
     1321 tgtatcgatt gtgtccaaca tcgccttctg ttgtcgctgg cgagagcgct gaggacggat
     1381 catcagcacc cacagcagca cgaacatcgc gagaatgatc agcagcgagc tcaaggtcgt
     1441 tcttcctcct ctacgcctcc ggcgagccct gcgagcatgt cagccttgta ggcagcgagc
     1501 tcgccccgcg tgatcgcttc gcgcgcgccg gcggtgagtc gtaatacgaa ccacagatta
     1561 tgcaggctga gcagccgcag cccgagcagc tcctcctggt tgacgaggtg gcggacgtac
     1621 gcgcgcgaga agcgctcgca ggccgggcag ccacagcctt cctccagcgg ccgccggtca
     1681 cgggcgaagc gggcgttctt cagattcagc cgcccgcggc cggtgagcgc tgacccggtg
     1741 cgcgccgtgc gggtcggcag aacgcagtcg aacatgtcga ttccgcgctc cacgacctcg
     1801 aggatcccct cggcgtcgcc gattcccatg aagtaacgag gctgctcggc cggcagcagc
     1861 ggtgcggccc acgcgaccgt gtcgaacatc tcgccgcgat cctctccgac ggccagcccg
     1921 ccgagggcgt gcccgtcgaa gtcgagctcg gtgatctccg cgatggactg ccggcgaagc
     1981 tcttcgtcgg ttccaccctg cgctatcccg aagagcagct ggccgggcgc ccgctcgcac
     2041 cggcgctggc gccgggccca gagcgacgtg cgcctcaccg ccgtggcgtg ttctcgggga
     2101 gatgcggcgg cgggcgggca gatgtccagg cacatggcga cgtccgagcc cagccgccgc
     2161 tggatcgcgg cgacaccttc gggcgtgaag cgctccgccg agccgtcgta gacggaacgg
     2221 aacgtgacgc cgtcgtcgtc gaccgcgacc agtgtgtcgc ggagagagaa cacctggaag
     2281 cctcccgaat cggtgaggat cgggccgtcc cagccagtga acgcatgcag gccgccgagc
     2341 tggccgatca cgtcctcgcc cggacggaag tgcaggtggt aggtgttccc gaggatcacc
     2401 tccgcgccca gcgccctgac ctcgtccggg tggaggcttt tcacggttcc cttcgtgccg
     2461 acgggcatga aggcgggtgt cgagatgtcg ccgtgggcgg tctggatcgt ccccgcgcgt
     2521 gccgcgccgt cgatcgcctg aacggtgaac gcactcacgc cgacggcctg tcccttccgg
     2581 ccgatggcct gaggggcacc ccagcccacc acccgctgcc gatcctagtc gtggactgcg
     2641 cgcgcatcgg acaccggatc gtgccggaaa accggcagtt cctccaccgg caccgatcag
     2701 aggacgagca tcgcgtcacc gaaggagtag aaccgatact ccttctcgat cgcgacgcgg
     2761 tagaggttcc tcgtctcctc caccccggag aacgccatca cgagcgccag caacgacgaa
     2821 cgcggcaggt ggaagttggt gaggagcgcg tcgacccgcc ggaactcgaa cccaggagtg
     2881 atgaagaggt cggtgcggcc ggcgagcccc ccggtccggg cgaccgtttc gaggacgcgg
     2941 acggaggtcg ttccgacggc gacgaccgtc tgggcgccgt cgatacgcgc ccaggcccgc
     3001 ggcaggacct ggtagcgctc cccatgcaga gcgtgctcag cgagccgcgg cgccgtgacc
     3061 ggcctgaagg tgtcgagccc cacgtgcagg gtcacccgct ccacgtcgag ctgcgagagc
     3121 aactcgggcg tgaaatggag accggcggtc ggcgctgccg cggaaccagg ctcggccgcg
     3181 tagaccgtct ggtagcggct cgcgtcggca agtgtttcgt ggatgtacgg gggaaggggc
     3241 gtctccccct ccggctcccc gctgaggcga atgcgccagc gcccatctcc gagcggctcg
     3301 agcagctcca caggaccgag gtgctcgccc gggcggaggc gccgggaggg ccgtgccagg
     3361 gcctcccagg ttccgttcgc gctcagacgc tcgagcagca ggatctccac ttcgccgccg
     3421 ctcgcacgcc ggagccgcag ccgagccgga atcacgcgag tgtcgttgat cacagccagg
     3481 cagccggaga gcagctccgg cagctcgctc accacgcggt ggctcacctc acccgtcgag
     3541 cgccggtaga cgagcagccg tgaggcgtcg cgctgcggca acggctgctg cgcgatcagc
     3601 tccggaggca ggtcgtactc gagctccgac gatctcatcc gacggccaga gtagccttgc
     3661 cgccatggac gacaccgcgc tcgaacgggt tcgagagcgc cttgcggacg cccaggccga
     3721 gcgtgtggac ggcgcgcgcc tggaggcggc gctggagcgc tcgcgcagtc agatcgacgc
     3781 gctctcctcg gccgccgccg agctcgagca cacgctgccc gaccggatca gtgacgcggt
     3841 ccaggagggc ttccggcgcg aggtgctgcc cgtgggccgc aatctcgccg agatccgtgg
     3901 actgctgaac caggtgctac gacggctcga gcggctcgag caggaactgc tcgccgagcg
     3961 gcacgcccgc atcgacgacc tcggcctcct cgtcgagctc gtgtcgacga gctggcgggg
     4021 cgtcgatgaa cgactgcgtc ggctcgaaga ggctggaacg agcgtcatcc cgctgcgcac
     4081 cgacccgtac gacgagccgt ccgagagcaa cggcgcccac gcggcggccg tcgccggtcg
     4141 ctagtcccgc agcggctcga gaccgcgacg cgcgggcagc gcgagtcgga aacagctccc
     4201 ctcgccgacc gtcgactcca gctcgatccg accaccgaga gccagtgcca gctctctcgc
     4261 gatgggcagg ccgagacccg tgccgtccat gtcgtcggag acgaacgggc ggaagatgcg
     4321 ctcctgctcg gaatcgtcga tacccggtcc ggtgtcggcg atttcgacgt tgatcgtgcc
     4381 gttgtccgcc ccgagcccga gatcgatccg gcccccgtcg ggcgtccagc gaaaggcatt
     4441 ggcgagcagg ttcgtgatca cctggagcac gcggtcgccg tccgtgaacg cagtcggcgg
     4501 ctccccgtcc tcgctcaggc ggtactcgat ctcccgccgc cgcgcctcct ccccgaacgc
     4561 ggtgtacgcc tgctcgacca gtcgccccat gtcgacctcc tctgtcaaca cggtgaacct
     4621 gtgtgcgttc agtttcgcca gatcgagcac gtcgccgacg agacgctcga gccgctgcga
     4681 ctcggccgcg accacatcga gcgagtgacg tatcagctcg ggatcctcga cgaggccgtc
     4741 gcggatggcg tccacgtggc cgcgaatcgc ggtcagcggc gtgcgcagct cgtgggagac
     4801 cgacatcaag aactggcgct cgcgctgctc cgactccgac agcttctgcg ccatctcgtt
     4861 gaaccgctgc gagaggtggc tgatctcgtc accgcccaga ccgacgggca ccgataccgc
     4921 atagttgccg gccgcgatct cgtcggcggc gtcagagagg gcttcgacgg gtttcgtgat
     4981 tcgccgagac agccaccagg acagcgcgcc cgcgatgacg actcccgcga ggaaggcgaa
     5041 tgccagccgc tgcacgagcg gcacccactg gtcgcgcacc tcggtccgtg gtttcgcgac
     5101 gacgagcgtg ccgaaggggg gcgctccagg ctcgaggcgc accggctggg aggcggcgag
     5161 gaacacacgg ttctcgcccg gcggcgtgaa ctcgaacgtc atcgcctccc cgccgctcac
     5221 cacctccggc tcgagcgcgt cttcgggcaa ccggttcagc cccgatccct ggccgggaaa
     5281 cacgttcgca ccgacgtagt agagacggtc gcccgttgca agctcgagcg tcgctgcagc
     5341 gaagtcaggg gccgtctcgc cttcgtccgc ggcgcgcagc gccgactgcg agtagagcaa
     5401 cgcgagaccg cgcgcctcac gctgcagctc cgcgtagctc tgcgcgctcg tgtagtcctg
     5461 gaagaggcgg agcgcgatca gcgcagcgac gatgccggtg accgccactc cgagaaggaa
     5521 gagggccgga agcctgaccc gcagtgaccc gaacacgccg agtccttcgc cgaggcgcta
     5581 cgcggccggc gcggggctcg accgctcgga ggcgaccttg tagcccacgc cccagaccgt
     5641 gacgatgggc gacgcatccc cgagcttgcg ccggatctga cgcacgtgca cgtcgacggt
     5701 gcgcgtgtcc ccggcaaagg tgtagcccca gacgcgttcg aggagctggt cgcgcgtcaa
     5761 taccagcccg cggtgatcga gcagctccca cagcagatcg aattccttcg gcgcgagctg
     5821 gatctcgtcc tcgccgacca gcacttcacg tcggcccgcg ttgatcagaa cctccccgtg
     5881 ccggatctcc tgcgaatcgt cgcgacggcg ctccggcgcc gaacggcgga ggacgctctt
     5941 cacgcgcgcg accagctcac gggggttgaa cggcttcgtc aggtagtcgt cggcgccgac
     6001 ctcgagaccg atgatcttgt cgacgtcttc gtcgcgcgcg gtaagcatca gaatcgggac
     6061 gtcagacgtc ttgcggacac ggcggcagat ctcgatgccg tccatgtccg ggagattcag
     6121 gtcgagcagg atcagcgacg gcgcctcggt ggcgacgtga ttcagggcag cgctgcccgt
     6181 cgacacggcg cgcacgttgt agcccgcgtt cttcaggtac agggagatga acgaggcgat
     6241 cgacgactcg tcctcgacga cgaggatcgt gggggtagcg gtcacgacga tgctcctctc
     6301 tcgggcttgt cggacttcgc gctccccgtg tccctctcct tctcccgcgc tggatcgccc
     6361 ccgcgtgcgg ccggcattcc gtattcgatg cgcgttgcga aaccgggcac cacttcacac
     6421 gcctcgggca ggccgatgca gtccacatcc gcatccgtgg agtagagacc ggcgtacccg
     6481 aacgcgtcgg gatcgcctct caggacggcc gtgccacggc cgcgagccga gaccgagacg
     6541 ttctcgcccc gcatgacgat cttgaacttc ccgccgagga cacggaagct gatcgcgtcg
     6601 ccgcgaaggc gcacgcgggg gcgcttcacg accttgccgt tcacgatcgg acgccagcgg
     6661 tcgttcggcg tcagatcgac gatctgcagc acaccgtcga gctttccgag cacgccgccg
     6721 acacccttca gcatcaccac gccgctcccc tcctcgatcg agagggaccc cacgggcggg
     6781 cgctcggagg cgagcgcggc ggttggcacg gcgagcgcga cgacgaacag agtggcgagc
     6841 aggattcgca tcagccttcc gagtatcggc aaacgaggtg ggttcccggt gagatacggg
     6901 taaggaaagt gtaagccccg cccggccgag aagccgcggg ccgctacccg tgcgtgtaga
     6961 ggagcaccag gatcgacgag agatagagcg acaacagaag cgcgcctcgc cgacggccca
     7021 ggatcggcgt ggggatcgga agcaccgcaa gcaccagcac gatcccgaac gccaggctga
     7081 tcgagatctc cccccagtcc acgcggatcg ggttgatgat cgcggcgatc gggatgatca
     7141 gggcgccgtt gaagatgtta ctgccgagca ccgtgccgag cccgatctcc tcgtgaccgc
     7201 gcagacgcga gatcagcgcc gtcgcgagct ctggaacgga agtgccgacc gcgacgaaga
     7261 cgacgcccac cacgaaggcg ctcaggccga gctcagctcc gatgcccttc gcccccgaca
     7321 cgatcaggcg gccggccacg acgagcagga cgagtcccgc cagcccgact ggaatcgccc
     7381 ggcggaagct gtgctctccc agaacctcgg ccgccgcgct ccgctcgcgt cgcgccgcaa
     7441 gcaccgtcgc gatcagccag acgcagaaga ccgagaagag aagcgccgcg tcggccctgc
     7501 tcagccgacc gtcgagggcc agcccggccg tcacgagcgg gacgatcagc gccgcggcga
     7561 tgtcgcgcct gatcgtgccc ccagcgacct tcacggagcc gaccatcagc atcaggccga
     7621 gcacgagccc gatgttggcc acgttgctcc cgagggcgtc gccgagcgcg agctgcggcc
     7681 gtccgtcgag ggccgagttg atcgcgaccg atatttccgg gctcgacgtt ccgaaggccg
     7741 cgaccgtcgc gccgatgatc ccgggcggta tccgtgacgc cgcggcgatg ccgaccgcgc
     7801 cgcgcacgaa cagctcgccg ccggtggcgg cacagaggag gccggcgaag aggatgagga
     7861 agtcgaacgt catcgggtcg gtgaaccggc gccgaccagc cttcccggca cgccgcccgg
     7921 gactctagtt gatcggctcg agaacacgcg cgccgccacg cggcgggagc cctgccacag
     7981 gacgggtagg ctcgccaacg tggacgtcct tgccctcgtc atcttcgtcg cggcgctgac
     8041 cgtgatcgcg ttcgaatggg cgcatcgcac gaaggtcgcc ctcgccggcg cgggcctgat
     8101 ggtgctgatc gggatcatcg atcaggagaa cgcgatcgaa tccgtcaact gggccacgct
     8161 cggcctcctg acggggatga tggtgctcgt cgccctcgcc gagccgaccg gcat
//
LOCUS       DBNGNK010000133         4251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1956734,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000133 DBNGNK010000000
VERSION     DBNGNK010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1956734"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="AC150V_03880"
     CDS             complement(<1..488)
                     /locus_tag="AC150V_03880"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3762092.1"
                     /translation="MRDRFERIYTAVLTDVLDALGYRHQTLPPEIRPLVPGMRLAGQA
                     FTVQGRPTDTQDWDAAIRKTLAMLGSVPSGHVAVYACHQDIAAHFGELSATSLLSRGV
                     AGCVLDGGCRDVRFIVDEGFPVFTRFTTPEDSTWRWQVESTQAPITIGRVRIEPGDWV
                     VG"
     gene            complement(485..1348)
                     /locus_tag="AC150V_03885"
     CDS             complement(485..1348)
                     /locus_tag="AC150V_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762093.1"
                     /translation="MSELRFADGLRYRVEIPSVEGPAVFRAVLDEAQARDVPVRRVSQ
                     GSGVMMLTDAEIAEMAALGAEHGIEVSLFLGPRGAWDAGGQSLATSAVGGVARGSDGV
                     EWCVAEVQRGLSLGIRSFLVADIGVLARLGELKRRGELPESLVLKTSVLLPCANPVTA
                     ATLESLGAGTINVSTDLSPAELGELRAACDAPLDVYVEAPDDQGGFVRFYEVAEMIRL
                     AAPMYVKLGVRNAPNIYPVGLHMADTAAKLGRERVRRTELVLRLLRERAPDLVESRGD
                     DTPGDLAIPMP"
     gene            complement(1370..1762)
                     /locus_tag="AC150V_03890"
     CDS             complement(1370..1762)
                     /locus_tag="AC150V_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3762094.1"
                     /translation="MGTAENERVVRGLFEAFARRDGFALREVFAPDAEWVVPGEGVMA
                     GTYTGREEIIRFLARLPKETGGTYGSRLVDVLVSEKRAAALYRAFGERRGRSLDIDQV
                     LLVGFEGGLVRTVLALPANPAAFDEFWA"
     gene            complement(1749..1961)
                     /locus_tag="AC150V_03895"
     CDS             complement(1749..1961)
                     /locus_tag="AC150V_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762095.1"
                     /translation="MTDQATVSLLRWLRRQLRQPTPMREHLEAAVENDDPDEVRRLLR
                     HFDFTDEQRRHVDGLIDRWEESSGDG"
     gene            1981..2496
                     /locus_tag="AC150V_03900"
     CDS             1981..2496
                     /locus_tag="AC150V_03900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3762096.1"
                     /translation="MDTFLAIASRRDERSYTSDPLPDGVVARILDAGRLSGSSKNRQA
                     WVFVVLDDRRRIEQLAAAVYVPENVLSAGLVVAILVSGKGPTSFDAGRAAQNMLLAAW
                     NEGVSSCPNGIADSDAAAAALEAGDDERPVIVLTFGAPRRPRDAESRDASEWSARANR
                     KPVDEVVRRLA"
     gene            2493..3434
                     /locus_tag="AC150V_03905"
     CDS             2493..3434
                     /locus_tag="AC150V_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3762097.1"
                     /translation="MRAGVDLGGTKIQAVVRERGATLLGEARRETPRTGGAEAVLDEI
                     AAAVREAATGAQLDVSGLQGVGVGAPGNVDQEAGTVEQVSNIDGWVEPYDLGPALADD
                     LGIPVVIGNDVNVAVEAERRFGAGRPFESFLGVFWGTGVGGGIVMNGRTVTGRGSAGE
                     IGHVCAKPGGRRCGCGLKGCVEAYAGRGALEERARAIAKKHDTILFELAEERGRDRLT
                     SGIWARALDAGDDVARGLIEKAVQALGVGIGSAVTLLDVEAVVVGGGLGERLGEDWLR
                     RIEEAARPHTFFAEPPLYVLAELGDYAGAIGASLLVR"
     gene            3434..4162
                     /locus_tag="AC150V_03910"
     CDS             3434..4162
                     /locus_tag="AC150V_03910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762098.1"
                     /translation="MAGTAIVTGAARGLGEAIARRLHRDGYAVGLGDVNAGGVEALAG
                     ELGERALPLALDVRSLESWEHSLETAVATFGAVDALVNNAARTEIRSLWEIDVDEWDD
                     VLATNLRGTYLGIRVVGAHLRDRGSGRIVNLASVAGQNNRAVTGVHYASSKAAIVALT
                     RHAATELAPHGVTVNALAPAAIDGPMVASVPPERIEAMVQTIPVRRLGRPEEVAAAVA
                     YLLSDEAGFVTGATLDLNGGMLMR"
BASE COUNT          649 a         1446 c         1527 g          629 t
ORIGIN      
        1 tcccccacga cccagtcgcc cggctcgata cgcacgcgac cgatcgtgat cggcgcctgg
       61 gtcgactcga cctgccagcg ccaggtcgag tcctccgggg tcgtgaagcg ggtgaagacg
      121 gggaagcctt cgtcgacgat gaaccggaca tcgcgacacc cgccgtcgag cacgcatccg
      181 gcgacgccgc ggctgagcag cgaggtcgcg gacagctcac cgaagtgagc ggcgatgtcc
      241 tggtggcagg cgtacacggc cacatgcccg gacggcacgc tgcccagcat cgccagggtc
      301 ttgcggatgg ccgcgtccca gtcctgagtg tcggtcggac gcccctgaac ggtgaacgcc
      361 tgacccgcga ggcgcatccc cgggacgagc gggcggatct cgggtggaag cgtctggtgc
      421 cggtagccga gggcgtcgag gacgtccgtc aggacagcgg tgtagatgcg ctcgaagcgg
      481 tctctcatgg cattggaatc gcgaggtcgc cgggggtgtc gtcgccccgg ctctcgacga
      541 ggtcgggggc ccgctcgcga agcagtcgaa gcacgagctc ggtgcgacga acgcgctcgc
      601 gacccagctt cgcggcggtg tccgccatgt ggaggcccac gggatagatg ttcggcgcgt
      661 tccggactcc gagcttcacg tacatcggcg cggccagccg gatcatctct gccacctcgt
      721 agaaccgcac gaagccgccc tggtcgtcgg gcgcctcgac gtagacgtcg agcggcgcgt
      781 cgcaggccgc gcgcagctct ccgagctcgg caggggaaag atccgtcgag acgttgatcg
      841 tccctgcgcc cagcgattcg agggtcgctg cggtcacggg gttcgcgcag gggaggagta
      901 ccgaggtctt gagcacgagg ctctccggga gctcgccgcg tcgcttcaat tcgcccagcc
      961 gggcgaggac gccgatatcg gcgacgagga acgatctgat gccgagggag aggccccgtt
     1021 gcacctccgc cacgcaccac tcgacgccgt cggagccgcg cgcaacgccg ccgacagccg
     1081 aagtcgcgag cgactgaccg cccgcatccc acgcgccgcg gggcccgagg aacagcgaca
     1141 cctcgatgcc gtgctcggcg ccgagagcgg ccatctcggc gatctcggcg tccgtcagca
     1201 tcatcacgcc ggagccctgc gagacacgcc gcacggggac gtcccgggct tgcgcctcgt
     1261 cgagcacggc acggaagacc gcgggacctt ccaccgacgg gatctcgacc cggtagcgaa
     1321 ggccgtcggc gaaacgcagc tcgctcatcg ggcgagctta ctaagcgcgc tacgcccaga
     1381 actcgtcgaa cgcggcggga ttcgcgggga gcgcgagaac cgtccgcacc agcccgccct
     1441 cgaagccgac gagcaggacc tggtcgatgt cgaggctgcg tccgcgccgc tcgccgaaag
     1501 cgcggtacaa cgcggctgcg cgcttctccg agaccaggac atcgacaaga cgcgagccgt
     1561 acgtgccgcc cgtctccttg ggcagccgcg ccaagaagcg gatgatctcc tcgcgccccg
     1621 tatacgtgcc tgccattacg ccctcgccgg gcactaccca ctcggcgtcc ggtgcgaaca
     1681 cctcgcgcaa cgcgaacccg tctcttctgg cgaacgcttc gaagagcccg cgcacgacgc
     1741 gttcgttctc agccgtcccc actcgactct tcccaccggt cgatcaaccc gtcgacgtgg
     1801 cgccgttgct cgtcggtgaa gtcgaagtgc ctgagcagtc ggcgcacctc gtccggatcg
     1861 tcgttctcga ctgcggcctc gaggtgttcc cgcatcggcg tcggctgcct gagctgacgc
     1921 cggagccagc gaagcaggct gacagttgcc tggtcggtca tgcgtcgtca ggctatcccg
     1981 atggacacct tccttgcgat cgccagccgc cgagacgagc ggagctatac gagcgacccg
     2041 ttacccgatg gggtggtggc acgcatcctc gacgcggggc ggttgagcgg ctcgtcgaag
     2101 aaccgtcagg cgtgggtctt cgtcgttctc gacgaccgac gccgtatcga gcagctcgcg
     2161 gctgccgtgt acgtgcccga gaacgtcctg tcggcgggct tggtcgtcgc gatcctcgtc
     2221 tccggcaagg gtccgacctc cttcgacgcc gggagggccg cgcagaacat gcttctcgcc
     2281 gcctggaacg agggagtcag ctcgtgtccc aacgggattg ccgattccga cgctgctgca
     2341 gcggcactcg aggccggcga cgacgagcgg ccggtgatcg tcctcacctt cggcgcgccg
     2401 cgacgtccac gcgacgccga gagccgtgac gcctcggaat ggagcgcgcg cgcgaaccgc
     2461 aaaccggtcg acgaggtggt tcggaggctc gcttgagggc gggggtcgat ctcggcggca
     2521 cgaagatcca ggccgtcgtc agggagcggg gagccacgct gctcggcgag gcccggcgtg
     2581 agacgccgcg gacgggcggc gccgaggcgg ttctcgacga gatcgccgcc gcagtgcgcg
     2641 aggccgcgac cggtgcgcag ctcgatgtct caggcctcca gggggtgggc gtcggcgctc
     2701 cgggtaacgt ggaccaggaa gcgggcacgg tcgagcaggt ctcgaacatc gacgggtggg
     2761 tcgagccgta cgacctcggg ccggcgctcg ccgacgacct cggcattccg gtcgtgatcg
     2821 gcaacgacgt caacgtcgcc gtcgaagccg agcgccgctt cggagcagga cgcccgttcg
     2881 agtcgtttct cggtgtgttc tggggcaccg gcgtgggcgg ggggatcgtg atgaacggcc
     2941 gcaccgtgac cggccgagga tctgccgggg agatcggcca cgtctgcgcc aagccgggag
     3001 ggaggcgctg cggctgcggg ctcaaggggt gcgtcgaggc gtacgcggga cggggagccc
     3061 tggaggagag ggctcgggcc atcgccaaga agcacgacac gattcttttc gagctggccg
     3121 aggagcgcgg ccgcgaccgc ctcacgagcg gcatctgggc gcgggcgctg gacgccggcg
     3181 acgacgttgc gcggggtctg atcgagaagg ccgttcaggc gctcggcgtc ggcatcggct
     3241 cggccgtcac tctgctcgac gtcgaggcgg tcgtcgtcgg gggcggactg ggcgagcgtc
     3301 tcggcgagga ctggctgcgc aggatcgagg aggccgcccg gcctcacacg tttttcgcgg
     3361 aacctccgct ctacgtcctc gcggagctcg gagactacgc gggcgcgatc ggcgcgagcc
     3421 tgctcgttcg ctgatggccg gcacggcgat cgtcaccggc gcggcacgag gtctcggaga
     3481 ggcgatcgcg cgccggttgc acagggacgg ctacgcggtc gggctcggcg acgtgaacgc
     3541 cggcggcgtc gaggcacttg cgggcgagct cggcgaacgt gctctgccgc tcgcgctgga
     3601 cgtccgctcg ctcgagtcgt gggagcattc gctcgagact gcggtcgcaa cgttcggagc
     3661 ggtcgacgcg ctcgtgaaca acgcggcccg aaccgagatc cgttctctct gggagatcga
     3721 cgtcgatgag tgggacgacg tgttggcgac caacctacgc ggcacgtacc tcgggatccg
     3781 ggtcgtcggt gcgcacctgc gcgaccgggg aagcggtcgc atcgtcaacc tcgcctccgt
     3841 cgccggtcag aacaaccgcg ctgtcaccgg cgtccactat gcgtcttcca aggctgcgat
     3901 cgtcgcgctg acccgccacg cggccaccga gctcgcaccg cacggcgtca cggtcaatgc
     3961 gctcgccccg gcggcgatcg acggtccgat ggtcgcgagc gtgccgccgg agcgaatcga
     4021 ggccatggtg cagacgattc ccgtgcgacg gctcggccgg cccgaggagg ttgcagcggc
     4081 ggtcgcctat ctgctctcgg acgaggccgg tttcgtcacc ggggcgacgc tggatctcaa
     4141 cggcggcatg ctgatgcgct gagctcgtcg gtagcgcccc tgatcctgtc tgcggaattc
     4201 cggactcgta accccgtgat cctccgatgc gcgcacgccg gtcgcctgcg a
//
LOCUS       DBNGNK010000134         4767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1885866,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000134 DBNGNK010000000
VERSION     DBNGNK010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1885866"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..466
                     /locus_tag="AC150V_03915"
     CDS             137..466
                     /locus_tag="AC150V_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR3762099.1"
                     /translation="MPIYEYRCPNGHTFEVFQRMSDDPVESCETCGAGPVEKVLFPVA
                     VHFKGSGFYSTDYGKAKKGSGGKEADASSEKSGSKEAGGEGGAKDSGKDSGKDSSSGE
                     KKTKSGD"
     gene            complement(478..1632)
                     /locus_tag="AC150V_03920"
     CDS             complement(478..1632)
                     /locus_tag="AC150V_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3762100.1"
                     /translation="MPEQTKPYRRYKGGRVKGRVPLERASAQTPGSAPPRRRRYRPRR
                     IGLWLAGGLLLIAVLMLAWLGTSYLAFKSGIDEANARVPESVAAQLAEQEGLLTSTPT
                     TILVVGTDGSTRGGRNDDRRSDSLMLIHTDPRKHTLSFLSIPRDLRVDVPGYGAEKIN
                     SAFQFGGATLALQTVKNLTGLEINHVVFVDFDRFEEVIDAVGGIDVDVEKPIVSNSFD
                     CPLDTNSECAQWEGWRFSKGTQHMDGQRALVYSRVRSNQLDPGETDFTRARRQQQVVQ
                     ATADRLTSVGTAWRLPFIGDDVVAPLATDLSAGQVLQLGWVYFRADPGKALHCRLGGE
                     PATVSGESVILGSEDNVATIAMFEGKSAPLPPQKRLPYAPGCVVGNLPSK"
     gene            1656..2564
                     /locus_tag="AC150V_03925"
     CDS             1656..2564
                     /locus_tag="AC150V_03925"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR3762101.1"
                     /translation="MLRHPAIGVDVGGTKILAGLVSRDGEIGQTVEIHTPTDSKDAIV
                     EAIAALAEQLLAKESAEAIGLGVPCNLDASTGACYRATNLPLGEVRFKERLGEHVGLP
                     VGVENDGNAAALAEWRLGAGRGADDLVMLTLGTGVGGGLVLDGKLYRGWAELGHVVVD
                     EGGPPCQGNCHGRGHLETEASGHAADRAARELYGNGASAVDLVERATAGEAEAVAALA
                     HIGTLLGSAVGSFVNVFDPDVVVIGGGFGAAAGDLILEPARAAARREAIHPADERLRI
                     VEAELHEQAGLIGAGLVGFEALDGLR"
     gene            2568..3377
                     /gene="rsmI"
                     /locus_tag="AC150V_03930"
     CDS             2568..3377
                     /gene="rsmI"
                     /locus_tag="AC150V_03930"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR3762102.1"
                     /translation="MPLAVCATPIGNLDDVTLRVLTELREADVVLCEDTRRTRGLLDR
                     HGIEAQRLLSYHRHSEARRVAQLLPLLDEGARVALVSDSGMPGVNDPGARLVSAAIEV
                     GVPVTVLPGPSAVETALVASGLLGEEYRFLGYLPRREAQRVALWDVLAGWSHPAVAFE
                     APTRLPASLASLAAAQPDRSVAICRELTKLHEDVARGTAAELAARFTEAPRGEVTIVL
                     GPAESEPSDGVDEALAAVEELVASGATRRVAAAAVARLTGVARNTLYRGSL"
     gene            3474..>4767
                     /locus_tag="AC150V_03935"
     CDS             3474..>4767
                     /locus_tag="AC150V_03935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3762103.1"
                     /translation="MRGAVVVIGALVALTVASAAGAWAWPLDGPVLRPFAFGADPYAA
                     GLHRGIDIGGVEGEPVRAPAGGTVTFAGSVPASGRVVTIATADGYAVTLTHLGSVAVT
                     EGESVAEGAVVATVGASGDAEWPSPYVHFGVRSVADPHGYLDPLAFLPPRPSPQAPEP
                     AAPPMPEAAPTPVPTPPPPATADRIAPPKAVVPPPPQVPLLPEPATPSVEPGPEPIQV
                     ALPVPVPEQAAPPQPSTPPEPAAQAQVVAAVPTRASAPAAPAPIRTPDPLRSPGVPQI
                     PTPAAVALGEEAAPAWAGRAAAADRPSPPATQEAWRSSPPSAATTPTTTPSAGRVDTG
                     RAIETAQRPDPATPSARATSPAPTGGDLADPPETKPAVAAPPPPSLRVPIGRAGPTGV
                     GVVKLPRGGERLLPAVPSESPVSVARPRGSLRASHGNES"
BASE COUNT          691 a         1649 c         1708 g          719 t
ORIGIN      
        1 cgccggcgcc gccggcgccg agacacaccg atgggcctcg ctgccttcga gagcgccccg
       61 tgctaccagg agctgaacga cccggaccgc tctggagacg ggtgacgatc gtggcagccg
      121 ggtaggctgc tccggcatgc cgatctatga gtaccgctgc ccgaacgggc acacgttcga
      181 ggtcttccag cgcatgagcg acgaccctgt ggagagctgc gagacgtgcg gcgcggggcc
      241 cgtcgagaag gtgctctttc ctgtcgcggt tcacttcaag ggctccggtt tctacagcac
      301 cgactacggc aaggccaaga aggggtcggg cggcaaggag gcggacgcct cgtcggagaa
      361 gagcggctcg aaggaggccg gaggcgaggg tggcgccaag gattccggca aggattccgg
      421 caaggactcg agctcgggcg agaagaagac gaagtccggc gactaggcgt cggctcccta
      481 cttcgacggc aggttgccga ccacgcagcc cggggcgtac ggcagcctct tctgcggcgg
      541 cagaggggca gacttcccct cgaacatcgc gatcgtcgcg acgttgtcct cggagcccag
      601 gatcacggac tcgccgctca cggtcgcggg ctcgccgccg agccggcagt ggagcgcctt
      661 gcccggatcg gctcggaagt agacccagcc gagctgtagt acctggccgg cgctgagatc
      721 cgtcgccagg ggggcgacga cgtcgtcgcc gatgaagggc aatcgccacg ccgttccgac
      781 gctcgtcagc ctgtcggccg tggcctggac gacctgctgc tggcgacgcg ccctcgtgaa
      841 gtcggtctcg ccgggatcga gctggttcga gcgcacgcgc gagtagacga gcgcccgctg
      901 accgtccatg tgctgcgttc ccttcgagaa tcgccagccc tcccattgag cgcactcgga
      961 gttcgtgtcg agagggcagt cgaacgagtt cgagacgatc ggcttctcga catcgacgtc
     1021 gatgccgccg accgcgtcga tcacttcctc gaagcgatcg aagtcgacga agaccacgtg
     1081 gttgatctcg aggccggtca ggttcttcac cgtctgcaat gcgagcgtcg ccccgccgaa
     1141 ctggaacgcg ctgttgatct tctcggctcc atagccgggg acgtccaccc gcaggtcgcg
     1201 cggaatcgag aggaaggaga gcgtgtgctt gcgcgggtcg gtgtggatca gcatcagcga
     1261 gtccgaccgg cggtcgtcgt tccgtccgcc acgcgtcgag ccgtctgtgc ccacgaccag
     1321 gatcgtcgtc ggcgtcgacg tgagcagtcc ctcctgttcg gcgagctggg ccgccacgct
     1381 ctccggcacg cgggcattcg cctcgtcgat tcccgacttg aacgcgaggt agctcgttcc
     1441 gagccacgcg agcatcagga cggcgatcag aagcaggccc ccggcgagcc atagcccgat
     1501 tcgtcgagga cggtaccgtc ggcgccgcgg tggagcgctc ccgggggtct gcgccgacgc
     1561 gcgctcgagc ggaaccctgc ccttgacccg cccgcccttg taacggcgat acggcttcgt
     1621 ttgctcgggc atcgggcggt tatcgtagcc aggtcgtgtt acgtcaccca gccatcggag
     1681 tcgacgtcgg cgggacgaag attctcgccg gactcgtctc gcgcgacggg gagatcggtc
     1741 agaccgtcga gattcacacg ccgaccgact ccaaggacgc gatcgtcgag gcgatcgccg
     1801 cgctggccga gcagttgctc gcaaaagagt ctgccgaagc gatcggcctg ggagttccct
     1861 gcaatctcga tgccagcacg ggcgcctgtt accgggcgac caatctgccg ctcggcgagg
     1921 tccgcttcaa ggagcgcctg ggtgagcacg tgggccttcc tgtcggtgtc gagaacgacg
     1981 gcaacgcggc ggcgctcgcg gagtggcggt tgggcgcggg tcgtggagcc gatgacctcg
     2041 tgatgctcac gctgggcaca ggtgtcggcg gcggtctggt tctcgacggg aagctgtacc
     2101 ggggctgggc ggagctcggc catgtcgtcg tcgacgaggg tggaccgccg tgccagggga
     2161 attgtcacgg ccgtggccat ctcgagacag aggcctccgg tcacgccgcc gaccgcgcgg
     2221 cgcgggagct gtacgggaac ggcgccagcg ccgtcgacct cgtcgagcgc gcgacagcgg
     2281 gagaggcgga ggcggtggct gcgctcgcgc acatcggaac actgctcggt tcggcagtgg
     2341 gctcgttcgt caacgtcttc gatccagacg tcgtcgtgat cggaggcggt ttcggcgccg
     2401 ccgcgggcga cctgattctc gagccggcgc gagcggccgc gcgccgggaa gcgattcacc
     2461 cggctgacga gcgactgcgg atcgtggagg ccgagttgca cgagcaggcg gggctgatcg
     2521 gtgcagggct cgtcggcttc gaggcactag acgggctccg gtagcccgtg ccactcgcag
     2581 tctgcgcgac cccgatcgga aacctcgacg acgtcaccct gcgcgtcctc accgagctgc
     2641 gggaggccga cgtcgtgctc tgcgaggaca cccgtcgcac ccgtgggctc ctcgaccggc
     2701 acggtataga ggcgcagcgc ctgctcagct accaccggca cagcgaggca cgcagggtcg
     2761 cgcagctgct cccgttgctg gacgagggag cgcgagtcgc gctcgtctcc gattcgggca
     2821 tgcccggcgt caacgacccg ggcgcccggc tcgtctcggc ggcgatcgag gtaggagttc
     2881 cggtcaccgt gctgcccggt ccgtcggcgg tggagacggc gctcgtcgcc agcggactgc
     2941 tcggtgagga gtatcgcttt ctcggctacc tgcctcgccg cgaggcgcag cgggtggccc
     3001 tgtgggacgt gctggccggc tggtcgcacc ccgcggtcgc cttcgaggct ccgacgcgac
     3061 tgcccgcctc gctcgccagt ctggccgccg cgcagccgga tcggtcggtc gcgatctgcc
     3121 gggagttgac caagcttcac gaagacgtag cgcgcggcac cgcggccgag ctcgccgctc
     3181 gcttcacgga ggcaccgcgg ggcgaggtca cgatcgtgct cggacccgcc gagtcggagc
     3241 cgagcgacgg agttgacgag gcactggcgg ccgttgagga gctcgtggcc tcaggagcta
     3301 cccgccgggt cgcagcggct gccgtcgcgc ggctgaccgg cgtcgcgcga aacacgctct
     3361 accgcggctc tctgtagtcc gattggacaa cagccgacgc tgccgctacc gtgtctcggt
     3421 cagttgtcaa ttcagagaac actgtccctt tcgtcaggga aaggagccgt cacatgcgag
     3481 gagccgtggt cgtgattggt gcattggtcg ccctcacggt cgcttctgcg gccggggcgt
     3541 gggcatggcc gctcgacggg cccgtgctga ggccgttcgc cttcggcgcg gatccgtatg
     3601 ccgccgggct gcatcgcgga atcgacatcg gtggcgtcga gggggaaccc gtcagggctc
     3661 cggccggcgg cacggtgacg tttgccggct cggtcccggc gagcggaagg gtcgtgacga
     3721 tcgcgaccgc cgatggctat gcggtcacgc tcacacacct cggttccgtc gcagtgaccg
     3781 agggggagtc ggtcgcggag ggggcggtcg tcgccaccgt cggcgcgagc ggagacgcgg
     3841 agtggcccag cccctacgtc catttcggcg ttcgcagtgt ggccgatccc cacggatacc
     3901 tcgacccgct tgcgttcttg ccgccgcgac cgtcgccgca ggcgccggag ccggcagcgc
     3961 cgcccatgcc cgaagctgct ccgaccccgg tgccgacccc gcctccgccc gcgacggctg
     4021 accgtatcgc tccccccaag gcggtcgtgc ctcctccgcc gcaagtgccg ctgctgccag
     4081 agccggcgac gccgtcggtg gagccgggac ccgagccgat ccaggtcgcg ctgccggtcc
     4141 ccgtccccga gcaggcagcg ccgccgcaac cttccacgcc gccggaacca gcggcgcagg
     4201 cgcaggtagt cgccgcggtg ccgaccaggg cgtcggcgcc cgcggctcct gcgccgatcc
     4261 gtactccgga tccactccga tcgccggggg tcccgcaaat cccgacgccc gctgccgtgg
     4321 cgctcgggga ggaggcggca ccggcctggg ccgggagggc ggcggcagct gatcggccca
     4381 gtccgcctgc gacacaggag gcatggcgtt ccagcccgcc gtctgcagcg acgacgccga
     4441 ccacgactcc ctcagccggg cgcgtcgaca ccggccgtgc gatcgagacg gcgcagcggc
     4501 ccgatcccgc cactccgtct gcgcgcgcaa cgtcgcccgc cccgacgggc ggcgacctcg
     4561 ccgacccacc ggaaacgaag ccagccgttg cggcgccgcc gcccccgagt ctccgagtgc
     4621 ccatcggtcg tgccgggccg accggggtcg gcgtcgtcaa gctccctcgc ggcggggaac
     4681 ggctgctgcc cgccgtgccg tcggagtccc cggtatctgt ggcccgtcca cggggaagtc
     4741 ttcgcgcatc acacggaaac gagtccg
//
LOCUS       DBNGNK010000135         5196 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1599425,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000135 DBNGNK010000000
VERSION     DBNGNK010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1599425"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="AC150V_03940"
     CDS             complement(<1..1421)
                     /locus_tag="AC150V_03940"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3762104.1"
                     /translation="MHLKSIRLRGFKSFPDTVELALEPGVAVVVGPNGSGKSNVADAI
                     VWAAGSLTPSELRAEKPDDVLFAGGGDRRGADHCEVELLFENEGGRFGELDFSEVSIM
                     RRLSRGGEGQYLVNRAPVRRTDLVELLADVGLSGSMHSVVGQGKVDAVLASKPADRRA
                     LVEEAAGLGKFKRRKHRAELKLARVATQVERARDVEDEVRKRIRPLALQASAAERAEK
                     LAVEIAALRARIAELDLAAIDERRAVADDRRRAATLARRTAQDKLSALLAERERAEEE
                     LSDAAGKREGALGALYRLQGACERLAIRQESSASLQARLRDDLAEAERALKAQSDEAA
                     GTLERAASAAAAAAREAAQESGQRAERARIAHERLAAHERQGARFAEQRLDELRRERE
                     EVEAGLAGETGGSEGANRTLLRLGALRERLTARRESMGQVAKQIRDDSAAARELAKSG
                     GVSPAELARRADDAVAAARAAAT"
     gene            complement(1465..2085)
                     /gene="rnc"
                     /locus_tag="AC150V_03945"
     CDS             complement(1465..2085)
                     /gene="rnc"
                     /locus_tag="AC150V_03945"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR3762105.1"
                     /translation="MHTSWAPDRTSSYERLEFLGDSVLELAIARALYDRFPEASEGRL
                     AKIRAHVVSRQSCAGVAKELELGAKLSGAAKDVAPGELKRISRSTNVLAALLEAAIAA
                     VYLEFGFEKVEPAIVEAFGGRIDFARTSHVDNKTELQEALARTGRQVSYSVLEVDGPP
                     HDRRFVCAALVDGEQIGVGSGSTKKAAEQEAAEQVLAALGITPQQL"
     gene            complement(2162..2407)
                     /gene="acpP"
                     /locus_tag="AC150V_03950"
     CDS             complement(2162..2407)
                     /gene="acpP"
                     /locus_tag="AC150V_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR3762106.1"
                     /translation="MATSRDEIFVRVKETLSEQLGVEETEITEDSSFQDDLEADSLDL
                     VELIMELEDQFGIKIPDEDAQKITTVGQAVDYVNTHQ"
     gene            complement(2455..3426)
                     /gene="plsX"
                     /locus_tag="AC150V_03955"
     CDS             complement(2455..3426)
                     /gene="plsX"
                     /locus_tag="AC150V_03955"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR3762107.1"
                     /translation="MTVTVAVDALGGDRAPGEIVAGALDAASDGIRPVLYGTAGLEAG
                     GLELVATDDVIAMDEKPADAVRAKPESSLVRAVRAVGAGQADAVVSAGNTGAMLAASL
                     LYIRRLPGVARPGIAVVIPSAQGPTVLIDAGANAEGRPEHLLQYAHMGAVFAEEVLGV
                     SAPEVRLLSIGEEPDKGNQLTREAHALLAYSGLRFGGNVEGRHLLDGSGDVVVADGFN
                     GNIALKTLEGAIRSVLEGLRSEIESSARGRLGGALIRPAARRLAGRLDAENTGGGYLL
                     GLQGLVVIAHGSSSRRAIASAIRLAARGVEHRITDVLADRLPDRVVT"
     gene            complement(3453..3641)
                     /gene="rpmF"
                     /locus_tag="AC150V_03960"
     CDS             complement(3453..3641)
                     /gene="rpmF"
                     /locus_tag="AC150V_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013908.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR3762108.1"
                     /translation="MAVPKRKTSRSRRDKRRAQHGIESPRLSVCSNCGQPKRPHRVCP
                     TCKTYGGREVEPLRAPAP"
     gene            complement(3693..4202)
                     /locus_tag="AC150V_03965"
     CDS             complement(3693..4202)
                     /locus_tag="AC150V_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR3762109.1"
                     /translation="MTRFPLRNLTLRPGEEHREEVALEIEPFELGGERYLNVPAAAPA
                     SLTIARATSGDVFGLSFVGRLLGPCMRCLAEAAVDVPIEVREYQDADPEAAEDLRTEY
                     VVDDQLELSLWARDAVALALPDQILCRDDCAGLCAVCGRDLNVEPHEHEEENADPRWA
                     ALEQLRDEL"
     gene            4315..>5196
                     /locus_tag="AC150V_03970"
     CDS             4315..>5196
                     /locus_tag="AC150V_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762110.1"
                     /translation="MNRTPTGRTAELRRPAILLLAGLVVGGAALLAALGLGASSGTAA
                     QSQYAPKNTTAPSLTGILTASQTLTATNGPWTGTPPITFTYQGLRCGADGKNCADIVG
                     ATARTYKLIADDVGRRVAVRVRATNAQGATNATSTPSALVQEGGPTGQIELPSGEKSI
                     PATSVPAEERLIVSAVQFSPNVISSRTEPIRILVTVKDTRGFVVRDVRVFVRSTPLVT
                     TTPPRQKTGQDGRITYTVLPEPDFPAIRNGTNVQYFVKAYRDGDPPLAGIAGYRLVQV
                     PFVAVKVWPAVRIPVREG"
BASE COUNT          762 a         1886 c         1743 g          805 t
ORIGIN      
        1 tccgtcgcgg ccgcccgcgc cgccgcgacg gcgtcgtcgg cccgtcgagc gagctcggcg
       61 ggcgagacgc ctccgctctt cgccagctca cgggcggcgg cggaatcgtc tctgatctgc
      121 ttcgcgacct ggcccatgga ctcgcgccgg gccgtgagcc gctcccgtag ggcgccgagc
      181 cggagaagcg tgcggttcgc gccctcgcta ccaccggtct ctccggcaag tccggcctcg
      241 acctcctccc gctctcggcg aagctcgtcg aggcgctgct cggcgaaacg ggcgccctgc
      301 cgctcgtgcg ccgcgagccg ctcgtgggcg atccgggccc gctcggctcg ctgaccgctc
      361 tcctgcgcgg cctcgcgagc cgcggcggcc gcagctgatg ccgcccgctc cagcgtcccg
      421 gcggcctcgt cgctctgcgc cttcaatgcc cgctccgcct cggcgaggtc gtcgcgcagc
      481 cgcgcctgca gactcgccga cgactcctgt ctgatggcaa gccgctcgca ggctccctgg
      541 agccggtaca gagcaccgag ggccccttcg cgcttgccgg cggcgtcgga gagctcctcc
      601 tcggcacgct cgcgttccgc cagcagcgcg gagagcttgt cctgagccgt acgcctggcc
      661 aacgtggccg ccctacggcg gtcgtcggca acggcccgcc gttcgtcgat cgcggcgagg
      721 tcgagctcgg cgatgcgcgc gcggagagcc gcgatctcga cggcgagctt ctcggcccgc
      781 tcagcggcgg aggcttgcag cgcgagtggc cggatgcgtt tgcgcacctc gtcctcgacg
      841 tcgcgggcgc gctcgacctg cgtggcgaca cgcgcgagct tgagctcggc gcgatgcttg
      901 cgtcgcttga acttccccag ccctgcggct tcttcgacga gcgcacgtcg gtcggccggc
      961 ttggacgcga gcaccgcgtc gaccttgcct tgacccacga cggaatgcat ggagccgctg
     1021 agcccgacgt ccgccagcag ctcgaccaag tcggtccgcc gcaccggtgc tcggttgaca
     1081 aggtactggc cctcgccgcc cctgctcagg cggcgcatga tcgagacctc gctgaagtcg
     1141 agctcgccga agcgccctcc ctcgttctcg aacaggagct cgacctcgca gtgatcggct
     1201 ccgcgcctgt ctccgccgcc ggcgaagagc acatcgtcgg gcttctccgc ccggagctcg
     1261 ctgggggtca gcgagcccgc cgcccagacg atcgcgtccg cgacgttcga cttcccggag
     1321 ccgttcggac cgaccacgac cgccactccc ggctcgagcg ccagctcgac cgtgtcgggg
     1381 aacgacttaa agccccggag cctgatcgac ttcaggtgca ccggggcgaa aagctacttg
     1441 tcgccccgga cgtgccaccg ccgactacag ctgctgcggc gtgattccga gggccgccag
     1501 cacttgctcg gccgcctcct gctcggcggc cttcttcgtg ctcccgcttc cgaccccgat
     1561 ctgctctccg tccacgagcg cggcgcacac gaagcgccgg tcgtgcgggg gaccgtcgac
     1621 ctccagcacc gagtaggaga cctgccgtcc tgtccgggcg agcgcctcct gcagctccgt
     1681 cttgttgtcg acgtggctcg tgcgcgcgaa gtcgatccgg ccgccgaacg cctcgacgat
     1741 cgcgggctcg accttctcga acccgaactc gaggtacaca gccgcgattg cagcctccag
     1801 cagggcggcg agcacgttgg tgctgcggct gattcgtttc agctcgccgg gcgcgacgtc
     1861 cttcgcggcc ccggagagct tcgcgcccag ttcgagctcc ttcgcgactc ccgcgcagct
     1921 ctggcgggag acgacgtgtg cacggatctt ggcgagccgc ccctccgagg cctccggaaa
     1981 ccggtcgtag agcgcccggg cgatcgcgag ctccagcacg ctgtcgccca gaaactcgag
     2041 acgctcgtac gaggacgtgc ggtcgggcgc ccaggaggtg tgcatgaaca cctgctcgcg
     2101 ctgctcggca ggcagcgcgt cgatcagcgc ggcaaggggg tgcttcgtcg ctggtcggac
     2161 gctactgatg tgtgttgacg taatcgactg cctggccgac cgtcgtgatc ttctgggcat
     2221 cctcgtccgg gatcttgatc ccgaactggt cttccagttc catgatcaat tcgacgaggt
     2281 cgagcgagtc ggcttccagg tcgtcctgga acgacgagtc ctcggtgatt tcggtttcct
     2341 cgaccccgag ctgctcgctc agtgtctcct tcacgcgcac gaaaatctcg tcacgggacg
     2401 ttgccacgtc gtacctcact tgttgccgtc gggttcagga gcgcgcagac tctactacgt
     2461 gacgacgcga tcgggcagcc gatcggcgag cacgtcggtg attcggtgct cgacgcccct
     2521 cgccgcgagg cggatcgcac tggcgatcgc gcggcgcgag ctcgatccgt gcgcgatcac
     2581 cacgagcccc tgaagcccga gcagatagcc gccgccggtg ttctccgcgt ccagacgccc
     2641 ggccaggcgg cgcgccgcgg gccggatcag agcgcccccc agacgaccgc gtgccgatga
     2701 ctcgatctcg cttcgcaggc cctcgagtac cgaacggatc gctccttcga gcgtcttgag
     2761 cgcgatattg ccgttgaagc cgtccgcgac cacgacgtcg cccgatccgt cgaggaggtg
     2821 gcgaccttcg acgttcccac cgaagcggag gcccgaatag gccaacagcg cgtgcgcctc
     2881 gcgtgtcaac tgattgccct tgtcgggctc ctccccgatc gacaagagcc gcacttcggg
     2941 tgcggacaca ccgagcacct cctccgcgaa gacggctccc atgtgcgcgt actggagcag
     3001 atgctccggg cgcccttcgg cgttcgcgcc tgcatcgatg agcaccgtcg gaccctgcgc
     3061 cgacgggatc acgaccgcga tcccggggcg agcgacgccg ggcagccggc ggatgtagag
     3121 gagcgacgca gccagcatcg cgcccgtgtt gcccgcagac acgaccgcat cggcctgtcc
     3181 ggcgccgaca gcgcgaaccg cccggacgag cgacgactcc ggtttggcac gtacggcatc
     3241 cgcgggcttc tcgtccatcg cgatcacgtc gtcggtggcg acgagttcga gcccacctgc
     3301 ctccaggccg gcggtgccgt acagcacggg acgaatgccg tccgacgccg cgtcgagcgc
     3361 tccggccacg atctcgccgg gcgcccggtc gccgccaagc gcgtcaactg cgacggtcac
     3421 ggtcacccgg tgcccttcgt gtcgcgcgcc gtctagggag ccggagcccg caggggctcg
     3481 acctcgcgcc cgccgtaggt cttgcacgtt gggcagacgc gatgcggccg cttcggctgc
     3541 ccgcagttgg agcagacgct gagacgcgga gattcgatgc cgtgctgcgc ccggcgcttg
     3601 tcacgccggg agcgggaggt ctttcgcttc gggacagcca tcggcggctg cgcagtatag
     3661 ccgcgcgaat gcgcgccgac ggggccaggt agctagagct cgtcgcggag ctgctccaga
     3721 gccgcccagc gtggatcagc gttctcctct tcgtgctcgt gcggctcgac gttgaggtcg
     3781 cggccgcaga cggcacagag acccgcgcag tcgtcacggc agaggatctg atcgggcaag
     3841 gcgagtgcga cggcgtcccg agcccagagc gatagctcga gctgatcgtc gacgacgtac
     3901 tcggttcgga gatcctcggc cgcctcggga tcggcgtcct ggtattcccg cacctcgatc
     3961 ggcacgtcga cggctgcttc cgcgagacat cgcatgcagg ggccgaggag gcggccgacg
     4021 aaagagagcc caaagacatc accactcgtc gcccgtgcaa tcgtgagcga ggcgggtgcg
     4081 gccgccggaa cgttgaggta gcgctcgccg cccagctcga aaggctcgat ctcgagcgcg
     4141 acttcctcgc ggtgctcctc ccccggccga agcgtcaggt tgcggagagg gaagcgggtc
     4201 atgtgctgga gggtagcccg gccagggccg cgcacgtacg cgggagacgc acgcgccacg
     4261 acgggaggag acgttgagct tcgtcgcacc acacatgaaa ggagacacat cgagatgaac
     4321 aggacaccaa ccgggcgcac ggccgagttg cgccgccccg cgatcctcct gctggcggga
     4381 cttgtcgttg gcggcgccgc gctgctggcc gcactcgggc tgggagcctc gagcggcacc
     4441 gcggcacagt cgcagtacgc gcccaagaac acgacggctc cctccctcac cgggatcctc
     4501 accgcgagcc agaccttgac cgcgacgaac ggaccctgga ccggaacgcc gccgatcacg
     4561 ttcacctacc aggggcttcg ctgcggtgcg gacgggaaga attgcgccga catcgtcggc
     4621 gcgactgctc ggacgtacaa gctgatcgcc gacgacgtgg ggaggcgcgt cgcggtgcgc
     4681 gttcgcgcca ccaacgcgca gggggcgacg aacgccacgt cgactccatc cgcgctggtg
     4741 caggagggcg gcccgaccgg tcagatcgag ctgccgagcg gcgagaagtc gatccccgcg
     4801 acgagcgttc ccgcggaaga gcgcctgatc gtctccgctg tgcagttctc gccgaacgtg
     4861 atctcttcgc gcacggagcc gatccggatc ctcgtcaccg tgaaggacac acgcggcttc
     4921 gtcgtacgcg acgtgcgcgt gttcgtacgc tcgacgccgc tcgtcacgac cacgccaccc
     4981 cggcagaaga ccggccagga cggccgtatc acctacaccg tccttcccga gccggacttc
     5041 ccggcgatcc gcaacggcac caacgtccag tacttcgtga aggcctaccg ggacggggat
     5101 ccgccgctcg ccggcatcgc cggctaccgg ctcgtccagg ttccgttcgt cgcggtcaag
     5161 gtctggcccg cggtgaggat cccggtgagg gaggga
//
LOCUS       DBNGNK010000136         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1240028,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000136 DBNGNK010000000
VERSION     DBNGNK010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1240028"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1098)
                     /locus_tag="AC150V_03975"
     CDS             complement(121..1098)
                     /locus_tag="AC150V_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3762111.1"
                     /translation="MKLAVLSLRDLDRRIFWRNVAPPLEDALASLPGGTLVVPPALRR
                     GSLLASRPAWLNAIRTVRRADMVFWLQLHLRPPTPLWALAYLRPLAKRSVLAVDSFEP
                     AIPSLGRILDAQRVTVTFTVYSQAARALRLSHPHLSVKWLPLGFNRAGFRDLGIERDL
                     DVYWMGRRHEPLHAALLRHAESGAISYSYSRSPGDPKTTEELNRIISRSRYFVVTPPD
                     LDDPIRTGSFSPVTSRYLEGLGAGARLLGVSPRSGELERIVPDVELVQCAADGSDLAE
                     VLAKADADAGWERKRIAARDVVHAEHSWDQRAVTVHRQLIAAMETEAAT"
     gene            complement(1188..2645)
                     /locus_tag="AC150V_03980"
     CDS             complement(1188..2645)
                     /locus_tag="AC150V_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR3762112.1"
                     /translation="MKKRASAGVFVVASWGLANLLTAFFGNLVLARLLEPRDFGIVAV
                     GMTVMVLAGAFADGGLANGIIRRAEPPTPSELRTLTGISLLLMIAITAIVAVVAMQFG
                     LVGQVTALMILALPISGFQGAGRAVLTRDLRLKAVSAVDAAGVFAFYAWAIPSVVLGA
                     GLWGLASATVVRSLAGTIAVGVIPGAGLYRPSLENVREFGALLWFGARFQANWLVYVL
                     REQGLNAVTGITGGVYTLGIWSLASRLLLVPMLLYEALGRVTFPTMAHILGRGDDPAP
                     LVERTARLAAVASTVLLPAFVASAPGVVPSLFGEQWTAVPEAFPGAALGLLIGGTVGS
                     AAVGYLFAANRPGTVLAASVALAVAWIATAAALLPFIGVAALGIGWAVSAVAESIVLA
                     PATRRLCGARVVRPLAYTLPIAVAATAAGWLITELGSATLWSGLGGGALALLLSVAGL
                     AVFARGPFVDLIQIARNSVLDAVRRDRVSAVTEPA"
     gene            2913..>3028
                     /locus_tag="AC150V_03985"
     CDS             2913..>3028
                     /locus_tag="AC150V_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR3762113.1"
                     /translation="MDPSNRNAQLTAREHDVARLIALGRSNAEIASSLGVTI"
BASE COUNT          541 a         1007 c         1075 g          405 t
ORIGIN      
        1 aaggtgccgg gccccgccgt gttcggaccg tacgagctgc tcggtcgatg tgctcgagcg
       61 tcacgagcac gcccttgagt gacgcacaga acgcgatgaa cgccgtctcc ttgtgcctcc
      121 tcatgtcgcg gcctccgtct ccatcgcagc gatgagttga cggtggacgg tcacggcccg
      181 ctggtcccag ctgtgctcgg cgtgaacgac gtcgcgagcg gcaatgcgct tccgctccca
      241 gcccgcgtcg gcgtcggcct ttgcgagtac ctctgcgaga tcggagccgt cagctgcgca
      301 ttggacgagc tcgacgtcag ggacgatccg ctcgagctcc cctgagcgcg gcgagacacc
      361 gaggagtcgg gcgcccgctc caagaccctc caggtagcga gacgtcaccg gactgaagct
      421 gccggtgcga attgggtcgt ccaggtcagg cggggtcacg acgaagtacc tgctgcgcga
      481 gatgatgcgg ttgagctctt ccgtcgtctt gggatcgccg ggcgagcgcg agtacgagta
      541 cgagatcgcc cccgactcgg cgtggcgcag caacgccgcg tgcaggggct cgtgcctgcg
      601 gcccatccag tagacgtcca ggtcgcgttc gataccgagg tcgcggaagc cggcgcggtt
      661 gaagccgagc ggcagccact tcacggatag gtgcggatgc gacaggcgca aggcccgcgc
      721 cgcctgcgag tagaccgtga aagtgaccgt gacccgttgt gcgtcgagaa tgcgaccgag
      781 cgacggtatc gccggctcga aggagtcgac cgcaagcacg ctccgcttgg ccagcggacg
      841 gaggtaggcg agcgcccaga gcggagtcgg aggacgaagg tggagctgaa gccagaacac
      901 catgtccgcg cggcgcaccg ttcggatggc attgagccac gcgggacgcg acgccagcag
      961 gctgccgcgc cggagggccg gggggacaac gagggtccct ccgggtagcg aggcgagagc
     1021 gtcctcgagc gggggcgcaa cgttccgcca gaagatcctc cggtcgaggt cgcggagtga
     1081 gaggacggcg agcttcacga cgtttcacat tgtcggcgtc gatgccgaga gcgccagggg
     1141 agcaagtagc cgtgctcctg ccgccgcgcc acggtcgccg gggcggctca cgccggctcg
     1201 gtgacggcgc tcacccgatc tcgccgaact gcgtccagaa cagaattgcg ggcgatctgg
     1261 atcagatcga cgaacgggcc gcgggcaaag acggcgaggc ccgcgacaga gagcagcaac
     1321 gccaaggcgc ctccgccgag tcccgaccag agcgtggcgg agcccagctc ggtgatcagc
     1381 cagccggcgg cggtggcggc gaccgcgatc ggaagcgtgt acgccagtgg tcgaacgact
     1441 cgcgccccac acagccgacg cgttgccggc gccagaacaa tgctctctgc caccgcgctg
     1501 acggcccaac cgattccaag ggcggccacg ccgatgaagg gcagcagcgc tgcggctgtc
     1561 gcgatccagg ccacggcgag cgcgacggaa gcggcgagta ccgtcccggg ccggttcgcc
     1621 gcaaagagat agccgaccgc tgccgagccc accgtcccgc cgatgagaag cccgagcgcc
     1681 gcgccgggga aggcctcggg cactgcagtc cattgctcac cgaagagcga cggaacgacg
     1741 cccggagcgg acgcgacgaa cgcgggcagc agcacagtgg aggcgactgc ggcgagtcgt
     1801 gcggtgcgct cgaccaacgg cgctggatcg tcgccgcgac cgaggatgtg cgccatggtc
     1861 gggaaggtca ctcgcccgag cgcctcgtac agcagcatcg gaacgaggag caggcggctc
     1921 gccaaggacc agatccccag tgtgtagaca ccacccgtga tgcctgtcac cgcattgagg
     1981 ccctgctcgc ggagcacgta gacgagccag ttggcctgga agcgggcgcc gaaccagagc
     2041 aacgccccga actcccgcac gttctcgaga gatggccggt agagaccggc gccgggaatg
     2101 acgcccaccg cgatcgtccc ggccaggctc cgcacgaccg tggccgatgc cagcccccac
     2161 agaccggcgc ccaggacgac gctgggaatc gcccacgcgt agaacgcaaa gacccccgcc
     2221 gcatccacgg cgctgacggc cttgagccgg aggtctcgcg tgaggacggc tcggccggcg
     2281 ccttggaatc cggagatcgg gagcgccagg atcatgagtg ctgtcacctg gcctaccagg
     2341 ccgaattgca tcgccacgac cgcgacgatg gccgttatcg cgatcatcag cagcagcgag
     2401 atgccggtga gggttcgcag ctcgctcggg gtcggcggct ccgcgcgccg gatgatcccg
     2461 ttcgcgaggc cgccgtccgc gaaggcgccg gcgagcacca tcacggtcat tcccaccgca
     2521 acgatgccga aatcgcgcgg ctcgaggagc cgcgcgagaa cgaggttgcc gaagaacgca
     2581 gtgaggagat tggcgagacc ccacgaagca acgacgaaga cgccggcgga tgctcgcttc
     2641 ttcatctccc cgcgtgaaag cgactcgccg acggccgcgt cctcccgcgt gggttccgag
     2701 cgtgtgctcg gccggggctg ctcgtcttct gccatatctc actgacgcta cggcaggaag
     2761 gtgcacgctg ctccggtgcc gtggctagtg attcctagcc aaccggctag ggcggttcgc
     2821 agcaaggcta gccgcttggg agtggtggcg cggggcgtgc gctccctatc gtgcgggcat
     2881 gctcgaggtc ggcgacagcg gcgggggttt ccatggaccc gtccaatagg aacgcgcagc
     2941 tcacggcgag ggagcacgac gtcgccaggc tcatcgcgct cgggagatcg aacgcggaga
     3001 tcgcctcgtc tctcggcgtg acgatcca
//
LOCUS       DBNGNK010000137         5108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1886489,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000137 DBNGNK010000000
VERSION     DBNGNK010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1886489"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC150V_03990"
     CDS             <1..528
                     /locus_tag="AC150V_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR3762114.1"
                     /translation="MRICTDWLKGFYRRLGETGIHSISMNETWVGVGVAKEHFVEFMK
                     PYEAECVQAAHDAGYLVSFHNCGRATLLLEDIADTGPDAVETLTSDRSSGDVDLADAK
                     QRIGDRVCLFGGFNEHLLHEADEDAVREEVRRCLDAAMEGGGYVLRSTGQIFDAKPGS
                     IEAMCETARELGRYN"
     gene            525..2510
                     /locus_tag="AC150V_03995"
     CDS             525..2510
                     /locus_tag="AC150V_03995"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050401 - xylonate dehydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HIR3762115.1"
                     /translation="MSAALRDDELYDVETAADGPAGRLPLTEEFLRDSPSGDVFGLSQ
                     NAGMGWEAEKVGREQFLILSTQGGVRADDGTPVALGYHTGHWEVGLLVREAAKELDRL
                     GVLPFAAHVSDPCDGRTQGTPGMFDSLPYRNDAALVLRRLVRSLPNRRGVIGVGTCDK
                     GLPAMMMAVASFGDLAGAIVPGGVTLPPTQGEDAGTIQTVGARFAHELVTLDEAADLG
                     CRACATPGGGCQFLGTAATAQVVAEALGLTVPHAALAPSGQPIWLDAAARTARAVHGQ
                     WESGRTLDDVLTDAAVENAMLVHAAVGGSTNLLIHVPAIAHAAGLRRPTVDDWRRVND
                     AVPRLVDALPNGPENHPTVRVFLAGGVPEVMLHLRRLGLLDTTATTIEGRTWEELLDE
                     WEQSERRAVLRARLREADGVDPDDVICPPDRARAKGLTPTVCFPGGNLAPGGSVIKST
                     AIDRRVLDEDGVYRLVGPARVFTSERDVIAAIKGTAGNPIQAGDVIVLAGCGPMGTGM
                     EETYQVTSALKFLPFGSQVALVTDARFSGVSTGACIGHVSPEGLEGGPIGRLLDGDLV
                     SVVVDRNELSASVDLVGRGDDRWTSDEAARVLEQRQPRSDLAPAEGLPGDTALWARLQ
                     SVSGGLWGGCVYDHEAILRALETSVTAGVGGEERPLA"
     gene            2545..3558
                     /locus_tag="AC150V_04000"
     CDS             2545..3558
                     /locus_tag="AC150V_04000"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR3762116.1"
                     /translation="MKQATIRDVAREAGVSVATVSRALNGQPFVRPEVRDRVLAASRK
                     LGYRPDLAARSMRTGTTGAVGLVVSDISNPLFATIARAADETLSPLGYALMVANSAND
                     DEHESELISALSQRRLDGLIIATATERAPGLAERIGSFQSAVLLDREVPGARADAVLS
                     DHGTGLADAIRHLARLGHRRIALIAGTAEQRGGRVRIDRYHEELDALGLEHGNGLCRT
                     GELTAEDGYRALREVLTLADPPTAIIAGNNQVFAGLFAAIRDLGVRVPTDLSVIACEE
                     TELTALHNPPLDVVRRDLAELGREAASLLLTRLEAPRRRRRVVVLPSAFEARGSSAPP
                     PTR"
     gene            complement(3576..4679)
                     /locus_tag="AC150V_04005"
     CDS             complement(3576..4679)
                     /locus_tag="AC150V_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR3762117.1"
                     /translation="MPELFGRRYARRDLSRRVGSLDQVAGIRLVTLGDGGGRGVRVLE
                     FRTGTGFTFDVLVDRAFDIGRCELRGTPLAWTSAVGVQGPWFYEPEGLGFFRGFLGGL
                     LTTCGIEHALFMAEDTAAQYHYPPKQTETFGLHGRVSNRPARLAGYGEAWEGDECVLW
                     AEGETLQATVMGEQLMLRRRIEARVGESRLALHDVVENVGHDRTPHMLLYHVNTGFPV
                     VDEGSEILVAASGVEARGDHPVEGYRTLAAPEAGYVEQVFEHELAPEQRGTVPAAVVN
                     RELGVGAYEVFRQDQLPHHFVWRMLGEGTYVVGIEPSTNRTGGRLDARERGELIELEA
                     GESRRYDLELGALDGAAEIDAFAARCERAVGGG"
     gene            complement(4713..>5108)
                     /locus_tag="AC150V_04010"
     CDS             complement(4713..>5108)
                     /locus_tag="AC150V_04010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762118.1"
                     /translation="IVNIGSITGAVVGMPGRAPYAASKGGVVGLTRTLAVEWADRGVR
                     VNAVLPGPVRTPMVLEAIESGILDESEIIDRTPAGRLALPEDVAGAVVMLCISGAGFV
                     TGQAVSVDGGYSTYGAAHSASRRYESPPA"
BASE COUNT          745 a         1875 c         1770 g          718 t
ORIGIN      
        1 atgcggatct gcacggactg gctgaagggc ttctaccggc gcctcggcga gacgggcatc
       61 cactccatct cgatgaacga gacctgggtc ggcgtcggtg tcgcgaagga gcacttcgtc
      121 gagttcatga agccgtacga ggccgagtgc gtccaggctg ctcatgacgc cggctacctc
      181 gtcagcttcc acaactgcgg gcgcgccacg ctgctgctcg aggacatcgc cgacacgggc
      241 ccggacgcgg tcgaaacgct cacgtccgac cgctcctccg gcgacgtcga cctcgcggac
      301 gccaagcagc gcatcggcga ccgggtgtgc ctcttcggcg gcttcaacga gcacctcctg
      361 cacgaggccg acgaggacgc cgtgcgggag gaggtcaggc gctgcctcga cgcggcgatg
      421 gagggcggcg gctacgtcct ccgctctacc ggacagatct tcgacgcgaa gcccggctcg
      481 atcgaggcca tgtgcgagac ggcgcgtgag ctcggtcgtt acaactgagc gccgccctgc
      541 gcgacgacga gctctatgac gtcgagacgg ccgccgacgg cccggccggc cgcctgccgc
      601 tgaccgagga gttcctgcgc gacagcccgt cgggcgacgt cttcggtctc tcccagaacg
      661 ccgggatggg ctgggaagcc gagaaagtcg gccgcgagca gttcctgatc ctctcgactc
      721 agggcggcgt gcgggccgac gacgggacgc ctgtcgcgct cggctaccac accggccact
      781 gggaggtcgg tttgctcgtc cgcgaagcgg cgaaggagct cgaccggctc ggcgtgcttc
      841 cgttcgccgc ccacgtctcc gacccgtgcg acggcaggac gcaagggacg ccgggcatgt
      901 tcgactcgct cccctaccgc aacgacgcgg cgctcgttct gcgacgcctc gtccgcagcc
      961 tgcccaaccg tcgtggcgtc atcggcgtgg gtacctgcga caagggtttg cccgcgatga
     1021 tgatggcggt cgcctcgttc ggcgacctcg ccggcgcgat cgtcccgggc ggcgttacgc
     1081 tccccccgac gcaaggcgag gacgccggga cgatccagac ggtcggcgcg cgcttcgcac
     1141 acgagctcgt gacgctcgac gaggccgcgg atctcggctg ccgcgcctgc gccacgcccg
     1201 gcggcggctg ccagttcctc ggcacggccg ccaccgcaca ggtggtcgct gaggccttgg
     1261 gtctcactgt ccctcacgcg gccctcgcgc cctcgggcca gccgatctgg ctcgacgcgg
     1321 cggcacgcac ggcgcgcgcg gttcacggcc agtgggagag cggccgcacc ctcgacgacg
     1381 tgctcaccga tgcggccgtc gagaacgcga tgctcgtcca cgcggccgtc ggcggctcga
     1441 cgaacctcct gatccacgtg ccggcgatcg cgcatgccgc cggcctgcgc cggccgaccg
     1501 tcgacgactg gcgtcgcgtc aacgacgcgg ttccacgcct cgtcgacgcg ctgcccaacg
     1561 gcccggagaa ccacccgacg gtgcgcgtgt tcctcgccgg cggtgtgccc gaggtgatgc
     1621 tgcacctgcg ccgcctcggc ctgctcgaca cgaccgcgac gacgatcgag ggccgaacgt
     1681 gggaggaact gctcgacgag tgggagcaga gcgagcgcag agccgtcttg cgcgccaggc
     1741 tccgagaggc cgacggcgtc gatcccgacg acgtcatctg cccgcccgac cgcgcgcgcg
     1801 caaagggcct cacgccgacg gtctgcttcc ccggcgggaa cctggccccc ggcggcagcg
     1861 tgatcaagag cacggcgatc gaccggcgcg tcctcgacga ggacggcgtc taccgtcttg
     1921 tgggcccggc ccgcgttttc acgagcgaac gcgacgtgat cgccgcgatc aaggggaccg
     1981 cgggcaaccc gatccaagcg ggtgacgtga tcgttctcgc gggctgcggc ccgatgggca
     2041 cgggcatgga ggagacctac caagtcacgt cggcgctcaa gttcctcccc ttcggtagcc
     2101 aggtcgcgct ggtcacggat gcgcgcttct cgggcgtctc gaccggcgcc tgcatcgggc
     2161 acgtgagccc ggagggactc gagggcgggc cgatcggacg cctcctcgac ggcgacctcg
     2221 tcagcgtggt cgtcgaccgg aacgagctct ccgcctcagt cgacctcgtc ggccgcgggg
     2281 acgaccgctg gacgagcgac gaggcggccc gggtgctcga gcagcgccaa cctcgctccg
     2341 acctggcccc cgccgagggg ctgccaggcg acacggcgct gtgggcgcgc ctgcagtccg
     2401 tgagcggcgg gctgtggggc gggtgcgtct acgatcacga ggcgattctc cgggcgctcg
     2461 aaacgtccgt gacggccggc gtgggcggcg aggagcggcc actcgcttga caccccgccc
     2521 gcttcccgga aacgtttaca accggtgaag caggcaacga tccgcgacgt cgcccgcgag
     2581 gcaggggtct cggtcgcgac cgtgtcacgc gccttgaacg ggcagccttt cgtgcgcccg
     2641 gaagtccgcg accgggtgct cgctgcaagt cgaaagctcg gctaccggcc cgatctggcg
     2701 gcccgctcga tgcgcaccgg cacgacgggg gccgtcggtc tcgtcgtctc cgacatctcc
     2761 aaccctctgt tcgcgacgat cgcgagggcg gccgacgaga cgctcagccc gctcgggtac
     2821 gcactcatgg ttgcgaactc cgcgaacgac gacgagcacg agtccgagct catctccgcc
     2881 ctcagccagc ggcgactcga cggcctgatc atcgccacgg cgaccgagcg ggcgcccggg
     2941 ctcgccgagc gaatcggctc gttccagagc gctgtcctgt tggatcgcga ggtgccgggc
     3001 gctcgcgccg acgcagtgct ctccgatcac ggaactggcc tcgccgacgc catccggcac
     3061 ctggcaaggc tggggcatcg acgcattgcc ctcatcgcgg gcaccgccga gcagcgtggc
     3121 ggtcgcgtac gcatcgaccg ctaccacgag gagctcgacg cgctggggct cgagcacggc
     3181 aacggcctct gccgcaccgg cgagttgacg gccgaggacg ggtaccgggc gctacgagag
     3241 gtgctgacgc tcgccgaccc cccgacggcg atcatcgccg gcaacaacca ggtgttcgcc
     3301 gggctcttcg cggcgattcg cgacctcggc gttcgcgtcc cgaccgacct ctccgtgatc
     3361 gcgtgcgagg agacggagct caccgccctg cacaatccgc cgctcgacgt ggtcaggcgc
     3421 gacctcgccg agctcgggcg cgaggccgct tcgttgctgt tgacgcggct agaggcaccc
     3481 aggcggaggc gtcgcgtcgt cgtcctcccg agcgccttcg aggcgcgcgg gagctcggct
     3541 ccgccaccga cgcgctgacg gacgcagccg gagggctagc cgccgcctac cgcccgctcg
     3601 cagcgcgccg cgaaggcgtc gatctccgcg gcgccgtcga gcgcaccgag ctcgaggtcg
     3661 tagcgtcggc tctcccccgc ctccagctcg atcagctcgc cccgctcccg ggcgtcgagg
     3721 cggccgccgg tccgattggt ggaaggctcg atgccgacca cgtaggtccc ctcgccgagc
     3781 atccgccaga cgaagtggtg cgggagctga tcctgtctga acacttcgta tgcgccgaca
     3841 ccgagctcgc ggttgactac ggccgcaggg acggtgcccc tctgctcggg cgcgagctcg
     3901 tgctcgaaca cctgctcgac atagccggcc tccggggcgg cgagcgtccg gtagccctcg
     3961 acgggatggt cgccccgcgc ctccacccca ctcgcggcga cgaggatctc ggagccctcg
     4021 tcgaccacag ggaagccggt gttgacgtgg tacaggagca tgtgcggcgt ccggtcgtgg
     4081 ccgacgttct cgacgacgtc gtgcagcgcc agacgcgact ccccgacgcg cgcctcgatc
     4141 cggcgtcgca gcatcagttg ctcgcccatg acagtcgcct gcagcgtctc accctcggcc
     4201 cagaggacgc actcgtcgcc ttcccaggcc tcgccgtagc cggcgagccg agccgggcgg
     4261 ttcgagaccc gaccgtgcag gccgaacgtc tcggtctgct tcggcgggta gtggtactgc
     4321 gcggcggtgt cctctgccat gaacagcgcg tgctcgatcc cgcaggttgt cagcaggccg
     4381 ccgaggaagc cgcggaagaa gccgagaccc tcgggctcgt agaaccacgg cccctggacg
     4441 cccaccgccg aggtccaggc aagcggggtg ccgcggagct cacagcggcc gatgtcgaag
     4501 gcccggtcga cgaggacgtc gaacgtgaag ccggtgccgg tcctgaactc cagcacacgc
     4561 acgccccgac caccgccgtc acccagcgtg acgaggcgga ttcccgcgac ctgatcgagg
     4621 ctgccgacgc ggcgcgatag gtcgcggcgc gcgtagcgtc gtccgaagag ctcaggcatg
     4681 ccgacggagc ctaacgccgg cgtccgtccg gttcaggccg gaggcgactc gtatcgtcgt
     4741 gatgccgaat gggccgctcc atacgtcgag tagccgccgt cgaccgagac ggcctgcccg
     4801 gtcacgaaac ccgctcccga gatgcaaagc atcaccacgg ccccggcgac gtcctccggc
     4861 agggcgaggc ggcccgccgg cgtgcggtcg atgatctcgc tctcgtcgag aatcccgctc
     4921 tcgatcgcct cgagcaccat cggcgtgcgc acggggcccg gcaggacggc gttgacgcgg
     4981 acgcctctgt cggcccactc gaccgcgagc gtccgtgtca gcccgacgac gccccctttg
     5041 ctcgccgcgt acggcgcccg ccctggcatc ccgacgactg cgccggtgat cgagccgatg
     5101 ttgacgat
//
LOCUS       DBNGNK010000138         2673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_166697,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000138 DBNGNK010000000
VERSION     DBNGNK010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_166697"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..774
                     /locus_tag="AC150V_04015"
     CDS             22..774
                     /locus_tag="AC150V_04015"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3762119.1"
                     /translation="MTITPRQAFTVEPIRTQRTFEAAIDRLTDGLERSGLHRGDRLPS
                     EDELARMLEISRPTLRQALRVLELSGVLAVRRGKGGGIFVASELLPTVAISSAVAVEE
                     GVAAETLRARRVLETAVTREAVSRATAEDYARLERSVELMRSYLGERPLVMRADAMFH
                     RALVRSCHNRVLESAMREVAKALAPIRDAYSGGVDRDRRTLEVHERQLEAMRSGDWAE
                     LDVVLDEHFRMLEQGFAEAIGLSCEELFPVDS"
     gene            complement(943..1818)
                     /locus_tag="AC150V_04020"
     CDS             complement(943..1818)
                     /locus_tag="AC150V_04020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3762120.1"
                     /translation="MARLLIVNPFASGVDERLVAQVQAVLPADTETALTSAAAEATEL
                     TRLAAGRVEAVYVFGGDGTFNEALNGAERETPIGFIPGGGTSVLPRALGLPRDPVAAA
                     RQVSSGEIRRIGLGRANGRRFGFAAGIGFDAELVRRVDERGRDHVGTRPGDLTFAWTA
                     LRLVGERRGRWRPVLELEGLGRAAFTLVANCSPYTYAGPKGLDFVPEASFDLGLDVVA
                     PTQVRARSLPRLAWYALRGRAPAHAADILYGHDLDRIVVRCDEPLPMQLDGEDVGDVT
                     EVLFEAERDAVAVLV"
     gene            complement(1962..>2673)
                     /locus_tag="AC150V_04025"
     CDS             complement(1962..>2673)
                     /locus_tag="AC150V_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3762121.1"
                     /translation="ADVLHAPDTVGRALALALREGRGGVTLFPLLETGTMAERVVQPA
                     AAVLIRSFVAPGPLVRSARTPIAIAAGGFILVERSFYERAGGHEAIRGEFVDDARLAE
                     RVKAAGGLLVPADSSGRVRVRMYEGAREVWRGWRKNTSIGLAGGSLPVAVAQAASGAV
                     LTVAPALALVRGPRGVGLAGLCAQMLARADVEPIVPTGRRWWPAFPVGALILALLSLV
                     SSVDRVRGRLTWRGRTYE"
BASE COUNT          401 a          956 c          987 g          329 t
ORIGIN      
        1 tcaaggcgta gagttgaact cgtgacgatc accccgcggc aggcgttcac cgtcgagccg
       61 atccggacac agcggacgtt cgaggccgcg atcgaccgtc tgacggatgg gctcgagcgg
      121 tcggggctcc accggggcga ccgcctgccg agcgaggacg agttggcgcg catgctggag
      181 atctcccgcc cgacgctccg acaggctctc cgggtgctcg agctgtccgg tgtgctcgcc
      241 gtccggcgcg gcaagggcgg cggcatcttc gtcgcctccg agctcctgcc gacggtcgcg
      301 atctcgagcg ccgtggcggt cgaagagggg gtcgccgccg agacgctgcg ggcccgccgc
      361 gtcctcgaga cggctgttac gcgcgaggcc gtcagtcgcg cgaccgccga ggactatgcc
      421 cggctggagc gctcggtgga gctgatgcgg agctacctcg gtgagcgccc gctcgtgatg
      481 cgcgcggacg cgatgttcca ccgcgcactc gtgcgctcct gccacaacag ggttctcgag
      541 tcggccatgc gcgaggtcgc caaggcgttg gcgccgatcc gcgatgcgta cagcggcggc
      601 gtcgaccgcg accggcgcac gctcgaggtg cacgagcggc agctcgaggc gatgcggtcg
      661 ggcgactggg ccgagctgga cgtcgttctc gacgagcact tcaggatgct cgagcagggt
      721 ttcgccgaag cgatcgggct gagctgcgag gagctcttcc cggtcgacag ctgacgcgtc
      781 ggcgagccgc cgccctcgcc cggcggccgg gggtcgcagc gtctgggcgg ggactgtccc
      841 ccgacatgcc gcgaagcggc acgtccgagt agtgacgaaa gcgtgacgct ttgtccacgg
      901 aggggacggc gggtctggac gcgttcgaaa gcggcggtgg ggctagacga gcacggcgac
      961 ggcatcccgc tcggcctcga acaggacttc cgtgacgtcg ccgacgtcct cgccgtcgag
     1021 ctgcatcggg aggggctcgt cgcagcgaac gacgatgcgg tcgaggtcgt gcccgtaaag
     1081 gatgtcggcc gcatgcgccg gggcgcggcc cctcagcgcg taccaggcga gccgcggcag
     1141 cgaccgagcg cgcacctgcg tcggggcgac gacatcgaga cccaggtcga agctcgcctc
     1201 cggcacgaaa tcgaggccct tggggccggc ataggtatac ggcgagcagt tcgcgacgag
     1261 cgtgaaggcc gcccgcccga ggccctccag ctcgagcacc ggccgccagc gcccacggcg
     1321 ctcaccgacc aggcgcagcg cagtccacgc gaacgtcagg tctccgggac gcgttccgac
     1381 gtggtcgcgg ccgcgctcgt cgacgcgccg gacgagctcc gcgtcgaagc cgatcccggc
     1441 ggcgaagccg aagcgtcgac cgttcgcccg gccgaggccg atgcggcgga tctccccgga
     1501 cgagacctgc ctcgccgcgg cgacggggtc gcgcggcaaa cccagggcgc gggggaggac
     1561 gctcgtgccc ccacccggga tgaagccgat cggtgtctct cgctcggccc cgttgagcgc
     1621 ctcgttgaag gttccgtcgc cgccgaagac gtagaccgcc tcgacccggc ccgcggcgag
     1681 gcgcgtcagc tccgtcgcct cggcggccgc ggaggtcagg gcggtctcgg tgtcggccgg
     1741 gagcaccgcc tgcacctgcg ccacgaggcg ttcgtcgacg ccactcgcga atgggttgac
     1801 gatcagcagg cgcgccatgt ggagatcgtt gctacagggt gtgcggcgac gggagacgcg
     1861 aagccgcctg cagcctgccc gccaccgtct gtgggtcgat cgcactcact cgggagatgg
     1921 gtagcgtcgc acacgacgat ccacagcgca gaccgcttcg cctactcgta cgtgcgcccc
     1981 cgccaggtca ggcgccctcg cactcgatcg accgacgaga cgagagagag gagagcgagg
     2041 atcagggcgc cgaccggaaa cgcgggccac cagcgacgcc cagtcggcac gatcggctcc
     2101 acatctgcac gggcgagcat ctgggcgcag agccctgcca gcccgacgcc gcgcggcccg
     2161 cgaacgaggg cgagcgcggg cgccacggtc agcaccgctc cggacgccgc ttgggccacc
     2221 gcgacgggaa gggagccgcc tgcgagtccg atcgaggtgt tcttccgcca cccgcgccag
     2281 acctcgcgcg cgccctcgta catgcgaacg cgcacgcggc ccgacgagtc tgcaggaacg
     2341 agcaggccgc ccgccgcctt gacgcgctcg gcgaggcggg cgtcgtccac gaactcgccg
     2401 cgaatcgcct cgtggccgcc ggcccgctcg tagaacgacc gctcgacgag gatgaagccg
     2461 cccgcggcga tcgcgatcgg cgttcgggcc gagcgcacga gcggcccggg agcgacgaag
     2521 gagcggatca ggaccgcggc ggccggctgc accacccgct cggccatcgt gcccgtctcc
     2581 agcagcggga agagcgtgac gccgccgcgg ccctcccgca gcgcgagcgc aagcgcccgg
     2641 ccaaccgtgt ccggcgcgtg gagcacgtcg gcg
//
LOCUS       DBNGNK010000139         4385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1098678,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000139 DBNGNK010000000
VERSION     DBNGNK010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1098678"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="AC150V_04030"
     CDS             <1..1060
                     /locus_tag="AC150V_04030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3762122.1"
                     /translation="KVVSSEPVRGVLRSGHHERLLVVDKENGGKADALNAGLNYSRYR
                     YVCGVDADTIFARDALLRGMRLVMSDPARVIGVTSHLTIALDPERTMAEPPGRRQIER
                     RPFVAFQHLDYLRAFFNNRLAWSRFGFMLCAVGAFQIWRRDVLEEVGGYARGFTCEDI
                     ELTFRVHERYLRRGRDYRILCLPDNVGTTEGPDSIRKLVAQRERWQRVIDESVWHYRR
                     MIFNPRYGSVGVVGTPFYLMSEVLAPVFEALALVSLPLALALGVFDWLAFFLGLVAIA
                     FATGALSALAILSDDLHSRVYQPRSLARLLLLAPLDLFLYRPILVWARLKGTWRFLRG
                     DKGWYKFQRNERAAPRRA"
     gene            complement(1071..1541)
                     /locus_tag="AC150V_04035"
     CDS             complement(1071..1541)
                     /locus_tag="AC150V_04035"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR3762123.1"
                     /translation="MRTPDDLEGRPAPDFTLKADDGREITLSDLRGSAVVLYFYPKDD
                     TAGCTTQACGIRDAWGEFERTGAIVLGVSPDDQESHERFKAKYGLPFRLLADTERTAS
                     EAYGVWRERTRDGKTFMGVERSTFVIDAEGNLAKVMRAVDPETHADEVLAALAG"
     gene            1697..2053
                     /locus_tag="AC150V_04040"
     CDS             1697..2053
                     /locus_tag="AC150V_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762124.1"
                     /translation="MLSAHRLVGVALIGVCVAAALVAGILYWRARGAGPITTHMLALA
                     QTLLVAQVGIGLLLLSDGRRAADQLHYLYGTLALGAALAPWFYAPSEGPRRLLWVAGM
                     TGLAAALGARAYMTGT"
     gene            2050..2418
                     /locus_tag="AC150V_04045"
     CDS             2050..2418
                     /locus_tag="AC150V_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762125.1"
                     /translation="MRRFLSNPTVRGLALVALVAFAIVLFSLEESLATIGGLMRIAFF
                     LAIAFFLFLVWRERRSDIESWSDRARWVFYGAIGLAVLDIGMLIGLSPSGLELIAFVV
                     VLVACAWAIFRIWRDEHTYG"
     gene            2488..3957
                     /locus_tag="AC150V_04050"
     CDS             2488..3957
                     /locus_tag="AC150V_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR3762126.1"
                     /translation="MRRLVRPALGALLVGALLSLAPAASSRSAPDEPWLAYGRTPAVS
                     NAAASPVAPEAARGFQQRWSAKLDGFLTAQPLYVPDVRTLSGNRSIVVVATGGNTVFA
                     LDGSNGEIVWRRKLGPTFPQVCGGRGGIASTPAVDPVRGLIYVMSAQGTLRAIALGSG
                     KPRAGWSVLVVKRVDVETVWSGLRISGRMLYVPVASFCDKRDATGKPWDGRLVAVDRL
                     ARRVTKVFDVVPGANNGGSIWGPGGVAIDPQDGSLWTATANAVVYRKQLVQERAPLAE
                     RVLRLSPTLAVRSQVVQPDDNPTFAGDQGFGGTPTLFQPSGCPPLLGINAKSAYTYVW
                     RRDALGRPPLVRERLGQVANPDEPFFGQPTWVAATRTLVVAGASFEVDGIRRRGAVGL
                     RVGAGCTFSRAWQTEIGGGVQPQPLAAGSVAFVSSTQAGELVALDTGSGEILTRLDTG
                     GAAYTAPMVAGALVVVGSSDGTVRAFGPPEHAAPEGTRR"
     gene            complement(3954..>4385)
                     /locus_tag="AC150V_04055"
     CDS             complement(3954..>4385)
                     /locus_tag="AC150V_04055"
                     /inference="COORDINATES: protein motif:HMM:NF018484.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HIR3762127.1"
                     /translation="ETPYSFLATTKPSVYTDKKRVRQGRVIPRDAPYLVVYPFVKRRP
                     WYFLEFEERGAAMREHAEVGGKFVTVTNHTTYSFGIDDQEFMPAFECEEPSDFMHLMM
                     TLRETEASRWTERDTPIFVGKLTEIRTALDALDGASTRVEA"
BASE COUNT          586 a         1527 c         1495 g          777 t
ORIGIN      
        1 caaggtggtc tccagcgagc cggtgcgggg agtgctgcgg agcggacatc acgaacggct
       61 cctggtggtg gacaaggaga acggcggcaa agctgacgcg ctcaacgctg ggctcaacta
      121 ctcccgctac cgctacgtct gcggcgtgga cgccgacacg atctttgccc gcgacgcgct
      181 cttgcgcggc atgcggctgg tgatgagcga tcccgcccgt gtcatcggag tgaccagcca
      241 cttgacgatt gccctcgatc cggagcggac gatggccgag ccgccggggc ggcgtcagat
      301 cgaacgccgg ccgttcgtcg cgttccagca cctcgactac ctgcgggcgt tcttcaacaa
      361 ccgcctcgca tggtcgcgct tcggtttcat gctctgcgcg gtcggggcgt tccagatctg
      421 gcggcgcgac gtgctcgagg aggtgggcgg gtatgcccgt ggcttcacgt gcgaggacat
      481 cgagctcacg tttcgcgtcc acgagcggta cctccggcgt gggcgcgact accggatcct
      541 ctgcctgccg gacaacgtgg gcacgacgga ggggcccgat tcgatacgca agctcgtcgc
      601 gcaacgtgag cggtggcagc gggtgatcga cgagtccgtc tggcactacc ggcggatgat
      661 cttcaacccc cgctacggat ccgtcggggt cgtggggacg cccttctatc tcatgtccga
      721 ggttctcgct cctgtcttcg aggcgttggc cctcgtctcg ctgccgcttg cgctcgcgct
      781 cggagtgttc gattggctgg ctttctttct cggcctcgtg gcgatcgcgt tcgcgaccgg
      841 cgcgctatcg gcgctcgcaa ttctctccga cgacctccac tcgcgggtct atcagccgcg
      901 ctcgctcgcc agattgttgc tcctcgctcc actcgacctc ttcctgtatc ggccgattct
      961 cgtctgggcg cgcctgaagg gaacctggcg cttcctgcgc ggcgacaagg gctggtacaa
     1021 gttccagcgg aacgagcgcg cggcgccgcg tcgcgcctga gtgcgccgcc tcagccggcg
     1081 agagccgcga gcacttcgtc ggcgtgcgtc tcaggatcga cggcgcgcat gaccttcgcg
     1141 aggttcccct ccgcgtcgat gacgaacgtc gagcgctcga cacccatgaa cgtcttgccg
     1201 tctcgcgttc gctcgcgcca gacgccgtag gcctcgcttg ccgtgcgctc cgtatccgcg
     1261 agtaggcgga acgggaggcc gtacttcgcc ttgaagcgct cgtgcgactc ctggtcgtcc
     1321 gggctcacgc cgaggacgat cgcgccggtt cgctcaaact ccccccaggc gtcccggatg
     1381 ccgcaggcct gcgtcgtgca gcccgccgtg tcgtctttcg gatagaagta gagaacgacg
     1441 gcggagcccc gaagatccga cagggtgatc tcgcggccgt cgtcagcctt gagcgtgaag
     1501 tcgggggcgg gccggccttc caggtcgtct ggtgtccgca tgatgcgaat ctcccgcgtc
     1561 ggtgattgtg tgagagaccg cctggcaggg cggcctggcc agacactagt cgcgagccgc
     1621 atcaatgacg ccggcagacc tgaccgacac ccgctgcgtc gctgcctgac cgcggcccgc
     1681 cggtaaggtc ggcccgatgc tctcggccca ccgtctcgtc ggggtcgcgc ttatcggcgt
     1741 atgcgtcgcg gccgcgctcg tcgccgggat cctctactgg cgcgcgcgcg gggcgggccc
     1801 gatcacgact cacatgctcg cccttgctca gaccctcctc gttgcgcagg tcggcatcgg
     1861 actactgctc ctgtccgacg ggcgccgggc ggccgaccag ctccactacc tctacggcac
     1921 cctcgcgctg ggtgcggccc ttgcgccctg gttctacgcg ccgtccgaag gccctcggcg
     1981 cctgctctgg gtcgccggca tgacgggtct tgccgccgca ctcggtgccc gtgcttacat
     2041 gaccggcacg tgaggcgctt cctctccaat cccacggttc gcgggctcgc actggtagcg
     2101 cttgtggcat tcgccatcgt gctcttctcg ctcgaggagt cgctggcgac gatcggcggg
     2161 ctcatgcgga tcgccttctt cctggcgatc gcgttcttcc tgtttctggt ctggcgcgag
     2221 cgccgcagcg acatcgagtc gtggtcggac cgtgcgcgct gggtcttcta cggtgccatc
     2281 ggactcgccg tgctcgacat cggcatgctg atcggactct cgccgagcgg cctcgagctg
     2341 atcgccttcg tcgtcgttct cgtcgcgtgc gcctgggcga tcttccgcat ctggcgcgac
     2401 gagcacacgt acggctgagt cgcgcccgcg cccacgccgt ctattctcgc gcctccaccg
     2461 ccccgagacg agcatgggat gacctttatg agacgccttg tccgcccggc cctgggagcg
     2521 ctcttggtcg gcgcgctcct ctccctggcg cctgcggcga gctcccgcag cgcccccgat
     2581 gagccctggt tggcatacgg ccgcacgccc gcggtctcca acgctgcggc gagccccgtc
     2641 gccccggagg cggcccgcgg gtttcagcag cggtggagcg cgaagctcga cggcttcttg
     2701 accgcgcagc ccctctacgt cccggacgtc cgcaccctca gcggcaaccg ctcgatcgtc
     2761 gtcgtcgcga ccggcggcaa cacggtgttc gccctcgacg gctcgaacgg cgagatcgtc
     2821 tggcggcgca agctcgggcc gacgtttcca caggtctgcg gcggcagggg cggtatcgca
     2881 tcgactcctg ccgtcgaccc ggtacgcggc ctgatctacg tgatgtcggc acagggcacc
     2941 ttgcgagcca tcgcgctcgg cagcggcaag ccccgcgcag gctggagcgt cctggtggtg
     3001 aagcgcgtcg acgtcgagac cgtctggagc gggctgcgga tctcgggtcg gatgctctac
     3061 gtccccgtcg cttcgttctg cgacaagagg gacgcgaccg gaaagccctg ggacgggcgc
     3121 ctcgtagccg tcgaccggct cgcccggcgg gtgacgaagg tgttcgacgt cgtgcccggc
     3181 gccaacaacg gaggctcgat ttgggggccg ggcggagtcg ccatcgaccc gcaggacggc
     3241 agcctctgga cggcgacggc gaacgccgtc gtctaccgaa agcagctggt gcaggagagg
     3301 gcgccgctcg ccgagcgggt tctccgcctt tcaccgacgc ttgcggtgcg ctcccaggtc
     3361 gtccagcccg acgacaatcc gacattcgcc ggagatcagg gcttcggggg aacgccgacg
     3421 ctgttccagc cttccggctg cccgccgctt ctcggtatca acgccaagag cgcctacacg
     3481 tacgtctggc ggcgcgacgc gctcggtcgt cccccgctcg tgcgagaacg cctggggcag
     3541 gtggcgaatc ccgacgagcc gttcttcggc caacccacct gggtggccgc cacgcggacg
     3601 ctcgtcgtgg ccggtgcctc cttcgaggtc gacggaatcc ggcgccgcgg tgccgtcggg
     3661 ctgcgtgtcg gtgccggctg caccttctcg cgcgcctggc agaccgagat cggcggagga
     3721 gtgcagccgc agccgctggc ggcgggcagc gtcgccttcg tctcgtcgac ccaggccgga
     3781 gagctcgtcg cgctggatac gggcagcggc gagatcctga cgcgtctcga taccggcggt
     3841 gcggcgtaca ccgcccccat ggtcgccgga gcactggtcg tcgtgggcag ctccgacgga
     3901 acggtgcggg cgttcggccc gccagagcac gcggcgccgg agggaacgag gcgctaggcc
     3961 tcgactcggg tggaggcgcc gtcgagcgcg tcgagcgccg tccggatctc ggtgagcttg
     4021 ccgacgaaga tgggcgtgtc gcgctcggtc cagcgcgaag cttccgtctc gcggagcgtc
     4081 atcatcaggt gcatgaaatc cgacggctcc tcgcattcga aggccggcat gaactcttgg
     4141 tcgtcgatcc cgaaggagta ggtcgtgtgg ttcgtgaccg tgacgaactt gccgccgacc
     4201 tcggcgtgct cgcgcattgc cgccccgcgc tcctcgaact cgaggaagta ccacgggcgc
     4261 cgcttgacga acggatagac cacgagatag ggggcgtcgc gcgggatcac gcgaccctgg
     4321 cgaacacgct tcttgtcggt gtagacggac ggcttcgtcg tcgcgaggaa cgagtacggc
     4381 gtctc
//
LOCUS       DBNGNK010000140         3608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1456122,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000140 DBNGNK010000000
VERSION     DBNGNK010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1456122"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="AC150V_04060"
     CDS             <1..214
                     /locus_tag="AC150V_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762128.1"
                     /translation="GVVGHGYGPLRHHPPVCARVPGMSSDLHRIIDFRSSLVFGFCCL
                     YPWAHAAIVVDGIPRGYPVRVLRSAA"
     gene            315..>3608
                     /gene="gltB"
                     /locus_tag="AC150V_04065"
     CDS             315..>3608
                     /gene="gltB"
                     /locus_tag="AC150V_04065"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR3762129.1"
                     /translation="MSATVHDTTRERDACGIGLVADVRGRTSRELLDRALAGLAAVGH
                     RGAWAADGLTGDGAGLLLPLSPPLTGGEPGAGLAMVFLRDERGRAMLEEACRAEGLEP
                     AGWRDVPIERGALGEYAQQTMPDVAQLLIAPSDEPDAEERAYRARRRAELSGFLYIAS
                     LSFRTVTYKALCAANQLAHAYPDLLNPELVGAFAIFHQRFSTNTEPTWERAQPFRLLC
                     HNGEINTIEGNVAWMEARAHARGLDSSLGPALDRAGSDSALLDNALELLVRNGKDLSE
                     AASMLIPPAWQNDPRIPPAVRDFHRYHALLLEPWDGPAGIVFTDGRACGAALDRNGLR
                     PLRIAITDDGLVACASEAGAVPLPEGVGVRRGRLGPGQALSVDPERGLRLDGEVKRDF
                     ARRQPYGEWVEASIDRRGVGEPPSAPEDDLTRRHILHGYTREELSLMLRPIAQSGHDP
                     VYSMGDDAAISPLAGRARPLASYFRQRFAQVTNPAIDHLRERTMMSVATLLGPRGRIV
                     AEGPLEPLVVLPSFLVYPSGLDDLEPVRLDATFTEAEGLSGALDRLTRLAAEAVAGGS
                     TCICISDEAAGADRAAIPALLALSSTHIRLVEEGLRTRCSLLVSTDEARDSHMIATLV
                     GYGAGAICPRLALETVAQLAAADKVGGDRPSPEEAQGRLLAAFEDGVLKVMSKMGISD
                     IASYRGARLFDAVGLDRELCDRWFGGTVSRIGGVGLERLEAEAIARLAASNIESGKLE
                     NPGFYKYRKGGEPHATTPEVVEALKESVSAAHALRTATKEGRSDLYDRFASLVNERDP
                     IEPRDLLELVPAAAPVELDEVEPATEIVRRFSSGAMSHGALSLEAHETIAIALNRLGA
                     RSNCGEGGEDPARYRTERNSAIKQIASARFGVTAEYAVHADELQIKIAQGSKPGEGGQ
                     IPAHKVSEEIARLRNTQPGVSLISPPPHHDIYSIEDLAQLIFDLREVNPEADISVKLV
                     AEAGVGLVAAGVAKAHADIIHVGGADGGTGASPLPSIKHAGVPWELGLAETQQALVAS
                     GLRGRVRLRVDGGFKTGRDVVMAALLGADEFSFGTSLLLAEGCLMVRSCHLDSCPVGI
                     ASQKPELREK"
BASE COUNT          518 a         1240 c         1275 g          575 t
ORIGIN      
        1 tggcgttgtc ggacacggat acggcccgct tcggcatcac ccgccagtgt gcgccagggt
       61 tccggggatg tcaagcgatc ttcaccgaat catcgatttt cgatcgtccc ttgtgttcgg
      121 gttctgctgt ctctaccctt gggcgcatgc tgcgatcgtc gtggacggca ttccgcgcgg
      181 gtaccccgtt cgcgtccttc gcagcgccgc ctagcggcta gccggcaccg cagcctctcc
      241 cgcccgccgg aatagctcac aacacaccgg cgggagaatc gagaccccta ccccgagagg
      301 agcgaagagg cgcggtgagc gcgacggttc acgacacaac ccgcgaacgc gatgcgtgcg
      361 gcatcgggtt ggtcgccgac gttcgcggcc gtacctcccg cgagttgctc gaccgggctc
      421 tggcgggcct cgccgcggtc ggccatcgag gcgcctgggc tgccgacggg ctgacgggtg
      481 acggtgccgg cctcctgctc ccgctctcgc ctccgctcac gggcggcgag cccggcgccg
      541 ggcttgcgat ggtcttcctg cgcgacgagc ggggccgggc gatgctcgag gaggcctgtc
      601 gggcggaggg gctcgagcct gccgggtggc gagacgttcc gatcgagcgc ggcgcgctcg
      661 gtgagtacgc gcaacagacg atgccggatg tcgcgcagct gctgatcgct ccctccgacg
      721 agccggacgc cgaggagcgc gcttaccggg cgcggcgtcg ggcagagctc tcgggcttcc
      781 tgtacatcgc ctcgctctcg ttccggaccg tcacgtacaa ggctctgtgc gccgccaacc
      841 agctcgcgca cgcctatccg gacctcctca acccggagct ggtcggggcc tttgcgatct
      901 tccatcagcg cttctccacg aacacggagc cgacgtggga gcgtgcgcag ccgttccgcc
      961 ttctctgcca caacggcgag atcaacacca tcgaaggcaa cgtcgcttgg atggaggcgc
     1021 gggcccatgc ccgtggcctg gacagttctc tcgggccggc gctcgatcgg gccgggagcg
     1081 actcggcgct gctcgacaat gccctcgagc tgctcgtccg gaacggcaag gatttgtccg
     1141 aggctgcctc gatgctgatt ccgccggcct ggcagaacga tccgcgcatt ccgcccgctg
     1201 tgcgcgactt ccaccgctac cacgcgctgc tgctcgagcc ctgggacggt ccggccggaa
     1261 tcgtgttcac cgacggtcgc gcctgcgggg ccgcgctcga ccggaacggg ctgcggccgc
     1321 tccgcatcgc gatcacggac gacggcctcg tcgcctgcgc gtcggaggcc ggcgcggttc
     1381 ctctgccgga gggcgtgggc gtccggcgtg gtcgcctcgg tcccggccag gcgctctcgg
     1441 tcgaccccga gcgcggtctg cgcctggacg gggaggtgaa gcgcgacttc gctcgtcgcc
     1501 agccgtacgg cgagtgggtc gaggcgtcga tcgaccggcg cggggtgggt gagccgccct
     1561 cggctccgga ggacgacctg acccggcggc acatcctgca cggctacacc cgcgaagagc
     1621 tgagcttgat gcttcggccg atcgcgcagt cggggcacga ccccgtctac tcgatgggtg
     1681 acgacgcggc gatctcacca ctcgccgggc gggcgcggcc gctcgcctcg tattttcggc
     1741 agcgcttcgc gcaggtcacc aacccggcca tcgatcacct ccgggagcgg acgatgatgt
     1801 cggtcgccac gctcctcggc ccccgcggtc gcatcgtcgc ggaggggcca ctcgagcctt
     1861 tggtcgtcct gccgagcttc ctggtctatc cgtccggact tgacgacctc gagccggtca
     1921 ggctcgacgc gaccttcacc gaggcggagg gcctctcggg cgcgctcgat cgcttgacga
     1981 gattggcggc agaggcggtc gccggtggat cgacctgcat ctgcatctcg gacgaggcgg
     2041 ccggcgccga ccgggctgcg attcctgcct tgcttgcgct gtcgtccacg catatccggc
     2101 tcgtcgagga ggggctgaga acgcggtgct cgctgctcgt gtccaccgac gaggcgaggg
     2161 actcgcacat gatcgcgacg cttgtcggct acggggcagg cgcgatctgt ccgcggctcg
     2221 cgctcgagac cgtcgcgcaa ctcgccgccg ccgacaaggt cggcggcgac cgtccgagcc
     2281 cggaggaagc acagggccgt ctgctcgcgg cgttcgagga cggtgtgttg aaggtgatgt
     2341 cgaagatggg catctcggac atcgcgagct accgcggtgc gcgcctgttc gacgccgtag
     2401 ggctcgaccg cgagctctgt gaccggtggt tcggcggaac ggtttctcgg atcggcggcg
     2461 tcgggctcga acgcctcgag gctgaggcca tcgcgcggct cgccgcgtcg aacatcgagt
     2521 ccggcaagct cgaaaacccg ggcttctaca agtaccggaa gggcggcgag ccgcacgcga
     2581 cgacacccga ggtcgtcgaa gcgctgaagg agagtgtctc ggccgcgcat gccttgcgca
     2641 cggcgacgaa ggagggccgc tccgacctct acgaccggtt cgccagcctc gtcaacgagc
     2701 gcgacccaat cgagccgcgc gatctcctcg agctggttcc ggcggcagcg ccggtcgagc
     2761 tcgacgaggt ggagccggcg acggagatcg tgaggcgctt ctcgagcggc gccatgtcgc
     2821 acggagcgct ctcgctcgag gcacacgaga cgatcgccat cgcgctgaac cgtctcggcg
     2881 cgcgctcgaa ctgcggcgag ggcggcgagg atcccgctcg ctatcgaacc gagcgcaact
     2941 cggccatcaa gcagatcgcg tcggcgcggt tcggcgtcac cgccgagtac gccgtccatg
     3001 ccgatgagct gcagatcaag atcgcgcaag gctcgaagcc cggcgagggc gggcagattc
     3061 cggcgcacaa ggtgtccgag gagatcgcac ggctgcgcaa cacgcagcca ggcgtctcgt
     3121 tgatctcgcc cccgccccac cacgacattt actcgatcga agacctggct cagctgatct
     3181 tcgacctacg cgaggtgaac ccggaggcgg acatctcggt gaagctcgtg gcggaggccg
     3241 gtgtgggtct tgtggccgca ggggtcgcca aggcacacgc cgacatcatc cacgtgggcg
     3301 gggcggacgg aggcacgggc gcgagcccgt tgccctcgat caagcacgcg ggcgttccgt
     3361 gggagctggg tctggccgag acgcagcaag ccctcgtcgc gagcggcctc cgcgggcggg
     3421 ttcggctgcg cgtcgacggc ggcttcaaga ccggccgtga cgtcgtcatg gcggcgttgc
     3481 tcggagccga cgagttcagc ttcgggacgt cgttgctcct tgcggaggga tgcctcatgg
     3541 tccgctcctg ccatctcgac agctgcccgg tgggcattgc gagtcagaag cccgagctgc
     3601 gcgagaag
//
LOCUS       DBNGNK010000141         3163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_23954,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000141 DBNGNK010000000
VERSION     DBNGNK010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_23954"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1551
                     /locus_tag="AC150V_04070"
     CDS             118..1551
                     /locus_tag="AC150V_04070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR3762130.1"
                     /translation="MHDAVVVGSGPNGLAAAITLAREGRSVLVLEGRETVGGGLRTEE
                     LTLPGFRHDVCAAIHPLGVSSPFFRSVPLELHGVEWIQPPAPLAHPLDDGGAVLLERS
                     LRDTATGLDGDGGAWTSLVGPFARSAGDLLPDILAPPRPPRHPLLLARFGAKALRSAT
                     GLARSAFGGERARALFAGNAAHSMLPLDAPATASFGLVLALLGHAVGWPFPRGGSQAI
                     ADALVCCLRSLGGEVRTGIPVSRLEGLPPAHAVLLDVTPRQLIALGGDRWPTRYRRAL
                     ERFRYGPGVVKVDYALSEPVPWRAVECRRAATLHLGGTLAEIAAAERDVAQGKQPSRP
                     FVLAAQQSLFDGTRAPAARHTLWAYTHVPNGWRAIDSAVEALEAQLERYAPGFRDVVI
                     MRSVLGPAELEARNPNYVGGDINGGSAELRQLFARPVARPVPYATPIEGVYVCSSSTP
                     PGGGVHGMCGYYAAQAALRRRRLRGWR"
     gene            1542..1934
                     /locus_tag="AC150V_04075"
     CDS             1542..1934
                     /locus_tag="AC150V_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3762131.1"
                     /translation="MALIHYDDATPAVRAVFDDIKQARGVEDVNNFWKALAHDPVTLR
                     RTWESVKEVMAPGALDALTKELVYVAVSVTNGCEYCIASHTAGARAKGMTAEQLAELL
                     AVVGMANETNALATGYGVPVDDSFRRDG"
     gene            1931..2830
                     /locus_tag="AC150V_04080"
     CDS             1931..2830
                     /locus_tag="AC150V_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR3762132.1"
                     /translation="MTIGALGVWLGAFAVAPAEVARPAAAEIERLGYSALWYPEGLGT
                     RESFTNGAVLLGATERIRVCSGITNIWGRDPVAAANAARVLDDGFEGRFLLGLGVSHP
                     RQVDPRGHRYEKPVARMRSFLDGMDDDPFVTPDGTSAARPPVPRVLAALRPPMLRLAA
                     EKAQGAHTFLVPVEHTRRAREILGPDALLVVEQKVLLEPAADAARERGRRALAWYLDT
                     PNYVESLRWLGFTDEDLAGEPSDRLVDALVAWGDLGRIVDRLREHLEAGADQVAVQPL
                     GDDSDPLGLGTLRALSPAVAELG"
     gene            complement(2840..>3163)
                     /locus_tag="AC150V_04085"
     CDS             complement(2840..>3163)
                     /locus_tag="AC150V_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR3762133.1"
                     /translation="DGERIAFMSNPAGYDAPSRDWDEEIYVANVDGTDVTLVSNRRGN
                     DHWPPSWSSDGRCLVWQGDAPGGGMRAEIYAAAVDVPDPQAQAVTETDAFELMPAWTH
                     GDCPL"
BASE COUNT          399 a         1126 c         1140 g          498 t
ORIGIN      
        1 acgtctcctg acgctcgacg ggcgccgcgg cgcccgacgt gtcactaccc gctcgaccac
       61 cggggtcgag cgggcgtatc tcggcccggc ttgaggcccg gcgactagcg tcgaagcgtg
      121 cacgacgctg tcgtcgtcgg ttcggggccg aacgggttgg cggccgcgat cacgcttgcc
      181 cgcgaaggcc gttcggtgct cgtgctcgaa ggtcgcgaga ccgttggcgg tggactgcga
      241 acggaggagc tgaccctgcc gggcttccgg cacgacgtat gcgccgcgat tcacccgctc
      301 ggggtgtcct cgcccttctt ccgctccgtg ccgctcgaac tgcacggtgt cgagtggatc
      361 cagccacccg caccgctggc ccatccgctc gacgacggcg gcgcagtgct gctcgagcgc
      421 tcgctccgcg atacggcgac aggactcgac ggcgacgggg gcgcctggac gagcctcgtc
      481 gggccgttcg cgcgctcggc cggcgatctg cttcccgata tcctcgcgcc gccgcgtcca
      541 ccgcggcacc cgctgctgct cgcacgcttc ggtgcgaagg cacttcgctc ggccaccggg
      601 ctcgcgcggt cggccttcgg gggggagcgg gcccgggcgc tcttcgcagg caatgcagcg
      661 cactcgatgc ttccactcga cgcccctgca acggcctcgt tcggactcgt tctggcgctg
      721 ctcggacacg ctgtcggctg gccgttccct cgcggcggct cgcaggcgat cgccgacgcg
      781 ctcgtctgct gtctgcgctc gctcggcggc gaggtgcgaa cggggatacc ggtgtcacgg
      841 ctggaggggc tgccgcccgc gcatgccgtc ctactcgatg tgacgccgag gcagctgatc
      901 gcgctcggcg gggatcgttg gcctacccgc tatcggcggg ccttggagcg cttccgctac
      961 ggccctgggg tggtgaaggt cgattacgcg ctctccgagc ccgttccgtg gcgggccgtc
     1021 gagtgtcggc gggcggcgac cctgcacctc ggcgggacgc tcgcggagat cgccgccgcc
     1081 gaacgggatg tggcgcaggg caagcagccg agccgcccct tcgtcctggc tgcccaacag
     1141 agcctcttcg acgggacgag ggcgccggcc gcgcgacaca cgctctgggc ctacacccac
     1201 gtcccgaacg gctggcgcgc aatcgacagc gccgtcgagg cgctcgaggc gcagctcgag
     1261 cgctacgcgc cgggcttccg ggacgtcgta atcatgcgga gcgtgctcgg gccggctgag
     1321 ctcgaggccc gcaacccgaa ctacgtcggc ggtgacatca acggaggcag cgccgagctg
     1381 cgacagctgt tcgcccgtcc cgtcgcccgt ccggtgccgt atgcgactcc gatcgagggt
     1441 gtctacgtct gttcctcctc gacgccgccg ggcggcgggg tgcacggcat gtgtggctac
     1501 tacgcggcgc aggctgcgct gcggcggcgt aggctccgcg ggtggcgctg atccactacg
     1561 acgacgcgac acccgccgtg cgcgcggtct tcgacgacat caagcaggcc cgcggtgtcg
     1621 aggacgtcaa caacttctgg aaggcgctcg cgcacgatcc ggtcacgctc cgtcgcacgt
     1681 gggagagcgt gaaggaggtc atggccccgg gtgcgctcga cgcgctgacg aaggagctcg
     1741 tctacgtcgc cgtgtccgtc accaacggct gcgagtactg catcgcgagc cacacggccg
     1801 gagcgcgggc gaaggggatg actgccgagc agctcgccga gctcctcgcg gtggtgggga
     1861 tggccaacga gacgaatgcg ctcgccacgg ggtacggggt gccggtcgac gattcctttc
     1921 gccgcgacgg atgactatcg gcgctctcgg agtctggctg ggggccttcg ccgtcgcgcc
     1981 cgccgaggtt gcccgccctg cggcggccga gatcgagcgg ctcggctact ccgccttgtg
     2041 gtatcccgaa ggactcggta cgcgggagtc cttcacgaac ggcgccgtcc tccttggtgc
     2101 gaccgagcgg atccgtgtct gcagcggcat caccaatatc tggggacgcg atcccgtcgc
     2161 cgcggcgaac gccgcgcgcg tgctcgacga cggattcgag ggacggttcc tgctcgggct
     2221 cggcgtcagt cacccgcggc aggtcgaccc acgcggccac cgctacgaga agccggtcgc
     2281 gcgcatgcgt tcctttctcg acgggatgga cgacgacccc ttcgtcaccc cggacggcac
     2341 gtcagcggca cgcccgccgg tgccccgcgt gctcgcggcg ctgcgcccgc cgatgctccg
     2401 gctggcggcg gagaaggcgc agggtgcgca cacgttcctg gttcccgtcg agcacacccg
     2461 tcgcgcgcgt gagatcctcg ggcccgacgc cctgctggtg gtggagcaga aggtcctgct
     2521 cgagccggcc gcggacgccg cgcgggagcg ggggcgccgt gcgctcgcct ggtacctcga
     2581 cacgccgaac tacgtcgaga gccttcgctg gctcggcttc acggacgagg atctggccgg
     2641 tgagccctct gatcggctgg tcgacgcgct cgtcgcgtgg ggtgacctcg ggcgcatcgt
     2701 cgatcggctc cgcgagcatc tcgaggccgg cgccgaccag gtcgcggtgc agccgctcgg
     2761 cgacgactcc gacccgctcg gtctcgggac cctgcgggcg ctgtcgcccg cggtcgcaga
     2821 gctcggctag tccgatcggt cagagcgggc agtcgccgtg cgtccacgcg ggcatgagct
     2881 cgaaggcgtc ggtctcggtc accgcctgcg cctgtggatc aggaacgtcg acggcggcgg
     2941 cgtagatctc cgctctcatg ccgcccccgg gggcgtcgcc ctgccagacg aggcaacgcc
     3001 catcggagct ccagctcggc ggccagtggt cgttgccacg ccggttgctg acgagggtca
     3061 cgtcggttcc gtcgacgttc gcgacgtaga tctcctcgtc ccagtctctg gagggcgcgt
     3121 cgtagccggc cgggttcgac atgaaggcga tgcgctctcc gtc
//
LOCUS       DBNGNK010000142         2810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_954642,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000142 DBNGNK010000000
VERSION     DBNGNK010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_954642"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="AC150V_04090"
     CDS             <1..366
                     /locus_tag="AC150V_04090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR3762134.1"
                     /translation="GGFDCSGFVWRVYKLEPFAKAPFLGEMLQGRSTFSMSAEVTKRA
                     RIDRTRLRPADLVFFGDSGAKSIPAAMGHLGLYVGNGWFVHSSSHGVTLQPMQGWYDE
                     RLAWGRRPLAEAGLTRPAA"
     gene            429..1961
                     /locus_tag="AC150V_04095"
     CDS             429..1961
                     /locus_tag="AC150V_04095"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019586024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3762135.1"
                     /translation="MTEVAAAPSDRADGEITRISHWINGALVEGTSGRSGPVFNPALG
                     VQTGEVDFASAEEVDAAVAAARKAFATWRSVSLARRAELMFRVRELVHARREEIARFL
                     TLEHGKVLSDAMGEVTRGLEVIEYACGIPTLLKGDFSEQASTGIDVYSIRQPLGVVAG
                     ITPFNFPAMVPMWMWAPAIACGNTFVLKPSEKDPSASIYVADLLQEAGLPDGVFNVIH
                     GDKVAVDRLLEHPDVEAVSFVGSTPIARYIYETGTKHGKRVQALGGAKNHMVVLPDAD
                     IDMAADAAVSAAYGSAGERCMAISAVVAVGGVADPLVKAIQERLPQVKVGNGLDADSE
                     MGPLVTRAHRDKVASYLDSGREQGASVLADGRETAPEGDGFFLGVSLIDDVTPEMDCY
                     RDEIFGPVLSIVRVDTYAEALELVNENPYGNGTAIFTRDGGVAREFQFDVNVGMVGVN
                     VPIPVPVAYYSFGGWKSSLFGDRHIYGPEGIDFYTQGKVVTSRWPDPATSEVDLGFPQ
                     TR"
     gene            2080..>2810
                     /locus_tag="AC150V_04100"
     CDS             2080..>2810
                     /locus_tag="AC150V_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR3762136.1"
                     /translation="MPAGRPHDDMDLLRPEDVNAENLPVAMRGYDKHEVERRLAWTKE
                     SYSRVLQQRDALREQLDGVDERISSAEGEVRVSAKELADQAQRAATAEDELAQARERL
                     TSAEAELRKADAERKLALDDMRSAKEEAVRLASRVEALENALRNRAGDASAERTPPAT
                     PAVPTHSPPDAEAAALLVAATRAAEDLRMAARDQAQKAMKKARERADALTGEAEEERR
                     AAEQARERASQQAARTEAARRAADEA"
BASE COUNT          460 a          924 c          996 g          430 t
ORIGIN      
        1 ggtggcttcg actgctcggg cttcgtgtgg cgtgtctaca agctcgagcc cttcgcgaag
       61 gccccgttcc tcggagagat gttgcagggg cgctcgacgt tctcgatgag tgccgaggtg
      121 acgaaacgag cgagaatcga tcgcacgcgg ctccggccag ccgacctcgt cttcttcggc
      181 gacagcggcg cgaagtcgat tcccgccgcg atgggccacc tgggcctcta cgtcggcaac
      241 ggctggttcg tccactcgtc gtcgcacgga gtgacgctgc agccgatgca gggctggtac
      301 gacgagcgcc tggcctgggg ccggcgaccg cttgccgaag ccgggctcac acgcccggcg
      361 gcgtagccgc ctccgtccgg atccgcgata ccctgccggg aacgcgattc ctgcaggagg
      421 gacacgagat gaccgaggta gcagcggcgc cgagcgatcg cgccgacgga gagatcaccc
      481 ggatcagcca ctggatcaac ggcgccctcg tcgaggggac atcgggccgg agtggcccgg
      541 tcttcaaccc ggcgctcggc gttcagaccg gcgaggtcga cttcgcctcg gcagaggagg
      601 tcgacgcggc agtcgccgcg gcgcgcaagg cgttcgcgac ctggcgcagt gtctcgttgg
      661 cccgtcgtgc ggagctgatg tttcgcgtgc gcgagctggt gcacgcccgc cgcgaggaga
      721 tcgcgcggtt cctgacgctc gagcacggca aggtgctctc ggatgcgatg ggtgaggtaa
      781 cgcgcgggct cgaggtgatc gagtatgcgt gcgggatccc gacgctgctg aagggcgact
      841 tctcagagca ggcgtcgacc ggcatcgacg tctactcgat ccgacagccg ctgggagtcg
      901 tggccggcat cacaccgttc aacttccccg caatggttcc gatgtggatg tgggcgccgg
      961 ctatcgcgtg cggtaacacg ttcgtcctca agccgtccga gaaggatccc tccgcctcga
     1021 tctacgtcgc cgacctgctg caggaggcag ggctgccgga cggcgtcttc aacgtcatcc
     1081 acggcgacaa ggtcgccgtc gaccggctcc tggagcaccc tgacgtcgag gcggtcagct
     1141 tcgtcggctc gacgccgatc gcccggtaca tctacgagac cggcacgaag cacggcaagc
     1201 gcgtccaggc gctcggtggc gcgaagaacc acatggtcgt gctgcccgat gccgacatcg
     1261 acatggccgc ggacgcggcc gtcagcgcgg cgtacggctc ggccggagag cgctgtatgg
     1321 cgatctcggc ggtcgtggcg gtcggaggcg tggccgaccc gctcgtgaag gccattcaag
     1381 agcgcctgcc gcaggtcaag gtcggcaatg gcctcgacgc ggattccgag atgggcccac
     1441 tcgtcacgcg ggcgcaccgc gacaaggtgg cctcctacct ggattccggc cgcgagcagg
     1501 gcgcttcggt gctggccgac gggcgcgaga cggctcccga aggtgacggg ttctttctcg
     1561 gcgtctccct gatcgacgac gtcactccgg agatggactg ctatcgggac gagatcttcg
     1621 gtcccgtgct ctccatcgtc cgcgtcgaca cctacgcgga ggcgctggag ctcgtcaacg
     1681 agaacccgta cggcaacggg acggcgatct tcacccgcga cggtggcgtt gcccgcgagt
     1741 tccagttcga cgtcaacgtc ggcatggtcg gggtgaacgt cccgatcccc gtgcccgtcg
     1801 cctactactc gttcggtggc tggaagtcgt cgctcttcgg cgacaggcac atctacgggc
     1861 ccgagggaat cgacttctac acacagggga aggttgtcac cagccgctgg cccgaccccg
     1921 ccacgagcga ggtcgatctc ggcttcccgc agacgcgctg agcgaatcgc cggggcggtt
     1981 cttcggggcg ccccgttttc cgctccaagc tggaatttcg gcgttcagtc ttaacgccgt
     2041 tcccttccaa atcggcgccc gaccgagtac cttcattacg tgccagcggg gaggccacac
     2101 gacgacatgg acctgctgcg tccggaggat gtcaatgccg agaacctccc tgtcgccatg
     2161 cgggggtacg acaagcacga ggtcgagcgt cggctcgcgt ggacgaagga gagctattcg
     2221 cgcgtcctgc aacagcggga cgccctgcgt gagcagctcg acggtgtcga cgagcggatc
     2281 tcgtcggccg agggtgaggt gcgggtcagc gccaaggagc tcgccgatca ggcgcaaagg
     2341 gccgcgacgg cagaggacga gctcgcccaa gctcgcgagc gtctaaccag cgcagaggcg
     2401 gagcttcgca aggccgatgc cgagcgcaag ctggctctgg acgacatgcg cagcgccaag
     2461 gaggaagccg tccgtttggc cagccgggtg gaggcgctcg agaacgctct tcggaaccgc
     2521 gccggcgacg cttccgccga gcgcacgccg ccggcgacgc cggcggtccc gacgcactcg
     2581 cccccggacg cagaggcagc ggcgctgctc gtcgctgcta cgcgggctgc tgaagatctt
     2641 cggatggcgg cccgggatca agctcagaaa gcgatgaaga aggctcgtga gcgggccgac
     2701 gcgctcaccg gcgaggcgga ggaggagcgc cgggcagccg agcaggcacg tgagcgagcg
     2761 tcgcagcagg ctgcgcgcac cgaggcggct cgacgagccg ccgacgaggc
//
LOCUS       DBNGNK010000143         3458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1312775,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000143 DBNGNK010000000
VERSION     DBNGNK010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1312775"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="AC150V_04105"
     CDS             complement(<1..589)
                     /locus_tag="AC150V_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015272.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - L-tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HIR3762137.1"
                     /translation="MAGQAEEDFSQPMLPGTAGSDYERYLNTEELLSLQKDAGEWVHR
                     DELLFQVVHQSSELWLKLATADAQAAARHVEAGQIAAALRLLRRPALCLRYLTDQLDM
                     LEQMSPWEYQEIRKVLGHGSGFDSPGFAELRRVMPQLEAAFNAAREDAGLSLAELYVH
                     GRDHEELYQLAESLIELDEWVQAWRVRHYRVVARII"
     gene            612..2006
                     /locus_tag="AC150V_04110"
     CDS             612..2006
                     /locus_tag="AC150V_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR3762138.1"
                     /translation="MDDLFADSAREQQATTAPLPQRIRPRALDELVGQRHVLGEGSAL
                     RTAIEQDRPPSMILHGPPGTGKTTLARIVAERTGAEFEELSAVSARVEDVRGVIQRAR
                     DRLGGNGRRTILFLDEIHRFNKAQQDALLPAVEDGLVTLIGATTENPYFEVNSALLSR
                     CAVYELEALSEDELLAVIQRGAAALDAEVAPAVAAAVAARAGGDARSALATLELAWET
                     ARAERETLAERHVADAARKRPLRYDRAADQHYDYASAFIKSMRGGDADAAIYYLAAML
                     EGGEDPRFIARRMVILASEDVGNADARALLVAVAAANAVEHVGLPEAQLNLAQAAAYL
                     ATAPKSNASAVAIWEARRDVRQHGNVEPPAMLRDAHYRGAATRGHGEGYVYPHDDAAG
                     FDVDYLPEKLRSAAEGRSRGRYYRPSRQLEGVDLRAVAVFLREIGERSAAEKVDALDR
                     AGRLGASEAVSDDG"
     gene            2153..2395
                     /locus_tag="AC150V_04115"
     CDS             2153..2395
                     /locus_tag="AC150V_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762139.1"
                     /translation="MEQIVEMRRQLDRAIAQRYALWQAPAVDRSNAIEKVELTIGRLW
                     QELRATRAEALNRPREEIIVAARAEDRLDRGLTRRR"
     gene            2435..2614
                     /locus_tag="AC150V_04120"
     CDS             2435..2614
                     /locus_tag="AC150V_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HIR3762140.1"
                     /translation="MASDQAKGKAKQAEGRAQEAWGNLKDEASDALEKAKDKAHEWQE
                     DAVKDEEDDDAGRSS"
     gene            2646..>3458
                     /locus_tag="AC150V_04125"
     CDS             2646..>3458
                     /locus_tag="AC150V_04125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3762141.1"
                     /translation="MPHPIVIVGGGLASARLVRAYRDAGGERRIVLISADDEPPYHRP
                     PLSKGYLRGDTDRRDVAVEQRGFYRELGVELRLGTVADSLDTESRLVRLGSGEDVEYS
                     DLVLATGARPRRLEVPGASLENVFHLRTLADSSAIMEAARQGSRALVVGGSFIGTEVA
                     ASLCQRGLEVTVVHQGDGLHDRLALPELSGFLGDLFTRVGVDVRLHEEVVALSGDDRV
                     SGAELASGEQIQADLVVVGIGVEPAVEIARGTRIDVDDGIVVDERLRTSVEQV"
BASE COUNT          565 a         1170 c         1243 g          480 t
ORIGIN      
        1 cgattatgcg ggcgacgact cggtagtggc gcacgcgcca ggcctgaacc cactcgtcga
       61 gctcgatcag cgactcggcg agctggtaga gctcctcgtg gtcgcgaccg tggacgtaca
      121 gctccgcgag cgagagccca gcgtcctccc gggcagcgtt gaaggccgcc tcgagttgtg
      181 gcatcacgcg gcgcagctcg gcgaacccgg gcgagtcgaa gcccgatccg tggccgagta
      241 ccttgcggat ctcctgatac tcccacgggg acatctgctc gagcatgtcg agctggtcgg
      301 tgagatatct gaggcagagc gcgggccgcc gcaggagccg tagcgccgcc gcgatctgac
      361 cggcctccac gtgtcgagcc gcagcctggg cgtccgccgt ggccagcttc agccacagct
      421 cggaggactg gtgaacgacc tggaagagca gctcgtcgcg gtggacccat tcgcccgcgt
      481 ccttctgcaa ggagaggagc tcctccgtat tcagatagcg ctcgtaatcg ctccccgccg
      541 tgccgggcag catcggttga gagaagtcct cttccgcttg cccggccatg cgcggagcat
      601 actccccgtc gatggacgat ctgtttgccg actccgcgcg cgagcagcag gcgacgaccg
      661 caccgttgcc ccagaggatc aggccccgag cgctcgacga gctggtcggt cagcgccacg
      721 tgctgggcga gggatcggcg ctccggaccg cgatcgagca ggaccggccg ccgtcgatga
      781 tcctccacgg cccgccgggc accggaaaga cgacactcgc gcggatcgtc gccgagcgca
      841 ccggcgccga gttcgaggag ctctcggcgg tgtcggcgcg cgtcgaggac gtccggggcg
      901 tgatccagcg agcgcgcgac cgcctgggcg gaaacggtcg ccgcacgatc ctcttcctcg
      961 acgagatcca ccgcttcaac aaggcgcagc aagacgccct gctccccgcc gtggaggacg
     1021 gcctggtgac actgatcggc gcgacgacgg agaacccgta tttcgaggtc aactcggcgc
     1081 tgctgtcgcg ctgcgccgtc tacgagctcg aggcgctctc ggaagacgag ctgctggccg
     1141 tgatccagcg gggggccgcc gccctggacg ccgaggtcgc accggccgtc gctgccgcag
     1201 tcgccgcccg cgccggcggg gacgcacgca gtgctctcgc caccctcgag ctcgcatggg
     1261 agacggcgcg cgcggaacgc gagactctcg ccgagcgcca cgtggcggac gctgcgcgca
     1321 agcgccccct ccgctacgac cgcgccgccg accagcacta cgactacgcc tcggcgttca
     1381 tcaagtcgat gcgcggcggc gacgcggacg ccgcgatcta ctacctggca gcgatgctcg
     1441 agggcgggga ggatccgcgc ttcatcgctc ggcgcatggt gatcctcgca tcggaggacg
     1501 tgggcaacgc ggatgcccgg gctctgctcg tggcggtcgc ggctgcgaac gcggtggagc
     1561 acgtcgggct gcccgaggcg cagctgaacc tcgcccaggc cgctgcctac ctcgccacgg
     1621 cccccaagtc gaatgcgtcc gcggtcgcga tctgggaagc gcgccgcgac gtccggcagc
     1681 acggcaacgt cgagcccccg gcgatgctgc gggatgccca ctaccggggc gctgccacgc
     1741 ggggccacgg ggaggggtac gtgtacccgc acgacgacgc cgccggcttc gacgtcgact
     1801 acctcccgga gaagctgaga tcggccgccg aaggacgctc tcgcggtcgg tactaccggc
     1861 cgtcgcgtca actcgaggga gtcgatctgc gagccgtggc cgtcttcctg cgcgagatcg
     1921 gggagaggag cgcggccgag aaggtggatg ccctcgaccg cgccggacgc ctcggcgcga
     1981 gcgaggctgt gtcggacgac ggctagcccc tgccgcagca ttctcggccg acaaccacag
     2041 cggatcgacg cgtgaccggc tcgggtttcg cgatcccgct ccgtgggtag tagaggtcat
     2101 gtctgtcaac agctccactc cttcgagcag ctctctgcgg cgcagaagct cgttggagca
     2161 gatcgtcgag atgcgccgcc agctggaccg cgcgatcgcg cagcggtacg cgttgtggca
     2221 ggccccagcc gtcgatcgat cgaacgcgat cgagaaggtc gagctgacga tcggcaggct
     2281 gtggcaggag ctgcgcgcca cccgggcgga ggcgctaaac cggccgcgcg aggagatcat
     2341 cgtcgctgca cgagccgagg atcgcctcga ccgcggtctc acccggcgac gctgagcgcg
     2401 acctgagcgt aaaccgacga aaggagagag ggaaatggcc agcgatcagg caaagggcaa
     2461 ggcgaagcag gcagaaggcc gggcgcagga ggcctgggga aacctgaagg acgaggcctc
     2521 ggatgcgctc gagaaagcca aggacaaggc acacgagtgg caggaagacg cagtgaagga
     2581 cgaggaggac gacgacgccg gtcgctcgtc gtagagcgac acgacgacac cgagaggagg
     2641 acgctgtgcc ccacccgatt gtgatcgtcg gcggcggcct ggcgagcgcc aggctcgtgc
     2701 gcgcctaccg agatgccggc ggcgagcgca ggatcgtgct gatctcggcc gacgacgagc
     2761 cgccgtatca caggccgcca ctctcgaagg gctatctacg cggcgacacc gaccgtcgcg
     2821 acgtcgccgt cgagcaacgc ggcttctatc gggagctcgg ggtcgagctt cggctcggca
     2881 cggtggccga ctcgctcgac accgaaagcc gactcgtgcg tctcggaagc ggcgaggacg
     2941 tcgagtacag cgatctcgtc ctggcgacgg gcgctcggcc gcgccggctc gaggttcccg
     3001 gcgcgagcct cgagaacgtg ttccacctgc gcacgctcgc cgactcgtcc gcgatcatgg
     3061 aggcggctcg gcaggggagc cgggcgctcg tcgtcggcgg cagcttcatc ggcacggagg
     3121 tcgccgcatc gctctgccag cgtggactcg aggtgacggt cgtgcatcag ggcgatggct
     3181 tgcacgaccg gctcgcgctg cccgagctct cggggtttct cggcgacctc ttcacgcgcg
     3241 ttggggtcga cgtgaggctg cacgaggaag tcgtcgcgct cagcggcgac gatcgcgtgt
     3301 cgggagccga gctcgcgtcg ggcgagcaga tccaggccga tctcgtcgtc gtcggcatcg
     3361 gtgtggagcc ggccgtcgag atcgcacgtg gtacccgcat cgacgtcgac gacgggatcg
     3421 tcgtcgacga gcgcctccgg acgagcgtgg agcaggtg
//
LOCUS       DBNGNK010000144         2029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1958325,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000144 DBNGNK010000000
VERSION     DBNGNK010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1958325"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC150V_04130"
     CDS             complement(<1..402)
                     /locus_tag="AC150V_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762142.1"
                     /translation="MIRRTIRDFIRAAERAEPEDQWTMLCFLAGRGVELSRDELNGSL
                     RRAELLLASGGDPRRKLELYGRAVTAVAEDLDSQPARAQLQEGLLALRSEAEGLRGAS
                     EALRLLTRDPDLAWQCFACALVAEELAAEGAE"
     gene            complement(409..1218)
                     /locus_tag="AC150V_04135"
     CDS             complement(409..1218)
                     /locus_tag="AC150V_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3762143.1"
                     /translation="MRTFETPGPLSLHVALQRGEVEIETADTTRTEVDVTALDGDPSD
                     ADAVRVEARERPGGLVEIHIEHPKKRSFLGIGAPQLRVRVRCPNGAGAQVHTGSADVR
                     LAGTYGDVALKTASGHSSLDVVEGACKISTASGDIDLLEGHGPIGVNSASGDLTVGIA
                     TGPLTASLVSGDVRVREARSTLTGNTVSGDVTVDASAGDVQLSSVSGDVVLGVRSGQQ
                     LWIDARSVSGTLRSELETGDAAVSHGEPVVELKVRTVSGDISIVRAAPAMV"
     gene            complement(1370..1834)
                     /locus_tag="AC150V_04140"
     CDS             complement(1370..1834)
                     /locus_tag="AC150V_04140"
                     /inference="COORDINATES: protein motif:HMM:NF017355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HIR3762144.1"
                     /translation="MQTSNHVRAIQSDLAAAASLGDESIAEAGRRLSEALESSLHLRL
                     LDLLTEAAADVSASLPLGHVEVRVAGREPELVFVAAEAEAEEPPPAGAEQLNARITLR
                     LPEVTKTAIEAAAAAEGVSTNAWIVRALIRATEPRADRTVRTGNRIQGFAHS"
BASE COUNT          322 a          714 c          680 g          313 t
ORIGIN      
        1 ctccgccccc tcggcggcga gctcttcggc gacgagtgca caggcgaagc actgccaggc
       61 aagatccgga tcgcgggtga ggagacgaag ggcctcggaa gccccacgaa gtccctccgc
      121 ctcgctacga agcgccagca gcccctcctg aagctgcgcc cgcgcgggct gtgagtccag
      181 gtcctccgcc acggcggtca ccgcacgtcc gtagagctcc agcttccggc gcggatcgcc
      241 gcccgacgca agcaggagct cggcgcggcg tagcgacccg ttcagctcgt cgcggctcag
      301 ctcgacgcca cggcccgcga ggaagcagag catcgtccat tgatcctcgg gctccgccct
      361 ctccgcggca cgaatgaaat cgcgaatcgt acgacggatc acgagcgctc agaccattgc
      421 gggcgccgcc cggacgatcg agatgtcccc actgaccgtg cgaaccttca gctcgacgac
      481 aggctcccca tgcgagactg ctgcgtcgcc ggtctccagc tcggaccgca gtgtgccact
      541 gaccgatctg gcatcgatcc agagctgctg tccgctgcga acgccgagga cgacgtcgcc
      601 cgagacgctg ctcagctgga cgtcgccagc gctggcgtcg actgtcacgt cgccggagac
      661 ggtgttgcct gtcagcgtgc tccgcgcctc gcgcacgcgc acgtcaccag agacgaggct
      721 cgcggtgaga ggacctgtcg ctatgccgac ggtcaggtca cccgacgcgc tgttgacgcc
      781 gatcggaccg tgcccctcga gcaggtcaat gtcgccactt gcggtgctga tcttgcacgc
      841 tccctcgacg acgtcgagag aactgtggcc gctcgcggtc ttgagcgcga cgtcaccgta
      901 ggtgccggcc agacggacat cggccgagcc ggtgtgaacc tgcgccccgg ccccgttcgg
      961 gcagcggacg cgaacgcgca gttgtggcgc gccgatgccc aggaagctcc gcttcttggg
     1021 gtgttcaatg tggatctcga ccaggccgcc ggggcgttca cgcgcctcga cgcgaacggc
     1081 gtcggcgtcg gacgggtcgc cgtcgagggc ggtgacgtcg acctctgtgc gcgtcgtgtc
     1141 cgcggtctcg atctccacct caccacgctg cagggcgacg tgcaggctga gagggccggg
     1201 tgtctcgaag gtccgcacgg cgtcacctgg cgcggcgcga cggatcggct actcgccgga
     1261 tccggacgaa gcggacatcg tgcggacgct ccgccggccg gcgctgctcg gccgccgggc
     1321 cttcgatgta tccgaacaat gagggataaa gcgccattct tggctccttt cagctgtggg
     1381 cgaagccctg gatgcggttg ccggtacgaa cagtgcgatc ggcacgcggc tcggttgctc
     1441 ggatcagcgc gcgaacgatc cacgcattgg tcgagacacc ctcggctgcg gccgcggcct
     1501 cgatggcggt cttcgtcacc tccggcagcc ggagcgttat ccgcgcgttc aactgctctg
     1561 cgcccgccgg cggaggctcc tcggcttcag cctcggcagc gacgaagacg agctccggct
     1621 cgcgcccggc gacgcgaacc tcgacgtggc cgagcgggag cgacgcggag acgtcagccg
     1681 ccgcctcggt gaggagatcc agaagccgga gatgcagcga cgactccagc gcttcgctga
     1741 gcctacgacc cgcctctgcg atcgactcgt cgccgagcga ggcggcggcg gcgaggtcgg
     1801 actggatcgc cctgacgtgg tttgacgtct gcatggtggc aattatgaca tcgaaaagac
     1861 atcatgtcaa gtctttctga tgtcatcgct ggcgccgctg ctaccctgcg cctcgccgga
     1921 acccgtgcct ggtcccggct gcctcgcacg ccttcagggg tgaaccggcg ccgcgacgca
     1981 cagcaggccc atgacgaact cgcagcacgc cgcaggagga cgaaacccc
//
LOCUS       DBNGNK010000145         7331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1312986,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000145 DBNGNK010000000
VERSION     DBNGNK010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7331
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1312986"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="AC150V_04145"
     CDS             complement(<1..1425)
                     /locus_tag="AC150V_04145"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF021507.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HIR3762145.1"
                     /translation="MRRRLLIPALLALIAAVFAAPAGGQQKSVLMPGVLYQRAVQFTP
                     NGPVALHVVTGPKPTGLYALKPVLSNETILGRETVTQMQKRISRKATTVGTNGDLFAW
                     ENGRPSGILMRERVLDSAPLGDRSSVGVTNTGFLDVQRVNFFGTWFGAAKSHPLQDVN
                     EEPPPNGVSLFTPVWGDRTPAVKNAVQVVLAGFPAATADEELPGIVTAVVPSGAGTVP
                     IPRGGAVLVGHGSARRTLLREAPIGTQVFVRLILRPNWESVTDAIGGGPIIVRDGAPV
                     FRANEAFLETQLGPRNPRTAIGQRRDGRVLMVVTDGRQPEWSVGMTNFELGQTLARLG
                     AVTASALDAGGSTTLAFDGELLNQPSDAGGERSISTALMLMYYGVILQPPAKKVVSPN
                     GDGVDERQVLRYKLVRPATVTVSMTAPDGRVVYEETVDRKVGRYRVPFPPPLPPPIEA
                     PAPPAPAPPPAEPPPAEPPPAEPPP"
     gene            complement(1559..2665)
                     /gene="mqnC"
                     /locus_tag="AC150V_04150"
     CDS             complement(1559..2665)
                     /gene="mqnC"
                     /locus_tag="AC150V_04150"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR3762146.1"
                     /translation="MTSLASGSAVREALDKALDGERIADDDAIALLRSRDLLAVGRVA
                     DQIRGRKSDPSRVTFIVDRNINYTNVCYTDCDFCAFYRSPGDTREGYLLPKSVIYKKI
                     EETIAIGGTGVLMQGGHHPDLGIDYYEDLFSSIKARYPIHLHCLSPPEIQHVSRRSKL
                     TIWETLARLRDAGLDSVPGGGAEILVDRVREIIAPKKTKSDEWLDVMRHAQRLGMSTT
                     ATMMYGHVETLEERVEHMRRIRELQDETRGFRAFISWTFQRDGNRLDEVVRDEDMPTS
                     FDYLLTQAVSRIYLDNVDHIQSSWVTQGLKVGQVALGFGADDMGSVMLEENVVSAAGT
                     THRTSTDELVHLIRALGKTPVQRDTLYRDVRAFD"
     gene            complement(2662..3420)
                     /locus_tag="AC150V_04155"
     CDS             complement(2662..3420)
                     /locus_tag="AC150V_04155"
                     /EC_number="4.2.1.151"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthetic enzyme MqnA/MqnD family
                     protein"
                     /protein_id="HIR3762147.1"
                     /translation="MRLGRISYVNMAPVFHRLKADVEEVAGVPTELNGLLLAGELDLA
                     PISSIEYARHADRLRLLPRLCVSSEGAVDSIQLVTRVPLGNVRTVAATPESATSVVLV
                     RILLPSAEIVTLDEPADATLLIGDAALRSAFEDPTPHYDLGRLWLERTGLPMVFAVWA
                     APEPVADGLSELEHALVASVRLARAEPEQLAYEASERYGYPAGFLARYFEKLRYSFGP
                     RERAGLYTFLEMARDVGELDQVPELRFTREAVSA"
     gene            complement(3417..4361)
                     /locus_tag="AC150V_04160"
     CDS             complement(3417..4361)
                     /locus_tag="AC150V_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3762148.1"
                     /translation="MSDPLELTPAQRESDSLDWTFGGTWPYEPRWLFSDGIRLHYVDE
                     GPGEGEPVVLLHGNPTWSYLYRNFIPGLTGAGFRSIALDQLGFGRSDKPKRQSEYTIQ
                     RHARHFAALMNELSIEGATLVMQDWGGPIGLSWAVDNPDGVKRLVLFNTFTGSLPAGS
                     KPPAPFRAIRAPGSGELLVKGMHVFVRRLLFKDGVAHPERLGENEKAAYLQPHPSWDS
                     RTGVLAYPRLIPWDEGNPTRPLGEHIDANLDRLADKPVLICWPLRDPAFTKPVLEMWR
                     ARFPDAEVHEIEDASHYVQEDAHERIVPWLVDFLGRTS"
     gene            complement(4358..5464)
                     /gene="mqnE"
                     /locus_tag="AC150V_04165"
     CDS             complement(4358..5464)
                     /gene="mqnE"
                     /locus_tag="AC150V_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HIR3762149.1"
                     /translation="MAVLEQATLERIREKVEAGERLDADDGVTLLESDDLLVLGELAD
                     LARRRRGGSDDVFFVQNLYLNQTNVCRVKCKFCAFAATRKQEHAYTLTTEELVEDALQ
                     QRELTGFTEIHMVNGENPHVDFDFYVDTIRSLHDAMPDVHLKCYTASEIHHMTTLSGL
                     SHEEVLRELRDAGLGSLPGGGAEIFADRVRRLVAPGKEHPEFWFQTHDAAHRLGVPTH
                     CTMLYGHVETYEERIDHLLRLRDQQDETGGFLAFIPLAFHPENTVFERRGFRHTTGAD
                     DLKMLAVSRLMLDNVPNIKAYWIMMGMPLAAIALHFGANDVQGTVVREEIFQAAGASA
                     GTEQKVEQLVRFVRAAGRVPVQRDTLYNELRRWE"
     gene            complement(5464..6366)
                     /locus_tag="AC150V_04170"
     CDS             complement(5464..6366)
                     /locus_tag="AC150V_04170"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR3762150.1"
                     /translation="MSVLETVRAAEGLDGYLAAVEGRLTAGVASYPGLVSAVGAEALT
                     AGGKRLRPVLSFLSAPEDAPPPVAAGTAVELVHMATLVHDDLIDGARVRRGRAAAWAQ
                     HGVGPARAAGDYLFARAFAELTRADDPDGVAVLADACLALARGEAMQRVQRHDPDTTV
                     EDYVERCALKTGRLFEAACRLAGGSGEYGLLLGIAFQIADDVLDCSGDTIETGKVPGA
                     DLRDGTPTLPLLLAAREDPVVHAALAGGPLDGVLVRVAATGALERSQGIALDYAHKAR
                     ASLDGVGRHEELEALADAVVERNT"
     gene            complement(6363..7067)
                     /locus_tag="AC150V_04175"
     CDS             complement(6363..7067)
                     /locus_tag="AC150V_04175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR3762151.1"
                     /translation="MGRMTYETGTLTPDGVRTMFDRIAPVYDAMNRLMTGGLDRSWRR
                     LTVEAVVGPGNRVLDACCGTGDLAVEAEREGGFVTGLDFAPAMLRRARSKSSTIDWVE
                     GDLLALPFEDETFDVATVGFGVRNVADLELALRELRRVLRPGGRLAILEITQPRGLLR
                     PFFRLWFDVLVPAAGRLLPGGAAYTYLPSSVRRFPDVDSLAALLRATGFGVVRARLLG
                     GTIVALHVAVAGEEHA"
     gene            complement(7090..>7331)
                     /locus_tag="AC150V_04180"
     CDS             complement(7090..>7331)
                     /locus_tag="AC150V_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762152.1"
                     /translation="VFAQSGCLNCHVYTGEGNANLGAPELTAIGETRDAQYFIDYVAN
                     PAAFGNQVMGSYSYLGQENLEALGEFLAQSKGAGG"
BASE COUNT         1130 a         2513 c         2567 g         1121 t
ORIGIN      
        1 cggcggtggc tcggcggggg gcggctcggc ggggggcggc tcggccggcg gcggagcggg
       61 ggcgggcggc gctggcgctt cgatcggcgg cgggagcggc ggcggaaacg gaacgcggta
      121 gcgtccgacc ttccggtcaa ccgtctcctc gtagaccacc cggccgtcgg gcgcggtcat
      181 cgagacggtg accgttgcag gccggacgag cttgtagcgg agcacctgcc gctcgtcgac
      241 gccgtcgccg ttcggcgata cgaccttctt ggccggcggc tggaggatca cgccgtagta
      301 catgagcatc agggcggtcg agatcgacct ttccccgccc gcatccgagg gctggttgag
      361 cagctcgccg tcgaaggcga gcgtcgtcga gccgcccgcg tcgagcgccg aggcggtgac
      421 cgctccgagc cgcgccagcg tctggcccag ctcgaagttc gtcatcccga cgctccattc
      481 gggctgacgt ccgtcggtga cgaccatcaa gacccgccca tcgcgccgtt ggccgatcgc
      541 ggtgcgcggg ttgcgcggcc cgagctgggt ctcgaggaac gcctcgttcg cgcgaaacac
      601 cggagcgccg tcccggacga tgatgggacc gccgccgatc gcgtcggtca ccgactccca
      661 gttcgggcgc agaatcagcc gcacgaagac ctgcgtgccg atcggggcct cgcggagcag
      721 agtccgtcgc gcggagccgt gcccgacgag caccgctcct ccgcgcggga tggggacggt
      781 gcccgcaccg ctggggacga cggccgtcac gatgcccgga agctcctcgt ccgcggtcgc
      841 agcggggaag cccgcgagca cgacctgcac cgcgttcttc accgccggcg tgcgatcgcc
      901 ccacaccggg gtgaagagcg agacgccgtt ggggggtggt tcctcgttca cgtcctgaag
      961 cgggtggctc ttggccgccc cgaaccacgt gccgaagaag ttgacccgct gcacgtcgag
     1021 gaagcccgtg ttcgtgacgc ccacgctcga gcggtcgccc aacggggcgc tgtcgagcac
     1081 gcgctcgcgc atcaggatcc cgctcggccg gccgttctcc caggcgaaca agtcgccgtt
     1141 cgtaccgacc gtcgtcgcct ttcgggatat gcgcttctgc atctgcgtga cggtctcgcg
     1201 gccaaggatc gtctcgttcg agagcaccgg cttgagcgcg tagagcccgg tcggcttggg
     1261 tcccgtcacg acgtggagcg cgacagggcc attgggggtg aactgcacgg cgcgctggta
     1321 gagcacaccc ggcatgagca ccgacttctg ctgacccccg gcgggagccg cgaagacagc
     1381 ggcaatcagc gcaagcagag cagggataag gaggcggcga cgcacgaccc gcaccgaggg
     1441 tagcgaaggc cgctgcgggc cccaaccctc acttacacgt tccaaacctg tctctgtgca
     1501 ggttatttag aactggatga ccattccgta acgcctcggc cgacggaccg gccgggcgct
     1561 agtcgaaggc gcgaacgtcg cggtagagcg tgtcgcgctg caccggcgtt ttgccgagcg
     1621 cgcgaatgag gtggacgagc tcgtccgtgg atgtgcggtg cgtcgtgccc gcggccgaga
     1681 cgacgttctc ctcgagcatc accgagccca tatcgtcggc gccgaagccg agcgcgacct
     1741 ggccgacttt caatccctgc gtcacccagg aggactggat gtggtcgacg ttgtcgaggt
     1801 agatgcgtga gacggcctgg gtcagcaggt agtcgaacga ggtgggcatg tcctcgtcgc
     1861 ggacgacctc gtcgaggcgg ttgccgtcgc gctggaacgt ccaggagatg aaggcgcgga
     1921 agccgcgggt ctcgtcctgc agctcgcgga tgcggcgcat gtgctcgacg cgctcctcga
     1981 gcgtctccac gtggccgtac atcatcgtcg cggtcgtcga catccccagc cgctgcgcgt
     2041 ggcgcatgac gtccagccac tcgtccgact tcgtcttctt cggcgcgatg atctcccgga
     2101 cgcggtcgac caggatctcg gcgccgccgc cgggcaccga gtcgagcccg gcatcgcgca
     2161 ggcgggcgag cgtctcccag atggtcagct tcgaccggcg cgagacgtgc tggatctcgg
     2221 gcggcgagag gcagtgcagg tggatcgggt agcgcgcctt gatcgagctg aagaggtcct
     2281 cgtagtagtc gatgccgagg tcggggtggt ggcctccttg catcaggacg ccggtgccgc
     2341 cgatcgcgat cgtctcttcg atcttcttgt agatgaccga cttcggcagg agatacccct
     2401 cacgcgtgtc tccggggctc cggtagaagg cgcagaagtc gcagtccgtg tagcagacgt
     2461 tcgtgtagtt gatgttccgg tcgacgatga aggtgacgcg gctcggatcg ctcttccggc
     2521 cgcgtatctg atccgccacc cgtcccactg cgaggaggtc acgggagcgg aggagtgcga
     2581 tcgcgtcgtc gtcggcgatg cgctcgccgt cgagcgcctt gtcgagggct tcacggacgg
     2641 ccgagcccga ggcgagagac gtcacgcgct caccgcctca cgcgtgaaac gcagctccgg
     2701 cacctggtcg agctcgccga cgtcgcgtgc catttcgagg aacgtgtaga ggccggctcg
     2761 ctcacgcggc ccgaagctgt aacgaagctt ctcgaagtag cgggcgagga agccggcggg
     2821 gtagccgtag cgctcgctcg cctcgtacgc gagctgctcg ggctcggcgc gcgcgagccg
     2881 taccgacgcg acgagggcgt gctcgagctc cgacagcccg tcggcgacgg gctcgggcgc
     2941 agcccacacc gcgaagacca tcggtaggcc cgtccgctcg agccagagcc gccccaggtc
     3001 gtagtgcggc gtcggatcct cgaacgcact ccgcagggcc gcgtcgccga tcagcagcgt
     3061 cgcatccgcc ggttcgtcga gcgtgacgat ctcggcgctc ggtagcaaga tcctcaccag
     3121 cacgacggat gtcgccgact cgggggtcgc ggcgaccgtg cggacgttcc cgagcggaac
     3181 gcgcgtgacc agctggatcg agtccaccgc accctcggac gagacgcaga gcctcggcag
     3241 caggcgaagc cggtcggcgt gccgcgcgta ctcgatcgac gagatcggcg caaggtcgag
     3301 ctccccggcc aggagcaggc cgttcagctc ggtcggaacg ccggctacct cctcgacgtc
     3361 ggccttgagc cggtggaaga cgggggccat gttgacgtag gagatccgcc ccagtctcaa
     3421 ctcgtcctcc cgagaaagtc gaccagccag ggcacgatcc gctcgtgcgc gtcctcctgt
     3481 acgtagtggg acgcgtcctc gatctcgtgt acctcggcgt cggggaagcg cgcccgccac
     3541 atctccagca ccggcttcgt gaaggcggga tcgcgcagag gccagcagat gagcaccggc
     3601 ttgtcggcga gccggtcgag gttcgcatcg atgtgctcgc cgagcgggcg ggtcgggttg
     3661 ccctcgtccc acggaatcag ccggggatag gcgagcacgc cggtgcggct gtcccagctc
     3721 ggatgcggct ggaggtacgc cgccttctcg ttctcgccga ggcgctccgg gtgagcgacg
     3781 ccgtccttga agaggagcct gcgcacgaac acatgcatcc ccttgacgag gagctcgccc
     3841 gacccgggcg cccggatcgc gcggaacggc gccggcggct tcgagccggc ggggaggctg
     3901 ccggtgaagg tgttgaagag gacgagccgc ttcacaccgt cggggttgtc cacggcccag
     3961 gacagcccga tcggcccgcc ccagtcctgc atcacgagcg tcgcgccttc gatcgacagc
     4021 tcattcatga gcgccgcgaa gtgcctcgcg tggcgctgga tcgtgtactc gctctgtcgc
     4081 ttcggcttgt ccgagcggcc gaagccgagc tgatccagcg cgatcgaccg gaagccggcc
     4141 ccggtcaggc cggggatgaa gttgcgatac aggtaggacc aggtcgggtt cccgtggagc
     4201 aggacgacgg gctccccctc ccccgggccc tcgtcgacgt agtggagccg gatcccgtcg
     4261 ctgaacagcc accgcggctc gtacggccat gtgcccccga acgtccagtc gagcgagtcg
     4321 gactcgcgct gggccggagt cagctcgagt gggtcgctca ttcccaccgc ctgagctcgt
     4381 tgtagagcgt gtcccgctgc accggcaccc ggcccgccgc ccgcacgaag cgtacgagct
     4441 gctcgacctt ctgctccgtc ccggcggaag ctcccgcggc ctggaagatc tcctcacgca
     4501 ccacggtgcc ctgcacgtcg ttggcgccga agtgcagcgc gatggctgcc agcggcatcc
     4561 ccatcatgat ccagtaggcc ttgatgttcg gcacgttgtc gagcatcagg cgcgagacgg
     4621 cgagcatctt caggtcgtcc gcgccggtcg tgtgtcggaa cccgcgccgc tcgaacacgg
     4681 tgttctcggg atggaaggcg agcggaatga aggcgaggaa gcccccggtc tcgtcctgct
     4741 ggtcgcgcag ccggaggaga tggtcgattc gctcctcgta cgtctcgacg tggccgtaga
     4801 gcatcgtgca gtgggtcggg acgccgagcc ggtgggcagc gtcgtgcgtc tggaaccaga
     4861 actcgggatg ttctttgccg ggtgcgacga ggcgtcgcac gcggtcggcg aatatctccg
     4921 cacctccgcc cggaagggag ccgaggccgg cgtcgcgcaa ctcccgcaga acctcctcgt
     4981 ggctgagccc ggacagcgtt gtcatgtggt ggatctcgga ggccgtgtag cacttgaggt
     5041 gaacgtccgg catcgcgtcg tgcagcgagc ggatcgtgtc gacgtagaag tcgaagtcaa
     5101 cgtgcggatt ctcgccgttg accatgtgga tctcggtgaa cccggtcagc tcacgctgct
     5161 gcagcgcgtc ctccacgagc tcctcggtcg tcagcgtgta ggcgtgctcc tgcttgcggg
     5221 tggcggcgaa ggcgcagaac ttgcatttca cgcggcacac gtttgtctgg ttcaggtaca
     5281 ggttctgcac gaagaagacg tcgtcgctgc cgccgcggcg ccgacgtgcg aggtcggcca
     5341 gctccccgag aacgagcagg tcgtcggact cgagaagcgt gacaccgtcg tccgcgtcga
     5401 ggcgctcgcc cgcctccacc ttctcgcgga tcctctccag cgtcgcctgc tccagaacgg
     5461 ccactaggtg ttcctctcca cgacggcgtc cgccagagcc tccagctcct cgtggcgacc
     5521 tacgccgtcg aggctcgcac gggccttgtg agcgtagtcg agcgcgatcc cctgcgatcg
     5581 ctcgagcgcg ccggtggccg cgacccggac gagcacgccg tcgagcggtc caccggcgag
     5641 ggcggcatgc acgaccggat cctcgcgcgc cgcgagcaga agcgggagcg tcggcgtgcc
     5701 gtcacgcagg tccgctcccg gcaccttccc cgtctcgatc gtgtcacccg agcagtcgag
     5761 cacgtcgtcg gcgatctgaa acgcgatgcc cagcagcagg ccgtactcgc cgcttccgcc
     5821 ggcgagcctg caggcggcct cgaacagccg ccccgtcttg agcgcgcacc gctcgacgta
     5881 gtcctcgaca gtcgtgtccg ggtcgtgcct ttggacgcgt tgcatggcct ccccccgggc
     5941 gagcgcgaga caggcgtccg ccagcaccgc gacgccgtcg ggatcgtcgg cacgcgtgag
     6001 ctcggcgaag gcccgcgcaa agaggtagtc gcccgcggca cgcgcaggcc cgacaccgtg
     6061 ctgcgcccag gcggcggcgc ggccgcgccg cactcgagcg ccgtcgatca agtcgtcatg
     6121 cacgagcgtc gccatgtgaa cgagctcgac cgccgtaccg gccgctaccg gaggaggcgc
     6181 gtcctccgga gcggagagga acgagagaac cgggcgcagg cgcttgccgc cggccgtgag
     6241 cgcttcggcg ccgacggccg agacgagccc cggatagctc gcgacgccgg ccgtgagccg
     6301 cccctcgacg gcggcgaggt acccgtccag gccctccgcg gcccgcacag tctccaacac
     6361 gctcacgcgt gctcctcgcc ggcaacggcc acgtgcagcg cgacgatcgt gccgccgagc
     6421 aggcgcgccc gaacgactcc gaagcccgtg gcccggagca gcgcggccaa actgtcgacg
     6481 tccggaaaac gacgcacgct agatggaaga taggtgtagg cggcgccccc gggtagcagt
     6541 cgcccggccg cgggcacgag cacgtcgaac caaagcctga agaaggggcg gagcagaccg
     6601 cgcggctgcg tgatctcgag aatcgcgagc ctgccccccg gccgcagcac acggcggagc
     6661 tcgcgcagcg ccagctcgag gtcggcgacg tttcgcacgc cgaagccgac ggtggccacg
     6721 tcgaacgtct cgtcttcgaa cggaagcgcc agcaggtcgc cctcgaccca gtcgatcgtg
     6781 ctcgacttgc tgcgtgcgcg ccggagcatc gcgggcgcga agtcgagacc tgtaacaaag
     6841 ccgccttccc gctcggcttc gacggcgaga tcgccggtgc cgcaacacgc gtcgagcacc
     6901 ctgttgcccg gcccgaccac cgcctcgacc gtcagccgtc gccacgagcg gtcgaggcca
     6961 ccggtcatca gccggttcat ggcgtcgtag acgggcgcga tccggtcgaa catcgtcctc
     7021 acgccgtccg gcgtgagcgt gcccgtctcg tacgtcatcc gccccatcgg ggcagcagcc
     7081 tagtgggtgc tagccgccgg cgcccttgga ttgcgcgagg aactcgccca gggcctcgag
     7141 gttctcctga cccaggtacg agtaggagcc cataacctgg ttgccgaacg cggccgggtt
     7201 ggcgacgtag tcgatgaagt actgcgcgtc gcgggtttct ccgatcgcgg tgagctccgg
     7261 tgccccgagg ttggcgttgc cctcgcccgt gtagacgtgg cagttgagac agcccgactg
     7321 ggcgaaaacg a
//
LOCUS       DBNGNK010000146         3255 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_310272,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000146 DBNGNK010000000
VERSION     DBNGNK010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_310272"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="AC150V_04185"
     CDS             complement(<1..1187)
                     /locus_tag="AC150V_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3762153.1"
                     /translation="MRAETVIVTHGNTDFDALASQLAARRLYPGSTVALQGSLNRNVR
                     ELVRLHAEELDVVEGSRLELDAIKRLVVVETVVFDHHTGELPEWVDPETAVISHDGAL
                     TTTMVGILLERELEPTPLEATLFALGIHEDTGSLTYLTATQRDADALAWCMRHGASQE
                     LVLEYLHAPLSASERGLLHALEEVIEPVDAGGEELLLAALAWPEYVDGVSNLAHKLVD
                     LTDCRALVLLVEMEDRVVAVARSRSERIDAAALAAGLGGGGHEGAASAIARIPLADAR
                     ARVLAAVPDALREPRRARDVMSTPVRSVAPDDSVSQAMAMCQRHGQSGVFVVDGEGRL
                     LGGVRREDLDKAIGHDLAHAPVKAVMSGRLTTCGEEATLAELQRLVTVAEDGRVAVVR
                     DER"
     gene            1396..2142
                     /locus_tag="AC150V_04190"
     CDS             1396..2142
                     /locus_tag="AC150V_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR3762154.1"
                     /translation="MSSPSVLAVFLAALLTALATGLGAIPLLFIRSAGRRALGAANAV
                     AAGVMLGASLSLVIEGVDRSAWKTVAGAVVGAAFVLVAERFLGHERENLALGSLRGDD
                     ALKALLIVAIMTVHSAAEGIGVGSAFGGGATLGVLITVAIAIHNIPEGLAISLVLVPR
                     GTSVGAAAGWSIFSSLPQPLLAVPAFLFVETFEGILPAGLGFAAGAMVWMVSRGLLPE
                     ALAQTSARTVIGAGSIGFAAMVLFQLLLLG"
     gene            2188..3138
                     /locus_tag="AC150V_04195"
     CDS             2188..3138
                     /locus_tag="AC150V_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3762155.1"
                     /translation="MSTDAAATTKPATGRRLLPATVRGVGNYLADQGPQFAAAISYKA
                     IFSIIPLITFAITVVGVFLRNDERRTELIAEILDALPLSAEATEDLNRILSDIPSPWS
                     LLGLLSLLVVLWSASGVMSSIRVSLTVASDAPPEGSYPRRKLIDFALVVAATVFFVAA
                     ATLNIVSRTFAQWTDKILDAINADWLSGVVGPFISGFAVPAVLLTCAVVFIYWFVPPV
                     SGPWRYLVPGGILAGVSLQAIQIALAWWLKVSASADPVTGLAGGILGFLLTVYISANA
                     FLIGGELIYAWAGRPSAPPKGDSPSILEQIARKLEARIGS"
BASE COUNT          457 a         1175 c         1078 g          545 t
ORIGIN      
        1 atccgctcgt ctcgcaccac cgcgacacgc ccatcctcgg cgacggtgac gaggcgctgg
       61 agctcggcca gcgtggcctc ctcgccgcag gttgtcagac gaccgctcat gacggccttg
      121 accggagcat gcgccaagtc gtggccgatc gccttgtcga ggtcctcccg acgcactccg
      181 cccaacaagc gcccctctcc gtccacgacg aagaccccgc tctggccgtg gcgctgacac
      241 atggccatgg cctgggacac cgagtcgtcg ggcgcgaccg agcgcaccgg ggtcgacatg
      301 acatcgcgcg cacggcgcgg ctcgcgcaac gcgtcgggaa cggcggcaag gacgcgggcg
      361 cgagcgtcgg caagcgggat tcgcgcgatc gcggaggccg caccctcgtg gccgccgccg
      421 ccgagccccg ccgccaacgc cgcggcgtcg atccgttcgc tccggctgcg ggcgacggcg
      481 acgacccgat cctccatctc gacaaggagg acgagagcgc gacagtctgt gaggtcgacc
      541 agcttgtggg cgagattgga tacgccgtcg acgtactcgg gccaggcgag ggccgcgagc
      601 agcagctcct cgccccccgc atcgaccggc tcgattactt cctcgagcgc gtgcagaagt
      661 ccgcgttccg aggcagagag cggtgcgtgc aggtactcga gcaccaactc ctgtgaagcg
      721 ccgtgtcgca tgcaccaggc aagggcgtcc gcgtcgcgct gcgtcgccgt cagatacgtg
      781 agcgaccccg tgtcctcgtg gatcccgagc gcgaagaggg tggcttcgag cggtgtcggc
      841 tcgagctccc gctcgaggag aatcccgacc atcgtcgtcg tgagcgcgcc gtcgtgcgag
      901 atcacggccg tctcgggatc gacccactcg gggagctcac cggtgtgatg gtcgaagacg
      961 acggtctcga cgaccacgag ccgcttgatc gcgtccaact cgagccgcga cccttcgacc
     1021 acgtccagct cctcggcgtg gagtcgaacg agctcccgca cgttgcggtt gagcgagccc
     1081 tgcagtgcca ctgtggatcc cgggtacagc ctccttgctg ccagttgcga ggcgagggcg
     1141 tcgaagtccg tgttcccgtg ggtgacgatg acggtctccg cgcgcacccc gcccattctg
     1201 ccgacggcac gtgcggaaaa acaccgatgc cccggcccga gggcgctgag acgattcggt
     1261 cacgtggcga gtaactcgcc tggtgcagat gcaactcacc atgccgtggt gaggagcagg
     1321 cggggggtgg tcaccggtcg ggagccgccc cccgtcgtca tccgtctcgc caccgccgcc
     1381 acgtaggctc gaatcgtgtc ctcgccgtcc gtcctcgcag tcttcctcgc cgcgctgctg
     1441 accgcactcg cgaccgggct gggcgcgatt ccgctcctgt tcatccgctc ggccggccgc
     1501 cgtgcgctcg gcgctgccaa cgccgtcgcc gccggcgtga tgctgggtgc cagcctcagc
     1561 cttgtcatcg agggcgtcga tcgaagcgcc tggaagaccg tggccggagc cgttgtcggg
     1621 gcggcgttcg tgctcgtggc agaacgtttc ctcggccacg aacgcgagaa tctcgcactc
     1681 ggctcgctcc ggggcgacga tgcgctcaag gcgctcctga tcgtcgcgat catgacggtt
     1741 cactcagccg ccgagggaat cggcgtcggc tccgccttcg gcggcggggc gaccctcggg
     1801 gttctgatca cggtcgccat cgcaatccac aacatccctg agggactggc gatcagcctg
     1861 gtgctcgtcc cccgtggcac gagcgtcggc gccgccgctg gctggagcat cttctcgagc
     1921 ctgccgcagc cgctgctcgc cgtccccgcc ttcctcttcg tcgagacgtt cgaggggatc
     1981 ctgccggccg gcctcggatt cgcggccggt gcgatggtgt ggatggtctc acgcgggttg
     2041 ctgcccgagg ccctcgcgca gacgtccgcg cgaaccgtta tcggcgccgg ctcgattggc
     2101 ttcgccgcga tggtcctctt ccagctgctc ctgctcggct agcggcgtcc cccgcccgcc
     2161 gccactacgt ggcaatactg tcgctcgatg agcacggacg ccgccgcgac gaccaagcct
     2221 gccacgggcc gccgcctgct gccggcaacg gtacgcggag tgggcaacta cctcgccgac
     2281 caggggccac agttcgcagc cgcgatctcg tacaaggcga tcttctcgat catcccgctg
     2341 atcacgttcg cgatcacggt cgtcggggtc ttcctccgca acgacgagcg ccggaccgag
     2401 ctcatcgcag agatcctcga cgcgctgccg ctgagcgcgg aggcgaccga ggacctcaat
     2461 cgcatcctca gcgacatccc gtcgccgtgg agcctgctcg gcctcttgtc gctcctcgtc
     2521 gtcctctggt cggcgagcgg cgtgatgagc tcgatcaggg tctcgctgac cgtcgcgtcc
     2581 gacgcgccgc cggaggggtc gtatccaagg cgcaagctga tcgacttcgc gctcgtcgtg
     2641 gctgcgacgg tcttcttcgt cgccgccgcg acgctgaaca tcgtcagccg cacgttcgcg
     2701 cagtggaccg acaagatcct ggacgcgatc aacgccgact ggctgtcggg tgtcgtcggc
     2761 ccgttcatct ccggattcgc cgtccccgcc gttctcctga cctgcgcggt cgtgttcatc
     2821 tattggttcg tgccaccggt gagcgggccg tggcgctacc tcgtgcctgg cgggatcctc
     2881 gcgggcgtct cgctccaggc gatccagatc gccttggcct ggtggctcaa ggtgtcggcc
     2941 tcggcagatc cggtcacggg cctcgcgggc ggaatcctcg gcttcctgct gaccgtgtac
     3001 ataagcgcga acgcgttcct catcggcggc gagctcatct acgcgtgggc cggtcgccct
     3061 tcggcgccgc cgaaaggcga ctcgccgagc atcctcgagc agatcgcgcg aaagctcgag
     3121 gcccggatcg gctcgtgacg cgcccgtcta cagctgggcg tcgtcctcgc gaacggagaa
     3181 cgcgactcgc cagtcgtccc gtgactcgcc gtcgatcgag aggcgccact cgtagcgccc
     3241 gcccggctcc agcgt
//
LOCUS       DBNGNK010000147         5251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_524705,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000147 DBNGNK010000000
VERSION     DBNGNK010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5251
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_524705"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /gene="trpB"
                     /locus_tag="AC150V_04200"
     CDS             complement(<1..1004)
                     /gene="trpB"
                     /locus_tag="AC150V_04200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3762156.1"
                     /translation="MTTTYGSYGGRYVPETLIPALDELTDAWESAKEDRSFWDELDEL
                     LRTYAGRPTPLTLAERFSPGKRVYLKREDLLHTGAHKLNNALGQLVLARRLGKTRIIA
                     ETGAGQHGVATATACARFGLQCVVFMGAEDMRRQAPNVERMGLLGATVVPVEFGTRTL
                     KEATSEAIRDWITNVETTHYVIGSCVGPAPYPELVRELQAVIGREARAQILEAAGTLP
                     EVVVASVGGGSNAIGMFAGFLADDGVELVGVEAAGAASLGTGRAGVLHGSRSSILADE
                     DGQISDAHSISAGLDYPGVGPEHAMLAETGRATYVAATDRVAIEGFRRLSELEGIIPA
                     L"
     gene            complement(1001..2161)
                     /gene="trpF"
                     /locus_tag="AC150V_04205"
     CDS             complement(1001..2161)
                     /gene="trpF"
                     /locus_tag="AC150V_04205"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR3762157.1"
                     /translation="MGRFEEILARPGLGAIAEIKRRSPSAGDLRPGADPAGIAPAYAD
                     AGAAAVSVLVDERFDGSWDDLRAARAASDVPLLAKGFFSSEDDLLTAKAAGADAALLI
                     LRDLDDTDASRLMAYASELGLDTLVEAHDAGELDRAVALGAPVIGVNARDLSTFEIDR
                     RAQLELVGSAPRDRIVIAESGIHTRAQGAAAELAGADAILVGSALMQASDPADKLREL
                     LSRPLVKVCGLTRADDVAAAVEAGADMLGFVLVPESPRAAERVLETPEEVLSVAVWVG
                     EAAETDADLDQVHERADEQVRGRDGTLFRGGEPVARVLDLPWDTEDPGHWDRAAAADG
                     RIMLAGRLDADNVREAIERVGPWAVDASSRLESSPGVKDHAKLRAYVEAAHR"
     gene            complement(2161..3180)
                     /gene="trpD"
                     /locus_tag="AC150V_04210"
     CDS             complement(2161..3180)
                     /gene="trpD"
                     /locus_tag="AC150V_04210"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR3762158.1"
                     /translation="MIQEAVAQLLDGSSLDRGEARAVMGEIMAGKATEAQIAGFLVAL
                     RAKGETVDEIAGCAEAMREHVVPVHPSREDLVDTAGTGGDGARTFNISTAAALVAAAA
                     GAGVAKHGNRAVSSASGSADVLEALGFSLEQPAERIARSIDELGFGFMFAPSHHPAMR
                     HAAPVRRELATRTVFNVLGPLTNPAGARAQVIGVYSPELVRTIAEVVKELGARRAFVV
                     HGAHGIDELSPAGPNLVCEVVGGDVHERVIDPRELGIEPCDPDALAGGDAAANAEAIR
                     EIFAGAPGPTREAVLLNAAGAIAAAGDALDLAEGLAQAREAVDSGAAAARLGELVAFS
                     REENS"
     gene            complement(3177..3734)
                     /locus_tag="AC150V_04215"
     CDS             complement(3177..3734)
                     /locus_tag="AC150V_04215"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR3762159.1"
                     /translation="MKVLVIDNYDSFTYNLVHLFEELGAEVVVHRNDAIDPDEAESIA
                     PDRLVVSPGPGRPADAGISVEVIRRLGPTTRTLGVCLGHQAIVEAFGGEVGQARALLH
                     GKASTVRHDGVGVYAGLPDEIQAGRYHSLAAIEVPDALEVTARSVDGEVMGVRHRELG
                     IEGVQFHPESVLTPDGPRMIRNFLS"
     gene            complement(3731..4633)
                     /locus_tag="AC150V_04220"
     CDS             complement(3731..4633)
                     /locus_tag="AC150V_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR3762160.1"
                     /translation="MLAGDPDDVAGRLARETPVPAGGAGTASGELMRFPSRERYEEMV
                     RACKDYIVAGDAFQIVPSQRAERPTSATGLDIYRALRRVNPSPYLFLLELDGISLIGS
                     SPERLVACENGRASLCPIAGTTEPTEGDAERLLSSEKDKAEHVMLVDLGRNDLSRVCR
                     GGTVHVSRYMDVERFSHVSHLVSEVVGELREGMTPFDVLRACFPAGTVSGAPKVRAMQ
                     IISELEGFRRGPYAGAVLYALPGGTMDACIALRTMVLADGVAHLQAGAGIVADSDPAA
                     EHLECLHKLAALEQAIDLAEREYA"
     gene            complement(4713..>5251)
                     /locus_tag="AC150V_04225"
     CDS             complement(4713..>5251)
                     /locus_tag="AC150V_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012163709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3762161.1"
                     /translation="AVFDALTRTAPDDAILCVDVGNNTYSFGRYFECRRQAVLMSGYL
                     GSIGFAYPAAMGAWAATREDDPRFAGRPVVAVSGDGGFGQYMGELTTAVKYGMKITHV
                     LVNNSELGKISKEQRAGNWDVWQTSLHNPDFARYAELCGARGIRVTARGQLDGALAEA
                     FAYEGPSLVEVVADAELV"
BASE COUNT          753 a         1853 c         1863 g          782 t
ORIGIN      
        1 agcgccggga tgatgccctc gagctccgac aaccgacgga agccctcgat ggcgacccgg
       61 tcggtggcgg cgacgtaggt cgcccggccg gtctccgcca gcatcgcgtg ctcggggccg
      121 acacccgggt agtcgagacc ggccgagatg gaatgggcgt ccgagatctg gccgtcctcg
      181 tcggcgagga tcgacgagcg cgagccgtgc agcacgcccg cacgacccgt gccgagcgac
      241 gcggcgcccg ccgcttcgac gccgacgagc tcgactccgt cgtcggccag gaacccggcg
      301 aacatcccga tcgcgttgga gccgccgccg acgctcgcga cgacgacctc cggcagcgtg
      361 cccgcggcct cgaggatctg ggcgcgcgcc tcgcggccga tcacggcctg gagctcccgc
      421 acgagctcgg ggtagggcgc agggccgacg caggagccga tcacgtagtg ggttgtctcg
      481 acgttcgtga tccagtcacg gatcgcctca ctggtcgcct ctttcagtgt gcgtgttccg
      541 aactcgacag gtacgacggt tgcgccgagt aggcccatcc gctcgacgtt gggcgcctga
      601 cgccgcatgt cctcggcgcc catgaagacg acacactgga ggccgaagcg cgcacacgcc
      661 gtggccgtcg cgacgccgtg ctgccccgcg ccggtctcgg cgatgatccg ggtcttgccg
      721 agccgtcgcg caaggacgag ctggccgaga gcgttgttca gcttgtgggc tcccgtgtgg
      781 aggaggtcct ctcgtttcag atacacacgc ttgccgggcg agaagcgctc ggcgagggtg
      841 agaggcgtcg gccgcccggc gtaggtccgg agcagctcgt cgagctcgtc ccagaagctc
      901 cggtcttcct tcgccgactc ccaggcgtcg gtcagctcgt cgagcgcggg aatgagtgtc
      961 tcggggacgt agcgtcctcc gtagcttccg taggtcgtcg tcatcggtgt gccgcctcca
     1021 cgtacgcgcg tagcttggcg tggtctttga cgcccgggga cgactcgagt cgcgagctcg
     1081 cgtcgaccgc ccacggcccg acacgctcga tcgcctcgcg gacgttgtcg gcgtcgagcc
     1141 tgccggcgag catgatgcgc ccgtctgcgg ctgccgcccg atcccagtgt ccaggatctt
     1201 ctgtgtccca gggcagatcg agcaccctgg cgacgggctc gccgccgcgg aacagcgttc
     1261 cgtcacggcc gcgcacctgt tcgtcggcac gttcgtgcac ctggtcgaga tcggcatccg
     1321 tctctgctgc ctctccgacc cagacggcaa ccgagagaac ctcctcgggc gtctcgagca
     1381 cgcgctccgc cgcccgcggg ctctccggga cgagcacgaa gccgagcatg tccgcgccgg
     1441 cctctactgc cgcggcgacg tcgtcggcgc gagtgaggcc gcagaccttc accagtgggc
     1501 gagagaggag ctcacggagc ttgtccgcgg ggtcggaggc ctgcatgaga gccgagccga
     1561 cgaggatggc gtcggctcct gcgagctcgg cggcggctcc ctgtgcccgt gtgtggatgc
     1621 cgctctcagc gatcacgatt cggtcgcggg gcgcgctgcc gacgagctcg agctgtgccc
     1681 gccggtcgat ctcgaacgtg gagaggtcgc gcgcgttgac gccgatcacc ggggcgccga
     1741 gcgcgaccgc ccggtcgagc tcccctgcgt cgtgcgcttc gacgagcgtg tccaggccga
     1801 gctcgctcgc atacgccatc agtcgagacg catcggtgtc gtcgaggtcg cgaaggatga
     1861 gcagcgcggc atcggctccg gccgccttgg cagtaagcag gtcgtcctcg ctcgaaaaga
     1921 accccttggc gagaagaggc acgtcggagg cagcccgcgc cgcccgcagg tcgtcccatg
     1981 agccgtcgaa gcgctcgtcc acgagaaccg acaccgcggc cgcgccggcg tcggcatagg
     2041 cgggcgcgat tccggcagga tcggcgccgg ggcggagatc gccggcggag ggcgagcggc
     2101 gctttatctc ggcgatcgcg ccgagcccag ggcgggccag aatctcctcg aagcggccca
     2161 ctaggagttc tcctctcggc tgaaggcgac cagctcgccg aggcgggccg ccgccgctcc
     2221 cgagtcgacc gcctcccttg cctgtgcgag cccctccgcc aggtcgagtg catctcccgc
     2281 cgcggcgatc gctccggccg cgttcagcag cacggcctcg cgggtcgggc ccggcgcccc
     2341 ggcgaagatc tcccggatcg cctccgcgtt cgcggccgcg tcgccgccgg ccagcgcgtc
     2401 cgggtcgcac ggctcgattc cgagctcgcg cggatcgatg acccgctcgt gcacgtcgcc
     2461 gccgacgacc tcgcacacga ggttcggccc cgcgggcgag agctcgtcga tcccgtgtgc
     2521 gccgtggacg acgaacgcgc gtcgcgctcc gagctccttc acgacctccg caatcgtccg
     2581 gacgagctcg ggcgagtaga cgccgatgac ctgcgcgcgg gcgccggcgg ggttcgtgag
     2641 cggcccgagg acgttgaaga ccgtgcgggt ggcgagctcg cgtcgaaccg gcgctgcgtg
     2701 gcgcatcgcc gggtgatggc tcggcgcgaa catgaagccg aatccgagct cgtcgatcga
     2761 gcgggcgatt cgctcggccg gctgctcgag cgagaagccg agcgcctcga gcacgtcggc
     2821 ggagcccgac gccgaagaga ctgcgcgatt cccgtgtttc gcgactcctg cgccggcggc
     2881 cgcggccacc agtgcggctg cggtcgagat gttgaaggta cgggctccgt cgccgccggt
     2941 gccggcggtg tcgacgagat cctcccggga ggggtgaacc gggacgacgt gctcccgcat
     3001 cgcttcggca cagccggcga tctcgtcgac cgtctcgccc ttcgcgcgca gcgcgacgag
     3061 aaagccggcg atctgagcct cggtcgcctt gccggccatg atctcgccca tcacggctcg
     3121 cgcctcgcca cgatcgagcg atgacccgtc gagcagctgg gcgaccgctt cctggatcac
     3181 gagaggaagt tccggatcat ccgaggtccg tcgggcgtga gcacgctctc gggatggaac
     3241 tggacgcctt cgatgccgag ctcacggtgc cggacgccca tcacttctcc gtccaccgag
     3301 cgggccgtga cctcgagcgc atcaggtacc tcgatcgccg caagggagtg atagcggccg
     3361 gcctggatct cgtcgggcag gcccgcgtag acaccgactc cgtcgtggcg gacggtgctt
     3421 gcctttccgt gtagaagtgc ccgagcctgg ccgacttcgc cgccgaacgc ctctacgatc
     3481 gcctggtgcc cgaggcagac ccccagtgtg cgcgtggtcg ggccgagccg gcggattacc
     3541 tcgaccgaga tgcctgcgtc ggccggccgg cccggcccgg gggagacgac gagccggtcg
     3601 ggggcaatgg actccgcctc gtcgggatcg atcgcgtcgt tgcggtgcac caccacttcg
     3661 gcgcccagct cctcgaacag gtgaacgagg ttgtaggtga aggagtcgta gttgtcgatc
     3721 acgagaacct tcacgcgtac tccctctcgg ccaggtcgat ggcctgctcg agcgcagcca
     3781 gcttgtgcag gcactctagg tgctcggccg cgggatccga gtcggccacg atgcccgcac
     3841 cggcctggag atgggcgacg ccgtccgcca gcaccatcgt gcggagcgcg atgcaggcgt
     3901 ccatcgttcc ccccggcagg gcgtacagga ccgcgcccgc gtacgggccg cgacggaagc
     3961 cctcgagctc cgagatgatc tgcatcgcgc gcaccttcgg agctcccgag accgttcccg
     4021 ccgggaagca cgcgcgcagc acgtcgaagg gcgtcattcc ctcgcgcagc tcgccgacca
     4081 cctccgagac gaggtgcgat acgtgggaga agcgctcgac gtccatgtag cgggagacgt
     4141 ggacggtgcc gccgcggcag acgcgcgaaa ggtcgttgcg tccgaggtcg acgagcatga
     4201 cgtgctctgc cttgtccttc tcggacgaaa gcagtcgctc ggcgtcgcct tccgtcggct
     4261 cggtcgtgcc ggcgatcggg cacaggctgg cgcggccgtt ctcgcaggcg acgaggcgct
     4321 ccggcgacga cccgatcagc gagatcccgt cgagctcgag caggaagagg tacggggagg
     4381 ggttcacgcg gcggagggca cggtagatgt cgagcccggt cgcggaggtg ggccgctcgg
     4441 cccgctgcga ggggacgatc tggaaggcgt cgcccgccac gatgtagtcc ttgcacgccc
     4501 gaaccatctc ctcgtagcgc tcgcggctgg ggaatcgcat tagctcgccg ctcgctgtgc
     4561 ccgctccacc ggcaggcacg ggagtctcgc gcgcgagacg accggcgacg tcgtcgggat
     4621 cgccggcaag cacctcccac tacttcgtcg gcgagatcca cgcctcgccg ccggcggtgc
     4681 gctgaggccg cccgccgccc gccggtgcgg cgtcacacca gctcggcgtc cgcaaccacc
     4741 tcgaccaggc tcggaccttc gtaggcgaac gcctcggcga gcgcgccgtc gagctggccg
     4801 cgcgcggtga cccggatgcc gcgggcgccg cacagctcgg cgtagcgcgc gaagtcgggg
     4861 ttgtggagcg aggtctgcca gacgtcccag ttgccggcgc gctgctcctt cgagatcttg
     4921 cccagctcgg agttgttgac cagcacgtgg gtgatcttca tgccgtactt gacggcggtg
     4981 gtgagctcgc ccatgtactg gccgaagccg ccgtcgcccg acaccgccac caccggccgg
     5041 ccggcgaagc gagggtcgtc ttcccgggtg gccgcccagg cgcccatcgc cgccgggtag
     5101 gcgaaaccga tcgagcccag gtagccggac atcagcaccg cctgccggcg gcactcgaag
     5161 taacggccga acgagtaggt gttgttgccc acgtcgacgc agaggatcgc gtcgtcggga
     5221 gcggtgcggg tcagcgcgtc gaacacggcg a
//
LOCUS       DBNGNK010000148         3130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1815792,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000148 DBNGNK010000000
VERSION     DBNGNK010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1815792"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..156
                     /locus_tag="AC150V_04230"
     tRNA            84..156
                     /locus_tag="AC150V_04230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117..119,aa:Arg,seq:gcg)
     gene            279..587
                     /locus_tag="AC150V_04235"
     CDS             279..587
                     /locus_tag="AC150V_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3762162.1"
                     /translation="MSSTVTTRRPPRRRLRRCRRGPGGLTRRELEVLQVVADGKSNKA
                     VARALWVTDDTVKFHLANIYRRLEVHSRAEAASWARREGLLDDDAEPEFRLVPPRLAA
                     "
     gene            701..1630
                     /locus_tag="AC150V_04240"
     CDS             701..1630
                     /locus_tag="AC150V_04240"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3762163.1"
                     /translation="MPLYLTEDDVESLLTAADAVPIVEECFARIAAGAVEILPRRRLP
                     IPGGYYAVMAATDTELGYAGLKSYTVVDGQLAFVVCLFELERGELAAVIEADRLGQLR
                     TGAASGVAARYLARTGAASVGLIGCGWQAQSQLAAIRAALPTVDRVVAWCRTPERLAD
                     FCRTNGADAGDSASDAGAQDIVVTATTSKDPVLRGDWLREGALVCAIGANDPGARELD
                     NVVLQRASFVCCDSLEQAKVESGDLIEPVVSGVLDWLEVHELQEVVAGETAGRGSERD
                     VVLFKSNGIAAWDVAIGVAAVDRARARGVGREV"
     gene            complement(1642..1923)
                     /locus_tag="AC150V_04245"
     CDS             complement(1642..1923)
                     /locus_tag="AC150V_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762164.1"
                     /translation="MAFKDLFSFLFQRSSGEERVAQYVIREHDRGRSLDEILQDKYVV
                     NRLSEQQQRRLLDRPEIIQAVGGDMVEAARAAVGGEPTGSGAGGSSGAT"
     gene            1948..2286
                     /locus_tag="AC150V_04250"
     CDS             1948..2286
                     /locus_tag="AC150V_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762165.1"
                     /translation="MRRRSAPHFAERRLDGVDDEGNEERIVIWIERRPGAVWTVGRTV
                     NPQFRQSDEPRPDDIVFEGYELADALMSANEALEDDVSVLEDDRREVDVKPFTRKEVL
                     PLLERWFFGR"
     gene            complement(2295..2987)
                     /locus_tag="AC150V_04255"
     CDS             complement(2295..2987)
                     /locus_tag="AC150V_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3762166.1"
                     /translation="MRSVLRVAGVASLIVLVVASVALAARINGTNRADVLRGTAKSDR
                     IDARGGNDRVFGLRGNDVLLGRAGNDRLIGAAGNDTLNGHAGNDRLEGGAGNDTLLGG
                     PGNDTIITGPGKDKVVCGKGIDTVKGDPNDTVAPDCEKVTGAATPKPGDPAALGKNVF
                     ASAGCGGCHTLSDAGTSGAIGPNLDAAKPSKALVVARVTNGRGAMPSFKSRLTNAEIN
                     AVAAYVSSVAGN"
BASE COUNT          480 a         1048 c         1108 g          494 t
ORIGIN      
        1 cggcgcgtca gctggccggc aagtaacagt ctgttacatg cgccggggcg tgtccgggga
       61 cgcggtagac ttcgtcttcc cgcgcgcccg tagctcaggg gatagagcgc tgccctgcgg
      121 aggcagaggt cgcacgttcg aatcgtgccg ggcgcagtcc ttcgtcgaag ctgccgtatg
      181 accggcacgt ccgggatttc ccggcgctcg ctgcgtcgaa aacctgatgc cgaacggttc
      241 ctgccgtgcc accctgggat acgaagaggg aggtcggcat gagctcgact gtgacgacac
      301 ggaggccgcc gcggcgccgc cttcgtcgct gccgccgcgg gccgggtggg ctgacgcggc
      361 gcgagctgga ggtgttgcag gtcgtcgccg acggcaagag caacaaggcg gtggcgcgtg
      421 cgctctgggt gacggacgac accgtcaagt tccacctcgc gaacatctac cgccggctcg
      481 aagtccacag cagggccgag gcggcgagct gggccagacg cgaaggcctc ctcgacgacg
      541 acgcggaacc cgagttcagg ctcgtacccc cccggctcgc cgcgtgaacg gatcggccga
      601 ctcagccgtg tagacgttca ttgcgatcat gggccggagc cgggccgcag atcgacgatg
      661 gctggaacgc aaacggaggg gctactccta gactcctcgc gtgcctctct acctcaccga
      721 ggacgacgtc gagtcgttgc tgaccgccgc cgacgccgtc ccgatcgtgg aagagtgctt
      781 cgcgcgaatc gcggcgggcg ccgtcgagat cctgccgcgg cggagactgc ctatcccggg
      841 cgggtactac gccgtcatgg cggcgaccga cacggagctg gggtatgcgg gcctgaagag
      901 ctacaccgtc gtggacgggc aactcgcctt cgtcgtctgc ctcttcgagc tggaacgagg
      961 agagctcgcc gccgtcatcg aggccgaccg cctaggccag ctccgaacgg gtgccgcgag
     1021 tggagtcgcg gcacgctacc tcgctcgcac cggcgcggcg agcgtcggtc tgatcgggtg
     1081 cggctggcag gcgcagtccc agctggccgc tatccgcgcg gcgttgccca ccgtcgaccg
     1141 cgtggtcgcc tggtgccgaa cgccggaacg ccttgcggac ttctgccgaa cgaacggcgc
     1201 agacgccggc gatagcgcga gcgacgccgg agcgcaggac atcgtcgtca cggcgacgac
     1261 gtcgaaggac ccggttctcc gcggggactg gctgcgcgag ggggccctcg tctgcgccat
     1321 cggcgccaac gatcccggcg cccgtgagct ggacaacgtc gtactacagc gcgcgagctt
     1381 cgtgtgttgc gactcgctgg agcaggcgaa ggtcgagtcg ggcgacctga tcgagccggt
     1441 cgtttctggc gtgctcgact ggctcgaggt acacgagctc caagaggttg tcgccggcga
     1501 gacggcgggg cgcggaagcg agcgtgacgt cgtgctgttc aagtccaacg ggatcgctgc
     1561 ctgggatgtc gcgatcggcg tcgctgcggt cgaccgggcg cgggcgcgcg gcgtcggacg
     1621 ggaggtctga gcgagtccgg gctacgtggc gccgctgctg ccgccggcgc cgctccccgt
     1681 cggctcgccg ccgacagctg cccgagccgc ttcgaccatg tcgcctccga cggcctggat
     1741 tatctccggg cgatcgagca ggcggcgctg ttgttgctcc gacaagcggt tcacgacgta
     1801 cttgtcctgc aggatctcgt cgagcgagcg tccacggtcg tgctcgcgga tcacgtactg
     1861 cgcgacgcgc tcctcaccgc tggagcgctg gaagaggaac gagaagagat ccttgaatgc
     1921 catcggcgca attgtatcct cctggccgtg aggcgccgat ccgcacccca cttcgcagag
     1981 cggcgactcg acggcgtcga cgatgagggc aacgaggagc ggatcgtgat ctggatcgaa
     2041 cgcaggccgg gagcggtctg gacggtcggc cgcacggtca atcctcagtt caggcagtca
     2101 gacgagccac gccccgacga catcgtcttc gagggctacg agttggccga cgcgctcatg
     2161 agcgcaaacg aggcactgga ggacgacgtc tcggtgctcg aggacgaccg gcgcgaggtc
     2221 gacgtcaagc ccttcacgcg gaaggaagtg ctgccgctgc tcgagcgctg gttcttcggg
     2281 cgctgagccg gccgctagtt gcccgcgacc gaagaaacgt acgccgcgac ggcattgatc
     2341 tcggcgttcg tgagccgact cttgaaagac ggcatggcgc cgcgcccgtt cgtgacgcgg
     2401 gccacgacga gggccttcga cggcttcgcc gcgtcgagat tcggtccgat cgcgccgctc
     2461 gtcccggcgt cggaaagcgt gtgacagcca ccacagcccg ccgaggcgaa cacgttcttg
     2521 cccagcgcgg cggggtcgcc gggcttgggt gtggccgcgc ccgtcacctt ctcgcagtcg
     2581 ggggcgaccg tgtcgttcgg atcgcccttc acggtgtcga tgcccttgcc gcagacgacc
     2641 ttgtccttgc cgggtcccgt gatgatggtg tcgtttcccg gcccgccgag cagggtgtcg
     2701 ttgcccgcgc cgccctcgag gcggtcgttt cccgcatgcc cgttcagcgt gtcgttgccg
     2761 gcagcgccga tcagcctgtc gttgccggcc cgtccgagca gcacgtcgtt cccccgcagc
     2821 ccgaacaccc ggtcgttgcc gccccgcgcg tcgatgcggt cagactttgc cgttccgcgc
     2881 aggacatcgg ctcggttcgt gccgttgatc cgggccgcga gcgcgaccga cgcgaccacg
     2941 aggacgatca acgaagcgac tcccgcgacc ctcagaaccg agcgcacaac gagtaccgta
     3001 ggtgatcctg cctcgccagc gcaagggtca gtgaggtcgg atgcgggcgg tacagtgcag
     3061 cacgaccccg tccgaaacgg agcgtcgtag atggccgtac gcgaagcaga gcccgtcggc
     3121 tcgcttcagg
//
LOCUS       DBNGNK010000149        10914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_310669,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000149 DBNGNK010000000
VERSION     DBNGNK010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10914
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_310669"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..955)
                     /locus_tag="AC150V_04260"
     CDS             complement(<1..955)
                     /locus_tag="AC150V_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3762167.1"
                     /translation="MRLLLVPLAAVLSLAVLTAAPAPGATVAKKKPTCVKPAGLTFTR
                     KLGRSDGLLRWKRPRRLPADAAGYRVLRDGKVVGQTRRLRMRVKVKLGKRVRLAVKVA
                     LKDGRALRCATTVTRRLPWKLPGPASNLVARPTDAGVVLRWERARRGEGKLVGYRVFR
                     DGKPMQQVRGRTIAVPLPSLRTFTFAVAAVDTRGKLGRRSNVVRVRMGHEPPSAPPSI
                     FGEPINDREVQLRWEPSSGTRGVRVFYRVLRDGVTITQTPMPEARVANLAPGTSYSFS
                     VLAVDSLGYASEATAQVTVTTKPPEQSSGGAHVFLLASTDAS"
     gene            complement(952..2673)
                     /locus_tag="AC150V_04265"
     CDS             complement(952..2673)
                     /locus_tag="AC150V_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3762168.1"
                     /translation="MRGVWRRSSDRPRWRVEAVVGLLMLPLTLIPIYLYMTRTDDGYL
                     MYLHGRYAVMPPGTKELGTETAQLARDAVREPIHGVPVLVYHGIGRNTGDVDDRRYLV
                     TRDHFAEHMRALAAAGFQAITTGELALYLRSRDPNLLPRKPVLITFDDGRTDAMVQAD
                     PILGDTGMKATMFVIGRAASEPSFYYQDWGDLRGHVASGRWELGNHTAELHRAHDDVR
                     GRPPVSALARPEPGESIEAFEARIAADLDRAQDLLREKGSGNPIAFSYPFGDWGQQAR
                     VPGVPAAIERVLADRFALAFDQDHQSGWRFALPGDDPLHIHRLQMQNWTGAEFLERLA
                     AAEELSETAFRERGLDLGYSPDRLFAAAVQQACARPRPTPIRATPAAGMKAVALTFDD
                     GPSAYTPQVLDVLGQYGAKATFFVVGRRLQGRDTVLERMLVEGHEIANHTWSHRHAQD
                     ISSDALSRELRATSARIAAAVPTKPCLTRPPYNEGVRRHAGVGLSLGMSTALWSVDPR
                     DFELRSGWRIANRVLKQVDGGDIVLLHDGGSDRWATVQALPLILEGLDRRGLSAVTVS
                     ELVGSGG"
     gene            complement(2670..4055)
                     /locus_tag="AC150V_04270"
     CDS             complement(2670..4055)
                     /locus_tag="AC150V_04270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3762169.1"
                     /translation="MAPFRPFDESTAALSPELHERRGVRLVVLVLQVTCILYAAFLLY
                     WVWLAKDADVALIFRDPVFYTYSLLVTGYVVARFLLAPFYKPARDAGYRPSVSVVIPA
                     FNEQDAIEATIDAIYSSDYPREKLEVVVVDDGSDDATWERILAARERNPSVVCVRFPD
                     NRGKRAAMAEGIRRSGAEVCVFIDSDSVIEPDGLARIVADFVDPEVGAVVGHADVLNK
                     TANWLTRMQQVRYFVAFRVIKGSESLFGAVTCASGCFSAYRRTALLAILDRWESQRFL
                     GRPATFGDDRALTNYVLRDWRVTYQSEARSHTIVPEGVRVFARQQLRWKKSWLRESLH
                     VSRFIWRKNPVAAVLTYLGCIFPWIAPLVVVHAVWFRAVGVGSPLFYLVGAYVMAVLY
                     SLYYAISRRSPLWWHGITFVAVYMAFLVWQTYYALLTIRNTKWGTRSSTGGDTGGEIT
                     VLKPATRGEAQ"
     gene            complement(4263..4793)
                     /locus_tag="AC150V_04275"
     CDS             complement(4263..4793)
                     /locus_tag="AC150V_04275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3762170.1"
                     /translation="MNVGLRRATPDDLDFLVGLLTDEETAPFLAAVRPQGRDEIAALV
                     ERSTREPETFGLFVIEVDDERAGTVEFELVNRRSSIANLGGLALAQPFRGRGLGTAAA
                     RELQRHLFFDLGLHRLQLEIYAFNERAIAHAERSGFIREGVRRRAYRRRGTWVDGVLF
                     GLVREDLDEQGPSTPT"
     gene            complement(4790..5713)
                     /locus_tag="AC150V_04280"
     CDS             complement(4790..5713)
                     /locus_tag="AC150V_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3762171.1"
                     /translation="MTRGYWPLILVLAAAWGASFLFIKLAVDDIEPAPMMAARSLLAG
                     AVLIPAVLLRAGRPALSELRSSWRDAVVLGAINGAIPFWLLGWSEKHIDSSVAGVAMA
                     AVPLFAFLLGLRFLPHERITGLRWAGVALGLVGVAALVGFEPSGGWWAVAGGLAAVGA
                     AGCYASGGTYAQLRVRAVSGPVLATGSMLAAGVFLLPVALFQLPDTTPGGRSLLGLLG
                     LTIVGTAFAQLLFFRAVRLFGMRRVSLIAYLMPCFAVFYGALLLDEPLTAGMLGGLVL
                     ILLGVALGSGAVGARRRTTDQGNGEVAAPAP"
     gene            complement(5723..6583)
                     /locus_tag="AC150V_04285"
     CDS             complement(5723..6583)
                     /locus_tag="AC150V_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3762172.1"
                     /translation="MTRRDAFYLLLLSAIWGSSFTFIKLGVDEMTPAVVVLGRLVVGV
                     AVLLPLTAGRGGLSPIRAFLVPLSVLGLLNNALPFWLLGFAEERIDSGLAAVIQAVAP
                     IVTVLLAWRLDPGQRVQGLRLVGIAVGFAGVVVLVGDQRGGELVGALAVLGTATCYAV
                     SVLYAGRTLRGLTPLHASTGQLAAATLVIAPFALVELPGSTPSAKAIAAVVALGALGT
                     GAAYLLYFTIIRNAGASRAILITYLVPAFALLFGIVFLDEPVAAAALGGLALILLGTA
                     LATGLLRRRR"
     gene            6658..9105
                     /locus_tag="AC150V_04290"
     CDS             6658..9105
                     /locus_tag="AC150V_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR3762173.1"
                     /translation="MATGEQVPGDAYSLPASRVAAIIAADTDVGLTEVEAQRRLVEHG
                     PNALAAPGRPPYVRIAARQFVNPLVALLVAAAVVSASIGEGLEAAVIASIVVLNAVLG
                     FSLEAGAEREVLALHASLQLEVSVVRDGVESVIPAEAVVPGDLLRVREGDRVGADARV
                     LSTQGLEVDESLLTGESAPVGKSPDPVPAGTTLAEHFSMVFSGTAVTRGSAYVLVTAT
                     GRHTEQGKIAGLVERAAPPPTPLQTRLNRLAQVLVVGGVLITLALAAIMLLRDEPGRQ
                     AFLVGVSVAVAAVPEGLAAILTIALALGSRTMSRRGAIVRSLGAIETVGESTSICADK
                     TGTLTQNRMRLERIEPAAQRSEDDVLLAARAASAAEVDPVDRALVLAAADRGIDAEIE
                     LIRSLPFEAARKRATVVVAVDGGLRSIVKGAPEVVLARSRAEKAEDAERLLGVAEEWA
                     AEGVRVLAVGSRRVTDPERDPLEDDLEPLGLVGLEDPLRENAAESVARARDLGLSVKM
                     LTGDHALTAAAIGKRLGLAADDINSRFTPANKLELVERLQRQGEVVAVTGDGVNDAPA
                     LRQADVGIAMGESGTEAAREASSLVLTDDDFATIVAAVEEGRRVGANVRSVLAHFLSG
                     NVGEVVLFVVAIGAGLGVPMTVVQVLVVNLLTDGSPAIALAADRATRGRVWLARGAPL
                     LGRGVVTGLLGVALVIGLVSLVAFVVLRETRPEAAQTAAFATLALAELGFAFSCRSDT
                     QPPWRLKANRWLWLAVGVSLSIVAVLLYIPALHEPFGTVALTVEELILVLALAVVPFV
                     LAETLKAVARRHSRGRG"
     gene            9124..>10914
                     /gene="purL"
                     /locus_tag="AC150V_04295"
     CDS             9124..>10914
                     /gene="purL"
                     /locus_tag="AC150V_04295"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR3762174.1"
                     /translation="MAEQPLHRQLGLTDGELDRMCSLLEREPNDFELAVFSLLWSEHC
                     GYKHSAPLLRRLPSDGERVLQGPGENAGVLDLGDGLAVAFKVESHNHPSAVEPFQGAA
                     TGVGGILRDIAAMGARPVALLDGLRFGAPDWHFGRAVGGIGHYGNCVGVPTVGGEAVF
                     DDAYRDNCLVNAMCVGLLPTESLTRAKATASGSLVVLYGATTGRDGIGGASVLASQEL
                     GDGAHEKRPSVQIGDPFTGKRLIEVSVELAERGLVESLQDCGAAGLASSLSEMAAGYG
                     IDVHLDRVPVREDGMEPWEVMISESQERMVAIVNHDRLAEVQSVCDRWELPHAVIGEV
                     TDSGELRAFWDGESVGAIPARFLTEECPRYEVERRPRSAGGEVPVTEAPPAGEALLAL
                     LRSPNLRSRAFVYRRYDQLVQSRTVRRPGFDAAVLRLRPSYRGLALSLDGQGRLAAAD
                     PFTGGARAILEAARNVAAVGGEPLGFTDCLNFGNPEKPEVGWELSEVIEGMARACEAL
                     ELPVVSGNVSLYNDTDGVSIHPTPVVGCVGLVTDVRRVPDAWREGDVILLATAGSISL
                     AGSEYQALFGETSGQPPELDLRAEAALVRHL"
BASE COUNT         1669 a         3737 c         3916 g         1592 t
ORIGIN      
        1 agctggcgtc ggtcgacgcg aggaggaaga cgtgcgcccc gccgctcgac tgctccggcg
       61 gcttcgtcgt gacggtgacc tgggcggtgg cctcgctcgc gtagcccagc gagtcgacgg
      121 caagaaccga gaaggagtag ctcgtgccgg gcgcgaggtt cgcgacccgt gcctccggca
      181 tcggcgtctg cgtgatcgtg acgccgtcgc gaagaacccg gtagaaaacg cggactcctc
      241 tcgttccgga gctcggctcc cagcggagct gtacctcgcg gtcgttgatc ggctcgccga
      301 agatgctcgg cggcgcgctg ggaggctcgt ggcccatccg tacgcggacg acgttcgagc
      361 gccggcccag cttcccccgt gtgtccaccg cggcgaccgc gaaggtgaac gtccggagcg
      421 aggggagcgg caccgcgatc gtacgacccc gaacttgctg catgggcttg ccgtcgcgga
      481 agacgcggta gccgaccagc ttgccctcgc cgcggcgggc cctttcccac cgcaggacga
      541 cacccgcgtc cgttggtctc gcgacgagat tcgacgcagg tccgggtagc ttccagggga
      601 ggcggcgcgt gaccgtcgtc gcgcaccgga gcgcccggcc gtccttcagc gcgaccttca
      661 ccgccagccg cactcgcttg ccgagcttca ccttcacgcg catgcgcagc cgccgtgtct
      721 gccccacgac cttcccgtcg cgcaggacgc ggtaccccgc ggcgtcggcg ggtagccggc
      781 ggggccgctt ccagcgcagc agcccgtcgc tgcgcccgag cttccgcgtg aacgtcagtc
      841 ccgccggctt gacacacgtc ggcttcttct tggcgacggt ggcgcccggg gccggggcgg
      901 cagtgagcac ggcgagcgag agcactgcgg caagcggaac gagcaggagc ctcatccgcc
      961 gctcccgacc agctcggaca cggtgaccgc cgacagaccg cgcctgtcga gtccctcgag
     1021 gatgagcggt agcgcctgaa ccgtcgccca gcggtcgctg ccgccgtcgt ggagcaggac
     1081 gatgtcgccg ccgtcgacct gtttcaggac gcggttggcg atccgccacc ccgagcgcag
     1141 ctcgaagtcc ctcgggtcga ccgaccacag cgccgtcgac atgcccagcg agagcccgac
     1201 gccggcgtgg cgtctgacgc cctcgttgta cggcggccgc gtcaggcacg gcttcgtcgg
     1261 aaccgccgcg gcgatcctgg cgctcgtggc acgaagctcg cggctgagcg cgtcactgct
     1321 gatgtcctgc gcgtgccggt gcgaccacgt gtggttcgcg atctcgtggc cctcgaccag
     1381 catgcgctcg agcacggtgt cccgcccctg cagacgccgg ccgacgacga agaatgtcgc
     1441 cttcgccccg tactgaccca acacgtcgag cacctgcggc gtgtaggccg acgggccgtc
     1501 gtcgaacgtc agggcaactg ccttcatccc cgctgccgga gtcgcccgga tcggcgtcgg
     1561 ccgaggccga gcacaagcct gctggacggc cgctgcgaag agccggtcgg gtgagtagcc
     1621 gaggtcgagc ccgcgctcgc ggaacgccgt ctcgctcagc tcctctgctg cggccagccg
     1681 ctcgaggaac tcggcgccgg tccagttctg catctgcagg cggtgtatgt gcagcgggtc
     1741 gtcgccgggg agcgcgaagc gccagccgga ctggtggtct tgatcgaagg cgagagcaaa
     1801 gcgatccgcg aggacacgct cgattgcagc cgggacgccc ggaactctgg cctgctgccc
     1861 ccagtcgccg aatggatagg aaaaggcgat cgggttcccc gatcctttct cccgcagcag
     1921 gtcctgtgcg cggtcgaggt cggcagcgat gcgcgcttcg aacgcctcga tcgactcgcc
     1981 gggctcaggc cgcgcgagtg ccgagacggg tggacgtccg cgcacgtcgt cgtgagcacg
     2041 atgcagttcg gcggtgtggt tcccgagctc ccaccggccg ctcgcgacgt gtccgcggag
     2101 gtcgccccag tcctggtagt agaagctggg ctcggacgca gcgcggccga tcacgaacat
     2161 cgttgccttc atgcccgtgt ctcccaggat cgggtcggcc tgcaccattg cgtcggtccg
     2221 gccgtcgtcg aacgtgatca gcacgggctt gcgcgggagc aggttcgggt cgcggctacg
     2281 gaggtacagc gcgagctcgc cggtggtgat cgcctggaac ccggcggcgg cgagtgcgcg
     2341 catgtgctcg gcgaagtggt cccgtgtgac gagatagcgg cggtcgtcga cgtcgcccgt
     2401 gtttcgtccg atcccgtggt agacgagcac cggaacgccg tgaatcggct cccgaaccgc
     2461 atcgcgggcc agctgcgcgg tctccgtgcc gagctccttc gtgcctggcg gcatgaccgc
     2521 gtagcgtccg tgcaggtaca tcaggtaccc gtcatcggtt cgggtcatgt agaggtagat
     2581 cgggatcagc gtcagtggca gcatgagcag cccgaccacg gcctcgaccc gccagcgcgg
     2641 ccggtcggac gagcggcgcc agacgcctct cattgcgcct cgcctcgggt cgcgggcttc
     2701 agcaccgtga tctcccctcc ggtatcgccg cctgtcgagg agcgcgtgcc ccactttgtg
     2761 tttcggatcg tgaggagcgc gtagtacgtc tgccagacga gaaaggccat gtagacggcg
     2821 acgaaggtga ttccgtgcca ccagagcggg ctccggcggc tgatcgcgta gtagagggag
     2881 tagaggacgg ccatcacgta ggcgccgacg aggtagaaga gcgggctgcc gacgccgacg
     2941 gcccgaaacc acaccgcgtg aacgacgacc agaggcgcga tccacgggaa gatgcagccc
     3001 aggtacgtga gaacggcggc taccgggttc ttcctccaga tgaagcgcga gacgtgcagc
     3061 gactcccgca gccacgactt cttccagcgc agctgctggc gggcgaagac gcggacgccc
     3121 tccgggacga tcgtgtgcga gcgcgcctcc gactggtagg tgacgcgcca gtcgcgcagg
     3181 acgtagttgg tgagggcgcg gtcgtccccg aatgtcgccg gccggccgag aaagcgctgg
     3241 ctctcccacc ggtcgagaat cgccagcagc gccgtgcgcc ggtaggcgct gaagcagccc
     3301 gacgcgcacg tgaccgcgcc gaacagcgac tcgctcccct tgatcacccg gaaggcgacg
     3361 aagtaacgca cttgctgcat gcgtgtgagc caattcgccg tcttgttgag gacgtcggcg
     3421 tggcccacga ccgctccgac ctccggatcc acgaagtcgg cgacgattcg tgccaacccg
     3481 tccggctcga tcaccgagtc ggagtcgatg aagacgcaga cctcggcacc cgagcggcgg
     3541 atcccctcgg ccatcgccgc ccgcttcccc cggttgtcgg ggaagcggac gcagacgacg
     3601 gacgggttcc gctcgcgggc ggcgaggatc cgctcccagg tcgcgtcgtc ggacccgtcg
     3661 tcgacgacga ccacctccag cttctcccgc ggatagtcgc tcgagtagat ggcgtcgatc
     3721 gtggcctcga tcgcatcctg ctcgttgaag gcggggatga cgaccgagac gctcgggcgg
     3781 tagccggcgt cccgagccgg cttgtagaag ggggcaagca gaaatcgggc gaccacgtag
     3841 ccagtgacca gcagtgagta cgtgtagaag acggggtcgc ggaagatcag cgcgacgtcg
     3901 gcatccttcg caagccacac ccagtagagg aggaaggctg cgtagaggat gcaggtgacc
     3961 tggaggacga ggacgaccag ccgcactccg cgacgctcgt gcagttcggg tgacagcgcc
     4021 gcagtcgact cgtcgaaggg ccggaacgga gccattctcg gccgcggatc ccgctcaggc
     4081 gtcgacggcg gctccgcgag ccggaggccg gtcttcgaat agcgccccgt ggtggtccgt
     4141 tcctgtgccg caggatcggc aggcgtcaca ccccggggag gcttggcggc accctcccag
     4201 tcacggtccc acgatcggcg ctgcggcacc cgacgcgaag ccctcgaacg aggggtatct
     4261 ggttacgttg gggtgctcgg gccctgctcg tcgaggtctt cgcgcacgag accgaagagc
     4321 acgccgtcga cccacgtgcc gcgtcggcgg tatgccctcc ggcgcacgcc ctcgcggatg
     4381 aagccggacc tctccgcgtg ggcgatcgcg cgctcgttga aggcgtagat ctccagctgg
     4441 agccggtgca gcccgagatc gaagaagagg tggcgctgga gctcgcgtgc cgctgcggtg
     4501 ccgaggcccc gacctcggaa gggctgagcg agtgcgagcc cgccgaggtt ggcaatcgag
     4561 ctccgccggt tgaccagctc gaactcgacg gtgccggccc gctcgtcgtc cacttcgatc
     4621 acgaacaacc cgaaggtctc cggctcccgc gtcgagcgct cgacgagcgc agcgatctcg
     4681 tctcgaccct gcggccgaac cgccgccagg aagggcgcgg tctcctcgtc ggtgaggagc
     4741 ccgacgagga agtcgaggtc gtccggggtc gccctgcgaa gaccgacgtt cacggcgcgg
     4801 gtgcagcgac ttcgccgttg ccttggtcgg tcgtccgacg gcgggctcct accgcgccag
     4861 agcccagcgc caccccgagg agaatgagga cgaggccgcc cagcattccc gccgtcagcg
     4921 gctcgtcgag caggagcgca ccatagaaga ccgcgaagca cggcatcaga tacgcgatca
     4981 gcgagaccct tcgcatgccg aacagtcgaa cggcccggaa gaagaggagc tgggcgaacg
     5041 cggtcccgac gatcgtcagt ccgagcagtc cgagcagcga gcgcccaccg ggggtcgtat
     5101 cgggcagctg gaacaaggcg acggggagca ggaagacccc ggccgccagc atggagccgg
     5161 tcgcgagcac cggaccgctg acggcccgga cccggagctg cgcgtaggtg ccgccggacg
     5221 cgtagcagcc cgccgcgccg actgccgcga gccctcccgc gaccgcccac cagccgccgc
     5281 tcggctcgaa gccgacgagt gcggccactc cgacgaggcc gagcgcgaca ccggcccagc
     5341 gcaggcccgt gatccgctcg tgggggagaa agcggagccc gagcaggaaa gcgaacagcg
     5401 ggacggccgc catcgcgaca cccgcgacgc tcgagtcgat gtgcttctcg ctccagccga
     5461 ggagccagaa cgggatcgcc ccgttgatcg cgccgagcac gaccgcgtcc cgccatgacg
     5521 agcgcagctc cgagagagcc ggccgacccg cccgcaacag gacggcaggg atcagaacgg
     5581 cgccggcgag cagagagcgg gccgccatca tcggtgccgg ctcgatgtcg tcgactgcga
     5641 gcttgatgaa gaggaacgac gccccccaag cggccgccaa cacgaggatc agaggccagt
     5701 agccgcgcgt cacgatgcga ggctacctcc ggcgccgcag cagccccgtt gcgagtgccg
     5761 ttcccagcag gatgagggcc agccctccca gcgccgccgc agcaaccggc tcgtcgagga
     5821 agacgatgcc gaagaggagc gcgaacgcgg gcacgaggta ggtgatcagg atcgcgcgcg
     5881 atgcgcctgc attgcgaatg atcgtgaagt agaggaggta ggcggcaccc gttcccagcg
     5941 cgccgagtgc gaccaccgct gcgatcgcct tcgcgctcgg cgtcgagccc ggcagttcga
     6001 ccagggcgaa gggggcgatg accagggtcg ccgccgcaag ttgtccggtc gaggcgtgga
     6061 gcggcgtcag cccgcgcagt gtcctgcccg cgtagaggac ggagaccgcg tagcaggttg
     6121 ccgttccgag caccgccagg gcgccgacga gctcgccgcc acgctggtcg ccgacgagca
     6181 ccacgacgcc ggcgaagccg acagcgatcc ccaccaggcg caggccctgg acacgctggc
     6241 ccgggtcaag gcgccaggcc agcagcaccg tgacgatcgg cgccaccgcc tggatgacgg
     6301 cggcgaggcc ggagtcgatc cgttcctcgg caaagccgag aagccagaag gggagagcgt
     6361 tgttgagcag gcccagaacg gagaggggga ccaggaacgc acgaatcggg gaaagcccgc
     6421 cgcggccggc cgtcaggggc aacagcactg cgaccccgac gactagccgc cccagcacca
     6481 cgaccgccgg tgtcatctcg tccacgccga gcttgatgaa ggtgaaggac gagccccaga
     6541 tggcggacag gagcagcagg tagaaggcgt cgcggcgggt cacgggcgtc taccgacgct
     6601 agacggttgt cactaccgag gcgcgcgccg gcgtcagcgg ccattcttca ggtacggatg
     6661 gcgactggag agcaggtacc gggcgacgcg tactcgctgc ctgcgagccg cgtcgccgcc
     6721 atcattgccg ccgacacaga cgtcgggctg acagaggtcg aggcgcagcg gcgcctcgtc
     6781 gagcacggac cgaatgccct tgcggcgccc ggccggccgc cgtacgtccg tatcgcggcg
     6841 cgtcagttcg tgaatccgct cgtcgcgctg ctcgtcgcgg ccgcggtcgt gtcggcctcg
     6901 atcggcgaag ggctcgaggc cgcagtcatc gcttcgatcg tcgtcctcaa cgcggtgctc
     6961 ggcttttcgc tcgaggccgg cgccgagcgc gaggtgcttg ccttgcacgc gtcgctgcag
     7021 ctcgaggtgt ccgtcgtacg tgacggagtc gagagcgtga tcccggccga ggccgtcgta
     7081 ccgggagacc tgcttcgcgt ccgcgagggc gaccgggtgg gcgcggacgc gcgcgttctc
     7141 tccacgcaag ggctcgaggt cgacgagtcg ctgctcaccg gcgagtcggc ccccgtcggg
     7201 aagtcgccgg atcccgtgcc cgccgggacg accctggccg agcatttctc gatggtcttc
     7261 tccggcaccg ccgtcacacg tgggagcgcc tacgtgctgg tgaccgcgac cggccggcac
     7321 accgagcagg ggaagatcgc gggcctggtc gagcgtgccg ctccgccccc gacgccgctc
     7381 cagacgcgct tgaaccggct ggcgcaggtg ctcgtcgtgg gaggcgtgct gattactctg
     7441 gcgctcgcag caatcatgct cctccgcgac gagccggggc gtcaggcatt tctcgtcggc
     7501 gtctcagtcg ccgtcgcggc cgtgcccgag ggactggcgg cgatcctcac cattgcgctg
     7561 gcgctcggct cacgcacgat gtcgagacgt ggcgcaatcg tacggtcgct gggcgcgatc
     7621 gagacggtag gggagtccac gtcgatctgt gcggacaaga ccgggacgct gacgcagaac
     7681 cggatgcgcc tcgagcgtat cgagccggcg gcgcaaagaa gcgaggacga cgtgcttctg
     7741 gcggcgcggg ccgcctcggc ggccgaggta gatcccgtcg atcgtgcgct tgtgctggcg
     7801 gcggcggacc gaggcatcga tgcagagatc gagctgatcc gctcgctccc cttcgaggcg
     7861 gcacggaagc gggctaccgt cgtcgtcgcg gtcgacggcg gcctgcgctc gatcgtcaag
     7921 ggcgctccgg aggtcgtgct cgcgcgcagt cgtgcagaga aagcggagga cgctgagcgc
     7981 ctgctcggag tcgccgagga gtgggccgcg gagggcgtcc gggtactcgc ggtcgggtcg
     8041 cggcgcgtca ccgatcccga gcgcgacccg ctcgaggacg atctggagcc gctcgggctg
     8101 gtcgggctcg aggaccctct gcgcgagaac gccgccgagt cggtcgcgcg ggctcgtgac
     8161 ctcggcctca gcgtgaagat gctgacgggc gatcacgcgc ttacggcggc ggcgatcggc
     8221 aagcggctag gtttggcggc cgacgacatc aactcccgct tcacccccgc gaacaagctc
     8281 gagctggtgg agcggctcca acgccagggc gaggtcgtcg cagtcacggg tgacggcgtc
     8341 aacgacgctc cggccctacg ccaggccgac gtcggcatcg cgatgggcga gtccggcacc
     8401 gaggcagccc gggaggcgtc ctcgctcgtc ctcaccgacg acgacttcgc gacgatcgtg
     8461 gccgcggtcg aggaggggcg tcgcgtcggc gccaacgtgc gatccgtcct cgcgcacttc
     8521 ctctccggca acgtcggcga agtggtgctc ttcgtcgtcg cgatcggcgc cgggttgggg
     8581 gtgccgatga ccgtggttca ggtgttggtc gtgaatctct tgacagacgg gtctccggcg
     8641 atcgcgcttg ccgctgatcg tgcgacgcga ggacgggtct ggctcgctcg cggcgctccc
     8701 ctgctcggtc gcggcgtcgt gaccgggctg cttggggtcg ctctcgtgat cgggctggtc
     8761 tcactcgtgg cgttcgtagt tctgagggag acccgtcccg aggccgcgca gaccgcggcg
     8821 ttcgcgacgc tcgcgctggc agagctgggc tttgccttct catgtcgctc ggacacgcag
     8881 ccgccgtggc ggctcaaggc gaacaggtgg ctatggctgg cggtaggcgt ctcgttgtcc
     8941 atcgtcgcgg ttcttctcta cattcccgca ctgcacgagc cgttcggcac ggtcgccctg
     9001 acagtggagg agctcatctt ggttctcgcc ctggccgtcg tgcctttcgt gctcgccgag
     9061 acgttgaagg cggtggcgcg gaggcacagc agggggcgcg gataaactga gcttctgagc
     9121 tcgatcgcgg aacaaccgct ccaccgccag ctcggcctca cggacgggga gctcgatcga
     9181 atgtgctcgt tgctcgagcg cgaaccgaac gacttcgagc tcgccgtctt ctcgctcctg
     9241 tggtcggagc actgcgggta caagcactcg gcgccgctcc tgcgccgact cccgagcgat
     9301 ggcgagcggg tcctccaggg gcccggtgag aacgccgggg tgctcgacct cggcgacgga
     9361 ctcgccgtcg ccttcaaggt cgagagccac aatcacccct ccgccgtcga gccgttccag
     9421 ggcgcggcga cgggagtggg cgggatcctg cgcgacatcg ccgcgatggg ggcgcgaccc
     9481 gttgcgctgc tggacgggct ccgcttcggc gcccccgact ggcacttcgg ccgcgccgtc
     9541 ggcggaatcg gacactacgg gaactgcgtc ggggtgccga cggtcggcgg cgaggccgtc
     9601 ttcgacgacg cctaccgcga caactgcctc gtcaacgcga tgtgcgtcgg tctgcttccg
     9661 accgagagcc tgacgcgggc gaaggcgacg gcctccggca gtctcgtcgt cctctacggg
     9721 gctacgaccg ggcgcgacgg catcggcgga gcgtccgtgc tcgcgagcca ggagctaggc
     9781 gacggagcgc acgagaagcg tccttccgtg cagatcggcg atcccttcac cggcaagcgg
     9841 ctgatcgagg tgtcggtcga gctcgccgag cggggcctcg tcgagtcgtt gcaggactgc
     9901 ggagccgccg ggctcgcctc atcgctctcg gagatggcgg cgggctacgg gatcgacgtg
     9961 catctcgacc gggtgcccgt ccgcgaggac ggaatggagc cctgggaggt gatgatctcc
    10021 gagtcgcagg agcggatggt cgcgatcgtc aatcacgacc gactcgccga ggtgcagtcc
    10081 gtctgcgacc ggtgggagct gcctcatgca gtgatcgggg aggtgacgga cagcggcgag
    10141 ctgcgcgcgt tctgggacgg agagtcggtc ggcgccatcc cggcgcgctt cctcaccgag
    10201 gagtgccctc gctacgaggt cgagcgccgg ccgcgttccg cgggcggcga ggtgcccgtg
    10261 acggaggcgc cgcccgccgg cgaggcgctg cttgccctcc tccgctcccc caatcttcgc
    10321 tcccgcgcgt tcgtgtaccg ccgctacgac cagctggtgc agtctcgaac ggtgcgccgc
    10381 cccggcttcg acgccgccgt gctgcggctg cgaccctcgt atcgggggct cgcgttgagc
    10441 ctggacggcc aggggcggct cgccgccgcc gatccgttca ctggcggcgc ccgcgcgatc
    10501 ctcgaggcgg cacggaacgt cgccgccgtc gggggcgagc cgctcgggtt caccgactgc
    10561 ctcaacttcg gcaatccgga gaagcccgag gtcggctggg agctctccga ggtgatcgag
    10621 ggcatggcgc gggcgtgcga ggcgctcgag ctgccggtcg tctcgggcaa cgtctccctg
    10681 tacaacgaca ccgacggcgt gtcgatccat cccacaccgg tcgtcggctg cgtcggcctg
    10741 gtgaccgacg tgcgacgcgt ccccgacgcc tggcgcgagg gcgacgtcat tctcctggcc
    10801 acggccgggt cgatctcgct cgcgggctcc gagtaccaag ccctcttcgg cgagacgtcc
    10861 gggcagccgc cggagctcga ccttcgtgcg gaggcggcgc tcgttcgcca tctg
//
LOCUS       DBNGNK010000150         2515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1385899,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000150 DBNGNK010000000
VERSION     DBNGNK010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1385899"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="AC150V_04300"
     CDS             <1..343
                     /locus_tag="AC150V_04300"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3762175.1"
                     /translation="VGGAGTGGLDADAIAGAEIVGDALRGLDRATELVGLGGGRDLAG
                     YLSVVGRRIKRRRRDADLSQRELSERSGLTRPYLSAVERGKQNITLEAALKIAAVLDV
                     PMTELLDEGWS"
     gene            345..1838
                     /locus_tag="AC150V_04305"
     CDS             345..1838
                     /locus_tag="AC150V_04305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006382187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3762176.1"
                     /translation="MRRILVTGATGYVGGRLVPILLERGFAVRCLARDARKVADRPWS
                     GHPSLEVVEGDASVDADLDRALAGCEAAYYLLHSMVVAGTEYAGRDRRMAERFAAIAE
                     AQHVGRIIYLGGLGETGDDLSEHLASRREVEEALASTRVPVTVLRAAMIIGSGSASFE
                     MLRYLVERLPVMVTPRWVRTECQPIAIRDALHYLVTSLETPETTGRTLDIGGRDVVTY
                     RELMDETAAALGLSERLVLPLPVLTPGLSSLWIHLVTPLSHRIARPLTEGLRNRVVCR
                     DDAAARLMPTELLSAAEAIRLALRASRQPNVATSWSDAGPVPGDPEWAGGTAFRDERQ
                     AAVDAGPGTAFRAIRRIGGGNGWYAADLLWRIRGSLDRLVGGPGLRRGRRDPEVLRHG
                     DALDFWRVVEVEPDRRLRLRAEMKLPGVAYLEFDVEPVGSGSRIVQTAVFHPRGLFGL
                     LYWYAVLPFHGFVFSGMLRGIVRTAEEDGASTAADRGPASPASPASR"
     gene            complement(1906..2340)
                     /locus_tag="AC150V_04310"
     CDS             complement(1906..2340)
                     /locus_tag="AC150V_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762177.1"
                     /translation="MGDAALLGQLATTLPLVGLILVVQIVHYPLFTAVGEHGFAAYHR
                     RHSHRIGLLVVPLMLGEAAAATAWLVSPPAWLGPGIALSGAALLAVVWLSTALLQVPR
                     HARLSHGYDDRTVRTLVASNWLRTAAWTARGVLLLSVVAARI"
BASE COUNT          351 a          855 c          944 g          365 t
ORIGIN      
        1 cgtcggaggg gccgggacgg gtgggctcga cgccgatgcg atcgccgggg cggagatcgt
       61 cggggacgcc ctgcgaggtc tcgaccgcgc cacggagctc gttgggctgg ggggcggccg
      121 ggatctcgcc ggctacctct ccgtcgtcgg tcgtcggatc aagcggcgtc gccgggatgc
      181 cgacctgagt cagcgcgagt tgtcggagcg ttccgggctc acccgtccct acctgtcagc
      241 cgtcgagcgc ggtaagcaga acatcacgct cgaagcggcg ctgaagatcg ccgcggtgct
      301 ggacgtgccg atgaccgagc tgctcgacga agggtggagc tgacgtgcgg cggatcctcg
      361 tcactggcgc cacgggctac gtcgggggtc gcctggtccc gattcttctc gagcggggct
      421 tcgcggtgcg ctgcctcgcc cgcgacgcga ggaaggtggc cgaccggccc tggtccgggc
      481 acccatcgct cgaggtggtc gagggtgacg ccagcgtcga cgccgatctc gaccgggcgc
      541 tcgccggatg cgaggcagcc tactacctgc ttcactcgat ggtcgtggcc ggcaccgagt
      601 acgccgggcg cgaccggcgc atggccgaac ggtttgcggc gatcgccgag gcgcagcacg
      661 tcgggcggat catctacctc ggagggctcg gtgagaccgg tgacgatctg agcgagcacc
      721 tcgcgtcccg tcgcgaggtc gaggaggcgc tcgcttcgac ccgggtgccg gtgacggtgc
      781 tgcgggcggc gatgatcatc ggctcgggga gcgcctcgtt cgagatgctc cgctatctcg
      841 tcgagcggct tccggtcatg gtcacgcccc gctgggtgcg aaccgagtgc cagccgatcg
      901 cgatccgcga cgcgctgcat tacctcgtca catccctcga gacgcccgag acgaccggcc
      961 ggacgctcga catcgggggc cgcgacgtcg tgacctaccg cgagctcatg gacgagacgg
     1021 ccgccgctct cggtctttcg gagcgactgg tgctgcccct cccggtgctg acccccgggc
     1081 tgagctccct gtggatccac ctggtgacgc cgctgtcgca ccgcatcgca cgcccgctca
     1141 ccgaggggct ccgtaatcgc gtcgtctgtc gggacgacgc cgccgctcga ctcatgccga
     1201 ccgagctgct ctcggcggcc gaagcgatcc gcctggcgct gcgggcgtcg cgacagccca
     1261 acgtcgccac ctcctggtcc gacgccgggc ccgtgcccgg cgatccggag tgggcgggcg
     1321 gcaccgcctt ccgagacgag cgccaggcag ctgtcgacgc cggccccggg accgcgttcc
     1381 gcgcgatccg ccgcatcggc ggtggcaacg ggtggtacgc ggccgacctg ctgtggcgga
     1441 tccgtgggtc gctcgaccgc ctcgtcgggg gccctggtct gcgccgaggg cgacgcgacc
     1501 ccgaggtgct gcgccacggc gacgcactcg acttctggcg ggtcgtcgag gtcgagcccg
     1561 accggcgctt gcgcctgcgg gcagagatga agctgccggg ggtggcgtat ctcgagttcg
     1621 acgtcgagcc ggtggggagc ggatcgagaa tcgtccagac cgccgtgttc caccctcgcg
     1681 gcctgttcgg tctcctctac tggtacgccg tcctgccctt ccacggcttc gtgttctcgg
     1741 gcatgctgcg cggaatcgtc cgcactgccg aggaagatgg cgcgtcgacc gcggccgatc
     1801 gcggccccgc atcgccagcg tcccctgcgt cgcgctgacc gctgaccggt gggcggcgtg
     1861 gcgtccgttg tgtgcatcgg cgaccggtag gtcggtcggc ggcggtcaga tgcgagcggc
     1921 gacgacggag aggaggagga cgccgcgggc cgtccacgcg gcggtacgaa gccagttgct
     1981 cgccacgaga gtgcgaacgg tgcggtcgtc gtagccgtgg gagaggcggg cgtgccgcgg
     2041 cacttgaagt agagcggtcg acagccagac gacggcgagc agcgcggcgc ccgagagggc
     2101 gatcccagga ccgagccagg ctggaggcga gacgagccac gcggtcgctg ccgccgcctc
     2161 tccgagcatg agcggcacca cgagcagccc gatgcggtgc gagtggcggc gatgatatgc
     2221 ggcgaagccg tgctcgccca cggcggtgaa gagcgggtaa tgaacgattt ggacgacgag
     2281 aatgaggccg acgagcggga gggtggtggc gagctgcccg agcagcgccg cgtcacccac
     2341 cggcgatctc gccccgcacg gggagtcgcg ccgctcgact catcgccgag cctcggtcga
     2401 gcgatcttcg gcatcggcgc ggcgctcccc gagcgcggcc agacggcgca cctcgatgac
     2461 tctgaagacg aagcctccga ggcagaggct cgagacgaat aggccgacga agaag
//
LOCUS       DBNGNK010000151         6049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_240215,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000151 DBNGNK010000000
VERSION     DBNGNK010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_240215"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1440)
                     /locus_tag="AC150V_04315"
     CDS             complement(157..1440)
                     /locus_tag="AC150V_04315"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR3762178.1"
                     /translation="MSGETLTITDNRTGREYTVPIVDGTIRAPDLEQITVADGDGGLM
                     SYDPAFLNTAACRSAITYIDGDAGILRYRGYPIEQLAEQVSFLETAYLLFEGELPDAD
                     QLRRWEDDVRSHTYVHTNVQKFLEGFRYDAHPMGMVLGAVGALSTFYPDAKQIHDPEN
                     RYLQRVRLMAKVPTIAAFAFRHSHGLPYVLPRNDLEYISNFVNMTFEIGGRYEPNPVL
                     ERALEILLILHADHEQNCSTSAVRSVGSSQVDPFSAVSAGIAALYGPLHGGANEAVLR
                     MLDEIGDTREVPAFIEQVKAGKGRLMGFGHRVYKSYDPRAKLIKRIADLVFEQTGLNP
                     KLEIALELERVALEDDYFVSRKLYPNVDFYSGLIYQAMGYPTDYFTVLFTLGRLPGWI
                     AQWEEMLNDPEQKIARPRQIYVGEAERAFQRPAPN"
     gene            1792..2058
                     /locus_tag="AC150V_04320"
     CDS             1792..2058
                     /locus_tag="AC150V_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3762179.1"
                     /translation="MAPPPEHLRGAVLIRVDRCKGCELCIEYCPVGVLALSKGFNVEG
                     YHHPVVLSDGCISCQACSAICPEFAIFALPAQLLPRARELGLAI"
     gene            2068..3255
                     /locus_tag="AC150V_04325"
     CDS             2068..3255
                     /locus_tag="AC150V_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR3762180.1"
                     /translation="METSSAMAGTHFLLGDNAIAEGAIAAGCRFFGGYPITPSTEVAE
                     RMAQRLPQVGGLYVQMEDEIASLAAVLGAAWTGHKAMTATSGPGFSLMMENLGLGILT
                     ETPCVICNVQRGGPSTGLPTSVSQADMMQARWGSHGHYEIVAVVPWSVQECFDLAVRA
                     FNIAERYRTPVLVMTDAEVGHLTEKLVVPPPGELELWSRKGPQKPPSEFKGYEPDDDL
                     VPPMALAGEGYNVFFESLTHDERGFPLTTPAAQQVLVTRLTEKIRRHRFDIYDWVEDG
                     VDDADVVVISYGISARIAVRAIAEARERGIRTGLLRLRTAWPFPEERVRQLSNRARGF
                     VVPEINLGQMVREVERCAVDECGLLCVPHAGGEIHDVEEIVEAIVRTSEGRAPTRAAL
                     VES"
     gene            3259..5277
                     /locus_tag="AC150V_04330"
     CDS             3259..5277
                     /locus_tag="AC150V_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3762181.1"
                     /translation="MSRVGVFVCHCGENIRRKVDPVHVAEFARRLPGVALAEDYPYMC
                     SAPGQKKVQDAIREQGLTSVVVAACSPHLHEATFRGAAAEAGMNPFLCEMANIREQCA
                     WVTENGGEATDKACEIVATMIEKAKRDQALNSIEVPMTKRALVVGGGVAGIRAALDIA
                     NAGHAVTLVERQPTLGGNMARLSETFPTLDCSQCILTPLMVEASRHPNVELHTYAEVE
                     EVSGYVGNFQVKIRKKARYVNEDLCTGCDDCVVACPATVPNEFDLNLNWRKAIYISFP
                     QAVPAIYTLDAEACVNSNLASQDGGYRVLACERCSDVCGPNAIEYDQQPEILERDVGA
                     IVLASGYELMRAEQAREYGYGRYPDVIDGLEFERLLSASGPTEGLPRRPSDGEVPKSV
                     VFIQCVGSRDPKGGVPYCSRICCMYTAKHALLLRHKVHDSRAVIFYMDIRAAGKGYEE
                     FVQRAMEEEHVLYVRGRVSRVTQHDGKLRVYGTDTLSGQQVEVEADLVVLATAVVPAP
                     NSELARKLRTPTDTDGFFQEVHPKLRPAETLTAGVFLAGSAQAPKDIPDTVAQASAAA
                     SKALELLSRPVLEREPTVARVDESVCIGCFDCEGVCPYRAIVRQEILDREGNLVRTVA
                     QVNEAMCEGCGACTAACRVRAVDVAGFDDEQVFAQLRALASEPVGGPA"
     gene            5274..>6049
                     /locus_tag="AC150V_04335"
     CDS             5274..>6049
                     /locus_tag="AC150V_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR3762182.1"
                     /translation="MSEPIAATVAWEPRIVALVCNWCTYTGADMAGTSRRDHAANVRI
                     VRFLCTGRLDPLFIVKAFENGADAVLASGCHPGDCHYVQGNYLARRRFTVFRSLMEFL
                     GLDPRRLHFAWVSASEGTKWAATVDQVTEVVRELGPVGRWAAPSYEGLEQFTLPEPDG
                     EPRAPTETARLTEQLRETAATLLAQGDTSVVLGYGEGSLPGQRVPVVVTEPTEAARLV
                     WDESCAANLTVYLKEAVRRRRGDGKIAVVVKSCDAKVVTGL"
BASE COUNT          986 a         2000 c         2060 g         1003 t
ORIGIN      
        1 cggcctctgc cctcgtcgcc cgctgaacaa gctggttgcg tagcagcaac ggctctcttc
       61 cgcttgccag agacgtttga ggcgcctcag gccgtgctca gagcggggac tatattctcg
      121 acgcgcactg accttctgcg ggccggaatg gaggcttcaa ttcggtgccg gtcgttggaa
      181 ggctcgttct gcttctccga cgtagatttg gcgggggcgg gcgatcttct gttcggggtc
      241 gttcagcatc tcttcccact gggcgatcca gccggggagc cggccaagtg tgaaaagcac
      301 ggtgaagtag tcggtcgggt agcccattgc ctggtagatc agccccgagt agaagtcgac
      361 gttcgggtag agcttgcgtg agacgaagta gtcgtcctcg agggcgacgc gctcgagttc
      421 gagcgcgatc tcgagtttgg ggttgagacc cgtctgctca aaaaccagat ctgcgatccg
      481 cttgatcagc ttggcgcggg ggtcgtagct cttgtagaca cggtggccga aacccatcag
      541 ccgcccctta cccgccttga cctgctcgat aaaggcggga acctcgcggg tgtcgccgat
      601 ttcgtcgagc atccgcagga cggcctcgtt ggctccgccg tggagcgggc cgtagagtgc
      661 cgcgatcccg gccgacacag ctgagaacgg gtcgacctgc gacgagccga cgctgcggac
      721 ggcgcttgtc gagcagttct gctcgtggtc ggcgtgcagg atgagcagga tctcgagggc
      781 gcgttcgagc accgggttcg gctcataccg cccgccgatc tcgaacgtca tgttcacgaa
      841 gttgctgatg tactccagat cgttccgcgg taggacgtac ggcaagccgt gcgagtgccg
      901 gaacgcgaag gccgcgatcg tcggcacctt cgccatcagc cgcacacgct ggaggtaacg
      961 gttctccgga tcatggatct gcttggcgtc cgggtagaac gtcgacagcg ccccgaccgc
     1021 accgagcacc atgcccatgg ggtgcgcgtc atagcggaac ccctccagaa acttctggac
     1081 gttcgtgtgg acgtaggtgt gcgatcgaac gtcatcctcc cagcggcgca actggtcggc
     1141 gtccgggagc tcgccctcga acagcaggta ggcggtctcg aggaagctga cctgctcggc
     1201 caactgctcg atcgggtagc cgcggtagcg gaggatcccc gcgtcaccgt cgatgtaggt
     1261 gatcgcactg cggcaggccg ccgtgttcag gaacgccgga tcgtacgaca tcagtccgcc
     1321 gtccccgtcg gccaccgtga tctgctccag atccggcgcg cgaatcgtgc cgtcaacgat
     1381 cggcaccgtg tactcccgcc cagtgcggtt atccgtaatc gtcagcgtct cgccagacac
     1441 aacagcctcc tttcgccaaa cctccagtga aacggatcgt ctcagaactc ggccgatcgc
     1501 cgcgctcctc aaccgtcagt acggtcagct cgcttcggga aagctgggag gctcgcagct
     1561 gagtcttgct ccatccgatc acggctcgag cagcgggctc cgtacttctc cgcgcccgga
     1621 accgcgaaag caccgacggc aggggtcccc gagtcctccg atgcttgaag gtgtgcttcg
     1681 gaggccgtaa gttgagcacg gtctggctgg ggtcgcagat ccgagcgggc catgcgtgac
     1741 tccgaaacgg cgcgaaatgt cggtgtgcgc gcagcgaggc agcggtcgcc gatggctcca
     1801 ccgcccgagc atctccgcgg tgccgtgctc atccgggtcg accgctgcaa gggctgcgag
     1861 ctctgcatcg agtactgccc agtgggcgtc ctcgccctgt cgaagggctt caacgtcgag
     1921 ggctaccacc acccggtcgt cttgagcgac ggctgcatca gctgccaggc gtgcagcgcg
     1981 atctgtccgg agttcgcgat cttcgccctc cccgcccaac tgcttccgcg cgcgcgagag
     2041 ctaggcctgg ccatctgagg aggccgcatg gagacgtctt cagccatggc cggaacgcac
     2101 ttcctgctcg gggacaacgc gatcgcggaa ggcgcgatcg ccgccggctg ccgcttcttc
     2161 ggcggctatc cgatcacgcc gtcgaccgag gtcgcggagc gtatggcgca gcggcttcca
     2221 caggtcggcg gcctgtacgt gcagatggag gacgagatcg cctcgctcgc tgcagtgctc
     2281 ggcgccgcct ggaccggcca caaggcgatg accgcaacct cgggccccgg cttttcgctg
     2341 atgatggaga acctcgggct gggcattctc accgagacgc cgtgcgtcat ctgcaacgtg
     2401 cagcgtggcg gcccctcgac cgggctgccg acgtcggtct cgcaagcgga catgatgcag
     2461 gcccgctggg gatcgcacgg gcactacgag atcgttgcgg tcgtcccgtg gtcggtccaa
     2521 gagtgctttg acctggccgt ccgggcgttc aacatcgctg agcggtaccg gacgcccgtg
     2581 ctggtcatga cagacgccga ggtggggcac ctgacggaga agctcgtcgt gccgccgccg
     2641 ggcgagctcg agctctggag ccggaagggg ccccagaaac cgcccagcga gttcaagggc
     2701 tacgaaccgg acgacgacct tgtccccccg atggcgctcg caggcgaagg ctacaacgtc
     2761 ttcttcgaga gcctcaccca cgacgagcgg ggctttccgc ttacaactcc ggccgcgcaa
     2821 caggtccttg tgacgcggct gaccgagaag atccgtcgtc accgcttcga catctacgac
     2881 tgggtcgagg acggtgtcga cgacgcggac gtcgtcgtga tctcgtacgg aatctcggcg
     2941 cgtatcgccg tccgcgcgat cgccgaagcc cgcgagcgtg gaatcagaac cggcctgctc
     3001 cgactccgca cggcgtggcc cttcccggag gagcgcgttc ggcagctctc gaaccgcgcg
     3061 cgcggcttcg tcgtgccaga gatcaacctc ggccagatgg tgcgcgaggt ggagcgctgc
     3121 gcggtcgacg agtgcggcct cctttgcgtg ccgcacgccg gcggtgaaat ccatgacgtc
     3181 gaggagatcg tcgaagcaat cgtccgaacc agtgagggtc gcgcgcccac ccgggccgcg
     3241 ttggtggagt cctagcccat gtcgcgagtg ggcgtcttcg tctgtcactg cggcgagaac
     3301 atccgccgaa aggtcgaccc tgtccacgtc gcagagtttg ctcgtcgact accaggcgtc
     3361 gccctcgccg aggactaccc ctacatgtgc tccgcgcccg gccagaagaa agtgcaggac
     3421 gcgatccgcg agcaggggct gacgagcgtc gtcgtcgcgg cttgctcgcc tcacctgcac
     3481 gaggcgacgt tccgcggcgc ggcggcggag gccgggatga acccgtttct ctgtgagatg
     3541 gcgaacatcc gcgagcagtg cgcatgggtg accgagaacg gcggcgaggc gaccgacaag
     3601 gcctgcgaga tcgtcgcgac gatgatcgag aaggccaagc gcgaccaagc gctcaattcg
     3661 atcgaggtgc cgatgacgaa gcgagcgctg gtcgtcggcg ggggcgtcgc gggcatccga
     3721 gcggcgctcg acatcgcgaa tgctggtcac gccgtcacgc tcgtggagcg gcagccgacg
     3781 ctcggcggga acatggcgcg cctctcggag acgttcccga ccctcgactg ctcgcagtgc
     3841 atcctcacgc cgctgatggt cgaggcctcg cggcacccga acgtcgagct gcacacgtac
     3901 gccgaggtcg aggaggtctc gggctacgtc ggcaacttcc aggtgaagat ccggaagaag
     3961 gcacgctatg tcaacgagga tctctgcacc ggttgcgacg actgcgtcgt cgcctgcccg
     4021 gcgacggtgc cgaacgagtt cgacctgaat ctcaactggc ggaaggcgat ctacatctcg
     4081 tttccgcagg cggttcccgc gatctacacg ctcgacgccg aggcgtgtgt caactcgaac
     4141 ctcgcctccc aggacggcgg ctaccgggtg ctggcctgcg agaggtgctc ggacgtgtgc
     4201 gggccgaacg caatcgagta cgaccagcag cccgagatcc tcgagcgaga cgtcggtgcg
     4261 atcgtcctcg ccagcggcta cgagttgatg agggcggagc aggcgcggga gtacggctac
     4321 ggccgctatc cggacgtgat cgacgggctc gagttcgagc ggttgctctc ggcttcgggt
     4381 ccgactgaag ggttgccgcg gcggccctcg gacggcgagg tgccgaagag cgtcgtcttc
     4441 atccagtgcg tcggctcgcg cgacccgaag ggcggcgtcc cgtactgctc tcggatctgc
     4501 tgcatgtaca cggcgaagca cgcgctcctc ctccgccaca aggtgcacga cagccgcgcc
     4561 gtcatcttct acatggacat ccgcgcggcc gggaagggct acgaagagtt cgtgcagcgg
     4621 gcgatggagg aggagcatgt cctctacgtc cgcggccggg tcagccgcgt cacgcagcac
     4681 gacggaaagc tgcgtgtgta cggcacggac acactgtcgg ggcagcaggt cgaggtcgag
     4741 gcggacctcg tcgtgctcgc gacagcagtc gtgcccgcgc cgaactcgga gctcgcgcgc
     4801 aagttgcgta cgcccacgga cacggacggc ttcttccagg aggttcaccc caagctccgg
     4861 ccggccgaga ccctgacggc gggcgtcttc ctcgcgggct ccgcccaggc gccgaaggac
     4921 ataccggata cggttgcgca agcttccgcg gcggccagca aggcgctcga gctcctctcg
     4981 cggccggtgc tcgagcgtga gccgactgtc gcccgcgtgg acgagtccgt ctgcatcggc
     5041 tgcttcgact gcgagggcgt ctgcccgtat cgggcgatcg tgcggcagga gatcctcgac
     5101 cgcgagggca acctcgtccg aacggtggca caggtgaacg aggcgatgtg cgagggctgc
     5161 ggcgcctgta ccgccgcctg ccgcgtgcgc gccgtcgacg tcgccggctt cgacgacgag
     5221 caggtctttg cgcagctcag ggcgctcgcg tccgagcccg taggagggcc ggcgtgagcg
     5281 agcccatcgc cgcgaccgtc gcctgggagc cgcgcatcgt cgcgctcgtc tgcaactggt
     5341 gcacctacac gggagcggac atggccggca cctcgcggcg cgaccacgcc gccaacgtcc
     5401 gcatcgtgcg cttcctctgt acggggcggc tcgacccact cttcatcgtc aaagcgtttg
     5461 agaacggcgc cgacgcggtt ctcgcgtccg gctgccaccc cggcgactgc cactacgtcc
     5521 agggcaacta tctcgcccgc cggcgtttca cggtcttccg gtcgctgatg gagtttctcg
     5581 ggctcgatcc gcggcggctc cacttcgcct gggtatccgc gtcggaggga accaaatggg
     5641 cggcgacggt tgaccaggtg accgaggtcg tgcgcgagct cgggccggtc gggcgttggg
     5701 cggcgccctc gtacgagggg ctggaacagt tcacgctgcc cgagccggac ggcgagcccc
     5761 gcgcacccac cgaaaccgcc cggctcaccg agcagctccg tgaaaccgca gcgaccctgc
     5821 tcgctcaggg cgacacctcc gtcgtgctcg gctacggcga gggctcgctg cccgggcagc
     5881 gggtgcccgt tgtcgtgacg gagccgaccg aggcggcccg gctcgtctgg gacgagagct
     5941 gcgcggcgaa cctcaccgtc tacctgaaag aagccgtacg gcgcaggcgc ggcgacggga
     6001 aaatcgccgt cgtcgtcaag agctgcgacg ccaaggtggt aacgggcct
//
LOCUS       DBNGNK010000152         3492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_885872,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000152 DBNGNK010000000
VERSION     DBNGNK010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_885872"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="AC150V_04340"
     CDS             <1..1607
                     /locus_tag="AC150V_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3762183.1"
                     /translation="FNEGLDFSCVLFDRRGNMIGQAEFCPAQIAASLFIVRWTLEELG
                     IDSFEEGDVVLHNDPYRGGAHIPEHSVIRPVYHEGELFGFAANVGHLAEIGGKAVGGF
                     AADATEVFQEGLRIPPVKIVKRDKHDEELWKLIMSNHRTPRNTWGDLNAQIGSLRVAE
                     RRVGELLDRFGRSFIEQAADELMDYSERWMRAEIAAIPDGVYSFTERMEDDGVVDRPV
                     TFAVTVTVDGDELIVDWTGTDPQVRGPVNATYGVTAGATYNAVFHLTDQNIPKNSGAY
                     RPIRIIAPPGSAVNVVYPGPSVGGNTETHPKLADMVIAALADALPHRVAAAEGGTACN
                     FLFGGVHPKTGDYYANYHLEGSGWGATAAADGNDAIIVKNGNCRNTPVEIFETRYPLR
                     VVEYSLIPDSGGPGRTRGGLGTRRVFRIGEGATVTVNALLDRTKPGFGAWGLNGGGQG
                     AQGAILVKPAGESEFRTFTDVFGTVSPSKFTNIVLEPGDEVLIDSPGGGGYGDPAERD
                     PERVANDIRQGFVSSTAARDAYGSAG"
     gene            1607..1798
                     /locus_tag="AC150V_04345"
     CDS             1607..1798
                     /locus_tag="AC150V_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762184.1"
                     /translation="MGAWVKLEPQIWETVVTPCECCGQVVARQLWRAEVDGSERNFCG
                     PACEELYRTYVLPRRAQES"
     gene            1795..2706
                     /locus_tag="AC150V_04350"
     CDS             1795..2706
                     /locus_tag="AC150V_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3762185.1"
                     /translation="MRPVELPTPIQREEVRALLPPIGEQIDLVEQTYRSMASGRVEMP
                     PKIGIHPRGDAFLHAMPAYLRDADVAALKWVSGFPANPDKGLPYISGVLVVNDPETGE
                     PIAIMDAAEITAARTAAASGVCIRAWAPAGWSRAALLGCGEQGRYHAAVLRTLNPGVE
                     LAAYDPVAERAEAVAGGVTAAASPEEAVAGSDVIVTAGPIVDDPPSPLVTSWLGDRWL
                     LLPIDFDFYVSAEAVGGSDLFLTDDVGQFEAYRTAGHFRGWQEPATSVGRALGTGAGG
                     DRVVACNLGVAALDAAFAAHVLGGRSA"
     gene            2774..>3492
                     /locus_tag="AC150V_04355"
     CDS             2774..>3492
                     /locus_tag="AC150V_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762186.1"
                     /translation="MRSLRLHHVPLAFAAAALVGAAVAADSARGGTIWHATRAIAERD
                     VKERFSNIESVSCEADRASSTLAKGRVRLWNRFECEGRTYDSVAFALVYRATGECKPC
                     WRMSALRGTGAAHLRQKAAVLPKRPPGPPPPPTPPPPPATSGRAALASEPFARCAPSY
                     RRAGERMRFRSGLEDGEFLLLKDGTVWQTSPGLRSKTESWNRFHTVVLIDGQFGYPCT
                     MVNLNRGERVPVRLAAPGLCGG"
BASE COUNT          522 a         1196 c         1242 g          532 t
ORIGIN      
        1 tcttcaacga gggcctcgac ttctcgtgcg tcctcttcga ccggcgcggg aacatgatcg
       61 gccaggccga gttctgtccg gcgcagatcg cggccagcct cttcatcgtc cgctggacgc
      121 tggaggagct gggcatcgac tcgttcgagg agggcgacgt cgtgctccac aacgacccct
      181 accggggcgg cgcccatatc cccgagcaca gcgtgatccg ccccgtgtac cacgagggcg
      241 agctgttcgg cttcgccgcc aacgtgggcc atctggcgga gatcggcggc aaggccgtcg
      301 gcggcttcgc ggcggacgcg acggaggtgt tccaggaggg cctgcgcatt ccgcccgtga
      361 agatcgtcaa gcgcgacaag cacgacgaag agctctggaa gctgatcatg tcgaaccacc
      421 gcaccccgcg gaacacgtgg ggcgatctga acgcgcagat cggctcgctc cgggtcgcgg
      481 agcgccgagt cggggagctg ctcgaccggt tcgggcgctc cttcatcgag caggcggcgg
      541 acgagctgat ggactactcc gagcgctgga tgcgggccga gatcgccgcc atcccggacg
      601 gcgtctactc gttcaccgag aggatggagg acgacggcgt cgtcgaccgt cccgtcacct
      661 tcgccgtcac cgtcaccgtc gacggcgacg agctgatcgt ggactggacg ggaaccgatc
      721 cgcaggtgcg cggacccgtg aacgcaacct acggagtgac cgcgggcgca acctacaacg
      781 cggtcttcca cctgacggat cagaacattc cgaagaactc aggcgcttat cgtccgatcc
      841 ggatcatcgc gccgccgggc tcggccgtga acgtcgtcta tcccgggccg tcggtgggcg
      901 gcaacaccga gacccatccc aagctcgcag acatggtgat tgcggcgctc gccgacgccc
      961 tgccgcaccg tgtcgccgcg gccgaagggg gcaccgcctg caacttcctc ttcgggggcg
     1021 tccacccgaa gaccggcgac tactacgcga actaccacct cgagggaagc ggctggggag
     1081 cgaccgcggc ggcagacggc aacgacgcga tcatcgtgaa gaacggcaac tgccgcaaca
     1141 cgccggtcga gatcttcgag acgcggtatc cgctgcgggt cgtcgagtat tcgctgattc
     1201 cagactccgg cggtcccgga cgcacccgtg gcggcctcgg cacgcgtcgc gtgtttcgca
     1261 tcggcgaggg cgcgaccgtc acggtcaacg cgctgctcga ccgcaccaag ccgggcttcg
     1321 gggcttgggg cctgaacggc gggggccagg gcgcccaggg tgccattctc gtcaagcccg
     1381 cgggcgagtc cgagttccgc accttcacgg acgtgttcgg gacggtctcg ccgtcgaagt
     1441 tcacgaacat cgtgctcgag ccgggtgacg aggtgctgat cgactccccc ggcggcggcg
     1501 gctacgggga tccggccgag cgtgatcccg agcgagttgc caatgacatc cggcagggct
     1561 tcgtgtcctc tacggccgcc cgcgacgcgt acgggagcgc tggctgatgg gtgcgtgggt
     1621 gaagctcgag ccgcagatct gggagacggt cgtgacgccc tgcgagtgct gtgggcaggt
     1681 cgtcgcgcgg cagctgtggc gggctgaggt cgacggctcg gagcgcaact tctgcggccc
     1741 ggcgtgcgag gagctctatc ggacatacgt gctcccgcgg cgcgcgcagg agtcgtgaga
     1801 ccggtcgagc tcccgacgcc catccagcgc gaagaagtgc gcgcgctgct cccgccgatc
     1861 ggggagcaga tcgacctcgt cgagcagacg taccggtcga tggccagcgg gcgtgtcgag
     1921 atgccgccga agatcgggat ccatccccga ggagatgcgt tcctccacgc gatgcccgcg
     1981 tatctgcgcg acgccgacgt cgcggcgctg aagtgggtgt ccggcttccc ggccaatccg
     2041 gacaaggggc tgccgtacat ctcgggcgtc ctcgtcgtga acgaccccga gacgggtgag
     2101 ccgatcgcga tcatggacgc ggccgagatc accgccgcgc gtactgccgc cgcgagcggc
     2161 gtctgcattc gcgcctgggc gccggccggg tggagcaggg ccgcgctgct cggctgtggc
     2221 gagcagggtc gctaccacgc cgccgtgctg cggacgctca accccggtgt ggagctcgcg
     2281 gcgtacgacc cggtggccga gcgggcagag gcggtggcgg gcggcgtgac ggctgcggcg
     2341 agtcccgagg aggcggtcgc cgggagcgac gtgatcgtga cggccgggcc gatcgtcgac
     2401 gacccgccct ctccgctcgt cacctcctgg ctcggcgaca ggtggcttct ccttccgatc
     2461 gacttcgact tctacgtctc cgccgaggcg gtcggtggct ccgatctatt cctcacggac
     2521 gacgtcgggc aattcgaggc ctaccggaca gccgggcatt tccggggctg gcaggagccg
     2581 gccacgtcgg tcggacgggc gctcggcacc ggagccggcg gcgaccgcgt cgtggcgtgc
     2641 aacctcggcg tcgccgcgct cgatgcagcg ttcgccgccc acgttctggg cggccgcagc
     2701 gcctgagcgg caacaacgag gaatgcccgg cccggggtcg tagttgcgag agatcctcga
     2761 ctcaggccgc tcgatgcgct ccctccgtct gcaccacgtc ccgctcgcct tcgccgccgc
     2821 tgcgctggtc ggagcggccg ttgcagccga ctctgcgcgg ggcgggacga tctggcacgc
     2881 gacgcgtgcg atcgccgagc gggacgtcaa ggagcggttc tcgaacatcg agtccgtgag
     2941 ctgtgaggcc gaccgcgcca gctcgaccct cgccaagggc cgggtgcgtc tgtggaatcg
     3001 cttcgagtgc gaggggcgga cgtacgactc ggtcgcattc gcgctggtct atcgggccac
     3061 cggcgagtgc aagccgtgct ggcggatgtc cgctctccgc ggcaccgggg ccgcacacct
     3121 cagacagaag gcagcggtgc tgcccaagcg ccccccgggt cctcctccgc cgccgacgcc
     3181 accaccaccg cccgcgacat ccggccgtgc ggcgctcgcc agcgagccct tcgctcgctg
     3241 cgcgccaagc tatcgacggg ccggcgagcg catgcgtttt cgctccgggc tcgaggacgg
     3301 cgagttcctg ctgctcaagg acggaacggt ctggcagacg agcccgggtc tacgctctaa
     3361 gaccgagtcg tggaatcgct tccataccgt cgtgctgatc gacgggcaat tcgggtatcc
     3421 ctgcacgatg gtgaacctca accgtggcga gcgggtgccg gtgcggcttg ctgcgcccgg
     3481 tctctgcggc gg
//
LOCUS       DBNGNK010000153         3195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1172023,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000153 DBNGNK010000000
VERSION     DBNGNK010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1172023"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="AC150V_04360"
     CDS             <1..344
                     /locus_tag="AC150V_04360"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR3762187.1"
                     /translation="LDDVLRQLEERDAPLCESCGSVLKPGVVMFGELLPEAAIDRATQ
                     LAREAGLLLVVGSSLQIWPVAGLPEETLRAGGRLAILNREPTPYDEHAELTLAREAGE
                     TLAEVERILIS"
     gene            408..707
                     /locus_tag="AC150V_04365"
     CDS             408..707
                     /locus_tag="AC150V_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762188.1"
                     /translation="MTETEAQTETEPDAEADDKDTIGRLADRGEETIRRLVDLPGGHR
                     ALKAFNALRVRVDELNRKVRGIDALEARLAALEARVDALDGGPPEEAEETREASK"
     gene            complement(774..1736)
                     /locus_tag="AC150V_04370"
     CDS             complement(774..1736)
                     /locus_tag="AC150V_04370"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR3762189.1"
                     /translation="MTADDRVVFLVNPASDNGRTGRRWPELAHRAAAAGLDADALLSE
                     APGHLAELARGAALAGAELLVVVGGDGSVYEAANALADLPDPPALAVVARGTGWDFVR
                     TYGIPRRIEDAVLVATRGDTRTIDLGRVVYRRWDGGETEGVFANIASVGMSGAIAKRA
                     NETSKAMGGKPSYLWATLAVFTGWAGTELRVTVDEETRSGKMFDAVVANGRYFGGGMK
                     ICPEAEPDDGLFDVLLIGDITKRDLLLTMPKIYRGTHLPHPKGELLRGRRVTVEAREH
                     LPVQLDGEQPGTAPVRFDVLPRALRLRVPAATAGGDPPNRTRSK"
     gene            complement(1741..2454)
                     /locus_tag="AC150V_04375"
     CDS             complement(1741..2454)
                     /locus_tag="AC150V_04375"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoD-type purine-nucleoside phosphorylase"
                     /protein_id="HIR3762190.1"
                     /translation="MPIHVRAEPGEYAEACLLPGDPLRAKYIAETFMDDVVERNSERG
                     MLGYTGTFDGKPVSVQASGMGCPSAAIVIEELAQLGVKKIVRVGTCGGLQADLEIGDM
                     IIALSSAPADSTALHYIGNEPHVPTADFELVHGAVHHAKKLGKPVRVGPIVSSDIFYQ
                     PDPEQVNRWSARGLLAVEMEAAILFTLGALRNLQAGCLLIVSDVIVEGEFVRISDEDM
                     RRAVDEMTELALHVATDDH"
     gene            2485..>3195
                     /locus_tag="AC150V_04380"
     CDS             2485..>3195
                     /locus_tag="AC150V_04380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3762191.1"
                     /translation="MSKLVEPTKEQILAYCQADPIERVFLEDVARRGHGRFAGVGDGN
                     GITGLCHLGTNVVPSGLGCGAFAGLATRSGARMIIGEQGAVSDLWSVAGGRLPEPRED
                     RPGQPVYALAESPEPGGTKLRPARIRDLDLLVPACAAAHEEELGVDPLRRDREGFRWR
                     TRVQIEEGRSWIWVEDGVILFKAEASAWTPSAVQLQQVWTDPPARGQGHATRALRDLV
                     RLLLDEVPVVCLFVRADNA"
BASE COUNT          490 a         1092 c         1109 g          504 t
ORIGIN      
        1 cgctcgacga cgtgctccgt cagctcgagg agcgcgatgc acctctctgc gagtcctgtg
       61 gctcggtcct caagccaggg gtcgtgatgt tcggggagct tctgcccgag gccgcgatcg
      121 accgggcgac gcagcttgcc cgcgaagcgg gcctcctgct cgtcgtaggc tcctcgttgc
      181 agatctggcc cgtcgccggc ctacccgagg agacgttgcg cgccggcggg cgcttggcga
      241 ttctcaaccg agagccgacg ccgtacgacg agcacgcgga gctgactctc gcccgcgagg
      301 cgggcgagac tctcgccgag gtagagcgga tcctcatcag ctaggcggcc ccgcagtgcg
      361 gggccgtgca gatgatttag ccgcccagga gtggtatgac tccggccatg accgagaccg
      421 aagctcaaac cgagaccgaa ccggacgcag aggccgacga caaggacacg atcggccgcc
      481 tggccgaccg aggggaggag acgattcgtc gtctcgtcga cctaccgggc ggccaccggg
      541 ccctgaaggc gttcaacgcc ctccgcgtgc gggtcgacga actcaaccgc aaggtacgcg
      601 gcatcgacgc gctcgaagct cgcctggccg cactcgaagc tcgagtcgac gcgctcgacg
      661 gcggcccgcc cgaggaggcc gaagagaccc gggaagcaag caagtaacag tctgttactt
      721 gcttgcctcg atcggctcga atcggacgct gtcgagcagc aagtaacatc ctgttacttg
      781 ctgcgggtcc tgttcggcgg atcgccaccg gcggtcgccg ccggaacgcg gaggcgcaga
      841 gcccgcggaa gcacgtcgaa gcgcacaggc gcggtgccgg gctgctcgcc gtcgagctga
      901 accggcaggt gctcgcgagc ctcgacggtc accctccggc cgcgtaggag ctcgcccttc
      961 gggtgcggca ggtgggtgcc ccggtagatc ttcggcatcg tcaggagcaa atcgcgcttc
     1021 gtgatgtcgc cgatgagcag gacgtcgaag aggccgtcgt cgggctccgc ttcggggcag
     1081 atcttcatgc ccccgccgaa gtagcggccg ttggccacca ccgcatcgaa cattttcccg
     1141 ctgcgcgtct cctcgtcgac ggtgacgcgg agctctgtcc ccgcccagcc ggtgaagacg
     1201 gcgagcgtcg cccacagata ggagggcttg ccgcccatcg ccttcgacgt ctcgttggcc
     1261 cgcttcgcga tcgcgccgct cattcccacg ctcgcgatgt tggcgaagac gccctccgtc
     1321 tccccgccgt cccatctgcg gtacacgaca cgcccgaggt cgatcgtccg cgtatcgccg
     1381 cgggtggcga cgaggacggc gtcctcgatc ctccgcggga taccgtacgt acggacgaag
     1441 tcccagccgg ttccgcgcgc gacgacggcg agtgcaggcg ggtccgggag gtcggcgagc
     1501 gcgttggccg cctcgtagac ggagccgtcc ccgccgacga ccacgaggag ctcggcgccc
     1561 gcgagcgcag ccccgcgggc caactcggcc agatggccgg gggcttcgct cagcagtgcg
     1621 tcggcgtcga gcccggcggc cgcggcgcgg tgcgccagtt ccggccagcg acggcccgtg
     1681 cggccgttgt ccgaggctgg attgacgagg aagacgacgc ggtcgtctgc cgtcatgcgc
     1741 tcagtggtcg tccgtcgcga cgtgcaacgc gagttctgtc atttcgtcga ccgcccgcct
     1801 catgtcctcg tcggagatgc ggacgaactc gccttcgacg atcacgtcgc tcacgatcag
     1861 gaggcaaccg gcctgcaggt tcctcagggc gccgagcgtg aagaggatcg cggcttccat
     1921 ctcgacggcc agcaggccgc gggccgacca gcggttcacc tgctcgggat cgggctggta
     1981 gaagatgtcg ctcgacacga tcgggccgac ccgtacgggc ttcccgagct tctttgcgtg
     2041 gtgaaccgca ccgtgcacaa gctcgaagtc ggcggtgggg acgtggggct cgttgccgat
     2101 gtagtgcagc gccgtcgaat cggccggagc cgacgagagc gcgatgatca tgtcgccgat
     2161 ctccagatcg gcctggaggc cgccgcaggt tccgacgcga acgatcttct tcacgccgag
     2221 ctgcgccagc tcctcgatca cgattgccgc cgacgggcaa cccatcccgc tggcctgcac
     2281 cgatacgggc ttgccgtcga acgtccccgt gtagccgagc atgccgcgct cggagttgcg
     2341 ctcgacgaca tcgtccatga acgtctcggc gatgtacttc gcccggagag gatccccggg
     2401 taggaggcac gcctcggcgt attcgccggg ctccgccctg acatggatcg gcatccgcgc
     2461 aaccctatag ggcaggatca ggctgtgtcg aagctcgtcg agcccaccaa agagcagatc
     2521 ttggcctatt gccaggccga tccgatcgag cgcgtcttcc tcgaggacgt ggctcggcga
     2581 gggcacggcc gcttcgccgg cgtcggtgac ggaaatggca tcaccggcct ctgtcacctc
     2641 gggacgaacg tcgtcccctc gggcctgggc tgtggggcct tcgccggcct ggcgacccga
     2701 tcaggagccc ggatgatcat cggagagcag ggcgccgtca gtgacctctg gtctgttgcc
     2761 ggagggcggc tgccggagcc gcgtgaggac cgcccgggac agcccgtgta cgcgcttgca
     2821 gagtcccccg agcctggcgg gacgaagttg cgtccagcgc gtatacgcga tctcgacctg
     2881 ctcgtcccgg cctgcgcggc cgcgcacgag gaggagctcg gtgtcgaccc gctgcgacgc
     2941 gatcgcgagg gctttcgctg gcgaacccgc gtgcagatcg aggagggtcg ctcgtggatc
     3001 tgggtcgagg acggagtgat cctcttcaag gccgaggcgt cggcatggac gccgtcggcg
     3061 gtgcagctcc agcaggtctg gacggatccg cccgcgaggg gccagggcca cgcgacgcgc
     3121 gcgcttcgtg acctggtccg gctcttgctc gacgaagtgc cggtcgtctg cctcttcgtc
     3181 cgcgccgaca acgcg
//
LOCUS       DBNGNK010000154         4685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_26641,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000154 DBNGNK010000000
VERSION     DBNGNK010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_26641"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1510
                     /locus_tag="AC150V_04385"
     CDS             140..1510
                     /locus_tag="AC150V_04385"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3762192.1"
                     /translation="MASQPVPGTVQARRATAGTRFSAGAVVLAAAVPILFLHVRWQPT
                     WALDIGSTTADVALSDLAVLVVAIVAFREALRSGVQPLRAGLPLWLAGGALLGLVALG
                     VLRPEATADAQFYAHLVSALKFVEYASLAVCVPLLIRRTDDLVPPAVAVALWAGAAAA
                     VAAAQFVGLDVLDAWQAGWRQPSFVGHHDLAALGLLAAAVGSAMFASRSTGPAPPWTM
                     PLALAGGCVALVLSGSVAAAGGFALGVLVLGLAARRRFELDVRRALALAAIVLAVLGG
                     VTALRADPLASFMGFLGVRDETPADEIETYSQRSVLAYVGLRIFADNAVLGVGWQRSD
                     RPEEFLPYIDDARRRFPSVAEQAFPAPGREWGVQNAYVQAAADLGVLGLIAVVGVFAT
                     GLLLAARGARAAAPWAGLGLACLCAIFGLIGLWNALGLVAGIPTLAATALTLGGAATA
                     AAIGRG"
     gene            1507..2208
                     /locus_tag="AC150V_04390"
     CDS             1507..2208
                     /locus_tag="AC150V_04390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762193.1"
                     /translation="MSDPRLRPSRAMPTYAVRAPLARWLATEAARAHRDYGLYRLLDV
                     GCGEKPYEPLFAPFASAYVGVDPVDNPIADLRGTVEALPAADESFDVVLCLQVLEHCD
                     DPALAVAELSRVTAPGGRVLASTHGVMAYHPSPVDYWRWTHAGLERLFAENAVWASVT
                     VTPASGTAACLGMLASLYIDLGARRARVGSLARPLVAAVNATAEAIDRRSPTLSEPGP
                     GTLFANYHVVAEKRT"
     gene            2205..3140
                     /locus_tag="AC150V_04395"
     CDS             2205..3140
                     /locus_tag="AC150V_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3762194.1"
                     /translation="MSRRVLVTGGGGFIGSNLVRALLRRGDEVRILDNFSTGNRDNLA
                     EIGGQIEVVEGDLRSYERVHAAVRGTELVFHQGALPSVPRSVQDPLTTTAVNVEGTLN
                     VLLAARDENVRRVVFASSSSVYGNEGILPRTEDQLPSPISPYAVAKLAAERFCASFSG
                     VYGLETVSLRYFNVFGPHQDPTSQYAAVVPRFIHAIDAGRPVTIYGDGEQSRDFTYVA
                     NVVEANLLAADAVGVSGAILNVATGGSETVNTLADTVGSLLEKPAEKAYEPARPGDVR
                     QSWADIGAAREQLGFQPTVAFEDGLRLTIDSLLGE"
     gene            3160..3549
                     /locus_tag="AC150V_04400"
     CDS             3160..3549
                     /locus_tag="AC150V_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3762195.1"
                     /translation="MSHSFGSIDELGEGYGFRKVRQALGVTAFGVNVVVYPPGQEGFA
                     HYHEEQDELYFVHSGTARFEVAGEERLVGPGGLCHVESTTPRKVSNAGEDDLIMLVVG
                     GKGGYVGRDGQLVDPGDLGRRASFGKD"
     gene            complement(3564..>4685)
                     /locus_tag="AC150V_04405"
     CDS             complement(3564..>4685)
                     /locus_tag="AC150V_04405"
                     /inference="COORDINATES: protein motif:HMM:NF023218.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HIR3762196.1"
                     /translation="AVAVAVALAPTATGSRFMQHGIFDDAQILFGNPDVVFPTLKTLN
                     TKIIRVNLWWGGPFGVAKTRPARATNSLDPAYNWETYDRTAIYAAAYGMRVVFSILGT
                     PTWANGGKDHRTAPLNTRDLRDFTRAAARRYDGTFVGPNGRVLPAVRYWIAWNEPNNP
                     SFLVPQFRQIGKRWVIESARQYARICNAVVKGVKLVKRNRGRVACGATAPRGNNQPTA
                     SRASVSPLAFIRAMKSFGARGFKAFAHHPYYGKKTETPTTPPPQGRRGQPSRAVTLGN
                     FNVLVREINRLYGRGTRIWVTEYGYQTNPPDKIFGVTKRKQREYLEEAYRILKAHPRV
                     DMFLWFLLQDESLLGGWQSGLIAENGRKKPSFPAFRKLN"
BASE COUNT          624 a         1651 c         1644 g          766 t
ORIGIN      
        1 cgtcgagacc gagcgtgcgt gcggcggcga ccgtgccgag aatcccgaag acgaccgacg
       61 cgtagagccc ggcggccgtc gccgaacggc gccagaggag cccggagcgc atctcggacg
      121 gccagtatag tgagtcgtct tggcgtccca gcccgttcca ggcacggtgc aggcgcgccg
      181 cgcaacggct gggacacggt tctcggccgg cgcagtcgtg ctcgccgcgg ccgtcccgat
      241 cctcttcctg catgtccgct ggcagccgac gtgggcgctc gacatcggat cgaccacggc
      301 ggacgtcgcg ctctccgacc ttgccgtcct cgtcgtggcg atcgtcgcct ttcgcgaggc
      361 gctccgttca ggagttcagc cgctgcgggc gggcctgccc ctctggctcg cgggcggcgc
      421 tctcctcggg ctcgtcgccc tcggcgtgct gcgccccgag gcgaccgccg acgcgcagtt
      481 ctacgcccat ctcgtctcgg cactgaagtt cgtcgagtac gcgtccctcg cggtgtgcgt
      541 cccgctgctc atccgccgaa cggacgatct ggtgccaccg gccgtcgccg tcgcgctctg
      601 ggcgggggcg gcagcggccg tcgcggcggc gcagttcgtc ggcctcgacg tcctggacgc
      661 gtggcaggca ggctggcgcc agccgtcgtt cgtcggccac cacgatctcg ccgcgctggg
      721 cctgctcgcc gccgccgtcg ggagcgcgat gttcgcgtcg cgatcgacgg gaccggcgcc
      781 gccgtggacg atgccccttg cgctggcggg cggctgcgtc gccctcgtgc tctcgggaag
      841 cgtcgccgct gcggggggct tcgcgctggg agtgctcgtg ctcggcctgg ccgcccggcg
      901 ccgcttcgag ctcgacgttc gccgggctct ggcgctcgcc gcgatcgtgc tggccgtgct
      961 aggaggcgtc accgccctgc gagccgaccc gctcgcatcg ttcatgggct tcctgggagt
     1021 gcgggacgaa acgcccgcgg acgagatcga gacgtactcg cagcgctcgg tcctcgcgta
     1081 cgtcgggcta cgaatcttcg cggacaatgc ggtgctcggt gtcggctggc agcgttccga
     1141 ccggcccgag gagttcctcc cttacatcga tgacgcccgc cgccgtttcc cgagcgtggc
     1201 cgagcaggcc ttccccgccc cggggcgcga gtggggcgtg cagaacgcgt acgtgcaggc
     1261 cgccgccgat ctgggcgtgc tcggactgat cgcagtcgtc ggagtgttcg ccacggggct
     1321 cctgcttgcg gcgcgcggcg ctcgggccgc cgcgccctgg gctggactgg gactcgcctg
     1381 tctctgtgcg atcttcggcc tgatcggcct ctggaacgca ctcggcctgg tcgccgggat
     1441 tccgacactc gcggccaccg ccctcacgct cggcggagct gccacggctg cggcgatcgg
     1501 acgcggatga gcgatccgcg cctccggccg tcccgggcga tgccgaccta cgcggtccgg
     1561 gcgccgctcg cgcggtggct cgccacggag gcggcacgag cgcatcggga ttacggcctg
     1621 taccggctgc tggacgtcgg ctgcggcgag aagccgtacg agcctctctt tgcaccgttc
     1681 gcctcggcat acgtcggcgt cgacccggtc gacaacccaa tcgccgacct gcgcggcact
     1741 gtcgaggctc tgccggcggc cgacgaaagc ttcgacgtgg tgctctgcct ccaagttctg
     1801 gagcactgcg acgatccggc gctcgccgtc gccgagctga gccgggtcac agcccccgga
     1861 ggccgcgtgc tcgcttccac gcacggcgtg atggcgtacc acccgtcgcc ggtcgactac
     1921 tggcgctgga cgcatgccgg gctggagcgc ctgttcgccg agaacgcggt ctgggcctcg
     1981 gttacggtta cgccggcctc cggaacggcg gcctgtctcg gaatgctcgc ctcgctctat
     2041 atcgacctcg gcgcacgccg cgctcgcgtc ggctcgctgg cccgtcccct cgtcgccgct
     2101 gtcaacgcga cggccgaggc aatcgaccgc cgctcgccca ccctgtccga gcccggcccg
     2161 ggcacactgt tcgccaatta ccacgtcgtg gcggagaagc gcacgtgagc cggagggttc
     2221 tcgtcacagg gggtggcggg ttcatcggct ccaacctcgt ccgcgcgctc cttcggcgcg
     2281 gcgacgaggt taggatcctc gacaacttct ccaccgggaa ccgggacaac ctcgcggaga
     2341 tcggaggtca gatcgaggtg gtcgaggggg atctccgctc gtacgagcgt gtccacgccg
     2401 cggtacgtgg cacggagctg gtcttccacc agggggcgtt gccgtccgtc ccccggtccg
     2461 tgcaggaccc gctgacgacg accgccgtga acgtcgaggg gacgctgaac gtcctcctgg
     2521 ccgcccggga cgagaacgtg cgacgggtcg tgttcgcctc gtcctcgtcc gtgtacggga
     2581 acgaggggat cttgccccgc accgaggatc agctcccgtc gccgatctcg ccgtatgcgg
     2641 tcgccaagct ggccgcggag cgcttctgcg ccagcttcag cggcgtctac gggctcgaga
     2701 ccgtctcgct ccgctacttc aacgtcttcg gcccgcacca ggacccgacc tcgcagtacg
     2761 cggccgtcgt gccgcgcttc atccacgcga tcgacgcggg gcggccggtc acgatctacg
     2821 gcgacggcga gcagtcgagg gacttcacct acgtggcgaa cgtcgtcgag gccaacctcc
     2881 tcgccgcgga cgccgtcggt gtcagcggag cgatcctcaa cgtggccacc ggcgggtcgg
     2941 agacggtgaa cacgctcgcc gacaccgtcg gctcattgct ggagaagccg gccgagaagg
     3001 cgtacgagcc ggcccgaccc ggcgacgtcc gccagtcgtg ggccgacatc ggcgccgccc
     3061 gcgagcagct cgggttccag ccgacggtcg ccttcgagga cgggctccgc ctgacgatcg
     3121 acagcctgct gggcgaatga cgagccaagg aggacggcga tgagccacag cttcggctcg
     3181 atcgacgagt tgggcgaggg atacggcttt cgcaaggtca gacaggcact cggcgtcacg
     3241 gccttcggcg tcaacgtcgt cgtctacccg ccgggccagg aaggcttcgc ccactaccac
     3301 gaggagcagg acgagctcta cttcgtacac tccggaacgg cgcggttcga ggtggcgggc
     3361 gaggagcggc tggtcggccc gggcgggctc tgccacgtcg agtcgacgac gccccggaag
     3421 gtctcgaacg ccggcgagga cgacctgatc atgctcgtgg tcggcggcaa gggcggctat
     3481 gtcggccgcg acggccaact cgtcgacccg ggcgacctcg gccggcgtgc ctctttcggc
     3541 aaagactgag gcggggccgc tcgctagttc agcttgcgga acgcggggaa ggacggcttc
     3601 ttccttccgt tctccgcgat caaccccgac tgccacccgc ccagcagcga ttcgtcctgg
     3661 aggaggaacc agagaaacat gtcgacgcgc ggatgcgcct tgaggatcct gtatgcctcc
     3721 tccaggtact cgcgctgctt ccgcttggtg acgccgaaga tcttgtcagg cgggttcgtc
     3781 tggtacccgt actcggtcac ccagatccgg gtgccccggc cgtacagccg gttgatctcc
     3841 cgaaccagca cgttgaagtt gccgagcgtc acggcccggc tcggctggcc gcggcgcccc
     3901 tgcggcggcg gcgtcgtcgg cgtctcggtc ttcttgccgt agtagggatg atgcgcgaat
     3961 gccttgaatc ccctggcccc gaagctcttc atcgctcgga tgaaggcgag cggcgacacc
     4021 gatgcacggg acgccgtcgg ctggttgttg ccgcgggggg cggtggcgcc gcaggcgacc
     4081 cgccccctgt tgcgcttgac gagcttgacg cccttgacga cggcgttgca gatgcgcgcg
     4141 tactggcgcg cgctctcgat cacccatcgc ttcccgatct ggcggaactg cggcacgaga
     4201 aagctcgggt tgttcggctc gttccaggcg atccagtagc ggaccgcggg gaggactcgc
     4261 ccgttcggcc cgacaaaggt gccgtcgtac cgtcgcgccg cggcgcgcgt gaaatcgcgc
     4321 agatcgcggg tgttgagcgg cgcggtgcga tggtctttgc cgccgttcgc ccaggtcggc
     4381 gtgccgagga tcgagaagac cactcgcatc ccgtaggccg ccgcgtagat cgcggtgcga
     4441 tcgtacgtct cccagttgta tgccggatcg agcgaattgg tcgcccgcgc cgggcgcgtc
     4501 tttgcgacgc cgaacgggcc gccccaccag agattgaccc tgatgatctt cgtgttgagc
     4561 gtcttcaggg tgggaaagac gacgtcagga ttcccgaaca ggatctgggc gtcgtcgaag
     4621 atcccgtgct gcatgaagcg cgagcccgtc gcggtgggcg caagggccac ggccacggcc
     4681 acggc
//
LOCUS       DBNGNK010000155         6018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1243529,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000155 DBNGNK010000000
VERSION     DBNGNK010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6018
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1243529"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="AC150V_04410"
     CDS             complement(<1..396)
                     /locus_tag="AC150V_04410"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3762197.1"
                     /translation="MSSETGPVAGVGAVIVAEGRIVLVQRGRGAYAGAWAVPGGKQRF
                     GETMQEAVAREALEETGLVVKVGDGVWVGDALDDAEPPSWHYVLVDFAATVVGGELRA
                     GDDALAAEWVPLEEARLRRLTPTMPSLLEL"
     gene            complement(393..1331)
                     /locus_tag="AC150V_04415"
     CDS             complement(393..1331)
                     /locus_tag="AC150V_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HIR3762198.1"
                     /translation="MALEVGIVGLANAGKTTLFNALTHAGAEITAYASVTEKPNVGMA
                     TIADDRLGQIARVVGSRQATPAAIRVVDVPGTGPQLLGNLRKVDALLAVTDGFSEGAA
                     PAADLETGELELLVADRDHVERRLERVEKQAKSGDPALRKEVDELRPVLAHLDAGLSL
                     EQWPDALPPELEPLTTMPLLAIENGPHGIDCMLEAELAELPEAEAAELRDGPSALAEI
                     VRRLQDALGLITFFTAGDKEARAWTLRGGQTALEAAAAVHTDIARGFIRCEVIRWDDL
                     VECGSHAEAARRGLQRLEGKAYVVEDGDVLNIRFNV"
     gene            complement(1347..1574)
                     /locus_tag="AC150V_04420"
     CDS             complement(1347..1574)
                     /locus_tag="AC150V_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762199.1"
                     /translation="MPDAERVRIEVAFDGGQILGAFVPAEGADALERALAASSEGTLQ
                     LHTEDGRISVVLSRVLYVKRYARDAQVGFGN"
     gene            complement(1561..1971)
                     /gene="mce"
                     /locus_tag="AC150V_04425"
     CDS             complement(1561..1971)
                     /gene="mce"
                     /locus_tag="AC150V_04425"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR3762200.1"
                     /translation="MHPRRIHHVGMAVADLDEALATYERLFGGRLEHRQTLPPQGVEA
                     VSVLVGEARIELLAPTGADTPVGRYLDRRGPGMHHVAYEVDSVGGALQELRAAGAQLI
                     DETPRPGLFGLEVAFIHPDAVHGVLTEVVSVARR"
     gene            complement(1995..3524)
                     /gene="ftsH"
                     /locus_tag="AC150V_04430"
     CDS             complement(1995..3524)
                     /gene="ftsH"
                     /locus_tag="AC150V_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3762201.1"
                     /translation="MGSMGWAAASFVDLISDWALTWLPILLFIVIIYLLWRTVQYMPR
                     VKPAPVERDSSEKVAWADIAGLNEAKAELQEVVDFLRDRKRFERLGAQVPKGILLYGP
                     PGTGKTLIAKAVASASGANFYSSSASAFVEMFAGLGAARIRKLFEEARKNAPSIVFID
                     ELDAVGTARSGGSFNREHDQTLNQLLVELDGFGGRDQVIVMGASNRLQDLDPALLRPG
                     RFDRQVLVSPPDLAGREEILRVHTRSKPLGADVDLNVIARRTAGLTGADLANIANEAA
                     IFAGRSDQDRIRQADFEAAMERVVAGLQTRRVVTDKEKRILAYHEAGHALMAHLMGDL
                     IPVQKVTIVSRGQALGYTLNMPSEDRYLHTREEFVDLMKIFLSGRAAEHVVFGRITNG
                     AANDLERVTEIARQMVFEFGMSEVAPSRTMRAENYALSEETKRLRDSEQARLTDHAYT
                     EARRLLEKHRPALDRVAGALLEKETLERNELAAMLVDVIPESHSAETVGTVRALSGNA
                     K"
     gene            complement(3547..4203)
                     /locus_tag="AC150V_04435"
     CDS             complement(3547..4203)
                     /locus_tag="AC150V_04435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3762202.1"
                     /translation="MSEPVLWDVDTQVDFVEPHGKLYVPGAEETRGAMARLVQAARAA
                     GLVHVASADDHELTDPEISDAPDFENTYPPHCLRGTGGARKIEATQQDDPLPFSLLPY
                     PPGVVPALVVGRREILLLKKSYSAFTNPNAEPLLRALAPSEIIVFGVATDVCSHAAIM
                     GMLERGYRVAFAEDAAQGLSDDRVASCTALWRQEGVRFTTSHEVAAALESTVRTRTSG
                     "
     gene            complement(4196..4402)
                     /locus_tag="AC150V_04440"
     CDS             complement(4196..4402)
                     /locus_tag="AC150V_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762203.1"
                     /translation="MPRARGGLTEATARVGRRLPAEESGLPEDRIRDGGENDYAADIV
                     LTDGVPSAKVGRRFRTDPGLERVV"
     gene            complement(4378..5148)
                     /locus_tag="AC150V_04445"
     CDS             complement(4378..5148)
                     /locus_tag="AC150V_04445"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR3762204.1"
                     /translation="MEKVRLGGMALANGVLVHGPTAWACAVRLPDGAIRIASGYKPRF
                     GARINAPLLRGPLRLAEAFALLPIVRRRLPEARFPWERPRVAAAVVAGAAVAGAARRS
                     RLSTATREGLAAVASLAPAALALRGGDLASYHGAEHVSIGTYEQDGERAAKEHERCGS
                     HLVGPLLLSSAAAGALAARTPRRTRPAARAAAAVGALGASVEVFAWMTRNPEHPVARA
                     LARPGHELQSRLSTEEPSPEQLEVAEAALAACLELEED"
     gene            5173..5694
                     /locus_tag="AC150V_04450"
     CDS             5173..5694
                     /locus_tag="AC150V_04450"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR3762205.1"
                     /translation="MDATTVLARLAALRPERGFVFVGIGGHGCAGKTTLARSIPGATF
                     VATDEFWDGSEFDLRRLRREVVEPLLEGREARFHSFDWAKQTPRPERRAIRPEGIVVI
                     EGVCALHRMFRADYDLRVWVDTPYELRLRRAIERDGERMRKTWVDVWMPGEERYVAKD
                     DPVAAADLVVSGA"
     gene            complement(5695..>6018)
                     /locus_tag="AC150V_04455"
     CDS             complement(5695..>6018)
                     /locus_tag="AC150V_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR3762206.1"
                     /translation="VDADSTVLEGPVTNVWWRVGRTLRTPALELGILAGVTRQSVLDL
                     APQIGYVVEEGVFALADLLAAEEAFTSSSVRELLPVVELDGQPLHHGPAAGELLAAIR
                     ADAAV"
BASE COUNT          916 a         2141 c         2051 g          910 t
ORIGIN      
        1 cagctccagc aacgagggca tcgtcggcgt gagacgccgc agtcgcgcct cctcgagcgg
       61 cacccattcc gcggcgagcg cgtcgtcgcc ggcccggagc tcgcctccca cgaccgtcgc
      121 cgcgaaatcg acgaggacgt agtgccagct cggcggctcc gcatcgtcga gtgcgtcgcc
      181 cacccagacc ccatccccca ccttcacgac gaggccggtc tcctcgagcg cttcgcgcgc
      241 gaccgcctcc tgcatcgtct cgccgaagcg ctgcttgcct cccggcaccg cccacgcgcc
      301 cgcataggct ccccgaccgc gctgaacgag cacgatgcgt ccctcggcca cgatgaccgc
      361 tcccaccccc gcaaccggac cggtctcgct gctcatacgt tgaaccggat gttgagcacg
      421 tcaccgtcct cgaccacgta cgccttgccc tcgagacgct ggagcccccg ccgagccgcc
      481 tcggcatgtg agccacactc gaccagatcg tcccatctga tcacctcaca gcggatgaaa
      541 ccgcgggcga tgtccgtgtg caccgcagcg gcagcctcga gcgcggtctg gccccctcgc
      601 aacgtccagg cccgcgcctc tttgtcgcct gccgtgaaga aggtgatcag gccgagcgcg
      661 tcctggagcc gtcgcacgat ctcggccagc gccgagggcc cgtcgcgtag ctccgccgct
      721 tccgcctccg gaagctcggc gagctcggcc tcgagcatgc agtcgatccc gtgcgggccg
      781 ttctcgatcg cgagcaacgg catcgtcgtc agcggctcga gctcgggggg cagcgcgtcc
      841 ggccactgct cgagcgacaa gccggcgtcc aggtgggcca gcacggggcg gagctcgtcg
      901 acctccttcc gcagcgccgg atcgccggac ttggcctgct tctcgactcg ctcgagccgc
      961 cgttcgacgt ggtcacggtc ggcaacgagc agctctagct cgccggtctc caggtcggca
     1021 gccggagcgg cgccctccga gaagccgtcg gtgaccgcga gcagcgcgtc caccttgcgc
     1081 aggttgccga ggagctgtgg gccggtgccg ggaacgtcga ccactcggat cgccgccggc
     1141 gttgcctggc gagatcccac gacccgcgcg atctgcccca gccggtcgtc cgcgatcgtc
     1201 gccatgccga cgttcggctt ctcggtcacc gacgcatacg cggtgatctc cgctcccgca
     1261 tgcgtcaagg cgttgaagag cgtcgtcttc cccgcgttcg cgagccccac gatccctact
     1321 tcgagcgcca ttccggcggc cgagcgctaa ttcccgaagc cgacctgtgc gtcgcgcgcg
     1381 tagcgcttga cgtacaggac gcgagagagc acgaccgaga tgcgtccgtc ctcggtgtgg
     1441 agctgaagcg ttccctcgga gctcgccgcg agcgcccgct cgagcgcgtc cgctccctcc
     1501 gcggggacga acgcgcccag gatctgtccc ccgtcgaacg ccacttcgat tcggacacgc
     1561 tcagcgtcgg gcaacggaaa ccacctccgt gaggacgccg tggacggcgt cgggatggat
     1621 gaacgcgacc tcgaggccga agaggccggg tcgcggcgtc tcgtcgatca gctgcgcacc
     1681 ggccgcccgc agctcctgga gggcgccgcc gactgagtcg acctcgtacg caacgtggtg
     1741 cataccgggc ccgcgccggt cgaggtagcg gccgacgggc gtgtctgcgc cggtcggcgc
     1801 cagcagctcg atgcgggctt cacccacgag gacggagacg gcctcgaccc cttgcggcgg
     1861 cagcgtctgc cgatgctcga gcctcccgcc gaagagccgc tcgtaggttg cgagcgcctc
     1921 gtcgaggtcg gcgacggcca tgccgacgtg atggatgcgc ctgggatgca ccgcaggcga
     1981 ctctagtcaa cggatcactt ggcgttgccg gagagcgctc ggacggttcc gaccgtctca
     2041 gcggagtgcg actccggaat cacatcgacg agcatggctg caagctcgtt tcgctcgagc
     2101 gtctccttct cgagcagcgc gccggcgacc cggtcgagcg cgggccggtg cttctccagc
     2161 agccggcgcg cctcggtgta ggcgtggtcg gtgagccggg cctgctcgct gtcccgcaaa
     2221 cgcttcgtct cctccgagag cgcgtagttc tcggcgcgca tggtgcgcga cggggcaact
     2281 tcggacatgc cgaactcgaa caccatctgc cgcgcgatct cggtcacgcg ctcgaggtcg
     2341 ttggcggcgc cgttcgttat ccggccgaag acgacatgct ccgcggctcg accggagagg
     2401 aagatcttca tcaggtcgac gaactcctca cgggtgtgga gatagcggtc ttcggacggc
     2461 atgttcagcg tgtagccgag cgcctgtccg cgcgacacga tggtcacctt ctgcaccggg
     2521 atgagatcgc ccatcagatg ggccatcagg gcgtgccccg cctcgtggta ggcgaggatg
     2581 cgcttctcct tgtcggtgac gacgcggcgg gtctggagac cggccacgac ccgctccatc
     2641 gccgcttcga agtccgcctg ccggatgcgg tcttggtcgg agcggccggc gaagatggcg
     2701 gcctcgttcg caatgttcgc caggtcggcc cccgtcagcc cggctgtccg acgagcgatc
     2761 acgttcaggt cgacgtccgc cccgagcggc ttcgagcgcg tgtgcacgcg gaggatctct
     2821 tcgcgccccg cgagatcggg cggcgagacg agcacctgcc ggtcgaagcg cccgggccgg
     2881 agcaacgcgg ggtcgagatc ctgcagccgg ttggaagcac ccatgacgat cacctggtcg
     2941 cgaccgccga aaccgtcgag ctcgacgagc agctgattca gtgtctggtc gtgctcgcgg
     3001 ttgaacgatc cgccggagcg tgccgtcccg accgcgtcaa gctcgtcgat gaagacgatc
     3061 gagggagcgt tcttgcgggc ctcctcgaag agcttgcgga tgcgggcggc acccagcccc
     3121 gcgaacatct ccacgaacgc cgaggcgctc gacgagtaga agttggcacc ggatgcgctg
     3181 gcgacggcct tcgcgatcag agtcttacct gtcccgggag ggccgtacag gaggatgccc
     3241 ttgggcacct gcgcgccgag tcgctcgaat cgcttccggt ctcggaggaa gtcgacgacc
     3301 tcctgcagct cggccttcgc ctcgttgagc ccggcgatgt ccgcccacgc caccttctcg
     3361 ctcgaatccc gctcgacggg tgcgggcttg acccgcggca tgtactggac cgtgcgccag
     3421 agcaggtaga tgatgacgat gaagagaagg atcggcagcc aggtgagggc ccagtccgag
     3481 atgaggtcga cgaacgatgc ggcggcccag cccatagaac ccatgaaaag gatccccggc
     3541 gggccgtcaa ccgctcgttc gagtacgcac cgtgctttcg agcgcggcgg cgacctcgtg
     3601 ggaggtcgtg aagcgcactc cttcctggcg ccagagcgcc gtgcacgagg ccacgcgatc
     3661 gtccgaaagc ccttgcgccg catcctcggc gaacgcgact cgatatccgc gctccagcat
     3721 ccccatgatc gccgcgtggc tgcagacgtc ggtcgcgacg ccgaagacga tgatctcgct
     3781 cggcgcgagt gcccgcagga gcggctcggc gttgggattc gtgaatgcgc tgtagctctt
     3841 cttcagcagc aggatttcgc gccggccgac gacgagagcg ggtacgacgc ccggcgggta
     3901 cggaaggagc gagaagggca gcggatcgtc ctgctgcgtc gcctcgatct ttcgcgcacc
     3961 gccggtgccg cgcagacagt gcggcgggta ggtgttctcg aagtccggcg catctgagat
     4021 ctcgggatcg gtcagctcgt gatcgtctgc tgatgcgacg tgcacgagac ccgcggcgcg
     4081 tgccgcctgg acgagccggg ccatcgcgcc ccgcgtctcc tcggcgcccg gcacgtagag
     4141 cttgccgtgc ggctcgacga agtcgacctg ggtgtcgacg tcccagagaa caggttcaga
     4201 caacgcgctc cagccccggg tcggtgcgaa agcgtcggcc cactttcgca gacggcacgc
     4261 cgtccgtcag cacgatgtcg gccgcgtagt cgttctcccc gccgtcccgg atcctgtctt
     4321 ccggcagccc ggattcctct gccgggagcc gcctgccgac gcgagccgta gcctcggtca
     4381 gtcctcctcg agctcgaggc acgcggcgag cgcagcctcg gcgacctcga gttgctccgg
     4441 ggacggctcc tccgtcgaca accggctctg cagctcgtgg cccggtcgcg cgagcgcgcg
     4501 ggccacgggg tgctcgggat tccgggtcat ccaggcgaag acctcgaccg atgcgccgag
     4561 cgcgccgact gcggcggccg cacgcgcggc gggccgggtc cgccggggag tccgagccgc
     4621 gagggcaccg gccgcggccg acgacaggag cagtgggccg acaagatgcg agccgcaccg
     4681 ctcgtgctcc ttcgcagcgc gctcgccgtc ctgctcgtag gtcccgatcg agacgtgctc
     4741 tgctccgtgg tacgaagcca agtctccgcc gcgcagggcc agggccgccg gcgcgagcga
     4801 cgccacagcc gcgaggcctt cgcgagtggc ggtggagagc cgcgagcgac gggcggcgcc
     4861 tgcgacggca gcgccggcga cgaccgcagc cgcgacacgc ggtcgctccc acgggaagcg
     4921 agcttcgggc agacgccggc gaacgatcgg gagaagcgcg aacgcctcgg ccaaccgcag
     4981 cggtccccgt agcaacggtg cattgatgcg cgcgccgaag cggggcttgt accccgaagc
     5041 gattctgatc gccccgtcgg ggagcctcac cgcgcacgcc cacgccgtcg ggccgtgcac
     5101 caagaccccg ttcgcaagag ccattccgcc gagccggact ttctccatga gcagagggta
     5161 ccgtggctct cgatggacgc caccaccgtg ctggcgcgcc tcgccgcgct tcgcccggaa
     5221 cgcggtttcg tcttcgtcgg gatcggcggt cacggctgcg cgggcaagac cacgcttgcg
     5281 cgctccatac ccggagccac ctttgtcgcc acggacgagt tctgggacgg cagcgagttc
     5341 gacctgagac ggctccggcg cgaggtggtc gagccgttgc tcgagggacg cgaagcccgt
     5401 tttcactcgt tcgactgggc gaagcagaca ccacggcccg agcggcgtgc gatccggccg
     5461 gaggggatcg ttgtgatcga gggcgtctgc gccctgcacc ggatgttccg tgccgattac
     5521 gacctgcggg tgtgggtcga cacgccctac gagctcaggt tgaggcgggc gatcgaacgc
     5581 gacggcgaaa ggatgcgcaa gacctgggtc gatgtctgga tgcccggtga ggagcgctac
     5641 gtcgcaaagg acgacccggt cgccgccgca gacctcgtgg tcagcggcgc gtagctacac
     5701 ggccgcgtcg gcccggatgg ccgccagcaa ctcgccggcc gccggcccgt gatgcagggg
     5761 ctgtccgtcc agctcgacca ccggcaggag ctcccggacc gaggacgagg tgaacgcctc
     5821 ttctgccgcg agcagatctg cgagggcgaa gactccctcc tcgaccacat agccgatctg
     5881 gggcgccagg tcgagcacag actgtcgcgt cacccccgcg aggatgccga gctcgagcgc
     5941 tggagtgcgc agcgttctcc ccacccgcca ccagacgttc gtgacagggc cctcgagcac
     6001 ggtcgagtcg gcatcgac
//
LOCUS       DBNGNK010000156         3502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1817982,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000156 DBNGNK010000000
VERSION     DBNGNK010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1817982"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1594
                     /locus_tag="AC150V_04460"
     CDS             116..1594
                     /locus_tag="AC150V_04460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3762207.1"
                     /translation="MSGTGPRQLSHRSLWLQEALPAESPDLAARRRLAGSERADVCIL
                     GGGYCGLWTALFLKEREPSLDVVIVEADLCGGGASGRNGGFALSWWSKLATLVKLGGE
                     DEGRWLAEQSEASVRHIGEFAAEHGIDCDFVHAGWLWVASSGAQVDTWASTVEECTSR
                     GLDIFRPLTREEAQARGGSPRYLGGIFDPTAARVQPALLARGLRRVAIERGVRVFEES
                     PVVGVEGERPLVVRTPSGSVTVETVVSALNSWTVGLPQFKGLRRSIVTISSDIVATAP
                     DPERLASSGWTGGEIVSDSRLMVHYQRATRDGRIALGKGGGHLALAGVFGDEFHYDRR
                     LSERVTDSLHWLYPDWADVEITHAWGGPVDRTMTGIPFFGRAPGHERLFYGVGFSGNG
                     VAPTVTGGKILASLVLDADDRWGSCALTRGPEQLFPPEPIRSVGGVVVREAVRRKEQH
                     EDAGREPPWLLKRLAGFAPAGYFKVGTKKSHPASEGDSSVHG"
     gene            1657..2085
                     /locus_tag="AC150V_04465"
     CDS             1657..2085
                     /locus_tag="AC150V_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762208.1"
                     /translation="MTIALGIVALLLGLVIVVGQLVSVVDFPLAQWLGLQEKDEGTDP
                     LYRRLELNTARWDLLVLWTLPLAGVLMLVDSTWWAWMALVAGGIAVDTGGREIAKILG
                     LRWEGARVGTPSEARFVGGFLAVISAVGLALVGHSLVVLA"
     gene            2089..2529
                     /locus_tag="AC150V_04470"
     CDS             2089..2529
                     /locus_tag="AC150V_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762209.1"
                     /translation="MEVTALAWVVAVGGLLLIGLLGSLQLVAVVSPRGEWTIRNVYGG
                     DPSSTDPTAYFAFNQGYAWADPFFWAPLQVAGSVGVLLGERWGFLLALVASVPFWYSA
                     IPIFVWDRDMGFRRNTLTYWVVVWGMFPAYGLLEMTYCIVRLLG"
     gene            complement(2582..2809)
                     /locus_tag="AC150V_04475"
     CDS             complement(2582..2809)
                     /locus_tag="AC150V_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762210.1"
                     /translation="MALAVALGVRGDSPLPLEIAFLAVPVVCAFASAIAGGIAAIFAV
                     FHGERSALLLLPALVGAGALFFVIGELAVPH"
     gene            complement(3079..3375)
                     /locus_tag="AC150V_04480"
     CDS             complement(3079..3375)
                     /locus_tag="AC150V_04480"
                     /inference="COORDINATES: protein motif:HMM:NF018717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR3762211.1"
                     /translation="MPVYVVAQGHIDDRKMLDEYMSKVGETLTPELKILAIDETPEIV
                     EGPCEKPRTVVLEFPSKEDFRAWYDSPEYQAVMPLRTNSVDGWLVVADGFVPPE"
BASE COUNT          476 a         1159 c         1274 g          593 t
ORIGIN      
        1 cgcacatggg ctactactcg gaggactcgc tccgcgacct gcgcgcgtcg gtggcccgca
       61 acgccgcgtt ggtcttggcg ggcgagaagc cgctgtggga ggtcgtcccg tgaggatgtc
      121 cggtacaggg ccgcggcagc tgagccaccg ctcgctctgg ttgcaggagg ctctgccggc
      181 ggagtcgccc gaccttgccg cccggcggcg gctggccggg agcgagcgcg cggacgtgtg
      241 cattctcggc ggcggctact gcgggctctg gacggcgctc ttcctcaagg agcgggagcc
      301 gtcgctcgac gtcgtgatcg tcgaggcgga tctgtgcggc ggcggtgcga gcgggcgcaa
      361 cggcggcttc gcgctctcgt ggtggtcgaa gctcgccacg ctcgtcaagc tcggcggaga
      421 ggacgaggga cgctggttgg cggagcagtc cgaggcgtcg gtgcggcaca tcggcgagtt
      481 cgcggccgag cacggcatcg actgcgactt cgtccatgcc ggctggctct gggtcgcgtc
      541 gtcgggggcc caggtcgaca cgtgggcgtc cacggtggag gagtgcacga gccggggtct
      601 cgacatcttt cggccgctga cgcgggagga ggcgcaggcg cggggcgggt cgccgcggta
      661 cctgggcggc atcttcgacc cgaccgcggc gcgggtgcag ccggcgctgc tcgcccgcgg
      721 cctgcgccgg gtggcgatcg agcggggcgt tcgcgtcttc gaggagtcgc cggtcgtcgg
      781 cgtagaaggg gagcggccgc tcgtcgtccg gacaccctcg gggtccgtca ccgtcgagac
      841 ggtcgtcagc gcgctgaact cgtggactgt cgggctgccg cagttcaagg ggctgcggcg
      901 gagcatcgtg acgatctcga gcgacatcgt ggcgacggct ccggacccgg agcgactcgc
      961 gtcgagcggc tggaccgggg gcgagatcgt ctccgactcg cggctgatgg tgcactacca
     1021 gcgcgccacc cgggacggcc ggatcgcgct cggcaagggt ggcggccacc ttgcactcgc
     1081 aggcgtattc ggggacgagt tccattacga ccgtcgcttg tcggagcggg tcaccgacag
     1141 cctgcactgg ctctacccgg actgggccga cgtcgagatc acgcacgcgt ggggcgggcc
     1201 ggtcgaccgg acgatgaccg ggatcccctt ctttgggcgt gcgccgggac acgagcggct
     1261 cttctacggc gtcgggttct ccggcaacgg cgtcgccccg acggtgaccg gcgggaagat
     1321 cctcgcgtcg ctcgtgctgg acgcggatga ccgctggggg tcgtgcgcgc tcacgcgcgg
     1381 ccccgagcag ctcttccctc ccgagccgat ccgctccgtc ggcggcgtcg tcgtgcgcga
     1441 agcggtgagg cggaaggagc agcacgagga cgccggcagg gagccgccgt ggctgctgaa
     1501 gcggctcgcc ggcttcgcgc ccgccggcta cttcaaggtg gggacgaaga agtcccaccc
     1561 ggcttccgaa ggggattcct ccgtgcacgg ttaatccgag ggcgtttcag cggcgaccct
     1621 gatgcccggg atcgcgacga cgacgacgat agccccgtga cgatcgcgct cgggatcgtg
     1681 gcgcttctgc tcgggctggt catcgtggtg ggccagctcg tctccgtcgt ggacttcccg
     1741 ctcgcgcagt ggctcggcct acaggagaag gacgagggca cggatcccct ctaccgccgc
     1801 ctcgagctga acaccgcgcg ctgggatctg ctggtgctct ggacgctgcc tctcgcgggc
     1861 gtcctgatgc tcgtggattc aacgtggtgg gcgtggatgg cgctcgtcgc gggcggcatc
     1921 gccgtcgaca ccggtggtcg cgagatcgcc aagatcctcg gtctgcgatg ggaaggagcc
     1981 cgcgtcggca ctccgtccga ggcgcgcttc gtcgggggct ttctggctgt gatctccgcc
     2041 gtcgggctcg cgctcgtcgg ccactcgctc gtggttctag cctgagcgat ggaggtcacc
     2101 gcgctcgcct gggtcgtcgc cgtcggcggc ctgctgctca tcggcctgct cggctcgctc
     2161 cagctcgtcg ccgtcgtcag tccacgtgga gagtggacga tccggaacgt ctacggcggc
     2221 gatccgagca gcaccgatcc gacggcgtac ttcgccttca accagggcta tgcgtgggcg
     2281 gatcccttct tctgggcgcc gctgcaggtg gccggctcgg tcggggtgct cctcggcgag
     2341 cgctggggct ttctgctcgc cctcgtcgcg tcggtgccgt tctggtactc ggcgatcccg
     2401 atcttcgtct gggatcgaga catgggcttc cgccgtaaca cgctcacgta ctgggtcgtg
     2461 gtctggggca tgttccccgc gtacgggctg ctcgagatga cgtactgcat cgtccgcctg
     2521 ctcggttagg caaagagtcg cccgtttcgc aatgtgactc gaagtgcttc gacgaagccg
     2581 gtcagtgagg cacggcgagc tcgccgatca cgaagaacag agcgccagcg ccgacgagcg
     2641 ccggcaagag cagcaacgcc gatcgttccc cgtggaacac cgcgaagatc gcggcgatac
     2701 ctcccgcgat cgccgaggcg aacgcacaga cgacggggac ggcgaggaac gcgatctcga
     2761 gcgggagcgg cgagtcaccg cgaactccga gtgcgacggc gagagccatg acgccccaga
     2821 ggacgacggc ggcgccaagg agtgcgaagg aacgtctgcc gtacttcgtg gtcggaggcc
     2881 gtagcaccct tcggagcgtc cgtaagaatc cgccttcccg ctcgtgccac ggtgtcgcga
     2941 agtggcgcac cagccacctc cttccggata cagcattggc tcgggccggg gcaggcgcat
     3001 ccgcagattc tcgccgtctg cttgccgcaa agccgcgggg cgcagctcgc gcgtcccgcg
     3061 acctcgcgtc aactctctct actcgggcgg tacgaagccg tcggcgacca ccagccaccc
     3121 gtcgacggag ttcgtccgga gcggcatcac ggcttggtac tcgggcgagt cgtaccaggc
     3181 gcggaagtct tccttggacg gaaactcgag gaccacggtc ctcggcttct cgcacggccc
     3241 ctcgacgatc tctggcgtct cgtcgatggc gaggatcttc agctcgggcg tcagcgtctc
     3301 tccaaccttc gacatgtact cgtcgagcat cttccggtcg tcgatgtgtc cttgtgcgac
     3361 gacgtagacg ggcattggct cctcctcgtg ttcggtgatt tgggacgacg atcgttacac
     3421 acggcgcgat cggggacccg cgccgtgcca gtgcccgctc cgatcccgcg acatcgctac
     3481 ggttctcgcg gcagccgggc gt
//
LOCUS       DBNGNK010000157         6054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_312547,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000157 DBNGNK010000000
VERSION     DBNGNK010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_312547"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..666)
                     /locus_tag="AC150V_04485"
     CDS             complement(136..666)
                     /locus_tag="AC150V_04485"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3762212.1"
                     /translation="MTLAIGAEAPDFSLPGVDGSTRDLTDYSDARVLALVQSCNHCPY
                     VQAWEGRMSALQSEYGERGFRLVAVNSNDAASYPEDSFEAMTDRARTQGFTFDYLYDE
                     TQQFAREIGAERTPEVFLFDAERRLVYHGAIDDSRDEGGVSERYLQDAIEAVLAGETP
                     EVQDTPAVGCTVKWKR"
     gene            complement(663..968)
                     /locus_tag="AC150V_04490"
     CDS             complement(663..968)
                     /locus_tag="AC150V_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005195331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DF family (seleno)protein"
                     /protein_id="HIR3762213.1"
                     /translation="MIELLYWDGCPSHPEALELLREAAETTGRGDEIVLLEVRTQAEA
                     EELAFPGSPTIRVDGHDVDPVGAAAGRAMLACRIYRLPDGRPSPVPTREQIEEALRK"
     gene            1090..1263
                     /locus_tag="AC150V_04495"
     CDS             1090..1263
                     /locus_tag="AC150V_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762214.1"
                     /translation="MSTTIDKIRTLAQDGEWKTVDDLRNVSSYPEEWLKELRYEGFDV
                     REDEGRFRLRADD"
     gene            complement(1294..2070)
                     /locus_tag="AC150V_04500"
     CDS             complement(1294..2070)
                     /locus_tag="AC150V_04500"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR3762215.1"
                     /translation="MSARVCWITTAPVKGLALIERDEVELGESGIVENRRFHLIDAQG
                     LLLNGKRSGVLVRVAANWDAPADRLSLRLPDGEEVEADVELGETVTTHFYGRPVGGRV
                     VLGPFGEALSSVAGEPVRLVRPDESGAGVDRGFGGAVTILGAASLGRLAEAAGVEDVD
                     PRRFRMLFGIDGIAPHEEDTWIGRRVSLGDAVVEPTGNVGRCAVTTQNPDTGTPDLPT
                     LKALTSYRGVMETTEPLPFGIVGRVVTPGRVQVGDQARVS"
     gene            2126..2797
                     /gene="ung"
                     /locus_tag="AC150V_04505"
     CDS             2126..2797
                     /gene="ung"
                     /locus_tag="AC150V_04505"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3762216.1"
                     /translation="MEPRMHGSWLEVLGDEFEQPYMQELRRFLVVEVEGGRGFYPPAP
                     QVFNAMALTPFDEVRVVILGQDPYHGPGQAMGLCFSVPEGVRQPPSLQNIFRELHDDL
                     GLPVPTKGDLTPWARRGVLLLNAVLTVSPRQAGSHAGKGWEQFTDRAIAELSASGEGI
                     VLLLWGKYAQQKGAVVDMSRHHVLTAAHPSPYSASGFFGCRHFSRANELLASDGREPV
                     DWALP"
     gene            complement(2804..4177)
                     /locus_tag="AC150V_04510"
     CDS             complement(2804..4177)
                     /locus_tag="AC150V_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HIR3762217.1"
                     /translation="MAVRVVVVFAALGLLAAGCSADSSPNVETPTGVGRQFEEPPEAP
                     EGPLGADAQRAVGELIVSLEQGVDVAAIQAVGASGDARLAWVLSDLLRLIQVGSESEA
                     LVAAVERLTGVEFPAEEIEQGPWRTVTNQLIAWDLPEPPDYLGYKSAIYLIVEPRWAP
                     FFADSEADIDWRIVGWGGVLIDDRPLGDPNPCPLGCIPALDDPAVTNAAGGSWYPEDS
                     IVFGVVVGDEARAYPKNIMEVHEMVNDTLGGRRVGLPYCTLCGSAQAYFTDRVGDDDL
                     VLRTSGLLSRSNKVMYELGTMSIFDTFTGRAVSGPLRKAGVQLEELTVVTSTWAAWKE
                     AHPGTTIVARDGGIGREYPADPLRGRDDSGPIFPVGDIDSRLPAQEKVVGVIAADGTP
                     LAFPADAARAALRAGETVELEGVLLELDGDGLRVVGDSVDRLVSHEAFWFAWSQFHPE
                     TEVWERG"
     gene            4236..4670
                     /locus_tag="AC150V_04515"
     CDS             4236..4670
                     /locus_tag="AC150V_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762218.1"
                     /translation="MRPTRLEPILTAHPYLQVERPPLLWDVDADDEALIRALGEMISA
                     ALVRNGGVLAEITLNVSNVIVGPEAGESIPHGDYVALTIRARGDWSPEVTWQPGRTGS
                     GLLVGADLQAAAGAAGATYGYTRILRDGEGSVTVFLPRAAGG"
     gene            complement(4667..5437)
                     /locus_tag="AC150V_04520"
     CDS             complement(4667..5437)
                     /locus_tag="AC150V_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR3762219.1"
                     /translation="MSTQESGKRALKVEGLWKSFGAHDVLRGIDMEVDEGEVVVVFGR
                     SGSGKSTLLRCINFLEEPTVGTIEVDGIRLEIGHRTSAKREKIRQLRIRTGMVFQDFN
                     LFPHRSALDNVIEGPVTVLGKRRKEAEAVGRELLQTVGLPDKADHYPIQLSGGQQQRV
                     AIARALAMNPKVMLFDEPTSALDPELIGEVLAVMRRLATELRMTMLVVTHEMGFAREV
                     ASRMAFFHEGQIIEQGPPARVITDPQRPETKKFLEAVL"
     gene            complement(5434..>6054)
                     /locus_tag="AC150V_04525"
     CDS             complement(5434..>6054)
                     /locus_tag="AC150V_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR3762220.1"
                     /translation="HNAIAVGVTGFYTSFFRGTPLIVQMFLIYLALPQIGLALGDPWA
                     DILTLDVITAGVIALGLNYGAYMTEIFRAGIQSVGHGQAEAAEALGMTYFQKMKRIVL
                     PQAFRVIVPPTGNEFIAMMKDTALVSFLGTSVAQMEIFRRAQLVGKADFRNLEALIIA
                     ALMYWALTTLFQYFQLRLERRLAKGYVRGTAGVAVAGQRRAAEKKE"
BASE COUNT          949 a         2112 c         2042 g          951 t
ORIGIN      
        1 aggagggcca gccgcaaccc acgaaccgcg agcagcgcag gcgtctcgac cgaggcgccc
       61 aggggatgat cgtgcaacag cggcccggcg ccgccggtcg caagccgaag aagcgccgct
      121 aaccgcccgc gggcgctagc gcttccactt gacggtgcag ccgacggccg gtgtgtcctg
      181 cacttcgggc gtctcgcctg caaggactgc ttcgatcgcg tcctgcaggt agcgctcgga
      241 cacgccgccc tcatcgcggc tgtcgtcgat cgcgccgtga tagacgagcc ggcgctcggc
      301 gtcgaagagg aacacctcgg gtgtgcgctc ggcgccgatc tcacgtgcga actgctgcgt
      361 ctcgtcgtag aggtagtcga aggtaaagcc ctgcgtccga gcccgatcgg tcatcgcctc
      421 gaacgagtcc tccggatagc tcgcggcgtc gttcgagttg accgcgacga gccggaagcc
      481 gcgctcgccg tactccgact ggagtgcgct catccgcccc tcccaggcct gtacgtaggg
      541 gcagtggttg cacgactgga cgagggcaag gactcgggcg tcggaatagt cggtcaggtc
      601 ccgcgtcgag ccgtcgacgc cagggaggga gaaatcgggg gcctcggccc cgatcgcaag
      661 ggtcattttc gaagtgcctc ctcgatctgt tcacgggtgg gaacggggga cggacggccg
      721 tccggcagcc ggtagatacg acaggcgagc atcgcccgcc cggcggcggc accgaccggg
      781 tcgacgtcgt gcccgtcgac gcggatcgtc ggcgagcccg ggaaggccag ctcctctgct
      841 tcggcctgcg tccggacttc gagcagcacg atctcgtcgc cccgcccggt tgtctcggcg
      901 gcctcgcgca ggagctcgag cgcctccgga tgcgaggggc agccatccca gtagagcaac
      961 tcgatcaccc ccggagtctg cctgctcggg atgctgccgc gccgaaggtc ggttcccagt
     1021 tgaaaacctg accggttctt ctacactcgc ggctcgacca ggtgcacagg agactggagg
     1081 agggtgaggg tgagtaccac gatcgacaag attcgcacgc tcgcgcagga cggcgagtgg
     1141 aagaccgtgg acgaccttcg caacgtgagc tcgtatccgg aggagtggct gaaggaactc
     1201 cggtacgagg gattcgacgt gcgcgaggac gaaggacgct tccgcctgag agccgacgac
     1261 tagcgccggc gatcgtccaa cggcccgcgc cgctcagctg acgcgcgcct ggtctcccac
     1321 ctgcacgcgg ccgggcgtga cgacccggcc cacgatgccg aacggaagcg gctccgtcgt
     1381 ctccatgacg ccgcggtagg aggtcagagc cttcagggtt ggaaggtcgg gcgtccccgt
     1441 atcggggttc tgcgtcgtga ccgcacagcg gccgacgttg ccggtcggct cgacgacggc
     1501 gtcgcccagg ctgacccggc gcccgatcca cgtgtcttcc tcgtgcggcg cgatgccgtc
     1561 gatgccgaag agcatgcgga agcggcgtgg atcgacgtcc tcgacccccg ccgcctcggc
     1621 gagccgaccg agcgaggccg caccgagaat cgtcaccgcg cctccgaagc cgcggtcgac
     1681 tccggcgccg ctctcgtccg gtcggacgag gcggaccggc tcacctgcga cggaggagag
     1741 cgcctcgccg aacgggccga gcacgacccg ccctccgacg ggtcgcccgt agaagtgggt
     1801 cgtgacggtc tcacccagct ccacgtcggc ctcgacctcc tccccgtcgg gcagccgcag
     1861 gctgaggcga tccgccggtg cgtcccagtt cgcggccacg cgtacgagca caccgctgcg
     1921 cttgccgttg aggaggagcc cctgcgcatc gatcaggtga aagcgcctgt tctcgacgat
     1981 gccgctctcg ccgagctcca cttcatcgcg ctcgatcagc gcgagcccct tcacgggcgc
     2041 ggtcgtgatc cagcagaccc gggcgctcac gcgcgccatt ctcgcggacg aacggtgcct
     2101 ggcgaggcag taggctcgcc ccaacatgga gccgcgcatg cacgggagct ggctcgaggt
     2161 gctcggcgac gagttcgagc agccctacat gcaggagctt cgccgctttc tcgtcgtcga
     2221 ggtcgagggc gggcgtggct tctaccctcc cgcgccgcag gtgttcaacg ccatggccct
     2281 cacgccgttc gacgaggttc gcgtcgtcat cctcgggcag gacccgtacc acgggccggg
     2341 gcaggcgatg ggcctctgtt tctcggttcc ggagggcgtc cgacagccgc cctcgctgca
     2401 gaacatcttc cgtgagctgc acgacgacct cggtctgccg gtgccgacga aaggcgacct
     2461 cacaccctgg gcccgccggg gtgtgctgct gctcaacgcc gtgctgaccg tctccccgcg
     2521 ccaggccggg tcgcacgcgg gcaaggggtg ggagcagttc accgaccggg cgatcgccga
     2581 gctgtcggca agcggagagg gcatcgtgct cctgctctgg gggaagtacg cgcagcagaa
     2641 gggcgcggtg gtggacatgt cgcggcatca cgtcctcacg gcggcccacc cgtcgccata
     2701 ctcggcgtcg gggttcttcg gctgccgcca cttctcacgg gcgaacgagc tgctcgcctc
     2761 ggatggtcgc gagcccgtgg attgggcgct gccgtaggcg gctctagcct cgctcccaca
     2821 cctcggtctc gggatggaac tggctccacg cgaaccagaa ggcctcgtga ctgaccaagc
     2881 ggtcgactga gtcgcccacg acgcgcaggc cgtcaccgtc gagctcgagg agtacgcctt
     2941 cgagctcgac cgtctcgcct gcgcgtagcg ccgcccgcgc cgcatcggcc gggaaagcga
     3001 gcggcgttcc gtccgccgcg atcacgccga ccaccttctc ctgtgccggc agccgtgagt
     3061 cgatgtcgcc gaccgggaag atcggcccgc tgtcgtcgcg cccccggagc ggatcggcgg
     3121 ggtactcgcg gccgatgccg ccgtcgcggg ccacgatcgt cgtgccggga tgggcttcct
     3181 tccacgccgc ccaggtgctc gtgaccacgg tcagctcctc gagctgcacg ccggccttgc
     3241 gcagcgggcc cgacacggca cggccggtga aggtgtcgaa gatcgacatc gtgccgagct
     3301 cgtacatcac cttgttggag cgcgagagca atccgctcgt tcggagcacg agatcgtcgt
     3361 cgcccacccg gtccgtgaag tacgcctgtg cggagccgca gagcgtgcag tagggcaggc
     3421 cgacccgacg gccgccgagc gtgtcgttga ccatctcgtg cacctccatg atgttcttcg
     3481 ggtaggcgcg ggcctcgtcg ccgacgacga cgccgaagac aatggagtct tccgggtacc
     3541 acgatccgcc cgcagcgttc gtcacggccg gatcgtcgag cgcggggatg cagccgagcg
     3601 ggcacggatt cgggtcgccg agcggcctgt cgtcgatgag gacgccgccc cagccgacga
     3661 tccgccagtc gatgtcggcc tccgagtcgg cgaagaacgg cgcccagcga ggctccacga
     3721 tcaggtagat cgccgacttg tacccgaggt agtcgggcgg ctcgggaaga tcccacgcga
     3781 tgagctggtt cgtaaccgtc cgccatggcc cctgctcgat ctcctcggcg gggaactcga
     3841 cgccggtgag ccgctcgacg gcggcgacga gcgcttcgga ctcggacccg acctggatca
     3901 gccgcaacag gtcgctcagt acccaggcga gccgagcgtc accggaggcg ccgacggcct
     3961 ggatcgccgc gacgtcgacg ccttgctcga ggctcacgat cagctcgccg acggctcgct
     4021 gcgcgtcagc tccgagcggg ccctccggtg cctcgggcgg ttcttcgaac tgtcttccga
     4081 cccccgtcgg cgtctctacg ttcgggctcg agtccgccga gcatcccgcg gcgagcagtc
     4141 cgagcgcagc aaatacgacg acaactcgaa ccgccatgct gacacgctag cgacagcctt
     4201 cctcgcgaaa gactctgtta accgtacgcc aatcgatgcg ccccacccga ctcgagccga
     4261 tcctcaccgc ccatccgtac ctgcaggtgg agcgtccgcc gctgctgtgg gacgtggacg
     4321 cagacgacga ggccctcatc cgcgctctcg gagagatgat ctctgcagca ctcgttcgaa
     4381 acggcggggt gctcgcggag atcacgctca acgtctcgaa tgtcatcgtc ggcccggagg
     4441 cgggcgagtc gattcctcac ggtgactacg tcgcgctcac gattcgcgcg cggggcgact
     4501 ggagccccga ggtcacctgg cagcccggcc gcacgggttc cggcctactg gtcggcgccg
     4561 atctgcaggc ggcggccgga gccgcgggtg cgacctacgg ctacacgcgc atcctccgcg
     4621 acggcgaggg gtcggtcacc gtcttcctcc cccgggcggc gggcggctag agcactgctt
     4681 cgaggaactt cttcgtctcg ggccgctgcg ggtcggtgat cactcgcgcc ggcggcccct
     4741 gctcgatgat ctgcccctcg tgaaagaagg ccatacgcga ggcgacctcg cgcgcgaagc
     4801 ccatctcgtg cgtaacgacg agcattgtca tccggagctc ggtcgcgagc cgtcgcatca
     4861 ccgcgagcac ctcgccgatc agctcaggat cgagagccga ggtgggctcg tcgaagagca
     4921 tcactttcgg gttcatcgcc agcgctcgcg cgatcgccac gcgctgctgc tgaccgccgg
     4981 agagctgaat cggatagtgg tctgccttgt ccgggagccc gaccgtctgc aacagctcgc
     5041 ggccgactgc ctccgcttcc ttccgcctct tgccgagcac ggtgacgggc ccctcgatca
     5101 cgttgtcgag cgctgaacga tgcggaaaga ggttgaagtc ctggaacacc attccagtcc
     5161 ggatccgcag ctgccggatc ttctcccgct tggcactcgt gcggtggccg atctcgagcc
     5221 ggataccgtc gacctcgatc gtgccgacgg tcggctcctc caaaaagttg atgcagcgga
     5281 ggagggtgct cttgccggag ccggagcgcc cgaagacgac gaccacctcg ccctcgtcca
     5341 cctccatgtc gatgccgcgc aacacgtcat gcgctccgaa ggacttccag agcccttcga
     5401 ctttcagggc ccgctttccg gactcctgcg tgctcattcc ttcttctccg ccgcgcgtcg
     5461 ctggcccgcg accgccacgc cggcagtccc gcgcacatag cccttagcca gccggcgctc
     5521 gagccgcagc tggaagtact ggaagagcgt cgtcagcgcc cagtacatca gcgccgcgat
     5581 gatgagggcc tcgaggttgc ggaagtcggc cttgccgacg agctgggcgc gccggaagat
     5641 ctccatctgc gccaccgacg tcccgagaaa gctcacgagc gccgtgtcct tcatcatcgc
     5701 gatgaactcg ttcccggtcg gcgggacgat gacacggaac gcctgcggta ggacgatccg
     5761 tttcatcttc tggaagtacg tcatgccgag cgcctcggcg gcctcggcct gaccgtggcc
     5821 gaccgactgg atgccggctc ggaagatctc ggtcatgtag gcgccgtagt tgagcccgag
     5881 cgcgatgacg ccggccgtga tgacgtcgag cgtgaggatg tcggcccacg ggtcgccgag
     5941 cgcgagcccg atctgcggca gcgcgaggta gatcaggaac atctggacga tcagcggcgt
     6001 gccgcggaag aacgacgtgt agaagccggt gacgccgact gcgatcgcgt tgtg
//
LOCUS       DBNGNK010000158        13118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_27294,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000158 DBNGNK010000000
VERSION     DBNGNK010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_27294"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..834
                     /locus_tag="AC150V_04530"
     CDS             157..834
                     /locus_tag="AC150V_04530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762221.1"
                     /translation="MVDAAGSPAPVDKPAGYYAQERADLVDALPLPLGRVLDLGCGEG
                     GAARPLREAGAEWISGVELHGPAADRARDLYDEVLTGRAEDLLLELSAPFDTILAYDI
                     LEHLPDPDSLVRGLSEIASPGARLHVSVPNARHYSLVRDLVLRGTYGYEAAGHRDATH
                     LRWFTRRDLEALLAGAGWEPVPGSHPALRGRRARIHRLTRGRLAEFTFWQWVVLAYRR
                     DTAPSRS"
     gene            complement(801..1805)
                     /locus_tag="AC150V_04535"
     CDS             complement(801..1805)
                     /locus_tag="AC150V_04535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3762222.1"
                     /translation="MDEATVVIPTYNGYELLSGALTALQSQTAPVSIIVVDNGSSDGT
                     RTLVAERFPDVRLLHNDRNLGFGRAVNQGARAASTASGALVLVNNDCVCEPEFVERIC
                     APLAHPGIGMAAGVLLQAEEPGRIDSAGIELDSTLRSYDYLWNRPVEALEGALDPVGP
                     CGGAAAYRFDAFREVGGFDEAFFAYWEDVDLALRLRLAGWGCALAPGARAVHEHGQTV
                     GAGSPLARRLDAFGRGYVLGRYRVAERGLSDRATVALVDWPALMTHALLRRELSPLWA
                     RLRGLSRGRARNGLQAPLESVTVSVGEAVSRQWRFLALRLRGRAPRHFYEREGAVSRR
                     "
     gene            1874..3340
                     /locus_tag="AC150V_04540"
     CDS             1874..3340
                     /locus_tag="AC150V_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR3762223.1"
                     /translation="MSAGTPPVDVLRTPEAGGKAIRGGTLRGGGYAIGLLLGAVTSVF
                     LLRGLGVEDFGRYGTVAALLGIVSTLTDAGLTAVGARELALRPPGPERERLLQLLLGL
                     RVLLSVVAVLAAVAFAAIVGYDEVMIWGTLIAGFGVLLVNTQATAMMPLSVELRLGAV
                     TAFEVLRQAITLAGVAFLAIVGASLLPYFLVQVVAGVIVLAVTPAVVGGLRGLRPRLG
                     RTEAAVLLRDALPVGVAIAMNVIYLRLLVILVSLLEGERETGEYATAFRIFELLIGVP
                     ALVIAVVLPVLAVAADDRPRLRYGVQRTTEVAFVASVGLALATTAFAAPAVDLLFGPQ
                     FAGSVPMLQLQVWALVPLFLGSVASLALLSLRRQRSIAVANVAAVVVVLAVGLTLIPI
                     YGGMGASAAGVATELVLAVLLFAFLLRADRSVQPHFGFAWRSLVALAAGLAPLFVPGL
                     GDWVAAAVSVTAFLLAALLFRAVPREVFDALFRRGGDA"
     gene            3337..4440
                     /locus_tag="AC150V_04545"
     CDS             3337..4440
                     /locus_tag="AC150V_04545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762224.1"
                     /translation="MSRPRVVLLRGHSANPWELRTWELLVDRFDIVVGVTGSNLHDVS
                     GVALPVTRVRALRDRLPPGRLGDLAALGAGDRYLGLEDALRGADIVHSAELGVWFAAQ
                     PATLRTKLGHRLVLTVWETIPFRDTFRAFRGRRQRRVTLENADLFLAATERARRCLLL
                     EGADDNRIVVAPPGIDVERFRAHAPAGDGGHLIVSPGRLVWEKGHFDVLRALAVVGGE
                     HRLLIVGAGPERARLERYAGELGLAGRVEIRAVPYDQMPAVFASASAVVLASLPIPFW
                     EEQFGMVLAEAMAAGAPVLASASGAIPEVLEGSGAPLFEPGDWVGLARLLADGPLARA
                     PGERVAYAAAVIERYSTTAAAERLAGAYERVLS"
     gene            complement(4463..5524)
                     /locus_tag="AC150V_04550"
     CDS             complement(4463..5524)
                     /locus_tag="AC150V_04550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762225.1"
                     /translation="MRVGLAMLTLVPGISGGSETYARELCRGLARSGGLDYTAYVPRI
                     AADAGDGLPTEVVDEYRAATSRAGRLAALALASARPGPLRARLGGCDVVHFPLTVPLP
                     VMRSPTVVTLHDLQHVDLAPLFSRAERAYRRRTYDGAAKRADAVIVVSRFVKERVVAT
                     LGVDAARVHVIHSGVDRDRFTPADEPRERFLFYPARPWPHKNHERLLEALARLRTEDP
                     ELRLVLTGAGTEQLSGIPGVDARGTVSAEELVALYRRAACLVFPSLYEGFGLPPLEAM
                     ACGCPVAAAAAGALPEVCGKAAVLFDPREPEAIAAGVGEALSRADELRALGLERAAGF
                     SWDETARGHDAVYRLLGEA"
     gene            5586..5999
                     /locus_tag="AC150V_04555"
     CDS             5586..5999
                     /locus_tag="AC150V_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762226.1"
                     /translation="MPHDGAENGESTGFPAASEVDVEALLESLREEVRKSGADPGGAP
                     GGAADRRAAREEAERLWPVSADRSLRLRPGFRGGLGVPAKAVLRKLMRWYVEPLAYDQ
                     RSFNAAVLRLLDDLQDQVARLEAELEAERAKNGPK"
     gene            5996..7021
                     /locus_tag="AC150V_04560"
     CDS             5996..7021
                     /locus_tag="AC150V_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762227.1"
                     /translation="MNIAVCRPQVPFAHGGAEIMTDALVAELRARGHDADVVSIPFKW
                     YPGVRVLTQAFLWRLVDLTETSGKSIDLVVATKFPSYVVQHPEKRVWLIHQFRQAYEL
                     DRTDMNQFGESARDRAVRRKIHQLDRLALGEAKRLFATSRNVAGRLERSTGLVAEVLP
                     HPPQELDYRCDGYGDFVLSASRLDRAKRIDLLLEAAVLDPALKVVIVSDGPDRSRLEG
                     LARKRRLDGRVRFAGRVDSAELAELYAGCLATYYAPVDEDFGMGPYESFLAQKPVITT
                     TDSGGPLDVVHDRKTGIVVTPEAAEIAKATAWLREHLDDAAAYGRAGKAIADEVTWDR
                     AIARLLS"
     gene            7018..8304
                     /locus_tag="AC150V_04565"
     CDS             7018..8304
                     /locus_tag="AC150V_04565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762228.1"
                     /translation="MKVPYGRRSRGRRPGFLKVAYFSPMPPERSGIADYSALLVPALR
                     ERVDLLVPRRGRRRPSGRAHVSVYHIGNNPDAHAWILDVLRRKPGLVVLHDFVLHHLV
                     AGVTIGRRDGHGYLNAMERENGVVGRLLGHGVLDKRIPPLWENRPDEFPLAGEVLDLA
                     TGLIVHSQYVRDRARAAGYEGPISVVPHPAWPVPRVDPVQPASGLVVGAFGNVNASKR
                     VPQLLAAFARVRRTHPDATLLLVGATSPGFDLDRRLQRHGIDAAGLVREEWVSEERLW
                     GLMLGADIHVSLRAPTMGETSGTAVRALALGKPLVVSETGWFAELPGEVALKVPAGDE
                     EVETLAAGLELLASRPDLRAAMGSAARELAETEHELGHVADLYVRACEEAAGGPKVAE
                     AVLGEVASAAAAVGIEPGGEAASDIARRLAEVELHE"
     gene            8297..10390
                     /locus_tag="AC150V_04570"
     CDS             8297..10390
                     /locus_tag="AC150V_04570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3762229.1"
                     /translation="MSKAFRAVPAWGWLSAIVIASFGVRAWLARGMPAPFIMVDELIY
                     SDLARSIADAGRLLVRDVPASGFGVVYPLLLSPVYLIYDSLTDAYAAAKTVNSLLMSL
                     AAIPAYLLARRVASMWLALAAALLAVSVPSLVYTATVMTENVFYPIFLTAAYGLVLVL
                     ERPSWRREVVFYAFVAVAVLTRVQAVALVAAALTAPLLLAAFERGGLRTTLRPFRRLY
                     AVLLGLVALVVVVQAARGRALSDLVGAYSVVGDRDYDVGQVLRFALWHLEELSLYLGI
                     APFAALIILVGLGRSRTRPTKAFLAATLALTAWFLVVVAAFASEFANRIQERNLFVVG
                     ALFLVALVAWVEQRAPRPLALTIGALALAGGLPLLFPYGRFIETAAVSDTLALLPIWT
                     AFGSLLLDSIVFTVAVGVVLMCLLFAFVPARYAVLVPLAVLVWFAIMFKPVWSGPHGF
                     KQAGIGALFQGIRGVERGWIDRAVPAGEEVAVVWTGRSDRFTVNENEFFNRRVGPVYY
                     VGGPTPGGLAETEVTIGEDDGVIRLLDGSPVKATYVLVDDSIAPDGEPIARDELLGMT
                     LWRVKGDLVSTTTVEGLYPNDTWSGPQVVYTRQRCEGGTLSVSLSSDPTLFVGKRTTV
                     VGRSEEGVEEFVNFGASETTELSVPLVSRDDRCVVVFDISPTLVPAEVLPGNSDERDL
                     GAHFNSFVYEPPPSR"
     gene            10387..11466
                     /locus_tag="AC150V_04575"
     CDS             10387..11466
                     /locus_tag="AC150V_04575"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762230.1"
                     /translation="MRIAFDVSPLSHPRTGIGQYLRGSLAGLAQAAAAEGGHEIIAFA
                     PTSPRGRRAIPEALAGIPVQLRLRLLPFSHAFRMAWSRRGRPPVERFLGRVDALHFSD
                     WMYPPQEGGVRATTIHDLVPLRFPEWTTERTREMHGEKYENAAATCDVIFVNSAYTAA
                     DVRSRLGVPENRIRIARPGLSERFNPGGEVADLGRPYLLGLGNLEPRKNLVQLVAARR
                     LLPDAPALALTSGGEWGDGVRLDDPAILKLGFVDDEEVPRLLRGADVFVYPSLFEGFG
                     MPIVEAMACGTPVVASSHPSMDEACGDAAIRVDPEDAEAIAEGIQEARERRVELVERG
                     LEHAAGFTWRRVGDVFLRGYREAAA"
     gene            11463..12452
                     /locus_tag="AC150V_04580"
     CDS             11463..12452
                     /locus_tag="AC150V_04580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762231.1"
                     /translation="MRVGLDVAPLVQTQAGTARWVRGLLGALERHRDVEPVPITWGGE
                     GRVTAAVRDVVWYPLLLPRRARTGRFDLVHTTIYRGPLRSGVPVVVTVHDLAVLRYPD
                     AFPAWTRLYGRTALRRTLRAARRVVAVSEFSRRETAALAGVPADRIDVVPNGIEPVFD
                     AAGPAAEGDYVLAVGTIEPRKNLGRAIEAARLAGASLRVVGARGWGNVQAEGPGVEWL
                     GRVSDEELACLYRGARSLVYPSLYEGFGIPVAEAMACGCPVVTSAGSAMADVAGGAAI
                     LVDPHDVGAIAAGIDEADRRRDVLVPLGLERARAFTWDRAGQAAVAAYRRALA"
     gene            12449..>13118
                     /locus_tag="AC150V_04585"
     CDS             12449..>13118
                     /locus_tag="AC150V_04585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3762232.1"
                     /translation="MSRPLVVVDADVLGRRRTGDETYMRNLLRELPAPAGEAGLRLAA
                     VTRRPELVPQGIEPLPLSIAPQELRMAWSLPRLLRRVDAALVHTQYACPPRSPCPAVV
                     TVHDLSFERDPSLMSAKDRLVFRVIVPRSVRRAARVLTVSDRTKLDLEDLYGVPPEHI
                     VVTPNGVDPVFTPEPPPFVDGNGSAPDAYILSVGAIQRRKNQEAALRAARQAGIELGV
                     VGPEK"
BASE COUNT         1680 a         4654 c         4645 g         2139 t
ORIGIN      
        1 agcgcgtcgg tcaggtgcga tcccatgaag ccgtccgcgc ccgtcaccag cgctgtgcgc
       61 ccctcgaagt cgtcggcgaa ctccgcccgc aggccggcga ttcccgcctc gtcccagatc
      121 ggcccggcat ctgccacgac ggcgtaggct agcgggatgg tggacgcggc ggggagtccg
      181 gcgccggtcg acaagcccgc cggctactac gcgcaggagc gcgcggacct cgtcgacgcc
      241 ctgcccctgc cgctgggccg cgtgctcgac ctcggctgtg gcgaaggcgg cgccgcccgg
      301 ccgctgcgcg aggcgggcgc agagtggatc tcgggggtcg agctgcacgg gcccgcagcg
      361 gatcgggcgc gcgacctgta tgacgaggtg ctcaccgggc gcgccgaaga cctgctcctg
      421 gagctttccg cgccgttcga cacgatcctc gcctacgaca tcctggagca cctgcccgac
      481 ccggatagcc tcgtgcgcgg gctgagtgag atcgcctcgc cgggcgcgcg cctccatgtc
      541 tcggtgccca acgcacgcca ctactcgctc gtgcgcgacc tcgtcctccg cggcacgtac
      601 ggatacgagg cagccggtca ccgcgatgca acgcacctgc ggtggttcac ccgccgcgac
      661 ctcgaggcgc tgctcgccgg tgccgggtgg gagcccgttc ccggctcgca cccggcgctc
      721 cgcggtcggc gggcgcgcat tcaccgcctc acgcgcggcc gcctcgcgga gttcacgttc
      781 tggcagtggg tcgtgcttgc ctatcggcgc gacacagcgc cctcgcgctc gtagaaatga
      841 cgtggggctc gtccgcgcag ccgaagagcg agaaagcgcc actgtcgcga gaccgcctcg
      901 ccgaccgaca cggtcacgct ctcgaggggc gcctgtaacc cgttccgtgc tctgccgcgt
      961 gagagccccc gcaggcgtgc ccacagcggc gagagctcgc gccgaaggag agcgtgggtc
     1021 atgagcgcag gccagtcgac gagcgcgacc gtggcgcggt cggacagccc gcgctcggcg
     1081 acccggtagc gcccgagcac gtagccgcgg ccgaaggcgt cgaggcgtcg cgcgagcgga
     1141 gagccggcgc cgacggtctg gccgtgctcg tgcaccgcac gtgcccccgg agcgagggcg
     1201 cagccccatc cggcgaggcg gaggcgaagc gcgaggtcga cgtcctccca gtaggcgaag
     1261 aacgcctcgt cgaaaccgcc gacttcccgg aaggcgtcga agcgatacgc ggcggccccg
     1321 ccgcagggcc cgaccgggtc gagcgcgccc tcgagggctt cgacgggcct gttccagaga
     1381 tagtcgtacg agcggagcgt cgagtcgagc tcgatccccg ccgagtcgat gcgcccgggc
     1441 tcctcggcct ggagcagtac gcccgccgcc atgccgattc cagggtgcgc cagcggtgcg
     1501 cagatgcgct cgacgaactc gggctcgcag acgcagtcgt tgttgacgag cacgagggcc
     1561 ccactcgccg tggacgcggc gcgggcgccc tggttgacgg cccgaccgaa gccaagattc
     1621 cgatcgttgt gaaggagcct gacgtccggg aatcgctccg cgacgagcgt ccgcgtcccg
     1681 tccgacgagc cgttgtcgac gacgatgatc gacacgggcg ccgtctgcga ctggagcgcc
     1741 gtgagcgcgc ccgagagcag ctcgtagccg ttgtacgtgg ggatgacgac ggtcgcctcg
     1801 tccattgcgc gaaggcaccg agcctacccg ccggcgggcg gcgtccggcc tgcgcctcgg
     1861 ttacggtccg gcggtgagcg caggcacgcc gccggtcgac gtcctccgca cgccagaggc
     1921 gggaggaaag gcgattcgcg gcggcacgct gcgtggtggt gggtacgcga tcggcctcct
     1981 cctcggcgcg gtcacatcgg tgtttctgct ccgcggtctg ggcgtcgagg acttcgggcg
     2041 ctacggaacc gtggccgctt tgctcggcat cgtctcgaca ctcaccgacg cggggctcac
     2101 cgccgtcgga gcgcgtgagc tcgcactccg accaccgggg ccggagcgcg agcggctgct
     2161 ccagctcctg ctcgggctgc gcgttctcct gagcgtcgtc gcggtgctcg cggccgtcgc
     2221 tttcgccgcg atcgtcggct acgacgaggt gatgatctgg ggaacgttga tcgccggctt
     2281 cggcgtcctt ctcgtcaaca cccaggcgac cgcgatgatg cccctctcgg tcgagttgcg
     2341 gctcggcgcc gtcaccgcgt tcgaggttct ccggcaggcg atcacgctgg ctggtgtcgc
     2401 ctttctcgcg atcgtcggcg cctccctgct gccgtacttc ctggtgcagg tcgtggcagg
     2461 agtgatcgtg ctcgcggtca cccctgccgt cgtcggggga ctccgagggc tgcgaccgcg
     2521 gctgggacgt acggaggccg cggtactgct tcgagatgcc ctcccggttg gggtcgcgat
     2581 cgcgatgaac gtgatctacc tgcgcctgct ggtgatcctc gtctcgttgc tcgagggcga
     2641 gcgggaaacc ggcgagtacg cgaccgcgtt ccgaatcttc gagctgctga tcggggttcc
     2701 ggcactggtg attgccgtcg tgctcccggt gctcgcggtc gccgcggacg atcgtccgcg
     2761 gctccggtac ggcgtccagc ggacgaccga agtcgcgttc gtggcgtcgg tcgggctcgc
     2821 cctcgcgacg acggcgttcg ccgcgcccgc cgtcgacctt ctgttcggcc cgcagttcgc
     2881 gggctcggtt ccgatgttgc agcttcaggt ctgggctctc gtgccgctct tcctcggctc
     2941 cgtggcctcg ctggccctgt tgtcccttcg ccgtcagcgc tcgatcgcgg tcgccaacgt
     3001 cgccgcggtc gtcgtggtcc tggccgtcgg gctcacgctg attcccatct acgggggcat
     3061 gggggcttcg gccgcgggtg tggccaccga gctcgtcctg gccgtgcttc tctttgcgtt
     3121 cctcctgcgc gcagatcgct ccgtccagcc gcacttcggc ttcgcctggc ggtcactcgt
     3181 cgcactcgcg gccgggctcg cgcccctctt cgtcccgggc ctaggcgact gggtggctgc
     3241 agcagtgtcg gtcaccgcct tcctcctcgc cgcgctcctg ttccgcgccg ttccgcgcga
     3301 ggtgttcgac gccctcttcc gccgcggcgg cgacgcgtga gccgcccgcg cgtcgtcctg
     3361 ctgcgcggcc acagcgcgaa cccgtgggag ctccgcacgt gggagctgct ggtcgaccgg
     3421 ttcgacatcg tcgtcggggt cacggggagc aacctccatg acgtctcggg cgtcgcgctt
     3481 ccggtcacgc gcgttcgcgc gcttcgcgac cgtctgccgc ccggacgcct cggtgatctg
     3541 gcggccctcg gggccggtga tcgctacctg ggcctcgagg atgcgctccg cggcgccgac
     3601 atcgttcaca gcgccgagct gggcgtctgg ttcgccgcgc aaccggcgac gctgcgtacg
     3661 aagctcggcc atcggctcgt cctgacggtc tgggagacga tcccgttccg cgacacgttt
     3721 cgggcgttcc gcgggcggcg ccagcgtcgc gtgaccctcg agaacgccga tctcttcctt
     3781 gcggcgaccg agcgcgcccg ccgctgtctc ctcctcgagg gcgccgacga caatcgaatc
     3841 gtcgtcgcgc caccggggat cgacgtcgag cgttttcggg ctcacgcacc ggccggggac
     3901 ggcgggcacc tgatcgtctc ccctggccgg ctcgtctggg agaaggggca tttcgacgtt
     3961 ctgcgtgcgc tcgccgtcgt cggcggtgag catcgcctgc tgatcgttgg ggccggaccc
     4021 gagcgggcgc ggctcgagcg gtacgcaggc gagctcggat tggcgggtcg tgtcgagatc
     4081 cgtgccgtcc cgtacgacca gatgccagcc gtcttcgcgt cggcttcggc cgtcgtcctc
     4141 gcgagcctgc cgattccgtt ctgggaggag cagttcggca tggtgctcgc cgaggcgatg
     4201 gccgccgggg caccggtgct cgccagcgcc tcgggagcga ttcccgaggt gctcgaaggg
     4261 tccggcgcgc ctctcttcga gccaggcgat tgggtcggcc tcgcccgact gctcgccgac
     4321 gggccgctgg cgcgggctcc gggcgagcgg gtggcgtatg ccgcggctgt catcgagcga
     4381 tactcgacga cagccgcggc cgagcgcctg gccggcgcct acgagcgtgt cctgagctag
     4441 taacagtctg ttgctagccg gattacgctt ctccgaggag ccggtagacg gcgtcgtggc
     4501 cacgggcggt ctcgtcccag gagaatcccg cggcacgctc gagcccgagc gcccgcagct
     4561 cgtcggcccg cgacaacgcc tccccgacac ctgcggcgat cgcctcgggc tccctcggat
     4621 cgaagagcac ggcagccttg ccgcacacct ccggcagcgc gccggcggcg gccgcggcga
     4681 ccgggcaccc acacgccatg gcttccagcg gcggcagtcc gaagccctcg tagaggctgg
     4741 ggaagacgag gcatgccgcg cgccggtaga gagcgacgag ctcttccgcc gagacggtgc
     4801 ctcgggcgtc gactccgggg atcccgctca gctgctcggt tcccgctccc gtcaggacga
     4861 ggcgtagctc cggatcctcg gtacggaggc gggcgagtgc ctcgagcagc cgctcgtggt
     4921 tcttgtgggg ccacggtcgg gccggataga agaggaagcg ctcgcgaggc tcgtccgcgg
     4981 gcgtgaaccg gtcacggtcg acgccggagt ggatcacgtg cacgcgggcc gcgtcgacgc
     5041 cgagcgtcgc gacgacgcgc tccttgacga atcggctcac gacgatcacc gcgtccgcgc
     5101 gtttggcagc gccgtcgtac gttcgtcgac ggtaggcgcg ctcggcccgc gagaagagcg
     5161 gagcaagatc gacgtgctgg agatcgtgga gcgtgacgac cgtcggcgag cgcatcaccg
     5221 gcaggggcac cgtcagtggg aagtgaacga cgtcgcagcc tccgagtcgc gcccgcagcg
     5281 ggcccggtcg agccgaggcg agcgccagcg cggcaagccg gccggccctg ctggtcgcgg
     5341 cccggtactc gtcgacgacc tccgtcggga gcccgtctcc ggcgtcggct gcgattctcg
     5401 gcacgtaggc cgtgtagtcg agaccgcccg aacgcgccaa cccgcggcag agctcacgcg
     5461 cgtacgtctc gctcccgccc gagataccgg gaacgagcgt gagcatggcg aggccgacgc
     5521 gcaccggggg cgcatcgtat cgcccagacg ggaggcgtat cgtgcgccct cggtagaatg
     5581 ccgcgatgcc gcacgacggc gcggaaaacg gcgaatctac cgggtttccg gccgcctcag
     5641 aggtcgacgt cgaagccctt ctcgagtcgc tccgtgagga ggttcggaag tccggtgccg
     5701 atccgggtgg ggctcccggc ggcgcagccg accgccgtgc cgcccgggaa gaggctgagc
     5761 ggctctggcc cgtctcggcc gaccgctcgc tgcgattgcg ccccggattc cgcggcggtc
     5821 tcggcgttcc ggcgaaggcc gtgctgagaa agctgatgcg ctggtacgtc gagcctctcg
     5881 cctacgacca gcgctcgttc aacgctgccg tgctacggct gctcgacgac ctgcaggatc
     5941 aggtcgcacg gctcgaggcc gaactggagg ccgagcgcgc gaagaacggc ccgaagtgaa
     6001 catcgcggtc tgccggcccc aggtgccgtt cgcgcacggc ggcgccgaga tcatgacgga
     6061 cgctcttgtc gccgagctcc gggctcgggg ccacgacgcc gacgtcgtgt cgatcccgtt
     6121 caagtggtat ccgggcgtcc gtgttctcac gcaggcattc ctgtggcgcc tggtcgacct
     6181 caccgagacg tccgggaagt cgatcgacct cgtcgtcgcg accaagttcc cctcttacgt
     6241 ggtgcagcat ccggagaagc gggtgtggct gatccaccag ttccgccagg cctacgagct
     6301 ggatcgaacg gacatgaatc agttcggcga gtccgccagg gacagggccg ttcggcgcaa
     6361 gatccaccag ctcgaccggc tcgcactcgg ggaggcaaag cgcctgtttg ccacctcccg
     6421 caacgtcgcg ggccggctcg agcggtcgac ggggctcgtc gcggaagtgt tgcctcatcc
     6481 accgcaggag ctcgactatc gctgcgacgg ctacggcgac ttcgtcctct ccgccagccg
     6541 gctcgatcgc gccaagcgca tcgacctgct gctcgaggcc gccgtgctcg atccggctct
     6601 caaagtcgtg atcgtctccg acggccccga ccgctcccgg ctcgagggcc tcgcccgcaa
     6661 gcggcgtctc gacggacgcg tccggttcgc cggtcgggtc gactcggcgg agctggccga
     6721 gttgtacgcg ggctgcctcg ccacgtacta cgcgcccgtc gacgaggact tcggcatggg
     6781 cccgtacgag tcgttcctcg cccagaagcc ggtgatcacg acgaccgact ccggcggacc
     6841 gctggacgtc gtgcacgatc gcaagacggg gatcgtcgtc acgcccgagg cggctgagat
     6901 cgcgaaggcg acggcgtggc tccgcgagca tctcgacgac gcggcggcgt acgggcgcgc
     6961 gggcaaggcc atcgccgacg aagtgacctg ggatcgggcg atcgcgaggc tgctctcgtg
     7021 aaggtcccgt acggccggcg gagcagaggg cgacggccag gcttcctgaa ggtcgcctat
     7081 ttcagcccga tgccgccgga gcggtccggc atcgccgact actccgccct gctcgtcccc
     7141 gcgctgcggg agcgcgtcga cctgctcgtc ccacgtcggg gtcgacgccg gccctccggg
     7201 cgcgctcacg tctccgtcta ccacatcggc aacaaccccg acgcgcacgc ctggatcctc
     7261 gacgtgctcc gccggaagcc cgggctcgtc gtgctccacg acttcgtcct ccaccatctg
     7321 gtcgccggcg tgaccatcgg gcgtcgagac ggtcacggct acctgaacgc gatggagcgg
     7381 gagaacggag tcgtgggccg cctgctcggc cacggcgtgc tcgacaagcg gatcccgccg
     7441 ctctgggaga accggccgga cgagttcccc ctggcgggag aggtactcga tctcgcaacg
     7501 ggtctgatcg tccactcaca gtacgtccgc gaccgcgcac gggcggcagg atacgaaggc
     7561 ccgatctcgg tcgttccgca tcccgcgtgg ccggtgccgc gcgtcgaccc cgtccagccc
     7621 gcatcgggtc tcgtggtcgg agcgttcggg aacgtcaacg ccagcaagcg cgtgccgcag
     7681 ttgctggccg cgttcgcgcg cgtccggcgc acgcatccgg atgcgacgct cctgctcgtc
     7741 ggggcgacct cgcccggctt cgacctcgac cggcggctgc agcgccacgg catcgacgcc
     7801 gccgggctcg tgcgagaaga atgggtctcg gaggagcgac tgtggggctt gatgctcggc
     7861 gccgacatcc atgtgagcct ccgggcgccg acgatgggcg agacctccgg cacggcagtc
     7921 cgggcgctcg ctctcggcaa gcctctcgtc gtcagtgaga cgggctggtt cgcagagctg
     7981 cccggggagg tcgccctcaa ggtgccggcc ggcgacgagg aggtcgagac gctcgcggcg
     8041 ggactcgagc tgctggcgtc gcgccccgat ctccgcgcgg cgatgggctc ggcagcgcgc
     8101 gagcttgcgg agaccgagca cgagctcggg cacgttgccg atctctacgt tcgcgcgtgc
     8161 gaggaggcgg caggcgggcc gaaggtagcg gaggcggtgc tgggcgaggt cgcgtctgcg
     8221 gcggcggctg tggggatcga gcccggcggg gaggccgcga gcgatatcgc gcgccgcctc
     8281 gccgaggtcg agctgcatga gtaaggcgtt tcgcgcagtg ccggcctggg gctggctcag
     8341 cgcgatcgtc atcgcctctt tcggagtccg tgcctggctc gcgcgcggca tgcccgcgcc
     8401 gttcatcatg gtcgacgagc tcatctactc cgacctggcg cgcagcatcg ccgacgcggg
     8461 caggctcctc gtgcgagacg tgccggcaag cgggttcggg gtcgtctacc cgcttctgct
     8521 gagcccggtc tacctgatct acgacagcct caccgacgcg tacgcggctg ccaagaccgt
     8581 gaactcgctg ttgatgtcgc tcgccgcgat tccggcgtat ctcctcgccc ggcgcgtcgc
     8641 gtcgatgtgg ctggcattgg cggccgctct gctggccgtc tccgtgccct cgctcgtcta
     8701 cacggcgacg gtgatgacgg agaacgtctt ctacccgatc ttcctgacgg ccgcctacgg
     8761 gctcgtcctc gtgctggagc gtcccagctg gcggcgcgag gtcgtcttct acgcgttcgt
     8821 cgccgtcgca gttctgaccc gcgtgcaggc cgtcgcactc gtggccgccg cgctgacggc
     8881 acccctgctg ctcgcggcgt tcgaacgtgg cggcctgcgg acgacgctgc ggccctttcg
     8941 ccggctgtac gccgtgctgc tcggactcgt cgcgctcgtc gtcgtcgtcc aggcggcccg
     9001 cggccgtgct ctctcggatc tggtcggtgc ctattccgtc gtcggtgacc gcgactacga
     9061 cgtcggccag gtgctccggt tcgccctctg gcacctcgag gagctgtcgc tgtacctcgg
     9121 tatcgcgccg ttcgcggcgc tgatcatcct cgtcggcctg ggccgctcgc ggacgcgtcc
     9181 gacgaaggcc tttctggccg ccacgctcgc cctgaccgcg tggttcctgg tggtcgtggc
     9241 ggcattcgcg tccgagttcg cgaatcggat ccaggagcgc aacctgttcg ttgtaggggc
     9301 cctcttcctc gtcgccctcg tcgcctgggt cgagcagcgg gccccacggc ccctcgccct
     9361 cacgatcggg gcgctcgctc tcgccggcgg gctgccgctg ctgttcccgt acgggcgctt
     9421 catcgagacc gcggcggtct ccgacactct ggcgctcctg ccgatctgga cggccttcgg
     9481 aagcctgttg ctggactcga tcgtcttcac ggtggccgtg ggagtcgttc tcatgtgcct
     9541 gctcttcgcc ttcgtgcccg cgcgctacgc ggtgctcgtg ccgctcgccg tcctcgtctg
     9601 gttcgcgatc atgttcaagc cggtgtggag cgggccccac ggcttcaaac aggccggcat
     9661 cggagccctc ttccagggca tccgtggggt cgagcggggc tggatcgacc gcgccgttcc
     9721 ggccggtgag gaggtcgccg tcgtctggac cgggcgctcg gatcgcttca ccgtgaacga
     9781 gaacgagttc ttcaaccgtc gggtcggccc cgtctactac gttggtggac cgacccccgg
     9841 aggcctcgcc gagaccgagg tgacgatcgg cgaggacgac ggtgtcatcc gcctgctgga
     9901 cggctcgccg gtgaaggcca cctacgtgct cgtcgacgac tcgatcgcgc ccgacggcga
     9961 gccgatcgct cgtgacgagc tcctgggaat gacgctctgg cgggtcaagg gcgatctcgt
    10021 ctcgaccacg accgtggagg gcctctatcc caacgacacg tggtcggggc ctcaggtcgt
    10081 ctacacgagg cagcgctgtg agggtggcac cctctccgtg tcgctctcga gcgaccccac
    10141 gcttttcgtc ggcaaacgca cgaccgtcgt cggccggtcg gaagaaggag tcgaggagtt
    10201 cgtcaacttc ggcgcctcgg agacgacgga gctgagcgtc ccgctcgtct cgcgagacga
    10261 ccgctgtgtc gtcgtcttcg acatctcgcc gacgctcgtg ccggccgagg tcctgcccgg
    10321 taacagcgac gaacgcgacc tcggcgcgca tttcaactcc ttcgtctacg agccgcctcc
    10381 aagtagatga gaatcgcgtt cgacgtctct ccgctctccc acccacggac tggcatcggg
    10441 cagtacctca gagggtcgct cgccggtctc gcccaagcgg cggccgccga gggtggtcac
    10501 gagatcatcg ccttcgcgcc caccagcccg cgtggccggc gcgcgatccc cgaggctctg
    10561 gcgggcatcc ccgttcagct gcgcctgcgc ctgcttccct tctcacatgc attccgcatg
    10621 gcctggagcc ggcgcggccg tccaccggtt gagcgctttc tggggagggt ggacgccctc
    10681 catttcagcg actggatgta tccgccgcag gagggcgggg tgcgtgcgac cacgatccac
    10741 gatctcgttc cgctgcgctt cccggagtgg acgaccgagc gcacccgcga gatgcacggc
    10801 gagaagtacg agaacgcagc agcgacgtgc gacgtgatct tcgtcaactc cgcctacacg
    10861 gcggccgatg tgcgcagtcg gctcggcgtg cccgagaacc gcatccggat cgcgcggccg
    10921 ggtctgagcg agcgcttcaa cccgggcgga gaggtggctg atctcggccg accgtacctg
    10981 ctcgggctcg gtaacctcga gccgcgcaag aacctggttc agctcgtagc cgcccgccgc
    11041 ctccttcccg atgcgccggc gctcgcgctc accagcgggg gggagtgggg ggacggcgtc
    11101 cgtctcgatg accccgcgat cctgaaactc ggcttcgtcg acgacgaaga ggttccgcga
    11161 ctccttcgcg gcgccgacgt gttcgtgtat ccgtcgctct tcgagggctt cggcatgccg
    11221 atcgtggagg cgatggcgtg cggcacgccg gtcgtcgcct cctcgcatcc ctcgatggac
    11281 gaggcctgcg gcgacgcggc gatccgggtc gatccggagg atgcagaggc gattgccgag
    11341 ggcattcagg aagcccgtga gcggcgggtc gagctcgtcg agcgtgggct cgagcacgcg
    11401 gcgggcttca cctggcgtcg cgtcggcgac gtgtttctgc gcggctatcg ggaggcggcg
    11461 gcgtgagagt cggcctcgac gtagccccgc tcgtccagac gcaggctggg acggctcgtt
    11521 gggtgcgcgg cctgctcggc gcgctcgagc ggcaccgaga cgtcgagccg gtgccgatca
    11581 cgtggggcgg cgaggggcgc gtcacggcgg ccgtgcgcga cgtcgtctgg tatccgctgc
    11641 tgctgccacg gcgggctcgc accggccgct tcgacctcgt tcacaccacg atctaccgcg
    11701 gtcccctccg gtcgggtgtc ccggtcgtcg tgacggtgca cgatctcgct gtgctccggt
    11761 accccgacgc gttcccggcg tggacgaggc tctatggccg caccgccctg cggcggactc
    11821 tccgcgccgc gcgccgcgtc gtcgccgtct cggagttctc gcggcgcgag accgccgcgc
    11881 tcgccggtgt gccggccgat cgcatcgacg tcgttccgaa cggaatcgag ccggtcttcg
    11941 acgcggcggg ccccgccgca gagggcgact acgtcctggc cgtcggcacc atcgaaccga
    12001 gaaagaatct cggccgggcg atcgaagcgg cgcggctggc gggtgccagc cttcgcgtgg
    12061 tcggagcccg cgggtggggg aacgttcaag ccgagggtcc aggcgtcgag tggctgggcc
    12121 gcgtgtccga cgaggagctc gcgtgtctgt atcgcggcgc gcgcagcctc gtctatccat
    12181 cgctgtacga gggcttcggg atcccggtgg ccgaggccat ggcatgcggc tgtccggtgg
    12241 tgacgagtgc cggcagcgcg atggccgacg tcgccggcgg cgccgcgatc ctggtcgatc
    12301 cacacgacgt cggagcgatc gccgccggga tcgacgaggc cgatcggcgg cgcgacgtac
    12361 tcgttccgct cggtctcgag cgcgcccggg cgttcacctg ggatcgtgcg ggccaggcgg
    12421 cggtcgccgc gtaccggaga gcgctcgcgt gagccggccg ctcgtcgtgg tcgacgccga
    12481 cgttctcggc cgccgccgca ccggcgacga gacgtacatg cgaaacctcc tgcgggagct
    12541 gccggctccc gcaggggagg cgggcctgcg cctggcggcg gtgacgaggc ggcccgagct
    12601 cgttccccaa gggatcgagc cgctgccgct gtcgatcgcc ccgcaggagc tgcggatggc
    12661 gtggtcgctg cctcggctcc tccgacgagt tgacgcggcg ctcgtccaca cccagtacgc
    12721 gtgcccacct cgctctccgt gcccggcggt cgtgacggtt cacgacctct cgttcgagcg
    12781 cgatccttct ctcatgagcg cgaaagacag gctggtcttc cgtgtgatcg tgccgcgctc
    12841 cgtccggcgg gccgcgagag tcctcaccgt ctccgatcgg accaagctcg atctcgagga
    12901 cctgtacggc gtccctcccg agcacatcgt cgtgacgccc aacggcgtcg accccgtctt
    12961 cacccccgag ccgcctccgt tcgtcgacgg caacggctcg gcgccggatg cctacatcct
    13021 ctcggtcggc gcgatccagc gacggaagaa ccaggaggcc gctctacgtg ccgcccggca
    13081 agccggcatt gagctcggcg tcgtaggtcc ggagaagg
//
LOCUS       DBNGNK010000159        10325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1603705,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000159 DBNGNK010000000
VERSION     DBNGNK010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10325
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1603705"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="AC150V_04590"
     CDS             complement(<1..1050)
                     /locus_tag="AC150V_04590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762233.1"
                     /translation="MSEQKQRSPLRQRSIAALLTAEAISATGSQMTFLALPWFVLATT
                     GSAAKTGIVLAVELAPIALLGIPSGTVVSRLGARRTMLVGDLGRAPLMAAIPILHSAG
                     LLSFGVLLAIVAAFGVFLAPYFSAQRLVIPELVGDDEQTVAQGNALVEGVQRVTALIG
                     PAVAGVLIATMGATAVLYIDAATFVVSFAILALLVPQRPPAAPTEESKGVLAGVRYLM
                     RDRVLGPLGVTALFLNMFGQMLVIAIPVLAFEEFGESSRLAGMFFAAWGAGAVVGAVA
                     AMKLVTRFEPIRLGAAALVALTVPLWLLALPLPAAAVAAVLFASAFFGPLVNAPLIAV
                     ITTRTPKALRAKVMTA"
     gene            1182..1757
                     /locus_tag="AC150V_04595"
     CDS             1182..1757
                     /locus_tag="AC150V_04595"
                     /inference="COORDINATES: protein motif:HMM:NF025343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3762234.1"
                     /translation="MPKISEEAKQARREQILAGARRCFARYGYEGATVARLEEEIGLS
                     RGAIFNWFPSKEELFIELATRDNIRLLQLIVDRGLEETVSALAEEDADWLAVYFEFGR
                     RLRASEELRSRWKELVPAELRERADAWVVEGQRSGELRDDMKPEEIGVFLGALFDGIV
                     VRRALGFENPDPEPLLKLMRAAIGGPATPDR"
     gene            complement(1783..2559)
                     /locus_tag="AC150V_04600"
     CDS             complement(1783..2559)
                     /locus_tag="AC150V_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR3762235.1"
                     /translation="MSDFRHTARKAALLRSLHSAERPLVLPNAWDVASARLVEDAGFA
                     AVATGSAAVAASLGFEDHEHAPADAMLAAAARIARSVAVPVTVDAEAGYGLTPGDLAA
                     GLIEAGAAGCNLEDSDHDGGGLVAAEVQAGRIAALRDAARAAGVELVVNARVDVFITE
                     WGEPGERLDEALRRSRLYVESGADCVYPIWAAEEHVIGALAARLDAPVNVLYRPGAPS
                     FAVLEQLGVRRITFGPGLHRVAEAATQSALKKIAAHEEPY"
     gene            complement(2556..3116)
                     /locus_tag="AC150V_04605"
     CDS             complement(2556..3116)
                     /locus_tag="AC150V_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR3762236.1"
                     /translation="MTRTLAFLLAGVLLAGVAGFAVVQAEPDWYQRFRYPLRYEHIVT
                     GHAENYDLDPALLAAVIYSESKFEADARSAAGAVGLMQLLPETAKGIALRTGGKKFVV
                     GDLVNPEINVRYGSWYLRNLLDKYGDERLALAAYHAGQGNVDAWRKSGVGIQFPETRA
                     YVDKVQRLKQVYAGAYAGELAPSGVR"
     gene            complement(3113..3706)
                     /gene="coaE"
                     /locus_tag="AC150V_04610"
     CDS             complement(3113..3706)
                     /gene="coaE"
                     /locus_tag="AC150V_04610"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF013301.7"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR3762237.1"
                     /translation="MRPVAVAITGGIGAGKTAALDSFRRHGAATVSSDEIVHHLLAED
                     EDVRAALVERFGAGVLGADERPDRGKIAEIVFGDPEKLAFLESLLHPLVSREYLRWRD
                     QLSQLPTPPRVCVTEVPLLYEVGSEERFDKVVVITAPRALREQRRRVPLDRRDDRLLP
                     DREKVRRADFHYRNTGSLEELDNWVAGVMAELSKEPE"
     gene            complement(3714..5234)
                     /gene="rpsA"
                     /locus_tag="AC150V_04615"
     CDS             complement(3714..5234)
                     /gene="rpsA"
                     /locus_tag="AC150V_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR3762238.1"
                     /translation="MANDTVEKPTDTEEGVWTTGPDGELVPDYDATFPTINEGEVVHG
                     TVVRVDKDEVLVDIGYKSEGVIPVAELSIRRSVNPEDEVAVGEEIDALVMTKEDAEGR
                     LILSKKRARFELAWKAIERAAESGEPVNGRVIEVVKGGLILDLGVRGFLPASLVDIRR
                     VQDLDEFLSQELRCKVIELNRSRNNVVLSRRAVLEDERKEQRQQILDRLNAGDIVTGH
                     ISNIVAFGAFVDLDGMDGLIHISELSWSHVNHPSEVLEIGEEVEVKVLDIDRERQRIS
                     LGLKQTQSDPWQQVLDSYSQGDVVEGRVTKVVTFGAFVEILPGVEGLVHISELAQHHV
                     ENPREIVSQGDPVNVLILEIDGDRRRLSLSLKRVEEGVQPVPRADGAESVHATPDLKL
                     SEEAFPSGEQAPAEAAPVEDAAPVEDAARVEDAAPVEDAAPVGETAPVEMLEASAAPA
                     VEAESDPIEEASIEVEVAPPEAEAAAEPVAEQVAEAPPEPEPAEATEPESADDDSA"
     gene            complement(5299..7872)
                     /locus_tag="AC150V_04620"
     CDS             complement(5299..7872)
                     /locus_tag="AC150V_04620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012688.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR3762239.1"
                     /translation="MSPKTLTGAELQLDDGPARDAELFLVDGNNLAYRAFYALPEELA
                     TSEGFSTNALLGFTNMLFKLLSDYRPRGVAVAWDTRPTAREEISETYKEGRKPMPGLL
                     REQFPHLRPIVDAFGYENLEFEGWEADDVIATIATLADSRALKTCIVSTDRDAFQLCT
                     ENVCLMMTPRGVSDVHVYTPDRVEARYGVRPDQVPDFIGLKGDTSDNIPGVPGIGDKT
                     AGQLISQYGSLESVLEHVDELSPARSKSIREHADQARESKELATMRRDLPLDVDPTDL
                     VAGAPDRSELKEIFRRFEFRALLGRIDELDTALPAAERPRVTGQSVPWREGELAAVTG
                     RVGLAVEHARFALAAEDGVVVGEWSGPPAELRDAEIVAHDGKRLRLPFPPADDTLIAA
                     YLIEPGRSEYLLDDLLAEYGLELEPEPAAEEETAALIRHAEGPRRLAGPMRKRLAERG
                     SERLYDDVELPLSAVLAAMEDAGVLIDTYRMGEITARLADRVEELEARAHELAGEEFV
                     LGSPQQLSRILFEVLELTPGRKGKTGYSTDSRVLRSIRDEHEIVGVIEEWREYAKLLN
                     TYLEPLPTLISADDGRLHTTFNQTVAATGRLSTSNPNLQSIPIRTDLGRQIRSAFVAA
                     EGSRLLSADYSQVELRILAHVSGEPALREAFERGDDIHAATAAEVLGKEQATLTKDER
                     NVAKMVNFGIIYGISAFGLSENLEIPREEAQEYIDTYLARFPRVQEFIERTIAEATED
                     GYVTTLMGRRRPIPELRTQNRQTRSLGERLAVNTVMQGTAADVIKIAMVRLQERLHAE
                     GRGARLVLQVHDELLLEVPETELTALRTLVREEMVGAYPLDPPLAVDVGVGDDWAEAK
                     A"
     gene            7898..8833
                     /locus_tag="AC150V_04625"
     CDS             7898..8833
                     /locus_tag="AC150V_04625"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3762240.1"
                     /translation="MKALEIRSLDDGLLDDAGALLADRHSRHRAAEALLPAVEDFRAQ
                     IERDLSRERAVGVAALRYGRLVAYLVGRSESRGPFEWGFVDLAGCAASEAEAVRDLFS
                     ALASDWHEHGISRFGAMVPASDRDLIDVWFRLAFGCQFVTAVRETEKEQPIDADVEIR
                     VGGPSDLEAVASFDRLLWTLQADSPSFSGADVDAENFRREWNDTWDDPDEFPHFVAER
                     EGRAVGHVLLYRRPGGDLRVPEQNIDLAHAATLDDVRGSGVGRALTAHALRWSYEQGY
                     RSMTTDWRSVNLLSSRFWTRRGFRSTFLRLYRAVP"
     gene            8848..>10325
                     /locus_tag="AC150V_04630"
     CDS             8848..>10325
                     /locus_tag="AC150V_04630"
                     /inference="COORDINATES: protein motif:HMM:NF021387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR3762241.1"
                     /translation="MPRLPMLAGSRVSVLNAPDDAVVLRPPPPLDPLIDVAAAVDEAL
                     RYPLSGPSIDEVATPRTKATIVVDPPILPLPGVPSDPRQDALSGTISTLERAGVPSEQ
                     QTILVARGLERRAGSKELEGLLHPAQARRFHGEVVVHDCESPDLRQLGQADGIPLRVN
                     QALTDADLVIVVSAAETVLHGGPAVLLGAGGPELARAAVVAESLLATHGAPGWDVAVS
                     LERLLDARAPLVGVSLLLDHPRYTGRLRDYPHNPEADRRAAPRLARSIHGMLPGSARR
                     RVLRDLGRELPAVSAFAGPPRVAHIEALLRGISLRATHVREQLDALVVPLPWRSAHLP
                     PEPIDPITASAVALGLALRLWRERFPIVDGGTAVLLQSFSRSFGHGAGEPYSAFFDSI
                     RRSRDPSDLADAEAQASRDTGAIDAYRSGRAPHPLLPYADWASCAPALGRLGRVIVAG
                     CRDAGAARALGFIPSHSHSAALEMAYGVAAGRARVGFLLAPP"
BASE COUNT         1518 a         3674 c         3524 g         1609 t
ORIGIN      
        1 ggccgtcatc accttggccc gcagtgcctt cggcgttcgt gtggtgatca cggcgatcag
       61 tggcgcgttc acgagcgggc cgaagaacgc agacgcgaac agcaccgcgg ccacggccgc
      121 agcgggaagg ggcagggcga gcagccagag cggcaccgta agcgcgacca gcgcagcggc
      181 gccgagccga atcggctcga agcgggtgac gagcttcatc gcagcgaccg caccgacgac
      241 cgcccccgcc ccccaggcag cgaagaacat gcccgccagc cgcgagctct cgccgaactc
      301 ctcgaaggcg agcacgggga tggcgatgac gagcatctgc ccgaacatgt tcaggaacag
      361 cgccgtcacg ccgagcgggc cgagcacccg gtctcgcatc aggtaccgga caccggcgag
      421 cacccctttg ctctcctcag tcggagcagc cggcggccgt tgcggcacca gaagggcgag
      481 aatcgcgaac gagacgacaa aggtcgccgc gtcgatgtac agaaccgccg tcgcacccat
      541 cgtcgcgatg agcactccgg cgaccgccgg cccgatcaga gcggtgacgc gctggacgcc
      601 ctcgacgagc gcgttgccct gcgccaccgt ctgctcgtcg tcgcccacca gttccgggat
      661 gacgagccgc tgtgccgaga agtagggggc gagaaagacc ccgaaggcgg cgacgatcgc
      721 gagcaggacg ccgaacgaga gcagacccgc gctgtgcaag atcgggatcg ccgccatcag
      781 aggcgcccgc ccgaggtcgc cgacgagcat cgtccgccgc gccccgaggc gcgacacgac
      841 cgtgccgctg ggaatgccga gaagcgcgat gggcgcgagc tcgaccgcga ggacgatgcc
      901 cgtcttcgcc gccgaccccg tcgtggcgag gacgaaccag ggaagagcca gaaacgtcat
      961 ctgcgagccg gtcgccgaga tcgcctcggc cgtgagaagg gcggcgatcg aacgctggcg
     1021 aagcggcgag cgttgcttct gctcagacat gccaggtgcc gtgctgctga gcgttctcgc
     1081 cgtcgagctc gatcccgacg ccgtcgatgc gcgggtactg gagggcgatc gtcacggcgc
     1141 tgcgataata aaacgaacgt actgttttgt aaagtgaggg cgtgccgaag atctcagaag
     1201 aggcgaaaca ggcccgccgc gagcagattc tcgctggcgc ccggcggtgc ttcgcccgtt
     1261 acggctacga gggcgccacc gtcgcgcggc tcgaagagga gatcggcctc tcgcgcggcg
     1321 cgatcttcaa ctggttcccc tcgaaggagg agctgttcat cgagctcgcc acccgggaca
     1381 acatccgcct gctgcaactg atcgtcgatc gtggcctgga agagaccgtc tccgccctgg
     1441 ccgaggagga cgccgactgg ctggccgtct acttcgagtt cggcaggcgc ctgcgcgcgt
     1501 cggaggagct ccgttcccgg tggaaggagc tcgttccggc agagctgcgg gagcgggccg
     1561 acgcctgggt cgtcgaggga cagcggagcg gcgagctccg ggacgacatg aagcccgagg
     1621 agatcggtgt cttcctcgga gcgctcttcg acggcatcgt cgtccgtcgc gctctgggct
     1681 tcgagaatcc agaccccgag ccgctcctca agctcatgcg cgcggcgatc ggcgggccgg
     1741 cgacgcccga ccgctgaggc ccgagaagca tcgccgcgcc ggttagtacg gctcttcgtg
     1801 ggcggcgatc ttcttcagcg cagactgcgt cgcggcctcc gcgacgcggt gcagtccggg
     1861 cccgaaggtg atgcggcgca ccccgagctg ctcgagaacg gcgaaggacg gggcgccggg
     1921 ccggtagaga acgttgaccg gtgcgtcgag gcgagcagcg agtgcgccga tcacgtgctc
     1981 ctccgccgcc cagatcgggt acacgcagtc ggcgccggac tcgacgtaga gccggctgcg
     2041 acgcagggcc tcgtcgagcc gctcgcccgg ctcgccccat tcggtgatga agacgtcgac
     2101 acgggcgttg acgaccagct cgacgcccgc agcgcgcgcg gcgtccctta gagccgcgat
     2161 ccgcccggcc tgcacttcgg cggcgacgag cccgccgccg tcgtggtcgg agtcctcgag
     2221 gttgcagccg gcggctcccg cctcgatcag cccggcggcg aggtcgccgg gtgtcagccc
     2281 gtagccggcc tcggcgtcga cggtgacggg gaccgccact gatcgggcga tccgcgcagc
     2341 ggccgctagc atcgcgtccg cgggagcgtg ctcgtgatcc tcgaaaccga gcgacgcggc
     2401 gacggcagcg ctcccggtcg cgaccgctgc gaagccggcg tcctcgacca gcctggcact
     2461 tgcgacgtcc catgcgttcg ggaggacaag cggccgctcg gcgctgtgga gcgagcggag
     2521 cagcgccgcc ttacgggccg tgtggcggaa gtcgctcatc tcactccact cggtgcgagc
     2581 tcgccggcgt aggcgccggc gtagacctgc ttcagccgct ggaccttgtc gacgtaggcc
     2641 ctcgtctccg ggaactggat cccgacaccg ctcttccgcc aggcgtcgac gtttccctgg
     2701 ccggcgtggt aggccgccag cgccagccgc tcgtccccgt acttgtcgag caggttgcgc
     2761 aggtaccagg acccgtaccg gacgttgatc tcggggttga cgagatcgcc gacgacgaac
     2821 ttcttcccgc cggttcgcag cgcgatcccc ttggccgtct cgggcaggag ctgcatcagc
     2881 ccgaccgcgc cggccgcaga ccgcgcatcg gcctcgaact tgctctccga gtagatgacg
     2941 gcggccaaca gcgccgggtc gagatcgtag ttctcggcat ggccggtgac gatgtgctcg
     3001 tagcgcagcg ggtagcgaaa ccgttggtac cagtccggtt ccgcctgcac gaccgcgaac
     3061 ccggccacgc cggcgagcaa caccccggcg aggaggaacg caagcgtccg tgtcactccg
     3121 gctccttcga caactcggcc atcacgcctg cgacccagtt gtcgagctcc tccaggcttc
     3181 cggtgttgcg gtagtggaag tcggcccgcc gcaccttctc acggtcgggt agcaggcgat
     3241 cgtcgcgacg gtcgagcggc acgcgccggc gctgctcacg cagggcgcgc ggcgccgtga
     3301 tcacgacgac cttgtcgaag cgctcctccg acccgacttc gtacagaagt ggcacctcgg
     3361 tcacgcagac gcgcggcggc gtcggaagtt gggaaagctg atcacgccac cgaagatact
     3421 cgcgcgagac gagtggatgc agcaggctct ccaggaacgc gagcttctcg ggatcaccga
     3481 agacgatctc tgcgatcttg cctcggtcgg gccgctcatc cgctcccagg acgcccgcgc
     3541 cgaaccgctc taccagagcg gcccgcacgt cctcgtcttc ggccagcaga tgatggacga
     3601 tctcgtcgct ggaaacggtg gccgcaccgt ggcggcggaa agagtccagc gcagcggtct
     3661 tgccggcgcc gatgccgcct gtgatggcga ccgcgaccgg ccgcacgacg gagtcaggcg
     3721 ctgtcgtcgt ccgccgactc gggctcggtc gcctcggccg gctcgggctc cggcggcgct
     3781 tccgcaacct gctctgccac tggctcggct gcggcctcag cctccggcgg cgcgacctcg
     3841 acctcgatcg aggcctcctc gatcggatcg ctctctgcct cgactgccgg cgccgcggaa
     3901 gcctcgagca tctcgaccgg ggccgtctct ccgaccgggg ccgcgtcctc gaccggggcc
     3961 gcgtcctcga cccgggctgc gtcctcgacg ggggctgcgt cctcgaccgg cgccgcctcc
     4021 gcgggcgcct gctcgccgct cgggaaggcc tcctcagaga gcttgagatc cggcgtcgcg
     4081 tgcaccgact cggcgccgtc cgcgcgcgga acgggctgga cgccctcctc gacacgcttg
     4141 agcgagagcg acagccgcct gcggtcgccg tcgatctcga ggatcaagac gttcacgggg
     4201 tcgccctgag agacgatctc cctcgggttt tcgacgtggt gctgtgcgag ctcggagatg
     4261 tggacgagcc cctcgacacc cgggaggatc tcgacgaagg caccgaaggt gacgaccttc
     4321 gtgacgcggc cctcgacgac gtctccctgc gagtaggagt cgagcacctg ctgccacgga
     4381 tcggactggg tctgcttgag accgagggag atccgctggc gctcgcggtc gatgtcgagc
     4441 accttcacct cgacctcctc gccgatctcg agcacctccg acggatggtt cacgtggctc
     4501 cacgacagct cggagatgtg gatcagcccg tccataccgt cgagatcgac gaaggcgccg
     4561 aaggcgacga tgttggagat gtgccccgtg acgatgtcgc cggcgttgag ccggtcgagg
     4621 atctgctgcc gctgctcctt gcgctcgtcc tcgagcacgg cgcggcgcga gagaacgacg
     4681 ttgttgcgcg agcggttgag ctcgatcacc ttgcagcgga gctcctggct gaggaactcg
     4741 tccagatcct ggacgcgcct gatgtctacc agcgaggcgg gcaggaagcc gcgtacaccg
     4801 agatcgagga tcagtccacc cttcacgacc tcgatgacgc gtccgttgac cggctcgccg
     4861 ctctcggcgg cccgctcgat cgccttccag gcgagctcga agcgggcgcg cttcttggag
     4921 aggatcagcc ggccctcggc gtcctccttc gtcatcacga gcgcgtcgat ctcctcgccg
     4981 acggcgacct cgtcctcggg gttcacggag cgccggatcg agagctcggc gacggggatc
     5041 acgccttcgg acttgtagcc gatgtccacg agcacctcgt ccttgtccac ccggacgacc
     5101 gtgccgtgca cgacctcgcc ttcgttgatc gtcgggaagg tcgcgtcgta gtcgggtacg
     5161 agctccccgt cggggcctgt cgtccagacc ccttcttcgg tatccgttgg cttttccacc
     5221 gtatcgttcg ccatagcgag ccgtggagta tagcccggtt ggggaaaaca ggcccatccg
     5281 gcgagggccc gtacgtcgtc aggccttggc ctcggcccag tcgtccccga ctcccacgtc
     5341 gaccgccagc ggcgggtcga gtgggtaggc accgaccatc tcctcgcgca ccagcgtgcg
     5401 cagcgcggtc agctccgtct cggggacttc gaggagcagc tcgtcgtgca cctgcaggac
     5461 gagacgggcg cctcgcccct ctgcatgcag ccgctcctgg aggcgaacca tcgcgatctt
     5521 gatcacgtcg gcggcggttc cctgcatcac cgtgttgacc gccagccgct cgccgagtga
     5581 gcgtgtctgc cggttttgcg tccggagctc gggaatcggt cgccgccgcc ccatgagggt
     5641 cgtcacgtag ccgtcctccg tcgcctcggc gatcgtccgc tcgatgaact cctgcacgcg
     5701 cgggaaacgg gcgaggtacg tgtcgatgta ctcctgcgcc tcctcgcggg gaatctcgag
     5761 gttttccgac aacccgaagg cggagatccc gtagatgatc ccgaagttca ccatctttgc
     5821 gacgttgcgc tcgtccttcg tgagcgtcgc ctgctccttc ccgagcacct ccgcggcggt
     5881 ggcggcgtgg atgtcgtcgc ctcgctcgaa ggcctcacgc agcgccggct cgcccgagac
     5941 gtgggcgagg atgcgcagct cgacctggct gtagtcggcc gacagcagcc ggctcccttc
     6001 cgccgcgacg aacgcggagc ggatctggcg gccgaggtcg gtgcgaatcg ggatcgactg
     6061 gaggttgggg ttcgacgtcg agagccgccc cgtcgcggcg acggtctggt tgaacgtggt
     6121 gtgcaggcgc ccgtcgtccg ctgagatcag ggtcggcagc ggctcgaggt acgtgttcag
     6181 gagcttcgcg tactcgcgcc attcctcgat cactcccacg atctcgtgct cgtcgcggat
     6241 cgagcggagc acgcggctgt cggtcgagta gccggtcttc cctttccggc cgggcgtgag
     6301 ctccagcacc tcgaagagga tccgcgagag ctgctgcggc gagccgagca cgaactcctc
     6361 gcctgcaagc tcgtgcgcgc gcgcctcgag ctcctcgacc cgatcggcga ggcgtgcggt
     6421 gatctcgccc atccgatacg tgtcgatgag aacgccggcg tcctccatcg cggcgagcac
     6481 ggcggagagc ggcagctcga cgtcgtcgta gagccgctca gagcctctct cggcgagccg
     6541 cttccgcatc ggtcctgcca gcctgcgtgg gccctcggcg tgtcggatga gggccgccgt
     6601 ctcctcttcc gccgccggct cgggctcgag ctcgagcccg tactcggcga gaaggtcgtc
     6661 gagcaggtac tcggaacgcc ccggctcgat caggtaggcg gcgatcagcg tgtcatcggc
     6721 aggcggaaac gggagtcgga ggcgcttgcc gtcgtgcgcg acgatttccg cgtcacgcag
     6781 ctcggccggc ggccccgacc actcccccac cacgacaccg tcctcggctg cgagcgcgaa
     6841 gcgggcgtgc tcgaccgcca gtcccacgcg gccggtgaca gcagccagct ccccctctcg
     6901 ccaggggaca gattgacccg tgacccgtgg ccgctctgcc gccgggaggg cggtgtcgag
     6961 ctcgtcgatc cggccgagga gcgcacgaaa ctcgaagcgg cggaagatct ccttcagttc
     7021 cgaccggtcg ggcgcccccg cgacgagatc cgtcggatcc acgtcgagcg ggaggtcgcg
     7081 acgcatcgtg gcgagctcct tcgactcacg cgcctggtcg gcatgctcgc gaatcgactt
     7141 cgagcgcgcg ggcgagagct cgtcgacgtg ctcgagcacg gactccagcg agccgtactg
     7201 cgagatcagc tggcccgccg tcttgtcgcc gatgcccggg acgcccggaa tgttgtcgga
     7261 cgtgtccccc ttgaggccga tgaagtcggg cacctggtcg ggacggacgc cgtatcgagc
     7321 ctcgacacga tcgggcgtgt acacgtgcac gtccgagact ccgcgcgggg tcatcatcag
     7381 gcatacgttc tcggtgcaca gctggaaagc gtcccggtcg gtcgagacga tgcaggtctt
     7441 gagcgcccgg gaatcggcga gagttgcgat cgtggcgatc acgtcgtccg cctcccaccc
     7501 ctcgaactcg aggttctcgt acccgaaggc gtccacgatc ggccgcaagt gcgggaactg
     7561 ctctcgcaga aggcccggca tcggctttcg gccctccttg taggtctccg agatctcctc
     7621 gcgcgcggtc ggccgtgtgt cccacgccac tgcgacccca cggggccggt aatccgacag
     7681 cagcttgaag agcatgttcg tgaagccgag cagcgcattg gtcgagaacc cctcgctggt
     7741 cgcgagctcc tctggcagcg cgtagaaggc gcggtaagcg aggttgttcc cgtcgacgag
     7801 gaacagctcc gcgtcgcgtg cggggccgtc gtcgagctgc aactccgccc cggtcagtgt
     7861 cttcggcgac acgcagcaac ggtatcgtcc ggcgccgatg aaggcgctcg agatcaggtc
     7921 actcgacgac ggcttgctcg acgacgcagg cgccctgctc gccgaccggc actcacgtca
     7981 ccgcgccgcg gaggcgttgc tgccggcggt cgaggacttc cgcgcacaga tcgaacggga
     8041 tctgtcgcgc gaacgcgcgg tcggggtggc cgcgctccgc tacggccgcc tcgtcgcgta
     8101 cctcgtcggc cgcagcgagt cacgtggccc gttcgagtgg ggcttcgtcg acctcgcagg
     8161 ctgcgcagca tccgaggccg aggcggtgcg cgatctcttc tcggccctcg ccagcgactg
     8221 gcacgagcac gggatcagcc gcttcggcgc gatggttcca gcctccgacc gagacctgat
     8281 cgacgtctgg ttcaggctcg cgttcggctg ccagttcgtc acggccgtga gagagacgga
     8341 gaaggagcag ccgatcgacg cggacgtcga gatccgcgtg ggcggcccgt cggatctcga
     8401 ggcggtcgcg tcgttcgacc ggctcctctg gacactacag gccgattcac cgagcttctc
     8461 cggtgccgac gtggacgccg agaacttccg ccgcgagtgg aacgacacgt gggacgaccc
     8521 cgacgagttc ccgcatttcg tcgcggagcg cgaggggcgc gcggtcggcc atgtgctgct
     8581 ctaccggcgg ccgggcggcg acctgcgcgt ccccgagcag aacatcgacc tcgcccacgc
     8641 tgcgacgctc gacgacgttc gcggcagcgg cgtcggacgc gcgctgaccg cgcacgcact
     8701 acgctggtcg tacgagcagg gctaccggtc gatgacgacc gactggcgga gcgtcaacct
     8761 cctctcatcc cgattctgga cgcgccgcgg attccgctcg accttcctgc gcctctatcg
     8821 cgcggtgccg tgaggagacg ctgaggcatg ccgcgccttc cgatgctggc cggctctcgc
     8881 gtgtccgtcc tgaacgctcc cgacgacgcg gtcgtgctgc gcccgcctcc gccgctcgat
     8941 ccgctcatcg acgtcgcagc agcggtcgac gaggcgctcc gctatccgct ctcggggcct
     9001 tcgatcgacg aggtggcgac cccgcggacg aaggcgacga tcgtcgtcga cccgccgatc
     9061 ctgccgctgc ccggcgtccc gagcgacccc cgccaggacg cgctttcggg cacgatctcg
     9121 acgctcgagc gggccggcgt gccatccgag cagcagacga tcctcgtcgc ccgggggctc
     9181 gagcgccgcg ccggctccaa agagctcgag gggctgcttc accctgcgca ggcgcggcga
     9241 ttccacggcg aggtcgtcgt tcacgactgc gagagccccg acctccgcca gctgggtcag
     9301 gcagacggca tcccgctccg cgtgaatcag gcactgaccg acgcagatct cgtgatcgtg
     9361 gtctctgcag ccgagaccgt gcttcacgga gggcccgccg tgcttctcgg cgccggcggg
     9421 ccggagctgg cgcgggccgc cgtcgttgcc gagtcgctcc tcgccacgca cggcgctcct
     9481 ggctgggacg tcgccgtttc gctcgagcgg ctgctcgacg cccgggcgcc gctcgtcggc
     9541 gtctcgctcc tgctcgacca cccgcgctac acgggacgcc tccgcgatta tccgcacaac
     9601 ccggaggccg accggcgcgc ggccccgagg ctggcgcgga gcattcacgg catgcttccc
     9661 gggagcgctc ggcgccgcgt gctccgcgac ctcgggcgtg agctgccggc cgtctcggct
     9721 ttcgcggggc caccacgcgt ggcccacatc gaagcgctgc tgcgcggcat ctcgctgcgt
     9781 gcgacgcatg tccgagagca gctcgacgca ctcgtcgtcc cgctgccgtg gaggtctgcc
     9841 cacctcccac ccgagccgat cgatcccatc acggcctcgg cggttgccct cggcctcgca
     9901 ctcaggctct ggcgggagcg cttcccgatc gtcgacggag gcacggcggt gctgctccag
     9961 agcttctccc gctcgttcgg ccacggtgcc ggcgagccgt actccgcctt cttcgactcc
    10021 atccgccgca gccgcgaccc ttccgacctc gccgacgccg aagcgcaggc gtcgcgcgac
    10081 accggcgcga tcgacgccta ccgaagcggg cgggccccgc atccccttct tccgtacgcg
    10141 gactgggcct cgtgcgcgcc cgctctgggc cggctggggc gggtgatcgt ggccgggtgc
    10201 cgcgacgccg gagcggctcg cgcactcggc ttcatcccga gccacagcca ttccgccgcg
    10261 ctcgagatgg cctacggcgt cgccgcaggc cgcgcgcgtg tggggttcct gctcgcgcct
    10321 cccta
//
LOCUS       DBNGNK010000160         4723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1388169,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000160 DBNGNK010000000
VERSION     DBNGNK010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1388169"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="AC150V_04635"
     CDS             <1..460
                     /locus_tag="AC150V_04635"
                     /inference="COORDINATES: protein motif:HMM:NF019325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HIR3762242.1"
                     /translation="VANTDLYTARSMAAIWQRVHGASTGGTPTGLHGNSIESVTVQPS
                     GQQLSPTTETTIQASTDLAFDVAVTNSGDFQEVQVEVTLTIPKQPSPIVKKGAIDLID
                     PGETKTISFKNFPDPPFGEKTTVKVDVKPVPGEANTANNTAEYPVIFSLG"
     gene            547..1017
                     /locus_tag="AC150V_04640"
     CDS             547..1017
                     /locus_tag="AC150V_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HIR3762243.1"
                     /translation="MDTTLAVAVGACALAAVALLAAVTAWWWLARLRRSQSVLLGGGT
                     GDLVDFAVSLQGRIDDLRGVADELAAGLSRVDRRLDGAVTNTAVIRYDAYEGTGGQQS
                     ASFALLDSNRTGTVITAIQGRDYARIYVKELDHGRASVALSPEELEAVERAMSR"
     gene            1129..1659
                     /locus_tag="AC150V_04645"
     CDS             1129..1659
                     /locus_tag="AC150V_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013015063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR3762244.1"
                     /translation="MQQVLVLNASYEPLNVCSVRRAHVLVHKGKAEVLERDDKPLRSA
                     ARAFDWPHVIRLLAYVRVPRAITRRISRRVLFARDGWRCVYCGTNGGRLTLDHVVPRS
                     RGGSSIWENVVTACAPCNLRKGDRLPEEVAMPLTRQPRPPQPVLFIRLAAHRIPDVWR
                     TYLPTLDHGEPAASVA"
     gene            complement(1672..2514)
                     /locus_tag="AC150V_04650"
     CDS             complement(1672..2514)
                     /locus_tag="AC150V_04650"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF012998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR3762245.1"
                     /translation="MMMTAELTVAYPGREGAHSAAACDRLFPAARLIPLPSFADVVAS
                     VTSGSTPYGVLPIESSLSGPVAETHDLLYDSALSIVAEAILPIRHCLVGPEAVERTEI
                     REIHSHPVALDQCRALLASMPWATATASPTTGDAAGQVAEIGDSGVVAIASHRAAALN
                     KLVVVEEDVGDHPEAYTRFVSIAPYTRLDRRDGEWRTAFSFVTNHQPGALYRALEPLA
                     RHDIDLSLLASRPIPSTPWRYRFDAVLSGHPLDPEVGATLGELRELTARLRVFGSYRA
                     DREP"
     gene            complement(2668..3654)
                     /gene="leuB"
                     /locus_tag="AC150V_04655"
     CDS             complement(2668..3654)
                     /gene="leuB"
                     /locus_tag="AC150V_04655"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR3762246.1"
                     /translation="MKVAVLPGDGIGPEVAEQAVRVLDALGIEHSEHPFGAGAILAGA
                     PPLPEETLAACREADAVLVGAVGLPELEGKPVRPEQGLLALRKELGVYANIRPARAAG
                     IDLMIVRELTGGLYFGEKGTRPDGTWFDTCEYSRPEVERIARRGFEAAQARSGRLTSV
                     DKVNVLHTSRLWRDVVTELASEYPDVRLDHALVDSFAMVIVQAPQSIDVVVTENTFGD
                     ILSDVAAAVTGGLGLAASASLGDDGPGIFEPVHGSAPQIAGQGVANPAAMLRSTALLL
                     AQGLGRPTDAEALDVAVDRALAEEPTADLGGSASTASFGDHVLRSLERSPTV"
     gene            complement(3695..>4723)
                     /gene="thrC"
                     /locus_tag="AC150V_04660"
     CDS             complement(3695..>4723)
                     /gene="thrC"
                     /locus_tag="AC150V_04660"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3762247.1"
                     /translation="VEAPEQQLIPPGLTPLVPAPRLAAEVGVGELWLKLDTANPTHSF
                     KDRVVAVAARKAQELGFETLSCSSTGNLAGAVAARAAAEGLEAAVFCPADLEPEKLAS
                     AAAYGARLYAVNGTYDHCSRLSVELSFELPWGFVNVNLRSYYAEGSKTIAFEVAEQLG
                     WETPDAVVGPIASGALFHKVGQGFKEFQALGLVDGDTPRLIGGQAEGCEPVALAFREG
                     RNVQPVRPDTLARSLAIGSPADGDFAVTTARETGGGIYTVAEDEIGENMASLAGTTGV
                     FGETASGVTLGALRAAVADGRVGRDSRVVLLVTGDGLKTPQAVAHTFTPIEIEADADV
                     FVDEMLAA"
BASE COUNT          752 a         1645 c         1629 g          697 t
ORIGIN      
        1 cgtcgccaac accgacctgt acacggcccg ctcgatggct gcgatctggc agcgcgtcca
       61 cggcgcgtcg accgggggca cgccgaccgg cctgcacgga aactcgatcg agtcggtgac
      121 cgtgcagccg agcggccagc agctgtcacc cactaccgag acgacgatcc aggcctccac
      181 cgatctcgcc ttcgacgtcg ccgtcaccaa ctcgggcgac ttccaggagg tgcaggtcga
      241 ggtgacgctg acgatcccga agcagccgag tccgatcgtc aagaagggtg cgatcgacct
      301 gatcgatccg ggcgagacga agacgatcag cttcaagaac ttcccggatc cgccgttcgg
      361 cgagaagacc accgtgaagg tcgacgtcaa gccggtgccg ggcgaagcga acaccgcgaa
      421 caacactgcc gagtatccgg tcatcttctc gctcggctag acgattgatc ccgagtcagc
      481 gtccagaggc ctgatgcgag cgctgctccc cttgtctgcg gcgcgccctg agcgggagcc
      541 ctagcgatgg acacgacgct cgcggtcgca gtcggcgcgt gcgcactcgc tgccgttgcg
      601 ctgctcgccg ccgtcacggc gtggtggtgg ctcgcccgac tgcgccggtc gcagagcgta
      661 ctgctcggcg ggggtacggg cgatctcgtc gacttcgccg tctcactgca aggtcggatc
      721 gacgatctgc gcggagtcgc cgacgagctc gccgcggggc tgtcgcgggt cgaccgacga
      781 ctcgacggcg cagtcacgaa caccgcggtc atccgctacg acgcctatga gggcaccggg
      841 gggcagcaat ctgcgtcgtt tgcactgctc gactccaacc ggaccggcac ggtcattacg
      901 gcgatccagg ggcgcgacta cgcgcgcatc tacgtcaagg agctcgacca tggcagggcg
      961 tcggttgcgc tttcgccgga ggagctcgaa gccgtcgaac gcgccatgtc ccgctgatcg
     1021 accgggagag acccctgttc cctcacgagc ccgtcctctt gctcgggcgc aggagcccct
     1081 tcagaactcc ccgctcaggg ctctattgcg ctggtacatt cacggcgcgt gcagcaggtt
     1141 ctcgtcctca acgccagcta cgagccgctc aacgtctgct cggtacgccg ggcccacgtg
     1201 ctcgtgcaca aaggcaaggc ggaggtgctg gagcgagacg acaagccgct tcgctccgcc
     1261 gcgcgcgctt tcgactggcc gcacgtgatc cggctgctcg cgtacgtccg cgtgccgcgg
     1321 gcgatcacgc ggcgtatctc acgacgcgtg ctcttcgcac gtgacggctg gcgctgcgtc
     1381 tactgcggca cgaacggcgg tcgactcacg ctcgatcacg tcgtcccgcg ctcgcggggt
     1441 ggatcgtcca tctgggagaa cgtcgtcacc gcctgcgcgc cctgcaatct ccgtaaaggt
     1501 gatcgcctgc cggaggaggt cgcgatgccg ctcacgcgcc agccgagacc gccacagccg
     1561 gtgttgttca tccgcctggc ggcgcaccgc atcccggacg tctggcgtac gtacctgccg
     1621 acgctcgacc acggcgagcc cgcagcctct gtggcttagg gccgacgtgc tctacggctc
     1681 gcggtcggct cggtaggagc cgaagacgcg gagccgtgcg gtcagctctc gcagctcgcc
     1741 gagcgtcgca cccacttcgg ggtcgagcgg gtgaccgctg agcaccgcgt cgaagcgata
     1801 gcgccatgga gtcgagggaa taggccgcga cgcgagcaac gacaggtcga tgtcgtgccg
     1861 cgcgagaggc tcgagggcgc ggtacagcgc gccgggctgg tggttcgtga cgaacgagaa
     1921 ggccgtccgc cactcgccgt ctcggcgatc gagccgcgtg tacggcgcga tcgagacgaa
     1981 gcgtgtgtat gcctccggat ggtcaccgac gtcctcctcc accaccacga gcttgttgag
     2041 ggcggctgct cggtgactgg cgatggccac gacacccgag tcgccgatct cggcgacctg
     2101 tcccgccgcg tcgcccgtcg tcggcgaggc agttgcagtc gcccacggca tcgacgccag
     2161 caaggcgcgg cactgatcga gggcgaccgg gtgcgagtgg atctcgcgga tctccgttcg
     2221 ctccactgct tccgggccga cgagacaatg ccggatcggc aggatcgcct cggcgacgat
     2281 cgagagggcc gagtcgtaga gcaggtcgtg cgtctcggcg accgggccgg agagcgagct
     2341 ctcgatcggc agcacgccgt acggcgtcga gcccgacgtg accgaagcga ccacgtcggc
     2401 gaacgacggc agggggatca ggcgggcggc cgggaaaagg cggtcgcaag cggccgcgct
     2461 gtgggctcct tcgcgaccgg gataagcgac ggtcagctct gctgtcatca tcatctggct
     2521 tccacctctc gagaacgaac gacgtctccg ccggagttgt cagctagccg gcgcgaggcg
     2581 gaacgacgga gcgccggcta gcgcctaaag gcgaagccga agaacgcgaa gccgaggaat
     2641 gatggatgcc gcgcgctcgt catacgccta cacggtaggt gaacgctcga ggctgcgcaa
     2701 gacgtggtcg ccgaacgacg cagtcgacgc cgagccgccg agatcggcgg tcggctcctc
     2761 cgccagcgca cgatcgacgg cgacgtcgag agcctcggcg tccgtggggc ggccgaggcc
     2821 ctgcgcgagc agtagcgcgg tcgagcggag catcgccgca ggattcgcga ccccctgtcc
     2881 cgcgatctgc ggcgcagagc cgtgcacggg ctcgaagatc cccggcccgt cgtcaccgag
     2941 cgaggccgag gccgccaggc cgagtcctcc ggtcaccgcg gccgcgacgt cggagaggat
     3001 gtcgccgaag gtgttctcgg tcaccaccac gtcgatgctc tggggcgcct ggacgatcac
     3061 catcgcgaac gagtccacga gcgcgtggtc gagtcgcacg tcgggatact cggatgccag
     3121 ctccgtcacc acgtctcgcc agaggcgaga ggtatggaga acgttgacct tgtcgaccga
     3181 tgtcagacga cccgaccgtg cctgcgcggc ttcgaagccg cggcgggcga tccgctcgac
     3241 ctccgggcgc gagtactcgc aggtgtcgaa ccaggttccg tcgggacgcg tgcctttctc
     3301 gccgaagtag aggccccccg tcagctcgcg cacgatcatg aggtcgatgc cggccgcgcg
     3361 agccggtcga atgttggcgt agacgccgag ctccttgcgc agcgcgagca agccttgctc
     3421 cggccggacc ggcttccctt ccagctccgg cagtccgacg gctccgacca gcaccgcgtc
     3481 cgcctcacgg caggcggcga gggtctcctc gggcaacggc ggcgcgcccg ccaggatcgc
     3541 accggcaccg aacgggtgct cggagtgctc gatgccgagg gcgtccagga cgcgaacggc
     3601 ctgctcggca acctcggggc cgatcccgtc cccggggagt acggcaacct tcacgagacg
     3661 gcgtcggctg gactagctcc gtggggcgcc cggctcacgc cgcgagcatc tcgtcgacga
     3721 agacgtcggc gtcggcctcg atctcgatcg gagtgaacgt gtgcgcgacc gcctgcggcg
     3781 tcttgagtcc gtcaccggtc acgagcagga ccacccgtga gtcgcggccg acgcgcccgt
     3841 cggcgaccgc cgcccgcagc gccccgagag tgacaccgga cgcggtctcg ccgaagacgc
     3901 cggtcgtgcc ggccagcgag gccatgttct cgccgatctc gtcctccgcc accgtgtaga
     3961 tgccgccgcc cgtctcgcgg gcggtcgtca cggcgaagtc tccgtctgcc gggctgccga
     4021 tcgcgagcga gcgggccagc gtgtcgggcc ggacgggctg gacgttgcgg ccctcgcgga
     4081 acgcaagcgc gacgggctcg cacccttctg cctgcccgcc gatcagcctc ggcgtgtcgc
     4141 cgtccacgag cccgagcgcc tggaactcct tgaagccctg cccgaccttg tggaacagcg
     4201 caccggacgc gatcggaccg accacggcat cgggcgtctc ccagccgagt tgctcggcca
     4261 cctcgaacgc gatcgtcttc gatccttctg cgtagtacga ccgaaggttg acgttgacga
     4321 agccccacgg cagctcgaag gagagctcca ccgagagccg tgagcaatgg tcgtacgtcc
     4381 cgttcacggc gtagaggcga gccccgtacg cggcggccga ggcaagtttt tcgggctcga
     4441 ggtcggcggg acagaacacg gccgcctcga ggccctccgc ggcggcacgg gcggccacgg
     4501 ctcccgccag gttccccgtc gacgagcacg agagcgtctc gaaaccgagc tcctgcgctt
     4561 tccgcgcggc gacggcgacg acacgatcct tgaacgagtg cgtggggttg gcggtatcga
     4621 gcttcagcca gagctcgccg acgccgacct ccgccgcgag ccgcggtgcg gggacgagtg
     4681 gtgtcagtcc cggcgggatg agctgttgct cgggcgcctc gac
//
LOCUS       DBNGNK010000161         8478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_527964,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000161 DBNGNK010000000
VERSION     DBNGNK010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_527964"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="AC150V_04665"
     CDS             complement(<1..604)
                     /locus_tag="AC150V_04665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762248.1"
                     /translation="MAHSKETPLAGRVIAVAGAAGNLGPTVVRRLAAVGATLGLSGRN
                     VEPLEALAGELGGDTDVAAVDFLDPDATCVWADGLAERFGHVDGLVHLVGGWCGGTPI
                     EQAPLTDWDLLHDLLIRTLQHTTRAFYPHLSESEHGRVVVISSSQAQAPTHGNAAYAA
                     AKAAADAWTLALADRFRGTGSTANLIVVGAILTPEMRDAEP"
     gene            complement(608..1204)
                     /gene="leuD"
                     /locus_tag="AC150V_04670"
     CDS             complement(608..1204)
                     /gene="leuD"
                     /locus_tag="AC150V_04670"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007328384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR3762249.1"
                     /translation="MKPFRKTTGRIAVLDRHNVDTDQIIPKQFLKRIDRTGYGDALFF
                     DWRYEENGETPRAEFELNHPQFEGASILITGRNFGCGSSREHAVWALQDYGFDVVIAP
                     SFGDIFHSNAGKAGLLTVTLPEEEVKLLMELVDLDAGSELHVDLERLIVVDPNGREIV
                     FEHDTSMRHRLLHGLDDISLTLEHEDEIAAYEAAHGIA"
     gene            complement(1201..2604)
                     /gene="leuC"
                     /locus_tag="AC150V_04675"
     CDS             complement(1201..2604)
                     /gene="leuC"
                     /locus_tag="AC150V_04675"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR3762250.1"
                     /translation="MAKTMFAKIWDEHVVHEVEGEPALIYIDLHLIHEVTSAQAFEGL
                     RLAGRGVRRPALTVGTMDHNVPTTDGPIRDPMARAQLDALRRNCEEFGVELHATGSGR
                     EGIVHVIGPELGLTQPGTTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLHQRR
                     PRSMLLEFEGELHPGITPKDMILGAIGVLGVHGGVGYVVEYAGESIREMSMEGRMTVC
                     NMTIEWGARAGMIAPDDTTFAYLAGRPHAPAGAEWERVLERWRELPSDEGAFYDRVER
                     IDVAELAPQVTWGTNPGMVVPVTGAVPHPSDFDDPDDRAATERALEYMSLEPGTPIED
                     ITIDKVFIGSCTNARLDDLREAAAIVAGRRLAPGVSGLVVPGSARVRVEAEEEGLDRV
                     FTEAGFEWRRAGCSMCLGMNPDALSPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAA
                     AAVEGHFVDVRKLEVAV"
     gene            2652..3227
                     /locus_tag="AC150V_04680"
     CDS             2652..3227
                     /locus_tag="AC150V_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431569.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HIR3762251.1"
                     /translation="MSSVDRTLTWLRVPAEDDLPADVLELWQAPLEKLGFVPNVLRLF
                     ALRPSHLMAWNDHYEELMKGDSGLTKAEREMIAVVVSVANRCEYCIAAHSAALRKLTK
                     DPALADSIAADHTSAPVEPRVRAMLDYAVKLTRQPVEMTEADVEALRDAGWTDEDVMD
                     IAEVTGMFNFSNRLASGLGWAPNPEYESLGR"
     gene            complement(3232..4404)
                     /locus_tag="AC150V_04685"
     CDS             complement(3232..4404)
                     /locus_tag="AC150V_04685"
                     /inference="COORDINATES: protein motif:HMM:NF018485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762252.1"
                     /translation="MSPARPGTVLAGAVAAQTAVAFLGFGLPAIGPSLRDEFDLGLPA
                     LGALLSTGVLGAGIALIPAGWLVDRVGTRGAVLAGTGPGVVALAVAALADSAVAVFLA
                     LLIAGIGSAVVPVGGTGALFRVYGPERRGWALGVRQTAVPLGGIVGAATLPLLVRAGG
                     TELVFGLAAALVGLTGVWFALVAPRDHAPLRAGLAVRKLWRTPDIRRLVLVAGCFIIV
                     LQAVLVYLVPAVRDAGHSAFVAGTAFVILNITAVLARIVWGRIADRGGGQRRVRTLVD
                     VGLVAAAGAVVFTVALHAGPVATLAGTIVFGAGALGWNAVVYVTAGELVRPELAGQAF
                     AFVATIVFVMPAFAAPPAGALAAAAGWDVLWLTTAGVAVLGAWLASGLKRGAAVTD"
     gene            4428..4844
                     /locus_tag="AC150V_04690"
     CDS             4428..4844
                     /locus_tag="AC150V_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762253.1"
                     /translation="MERSEIARAVRRRQALEAIEEERERERVLAEQLDEIVAEADGPA
                     VDAVVFETMEPADVRLLRELFGEDLPDSRDELESVDFFEEVDEEGESVEEEIVRLQTE
                     VRLCSRRVRALERYIQGLEAVTAAAEPREEERATGG"
     gene            4875..5327
                     /locus_tag="AC150V_04695"
     CDS             4875..5327
                     /locus_tag="AC150V_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762254.1"
                     /translation="MALALALLAAGVGAAVVFAGNWLVGGPSKESYIAKVNEICDFYG
                     TQLDSVPPPDDSAPGAIFEAVDTVIPILENILNEVRGTKAPDDIQPEVDRFIELSGIS
                     IAQLRLVRLEAVERDLYGAALAFTRFEETRNKAQAVGRRLGFKCSVGL"
     gene            complement(5305..6072)
                     /locus_tag="AC150V_04700"
     CDS             complement(5305..6072)
                     /locus_tag="AC150V_04700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3762255.1"
                     /translation="MIVDALTFLGDSLFGVRAEPTELLAKMDETGVDRAIVCPLKPRG
                     YHLNEANDAVAAAVELHPDRLVGFARVDPWRGEDAVIELRRAVESSGLRGLFLHPWEE
                     TFRVSDPVTDPVVAVARELGLPVIVAAGYPWLSEALQIAELAGRHPGVSFVATNGGQL
                     NVSGLGQTDAELALESCPSLALQTAGVYREDFIEGVVSRFGPERVLFASGFPFLEPRL
                     EILRVQWAPGLDDTSRDAILGGNASRLLGAATGRPST"
     gene            complement(6069..6821)
                     /locus_tag="AC150V_04705"
     CDS             complement(6069..6821)
                     /locus_tag="AC150V_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3762256.1"
                     /translation="MIELVIIDAHMHVDHLPALGWEMGADVCVRRMDEAGIETGVVMT
                     IVDAPEVNPGAIELIAEALAAHPGRLEAFARIHPWYGDAAVALLERAITEFGFKGLKL
                     HPVTTIAHPAHEDSLRLIRAAAAHDAPTLVHCGDEPLTTPLAIARAADACPEATIILG
                     HMGGYFHVDEAIETAERHPNLVFDTSAMPYPAKIREAVERIGAERVLYASDGPFCSPR
                     IEVEKVRLAGLTPADERLVLAENTRRLLAAVR"
     gene            6820..7254
                     /locus_tag="AC150V_04710"
     CDS             6820..7254
                     /locus_tag="AC150V_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3762257.1"
                     /translation="MNEIEEVLAELLADTAASRVTLRRDVPGEVAFPVTDEVLAPGAP
                     SLRDERTVDLRTQPVAVEVRAGRQVVQDDCAAAYDDPAFQRMLGVYGGLAAQIVTPVL
                     LGGEVRGIVSLHQLERTRRWTDDEIAAASRTAERVGELLEEQ"
     gene            7251..8282
                     /locus_tag="AC150V_04715"
     CDS             7251..8282
                     /locus_tag="AC150V_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR3762258.1"
                     /translation="MRIKADSGHNRWHPDLEPVERVDPGAEIVFETRDGIDGQLTSES
                     RHEDAAQLDLGLGHPLTGPVYVEGAEPGDVLDVELLAYETEDFGVTAVIPGFGFLADE
                     FTDPFVVKWHVADGVARAKELPGVAVPGDTFAGVVGVAPSHALMEEMRGREEAIRQAG
                     GPVADSLPERAHPPEAGPGLRTIPPRETGGNMDVRQLVAGSRLFLPVEVPGALFSIGD
                     LHFAQGDGEACGTGIEIAGSATVRLNLQKQPTWRPRFPAYETPERPGRRSFATTGIPV
                     NDAMDLSAATREALREMIGWLEATHDLPRPAAYALCSVCVDLRLSEIVDVPYPLVSAL
                     CPLDVFEHG"
     gene            8291..>8478
                     /locus_tag="AC150V_04720"
     CDS             8291..>8478
                     /locus_tag="AC150V_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762259.1"
                     /translation="MTDRLTIGGLDGTVAVVTGGASGIGEACVRLLERSGAQAVIVDL
                     AGDQPIDVTDRAALAAVAG"
BASE COUNT         1293 a         2904 c         3043 g         1238 t
ORIGIN      
        1 cgggctcggc gtcgcgcatc tccggagtga ggatcgcacc cacgacgatg aggttcgccg
       61 tcgagccggt gccgcggaac cgatctgcga gcgcgagcgt ccatgcgtcg gcggcggcct
      121 tcgcggcggc gtacgcggcg tttccgtggg tcggagcctg ggcctggctc gacgagatca
      181 cgacgacgcg cccgtgctcg ctctcgctca ggtgcgggta gaaagcgcgc gtcgtgtgct
      241 gcagcgtgcg gatgaggagg tcgtgcagga gatcccaatc ggtcagaggc gcctgctcga
      301 tcggagtgcc gccgcaccag ccgccgacga gatggacgag gccgtcgacg tggccgaagc
      361 gctctgcgag gccgtccgcc catacgcagg tcgcgtcggg gtcgaggaag tcgactgcgg
      421 cgacgtccgt gtcgccgccg agctcacccg ccagcgcctc gagcggctcg acgtttcggc
      481 cactcaggcc cagagtcgct cccacggccg ccaggcgccg aacgacggtg ggcccgaggt
      541 tcccggctgc gccggcgact gcgatcacgc gtcctgcgag aggggtctcc ttgctgtgcg
      601 ccacgtgtca ggcgatcccg tgcgcagcct cgtacgccgc gatctcgtcc tcgtgctcca
      661 gggtcaggct gatgtcgtcg agcccgtgga gcagccgatg gcgcatcgat gtgtcgtgct
      721 cgaagacgat ctcgcggccg ttcgggtcga cgacgatcag tcgctccagg tcgacgtgca
      781 gctcggatcc ggcgtcgagg tcgacaagct ccatcaggag cttcacctct tcctccggca
      841 gtgtcaccgt gagcaggccg gccttgccgg cgttcgagtg gaagatgtcg ccgaacgagg
      901 gggcgatcac gacgtcgaag ccgtagtcct ggagcgccca cacggcgtgc tcgcgcgacg
      961 agccgcagcc gaagttcctg ccggtgatca ggatcgacgc cccctcgaac tgcggatggt
     1021 tgagctcgaa ctcggctcgc ggcgtctcgc cgttttcctc gtagcgccag tcgaagaaga
     1081 gcgcgtcccc gtagccggta cggtcgatcc gcttgaggaa ctgcttcggg atgatctggt
     1141 cggtgtccac gttgtggcgg tcgagaaccg cgattcgtcc ggtggttttc ctgaacggct
     1201 tcacaccgcc acctcgagtt tgcgcacgtc cacgaaatgc ccttcgaccg ccgccgcggc
     1261 ggccatcgcc gggctgacga ggtgcgtgcg ccccccgcgc ccctggcggc cctcgaagtt
     1321 gcggttcgag gtggaggcac agcgttctcc gggggagagg gcgtcggggt tcatgcccag
     1381 gcacatcgag cagccggcgc gtcgccactc gaagcccgcc tcggtgaaga cgcgatccaa
     1441 gccctcctct tccgcctcca cccgcacgcg cgcggagccg gggacgacca gcccgctcac
     1501 gcccggcgcg aggcgccggc cggcgacgat cgccgcggcc tctcggaggt cgtccagccg
     1561 tgcgttcgtg cacgagccga tgaacacctt gtcgatcgtg atgtcctcga tcggcgtccc
     1621 gggttcgagg ctcatgtact cgagcgcccg ctccgtcgcc gcacggtcgt ccgggtcgtc
     1681 gaaatcggaa gggtgtggaa cggcgccggt aaccgggacg accatgccgg ggttggtacc
     1741 ccaggtcacc tgaggggcga gctcggccac gtcgattctc tccacccggt cgtagaacgc
     1801 accctcgtcg gagggcagct cccgccagcg ctcgagcacg cgctcccact cggcgccggc
     1861 cggtgcgtgg ggtcggcccg cgaggtacgc gaacgtcgtg tcgtcgggag cgatcatccc
     1921 cgcgcgcgcg ccccactcga tcgtcatgtt gcagaccgtc atccgccctt ccatcgacat
     1981 ctcccggatc gactcgcccg cgtactcgac gacgtagccg acgccgccgt ggacgccgag
     2041 cacgccgatc gccccgagga tcatgtcctt gggggtgata cccgggtgca gctcaccctc
     2101 gaactcgagc agcatcgacc tcggacggcg ctggtgcagc gtctgggtcg ccagcacgtg
     2161 ctcgacctcg gatgtcccga tgccgaacgc gagagcgccg aacgcgccgt gggtcgaggt
     2221 gtgcgagtcg ccgcagacga tcgtcgtgcc cggctgagtc aggccgagct cgggcccgat
     2281 gacgtgcacg atcccctcgc gtcccgatcc ggttgcgtgg agctcgacgc cgaactcctc
     2341 gcagttccgc cgcagcgcgt cgagctgagc gcgcgccatc gggtcacgga tgggaccgtc
     2401 cgtggtgggg acgttgtgat ccatcgtccc gaccgtgaga gcgggccggc ggacgccgcg
     2461 ccctgcgagc cggaggccct cgaaagcttg agcggacgtc acctcgtgga tgagatggag
     2521 gtcgatgtag atgagcgccg gctcaccctc cacctcgtgc acgacgtgct cgtcccagat
     2581 cttcgcgaac atcgttttgg ccacgtctcg atgatacggc gtgtcgtgcc gctcacgttt
     2641 aggatcgccg catgtcgtcc gtcgaccgca cgttgacctg gcttcgtgtg cccgcggagg
     2701 acgacctgcc ggccgacgtt ctcgagctct ggcaggcgcc gctcgagaag ctcggcttcg
     2761 tcccaaacgt gctccggctc tttgcactgc ggccctctca cctcatggcc tggaacgacc
     2821 actacgagga gctgatgaag ggcgactccg ggctgacgaa ggccgagcgg gagatgatcg
     2881 ccgtcgtcgt ctcagtcgcc aaccgctgcg agtactgcat tgctgcgcac tccgccgccc
     2941 tccgcaagct gacgaaggat ccggcgctgg ccgactcgat cgcggccgat cacacgtcgg
     3001 cgcccgtcga gcccagagtg cgcgccatgc tggactacgc ggtgaaactg acgcgacagc
     3061 ccgtggagat gacggaggcc gatgtcgagg cattgcgcga cgcagggtgg acggacgagg
     3121 acgtgatgga catcgcggag gtgaccggga tgttcaactt ctcgaaccgg ctggcgagcg
     3181 gcctcggctg ggcgccgaat cccgagtacg agagcctcgg gcgctgatcg gctagtcggt
     3241 cacggcagcg ccgcgtttca gccctgacgc gagccacgcg cccagcacgg cgacgcccgc
     3301 cgtggtcagc cagaggacgt cccagccggc ggcggcggcg agcgctcccg caggcggcgc
     3361 ggcgaaggcc ggcatgacga acacgatcgt cgcgacgaac gcgaacgcct gaccggccag
     3421 ctcgggccgt acgagctcgc cggccgtgac gtagacgacc gcgttccaac cgagcgcgcc
     3481 tgcgccgaag acgatcgtgc ccgcgagcgt tgcgaccggg ccggcgtgga gcgcgacggt
     3541 gaagacgacg gccccggccg cagcgacgag acccacgtcc acgagcgtgc gcactctccg
     3601 ttggccgccg ccgcggtcgg cgatccgccc ccagacgatc cgggccaaga cggccgtgat
     3661 gttcaggatc acgaatgccg tgccggcgac gaaggccgag tggccggcgt ctcggaccgc
     3721 cgggacgagg tagacgagga cggcctgcag gacgatgatg aagcagccgg cgacgagcac
     3781 gagtctccga atgtccggcg tgcgccagag cttgcggacg gcgaggccgg cgcggagcgg
     3841 cgcatggtcg cgcggagcga cgagcgcgaa ccacacccct gtgaggccga cgagcgccgc
     3901 ggccaagccg aagaccagct ccgtaccgcc tgcccgtacg agcaagggca gcgtggcggc
     3961 gccgacgatc ccgccgagcg gaaccgccgt ctgccgtacg ccgagcgccc agccgcggcg
     4021 ctcgggcccg tacacgcgga agagcgcgcc ggtgcctccg acggggacga ccgccgatcc
     4081 gatcccggcg atcaggagcg cgaggaagac ggccacggct gagtcggcaa gggccgcgac
     4141 cgccagcgcg accacgcccg gcccggtgcc tgccaggacg gcgccgcgcg tgccgacgcg
     4201 gtcgacgagc caccctgccg ggatcaacgc gatgccggct ccgagcaccc cggtactgag
     4261 cagcgcgccc agcgcgggca ggccgagatc gaactcgtcc cgcaggctgg ggccgatcgc
     4321 cggcaggccg aagccgagaa acgcaacggc ggtctgagcg gcgaccgctc ctgccagaac
     4381 agtgccgggt ctggctgggc tcatgatcgc ttgtctatcg ttccctcgtg gagcgaagcg
     4441 agatcgccag agcagtccgt cgccgccagg ccctcgaggc aatcgaagag gagcgggagc
     4501 gcgagcgcgt gctcgcggag cagctggacg agatcgtcgc cgaggccgat gggccagccg
     4561 tcgacgccgt cgtcttcgag accatggagc cggcggacgt caggctgctg cgggagctct
     4621 tcggcgagga cctgcccgac tcccgcgacg agctggagtc tgtcgacttc ttcgaggaag
     4681 ttgatgagga gggggagtcg gtcgaggaag agatcgtgcg gctccagacc gaggtccggc
     4741 tctgcagtcg gcgcgtgcgg gcgctcgaac gctacatcca ggggctcgaa gccgtcacgg
     4801 ccgcggcaga gcctcgcgag gaggagcgcg ccacaggagg ctgatcgttg cgttccaggc
     4861 tctctcgtat cgggatggct ctcgctctcg ccctccttgc agcgggggtc ggcgcggcgg
     4921 tcgtcttcgc cggaaactgg ctggtcggcg ggccgagcaa ggagtcgtac atcgcgaagg
     4981 tgaacgagat ctgtgacttc tacggcacac aactcgacag cgttcctccc cccgacgact
     5041 cggctcccgg cgcgatcttc gaggccgtcg acacggtgat cccgattctc gagaacattc
     5101 tcaacgaggt gcgtgggacg aaggcgcccg acgacatcca gcccgaggtc gaccgcttca
     5161 tcgagctgag cgggatctcg atcgcgcagc tccgcctcgt ccgactcgag gccgtcgagc
     5221 gcgacctcta cggcgcggcc ctggccttca cccgtttcga ggagacgcgc aacaaggcgc
     5281 aggccgtcgg ccggcgtctt ggcttcaagt gctcggtcgg cctgtagccg cgcccagaag
     5341 gcgcgaggcg ttgccgccga gtattgcgtc gcgcgaggtg tcgtccagcc caggggccca
     5401 ctggacgcgg aggatctcga gacgtggctc gaggaaggga aagccggacg cgaacagcac
     5461 tcgctccggc ccgaaccgcg agacgacgcc ttcgatgaag tcctcccggt agacgcccgc
     5521 ggtctggagg gcgaggctcg ggcagctctc gagagcgagc tccgcatccg tttgtcccag
     5581 ccccgagacg ttgagctgac cgccgttggt cgccacgaag ctcacgccgg ggtgccgccc
     5641 ggcgagctcc gcgatctgga gcgcctccga aagccatggg tacccggccg cgacgatgac
     5701 agggaggccg agctcgcgcg cgacggcgac cacggggtcg gtgacggggt cggacacccg
     5761 gaaggtctcc tcccatgggt ggaggaagag gccacgcagg ccgctgctct ccaccgcgcg
     5821 ccgaagctcg atgaccgcgt cctctccgcg ccaggggtcg acgcgcgcga agccgaccag
     5881 cctgtcgggg tggagctcga ccgcggctgc gaccgcgtcg ttcgcctcgt tcaggtgata
     5941 gccgcgtggc ttcagcgggc agacgatcgc gcgatcgacg ccggtctcgt ccatcttcgc
     6001 gagcagctcc gtcggctcgg cccgaacgcc gaagagcgag tcgccgagaa aggtgagcgc
     6061 gtcgacgatc atcgcaccgc ggccagcaag cggcgggtgt tctcggcaag cacgagccgt
     6121 tcgtcggccg gggtcagccc ggcgaggcgc accttctcca cctcgatgcg tggcgagcag
     6181 aagggcccgt cgctcgcgta caggacacgc tcggctccga tgcgctcgac tgcctcgcgg
     6241 atcttcgccg ggtagggcat cgccgaggtg tcgaagacga gattcgggtg ccgctcggcc
     6301 gtctcgatcg cctcgtccac gtgaaagtag ccgcccatgt gtccgaggat gatcgttgcc
     6361 tcggggcagg cgtccgccgc gcgcgcgatc gcgagcggcg tcgtcagtgg ctcgtcgcca
     6421 cagtgcacga gcgtgggggc gtcgtgtgcc gcggccgcgc ggatcaggcg aagcgagtcc
     6481 tcgtgcgcgg ggtgcgcgat cgtggtcacg ggatgcagct tgagcccctt gaagccgaat
     6541 tcggtgatcg cgcgctcgag cagggcaacg gccgcgtcgc cgtaccacgg gtggatccgg
     6601 gcaaacgctt cgaggcgacc cgggtgggcg gcgagcgctt cggcaatcag ctcgatcgcg
     6661 ccggggttga cctcgggcgc gtcgacgatc gtcatcacga cgcccgtctc gatgccggcc
     6721 tcgtccatcc gccggacgca aacatcggcc cccatctccc agccgagcgc cggcaggtgg
     6781 tcaacgtgca tgtgggcgtc gataatcacg agttcgatca tgaacgaaat cgaagaagtc
     6841 ctggccgaac tgctcgcgga cactgccgcc agccgagtga cgctgcggcg ggacgtcccc
     6901 ggcgaggtcg cgttcccggt gaccgacgag gtgctcgctc cgggagcgcc ctccttgcgc
     6961 gacgagcgga cggtcgacct tcgtacccag ccggtcgcgg tcgaggtgcg ggccggccgg
     7021 caggtcgtgc aggacgactg cgccgccgcg tacgacgatc cggccttcca gcggatgctc
     7081 ggcgtctacg gtgggctggc ggcccagatc gtcacgccgg tcctgctcgg cggcgaggta
     7141 cgggggatcg tctcgctgca ccagctcgag cggacgaggc ggtggaccga cgacgagatc
     7201 gcggccgcgt cgcgcacggc cgagcgcgtc ggcgagttgc tggaggagca atgaggatca
     7261 aggccgacag cggtcacaac cgctggcacc cggacctcga gccggtcgag cgggtagacc
     7321 ccggagcgga gatcgtcttc gagacgcgtg acgggatcga cgggcagctc acgtccgaga
     7381 gccggcacga ggacgcggcg caactggatc tggggctcgg ccatccgctc acggggccgg
     7441 tgtacgtcga aggcgccgag ccgggcgacg tgctggacgt cgagctcctc gcctacgaga
     7501 cggaggattt cggcgtcacg gcagtgattc ccggcttcgg cttcctggcg gacgagttca
     7561 ccgacccgtt cgtcgtcaag tggcacgtcg ccgacggggt cgcgcgggcg aaggagcttc
     7621 cgggagtggc ggtgcccggc gacaccttcg cgggtgtcgt cggcgtcgct ccgtcacacg
     7681 ctctgatgga ggagatgcgt gggcgggagg aggcgatccg ccaggcagga ggcccggtcg
     7741 ccgactcgct gccggagcgt gcgcatccgc cggaggcggg gcccgggctg cggacgattc
     7801 ccccacgcga gacgggcggc aacatggacg ttcgccagct cgtcgccgga tcccgtttgt
     7861 tcctgcccgt cgaggtgccg ggcgccctgt tctcgatcgg cgatctgcac ttcgcgcagg
     7921 gcgacggcga ggcctgcggt acgggaatcg agatcgcggg ctcagcgacc gtccggctga
     7981 atctccagaa gcaacccacc tggcgtccgc gcttcccggc ctacgagacg ccggagcggc
     8041 ccggacgccg ctcgttcgcg acgaccggga tcccggtcaa cgacgcgatg gacctgagcg
     8101 cggccactcg ggaggcgctg cgggagatga tcggctggct cgaggcgacc cacgatctcc
     8161 cgcggccggc cgcgtacgcg ctctgctcgg tgtgcgtcga cctgcggctg tcggagatcg
     8221 tcgacgtccc gtacccgctc gtctcggcgc tctgccccct cgacgtcttc gaacacggat
     8281 aggaggagcg atgacggatc ggctcacgat cggcggcttg gacggcacag tggccgtcgt
     8341 cacgggcggg gcctcgggca tcggcgaggc gtgcgtgcga ctgctcgagc gcagcggcgc
     8401 ccaggccgtc atcgtcgatc tcgccggcga ccagccgatc gacgtgaccg atcgcgccgc
     8461 gctcgccgcg gtcgctgg
//
LOCUS       DBNGNK010000162         1855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_671817,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000162 DBNGNK010000000
VERSION     DBNGNK010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_671817"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC150V_04725"
     CDS             <1..322
                     /locus_tag="AC150V_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762260.1"
                     /translation="ASVGDRGFAAYEREHARRTTWIVAPLMVAEALAAGTIVVLDPGL
                     AAAAGAALVVGIWLSTAFVQVPCHRRLAAGWDAAAQRRLVRTNWARVGMWSARASIAI
                     LLLV"
     gene            517..1605
                     /gene="rsgA"
                     /locus_tag="AC150V_04730"
     CDS             517..1605
                     /gene="rsgA"
                     /locus_tag="AC150V_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR3762261.1"
                     /translation="MHDLQTLGWNDRLSGQFEQYRDDGLVPGRVSVQHRGAWDVLTEQ
                     GELRCELPGRVFHEAGSAAELPAVGDWVAVEPRAGEASGTIRALLPRRSKFSRLAAHD
                     PASNVTREQVLASNVDVVFIVTSLNEDLNLRRLERYVTLAWESGATPVILLTKSDLHP
                     APEEAAAEVESIAYGVPVHTLSALTGRGLDVIAEHLQPGSTAVLLGSSGVGKSTLINA
                     LIGEEVLGTQEIRSDGRGRHTTTRRELLVLPSGGIVIDTPGIRELQLWEADEGLDEAF
                     EDIAELAARCRFTDCAHDSEPDCAVRDALENGTLAPDRWESYQKLERELAHLDRRLDK
                     RAEAEERKRWRAMSVDARRRYRERGRPR"
     gene            complement(1624..>1855)
                     /locus_tag="AC150V_04735"
     CDS             complement(1624..>1855)
                     /locus_tag="AC150V_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762262.1"
                     /translation="DGALLDALSYEGEITAATIDGRIYSLVEGTALAAADSNTVDGSL
                     ARLPDGADTDDAASDWSFSTTPTPGAANVATP"
BASE COUNT          274 a          639 c          656 g          286 t
ORIGIN      
        1 cgcgtctgtc ggcgaccgcg gctttgccgc gtacgagcgt gagcacgctc gccggacgac
       61 ctggatcgtc gctcctctga tggtcgccga agcgctcgcg gcggggacga tcgtcgtcct
      121 cgaccccggg ctggcagctg ccgccggggc ggctctggtc gtcggcatct ggctctcgac
      181 cgcgttcgtc caggttccgt gccaccggcg gctggcggcc ggctgggacg cagccgccca
      241 gcgcagactc gtccggacga actgggcgcg cgtgggaatg tggagcgcac gcgcgtcgat
      301 cgccattctg ctgctcgtct gagatactgc gaccgtcccc gtccgcagga ggtggtgtgc
      361 cgatgaccga tctcgcagct tccttcgcac cgaccgccgc gaggcgccgg gcgtagcggg
      421 caccaccggc tcgtcgagct cccgaacggc ctcgcgcact ggcgcgccga gcggcgcgtc
      481 gctttcatgt actcgagtcc aagggagacg agcgcagtgc acgatcttca aacgctcggc
      541 tggaacgacc ggctgagcgg gcaattcgag cagtaccgtg acgacggtct cgttccgggc
      601 cgcgtcagtg tccagcaccg cggcgcgtgg gacgtcctca ccgagcaggg agagttgcgt
      661 tgcgagcttc ccggccgcgt gttccacgag gccggcagcg cagccgagct ccccgcggtc
      721 ggcgactggg tcgcggtcga gccgcgagcc ggcgaggcga gcgggacgat ccgcgcgctg
      781 cttccgcgcc ggtcgaagtt ctcgcggctc gcggcgcacg acccggcttc caacgtcacg
      841 cgcgagcaag tcttggcatc gaacgtcgac gtcgtgttca tcgtcacctc gctcaacgag
      901 gatctcaatc tccgccgcct cgagcggtat gtgactctcg cctgggagag cggcgctaca
      961 cctgtgatcc tgctcacgaa gtccgacctc cacccggctc ccgaggaggc cgcggcggag
     1021 gtcgagtcga tcgcttacgg cgtcccggtg catacgctgt ccgcgctgac ggggcggggc
     1081 ctcgacgtga tcgcagaaca cctgcagcct ggttcgactg ccgtcctgct cggctcgtcg
     1141 ggcgtaggga agtcgacact gatcaacgcg ctcatcgggg aagaggtgct cggcacccag
     1201 gagatccgga gcgacgggcg cggacgccac accaccacgc gccgcgagct cctcgtcctg
     1261 ccttccggag gcatcgtgat cgacacgccg ggcatccgcg agctccagct gtgggaagcc
     1321 gacgagggac tcgacgaggc gttcgaggac atcgccgagc tggccgcacg gtgccgtttc
     1381 accgattgcg cacacgactc cgagccggac tgcgccgtac gcgacgccct cgagaacggc
     1441 acgctcgcgc cggatcgatg ggagagctac cagaagctgg agcgggagct cgctcacctc
     1501 gatcgccggc tcgacaagcg tgctgaagca gaggagcgaa aacgttggcg cgcaatgagc
     1561 gtcgatgctc gccggcgcta cagggagagg gggcggcccc gctaggccac cccctcgggg
     1621 gtctcagggc gtcgcgacgt tggcagcgcc cggggtgggc gtcgtgctga acgaccagtc
     1681 gctcgcggcg tcgtccgtgt cggctccgtc aggcagccga gcaagcgagc cgtcgacggt
     1741 gttggagtcg gctgcagcga gcgccgtccc ttcgacgagc gagtagatcc gtccgtcgat
     1801 ggtggccgct gtgatctcgc cctcgtacga gagcgcgtcc agcaacgctc cgtcg
//
LOCUS       DBNGNK010000163         3269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1961979,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000163 DBNGNK010000000
VERSION     DBNGNK010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1961979"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="AC150V_04740"
     CDS             complement(<1..650)
                     /locus_tag="AC150V_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762263.1"
                     /translation="MKTAERTRIAFQRGTPDRVPIHCWLGLPLIKKLREPDQSFLDLF
                     QLWIDDPMGSIVKMQQDLGLDPMITTYSQHIGEHEIWPRMLFPRPFETDTWVETFEVT
                     SQGDGWREHAHKIRTPDGDLDYSYRTEDGFGTSCHDFLLKGDEPEKKLPALRHFPSAD
                     LYDMTILKSMTEKVGDDAWWLHHVIGPWDMAAEVRGLVDLSMDIYDRPQFVHDLMR"
     gene            957..1730
                     /locus_tag="AC150V_04745"
     CDS             957..1730
                     /locus_tag="AC150V_04745"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3762264.1"
                     /translation="MAKVEEGSQSIDRAARILVRLVESDDAVTLNGVMAETSLPKTTA
                     ARLLRALERNGLAERKQGGGFRPGPVLVDYARRDASVGDLGTIAWPFLEQLGARTGET
                     TNIGIPTPGGVARLAQVDSSHPLGAGNWIGRRIPLHASSMGKVFMAFGAAQPPFGRLA
                     KLGPATITALGDLLAELEDVRAVGYATTWDELTEGLCSTAAPVRGARETVIAAISVSA
                     PTVRMSAARLAELSAMVIEEASALSDYIQQRQQRGERIA"
     gene            1727..2392
                     /locus_tag="AC150V_04750"
     CDS             1727..2392
                     /locus_tag="AC150V_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR3762265.1"
                     /translation="MNTDEILKQLYDQCLVGNQPEVVELTNKGLELELEPTTMLFEAL
                     IPALEEVGARFERGDFFVPEMLIAGRAMAGALEILRPLMADTGAETVGTFLMGTVKGD
                     VHDIGKNLVDIMLEGAGFKVVDIGVQVAPETFVEAIQEHQPDVVGFSAFLTTTMPMFK
                     VNIEALEKAGLRDDVIVMVGGAPVTQEYADAVGADGYAPDAAAATKLAKELLEERRAL
                     TPA"
     gene            2398..2781
                     /locus_tag="AC150V_04755"
     CDS             2398..2781
                     /locus_tag="AC150V_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3762266.1"
                     /translation="MGVVTFVEHAVKKPVFGCRMCGQCVLHSTGMTCPMTCPKTLRNG
                     PCGGVREDGTCEVKPEMRCVWLKAEERARTLPLMPAPWREHFGELRPPVDNRLQGTSS
                     WLNLVTRRDRKVPGGWAGNVSETPS"
     gene            2778..>3269
                     /locus_tag="AC150V_04760"
     CDS             2778..>3269
                     /locus_tag="AC150V_04760"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3762267.1"
                     /translation="MSALGELVAAGDRLIVTGEAPTVDGGDLEAFGERVAEMGQWADA
                     MNATDNTAAHAHASNVAVAIALVRTGIEPILQVVCRDKNRLALQADIVGAALHGVENV
                     CCLTGDDVTAGDEPEARRVFDLDGPQLIRVAKTLSDGRYLSGRPLDPPLHLLIGAVEN
                     AGAP"
BASE COUNT          551 a         1079 c         1123 g          516 t
ORIGIN      
        1 atccgcatca ggtcgtgcac gaactgggga cggtcgtaga tgtccatcga caggtcgacg
       61 aggccgcgca cctccgccgc catgtcccag gggccgatca cgtggtgcag ccaccacgca
      121 tcgtcgccga ctttctcggt catgctcttg aggatcgtca tgtcgtagag gtcggcggac
      181 gggaagtggc gaagtgcggg aagcttcttc tcgggctcgt cccccttgag caggaagtcg
      241 tggcacgacg tgccgaagcc gtcctcggtg cgataggagt agtcgagatc gccgtcgggc
      301 gtgcggatct tgtgcgcatg ctcgcgccag ccgtcgccct ggctcgtgac ctcgaaggtc
      361 tcgacccagg tatccgtctc gaacgggcgt gggaagagca tccgtggcca gatctcgtgc
      421 tcgccgatgt gctggctgta cgtcgtgatc atcgggtcga ggccgagatc ctgctgcatc
      481 ttcacgatcg agcccatcgg atcgtcgatc cagagctgga acaggtcgag gaaggactgg
      541 tccggctcac gcagcttctt gatcagggga agcccgagcc agcagtggat gggcacacgg
      601 tctggcgttc cgcgctggaa ggcgatgcgg gtgcgctcgg cggtcttcac ggtgactctc
      661 ctttgtctct tcgcgctgcg gtcacgataa ccggcgcgag ggtgcgccga gaggactgtg
      721 cgtttcgcgc cggttctcgg gcggaaccgg gcttccgagc gacgcgacga tgcggcacgc
      781 gaattgcgat ccgccacctc acgagaccga gagcggcggc ggcgtcactc ggcccgtcgc
      841 agtaaggcta atctgtccta tagtcccatc aattggtacc atcgtccaac agtatcgcag
      901 tgaaaagcgc tttgcgcttg acattctgcc gaagttggta ccatcatctg gtacctatgg
      961 cgaaggtcga agaaggctca cagtcgatcg accgagcagc tcgaatcctc gttcggctgg
     1021 tcgagagcga cgacgcggtg acgctgaacg gagtgatggc cgagacgagc ctgccgaaga
     1081 cgacggcagc gcggctcttg cgcgcgctcg agcgcaacgg cctcgccgag cgcaagcaag
     1141 gcggaggctt tcggcccggc cccgtgctcg tcgactacgc acgacgcgac gccagcgtcg
     1201 gcgacctcgg cacgatcgcc tggccgttcc tcgagcagct cggcgcccgc accggcgaga
     1261 cgaccaacat cggcatcccg actcccggcg gcgttgcacg gctcgcccag gtcgacagca
     1321 gccaccccct cggggcggga aactggatcg gccgccgcat cccgctgcac gcgtcgtcaa
     1381 tggggaaggt gttcatggcc ttcggagccg cgcagccacc gttcggtcgg ctggccaagc
     1441 tcggccccgc gacgatcacg gcactgggag acttgctcgc agagctcgag gacgttcgtg
     1501 ccgtcggcta cgcgacgacc tgggacgagc tcaccgaggg gctctgctcg accgctgcgc
     1561 cagtgcgcgg cgcgcgcgag acggtcatcg ccgccatctc ggtctccgcc ccgaccgtcc
     1621 gcatgtccgc ggcacgcctg gccgagctct cggcgatggt catcgaggag gcgtcggccc
     1681 tgtccgacta catccagcag cgccaacaac gaggagaacg catcgcatga acaccgacga
     1741 gatcctgaaa cagctgtacg accagtgcct ggtcgggaac cagccagagg tcgtcgagct
     1801 cacgaacaag ggcctcgagc tcgagctgga gccgaccacg atgctcttcg aggcgctgat
     1861 acccgcgctg gaagaggtcg gcgcacgctt cgagcgcggc gacttcttcg ttcccgagat
     1921 gctgatcgcc ggtcgggcaa tggcaggggc tctcgagatc ctgcgcccgc tgatggccga
     1981 caccggcgcc gagacggtcg gcacgttcct gatgggaacc gtcaagggtg acgtgcacga
     2041 catcgggaag aacctcgtcg acatcatgct cgagggcgcc ggtttcaagg tcgtcgacat
     2101 cggcgtgcag gtcgcgccag agaccttcgt cgaggcgatc caggagcacc agcccgacgt
     2161 cgtcggcttc tccgccttcc tgacgacgac gatgccgatg ttcaaggtca acatcgaggc
     2221 actcgagaag gcggggctcc gcgacgacgt gatcgtgatg gtcgggggcg cccccgtcac
     2281 gcaggagtac gccgacgcgg tcggcgccga cggctatgcg ccggatgcgg ctgcggcgac
     2341 gaagctcgcc aaagagctgc tcgaagagcg tcgcgcactc acgccggcct gagctcgatg
     2401 ggcgtcgtaa ccttcgtcga gcacgcggtc aagaagccgg tcttcggctg ccggatgtgc
     2461 ggccaatgcg tgctccacag cacgggcatg acctgcccga tgacgtgtcc caagacgctt
     2521 cgtaacgggc cctgcggcgg cgtacgggag gacggcacgt gcgaggtgaa gcccgagatg
     2581 cggtgcgtct ggctcaaggc cgaggagcgc gcgcggacac tgccgctgat gcccgcgccg
     2641 tggcgcgagc acttcggcga gctgcggccg ccggtcgaca accgtctgca ggggacgtcc
     2701 tcgtggttga acctcgtgac gcgacgcgac cgcaaggttc ccggcgggtg ggcgggcaac
     2761 gtcagcgaga cgccgtcgtg agcgcgctcg gggagctcgt cgcggcgggc gatcgcctga
     2821 tcgtgaccgg agaggctccg accgtcgacg gcggcgatct cgaggcgttc ggggaacggg
     2881 tcgccgagat ggggcagtgg gccgacgcca tgaacgcgac cgacaacacc gcagcgcacg
     2941 cgcatgcctc gaacgtcgcc gtcgcgatag cgctcgtccg caccggcatc gagccgatcc
     3001 tccaggtcgt ctgccgcgac aagaatcggc tcgcgctcca ggccgacatc gtcggggccg
     3061 ccctgcacgg ggtcgagaac gtctgctgcc tgaccggcga cgacgtcaca gccggtgacg
     3121 agcccgaagc gcgccgcgtc ttcgatctcg acgggccaca gctgatccgg gtcgcgaaga
     3181 cgctctccga cggccgctat ctgtccggcc ggccgctcga cccgcctcta cacctgctga
     3241 tcggtgcggt cgagaatgcc ggcgcgccg
//
LOCUS       DBNGNK010000164         3213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1460391,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000164 DBNGNK010000000
VERSION     DBNGNK010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1460391"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="AC150V_04765"
     CDS             <1..1059
                     /locus_tag="AC150V_04765"
                     /inference="COORDINATES: protein motif:HMM:NF047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR3762268.1"
                     /translation="RDHTQSRLDLARESVGRALELDADLAEAHLALGEYYRLGVGDRE
                     RALDEFQIVRSRQPNNGEVHVQLAALQGELGLWEAARESFLQAWELNPLQGRLPCSAG
                     GACIALRDFARALHLHDDAIRLTPDRACPYFCKAWIYLDRDGSTAAARRFLATVPAGI
                     DLEATPPINYLWVMVEIFDGRWEAALERLDAGSSEVFEFHFFYVPKALLRGQVHELRE
                     RGDLAPGEYEAARRLLQAAVDDRPGDELLRGSLGIALAGLGRGEEARRQGELGLELLG
                     EIKDERLGFRLRDLAQIHAMLGDHEKAIEYLETMLAIESFFSAELLRRDPTWATLAGH
                     AAFEAMLARHSISPAQAG"
     gene            1240..2394
                     /gene="msrB"
                     /locus_tag="AC150V_04770"
     CDS             1240..2394
                     /gene="msrB"
                     /locus_tag="AC150V_04770"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012663227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR3762269.1"
                     /translation="MRPEIPFAAVLFAALVTIACLPGRVSSEPVDIPGPVELATFAGG
                     CFWCMEPPFEGLPGVRSVVSGYTGGPEKDPTYRRVAGGRTGHTEAVQIDFNPEIISYD
                     TLLEVYWRSFDPTDGGDQFADRGSQYRPGIFFHSEEQRRTAERSRAELAASGRFAKPI
                     VVEIVPFDAFYAAEDYHQDYYRKNPAHYQAYAVGSGRKGFLARVWTDDDFDAEEVARK
                     NDSTSSEPSRVEAASPRGYTKPPDAEIRRMLTPLQYRVTQQDGTERAFSNEYWDNKEP
                     GIYVDIVSGEPLFSSRDKYRSGTGWPSFTRPLVEENIVEHEDRSLFMARTEVRSKHGD
                     SHLGHVFDDGPRPTGLRYCINSAALRFIPAAELEAQGYGEFAAAFASKTD"
     gene            complement(2391..>3213)
                     /gene="egtD"
                     /locus_tag="AC150V_04775"
     CDS             complement(2391..>3213)
                     /gene="egtD"
                     /locus_tag="AC150V_04775"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR3762270.1"
                     /translation="ELDEYYLTRTELAIMDAAVDEMAAALGPRCLVLEYGSGAGHKTE
                     VLLEALDRPVAYVPIDISCEFLSASAARLRRRFPDVEVLPVCADYTLDYPIPSTRSAP
                     RRRVVYFPGSTIGNFHHVEAVEFLAHVAEVVGPGGGLLIGIDLDKDPAVLELAYNDAS
                     GVTAEFNLNLLGRINRELGADFDLDAFRHHAVYDAGEERIEMYLVSQREQTVRLAGES
                     FAFAPGEAIFTEHSHKYGLERFARMAARAGFSVERVWTDEEALFSVQYLSAVRPG"
BASE COUNT          519 a         1136 c         1074 g          484 t
ORIGIN      
        1 cgcgaccaca cccagtcgcg cctcgacctg gctcgggagt ccgtcgggag ggctctcgag
       61 ctcgacgccg atctcgccga ggcgcacctc gcgctcgggg agtactaccg gctcggcgtc
      121 ggcgaccgcg agcgggcgct cgatgagttc cagatcgtcc gaagccggca gcccaacaac
      181 ggcgaggtcc acgttcagct ggcggccctg cagggagagc tgggcctctg ggaggccgcc
      241 cgcgagagct ttctccaggc ctgggagctc aaccccctgc agggccgcct gccctgcagc
      301 gccgggggtg cctgcatcgc tctgcgcgac ttcgcccggg cgctccacct ccacgacgac
      361 gccatccgcc tgacgccgga ccgggcctgc ccgtacttct gcaaggcgtg gatctatctg
      421 gatcgcgacg ggagcaccgc ggcggcgcgg cggttcctcg ccacggtgcc ggccggaatc
      481 gacctcgagg cgacgccacc gatcaactat ctgtgggtga tggtcgagat cttcgacggc
      541 cgctgggagg cagcgctcga gcggctggac gccgggagct cggaggtctt cgaattccac
      601 ttcttctacg tcccgaaggc actcctgcgg gggcaggtcc acgagctccg cgagcgcggc
      661 gatctggccc cgggcgagta cgaagcggcc cggagactgc tgcaggcagc cgtcgacgat
      721 cgccccggag acgaacttct gcgcggctcc ctcgggatcg ctctcgccgg tctcggccgc
      781 ggtgaggagg ctcggcgcca gggggagctc ggcctggagc tcctgggcga gatcaaggac
      841 gagcggctcg gcttccggct ccgtgacctg gcgcagattc acgccatgct gggtgatcac
      901 gagaaggcca tcgagtacct cgagacgatg ctcgcgatcg agagcttctt ttcggccgag
      961 ctattgcggc gcgatccgac ctgggcgact ctggccgggc acgccgcatt cgaggcgatg
     1021 ctcgccagac actcgatctc cccggcgcaa gcaggttgat tggcgccaac gccgcggtgt
     1081 gatcccgggc gaccaaatct cacgttttca tcacttcgga tcgccggtgc atcccggcgc
     1141 gttccgatga gcgtaaagaa acccgagacc ggcacgcacc gcgccgtgtc atcccttccg
     1201 cagcgatcgc acgccttgca gccaacgggg gattcccaca tgcgacccga gattcctttt
     1261 gccgccgtcc tcttcgccgc ccttgtcacg attgcctgcc tgccggggag agtcagctcc
     1321 gagccggtcg acattccggg tccggtcgag ctcgcgacct tcgcgggcgg ctgcttctgg
     1381 tgcatggagc cgccgttcga agggctcccg ggcgtccgct ccgtcgtctc gggctacacc
     1441 ggcggccccg agaaggaccc cacctaccgc cgcgtcgcgg gcggccgcac cggccacacc
     1501 gaggcggtgc agatcgactt caatcccgag atcatctcct acgacaccct gctcgaggtc
     1561 tactggcgca gcttcgatcc caccgacggc ggggaccagt tcgccgaccg cggcagccag
     1621 taccgtcccg gcatcttctt ccacagcgag gagcagcggc gcacggcgga gcgttcccgg
     1681 gcggagctgg cggcttccgg ccgcttcgcc aagcccatcg tcgtcgagat cgtgccgttc
     1741 gacgccttct acgcggccga ggactaccac caggactact atcgcaagaa cccggcccac
     1801 taccaggcct acgccgtcgg ctcggggcgc aaggggttcc tggcgcgcgt gtggacggac
     1861 gacgacttcg atgccgagga ggtcgccagg aagaacgact cgacgtcctc ggaaccgagc
     1921 cgcgtcgaag cggcctcccc gcgcggctac accaagccgc ccgacgccga gatccggcgg
     1981 atgctcacac cgctgcagta ccgcgtcacg cagcaggacg gcaccgagcg cgccttcagc
     2041 aacgagtact gggacaacaa ggagcccggg atctacgtcg acatcgtctc cggcgagccc
     2101 ctcttctctt cacgcgacaa gtaccggtcg ggtacgggct ggccctcctt cacccgtccc
     2161 ctggtcgagg agaacatcgt cgagcacgag gaccgctcgc tcttcatggc gcggacggag
     2221 gtgcgctcga agcacggcga ctcgcatctc ggccacgtct tcgacgacgg cccgcggccg
     2281 accgggctgc gctactgcat caactcggcg gcgctgcgct tcattccggc cgcggagctc
     2341 gaagcgcagg gatacggcga gttcgcggcc gcgttcgcct cgaagaccga ctagcctggc
     2401 cggacggcgc tcaagtactg gacgctgaag agtgcctcct cgtccgtcca gacccgctcc
     2461 accgagaaac ccgcacgcgc ggccatgcgg gcgaagcgct cgaggccgta cttgtgggag
     2521 tgctcggtga agatcgcctc gccgggcgcg aaggcgaacg actcgccggc gaggcgcacc
     2581 gtctgctcac gctggctcac caggtacatc tcgatgcgct cctcgccggc gtcgtagacc
     2641 gcgtggtgcc ggaaggcgtc gagatcgaaa tcggcgccga gctcgcggtt gatgcggccg
     2701 agcaggttca agttgaactc ggcggtgacg ccgctcgcat cgttgtaggc gagctcgagc
     2761 acggccggat ccttgtcgag atcgattccg atcaggaggc caccgcccgg accgacgacc
     2821 tcggcgacat gggcgagaaa ctcgaccgcc tcgacgtggt ggaagttgcc gatcgtcgag
     2881 cccgggaagt agaccacccg acgacgcgga gccgaccggg tcgaagggat cggatagtcg
     2941 agcgtgtagt cggcacagac gggcagcacc tcgacgtccg ggaagcgtcg ccgcaggcgc
     3001 gccgcggaag cggagaggaa ctcacacgag atatcgatcg gcacgtaggc gacgggacgg
     3061 tcgagcgcct ccagcagcac ctccgtcttg tgaccggcgc cgctgccgta ctcgagcacc
     3121 aggcagcgcg ggcccagcgc cgccgccatc tcgtccacgg ccgcgtccat gatcgccagc
     3181 tcggtgcgcg tcaggtagta ctcgtcgagc tcg
//
LOCUS       DBNGNK010000165         2443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1532835,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000165 DBNGNK010000000
VERSION     DBNGNK010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1532835"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="AC150V_04780"
     CDS             complement(<1..123)
                     /locus_tag="AC150V_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009741821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762271.1"
                     /translation="MARLDGKVAIVTGAGRGIGRAIVDRLTAEGAAVVASQRTVS"
     gene            180..1118
                     /locus_tag="AC150V_04785"
     CDS             180..1118
                     /locus_tag="AC150V_04785"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3762272.1"
                     /translation="MDGGSTGSDPHNWRGLFPAICTPFMDDGAVDHDAARRVVRFALD
                     QGSHGIVAFGLAGEVLKLTADERRALTDVIVSEVAGAVPVLVGAGAPSVRESIELARY
                     AESAGASGVVLPAPTSGALNDAALVDYFVEVASAVGVPVMIQDAPAYLGVGLGPGIVS
                     QIGARAENVRLVKLEAGPVEMSAWIERLGDDFAIWGGDGGVYMLDCVRAGAAGIIPGV
                     DLIDLLLLAYEAESRGESALAEERFREILPILVFEIQHSIDHFNACAKRVLVERGVLD
                     NPGLREPAESFGDVSSMLLDRHLAGLRFESVGAGVG"
     gene            1102..1887
                     /locus_tag="AC150V_04790"
     CDS             1102..1887
                     /locus_tag="AC150V_04790"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3762273.1"
                     /translation="MPVSADRQPPAEEMVEPRRLSRQVSARLAAEIVSGRIGVGEAFP
                     TSEQIVARFGVSRTVARETVQALAMLGLVKVQHGKRTEVCPPEDWDILSSVVQEAMRR
                     EGKAEPLLRDLYEFRLLIEPQAVGWMADHADPEDVAELDALAAKMEAIAQDGTAGAEV
                     MEADRHFHDHIARASDNRILAAVSRDIREVISTLWGFSALDPSTAQQAAAQHREIADA
                     VESRDAAAAANAMREHLLWAAQADLHGLGEPSQLRLPSGTAVG"
     gene            1916..>2443
                     /locus_tag="AC150V_04795"
     CDS             1916..>2443
                     /locus_tag="AC150V_04795"
                     /inference="COORDINATES: protein motif:HMM:NF017215.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3762274.1"
                     /translation="MGLVIGIDTGGTFTDMVVLDPATGSVAALKTSSIPSTPGKAIAN
                     ALDEGGVTAAEIQTFTNGTTVGTNALIERTGCKVAYVTTKGFEDTPFIQRINRKVLYD
                     LRWRKPEPLVASRRLCVGLDERLDSSGSEIRPVDEGEVRALCHTIRDSDAEAVAVCLL
                     FSYVSTEHEERVKQIF"
BASE COUNT          396 a          803 c          862 g          382 t
ORIGIN      
        1 cgagacggtt ctctgggacg cgactaccgc cgctccctcc gcggtcagtc ggtcgacgat
       61 cgccctgccg atcccgcgcc cggcgccggt tacgatcgct accttgccgt ccagccgcgc
      121 cagtgtaggc cttccacttg tcatcttgga tgataagcta accagtctct gacttccgcg
      181 tggacggagg ctctacgggc tcggatcccc acaactggcg cggcctcttt cccgcgatct
      241 gcacaccgtt catggacgac ggcgcggtcg accacgacgc cgcgcgccgc gtggtgcggt
      301 tcgcactcga ccagggatct cacgggatcg ttgcgttcgg cctggcgggc gaggtcttga
      361 aactcaccgc cgacgagcgc cgtgccctga ccgacgtgat cgtctcggag gttgcgggcg
      421 ccgtacccgt attggtcggc gcaggcgcac cgagtgtccg cgagtcgatc gagctggcgc
      481 gttacgcgga gagcgccggc gcgagcggtg tcgtcctgcc cgcgccgacg agcggcgcgc
      541 tgaacgacgc ggcgctcgtc gactacttcg tcgaggtcgc ttctgcagtc ggcgtgccgg
      601 tgatgattca ggacgcaccc gcgtacctcg gtgtcgggct cgggcccggg atcgtgagtc
      661 agatcggagc gcgggccgag aacgtacgcc tcgtgaagct cgaggcgggc ccggtcgaga
      721 tgagcgcgtg gatcgagcgc ctgggcgacg acttcgcgat ctggggtggg gacggcggcg
      781 tctacatgct cgactgcgtt cgagcaggcg cggcgggaat catcccgggc gtcgacctga
      841 tcgacctcct gctgctggcc tacgaggcgg agtcgcgggg ggagagcgcg ctcgcagaag
      901 agcgattccg cgagatcctg ccgatcctcg tcttcgagat ccagcactcc atcgaccact
      961 tcaacgcctg cgcgaagcgt gtcctcgtcg agcgcggcgt cctggacaat cccggtctgc
     1021 gagaacccgc cgagtcgttc ggcgacgtgt cgagcatgct gctcgatcgg cacctcgccg
     1081 gcctccggtt cgaatccgtc ggtgccggtg tcggctgacc ggcaaccgcc ggccgaggag
     1141 atggtggagc ctcggcgtct cagccgccag gtgtcggcgc ggctcgccgc cgagatcgtg
     1201 tccggccgga tcggcgtggg tgaggcgttc ccgacctcgg agcagatcgt ggcccgcttc
     1261 ggcgtcagcc gcacagtggc tcgcgagacg gtgcaggccc tcgcgatgct cggcttggtg
     1321 aaggtgcagc acggcaagcg aaccgaggtg tgccctcctg aggactggga catcctgagc
     1381 tccgtggtgc aagaggcgat gcggcgcgag ggcaaagccg agccgctgct gcgcgatctc
     1441 tacgagttcc gcctgctgat cgagccgcag gcggtcggat ggatggccga ccacgccgac
     1501 cccgaggacg ttgccgaact cgatgcgctc gcggcaaaga tggaggcgat cgctcaggac
     1561 ggcaccgcgg gggcggaagt gatggaagcc gaccgacact tccacgacca catcgcacgc
     1621 gcgagcgaca atcgcattct cgcagcggtc agccgggaca tccgtgaggt gatcagcacg
     1681 ctgtggggct tctcggcgct ggacccgtcg accgcgcagc aggctgccgc gcagcaccgg
     1741 gagatagcag acgcagtcga gagccgcgac gcagccgcag ccgcgaacgc catgcgtgag
     1801 cacctcctgt gggctgcgca ggcggacctc cacggactcg gcgagccgtc gcagctgcgc
     1861 ctcccctcgg gaacagccgt agggtagagc ggcagttcga cactcgggca cgagattggg
     1921 actcgtcatc ggcatcgata cgggcgggac gttcaccgac atggtcgtcc tcgaccccgc
     1981 aaccggatcg gtcgcggcgc tcaagacgtc gtccatcccg agcaccccgg gaaaggcgat
     2041 cgccaacgcg ctcgacgaag ggggcgtcac ggcggccgag atccagacgt tcaccaacgg
     2101 cacgaccgtg gggacgaatg cgctgatcga gcggacgggg tgcaaggtgg cgtacgtgac
     2161 gacgaagggc ttcgaggaca cgcctttcat ccagcggatc aaccggaaag tgctctacga
     2221 cctgcgctgg cggaaaccgg agccgctcgt cgcaagccga cgtctctgtg tcggcctcga
     2281 cgagaggctg gattcctcgg gctcggagat cagaccggtc gacgagggcg aggtgagagc
     2341 gctctgccac acgatcaggg actcggacgc cgaggcggtc gcggtttgcc tgctcttctc
     2401 gtacgtcagc accgagcacg aggagcgggt caagcagatc ttc
//
LOCUS       DBNGNK010000166         4003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_673059,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000166 DBNGNK010000000
VERSION     DBNGNK010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_673059"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="AC150V_04800"
     CDS             <1..677
                     /locus_tag="AC150V_04800"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3762275.1"
                     /translation="ATVALLDEENGTMAIGHVGDSRAYRVRDGVLEQLTVDHSLVAEL
                     IRSGQLTEEEASVHPHRAVITRALGTEPGVEVDTHTIELESGDLYLLCSDGLTTMVSD
                     DDVLVALDRADGDPERVARLLVEAANAAGGEDNVTVVVFEIVDGIPVPRSVEPAEPPA
                     EPAEVEETREDVSAITDEHPSEPAPPDDSVRRHGAGHGSRILALTLIVVVAVIAALVL
                     YLGLVR"
     gene            712..2019
                     /locus_tag="AC150V_04805"
     CDS             712..2019
                     /locus_tag="AC150V_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR3762276.1"
                     /translation="MRVRNRELFNLVLAGLVAAAAFASVTIAISNVVSFESLVWVGVF
                     AGVYLLAHLVARQLVPLADPTLLPLAAVLTAVGLTVIYRLNPDNASRQAVWVVVGLGA
                     FLVTLVWLRRDYRVLERYKYLFGISAVALLLLPSIPGLGLEVNGVRLWAKLGPLQFQP
                     GELAKILLVVFLAGYLRDKREALAQGRLKDFGPLLAIWGAAMLVLVQTSDLGSALLNF
                     GIFLAMLYAATGRGLYVAVGIGLFVAGAAALYNALDRVQQRVTVWLHPWTDERVACSD
                     GSFDFRQYCDSYQLVKSLYSIANGGYGGTGLGRGTFSSVQGDPLIPFLNTDFIYSAIA
                     QELGLIGSAAVLLAFMVFVARGMRIALVAQDGFSKLLAAGLTFGFALQTFIIVGGVLR
                     VVPLTGITLPFVSYGGSSIVSNFVMLALLLLISNRAAAGAGAR"
     gene            2016..3494
                     /locus_tag="AC150V_04810"
     CDS             2016..3494
                     /locus_tag="AC150V_04810"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR3762277.1"
                     /translation="MNRQITRVAGVAMLLLVGLVVATTYWQTWARPALADRQDNEIQR
                     VAQFSVERGEIRAPARRLARNREVEVDSRPLFLRRYPQGKLTAHVVGYSTASRARAGL
                     ERSLNDYLTGSNKDLSTVVDSSLDKLRGEPVKGSHVEVTLDLKAQRIALDELGTRCGA
                     VAALDPRTGRILVLASSPSYDPNFVENNFSAIERIGAECSPAAPLVNRATAGLYPPGS
                     TFKVVTAAAALDSGEYTPDSTFNDPGYCIAYGKRVNNYDTSRPFGTISLKTALQYSVN
                     SVFCNIGQELGSKRILDEAREFGFYELPPLETPENERRASGLYRNGELYYPERDSDVD
                     AGRTAFGQERLLTTPLQMAMVAGTIGNAGILMRPYVVDRIIAPGGKVLEKTERERIRR
                     AVKPTTARDVSEMMVRAVEAGTGTSAIIPGFRVGGKTGTAETGVAGLNTTWFIAFAGP
                     RDERPTVAVAVALENQSSTGGATAAPIARAVMQALLQPAANT"
     gene            3508..>4003
                     /locus_tag="AC150V_04815"
     CDS             3508..>4003
                     /locus_tag="AC150V_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013625487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3762278.1"
                     /translation="MRASEEITGTLFASRYRLERKLGTGGMADVWLAEDQELGRRVAV
                     KILHERYASDEQFVERFRREATHAAALSHPNIVSIFDRGTADGSYYIVMEYVEGRTLK
                     ELIVTRGPCPVPIASSYTRQILAALRYAHRNGIIHRDVKPHNVIVDREGRVKVMDFGI
                     ARAGT"
BASE COUNT          631 a         1393 c         1311 g          668 t
ORIGIN      
        1 ccgctacggt cgcgctgctc gacgaggaaa acgggacgat ggcgatcggg cacgtcggcg
       61 actcgcgtgc ctaccgcgtt cgcgacggtg ttctggagca gctcaccgtc gaccactcac
      121 tcgtcgccga gctgattcga agcggccagc tgaccgagga agaagcctcc gtccatccgc
      181 atcgggcggt gatcacgcgc gcactgggca cggaacccgg tgtcgaggtc gatacgcaca
      241 cgatcgagct ggagtccggc gacctctacc tgctctgctc cgacgggctc acgacgatgg
      301 tgagcgacga cgacgtcctc gttgcgctcg accgggccga cggcgatccc gagcgcgtcg
      361 cgcgtctgct cgtcgaggcg gccaacgcgg cgggcggcga ggacaacgtg acggtggtcg
      421 tgttcgagat cgtcgacggg ataccggtgc cgagatccgt cgagcccgcc gagccgccag
      481 cggaacccgc ggaggtcgag gagacgaggg aggacgtctc ggcgatcacc gacgagcatc
      541 cgtcggagcc ggcgcctccg gacgattcgg ttcgtcggca cggtgcgggt cacgggagcc
      601 gcatcctcgc gttgacgctg atcgtggtcg tcgcggtgat cgcggccctc gtcctctatc
      661 tcgggctcgt tcgatgaccc atccctcgga cgcttcgagc cgctcgccga gatgagagtt
      721 cgaaaccgcg agctgttcaa cctcgtcctg gccggcctcg tcgcggcggc tgcgttcgcg
      781 agcgtcacga tcgcgatctc caacgtcgtc tcgttcgagt ctctcgtgtg ggtcggcgtg
      841 ttcgcaggcg tctacctcct cgcgcacctc gttgcgcggc agctcgtgcc gctcgccgac
      901 ccgacgctgc tgccgctggc cgcagtgctc accgcggtcg gcctcaccgt gatctaccgg
      961 ctcaacccgg acaacgcgtc acgacaggcg gtgtgggtcg tggtcggcct cggagcgttt
     1021 ctcgtcacgc tcgtctggct ccgccgtgac tacagggtgc tcgagcgcta caagtatctc
     1081 ttcggcatca gcgcagtcgc gctcctgctg ctgccgtcca tcccggggct agggctcgaa
     1141 gtcaacggcg tccgtctctg ggcgaagctc ggcccgctcc agttccaacc gggcgagctc
     1201 gcgaagatcc tgctcgtcgt gttcctcgcc ggctacctgc gggacaagcg cgaagcgctg
     1261 gctcagggcc gcctgaagga cttcggtcct cttctcgcga tctggggtgc tgcgatgctg
     1321 gtgctcgtcc agacaagcga cctcggcagc gcgctcctca acttcgggat cttcctggcg
     1381 atgttgtacg cggcgacggg acggggtctc tacgtcgccg tcggcatcgg cctcttcgtc
     1441 gcgggcgcgg ccgctctgta caacgccctc gaccgcgtgc agcaacgcgt gaccgtgtgg
     1501 ctgcacccct ggaccgacga gcgagtcgcc tgctccgacg gcagcttcga cttccggcag
     1561 tactgcgact cgtaccagct cgtgaagagc ctgtactcga tcgcgaacgg aggctacggc
     1621 ggcaccgggc tcggccgcgg cacgttctcg tccgtccagg gtgacccgct catccccttc
     1681 ctcaacaccg acttcatcta ctcggcgatc gcacaggagc tcggtctgat cggctcggcg
     1741 gccgtgctgc tcgcgttcat ggtgttcgtc gcacggggaa tgcgcatcgc cctcgtcgcc
     1801 caggacggct tctcgaaact gctcgccgcc gggctgacct tcgggttcgc tctgcagacg
     1861 ttcatcatcg tcggcggcgt cctccgcgtg gttccactca ccggaatcac ccttccgttc
     1921 gtctcatatg gaggatccag catcgtttcg aacttcgtca tgctcgcact cctgcttctc
     1981 atctcgaatc gggccgccgc cggggcggga gcgcggtgaa ccgccagatc acccgcgtcg
     2041 ccggcgtcgc catgctcctg ctcgtcgggc tcgtggtcgc gaccacctac tggcagacgt
     2101 gggcgagacc cgcgctcgcg gaccgacagg acaacgagat ccagcgtgtt gcccagttct
     2161 ccgtcgagcg gggcgagatc cgtgcgcccg cgcgccggct ggcacggaac cgggaggtcg
     2221 aggtcgacag ccggccgctc ttcctgcgcc gttacccgca aggcaagctg accgcgcacg
     2281 tcgtcggcta ctcgaccgct tcccgggcgc gggccggact cgagcgatca ctcaacgact
     2341 acttgaccgg ctcgaacaag gatttgtcga cggtggtcga ctcctcgctc gacaagctgc
     2401 gcggcgagcc ggtgaaggga agccatgtcg aagtcaccct cgacctcaag gcacagcgga
     2461 tcgccttgga cgagctcgga acccgctgcg gcgcggtcgc tgcgctcgat cctcgcaccg
     2521 gccggattct cgtgctggcg tccagcccga gctacgaccc caacttcgtc gagaacaact
     2581 tctcggcgat cgagcggatc ggcgccgagt gcagcccagc cgctcctctc gtcaatcgcg
     2641 ccaccgcagg cctctacccg ccgggatcga ccttcaaggt cgtcaccgcg gccgcggcac
     2701 tcgactccgg cgagtacacg ccggattcga ccttcaacga ccccggatac tgcatcgcct
     2761 acggcaagcg ggtgaacaac tacgacacgt cgcggccgtt cgggacgatc tcgttgaaga
     2821 ccgcgctgca gtactccgtc aactccgtct tctgcaacat cgggcaggag ctcgggtcga
     2881 agcgcatcct cgacgaggca cgggagttcg gcttctacga gctcccgccg ctcgagacgc
     2941 cggagaacga gcgccgggcg agcggcctct accggaacgg cgagctctat taccccgagc
     3001 gcgacagcga cgtggacgcc ggccgcactg ccttcgggca ggagcggctg ctgaccacgc
     3061 cgctgcagat ggccatggtg gccggcacga tcggaaacgc tggcatcctg atgaggcctt
     3121 atgtcgtcga ccggatcatc gccccaggcg gcaaggtgct cgagaagacg gagcgcgagc
     3181 ggatccgccg cgcagtcaag ccaaccaccg cccgcgacgt cagcgagatg atggtgcgcg
     3241 ccgtcgaggc cggaacgggt acctctgcga tcatccccgg attccgcgtc ggcggaaaga
     3301 ccggaaccgc cgaaaccggc gtcgcgggtc tcaacacgac ctggttcatc gcgttcgcgg
     3361 gcccgaggga cgagaggccg accgtcgccg tcgccgtcgc gctggagaat cagtcgtcga
     3421 cggggggcgc gacggcggcg ccgattgccc gcgcagtgat gcaggcactc ctacaaccgg
     3481 cggcgaacac ctaacgttac gggtcgaatg agagctagcg aggagatcac cggcaccctg
     3541 ttcgcaagcc gctaccgcct ggagcggaag ctcggcacgg gcgggatggc cgacgtctgg
     3601 ttggcggaag accaggagct cgggcgccga gtcgccgtca agatcctgca cgagcgctac
     3661 gcgagcgacg agcagttcgt cgagcgcttc cggcgggagg cgacccacgc ggctgccctc
     3721 tcgcacccca atatcgtgtc gatcttcgac cgcggcacgg ccgacggctc gtactacatc
     3781 gtcatggagt acgtcgaggg gcgcaccctg aaggagctca tcgtcacccg tgggccgtgc
     3841 cccgtcccga tcgcgtcctc gtacacgcgt cagatactcg cggcgctccg ctacgcgcat
     3901 cgcaacggga tcattcatcg tgacgtcaag ccgcacaacg tgatcgtgga ccgggaaggc
     3961 cgggtcaagg tcatggactt cggtatcgcg cgcgccggga cga
//
LOCUS       DBNGNK010000167         7675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1032374,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000167 DBNGNK010000000
VERSION     DBNGNK010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7675
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1032374"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1459)
                     /locus_tag="AC150V_04820"
     CDS             complement(89..1459)
                     /locus_tag="AC150V_04820"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3762279.1"
                     /translation="MKAAVAAGHPATATAGMEILEDGGTAADAAIGMCLASCVAETVM
                     TGLLGGGHAIWWNGHRAANLDCFVAVPARDGEMVDLPLDFGEEIVEYTVGPGSCAVPG
                     VPAGLDALHRAGGSLPWNRLVEPALRLARSGVAMPPRHIATLSMLEPAMAVGRGATFY
                     APDGRLLGPGDLLQQPGLVDTMECIAEEGPASAYRGSIGEALLAVENVVLDWDDLKGY
                     EARWHRPARAEWHGFHVLTRSGLSGVPELLGRMPRLGGRTEEERVLTLVAALEDVPTP
                     ESETHTTNVVAVDEMGRACVLTTTLGLGATVWTPWFDLHLNSMLGEQDLIVGDLVPGE
                     RMESMMAPTIAFDAEGLSLTVGSAGGTRLRTALVTVLAGVLDEGLAPQDAIDRPRVHA
                     TNDAVHAEPGVDEKALARLETQHRKVRRWPELHHYFGGASCVGREGAAGDPRRNGLGI
                     VLGETG"
     gene            1615..1884
                     /gene="rpsO"
                     /locus_tag="AC150V_04825"
     CDS             1615..1884
                     /gene="rpsO"
                     /locus_tag="AC150V_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR3762280.1"
                     /translation="MALTKEVKQELITKHGRTQADTGSPEVQIAMLTQRINDLTEHLR
                     THPKDHYSRRGLLKLVGRRRRFLDYLQRKNLEGYRALIKELGLRR"
     gene            1995..4478
                     /locus_tag="AC150V_04830"
     CDS             1995..4478
                     /locus_tag="AC150V_04830"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR3762281.1"
                     /translation="MSTTAVHDATVSVEIGGNEITFETGKLAKQASGAVAVRSGDTMI
                     LATAVGRPDTRPDADFFPLTVDVEERMYAAGKIPGGFFKREGRATERATLTARMIDRP
                     IRPLWPKGFRNEVHIVCTVLSADLITPHDTLCINGASAALHVSPLPFLGPVGAVRIGR
                     LDGALVVNPSLPQLEDEASSLDLIVVGTREALTMIEAGADEVPEETILDAFALAHQEI
                     LRICDALDELRAQVGKPKWIDLELNAELEREHAGKISAAIGRHGLREAATVVDEIVAQ
                     SSPPITMESTESDIVRETQIRNGLQALLEKERLVAVEGPVRAQFEAELRLLTDAEQDS
                     KELKSRKRDILYTRIVEDAQLPFPVGSAPEGETAVADGMTRQYVRRAAEGIYKDLVRR
                     KIAVEKRRPDGRGTEEVRPVDCDVTVSPRTHGSALFTRGQTQIMTLCTLGTAKEGQRI
                     DDLSLEAERRYMHHYNFPPYSVGETGFMRGPKRRDIGHGALAQRALEPVIPATDEFPY
                     TIRLVSETLESNGSSSMGSVCGSTLALMDAGAPIKAPVSGIAMGLVKEGDDYVILTDI
                     QGAEDHLGDMDFKVAGSPNGITALQMDITITGVTQEIMRSALDQARRAREFILERMLE
                     AISEPRTELAGHAPRISTIKINPEYIGMVIGKGGETIRALESEFEVQIDIEEDGTILI
                     YATEGTKADAAIAEIDALTKDPEVGDTYTGKVVKTTQFGAFVELKKGTDGLLHVSNVG
                     PGRVGHIEDVIVRGDVLDVVVQEVDRDRGRIGLKLVAKHENGSLVQPEELIERAKNAP
                     PREERDERPRGDRRGGRDRGRGGRGRDRP"
     gene            4494..5825
                     /locus_tag="AC150V_04835"
     CDS             4494..5825
                     /locus_tag="AC150V_04835"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR3762282.1"
                     /translation="MGTLVVPTSGAVTITRMSGHELTALPGGVRVISEQIDTVRSVSL
                     GFWIGSGSRDETNVRAGVSHFIEHLLFKGSDRYTAVEIAEIFDAMGGELNAATSRETT
                     VVYARVPDDSLERALDVMADMVFRPSFADVDQEREVVLEEIAMVDDNPQDHVHDLAAE
                     ALFASHPLGRPVIGSADVIRSVGRRTLRAHHRAAYAGRNVVVAAAGNVDHGRLAELVA
                     ARVPEGSVQGRLPRKQLRRRPSPGLRFLPRDTEQYHVCLSGSGIARDDERRFAGALLD
                     AIVGGSSSSRLFQEIREKRGMAYSVYSYASQYADSGQVGLYVGTREENLAECLDVVAH
                     ELHDLGAGNIRPGELERAKENIKSRLLLSLESTSSRMTRLGKALVTDVELLDVEEVLE
                     RVITVEPGEVATLAAELFAPELLSAAAIGPRERSFRDAVEHVNPVLLAKAA"
     gene            5835..6479
                     /locus_tag="AC150V_04840"
     CDS             5835..6479
                     /locus_tag="AC150V_04840"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011725599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016726 - oxidoreductase activity, acting
                     on CH or CH2 groups, NAD or NADP as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR3762283.1"
                     /translation="MRVVLFGRDGKVGSVLGPALREAGHDVTGIEIDEEAAVEGYDAA
                     VDFTAPDSVLGNATDCLEAGVPCLIGTTGLTDEGLASLDRLARDRGIPCFVAPNFAIG
                     AVLMMRFAEQAAGAMPHAEIIELHHETKVDAPSGTAKATAERMGGDVPIHSVRLPGLV
                     AHQEVILGAPGQTLSIRHDTTSRDAFVPGVLLALEKLGSLPPGLTVGLDAVLDG"
     gene            6472..6981
                     /locus_tag="AC150V_04845"
     CDS             6472..6981
                     /locus_tag="AC150V_04845"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR3762284.1"
                     /translation="MAEIAFVVGDLTAQRVDAIVNAANESLAPGGGVCGAIHRAGGPR
                     IADECARLGGCATGDAKATGGGDLPASWVIHAVGPVWHGGEQGEPELLASAYRRSIEV
                     AAGLGAQTVAFPAISCGIYGYPPELAAPTAVGAVREALAANPAVDEARFVFLDDDLQA
                     VFAQAAESR"
     gene            complement(6999..>7675)
                     /locus_tag="AC150V_04850"
     CDS             complement(6999..>7675)
                     /locus_tag="AC150V_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3762285.1"
                     /translation="RSGDLAVSIEQGRCVADAIPGARFVELPGDDHHVWIGDQDAVLD
                     EVERFLSELGPAPAVERALATVLFTDIVASTETASRLGDRRWRDVLEEHHRLVREQLD
                     KCGGEEVRTVGDGFLATFSAPAGGIRCAQAISAAVQPLGIAVRVGVHTGECELIDGEV
                     GGIAVVIGARITELAGPSEVLVSQTVKDLVAGSGLEFEDRGVYTLKGVPDEWRLYAAQ
                     ANATAG"
BASE COUNT         1238 a         2650 c         2659 g         1128 t
ORIGIN      
        1 gcctcggccg cgtactcggc gggactcgtc agcaggtgga accacggcgc caggtcgctg
       61 tagagcgcgg gcgtgccagc gtcggcggtc acccggtctc tcccaagacg atgccaagcc
      121 cgttgcgccg cggatcgccg gccgcgccct cgcggccgac gcagctggct ccgccgaagt
      181 aatgatggag ctcgggccag cgccgcacct ttcggtgctg ggtctcgagt cgtgcgagcg
      241 ccttctcgtc gacaccgggc tctgcgtgca cggcgtcgtt cgtcgcatgc actctcggcc
      301 ggtcgatcgc gtcctgcgga gcgagcccct cgtcgaggac gccggccaga acggtgacca
      361 gcgcagtccg caggcgcgtg ccgcccgcgg agccgaccgt cagcgaaaga ccttctgcgt
      421 cgaaggcgat ggtcggcgcc atcatcgact ccattcgctc gccgggtacg aggtcgccca
      481 cgatgaggtc ctgttcgccg agcatcgagt tgaggtgcag gtcgaaccag ggtgtccaga
      541 ccgtcgctcc gaggccgagc gtcgtcgtga gcacgcaggc gcggcccatc tcgtcgacgg
      601 cgacgacgtt cgtcgtgtgc gtctcgctct cgggtgtggg cacgtcctcg agtgcagcga
      661 cgagggtgag cacgcgctcc tcctcggtgc gcccgcccag tcgcggcatg cgtccgagca
      721 gctcgggtac gcccgacagc cccgatcgcg tcagcacgtg gaatccgtgc cattcggctc
      781 gcgcagggcg gtgccagcgc gcctcgtagc ccttcaggtc gtcccagtcc agcacgacgt
      841 tctcgacggc gaggagcgcc tccccgatcg aaccgcgata cgcactggcc gggccctcct
      901 ccgcgatgca ctccatggtg tcgaccagcc ccggctgctg aagcagatcg ccgggcccga
      961 gcagccgacc gtccggtgca tagaacgtgg cgccgcggcc tactgccatc gcgggttcga
     1021 gcatcgagag cgtggcgatg tgccgcggcg gcatggcgac cccgctgcgc gccagccgga
     1081 gcgcgggctc gacgaggcgg ttccagggga gcgagccgcc ggcgcggtgg agcgcatcga
     1141 gaccggcagg cactcccggc acggcgcacg agcccgggcc gaccgtgtac tcgacgattt
     1201 cttcgccgaa gtcgagcggc aggtcgacca tctcgccgtc ccgggccggc acggcgacga
     1261 agcagtcgag gttggcagcg cggtgaccgt tccaccagat cgcatgaccg ccgccgagga
     1321 ggcccgtcat gacggtctcg gcaacgcacg acgccaggca catcccaatc gcggcgtcgg
     1381 ccgcagtgcc accgtcttcc aggatctcca tgccggcggt cgcggtcgct gggtgccctg
     1441 cggcgacagc agccttcatg gcggcggacg ctagcgcacc gcgaaccgcg ccgacccgcg
     1501 aacgggaggc gccagcgcgg ctgctagcct tcctgcgacc gcagtcgcgg ccggccgtgt
     1561 tcttccttca cgccgcgcgg ggactgcgga gacgcatcca cagagaggag aacgatggcc
     1621 ctcaccaagg aagtcaagca ggagctgatc acgaagcacg gccgcaccca ggccgacacg
     1681 ggctcgcccg aggtacagat cgcgatgctg acgcagcgca tcaacgatct gaccgagcat
     1741 ctccgcacgc acccgaagga tcactactcg cggcgcggac tgctcaagct cgtcggccgg
     1801 cgccgccgct tcctcgacta cctgcaacgc aagaacctcg agggctatcg ggccctcatc
     1861 aaggagctcg gcctccgccg atagctctgg aagcgggagg tggacgtttg atcgagctcc
     1921 gagccgttcg cagacgcggc ggagctggct caaacctcca ccttccggca atggaagagt
     1981 gaagggatag acagatgagc accacagccg tgcacgatgc aacggtttcc gtcgagatcg
     2041 gtggcaacga gatcaccttc gagacgggca agctcgcaaa gcaggcaagc ggggcagtcg
     2101 ccgtccgaag cggcgacacg atgatcctcg cgacggcggt cggccgtccg gacacccgcc
     2161 cggacgcgga cttctttccg ctgaccgtcg acgtcgagga gcgcatgtac gccgccggga
     2221 agatccccgg cgggttcttc aagcgggagg ggcgcgcgac ggagcgggcg actcttacgg
     2281 cgcggatgat cgaccgcccg atcaggccgc tctggccgaa gggcttccgc aacgaggtgc
     2341 acatcgtctg caccgttctc tcggcggacc tgatcacgcc gcacgacacg ctctgcatca
     2401 acggtgcctc ggcggccctg cacgtctcgc cactgccgtt tctcggaccg gtcggcgcgg
     2461 tcagaatcgg ccgcctcgac ggggcgctgg tcgtcaaccc atcgctgccg cagctggagg
     2521 acgaggcgtc gtcgctcgac ttgatcgtcg tcgggacgcg ggaagctctc acgatgatcg
     2581 aggccggagc cgacgaggtg cccgaggaga cgatcctcga cgcgttcgcc ctggcccacc
     2641 aggagatcct gcggatctgt gacgcgctcg acgagctgcg cgcccaggtt ggcaagccga
     2701 agtggatcga tctcgagctc aacgcggagc tcgagcggga gcacgcaggc aagatcagcg
     2761 ccgcgatcgg ccggcacggg ctccgcgaag ccgccacggt ggtggacgag atcgtggcac
     2821 agagctcgcc gccgatcacc atggagtcga ccgagtcgga catcgttcgc gagacccaga
     2881 tccggaacgg cctgcaggcc ctcctggaga aggagcgcct tgtcgcggtc gaaggcccgg
     2941 ttcgcgctca gttcgaggcc gagctacgcc tgctgaccga cgccgagcag gactcgaagg
     3001 agctcaagtc ccgcaagcgc gacatcctct acacgcgcat cgtcgaagac gcgcagctgc
     3061 ctttcccggt cggctcggcg cccgagggtg agaccgcggt tgccgacggg atgacccgcc
     3121 agtacgtgag gcgggcggcg gaagggatct acaaggacct cgtccgccgg aagatcgccg
     3181 tcgagaagcg tcggcccgac ggtcgcggca ccgaggaagt ccggcccgtg gactgcgacg
     3241 tgaccgtgtc gccccggacg cacggctcag ccttgttcac ccgtgggcag acgcagatca
     3301 tgacgctctg cacgctcggc acagccaagg aaggccaacg gatcgacgac ctgtcgctcg
     3361 aggcagagcg gcggtatatg caccactaca acttcccgcc ctactcggtc ggggagaccg
     3421 gcttcatgcg cggcccgaag cggcgcgaca tcggccacgg tgccctcgcc cagagggccc
     3481 tcgagccggt gatccccgcg accgacgagt tcccgtacac gatccgcctc gtctccgaga
     3541 cgctggagtc caacggctcg tcgtcgatgg gatcggtctg tggctcgacc ctcgccctga
     3601 tggatgccgg cgcaccgatc aaggcgccgg tctccgggat agccatgggt ctggtcaaag
     3661 agggtgacga ctacgtcatc ctcaccgaca tccagggcgc ggaagaccat ctcggcgaca
     3721 tggacttcaa ggtcgccggg tcgcccaacg gcatcacggc cctgcagatg gacatcacga
     3781 tcaccggcgt cacccaggag atcatgcgca gcgcgctcga ccaggcacgc cgggcccgcg
     3841 agttcatcct ggagcggatg ctcgaggcga tctccgagcc gcgcaccgag ctcgcgggac
     3901 atgcaccgcg gatctcgacg atcaagatca accccgagta catcggcatg gtgatcggca
     3961 agggcggcga gacgatccgc gcgctcgagt ccgagttcga ggtgcagatc gacatcgaag
     4021 aggatggcac gatcctcatc tacgcgaccg aaggcaccaa ggcggacgcg gcgatcgccg
     4081 agatcgacgc gctgaccaag gatcccgagg tcggcgacac ctacacgggc aaggtcgtga
     4141 aaacgaccca gttcggcgcg ttcgtcgagc tgaagaaggg cacggatggc ctgctccacg
     4201 tctcgaacgt cggccccggc cgcgtcgggc acatcgagga cgtgatcgtc cgcggtgacg
     4261 tgctcgacgt cgtcgtgcag gaggtcgacc gggatcgtgg ccggatcggc ctcaagctgg
     4321 tcgcgaagca cgagaacggc tccctcgtgc agcccgagga gctgatcgag cgcgccaaga
     4381 acgccccgcc ccgggaggag cgggacgagc ggccccgcgg agaccgtcgc ggcggccgcg
     4441 accgcggccg gggtggtcgg ggccgcgacc gcccgtagag tgaggcatcg tcggtgggga
     4501 cgctcgtcgt ccccaccagc ggcgccgtta cgatcacgcg catgagcggg cacgagctga
     4561 cagcgctgcc cggcggcgtt cgcgttatct cggagcagat cgacacggtc cgctccgtct
     4621 cgctcggctt ctggatcggg tcggggtctc gcgacgagac gaacgtgcgt gccggggtgt
     4681 cccacttcat cgagcacctg ctcttcaagg ggtcggaccg ctacacggcc gtcgagatag
     4741 cggagatctt cgacgccatg ggcggtgagc tcaacgcggc gacctcgcgc gagacgaccg
     4801 tcgtctacgc gcgtgtgccg gacgacagcc tcgagcgggc gctcgacgtc atggccgaca
     4861 tggtcttccg gccgtcgttc gcggacgtcg atcaggagcg cgaagtcgtg ctcgaagaga
     4921 tcgcgatggt cgacgacaac ccgcaggatc acgtccacga cctcgcggcc gaggctttgt
     4981 tcgcgtcgca tccactgggt cgtcccgtga tcggcagcgc ggacgtgatc cggtccgtcg
     5041 ggcgccggac gcttcgcgcc caccatcgcg ccgcgtacgc cggtcgcaac gttgtcgtcg
     5101 cggctgccgg caacgtcgat cacggccgcc tcgccgagct ggtcgcggcg cgcgtgccgg
     5161 aggggtccgt gcaaggccgc ttgccccgga agcagctgcg gcgccgtcca tccccgggac
     5221 tccgcttcct tccccgtgac acggagcagt atcacgtctg cctttcgggc tccgggatcg
     5281 cacgggacga cgagcggcgt ttcgccggcg cgctcctcga cgcgatcgtg ggcggctcgt
     5341 cgtcgtcccg gctcttccag gaaatccggg agaaacgggg catggcgtac tccgtctact
     5401 cgtacgcgtc gcagtacgcg gactcgggtc aggtaggcct ctacgtcggc actcgcgagg
     5461 agaacctggc ggagtgcctg gacgttgtcg cacacgagct gcacgatctc ggcgctggga
     5521 acatccgccc gggagagctc gagcgggcga aggagaacat taagagccgc ctcctgctgt
     5581 cgctggaatc gacctcgagc cggatgacga ggctcggcaa ggcgcttgtc accgacgtcg
     5641 agctgctcga cgtcgaggag gttctcgagc gcgtgatcac cgtcgagccc ggtgaggtgg
     5701 cgacgctggc cgcagagctg ttcgctcccg agctgctctc tgcggcagcg atcggaccgc
     5761 gtgaacgcag ctttcgcgac gccgtcgagc acgtgaaccc cgtcctgctc gccaaggccg
     5821 catagggatc agggatgcgc gtcgttctat tcgggcgcga cgggaaggtc ggctccgtgc
     5881 tcggcccggc tcttcgcgag gcgggccacg acgtgacagg gatcgagatc gacgaggagg
     5941 cagccgtcga gggctatgac gctgccgtcg acttcaccgc tccggactcg gtgctcggca
     6001 acgcgacgga ctgcctcgag gctggcgtgc cctgcctgat cggcacgacc gggctcaccg
     6061 acgaggggct cgcgtcactc gatcggctcg cccgcgaccg gggcattccg tgcttcgtgg
     6121 cacccaactt cgcgatcggg gccgtcctga tgatgcggtt cgccgagcag gcagcgggcg
     6181 cgatgccgca cgccgagatc atcgagcttc accacgagac gaaggtcgat gcaccctccg
     6241 ggacggccaa ggcgacggcc gagcgcatgg gcggcgacgt tccgatccat tcggtccggc
     6301 tgcccggcct cgtggcccat caggaggtca ttctgggcgc accagggcag acgctctcga
     6361 tcaggcacga cacgacctcg cgcgacgcgt tcgttcccgg agtgctgctg gcgctggaga
     6421 agctcggctc cctgccgccg ggcctcactg tcggactcga cgcagtgctc gatggctgag
     6481 atcgcgttcg tcgtcggcga tctcacggcg cagcgcgtcg acgcgatcgt caacgcggcg
     6541 aacgaatcgc tcgcaccagg tggcggcgta tgcggtgcga tccaccgggc gggtggcccc
     6601 aggattgccg acgagtgcgc gcggctcggc ggctgcgcca ccggtgacgc gaaggcaacc
     6661 ggcggcggcg acctgccggc gagttgggtg atccacgcgg tcggaccggt ctggcacggc
     6721 ggcgagcagg gcgagcccga gctgctcgcc tccgcgtatc gcaggtcgat cgaggtcgca
     6781 gccgggctcg gcgcgcagac cgtcgccttt ccggcgatct cgtgtgggat ctacggctac
     6841 ccgccggagc tggcggcgcc gaccgcggtc ggcgccgtcc gcgaggctct agcggcgaat
     6901 ccggccgtcg acgaggcccg cttcgtcttc ctcgacgacg atctccaggc cgtcttcgcc
     6961 caggcggcag agagcaggta gcacatcgtg ttccttgctc agccggccgt ggcgtttgcc
     7021 tgcgcggcgt agagccgcca ctcgtccggg acgcctttga gcgtgtagac gcctctgtcc
     7081 tcgaactcca ggcccgagcc ggccacgaga tccttcaccg tttgtgagac gagcacctcc
     7141 gaggggccgg ctagctcggt gatgcgcgcc ccgatgacga cggcgatgcc gcccacctcg
     7201 ccgtcgatca gctcgcactc gccggtgtgg acgcccacgc ggacggcgat cccgaggggc
     7261 tggacggcag cggagatcgc ctgcgcgcag cggataccgc ctgcgggagc cgagaaggtc
     7321 gccaggaagc cgtctccgac ggtcctcacc tcttcgccgc cgcacttgtc gagctgctca
     7381 cggacgagac gatggtgctc ttcgagcacg tcgcgccagc gtcgatcgcc tagccgcgac
     7441 gccgtctcgg tggaggccac gatgtcggtg aacagcaccg tcgcgagcgc ccgctccacc
     7501 gcaggtgcgg gaccgagctc ggagaggaag cgctcgacct cgtccagaac cgcgtcctgg
     7561 tcgccgatcc agacgtggtg gtcgtccccg ggcagctcca cgaagcgagc cccgggtatc
     7621 gcgtctgcga cgcaccgtcc ttgctcgatc gacacggcca ggtcgccgga acggt
//
LOCUS       DBNGNK010000168         3707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_387026,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000168 DBNGNK010000000
VERSION     DBNGNK010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_387026"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="AC150V_04855"
     CDS             complement(<1..339)
                     /locus_tag="AC150V_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762286.1"
                     /translation="MSLNDWILSFHLLSAFAMVGAITIFSITMVAMRSADSPGRVLAY
                     ESTNKIANIAVFAGVAGTIVFGVWLAISLDAYQIWDGWIIASIVLWAIAVESGRRVEV
                     AYQPASRRAAE"
     gene            complement(391..861)
                     /locus_tag="AC150V_04860"
     CDS             complement(391..861)
                     /locus_tag="AC150V_04860"
                     /inference="COORDINATES: protein motif:HMM:NF021538.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HIR3762287.1"
                     /translation="MRYEWALFLHLASAFAFVGGSVAAAVLRLAALRPSQPGHVALLL
                     RAVRPVVPLVAGGLVLTVAFGFWLVDIVNADLGATWLTLTFVLVAWLLAVGTVTGRQD
                     RHTRELAERLAEESDSPSEELSRRLRDPLNLTLSLTLLLAAAAVVALLVWRPGA"
     gene            901..1374
                     /locus_tag="AC150V_04865"
     CDS             901..1374
                     /locus_tag="AC150V_04865"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HIR3762288.1"
                     /translation="MLTFFRVLHILSASVWVGGTVALVFIAVPAVRKLDGAARATAMR
                     TLGRRWRPIGWSAMGVAILSGLWLSDDHGAFSSAALSTEFDRWLIAKSAVVAVLCVGA
                     VLHDYVLGPRLQRQLGRQSPDAPVTRRRLIAVGWFNFALTIAVPVLGVIILGLVD"
     gene            1374..2774
                     /locus_tag="AC150V_04870"
     CDS             1374..2774
                     /locus_tag="AC150V_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3762289.1"
                     /translation="MDETALHLRLLTRTIDVVNSSLDLQEVLEAVAHEVAAALETDAC
                     FVYLFDESAGQLVLRATHGTAVEDATRAPRMRLGEGITGTAAADRAPVMIASRAHLDP
                     RFKQFPNLREEEFESILAVPILASDKLEGALNVRTLVPRRFTDGEVELLIAIASQVGQ
                     SIEHAKLFEAARRRVVELEALARISEAVSESLYLEESLEAIVKTTRESLHASGAALVL
                     DGQVAWPEGRAGRFAVRTPLRWKRREIGELVCDRDTPFGDDDRALLEAIAQNAAVALE
                     SGRMALRGVIAQEIHHRVKNNLQTVASLLRLQANAEGVDPRKALGDSVNRILAIAAVH
                     EELTEHREDVVDLAELLERLRSMLVQGLVEGTDVTSRLEPVELAGQRATALALVFSEL
                     LGNALEHGGSHVTIDLSSGDDEIVLEIVDDGDGMNAAADGTGLSIVRALVRDELAGTI
                     ELRQEDGFRAQVVFPR"
     gene            complement(2771..3304)
                     /locus_tag="AC150V_04875"
     CDS             complement(2771..3304)
                     /locus_tag="AC150V_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3762290.1"
                     /translation="MSTLPRLFHHRWALPIMIDLHANGGAKFVTIVKRLGIGRDSLRR
                     TLGALQSAGLVRRNPGHGHPLRPEYVLTEPGRALAAPAEQLVAALRSQSLEEIGLRKW
                     SVPVLLGLWSGDDHFGAIRARYDISPRALTLALKDLVAADLVERRVHEGFPPTTSYRL
                     TPPGRALAAQAARLEVD"
     gene            3370..>3707
                     /locus_tag="AC150V_04880"
     CDS             3370..>3707
                     /locus_tag="AC150V_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3762291.1"
                     /translation="MDLRFGSNVVCALGVTDLERSLAWYTETLGFEPVYVLEEMGWAE
                     LRTHVTNVVVGLSQVEEVTRGGGATMTFGTSDLDAARAELEQAGVVFDGETIVIPGQV
                     KLATVYDPDG"
BASE COUNT          607 a         1205 c         1324 g          571 t
ORIGIN      
        1 ctcggcagcc cgccggctcg ccggctggta cgcgacctcg acacgccggc cgctctcgac
       61 ggcgatcgcc cacaggacga tcgaggcgat gatccagccg tcccagatct gatacgcgtc
      121 gagcgagatc gcaagccaca cgccgaagac gatcgtgccg gcgacgcccg cgaagaccgc
      181 gatgttcgcg atcttgttgg tcgactcgta ggcgagaacg cggcccgggc tgtcggccga
      241 gcgcatggcg accatcgtga tcgagaagat ggttatcgct ccgaccattg cgaacgccga
      301 aagcaggtgg aaggagagaa tccagtcgtt caggctcatc ggatcggcgg agcctacgcc
      361 agcgactggt tgccgggcaa ggggccgcgg tcaggcgccc gggcgccaga ccaacaacgc
      421 gaccaccgct gcggcggcga ggagcagcgt gaggctgaga gtcaggttga gcgggtcgcg
      481 cagtcgtcgc gagagctcct cggacgggga gtccgattcc tccgccagcc gttccgccag
      541 ctcgcgggtg tgccgatcct gacggccggt gaccgtcccg accgcgagca gccaggcgac
      601 gagcacgaac gtcaacgtca accaggtcgc cccgaggtcc gcgttcacga tgtcgacgag
      661 ccagaagccg aaggcgaccg tgagaacgag tccgcctgct acgagcggca cgacgggacg
      721 gacggcccgg aggagcaggg cgacgtgacc cggctgcgag gggcgcagtg cggcgaggcg
      781 gagcacggcc gcggcgacgc tcccgccgac gaacgcgaac gcggacgcga gatggaggaa
      841 gagcgcccac tcgtaacgca tcgtggagag actacaagcg tcatctagcc tggaacgcag
      901 atgctgacct tcttccgtgt gctccatatc ctctccgcct cggtctgggt cgggggaaca
      961 gtcgcgctcg tgttcatcgc cgtcccggct gtacgcaagc tcgacggggc tgcgcgcgcc
     1021 acagcaatgc ggacgctcgg ccgccgctgg cgtcccatcg gctggagtgc catgggggtc
     1081 gcgatcctgt ccggcctctg gctctcggac gatcacggcg cgttcagcag cgcggcgttg
     1141 agcaccgagt tcgaccgctg gctgatcgcc aagtccgcgg tcgtcgccgt cctctgtgtc
     1201 ggggccgtgc tgcacgacta cgtcctcggg ccgcgactgc aacgccagct cggccggcag
     1261 tcgccggacg cacccgtcac gcggcgtcgc ctgatcgccg tcggctggtt caacttcgcg
     1321 ctgacgatcg ccgttcccgt gctgggcgtg atcatcctcg gcctggtcga ctgatggacg
     1381 agaccgctct ccacctgcgc ttgctgacgc gcacgatcga cgtcgtcaac tcgagtctcg
     1441 acctgcagga agtgctcgag gcggtcgccc acgaggtcgc cgccgcgctc gagacggacg
     1501 cgtgcttcgt ctacctcttc gacgagtcgg ctggtcagct cgtgttgcgc gcgacacacg
     1561 gcacggcggt cgaagacgcg acccgcgctc cccgcatgcg tctgggcgag gggatcaccg
     1621 gcacggccgc cgccgaccgg gctccggtga tgatcgcttc gcgggcccac ctcgacccgc
     1681 gcttcaagca gttccccaat cttcgcgagg aggagttcga gtcgattctc gccgtgccga
     1741 ttctcgccag cgacaagctc gaaggcgcgc tcaacgtccg cacgctcgtt ccgcgcaggt
     1801 tcacggacgg ggaggtcgag ctgctcatcg caatcgcgtc gcaggtcggc cagtcgatcg
     1861 agcacgccaa gctcttcgag gccgcgagga ggcgcgtcgt cgagctggag gcgctggcgc
     1921 ggatctcgga ggccgtctcc gagtcgctct atctcgaaga gtccctcgag gcgatcgtca
     1981 agaccacgag ggaatcgctg catgcgagcg gcgctgcgct cgtcctcgac gggcaggtcg
     2041 cgtggcccga agggcgtgca ggacggttcg ccgtgcggac accgctacgt tggaagcgac
     2101 gggagatcgg cgagctggtc tgcgaccgcg acacgccgtt cggggacgac gaccgcgcgc
     2161 tcctcgaggc gatagcccag aacgccgctg tcgccctcga gagcgggcgg atggccctcc
     2221 gcggtgtgat cgcccaggag atccaccacc gcgtcaagaa caacctgcag accgtggcct
     2281 cgctgctcag gctgcaggcg aacgcggaag gcgtcgaccc gcgtaaggcg ctgggcgact
     2341 ccgtgaatcg catcctggcg atcgccgccg tgcacgagga gctgacagag catcgcgagg
     2401 acgtcgtcga cctggccgag ctgctcgagc gcctccgctc gatgctcgtg cagggcctcg
     2461 tcgagggaac ggacgtgacc tcgcggctcg agccggtcga gctcgcggga cagcgcgcca
     2521 cggcgttggc gctcgttttc agcgagctgc tcgggaacgc gctcgagcac ggcggctcgc
     2581 atgtcacgat cgatctgtcg tccggtgatg acgagatcgt gctggagatc gtcgacgacg
     2641 gagatggaat gaacgcggcg gcggacggaa ctggtctgtc gatcgtccgt gcgctggtgc
     2701 gggacgagct ggccggcacg atcgagctcc ggcaggagga cggatttcgc gcgcaggtcg
     2761 tctttccccg ctaatcgact tcgagccgtg cggcctgggc cgcgagtgct ctaccgggcg
     2821 gagtgaggcg gtacgacgtt gtcggcggga agccctcgtg cactcggcgc tcgaccaggt
     2881 cggcggcgac gagatccttg agcgccagcg tcagcgcgcg gggcgagatg tcgtagcggg
     2941 cccgaattgc gccgaagtgg tcgtcgccgc tccacaggcc gagcaggacg ggcaccgacc
     3001 acttccggag cccgatctcc tcgaggctct gactgcggag cgccgcgacg agctgctccg
     3061 ccggtgccgc gagtgcccgg ccgggctcgg tgaggacgta ctcgggtcgg agcggatggc
     3121 cgtggcccgg gttgcgacgc acgagtccgg cggactggag cgcgccgagc gttcggcgaa
     3181 gcgaatctcg cccgatcccc aggcgtttga cgatcgtgac gaacttcgcg ccgccgtttg
     3241 cgtgcaggtc gatcatgatg ggaagcgccc agcggtggtg gaacagtcga ggcagcgttg
     3301 acacgtaact agattaagta taaaagtaag tacaagggag tctctcgttc ggcaagggag
     3361 gaagaagcga tggatctgcg cttcggctcg aacgtggtct gcgctctcgg cgtgacggat
     3421 ctcgagcggt cgctggcctg gtacaccgag accctgggct tcgagcccgt ctacgtgctc
     3481 gaggagatgg ggtgggcgga attgcgcacg catgtcacca acgtggtcgt gggcctttcc
     3541 caggtcgagg aggtgacccg agggggaggg gcgaccatga ccttcggcac ttcggacctg
     3601 gacgctgcac gcgccgagct cgagcaggcg ggcgtggtgt tcgacggtga gacgatcgtg
     3661 attcccggcc aggtgaagct cgccaccgtc tatgacccgg acggcaa
//
LOCUS       DBNGNK010000169         1838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_745019,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000169 DBNGNK010000000
VERSION     DBNGNK010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_745019"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..298
                     /locus_tag="AC150V_04885"
     tRNA            215..298
                     /locus_tag="AC150V_04885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Leu,seq:cag)
     gene            complement(334..1317)
                     /locus_tag="AC150V_04890"
     CDS             complement(334..1317)
                     /locus_tag="AC150V_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HalD/BesD family halogenase"
                     /protein_id="HIR3762292.1"
                     /translation="MSALPAGPPGYERLDGDTPWDPSRHRKLVRPEKITLLDEWGEGA
                     AGASALSPLAITSPFRFLSDEGVETLQRICAELEQYAVGTRRIPKKMRGSVYRSEFLY
                     GMYHDPEILAFLSELAGTSLEPHPISHHAVHINYAPDDLALNVDQWHRDATAFDYVLM
                     VNDPAPMKGGRFEFFTGPVEEGRDLLESGRGLPSDRVASPEFPGAGWAVLQQGHRVLH
                     RACKLEERYPRITIVASFWAPVPGLDDPTDLPTLITVDGREIALVEWARYQALVAVRR
                     LEHFATTQTDFVHEHEELQAEIRAIAERLLAAADAFESAEEAELISYGAGN"
     gene            complement(1314..>1838)
                     /locus_tag="AC150V_04895"
     CDS             complement(1314..>1838)
                     /locus_tag="AC150V_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3762293.1"
                     /translation="SVAAELLTRHDETVCLAAIDGDDSVLVAVEETSEDVRFVARPGK
                     RTPAFASASGRVILASRRPENLVGDYGGRPLVTPTGRRLAGLAELREILAEVRERGYA
                     VNDEETAAGLWAVAVPITNGQGVVLAALTICVPTSRSHAERRPQLIADLVAAGGRLSD
                     SVAWLPAFTAMTSR"
BASE COUNT          285 a          601 c          662 g          290 t
ORIGIN      
        1 gggggagagg cgaagctcac gacgatctgc gtccgatcgg cgggcgctgc ctgtgttccg
       61 gagccggccg ccggtacgag cacgaggagc agcgctgcgg caagcagcgc caggaggtgt
      121 tggcgcgaag ccgtcgcgag cattcgggtc acatcgtttg agacgagatc gggacgaaaa
      181 cgctggtgca cgctccatct acaatgcctg cgtcgcggat gtggcggaat tggtagacgc
      241 gcacggttca ggtccgtgtg cccgtaaggg cgtggaggtt caagtcctct catccgcatc
      301 ggcgcgatgc ccgcgcgtct cgcgggcgcg tcgctagttg ccggcgccgt acgagatcag
      361 ctcggcctcc tcggccgact cgaaggcgtc ggcggccgcc aaaaggcgct cggcgatggc
      421 gcggatctct gcctggagct cctcgtgctc gtggacgaaa tccgtctgcg tggtcgcgaa
      481 gtgctcgagc cgccgtacgg cgacgagagc ctggtatcga gcccactcga cgagcgcgat
      541 ctcgcggccg tcgaccgtga tcagcgtcgg caggtcggtc gggtcgtcga ggccgggcac
      601 cggcgcccag aaggacgcga cgatcgtgat tcgcggatac cgctcttcga gcttgcacgc
      661 gcgatgcaag acgcggtggc cctgctggag cacagcccag cccgcccccg ggaattccgg
      721 gctcgcgacg cggtcagacg gcagtccccg gccggactcg agcaagtcgc gtccctcctc
      781 gaccggccca gtgaagaact cgaagcggcc acccttcatg ggcgcggggt cattcaccat
      841 caggacgtag tcgaaggccg tggcatcccg atgccactga tcgacattca gcgcgaggtc
      901 gtccggcgcg tagttgatgt gcaccgcgtg gtggctgatc gggtgcggct cgaggctggt
      961 gccggccagc tcgctgagga acgcgaggat ctcggggtcg tgatacatgc cgtagaggaa
     1021 ctccgaccgg tagacggagc cacgcatctt cttcgggatg cgccgtgtgc ccactgcgta
     1081 ctgctcgagc tccgcacaga tgcgctggag ggtctcgacg ccctcgtcgc tgagaaagcg
     1141 gaaagggctc gtaatcgcca gaggtgagag ggccgaggcg cctgctgcgc cttctcccca
     1201 ttcgtcgagc agcgtgatct tctcgggccg gacgagcttg cggtgtcggc tcggatccca
     1261 gggggtgtct ccgtcgagac gctcgtagcc gggcggtccg gccggaagtg cgctcatcgg
     1321 ctggtcatcg cggtgaaggc ggggagccag gcgacgctgt cggaaagccg gccgcccgcg
     1381 gcgacgaggt ctgcgatgag ctgcggccgg cgctcggcgt gcgatcgcga cgtcggcacg
     1441 cagatcgtca gggccgccag cacgacgccc tggccgttcg tgatcggaac cgcgaccgcc
     1501 cacaggccgg ctgccgtctc ctcgtcgttc acggcatacc ctcgctcgcg cacctcggca
     1561 agtatctccc ggagctcggc gaggccggcg agacgacgcc cggtgggcgt cacgagtggg
     1621 cggccgccgt agtcgccgac gaggttctcg ggtcgccgcg aggcgaggat cacacggccg
     1681 ctcgcgctgg cgaaggccgg cgtgcgcttc cccggacgtg cgacgaaacg aacgtcctcg
     1741 gaggtctcct ccacggcgac gagcaccgag tcgtccccgt cgatcgctgc tagacagacg
     1801 gtctcgtcat ggcgcgtgag aagctccgcc gctactga
//
LOCUS       DBNGNK010000170         6755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_459159,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000170 DBNGNK010000000
VERSION     DBNGNK010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_459159"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="AC150V_04900"
     CDS             complement(<1..508)
                     /locus_tag="AC150V_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3762294.1"
                     /translation="MSFDRPLLLLVLLVAPAVGLLYLLVQRRRRAYAIDFTNLQVLAA
                     VVERRSIWRSHLPSLLGALVLVALVMAVAGPFVTRDAPIERATVILAIDTSVSMLSKD
                     VKPTRLGAAKAAARAFVAEVPDRLRVGLVLFAGDVDVGAPPTADRDFALSTIAAIGPS
                     GVRSEERRG"
     gene            675..2315
                     /gene="pgi"
                     /locus_tag="AC150V_04905"
     CDS             675..2315
                     /gene="pgi"
                     /locus_tag="AC150V_04905"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR3762295.1"
                     /translation="MTTSATPTTPLRKRQAWAELQRHYETIRDVHLRELFATDPGRGE
                     RLTAEAAGLYLDYSKNRITDETVSLLVRLAEQSGLTERIAAMFRGERINVSENRPVLH
                     VALRMPKDASLVVDGVDVVAEVHEVLEQMASFANQVRTGTWKGHTGKPIRNIVNVGIG
                     GSDLGPVMAFEALRHYTRREMEFRFVSNVDSTDFVEATRDLAAEETLFVVSSKTFTTL
                     ETMTNAHSARDWCLASLRDEDAIARHFVAVSTNAKAVRAFGIDTANMFRFWDWVGGRY
                     SMDSAIGLSTMLAIGPEGFREVLAGFHELDEHFRTTPFERNLPVLMGLLAVWYGDFFG
                     AETAGVMPYSHYLKRFPAYLQQLTMESNGKHVTLDGRRVDYQTGAVYWGEPGTNGQHS
                     FYQLVHQGTKLIPVDLIGFAQSLNPLRDHHDILTSNVFAQAEALAFGKTEEEVRAEGT
                     PEEVVPHRVMEGNRPTNVLLLEQLTPRTLGSLVALYEHSVFTQGTIWGIDSFDQWGVE
                     LGKVLANRIVPELENEDEPELAHDSSTNALIRRYRSLR"
     gene            2403..3422
                     /gene="gnd"
                     /locus_tag="AC150V_04910"
     CDS             2403..3422
                     /gene="gnd"
                     /locus_tag="AC150V_04910"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015289585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR3762296.1"
                     /translation="MQLGMVGLGRMGANIVRRLMRDGHSCVVFDINPDAVKALEAEGA
                     VGATSLEDFATKLDQPRAAWVMVPAGDITERTISDLAAVLEVGDTIIDGGNTHYLDDL
                     RRSEELRGRGIHHVDCGTSGGVWGIDRGFCLMIGGEQEVVRRLEPLFASIAPGVDSAP
                     RTPGRDGPPGQSEHGYFHCGPNGAGHFVKMVHNGIEYGVMAAYAEGLAIIHAADAGKH
                     ERDADAETAPLEHPEHYRYEIDIPEVAEVWRRGSVIGSWLLDLTAAALHKSPDLSEFG
                     GRVSDSGEGRWTSVAAIEVGVPSPVLTTALYSRFSSRGADDFANRLQSAMRKEFGGHD
                     EKPPS"
     gene            complement(3439..4092)
                     /gene="rpiA"
                     /locus_tag="AC150V_04915"
     CDS             complement(3439..4092)
                     /gene="rpiA"
                     /locus_tag="AC150V_04915"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HIR3762297.1"
                     /translation="MTPVEREKQLAAEVAAELVESGMTLGLGTGSTVAYLLPALAARR
                     LELRCVATSPATATLARQLGIQVEPFEGTSALDQLDLAIDGADQVAPSGWLVKGGGAA
                     HTREKQVAAAAERFVVIVSSDKLVDRRAPPVPLELHEYGLAATLRRLGSVRLRDAPPS
                     PDGGAIADYLAEFEDPDDLAVALAADPGIVQHGLFPADMVAEVVAARGDRIERIVPG"
     gene            complement(4089..4979)
                     /locus_tag="AC150V_04920"
     CDS             complement(4089..4979)
                     /locus_tag="AC150V_04920"
                     /inference="COORDINATES: protein motif:HMM:NF038012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3762298.1"
                     /translation="MAASLALLAALLFALAATLQQRGALSLPEVSLLHPSSVVRLGAR
                     PIWLVGTFALLVGYAAQAAALDRGRLVVIQPLLVTTILFALPLGWLLTQQRVGLREGL
                     AALAVIAGLALFVEFGDPAGGRQDAPGPEWIVAVALVAVLAGLLAWLGTKWGPGGRAA
                     AYGAAAGLLFGLSATLTKPTIEKLHGGLIEVLSDWRVYVLLGAGFVGFVLQQVSLGTG
                     RLAPSVATVSVVNPVLAVVLGALILDERLSRPTWHVVVAVLALGMALAATVVISFGRE
                     PGPPEQAASNRRGSEKMLRG"
     gene            complement(4982..5710)
                     /locus_tag="AC150V_04925"
     CDS             complement(4982..5710)
                     /locus_tag="AC150V_04925"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3762299.1"
                     /translation="MSREHPSRIRADAPGGDRPTVVLFDIDGTLISTGGAGAAAWRLA
                     FDDLYGVPADIGQFSDAGMTDPDVGRRTFTAVVGREPTSAELARVMTARLGHLHQTVA
                     DSDGYVVHPGVVELLPKLVEQGVPLGLTTGNVEAAAHIKLHRARLNRFFCFGGYGSDS
                     DDRTELTRCALRRAETVLGTEIDFARCVGVGDTPHDVEAGHGAGIKVVGVATGHFGVE
                     ELQDAGADIVLASLEEELPIEQLG"
     gene            5828..>6755
                     /locus_tag="AC150V_04930"
     CDS             5828..>6755
                     /locus_tag="AC150V_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762300.1"
                     /translation="MSSAGVILVDPYPRSLSQIFGSGGRSRLERLGDVVWHDGPPAND
                     ALVEEHLPHVVAVVGQTTLPADRLALAPRLRAVVNVEGNFLPNVDYEECFRRGIDVLC
                     TSPVFAQPVAELALGMALASARDIAGGDARMRAREEALFDEGLTADYFLLAGKTVGIL
                     GVGAIGRRLLQLLAPFRCTLLGHDPWIGASALAQLGVESVEPAALFSRSRVVFVAASA
                     TSENEGMVDRSLLERMEKGSVLVLVSRAALVVWDDLVEAAESRHIRAAIDVFPTEPVP
                     PDERLRDIPGVVLSPHRAGNVPEVWPLIGEMVA"
BASE COUNT         1115 a         2341 c         2314 g          985 t
ORIGIN      
        1 ctccacgacg ctcttccgat ctaacgccgg acgggccgat cgctgcgatg gtcgacagcg
       61 cgaagtcgcg gtcggccgtc gggggcgcgc cgacgtcgac gtcgccggcg aagagcacca
      121 ggccgacccg gagtcgatcc ggcacctcgg cgacgaacgc ccgggcggcg gccttcgccg
      181 ccccgagccg cgtcggcttc acgtccttgc tcagcatcga cacggacgtg tcgatcgcca
      241 ggatcaccgt cgcccgctcg atcggggcgt cgcgcgtgac gaacgggccc gcgactgcca
      301 tgacgagcgc gacgagcacc agggcgccga gcagcgacgg cagatggctt ctccagatcg
      361 accggcgttc gaccacggcg gcgagcacct ggagattcgt gaaatcgatt gcgtacgctc
      421 gccgccgtcg ctggacgagc aggtagagaa gaccgacggc cggagccacc agcaggacga
      481 gcaacagaag gggccggtcg aagctcatcg cgtaggagtc ccgccgcggc ccgcggcggc
      541 ctgtccaccg ttatacaggc cgcggggtgg gccgggcggg ggcgccgcgt gccggggacg
      601 gcgtcggcgg ccatagggcg tcatctttcc tgggaagatg agagcccctg cccgattccg
      661 acggtgagga gacgatgaca accagcgcca cgcccacaac accgctgcgc aagcgccagg
      721 cctgggcgga gcttcagcgg cactacgaga cgattcgcga cgtccatctg agagagctct
      781 tcgcaaccga tcccggccgc ggcgagcgcc tgaccgcgga ggcggcggga ctgtatctcg
      841 actactcgaa gaaccggatc accgacgaaa cggtgagcct cctcgtgcgg ctcgccgagc
      901 agtcggggtt gacggagcgg atcgcggcga tgttccgggg cgagcggatc aacgtctccg
      961 agaaccggcc cgttctccac gtggccctgc gcatgccgaa ggacgcgtcg ctcgtcgtgg
     1021 acggcgtcga cgtcgttgcc gaggttcacg aggttctcga gcagatggcc tccttcgcca
     1081 accaggtccg gacgggaacg tggaagggac acacgggcaa gccgatccgc aacatcgtca
     1141 acgtcggcat cggcggctcc gacctcggcc ccgtgatggc attcgaagcg ctgcgccact
     1201 acacccgccg ggagatggag ttccggttcg tctcaaacgt cgactcgacc gatttcgtcg
     1261 aagcgacgcg cgacctcgca gccgaggaga cgttgttcgt cgtctcctcg aagaccttta
     1321 cgacgctgga gacgatgacg aacgcgcatt cggcacgcga ctggtgcctc gcgtcgctac
     1381 gcgacgagga cgcgatcgcc cgccacttcg tggcggtctc gaccaacgcg aaagccgtgc
     1441 gtgccttcgg catcgacacc gcgaacatgt ttcggttctg ggactgggtc ggcgggcggt
     1501 actcgatgga ttcggcaatc gggctctcca ccatgctcgc gatcggaccc gagggcttcc
     1561 gggaggtgct cgcaggcttc cacgagctcg acgagcactt ccgcacgaca cccttcgagc
     1621 ggaacctgcc ggtgctgatg ggactgctcg ccgtgtggta cggggacttc ttcggcgcag
     1681 agacggccgg cgtgatgccg tacagccact acctcaagcg cttccccgcg tacctacagc
     1741 agctgacgat ggagtcgaac ggcaagcacg tgacactcga cggccgccgc gtcgactacc
     1801 agaccggcgc cgtctactgg ggcgagccgg gcacgaacgg ccagcacagc ttctaccagc
     1861 tcgtccacca gggcacgaag ctgatcccgg tcgacctgat cggcttcgca cagtcgctca
     1921 acccgctccg cgaccaccac gacatcctca cctcgaatgt ctttgcgcag gccgaggcgc
     1981 tcgcgttcgg caagacggag gaggaggtcc gggccgaggg gacacctgag gaggtcgtcc
     2041 cgcaccgggt gatggaaggc aaccgcccga cgaatgtgct gctcctcgag cagctcacgc
     2101 ctcgtacgct cgggtcgctc gtcgctctct acgagcacag cgtgttcacg cagggcacga
     2161 tctgggggat cgattcgttc gaccagtggg gtgtggagct cggcaaggtg ctcgcgaacc
     2221 ggatcgtccc cgagctcgag aacgaggatg agccggagct cgcgcacgac agctcgacga
     2281 acgcgctgat ccggcgttac cgctcgctgc gttgaggggt tcgcgccgcg gctgtagcgt
     2341 gacctcttcc agcagcggaa tgcactccgt cagaaggagc ggacatgacc actgagaacc
     2401 ccatgcaact cggaatggtc ggactcggcc ggatgggcgc gaacatcgtg cgacggctga
     2461 tgcgcgacgg ccacagctgc gtcgtgttcg acatcaaccc ggacgcggtg aaggcgctcg
     2521 aggccgaagg agcggtcggc gccacctcgc tcgaggactt cgcgacgaag ctggaccagc
     2581 cacgcgccgc ctgggtgatg gttccggccg gcgacatcac ggagcggacg atcagcgacc
     2641 tggcggcggt gctcgaagtc ggcgacacca tcatcgacgg cggaaacacg cactacctgg
     2701 acgatctgcg ccgttcggag gagttgcgcg ggcgcggcat ccaccacgtc gactgcggca
     2761 cgagcggtgg ggtctggggg atcgaccgcg gcttctgcct catgatcggc ggcgagcaag
     2821 aggtcgttcg acggctcgag ccgctgtttg cgtcgatcgc ccccggcgtc gactccgccc
     2881 cgcgcactcc gggacgcgac ggcccgcccg gccagtccga gcacgggtac ttccactgcg
     2941 gcccgaacgg cgccggccac ttcgtcaaga tggtgcacaa cggcatcgag tacggcgtca
     3001 tggccgcgta tgccgagggg ctggcgatca tccacgccgc cgacgccgga aagcacgagc
     3061 gcgacgccga tgccgagacg gctccgctcg agcatcccga gcactatcgg tacgagatcg
     3121 acattccgga ggtcgccgag gtgtggcggc ggggcagcgt gatcggctcg tggctcctcg
     3181 acctgacggc tgcggcgctg cacaagtccc ccgatctctc cgagttcggc ggccgcgtct
     3241 cggactccgg agagggccgc tggacgtcgg tcgccgcgat cgaggtcggc gtaccgtctc
     3301 cagtcctcac caccgctctg tactcgcggt tcagctcacg aggagcggac gacttcgcga
     3361 accggctgca gtcggcgatg cggaaggagt tcggcggcca cgacgagaag ccgccgtcgt
     3421 gacgtccgaa gctgcgggct agccgggcac gatccgctcg atccggtcac cgcgagcggc
     3481 taccacttcg gccaccatgt cggccggaaa gagaccgtgc tgaacgatgc ccgggtccgc
     3541 cgccaacgcc accgcgaggt cgtccggatc ctcgaattcg gccaggtagt ccgcgatcgc
     3601 gccgccgtcg ggactgggcg gagcgtcacg cagccgcacc gagccgagcc gccgcagcgt
     3661 tgcggccaga ccgtattcgt ggagctcgag cggcaccggg ggcgcccggc gatcgaccag
     3721 cttgtcggag gagacgatca cgacgaaccg ctcggcggcc gccgccacct gcttctcccg
     3781 cgtatgcgcg gcgccgccgc ccttcacgag ccaacctgac ggagctacct gatcggctcc
     3841 gtcgatggcc agatcgagtt gatcgagcgc ggacgtgccc tcgaacggct ccacctggat
     3901 acccagctgt cgggcgagcg tcgccgttgc cggcgacgtg gctacgcacc gcaactcgag
     3961 ccgccgcgcg gccagtgcag gcagcaggta cgcgaccgtc gagccggttc cgaggccgag
     4021 cgtcatcccg ctttccacga gctctgccgc cacttcggcg gcgagctgct tctcacgttc
     4081 cacgggcgtc atccgcggag catcttctca ctcccacggc ggttggaggc cgcctgctcg
     4141 ggcggccccg gctcgcggcc gaaggagatg acgacggttg ccgcgagagc catgccgagc
     4201 gcgagcacgg cgaccacgac gtgccaggtt ggccgactca ggcgctcgtc gagaatcagg
     4261 gccccgagga cgacggcgag cacggggttc acgaccgaga ccgttgcgac ggagggcgcg
     4321 agccgtccgg tgccgagaga cacctgctgc aggacgaagc cgacgaaacc cgcgccgagc
     4381 agcacgtaca cgcgccagtc cgagagaact tcgatcagac cgccgtggag cttttcgatc
     4441 gtcggcttgg tcagggtggc cgagagcccg aacaggagtc ccgctgcggc gccgtatgcc
     4501 gccgcccgcc ctcctggccc ccacttcgtg ccgagccatg ccaacagccc ggccaggacg
     4561 gcgacgagag cgacggcgac gatccactcg gggcccggag cgtcctgccg gccgccggca
     4621 gggtcgccga attccacgaa gagagctagg ccggcaatga ccgcgagcgc cgccagccct
     4681 tcgcgcagcc cgacccgctg ctgcgtcagc agccacccga gcggaagcgc gaagaggatc
     4741 gtcgtgacga gcagcggctg gatgacgacg agacgtcctc ggtctagtgc tgcggcctga
     4801 gccgcatagc cgacgaggag ggcgaaggtt ccgacgagcc agatcggtcg tgcgccgagt
     4861 cgtacgaccg aggacggatg gagcagcgag acctcgggga gactcaaagc tccccgctgc
     4921 tgcagcgtcg cggccagagc gaagagcagt gcggccagga gcgcgagcga agcggccacg
     4981 atcagccgag ctgttcgatg ggcaactcct cctccagcga agcgaggacg atgtccgccc
     5041 cggcgtcctg tagctcttcg accccgaagt ggccggtcgc gacgccgacc accttgatgc
     5101 ctgctccgtg tcccgcctcg acatcgtgag gcgtgtcacc cacaccgacg cagcgggcga
     5161 agtcgatctc cgtgcccagc accgtctccg cccgccggag ggcgcaccgt gtcagctcgg
     5221 ttcgatcgtc ggagtcggag ccgtagccac cgaagcagaa gaaccggttc agccgtgccc
     5281 ggtgcagctt gatgtgcgcg gcagcctcga cattccccgt ggtcaggccc agcgggacgc
     5341 cctgctcgac cagcttgggg agcagctcga cgacacccgg gtggacgacg tagccgtccg
     5401 agtccgcaac ggtctggtgg aggtgcccga gccgggccgt catcacccga gccaactcgg
     5461 ccgaggtcgg ctcgcgaccg acgacggcag tgaaggtgcg acgtccgacg tctgggtcgg
     5521 tcatgcctgc gtcggagaac tgcccgatgt cggcgggaac tccgtacaga tcgtcgaaag
     5581 cgaggcgcca cgccgcggcg cccgctccgc ccgtgctgat cagcgtcccg tcgatgtcga
     5641 agaggacgac ggtcggccgg tcgccgccgg gagcatcggc tcggattcga ctggggtgtt
     5701 ccctgctcat gggccggtgc ggcaacagac gccgcggaag ccacgaacct atacaaccga
     5761 gaggaaagca cgcctgcgta tgccgctcgc gcgctccggc gccgagcggg gtgagatgct
     5821 cccgacgatg tcttcggccg gcgtgatcct cgtcgatccg tacccgcgct cgctctcgca
     5881 gatcttcggc agcggcggac ggtctcggct cgagcggctc ggcgacgtcg tctggcacga
     5941 cggcccgcct gccaacgatg cgctcgtcga ggagcacctc ccccacgttg tcgccgtcgt
     6001 cggccagaca acccttcccg ccgaccgcct cgcgcttgcg cctcgcctcc gcgccgttgt
     6061 caacgtcgag ggcaacttcc tgcccaacgt cgactacgag gagtgcttcc gacgcggcat
     6121 cgacgtgctc tgcacctcgc cggtgttcgc gcagcctgtg gcggagctcg ctctggggat
     6181 ggccctcgcc tcggcccgcg acatcgccgg cggcgacgcc cgcatgcgtg cccgcgaaga
     6241 ggctctcttc gacgagggtc tgaccgcgga ctacttcctg ctcgcgggca agaccgtcgg
     6301 gatcctcggc gttggagcga tcgggcgtcg gctgctccag ttgctcgctc cgttccgatg
     6361 cacgctgctg ggccacgacc cttggatcgg cgcgtcggcg ctggcgcaac tcggcgtgga
     6421 gtccgtcgag ccggcggcgc tcttctctcg cagccgcgtc gtgttcgtcg ccgcctcagc
     6481 gacatccgag aacgagggca tggtcgaccg cagcctgctc gagcgcatgg aaaagggctc
     6541 cgtcctcgtg ctcgtatcgc gggcggcact cgtcgtctgg gacgatctcg tcgaagcggc
     6601 ggagagccgg cacatcagag cggcgatcga cgtgttcccg accgagcccg ttccgccgga
     6661 cgagcgtttg agagacattc cgggcgtcgt cctctcgccg catcgagccg gcaacgtccc
     6721 cgaagtgtgg cccctcatcg gcgagatggt cgcgg
//
LOCUS       DBNGNK010000171         3061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_529842,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000171 DBNGNK010000000
VERSION     DBNGNK010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_529842"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..650)
                     /locus_tag="AC150V_04935"
     CDS             complement(72..650)
                     /locus_tag="AC150V_04935"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3762301.1"
                     /translation="MPRAAPLSVSSDVPRIGARIRALRRERGLTIEQVAAATGLTKGF
                     ISQLERDRTQPSLSSIARICDALGVRLSHIFERGPSPALVRRGERSEIDTYFPTSNYL
                     LSSRDEQRFQAIESVVAPGAGAGDELYTLPGEVELVYVLEGVLELRVGEETHLLEEGD
                     ALTYELRKPHTWRNPSADRTLRVLWVAVPNPY"
     gene            764..3052
                     /gene="lon"
                     /locus_tag="AC150V_04940"
     CDS             764..3052
                     /gene="lon"
                     /locus_tag="AC150V_04940"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR3762302.1"
                     /translation="MTKLLLVPLEDTVVFPNMSVTLTVDVGDEERVLLVPKHDGEYAA
                     VGTVAEVTDRARLPGGGRAVALNGLHRGIAGAAEPDSAGRLFVDVEESPDDEPTDAAT
                     RDLEREYRAVVEEILDLRGDDGRIAAFVRSIVEPGALADTSGYSPDLTFEQKVELLET
                     VDVKERLELALGFQRERLTLMQLRKRIRDDVNEGVEQQQREYILRKQLESIRKELGED
                     EGSVVDEYRKKIAESGMPDGVREQAEKELGRFERMGENSPESSVIRNYLDWLLAVPWG
                     ERSEERLDPAYAREVLDADHAGLEDVKERIVEYIAVAKLRRERGIEADRRSGAILTLI
                     GPPGTGKTSIGESVAKALGREFVRMSLGGVRDEAEIRGHRRTYIGALPGRLVRALRDA
                     GTMNPVILLDEVDQVGADWRGDPSAALLEVLDPAQNHSFRDHYLDVEVDLSEVVFIAT
                     ANVAESIPGPLLDRMEVIRFDGYTTDEKVAIANGYLWPRQRERAGLHEDEVAVSNEAV
                     ETVISEYTREAGVRQLERELGSILRKTATGIATGDIEPPVDIDVDAVRGALGRQKFFR
                     EAAERTAVPGVATGLAVNATGGDVLFVEATSVGGGERLVLTGQLGDVMKESAQIALSS
                     VRSRADELGLDDEAFEDRSFHVHVPAGAIQKDGPSAGITMATALASLLSGRPVRHTVG
                     MTGELTLQGRVLPIGGLKQKVLAAHAAGLTDVVLPERNRADLDEVPEHVRDEMTFHPV
                     MSFSEVLDVALEPQSAALDVSL"
BASE COUNT          516 a          999 c         1069 g          477 t
ORIGIN      
        1 cctccgtcgc gttgtacgac ggacaaacgt ttgtttagtt agaacaaacc atcgtcggtc
       61 gaacttcggg gtcagtacgg gttcggcacc gcgacccaga gcacgcgcag cgtgcggtcg
      121 gccgacgggt tccgccacgt gtgcggcttg cgcagctcgt aggtcagcgc gtcgccttcc
      181 tccagcaggt gcgtctcctc gccgactcgc agctccagga caccctcgag cacgtagacg
      241 agctcgacct cgccggggag cgtgtagagc tcgtcgccgg caccggcgcc cggtgcgacg
      301 accgattcga tcgcctggaa gcgctgctcg tctcggctgg agaggaggta gttgctggtc
      361 ggaaagtacg tgtcgatctc cgaccgctcc ccgcgtcgta cgagcgcagg cgacgggccg
      421 cgctcgaaga tatggctcag ccgcacgccg agcgcgtcgc agatccgcgc gatcgacgag
      481 agcgacggct gcgtccggtc gcgctcgagc tggctgatga accccttggt caggccggtg
      541 gcggctgcaa cctgctcgat tgtcagccca cgctcacgtc ggagggcccg aatccgggct
      601 ccgatccgcg gcacgtccga gctcaccgac agcggggctg cgcgcggcac ccgtcgatct
      661 tacggcactt ccgacgccgc ggccgtgagc cggaatacag ctatcatttt tgtagttaat
      721 acccgtacaa gaccgctgaa ctgaaccgag cgaaggagtc gcgttgacca agctgctgct
      781 cgtcccactg gaagacaccg ttgtcttccc caacatgagt gtgaccctca ccgtcgacgt
      841 cggcgacgag gagcgcgtac tcctggtgcc caagcacgat ggggagtacg ccgccgtcgg
      901 gacggtcgcc gaggtcaccg atcgggcccg gctgccagga ggcggccgcg cggttgcgct
      961 caacggcctt caccggggca tcgccggcgc cgcggagccc gactcggcag gccggctgtt
     1021 cgtcgacgtc gaggagagcc ctgacgacga gccgaccgac gctgctacgc gagacctcga
     1081 gcgcgagtat cgagccgtcg tcgaggagat cctcgatctg cgcggcgacg acggtcgcat
     1141 cgccgccttc gttcgctcga tcgtcgagcc gggcgcactc gcagacacgt ccgggtactc
     1201 ccccgacctc accttcgagc agaaagtgga gctgctggag accgtcgacg tgaaggagcg
     1261 cctggagctg gcgctgggat tccagcgtga acgcttgacg ctcatgcagc tgcgcaagcg
     1321 aatccgtgac gacgtcaacg agggagtcga gcagcagcaa cgcgagtaca tcctgcgcaa
     1381 gcagctcgag tcgatccgca aggagctcgg cgaggacgag gggtccgtcg tcgacgagta
     1441 ccgaaagaag atcgccgagt cggggatgcc cgacggggtg cgtgagcagg ccgagaagga
     1501 gctcgggcgg ttcgagcgca tgggcgagaa ctcgcccgag tcgagcgtca tccgcaacta
     1561 cctggattgg ctcctggccg tcccgtgggg cgagcggtca gaggagcgcc tggatccggc
     1621 ctacgcgcgc gaggtcctcg acgcagacca cgccgggctg gaggacgtca aggagcgaat
     1681 tgtggagtac atcgccgtcg cgaagctgcg ccgggagcgc ggcatcgagg cggaccggcg
     1741 ctccggagcg atcctcacct tgatcggccc gcccggcacg gggaagacat cgatcggaga
     1801 gtccgtcgcg aaggcgttgg ggcgcgagtt cgtccgcatg tcgctcggtg gtgtccgtga
     1861 cgaggccgaa atccgcgggc atcgccgcac gtacatcggc gcgctgcctg ggcggctcgt
     1921 acgcgcgttg cgcgacgcgg gaaccatgaa ccccgtgatc ctgctcgacg aggtcgacca
     1981 ggtgggcgcc gactggcgcg gtgacccctc ggccgcactg ctcgaggtcc tcgacccggc
     2041 gcagaatcac tcgttccgcg accactacct cgacgtcgaa gtcgacctgt cagaggtcgt
     2101 gttcatcgcg accgccaacg ttgccgagtc cattccgggc ccgctgctcg accggatgga
     2161 ggtgatccgg ttcgacgggt acacgacaga cgagaaggtc gcgatcgcga acgggtacct
     2221 ctggccccgg cagcgtgagc gtgcaggcct gcacgaggac gaggttgcgg tgtcgaacga
     2281 ggcggtcgag acggtcatct ccgagtacac ccgcgaggcc ggcgtccggc aactggagcg
     2341 cgagctcggc tcgattctcc gcaagacggc gacgggcatc gcaaccggcg acatcgagcc
     2401 accggtcgac atcgacgtcg atgcggtgcg aggggcgctt ggtcggcaga agttcttccg
     2461 cgaggcggcg gagcgaacgg ccgttccagg agtggcgacc ggtctcgccg tgaacgcgac
     2521 cggcggcgac gtccttttcg tcgaggcaac gagcgtcggc ggcggcgaaa ggctcgtcct
     2581 gacgggccag ctcggagacg tgatgaagga gtcggcgcag atcgctctct cctcggttcg
     2641 gtctcgagcc gacgagctcg ggctggacga cgaggccttc gaggacaggt ctttccatgt
     2701 ccacgtgcct gccggggcga tccagaagga cggcccgagc gccggtatca ccatggcgac
     2761 cgcgcttgct tcgctgctct cgggcaggcc ggtcaggcac accgtcggaa tgaccggaga
     2821 gctcaccctg cagggccgag tgctgccgat cgggggcctg aagcaaaagg tgctcgcggc
     2881 acacgcggcg gggctgaccg acgtcgtcct ccctgagcgc aatcgcgccg acctcgatga
     2941 ggttcccgag catgttcgcg acgagatgac gttccacccg gtgatgtcgt tctccgaggt
     3001 tctcgacgtc gcgctcgagc ctcagtccgc ggcgctcgat gtctcgctgt aagcgctgcg
     3061 c
//
LOCUS       DBNGNK010000172         2647 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_745504,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000172 DBNGNK010000000
VERSION     DBNGNK010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_745504"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /locus_tag="AC150V_04945"
     CDS             complement(<1..94)
                     /locus_tag="AC150V_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3762303.1"
                     /translation="MEEEVIPVLYVEDAVRAVAWYERLGFRKEWE"
     gene            553..>2647
                     /locus_tag="AC150V_04950"
     CDS             553..>2647
                     /locus_tag="AC150V_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3762304.1"
                     /translation="MATSPVIGRQRELKVLRSFVARIPAGPAVALLEGEAGAGKTTLW
                     RAAADAAEERGVRSLRSRPTESESAMSFVALGDLLEDVLDDVLPQIPAPQARALSHAL
                     LLEEDGASLGARALGVAFLNILGVLARDAPLLVAVDDVQWLDVESAAILAYSGRRLRE
                     EPVALLLARRVGLPSPLIDDLDRSLPAERLFRVQVGALRQEDVGRLLAQRVGLQLPRP
                     LLAQIYETSGGNPFYALELARTLDHDGGFVRAGSPLPVPETLRELVFGRLASLPAECR
                     LPLLAVAALSQPTLRLVAAAAGPHAGADIRPALEAGVLELDGNRLRFSHPLLAAGAYE
                     SADPIRRAELHARLAELVTDPEERARHLAAAADGPDERVAGALEDASRRAEARGAPRG
                     AAHLLEQAHALTPPEHADDRARRVVDAAYHHFEAGDSRRAAAMLEEVIAELTPGRLRA
                     RALIRLARVRSYEEQALGARLFRQAIDEADGDRKVLAVAHEGVASCLFRLREQLAEAV
                     DHAALAVQLALELGDQQLAAEALASSLLAEAALGRRSAIRTLERVLEAQQTADNSRVL
                     GQPLTMAGVYWWWSDDLARARETFEHALARSQALGDESSRPYILVLLGQVECSLGELA
                     SAARRAAEGRELAEQAGQATVLAYHLGLASLVAALRGDARAARQSGRRALELAPETGG
                     RPAEMLARTALGRLEL"
BASE COUNT          373 a          932 c          897 g          445 t
ORIGIN      
        1 gctcccactc cttgcggaat ccgaggcgct cgtaccaagc gacagcgcga actgcgtcct
       61 cgacgtagag gactggaatg acctcttcct ccaccctacg ccgataatac gccccgccac
      121 gcggcacaac ctggaccgta tgggcgcctt ccagtcacga ctttcgcctg cgtcagcgtg
      181 ctgtacctag aaatttccac ctagaaattt cccccattcg gctacgacct ccaaggagtg
      241 gccggggaca cttcaaggac gcggcgagcc tcggtcgcct tccgatcgac gtgaacgccg
      301 ccggaggcag cgacgatctc gtagtctcgc tccgcgtccg taacgccgat gtatccgtgt
      361 acgagatcct cttcgatctg cccgaattcc cgctcgatac ctggcggttg acccagttcg
      421 tcatcttccg gcaccggcga cccggcaggg gtgggctctg gctaagccct cccacatctt
      481 catgcaagcc acccgtctcg agcggggcgc gagaggatcc gcccgtcgtc ggcacggcac
      541 aattgccacg agatggcgac gtccccggtc attgggcgcc aacgcgaact aaaggtcctg
      601 cgatcgttcg ttgcgcgcat cccggccggg ccagccgtcg ctctgctcga gggcgaggcg
      661 ggggcgggaa agacgacgtt gtggagagcc gcggctgacg ccgccgagga acgaggcgtt
      721 cggtcgcttc ggagccgacc gaccgagagt gagtcggcga tgtccttcgt agccctaggc
      781 gatctgctcg aggacgtgct cgacgacgtg ctcccccaga tcccggcacc gcaggcccgt
      841 gcgctctccc acgcgcttct gcttgaagaa gacggtgctt cactcggcgc acgagcgctc
      901 ggagtggcct ttctcaacat cctcggcgtc cttgcgcggg atgcgccgct gctcgtcgcc
      961 gtggacgatg tccagtggct cgacgtcgag tcggccgcga tcctcgccta ctcgggccgc
     1021 cggctgcggg aagagccggt tgccctcctg ctcgcgcgac gcgtcggcct gccgagtccg
     1081 ctcatcgacg acctcgatcg ctctctgccg gccgaacggc tctttcgtgt tcaggtaggc
     1141 gcgctcagac aagaggacgt cgggcggctg ttggcacagc gggtcggtct gcagctgccc
     1201 cggccgctgc tggcccagat ctacgagacg tcgggcggga accccttcta cgccctcgag
     1261 ttggcgcgga cgctcgacca tgatggtgga ttcgtccggg ccggcagccc tctgccagtg
     1321 cctgaaacgt tgcgtgagct cgtcttcggg cgcctcgcct cgctcccggc cgagtgccgg
     1381 ctgccgctcc tcgccgtggc ggcgctttcc caaccgacgc tgcgcctcgt cgcggccgcg
     1441 gcgggaccgc atgccggggc ggacattcgc ccggccctcg aagcgggcgt gttggagctg
     1501 gacggaaacc gcctccgctt ttcgcacccc ctcctcgcag ccggggcgta cgagtccgcc
     1561 gaccccattc ggcgtgcgga gctgcacgcc cgcctcgcgg agctcgtgac cgatccggag
     1621 gagcgcgcgc gccatcttgc tgccgccgcg gacggaccgg atgagcgggt cgccggcgct
     1681 cttgaagacg cgtcgcgtcg ggcagaggcc cgcggcgcac cgcggggggc cgcgcatctg
     1741 ctcgagcagg cgcacgctct cacgccgccg gagcatgcgg acgaccgggc ccggcgggtc
     1801 gtcgacgctg cctaccacca tttcgaggcc ggcgactccc ggcgcgccgc cgccatgctt
     1861 gaggaggtta ttgctgagct tacgcctggc cgtttgcgcg ctcgtgccct gattcgactc
     1921 gcgcgtgtcc gttcctacga ggagcaggcg ctaggcgccc gcctcttccg ccaggcgatc
     1981 gacgaggctg acggcgatcg aaaagtgctt gccgtcgccc acgaaggagt tgcttcatgc
     2041 ctcttccggc tgcgagagca gctggccgag gcggtcgacc acgccgcgct ggctgtgcag
     2101 ctcgcgctag agctgggcga tcagcagctc gcagccgaag ctttggcctc tagtctgctt
     2161 gcggaggcgg cgctcggccg gagatcggcg attaggacgc tggaacgagt actcgaggcc
     2221 cagcagacgg cggacaactc gcgcgtactc gggcagccgc tcaccatggc cggcgtgtac
     2281 tggtggtggt cggacgatct cgctcgcgcg cgcgagacct tcgagcacgc tctcgcgcgc
     2341 tcgcaggcgt tgggcgacga gagctcgcga ccgtacatcc tcgtcttgct cggacaggtg
     2401 gagtgcagtc tgggtgagct cgcgagtgcg gcgcgccgag cggccgaggg acgcgagctc
     2461 gccgagcagg cgggccaagc gactgtgctc gcgtatcacc tcgggctcgc gagcctcgtc
     2521 gccgcgctcc gtggcgatgc cagagccgct cgccagtcgg gtcggcgggc gctggagttg
     2581 gcccccgaga cgggcggtcg tccggccgaa atgctcgccc ggactgcgct cggccggctc
     2641 gaacttg
//
LOCUS       DBNGNK010000173         2181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_244657,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000173 DBNGNK010000000
VERSION     DBNGNK010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_244657"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="AC150V_04955"
     CDS             <1..729
                     /locus_tag="AC150V_04955"
                     /inference="COORDINATES: protein motif:HMM:NF025585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HIR3762305.1"
                     /translation="RPGAKFVKVSKGNGVKAYAGLRDDPFFFDLAGYLDILTAIDDDT
                     TNNDKSFIGCKAPRADFFKGLNVSAIVLELPPSLLTADDSTAIGVWGATTVGKKQVDR
                     IGRPAISTVFVPNNPFPGEQQGEVRSKKAAYNKGQPKDDQKAFRGEIVNTLGVLFSLN
                     DAAGDDASDDAKQIGDLADILLPDILTFDTAKSDGFLNGRRLADDVIDAELGLITEGL
                     VTTDCVSKNDRKFWPNFPYLATPH"
     gene            823..2154
                     /locus_tag="AC150V_04960"
     CDS             823..2154
                     /locus_tag="AC150V_04960"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3762306.1"
                     /translation="MTLRRSSRLLLLAAATVAIATVALFGRGGDSAPARSAESEMTAV
                     DVGKLVSAFSAGDSADVVRRLERRASDRPQDAVLQTYLGLGYQQLFRETGRAAWLSRS
                     RSALERAVELGATRDAVTLAGLAQLSATQHRFREAVRHARAALRVAPGNPVAVGALGD
                     GLVELGRYEEAFAVYDRLASQGPSVAGYARIARARELLGDQNGALEALELAVEAGSGI
                     PEQEAWALSRRGELLLQTGRVDAARAAFGRSLALSPNFPHARAGLAKADLADNRPRAA
                     ARALEALLRAVPSAEYATELGDVYLSLGAKRPADAAYDRARRFEQTLARHGVRTMLAS
                     ARLDLDLGTRLASALVRARQAYREAPNIETLDLMAWALERNGRCREALTWSTRSLAIG
                     TKDPTLYFHRGMIERCLGRDRTAVGWFRQALELDPAFSVRWAPLATRLASA"
BASE COUNT          348 a          764 c          720 g          349 t
ORIGIN      
        1 cgtcctggtg cgaagttcgt gaaggtcagc aagggcaacg gcgtcaaggc ctacgcgggc
       61 ctgcgcgacg acccgttctt cttcgacctc gccggatacc tcgacatcct cacggctatc
      121 gacgacgaca cgacgaacaa cgacaagtcg ttcatcggct gcaaggcgcc gagggccgat
      181 ttcttcaagg gcctcaacgt ctccgccatc gttctcgagc ttcccccgtc gttgctgacg
      241 gcggatgatt cgacggcgat cggcgtctgg ggtgcgacca ccgtcggcaa gaagcaggtc
      301 gaccgaattg gtcgcccggc gatcagcacg gtcttcgtcc cgaacaaccc gttccccggc
      361 gagcagcagg gcgaagtgcg gtcgaagaag gcggcctaca acaaggggca gccgaaggac
      421 gatcagaagg cgttccgtgg cgagatcgtc aacaccctcg gggtgctctt cagtctcaac
      481 gatgcagcgg gtgacgacgc gagcgacgac gcgaagcaga tcggcgacct cgccgacatc
      541 ctgctgcccg acatcctcac gttcgatacc gcgaagtcag acggtttcct caacggtcgc
      601 cggctggccg atgacgtgat cgacgccgag ctcggtctga tcaccgaggg cctggtcacc
      661 acggattgcg tgtcgaagaa cgaccgcaag ttctggccga acttccccta cctggcaact
      721 ccccactagc catccacgac gaccagacga ccacggcgag ggcggcgcca gacgccctcg
      781 ccgtgggccg aaccgaagtc gacaaaccca cgaaggagac acatgacctt gcgacgatcg
      841 tcccgcctgt tgctactcgc cgccgcgacc gtggcgatcg cgacggtcgc tctcttcggc
      901 cggggcggcg attctgctcc cgcgcgctcc gcagagtccg agatgaccgc cgtcgacgtc
      961 ggcaagctgg taagcgcgtt ctcggccggc gacagcgccg acgtcgtgcg ccgcctggag
     1021 cgtcgtgcct cggatcgccc acaggacgcc gtgctccaga cgtatctcgg cctcggatat
     1081 cagcaactgt tccgcgagac cgggcgcgcc gcctggctgt cgcgctcccg cagcgccctc
     1141 gagcgcgcgg tcgagctcgg cgcgacgcgc gacgcggtga cgctcgccgg cctcgcccag
     1201 ctgtctgcga cgcagcatcg cttccgcgaa gcagtccgtc acgcccgcgc ggcactgcgg
     1261 gtcgcgcccg gaaatcccgt ggccgtcggg gctctcggcg acggactcgt cgagctcggt
     1321 cgttacgagg aggcctttgc cgtgtacgac cgcctcgcgt ctcaggggcc gagcgttgcc
     1381 ggctacgcac ggatagctcg tgcgcgagag ctgctcggag accagaacgg agccctcgag
     1441 gcgctcgagc tcgccgtcga ggcaggctcg ggcatccctg agcaggaagc gtgggcgctg
     1501 agccgccgcg gggagttgct gctccagacc ggtcgcgttg acgcggctcg agccgccttc
     1561 ggccgctccc tggccctcag tcccaatttc ccgcacgctc gcgccggtct tgcgaaggcc
     1621 gatctggcgg acaatcgccc gcgggctgcg gcgcgtgcct tagaagcact gcttcgcgcc
     1681 gttccgtcgg cggaatacgc gacagagctc ggcgacgtct atctctcctt gggcgcgaag
     1741 cgcccggcgg atgccgccta cgaccgggca cgcaggttcg agcagaccct ggcccgccac
     1801 ggcgtcagga cgatgctcgc ctctgctcgg ctcgacctcg acctgggcac gcgtctcgcc
     1861 tcggctctcg tccgggcgcg gcaggcgtac cgggaggcac ccaacatcga gacgctcgat
     1921 ctgatggcat gggcgctcga gcgcaacggt cgctgtcggg aggcgctcac ctggtcgact
     1981 cgttcgctgg cgatcggcac gaaggatccg acgctctact tccatcgcgg catgatcgag
     2041 cgatgtctgg gacgagatcg aaccgccgtc ggctggttcc ggcaggcgct cgagctcgac
     2101 ccagcgttct ccgttcgctg ggcgccgttg gcgacgagac tcgcgtctgc gtgaccagct
     2161 gtagggttgt cgcgtggacc c
//
LOCUS       DBNGNK010000174         8724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1964261,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000174 DBNGNK010000000
VERSION     DBNGNK010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8724
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1964261"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC150V_04965"
     CDS             complement(<1..621)
                     /locus_tag="AC150V_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3762307.1"
                     /translation="MSERHQGGRLVARAVAAEGVDTIFTLSGGHVMPIYEGCRHEGVA
                     VVDVRHEQSAAHAAEAWGRVRRTAGVAVVTAGPGVTGTVTAVAAALAGQAPLVVIGGA
                     RPLVQAEQGALQEFDQLSLMKPITKWAAVCASTERIPEYVSIAFRHALSLPRGPVYLE
                     VPMDVLFDEATAEPQPSARVSSAPSPSPQQIDRAAELLAGAERPALV"
     gene            662..1642
                     /gene="rnz"
                     /locus_tag="AC150V_04970"
     CDS             662..1642
                     /gene="rnz"
                     /locus_tag="AC150V_04970"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007690838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR3762308.1"
                     /translation="MDLDVVFLGTSGSTPTARRSAAATLVRRGGERLLVDCGEGTQRQ
                     LLRSDIGLVELEEVFLTHYHADHYLGLPGMLKTFALRGRDLPLTLYGPSGLRELLSTL
                     ARVFGRLSFPLETVELEPGDVLARSEYRIEAFAVHHGLPAVGYAFVEEERPGRFDVAQ
                     AERLGVPDGPLRGALQRGEEVTLEDGTSVRPEQVVGERRPGRKLALAGDTAPAASVVE
                     ASRGADLLVHEATFCAEERARARETLHSTAGEAALAAREAGVQLLALTHLSSRYAGPD
                     VEEEARQIFPASIVPRDFDVIRIPFSERGRPELTPRGARHGRARVGSAGE"
     gene            1639..1872
                     /locus_tag="AC150V_04975"
     CDS             1639..1872
                     /locus_tag="AC150V_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762309.1"
                     /translation="MTNQVQVAVAGDVTEAEELQTILHAAGIEATLEMAAEHEPGAMD
                     DPPLKVLVAANDVDAARDAIEALTEPDELIDED"
     gene            1998..2648
                     /locus_tag="AC150V_04980"
     CDS             1998..2648
                     /locus_tag="AC150V_04980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762310.1"
                     /translation="MREGDLKGRRVLDVGCGTGLLTAALAETGHAKVWGVEPSDEMLA
                     VARARVPRGVGLRRGAAEELSFRDGWFERVVFSLVVHLVDRPLAFAEAARVLSASGKV
                     ALASFAPEHFDVYWLNPWFPRIAEIDRARFPTPAELERELRASGFVGERFVRLTTRQE
                     IDRATALERIRGRHISTFDLLSEDELREGIARAEDELPDRVPGRLEQLIVIAERQP"
     gene            complement(2689..4755)
                     /locus_tag="AC150V_04985"
     CDS             complement(2689..4755)
                     /locus_tag="AC150V_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762311.1"
                     /translation="MLDGGRAWAARRPEVVAVLALGAMVVVSAGLRLLAARTFTVPWI
                     APDEMVYGLLGQDLWERGTLTIRGFDVPYHSLLYPAFVGLPLLAGELADGIRLAQALQ
                     ALVISLAAVPVYLWGRRFLGRRWALAAAALTLLTPALTYAGLLMTEALFYPATAVALA
                     ALARVLETPTLMRQGAFLLAVTFVAAIRLQALLLLPALVSAALLYALFVRSAKILRRL
                     WPILGILALGSLLAVTWVFVSDDAGLDDVVGAYAAGARPDSTALGWELVWHAGYVAIL
                     GAFLPFLATGTLVWRAARTGETDPPVAAFLAATASFVPLLAAQVALFAAENVDHVGGR
                     YLITALPLLALGVCLWGVRGAPWERIGTPVLAIAMVALVALTPIDQSAAAAGAHDLFE
                     TLVLRDLADATSDAIARIVLVAAAAFGVLAIVLVARRHDRYPAGASIAVLATGAVLAA
                     ASLVAALDVARLSEKARERAFGPAVTTWVDDAGDESSVLLATGELLWTSNARILFWNE
                     SIQRIVALPSAEWHGPLPVLSVTLSEQGEVIREDGTVLSASRAVAPSTLVLEGEALAV
                     AAMTTDEPEKTLWRMDGALRATTSRAGFTPVGDILGEAGIVVYGCKRGGALALTLLGK
                     EGKPINIGVNGIPVRTVTIEPEKVWRGSIPAPPDADGSRSCYYVLESPGLVGSTRVEW
                     IPPPTD"
     gene            4871..5464
                     /gene="pyrR"
                     /locus_tag="AC150V_04990"
     CDS             4871..5464
                     /gene="pyrR"
                     /locus_tag="AC150V_04990"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR3762312.1"
                     /translation="MTTHLPSRSAPATVLDAEAMSRTLSRIAHELIERSESLDEIALV
                     GIHTRGVPLAQRIRRLVSERSGIDLALGAVDITFHRDDVSVRAGGAPRHPQPVVRGTA
                     LDFPLEGMTCVLVDDVLHTGRTIRAAIDALLEYGRPERVELAVLVDRGHRELPIRPDY
                     VGKNLPTARNERVHVELVEVDEIDRVLLVAAEEDRDG"
     gene            5457..6443
                     /locus_tag="AC150V_04995"
     CDS             5457..6443
                     /locus_tag="AC150V_04995"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR3762313.1"
                     /translation="MAEIQLVSHLQPPDRPPRRHLLGVSDLSRADVERILRTARALER
                     SLERELKKLPTLRGRTVVNLFYESSTRTLSSFELAAKRLSADTMSVRAAGSSVDKGES
                     LKDTALTLAEYDPDVIVVRHPQIGAPHLVAATTQAHVVNGGDGKHQHPTQALLDLYTM
                     ERALGRLEGLRIAIVGDVLHSRVARSLIEALALVEAETTLVGPPTLMPRGIEAMGCSV
                     ASEIAAVEDADVIYVLRMQQERMLPGAAYVPSLREYSALWAITPERVRKGQVVMHPGP
                     MNRGVEIDPRVADSDAALITAQVRSGLVVRMAVLYDLLTSSPVELATRLEVA"
     gene            6440..7723
                     /locus_tag="AC150V_05000"
     CDS             6440..7723
                     /locus_tag="AC150V_05000"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR3762314.1"
                     /translation="MMLSCRNGRHDTLTIVGARVVDPCQDVDQSLDVTIEEGVITELQ
                     PTAAAVRGLVLAPAFVDPHVHLRTPGREDEETIASGTAAAAAGGYCAILAMPNTDPVV
                     DCAAVLGALIERAREEAMVPTGFLASISKGQRGEELSEFGDLAASGAVGFTDDGVPVA
                     STGLMRRALQYSAVADRPLALHEEEPSISRDGQMHEGVVSAELGLAGWPSVAESVMVQ
                     RDLALAAYEERPLHVMHVSARESVDALRIAHARGVQATAEVTPHHLVLTDDAVRSLDP
                     NVKMNPPVRSDDDRTTLIEALRDGTISCVATDHAPHARHEKEAPFEAAPFGVTGLETA
                     FAALYTYLVRPGVVPLETVLERMSAGPARAFGLPVPRIAVGEKANVVLLDLDSQWRVS
                     EQSFRSRCVNSWLLGHTLHGAVVKTVADGRVVHAA"
     gene            7720..>8724
                     /gene="carA"
                     /locus_tag="AC150V_05005"
     CDS             7720..>8724
                     /gene="carA"
                     /locus_tag="AC150V_05005"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR3762315.1"
                     /translation="MTASGYLLLEDGTVFRGRSVAAPGAVFGEAVFTTGMTGYQETVT
                     DPSYAEQLVAFTTAMVCNYGVDDARLESDRPHARGVLMRLCGGDAWAEWLAAAGMVAL
                     DEIDTRALTLRLRDDGAMRAVLVADEAELAVADALQQVRAQPGMEGAALVAQVSTREP
                     YTPIPEGRLRVAVVDYGAKRSIVRRLARAGAAVTVFPHVHDPDELASFDGVLLSNGPG
                     DPEPLSEETEVVRALLGRTSVLGICLGHQLLGLAAGHRTFKLPFGHRGANHPVLERRS
                     SRVLVTSQNHGFAVAASDDVEATYVSLYDGTVEGFDFAELRARSVQFHPEAGPGPHDA
                     W"
BASE COUNT         1323 a         3007 c         3135 g         1259 t
ORIGIN      
        1 gaccagcgcc ggccgttccg cccccgccag cagctccgcg gcgcgatcga tctgctgcgg
       61 agagggtgac ggggcagagc tcacgcgcgc cgacggctga ggctcggccg tcgcctcgtc
      121 gaacagcaca tccatcggca cctcgaggta gacggggcct ctcggcaggg agagggcgtg
      181 ccgaaaggcg atcgagacgt actcggggat gcgctcggtc gaggcacaca ccgctgccca
      241 cttggtgatc ggcttcatca gcgagagctg gtcgaactcc tggagagccc cctgctcggc
      301 ctggacgagg gggcgcgcgc cgccgatcac gacgagcggc gcctgacctg caagtgcggc
      361 ggcgacggcg gtgaccgtgc cggtgacgcc cggcccggca gtgacgacgg caacgcccgc
      421 ggtgcggcgg acgcggcccc acgcctcggc ggcatgggca gcagactgct cgtggcggac
      481 gtcgaccacg gcaaccccct cgtgccgaca gccctcgtag atcggcatca cgtggccgcc
      541 cgagagcgtg aagatcgtgt cgacgccttc ggcggcgacg gcccgggcga ccaggcgccc
      601 tccctggtga cgctccgaca cagcgcgagc ctactgcctc ggcggcgtag aatgcccgcc
      661 cgtggacctc gacgtcgtgt tcctggggac gtccggctcc acgcccaccg cgcgccggtc
      721 ggctgcggcc acgctcgtgc ggcgcggcgg agagcgcctt ctcgtcgact gcggagaagg
      781 gacgcagagg cagcttctcc gatccgacat cggcctcgtg gagctcgaag aggtgtttct
      841 gacccactac cacgccgatc attacctcgg tctgcccggc atgctgaaga cgttcgccct
      901 acgcggccga gacctcccgt tgacgctcta cgggccctcg ggtctgcgag agctgctgtc
      961 gacgctcgca cgcgtcttcg ggcggctttc cttcccgctc gagaccgtcg agctcgagcc
     1021 gggggacgtc ctcgcccgca gcgagtatcg gatcgaggcc ttcgccgtcc accacgggct
     1081 gcctgctgtg ggctacgcat tcgtcgagga ggagcgcccg ggccgcttcg acgtcgcgca
     1141 ggccgagcgg ctgggtgttc ccgacggccc gctgcgcggg gcgctgcagc gcggggagga
     1201 ggtcacactg gaggacggga cgagcgttcg tcccgagcaa gtcgtcgggg agcggcgccc
     1261 tgggcgaaag ctcgccctcg caggcgacac ggctcctgcc gcctcggtcg tcgaggcttc
     1321 gaggggcgcc gacctgctcg tccacgaggc gacgttctgc gccgaggagc gcgcgagagc
     1381 gcgcgagacg ctgcactcga ccgcgggcga ggcggcgctc gcggcgcgcg aagcgggtgt
     1441 gcagttgctc gcgctgacgc atctctccag ccgctacgca ggacctgacg tcgaggagga
     1501 ggcgcgtcag atctttccgg cttcaatcgt gccgcgcgac ttcgacgtca tacgcatccc
     1561 gttctccgag cgcggccgcc cggagctgac accgaggggt gcacgccacg gccgtgctcg
     1621 ggtagggtca gcgggcgaat gacgaaccag gttcaggttg cggtcgccgg cgatgtgacc
     1681 gaggcggagg agctgcagac catcctgcac gccgccggca tcgaggcgac gctcgagatg
     1741 gctgccgagc acgagcccgg agcgatggac gatccaccgt tgaaggtgct cgtcgcggcg
     1801 aatgacgtcg acgcggcgcg cgacgcgata gaggcgctca ccgagcccga cgagctgatc
     1861 gacgaagact agagtgctcc gcgccggagg tacgccccgc ggaatcttct aggcctcccg
     1921 acttcggcgc ccgcgccggc cgctacgacg agctgcgtcc acaggacgac ggctggtggg
     1981 cgcgcttcga cgcgctcgtg cgcgagggcg acctgaaagg tcgccgggtg ctcgatgtgg
     2041 gttgcggaac cgggctcttg acggcggcac tggccgagac cgggcacgcg aaagtgtggg
     2101 gagtcgagcc cagcgacgag atgctcgccg tggcccgggc acgcgtcccg cgtggcgtcg
     2161 gactgcggcg cggagccgcg gaggagcttt cgttccgcga cgggtggttc gagcgggtgg
     2221 tcttctcgct cgtcgttcac ctcgtcgacc ggcccctcgc cttcgcggaa gccgctcgcg
     2281 tcctctccgc gtccgggaag gtcgctctcg cctcgttcgc gccggagcac ttcgacgtct
     2341 actggctgaa cccatggttt ccgcgaatcg cggagatcga ccgggcgagg tttccgacgc
     2401 ctgccgagct cgagcgtgag ctgcgcgcgt ccggcttcgt cggcgagcgc ttcgtccggc
     2461 tcaccacccg gcaggagatc gaccgtgcca cggcgctgga gcgcatccgc ggacgacata
     2521 tctccacctt cgacctgctg agcgaggacg agctacgcga agggatcgcc cgcgctgagg
     2581 acgagctgcc tgatcgtgtc ccggggcgtc tcgagcagtt gatcgtgatc gcggagcggc
     2641 agccgtgaga tctgtcccca cccccttccc gggtggggga ttcgtcgact agtcggtcgg
     2701 cggcgggatc cattcgacac gcgtcgagcc caccaggccc gggctctcga gcacgtagta
     2761 gcacgagcgg ctcccgtcgg cgtccggcgg cgcggggatg gagcctctcc agaccttttc
     2821 gggctcgatc gtcaccgtcc ggaccgggat cccgttgacg ccgatattga tcggcttgcc
     2881 ttccttcccc agcagggtga gtgcgagggc tccgccgcgc ttacagccgt acacgacgat
     2941 gcccgcctcg ccgagaatgt cgcccacggg tgtgaatcct gcgcgcgacg tcgtcgcccg
     3001 cagcgccccg tccatgcgcc agagcgtctt ctccggctcg tcggtggtca tcgccgcaac
     3061 tgcgagcgcc tcgccttcga gcaccagggt gctcggcgcg actgcacgtg acgccgacag
     3121 cacggtgccg tcctcccgga tcacctcccc ctgctcggac agcgtcaccg agagaacggg
     3181 cagcggcccg tgccactccg cgctcggcag ggcgacgatc cgctggatcg actcgttcca
     3241 gaacaggatg cgcgcgttcg acgtccagag aagctcgccg gtggcgagta ggacggagga
     3301 ctcgtcgccg gcgtcgtcca cccaggtcgt gactgcaggt ccgaacgctc tttcgcgcgc
     3361 cttctcggag agacgggcga cgtccagcgc ggcgacgagc gacgcggcgg ccagcaccgc
     3421 cccggtcgcg agcacggcaa tcgatgcgcc agccgggtat cgatcgtgcc ggcgcgcgac
     3481 gaggacgatc gcgagcacgc cgaacgcggc tgcggcgacc aggacgatcc gagctatcgc
     3541 gtcggacgtg gcgtcggcca gatcgcggag cacaagtgtc tcgaacaggt cgtgcgcgcc
     3601 cgcggcagcg gccgactggt cgatcggggt cagcgccacg agcgcgacca tcgcgatcgc
     3661 cagcaccggc gtgccgatcc gctcccacgg cgcgccgcgt acgccccaga ggcagactcc
     3721 gagcgcgagc agcgggagag cggtgatgag ataccggccg ccgacatggt cgacgttctc
     3781 ggcggcgaag agagcgacct gggctgcgag cagtggaacg aaggaggccg tcgcggcgag
     3841 gaaggctgcg acgggagggt cggtctcgcc cgtgcgggcg gcgcgccaga cgagtgtgcc
     3901 ggtggcgagg aacggcagga aggcgcccag gatcgcgacg taccccgcgt gccagacgag
     3961 ctcccagccc agggccgtcg agtcggggcg cgctccggca gcgtacgcac cgaccacgtc
     4021 gtccagcccc gcatcgtcac tcacgaacac ccaggtgacc gcgagcaggc tgccgagcgc
     4081 gaggatgccg aggatcggcc agagccggcg aaggatcttc gcggagcgga cgaagagcgc
     4141 gtagaggagc gcggcgctga cgagagccgg caggagaagg agagcctgca gccggatcgc
     4201 cgccacgaag gtcaccgcca gcaggaaggc gccctggcgc atcagcgtcg gcgtctcgag
     4261 cacgcgggca agggcggcga gcgccacggc ggtggccgga tagaagagcg cctccgtcat
     4321 gagcagaccc gcgtaggtca gcgccggtgt gagcagcgtc agcgcggcgg ccgcgagtgc
     4381 ccagcgtcgg ccgaggaaac gccgccccca gaggtagacc gggaccgcgg cgagcgagat
     4441 gacgagcgcc tgcagcgcct gagccaggcg gatcccgtcg gcgagctctc ccgccaggag
     4501 cggcagtccc acgaaggccg gatagaggag actgtggtag ggaacgtcga accccctgat
     4561 cgtgagcgtg ccccgttccc agagatcctg gccgagcagt ccgtagacca tctcgtcggg
     4621 ggctatccag ggaacggtga acgtacgcgc cgcgagcagc cgaagccctg cggacacgac
     4681 gaccatcgca ccgagcgcca gcaccgctac gacctcggga cgacgagcag cccatgcacg
     4741 acccccgtcc agcacggtcc gtactgtgcc accagcgccc gggcggcggc ggatttcggt
     4801 gctgtcgcgc ggcgccgcta atgttcgccg cggcctttaa cccggtccag tgaggccgga
     4861 aaggagagag atgacgacac accttccgag ccggtccgca ccggcaacag ttctcgacgc
     4921 ggaggcgatg tcgcggacgc tctcacgcat cgcgcacgag ctgatcgagc ggagcgagag
     4981 cctggatgag atcgccctcg tcggcatcca cacgcgtggc gtgccactgg cgcaacgaat
     5041 ccgccgcctc gtctcggaac gcagcgggat cgacctcgcc ctgggagcgg tcgacatcac
     5101 gttccaccgc gacgacgtct ccgttcgtgc cggcggagcg ccccgccacc cgcagcccgt
     5161 ggttcgtggc accgccctcg acttcccgct ggagggcatg acgtgtgtgc tcgtggacga
     5221 cgttctccat acaggccgga cgattcgcgc cgcgatcgac gccctgctgg agtacggccg
     5281 gcccgagcgc gtcgagctcg ccgtgctcgt cgaccgaggg caccgagagc tgccgatccg
     5341 tccggactac gtggggaaga acctgccgac cgcccggaac gagcgagtgc acgtggagct
     5401 ggtcgaggtg gacgagatcg atcgagtgct cctcgtcgcc gcggaggagg atcgtgatgg
     5461 ctgagataca gctggtttcc cacctgcagc cgccggaccg accgccgcgg cgacaccttc
     5521 tcggggtgtc cgacctgtcg cgcgcggatg tcgagcgcat cctgcgcacc gctcgcgcgc
     5581 tcgagcgctc cctggagcgt gagctgaaga agctgccgac cctgcgcggc cggacagtcg
     5641 tcaatctctt ctacgagtcc tcgacgcgca cgctctcgag cttcgagctc gcggcgaagc
     5701 ggctctccgc ggacacgatg tcggttcgtg ctgcaggctc gtcggtggac aagggcgagt
     5761 cgctcaagga cacggccctt acgttggccg agtacgaccc ggacgtgatc gtggtgcgcc
     5821 acccgcagat cggggcgccg catctcgttg cggcgaccac ccaggcgcac gtcgtgaacg
     5881 gcggcgacgg caagcaccag catccgaccc aggcactgct cgacctctac acgatggaga
     5941 gggcgctcgg gaggctcgaa gggcttcgga tcgcgatcgt cggtgacgtc ctccactcgc
     6001 gggtggcgag atccttgatc gaagcccttg cgctcgtcga ggccgagacg acactcgtcg
     6061 gcccgccgac cctgatgccg cgagggatcg aggcgatggg ttgctcggtc gccagcgaga
     6121 tcgctgcggt cgaggacgcc gacgtgatct acgtcctcag gatgcagcag gagcggatgc
     6181 tgcccggtgc cgcctacgtt ccgtcactgc gtgagtactc ggcgctatgg gcgatcactc
     6241 ccgagcgggt gcgcaagggc caagtcgtga tgcaccccgg gccgatgaac cggggcgtcg
     6301 agatcgatcc gcgcgtggcc gactcagatg ctgcgctgat cacggcgcaa gtccgatcgg
     6361 gtctcgtcgt gcggatggca gtcctgtacg acctgctcac ctcgagcccg gtcgagctgg
     6421 ccaccaggct ggaggttgca tgatgttgag ctgtcggaac gggcgacacg acacgctgac
     6481 catcgtcggc gctcgagtcg tcgacccctg ccaggacgtc gaccagtcac tcgacgtcac
     6541 catcgaggag ggggtgatca ccgagctcca gccgaccgct gcggccgtgc gcgggctcgt
     6601 cctggcgccc gccttcgtcg atccgcacgt tcaccttcgc acccccggcc gcgaggacga
     6661 ggagacgatc gcgagcggga ctgccgcagc cgcggcaggc ggctactgcg caatcctcgc
     6721 gatgccgaac accgatccgg tggtcgactg cgccgccgtg ctcggcgccc tgatcgagcg
     6781 ggcgcgggaa gaggcgatgg tgcccacggg ctttctcgcc tcgatctcga aggggcagcg
     6841 cggggaggag ctcagcgagt tcggcgacct cgctgcttcc ggtgcggtgg gcttcaccga
     6901 cgacggagtc cctgtggcgt cgaccggtct catgcgacgc gcgctgcagt actcggcggt
     6961 ggccgaccga cccctcgcgc tgcacgagga ggagccgagc atctctcgcg acgggcagat
     7021 gcacgagggc gtcgtctcgg ccgagctggg acttgccggc tggccctcgg tcgccgagtc
     7081 ggtgatggtt cagcgggacc ttgcgctcgc cgcgtacgag gagcggcccc tccacgtcat
     7141 gcacgtctcc gcgcgcgagt ccgtcgacgc attgcgtatc gcgcacgccc gcggcgtcca
     7201 ggcgacggca gaggtcacgc cccaccacct cgtgctgacc gacgacgcgg tgcgctcgct
     7261 cgacccgaac gtgaagatga acccgccggt tcggtccgac gacgaccgga cgacgctgat
     7321 cgaggcgttg cgggacggca cgatctcgtg cgtggccacc gaccatgcgc cgcacgcccg
     7381 ccacgagaag gaggcgccgt tcgaagccgc tcccttcggc gtgaccgggc tcgagaccgc
     7441 gttcgccgcc ctctacacgt acctcgtccg acccggggtg gtcccgctgg agacagtgct
     7501 cgagcgcatg tcggcgggcc cggcgcgagc attcggactc cccgtccccc ggatcgcggt
     7561 cggcgagaag gccaatgtcg tcctgctcga cctcgactcg cagtggcggg tgagcgagca
     7621 gagcttccgc tcacgttgcg tcaactcgtg gctgctgggc cacacgctgc acggcgcggt
     7681 cgtgaagacg gtggccgacg gccgggtggt gcacgcggcg tgacggcgtc cgggtacctg
     7741 cttctggagg acgggacggt cttccgtggg cggtctgtgg ccgcgcccgg cgcggtgttc
     7801 ggggaggccg tcttcacgac gggcatgacg gggtaccagg agacggtgac cgaccccagc
     7861 tacgcggagc agctcgtcgc gttcacaacc gcgatggtgt gcaactacgg cgtcgacgat
     7921 gcgcggctcg agtccgatcg ccctcatgcc cgcggcgtgc tgatgcgact ctgcgggggc
     7981 gacgcgtggg cggagtggct cgcggcggcg ggcatggtcg ctctggacga gatcgacacg
     8041 cgggcactga cgctgaggct tcgcgacgac ggtgcgatgc gggccgtcct cgtcgccgac
     8101 gaggccgagc tggcggttgc cgacgccttg cagcaggtgc gggcccagcc gggcatggag
     8161 ggagccgcgc tcgtggcgca ggtttcgacc cgcgagccct acacgccgat tccggagggg
     8221 cgcctccgcg tggccgtcgt cgactacgga gccaagcgtt cgattgtgcg ccgcctggca
     8281 cgggccggcg cggccgtcac ggtcttcccg cacgtgcacg accccgacga gcttgcctcg
     8341 ttcgacgggg tgctgctttc caatggaccg ggcgacccgg agccactgag cgaggagacc
     8401 gaggtggtgc gtgcgctcct cggccgcacg tcggtgctcg ggatctgtct cggccaccag
     8461 ctgctcggct tggccgcggg tcatcgcacg ttcaagctgc ccttcggtca ccggggcgcg
     8521 aatcatccgg ttctcgagcg ccgcagcagt cgcgtgctgg tgacgagtca gaaccacggc
     8581 ttcgcggtcg ccgcgagcga cgacgtagag gcgacctacg tgtcgctcta tgacggcacc
     8641 gtcgagggct tcgatttcgc agagctgcgc gcgcgatcgg tgcagttcca ccctgaggcg
     8701 ggcccgggcc cgcacgacgc ctgg
//
LOCUS       DBNGNK010000175         6755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1821800,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000175 DBNGNK010000000
VERSION     DBNGNK010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1821800"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="AC150V_05010"
     CDS             <1..817
                     /locus_tag="AC150V_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HIR3762316.1"
                     /translation="NAVRTRSSGEGSPPSRVLFSALSRCPSCIFRHKVIGSLIDTCTE
                     GKIADMAHQWLFKSETYDNCNCACNCGCQFNLPSTHGYCQSAFVGNIVEGHFDDTSLA
                     GLKWAGLYKWPGEIADGNGKRQIIIDHRADEAQRVALETIISGEACEPLSNMFSVFAF
                     TCSEFCETLFLPIDLETNLELRTARVDIPGVMTSRGEPIINEFNDQPFHIALARPSGS
                     FEFTYAEIGQGTTTVTGEMAMAFEDSWAHFCVHHFNQDGLVRERSRLTAWLDA"
     gene            894..2324
                     /locus_tag="AC150V_05015"
     CDS             894..2324
                     /locus_tag="AC150V_05015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3762317.1"
                     /translation="MTLPTRLVSACAIVVDMNTITSHTSALAAELRGGGFVGRLVEPT
                     DRAYDIARRSWNGALDARPAAVAYASDADDVAAALTAARAAGLGFSIRGGGHSVSGRS
                     TRDAALCIDLRALNAVDVDPERMIVRVGGGALLYELDAATQEHGLAVPAGQVSHTGVG
                     GLTLGGGMGWLMRHHGLTIDSLRAAEAVLADGRRVLASADDHPDLYWALRGGGGDFAV
                     VTRFEFQAHRVGPLVLAGMLVYPWGRAHEAVRASRELMLGAPDALTSFVVLITAPPTA
                     PFPPELQGRPVVVVGVAWSGDLARGERVLAPLRSGNPPALDLVAPMPYVALQTMLDET
                     APHGWQFYDRLHYLDEVGDEYIDVLLAGFERVPSPQSHVITTWLGGAIDRVPPGATAF
                     GHRSARALTWFIGCSGNEPVGEAADWVRSLWDDTRRFGTGGVYVNALNAERSVRDAYA
                     DDVWQRLVAVKRRYDPDGAFDGNGIR"
     gene            complement(2321..3937)
                     /locus_tag="AC150V_05020"
     CDS             complement(2321..3937)
                     /locus_tag="AC150V_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3762318.1"
                     /translation="METKLAGWKHFAGADWAAARDAFLAALEVEPGDPEALDGLGQSL
                     WWLGRRDEAIDLRRDAYATYRERGDNRQAGLLAVYLAGEHRIDGQRAAAAGWLARARR
                     LLAHEGAVPERGWLAIEDAKRSDDPVTAEEHARAALDLAQVVGDPNVECMALAQLGRA
                     LVGQGRVEEGLTLLDEAMTVALGGESRDPLAVGDACCTTLVVCDRLCDLDRAAQWCEL
                     VVDFTERRRYTPVQSWCRGVYGGVLVRAGEWDRAEEVLAEALRRNRVRRASDIALPLA
                     VLAELRLRQGRVDEAATLLVDHEDDRAALAPLVQLHLQRGETTLAAAVLDRAFARAND
                     DPDALVLRGAVAVAQCDLAAAESIAEQLRGLAEQLARDDLAAEAALLAGRASAARGDA
                     AAAESDFEDAVALFASLRFPLEEARARLALARVQADTESPLARRSGQAALDVFERLGA
                     RRDADEAAALLRSLGTAGRTARRGERGQLTAREREVLVLLAAGLTNAEIAERLVIAPK
                     TAEHHVGHVLAKLGVRSRAEAAAHAVREGL"
     gene            complement(4028..4579)
                     /locus_tag="AC150V_05025"
     CDS             complement(4028..4579)
                     /locus_tag="AC150V_05025"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR3762319.1"
                     /translation="MSDRSHPAGAVAGAVRVFIASSLDGFIAGPDDDLSWLPEGSGED
                     YGYAAFMADTAAILMGSSTYRVVEGFGGPWPYGETPVFVATTRPLSPAVGTVHPVRGT
                     PHELLEEIRERTDGNVYLDGGDLIRQFLDAALVDELTITLIPVVLGAGSPLFAGTTQR
                     HALELLSTRAYPGGIVQLTYATG"
     gene            complement(4591..5169)
                     /locus_tag="AC150V_05030"
     CDS             complement(4591..5169)
                     /locus_tag="AC150V_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3762320.1"
                     /translation="MSKRAAATEATRRRIVEATLELHDEQGIRATSWDEIAAEAGVGV
                     GTVYRHFPSLAELVPACGVLTFERLALPDRDEVAALFRGRRSRRARLRRLVDLLFGLY
                     GRGAGAIENVRRERDDPDLPMLEDAHERIESALEGLRTEALRPFAVSDEQRRLVRAFT
                     DIPVWRSLGEQGICGERAVTVVEAALNGALDC"
     gene            5274..5750
                     /locus_tag="AC150V_05035"
     CDS             5274..5750
                     /locus_tag="AC150V_05035"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008127 - quercetin 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HIR3762321.1"
                     /translation="MSTTMTSMEPIAFAAGEGDRRWWFGELATVKLTAAQTGGAFSLV
                     EVIAPAGLDVPLHVHHGEDETFVILRGAVSFEVGGQTVEAGPGTVLFAPRDVPHRYTV
                     GNEEAAMLFLFSPGGFEGFVYESSVPAAAAELPPADVRPPADVVSIAAAYRAEILG"
     gene            complement(5946..6161)
                     /locus_tag="AC150V_05040"
     CDS             complement(5946..6161)
                     /locus_tag="AC150V_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3762322.1"
                     /translation="MTSRELEVLRHVAAGDTNCAVAARLWVSDQTVKFHLSNIYRKLD
                     VSGRHEASQWASRNGLLDERGSDALSS"
BASE COUNT         1025 a         2345 c         2309 g         1076 t
ORIGIN      
        1 caacgccgta agaacgcgct cgagcggcga gggttcgcca ccctccaggg tgctcttttc
       61 cgcgctctct cgctgccctt cttgcatctt ccggcacaaa gtcatagggt cgttgatcga
      121 tacctgcacc gaggggaaga tcgccgacat ggctcaccaa tggctattca agagcgaaac
      181 gtacgacaac tgcaactgcg catgcaactg tggttgtcaa ttcaacttgc cgagcaccca
      241 tggatattgc caatcggcct tcgtcggcaa tatcgtcgaa ggtcattttg acgatacgtc
      301 gctcgccggg ttgaagtggg cggggctgta caaatggcct ggcgaaatcg cagatgggaa
      361 cggcaaacgt cagatcatta ttgaccatcg tgccgatgaa gctcaacggg tcgcgcttga
      421 aaccatcatt tcaggtgaag catgcgagcc actgagcaat atgttttccg tcttcgcgtt
      481 cacatgctcg gaattctgcg agaccttgtt tctgcccatt gatctcgaga cgaacttgga
      541 gctcagaacg gcaagagtcg atatacccgg cgtcatgacg agccgcggcg agccgatcat
      601 caacgagttc aatgaccaac cgttccacat cgcgctggca cgccctagtg gcagtttcga
      661 gttcacctac gcagaaatcg ggcaaggcac caccacggtc accggtgaga tggcgatggc
      721 gttcgaagat tcatgggccc atttctgtgt tcaccacttc aatcaggatg gactggtgcg
      781 cgaaagatcg aggctcacag catggcttga cgcatgagcc agcactctgt gtgggggaga
      841 ggttgactcg actgcccggg cggtgctcac gggaagatag gggtacgagt tgggtgacac
      901 tcccgacgcg gcttgtctct gcctgcgcga tcgtggtcga catgaacacg atcacctcac
      961 acacatccgc actcgccgcc gagcttcgtg gcggcggctt cgtcggtcgc ctcgtcgagc
     1021 cgaccgatcg agcctacgac atcgcgcggc gcagctggaa cggcgcactc gacgcccggc
     1081 cggccgcggt tgcctacgcg tccgacgccg acgacgtggc cgcggcgctg acggctgccc
     1141 gcgccgccgg cctcggcttc agcatccgcg ggggcggcca ctcggtctcc ggtcgctcga
     1201 cccgtgacgc ggcgctctgc atcgacctga gggctctgaa cgccgtcgac gtcgatcccg
     1261 aacggatgat cgtccgcgtc ggcggcggcg cgttgctcta cgagcttgat gccgccactc
     1321 aggagcacgg cctggcggtt ccggccggcc aggtctccca caccggcgtc ggcggtctca
     1381 ccctcggcgg cgggatgggg tggctgatgc gtcaccacgg cctgacgatc gactcgctcc
     1441 gtgccgctga ggccgtcctc gccgacggtc ggcgcgtgct tgcgagcgcc gacgaccacc
     1501 cagacctcta ctgggcgctc cgtggcggtg gcggtgactt cgcggtcgtc acccgcttcg
     1561 agttccaagc tcatcgtgtc gggcccctcg tcctggcggg gatgctcgtg tacccgtggg
     1621 ggcgagcgca cgaggcagtg cgcgcaagcc gcgagctgat gctgggcgcg ccggacgcgt
     1681 tgacgagctt cgtcgtcctg atcaccgcgc cgccgacggc gcccttccca ccggagctgc
     1741 agggccgacc cgtggtcgtc gtcggggtgg cgtggagcgg cgacctcgcc cgaggcgagc
     1801 gcgtgctcgc cccgctgcgt tctgggaatc cgccggctct cgatctggtc gcgcccatgc
     1861 cgtatgtcgc gctgcagacg atgctcgacg agacggcccc gcatggctgg cagttctacg
     1921 atcgactgca ctacctggac gaggtgggcg acgagtacat cgacgtgctc cttgccggct
     1981 tcgagcgcgt cccgtcgccg cagtcccatg tgatcacgac ctggctcggc ggggcgatcg
     2041 accgcgtccc ccctggcgcc acagcgttcg gtcaccgcag cgcgcgtgcg ctcacctggt
     2101 tcataggctg ctcgggcaac gagcccgtcg gagaggcagc cgactgggtg cggagcctgt
     2161 gggacgacac ccggcgcttt ggcaccggcg gtgtctacgt caacgccctc aacgccgagc
     2221 ggtctgtgcg cgacgcgtac gccgacgacg tctggcagcg tctcgtcgcg gtcaaacgcc
     2281 gctacgaccc ggacggcgcg ttcgacggca acggcatccg ctagagccct tcgcgaacgg
     2341 cgtgcgcagc tgcctcggcg cgactgcgca cgcccagctt ggcgagcaca tgaccgacgt
     2401 ggtgctccgc cgtcttgggc gcgatgacga ggcgttccgc gatctccgcg ttcgtcagcc
     2461 cggccgccag cagcaccagc acctcgcgct cccgcgcggt cagctggccg cgctcgcccc
     2521 gacgcgcagt tcggcccgcc gtgccgaggc tgcgaaggag cgctgcagct tcgtcggcgt
     2581 ctcggcgggc gcccagccgc tcgaagacgt cgagtgccgc ctggccagac cgccgtgcca
     2641 ggggcgactc ggtgtcggcc tggacgcggg cgagtgcaag gcgtgcgcgc gcctcctcga
     2701 gagggaagcg aagggatgcg aagagggcga cggcatcctc gaagtcgctc tcggctgccg
     2761 ccgcgtcgcc ccgagccgcc gacgcgcggc ctgcgagcag tgccgcctcg gccgccagat
     2821 cgtcgcgtgc gagctgctcc gccagtccgc gcagctgctc ggcgatcgac tcggccgccg
     2881 cgagatcaca ttgggcgacg gcgacggcgc cgcgcagcac gagcgcgtcg ggatcgtcgt
     2941 tcgcgcgcgc gaaggcgcga tcgagcacgg ccgcggcgag cgtcgtctcg cctcgctgga
     3001 ggtggagctg tacgagtgga gcgagcgcgg cacggtcgtc ctcgtggtcg acgagcagcg
     3061 tcgccgcctc gtcgacgcgg ccctgacgca ggcgcagctc cgcgagcacc gccagcggca
     3121 gcgcgatgtc gctcgcacgg cgaacccgat ttcggcggag ggcctccgcg agcacctcct
     3181 cggcgcgatc ccattctccg gcccgtacga gaacgccgcc gtagacgccg cggcaccacg
     3241 actgaaccgg cgtgtagcgt cgtcgctccg tgaagtccac gaccagctcg caccattgcg
     3301 cggcgcggtc caggtcgcac aggcggtcgc agacgacgag cgtggtgcag cacgcgtcgc
     3361 cgacggccag cggatcgcgg gactcaccgc cgagcgcgac cgtcatggct tcgtcgagca
     3421 gggtcaaccc ttcctcgacg cggccctgtc cgacgagcgc acggccgagt tgcgcgaggg
     3481 ccatgcactc cacgttcggg tcgcccacga cctgcgcgag gtcgagtgcg gcgcgcgcgt
     3541 gctcctctgc cgtgaccggg tcgtcgctcc gcttcgcgtc ctcgatcgcc agccagccgc
     3601 gctctggcac ggcaccctcg tgagcgagaa gccgtcgagc acgtgcgagc cagccggcgg
     3661 cggccgcccg ctgcccgtcg atgcgatgct cgcctgcgag gtagacggcc aggagtccgg
     3721 cctggcggtt gtcgcctcgc tctcggtacg tggcgtaggc gtcgcggcgg aggtcgatcg
     3781 cctcgtctcg cctgcccagc caccagagcg actggccgag cccgtcgagc gcctccggat
     3841 cgccgggctc cacttcgagc gccgcaagga aagcgtcgcg cgcggccgcc caatccgcgc
     3901 cggcaaagtg cttccagccg gcgagcttcg tctccatggc gggccgaaga gtatccgcgt
     3961 tcgagcacga tgggcccgca gggacagaca agtgctccct ggcccctgcg cagccctacg
     4021 tcggcctcta gcccgtcgcg taggtcagct ggacgatgcc gcccggatag gcgcgcgtcg
     4081 acaggagctc gagcgcatgc cgttgtgtcg tgccggcgaa gagcggcgag ccggcgccga
     4141 gcacgacagg aatgagcgtg atcgtcaact cgtcgacaag ggcggcgtcg agaaactgtc
     4201 ggatcaggtc gccaccgtcg aggtagacgt tgccgtccgt ccgctcgcga atctcttcga
     4261 gcagctcgtg gggcgttccg cgaaccggat gcaccgtgcc gacggcgggc gacagggggc
     4321 gcgtcgtggc gacgaagacc ggggtctcgc cgtacggcca gggcccgccg aacccctcca
     4381 cgacgcggta cgtcgaactg cccatcagga tcgccgccgt gtcggccatg aacgcggcgt
     4441 agccgtagtc ctcgcccgag ccctccggca gccaggacag atcgtcgtcg gggccggcga
     4501 tgaagccgtc gagcgaggat gcgatgaaca cgcgtacggc tccggccacg gcccctgcag
     4561 gatgggagcg atcgctcaca gacagtcctt ctagcagtcg agggcgccgt tgagagccgc
     4621 ctcgacgacc gtgacggccc gctctccgca gatcccctgc tcgccaagcg agcgccagac
     4681 cgggatgtcg gtgaacgcgc gtacgaggcg tcgctgctcg tcggagaccg cgaacggccg
     4741 gagcgcctcc gtgcgcaggc cctcgagcgc gctctcgatc cgctcgtgag catcctcgag
     4801 catcggcagg tcggggtcgt ctcgctcccg gcggacgttc tcgatcgcgc cggcaccccg
     4861 cccgtagagc ccgaagagga gatcgacgag gcggcgcaag cgagcccggc gggaacggcg
     4921 tccgcggaag agcgcggcta cctcgtcccg gtcgggcaag gcgagtcgct cgaacgtgag
     4981 gacgccgcac gcggggacga gctcggccag ggacggaaag tgccggtaga cagtgcccac
     5041 cccgacgccc gcctctgctg cgatctcgtc ccagctcgtc gccctgattc cctgctcgtc
     5101 gtgcagctcg agagtcgcct cgacgatccg gcgccgagtc gcctcggtgg ccgcggctct
     5161 tttcgacata ttgtattttc gcggagacat ttgttgatat aatcttctcc gtcaattagt
     5221 atactcctcg taaggtcatc tgaaagtccg atctggagac taaggagaag aagatgagca
     5281 cgaccatgac atcgatggag ccgattgcgt tcgccgccgg cgagggtgac cgccgctggt
     5341 ggttcggcga gctcgccacg gtgaagctga cggccgcaca gaccggcggc gcgttctcgc
     5401 tggtcgaggt gatagcaccc gccggactcg acgtgccgct gcacgtgcac cacggcgagg
     5461 acgagacgtt cgtcatcctc cgcggcgccg tcagcttcga ggtcggcggg cagacggtcg
     5521 aggcggggcc gggaacggtg ctcttcgcac cccgcgacgt gccacaccgc tataccgtcg
     5581 gcaacgagga ggcggcgatg ctgttcctct tcagcccggg agggttcgag ggcttcgtct
     5641 acgagtccag cgtcccggcc gcggcggcgg agctgcctcc cgccgacgtg cgaccacctg
     5701 ccgacgtcgt ctccatcgca gcggcctacc gcgccgagat tctcgggtga ggtgacgcgc
     5761 tcgtagcgca gaccgtacgg gcggcggcag gttgccgtcg agcgtcatat cgcgctcgat
     5821 cgggcccctt cagcgtcgag actcgacgcc gccgccgccc tgcgcggtcc gcaggagcgc
     5881 ccgcggcgaa tctccacggc tacggcgagt agacgcgttt cgaccccgcc gccgtgccgg
     5941 ccggatcagc tggacaaagc gtccgagccc ctctcgtcga gcagtccgtt ccgcgacgcc
     6001 cactgtgagg cctcgtggcg gccgctgacg tcgagcttgc gatagatgtt cgagaggtgg
     6061 aacttgaccg tttgatcgct cacccacagg cgtgccgcga ccgcgcagtt cgtgtcgccc
     6121 gcggcgacgt gccgcagcac ctcgagttcg cgactggtca acccgccggg cctcgcgcgt
     6181 cgcgccgttc gctcggctcg ggcggatacg aaagccgtcc gtgagggtgc gtctgcgggt
     6241 ggcgtcctca tgggctgccc gctgaaccgg ataggaagaa gagcatctcg agcatggatc
     6301 tctccgcgct tcgacgatcg tatgtcggat cctgcacgcc cgaggccgct cgcggcccga
     6361 atgcccgcgc tggcccgtcg acacgccgcc ggccggtgct ccgcaccgcg ctcgagctgt
     6421 cgctctcggt cgggcgaggg gcgtcctgct tgtctcgtgg gtgattgccg gtttacgatc
     6481 gacccgcagg cgactcagtt ccccctgcgg tcggccgtcc cggacggcgc cggtgtcctc
     6541 ttcccgcggg actaccagtt cgtgcgcctc cgggacggcg gcagaagagc cggacgcgtg
     6601 cgcccaagcg cggcaccgtt ctcatggcga agaagggacg tcagcctcgt cagggcgcct
     6661 gacggctccg ctctcgacga cgagttcgaa gagagcgcag ttcccggccg gccccggcca
     6721 ggcacggcgg tgggcgacga tgtcgaggcc gtccg
//
LOCUS       DBNGNK010000176        12732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_674263,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000176 DBNGNK010000000
VERSION     DBNGNK010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 12732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12732
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_674263"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /locus_tag="AC150V_05045"
     CDS             complement(<1..467)
                     /locus_tag="AC150V_05045"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3762323.1"
                     /translation="MASEHQIRVGEVAIGGGAPVVIQSMTLTKTHDVEATTAQIAELA
                     SAGCEVVRVAVPKNEDAAALPLIIRLSPLPVIADIHFNASLALKAIEAGVAAVRINPG
                     NIGGPDKVEQVVRAAKRAEVPMRIGANSGSLPEHLTDLAREDQAEALVAAAIE"
     gene            complement(511..1719)
                     /locus_tag="AC150V_05050"
     CDS             complement(511..1719)
                     /locus_tag="AC150V_05050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR3762324.1"
                     /translation="MSVVVAILGLGLLIFVHECGHFFASLALRMRPRRFYVGFPPAIA
                     KVKRNGIEYGLGAIPLGGFVKIPGMHRPAPSDVDHQFARVLEEAPDLAGPFDRLRRSL
                     STGDMEAAREAMPALRKQVEERHLSPKTRTAAEKGMTELDDGLGPDAYWRAPTWKRIT
                     VIFAGPAANIVLALSLFAVLFTTSGGRATTTVDTVADGSPALAAGLQPGDEIFSIDGE
                     VVGATDISRTISASKGKPLTLMVVRGSNVIALGPVAAKEADGVYRLGFVLGGEGLGPV
                     SAVGESFRVTGLVTKEIGAAIGRLVTGEGREEIASPVGIVQGSSQAAKQGAEQYLWVL
                     ALISLSIALLNLLPLLPLDGGHIAFAIVEGIRGRSVGREIYERVSVVGIGLVLLLFFI
                     GLSNDIGRLS"
     gene            complement(1716..2792)
                     /gene="dxr"
                     /locus_tag="AC150V_05055"
     CDS             complement(1716..2792)
                     /gene="dxr"
                     /locus_tag="AC150V_05055"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR3762325.1"
                     /translation="MRRVALLGATGSIGRQTIGIVEAHPELELCALMSGSQPLDGLAE
                     TQGVEHTQIGGDPVRVLEESRPDIVLNAIVGFAGVRATLWSLEAGIPRALANKESLVA
                     AGELARSAQERGGGPLLPVDSEHSALFQCLEGRDLASVHSLVLTASGGPFRGRKRDEL
                     ADVTAEQALAHPTWRMGRKISVDSATLANKGLELLEAHVLFDLPFEQIEVTVHPTSVV
                     HGLVRFHDGAALAHVGYPDMRVPISFALCYPDRAATPVPPIDLADGLTLEFEPPDVEA
                     FPLLQLAREAGEAGGTYPCVYNAANEVGVAAFLDGRIGFLEIADTVESALSACDGSPA
                     RDLDELVAVDATARKLAERRLAPA"
     gene            complement(2819..3196)
                     /locus_tag="AC150V_05060"
     CDS             complement(2819..3196)
                     /locus_tag="AC150V_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762326.1"
                     /translation="MHSTRRPLLLLLVLVALLGVAAGCGGSSEGESSSGAATDTSGSD
                     SAGSSPAPSGSGDVDTSSPSYQGGYELCQEGPLEYVAGLFGAEPTREAVARAVAEQVG
                     GAAGAEDFAAGRQGCLDAFATNR"
     gene            complement(3243..4040)
                     /locus_tag="AC150V_05065"
     CDS             complement(3243..4040)
                     /locus_tag="AC150V_05065"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR3762327.1"
                     /translation="MSAFWSRILVAIALLPLVLGLVYVGGWWLWGLALAGSLIALHEY
                     YRITKSLRPLVLGGYVALVLALLGTQLGGAPWLLAGIAATFPLAFFLYGISDARPSFN
                     TAVGTTLLGVAWVGIGIGLLLLVRDIPEHGRLAIFTVLLAVFADDTFAYFVGRLIGRH
                     KMAPAISPGKSWEGFAGGTVAAVAVAFFAMYEQGYLEIWESIVLGVAISVAAVTGDLF
                     ESAVKRDMAVKDSGTLLAGHGGVLDRVDSILFAGPAAYVVLLAFGKA"
     gene            complement(4044..4712)
                     /gene="uppS"
                     /locus_tag="AC150V_05070"
     CDS             complement(4044..4712)
                     /gene="uppS"
                     /locus_tag="AC150V_05070"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008262574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR3762328.1"
                     /translation="MAIIMDGNRRWAENRGLPVAEGHRAGTRALRRTVEAAIDLGVKS
                     LCVYAFSTENWTRPADEIESLMEILGETIERELPDLQRQGVRTRFIGRRDRAPENLRR
                     RMERLEEATATNDRLLLWIAFDYGGRAELVEAARRLVDDALLPDEIDEAAYAARLDAP
                     DVPDIDLLVRTSGEQRISNFLLWHAAYAELVFTDTLWPDFDEVDLRHALDEFARRQRR
                     FGGR"
     gene            complement(4802..5365)
                     /gene="frr"
                     /locus_tag="AC150V_05075"
     CDS             complement(4802..5365)
                     /gene="frr"
                     /locus_tag="AC150V_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015863476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR3762329.1"
                     /translation="MGAVEDFIADAERRMDKSIEATRNEFNTVRTGRASAALLDRITV
                     DYYGTPTPLKNMATISVPEPRLLTVQPYDPTQVKAIEKAIQESDLGLTPSNDGKVIRL
                     PIPQLTEERRKELVKVVRRYAEDGKVAIRNVRRDVMHHLKDLVANGEVGDDEERRGET
                     QVQKMTDDHTGRIDELLKHKEDEIMEV"
     gene            complement(5366..6163)
                     /gene="pyrH"
                     /locus_tag="AC150V_05080"
     CDS             complement(5366..6163)
                     /gene="pyrH"
                     /locus_tag="AC150V_05080"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR3762330.1"
                     /translation="MSARSETAEHPATEVAGKPFSRILLKLSGESLMGERDYGVDPET
                     VNAIARELVGVQGAGTELAVVVGGGNFYRGLAEAADSGMDRATADYGGMLATLLNALA
                     LQDALERLGADTRVQSAIDVSEVAEPYIRRRAIRHLEKERIVIFAAGTGNPFFTTDTA
                     AALRALEIGAEALLMAKYGVEGVHDGDPRSDPSARFLPRLTHLEAIERGLRVMDTTAL
                     SLCMENGLPIFVFGLQDGNIKKVAGGERVGTIISTETLGVSTPGKEV"
     gene            complement(6160..6966)
                     /gene="tsf"
                     /locus_tag="AC150V_05085"
     CDS             complement(6160..6966)
                     /gene="tsf"
                     /locus_tag="AC150V_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR3762331.1"
                     /translation="MTEISASLVKELRDMTGAGMMDCKRALQESGGDLEDARRILREK
                     GIASAAKRAGRATTEGIVETSVDNGVGVIVGVGCETEPVSKNDEFREFVSRVVGAVLD
                     GGPTATEGLDGERTELSARLGENIVVVGAERFDADGGVLASYVHPPANKIGVLVRLEG
                     GSAELARQLAMHISFAAPEWVTGEDVPDEVVAAERQIYLSSDEVQSKPEGAREKIVDG
                     MLAKRFLAASPGGVLLEQAWIHDASKTVGQALDEAGATASGFSRVSVAGT"
     gene            complement(6963..7868)
                     /gene="rpsB"
                     /locus_tag="AC150V_05090"
     CDS             complement(6963..7868)
                     /gene="rpsB"
                     /locus_tag="AC150V_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR3762332.1"
                     /translation="MAVVSMKELLEAGVHFGHQTRRWNPKMRRYIFTERGGIYIIDLQ
                     QTQTLVQTAYDFVRNLAERGGTVLFVGTKKQAQDAIVEEASRVGMPYVNNRWLGGLLT
                     NWRTISDRISYLHDLRRLKEDDQLDLLPAKERISLERELEKLEANLGGVADMRKQPDA
                     VFIVDLRKEQLAVNEARRLGLPVIALVDTNCDPDEVDFVIPGNDDALRSCGLIIRAIA
                     DGIAAGATKVTVEELETPTPSPAERTPEAAGDSAAGAAPESEQAAAPEVEEAVEPAAA
                     ADEAQPAQPAEEGQAVEVAEGSEKA"
     gene            complement(8002..8898)
                     /locus_tag="AC150V_05095"
     CDS             complement(8002..8898)
                     /locus_tag="AC150V_05095"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR3762333.1"
                     /translation="MAELRFRVPDRDAGKRLDLVLAGRPEVGARSVAERLIGSGQVTV
                     DGAQRAKSHRLAGGEQVEAQLPVTTVLDEEALDVAVAYSDEHLLVVDKPAGLVVHPAG
                     RARSGTLVHGLVGLGAKGGEEGRPGIVHRLDRDTSGLMIVARSEDAHRRLTGLMRRRA
                     IDRRYLALVRGAPRSRTGRIEAPIGRDRVDRRRHSIDTHTPRAAVTWFELRELLPAHA
                     LLEVRLETGRTHQIRVHLEAIELPVAGDPVYGVKGDLGLARQFLHAHRLRLTHPFTGE
                     KLDVRSPLPPDLESALARAHEP"
     gene            complement(8870..9469)
                     /gene="lspA"
                     /locus_tag="AC150V_05100"
     CDS             complement(8870..9469)
                     /gene="lspA"
                     /locus_tag="AC150V_05100"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR3762334.1"
                     /translation="MSEPLGQRSVERRRLDVRVGSTTNALEPISSAERSLRAGPVQWL
                     SLLLVAGAVVGADQLTKRVVASALGLGKSVDILGPFSIHHVRNSGIAFGLFSSATAIV
                     IVLTTLAVGSMVVFFARSGQRHPMLPVALGFVLGGSLSNLVDRLRFGYVTDFLDFDHW
                     PAFNLADTFIVAGVGLLFVSFVVADRTSARVGRAPLSRS"
     gene            complement(9466..9822)
                     /locus_tag="AC150V_05105"
     CDS             complement(9466..9822)
                     /locus_tag="AC150V_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR3762335.1"
                     /translation="MTLDLKARKAELLDLRDRLTAAAEYVEHADDNAGELSSPSGDQH
                     MADHASDIFDRELDETLEENAEHVLQEIDDALARIAAGTYGLCSVCGEPIPEERLEAV
                     PYATLCIEDKRKQERG"
     gene            complement(9819..10112)
                     /locus_tag="AC150V_05110"
     CDS             complement(9819..10112)
                     /locus_tag="AC150V_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR3762336.1"
                     /translation="MDASTARLRLRVSPGAARSKVVGRYGQAWKVRVAAAPERGRAND
                     AVVDLLAARLRVARPDVRVVSGAGARDKVVEVAGMSLEEAERRLEGADGKENG"
     gene            complement(10131..10721)
                     /locus_tag="AC150V_05115"
     CDS             complement(10131..10721)
                     /locus_tag="AC150V_05115"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR3762337.1"
                     /translation="MPLTPVEIRHQELKRTLFGYKRAAVDRLMTEVADSFELVWRERA
                     ELADRVEVLDAEVKRHLELEGLLRQTLVSAERAAEEVKERARKEADLIVNEAHSEARS
                     IKRDALAERQRLLAEAQRVQAILRSALEVVDEARDAAPAEPLGTDAPAEAQRPASPLP
                     PASESGTPTAAAGGSDDDRREGPGDETDPDVRRLAG"
     gene            complement(10740..11060)
                     /locus_tag="AC150V_05120"
     CDS             complement(10740..11060)
                     /locus_tag="AC150V_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR3762338.1"
                     /translation="MTMGGCLAVLGLLADAASSIATFVEVFIYVYILLIFAYIITSWI
                     RLPYSPTLSRIQRFLFDVCDPYLRLFRRVLPPLGPLDLSPIVGIFVLVIVQRFLVALI
                     ERVL"
     gene            complement(11121..11660)
                     /locus_tag="AC150V_05125"
     CDS             complement(11121..11660)
                     /locus_tag="AC150V_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR3762339.1"
                     /translation="MGFGEVWSRTLVYFGIAEDEEWDEDGYATEEELGRDYRSRERAN
                     VRRLPRRQEPTEWADTDMDSEPSAVATRTRRTRLRGVDQGGGQVRVLLVLPRSFNDAQ
                     QIADRFKDQVPVILNLQSADNELSKRLIDFASGLTYALDGSMQRVAEKVFLLTPRNVE
                     VSAEERARMLDRGGFFNQA"
     gene            complement(11705..12298)
                     /locus_tag="AC150V_05130"
     CDS             complement(11705..12298)
                     /locus_tag="AC150V_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate enzyme"
                     /protein_id="HIR3762340.1"
                     /translation="MTPAGVRDAYDEIREATGPDVTVVAATKYVALEDMAMLAEAGVE
                     VVGENRAQDLERKHAVYGSAFRWHFIGHLQSNKVKVVNRLCELVHSLSSESAARRLEI
                     PALLEVNLSGEPSKSGVPPADVPRYLDEYPLIRGLMTMPPLSDDPESSRPFFERLRRL
                     AAEYALPELSIGTSQDWRVAVEEGATLIRVGSVLLRG"
     gene            complement(12295..>12732)
                     /locus_tag="AC150V_05135"
     CDS             complement(12295..>12732)
                     /locus_tag="AC150V_05135"
                     /inference="COORDINATES: protein motif:HMM:NF025112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762341.1"
                     /translation="RAGTILERLRYPVRLQRRVTAIVAAHAFSPRAEIDPARARRFLA
                     AHGDEIAFELVALKEADLAAKGVPTEEYGILQRFRALLEHEVASPHRLADLAVDGDDL
                     LELGLAEGPAVGELLRRLLDQVLVDPSQNEKDALLALARKELA"
BASE COUNT         2061 a         4416 c         4188 g         2067 t
ORIGIN      
        1 tcttcgatcg ccgcggcaac gagagcctcg gcctggtctt cacgggcgag gtcggtcagg
       61 tgctcgggga gcgatccaga gttggctccg atccgcatcg gtacctctgc gcgcttcgcc
      121 gcgcgcacga cctgctcgac cttgtcgggg ccgccgatgt tgccggggtt gatccgcacg
      181 gctgcgactc ctgcctcgat cgccttgagc gccagcgagg cgttgaaatg gatgtcggcg
      241 atgacgggca acggcgagag ccggatgatc aggggcagcg cggcggcgtc ctcgttctta
      301 ggcacggcca cccggacgac ttcgcagccg gccgaggcga gctccgcgat ctgcgcagtc
      361 gtcgcctcga cgtcgtgggt cttggtcagc gtcatcgact ggatgacgac cggcgcaccg
      421 ccgccgatcg cgacctcccc cacccgaatc tgatgctcgc ttgccaccgt cggtcagtgt
      481 aaaggcggcg atacgacgtg cacagaccgt ctagctcagc cgaccgatgt cgttggagag
      541 cccgatgaag aagagcagga ggacgaggcc aatgccgacc accgagacgc gctcgtagat
      601 ctcgcgcccc accgaccgtc cacgaattcc ctcgacgata gcgaaggcga tgtggccgcc
      661 gtcgagcggt aggagcggca gcaggttcag caaggcgatc gacagcgaga tcagcgcaag
      721 tacccagagg tactgctcgg ctccctgttt cgccgcctgc gacgagccct gaacgatccc
      781 gaccgggctc gcaatctcct cccgtccctc tcctgtgacg agtcggccga tcgccgcccc
      841 gatctccttc gtcacgaggc ctgtgacacg gaacgactcg cccaccgccg aaaccgggcc
      901 caggccctcg cctccgagga cgaaaccgag ccggtagacg ccgtccgcct ccttcgcggc
      961 caccggaccg agcgcgatca cgttactgcc gcgcacgacc atcagcgtga gcggcttgcc
     1021 cttcgacgcc gagatcgtcc gggagatgtc cgtcgcccca accacctcgc cgtcgatcga
     1081 gaagatctcg tcacccggct gtaggccggc agcgagcgcc ggtgacccgt cggccaccgt
     1141 atcgaccgtg gtggtggcac gcccgccgga tgtggtgaag agaacggcga agagagaaag
     1201 cgcgagcacg atgttcgccg ccgggccggc gaagatcacc gtgattcgct tccaggtggg
     1261 cgcccgccag tacgcgtcgg gcccgagccc gtcgtcgagc tccgtcatgc ctttctccgc
     1321 ggccgtacga gtcttgggcg agaggtgccg ctcttccacc tgctttcgga gcgcaggcat
     1381 cgcctcacgc gccgcctcca tgtcgcccgt cgagagcgag cgacggaggc gatcgaacgg
     1441 gccggcgaga tcgggcgctt cctccaggac gcgcgcgaac tgatgatcga cgtccgacgg
     1501 cgcaggccgg tgcatgccgg ggatcttcac gaagccgccc agtgggatcg cgccgagccc
     1561 gtactcgatc ccgttccgct tgaccttcgc gatcgcgggt gggaaaccga cgtagaagcg
     1621 gcgtggtcgc atccgtagcg ccagggacgc aaagaagtga ccgcactcgt ggacgaagat
     1681 gaggagaccg agcccgagaa tggcgacgac gacgctcatg ccggggcgag cctccgctcc
     1741 gccagcttcc gcgccgtggc atcgacggcg accagctcgt cgaggtcgcg ggccggcgag
     1801 ccgtcgcacg ccgacagcgc gctctcgacc gtgtcggcga tctcgagaaa gccgatcctc
     1861 ccgtcgagga aggcggccac acctacctcg ttcgccgcgt tgtatacaca cgggtacgtc
     1921 ccccccgcct cgccggcttc gcgtgcgagc tgcagcaacg ggaacgcttc gacgtcgggc
     1981 ggctcgaact cgagcgtcaa gccgtcggcg agatcgatcg gaggcacggg tgtcgctgcg
     2041 cgatccggat agcagagcgc gaacgagatc ggaactcgca tgtccgggta gccgacatgg
     2101 gccagcgcag caccgtcgtg aaaccggacg agcccatgta cgacggaggt gggatgcacg
     2161 gtcacctcga tctgctcgaa ggggagatcg aagagaacgt gagcctcgag cagctcgagc
     2221 cccttgttgg cgagcgtcgc ggagtccaca ctgatcttac ggcccattcg ccaagttgga
     2281 tgggccaggg cctgctcggc cgtcacgtcg gcaagctcat cgcgcttacg cccacggaac
     2341 ggcccacccg acgcggtcag caccagcgag tgcaccgacg cgagatccct gccttccaga
     2401 cactggaaga gcgccgagtg ctcgctgtcc acggggagca gcggccctcc gccccgctcc
     2461 tgcgcggatc gagcgagctc gccggccgcg acgagactct ccttgttggc cagagcccgc
     2521 gggatcccgg cttcgagcga ccacagggtc gcgcggacgc cggcgaagcc cacgatggcg
     2581 ttcagcacga tgtcgggacg gctctcctcg agcacgcgaa ccggatcgcc gccgatctgt
     2641 gtgtgctcga cgccctgtgt ctcggcaagt ccgtcgagcg gctgtgagcc ggacatgagc
     2701 gcgcacagct cgagctcagg atgagcctcg acgatcccga tcgtctgccg gccgatcgac
     2761 cccgttgcgc cgagaagggc aacccgtctc atggcgtcgt cagtgtagag gcctgcactc
     2821 agcgatttgt ggcaaatgca tcgaggcagc cctgccgtcc ggcggcgaag tcctccgcgc
     2881 cggcggctcc tcccacctgc tcggcgaccg ctctcgcgac tgcctcgcgc gtcggctcgg
     2941 ccccgaacag cccggcgaca tactcgagcg ggccctcctg acagagctcg tagccgccct
     3001 ggtacgacgg gctcgaggtg tcgacatcgc ccgagccgga cggcgccggc gagcttccgg
     3061 ccgagtcgct gccggacgta tcggtcgccg cgccggaaga gctctctccc tctgagctcc
     3121 caccacagcc agcggcgacg ccgaggagcg ccacgaggac gagcaggagg agcagtgggc
     3181 gtcttgtcga gtgcatttcg agtccttcgt tgagggtagc tgccgtattc gctgtcgcgc
     3241 cctcaagcct tgccgaacgc cagaagcacg acgtaggcgg cagggcccgc gaacagaatc
     3301 gagtcgaccc ggtcgagcac accgccgtgg ccggcgagca gcgtgcccga gtccttgact
     3361 gccatgtccc gcttcacggc ggattcgaag aggtcgcctg tgaccgccgc caccgagatg
     3421 gccacgccca gcacgatcga ctcccagatc tcgagatacc cctgctcgta catggcgaag
     3481 aaggccacgg caacggccgc gaccgtaccc ccggcgaatc cctcccacga tttccccggg
     3541 gagatcgcgg gcgccatctt gtggcggccg atcaggcgcc cgacgaagta ggcgaacgtg
     3601 tcgtcagcga acactgccag caagaccgtg aagatcgcca gacgtccgtg ctccgggatg
     3661 tcgcgcacga gcaggagtaa gccgatcccg atcccgaccc aggcgacgcc gagcagcgtc
     3721 gttccgaccg cggtattgaa cgagggccgc gcgtcggaga tcccgtagag gaagaacgcg
     3781 agtgggaagg tggcggcgat gcccgcgagc agccacggcg cgcccccgag ctgtgtgccc
     3841 agcagcgcaa gcacgagagc gacgtagccg ccgaggacga gcgggcggag agacttcgtg
     3901 atccggtagt actcgtgcaa ggcgatcaga gatcccgcta gggccagccc ccacagccac
     3961 cagccgccca cgtacacgag gccgagaacg agagggagca aggcgatcgc gacgagaatc
     4021 cgcgaccaga acgccgacat cgatcagcgt cctccgaaac gtctttgccg acgggcgaac
     4081 tcgtcgagcg cgtgtcgcag gtctacctcg tcgaagtcgg gccagagcgt atcggtgaag
     4141 acgagctcgg cataggcggc atgccacagg aggaagttcg agatcctctg ttccccggaa
     4201 gtgcggacca ggaggtcgat gtcgggaacg tcgggagcgt cgagacgggc agcgtacgcg
     4261 gcttcgtcga tctcgtcggg taagagcgcg tcatcgacca gccgccgtgc cgcctcgacg
     4321 agctccgctc tgccgccgta gtcgaaagca atccagagaa ggaggcgatc gttggtcgcg
     4381 gtggcctcct ccagacgctc catccggcgg cggaggttct cgggcgcacg gtcgcgccga
     4441 ccgatgaagc gcgtccgcac accctggcgc tgcaagtcgg gaagctcccg ctcgatcgtc
     4501 tccccgagga tctccatcag cgattcgatc tcatcggccg gacgcgtcca gttctcggtc
     4561 gagaaggcgt agacgcagag cgacttgacc ccgaggtcga tcgcggcctc gacagtgcgc
     4621 cgcagagccc gtgtgccggc gcggtgtccc tcggcgacag gtaggccccg gttctcggcc
     4681 cagcggcgat taccgtccat gatgatcgcc accgagctgg ccgatggtgt cgaagaacgc
     4741 gccggcgccg gcgtggcctc ggtgtcggtg ctcactgctc ggaaggcgcg gagggcaccc
     4801 cctacacctc catgatctcg tcttccttgt gcttcaggag ttcgtcgatc cgaccggtgt
     4861 ggtcgtcggt catcttctgc acctgcgtct cgccccggcg ttcctcgtcg tcgccgacct
     4921 cgccgttcgc gacgagatct ttcagatggt gcatgacgtc cctgcggacg ttccggatgg
     4981 cgaccttgcc gtcttcggcg taccgacgga cgaccttgac cagctccttg cgcctctcct
     5041 cggtcagctg cgggatcggc aggcggatca ccttgccgtc gttcgagggc gtgagtccca
     5101 gatctgactc ctggatcgcc ttctcgatcg ccttgacctg cgtcgggtcg tagggctgca
     5161 ccgtcaggag tcgcggctcg ggaaccgaga tcgtcgccat gttcttcagc ggcgtaggag
     5221 ttccgtagta gtcgaccgtg atgcggtcga gcagcgccgc cgaagcgcgt ccggtccgga
     5281 cggtgttgaa ctcgtttctg gtcgcctcga tcgacttgtc cattcggcgt tctgcgtccg
     5341 cgatgaagtc ctcgaccgct cccatctaga cctcctttcc aggtgtggag acgcctagcg
     5401 tctccgtcga gatgatcgtc ccgacccgct cgccgcctgc gaccttcttg atgttcccgt
     5461 cctggagccc gaagacgaag atcggcagcc cgttctccat gcagagggag agggcggtgg
     5521 tgtccatcac gcgcagcccc cgctcgatcg cctccaagtg cgtcagccgc ggaaggaagc
     5581 gcgccgatgg gtccgagcgc gggtcgccgt cgtgcacccc ttcgacgccg tacttcgcca
     5641 tcagcagtgc ctccgcaccg atctcgagcg cccggagcgc ggcagcagtg tcggtcgtga
     5701 agaacgggtt gcccgtcccc gcggcgaaga tgacgatccg ctccttctcg agatgccgga
     5761 tcgcgcgacg gcggatatac ggctccgcga cttccgagac gtcgatcgcc gactgcactc
     5821 gcgtgtccgc gccgagacgt tcgagtgcgt cctgcaaggc aagtgcgttg agcagggtcg
     5881 cgagcatgcc gccgtaatcg gcggtcgcgc ggtccatccc gctgtcggcc gcctcggcga
     5941 gccctcggta gaagttccca ccgccgacga cgactgcgag ctccgtgccc gccccctgca
     6001 cgcccacgag ctcacgcgcg atggcgttga ccgtttccgg gtcgacgccg tagtcgcgct
     6061 ctcccatcag gctctcgccc gagagcttca gaaggatgcg cgagaagggc tttcccgcga
     6121 cctccgtcgc cgggtgctca gccgtttcgg agcgcgcact cacgtgccgg caaccgagac
     6181 ccgcgagaac ccggaagcgg tcgccccagc ttcgtcgagc gcctggccca ccgtcttcga
     6241 ggcgtcgtga atccacgcct gctcgagcag aacgcctccg ggggaggcgg ccaagaaccg
     6301 cttcgccagc atgccgtcca cgatcttctc acgcgcgccc tccggcttcg actgaacttc
     6361 gtcggacgag aggtagatct ggcgctccgc cgcgaccact tcgtcgggca cgtcctcccc
     6421 ggtgacccat tccggcgccg cgaaggagat gtgcatcgcg agctgccggg ccagctcggc
     6481 cgagcccccc tcgaggcgca cgagcacgcc gatcttgttc gccggcgggt gaacgtatga
     6541 cgcgaggacg ccgccgtccg catcgaaacg ctccgcgcct accacgacta tgttctcgcc
     6601 gaggcgcgcc gacagctccg tccgctcgcc gtcgagtccc tccgttgctg taggcccgcc
     6661 atcgagcacc gctccgacga cgcgcgacac gaactctcga aactcgtcgt tcttcgacac
     6721 gggctctgtc tcgcagccga cgccgacgat cacgccgacg ccgttgtcga cactggtctc
     6781 gacgatgccc tctgtcgtcg cgcgacctgc gcgcttggca gcagaggcga ttcccttctc
     6841 gcgcaggatg cgacgcgcgt cttcgaggtc gccgccggac tcttggagcg ccctcttgca
     6901 atccatcatt ccggcgcccg tcatgtcgcg cagctccttg acgaggctcg cgctgatctc
     6961 ggtcatgcct tctcgctccc ttccgccacc tcgacggcct gtccttcttc ggccggctga
     7021 gcgggctgtg cctcgtccgc tgccgcggcg ggctcgaccg cctcctccac ctcgggcgcg
     7081 gcagcctgct cggactcggg cgccgccccg gcggcggaat ctccggccgc ctctggtgtg
     7141 cgttctgcgg ggctcggcgt tggggtctcg agttcctcta ccgtcacctt cgtcgcgccc
     7201 gcggcgattc cgtccgcgat agcccggatg atcagtccac acgagcgcaa cgcgtcgtcg
     7261 ttgccgggaa tcacgaagtc gacctcgtcc gggtcgcagt tggtgtcgac caacgcgatc
     7321 acgggaaggc cgagccgcct cgcctcgttc acggccagct gctccttccg gaggtcgacg
     7381 atgaacacgg cgtccggctg cttgcgcatg tccgctacgc cgccgaggtt cgcctccagc
     7441 ttctcgagct cgcgctccag cgagatccgc tccttcgccg gcagcaggtc gagctggtcg
     7501 tcctccttca ggcggcgcag gtcgtggaga tacgagatcc ggtcggagat cgtgcgccag
     7561 ttggtcagca ggccgcccag ccagcggttg ttcacgtacg gcatgccgac acgcgacgcc
     7621 tcctcgacga tcgcgtcctg cgcctgcttc ttggtgccga cgaagagcac ggtgccgcca
     7681 cgctcggcga gattccggac gaagtcgtat gcggtctgca ccagcgtttg cgtctgctgg
     7741 aggtcgatga tgtagatgcc gccacgctcg gtgaagatgt agcggcgcat tttcggattc
     7801 cagcgccgcg tctggtggcc gaagtgcact ccagcctcca ggagctcctt catggagacg
     7861 actgccaaga gaccagcccc tttcgttggt tttgtcgaag ctgccggcgg ggcacggacg
     7921 gaaccgggcg cgagggccgc ggcaccaccg tccggctgat ccaccgggtc ggtaggacac
     7981 gaactcgcgc catggtagcg cctacggctc gtgcgcccgc gcgagcgcgc tctcgagatc
     8041 tggtggcagc ggcgaccgta cgtccagctt ctccccggtg aacggatgcg tgagacggag
     8101 acgatgcgca tgcaggaact ggcgggcgag gcccaagtcg cctttgacgc cgtagacggg
     8161 atcgcccgcg accgggagct cgatcgcctc gagatgcacg cggatctggt gcgtccgacc
     8221 ggtctccagg cgcacctcga gcagggcatg ggccggcagc agctcgcgaa gctcgaacca
     8281 ggtgaccgcg gctcgaggtg tgtgcgtgtc gattgagtgt cgtcttcgat cgacccggtc
     8341 gcggccgatc ggggcctcga ttcgcccggt ccgcgaacgg ggagcgccac gtacgagcgc
     8401 gagatagcgg cgatcgatcg cgcgacggcg catcagcccg gtgagccgcc ggtgcgcatc
     8461 ctccgagcgg gcgacgatca tcaaccccga cgtgtctcgg tcgagccggt gcacgatgcc
     8521 gggcctcccc tcctcgcccc ctttcgcgcc caacccgacg agcccgtgga cgagcgtgcc
     8581 cgaccgcgct cgcccggccg ggtgcaccac caggccggcg ggcttgtcga ctacgaggag
     8641 atgctcgtcc gagtacgcga ccgcgacatc gagcgcctcc tcgtccagca ccgtggtaac
     8701 cgggagctga gcctctacct gctcaccgcc ggcgagccgg tgactcttgg cccgctgcgc
     8761 accgtcgacg gtgacctggc ccgagccgat gaggcgctct gcgacgctgc gggcgccgac
     8821 ttccgggcgc cccgcgagga ccaggtcgag gcgcttgccc gcgtcgcggt caggaacgcg
     8881 aaagcggagc tctgccaacg cgcgcgctcg tgcggtcggc caccacaaac gagacgaaca
     8941 gcaggccgac tccggcgacg atgaaggtgt cggcgaggtt gaaggccggc caatggtcga
     9001 agtcgaggaa gtccgtgacg tagccgaagc ggagccggtc gacgagattc gaaaggctgc
     9061 cgccgagcac gaaccccagg gcgaccggaa gcatcggatg gcgctggccc gagcgcgcga
     9121 agaacacgac catcgatccg acggcgagcg tcgtgagaac gatcacgatg gcggtggcgc
     9181 tcgagaacaa tccgaaggcg attcccgagt tgcggacgtg atggatggaa aacggaccga
     9241 ggatgtcgac ggacttcccg aggccgagag cactggccac gacgcgcttc gtcagctggt
     9301 ccgctcccac caccgccccg gcgacgagga ggagcgacag ccattgaacc ggcccggcac
     9361 ggagcgagcg ctccgccgac gagatcggct cgagcgcgtt cgtcgtcgaa cccacgcgaa
     9421 cgtcgagccg gcgtcgctcc acgctccgct gcccgagcgg ctcgctcaac cgcgctcctg
     9481 cttgcgcttg tcctcgatgc agagggtggc gtacggcacg gcctcgagcc gttcctcggg
     9541 gatcggctcg ccgcagaccg agcaaaggcc gtatgtcccg gcggcgatcc gcgcgagcgc
     9601 atcgtcgatc tcttggagaa cgtgctccgc gttctcctcg agggtttcgt cgagctcgcg
     9661 gtcgaagata tcgctggcat ggtcggccat gtgttgatcg ccgctcgggg aactcagctc
     9721 gccggcgttg tcatccgcgt gttcgacgta ctctgcggcg gccgtcagtc ggtcgcgcaa
     9781 atcgagcagc tcggccttgc gggccttgag atccagggtc atccgttctc ctttccgtcg
     9841 gctccctcca gccgccgctc agcttcttcc aggctcatcc ccgcgacctc gaccaccttg
     9901 tcacgggcac ccgcgccgga gacgacgcga acgtctggcc gcgccacgcg cagcctagcg
     9961 gccaagaggt ctacgacggc gtcgttggcc cgtccgcgtt cgggagccgc cgcgacccgc
    10021 accttccatg cctgcccgta gcggccgacc accttcgacc gtgcagcccc tgggctgacg
    10081 cgcaggcgca aacgtgccgt tgaggcgtcc acaccccaag gatatccggg ctatccggcc
    10141 agcctgcgga cgtccgggtc ggtctcgtcg cccgggccct cgcgccggtc gtcgtcactc
    10201 ccgccggctg ccgccgtcgg agtgccgctc tcggatgccg gcgggagcgg cgaggctggg
    10261 cgttgcgctt ccgccggtgc atccgtgccc aatggctcgg ccggtgcggc gtcgcgggct
    10321 tcgtcgacga cttcgagcgc gctgcgaagg atcgcctgta cacgctgcgc ctccgccagc
    10381 agacgctgtc gttccgcgag tgcgtcccgc ttgatcgaac gggcctccga gtgcgcctcg
    10441 ttgacgatca ggtctgcctc tttgcgcgcc cgctctttca cctcttctgc tgcccgctcc
    10501 gccgatacga gcgtttgccg gagcagaccc tcgagctcga gatggcgttt gacctcggcg
    10561 tcgaggactt cgacccggtc ggcgagctcg gcccgctcac gccagaccag ctcgaaactg
    10621 tcggcgacct cggtcatcag gcggtccacg gccgcccgct tgtagccgaa cagcgttcgt
    10681 ttgagctctt gatgacggat ttcgactggt gtgagcggca tggtgccctc cattgttgct
    10741 tagagaacgc gttcgatgag cgcgaccaag aacctttgca cgattacgag cacgaagatg
    10801 ccgacgatcg gagagaggtc gagcggcccg agcggcggca ggactcgccg gaagagccga
    10861 agatatggat cgcagacgtc gaagaggaag cgctggattc ggctgagtgt cggcgagtac
    10921 ggaagcctga tccaggacgt gatgatgtac gcaaagatca gaaggatgta gacgtagatg
    10981 aagacctcga cgaacgtcgc gatcgacgac gcggcgtcgg cgagcaagcc aagcacggcg
    11041 aggcagccgc ccatggtcat cgtttcggca ccctaacgcg ccttcctacc gcggttgtca
    11101 ggatctgccg gccgcagtga ctacgcctga ttgaagaaac cgccacgatc gagcatgcgc
    11161 gcacgctcct cggcggagac ttcgacgttg cggggcgtga ggagaaagac cttctcggcg
    11221 acgcgttgca tgctgccgtc caacgcgtag gtcagaccgc ttgcgaagtc gatcaggcgc
    11281 ttcgagagct cgttgtcggc gctctggagg ttgaggatga ccggaacctg atctttgaag
    11341 cggtcggcga tctgctgcgc gtcgttgaag ctgcggggca ggactaggag cacccgaacc
    11401 tgtcccccgc cctggtcgac gccgcgaagc cgtgtgcgac gcgtgcgcgt ggcgacggcc
    11461 gacggctcgg agtccatgtc ggtgtcagcc cactcggtcg gctcctggcg tcgcggcagg
    11521 cgcctcacgt tcgcgcgctc ccgtgaccgg taatcgcgcc cgagctcttc ctcggtcgca
    11581 tacccgtcct cgtcccactc ctcgtcctcg gcaatgccga agtacacgag cgtccgactc
    11641 cacacttccc cgaatcccat tacagcgccg aagtttagat gacctgccgg gaattccggc
    11701 tcggttagcc gcgtagcagc accgatccga cgcgaatgag tgtcgccccc tcctcgacgg
    11761 cgacgcgcca atcttgcgac gtccctatcg agagctccgg cagcgcgtac tcagctgcaa
    11821 gccggcgcag tctctcgaag aacggacgtg atgattccgg atcgtcggac agaggcggca
    11881 tcgtcatcag accgcgaatc aacgggtatt cgtccagata gcggggaacg tcggcgggcg
    11941 gcacgccgga cttcgacggc tcgcccgaga ggttcacttc gagcagagcc ggaatctcca
    12001 gtcggcgcgc cgccgattcc gagctcagcg aatggacgag ctcgcagagg cgattcacga
    12061 ccttcacctt gttcgactgc aggtgcccga tgaagtgcca gcggaaggcg gagccgtaga
    12121 cggcgtgctt ccgctccagg tcctgcgctc ggttctcccc gaccacctcg acgcctgcct
    12181 ccgccagcat cgccatgtcc tcgagcgcga cgtacttggt ggcggcgaca acggtcacgt
    12241 cgggcccggt ggcctcgcgg atctcgtcgt aggcgtcacg aactccagcc ggcgtcatgc
    12301 gagttccttg cgggcaaggg cgagcagagc gtctttttcg ttctgcgagg gatcgaccag
    12361 cacctggtcg agcaggcggc gcagaagctc gcccaccgcg ggaccctcgg ccaaccccag
    12421 ctcgagtagg tcgtccccgt cgaccgcgag atcggcgagt cggtgggggc tggcgacctc
    12481 atgctccagc agtgcgcgaa agcgctgaag gatcccgtac tcctccgtgg ggacgccctt
    12541 ggcggcgagg tcggcctcct tgagcgcgac cagctcgaag gcgatctcat ccccgtgcgc
    12601 cgcgagaaac cgacgcgctc tggccggatc gatctcggcc cgaggcgaga acgcatgcgc
    12661 cgccacgatg gccgtcaccc tacgttgcag ccgtacgggg tagcgcaggc gctcgagaat
    12721 cgttcccgcc ct
//
LOCUS       DBNGNK010000177         1980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1247055,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000177 DBNGNK010000000
VERSION     DBNGNK010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1247055"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /locus_tag="AC150V_05140"
     CDS             complement(<1..496)
                     /locus_tag="AC150V_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762342.1"
                     /translation="MRRRGASLLAVVAAVLVTAGGAAAVDFGATDDTGKYLGADSAPY
                     FSAMRQVGLTVNVMTLTFDGSLRISPEETAAMDSALPVAQRQGITVALRLYPDRPTTI
                     GTSAVLAKRFAKWAAKVARRYPSIQTFVIGNEPNQPRFWQPQFDRRGRQVSAAASGRM
                     LAFTY"
     gene            complement(539..1498)
                     /locus_tag="AC150V_05145"
     CDS             complement(539..1498)
                     /locus_tag="AC150V_05145"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN family protein"
                     /protein_id="HIR3762343.1"
                     /translation="MHGVLRYGSDDVVAILDSTRAGETHDGIPVIAGVNDALCFDPNV
                     ALVGVVTQGGRFPPHWRELLKACIRQGLDVENGLHEFLADDPELRDLAVRFGVELRDL
                     RRPPDGLDCPTGENLALAARIVLTVGSDCAIGKMTVSLELDGEARRRGLGSVFVPTGQ
                     TGIAIAGWGLSVDAVIADYVAGAAERLVVEGYRRGGELLFVEGQGALVHPAYAGVTLG
                     LLHGSAPHALVLCHRAGSREVDGYPGHPLPPLSDLVRLYENISLPIRPARVACIALNT
                     MGLADAEAREAIARTEAETGLPADDPVRFGPARLLDTVLVALD"
     gene            complement(1562..>1980)
                     /locus_tag="AC150V_05150"
     CDS             complement(1562..>1980)
                     /locus_tag="AC150V_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014393695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR3762344.1"
                     /translation="AALGVEYVEQPLPAGDEGGTELRERSPLPIYVDEDCHVAADVPA
                     CARVAHGVTVKLAKSGGIREALRAVHAARALGLGVMLGCMPESGLGIAAGCAVAPLCD
                     HVDLDGNLLLRNDPCPGVELVEGVQVPPGSPGLGVG"
BASE COUNT          316 a          694 c          692 g          278 t
ORIGIN      
        1 cgtacgtgaa ggcgagcatg cgacccgagg cggccgccga gacttgtctc ccacggcggt
       61 cgaactgcgg ctgccagaac cgcggctggt tgggctcgtt gccgatcacg aatgtctgga
      121 tgctcgggta gcggcgtgcg accttagccg cccatttcgc gaagcgcttc gcgaggacgg
      181 cgctggtgcc gatcgttgtc ggccgatccg ggtagaggcg gagagcgacc gtgatcccct
      241 gcctctgggc gacggggagc gcgctgtcca tcgcggccgt ctcctccggc gagattcgca
      301 gcgagccgtc gaaggtgagc gtcatcacgt tgacggtgag cccgacctgc cgcatagccg
      361 agaaataggg cgcgctgtcg gcgccgaggt acttgccggt gtcgtcggtg gcaccgaaat
      421 cgaccgcggc ggcaccgcca gcggtcacga gtacggccgc aacaacggcc agcaaactgg
      481 ctcccctacg cctcacgttc ccatcgtttc gcggtctcag aacgcatccc ttccaggctc
      541 agtcgagggc gaccaacacc gtgtcgagga ggcgcgcagg gccgaaccgt accggatcgt
      601 ccgccggcag tcccgtttcc gcctcggttc gcgcgatcgc ctcgcgggcc tcggcgtcgg
      661 ccaagcccat cgtgttgaga gcgatacacg cgactcgcgc cggtcgaatc ggcaagctga
      721 tgttctcgta gagccgaacc aggtcggaaa gcggcgggag cggatgaccc gggtagccgt
      781 ccacctctcg cgagccggct cgatgacaga gcacgagcgc gtgtggcgcc gatccatgga
      841 gcagcccgag tgtcacgcct gcgtaggcgg ggtgaacgag cgctccctgc ccctcgacga
      901 agagcagctc gccgccacgc cgatacccct cgaccacgag gcgctcggca gccccggcga
      961 cgtagtcggc gatgacggcg tcgacggaga gcccccagcc cgcgatggcg atacccgttt
     1021 gacccgtcgg cacgaacacc gagccgagac cccggcggcg ggcttcgccg tcgagctcga
     1081 gcgagacggt catctttccg atcgcgcagt cggagccgac cgtgaggacg atccgcgcgg
     1141 ccagggcgag gttctcgccc gtcgggcagt cgagaccgtc cggcggtcgt cgcaggtcgc
     1201 gcagctcgac gccgaagcga acggccagat cacgcagctc gggatcgtcc gcgaggaact
     1261 cgtgcaggcc gttctcgacg tcgagaccct gccggatgca ggccttcaac aactcgcgcc
     1321 agtgcggcgg aaagcggccg ccctgggtga cgaccccgac gagggcgacg ttcgggtcga
     1381 agcagagagc atcgttcacg cccgcgatca cggggatgcc gtcgtgggtc tccccggcgc
     1441 gcgtggagtc caggatcgcg acgacgtcgt cgctcccgta gcggaggacg ccgtgcatcg
     1501 tcttcccgta gtgggcgtcg gccgagaagc cctcggccag gatgaggagg cgacgggaga
     1561 tctacccgac tcccagcccc gggctgccgg gcggcacctg gacgccctcg acgagctcga
     1621 cgccggggca cgggtcgttc cggagcagca ggttgccgtc cagatcgacg tggtcgcaca
     1681 gcggcgcgac ggcgcagcct gctgcgatcc cgagccccga ctcgggcatg cagccgagca
     1741 tcaccccgag cccgagagcg cgggccgcgt ggacggcgcg gagcgcctcg cggatcccac
     1801 cggacttcgc gagcttgacc gtaacgccgt gcgcgacgcg cgcgcacgcc gggacgtccg
     1861 ctgcgacatg acagtcctcg tcgacgtaga tcggaagcgg cgaccgctcc ctcagctctg
     1921 ttcctccctc gtctccggcc ggcagcggct gctcgacgta ctccacgccg agcgcggcga
//
LOCUS       DBNGNK010000178         5978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_819455,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000178 DBNGNK010000000
VERSION     DBNGNK010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_819455"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="AC150V_05155"
     CDS             <1..325
                     /locus_tag="AC150V_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR3762345.1"
                     /translation="YASRATDALERLAGVGARVELEPDSALDDVDKHGRLLRYVRVGS
                     DNVNVLLVEQGAAAPYFFRGDRGRYAEQLLDAAESARAQNRGLWGACPGAALEPELGS
                     RTGRR"
     gene            complement(247..837)
                     /locus_tag="AC150V_05160"
     CDS             complement(247..837)
                     /locus_tag="AC150V_05160"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR3762346.1"
                     /translation="MAFIFVESPHRTRAYGRAIDLVVIHSMEVAERPDAAEVCAQWFK
                     HPSAEVSAHYCVDADSVVQCVRERDIAWHARGGNSRSIGIELAGFARQANADWLDDYS
                     CAVLERAATLTADICHRYAIPVRRVRAAGLRKSVRGITTHADVSAAFGKSDHWDPGPG
                     FPMIRFLELVRQRLPVLEPSSGSRAAPGQAPQRPRF"
     gene            950..2485
                     /locus_tag="AC150V_05165"
     CDS             950..2485
                     /locus_tag="AC150V_05165"
                     /inference="COORDINATES: protein motif:HMM:NF051130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HIR3762347.1"
                     /translation="MSRERTPRFRELQQRLRSIWPTVTPRSIGDVERTVVVCYSLTLE
                     LPAPLLPALSAFEERFLCVVLTLLRSPRTRVIYLTSIPVLPRLVDYWFSLVPELDTPS
                     ARERLLLLSPVDSRPLPLTRKVLDHPGLVERVREAIPDPSTAFLVPFLSTELERELAV
                     RLGIPLYGPDPALTHFGTKSGSRRLFREAGIALPAGVEGVRTRAEVVAAIDEIRAERP
                     ELPGVVVKLDEGVSGYGNATVHLDADTTAAAAVDRPELVDPDRDADDFYEALEREGGI
                     VEEMIVAEDVRSPSVQLRLGPDKTIAVISTHDQVLSGPQGHTFVGCRFPAETAYVFEI
                     TEASKRVGELLCREGVIGRLAIDFMAVRSEYGRWTPYALEINLRNGGTTHPTVTLQAL
                     TDGHYDADRGVFFDPDGRQKCYFATDHLERPGYELLTPDDFLDLLDESGLAWSAEDHT
                     GVVFHMLSAISVAGLVGVTAIADSQHEAYALYRRARDAVDQEAARLTALADDVETAAR
                     KDG"
     gene            2482..2967
                     /locus_tag="AC150V_05170"
     CDS             2482..2967
                     /locus_tag="AC150V_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR3762348.1"
                     /translation="MTDPLWAPWRLDYVQQAGEESGCFFCREAAGELGEDSLVLRRGE
                     TVIAMLNKFPYSSGHVMVAPIRHVAGFDEIEPDEWLEIYDLTAAAMSGLTEIYRPDAF
                     NVGWNLGEIAGGSVAGHLHQHVVPRWAGDTNFMPVLADVKVVPEHLLVTRDRLVAAWP
                     T"
     gene            3062..3718
                     /locus_tag="AC150V_05175"
     CDS             3062..3718
                     /locus_tag="AC150V_05175"
                     /inference="COORDINATES: protein motif:HMM:NF021996.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR3762349.1"
                     /translation="MSTPTTQANSLPRALPAFLRLLAVPAAISLVLAGIWATGGVVTD
                     DFRASMALTALWFGAVVIGAVAAWRSSRGLRPAAVAAVATFILVGAWLGLASIRDVTV
                     NEQVAAGPALVSGSFRGLAHPTEGVVRIVEQPAGSRVVTLTSFRTDPGPDLYVYAIPG
                     RSDGSDVDGGIRLGRLKGNVGNQQYGVPADFPSGPATVVVWCRAFSVSFGAAQLAAPG
                     "
     gene            complement(3715..4581)
                     /locus_tag="AC150V_05180"
     CDS             complement(3715..4581)
                     /locus_tag="AC150V_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762350.1"
                     /translation="MRVAVLALSLLVALAVAGAVPAQQESTSPLDGLGTWVDIYDGGV
                     FANPERTADRIAAQGVRTVWAETANDRAKSDVVRPQKLGQLVDALHARGIEVVAWYLP
                     GFVKPGLDLRRTRAMLRFRTPTGGAFAAVALDIESPRLANAKLRSKRMLALSKRLRAE
                     AGDIPVYAITLPPRMLERRPTAWPDFPWAELATLVDGFVPMTYTGSAFEGYETTYGYV
                     ARSLALLRALTGRPDLPIHAAGGVANRMQEDELLAFTDAVADDGRVRGWSLYDYATTG
                     PASWRAITALGS"
     gene            complement(4625..5362)
                     /locus_tag="AC150V_05185"
     CDS             complement(4625..5362)
                     /locus_tag="AC150V_05185"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR3762351.1"
                     /translation="MTRDLGFPTVPAEAVPWLTVEEMREVDRIMIEELGITLVRMMEN
                     AGRGLAVLARRLLGGDASGRRVGVLAGRGGNGGGGLVAARHLAVAGARVSVVLSAEPG
                     RLAPVPGEQLAILERMGVPVAVEPSGLGEPELVLDALLGYRAKGPPRGETAALIRYVL
                     GKRLLALDVPTGLELATGVLHEPYAAAEATLTLAAPKHALGDRAAKTAVGDLYLADIS
                     VPAVVYDRLGRSHASPFATGPIVRVSD"
     gene            5398..5470
                     /locus_tag="AC150V_05190"
     tRNA            5398..5470
                     /locus_tag="AC150V_05190"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5431..5433,aa:Gly,seq:ccc)
BASE COUNT          866 a         2150 c         2031 g          931 t
ORIGIN      
        1 ctacgcgagt cgtgccaccg acgcgctcga gcggctggcc ggcgtcggtg cccgagtcga
       61 gctcgagccg gactccgcgc tcgacgacgt cgacaagcac gggcggctcc tgcgctacgt
      121 ccgggtcggc agcgacaacg tcaacgtgtt gctcgtagag caaggcgccg cggccccgta
      181 cttcttccgc ggtgaccgcg ggcgctatgc ggagcagctc ctcgacgccg cggagagcgc
      241 acgcgctcag aaccgcggcc tctggggcgc ctgccccggc gccgccctcg agcccgagct
      301 cggctccagg accggcagac gctgacgaac gagctcgaga aacctgatca tcgggaagcc
      361 cggccccgga tcccagtgat cgctcttccc gaaagcggcg ctgacgtccg cgtgcgtcgt
      421 gatgccgcgg acgctcttgc gcagcccggc ggcgcgcaca cggcgcacgg gaattgcgta
      481 gcggtggcag atgtccgcgg tgagcgtggc ggcccgctcg aggacggcgc agctgtagtc
      541 gtcgagccag tcggcgttcg cctgccgcgc gaaaccggcc agctcgatgc cgatgcttct
      601 gctgttgccg ccccgcgcgt gccacgcgat gtctcgctct cgcacgcact ggacgacgct
      661 gtccgcatcc acgcagtagt gggcggacac ctctgccgac ggatgcttga accactgcgc
      721 gcacacctcg gcggcgtcgg gccgctcggc gacctccatc gagtggatca cgaccaggtc
      781 gatggcgcgt ccgtacgcgc gcgttcggtg cgggctctcg acgaagatga acgccacgac
      841 tccccctttt cccttgggtc ttgccgcagc cgcaccgaca ttacgcgatc ggcccatcgc
      901 tgccaaggac cggcgtgacg gaggcagctc gagacgctac ggtcggccgg tgagccgcga
      961 gcgcacaccg cgttttcgtg agctgcagca acggctgcgc agcatctggc cgacggtcac
     1021 gccgcgcagc atcggggacg tggagcggac ggtcgtcgtc tgctactcgc tcacgctcga
     1081 gcttcctgcg ccgctcctcc ccgccctcag tgcgttcgag gagcgctttc tctgcgtcgt
     1141 cctcactctg ctccggtcgc cacgcacgcg ggtcatctac ctcacgtcga ttcccgtcct
     1201 gccccggctg gtcgactact ggttcagcct cgtgcccgaa ctggacaccc cctcggcccg
     1261 cgagcgactg ctgctcttgt ccccggtcga ctccagaccg ctgccgttga cccgcaaggt
     1321 cctcgatcac cccgggctcg tcgagcgggt acgcgaggcg atccccgatc cgtcgacggc
     1381 gttcctcgtg cctttcctct ccaccgagct cgagcgggag ctggccgtgc ggcttgggat
     1441 tccgctctac ggcccggatc cggcgctgac ccacttcggc acgaagagcg gcagtcgccg
     1501 cctctttcgg gaggcgggca tcgcgctgcc cgccggggtc gagggcgtca ggacacgcgc
     1561 cgaagtcgtc gccgcgatcg acgagatccg agccgagcgc cccgagctcc ccggggtcgt
     1621 cgtcaagctc gacgagggcg tctccgggta cggaaacgcg acggttcacc tcgacgcgga
     1681 cacaaccgcc gcggccgcgg tcgaccggcc tgagctcgtc gacccggatc gggacgccga
     1741 cgacttctac gaggcgctcg agcgcgaggg cggaatcgtg gaggagatga tcgtcgccga
     1801 ggacgttcgc agcccaagcg tgcagcttcg gctcgggccg gacaagacga tcgcggtcat
     1861 ctcgacccac gaccaggtgc tgtcgggccc gcagggccat acgttcgtcg gctgccgctt
     1921 cccggcagag accgcctacg tcttcgagat cacggaggcg tcgaaacgcg tcggagagct
     1981 cctctgccgc gagggggtga tcggccggct ggcgatcgac ttcatggcgg ttcgttcgga
     2041 gtacgggcgc tggacgccgt acgcgctcga gatcaacctc cgcaacggcg gcaccacgca
     2101 cccgacggtc accctgcagg cgctgacgga cggtcactac gacgccgacc ggggcgtgtt
     2161 cttcgacccc gacggacggc agaagtgcta cttcgccacc gaccacctgg agcggccggg
     2221 ctatgaactg ctcacgcccg acgacttcct cgacctgctc gacgagagcg ggctcgcgtg
     2281 gtcggccgag gatcacacgg gcgtcgtctt tcacatgctg agcgcgatct ccgtcgccgg
     2341 tctcgtcggc gtcacggcga tcgccgactc gcagcacgag gcctacgccc tgtaccgtcg
     2401 ggcgcgcgac gcggtcgacc aggaggccgc ccgtctgacg gcgctggccg acgacgtcga
     2461 gacggctgct agaaaggacg gatgaccgac ccgctctggg cgccctggcg gctcgactac
     2521 gtccagcagg cgggcgagga gagcggttgc ttcttctgcc gcgaggcggc cggggaactg
     2581 ggcgaggact cactcgtcct ccggcgcggc gagaccgtga tcgcgatgct gaacaagttc
     2641 ccgtactcgt ccgggcacgt catggtcgcg ccgattcgcc acgtggccgg cttcgacgag
     2701 atcgagccgg acgagtggct cgagatctac gatttgactg cggcggcgat gtccgggctc
     2761 acggagatct accggcccga cgccttcaac gtcggctgga acctcggcga gatcgccggg
     2821 ggcagcgtcg cgggccatct ccaccagcac gtcgtcccgc gctgggccgg cgacacgaac
     2881 ttcatgccgg ttctcgccga cgtcaaggtc gtgcccgagc acctgctcgt gacgcgcgac
     2941 cggctggtcg ccgcctggcc gacgtaacca gccgtaccgg ctcggctccc gccggcacgt
     3001 gttcagctac gtgtaagcgc ctataacgcc cgggcaaccg gccgggacgt acaacgtggg
     3061 tatgtccacc ccgacgacgc aggccaactc gctgccgcgc gccctccccg cgttcctgcg
     3121 cctcctcgcc gtaccggccg cgatttctct cgtgctggcc gggatctggg cgacgggcgg
     3181 cgtcgtcacg gacgactttc gcgcatcgat ggcgctcacc gcgctctggt tcggcgcggt
     3241 ggtgatcggc gcggtcgcgg cctggcgctc ttcgcggggt ttgcgtccgg ccgccgtcgc
     3301 cgcggtcgcc acgttcatcc tcgtcggcgc ctggctcggc ctggcctcga tccgcgatgt
     3361 caccgtcaac gagcaggtcg ctgcggggcc cgcgctcgtc tcaggatcgt tccgcggctt
     3421 ggcccacccg accgagggtg tcgtccgcat cgtcgagcag ccagccggct cgcgggtcgt
     3481 gacattgacg agtttccgca ccgatcccgg ccccgacctg tacgtctatg cgatccccgg
     3541 ccggtcggac ggctcggacg tcgacggcgg cattcgtctc ggacgactga aggggaacgt
     3601 cggaaatcaa cagtacggcg tcccggcgga ctttccgtcg ggcccggcca cagtcgtcgt
     3661 ctggtgccgc gcgttctccg tctcgttcgg cgccgcacag ctggcggcgc ccggctagga
     3721 gccgagcgcg gtgatcgcgc gccagctcgc cgggccggtc gtcgcgtagt cgtagagact
     3781 ccagccccgg acgcgcccgt cgtcggcgac cgcgtcggtg aaggccagca gctcgtcctc
     3841 ttgcatccgg ttggcgacgc cgcccgcggc gtgaatcggc aggtccggcc ggccggtgag
     3901 cgctcgcagg agcgccagcg agcgcgccac gtagccgtac gtcgtctcgt acccctcgaa
     3961 ggcggagccg gtgtacgtca tcggcacgaa accgtcgacg agcgtcgcga gctcggccca
     4021 ggggaagtcg ggccacgctg tcggtcggcg ctccagcatc cgcggcggca gggtgatcgc
     4081 gtagaccggg atgtccccgg cctccgcccg gagccgcttc gacagcgcga gcatcctctt
     4141 cgagcgcaac ttggcgttcg ccaggcgcgg cgactcgatg tcgagcgcaa ccgcggcgaa
     4201 ggctcctccc gtcggcgtcc ggaagcggag catcgcccgt gtccggcgga ggtccagtcc
     4261 cggcttgacg aagcccggca ggtaccacgc gaccacctcg atgccgcggg cgtggagcgc
     4321 atccaccagc tgtccgagct tctggggcct gacgacgtcg ctcttcgccc ggtcgttcgc
     4381 ggtctctgcc cagaccgtac gcacgccctg cgccgcgatg cggtcagccg tccgctctgg
     4441 gtttgcgaag acgccgccgt cgtagatgtc gacccaggtg ccgagcccgt cgagcgggct
     4501 cgtggactcc tgctgggcag gcacggctcc ggccaccgcc aacgcgacca gcagggagag
     4561 cgccagtacg gccacgcgca tggcgcgagg atacgcagtc cgtatgaagg atctttgagg
     4621 catctcagtc cgaaacgcgg acgatcggcc ctgtcgcgaa cggactcgcg tgcgaccggc
     4681 cgaggcggtc gtagacaacg gcgggcaccg agatgtcggc gagatagagg tcgcccactg
     4741 ccgtcttcgc ggcgcgatcc ccgagcgcgt gtttcggggc tgccaacgtc agggtcgcct
     4801 cggccgcggc gtacggctcg tgcagaacgc cggttgcgag ctcgagtccg gtcggaacgt
     4861 cgagggcgag caagcgcttc cccagcacgt accggatcag cgcggcagtc tcgccccgag
     4921 gcggcccctt cgccctgtag ccgagcaggg cgtcgaggac gagctcgggc tcgccgaggc
     4981 cgctgggctc gacagcgact gggacaccca tccgctcgag aatcgcgagc tgctcgcccg
     5041 gaaccggagc cagccggccc ggctcggcgc tcaggacgac gctcacccgc gcgccggcga
     5101 cggcgaggtg gcgtgcggcg acgaggccgc cgccgccgtt ccctcccctt cccgcgagca
     5161 cgccgacccg ccggccggag gcgtcgccgc cgagcaggcg tcgtgcaagg acggcgaggc
     5221 cgcgcccggc gttctccatc atccgtacga gcgtgatccc gagctcctcg atcatgatgc
     5281 ggtcgacctc gcgcatttcc tcgacggtca gccacggaac cgcttccgcc gggactgtcg
     5341 gaaagccgag atcgcgcgtc atcgcgcgtt cttcgacgcg gtagaatcga ctcccgcgcg
     5401 gacgtagctc agttggtaga gcattggctt cccaagccaa gggtcgtggg ttcgagtccc
     5461 atcgtccgct tcgctattcc aagccgtttc cggcgtttct tgctttcggc tcgtgcccaa
     5521 ctagtgccca aaaccctcgg ggttcagtcg cacaccaggt cgtagatagc ccatacggca
     5581 aggccaacgt cgagcgcggc caacaacagg aacgtccgcc gccataccct ggctgcccgc
     5641 cactcgatgg gccgacgcgg ccggaagtat ccccactggg ggtggcgctc gagctttccc
     5701 ggcgcggctt agaggtgtgc catgcgtgtc tcgcggcgtg tcaggcgctg tcaggggctc
     5761 gtcgccgcac cgggacgcgc cgtcgctgcg cggtggcttc tagttcgcta ccgcagacgc
     5821 accgcccagc ccgaaaaggc tgattgacac accaggtttt cgctactcga ccaaggatcg
     5881 acgcaaagct agtggaactc gtgaaccacg gcgtggccct tgccgcggcg aatcatcgcg
     5941 cggttgaccg cgaacgcgaa gggccacgcg atcgcgaa
//
LOCUS       DBNGNK010000179        10413 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1391616,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000179 DBNGNK010000000
VERSION     DBNGNK010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10413)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10413
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1391616"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="AC150V_05195"
     CDS             <1..878
                     /locus_tag="AC150V_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3762352.1"
                     /translation="ISFRAVGIALGLAAAVSWGVADSIAALTGRRVGSLAVVLGFHLS
                     ASVFLLVLVAATGALGDVGWSDVAFFLLLGAVGWIAYLAFYQALALGPVSIVSPISSG
                     YAAVTVILAVLILAERPSGLQWVAVVVVFTGVVFASTDLRELRSLREIHAQGILLAVV
                     AMLLLGALVLGVSSSADDLGWLAPIFLARVFAAAFLGATALARRATFRVPRAAGPLAA
                     IALLGVIETMGFVFFNIGVGHADTAVVATASAPYALVPLLMGVLLLHERPTPTHWVGV
                     VLMISGVVLLGLAAT"
     gene            complement(875..1936)
                     /locus_tag="AC150V_05200"
     CDS             complement(875..1936)
                     /locus_tag="AC150V_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3762353.1"
                     /translation="MKFSYVTLLDYPLADSLEMIKTADELGYYACYAADETWHKDLWL
                     LFAAAADKTTTIRFGPNVSGVYLREPTLICQSLATLDELTGGRVDGVISCGNFGLLSQ
                     YGVDWTKIKPFSRVKEAHHVMRTFLDEGAITYDGEFYKYSGLFTFARPVQERMPLYIG
                     AMRGPKSMQFAGEATDGVHVALSYTKEAYEYAVENVKIGAERAGRDWTSLDIGAWCVI
                     TCGPDSEKAKVAARSIVAFYISSMPEEQLKLHGLDPEEMGEIVAALGAGDLDKAYELT
                     TSEIAETLSIAGTPEECTARIKDFEEAGVNHMICCITDPAILKAFTGKDVDVPDVQGQ
                     LRLIAEQVMPAFAGAGAAA"
     gene            complement(1933..2208)
                     /locus_tag="AC150V_05205"
     CDS             complement(1933..2208)
                     /locus_tag="AC150V_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762354.1"
                     /translation="MIGFRLAAQRPPDAQRITEAVVMRFDHVYEVDPELMTEHTLQQN
                     IPAWDTLRIVDSRWEHLAWMHDHFADSVVSGEELLAEIEAEKKEEKG"
     gene            complement(2205..3716)
                     /locus_tag="AC150V_05210"
     CDS             complement(2205..3716)
                     /locus_tag="AC150V_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3762355.1"
                     /translation="MTTVALSDDVVGELNQICGESHVFTNLSARYNRARVPAPFPLHA
                     WEEHIPDVVVLPTSAEQISEVVKLANRYRLPVVARAGGTGLTDGAVPLRHGIVVDVKL
                     MNKILDIDLVDRTVTVQPGINMLKLNEELRQHGVIYPDNPASYPCSLVGGRIGTGGWS
                     LIGGRYGHTRDLVISFQMVLPTGELIEVGDGGGRKVRKSSTGYQLKHLFMGHQGTLGI
                     VTEATLELVPRPEAEFSAFFWYPDYETAWESTGALAKSGIATLAGVVLFDERKLDYLR
                     RDDEAYIPQPPEVKSVIATAMYGNADEVRAGAKRLLKIGLETGGTYLGDEISHGDWAA
                     RHDRYATPLHGRLRSGQVVPMSWHCEDAAIPYSALPKVREEWHAIADRLIERFEMFDD
                     WGMFAYTNGAYKPWADYLTEIDIGIWEMQWDEESWAAWVEAKRDIAEVAIRHGGSISA
                     CHGACREGEVDLVPLELGGGFDVMKKIKRTLDPNNIMNPGKYLLDEAYEEDEP"
     gene            complement(3718..4941)
                     /locus_tag="AC150V_05215"
     CDS             complement(3718..4941)
                     /locus_tag="AC150V_05215"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3762356.1"
                     /translation="MADGKAPGAGGSTLPPEFLDEWRNAAFNCFSSGHKFCREVCPVM
                     QVTRDEGHTPTAFHANVVAMERGHMSIEDVAEDYVNCTMCGACDIRCPNTLFTGDFYR
                     FRTRTIDVVRAMRTLAVDNGIEQPNWKRWNELTDRWGNEPVLGWNIEVGQNNVRDWAR
                     GLDLPTGGETILFCDCEAAFYRTSVPRSVAQVLRKAGVEFGLMKEQWCCGAPAAEMGY
                     ADLFTSMAEHNVADWRAVGAKRILCLDPHDYIAFTEMYPELFGDDYDFEIVLVVELVA
                     ELIREGKLELTVPVECVATYHDACRLNKRKGVHEAPREILRAIPGLTFKDVDHVTQWS
                     YCSGAGAGFFLERPDIAEEISRRRVEKAAALGVDTLVSACPWSERPLDAQGKEQSIEV
                     WDLMELVAESAGFEV"
     gene            5039..5812
                     /locus_tag="AC150V_05220"
     CDS             5039..5812
                     /locus_tag="AC150V_05220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR3762357.1"
                     /translation="MSASTKPLIGLSVSFHDFGDYQGVGFQRPIALAGGVPLTLPRTI
                     ESLDDALEACDGIVLGGGRDIEPDRYGQAPHETLAATEPHRDAFELELVERALDRGLP
                     ILGMCRGLQLLNVFFGGTLVQDVSLVPAWAEHPSDRGWVRWNEVERASLRGDPDVPEH
                     PRHPITVEPGSLLHDALGVTEIEVDSFHHQALDDVAPNLSVVARAPDGVVEAVELDSG
                     GYVLAVQWELQEEWRVDPRFLAVYERFVAAAAARTEIMG"
     gene            complement(5802..6569)
                     /locus_tag="AC150V_05225"
     CDS             complement(5802..6569)
                     /locus_tag="AC150V_05225"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3762358.1"
                     /translation="MPRTGRPAARQVGSVERAIALLEALAAQESALGTNELARRTGIG
                     PSTVSRLLATLVSGGLVEHVPETGRYRLGLRLLQLGNAVAARLDLREVARPHLEALAA
                     ETGETATLSAPAEPDAVTVDFVQSAALVQGVAELGRPSVSHATATGKVLLAFGDRRLP
                     EGPLEGFTERTVTDPEQLELELGRVRRRGWAEAVEEREPDLTAVAAPVFETGGALVAI
                     LGVQGPSARFTAAARRAARVPLRRHAAEISARLGFSP"
     gene            6620..7933
                     /locus_tag="AC150V_05230"
     CDS             6620..7933
                     /locus_tag="AC150V_05230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3762359.1"
                     /translation="MSGTSFRYVVVGAGIHGLSTAYHLALELRSRGLGSGEDIVVLEK
                     SQPGAGATGIACGVVRNNYFQPAMAELMQHSVEVWESDPVAYHYMPVGYMALGAAVQE
                     GDLVATYERQERIGYSSRLITGEDEVSEYMKGLFPDWRARGVTACLHELQGGFAFNYE
                     SVHGLAAKCGAEGIEIRIGNEVTGFGEAGDGSITAVRTNRGPVEVGEQVVVAPGPWAK
                     SFWQMLGLPDEIDILTPAGERLEGQPMWTYWNLQEGEIAVDPLMFETVDGGAPPVIHL
                     DSDAPLNADDGTLVTDELWGVYFKRDRHGVQGGASPLVVDGDIELDPYPQTTDVDAGF
                     PDMWCAALSHAMARFEGCRPQYMQARSGGVGAFTADNFPVFDYLRPNLYAILDSNHGY
                     KMIGVGKEVARVVTGEHSSLLHPFRFERFATGDLHPVSSSPYPWS"
     gene            complement(8026..8937)
                     /locus_tag="AC150V_05235"
     CDS             complement(8026..8937)
                     /locus_tag="AC150V_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3762360.1"
                     /translation="MARFKKPAAAERVPAASAPGAGRWLRRLVWPALIVAAAVGAFLF
                     ARHQATSGHVPPIEGLPSALDSSALAVGQGADASLVLGTQVGLYVSADGGKEWELGRL
                     DGENVLVLTHSPDGSLWAAGPDIVAHSSDGGASWVEHAASGLAAGGLTGLAAAADGRL
                     YAATKEGLFVSADAGESFEPLAASGADGVTALAAAGDSLVAVAPGRGVLRSEDGGRTW
                     EIVNSERVRSIAASGADPDHVLAAGDGVWISTDGGTTWAESLDPGAPAGAAAWSPRSP
                     DVAFVLVPDRRELLRTDDAGETWSRVG"
     gene            8959..9177
                     /locus_tag="AC150V_05240"
     CDS             8959..9177
                     /locus_tag="AC150V_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762361.1"
                     /translation="MLLFWLVGGLPIILLATLVLALWLTVIELLQLRPNYLWWMWWLI
                     VVFMTHIFGYLALRGYVAYRRRKDSPAT"
     gene            9200..9478
                     /locus_tag="AC150V_05245"
     CDS             9200..9478
                     /locus_tag="AC150V_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762362.1"
                     /translation="MAGLLIAIFLVSRGCQSEGIDLTQEQAIEIARQEIDFEPDLEVV
                     RLIRRGVTFKPHWAVSFSREDEKGNQTDVTVVIIDAETVNVVDVDRSA"
     gene            9702..>10413
                     /locus_tag="AC150V_05250"
     CDS             9702..>10413
                     /locus_tag="AC150V_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3762363.1"
                     /translation="MTATLINLLASAAQDGDELAEAVALDSAILGEFYFFISLPIMWL
                     IHVGFLLYEGGASRRKNVMATAFKNFLTIAVVTPTFYYLGWWIYACFQTGGVSGADGP
                     ENNEGLAGFCGATYPWSEFSGPRLDDHITLVFLLAFIIFSWVTGSIMSGAVLERIRLS
                     AYLLLTALLGSVVWVMAAAWGWSTGWLATNYGYHDAAAAGIVHAVAGFFALGVLVNLG
                     PRIGKYAPGGGVRAFRPHN"
BASE COUNT         1523 a         3622 c         3501 g         1767 t
ORIGIN      
        1 cgatatcctt ccgcgccgtg ggaatcgccc tcggccttgc ggcggcagtg agctggggcg
       61 tcgcagactc gatcgccgcg ctgaccggcc gacgggtcgg ctcgttggcc gtcgtgctcg
      121 gcttccacct ctcggcgtcc gtcttcctcc tcgttctcgt cgccgcgacc ggggcgctcg
      181 gcgacgtcgg gtggagcgac gtagcgttct tcctgctcct cggcgcagtc ggctggatcg
      241 cctatctcgc cttctaccag gctctcgcgc tcggccccgt ctcgatcgtc agccccatct
      301 cgtcgggcta cgcggcagtc acggtcatcc tggcggtgct gatcctggcc gagcggccga
      361 gcggcctcca gtgggtcgcg gtagttgtgg tcttcacggg cgtcgtgttc gcctcgaccg
      421 acctgcgcga gctccggagc ctgcgtgaga tccacgccca gggcatcctg ctcgcggtcg
      481 tcgcgatgct gctgctcggc gccctggttc tcggcgtctc gtcgtcggcg gacgacctgg
      541 gctggctggc gccgatcttc ctcgcgcgcg tgttcgccgc cgccttcctc ggggcaacgg
      601 ccctcgcccg ccgagcgacg ttccgggtgc cccgcgcagc cgggccgctg gcggcgatcg
      661 cactgctcgg cgtgatcgag acgatgggct tcgtgttctt caacatcggc gtggggcacg
      721 ccgacaccgc cgtggtcgcc accgcgtccg cgccctacgc gctcgttccc ctgctcatgg
      781 gcgtcctgct cctgcacgag cgcccgacgc cgacccattg ggtcggcgtc gtgctcatga
      841 tctcaggcgt cgtgctgctc gggctcgcgg cgacctaggc cgctgctccg gctccggcga
      901 atgctggcat gacctgctcg gcgatcagcc gcagctggcc ctggacgtcg ggaacgtcga
      961 cgtccttgcc cgtgaacgcc ttcaggatcg ccgggtcggt gatgcagcag atcatgtggt
     1021 tgacgccggc ctcctcgaag tccttgatcc gtgccgtgca ctcctcgggg gtgccggcga
     1081 tcgagagcgt ctccgcgatc tccgacgtgg tgagctcgta tgccttgtcg aggtcaccgg
     1141 cgccgagggc tgcgacgatc tcgcccatct cctccgggtc gagaccgtgc agcttgagct
     1201 gctcctcggg catcgaggag atgtagaagg ccacgatcga gcgcgccgcc accttcgcct
     1261 tctccgagtc cggcccgcag gtgatcacgc accaggcgcc gatgtcgagc gacgtccagt
     1321 cgcggccggc gcgctcggcg ccgatcttca cgttctcgac cgcgtactcg tacgcctcct
     1381 tcgtatagct gagcgcgacg tggacgccgt ccgtcgcctc gccggcgaac tgcatcgact
     1441 tcggcccgcg catggcgccg atgtacagcg gcatccgctc ctgcaccggt cgggcgaacg
     1501 tgaagagccc actgtatttg tagaactcgc cgtcgtacgt gatcgcgccc tcgtcgagga
     1561 acgtgcgcat cacgtggtgc gcctccttga cgcgcgagaa cggcttgatc ttcgtccagt
     1621 cgacgccgta ctgggagagg agcccgaagt tgccgcacga gatgacgccg tcgacgcggc
     1681 cgccggtcag ttcgtcgagc gtcgcgagtg actggcagat cagcgtcggc tcgcgcaggt
     1741 agacgcccga gacgttgggc ccgaagcgga tggtggtcgt cttgtcggcc gcggcggcga
     1801 agaggagcca aaggtccttg tgccacgtct catcggcggc gtagcaagcg tagtagccga
     1861 gctcgtcggc cgtcttgatc atctccagcg agtcggcgag cgggtagtcg aggagcgtca
     1921 cgtagctgaa cttcatcctt tctcctcctt cttctcggcc tcgatctcgg cgagcagctc
     1981 ctcgccggag acgaccgagt cggcgaagtg gtcgtgcatc caggcgaggt gctcccagcg
     2041 ggagtcgacg atccgaagcg tgtcccaggc ggggatgttc tgctgcagcg tgtgctcggt
     2101 catcagctcg gggtcgacct cgtagacgtg gtcgaagcgc atgacgaccg cctcggtgat
     2161 gcgttgcgcg tcgggcggcc gctgcgccgc caggcgaaag ccgatcatgg ctcgtcctcc
     2221 tcgtaggctt cgtcgagcag gtacttgccc gggttcatga tgttgttcgg gtcgagcgtc
     2281 ctcttgatct tcttcatcac gtcgaagccg ccgcccagct ccagcgggac gagatcgacc
     2341 tcgccctcgc ggcaggcgcc gtggcaggcg gagatggagc cgccgtgccg gatcgcgacc
     2401 tcggcgatgt ctcgcttcgc ctcgacccat gcagcccagc tctcctcgtc ccactgcatc
     2461 tcccagatgc cgatgtcgat ctccgtgagg tagtcggccc acggcttgta ggcgccgttc
     2521 gtgtaggcga acatccccca gtcgtcgaac atctcgaacc gctcgatcag gcggtcggcg
     2581 atggcgtgcc actcctcccg cacctttggc agcgccgagt agggaattgc cgcgtcttcg
     2641 cagtgccagc tcatcggcac cacctggccg ctgcggagcc ggccgtggag cggcgtcgcg
     2701 tagcggtcgt gccgcgcggc ccagtcgccg tgcgagatct cgtcgccgag gtaggtgccc
     2761 ccggtctcga gtccgatctt cagcagccgc ttcgcgcccg cccgcacctc gtcggcgttg
     2821 ccgtacatcg ccgtcgcgat gaccgacttc acttccgggg gctgcgggat gtaggcctcg
     2881 tcgtcgcggc gaaggtagtc gagcttccgc tcgtcgaaca ggaccacgcc ggcgagcgtg
     2941 gcgatgcccg acttcgcgag ggcgcccgtg gactcccagg cggtctcgta gtccgggtac
     3001 cagaagaacg ccgagaactc ggcctcgggg cggggcacca gctcgagtgt cgcttccgtg
     3061 acgatcccga gcgtcccctg gtgccccatg aagaggtgct tgagctggta gccggtcgag
     3121 gactttcgca ccttgcgccc gccgccgtcg ccgacctcga tcagctcccc ggtcggcagc
     3181 accatctgga agctgatcac gaggtcgcgc gtgtgcccgt agcggccgcc gatcagcgac
     3241 cagccgcccg tgccgattcg cccaccgacg agcgagcagg gataggaagc cgggttgtcg
     3301 gggtagatga cgccgtgctg gcgcagctcc tcgttgagct tgagcatgtt gatccccggc
     3361 tgcaccgtca ccgtccggtc gacgaggtcg atgtcgagga tcttgttcat gagcttcacg
     3421 tcgacgacga tcccgtgcct gagcggaacg gcaccgtcgg tgagcccggt gccgcccgcc
     3481 cgcgccacga cgggcagccg gtagcgattc gcgagcttga cgacctcgga gatctgctcg
     3541 gccgaggtcg gcaggacgac gacgtccggg atgtgctcct cccacgcgtg cagagggaac
     3601 ggcgccggca cgcgggcccg gttgtagcgg gccgacaggt tcgtgaagac gtgcgactcg
     3661 ccgcagatct ggttgagctc gcctacgacg tcgtcggaga gagcgaccgt cgtcatctca
     3721 cacctcgaat ccggccgact ccgcgaccag ctccatcaag tcccagacct cgatcgactg
     3781 ctccttcccc tgcgcatcga gcggtctctc ggaccagggg caggccgaga ccagcgtgtc
     3841 gacgccgagt gcggccgcct tctcgacccg cctgcgggag atctcctccg cgatgtcggg
     3901 tcgctcgagg aagaagccag cgccggcccc ggagcagtac gaccactgcg tgacgtggtc
     3961 gacgtccttg aaggtcaggc cgggaattgc ccgcaggatc tcgcgcgggg cttcgtgaac
     4021 gcctttccgc ttgttcagcc ggcaagcgtc gtggtaggtg gccacgcact cgaccgggac
     4081 ggtgagctcg agcttgccct cgcggatcag ctccgccacg agctcgacga cgaggacgat
     4141 ctcgaagtcg tagtcgtcgc cgaacagctc cgggtacatc tccgtgaagg cgatgtagtc
     4201 gtgcgggtcg agacagagga tccgcttcgc tccgacggca cgccagtcgg cgacgttgtg
     4261 ctcagccatg gacgtgaaca gatccgcgta tcccatctca gccgccggcg ccccgcagca
     4321 ccactgctcc ttcatgagcc cgaactcgac gccggccttg cggagaacct gagctaccga
     4381 gcggggaacc gacgtccggt agaacgcggc ctcgcagtcg cagaacagga tcgtctcacc
     4441 gccggtcggg aggtcgagcc cgcgggccca atcgcgcacg ttgttctggc ccacctcgat
     4501 gttccagccg agcaccggct cgttccccca gcgatccgtg agctcgttcc agcgcttcca
     4561 gttcggctgc tcgattccgt tgtcgactgc gagcgtgcgc atcgcccgca cgacgtcgat
     4621 cgtcctagtc cggaagcggt agaagtcgcc cgtgaacagc gtgttggggc agcggatgtc
     4681 gcacgccccg cacatcgtgc agttgacgta gtcctcggcc acgtcctcga tcgacatgtg
     4741 gccccgctcc atcgcgacga cgttcgcgtg gaacgccgtc ggcgtatgtc cctcgtcccg
     4801 cgtgacctgc atcacgggac acacctcgcg gcagaacttg tggccggagc tgaagcagtt
     4861 gaaggcggcg ttgcgccact cgtccaggaa ctcgggtggg agggtgctcc cgccggcccc
     4921 gggcgccttc ccgtcagcca tcgcgctcct ttccgactgc gtagggggat cgtccgcgtc
     4981 gttccacgct cggagatcgt cggcggcgat gctaaacccc tccgatataa gtgtccaggt
     5041 gagcgccagc acgaagccgc tgatcgggct ctcggtcagc ttccacgact tcggcgacta
     5101 tcagggagtc ggcttccagc gtccgatcgc cctggccgga ggagtcccgc tcacgctgcc
     5161 gcggacgatc gagtcgctcg acgacgccct ggaggcgtgc gacggcatcg tcctgggcgg
     5221 tggacgggac atcgagcccg accgctacgg acaggcgccg cacgagacgc tggccgcgac
     5281 cgagccgcac cgtgacgcgt tcgagctcga gctcgtcgaa cgggcgctcg accggggtct
     5341 gccgatcctc ggcatgtgcc gcggcctgca gctgctgaac gtgttcttcg gcggcacgct
     5401 cgtgcaggac gtgagcctcg tgcccgcgtg ggcggagcac ccgtccgacc gtggctgggt
     5461 gcgctggaac gaggtcgagc gggcctcgct gcgaggcgat cccgacgtgc ccgagcaccc
     5521 gcggcatccc atcactgtcg agccgggcag cctcctgcac gacgcgctcg gcgtgaccga
     5581 gatcgaggtg gacagcttcc accaccaggc actcgacgac gtggcgccga atttgagcgt
     5641 ggtcgcgcgc gctccggacg gcgtagtgga ggcggtcgag ctcgattccg gcggctacgt
     5701 gcttgcggtg cagtgggagc tgcaggagga gtggcgcgtc gacccgcgct tcctcgcggt
     5761 gtacgagcgc ttcgtcgcgg ccgccgccgc gcgcaccgag atcatgggct gaagcccagc
     5821 cgggcggaga tctcggcggc atgccggcgg agaggcacgc gggcggcgcg tctggccgca
     5881 gccgtgaagc gcgccgaagg tccctggacg ccgaggatcg ccaccagcgc gccgccggtc
     5941 tcgaacacgg gggccgcgac cgcggtcaga tcgggctcac gttcctctac ggcttccgcc
     6001 cacccccgac gccgcacccg gccgagctcc agctcgagct gctccggatc cgtgaccgtc
     6061 cgctcggtga atccctcgag cggcccctcg ggcaagcgcc ggtcgccgaa agcgagcagc
     6121 accttgccgg tcgccgttgc atgagagacg ctcggccgtc cgagctccgc gacaccctgg
     6181 acgagcgcgg cgctctggac gaagtcgacc gtgacggcgt ccggctcggc gggggcggag
     6241 agcgtggcgg tctcacccgt ctcggcggcg agcgcctcga ggtgtggccg ggcgacctcg
     6301 cgcaggtcga gtcgcgccgc gacggcgttg cccagctgga gcagccgcag cccgagccgg
     6361 tagcggccgg tctcgggaac gtgctcgacg agaccaccgc tcaccagggt cgcgagcagc
     6421 cgtgagaccg tgctcgggcc gatgcccgtg cgacgtgcga gctcgttcgt gccgagcgcg
     6481 ctctcctgcg cggcaagggc ctcgaggaga gcgatcgccc gctcgacgga gccgacttgt
     6541 cgcgccgcag gacgcccggt gcggggcatg ctaggctgag cgtaccaatt tcggcaacac
     6601 tattgcccag gaggcaatga tgtcgggcac cagcttccgc tacgtcgtcg tcggcgcggg
     6661 tatccacgga ctgtcgactg cctaccacct cgccctcgag ctccgctccc gtggcctcgg
     6721 cagcggcgag gacatcgtcg tcctcgagaa gtcacagccg ggagctggcg cgacgggcat
     6781 cgcctgcggc gtcgttcgca acaactactt ccagccggcg atggccgagc tgatgcagca
     6841 ctcggtcgag gtgtgggaat ccgaccctgt cgcgtaccac tacatgccgg tggggtacat
     6901 ggcgctcggc gcagccgtcc aggagggcga cctggtcgcg acgtacgagc gccaggagcg
     6961 gatcggctac agctcccgcc tgatcacagg cgaggacgag gtgtccgagt acatgaaggg
     7021 cctgttcccg gactggcggg cgcgcggggt gaccgcctgc ctgcacgagc tgcagggagg
     7081 cttcgcgttc aactacgagt ccgtgcacgg gctcgccgcg aagtgcggtg ccgaagggat
     7141 cgagatcagg atcggcaacg aggtgacggg cttcggcgag gccggcgacg gctcgatcac
     7201 cgcggtccgc acgaatcggg gccctgtcga ggtgggagag caggtggtgg tcgcgccggg
     7261 cccctgggcg aagtcgttct ggcagatgct cggtctgccg gacgagatcg acatcctcac
     7321 tccggcaggc gagcggctcg agggtcagcc gatgtggacg tactggaacc tccaggaggg
     7381 cgagatcgcg gtcgacccgc tcatgttcga gacggtcgac ggcggggcgc cgccggtaat
     7441 ccacctcgac tcggatgccc cgctcaacgc cgacgacgga acgctcgtca cggacgagct
     7501 ctggggcgtc tacttcaagc gtgaccgcca cggcgtgcag ggcggcgcct ctccgctcgt
     7561 cgtcgacggc gacatcgagc tcgacccgta cccgcagacg acggacgttg acgccggctt
     7621 cccggacatg tggtgcgcgg cgctgtcaca tgcgatggcg cgcttcgaag ggtgccggcc
     7681 gcagtacatg caggcccgct ccggcggcgt gggcgccttc acggcggaca acttcccggt
     7741 cttcgactac ctgcggccga acctctacgc gatcctcgac tcgaaccacg gctacaagat
     7801 gatcggggtg ggcaaggagg tggcgcgggt ggtcaccggc gagcactcga gcctcctgca
     7861 cccgttccgc ttcgagcgct tcgcgacggg cgacctccat ccggtctcga gctctcccta
     7921 cccctggtcg taggcggctg caacagtctg ttacttgccc tccctccgct cacccagacc
     7981 gaaaacgcca agcaagcaac agactgttac ttgcttacct atgcgtcagc cgacgcgcga
     8041 ccacgtctcg ccggcgtcgt ccgtgcgaag gagctcgcgc ctgtcgggga cgagcacgaa
     8101 agcgacatcg ggtgaccgcg gcgaccaggc ggccgcgccg gccggagctc cggggtcgag
     8161 cgattcggcc cacgtcgtcc ccccgtcagt cgagatccag accccgtcac ccgctgcgag
     8221 gacgtggtcg ggatccgctc cgctcgccgc aatgctccgc acccgctcgg agttgacgat
     8281 ttcccaggtg cggccaccgt cctcgctccg caggacacct cgaccgggcg cgaccgcgac
     8341 gagcgaatca cccgctgcgg caagcgcggt caccccgtct gctccggacg ccgcgagcgg
     8401 ctcgaagctc tcccccgcgt cggcggagac gaacagccct tccttcgtcg cggcgtagag
     8461 ccgtccgtcc gccgcggcgg cgagcccggt aagaccgccg gccgcgagcc ctgaggctgc
     8521 gtgctcgacc cagctggcgc cgccgtcgct cgagtgcgcc acgatatcgg gcccggcggc
     8581 ccagagtgag ccgtcgggag agtgggtgag cacgaggacg ttctcgccgt cgaggcgtcc
     8641 gagctcccac tctttcccgc cgtccgcgga cacgtagagg cctacctgcg ttccgaggac
     8701 aaggctcgcg tccgcgccct ggccgacagc gagcgcgctc gaatccagcg cgctcggcag
     8761 gccttcgatc ggcgggacgt gcccgctagt cgcctggtgt cgagcgaaga ggaaggcgcc
     8821 gacggccgcc gcgacgatga gcgcaggcca gacgaggcgc cgcaaccagc gcccggctcc
     8881 gggagcactc gctgccggaa cccgctcggc cgccgctggc ttcttgaacc gcgccacgaa
     8941 gtgcctcagt atggcccaat gcttctgttc tggctcgtcg gcggcctccc gatcatcctc
     9001 ttggcgactc tcgtcctcgc cctgtggctc accgtcatcg agttgctaca gctccggccg
     9061 aactacctct ggtggatgtg gtggttgatc gtcgtcttca tgacgcacat tttcggctac
     9121 ctggccctgc ggggatacgt tgcctacaga cgccgaaaag actcgcccgc tacttaaacc
     9181 cggcgtcgcc ctgctcttca tggcggggct gctgatcgcg attttcctcg tctcgcgcgg
     9241 ctgccagtcg gagggcatcg acctcaccca ggagcaagcg atcgaaatcg cgcgtcagga
     9301 gatcgacttc gagccggacc tcgaggtcgt gcgcctgatc cggcgtggtg tcaccttcaa
     9361 gccccactgg gcggtgtcct tctcccgcga ggacgagaag ggaaaccaga ccgacgtcac
     9421 cgtcgtgatc atcgacgccg agaccgtcaa cgtggtcgac gtcgaccgaa gcgcctgacg
     9481 acccgccggt gtggcacctc cacaccgtca ggcgacaacc gtggagcaac cgcacatagc
     9541 tgcggcctcg ccgagcggtc tagggtagcc cgttcgccga tggagagcag gcagccagcc
     9601 agcatgcggt ccgacgtcgt gtcaggttcc aagggttgta accgtccgca cgacgtgtta
     9661 gcttcgctgc cgcttagcga ccccaacgga gggagtgcgt cgtgacagcc acgttgatca
     9721 acctgctcgc ttccgccgca caggatggtg acgagctggc ggaggcagtc gccctcgact
     9781 cggcaatact gggcgagttc tacttcttca tctcgctgcc gatcatgtgg ctgatccacg
     9841 tcggcttcct tctctacgag ggtggtgcgt cacggcgcaa gaacgtgatg gccacggcgt
     9901 tcaagaactt cctcaccatc gctgtcgtca cgccgacgtt ctactacctc ggctggtgga
     9961 tctacgcctg cttccagacc ggaggagtct cgggagcgga cggcccggag aacaacgaag
    10021 ggctggccgg cttctgcggc gccacctatc cgtggagcga gttcagcggc ccacgcctcg
    10081 acgaccacat cacgctggtc ttcctcctgg cgttcatcat cttctcctgg gtcacgggct
    10141 cgatcatgtc gggcgcggtg ctggagcgca ttcggctctc cgcctatctc ctcctcaccg
    10201 cattgctcgg ctcggtcgtc tgggtcatgg ccgccgcttg gggctggagc acgggctggc
    10261 tcgcgaccaa ctacggctac cacgacgcgg cggcggccgg catcgtccac gccgtggcgg
    10321 ggttcttcgc gctcggcgtg ttggtcaacc taggcccgcg tatcggtaag tacgcccctg
    10381 gtgggggggt gagagccttc agaccccaca acc
//
LOCUS       DBNGNK010000180         1887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_389298,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000180 DBNGNK010000000
VERSION     DBNGNK010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_389298"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="AC150V_05255"
     CDS             <1..833
                     /locus_tag="AC150V_05255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3762364.1"
                     /translation="FHGFRRDDLHETQSVTKSVTSLVVGIARDQGAIDDLATPVLEWF
                     PEFAATAQPGWEKVTVEHLLTMTAGLDWDQREVLQAWPGGPTLFAEIFSRRVVHEPGS
                     RWLYNGADIGLLGGIVRRATEMQVDELAARTLFGPLGITAWDWERGKRDGYPSLAGAL
                     HLRPLDMARIGQMVLDGGRWQERQVVSEEWIAESTAPQVVPNVESQRYGYFWVRLGAQ
                     LDAGPCPVLVASGWGSQFIHIVPALDAVIVTTGGNHFNGKTFAVGEVLLRELVPGVER
                     "
     gene            complement(845..1582)
                     /locus_tag="AC150V_05260"
     CDS             complement(845..1582)
                     /locus_tag="AC150V_05260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR3762365.1"
                     /translation="MRALIVDDEAVARRRLGRLLAAHDDLEVVGEASDGKAALERIEE
                     LEPDVVFLDIRMPEIDGLELARQLDDVQVVFTTAYDEYAVEAFETSAVAYLLKPIEKK
                     RLAAAIEKVRARQARSSEHNLEHLLRRIARRNEPPRITARRGDAIRVFDPREITRFHA
                     QDGYTGFRHEGRVFLLDDSITALAERLADWGFVRIHRGELVNLSHIRALRREDDQSLV
                     ELADGQRAPVSRRHLKELKQRLGIPVR"
     gene            complement(1579..>1887)
                     /locus_tag="AC150V_05265"
     CDS             complement(1579..>1887)
                     /locus_tag="AC150V_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3762366.1"
                     /translation="QPLVENAVLHAIAPRSGGGSLTVSAGRNGDSLRLEVVDDGPGLG
                     LSRHRGSGTSLEDLARRLEMIYGGRARLDVSPADDAGCRVTLSLPLGASEDVGGAEGT
                     "
BASE COUNT          279 a          598 c          693 g          317 t
ORIGIN      
        1 acttccatgg tttccggcgg gacgacctgc acgagacgca gtcggtgacc aagagcgtga
       61 cctcgctggt ggtcgggatc gcccgcgatc aaggggcgat cgatgatctg gcgacgccgg
      121 tgctcgagtg gttccccgag tttgcggcca ctgcgcagcc gggatgggag aaggtgacgg
      181 tcgagcacct gctgacaatg acggcgggct tggactggga ccagcgggag gtcctacagg
      241 cgtggccggg aggaccgacg ctgtttgcag agatcttcag ccgccgggtg gtgcacgagc
      301 ccgggagccg ctggctctac aacggcgcgg acatcggact tctgggcggg attgtgcgtc
      361 gcgccaccga gatgcaggtc gacgagctgg cggctcggac gctcttcggg ccgctcggga
      421 tcacggcctg ggactgggag cgaggcaagc gcgacggcta tccctcgctt gccggggcgc
      481 tccacctgcg gccgttggac atggcgagga tcggtcagat ggtgctcgac gggggacgct
      541 ggcaggagcg gcaggtggtc tcggaggagt ggattgccga gtcgacagca ccacaggtgg
      601 ttccgaacgt ggagtctcag agatacgggt acttctgggt gcgtctcggc gcgcagctcg
      661 atgccggtcc ctgtccggtg ctcgtcgcct cgggctgggg cagccagttc atccacatcg
      721 tcccggcgct cgatgcggtg atcgtgacga ccggcggaaa ccacttcaac ggcaagacgt
      781 tcgcggtcgg cgaagtgctg ctgcgggagc tggttccggg ggtcgagagg taggtcgcac
      841 cggcctaccg aaccggaata cccaggcgct gtttcagctc tttcagatgt cggcggctga
      901 ccggcgcccg ctggccgtcg gcgagctcga cgaggctttg gtcgtcttcg cggcgcaagg
      961 cccggatgtg ggagaggttt acgagctcac cccggtgaat ccggacgaag ccccagtcgg
     1021 ccagccgttc ggccagcgcg gtgatggagt cgtcgagaag gaagactcgg ccctcgtgcc
     1081 ggaatccggt gtagccgtcc tgggcgtgga agcgcgtgat ctctcgcgga tcgaagaccc
     1141 gaatcgcgtc accgcgccgc gcggtgatgc gcggcggctc gttgcggcga gcgatacgac
     1201 gaagcaggtg ctcgagattg tgctcgctcg accgagcttg ccgggcccgg accttctcga
     1261 tggcggccgc cagcctcttc ttctcgatcg gcttcaagag gtacgcgacg gccgaggtct
     1321 cgaacgcttc gaccgcgtat tcgtcgtagg cggtggtgaa gacgacctgc acgtcgtcga
     1381 gctgccgggc gagctcgagg ccgtcgatct cgggcatgcg gatgtcgaga aagaccacgt
     1441 cgggctcgag ctcctcgatt cgctcgagcg ccgctttacc gtcgctggcc tcgccgacga
     1501 cctccagatc gtcgtgcgcg gcgagcagcc gcccgagacg cctgcgcgcg accgcttcgt
     1561 cgtcgactat cagcgctctc acgtaccctc cgcgccgccg acgtcctcgc tggcgccgag
     1621 cggcaggctg agcgtgaccc ggcaaccggc gtcgtccgct gggctcacgt cgagccttgc
     1681 ccggccgccg tagatcattt ccagtcgccg ggcgaggtcc tcgagcgacg tgccgctgcc
     1741 ccggtggcgc gagaggccga ggccggggcc gtcgtcgacc acctccaggc gaagggaatc
     1801 tccgttgcgt ccagcgctga cggtgaggct gccgccgccg gatcgcggcg cgatggcgtg
     1861 gagcaccgcg ttctcgacga gcggctg
//
LOCUS       DBNGNK010000181         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1035039,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000181 DBNGNK010000000
VERSION     DBNGNK010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1035039"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..995
                     /locus_tag="AC150V_05270"
     CDS             150..995
                     /locus_tag="AC150V_05270"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR3762367.1"
                     /translation="MLISFRELLQERRACGAAAGAITCYDLTTALGVLRAAEARDVPV
                     VLLVSDASFRGPDGRPLVAALLAVAAQARVPACVQLDHATDATVLSAALAAGVGAVMA
                     DGSKLSTAENAAFVRDVRREALGAGAGVEAELGHIEGGEDVAAAVAAGALTDPDEAAA
                     FVQDTEIDCLAVSIGNVHGAYAAPPELDWERLERIRSRVGDVPLSLHGASGLPADDVR
                     RAIALGVCKVNVNTEIRGRYLEELDRRLPHARAGSRLLELESAVVAAVADTVGAKLDL
                     FDRRR"
     gene            1022..1918
                     /locus_tag="AC150V_05275"
     CDS             1022..1918
                     /locus_tag="AC150V_05275"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HIR3762368.1"
                     /translation="MDTRQLAAFCAVVERRSFSRAAEELGVTQPAVSLQVQALEKRLG
                     TQLIDRSGRSVEPTEAGRKLFDGARKLLALEHEVLEGVAAEAGAELSGVLAIGASTGP
                     AHVILPLLLCEFQSQHPAVRIRFEVHDTQTVVELVAERQLELGIVGAARRRRGVRYEP
                     FMRDEVILVCPPGHPFAGRTVTLEELRRQPPILMQEGAGVRQVLEDELRRLGTRLRDL
                     DAPLQLGLQESVRSAVLAGHGVAFVSRRAVEDDLAAGRVDEARVEGMSGGREISLARP
                     TGHTLTRVADAFAGFSRDRIAW"
     gene            2086..3126
                     /locus_tag="AC150V_05280"
     CDS             2086..3126
                     /locus_tag="AC150V_05280"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3762369.1"
                     /translation="MTSPTEPRPGRVVRFGAGTLELLEELAGELGLKRLLLVTTQRGS
                     AVGAGLPVESVYDGVRSHVPIETVVEGALRARAAAADGLVALGGGSAIDTCKAMTVEL
                     LPDHEVRTIAVPTTYAGAEWTPFFGITLEPGRKSGGSEERARPIAAIYDPELQLDLPP
                     VPTVGTAMNALAHCAEAYYAPGRSETSDADADKGARSIAEALPLVVAEPGSLDARSLL
                     LEGAMHAAFALGDAGLGLAHAMAQALGGRYGLPHGAMNALCLPPALRFNEPAVPDAVA
                     RFGNAVGAAGPAAAVEALARLGGFERLRDFDIPEQELGLVGEAAAARPGARANPRPAG
                     PPEVTALLREIW"
     gene            3166..>3350
                     /locus_tag="AC150V_05285"
     CDS             3166..>3350
                     /locus_tag="AC150V_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762370.1"
                     /translation="MSETPAQPKPPAGRWRRIAARVLTVIGILLAIIALHANWVRTQL
                     LDTDEFKETAALLIESEP"
BASE COUNT          422 a         1201 c         1243 g          484 t
ORIGIN      
        1 gaggtcgtcg gccccgtcct cccgggggtc tcgcggtggc gggtcgcggc cgacggcggc
       61 gagatcgact acctggtcgc tcccggcaac gtgggtgacg acgagctgct cgtggagctc
      121 gtcgagctcg tgctgagagg atcacgcgcg tgctgatctc tttccgggag ctcctccagg
      181 agcgacgggc gtgcggcgcg gccgcgggcg cgatcacgtg ctacgacctc acgaccgctc
      241 ttggcgtgct ccgggccgcc gaggcccgcg acgtgccggt cgtgctgctc gtgagcgatg
      301 cgtcgttccg cggccccgac ggtcggcccc tggtcgccgc gctccttgcg gtcgccgcgc
      361 aggctcgcgt ccccgcgtgc gtccagctcg atcacgcgac cgacgccacg gttctctcgg
      421 cggcgctggc ggccggcgtc ggtgcggtca tggccgacgg ctccaagctg tccacggccg
      481 agaacgccgc cttcgtccgg gacgtgcggc gcgaggcact tggggccggg gcgggcgtag
      541 aggcggagct cggccacatc gagggaggtg aggatgtcgc cgcggcggtc gcggcgggcg
      601 cgctcaccga ccccgacgag gcggcggcgt tcgtccagga caccgagatc gactgcctcg
      661 ccgtctcgat cggaaacgtc cacggcgcct acgccgctcc cccggagctc gactgggagc
      721 ggctcgagcg catccggtca cgggtgggag acgtgcccct gtcactccac ggggcctccg
      781 ggctccccgc cgacgacgtc cgccgcgcga tcgcgctcgg cgtctgcaag gtgaacgtga
      841 acaccgagat ccgcggccgc tacctcgaag agctcgaccg caggctgccc cacgcgcgcg
      901 ccgggtcgcg tttgctcgag ctcgagtcag cggtcgttgc ggccgtcgcc gacaccgtcg
      961 gcgcgaagct cgacctcttc gaccgcagga gatgactcgc gccttcggat aagttctgct
     1021 tatggacacg cgtcagctgg ccgcgttctg cgccgtcgtc gagcgccgga gcttctcgcg
     1081 ggccgcggaa gagctgggtg tgacgcagcc ggccgtgtcg ctccaggtgc aggcgctcga
     1141 gaagcgccta ggcacgcagc tgatcgatcg gagcggccgc agcgtcgagc cgaccgaggc
     1201 cggacgcaag ctgttcgacg gcgctcggaa actgctcgcg cttgagcacg aggtgctcga
     1261 aggcgtcgcg gccgaggccg gcgccgagct ttcgggcgtg cttgcgatcg gcgcttccac
     1321 tggaccggcg cacgtcatcc ttcccctcct gctatgcgag ttccagtccc agcatcccgc
     1381 ggtgcgcatc cgattcgaag tccacgacac gcagactgtc gtcgagctcg tcgccgagcg
     1441 ccaactcgag ctcggcatcg tcggcgccgc caggcggcgt cgcggtgtgc ggtacgagcc
     1501 gttcatgcgg gacgaggtga tcctcgtatg cccgcccggg catcccttcg cggggcgaac
     1561 cgtgacgctc gaggagctgc gccggcagcc gccgatcctg atgcaggaag gagcaggggt
     1621 gcggcaggtg ctcgaagacg agctccggcg actcgggacg aggcttcggg atctcgacgc
     1681 gccgctgcag ctcggtctgc aggagtcagt ccgcagcgct gtgctcgcgg ggcacggggt
     1741 cgctttcgtc tcgcgccgtg ccgtcgagga cgacctcgct gcggggcgcg tggacgaggc
     1801 gcgggtggag gggatgagcg gcggccgcga gatctcgctc gcacggccca ccgggcatac
     1861 gctgacgcgg gtggccgacg ccttcgccgg cttctcgcgc gaccgcatcg cctggtaggc
     1921 gagcctttta ctgcgcctcc gactcaccga gtcggctctg taggctccgg gcgtgtctcg
     1981 gccgcgcgac tcctgcggcc ggcgtgtcga gctgcctcct ggtcgcggcc gccggctttc
     2041 tcaggctcga cctgtcacgg cggtcggagc ggcggcggcg acgccgtgac gagtccaacc
     2101 gagccgcgac cgggacgtgt cgtgcgcttc ggcgccggca cgctcgagct gctcgaagag
     2161 ctggccggag agctcgggct gaaacggctg ctgcttgtca ccacgcagcg cggctcggcg
     2221 gtcggagccg gcctgccggt cgagtcggtg tacgacggcg tccgctccca cgtcccgatc
     2281 gagacggtcg tcgagggcgc cctgcgtgcc cgcgccgcgg ctgcggacgg cctcgtcgcg
     2341 ctcggcggcg gcagtgcaat cgacacgtgc aaggcgatga cggtcgagct gctccccgac
     2401 cacgaggtgc gcacgatcgc cgtgccgacg acctacgccg gcgccgagtg gacgccgttc
     2461 ttcggcataa cgctcgagcc cggtcgtaag agcgggggct ccgaggagcg tgcccgcccg
     2521 atagcagcca tctacgaccc ggagctccag ctcgacctgc caccggtgcc gacggtcggg
     2581 acggcgatga acgcgctcgc ccactgcgcc gaggcctact acgcgccggg ccgttccgag
     2641 acgagcgacg cggacgcgga caagggcgcc cgctcgatcg ccgaggcgct gccgctggtc
     2701 gtcgccgagc cgggatcgct ggacgcccgg tcgctcctgc tcgagggggc gatgcacgcc
     2761 gcgttcgcgc tcggcgatgc gggcctcggc ctcgcccacg cgatggcgca ggcgctcggc
     2821 ggccgctacg gacttcccca cggtgcgatg aacgcgctct gcctgccgcc ggcgctgcgt
     2881 ttcaacgagc ccgccgtgcc ggacgcggtg gcccgcttcg ggaacgccgt cggagcagct
     2941 ggcccggctg cggccgtcga ggcgctcgcg cggctcgggg gcttcgagcg gctgcgcgac
     3001 ttcgacatcc ccgagcagga gctcggcctc gtcggcgagg ctgcggcagc gcgcccgggc
     3061 gcccgggcga acccgcggcc ggcgggacct cccgaggtca cggccttgct ccgcgagatc
     3121 tggtgaccga gcccgcgcgg ataccgactc gctaggctgg cgcgagtgag cgagaccccc
     3181 gctcagccca agccgccggc agggcgctgg cgccgcatcg cggcccgcgt cctgacggtg
     3241 atcgggatcc tgctggcgat catcgcgctg cacgcgaact gggtccgcac gcagctgctc
     3301 gacaccgacg agttcaagga gacggcggcg ctgctgatcg agagcgagcc
//
LOCUS       DBNGNK010000182         4429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1751646,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000182 DBNGNK010000000
VERSION     DBNGNK010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1751646"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="AC150V_05290"
     CDS             <1..1807
                     /locus_tag="AC150V_05290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3762371.1"
                     /translation="NVRSAGILGGMHRLSVSMRWWLALVFAAMVAMTAVVVAQVLAVR
                     SDRAFRERAEELAAGAAVTAANAITNNAEDQTLAESAAAAARARRIAIFLFDEDGTLL
                     TPSTSNGVALGSLANLDDALVAGSERRRLVESVNDGRRIVVSLPLAAADATTLVAVAA
                     RPDLVAAGDIVRGELPATIAIAIGVGALAGTAVAILITRRIRRIAQAAAEIEQGSFDE
                     PLEPRFDDELGQLAATVDSMRLHLRDSFHQLGSERDRLRSLFEQLQEGVIAVDPELRV
                     VFANSRSRLIFGSRALAEGVDLPDPWGDGTLREVAAGLFRPGATVVNVRVSPDPERTY
                     AVAGVPLEEGGDTGLLVLADITVADRRERAEREFVANAAHELRTPLAAIASAVDVLQA
                     GAKDDAAERDRFLSVIDRQSARLGRLVRSLLTLARAQTRAEPIVLERVEGRPLLLEIA
                     AELGDRSDVHVEARCDPSLEAHAHHDLLAQAVENLAANAVRHAAAESVLLTAVRVPGG
                     RVRIDVRDNGSGISEGERDRLFDRFYQAGERDPSGFGLGLPLVRAAVTALGGEVELES
                     SEGRGTTASIYVRAGDSPTRASTLPATTVARDGLR"
     gene            1804..2490
                     /locus_tag="AC150V_05295"
     CDS             1804..2490
                     /locus_tag="AC150V_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005286999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3762372.1"
                     /translation="MSTRILLVDDERDITEPVAYALEREGFEVECREDGEAALSTVRG
                     EAFDLVILDVMLPRVSGMDVCRTLRSESDVPIIMLTARDAEVDRVLGLELGADDYVTK
                     PFSTAELISRVRALIRRRELDRTSTARNAREVGGISIDLARHSVAVDGTPVRLTRSEF
                     RVLTLLAEEPGRVFSRRQIMEHLWESDYVGDARAADVHVSNLRRKIERDPAHPERIVT
                     VREVGYKLVA"
     gene            2690..3094
                     /locus_tag="AC150V_05300"
     CDS             2690..3094
                     /locus_tag="AC150V_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3762373.1"
                     /translation="MSATSPATTTGAPLATREIGTGLWLLVPRGDLDGDMVPALRSAC
                     VAALDRGGLDIVVDLRGVRAMCLDALDILALVSETLLARGGFLWLAHEVQRSCDLVSV
                     DERGLEAVEGISRALDDALRADRIVSEVAPGA"
     gene            3087..4337
                     /locus_tag="AC150V_05305"
     CDS             3087..4337
                     /locus_tag="AC150V_05305"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF016032.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR3762374.1"
                     /translation="MRSALGRQPTGRAQPRPKPALGAAEGVATRHARAVFDRTTAFTV
                     GAEEEFFLVDPVTLEPAPLGELALVVSEGDARVSSEFRACQVEATSPVCLSVADVARE
                     LRSVRTLLHDRLAGQALIVAAGTHPFAFDPGPISDGPRYARIAAENPWAARFKLTCGL
                     HVHVAVGSSETTLAVHNALRSYLPELTALTANAPFFRGEDTGLASVRPRLNQALPRHG
                     VPPAYASWDEWGQHVEWTSRSALYPDTTHHWWDLRLHPGYGTIELRAADVQTRIEDSA
                     TVIALVQSLVAFLARRAEAGEEIHRHDTQRINESVWSAARDGVSGVLPDLESGKRTPT
                     VERIRRLVEGLLPTAVELGCGAELLRVEHMLVDDGATRQRRVAAERGHDGLVAWLAEQ
                     TVGTDSFFPEFDPPRLRSLESGAQ"
BASE COUNT          628 a         1570 c         1550 g          681 t
ORIGIN      
        1 aaacgtccgc tcggcgggaa tacttggagg gatgcaccga ctctcggtga gcatgcgatg
       61 gtggctggcg ctggtcttcg ccgccatggt cgccatgacc gcagtcgtcg tggcccaggt
      121 gctcgccgtg cgctcggacc gcgcgtttcg cgagcgcgcc gaggagctcg cggcgggtgc
      181 cgcggtgacg gctgccaacg cgatcacgaa caacgccgaa gaccagacac tggcggaatc
      241 ggccgctgcc gcggcccggg cgcggcgcat tgccatcttt ctcttcgacg aggacggcac
      301 gctgctgacc ccctcgacgt cgaacggtgt cgcgctcggt tcgctcgcga acctcgacga
      361 cgcgctggtg gcggggtcgg agcgccggcg cctcgtcgag agcgtgaacg acggtcgccg
      421 gatcgtcgtc tcgctgcccc tggccgcggc cgacgccacg acgctcgtgg cggtggccgc
      481 gcgacctgat ctcgtcgccg cgggcgacat cgtccgcggt gagctcccgg cgacgatcgc
      541 catcgccatc ggcgtcggcg ccctcgcggg tacggccgtg gcgatcctga tcacgagacg
      601 aatccgtcgc atcgcgcagg ccgccgcgga gatcgagcag ggaagcttcg acgagccgct
      661 cgagccgcgg ttcgacgacg agctcggcca gctcgcggcg accgtggaca gcatgcgcct
      721 ccatctgcgc gactcttttc accagctggg ctccgagcgt gaccggctcc gctcactgtt
      781 cgaacagctc caggagggtg tgatcgccgt cgatccggag ctccgcgtcg tctttgccaa
      841 ctcgcggtca cggctcatct tcggcagccg tgcgcttgcg gagggggtcg atctccccga
      901 tccgtggggc gacggaacac ttcgcgaggt ggccgccggc ctcttccgcc cgggtgcgac
      961 cgtcgtgaac gtccgcgtct cgccggatcc agagcgaacg tacgccgtcg ccggggtgcc
     1021 gctggaagag ggcggcgaca cgggcctgct cgtgctcgcc gacatcacgg tcgccgatcg
     1081 gcgcgagcgg gcggagcgtg agttcgtcgc caacgccgcg cacgagttgc gcacgccgct
     1141 ggcggcaatc gcgagcgcgg tcgacgttct gcaggcgggt gccaaagacg acgcggccga
     1201 gcgcgaccgc ttcctctccg tgatcgaccg ccagtcggcg cggctgggac gactagtgcg
     1261 ctcgctgctg acgctcgcgc gggcgcagac gagagccgag ccgatcgtgc tcgagcgggt
     1321 cgagggtcgg ccgttgctgc tcgagatagc agccgagctc ggcgacagga gcgatgtcca
     1381 cgtcgaagca cggtgcgatc cgtccctcga ggcccatgcc catcacgacc tgctcgcgca
     1441 ggcggtcgag aacctcgctg ccaatgccgt gcgtcacgcc gcggccgaat cggttctgct
     1501 gacggcagtc cgcgtcccgg gcggtcgcgt ccgcatcgac gtgcgcgaca acgggagcgg
     1561 gatctccgag ggcgagcgcg atcggctctt cgaccgcttc tatcaggcag gcgagcgcga
     1621 cccgagcggg ttcggcctcg ggctgccgct cgtccgtgcc gcggtgacgg cgctgggcgg
     1681 agaggtcgag ctcgagtcct cggaagggcg aggaacgacg gcgtcgatct atgtccgggc
     1741 cggcgactcg ccgactcgag cgagcaccct gccggcgact accgtggcgc gggacggact
     1801 ccgatgagca cgcggatcct gctggtcgac gacgagcgcg acatcaccga gcccgtcgcg
     1861 tacgcgctcg agcgggaggg tttcgaggtc gagtgccggg aggacggcga ggccgccctc
     1921 tcgacagtcc gaggagaggc gttcgacctc gtgatcctcg acgtcatgct gccccgcgtc
     1981 tcgggcatgg acgtctgtcg gacgctgcgc tccgagagtg acgtgccgat catcatgctc
     2041 accgcgcgcg acgcggaagt cgaccgcgtc ctcgggctcg agctcggcgc cgacgactac
     2101 gtgacgaagc cgttctcgac cgcggagctg atcagccgcg tgcgcgcgct gatccggcga
     2161 cgcgagctcg atcggacgag cacggcgcga aacgcgcgag aggtcggcgg catctcgatc
     2221 gacctcgcgc ggcactccgt ggcggtcgac ggaacgcctg tgcggctgac gcgctccgag
     2281 ttccgcgtgc tcaccctgct cgccgaggag cccgggcgcg tcttctcccg ccgccagatc
     2341 atggagcatc tctgggagag cgactacgtc ggtgacgcgc gcgccgccga cgtgcacgta
     2401 tcgaacctgc ggcggaagat cgagcgcgac ccggcacatc ccgagcggat cgtgaccgtc
     2461 cgcgaggtcg gctacaagct cgtcgcctag cccgactgcc cccgtcgctc cgttgcgatt
     2521 cgcgggcgtc gttaaccgtc gcgcaaccgt gtgaggaaag gctcacgaat tcaaaagacc
     2581 ctgctcacag gggtggaatg gaggttcgca ctgggcccct acggtgtgag gagcatcgac
     2641 cgaacgccgt ttgggggctg acgctcccgt cgcctatccg aggagccgaa tgagcgcaac
     2701 gagtccagcc accaccacag gagccccgct cgccacgcgc gagatcggga cggggctctg
     2761 gctgctcgtc ccgcgcggcg acctcgacgg agacatggtg cccgcgctgc ggagcgcctg
     2821 cgtcgccgca ctcgatcgcg gcgggctcga catcgtcgtc gacctgcgcg gcgttcgggc
     2881 gatgtgcctc gatgccctcg acatcctcgc tctggtctcc gagacgcttc tcgcccgcgg
     2941 cgggttcctc tggctcgcgc acgaggttca gcgctcgtgc gacctcgttt cggtcgacga
     3001 gcgaggcctc gaggccgtcg agggcatcag ccgggctctc gacgacgctc tgcgtgccga
     3061 ccgaatcgtc tccgaggtcg cgcccggtgc gtagcgcgct cggtcgacag cccaccggtc
     3121 gggcgcagcc caggccgaag cccgcgctgg gcgcggcgga gggtgtcgcg acccgtcacg
     3181 ctcgtgcggt cttcgatcgc accaccgctt tcaccgtcgg ggctgaggag gagttcttcc
     3241 tcgtcgaccc ggtcacgctg gagccggcgc ctctcggcga gctggcgctg gtcgtctcgg
     3301 aaggagatgc gcgtgtcagc tcggagttcc gcgcctgcca agtcgaagcg acgagtccgg
     3361 tctgtctctc ggtcgccgac gttgcccggg agctgcggtc cgtgcggacg ctcctccacg
     3421 accggctcgc cggtcaggcg ttgatcgtgg ctgcaggcac ccatccattc gcattcgatc
     3481 cgggcccgat cagtgacggg ccgcgttacg cacgcattgc cgccgagaac ccgtgggcgg
     3541 cacgcttcaa gctcacctgc gggctccacg tccacgtcgc cgtgggtagc agcgagacga
     3601 ccctggccgt ccacaacgct cttcgctcgt acctcccgga gttgacggcg ctcaccgcga
     3661 acgccccctt cttccgtggt gaggacaccg gtctggcgag cgttcgccca cgtctcaacc
     3721 aggcgctccc gcgacacggc gtgccgccgg cttacgcgag ctgggacgaa tggggccagc
     3781 acgtcgaatg gacgtcccga agcgcgctct accccgacac gactcatcac tggtgggatc
     3841 ttcgcctcca cccgggctac gggacgatcg agctccgcgc cgccgacgtg cagacacgga
     3901 tcgaggactc cgcgacggtg atcgcgctcg tccagagcct cgtcgcgttc ctcgccaggc
     3961 gggccgaggc cggtgaggag attcaccgcc acgacacgca gcgcatcaac gagtcggtgt
     4021 ggtcggctgc tcgcgatggc gtttccggcg tgctcccaga cctggagagc ggcaagcgga
     4081 cgcctaccgt cgagcgcatc aggcgtttgg tcgaaggact gctgccgact gcggtcgagc
     4141 tcggctgcgg cgccgagctc ctgcgcgtcg agcacatgct cgtcgacgat ggagcgactc
     4201 gtcagcgccg ggtcgcggcg gaacgaggcc acgacgggct cgtcgcctgg ctcgccgagc
     4261 agactgtcgg caccgactct ttcttccccg agttcgaccc ccctcgactc cgctccctcg
     4321 aatccggggc ccaataggcg ggggccccgg ttccagcccg gcgcccgccc ccgcgggcgc
     4381 cgggtagcca gcccgccctg gtcctacggc ggctagccga agcccgcac
//
LOCUS       DBNGNK010000183         3612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1248989,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000183 DBNGNK010000000
VERSION     DBNGNK010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1248989"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="AC150V_05310"
     CDS             complement(<1..1105)
                     /locus_tag="AC150V_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HIR3762375.1"
                     /translation="MPLDEYRRKRDSARTPEPFSGGESGADARFVVQRHDARRLHYDF
                     RLERDGVLASWAVPKGIPTRKGERRLAVQTEDHPLDYADFEGTIPAGQYGAGTVEIWD
                     RGTYALVEQKRDGGFTVRLEGRRLAGVWTLVAASLDGDPRNWLLICKDGVAQQRATYE
                     PMLASSAEVVPSGEGWVFEPKWDGFRAIIAVDGGKVALTSRNGNDLTERFPTVAREIP
                     RAVRASSAVLDGEICALDEAGRSQFGLLQQGAGTLVLALFDALELDDETLVDLPLAER
                     RARLEAAVVPGRPTVFVSPSFEDGHALLDAAAGEGFEGIVAKRASSRYIPGRRTNDWR
                     KLKVRRRQEFVIAGYTRVAAGGAPASGRSYSVCA"
     gene            complement(1235..2182)
                     /locus_tag="AC150V_05315"
     CDS             complement(1235..2182)
                     /locus_tag="AC150V_05315"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR3762376.1"
                     /translation="MTREAELRLRDRLSRLGKVKLLRGTKVSGELERLQRQLEHLQAD
                     ATDEEVWRSVELARHQDRPYTLDYVERILDDCFELHGDRGGMDDEAIVAALGKLDGVT
                     IAVVGHQKGRDIKERASRNFGMAYPQGYRKAMRTMALAQRHGFPLLTLVDTPGAYPGV
                     AAEQAGQGGAIARSQAVMVRLEVPTVSVVIGEGGSGGAVAIAVADRVLMQENAIYSVI
                     SPEGCAAILWRDAAEARKAASAFKPDAAHCLELGVIDAVVPEPPGGAHLDHDAAAEFL
                     RASVVAAFSDLDGVSTDDLLRRRRAKYRAMGVLAQAPAL"
     gene            complement(2179..3024)
                     /locus_tag="AC150V_05320"
     CDS             complement(2179..3024)
                     /locus_tag="AC150V_05320"
                     /EC_number="2.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007102365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:2001295 - malonyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HIR3762377.1"
                     /translation="MAAADRIDVSIEHKRAQHAEEKKHPNTCPGCESHYRDEELAVHL
                     RVCPQCGHHFSVRAFERIAQLADPGSFEEADADIRSADPLAFFDLKPYAERLVEAELA
                     TGLGDAMVTGGATVEQRPCGLAVMDFSFMGGSMGAVIGEKFVRACERAASDGVPLVSV
                     SASGGARMQEGILALMQLPKTVCAVADLHDSGSALISVLAHPTTGGVLASFASIGDVV
                     LAEPGALMSFAGPRVVAQTTREKLPDDFGRAESNLRLGHVDAIVSRAELRSTVGRLLR
                     LFGEA"
     gene            complement(3077..3544)
                     /locus_tag="AC150V_05325"
     CDS             complement(3077..3544)
                     /locus_tag="AC150V_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3762378.1"
                     /translation="MRGLAARGLRLAVVWLDAHGDLNTPASSPSGDAWGMPLRMLLDE
                     GTVRAADVALVGARNLDPPEAEFLAATGIDDGAARAITGVDAVYVALDTDVLDPAVIA
                     SFMPEPAGLTEESCVSLLRQVAAETPLAGAGVTGLRLEADPAVLTRLLRALGL"
BASE COUNT          578 a         1321 c         1174 g          539 t
ORIGIN      
        1 tcgcgcacac cgagtacgag cgacccgaag ccggagctcc gccggccgcg acccgcgtgt
       61 agccggcgat gacgaactcc tgccgccggc gcactttgag cttgcgccag tcgttcgtcc
      121 tccggccggg aatgtaacgg gaggatgcgc gcttggcgac gatcccctcg aaaccctcgc
      181 ccgccgcggc gtccagaagc gcgtgcccgt cctcgaacga cggcgagacg aacacggtcg
      241 gacgtccggg caccacagcg gcctcgaggc gcgctcgccg ctcggcgagc ggcaggtcga
      301 cgagcgtctc gtcatcgagc tcgagcgcgt cgaagagagc gagtaccagg gttcccgcgc
      361 cctgctgcag aagcccgaat tgcgaacgac ccgcttcgtc gagagcgcag atctcgccgt
      421 ccagcacggc ggaagacgcg cgaacggctc gcggaatctc ccgtgcgacg gtcgggaagc
      481 gctcggtgag atcgttcccg ttccgactcg tcagcgcgac cttcccgccg tcgacggcga
      541 taatggcgcg aaaaccgtcc cacttcggct cgaacaccca gccctcaccg ctcgggacga
      601 cctccgccga cgatgccagc atcggctcgt aggtcgcccg ctgctgcgcg acaccgtcct
      661 tgcagatcaa cagccagttc cgcggatcgc cgtcgagcga cgcggcgacg agcgtccaca
      721 ctcccgcaag acgccggccc tccagacgaa cggtgaaccc cccgtcgcgc ttctgctcga
      781 cgagggcata ggtgccccgg tcccagatct cgaccgttcc tgcgccgtac tggccagccg
      841 ggatcgttcc ctcgaagtcg gcgtagtcga gcggatgatc ctcggtctgg acggccagac
      901 gtcgctctcc cttgcgggtc gggattccct tcggaaccgc ccagctcgcg agcaccccgt
      961 ctcgttcgag ccggaagtcg tagtgcagac ggcgggcgtc gtggcgctgg acgacgaagc
     1021 gggcgtctgc gccggactca ccgcccgaga acggctccgg cgtccgcgcc gagtcacgtt
     1081 tgcggcggta ctcgtcgagc ggcacccaga atcagcccta gcccttcgaa gttccttaac
     1141 ataacacgac ttctgcctgc atatggccgg tttttggcca ggggataagc ctgtggaagc
     1201 tgtggagaac cggtcggtag ttttgtatct tctcttacaa agcaggcgcc tgcgcgagca
     1261 cgcccatcgc cctgtacttc gcccgtcgtc gtcggaggag atcgtccgtc gagaccccgt
     1321 cgaggtcgga gaacgcggcc accaccgacg cccggagaaa ctcggctgcc gcatcgtgat
     1381 ccaggtgcgc gccgcccggc ggctccggca ccacggcgtc gatcactccg agctcgagac
     1441 agtgtgcggc atccggcttg aaggccgaag ccgccttgcg cgcctcggca gcgtcgcgcc
     1501 acaggatggc cgcgcagccc tcgggtgaga tcacggagta gatcgcgttc tcctgcatca
     1561 gcacgcggtc cgcgacggcg atcgcgaccg cgccgcccga gccgccctcg ccgatcacga
     1621 cggagacggt cggcacctcg aggcgcacca ttacggcctg cgaacgggcg attgcgccac
     1681 cctgaccggc ctgttcagca gcgactcccg ggtacgcgcc gggcgtgtcg acgagggtca
     1741 ggagcgggaa cccgtgccgc tgcgccagcg ccatcgtccg catcgccttg cggtagccct
     1801 gcgggtatgc catgccgaag ttacggctcg cccgctcctt gatgtcgcgc cccttctggt
     1861 gcccgaccac cgcgatcgtg actccgtcga gcttgccgag agccgcgacg atcgcctcgt
     1921 cgtccatgcc gccccggtca ccgtgcagct cgaagcagtc gtcgagtatc cgctccacgt
     1981 agtcgagcgt gtacggccga tcctgatgcc tggccagctc gacggaacgc catacctcct
     2041 cgtccgtcgc gtcggcttga aggtgctcta gctggcgctg gagccgttcg agctcgccgg
     2101 acaccttcgt gccccggagg agcttgacct tgccgagcct gctcagacgg tctcgcagcc
     2161 gtagctccgc ctcgcgcgtc atgcctctcc gaagagccgc aacagccgcc ccaccgtcga
     2221 ccggagctcc gcgcgcgaca cgatggcgtc gacgtgccca aggcggaggt tggactcagc
     2281 ccgtccgaag tcgtccggaa gcttctcccg cgtcgtctga gcgacgaccc ggggtccggc
     2341 gaaggacatc agcgcaccgg gctcggcgag gacgacgtct ccgatgctcg cgaagctggc
     2401 cagcacgccg ccggtcgtcg gatgcgccag gacggagatc aaggcgctgc ccgaatcgtg
     2461 gaggtcggcc actgcgcaga cggttttcgg cagctgcatg agcgcgagga ttccctcctg
     2521 catccgagcg ccacccgagg ccgacacgga cacgagcggc acgccgtcgg aggcggctcg
     2581 ctcacacgct cgcacgaact tctcgccgat cactgcgccc atcgacccgc ccatgaacga
     2641 gaagtccatc accgcgagac cgcacggtct ctgctcgacg gtcgcgccgc cggtgaccat
     2701 cgcgtcgccg agcccagtcg cgagctcagc ttcgacgagt cgctccgcat acggcttcag
     2761 atcgaagaat gcgagcgggt ctgccgagcg aatgtcggcg tccgcctcct cgaagctccc
     2821 cgggtcggcc agctgggcga tgcgctcgaa agcccgcacg gaaaaatggt gaccacactg
     2881 cgggcacacg cgcagatgca ccgcgagctc ctcgtcacgg tagtgcgatt cgcagcccgg
     2941 gcaagtattg gggtgcttct tctcctcggc gtgttgcgcg cgcttgtgct caatcgagac
     3001 gtcgatccgg tccgccgcgg ccatgcgcgc catcttagtg ctcgcggcct agcggctcat
     3061 tccgccacct ggtccgtcac aggccgagcg cgcggagcaa ccgggtcagc acggccggat
     3121 cggcctccaa gcgcagaccg gtcactccgg cgcccgcgag cggcgtctcg gcggcgacct
     3181 gccgcagcag ggagacgcag gactcttccg tgagcccggc gggctccggc atgaagctcg
     3241 cgatcaccgc gggatcgagc acgtcggtgt cgagagcgac gtagaccgcg tccaccccgg
     3301 tgatcgcccg cgccgcgccg tcgtcgattc ccgtcgcagc caagaactcc gcctccggcg
     3361 gatccaggtt ccgtgcgccg acgagcgcga cgtcggccgc tctcaccgtt ccctcgtcca
     3421 gaagcatccg cagcggcatg ccccacgcgt cgccggaggg agacgacgcg ggcgtgttca
     3481 gatcgccgtg tgcgtcgagc cagacgacgg cgagccgcaa cccgcgcgcc gccagtccac
     3541 gcacggcgcc gacgtgggcg caacagcagc cccctcacac gagcggccgc tcggggagcg
     3601 aggcggcgag ag
//
LOCUS       DBNGNK010000184        12784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1249107,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000184 DBNGNK010000000
VERSION     DBNGNK010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 12784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12784
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1249107"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..242
                     /locus_tag="AC150V_05330"
                     /pseudo
     CDS             45..242
                     /locus_tag="AC150V_05330"
                     /inference="COORDINATES: protein motif:HMM:NF022188.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
     gene            351..1115
                     /gene="panC"
                     /locus_tag="AC150V_05335"
     CDS             351..1115
                     /gene="panC"
                     /locus_tag="AC150V_05335"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR3762379.1"
                     /translation="MQRSVAGVRAALALRRVGRIGLVPTMGALHEGHLSLLRAAREEC
                     DTVVMSLFVNPTQFGDNGDLSRYPQDEERDLAVAAEAGVDVVFAPPAEEMYPSGFQTS
                     VEVTELGAILEGEARPGHFRGVATVCLKLFNVVKPDVAYFGQKDAQQVEVVRRMAADL
                     NLELEIRTLPTVRDDDGLALSSRNALLSEEERERALALPRALATRDPDRARALLADLE
                     VDYVDVAPFDPPVLAAAVRVGGTRLIDNVPLEGEPA"
     gene            1112..2005
                     /gene="panB"
                     /locus_tag="AC150V_05340"
     CDS             1112..2005
                     /gene="panB"
                     /locus_tag="AC150V_05340"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3762380.1"
                     /translation="MSTRPRSPAPNTPAPGKLAVTELAELKARRQPIAMVTAYDAPSG
                     RLADAAGADMILVGDSAAMTVLGHDSTVPATMEEMLVLTRAVTRGARRPLVVGDLPFG
                     SFQVSDEDAVRNAIRFVKEAGADAVKLEGAGRMLSRAAAIVEAGVPVMGHIGLTPQSA
                     TALGGFKAQGRTAEKAQRLYDDALSLQAVGCFSLVLEAVPAPVAKRITQRLAIPTIGI
                     GAGAHCDGQVLVWHDLLGLYEGRAPRFVKRYASIGEEIRVALEAYVEDVREHRFPEDV
                     HTYSMPEEELALFEADVGSRM"
     gene            complement(2052..2675)
                     /locus_tag="AC150V_05345"
     CDS             complement(2052..2675)
                     /locus_tag="AC150V_05345"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR3762381.1"
                     /translation="MSAQREWWRRAPRVLVQPREVFASVAETDEEDLAARQEPVLLII
                     GLAGIGGVLMSPTWGTVMNSYEVDWLVFAVVTFVAGAMYGAAGYFLIGGALWLGARGM
                     GSWGTWRLARHVVAFSAAPLALSLLVTLPLQIALWGERSFESGGPDEGALGAFVVALE
                     LCFVAWAILLLALGVKVVYGWTWARAAGALALLSILLAAFIALPSVL"
     gene            complement(2725..5334)
                     /locus_tag="AC150V_05350"
     CDS             complement(2725..5334)
                     /locus_tag="AC150V_05350"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3762382.1"
                     /translation="MAAIETEITKQAAPPATGANLSRPRRFRLPTLAPSPWPVAAIAA
                     ALAAAVFFAGIRGIGEPLDPDIAPLWVFAVIFLGAELYVIHARSRTELVALSPHDAGL
                     VLGLFLLTPELLLLSQVAGGLVALIAARRDRPGTVAARIAALGLSTCAALLVFHTLVG
                     DHDHSAIVGWGAAAVAVSVAAVLVLGVTFLARGREAFGERVSRTIVIALAGALASASI
                     ALAGVAMARTGETAAVLLIVPFACFALALRAYTSERLRLEHLRRLYESMRAVQMEPVP
                     DSGVSELLTAARRVVGADVSRLVINASDGSQPLVAFATTAAEASLEPMNLSTAERNVV
                     DATLGSGTGVMLSRRVGGHGAKLLAELGLGEALMTPLHSDDGIRGALLVGNRPEAERR
                     LGKEDLRALETFAGHAGVVLENDRLERSLNELTQLKEQLRHQAFHDALTGLPNRTLFA
                     ERVGHALEGDRSVPVTVLFLDLDDFKTINDSLGHLVGDELLSAVAGRLKNAVRPGDIP
                     ARLGGDEFAVLARGIGPLEAEVVADRLVHSLDDSFLIEGNDIAVHASVGIASGLPGSV
                     TSDELLRNADVAMYDAKRAGKRQFVRYEPRMHRRVRQRQELAAALERAVARGEIGVHY
                     QPIVDIKTKRVVSLESLARWDRRENGLVLPTSFIPLADELGFMIEIGRAVLRESCRQA
                     RAWQLAFPGHEKLTVNVNLAPSELHDPMLAEDVHEILAETKLAPERLVLEITESGVML
                     CPETALRTMEQLRELGVLLALDDFGTGHSSLAHLREFPIDTLKIASPFVAGLPDGDLD
                     AVFIDAIVRLATSLGLDVVAEGIETPGQSDAVAGLGCTHGQGFYYGEPLGRLGVSSYL
                     GAEELPTATISRA"
     gene            complement(5465..8305)
                     /gene="uvrA"
                     /locus_tag="AC150V_05355"
     CDS             complement(5465..8305)
                     /gene="uvrA"
                     /locus_tag="AC150V_05355"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR3762383.1"
                     /translation="MASEKLVVHGAREHNLKDVTVELPRHALICVTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLQMMEKPDVDSIDGLSPAISIDQKTTSRNPRSTVGT
                     VTEVYDYLRLLYARVGRPHCPVCGRPIAGQSLDQIVEQILALPEGTRFTVNAPVVRDR
                     KGEFKDVLDELRRDGFTRAKVDGEQRLLEEPIELDKKLKHSIDVVVDRLVMKPDLRQR
                     LTQSVETAGGLAEGLVAVDLVDDGDEILFSENFACPDHGISLPELEPRVFSFNSPHGA
                     CPRCTGLGAQLEIDPDLLVPDASLSLAEGALVPWSVGNSGFYEGVVQAIAERYEIDTD
                     VSWRDLAPEQQDLFLNGTGGEKIFVTYRNRMGRKRKYAMAFEGISKSLERRYRETDSA
                     QQRERIEEYMSFRPCPACDGARLKPEVLAVTVGQLSIHEFTRMSVAGALEFLDGLELT
                     KTEELIGHRIVKEIRERLTFLADVGVGYLQLDRASKTLSGGEAQRLRLATQIGSQLVG
                     VLYILDEPSIGLHQRDNNRLIGTLELLRDLGNTVLVVEHDEQMMRSADWLVDMGPGAG
                     EHGGYVVAEGPATSVMRADASITGQYLAGARRIAVPPRREDDRGSFVVRGAAQHNLKG
                     IDVEFPLAKLVCVTGVSGSGKSTLVNEIVYKALANRLNKMRVKPGDHLDVEGIEAFDK
                     VIEIDQTPIGRTPRSNPATYTDLFTHVRELYSLTPEAKVRGYKPGRFSFNVRGGRCEA
                     CKGDGTIKIEMHFLPDVYVPCETCHGRRYNKETLEVRFKGKSIADVLQMSVEEALAFF
                     AKIPKIRRRLQTLHDVGLDYVKLGQPATTLSGGEAQRVKLASELSKVATGRTLYILDE
                     PTTGLHFADIEKLLEVLQRLVDSGNTVIVIEHNLDVIKQADWIVDLGPEGGEEGGELI
                     ATGTPEQVAETAGSYTGEFLQRVLAAPAAAAA"
     gene            8414..8770
                     /locus_tag="AC150V_05360"
     CDS             8414..8770
                     /locus_tag="AC150V_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762384.1"
                     /translation="MADAGLFIGWGEVVRGREIEAVETFNSTMAYWMRLQEEGVIEGV
                     EPVFLEPHGGELQGFFIVRGEAEKLAALRVDQEFQEVIMRASLIADNVGVVGASMGAR
                     LERLMEAYSEQIAAYA"
     gene            complement(8843..9277)
                     /locus_tag="AC150V_05365"
     CDS             complement(8843..9277)
                     /locus_tag="AC150V_05365"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3762385.1"
                     /translation="MASTKQVAEATVEAFNSHDAGRIRAMYADDVVFEAPGDVRYEIA
                     DAATEYAMSWLRAFSDSRVTVHNAVVAGEWAVHQFTFAGTHDQPLVGPDGEIPATHRR
                     LEGRGVDVLRIVGDKVVEEYLYFDQVQVMTQLGLMAEAAATA"
     gene            complement(9377..9613)
                     /locus_tag="AC150V_05370"
     CDS             complement(9377..9613)
                     /locus_tag="AC150V_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762386.1"
                     /translation="MSEAPEIFDDLYLGLRAGGAIRKQRRGEELTTEEQEALGRWQRL
                     SLWRKVVAIGAFAVGTFGLGFTLGGLIFGRWRRA"
     gene            9641..10453
                     /locus_tag="AC150V_05375"
     CDS             9641..10453
                     /locus_tag="AC150V_05375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3762387.1"
                     /translation="MPEYRAIHVSDLHRGKRESARVDSALGRLAEQIAPNLVIASGDL
                     ANRGTREQLERSRELLGELGSELLVVPGNHDIPYSVPGRFTHPWREFERVFGSTEPVV
                     ENDALVAVGLNSVRPWRQQGGRLRAAQLEHAAAAMRRAPPSALRVVVLHHHLAWAPWR
                     GMHKFPVTHRDDVLARLGAAGAELVCSGHIHQSTAVERREIQALDEPTSGALVLATAP
                     GYGRPRPRRTGECHGLHVYEWDAESIAIVTHIWDGRDFAPTARRTFTRATRL"
     gene            10457..11941
                     /locus_tag="AC150V_05380"
     CDS             10457..11941
                     /locus_tag="AC150V_05380"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3762388.1"
                     /translation="MEAYEKLERRLREVTDLQTSAELLFWDQTVKMPPTGAAVRAEQL
                     ATLQRLAHELFIDDEVGELLEKLRPYEESLEYDSDEASMIRTARRDWEKDRRVPGELA
                     AEMMRVASEAMEAWARARHDSDYGLFRPWLERTIELKLRYIDCFEPAEPYDAALDDFE
                     PGMTTPEVRAVFERLKEVLVPLIADASKDREELPPGPYPEQAQEQLSLELVRAWGFDD
                     RSWRLDRTVHPFCANSGSHDIRLTTRYSETDLTSLFTAMHECGHGLYEFGVADSLERT
                     PLGSGCSSALHESQSRLWENIVGRSLPFWRWFYPRVQKAFPKTLRGVGVERFPAAVNR
                     VTPSFIRVDADQATYGMHIILRFELEPELLAGTLDPKDLPDAWNERFEQYLGLEVPDD
                     RRGVLQDVHWSGGSFGYFPTYQLGTVISVQIWEAAAAALPDVEAQIERGEFDELGAWL
                     REHLYRHGRKFTPQETLERVVGGPIDPEPYIRYLESRFGKAAAA"
     gene            complement(11938..>12784)
                     /locus_tag="AC150V_05385"
     CDS             complement(11938..>12784)
                     /locus_tag="AC150V_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3762389.1"
                     /translation="TFRFTHRVGDLLVAVGIVWLAAALAAALLLDYWNLGWWLGHGFE
                     VVGIAVVGVPVAIDLRRGARSSRPLVGDLRGVDLVVSEEAFLGSHIRALMVSLAAKDA
                     YTEGHVRRVALRAVEVGELLGLAPNRLRELAAGGLLHDIGKLTVADEILKKPGPLTES
                     EFEVIREHPVLGRELLNELGGFDRVVLELVLSHHERLDGSGYPNGISADDLSLEVRIL
                     GACDVYDALCSARVYRPAWSHERAIGLLRDGAGTEFDPRVVDALERVLGREQERSDQD
                     VVIAV"
BASE COUNT         2068 a         4389 c         4397 g         1930 t
ORIGIN      
        1 tggcggagac gctcggcctc gagcccttcg cgctcgacga cgggctgcgc gccgcctacc
       61 acgccggggc cgtgatcgcc tcgaactacc tcgtgacgct acgccgtgcc gggggcagcc
      121 tgctggaggc cgcgggagcc ccgccggaag gtctagaccc gctcatgcgc cgcacgatcg
      181 acaacgactt cgcgctcacc gggccgatcg accggggcga ctggggcacc gtcgagcggc
      241 accttggcgc gatcgagtcg gagcggcggg agctccttgc cgcctaccgt gctcttgccg
      301 agctgacggc gcaccagatc ggcacggctc ccgcctcgcg gacgggcttc ctgcagcgca
      361 gcgtcgcggg cgtccgcgcc gcgctggccc tgcggcgcgt cggccgcatc ggtctcgtcc
      421 cgacgatggg cgcccttcac gagggccatc tctcgctcct ccgcgccgcc cgcgaggagt
      481 gcgacacggt cgtgatgagc ttgttcgtca accccacgca gttcggcgac aacggcgacc
      541 tgtcgcgcta tccgcaggac gaggagcgtg acctcgccgt cgcggcggag gccggcgtgg
      601 acgtcgtctt cgcgcccccg gctgaggaga tgtatccgag cggattccag acctcggtcg
      661 aggtcacaga gctcggcgcg attctcgagg gcgaggcgcg gccgggccac ttccggggtg
      721 tcgcaaccgt gtgcctgaag ctgttcaacg tcgtcaagcc ggacgtcgcc tacttcggac
      781 agaaggacgc acagcaggtc gaagtcgtcc ggcggatggc cgctgatctg aacctcgagc
      841 tcgagatcag gaccttgccg accgtccgcg acgacgacgg tctggcgctc tcgtcgcgca
      901 acgcactcct ttcggaggaa gagcgggagc gggcgctggc tctcccacga gcgctcgcca
      961 cgcgcgatcc cgaccgcgct cgggcgctgc tcgcagacct ggaggtcgac tacgtcgacg
     1021 tcgcgccgtt cgaccctcct gtcctggcgg ccgccgtgcg cgtcggcggc acccggctga
     1081 tcgacaacgt ccccctggaa ggagaacccg catgagcacc cgaccgcgca gccccgcgcc
     1141 caacacaccc gcacccggca agctggccgt tacagagctc gccgagctca aggcgcggcg
     1201 ccagccgatt gcgatggtga ccgcctacga tgcaccgtct gggcgactcg cggacgcggc
     1261 cggcgccgac atgattctgg tgggcgactc ggccgcgatg acggtgctgg ggcacgactc
     1321 gacggtgccg gcgacgatgg aggagatgct cgtcctcaca cgggccgtca cccgtggcgc
     1381 ccggcgcccg ctggtggtcg gcgacctgcc gttcggctcc ttccaggtct cggacgagga
     1441 cgccgtccga aacgcgatcc gtttcgtcaa ggaagcgggt gccgatgcgg tgaagctcga
     1501 aggtgctgga cgaatgctct cccgggcggc cgcgatcgtc gaggcgggag tccccgtcat
     1561 ggggcacatc gggctcacgc cgcagtcggc gacggcgctc ggcggcttca aggctcaggg
     1621 tcgaacggcg gaaaaggcgc agcgcctcta tgacgacgcg ctctctctgc aggcagtcgg
     1681 ctgtttctcg ctcgtgctcg aagccgttcc ggcgcccgta gcgaaacgga tcacacagcg
     1741 tctcgcgatc ccgacgatcg gtatcggtgc cggcgcgcac tgcgacgggc aagtgctcgt
     1801 ctggcacgat ctgctgggcc tctacgaggg tcgggcgccg cgcttcgtga agcgctacgc
     1861 gtcgatcggc gaagagattc gagtggcgct ggaggcgtac gtggaagacg tccgcgagca
     1921 ccgcttcccg gaggacgtcc acacgtattc gatgcccgag gaggagctgg cgctcttcga
     1981 ggccgacgtc ggctcccgca tgtagagccc gattcacgcc caagacgacg accggggcgc
     2041 taccgtcacg gctagagcac cgacgggagc gcgatgaaag cggcgagcaa gatcgagagc
     2101 agagcgagcg cgccggcggc gcgtgcccac gtccagccgt agacgacctt cactccgagc
     2161 gcgagcagca ggatcgccca cgctacaaaa cagagctcga gcgcgacgac gaacgcgccg
     2221 agcgcgcctt cgtccgggcc accgctctcg aacgagcgct cgccccagag ggcgatctgc
     2281 agcggcagcg tgaccaggag cgaaagcgca agcggtgccg cgctgaaggc gacaacgtgg
     2341 cgggcgagac gccacgtgcc ccagcttccc atgccgcgcg cgccgagcca cagcgccccg
     2401 ccgatgagga aatagcccgc ggcgccgtac atcgcaccgg cgacgaacgt cacgaccgcg
     2461 aagacgagcc agtcgacctc gtagctgttc atcacggtgc cccacgtcgg gctcatcagc
     2521 acgccgccga tcccggccag cccgatgatc aggagaacgg gctcctgccg ggcggccaga
     2581 tcttcctcgt ccgtctctgc caccgacgcg aacacctcgc gcggctggac gagcacgcgc
     2641 ggcgctcgcc gccaccactc gcgctgcgcg ctcatggctc gagcgtagcc gcggacgctt
     2701 ccgcccgtcg gcggcgaacg cccgctacgc cctactgatc gtcgcggtcg ggagctcctc
     2761 ggcgccgaga tacgaggaca caccgaggcg gccgagcggc tcaccgtagt agaagccctg
     2821 gccgtgcgtg caaccgagtc ccgccacggc gtcggactgg ccgggcgtct cgattccctc
     2881 ggcgacgaca tcgagcccga gcgacgtagc gagccgcacg atcgcgtcga tgaagacggc
     2941 gtcgaggtcg ccgtcgggaa ggcccgctac gaacggactg gcgatcttga gggtatcgat
     3001 ggggaactcc cgcaagtgcg cgagcgagga atggccggtg ccgaagtcgt cgagagccag
     3061 caggacgccg agctctcgca actgctccat cgttcggagc gccgtctcag ggcacagcat
     3121 gacgccgctc tcggtgatct cgaggacgag tcgctccgga gccagcttcg tctcggcgag
     3181 gatctcgtgc acatcctcgg cgagcattgg gtcgtgtagc tcgctcggcg cgaggttgac
     3241 gttgaccgtc agtttctcgt ggccgggaaa ggcaagctgc cacgcgcgtg cctgtcggca
     3301 cgactcgcgg aggacagctc ggccgatctc gatcatgaag ccgagctcgt ccgcgagcgg
     3361 gatgaaactc gtcggcagca cgagtccgtt ctcgcgccgg tcccagcggg cgagcgactc
     3421 gagggagacg acccgcttcg tcttgatgtc gacgatgggc tggtagtgga cgccgatctc
     3481 gcctcgggcc accgcgcgct cgagcgccgc agcgagctcc tggcgctggc ggacgcggcg
     3541 gtgcatcctc ggctcgtaac gtacgaactg acgcttgcct gcccgcttgg cgtcgtacat
     3601 cgcgacgtcg gcgttccgaa gcagctcgtc actcgtcact gagcccggta gaccactggc
     3661 aatgccgacg ctcgcgtgca ctgcgatgtc gtttccctcg atcaggaagg agtcgtcgag
     3721 cgagtgcacg aggcggtcgg cgacgacctc ggcctcgagg ggcccgatac cgcgcgcgag
     3781 cacggcgaac tcgtcaccgc cgaggcgtgc cggaatgtct cccggccgca ccgcgttctt
     3841 cagtcgcccc gctacggccg acaagagctc gtcgccgacc aggtggccga ggctgtcgtt
     3901 gatcgtcttg aagtcgtcga gatcgagaaa gagcaccgtc acaggcacgc tccggtcgcc
     3961 ttcgagagcg tgcccgaccc gctcggcgaa aagcgttctg ttcggaagac cggtcaacgc
     4021 gtcatggaag gcctgatgcc gcagctgctc cttgagctgc gtgagctcgt tcagcgaccg
     4081 ctccagccgg tcgttttcga gcacgacacc ggcgtgaccc gcgaacgtct cgagcgcgcg
     4141 caggtcttcc ttgccgagcc gacgctcggc ctctggacgg ttgccgacga ggagggctcc
     4201 acggattccg tcgtcgctgt ggagcggcgt catgagcgcc tcgccgagac ccagctcggc
     4261 cagcagcttc gcgccgtgac cgcctacccg acgcgagagc atcactccgg tgccgctgcc
     4321 cagggtggcg tcgacgacgt tgcgctcggc ggtggagaga ttcatcggct cgaggctcgc
     4381 ttcggcagcg gtcgtggcga aggcgacgag cggttgcgac ccgtcgctcg cgttgatcac
     4441 cagccgcgag acgtcggcgc cgaccacgcg gcgagccgca gtcagcagct cactcacgcc
     4501 ggaatcgggt acgggctcca tttgcaccgc acgcatcgac tcgtataggc ggcgcaggtg
     4561 ctcgagacga agccgctcgg atgtgtacgc acgaagtgcg agggcgaaac aggcgaacgg
     4621 cacgatcaac agcaccgcgg ccgtctcccc ggtgcgtgcc atcgcaaccc ccgccagcgc
     4681 gatgctcgcg cttgcgagcg cgccggcgag cgcgatcacg atcgttcgcg aaactcgctc
     4741 tccgaacgcc tcacgaccgc gggcgaggaa cgtgaccccg agaaccaaga ctgccgcgac
     4801 cgagacggcg acggctgccg ccccccagcc gacgatcgcc gagtggtcgt ggtcgccgac
     4861 gagcgtgtgg aagacgagga gtgccgcgca ggtgctgaga cccagcgccg cgatgcgcgc
     4921 agcaaccgtg ccgggccggt cgcggcgcgc ggcaatgagc gcaacgagac cgccggcgac
     4981 ttgcgacagc agcagcagct cgggcgtcag gaggaagagc ccgagaacca gaccggcgtc
     5041 gtgcggcgag agggccacga gctctgtccg gctgcgagcg tggatgacgt acagctcggc
     5101 accgaggaag atgacggcga atacccagag cggggcgatg tcgggatcca gcggctcgcc
     5161 gatgcccctg atcccggcga agaacacagc cgccgcgagt gcggcggcga tggcagcaac
     5221 gggccaggga gacggcgcca gggtcggcag ccggaagcgg cgcggtcgcg agagattcgc
     5281 cccggtcgct ggaggcgcgg cctgcttcgt gatttcggtc tcgatcgctg ccaacgaaag
     5341 tcagcttctc cccgcgcgca aagcgcatac cgcagtgcac gaaccgtgct cgtgcggctg
     5401 cccatcctct tcccccgagc ggggtaaccc cttctccctc gttcgagtga cggtggtcgg
     5461 acgactaggc ggcggctgct gcgggagcgg ctagcacgcg ctgcaggaac tcaccggtgt
     5521 acgagccggc cgtctcggcc acctgctcgg gtgtcccggt ggcgatcagc tcgccgccct
     5581 cctctccgcc ttcggggccg aggtcgacga tccaatccgc ctgcttgatc acgtcgagat
     5641 tatgctcgat cacgatcacc gtgttcccgc tgtcgacgag tctctgcagc acctccagca
     5701 gcttctcgat atcggcgaaa tggagcccgg tcgtcggctc gtcgaggatg tacagcgtgc
     5761 gtccggtcgc caccttcgag agctccgagg cgagcttgac gcgctgcgct tctccccccg
     5821 agagcgtcgt cgccggctgt ccgagcttga cgtagtccag cccgacgtcg tgaagcgtct
     5881 gcagccggcg ccggatcttc gggatcttgg caaagaaggc gagcgcctcc tcgacggaca
     5941 tttgaaggac gtcggcgatc gacttgccct tgaatcgcac ctccaacgtc tccttgttgt
     6001 accggcgccc gtgacacgtc tcgcagggga cgtagacgtc ggggaggaag tgcatctcga
     6061 tcttgatcgt cccgtctccc ttgcacgcct cacagcgtcc gccccgcacg ttgaacgaga
     6121 agcgccctgg cttgtagcca cgcactttcg cctcgggcgt gagcgaatag agctcacgca
     6181 cgtgggtgaa gaggtccgtg tacgtcgccg ggttcgagcg cggcgtgcgg ccgatcggcg
     6241 tctggtcgat ctcgatgacc ttgtcgaacg cctcgatgcc ctcgacgtcg aggtggtcgc
     6301 cgggcttgac ccgcatcttg ttgaggcggt tcgcgagcgc cttgtagacg atctcgttca
     6361 ccagcgtgga cttgccggag cccgagacac cggtgacgca cacgagcttc gcgagcggga
     6421 actcgacgtc aattcccttc aggttgtgct gcgcggcgcc gcggacgacg aatgagccgc
     6481 gatcgtcctc gcgacgcggg ggcactgcga tccttcgagc gcccgccaga tactgaccgg
     6541 tgatcgaggc gtccgcccgc atcaccgacg tcgccggccc ctctgccacg acgtagccgc
     6601 cgtgctcgcc cgcgccggga cccatgtcga cgagccagtc cgccgagcgc atcatctgct
     6661 cgtcgtgctc gacgaccaga acggtgttgc cgaggtcgcg gagcagctcc agcgtgccga
     6721 tcagccggtt gttgtcgcgc tggtgcagac cgatcgaggg ctcgtcgagg atgtagagga
     6781 cgccgacgag ctgggagccg atctgcgtcg cgaggcgcag gcgctgcgcc tccccgcccg
     6841 agagcgtctt cgacgcgcgg tcgagctgca ggtagccgac gccgacgtcg gcgaggaacg
     6901 tgagccgctc ccggatctcc ttcacgatcc ggtggccgat cagctcctcg gtcttcgtca
     6961 gctcgagccc gtcgagaaat tcgagcgcgc cggcgaccga catgcgcgtg aactcgtgga
     7021 tcgagagctg gccgacggtg accgccagca cctccggctt gagccgggcg ccgtcgcagg
     7081 ccggacacgg gcggaagctc atgtactcct cgatgcgctc ccgctgctgg gcggagtccg
     7141 tctcccggta acggcgctcg agcgacttcg agatcccctc gaaggccatg gcgtacttgc
     7201 gcttgcgtcc catccggttg cggtaggtga cgaagatctt ctcgccgccc gttccgttca
     7261 gaaacaaatc ctgctgctcc ggcgcgaggt cgcgccagga cacgtcggta tcgatctcgt
     7321 agcgctcggc gatcgcctgg acgacgccct cgtagaagcc ggagttcccg accgaccacg
     7381 gaaccagggc tccctcggcc agcgagagcg aagcgtccgg gacgagcagg tcgggatcta
     7441 tctcgagctg cgcgccgagg ccggtgcagc gggggcaggc gccgtgcgga gagttgaagg
     7501 agaagacgcg cggctcgagc tccgggagcg agatcccatg gtccggacac gcgaagttct
     7561 cggagaagag gatctcgtct ccgtcgtcga cgaggtcgac ggccacgagg ccctcggcca
     7621 ggccgccggc ggtctcgacc gactgggtga gccgctggcg caggtcgggc ttcatcacga
     7681 gccggtcgac gacgacgtcg atcgagtgct tcagcttctt gtcgagctcg atgggctcct
     7741 cgagcaggcg ctgctcaccg tcgaccttgg cgcgggtgaa gccgtcgcgg cgcagctcgt
     7801 cgagcacgtc cttgaactcc cccttgcggt cgcggacgac gggcgcgttg accgtgaacc
     7861 gcgtgccctc cggcagggca agaatctgct cgacgatctg gtcgaggctc tgcccggcga
     7921 tcggccgacc gcagacggga cagtggggcc ggcccactcg cgcgtagagc agccggaggt
     7981 agtcgtacac ctcggtcacc gtcccgacgg tcgagcgcgg gttgcgcgac gtcgtcttct
     8041 ggtcgatcga gatcgccggc gatagcccgt cgatcgagtc gacgtccggc ttctccatca
     8101 tctggaggaa ctggcgcgcg taggccgaca gcgattcgac gtaacgacgc tgcccctccg
     8161 cgtagatcgt gtcgaaggcg agcgaagact tgcccgagcc cgatagtccg gtcacgcaga
     8221 tcaacgcgtg ccgggggagc tcgacggtga cgtccttcag gttgtgctcg cgggcgccgt
     8281 ggacgaccag cttctcggag gccatagccg atgcagttta ctcaccgaac gtacgttcgc
     8341 caagcgaaat agattgcgcc gatcggcgca aagtgcaatg ctcgtcgcgt ccctctcaaa
     8401 gcaggaggtc ttaatggctg acgcagggct cttcatcggc tggggcgagg tcgtgcgcgg
     8461 gcgcgagatc gaggcggtcg agacgttcaa ctccacgatg gcgtactgga tgcgcctgca
     8521 ggaggaaggc gtgatcgagg gcgtcgagcc tgtcttcctc gagccgcacg gcggagagct
     8581 ccagggcttc ttcatcgtgc gcggcgaggc cgagaagctc gctgcgcttc gcgtcgacca
     8641 agagttccag gaagtgatca tgcgggcgag cctgatcgcc gacaacgtcg gcgtcgtcgg
     8701 tgcgagcatg ggcgcgcggc tcgagcgcct gatggaggcg tacagcgagc agatcgccgc
     8761 gtacgcgtag gcctcacccg ccgacgaggt tccggcggcc gcgtgcccct cgccgcggcc
     8821 gccggtcagc tcgtgcagcc tgctaggcag tcgccgcagc ttcggccatc agaccgagct
     8881 gcgtcatcac ctgcacctgg tcgaagtaca ggtactcctc gacgaccttg tcgcccacga
     8941 tccgaaggac gtcgactccg cggccctcca ggcggcggtg ggtcgccggg atctctccgt
     9001 ccgggccgac gaggggctgg tcgtgcgtcc cggcgaacgt gaactgatgc acggcccatt
     9061 cgcctgcgac gacagcgttg tggacggtga cacgcgagtc ggagaacgcg cggagccacg
     9121 acatcgcata ctccgtggcg gcgtcggcga tctcgtagcg gacgtcaccg ggggcctcga
     9181 acacgacatc gtcggcgtac atcgcgcgga tgcggccggc gtcgtgagag ttgaacgcct
     9241 cgaccgttgc ctcggccact tgcttggtgc ttgccatgag cttccctcct ggctcaggtg
     9301 tgatccgata caaggggttc aggcacacta acgcgcctcg tgtgaagcgc caagccccgg
     9361 acgggtgagc gggcggtcag gcgcggcgcc agcggccgaa gatcaggccg ccgagcgtga
     9421 atccgagtcc gaacgtgccg accgcgaagg cgccgatagc gacgaccttc cgccagagcg
     9481 agaggcgctg ccaacggccg agcgcctcct gttcctcggt cgtcagctcc tcgccccggc
     9541 gctgcttgcg gatcgccccg cccgcgcgaa gacccaggta gaggtcgtcg aagatctcag
     9601 gggcctcgga catcgcatcg agtgtatagc ctcgccaccg ttgcccgagt accgcgccat
     9661 tcacgtctcc gatctgcacc gcggcaagcg cgagtccgcg cgggtcgact ccgcgctcgg
     9721 ccgcctggcc gagcagatcg cgccgaacct cgtgatcgcg agcggcgatc tcgccaaccg
     9781 gggcacccgc gagcagctcg agcgctcgcg tgagctgctc ggcgagctcg ggtcggagct
     9841 tctcgtcgtc cccggcaacc acgacatccc gtacagcgtc cccgggcgat tcacgcaccc
     9901 atggcgcgag ttcgagcgcg tattcggctc caccgagccc gtggtggaaa acgacgctct
     9961 cgtcgcggtg gggctcaact cggtgcggcc gtggcgccag caggggggcc gtctccgcgc
    10021 cgcccagctg gagcacgcgg cggcggcgat gcgccgagcg ccaccgtccg ccctgcgggt
    10081 cgtcgtcctt catcaccacc tcgcgtgggc tccctggcgg ggcatgcaca agttcccggt
    10141 gacgcacagg gacgacgtcc tcgcccggct gggcgccgcg ggcgccgagc tcgtctgcag
    10201 cgggcacatc caccagtcga cagcggtgga gcgccgcgag atccaggctc tcgacgagcc
    10261 gacctcgggg gcgctcgtcc tcgcgacggc acccggctac ggccgaccgc gaccgaggcg
    10321 gacgggcgaa tgtcacgggc tgcacgtgta cgagtgggat gccgagtcga tcgccatcgt
    10381 gacccacatc tgggacggcc gggacttcgc tccgacggcg cgccgcacgt tcacacgcgc
    10441 cactaggctc tagcccgtgg aggcctatga gaagctcgag cgcaggctcc gagaggtcac
    10501 tgacctgcag acgagcgccg agctcctgtt ctgggatcag accgtgaaga tgccgcccac
    10561 cggcgcggca gtgcgggccg agcagctcgc caccctgcag cgactggccc acgagctctt
    10621 catcgacgac gaggtcggcg agctgctcga gaagctgcgg ccgtacgagg agtctctcga
    10681 gtacgactcg gacgaggcca gcatgatccg aacggcgcgg cgtgactggg agaaggaccg
    10741 ccgagtgccg ggcgagctcg ccgcggagat gatgcgtgtg gcctcagagg cgatggaggc
    10801 ctgggcccgc gcccgccacg actccgacta cgggctcttc cgcccttggc tggaaaggac
    10861 gatcgagctg aagctccgct acatcgactg cttcgagccc gcggagccgt atgacgcggc
    10921 cctcgacgac ttcgagcccg ggatgaccac accggaggtg cgcgccgtct tcgagcggct
    10981 gaaggaggta ctcgtgccgc tgatcgccga cgcgtcgaaa gatcgggagg agctgccgcc
    11041 cgggccgtac cccgagcagg cacaagagca gctctcgctc gagctcgtcc gcgcctgggg
    11101 cttcgacgac cggtcgtggc ggctggatcg caccgtccat cccttttgcg ccaactcggg
    11161 cagccacgac atacgcctga cgacccgcta ctcggagacc gacctgacgt cgctcttcac
    11221 ggccatgcac gagtgcggcc acggactgta cgagttcggc gtcgcggact ccctggagcg
    11281 gacgccgctc ggcagtggtt gctcgtccgc gttgcacgag tcacagagcc ggctctggga
    11341 gaacatcgtg ggtcgctcgc tccccttctg gcgctggttc tacccgcgcg tgcagaaggc
    11401 gtttccgaaa acgctccggg gcgtcggcgt cgagcgcttc cccgccgccg tcaaccgagt
    11461 cacgccgtcg ttcatccgcg tcgacgccga ccaggcgacc tacgggatgc acatcatcct
    11521 ccgtttcgag ctcgagccgg agctgctggc gggcacactc gacccgaagg acctgcctga
    11581 cgcatggaac gagcgcttcg agcaatacct cgggctcgag gtacccgacg accggcgagg
    11641 cgtcctgcag gacgtccact ggtcgggagg cagcttcggg tacttcccca cctaccagct
    11701 cggcactgtg atctcggtac agatctggga ggcggcggcc gccgcgctac ccgacgtgga
    11761 ggcgcagatc gagcgcggtg agttcgacga gctcggcgcc tggttgcgcg agcacctcta
    11821 ccggcacggc aggaagttca cgccgcagga gacgctcgag cgcgtcgtcg gggggccgat
    11881 cgaccccgag ccctacattc gctatctcga gtcgaggttc ggcaaggcgg ccgccgctta
    11941 gaccgcgatg acgacgtcct ggtcggatcg ctcctgctcc cggccgagaa cccgctcgag
    12001 cgcgtcgacg acacgtgggt cgaactccgt acccgcgccg tcgcgcaaga gcccgatcgc
    12061 ccgctcgtgt gaccacgcag gacggtagac gcgggcggag cacagagcgt cgtagacatc
    12121 gcaggcgccg agaatcctga cctcgagact gagatcgtcc gcgctgatcc cgttcgggta
    12181 gccgctaccg tcgagacgct cgtgatgcga cagcacgagc tcgagcacca cccggtcgaa
    12241 gccgcccagc tcgttcagca gctcgcggcc cagcacgggg tgctcgcgga tcacctcgaa
    12301 ctccgactcg gtcagcgggc ctggtttctt gaggatctcg tcggcgaccg tgagcttccc
    12361 gatgtcgtgg aggagtccgc cggcggccag ctcgcgcagc cggttgggag caagccccag
    12421 cagctcaccc acttcgacgg cgcgcaaggc gacgcggcgg acgtgccctt ccgtgtatgc
    12481 gtccttggcg gcgagcgaga ccatgagagc tcggatgtgt gagccgagga acgcctcctc
    12541 ggagacgacg aggtcgacgc cgcgcagatc accgacgagc gggcgcgagg aacgcgcgcc
    12601 gcgccgcagg tcgatcgcca caggaacgcc gacgaccgcg atcccgacca cctcgaagcc
    12661 gtggccgagc caccagccga ggttccagta gtcgaggagg agtgccgcgg cgagggcggc
    12721 ggcgagccag acgatgccga cggcgacgag gaggtcgccg acgcggtggg tgaagcggaa
    12781 ggtg
//
LOCUS       DBNGNK010000185         3055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_821724,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000185 DBNGNK010000000
VERSION     DBNGNK010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_821724"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="AC150V_05390"
     CDS             <1..1374
                     /locus_tag="AC150V_05390"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3762390.1"
                     /translation="RRLEDVRRAVVREIPDEPVPEDELLASLRTLVFENSVLLGHPRF
                     LAYISGAGTVPGAPADLLAAGLNANVGGWFLSPGATEIELALGRFFAQDVFGLPDTSG
                     GYVVSGGSMANFVGLKAARDNKAGWDVRGKGLAGGPQLTVYLSTETHVVSERAADALG
                     IGGAGVRKLPADAGCRFDVPALRETIARDRDAGARPVAVVGTAGTVATGAIDPLDELA
                     DLCAEEGLWFHVDAAYGGPAMLADDLRPRFIGIERADSIAFDPHKWLYTPHSGGFVVL
                     RDMAHFADSFDFSDVGYVQQDRELTQYGLDLGRLGPQFSRGFSALKVWVSLLAHGRAA
                     YARRISHDAALARYLGELVDAREDFELCAPVGLSITCFRYVPPGLPDGAERATYLDRL
                     NERLMTEIQQDGRAFCSNAVLDGRYALRSCVVNFRTEAEDMELLLDVAAELGAALDRE
                     LRPAGAA"
     gene            1371..1712
                     /locus_tag="AC150V_05395"
     CDS             1371..1712
                     /locus_tag="AC150V_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3762391.1"
                     /translation="MKVVGHSQLTAFVTKDGSTIREYLHTPVQSLAEAQLSPGQATQR
                     HHHAKSEEIYLLVAGGGRIELDGEERDVTSGDAILIPPGSPHRFTAGADGARFLCCCV
                     PPYSDADTFFD"
     gene            1814..2893
                     /locus_tag="AC150V_05400"
     CDS             1814..2893
                     /locus_tag="AC150V_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR3762392.1"
                     /translation="MPTISFARGAPSPDCLDPSLIADCTRAAIESDGLTVLSYGSGGG
                     YGPLRELVAARHGVEPGRVFLTAGGLQGFVFYAAVQLARRPGRVLVEAPTYDRPLKIL
                     AGEGAEIVALPMDDEGLEPDALEAELQRGGDVSFLYTIPTFQNPSGRTLGVERRRRLA
                     ELAAEHSLDVLEDDPYGLVRYEGEAPPSLLSLDQGGTMTFTSSFSKTVAPGLRVGYFV
                     VPADMEAAFDDRAVSTYISPSLLPQATIFQLFDRGAFEPNLERVRGLLRARRDAMLAA
                     LQSEFSDGAAWSRPEGGYFLWLDFPSGTDAADVQARAAAEGVAFVKGADFFAGGGGEG
                     SARLAFSYESPERITDGIARLAALV"
BASE COUNT          417 a         1061 c         1078 g          499 t
ORIGIN      
        1 cgcaggctgg aggatgtccg gcgggccgtc gtgcgcgaga tcccggacga gccggtgccg
       61 gaggacgagc tgctcgcctc gctgcgaacg ctcgtcttcg agaactcggt gctgctcggc
      121 cacccgcgct tcctggccta catctccggc gcggggacgg tgcccggcgc gccggcggat
      181 ctgctggccg cagggctcaa cgcgaacgtg ggcggctggt tcctttcgcc gggcgcgacg
      241 gagatcgagc tcgcactcgg ccggttcttc gcccaggacg tcttcgggct acccgacacc
      301 tcgggcggct acgtggtctc gggaggctcg atggccaact tcgtcggact caaggccgca
      361 cgagacaaca aggccggctg ggatgtccga ggcaagggcc tggcgggcgg gccgcagctg
      421 acggtgtacc tctccacgga gacgcacgtc gtctcggagc gcgcagccga tgcgctgggg
      481 attgggggcg cgggcgtccg gaagctccca gccgatgcgg gttgccggtt cgacgttccg
      541 gcgctacggg agacgatcgc gcgcgatcgt gacgccggcg cccggcctgt cgccgtcgtg
      601 ggcacggcgg gaaccgtggc caccggcgcc atcgacccgc tcgacgagct cgccgacctg
      661 tgcgccgagg aggggctgtg gtttcacgtc gacgccgcct acggcggccc cgcgatgctg
      721 gcagacgacc tccggccgcg cttcatcggg atcgagcgcg ccgactcgat tgcgttcgac
      781 ccgcacaagt ggctgtacac accgcactcg ggagggtttg tcgtgttgcg cgacatggcg
      841 cacttcgcag actcgttcga cttcagcgac gtcggctacg tgcagcagga ccgggagcta
      901 acgcagtacg gcctcgatct cggccgtctc gggcctcagt tctcccgcgg cttctctgcg
      961 ctgaaagtgt gggtctcgct gcttgctcac ggccgggccg catacgcacg gcggatctcg
     1021 cacgacgctg cgctcgcacg ctacctcggg gagctcgtgg atgcgcgcga ggacttcgag
     1081 ctctgcgcgc cggtcggcct ttcgatcacc tgcttccgct acgtaccgcc ggggctgccc
     1141 gacggcgccg agcgcgccac gtacctcgac aggctgaacg agcgtctgat gacggagatc
     1201 cagcaggacg ggcgggcgtt ctgctcgaac gcggtcctgg acggccgcta cgcgctgcgc
     1261 tcgtgcgtgg tcaacttccg cacggaggcg gaggacatgg agctcttgct cgacgtcgca
     1321 gcggagctcg gcgcggcgct cgaccgggag ctgcgacccg caggggccgc gtgaaggtcg
     1381 tcgggcactc gcagctgacc gccttcgtca cgaaggacgg ctcgaccatc cgcgagtacc
     1441 tgcacacgcc ggtgcagagc ctcgcggaag cccagctctc cccgggacag gcaactcagc
     1501 gtcaccacca cgcgaagagc gaggagatct acctgctcgt tgcaggcggt ggccgtatcg
     1561 agctcgacgg cgaggagcgg gacgtgacgt cgggcgacgc gattctgatc cctcccggct
     1621 caccccatcg gttcacagcg ggagccgacg gtgcgcgctt cctgtgctgc tgcgtaccgc
     1681 cgtactccga cgccgacacg ttcttcgact aggaccgctt catcgagccg tctcgggcac
     1741 ggctcgatgg ggcccgttgc cgcacagccg gttgccggaa cggcgtgcca gggtgcatcg
     1801 gtaggctgcg gccgtgccga ccatctcgtt cgcccggggt gccccgtcgc ccgactgcct
     1861 cgatccatcg ctgatcgccg attgcacgcg cgcggcaatc gagagtgacg ggctcaccgt
     1921 cctgtcgtat ggctcgggcg gcggctacgg cccgttgcgg gaattggtcg ccgcgcggca
     1981 cggcgtcgag cctggacgcg tcttcctcac ggctggtggg cttcagggct tcgtcttcta
     2041 cgcggcggtg cagctggcgc ggcgtcccgg gagggtgctt gtcgaggcgc cgacctacga
     2101 ccgccccctc aagatcctgg cgggtgaagg tgcggagatc gtcgccctgc cgatggacga
     2161 cgaggggctc gagcccgacg cgctcgaggc ggagctacag cgcggtggtg acgtgtcgtt
     2221 cctctacacg attccgacct tccagaaccc gagcggccgg acgctcggcg tcgagcgacg
     2281 ccgccgactc gccgagctcg ccgccgaaca ctcgctggac gtgctggagg acgatccgta
     2341 cggtctcgtg cggtacgagg gcgaggctcc cccttcgctg ctgagtctcg atcagggcgg
     2401 caccatgacc ttcacctcgt cgttctcgaa gaccgtcgcg ccggggcttc gtgtcgggta
     2461 tttcgtcgtg cctgccgaca tggaggctgc gttcgacgat cgagccgtct ccacgtacat
     2521 ctcgccgtcg ctgctgcctc aggccacgat cttccagctg ttcgaccgcg gcgccttcga
     2581 gccgaacctc gagcgggtgc gcggcctgct gcgcgcacgc cgcgacgcga tgttggcggc
     2641 gctccagtcg gagttctccg acggggccgc gtggagtcgg cccgagggcg gctacttcct
     2701 ctggctcgac ttccccagcg gcacggacgc cgccgacgta caagcccgcg cggccgccga
     2761 gggcgtggcc tttgtgaaag gtgccgactt cttcgccggc ggcggcggcg agggctctgc
     2821 ccgcctggcg ttcagctacg agtcccccga gcggatcacc gacgggatcg ctcgactcgc
     2881 ggcactcgtc tagccgcgtc ggcttcaggc cacgcactcg ctcgtctgac cgcaggaggc
     2941 tagggcttgc gcttcgcgcg tcgccgcttc gctcgctccc gagcgaacgg aacgatgagc
     3001 ccgatcgcga ggccgaggat cagagcgagc aggatcacga acaccagcgg cgtcg
//
LOCUS       DBNGNK010000186         5345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_604522,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000186 DBNGNK010000000
VERSION     DBNGNK010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_604522"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="AC150V_05405"
     CDS             <1..753
                     /locus_tag="AC150V_05405"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3762393.1"
                     /translation="DATDADGIVLRDAMGGAPPPEGWLERPHAFVELHVEQGPLLDRW
                     DVPVGVVSSVAGLARLAVVLRGAPGHAGTTPMDGRADALVAASTFVLDVRDAAAALPG
                     AVATVGQVAVEPGAANVIPARVTALVDARAPDETTLAALVDAITEAGEGAGAEVETLL
                     RTAPVEMDGDVCDVLRRALEELGLEPRYLPSGAGHDAAVVARAGVPSGMLFVRSRNDG
                     ASHSPAEHSESEDVTLGVEALAKALARLATAR"
     gene            complement(740..1147)
                     /locus_tag="AC150V_05410"
     CDS             complement(740..1147)
                     /locus_tag="AC150V_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008730699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HIR3762394.1"
                     /translation="MLEIHAGGFAFRAQLEEELAPQTVAAFRRLLPLESRIIHVRWSG
                     EAGWIPFGELDLGIGPENATCYPHPGEIVLYPGGVSETEILLAYGYVNFASKAGQLAG
                     NHFATILDGAENLPELGRRFLWEGAQEIVITEQ"
     gene            complement(1199..2422)
                     /locus_tag="AC150V_05415"
     CDS             complement(1199..2422)
                     /locus_tag="AC150V_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR3762395.1"
                     /translation="MATRMTTLAAVLDPYIRDWLDLLLRWLHVIAAIAWIGTSFYFVL
                     LDQSLRPPKRKDDADAGVAGELWEVHGGGFYQVQKYQVAPRVLPDHLAWFKWEAYTTW
                     LSGFALMIVLYYLDARTFLIDPAVADLEPWAAIGLSVGLLVAALVVYEVLCRFVKDDR
                     VLGVLIALAAVASAWIATQLFSPRAASLQVGAMLGTIMAANVLFSIIPAHRELVRAKQ
                     AGREPDPAPGIEAKRRSVHNNYLTLPVLLTMLAGHFSFLYAASYDWLVLVALMAVGVL
                     ARVFFNLRHGGRTLWWIPAVGVAGLIALAFVLQPGNSTSDAKGQNAVSLAEVEPIIAQ
                     RCATCHALAPTQDGFSSPPAGIVLETSDQIVAQADEVGAVVRAGTMPLGNITRMTDEE
                     RDLVVAWADQGGPSE"
     gene            complement(2407..2847)
                     /locus_tag="AC150V_05420"
     CDS             complement(2407..2847)
                     /locus_tag="AC150V_05420"
                     /inference="COORDINATES: protein motif:HMM:NF020907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HIR3762396.1"
                     /translation="MRELPRQLSADELAELFEGRTRFVERLAGEDDPLRRARTLVHEL
                     SEDEKKEILDAHPAIGQPTALSARSAVEQGADAEPAVLEELDRLNRRYERTHGFRFVV
                     FVNRRPKTEILAVLRRRIERPTEEELETALGELVAIAEDRWRRG"
     gene            complement(2823..3125)
                     /locus_tag="AC150V_05425"
     CDS             complement(2823..3125)
                     /locus_tag="AC150V_05425"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033971 - hydroxyisourate hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA];
                     GO_process: GO:0051289 - protein homotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HIR3762397.1"
                     /translation="MHGVSGSLSTHVLDTAAGRPVAGTRVELFQAEDLVAWAETDADG
                     RIAELAGGLEPGVYQLVFRPPSPFFRRISLEVELADDHYHVPLLVSPYGCASYRGS"
     gene            complement(3217..4914)
                     /gene="ade"
                     /locus_tag="AC150V_05430"
     CDS             complement(3217..4914)
                     /gene="ade"
                     /locus_tag="AC150V_05430"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR3762398.1"
                     /translation="MDRLRRRLAVARGDEPADLVVRGGRVLSVFTREWLDVDVAVVDG
                     VIAGLGGYDAENVLDATDRYVVPGLVDAHMHLETSKLLVDEFARLVLPLGTTTVVADP
                     HEIANVLGTDGIHWLLDASSGLPLDVYFTASSCVPASRFESPRRPLEPGDLESLLRRR
                     RMVGLAEMMNFPGVIAGSDHELAKLTIAGVDHVDGHAPGVLGLELQAYAAAGIGSDHE
                     ASTVEEGRERLRAGMWLLIREASAARNLAALVPLVREYGTDRIAFCTDDREPEHIADD
                     GHMNAIVREAVRLGLPPEDAIVLASLNPARWHGLQRRGGLAPGYRADILLLPDLEGFV
                     PEVVLKSGAPVGDFSHPPVPSWLRQTVRLAPIDDDTFRLPWRGGDGLARVIGIVPGQI
                     TTCLLTERPTLADGEVMADAAHDIAKVAVLERHLGTGRIGVGLARGLGLRSGALASSF
                     SHDAHNVVVAGMDDTSMRAAVERIAALGGGLVVADGDGIRAELPLPVAGLLSDLPHSD
                     VIERSRACVAAARALGCTLDAPFQSLAFLALSVIPHLKLTDHGLVDVDRFEIVPLEA"
     gene            4945..>5345
                     /locus_tag="AC150V_05435"
     CDS             4945..>5345
                     /locus_tag="AC150V_05435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3762399.1"
                     /translation="MEVLSPASLDEALRLRSAHPDALVIQGGTDVMVELNFGRARPDV
                     ILNLNGVLELRGWRKENGTLVLGAGLSYAAAETDLAGALPALAEASRTVGSPQIRSRG
                     TIGGNLGTASPAGDALPPLLIEDAEVELASLR"
BASE COUNT          849 a         1865 c         1859 g          772 t
ORIGIN      
        1 gacgccaccg atgccgacgg catcgttctt cgcgacgcga tggggggagc gccgccgccc
       61 gagggctggc tcgagcgccc gcacgcgttc gtcgagctcc acgtcgagca gggtccgctg
      121 ctcgaccgct gggatgtccc ggttggggtc gtgagctcgg tcgccgggct cgcccggctg
      181 gccgtcgtcc tgcgcggcgc gccggggcac gccggaacga cgcccatgga cggacgggcg
      241 gatgcgctcg tcgcggcgag caccttcgta ctcgacgtgc gcgacgccgc cgccgccctt
      301 ccgggcgcgg tcgcgacagt ggggcaggtc gcggtcgagc cgggcgctgc caacgtcata
      361 ccggcgcgcg tcacagcact cgtcgatgcg agagcgcccg acgagaccac cctcgcggcc
      421 ctcgtcgacg cgatcacgga ggccggcgaa ggtgccggag cggaggtcga aaccttgttg
      481 cggacggctc cggtggagat ggacggcgac gtctgcgacg tcctccgccg ggcgctcgaa
      541 gagttggggc tcgaaccgcg ctaccttccg tccggcgccg gccacgacgc cgcggtggtt
      601 gccagggccg gcgtgcccag cggcatgctg ttcgtgcgca gccgcaacga cggtgccagc
      661 cactcgccgg cggagcacag cgagtccgag gatgtcaccc tcggcgtgga agcgcttgcg
      721 aaggccctcg cgcggctcgc tactgctcgg tgatgacgat ctcctgcgcg ccttcccaaa
      781 ggaagcgccg acccagctcc ggcaggttct ctgcgccgtc gaggatcgtc gcgaagtggt
      841 tcccggccag ctggcccgcc ttcgaggcga agttgacgta gccgtaggcc agcaggatct
      901 ccgtctcgct cacgccgccc ggatagagga cgatctcgcc cggatgcgga tagcacgtcg
      961 cgttctccgg gccgatcccg agatcgagct ccccaaacgg gatccagccc gcctcgccac
     1021 tccagcggac gtggatgatc cgcgactcga gcggcagcag gcgtcggaag gcagcgacgg
     1081 tctgtggagc gagctcctcc tctagctgcg cacggaacgc gaagcctccg gcgtggatct
     1141 cgagcattgc gcaaccctac ccagcttcgt tcgccggctg agcctccggg cccggccgct
     1201 actcgctcgg accgccctga tccgcccagg cgacgacgag atcgcgctcc tcgtccgtca
     1261 tccgggtgat gttgccgagt ggcatcgtcc ccgcgcgaac cacggcgccc acctcgtccg
     1321 cctgggcgac gatctggtcg ctcgtctcca agacgatgcc cgcgggcggg ctcgagaacc
     1381 cgtcctgcgt cggcgcgagg gcatgacacg tcgcgcaacg ctgcgcgatg atcggctcga
     1441 cctcggccag agacaccgcg ttctggccct tcgcgtccga cgtcgagttg ccgggctgca
     1501 ggacgaaggc gagtgcgatc aacccggcga cgccgacagc cgggatccac cagagagtcc
     1561 tgccgccgtg gcgaaggttg aagaacacgc gggccagcac gccgaccgcc atcagcgcca
     1621 ccagcacgag ccagtcgtag gacgccgcgt agaggaacga gaaatggccg gcgagcatcg
     1681 tcagcagcac cggcagcgtc aggtagttgt tgtggaccga gcgccgcttc gcctcgatcc
     1741 cgggagctgg atcgggctcg cgacccgcct gcttcgcccg gacgagctcc cgatgagcgg
     1801 ggatgatcga gaacaggacg ttggcggcca tgatcgtccc gagcatcgcg cccacttgga
     1861 gagaggccgc acgcgggctg aagagctggg tcgcgatcca cgcggacgcc acagccgcca
     1921 gagcgatcag cacgcccagc actcgatcgt ccttcacgaa gcggcagagc acctcgtaca
     1981 cgaccagggc ggctactaga aggccgacgc tgagtcctat cgcggcccat ggctcgagat
     2041 ccgcgactgc cggatcgatc aggaacgttc gcgcgtcgag gtagtagagg acgatcatca
     2101 gggcgaagcc ggaaagccag gtcgtgtacg cctcccactt gaaccacgcg aggtgatccg
     2161 gcagcacccg tggcgcgacc tggtacttct gcacctggta gaagccgccg ccgtgcacct
     2221 cccacagctc gcctgcaacg cccgcgtccg cgtcgtcctt gcgcttcggt gggcgcagcg
     2281 actggtcgag cagcacgaag tagaaggatg tgccgatcca ggcgattgcg gcgatcacgt
     2341 ggagccagcg gagcagcagg tccagccagt cacggatata ggggtcgagc accgccgcca
     2401 gcgtcgtcat ccgcgtcgcc accgatcctc ggcgatggcg acgagctcac cgagagcggt
     2461 ttccagctcc tcctccgtcg ggcgctctat acgccgacgc agcaccgcga ggatctccgt
     2521 cttcggacgg cggttcacga agacgacgaa acggaacccg tgcgttctct cgtagcgtcg
     2581 gttgagccga tcgagttcct cgagtaccgc gggctccgca tccgctccct gctccacggc
     2641 cgaacgagcc gacagggccg tgggctgtcc gattgcgggg tgtgcgtcga ggatctcctt
     2701 cttctcgtcc tccgacagct cgtgcaccag cgtgcgagcc cggcgcaacg gatcgtcttc
     2761 tccggccagg cgctcgacga agcgagttcg cccctcgaac agctcagcta gctcgtccgc
     2821 gctcagctgc cgcggtagct cgcgcatccg tagggcgaga cgagaagcgg gacgtggtag
     2881 tggtcgtctg ccagctcgac ctcgaggctg atgcgccgga agaacggtga gggtggacgg
     2941 aagacgagct ggtagacgcc cggctcgagc ccaccggcga gctcggcgat tcgtccatca
     3001 gcgtcggttt ccgcccacgc cacgagatcc tccgcttgga acagctcgac gcgtgtcccc
     3061 gccaccgggc ggcccgccgc ggtgtcgaga acgtgggtcg agagagagcc gctcacaccg
     3121 tgcatcttcg cggctgcatc agatgaccgc gggcgccggt cggcccgtcc gcctgcagca
     3181 gtgtctcccg gcccggggcc gggtgtgcct tgctgctcat gcctcgagcg gaacgatctc
     3241 gaagcggtcg acgtccacca gtccgtggtc ggtgagcttg aggtgcggaa tcaccgacag
     3301 cgcgagaaac gcgagcgact ggaacggcgc gtcgagcgtg cagccgagcg cacgcgccgc
     3361 cgccacacag gcgcggctcc gctcgatcac gtcgctatgc ggtagatccg agaggagccc
     3421 ggcaaccggc aggggcagct cagcgcgtat cccgtcgccg tccgcgacga cgaggccgcc
     3481 gcccagggcg gcgatgcgtt cgacggcggc gcgcatgctc gtgtcgtcca tcccggcgac
     3541 gacgacgttg tgcgcgtcgt gcgaaaagct cgaggcgagc gcgcccgagc gaagcccgag
     3601 gccgcgcgcg agtccgacgc cgatgcgtcc ggttccgagg tgacgctcga gcaccgcgac
     3661 cttcgcgatg tcgtgggccg cgtcggccat cacctcgccg tcggcgagcg ttggcctttc
     3721 cgtgagcagg caggtcgtga tctgccccgg gacgattccg attacccgag ccaggccgtc
     3781 gccgccgcgc caggggagac ggaacgtgtc gtcgtcgatc ggtgcgagcc gaaccgtctg
     3841 gcgcagccag gacggcacgg gcggatgcga gaagtcgcct accggcgccc cggacttgag
     3901 cacgacttcg ggtacgaagc cctccagatc cggcagcaga aggatgtccg cgcggtagcc
     3961 gggtgcgagg cccccgcgcc gctgcagtcc gtgccagcgc gccgggttga gcgaggcaag
     4021 cacgatcgcg tcctccggcg gcaggccgag gcgaaccgct tcccggacga tcgcgttcat
     4081 atgaccatcg tcggcgatgt gctccggctc gcggtcgtcg gtgcagaaag cgattcgatc
     4141 cgtgccgtac tcccgaacga gcggaacgag cgcggcgaga ttccgggccg ccgacgcctc
     4201 ccggatcagt agccacatgc ccgcgcggag acgctctcgt ccctcctcga ccgtcgacgc
     4261 ctcgtgatcc gacccgatac cggccgcagc gtaggcctgg agctcaaggc cgagtacgcc
     4321 gggcgcgtga ccgtcgacgt gatcgacgcc tgcgatcgtg agcttcgcga gctcgtgatc
     4381 ggatcccgcg atcactcccg ggaagttcat catctcggcg agtccgacca tccgtcggcg
     4441 tcgcaacagg ctttcgaggt cgccgggctc gagcggccgc cgcggcgact cgaaacgcga
     4501 tgccgggacg cacgaggacg cagtgaaata gacgtccagc ggtaggccgg agctcgcatc
     4561 gagcagccag tggatgccgt cggtgcccag gacgttggcg atctcgtgcg gatccgcgac
     4621 caccgtggtc gtgcccaggg gcaacacgag gcgcgcgaac tcgtccacga gcagcttgga
     4681 tgtctcgagg tgcatgtgcg cgtcgacgag gcccggcacg acgtaccggt ccgtcgcgtc
     4741 gaggacgttt tcggcgtcgt agccgccgag gccagcgatg acgccgtcca cgaccgcgac
     4801 gtccacgtcc agccattcgc gggtgaagac cgagagaaca cggccgcccc ggacgacgag
     4861 atcggccggc tcgtcaccgc gggcgacggc gaggcgtcgg cgcaggcggt ccacgacccg
     4921 caacgtacta cgcttccgcc ggaaatggag gttctctccc cggcatccct ggacgaagcg
     4981 ctgcggctgc gctccgcgca cccggacgcg ctcgtgatcc agggtggcac ggacgtgatg
     5041 gtcgagctga acttcggccg ggcgcggccc gacgtcatcc tcaatctgaa cggcgttctg
     5101 gagctgcggg gctggcgcaa ggagaacgga acgctcgtgc tcggcgccgg tctgagctac
     5161 gcggcggcgg agaccgacct cgccggcgcg ctcccggcgc tggccgaggc ctcgcgcacg
     5221 gtcggctctc cacagattcg ctcgcgcggc acgatcgggg ggaacctcgg gacggcgagt
     5281 ccggcgggag atgcgcttcc gccgctgctc atcgaggacg ccgaggtcga gctcgcctcg
     5341 cttcg
//
LOCUS       DBNGNK010000187         7531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_319083,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000187 DBNGNK010000000
VERSION     DBNGNK010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_319083"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="AC150V_05440"
     CDS             <1..515
                     /locus_tag="AC150V_05440"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3762400.1"
                     /translation="RYCPRCAVQLETEAGRVVCTSCGFTAYANSAPTASALVEDTSGR
                     LLLARRAVEPHTGKWDLPGGFLDEGEHPLDGLRRELREEAGVEIEPGDLLGIWLDSYG
                     GGPDAVATLNLYWLARITAGRPVPADDVEELRWFAADALPSHDEMAFTNTVLVLEAWR
                     DRRGAPTKSR"
     gene            601..1641
                     /locus_tag="AC150V_05445"
     CDS             601..1641
                     /locus_tag="AC150V_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3762401.1"
                     /translation="MRLAVLSILRHAPGQRIVVSCPRPPDEFRLWLWSLPDVELYVDE
                     ALEGLGYDVKPRVLLQMLDLYGDTMWLDTDIVVTRDFRPLLRDADDRTLVVAEEVHLG
                     QEQGSDYRARAWGLEPGRPLPHTANTCVIRATAAHVELLKRWQELLSRPEYRQAQALQ
                     PAERPLYLLTDMEPFTALLGSREFASVPVVLLRDGRAIAQCYGSAGLTPADRLRATAR
                     GMPPFVHALGWVKPWEKASPKRGGAAGGGRMAPLRRSADLLHLELSPYAALARGYKDE
                     LNGDAAWLRIESPAARFLSFAFRGHPALQELPLSVADSLRQYLRARLGRGRFRLDWDE
                     EGAAARPLRGDE"
     gene            complement(1648..1965)
                     /gene="trxA"
                     /locus_tag="AC150V_05450"
     CDS             complement(1648..1965)
                     /gene="trxA"
                     /locus_tag="AC150V_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015069264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR3762402.1"
                     /translation="MATAVTESTFDQEVIKSDKPVIVDFWAEWCGPCHAVSPVLEKIA
                     EEREDEVKLVKVNIDEERGLADRYGIASIPTMILFKDGEPAAAVIGAQPKGAIERSLG
                     LAA"
     gene            2084..5137
                     /gene="ileS"
                     /locus_tag="AC150V_05455"
     CDS             2084..5137
                     /gene="ileS"
                     /locus_tag="AC150V_05455"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR3762403.1"
                     /translation="MFSQLPDKPDHPSIELDILDVWEREGTFAALREQNAGGPIFSFV
                     DGPVTANKALAVHTAWGRTLKDVFQRYKALRGFHQRYQNGFDCQGLWIEVGVERELGL
                     NSKREIEEYGLAEFARKCREVVVWSSEEITRGSVRLGMWMDWGNDYFTFSDTNIEYIW
                     RFLSVVHEKGWLYLGHRSTEWCPRCGTSISAHELHGHYEDRTDPSLYVRFPLLDRDGE
                     ALAVWTTTPWTLPANVAAAVRPDAEYGLLENGDWVAVALFPDETFMQRLRGSELVGLH
                     YRGPFDTLGPGASVEHRVIPWEDVDLATGTGIVHLAPGCGAEDFELSHEHDLAVLTPV
                     DESGRFYPEYGWLHGISTGEAAEQIVSDLGDRGLLVKAETIEHAFPFCWRCHTPLIFR
                     ISDDWFISVEEIRQPLLEANATVEWTPLYMGKRMDDWLRNMTDWNISRRRYYGLPLPF
                     YPCDCGHLNVIGSRRELEQRAVSGLDQLEELRRPWIDDVPITCEECGQKVRRIEEVGD
                     VWLDAGIVPFSTLGWQSEKWVPAGNGTGAAKGLTTADLPDHAHWEEWFPADWVSEMRE
                     QIRLWFYSQLFMAVALTGRSPFRQVLGYEKMLDEHGREMHGSWGNMIDAEDAFARMGA
                     DVMRWQYCAQPPDRNLLFGFGPAHEIQRKLLTLWNSAKVLVIYANVEDWTPSWGDLED
                     GPGGDLEPLDRWLVARTRRLVADATAGYERWLTVEVIRAFDDFVDDVSNWYIRRSRRR
                     FWDGDPVALATLWHALVQGLRVVSPVMPFLSEHLWRVLTEPCDGAPSSVFLAGWPEQP
                     EPDEALLAEVAEVRRVVELGRQARAVTGLKLRQPLRRLLVEGTPRAANHAGEIGDELR
                     VKEVEFGRVEATELRVKPNLPVLGPKLGKELGALRAALAAGEFEELDDGRFRAAGHEL
                     GPDEVLVERTGREGWAVAAEDGVTVALDAHVDDELAQEGRVYELIHQVNSMRKDAGLE
                     LTDRIRLTIPAADSDLLAHAEWIKAETLAVELDADGGGEPVIAKA"
     gene            complement(5224..6213)
                     /locus_tag="AC150V_05460"
     CDS             complement(5224..6213)
                     /locus_tag="AC150V_05460"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3762404.1"
                     /translation="MRLEAVKGYDQVVMVERVLLASPRGYCAGVERAVETVELALEHY
                     GAPVYVRKQIVHNIHVVRELEARGAIFVDEETEVPAGSTLVYSAHGVAPSVRENSARL
                     RHTVIDATCPLVTKVHAQASRYAKAGYTILLIGHEGHEEVEGTLGEAPNQTILVEDAQ
                     QAETVQPPSAAQLAYVTQTTLSVDETSEIIGVLRRRFPDIVGPQKEDICYATSNRQWA
                     VKDMLPEIELLLVIGSRNSSNSNRLVEVARDAGVASYLLDDETEIDERWLEGVTTVGI
                     TSGASAPEVLVTRVCDWFRARGVDDIEPFAMVDEDVFFRLPVELRRELALVDS"
     gene            complement(6219..>7531)
                     /locus_tag="AC150V_05465"
     CDS             complement(6219..>7531)
                     /locus_tag="AC150V_05465"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3762405.1"
                     /translation="PNRAYFTARLGDELGAGDEPQLAVMLIDLDRFKEVNDTLGHGNG
                     DLLLQELGARLTDAMDETDFVARLGGDEFGVVSTGTGDAASAIGLAGRLRATIAEPVD
                     VGGVSLEVQASVGIALAPQHGSDGETLLRRADIAMYAAKPGDAPMVYRPDLDDNSPLR
                     LALAAELRRALERQELVVYYQPQVELGSGAVTTVEALVRWRHPERGFLSPDEFLPIAE
                     QAGLMRPLTRAVLEDALRQCRAWHNAGRRLEVAVNVSGRDIVDLRLPQEVARALAEHG
                     IDPCYLQLEISEGAVLGDNQRGTVVIEQLAALGVRIAIDDFGVGYSSLGQLRRLPVAV
                     LKIDKSFVQAMTDERTGIVIVRSTIDLAHGLGLEVVAEGVEDEATLDRLADAHCDLAQ
                     GYAISRPLPADDLTAWLEAVPASKTPGDVVPLHQSVGRAAAETA"
BASE COUNT         1163 a         2567 c         2574 g         1227 t
ORIGIN      
        1 ggcgatactg cccgcgctgc gccgttcagc tcgagacaga ggccggacgc gtcgtctgta
       61 cgtcgtgcgg cttcacggcg tacgcgaact ccgcgccgac tgcctcggcg ctcgtcgagg
      121 acacgagcgg ccgcctgctc ctcgcgcggc gcgcggtcga gcctcatacc ggcaagtggg
      181 accttccggg aggctttctc gacgaggggg agcacccgct cgatgggcta cgccgcgagc
      241 tgcgcgagga ggcgggcgtg gagatcgagc cgggcgatct gctcggcatc tggctcgact
      301 cctacggcgg cggacccgat gcggtcgcca ccctgaacct ctactggctc gctcgcatca
      361 cggccggccg gcccgtaccg gcggacgacg tcgaggagct gcggtggttc gcggccgacg
      421 cgctaccgtc gcacgacgag atggcgttca cgaacacggt tctggtgctc gaggcctggc
      481 gcgaccgccg cggcgcacct acaaagagcc ggtgaggcac gtattgcaag ggcgatcaag
      541 ccgggcggtg gggcgctgat cgtctgtctc cacgaggaca ggccccacgc gctcgtcggc
      601 gtgcggctcg ccgtcctgag catcctccgc catgcgcccg gacagcggat tgtcgtcagc
      661 tgcccccgtc cgcccgacga gttccggctt tggctgtggt cgttgccgga cgtcgagctc
      721 tacgtggacg aggcgctcga aggactcggc tacgacgtga agccacgggt gctgctgcag
      781 atgctggacc tctacggaga cacgatgtgg ctggacaccg acatcgtcgt cacgcgggac
      841 ttccggcctc ttctccggga cgccgacgat cggacgctcg tggtcgccga agaggtgcat
      901 ctggggcagg agcaggggag cgactaccga gcgcgcgcct ggggtctcga gcccggccgg
      961 cccctgcccc acacagcgaa cacctgcgtc atacgtgcta cagctgccca tgtcgagctg
     1021 ctgaagcgct ggcaggagct gctgagcaga ccggagtacc ggcaggccca ggcactgcag
     1081 cccgcagagc ggccgctcta cctcctcacc gacatggagc ccttcacggc gctgctgggc
     1141 tcccgcgagt tcgcgtccgt accggtcgtt cttctgcggg atgggcgcgc gatcgctcag
     1201 tgctacgggt cggcgggact gactccggcc gatcggctcc gagcgaccgc acgtggcatg
     1261 ccgcccttcg ttcacgcgct cggttgggtc aagccctggg agaaggcgag cccgaaacgc
     1321 ggtggcgctg cgggtggcgg gcgaatggcg ccgctgcgcc gctccgccga cctcctccac
     1381 ctcgagctct cgccctacgc ggcgctcgcc cgcggctaca aggacgagct gaacggagac
     1441 gccgcctggc tgcgcatcga atcaccggcc gcgcggtttc tctccttcgc ctttcgaggg
     1501 catcccgccc tgcaggagct gcccctgtcg gtcgccgact cgctcaggca gtacctgcga
     1561 gcccggttag gccggggcag gttccggctc gactgggacg aggagggcgc ggccgcacgc
     1621 ccgttgcgcg gggacgagtg aggctgctca ggctgccagg ccgagcgagc gttcgatggc
     1681 gcccttcggc tgcgcgccga tgacggcagc ggcgggctca ccgtccttga agaggatcat
     1741 cgtcgggatc gaggcgatgc cgtaacggtc tgcaagtccg cgttcctcgt cgatgttgac
     1801 cttcaccagc ttgacctcgt cctcgcgctc ctcggcgatc ttctcgagca ccggtgagac
     1861 ggcgtgacag gggccgcacc actcggccca gaagtccacg atcactggct tgtcgctctt
     1921 gatcacttcc tggtcgaacg tcgactcggt gacagcggtt gccatgcgtt cctttcgtag
     1981 gctttgactc gggttacaga gatgtgaacg acggaggccg taaacctctt cccggcgcct
     2041 cgtgacggga gttgccctcg gaacggagca ctaagatcgc cgcatgttct cacaactccc
     2101 ggacaagccc gaccaccctt cgatcgagct cgacatcctc gacgtctggg agcgcgaggg
     2161 gacgttcgca gcgctccggg agcagaacgc cggcggcccg atcttcagct tcgtcgacgg
     2221 gccggtaacc gcgaacaagg cactcgccgt ccataccgcg tggggtcgca cgctcaagga
     2281 cgtcttccag cgctacaagg cgctgcgcgg cttccaccag cgctaccaga acgggttcga
     2341 ctgccagggg ctgtggatcg aggtcggcgt cgagcgcgag ctcgggctga actcgaagcg
     2401 cgagatcgag gagtacgggc tggccgagtt cgcgcggaag tgccgtgaag tcgtcgtctg
     2461 gtcgtcggaa gagatcaccc gaggctcggt gcgcctcggc atgtggatgg actgggggaa
     2521 cgactacttc accttctccg acacgaacat cgagtacatc tggcggtttc tctcggtcgt
     2581 ccacgagaag ggctggctct acctcggcca tcgttcgacc gagtggtgtc cccgctgcgg
     2641 cacgtcgatc tccgcccatg agctgcacgg ccactacgag gaccgaaccg atccgtcgct
     2701 ctacgtccgc ttcccgctcc tcgaccgcga tggggaggcg ctcgctgtct ggacgaccac
     2761 gccctggact ctgcccgcca atgttgccgc cgcagtccgg ccggacgcgg agtacgggct
     2821 gctcgagaac ggtgactggg tcgccgtcgc gctcttcccg gacgagacgt tcatgcagcg
     2881 tctgcgcggc tcggagctcg tcgggctcca ctaccgcggg ccgttcgaca cgctcgggcc
     2941 gggcgcgtcc gtcgagcatc gcgtgattcc ctgggaggac gtcgacctcg caaccggcac
     3001 cgggatcgtc catctcgcgc ccggctgcgg cgccgaggac ttcgagctct cgcatgagca
     3061 cgaccttgcc gtcctgacgc cggtggacga gagcgggcgc ttctatcccg agtacggctg
     3121 gctgcacggc atctcgacgg gggaagcggc cgagcagatc gtctccgacc tgggcgaccg
     3181 cgggctcctg gtcaaggcag agacgatcga gcacgcgttc cccttctgct ggcgctgcca
     3241 cacgccgctc atcttccgca tctcggacga ctggttcatc tcggtcgagg agatccgcca
     3301 gccgctgctc gaagccaacg cgacggtcga gtggacacct ctctacatgg gcaagcgcat
     3361 ggacgactgg ctgcgcaaca tgaccgactg gaacatctca cggcgacgct actacggcct
     3421 gccgctgccc ttctatccct gcgactgcgg gcatctgaac gtgatcggct cgcgccgaga
     3481 gctcgagcaa cgagccgtct ccgggctcga ccagctcgag gagctgcgcc ggccgtggat
     3541 cgacgacgtg ccgatcacct gcgaggagtg cgggcagaag gtgcgccgga tcgaggaggt
     3601 cggcgacgtc tggctcgacg cggggatcgt ccccttctcg acattgggct ggcagagcga
     3661 gaagtgggtg ccggcgggca acgggaccgg agccgcgaag gggctcacca cagccgacct
     3721 gcccgaccac gcccactggg aggagtggtt cccggccgac tgggtctccg agatgcgcga
     3781 gcagatccga ctctggttct actcgcagct cttcatggcc gtcgctctga cgggtcgctc
     3841 gccgtttcgg caggtactcg gctacgagaa gatgctcgac gagcacggcc gtgagatgca
     3901 tggctcctgg gggaacatga tcgacgccga ggacgcgttc gcgcggatgg gcgccgacgt
     3961 gatgcgctgg cagtactgcg cccagccacc cgaccgcaac ctcctgttcg gcttcggccc
     4021 ggcccacgag atccagcgga agctcctgac gctctggaac tcggccaagg tgctggtcat
     4081 ctacgcgaac gtcgaggact ggacaccgag ctggggcgac ctggaggacg ggcccggcgg
     4141 cgacctcgag cccctcgatc gctggctcgt cgccaggacg aggcgactcg tcgccgacgc
     4201 gacggccggc tacgagcgtt ggctcaccgt cgaggtgatc cgcgccttcg acgacttcgt
     4261 ggacgacgtg tcgaactggt acatccgccg ctcgcggcgg cgcttctggg acggcgaccc
     4321 tgtcgcgctc gcgacgttgt ggcacgcgct cgtccagggc ctccgtgttg tttcgcccgt
     4381 gatgccgttc ctaagcgagc atctgtggcg cgtccttaca gagccttgcg acggtgcgcc
     4441 gtcatcggtc ttccttgccg gctggccgga gcagccggag cccgacgagg cgctcctcgc
     4501 cgaggtcgca gaggtccgcc gcgtcgtcga gctcggccgc caggcgcgcg cggtgacagg
     4561 gctgaagctg cgccagccgc tccgtcgcct cctcgtcgag ggtacaccgc gggctgccaa
     4621 tcacgcgggc gagatcgggg acgagctacg cgtcaaggag gtcgagttcg gccgcgtgga
     4681 ggcaaccgag ctccgtgtca agccgaacct gcccgttctc gggccgaagc tcggcaagga
     4741 gctcggcgct ctgcgggctg cgctcgcggc gggggagttc gaggagctgg acgacgggcg
     4801 cttccgcgcc gccggccacg agctgggccc cgacgaggtg ctggtcgagc gcacgggccg
     4861 cgagggctgg gccgtcgccg cggaggacgg ggtcaccgtc gccctcgacg cgcatgtcga
     4921 cgacgagctc gcacaggagg gccgcgtcta cgagttgatt caccaggtga actcgatgcg
     4981 caaggacgcc gggctcgaac tgacagaccg gatccggctc acgattcccg ccgccgactc
     5041 cgacctgctg gcgcacgccg agtggatcaa ggccgagacg ctcgcggtcg agctcgacgc
     5101 cgacggcggg ggcgagcccg tgatcgcgaa ggcctagatc gctgtgccgg aatcaggcaa
     5161 gtaacagact gttacttgag gtggcgccgg gctttgcctc ttgcgtcttt tgacagccgg
     5221 ccgtcaggaa tcgacgagcg cgagctcacg gcgcaactcc accgggagcc ggaagaagac
     5281 gtcctcgtcg accatcgcga acggctcgat gtcgtcgacg ccacgagcac gaaaccagtc
     5341 gcagacccgc gtgacgagca cctcaggcgc cgacgcgccc gaggtgatgc cgaccgtggt
     5401 gacgccctcc agccagcgct cgtcgatctc cgtctcgtcg tcgagcaggt acgaggcgac
     5461 gcccgcgtcg cgggccacct ccaccagacg gttcgagttc gacgagttgc gcgagccgat
     5521 caccagcaga agctcgatct ccggcagcat gtccttcacc gcccactgac ggttggacgt
     5581 cgcgtagcag atgtcctctt tctgcggccc tacgatgtcg ggaaagcggc ggcgcagaac
     5641 accaatgatc tccgacgtct cgtcgacgga cagcgtcgtc tgtgtcacgt atgcgagctg
     5701 cgcggcggag ggcggctgca ccgtctccgc ctgctgggcg tcctcgacca ggatcgtctg
     5761 gttcggcgcc tcgccgagcg tgccctcgac ctcctcgtgc ccctcgtgac cgatgagcag
     5821 aatcgtgtac cccgccttgg cgtagcggct cgcctgcgcg tggactttcg tgacaagggg
     5881 gcaggtggcg tcgatcaccg tgtggcgcaa gcgtgcggag ttctcgcgca ccgatggcgc
     5941 gacgccgtgg gcggagtaca cgagcgtcga gccggcaggc acctctgtct cctcgtcgac
     6001 gaagatcgct cctcgcgcct cgagctcgcg cacgacgtgg atgttgtgga cgatctgctt
     6061 ccgcacgtac acgggtgcgc catagtgctc gagcgcaagc tcgactgttt cgacggcgcg
     6121 ctcgacccct gcgcaatagc cacgcggcga ggcgagtaga actcgctcga ccatcaccac
     6181 ctgatcgtag cccttcacgg cctccaggcg catcgccgtc aggctgtttc ggctgcggcg
     6241 cgacccacgc tctggtgaag cggtaccacg tcacccggcg tcttggatgc gggcaccgct
     6301 tccagccagg ccgtgagatc gtcggcaggc agggggcgcg atatggcgta gccctgagcg
     6361 agatcgcagt gagcgtccgc caaccggtcg agcgtcgctt cgtcctcgac cccttcggcg
     6421 acgacctcga ggccgaggcc gtgtgcgagg tcgatcgtcg agcgcacgat cacgattccg
     6481 gtgcgctcgt cagtcatcgc ctggacgaac gacttgtcga tcttgagcac tgcgaccgga
     6541 agccgtctga gctggccgag ggaggagtat ccgacgccga agtcgtcgat cgcgatccgc
     6601 acgccgaggg ctgcaagctg ctcgatcacg accgtcccac gctggttgtc gccgagaacc
     6661 gctccctccg agatctcgag ctgcagatag cagggatcga ttccgtgctc ggcgagggcg
     6721 cgcgccacct cctgcgggag gcggaggtcg acgatgtccc gccccgagac gttgacggcg
     6781 acctcgaggc ggcgccccgc gttgtgccag gcgcgacact gccgcagggc gtcttccagc
     6841 accgcccgcg tgagcggccg catcagtccg gcctgctcgg caatgggaag gaactcgtcg
     6901 ggggagagga agccacgctc cgggtggcgc cagcgaacga gcgcctctac ggtcgtgacc
     6961 gcgcccgagc cgagctcgac ttgtggctgg tagtagacca ccagctcctg cctctcgaga
     7021 gcgcggcgca gctcggccgc aagtgccagt cgaagcggcg agttgtcgtc gagatcaggc
     7081 cggtagacca tcggggcgtc tcccggtttc gccgcgtaca tggcgatgtc ggctcgtcgg
     7141 agaagcgtct ccccgtcgct gccgtgctgc ggagccagtg caatcccgac gcttgcctgc
     7201 acctcgagcg agacgccgcc tacgtcgacg ggctccgcga tcgtggcgcg aagccgaccg
     7261 gccagcccga tggccgacgc tgcgtcccct gtcccggtag acacgactcc gaactcgtcg
     7321 ccgccgagtc gtgcgacgaa gtcggtttca tccatcgcgt ctgtcagtcg ggcgccgagc
     7381 tcctggagca ggaggtcacc gttaccgtgc ccgagcgtgt cgttgacctc cttgaagcgg
     7441 tcgaggtcga tgagcatcac agcgagctgc ggctcgtctc cggcaccgag ctcgtcgccc
     7501 agacgagcgg tgaaatacgc ccggttcggc a
//
LOCUS       DBNGNK010000188         9060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_964008,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000188 DBNGNK010000000
VERSION     DBNGNK010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9060
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_964008"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1087
                     /locus_tag="AC150V_05470"
     CDS             185..1087
                     /locus_tag="AC150V_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3762406.1"
                     /translation="MRRPTSVELMLLATIVLWSLNLTVSRYVLTHGFQPLSYAVLRYA
                     FAALIFLVLVLAAEGSLRVRRSDALLVLLAALVIWLNQLAFVYALDTGAASTLALLLG
                     AIPIIAALLGLVLGTERVTARFWGGAALSFVGVALVAAGAGARLSGGLGAVLLGLATA
                     ATWAVYSVTIRPLMRRYSPYRISALVLSLACLPLAASAWPQAASQDWALGWEIWALFL
                     FATIGPLVVTNVFWFRSLHRIGPSRATLAVNLQPFVAAVFAVVLLSERMTWLQVLGGV
                     LIGLGIFLARRRRRPQPAASSSSA"
     gene            complement(1057..4368)
                     /locus_tag="AC150V_05475"
     CDS             complement(1057..4368)
                     /locus_tag="AC150V_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3762407.1"
                     /translation="MPTCATCGQDNPDGFAFCGRCGARLADAPAPREERKVVTVLYAD
                     MVGSTARADELDPEDVRAVLAPFHASLRDQLAGFGGTVEKFIGDAVVAVFGAPVAHED
                     DPERAVRAALRIQETIADLNAEDPELHLEVRIGVNTGEALVALDARPEAGEGMVSGDV
                     MNTGARIQAAAPPGSVLVGEATYRATEHVVSYETAAPIRAKGKTEPVAVWRAIEPRAR
                     LGLDVRQGAPAPLVGRKRELELVLGALGRSQSERETQLVTLVGVPGIGKSRLVYETLR
                     VVDAAPELVWWRQGRCLPYGEGVTFWALSEMMKAQTGVLDTDSSADAERKLRETLEDV
                     APEGADRLEPHLRPLVGLVREGVGESAESFAAWREVFEALADVRPLVLVFEDLHWADD
                     GLLDFVDHLADWARGVPIFVLATARPELLERRPGWGGGKLNATTLALSPLSDGETAEL
                     VAALADRAALPDEVREAVLARAAGNPLYAEQYVRMLADRAGDPSALELPETVQGIIAA
                     RLDALAPDEKALLQEAAVVGKVFWRGALVALGRATEGIELRLHGLERKDIVRRERRSS
                     IGGETEYAFRHALLRDVAYGQIPRGTRSVLHSRTAEWIETLSERVEDRAEMLAYHWGE
                     AASYARQAGIDTAGLDERAREALTDAAERAESLSAFQAAARFHGRAAELWPEGSPQRT
                     LAAARRLIALWAARVPLTEGAELVEPLVAAGRVEEAALVHAGLGLGAWNEARRDEAYE
                     HAEQAAALIRDRPASTAKVSVLATQARLEFIQGRGDRGVALAKSALVVADEIGADRQR
                     AEVLVTLGSAQVANGHVTDGLAALERGIELARQEHPVEELRGRNNLGSYFAAFGRLSD
                     AAEQRASALTGSERLGVSFYTRWQQVERIFHDYWSGNWERALDTANGVLALAEAGDTH
                     LMDVGARWTRSALALARGDLVAPRREADLALEFARDSADPQTLAPLLAWHARFLAETG
                     DAEGATVEAEEFLSSVSTDSIEFWASSIAATLDLLGRGESFPSDPPHDAFRPWWDAGA
                     ALAGGSYHEAADRYREIGSLPDEAWARLRAGRALMTEGRHADATAELERSLAFWRSVG
                     ATRYVRQAEELLAAG"
     gene            4395..5741
                     /locus_tag="AC150V_05480"
     CDS             4395..5741
                     /locus_tag="AC150V_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR3762408.1"
                     /translation="MLPQRRTALVSVGAAVALMAIKLVTGLGSGSLGLVAEALHSGTD
                     LVAALLTFYAVGYAVKPADVGHPYGHGKAEHLSALAEAAVLALVSIGVAALAVGRLAG
                     WLETEVDAAWWVFAAIAAVIMIDVSRTIVSLRTARRFRSDALLSNAVHFAGDIAGTLA
                     VLAGLLAVRAGWPEGDSIAALLVAALVLVAAARLMRRNVNVLMDRAPADAVAAARQAL
                     RDVEPAVDVRRLRLRQAAGRVFADVVIRVPPDEAVAQGHAAADSVEEALHVVLPGSDV
                     VVHVEPEGGDAVLRERVRAAAMTVPGVREIHNLTVIDLDDGVTEASLHLKLPGQLSLD
                     EAHDLAEAVEQAIVDAVPEVSAVQTHLEPLDEEAAGREVTRVPADVAELVAAATGRAP
                     REARLLRTGDGLVLFLTLVVDGGATLAEAHEEAGAVAAQIRDAHPEIAEVIVHTEP"
     gene            5758..6438
                     /locus_tag="AC150V_05485"
     CDS             5758..6438
                     /locus_tag="AC150V_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762409.1"
                     /translation="MRLCMFHPLDRPMERGWVGRVDGDRVIHLAAQTLQSFFIGGGGA
                     REHADYALDGVAFLAPVLHPPTVRLFDSQDTFSFANATAIAGPGAGVNGGRAPVVLPR
                     IAAVIEGEQRPGGFTVLAEFRAPDRPVPKDRDFALVLGPVVVTPDELEPDGVEVAVRS
                     GETERLRAVAPPFDWHDALRLAALGTKLRPGDIVVGPAAGEVAVGPGSIELGVARIGI
                     LGAEVRDG"
     gene            complement(6448..7539)
                     /gene="hppD"
                     /locus_tag="AC150V_05490"
     CDS             complement(6448..7539)
                     /gene="hppD"
                     /locus_tag="AC150V_05490"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HIR3762410.1"
                     /translation="MSDFMPLDGWDHVELWVGNAKQAAFFYEHALGFSPVAYAGPETG
                     VRDRASYVLEQGEIRFVVTSGLRADSEISRFAAVHGDAVKDVALLVSDSAEAYRQAVQ
                     RGARGITEPYTLDDDYGRVRLSAIGTYGEVVHTFVERSDYGGPYLPGYVSLARNGVGH
                     DGVGLVAIDHVVGNVELGRMDEWVDYYARVLGFENIIHFSDEIQTEYSALMSKVMSDG
                     ERKIKFPINEPAEGKKKSQIEEYLEFNSGPGAQHVAMQTTDIVRTVEGLAKRGLPLLR
                     TPHTYYEDLPDRIGEISEDYADLERLGILADRDEDGYLLQIFTKNAQDRPTLFFEIIE
                     RHGATGFGEGNFKALFQAIEREQALRGNL"
     gene            7805..8410
                     /locus_tag="AC150V_05495"
     CDS             7805..8410
                     /locus_tag="AC150V_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HIR3762411.1"
                     /translation="MRLVLDWDGTVTEVDTLHLVIEQFGDLGVFRAMEGELERLTLHD
                     IIGAEMQTITAPLDEVVAWLLENARIRPGFVELVDAHEPTIVSAGFHELIEPLLAQAG
                     VEVPVIANRIVSAAASGWRVEFRDTAVCEFCGEPCKRAAAAADGAFAYVGDGVSDRCA
                     SLAADRIFARAGLARYLDDAGVPYEPFADLRDVADALAAPI"
     gene            8536..>9060
                     /locus_tag="AC150V_05500"
     CDS             8536..>9060
                     /locus_tag="AC150V_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762412.1"
                     /translation="MNKGTLIAALVVLGALLVPVTAAAAKHEVSGPLTVAGKGFFRGE
                     LEMADPGDSRPVKIAGGRGYVGVLDLAGDLRVRCHGRGVAKKKETDRGTVYLCKGARG
                     IAELVGSHFRFRGFAKRFVIHLPDGTTGKLAGRLIRPGTANDRPPRGERPDAPAADAP
                     ADGAAGDAAPSDEPV"
BASE COUNT         1200 a         3159 c         3231 g         1470 t
ORIGIN      
        1 gggccatctt ttcctcgccg tagcggcgaa tggcgttgtc cgcggggagg tcgcggatga
       61 gctcttcgac gcgcgcgcgg agctggtatg cgaagtgcgg cgtcgcgggc ccgacgaggg
      121 cgtcgacgtc ttcgatcgtc ggctcggcca ttcgggcgca agggtaacca gtagcgtgcg
      181 ccagatgcgg cggccgacga gcgtggagct gatgctcctc gcgacaatcg tgctctggtc
      241 gctgaacctg acggtgagcc gttacgtcct cacccacggg tttcaaccgc tctcgtacgc
      301 ggttctccgc tacgcgttcg ccgcgctgat cttcctcgtc ctcgtgctgg cggccgaggg
      361 ctcgctccgg gtgcgtcggt cagacgcgct gctcgtcctg ctggcggcgc tcgtcatctg
      421 gctcaaccag ctcgcgttcg tctacgcact ggacaccgga gcggcttcga cgctcgctct
      481 cctcctcggc gcgatcccga tcatcgctgc gctgctcggg ctcgtcttgg ggacggagcg
      541 ggtgacggcg cggttctggg gcggcgccgc gctgtcgttc gtgggcgtgg cgctcgtcgc
      601 tgcgggtgcc ggcgcccgcc tgagcggagg gctcggcgca gttctgctcg gcctcgccac
      661 ggctgcgacc tgggcggtct actcggtcac gatcaggccg ctgatgcgcc gctactcgcc
      721 ctaccggatc agcgcactgg tgctctcgct cgcgtgcctt ccgctggcgg cgtccgcctg
      781 gccgcaggcg gcgtcgcagg actgggcact cgggtgggag atctgggcgc tcttcctctt
      841 cgccaccatc ggcccgctgg tggtgacgaa cgtgttctgg tttcggtcgc tgcaccggat
      901 cggcccgtcg cgcgcaacgc tcgcggtcaa cctgcagccg ttcgtcgcgg cggtgttcgc
      961 ggtcgtcctt ctgtcggagc ggatgacctg gctccaggtg ctcggcggcg tgctgatcgg
     1021 gctcgggatc ttcctggcgc ggcggcgtcg gcgacctcag ccggctgcga gcagctcctc
     1081 tgcttgacgg acgtagcggg tcgctccgac ggaacgccag aaggcgaggg agcgctccag
     1141 ctcggcggtc gcatccgcgt gtcgtccttc cgtcatgagc gcacgtccgg cgcgcaggcg
     1201 ggcccaggcc tcgtcgggca gcgagccgat ctcgcggtag cggtcggccg cctcgtggta
     1261 gctaccgccc gccagtgccg ctcccgcatc ccaccagggg cggaaagcgt cgtggggtgg
     1321 gtcggacgga aaggactcac cgcggccgag cagatcgagc gtggcggcga tgctggaggc
     1381 ccagaactcg atcgagtcgg tcgacacgga ggagagaaac tcctccgctt ccactgtggc
     1441 accctccgcg tcgccggtct ccgcgaggaa gcgggcgtgc caggccagaa gcggagcgag
     1501 ggtctggggg tcggcgctgt cgcgggcgaa ttcgagagcc aggtcggctt cacgccgcgg
     1561 agcgacgagg tcgccccggg cgagtgccag cgcgctccgt gtccatcgcg cccctacatc
     1621 catcaggtgg gtgtcgccgg cttctgcgag tgcgagaacg ccgtttgcag tatcgagcgc
     1681 gcgctcccag ttgccgctcc agtagtcgtg gaagatcctc tcgacctgct gccaccgggt
     1741 gtagaaggac accccgagcc gctcggagcc ggtgagcgcc gaggcgcgct gctcggccgc
     1801 atcgctcaac cgcccgaacg ccgcgaagta cgagccgagg ttgttccgac cgcgcagttc
     1861 ctcgacggga tgctcctgcc gggcgagctc gattccccgt tcgagcgccg ccagaccgtc
     1921 ggtcacgtgt ccgttcgcga cctgtgccga gccgagtgtg acgagcactt ccgcgcgctg
     1981 gcgatcggcg ccgatctcgt cggccacgac gagcgcggac ttcgccaggg cgacgcctcg
     2041 gtcgcctcgg ccctggatga actcgaggcg tgcctgggtt gcgagcaccg agaccttggc
     2101 agtcgacgcc gggcggtctc ggatcagggc ggctgcctgt tcggcatgct cgtacgcctc
     2161 gtcccggcga gcttcgttcc acgccccgag cccgaggcct gcgtggacga gggcggcttc
     2221 ctcgacgcgt cccgctgcga cgagcggctc gacgagctcg gctccctccg tgagcggtac
     2281 ccgtgcggcc cacaacgcaa tcaggcgacg cgccgcggcg agcgtccgct gcggtgaccc
     2341 ctccggccag agctccgcgg cgcgtccgtg gaaacgcgcg gcggcctgga aggcgctcag
     2401 tgactccgcg cgctccgccg cgtcggtgag cgcctcccgg gctcgctcgt ccaggcctgc
     2461 cgtgtcgata cccgcctggc gcgcgtagga ggcggcctca ccccagtggt acgcaagcat
     2521 ttcggcgcgg tcctctaccc gctccgagag cgtctcgatc cactccgcgg tgcggctgtg
     2581 gaggacgctc ctcgtgcccc gcgggatctg gccatacgcg acgtcgcgga gcaacgcgtg
     2641 ccgaaatgcg tactcggtct caccgccgat cgacgaccgt cgttcgcgcc tgacgatgtc
     2701 cttgcgctcc agcccgtgca ggcggagctc gatgccctca gtcgcgcgac cgagtgcgac
     2761 gagcgcgccg cgccagaaga cctttcccac gacggccgct tcctggagca gcgccttttc
     2821 gtcaggtgct agcgcgtcca ggcgtgcggc gatgatgccc tgcacggtct cgggcagctc
     2881 cagggcggag ggatcgccgg cacggtcggc gagcatgcgc acgtactgct ctgcgtacag
     2941 cgggtttccg gccgcccggg cgagcaccgc ttctcgcacc tcgtcgggca gcgctgcccg
     3001 gtcggcgagc gccgccacca gctccgcggt ttcgccgtcc gagagcggcg acagggcgag
     3061 ggtcgtggca ttgagcttgc cgccgcccca gcccggccgc cgctcgagca gctcgggccg
     3121 ggcggtggcg agcacgaaga tcggcacgcc gcgggcccag tcggccagat ggtcgacgaa
     3181 gtcgagcagg ccgtcgtcgg cccaatgcag gtcctcgaag acgagcacca gcggccggac
     3241 gtcggccagg gcctcgaaga cctcccgcca ggcggcgaag ctctcggcgc tctctcctac
     3301 accttcgcgg acaaggccga cgagcggcct caggtgcggc tcgagacgat cggcgccctc
     3361 cggtgcgacg tcctcgagcg tctcccgcag cttgcgctct gcgtcggcgg aggagtcggt
     3421 gtcgagaacg ccggtctgcg ccttcatcat ctctgacagc gcccagaacg tcactccctc
     3481 gccgtacggc aggcagcggc cctgacgcca ccacacgagc tcgggcgcgg cgtcgacgac
     3541 gcggagcgtc tcgtacacga gccggctctt gccgatcccg gggacgccga cgagggtgac
     3601 gagctgggtc tcgcgctcgg actgcgagcg cccgagcgca ccgagcacca gctccagctc
     3661 gcgctttcgc ccgacgagag gtgccggcgc gccctgacgg acgtcgaggc cgagccgcgc
     3721 gcgaggctcg atcgcccgcc agacggcgac gggctccgtc ttgcccttcg cgcggatcgg
     3781 cgcggcggtc tcgtaggaga cgacgtgctc ggtcgcccgg taggtcgcct cgccgacgag
     3841 caccgagccg ggcggcgccg cggcctgaat gcgggcgccc gtgttcatca cgtcgcccga
     3901 gaccatgccc tcgcccgcct cggggcgcgc gtcgagggcg acgagcgcct cgcccgtgtt
     3961 gaccccgatt cgcacctcca ggtgcagctc aggatcctcc gcgttcaggt ccgcgatcgt
     4021 ctcctggatc ctgagcgccg cccgcactgc ccgctccggg tcgtcttcgt gcgcgaccgg
     4081 cgccccgaag accgccacga cggcgtcacc gatgaacttc tcaacggtgc cgccgaagcc
     4141 cgccagctgg tcgcggaggc tcgcatggaa cggcgcgagt acggcgcgta cgtcctcagg
     4201 gtcgagctcg tctgcgcgcg cggtcgagcc gaccatgtct gcgtagagga cggtcacgac
     4261 cttgcgctcc tcgcggggcg cgggcgcgtc ggcaagtcga gcgccgcaac ggccgcagaa
     4321 ggcaaagccg tcggggttgt cctggccgca ggtcgcgcac gtcggcacgc gcgagagcgt
     4381 actagcctag ccccgtgctt ccgcagcggc gaacggcgct ggtctccgta ggagccgccg
     4441 tcgccctgat ggcgatcaag ctcgtgacgg ggctcggcag cgggtcgctc ggtctcgtgg
     4501 ccgaagcgct gcactcgggt accgacctgg tcgctgctct gctcacgttc tacgccgtcg
     4561 gctatgccgt caagccggcc gacgtcgggc atccgtacgg acacggtaag gcggagcatc
     4621 tctccgccct cgcggaggcg gcggttctcg ccctcgtctc gatcggcgtg gccgctctcg
     4681 cggtcgggcg tctggcgggc tggctggaga ccgaggtcga tgcggcctgg tgggtgttcg
     4741 cggcgatcgc ggcggtgatc atgatcgacg tgtcgcgcac gatcgtctcg ctgcgcacgg
     4801 ccaggagatt caggagcgat gcgctgcttt cgaacgcggt ccacttcgcc ggcgacattg
     4861 ccggcacgct ggccgtgctc gccggcctcc tcgcggtgcg ggcgggctgg ccggagggag
     4921 actcgatcgc ggcgctgctc gtcgccgcgc tcgtgctcgt agcggcggcg aggctgatgc
     4981 ggcggaacgt caacgtgctg atggaccgcg cgccggcgga tgccgtcgcg gctgcgcggc
     5041 aggcgcttcg cgacgtcgag ccggcggtgg acgtgcgccg cctacggctt cgccaggcgg
     5101 ccgggcgcgt cttcgccgac gtcgtcatcc gcgtgccgcc tgacgaggcc gttgcccagg
     5161 ggcacgccgc cgccgacagt gttgaagagg cgctccacgt cgtgttgccg ggcagcgacg
     5221 tggtcgtcca cgtcgagccc gagggcggag atgcggttct ccgcgagcgc gtgcgcgccg
     5281 ccgcgatgac cgtgccgggc gtgcgggaga tccacaatct caccgtgata gatctcgacg
     5341 atggggtgac ggaggcctcg ctccacctca agctccccgg acagctctcg ctggacgagg
     5401 cgcacgatct cgcggaggcc gtcgagcagg ccatcgtcga tgctgtgccc gaggtctcgg
     5461 cggtgcaaac gcatctcgag cctctcgacg aggaggcggc tggacgcgag gtgacgaggg
     5521 ttcccgccga cgtcgccgag ctcgtcgcgg ctgccaccgg gcgggcgccg cgcgaagcgc
     5581 gcttgctgcg caccggcgac gggctcgttc tgttcctgac gctcgtcgtc gacgggggcg
     5641 cgacgcttgc cgaggcacac gaggaagcgg gagccgtcgc ggcacagatt cgggacgctc
     5701 accccgagat cgccgaggtg atcgtccaca ccgagccatg aaaggttgaa tacccaggtg
     5761 cggctctgca tgttccaccc actcgacagg ccgatggaga ggggctgggt cggccgcgtc
     5821 gacggcgaca gggtgattca cctcgcggcg cagaccctgc agtcgttctt catcggcggc
     5881 ggtggtgccc gggagcacgc ggattacgcg ctcgacggag tcgctttcct cgctccggtt
     5941 ctgcacccgc cgacggttcg tctcttcgac tcgcaggaca cgttctcgtt cgccaacgcg
     6001 acggcgatcg cgggccccgg cgccggcgtg aacggcggcc gcgcgcccgt ggtgctgccg
     6061 cgcatcgcgg cggtgatcga gggcgagcag cgtcccggcg gcttcaccgt gctggccgag
     6121 ttccgcgctc ccgaccgccc tgttccgaag gatcgcgact tcgcgctcgt tctcggtccg
     6181 gtcgtggtga cacccgacga gctcgagccg gatggcgtcg aggttgccgt gcggagcggc
     6241 gagacagagc gtctccgtgc cgtcgcacca cctttcgact ggcacgacgc gctgcgcctg
     6301 gccgcgttgg ggacgaagct gcgcccgggt gacatcgtcg tggggccggc ggcgggcgag
     6361 gtggcggtgg ggccgggcag catcgagctc ggtgtcgcca ggatcggcat cctcggcgcc
     6421 gaggtccggg acggctagcc ggcgacgcta gagattgccg cgcagcgcct gttcgcgctc
     6481 gatcgcctgg aagagagcct tgaagttgcc ttcgccgaag ccggtcgccc cgtgccgctc
     6541 gatgatctcg aagaagaggg tggggcgatc ctgtgcgttc ttcgtgaaga tctgcagcag
     6601 gtagccgtcc tcgtcgcggt cggcgaggat gccgagccgc tcgaggtccg cgtagtcctc
     6661 tgagatctcc ccgatgcgat cgggcaggtc ttcgtagtac gtgtgcggcg tgcggagcag
     6721 tgggagacca cgcttggcga gcccctcgac ggtgcgcacg atgtcggtcg tctgcattgc
     6781 gacgtgctgg gcgcccgggc cggagttgaa ctccaggtac tcctcgatct ggctcttctt
     6841 ctttccctcc gccggctcgt tgatcgggaa cttgatcttc cgctcgccgt ccgacatcac
     6901 cttggacatg agggccgagt actcggtctg gatctcgtcg ctgaagtgga tgatgttttc
     6961 gaagccgagc actcgcgcgt agtagtcgac ccactcgtcc atgcggccca gctcgacgtt
     7021 gccgacgacg tgatcgatcg cgaccaggcc gacgccgtcg tgcccgacgc cgtttcgcgc
     7081 cagcgagacg tacccgggca ggtacgggcc gccgtagtcg ctgcgctcga cgaaggtgtg
     7141 aacgacttcc ccgtaggttc cgattgccga gagtcgtacc cgaccgtaat cgtcgtcgag
     7201 cgtgtacggc tcggtgatgc cgcgcgcgcc gcgttggacg gcctgacggt aggcctcggc
     7261 gctgtccgag acgaggagcg ccacgtcctt cacggcgtcc ccgtggacgg cggcgaagcg
     7321 cgagatctcg ctgtcggcac gcagaccgct cgtgacgacg aagcggatct cgccctgctc
     7381 gagcacgtac gaggcgcggt cgcggacgcc cgtctcgggg ccggcgtacg cgacggggga
     7441 gaagccgagt gcgtgctcgt agaagaaagc cgcctgcttg gcgttcccaa cccagagctc
     7501 gacgtgatcc cagccgtcga ggggcatgaa gtcgctcatc tcggccctcc tcaggctctc
     7561 tgcgtgatcg gcgcagtcat cgtactggca gcctgcgtcg ggcgatccgt cgcctgccga
     7621 tgcggacgcg tctggctgag gcatcacatt cctctgaggt ccgttccgcg ctgcacgcgg
     7681 gttcgcgggc cccgattacc gtccgcggcg gccagtacgt gactgcccgc agcggacatg
     7741 tctcgccgag gggcgccctt ggcgggcaag gcggtaccgt gaatccatcg tctagcgtga
     7801 cctcatgcgg ctcgtgctcg actgggacgg cacggtcacc gaggtcgaca ccctccacct
     7861 cgtgatcgag cagttcggcg atctcggcgt cttccgagcg atggagggtg agctggagcg
     7921 gttgacgctg cacgacatca tcggcgcgga gatgcaaacg atcaccgcgc cgttggacga
     7981 ggtcgtcgca tggctactcg agaacgcgag gattcgcccg ggcttcgtcg agctcgtcga
     8041 cgcacatgag ccgaccatcg tgtccgcggg tttccacgag ctgatcgagc cgcttctcgc
     8101 gcaggcgggc gtcgaggttc ctgtgatcgc gaaccggatc gtctccgcgg cagcgtcggg
     8161 ctggcgtgtc gagttccgag acaccgccgt ctgtgagttc tgcggggagc cgtgcaagcg
     8221 ggccgccgcc gctgccgacg gtgccttcgc gtacgtgggc gacggcgtct ccgaccgatg
     8281 cgcgtcgctc gccgccgacc ggatcttcgc ccgggcgggc ctcgcccgtt acctcgacga
     8341 cgccggcgtc ccctacgagc cgttcgccga cctgcgcgac gtcgccgacg cactggccgc
     8401 cccgatctag ggacaccccc tcacaggatc gttacgagcc gctgacgaag agcgaacgcg
     8461 cggctcatct cggactgatg cctcgcgccg atcgttagct caacccccga ctccgacccc
     8521 aaggagggtt gagcaatgaa caagggaact ctcatcgccg cactcgttgt tctcggcgcg
     8581 cttctcgtgc cggtgaccgc agcggcggcg aagcacgagg tgagtgggcc gctcaccgtg
     8641 gccggcaagg gattcttccg cggcgagctc gagatggccg atcccggcga ttcgcgcccc
     8701 gtgaagatcg cgggcggccg tggctacgtg ggcgtcctgg acctcgcggg cgacctccgc
     8761 gtccggtgtc acggtcgcgg cgtcgcgaag aagaaggaga ccgacagggg caccgtgtac
     8821 ctctgcaagg gagcgcgcgg gatcgcggag ctcgtcggta gccacttccg cttccgcggc
     8881 ttcgcgaagc gcttcgtcat ccacctgccc gacggaacga ccggcaagct cgcaggccgc
     8941 ttgatccggc cgggcacggc gaacgatcgt ccgccccgcg gcgagcggcc cgacgctccg
     9001 gccgccgacg cacccgctga cggcgcggcg ggcgacgccg ccccgagcga cgagccggtc
//
LOCUS       DBNGNK010000189         3108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1679484,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000189 DBNGNK010000000
VERSION     DBNGNK010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1679484"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC150V_05505"
     CDS             <1..604
                     /locus_tag="AC150V_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3762413.1"
                     /translation="AALVGCGIATGVGAVLHCARVQPGSVVAVWGVGGVGLNVVAGAQ
                     IARAGTIAAIDPDPANLELAARRGASVLLEPEEAVEALADIGGVDVAFEAVGHEDAFL
                     AGLDSLAVGGHLVILGGVAPDSRVPVAPRTLVRKQARITGCIYGWIDPQRDFPVFAGW
                     CADGTIPVADLVSRTISLDELPALFTEKRPSGVRTVVNFD"
     gene            complement(610..1512)
                     /locus_tag="AC150V_05510"
     CDS             complement(610..1512)
                     /locus_tag="AC150V_05510"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017716110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3762414.1"
                     /translation="MPLDPQAAAWLEQVRALGVPAYHEVTPAEGRKLADEGAPALFGE
                     ADPVHSVEDAEADGVPVRIYRPSDAELPALVYFHGGGWVVGSLDSHDALCRTLAARSG
                     CVVVSVDYRLAPEHPFPAPVEDAWTATAWAEKTFPRIAIGGDSAGGHLAAVTALRARA
                     ASLALALQVLVYPVLDYSFDTASYVEHGSGTNLTEETMRWFWAQFVPDGAIADDPEVS
                     PLRARDLFGVAPALIVTAECDPLCDEAESYAQRLLAAGVPVALSRYDGLIHGFLRMPA
                     VLDRAEAGIDEVAEAVRIALGGKG"
     gene            1570..1770
                     /locus_tag="AC150V_05515"
     CDS             1570..1770
                     /locus_tag="AC150V_05515"
                     /inference="COORDINATES: protein motif:HMM:NF024851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR3762415.1"
                     /translation="MSVPRVEVEGDVCMGSASCVSLAPNTFRIGDDNVSLVIDPAGDP
                     LGDVLAAADACPTEAITVEPSE"
     gene            complement(2070..>3108)
                     /locus_tag="AC150V_05520"
     CDS             complement(2070..>3108)
                     /locus_tag="AC150V_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3762416.1"
                     /translation="AVVTYARKRLGSTDVELPELGLGGAPFGDLFAELGDDVVDATMR
                     AAWGSGIRFFDTAPHYGAGLSEHRMGRFLRAVPRDEFLLSTKVGRVLRPAPGDGSLER
                     GPFVGGLPFEQVYDYGYDGVRRSHEDSLQRLGLARVDLLLIHDLDVAHHPDPDVRARH
                     LDDVAGGGARALDELKSEGAISAVGAGVNEPGMIRVLLERVALDFVLLARPYTLLDQH
                     ALDEGLPLCEERGVGVVIGSVFNSGILAAERAAEAPYNYAVAPGDVIDRVERIRVVCD
                     RHDVRLPAAALRFPLGHPSVAAVIPGAVEPAQVETNAGHIRQEIPIALWADLKAEGLL
                     RAEAPVPAASG"
BASE COUNT          472 a         1098 c         1089 g          449 t
ORIGIN      
        1 ggccgcgctc gtcggctgcg gcatcgcaac gggcgtgggc gccgtactgc actgcgcgcg
       61 ggtgcaaccc gggtcggtcg tcgcggtttg gggggtaggt ggggtgggcc tcaacgtggt
      121 ggcaggcgca cagatcgccc gggccggcac gatcgccgcg atcgaccccg acccggccaa
      181 cctcgagctc gcagccaggc ggggagcgag cgtgctgctc gagcccgagg aggcggtaga
      241 ggcgctggcg gacatcggcg gcgtcgacgt cgccttcgag gccgtagggc acgaggacgc
      301 gtttctcgcc ggactcgact ccctggcggt gggaggccat ctcgtgatcc tcggcggcgt
      361 cgccccggac agtcgcgtcc cggtggcgcc acgaaccctt gtgcggaagc aggcacggat
      421 cacgggctgc atctacggct ggatcgaccc gcagcgggat ttcccggtct tcgccggctg
      481 gtgcgcggac ggcaccattc cggtcgccga tctcgtctcg cgcacgatct cgctcgacga
      541 gttgcccgct ctgttcacag agaagcggcc gtccggcgtg cgaacggtcg tcaacttcga
      601 ctagctgatc tagcccttgc cgcccagggc gatccgaacc gcctcggcca cctcgtcgat
      661 gccggcctct gcccggtcga gcacggccgg catgcggagg aagccgtgta tcagcccgtc
      721 gtaccggctc aaggcgacgg ggacccccgc tgcgaggagc cgctgtgcgt acgactcggc
      781 ctcgtcgcag agtgggtcgc actccgccgt gacgatcagt gccggcgcca cgccgaagag
      841 atcccgcgcc cgcagcggtg acacctctgg atcgtcggcg atcgctccgt cgggaacgaa
      901 ctgcgcccag aaccagcgca tcgtctcctc tgtcaagttc gttccgcttc cgtgctccac
      961 atacgaagcg gtgtcgaacg agtagtcgag gaccggatac acgagaacct ggagcgcgag
     1021 cgccaggctc gccgcccggg cgcggagtgc ggtcaccgcc gcgagatgcc cgcccgcact
     1081 gtcacccccg atcgcgatcc tcggaaaggt tttctccgcc cacgccgtcg cagtccacgc
     1141 atcctcgacc ggcgcaggga acgggtgctc gggcgccagc cggtagtcga ccgagacgac
     1201 cacgcatccc gaccgggccg ccagggtgcg acagagtgcg tcatgggagt ccaggctgcc
     1261 gaccacccat cccccaccgt ggaagtacac gagcgccggc aactcggcgt cgctgggacg
     1321 gtagatgcgg acgggcacgc cgtcggcctc ggcgtcctcg acggagtgca ccgggtcggc
     1381 ctcgccgaag agcgccgggg caccttcgtc ggcgagcttc ctgccttcgg cgggtgtcac
     1441 ctcgtggtag gccgggacgc cgagcgcccg gacctgttcg agccacgccg ccgcctgcgg
     1501 gtcgagaggc acggctggac attaaccgct cggagaacca ccgtgctcga atcgcgtacg
     1561 ctacgcgcga tgtccgtgcc gagagtggaa gtcgagggcg acgtctgcat gggctcggcg
     1621 tcgtgcgtgt cgctggcgcc gaacacgttc cggatcgggg acgacaacgt ctcgctggtc
     1681 atcgacccgg caggcgaccc gctcggtgac gtcctcgccg ccgccgacgc ctgccctacc
     1741 gaggcgatca ccgtcgagcc gagcgagtag ggcgatcgac ccctgacaca ggcgaaaggg
     1801 gtgcgcgagt accgggacgc gcgcactcgg cgaatcgccg tcgcgccttg cggcagctgt
     1861 acgcgtttcc gacaggcgcg tccggtcggg acgatcatgt agtggctgcc ggggacgggt
     1921 tcgaggtcgc tgaaccctgt ttgccgcctc aactggacct cagaggaatg tgatgccgcc
     1981 tccggacacg tctggagcgc cggcgcagcg taccccttca gacgctcgat cccgagtcgc
     2041 ctgacgggcc tgcttcttgg cgactgcgct cagccgctcg cagctggaac gggagcctcc
     2101 gcacggagca gtccctcggc cttgaggtcg gcccagagcg caatcgggat ctcctgccgg
     2161 atgtgccccg cgttcgtctc gacttgcgca ggctcgacag cgccgggaat gacggcggcg
     2221 accgacgggt ggccgagagg gaagcggagc gcggcagccg gcagccgcac gtcgtggcgg
     2281 tcacagacca cccggatccg ctcgacacga tcgatcacgt cacccggcgc gaccgcatag
     2341 ttgtaaggag cctcggcagc tcgctccgcc gccaggatcc cggagttgaa aacggagccg
     2401 atgacgacgc cgactcccct ctcctcgcac agcggcaggc cctcgtcgag ggcgtgctgg
     2461 tcgagcagcg tgtacggccg cgcgagcagg acgaagtcga gcgcgacccg ctcgagcagc
     2521 acgcggatca tcccgggctc gttcacgccg gctccgaccg cgctgatcgc gccctcgctc
     2581 ttcagctcgt cgagcgccct cgcacctccg ccggccacgt cgtcgaggtg tcgggcccgg
     2641 acgtctgggt ccggatggtg ggcgacgtcg aggtcgtgga tcagcaacag atcgacccgc
     2701 gccagcccga ggcgttggag cgagtcctcg tgtgagcgcc ggacgccgtc gtacccgtag
     2761 tcgtagacct gctcgaacgg cagcccgccg acgaaggggc cccgctcgag cgagccgtcg
     2821 cccggagcgg gccgcagcac acggcccacc ttggtcgaga gcaggaactc gtcccgcggc
     2881 acggctctga ggaagcggcc catgcggtgc tcgctcaagc ccgcgccgta gtgaggagcc
     2941 gtgtcgaaga agcgaatccc actgccccag gccgcgcgca tcgtggcgtc cacgacgtcg
     3001 tctccgagct cggcgaagag gtcgccgaac ggcgctccgc cgagcccgag ctcgggcagc
     3061 tcgacgtcgg tgctgccgag gcgcttccga gcgtatgtca cgactgcg
//
LOCUS       DBNGNK010000190        10434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1465800,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000190 DBNGNK010000000
VERSION     DBNGNK010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10434
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1465800"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="AC150V_05525"
     CDS             complement(<1..882)
                     /locus_tag="AC150V_05525"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3762417.1"
                     /translation="MAVGTRTLENFIGGAWVPSSGDRFVDDVNPADPGDVLARVPLSP
                     AADAVAAIDAAEAAFAEWRATSPVRRGQILARTSRVIESRQEEIAQLITREQGKTLAE
                     ARGETARVIQFMAWIGQQGTAITGVTAPTEADRMLGVTLREPLGVVALITPWNFPLNI
                     PSWKLGSSLLCGNTVVLKPAAPTPLCAIALVEALAEAGLPDGVANLVLGSGSEVGGTL
                     VTDPRIKAISFTGSTAVGRSINEQAAPLGKKVQAEMGGHNAVIVLGDADLERAAVGCA
                     AAGYGTTGQRCTAPRRII"
     gene            complement(905..1681)
                     /locus_tag="AC150V_05530"
     CDS             complement(905..1681)
                     /locus_tag="AC150V_05530"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762418.1"
                     /translation="MADLQDRRVVITGASQGIGAAAAIGFAAAGAHVAFDFRSAVDEA
                     EKTAAAVREHDREAIVLQGDMGDPAHVDELAAAAADAWGGIDVWVNNAARLLVKPFLE
                     MTDEDWHGLHAANLHGYYYGCRAAARQMEKQGGGRIINVTSVVDIQPIAELSAYVTAK
                     GAIIGLTKTLAVELGPLGITVNALSPGATDTPLNQTAYTPEVRRNYEERIPLGRIGSP
                     EEVADAIVALAGDAFRYTTGVELLVDGGLVLNGNVGHART"
     gene            complement(1683..2876)
                     /locus_tag="AC150V_05535"
     CDS             complement(1683..2876)
                     /locus_tag="AC150V_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3762419.1"
                     /translation="MADEPEVGFVTMGQAASTEDIPSIGIGMLGYSFMGKAHTNALKK
                     ISYITYPPPYFPRLVSIAGRTEDAVKEAAQRYGYEKWTTDWNDVVNDPDVQVFDNGGP
                     NDTHLEPTIAAAKAGKHVILEKPLGRTADESYEIWKGVEGTGVKTQCAFNYRFFPAIR
                     LAKEMIDAGEIGEIYHFRGRYHQEWIMDPEFPKVWRLDKSVAGSGALGDLGAHVIDQS
                     RYLCGEPSAVTGVLRTFIKERPGGTVDVDDAFEATVEFENGAIGTYEATRFALGRKNQ
                     MRWEINGSKGTIIFDQERMNELVVNFGNSKPGEHAQGFRTVLVSEAYHPYWEYHWPHG
                     HMIGWEDSFLHELLHLLTCIREDKPIGPHGATFEDGYRCAEICDAIVRSHETGSRVPI
                     EYRSL"
     gene            complement(2921..3901)
                     /locus_tag="AC150V_05540"
     CDS             complement(2921..3901)
                     /locus_tag="AC150V_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018760674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3762420.1"
                     /translation="MKIGLFTVMYNDKPLEDVAKHASGLGYEMFELAAWRGSNHFDPD
                     KAAEDKNYGKNIKKMLDGHGIEISALSNHLFSQMVLPFQDSSLDEWAGTSDKEEMWKL
                     GREQIIATAQYASDLEIKTVVGFFGSTVWESWYIWPPQRLDIYEQGWELFVERWNPIL
                     DEFKKLGVTFAHEVHPTEIAYNVYTAREAIKRLDRDDWGFNFDPSHMVWQMIDPVVFI
                     KEFGDRIYHAHAKDWELQKDILHIDGVTSTGSWQRGDRAARYRVPGWGDVEWRRVITA
                     LLEVGYDFVLSFEHEDPVMSEDDGLEQAIKYLEPLIIKKPLVPGQAWWLA"
     gene            complement(3905..4930)
                     /locus_tag="AC150V_05545"
     CDS             complement(3905..4930)
                     /locus_tag="AC150V_05545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020111340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3762421.1"
                     /translation="MGLTKLASGPGNVALAERPERRPGAGEVLLEVRGAGVCGTDLHI
                     EADEFRSEPPVTMGHEVSGLVVELGEGVDAGWAGARVVSETYFSTCETCEWCRAGRQN
                     LCPERRSIGSFVDGAFAPRLVVPARNLHRIPDWLDEHAAALAEPLACVCHCLCDAPLV
                     SAGDSVLVTGPGPVGLLAAQVARAHGGVVTVVGLPSDEVRLATATELGFTTAHVADPD
                     WADVVIECSGAEGGARACLDAAGRGARYVQIGVFGKEVTLPIDTTFLKELVITSGFAS
                     TPGSWRRALALIERRSVELEPLVSEVVPLRAWERVFSELRAGKGIKIVFDPRLDETQS
                     TPPTPGG"
     gene            complement(4930..6141)
                     /locus_tag="AC150V_05550"
     CDS             complement(4930..6141)
                     /locus_tag="AC150V_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3762422.1"
                     /translation="MKITGVKTHIVDTVLPEEHRVRSGAGYKLRRQAAFVELTTDEGI
                     SGHGPCSFGSASLDLGSVASLCDNTFAAALVGEDPHRIEYLWDKIYYGSILRVHGARS
                     VGVAILSAIDIALWDIKGKALGAPVYQLLGGAFQDPVPCYSSSVYWDTPEGAAQQARA
                     FVDQGFKAFKVKVGLDVENDIASLHAIRDEVGYEVDMLVDANQCYTRHLALRVGRELD
                     KLNVPFYEEPLSIDDVDGHAFLAERLDVRIATGENMYTRWDFLPFFQKQAVHVVQADA
                     SRCGGISEAKRIADLAGSYHLHAIPHTFSDALTTAANLHVVAASSNAPIIEYDRTYNE
                     IQEKLVKNPPLVHDSVIELPVDPGLGVEIDWDFVAAHPFSGEIGIGAGSRPAFGLATE
                     VIRDRTMTSLA"
     gene            complement(6138..7304)
                     /locus_tag="AC150V_05555"
     CDS             complement(6138..7304)
                     /locus_tag="AC150V_05555"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR3762423.1"
                     /translation="MPWDSVHRFHCPTRLFYGRGASAEAGERLAELGASRVLLISDPG
                     LEAAGAVGTLVERIESAGLDVHVYAKTEPNPTTTNVHEAAVLYREAGCDGIVGLGGGS
                     SMDCAKGAGIIVSNGGEIGDYRGPDKVPTRVPPLVCIPTTCGTGSEVTTVAVITDPNV
                     DFKLVIVSPNIGPDIGLVDPDLVQTAPSQVIAATGIDALAHAVESYVNTGSDPLLDSL
                     NIGAIRMIGANLRRAVADGDPDAVAQVTLASMIAGVAFNMNGNAIVHAASTPVTARHH
                     VPHGVANGIFLPAGLEAISAGCRAKLGEIAAALGEDVSGLPEEDAAAAGVRAIRSLCA
                     DIGIPDTLRQWGIDPAQVDIPQLVEDAMKSRNIATNPVPVGPEELATLYTVVVG"
     gene            complement(7311..8042)
                     /locus_tag="AC150V_05560"
     CDS             complement(7311..8042)
                     /locus_tag="AC150V_05560"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3762424.1"
                     /translation="MTVAPRKRETLPATIAGSLRRKISTGELAPGDQLPGHRELAQQH
                     GVSLSSVREAISMLVSGGYVETRAGRGTYVSLEPPPAARVGPPLSRAEVEELVEARAV
                     IEVQLAQFAAQRATPEQSKALLAAVERMEAASSSTTSYPDADVDFHIALAAAANNRYL
                     LQAMNDIGALLRDDMELGAEAAIRRFGDLSISVDSHRRLAEAVAAHDPETAKAIAAEI
                     INRNRAFVLGLYALGSEADRPAPAT"
     gene            8446..10170
                     /locus_tag="AC150V_05565"
     CDS             8446..10170
                     /locus_tag="AC150V_05565"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3762425.1"
                     /translation="MEEEQVLEESNDQPEGEVEEQATSGKMSRGKFMAAAGAAGAAVA
                     AGSLPTVAGAKPYTASKRNRALQSKLAPGKIGGPVGFKGASRYQYGPNDEEGRAMRAL
                     QALKKAGNAPDQLVVQVLNFARPQFETKFPKGATETISQLFERESGIKLKWVETDPAT
                     EFQTNIRNASTKNGSFDLVTGAIEDTGDYASAGLLLPLDKFVNKYKPSWNSAKDGYAG
                     GRATVNLFTKFEGTTYWVAFDNDTQPYVYRNDLWNSPKEKAAFADKYGYPLAPPKTWD
                     QQADMAAFFHRPKAQNPLFGSVERKGPFWGKVNWQHRFVSSGDPNQYYFKPDGSANVN
                     NTAGIRAATEHLRSLQWSEPGNLSKDWLAQYQLFGGGNGVQGGTFPNVTKLVVPANKD
                     LDKGFGKFLRTDVQPGRIVNGKLVRRVAIHYNISYGVNAFAPKANHEAAYLFLQWAGG
                     RRMYTYLTASPGGYQDPHHTASLTDPLVVQSYGAQPMRAFAQIVPRQVPPITIRGAAE
                     YNQALDEELQKMLTKQQSPERAMRNVANRWDKITDRLGSGRQAKAVKQSYVAFPPKGR
                     YGPNKKIT"
     gene            10192..>10434
                     /locus_tag="AC150V_05570"
     CDS             10192..>10434
                     /locus_tag="AC150V_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762426.1"
                     /translation="MTETTEQHPTRQASKGRLSDVGGRNESAVSSLLILPGRLMLLFI
                     VLFPAGVAIYLGFTEWTPTSGTDVWGAYKFWHWFDGY"
BASE COUNT         1650 a         3508 c         3596 g         1680 t
ORIGIN      
        1 gatgatccgg cgaggggccg tgcagcgctg cccggtggtg ccatagccgg cggcggcaca
       61 tcccacggcc gctcgctcga ggtcggcatc cccgagcacg atcaccgcgt tgtggccgcc
      121 catctccgcc tgcaccttct tgccgagcgg ggccgcctgc tcgttgatgc tgcgccccac
      181 ggcagtcgag ccggtgaacg agatcgcctt gatgcgcggg tcggtcacga gcgtgccacc
      241 cacctccgag ccggagccga gcacgaggtt cgcgacgccg tccgggaggc cggcctcggc
      301 gagcgcctcg accagcgcga ttgcgcagag cggtgtcggc gctgcgggct tgagcacgac
      361 ggtgttgccg cacaagagcg acgagccgag cttccagctc gggatgttga gcgggaagtt
      421 ccacggcgtg atcagagcga cgacgccgag gggctcgcgg agcgtcacgc cgagcatccg
      481 gtcggcctcg gtcggcgccg tgacacccgt gatcgcggtg ccctgctgcc cgatccacgc
      541 catgaactgg atcacccgcg ccgtctcgcc acgggcctcg gcgagcgtct ttccctgctc
      601 gcgcgtgatc agctgggcga tctcctcctg ccgcgactcg atcacgcggg aggtgcgggc
      661 gaggatctgg ccgcgccgga ccggagacgt cgcccgccac tcggcgaagg ccgcctcggc
      721 agcatcgatc gcggccactg cgtccgcggc cggcgagagc ggcacgcggg cgagcacgtc
      781 gccggggtcg gccgggttga cgtcgtcgac gaagcggtca ccgctcgacg gcacccaggc
      841 gccgccgatg aagttctcga gcgtccgtgt tcctaccgcc actgcgaatc ctttcctctg
      901 ttcctcacgt gcgggcatgg ccgacgttgc cgttcagcac gaggccgccg tcgacgagca
      961 gctccacgcc ggtcgtgtaa cggaacgcgt cgccggccaa ggcgacgatg gcatccgcga
     1021 cctcctccgg cgagccgatg cgcccgagcg ggatgcgctc ctcgtagttg cggcgcacct
     1081 ccggcgtgta cgccgtctgg ttgagcggcg tgtcggtggc acccggcgag agggcattga
     1141 ccgtgatccc gagcgggccg agctccacgg cgagcgtctt cgtcagtccg atgatcgcgc
     1201 ctttcgcggt tacgtaggcg gagagctctg cgatcggctg gatgtcgacg accgaggtca
     1261 cgttgatgat ccgtccgccg ccctgctttt ccatctgccg cgcggcggcc cggcagccgt
     1321 agtagtagcc atgcaggttg gcggcgtgga gcccgtgcca gtcctcgtcc gtcatctcga
     1381 ggaacggctt gaccagcagg cgggcggcgt tgttcaccca gacgtcgatg ccgccccagg
     1441 cgtcggcggc agccgcggcg agctcgtcca cgtgagccgg gtcgcccatg tcgccctgca
     1501 ggacgatcgc ctcacggtcg tgctctcgca ccgccgccgc ggtcttctcc gcctcgtcga
     1561 ccgccgaccg gaagtcgaaa gcgacgtggg ccccggcggc ggcgaacccg atcgccgccg
     1621 ccgcgccgat gccctgcgac gcccccgtga tgacgacgcg ccggtcctgc aggtccgcca
     1681 cgctagaggc tcctgtactc gatcggcacc cgggagccgg tctcgtgcga gcggacgatc
     1741 gcgtcgcaga tctccgcgca gcgatagccg tcctcgaagg tcgctccgtg agggccgatc
     1801 ggcttgtcct cgcgaatgca ggtgaggaga tggagcagct cgtggaggaa gctgtcctcc
     1861 cacccgatca tgtggccgtg cggccagtgg tactcccagt acgggtggta ggcctccgag
     1921 acgagcaccg tgcggaagcc ctgagcgtgc tcgccgggct tcgagttgcc gaagttcacc
     1981 acgagctcgt tcatccgctc ctggtcgaag atgatcgttc ccttcgaccc gttgatctcc
     2041 cagcgcatct ggttcttgcg gccgagcgcg aaccgcgtcg cctcgtaggt gccgatggcg
     2101 ccgttctcga actcgacggt ggcctcgaac gcgtcgtcga cgtcgaccgt cccgccgggg
     2161 cgctccttga tgaacgtccg gagcacaccg gtgactgcgg acggctcgcc gcagaggtag
     2221 cgcgactggt cgatcacgtg cgcgccgagg tcgccgagcg ctcccgagcc ggcgacggac
     2281 ttgtcgagcc gccagacctt ggggaactcg ggatccatga tccattcctg gtgataccgg
     2341 ccccggaagt ggtagatctc gccgatctcg ccggcgtcga tcatctcctt ggccaggcgg
     2401 atcgcaggaa agaagcggta gttgaacgcg cactgcgtct tgacgcctgt gccctcgacg
     2461 cccttccaga tctcgtagct ctcgtcggcc gtgcggccga gcggcttctc caggatgacg
     2521 tgcttgccgg ccttcgccgc ggcgatcgtc ggctcgaggt gcgtgtcgtt cgggccgccg
     2581 ttgtcgaaga cctggacgtc cggatcgttg acgacgtcgt tccagtcggt cgtccacttc
     2641 tcgtagccgt accgctgcgc cgcctccttc accgcatctt cggtgcgacc cgcaatcgag
     2701 acgaggcgcg ggaagtacgg gggcgggtac gtgatgtagg agatcttctt gagagcgttc
     2761 gtgtgggcct tgcccatgaa cgagtagccg agcatgccga tgccgatcga ggggatgtcc
     2821 tcggtcgagg cagcctggcc catcgtcacg aagccgacct ctggttcgtc tgccatcgct
     2881 cagtcctttc tcgggctcga gggggtcggg cgcgcgcagg tcaggcgagc caccacgctt
     2941 ggccgggcac gagcggtttc ttgatgatca gcggctcgag gtacttgatc gcctgctcga
     3001 ggccgtcgtc ctcggacatg accgggtcct cgtgctcgaa ggagagtacg aagtcgtagc
     3061 ccacctccag cagcgcggtg atcacgcggc gccactcgac gtcgccccag cccgggacgc
     3121 ggtagcgcgc tgcacggtca ccccgctgcc aggagccggt gctcgtgaca ccgtcgatgt
     3181 ggaggatgtc cttctggagc tcccagtcct tggcgtgggc gtggtagatc cggtcgccga
     3241 actccttgat gaagacgacg gggtcgatca tctgccagac catgtggctg gggtcgaagt
     3301 tgaagcccca gtcgtcccgg tcgagacgct tgatcgcctc gcgggccgtg tagacgttgt
     3361 aggcgatctc tgtcgggtga acctcgtgtg caaaggtgac gccgagcttc ttgaactcgt
     3421 cgaggatggg gttccagcgc tccacgaaga gctcccagcc ctgctcgtag atgtcgaggc
     3481 gctgcggcgg ccagatgtac cagctctccc agaccgtcga gccgaagaag ccgaccacgg
     3541 tcttgatctc gaggtcgctc gcgtactgcg ccgtcgcgat gatctgctca cggccgagct
     3601 tccacatctc ctccttgtcg gaggtgccgg cccactcgtc gagggacgag tcctggaacg
     3661 ggagcaccat ctgcgagaaa aggtggttgg agagcgccga gatctcgatc ccgtgcccgt
     3721 cgagcatctt cttgatgttc ttgccgtagt tcttgtcctc ggccgccttg tcggggtcga
     3781 agtggttcga gccgcgccag gcggcgagct cgaacatctc gtagccgagg cccgaggcgt
     3841 gcttcgcaac gtcctcgagc ggcttgtcgt tgtacatgac ggtgaagagt ccgatcttca
     3901 tgactcagcc tcctggggtc ggtggggtcg actgggtttc gtccagacgt gggtcgaaga
     3961 cgatcttgat cccttttccg gcgcggagct cggagaacac gcgctcccat gcacgaagcg
     4021 ggacgacttc gctgacgagc ggctcgagct cgacgctccg ccgctcgatc agcgcgagcg
     4081 cgcgtcgcca cgagccgggg gtcgaggcga agcccgaggt gatcacgagc tccttcagga
     4141 aggtcgtgtc gatggggagc gtgacctcct ttccgaagac gccgatctgc acgtagcggg
     4201 cgccgcgacc ggcggcgtcg agacacgctc gggcgccgcc ttcggcgccc gagcactcga
     4261 tcacgacgtc ggcccagtcc ggatcggcga cgtgcgcagt cgtgaagccg agctcggtcg
     4321 ccgtggcgag gcgcacctcg tcgctcggta ggccgacgac cgtgacgacg ccgccgtgcg
     4381 cgcgcgcgac ctgcgccgcg agcaggccga ccgggccggg accggtcacg agaacgctgt
     4441 cgcccgcgct cacgagcggc gcgtcgcaca ggcagtggca cacgcacgcg agcggctcgg
     4501 cgagcgccgc cgcatgctcg tcgagccagt ccgggatccg gtgcaggttt cgcgcgggga
     4561 cgacgagccg cggcgcgaag gcgccgtcga cgaaggagcc gatcgagcgg cgctcgggac
     4621 agaggttctg ccgacccgcg cgacaccact cgcacgtctc gcaggtcgag aaatacgtct
     4681 cggagacgac gcgggcgccg gcccaaccgg catcgactcc ctcaccgagc tcgacgacga
     4741 gcccgctgac ctcgtgtccc atggtcaccg gaggctcgct cctgaactcg tcggcctcga
     4801 tgtgcaggtc ggttccgcag acgcccgcgc cgcgaacttc gagcagcacc tcgccggcgc
     4861 cgggccggcg ctcgggtcgc tcggccaggg cgacattgcc cgggccggaa gcgagcttgg
     4921 tcagccccat cacgccagag acgtcattgt ccgatcgcgg atcacctcgg ttgcgagccc
     4981 gaacgcgggc cgggagccgg cgccgatccc gatctccccc gagaacggat gcgcggcgac
     5041 gaagtcccag tcgatctcga cgccgagccc ggggtcgacc ggaagctcga tcacgctgtc
     5101 gtggacgagc ggcgggttct tgacgagctt ctcctggatc tcgttgtacg tgcggtcgta
     5161 ctcgatgatc ggcgcgttcg acgacgcggc gacgacgtgg aggttcgcgg ccgtggtgag
     5221 cgcatccgag aacgtgtggg gaatcgcgtg gaggtggtac gaaccggcca gatcggcgat
     5281 gcgcttcgcc tcgctgatgc cgccgcagcg cgaggcatcg gcctggacga cgtgcaccgc
     5341 ctgcttctgg aagaagggca ggaagtccca gcgggtgtac atgttctcgc cggtcgcgat
     5401 ccggacgtcg agccgctcgg cgaggaaggc gtgcccgtcg acgtcgtcga tggagagcgg
     5461 ctcctcgtag aagggcacgt tcagcttgtc gagctcacgg cccacccgca gggcgaggtg
     5521 gcgcgtgtag cactggttgg cgtcgacgag catgtcgacc tcgtagccga cctcgtcccg
     5581 gatcgcgtgc agcgacgcga tgtcgttctc gacgtccaac ccgaccttga ccttgaaggc
     5641 cttgaagccc tggtcgacga acgcccgcgc ctgctgcgcc gcgccttccg gcgtgtccca
     5701 gtagacggag ctcgagtagc agggaaccgg atcctgaaac gcgcctccga gcagctggta
     5761 gacgggcgcg cccagtgcct tgcccttgat gtcccagagc gcgatgtcga ttgccgagag
     5821 gatggccacg ccgaccgagc gcgcgccgtg cacacgcagg atcgagccgt agtagatctt
     5881 gtcccagagg tactcgatcc ggtgcgggtc ctcgccgacg agcgcggcgg cgaacgtgtt
     5941 gtcacagagg ctcgcgacgg agccgaggtc gagcgacgca ctcccgaacg agcagggtcc
     6001 gtgaccggag atcccctcgt cggtcgtcag ctcgacgaac gcggcctgcc ggcgcagctt
     6061 gtagccggcg ccgctgcgga cgcggtgctc ctcggggagg acggtgtcga cgatgtgggt
     6121 cttgacgccg gtgatcttca tccgacgacc acggtgtaca gggttgccag ctcttcgggg
     6181 cccaccggga ccgggttggt ggcgatgttg cggctcttca tggcgtcctc cacgagttga
     6241 ggtatgtcca cctgcgcggg atcgatgccc cattggcgca gggtgtcagg gatgccgatg
     6301 tctgcacaga gggagcggat cgcccgcacg cccgcggcgg cggcatcctc ctcggggagg
     6361 ccggagacgt cctcgccgag ggcggcggcg atctcaccga gcttggcccg gcagccggcc
     6421 gagattgcct cgagcccggc cggcaggaag atcccgttcg cgacgccgtg cggcacgtga
     6481 tgccgcgcgg tcaccggcgt cgacgccgca tggacgatgg cgttcccatt catgttgaac
     6541 gccactccgg cgatcatgct cgcgagcgtc acctgcgcga cggcatccgg gtcgccgtcg
     6601 gcgacggcac ggcgcaggtt ggcgccgatc atgcggatcg caccgatgtt gagcgagtcg
     6661 agcagcgggt cggagccggt gttcacgtag gactcgaccg catgcgcgag agcgtcgatt
     6721 cccgtcgcgg cgatgacctg cgacggcgcc gtctgcacga ggtcggggtc gacgagaccg
     6781 atgtcggggc cgatgttggg cgacacgatc acgagcttga agtcgacgtt cggatcggtg
     6841 atcaccgcga ccgtggtcac ctccgagccc gtgccgcagg tcgtcgggat gcagacgagc
     6901 ggcggcaccc gtgtgggcac cttgtccggg ccgcggtagt cgccgatctc gccgccgttg
     6961 gagacgatga tgcccgcacc cttggcgcag tccatcgagc tgccgccgcc gaggccgacg
     7021 atcccgtcac agcccgcctc ccggtacagc accgcggcct cgtgcacgtt cgtggtcgtc
     7081 ggattcggct cggtcttcgc gtacacgtgc acgtcgagac ccgcggactc gatgcgctct
     7141 accagcgtcc cgaccgcccc tgcggcttcg aggccggggt cggagatcag gagcacgcgg
     7201 gaggcgccga gctcggccag ccgctcgccg gcctcagccg atgcgccgcg accgtagaag
     7261 agccgtgtcg ggcagtgaaa ccgatgaacc gagtcccagg gcacttgtcg ctacgtggcg
     7321 ggtgcaggcc ggtcggcctc cgaccccagc gcgtagaggc caagcacgaa ggcgcggttg
     7381 cggttgatga tctccgccgc gatcgccttc gccgtctcgg gatcgtgcgc ggcgaccgcc
     7441 tcggccaggc ggcggtgcga gtcgacgctg atgctgaggt cgccgaaccg gcgaatcgcg
     7501 gcttcggcgc ccagctccat gtcgtcgcgg agcagtgctc cgatgtcgtt catggcctgg
     7561 agcaggtatc gattgttcgc cgccgcggcc agcgcgatgt ggaagtcgac gtcggcatcc
     7621 gggtaggagg tcgtgctcga ggacgccgct tccatcctct cgaccgccgc cagcaacgcc
     7681 ttgctctgct ccggcgtcgc gcgttgcgcg gcgaactgag cgagctgcac ctcgatcacg
     7741 gcacgagcct cgacgagctc ctccacctcg gcgcgagaca gaggcggccc gacacgggcc
     7801 gctggcggcg gctcgaggct gacgtaggta ccgcgacccg cgcgtgtctc gacgtagccg
     7861 ccgctgacga gcatgctgat cgcctcgcgg acggagctca agctgacccc gtgctgctgt
     7921 gccagctccc ggtgcccggg gagctgatcg cccggcgcga gctcgccggt cgagatcttg
     7981 cgccgcagcg agccggcgat ggtggccggc agggtttcac gtttcctcgg cgccacggtc
     8041 atgactgttt cactgttcag aacggttctg gcgcaggcga agctaccagc cgtcggggtc
     8101 tctggtcaac tagcgcgata cgagctggcg cgtgtaaacg attaccggtt cttcctatag
     8161 gcgtatccct ctggacaagg aagggcaggc ttgatatagg tggcccccat cgctgcactg
     8221 ttccgatcag ttccgctgtt tccgcactcg gccagactgg gagggtctgt cggcaccggt
     8281 gcgtcaaact tccgaccggc ggcggagtct caacacgtgc aaccgttcag cggaccggag
     8341 cggcgagaaa gggcacccaa ggggtctagt ccagtagcgt cggccagggc ttagcagggg
     8401 ccgaaccgcg ccggcctgtc cttacggcat aaggaggaag cagtcgtgga ggaggagcaa
     8461 gtgctagagg agagcaacga tcagccggag ggtgaggtcg aagagcaggc cacctcgggc
     8521 aagatgtcgc gcgggaagtt catggccgcg gccggcgccg cgggtgcggc ggtcgccgca
     8581 ggcagccttc ccacagtcgc aggcgcgaag ccgtacacgg cgtccaagcg caaccgggcg
     8641 ctgcagagca agctcgcgcc cggcaagatc ggcggcccgg tcggcttcaa gggcgcctcg
     8701 cggtaccagt acgggccgaa cgacgaagag ggacgcgcca tgcgggcgct gcaggcgttg
     8761 aagaaggcgg ggaacgcccc cgaccagctc gtcgtccagg tgctcaactt cgcccggccg
     8821 cagttcgaga cgaagttccc gaagggcgcg accgagacca tctcccagct cttcgagcgg
     8881 gagagcggaa tcaagctgaa gtgggtcgag accgacccgg ccaccgagtt ccagaccaac
     8941 atccggaacg cctcgacgaa gaacggcagc ttcgatctcg tcacgggcgc gatcgaggac
     9001 accggcgact acgcgagcgc cggcctcctg ctcccgctcg acaagttcgt caacaagtac
     9061 aagccgagct ggaactctgc gaaggacggc tacgcgggcg gccgcgcgac cgtcaacctg
     9121 ttcacgaagt tcgagggcac gacctactgg gtcgcgttcg acaacgacac gcagccgtac
     9181 gtctaccgga acgacctctg gaacagcccg aaggagaagg cggcgttcgc cgacaagtac
     9241 ggctatccgc tcgcaccgcc caagacctgg gatcagcagg cggacatggc ggcgttcttc
     9301 caccgcccga aggcccagaa cccgctcttc ggctccgtcg agcgcaaggg cccgttctgg
     9361 ggcaaggtca actggcagca ccgcttcgtc agctcgggcg atccgaacca gtactacttc
     9421 aagcccgacg gctcggcgaa cgtcaacaac actgccggaa tccgcgcggc caccgagcat
     9481 ctgcgctcgc tgcagtggtc ggagccgggc aacctctcga aggactggct cgcgcagtac
     9541 cagctcttcg gtggcggcaa cggcgtccag ggcggcacgt tcccgaacgt caccaagctc
     9601 gtcgttccgg ccaacaagga cctggacaag ggcttcggca agttcctccg cacggacgtt
     9661 cagccgggcc ggatcgtcaa cggcaagctc gttcgtcgcg tcgccatcca ctacaacatc
     9721 tcctacggcg tcaacgcgtt cgcgccgaag gcgaaccacg aggccgccta cctcttcctg
     9781 cagtgggcgg gcggtcgccg catgtacacg tacctgacgg cgagcccggg cggctaccag
     9841 gatccgcacc acacggcctc gttgaccgat ccgctcgtgg ttcagtcgta cggcgcccag
     9901 ccgatgcggg cgttcgcgca gatcgtcccg cggcaggtgc cgccgatcac gatccgtggc
     9961 gcggccgagt acaaccaggc cctcgacgag gagctgcaga agatgctgac caagcagcag
    10021 tctcccgagc gggcaatgcg gaacgtcgcg aaccggtggg acaagatcac cgaccggctc
    10081 ggctccggcc ggcaggcgaa ggccgtcaag cagagctacg tggccttccc gccgaaggga
    10141 cgctacggcc cgaacaagaa gatcacgtaa ggcggtcttc tccagcactg catgacggaa
    10201 accacagagc aacatccaac ccgacaggcg tccaaggggc gcctgtcgga cgttggtggc
    10261 cggaacgagt cggcggtctc cagcctcctg atcctgccgg ggcggctgat gctgctcttc
    10321 atcgtcctgt tcccggcagg ggtggcgatc tacctcggct tcacggagtg gacgcccacg
    10381 agcggcaccg acgtctgggg tgcctacaag ttctggcact ggttcgacgg ctac
//
LOCUS       DBNGNK010000191         5139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_964806,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000191 DBNGNK010000000
VERSION     DBNGNK010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_964806"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="AC150V_05575"
     CDS             <1..1056
                     /locus_tag="AC150V_05575"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR3762427.1"
                     /translation="LRVVADAEGRRLADPRMLADGTRALRALRRATPLLGAWATAAGP
                     RRVLLAGPRSFCAGVDRAIEIVERALSQRGAPVYVRKQIVHNAHVVGDLEQRGAVFVE
                     ELDEVPEGATVVFSAHGVSPQVKAEAKRRELDVIDATCPLVNKVHAEARNFAKSGRTI
                     FLVGHEGHEEVEGTTGEAPDSIRLVESVDDVAGIEVADPDQVAFLTQTTLAVDDTMVV
                     VDAIRERFPALVGPKSDDICYATQNRQQALKAVARESDLVLVVGSENSSNSQRLVEVS
                     EREGTRAYLVDDATEIDPSWLHGAGTVGVTAGASAPEVLVEQIVAELRGLGPVGVEQR
                     TITQETIEFGLPKEVNR"
     gene            1053..2069
                     /gene="hpnH"
                     /locus_tag="AC150V_05580"
                     /pseudo
     CDS             1053..2069
                     /gene="hpnH"
                     /locus_tag="AC150V_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014152779.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
     gene            complement(2055..2807)
                     /locus_tag="AC150V_05585"
     CDS             complement(2055..2807)
                     /locus_tag="AC150V_05585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762428.1"
                     /translation="MEIERSVCLVTGASSGIGAEVAKRLAGAGGRIVASGRDATRLAS
                     IAAATGGHAVAADLSRASEVERLSREAARPFGPVDVLVNNAGAGWAGAVDGLEPTDAE
                     QLVALNLLAPILLARALLPGMLERGGGHIVNVASIAGHVGAKGEAVYAGAKGGLVIFT
                     ESLRQELATTPVGVSVVSPGVIDTPFFARRGTPYTRRRPRPIPAAVVADAVLDAIVRN
                     RAVVIVPRWLTVPARLHGALPGLYRALATRFG"
     gene            2929..4356
                     /locus_tag="AC150V_05590"
     CDS             2929..4356
                     /locus_tag="AC150V_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3762429.1"
                     /translation="MARPRSKAPARSGGSTAQVKSKPRATSTPAARRAVLKRVKDEGI
                     EHILFWFTDIEGHLKSFAITPSELADALDDGMGFDGSSITGFNAIEESDMVAIPDPAS
                     FQLMPRPVDASGESIGAKVGRLMCDVVRPDGKPYEGDPRHVLRLALDRMRSMGFDAFN
                     VGPELEYFLFADNEGTETLDEGGYFAMTAQDAATEVRNDTIGALESMGIDIEYHHHEV
                     GPSQHEIDMRFDDALAMADKTLTYRMVVKEVAAWHGYYATFMPKPLFGENGSGMHTHM
                     SLFKNGRNQFFDAKDSHHLSPTAKSFIAGLLVHAREMTVVLAQWVNSYKRLVPGYEAP
                     VYVAWSQRNRSALIRIPLYKPGSEQATRAEIRCPDPACNPYLAFAALLHAGLEGIDKG
                     YELPGEMTTNLYALSHDERDERGIVALPESLGQAVAEFDGSELMRRALGEHIFPRYSE
                     LKWREWDEYRMQVSRWELERYLPAL"
     gene            complement(4540..4992)
                     /locus_tag="AC150V_05595"
     CDS             complement(4540..4992)
                     /locus_tag="AC150V_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762430.1"
                     /translation="MPELVAAIRPDDWNFPLFLHVLGAMILVGTVAAVGLAELTSSST
                     SVPAMLRRVGFWSLLLVGVPAYIVMRVGAEWLYEKEFGGSDDDPAWIGIGYITADAGA
                     LLLLISLVLAGFASRRNSSRLARVTGGIMIVLVVAWLVSVWAMGAKPG"
BASE COUNT          874 a         1761 c         1733 g          771 t
ORIGIN      
        1 ctgagagtgg tcgccgacgc ggagggccgg cgactcgccg atcctcgcat gctcgccgac
       61 ggcacgcgcg cgctgcgcgc gctgcggcgg gccacgccgt tgctcggggc ctgggcgacg
      121 gccgccggtc cgcgccgcgt cctgcttgcc ggcccgcgct ccttctgcgc gggcgtcgac
      181 cgcgcgatcg agatcgtcga acgggcgctc tcgcagcgcg gcgctccggt ctacgtgcgc
      241 aagcagatcg tccacaacgc gcacgtcgtc ggggacctcg agcagcgcgg cgcggtcttc
      301 gtcgaggagc tcgacgaggt gccggaaggc gcgaccgtcg tcttctccgc gcacggcgtc
      361 tcaccgcagg tgaaggccga ggcgaagcga cgggagctcg acgtgatcga cgccacctgc
      421 ccgctcgtca acaaggtgca cgcggaggca cggaactttg ccaagtccgg ccgaaccatc
      481 tttcttgtcg ggcacgaggg acacgaggag gtcgagggga caacgggcga ggctcccgac
      541 tcgatcaggc tcgtggagtc ggtcgacgac gtcgccggca tcgaggtggc cgaccccgac
      601 caggttgcct ttctcaccca gaccacgctg gcggttgacg acacgatggt cgtcgtcgac
      661 gccatccgcg agcggttccc cgccctggtc ggccccaagt cggacgacat ctgttacgcg
      721 actcagaacc gccagcaggc gctgaaggcg gtcgcccgcg agtccgacct cgtcctcgta
      781 gtgggctcgg agaactcgtc caactcacag cgcctcgtcg aggtgtcgga gcgcgagggg
      841 acgcgggcgt atctcgtcga cgacgcaacc gagatcgacc cgtcgtggct gcacggggcg
      901 ggtacggtcg gcgtcaccgc cggcgcctcc gcacccgagg tgctcgtcga gcagatcgtc
      961 gccgagctcc gcggtctcgg gcctgtcggc gtcgaacagc gtacgatcac gcaggagaca
     1021 atcgagttcg gcttgcccaa ggaggtcaac cgataaccgt cccgctccgc caatcgctcg
     1081 ctgtcggccg acatctcttc cggcagaaac tcgcccgccg ggacaagttc ccgctgctcg
     1141 tcgagctcga gccgctcttc cagtgcaacc tggcgtgctc gttctgcggg aagatccagt
     1201 acccggatca catcctccgc cagcgaatgc ccgtcgaccg ggcgatcgcg gcgatcgagg
     1261 aggcgggtgc gccgatggtc tcgatcgccg gaggcgagcc cctcgtgcat cccgaggtgc
     1321 atgtgatggt gcgcgagctg atcgcccgga agaagttcgt gtttctctgc tcgaacgcga
     1381 ttttgatgaa gaagaagatg cacctcttcg agccctcgcc gtacttcgcc tgggtcgtgc
     1441 atctcgacgg gatgcgcgag cggcatgacc agttcgtcga gcgcgagggc atcttcgaca
     1501 tggccgtcga ggcgatccag ctcgcaaagg accgcgggtt ccgcgtcaac acgaacacga
     1561 cgttcctcac cacggacacc ccgaagacgg tgcgcgaggt gctcgacttc atcaacgacg
     1621 agctcgaggt cgacgcgatg atgatctcgc cggcctacgc gtacgaaaaa gcgcccgacc
     1681 aggagcactt ccccggcgtc acgcaaacac gcaacctgtt ccgccaggcg ttcggcaacg
     1741 ggaaccgcaa gcgctggcgt ctgaaccaca cgccgctctt cctcgacttc ctggagggca
     1801 aggtcgactt cgagtgcacg gcctggggga ttccgagcta ctcgatcttc ggatggcagc
     1861 gcccctgcta cctcatggcg gacgggtaca cggagacgta ccgggagctc gtcgagacca
     1921 cagactggtc gaagtacgga cgcggccgtg acccgcgctg cgccaactgc atggcgcact
     1981 gcggttacga gcccacggca gtcctggcga cgacgtcgtc gctccgcgaa tcgttgcgcg
     2041 cggggcgcgc tcagctagcc gaagcgcgtc gccagcgctc gatacaggcc cggaagcgct
     2101 ccgtgcaggc gcgccggaac ggtcagccag cgcggcacga tcacaacggc ccggttccgg
     2161 acgatcgcgt cgaggacggc gtcggccaca acggccgcgg gaatgggtcg cggccggcgt
     2221 cgcgtgtagg gagtgccgcg ccgggcgaag aagggcgtgt cgatgacgcc cggactgacc
     2281 actgataccc cgaccggcgt cgtggccagc tcctgccgca ggctctcggt gaagatcacg
     2341 aggccgccct tggcgcctgc gtagacggcc tctcccttcg cgccgacatg gccggcgatc
     2401 gacgcgacat tgacgatgtg gccaccgccg cgctcgagca tccccggcag cagcgcgcgt
     2461 gcgagcagga tgggagccag aagattgagc gcgacgagtt gctcggcatc cgtcggctca
     2521 agcccgtcga cggcgccggc ccagccggcg ccggcgttgt tgacgaggac gtccacgggc
     2581 ccgaagggtc tcgccgcctc acggctcaga cgctccacct cgctcgcccg ggacaggtcc
     2641 gctgcaacgg cgtgcccgcc ggttgccgcc gcgatcgagg cgaggcgagt cgcgtcccgg
     2701 cccgaggcga cgatccgccc cccggccccg gcgagccgct tcgcgacctc agcgccgatc
     2761 ccgctcgatg ccccggtgac gagacagacg gagcgctcga tctccacgcg ccgagtgtat
     2821 tcggcgacgt ccgtcgcttg tcgctaagat ctcgacgtct gtgaggcgac gaggcctcgg
     2881 gcgcgcgagc gctcggggct ttttgtgttt tcgggaagga gaaatgcgat ggcaagacca
     2941 cggagcaagg ccccggcacg gagcggcggc tcgaccgcgc aggtgaagag caagccccgc
     3001 gcaacgtcca cccctgcggc cagacgggcg gtgctgaagc gtgtcaagga cgaggggatc
     3061 gagcacatcc tcttctggtt cactgatatc gaaggccatc tgaagagctt tgcgatcacc
     3121 ccttccgagc tcgcggatgc gctcgacgac ggcatgggct tcgacggctc gtctatcacc
     3181 ggcttcaacg cgatcgagga gtccgacatg gtcgcgatac ccgatccggc gagcttccag
     3241 ctgatgccgc ggccggtcga cgcgagcggg gagtcgatcg gcgccaaggt cggccgtctc
     3301 atgtgcgacg tcgtgcggcc cgacgggaag ccctacgagg gcgatccgcg tcatgtgctc
     3361 aggctggcgc tcgaccgcat gcgctccatg ggcttcgatg cgttcaacgt cggccccgag
     3421 ctcgagtact tcctcttcgc agacaacgag ggaacggaga cgctcgacga gggtggctac
     3481 ttcgcgatga ccgcccagga cgccgccact gaggttcgca atgacacgat cggcgcgctc
     3541 gagtcgatgg ggatcgacat cgagtaccac caccacgagg tcggcccgtc gcagcacgag
     3601 atcgacatgc gcttcgacga cgcgctggcc atggccgaca agacgctcac gtaccggatg
     3661 gtggtcaagg aggttgcggc ctggcacggc tactacgcga ccttcatgcc gaagccgctg
     3721 ttcggcgaga acgggtcggg gatgcacacg cacatgtcgc tgttcaagaa cgggcgcaac
     3781 cagttcttcg acgcgaagga ctcgcaccac ctgtcgccca ccgcgaagtc attcatcgcg
     3841 gggttgctcg tgcacgcgcg cgagatgacc gtcgtgctgg cccagtgggt caactcgtac
     3901 aagcggctcg tgcccggcta cgaggcgccc gtctacgtcg cttggtcgca gcggaaccgg
     3961 tctgcgctga tccggattcc gctctacaag cccggctcgg agcaggcgac ccgcgccgag
     4021 atccgctgcc ccgacccggc ctgcaacccg tacctcgcct ttgcggcgct cctccacgcc
     4081 ggactggagg ggatcgataa gggttacgag ctgcctggcg agatgaccac caacctctac
     4141 gcgctgtcgc acgacgagcg tgacgagcgg gggatcgtgg ccctgccgga gtcgctcggc
     4201 caggcggtcg cggagttcga cggctcggag ctgatgcggc gggccctcgg cgagcacatc
     4261 ttcccgcgct atagcgagct gaaatggcgc gagtgggacg agtaccggat gcaggtctca
     4321 cgctgggagc tggagaggta tctgcccgcg ctctgacagg tggggggagc cccgtcgggc
     4381 ttcccccact aaggaaacgc cctccacaac gaccacgggc cccccaccag ggagcccgcg
     4441 atcggccgtc agaagcagag ttcaccgctc gccttgcggc ttgctctgct agcgcaatca
     4501 tgcgccattc accagacgtt gtccagcgcg atcggcgcct cagcccggct ttgcgcccat
     4561 cgcccagacg gagacgagcc acgcgacgac gagcacgatc atgatcccgc ctgtgaccct
     4621 ggccaggcga ctgctgttgc gccgtgaggc gaacccggcg agaacgagcg agatcagtag
     4681 caggagcgcc ccggcgtctg ccgtgatgta accgatcccg atccacgccg ggtcgtcgtc
     4741 gcttccaccg aactccttct cgtacaacca ctccgccccg acgcgcatga cgatgtaggc
     4801 gggcacgccg acgagcagca gcgaccagaa ccccacgcgc cggagcatcg ccggaacgct
     4861 ggtcgacgac gaggtcagct cggccagacc gactgctgcg acagtaccga ccaggatcat
     4921 cgcgccgaga acgtgcagaa acagcgggaa gttccagtcg tcggggcgga tggcggcgac
     4981 gagctcaggc accgggcttc cagatcatga gaacgaggat ggccagggcg gcgaccgtac
     5041 tgatcgtgtg gagcgcgaat cccttcgggg tgcggacgag agcggccaac tcggcgtccg
     5101 gtccgtctcg tcccgcctcg gcgagctcgg cggcccgcc
//
LOCUS       DBNGNK010000192        11692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1323031,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000192 DBNGNK010000000
VERSION     DBNGNK010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 11692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11692
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1323031"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="AC150V_05600"
     CDS             <1..988
                     /locus_tag="AC150V_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762431.1"
                     /translation="RLDDLEEFLLELAPEEAFRPHRLWQTPLTGLHDYALPETIAARL
                     GPIEQLTEGLADSDVELVVCVTDVAEMDEEPGSHAFERTYSSRTSPPEIMGRAILASA
                     AISALVLPMRVGETIGTDGGWVRNYPLGHAYDNPDIRQIVGFRYVSRLPASSGENLTR
                     LRKRLERFRAVPPVRALIGELQEAEERQARGEPAHLPEMIVRLMRIAIARNTVVEERV
                     AAEKDASVAELETLRRDVLAIARRYALPGRRSKVTEEIEERFAAADFPFSHDRNLPTV
                     IVNGDPGDLALDPSFRAGIDWPVETKSALITLGYDLTDVALEQSDIAELEAA"
     gene            complement(1195..2361)
                     /locus_tag="AC150V_05605"
     CDS             complement(1195..2361)
                     /locus_tag="AC150V_05605"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3762432.1"
                     /translation="MFRSPVLQLAALAVVALLGGGAALAGAAAFGAFDSEGGTTTVVE
                     TVAGPASSTSAGSGSGLTVKEIYERSAPGVVQVTTRIGAQADSFGGQGQALGSGFVVD
                     ADGHIVTNYHVIEGADAISVSFSNRDTLDATLVGSDPSTDLAVLKVDAGPDALTVLPV
                     GDSDDVNVGDEVVAIGNPFGLERTATAGIVSALQRSVTAPNGFAIDEVIQTDAPINQG
                     NSGGPLFNTRGEVIGVNSQIETNSGGNVGIGFAVPSNTVKTVVSQILETGEVNRAYIG
                     IVARPIEPELARSVDLPTDAGLLVESVSEGSAADDAGLVAGAEQVVVAGESYRVGGDL
                     IVAAGGTEVGGVEELRDVLSEYKPGDEIALELYRDDKKITVTVKLGRQPASPQG"
     gene            complement(2467..3027)
                     /locus_tag="AC150V_05610"
     CDS             complement(2467..3027)
                     /locus_tag="AC150V_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AlbA family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR3762433.1"
                     /translation="MAGSVAVPAIVRTGASRYRTEEERSVLELIDGGESASLELKSTA
                     RVNLQTGKRDARMELIIAKTVAGFMNASGGTLLVGVADDGSIHGLWDDYETCQRPNRD
                     SFELWLSDYLGRVIGRNALGRLVVSFESIDGKDVCRIDVAASEKPVFVARPGGGRTAD
                     FYVRMGNATRQLLTDEALEYTATRWS"
     gene            complement(3246..3605)
                     /locus_tag="AC150V_05615"
     CDS             complement(3246..3605)
                     /locus_tag="AC150V_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005965905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3762434.1"
                     /translation="MSTIREQVEGIIRDEPVALFMKGTPDMVMCGNSERALRALVHAG
                     APVTAIDVLPDPRIRQELSAVSGWPTIPQVFVKGQLVGGADICEELGASGELKQTIDA
                     ALGDEHEQVVKTLAVAG"
     gene            complement(3602..3835)
                     /locus_tag="AC150V_05620"
     CDS             complement(3602..3835)
                     /locus_tag="AC150V_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HIR3762435.1"
                     /translation="MATDRLSDLLRQAFPEAGEIDVQDRTGGGDHFQVTVTSAEFNSL
                     PLLEQHRRVNAALSDLLADGTIHELRIRTRGTQ"
     gene            3853..4737
                     /locus_tag="AC150V_05625"
     CDS             3853..4737
                     /locus_tag="AC150V_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762436.1"
                     /translation="MRRHATFTLSLPERIVRAVAAALGGTVHETAGIVLPRFVRASRL
                     YEATAKNVLRVSIELVGGVEPPTPAATEFEPDPGKLAVRKAAGNVVELGSIAAFGFSP
                     LWLLAATADVMHGSRVYLDALVRELQAAKVLAEDAELETVDDLLAALEGTSGTAARLI
                     DIPPLELASLRQSLDDLRRDAGSLPSTVQLAALYRGLEATAAREERSLLAVSVGMGLA
                     FFNSARHVGRQHVLDPYSEDLRPLRDEGFGAYARRVGRPYTEAARSHFDPAQMSLTER
                     GIERLTRSADQGDGEPSD"
     gene            4746..7457
                     /gene="acnA"
                     /locus_tag="AC150V_05630"
     CDS             4746..7457
                     /gene="acnA"
                     /locus_tag="AC150V_05630"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR3762437.1"
                     /translation="MTHPDSFSARSRLKAGGTELEIFRLDALQDRYDIRRLPYTLRIL
                     LENVLRHEDGDAVTAADVEAVATWVADARPSREISFTPARVLLQDFTGVPAIVDLAAM
                     RDAMADLGGDPAKINPLLPVELVIDHSVQVDDYASRFAITRNAELEFQRNRERYAFLR
                     WGQGSFDSFEVVPPNTGIVHQVNLEFLARVVEERDGRAFPDTLVGTDSHTTMINGLGV
                     LGWGVGGIEAEAAMLGEAISMLVPEVVGFRLTGELPEGATATDLVLTVTQILRETGVV
                     GKFVEYFGRGVSSLPLADRATIGNMSPEYGATCGFFPVDDETLRYLRLTGRSTERVSL
                     VEAYCKENHLWHEPDDAPTYSQIVELDLSDVEPSLAGPRRPQDRVPLREAKHAFFSSL
                     PSFGVDANGGVDETVLESMDASDAPALAAPKKTSVAATLDDETFELDHGSVVIAAITS
                     CTNTSNPAVMVGAGLLAKNAVERGLSRRPWVKSSLAPGSKVVTEYYDKAGLTPFLEAL
                     GFHTVGYGCTTCIGNSGPLREEISRAIGDGDLVACAVLSGNRNFEARIHPEVKANYLA
                     SPPLVVAYALAGRMDVDLLNEPLGISAGGEDVYLADIWPSPAAVQETIAHAVGEEMFR
                     ATYADVYTGDPAWRELPVPTGDLFAWDPESTYVRQPPYFSGMTEALDPVQDITGARCL
                     VAVGDSVTTDHISPAGAIKPDSPAGKYLVNHGVERPDFNSYGSRRGNHEVMVRGTFAN
                     VRMRNQLVPGSEGTWTAHLPDGDEMTVFEAAQRYLAENVPLIVLAGKEYGTGSSRDWA
                     AKGPELLGVKVVIAESYERIHRSNLLMMGILPLQFLPGENRESLGLSGREEYSIVGID
                     NAEAAEVAVRADATEFRARVRLDTPRERAYVRHGGILKYVLRRLLAT"
     gene            7525..8868
                     /locus_tag="AC150V_05635"
     CDS             7525..8868
                     /locus_tag="AC150V_05635"
                     /inference="COORDINATES: protein motif:HMM:NF014218.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-cytosine permease family protein"
                     /protein_id="HIR3762438.1"
                     /translation="MESSGRGASKIGIEPVPQELRVLGAIDFGLLWGNLGVSLLVIVA
                     GALLVPALSLPQALLAILVGCAVGNLMLAAAAGIGAQAGVPAMALMRAPLGRRGSYLP
                     TALNVVQTLGWTTFELLVIATAAAALSNELFGFEAQWLWTLVFGALALLLTLLGPVGV
                     VRRVIRRAAIVVVPLALGYLTWWALDGANLGALWEQPGEGGLSTWQGADLVVGITVSW
                     IPLAADYTRFGRSARAAFWGTGLGYLIPDAWLLALGAIIVLSRDLSDPAALPPAIAGG
                     GFVALLALVALTAGETDEAFANAYSAAVSIQNIFPAAPLPVLLTLTTGLGTVGALVLG
                     FGSFESFLFLLGSFFVPLIAVLLADWLTAGRHYVGAAVLRGRSWRPGPIGAWIVGFVV
                     YQWLSPTGPSAWVDVVERLEPQNWGTGATLPSFLVSFGLAALAGLARGRRPVTIA"
     gene            8868..9689
                     /locus_tag="AC150V_05640"
     CDS             8868..9689
                     /locus_tag="AC150V_05640"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3762439.1"
                     /translation="MPRVAVVGNLVKDIVDAGDPRPGGVVFYVARAFAALEATNGVQL
                     VTRCAEADRELLLPPLEAFGIPVVWRPAGTTQTFRFHYEGDARVMYVDELGDQWTEDD
                     IRGWVGAAIADCDKIMLGALTRRDFTQDAVLALAEEGRRLYVDAQAIVRLYQTGPLAE
                     DRDVNPVVFSLIAALKLSDDEALTLVGSIEPDVLRALGVPEVVVTQGSKGALVVTRDV
                     AEHIPAVPVEGVVDPTGAGDTFWVAYLHHRDKGADPADAARRASEFVARMLTRSD"
     gene            9695..10093
                     /locus_tag="AC150V_05645"
     CDS             9695..10093
                     /locus_tag="AC150V_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3762440.1"
                     /translation="MLVATALGVSLVDIEDGQVYELDDEDFVARTPLDLDLPLLLAAD
                     RHGSRVVAVVERRPPLVVSDDAGATWREAGGGLPAGRDVAISPAHPDVVIFATESRLY
                     LSRDGGRFWKALELELHGITAVAWEDDRDV"
     gene            complement(10090..10932)
                     /locus_tag="AC150V_05650"
     CDS             complement(10090..10932)
                     /locus_tag="AC150V_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR3762441.1"
                     /translation="MDLRYVVVDVFTDVPLAGNQLAVFTDGRDVGDAMMQDLALELGF
                     SETVFVLPAEGEGDARIRIFTPRSEIPFAGHPTLGTAFVLSGPLQRSVIALETGSGLV
                     PVMHDRDESGRIVFGRMVQPIPTVRLFEQPERLFAALGVAGSMLPVEAYDNGIVHVFV
                     ALESESEVAGLQPDVRALLPFGLTGVNCFAGGGERWKTRMFSPLDGVGEDAATGSAAG
                     PLACHLARHGLIDWGTEIEISQGTEIGRPSTLYARAEGSGDAISAVEVGGSAVIVARG
                     AFRV"
     gene            complement(10945..11388)
                     /locus_tag="AC150V_05655"
     CDS             complement(10945..11388)
                     /locus_tag="AC150V_05655"
                     /inference="COORDINATES: protein motif:HMM:NF015669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR3762442.1"
                     /translation="MKQLLVLLAILVGFGLIVAGVVAWADDSGRSSKSSALVGPVWEW
                     EAFLGGDDSTLTPADPSDYTIEFSEEGSVSVEADCNSGSGTYEEDGGSLTIELGGVTL
                     ASCEPGSLSDRFLRDLGFVRTYVLEGRRLSLNLLADAGDMRFSAT"
     gene            complement(11413..>11692)
                     /locus_tag="AC150V_05660"
     CDS             complement(11413..>11692)
                     /locus_tag="AC150V_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR3762443.1"
                     /translation="AAFAIVFSVSGFFLWLGERDHRFLLDGAGTVHDAATYASVALVV
                     GHLYLALIHPTTRHALRGMMTGDVSEEWAERHHAKWVEELRRADSSSR"
BASE COUNT         1903 a         4001 c         3910 g         1878 t
ORIGIN      
        1 tcggctcgac gatctcgagg agttcctgct ggagctcgcc ccggaggagg cattccgccc
       61 acatcggctc tggcagacgc cgcttaccgg actccacgac tacgcacttc ccgagacgat
      121 tgctgcgcgg ctcgggccga tcgagcagct cacggaaggt ctcgccgaca gcgacgtaga
      181 gctcgtcgtc tgcgtcaccg acgtcgccga gatggacgaa gagccgggat cgcacgcctt
      241 cgaacgaacg tactcctcgc gcacatcgcc gccggagatc atggggcgcg cgattctcgc
      301 gtcggccgcg atcagcgcac tcgtgctacc gatgcgcgtc ggcgagacga tcggcaccga
      361 cggcggctgg gtgcgcaact acccgctcgg tcacgcgtac gacaaccccg acatcaggca
      421 aatcgtcgga ttccgctacg tctctcggct tccggcgtcg tccggggaga acctgacgcg
      481 actgcgcaag cgtctcgagc gattccgggc agttcccccg gtgcgcgcgc tgatcggaga
      541 gctgcaggag gccgaggagc gacaggctcg tggcgagccc gcccacctgc ccgagatgat
      601 cgtccgactg atgcgcatcg cgatcgccag gaacactgtg gtcgaggaac gcgtcgcggc
      661 cgagaaggat gcgtccgttg cagaactcga gacgctcagg cgcgatgtct tggcgattgc
      721 gagacgctat gccctgcccg gtagacgcag caaagtgacg gaagagatcg aagagcggtt
      781 cgccgctgcg gacttcccgt tctcccacga ccgaaacctg ccgacggtga tcgtcaacgg
      841 cgacccaggc gatctggcgc tcgaccccag cttccgtgca gggatcgact ggcccgttga
      901 gacgaagagc gctctcatca cgctcggcta cgacctgacc gacgtcgcgc tcgagcagag
      961 cgacatcgcc gagctagaag ccgcgtagac ctagaagccg cgcagacaga gcccaggtcg
     1021 aacgcggccg aaccgcttca cagctcgagc cggcggacgc gcggagaggg ggacgctccg
     1081 cgggtgggca tgcggccgcc ggccggagtt gcgagcgccg agccccctgg gcaggcagtc
     1141 gggggcccgg cgccgtgtgg aggacccggg gggacgtggg tcctaccggg acggctatcc
     1201 ctggggagaa gcgggctgcc gtcctagctt caccgtgacg gtgatcttct tgtcgtcgcg
     1261 gtacaactcg agtgcgatct cgtcgccggg cttgtactcg gacagaacgt cccggagctc
     1321 ctcgacgccg ccgacctcgg ttccgcctgc cgcgacgatg aggtctccgc cgactcgata
     1381 gctctcgccg gcgacgacga cctgctccgc gccggccacg agccctgcat cgtccgccgc
     1441 gctgccttcg gagaccgact ccaccaacag accggcgtcc gtgggaaggt cgaccgagcg
     1501 cgctagctcg ggctcgattg ggcgggcaac gataccgatg taggcgcggt tgacttcacc
     1561 cgtctcgagg atctgggaca cgaccgtctt gaccgtgttg gaggggacgg cgaagccgat
     1621 gccgacgttg ccgcccgaat tcgtctcgat ctgcgagttg actccgatca cctcgccgcg
     1681 cgtgttgaag agtggaccgc cggagttgcc ttggttgatc ggcgcatcgg tctggatgac
     1741 ctcgtcgatc gcgaagccgt tcggcgcggt gaccgaacgc tgaagtgcgc tgacgatgcc
     1801 ggctgtggcg gttcgctcga gcccgaacgg attgccgatc gcgaccacct cgtcgccgac
     1861 gttgacatcg tcggagtcac cgaccggcag gacggtgagg gcatccggac cggcgtcgac
     1921 tttcagaacc gcgagatcgg tcgacggatc cgatcccacc aacgttgcgt caagcgtgtc
     1981 gcgattcgag aagctgacgc tgatcgcgtc tgcgccctcg atgacgtgat agttggtcac
     2041 gatatgaccg tcggcgtcga cgacgaagcc cgagccgagc gcctggccct gaccgccgaa
     2101 gctgtcggcc tgggcgccga ttcgcgtggt cacctgcacg acgccggggg cactgcgttc
     2161 gtagatctcc ttgaccgtga gccctgagcc tgagcctgct gacgtcgagg acgcggggcc
     2221 ggcaacagtc tcgaccacgg tcgtcgtccc gccctcgctg tcgaaggctc cgaatgcggc
     2281 ggctccagcc agcgccgcac cgccgccgag gagggctacg acggcgagcg cggctagctg
     2341 gagaacaggg gaacggaaca tgcgacttcc tatctacaga ggccccggtg aaacaggggt
     2401 taggactggt tcagttcccg ttgcgctgga ttaagagggc gtgctcggac agcgcccgcg
     2461 tgtccttcaa ctccagcgag tagccgtgta ttcgagggcc tcgtccgtga gcagctggcg
     2521 cgtcgcgttc cccatccgca cgtagaagtc tgccgtccgc ccgccgcccg gccgcgcgac
     2581 gaaaaccggt ttctcactcg cggcgacgtc gatcctgcag acgtccttcc cgtcgatcga
     2641 ctcgaaactg actacgaggc gcccgagcgc gttgcggccg atcacacggc cgagatagtc
     2701 ggagagccac agctcgaagc tgtcgcggtt cggccgctgg cacgtctcgt agtcgtccca
     2761 gaggccgtga atggagccat cgtccgcgac gcccacgagc agcgtccctc cgctcgcatt
     2821 catgaagccc gccaccgtct tggcgatgat cagttccatc cgggcgtcgc gcttgccggt
     2881 ctggaggttc actcgcgcgg tggatttcag ttcgagcgaa gcgctctcac cgccgtcgat
     2941 cagctcgagg acgcttcgtt cctcctccgt gcgatagcgc gaggcccccg tgcggacgat
     3001 tgcaggcacc gccaccgagc cggccaccga ggcgacgatg cggatccagt acgcatcggc
     3061 accggccgcc tccgagatac cgaagccgag agcctgcgat gccgactacg acgatcgcgc
     3121 tcaaggagac gcgccgcggc ccgccgagga tcacgcgggc cagcaacccg gccgcgaggc
     3181 ccacgccgag gacggcgaag aagatcacgc cgcccggaga gcccttcgga ccgctccggc
     3241 ggcgatcacc cggctacggc gagtgtcttc acgacctgtt cgtgctcgtc acccagggcc
     3301 gcgtcgatcg tctgcttcag ctcacccgag gcgcccagct cttcacagat gtccgcaccg
     3361 ccgacgagct ggcccttgac gaagacctgg gggatcgtcg gccagccgga gacggcggac
     3421 agctcttgcc ggatgcgcgg atcgggcagg acgtcgatcg ctgtgaccgg cgcgcctgcg
     3481 tgaacaagag cgcggagcgc ccgctcggaa ttcccacaca tcaccatgtc gggcgtgcct
     3541 ttcatgaaca gtgcaaccgg ctcgtccctg atgatgcctt cgacctgctc gcgaatcgtg
     3601 ctcattgtgt ccccctggta cggattcgta actcgtggat cgtgccgtcg gccagcagat
     3661 cggacaacgc cgcgttgacg cgcctgtgct gctcgagcag cggcaggctg ttgaactccg
     3721 cgctcgtgac ggtcacctgg aaatgatcgc cgccgccggt gcggtcctga acgtcgatct
     3781 ccccggcttc gggaaacgcc tgccggagga gatcgctcag ccggtcggtc gccatcgagc
     3841 atgatgctag cgatgcgacg ccacgcgaca ttcacgctct ccctcccgga gcgcatcgtg
     3901 agagccgtcg ccgcggcgct cggcggcacg gtgcacgaga ccgcgggcat cgtgctgccg
     3961 cgcttcgtgc gcgcctcgag gctctacgag gcgaccgcga agaacgtgct ccgtgtcagc
     4021 atcgagctcg tcggtggcgt cgagccgccg acaccagccg ccacggagtt cgagcccgac
     4081 cctgggaaac tcgccgtgcg gaaagcggcc ggcaacgtcg tcgagctcgg ctcgatcgca
     4141 gcattcggct tctcgccctt gtggctgctc gccgcgaccg ccgacgtcat gcacggctcg
     4201 cgcgtctatc tcgacgccct cgtgcgggag ctacaggccg cgaaggtgct ggcggaggac
     4261 gccgagctcg agaccgtcga tgacctcctc gcggcactcg aaggcacttc gggaacagcg
     4321 gcgcggctga tcgacatccc gccgttggag ctcgcgtcgc tgcgccaatc gctcgatgat
     4381 ctgcgccgcg acgcgggcag cctgccgtcc accgttcagc tggcggctct ctacagaggt
     4441 ctcgaggcaa ccgcggcgcg ggaggagcgc tcgctgctcg cggtctccgt cggcatgggg
     4501 ctcgccttct tcaactctgc gcggcatgtg ggccggcagc acgtcctcga cccgtacagc
     4561 gaggatctcc gccctctgcg agacgagggc ttcggcgcct acgcgcgccg tgtcggtcgc
     4621 ccctacacgg aagcagcacg aagccatttc gatcccgcgc agatgtccct gaccgagcgc
     4681 ggcatagagc gactgacgcg gtcggcggac cagggcgacg gcgaaccgtc tgattgaatc
     4741 cgagcgtgac ccatcccgac agtttcagcg ctcgctcccg gctgaaagca ggcggtacag
     4801 agcttgagat cttccgcctg gacgccctcc aggaccgcta cgacatcagg cgcctcccgt
     4861 acacgctccg catcctgctc gagaacgtgc tgcggcacga ggacggcgat gccgtcaccg
     4921 cggccgatgt cgaggcggtc gcgacatggg tggcggacgc acggccgtca cgcgagatct
     4981 cgttcacccc cgcccgagtc ctgctgcagg acttcacggg tgtccccgcg atcgtcgact
     5041 tggcggcgat gcgcgacgcc atggcagatc tgggaggaga tcccgccaag atcaacccgc
     5101 tcctgcccgt tgagctggtg atcgaccatt ccgtccaggt cgacgactac gcgagccgct
     5161 tcgcgatcac gcgcaacgcc gagctcgagt tccagcgaaa ccgcgagcgc tatgccttcc
     5221 tccgttgggg acaagggtcg ttcgacagct tcgaggtcgt tccgccgaac accgggatcg
     5281 tgcaccaggt caacctcgag ttcctcgcac gagtcgtcga ggagcgggac ggccgcgcgt
     5341 tccccgacac gctcgtgggc accgactcgc acacgacgat gatcaacggc ctcggcgtgc
     5401 tcggttgggg ggtcggagga atcgaagccg aggcagcgat gctcggcgag gcgatctcga
     5461 tgctggtgcc cgaggtcgtc ggctttcggc tgaccgggga gctgcccgaa ggcgcgaccg
     5521 cgacagacct cgtcctgacg gtaacccaga tcctgcgtga gaccggcgtc gtggggaagt
     5581 tcgtggagta cttcgggcgt ggcgtctcga gcctcccgct ggccgacaga gccaccatcg
     5641 gcaacatgtc acccgagtac ggagcgacct gcggcttctt ccccgtggac gacgagacgc
     5701 ttcgctacct gcggctgacg ggacgaagca cggagcgggt ctcgctcgtc gaggcgtatt
     5761 gcaaggagaa ccatctctgg cacgagcccg acgatgcgcc aacctactca cagatcgtgg
     5821 agctcgacct gtccgacgtc gagccctcac tcgccgggcc tcgccgacca caggatcgcg
     5881 tgccgctccg ggaggccaag cacgccttct tctcctcgct cccgtccttc ggggtcgacg
     5941 ccaacggagg cgtggacgaa acggtcttgg agtcgatgga cgcgagcgac gcgcccgctc
     6001 tcgccgcacc caagaagacc tctgtcgcgg caaccctcga cgacgagacg ttcgagctcg
     6061 atcacggctc ggtcgtcatc gcggcgatca cgtcgtgcac gaacacgtcg aatcccgccg
     6121 tgatggtcgg cgcagggctg ctcgcgaaga atgcagtcga gcggggcctg tcgcgccggc
     6181 catgggtgaa gtcgagtctg gctccgggct cgaaggtggt caccgagtac tacgacaagg
     6241 ccggattgac ccccttcctg gaggcgctgg gcttccacac cgtcggctac ggctgcacga
     6301 cctgcatcgg gaactcgggg cctctacgcg aggagatctc gcgggcgatc ggcgacggcg
     6361 acctcgtcgc ctgcgccgtc ttgtccggca atcgaaactt cgaggctcgc attcacccgg
     6421 aggtgaaggc caactatctc gcctcgccgc cgctcgtcgt cgcgtacgcg ctggcagggc
     6481 gaatggatgt cgacctcctg aacgaacccc tcggcatcag cgccggcgga gaagacgtct
     6541 atctcgccga catctggccg tcgcctgccg cggtacagga gacgatcgcc cacgcggtgg
     6601 gcgaggagat gttccgcgca acctacgccg acgtgtacac cggcgacccg gcgtggcggg
     6661 agctgccggt gccgacaggc gacctgttcg cctgggatcc cgaatcgacc tatgtgcgtc
     6721 agccacccta cttctcggga atgacggagg cgctcgaccc agttcaggac atcacgggag
     6781 cgcgctgcct cgtcgcggtc ggcgattcgg tcaccaccga tcacatctca cctgcgggtg
     6841 cgatcaagcc cgactcgccc gcgggcaagt atctcgtgaa ccacggcgtc gaacgcccgg
     6901 acttcaactc gtacggctcc cgccgcggaa atcatgaggt gatggtgcgc ggcaccttcg
     6961 cgaatgtccg catgcgcaac cagctcgtgc cgggatcgga gggaacatgg actgcgcacc
     7021 tgccggacgg cgacgagatg acggtattcg aagcggcgca gcgctacctc gccgagaacg
     7081 ttccccttat cgtcctcgca ggcaaggagt acgggacagg ctcctcacgg gactgggccg
     7141 caaagggccc ggagctactc ggcgtaaagg tcgttatcgc ggagagctac gagcgcatcc
     7201 accgttcgaa tctcctgatg atgggaatcc tgccgctcca gttcctgccg ggggaaaacc
     7261 gcgagtcgct cgggctgagc gggcgcgagg aatactcgat tgtcgggatc gacaacgcgg
     7321 aggcggcaga ggtcgccgtc agagccgacg caacggagtt ccgggcaagg gttcggctcg
     7381 acacgccgcg cgagcgcgcg tacgtccgtc acggcggcat cctcaagtac gtcctgcggc
     7441 ggctcctggc cacctgacga cgaatatcga gtaagtaaca ggctgttacg tactcgtccg
     7501 cgacccgggt aggctccggc cgcgatggag agcagcgggc gcggcgcgtc gaagatcggc
     7561 atcgagccgg tcccgcagga actccgcgtc ctgggcgcga tcgacttcgg cctgctctgg
     7621 ggcaacctcg gcgtgtcgct cctggtcatc gtggcgggcg ctctgctggt gcctgccctg
     7681 tcgctcccgc aggcgctgct cgcgatcctc gtcggttgcg ccgtaggcaa cctgatgctc
     7741 gcggcagccg ctggaatcgg cgcccaggcc ggcgtcccgg cgatggcgtt gatgcgggcg
     7801 ccgctgggac gccgcggttc ctaccttccc accgcgctca acgtggtgca gacgctcggc
     7861 tggacgacgt tcgagctgct cgtcatcgcg accgccgcgg ctgctctttc gaacgagctg
     7921 ttcggcttcg aggcgcagtg gctctggacg ctcgtcttcg gcgcactggc gttgctcctg
     7981 acgctcctcg gcccggtcgg cgtggtgcgg cgggtcattc gtcgcgccgc catcgtcgtc
     8041 gtgccgctcg cgctcggcta cctcacgtgg tgggcgctcg acggcgcaaa cctgggcgcg
     8101 ctctgggagc agccgggcga gggtgggctt tcgacatggc aaggcgccga tctcgtcgtc
     8161 ggcatcaccg tctcctggat ccctctcgcc gccgactaca cacgcttcgg ccggagcgcg
     8221 cgggccgcgt tctggggaac cgggctcggc tacctgatcc cggacgcctg gctgctggcg
     8281 ctcggcgcga tcatcgtcct ctctcgtgac ctgtctgatc ccgccgcgct gccgccggcc
     8341 atcgccggcg gcggcttcgt cgcgctgctg gcgctcgtgg ctctcaccgc cggcgagacc
     8401 gacgaggcgt tcgcgaacgc gtactcggct gctgtctcga tccagaacat cttcccggcc
     8461 gcgccgctcc ctgtgctgct gacgctgacc accgggctcg gcacagtcgg cgcgctcgtg
     8521 ctcggcttcg ggagcttcga gtcgttcctc ttcctgctgg gctcgttctt cgtgccgctc
     8581 atcgcagtgc tcttggcgga ctggctgacg gccggtcggc actacgtcgg agctgcggtg
     8641 ctgagaggac ggagctggcg cccggggccc attggagcct ggatcgtggg cttcgtcgtc
     8701 tatcaatggc tgtcgccgac cggcccgagc gcctgggtcg acgtcgtcga gcgcctcgag
     8761 ccgcaaaact ggggcaccgg cgcgacactg ccgagcttcc tcgtctcgtt cgggctcgct
     8821 gccctggccg gcctggcccg tggccggagg ccggttacga tcgcttgatg ccgcgagtcg
     8881 ccgtcgtggg gaacctcgtg aaggacatcg tcgacgcggg agatccacga cccggcggcg
     8941 tggtcttcta cgtggcgcgg gctttcgccg cgctcgaggc gaccaacggg gtccagctcg
     9001 tcacccggtg cgccgaagcc gaccgcgagc tgctgctccc tccgctcgag gctttcggga
     9061 tacccgtcgt gtggcgaccc gcgggaacga cgcagacctt caggttccac tacgagggag
     9121 acgcgcgggt gatgtacgtc gacgagctag gcgatcagtg gaccgaggac gacattcgag
     9181 gatgggtcgg ggcagcgatc gccgattgcg acaagatcat gctcggcgcg ctcacgagac
     9241 gagacttcac ccaggatgcg gttctcgcgc tcgccgagga gggccgccga ctctacgtcg
     9301 acgcgcaagc catcgtgcgc ctctaccaga ccgggcccct cgccgaggac agagacgtga
     9361 acccggtcgt cttttcgctg atcgccgcac tcaagctgag cgacgacgag gcgctgacgc
     9421 tcgtcggcag catcgagccg gacgtgctca gggcgctggg cgtgcccgag gtcgtggtca
     9481 cgcaaggatc gaagggtgcg ctcgttgtca ctcgcgatgt cgccgagcac atacccgccg
     9541 tgccggtcga aggggtcgtc gaccccaccg gagcgggcga caccttttgg gtcgcgtacc
     9601 tccatcaccg cgacaaggga gccgacccgg ccgacgccgc gcggcgcgcg agtgagttcg
     9661 tcgctcggat gctcacccga agcgactagt gcacgtgctg gtcgccaccg cgctgggcgt
     9721 ctcgctcgtc gacatcgagg acgggcaagt ctacgagctc gacgacgaag acttcgttgc
     9781 aagaacaccc ctcgacctcg acctgccgct cctcctcgcc gccgatcggc acggctctcg
     9841 cgtcgtcgcc gtcgtcgagc ggcgcccgcc actcgtcgtt tccgacgacg ccggcgcgac
     9901 atggcgcgag gccggcggcg ggctccccgc cgggagggac gttgccatct ctcccgcgca
     9961 cccggacgta gtcatcttcg cgaccgaaag ccggctctat ctgtctcgcg acggaggccg
    10021 gttttggaag gcgctcgagc tcgagctgca cggcatcacg gcagtggcgt gggaggacga
    10081 tcgagatgtc tagacgcgga acgcaccgcg cgcgacgatc acggcagagc cgccgacttc
    10141 caccgccgag atcgcatcac cactgccttc ggcccgggcg tagagggtcg aaggtcggcc
    10201 gatctcggtg ccttgggaga tctcgatctc cgtcccccag tcgatcagtc cgtgtcgtgc
    10261 aaggtgacag gccagcgggc cggcggcgga gccggttgcc gcatcctccc ccacaccgtc
    10321 gagtggggag aacatgcgtg tcttccagcg ctcaccgccg ccggcgaagc agttcacgcc
    10381 ggtgagaccg aacgggagga gcgcccggac gtcgggctgg agccccgcga cctcgctctc
    10441 ggactccagg gcgacgaaca cgtggacgat gccgttgtcg tacgcctcga ccggaagcat
    10501 ggaaccggcg acgccgagcg ctgcgaacag gcgctccggc tgctcgaaca ggcgcaccgt
    10561 agggatgggc tgaaccatcc ggccgaacac gattcgacca ctctcgtcgc gatcgtgcat
    10621 gaccggaaca aggccgctcc cggtctccag cgcgatcacg cttcgttgga gcggaccgct
    10681 gagtacgaac gcggtaccca gagtgggatg gccggcaaag gggatctcgc ttcgaggcgt
    10741 gaagatgcga attcgcgcgt caccctcgcc ctcggcgggg aggacgaaga ccgtctcgga
    10801 gaagccgagt tccagagcga ggtcctgcat catcgcgtcg ccgacgtcac gcccgtcggt
    10861 gaacacggca agctggttgc cggcgagcgg aacgtcggtg aagacgtcga cgacgacgta
    10921 gcggagatcc atgcgacggg ccgcctacgt cgcgctgaag cgcatgtcgc cggcgtcggc
    10981 caggaggttc agggagagcc tccggccctc gaggacatac gtgcgcacga aaccgagatc
    11041 ccgcaggaac cggtcgctca gtgaacccgg ctcgcaggag gcgagcgtca ctcctccgag
    11101 ctcgatcgtg agcgatcccc cgtcctcctc gtaggtgcca gagccgctgt tgcagtcggc
    11161 ctcgacggac acactgccct cctctgagaa ctcgatcgtg tagtcggagg gatcagcagg
    11221 ggtcaatgtc gagtcgtcgc cgcccaggaa cgcctcccac tcccagacgg gcccgaccag
    11281 agccgacgat ttcgacgagc ggcccgagtc atccgcccaa gcgacaacgc cggcgacgat
    11341 gagcccgaag ccgaccagaa tcgcgagcag gaccagcagc tgcttcacgg cggcgaggct
    11401 accacctcgc ccctagcggc ttgagctgtc ggctcgccgt aactcctcga cccatttcgc
    11461 gtggtgacgc tcggcccact cctcgctgac gtcgccggtc atcatgccgc ggagagcgtg
    11521 gcgcgtcgtc ggatggatga gtgccaggta gaggtggccc acgaccagtg cgacggacgc
    11581 gtacgtggct gcatcgtgca ctgttcccgc accgtcgagc aggaagcggt ggtcgcgctc
    11641 accgagccag agaaaaaagc cggacacgga aaagacgatc gcgaacgccg cc
//
LOCUS       DBNGNK010000193         6276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_35011,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000193 DBNGNK010000000
VERSION     DBNGNK010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_35011"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="AC150V_05665"
     CDS             <1..694
                     /locus_tag="AC150V_05665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR3762444.1"
                     /translation="ANVAAWVAELGGSARFVGKIGDDEAGRLARGRLERCGAEVVGPV
                     AAVGSGIIVSLVSPEGERTMCSDRGVAPDLRPEELDASWFRDCDFLHVPGYSLLREPI
                     RSAAREAVELARADGAQISADLSSWSAIRDFGPERFRGELVELAPDVVFCNEDEQRVV
                     GGRIDAMVWILKLGRRGCSFDGEERAARPVADVVDPTGAGDALAAGWLVGGPDLALEA
                     AARCVAQVGAMP"
     gene            713..1597
                     /locus_tag="AC150V_05670"
     CDS             713..1597
                     /locus_tag="AC150V_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR3762445.1"
                     /translation="MSLLGLSDEVATALADGAAVVALETTLVAHGFPAPEGVAVGLES
                     ERRIRAAGAVPATVGVLAGLIRVGLSEPELERFTAEARKAGPRDLAACAVQGSVGATT
                     VGGTLAVAQRVGIRFLGTGGLGGVHRGFPQPPDVSADLGALARTAAVVVSSGVKSLLD
                     VPATSEVLETIGVPVLGYRTDTLPLFYSAAGGPRVSARVDEPGEAAGLAAAHWELGGA
                     GLLVGNAPPESIDVEDLVEEAVTEAARAGIGGQEVTPFVLSFLHERSGGRTMAVNRDL
                     IAENAALAAEIAVAHAAL"
     gene            1594..2622
                     /locus_tag="AC150V_05675"
     CDS             1594..2622
                     /locus_tag="AC150V_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007977205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enolase-like domain-containing protein"
                     /protein_id="HIR3762446.1"
                     /translation="MSLYDAVRDLPLDVERYELETPTLAVSSEWTRKTTVVRLQGAGE
                     EGLGEDVTYTAEEHDAVAARGPELPLAGTWTLDSFSQHLGSQTAFEHDAETASSPDYR
                     RWAYESAALDLALRQGGRSLAQAVGREPGAVSFVGSPRGGMGNWLEHYPGMRFKLDAQ
                     EDWTDSVIAELASTNSVDVVDLKGMYGESFGLRPDADLYRRVAEEMPEALLEDPQLTP
                     ETDSVLAPYRDRITWDAPIHSVADAESLPFAPKVLNCKPSRFATVRGLFDFYDYCSER
                     GIALYGGGQFELGPGRGQIQYLASLFHADAPNDVAPGVYNEGGPRPGLPTSPLTVGLR
                     PTGFLLLD"
     gene            complement(2608..3330)
                     /locus_tag="AC150V_05680"
     CDS             complement(2608..3330)
                     /locus_tag="AC150V_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762447.1"
                     /translation="MARPRYPKSSGDKPPPLPPEDRTVGQLVAETIRVYGESFFRILP
                     LGIPIAIVDQLQLEGTIAQRIAVLVVFAPLFTIAYAYACTIVGKRYLSRGEWLTALAA
                     GSLAFLPAAALFPWFALAAVLWLGLVSQVVPAAAIEGRSVAGAFRRGLELAKADFIHA
                     AGSLATLAIVFGLTRGVLGFLLRSQADNTIRVSIFLADVVIGPLLFVGAAMLYFDQAA
                     RAEHGSPSKRRRRRDAHSSVEE"
     gene            complement(3365..3919)
                     /locus_tag="AC150V_05685"
     CDS             complement(3365..3919)
                     /locus_tag="AC150V_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylase family protein"
                     /protein_id="HIR3762448.1"
                     /translation="MVLLVAATEIELCGHEGLVCGIGPVESAAATARVLALQRPDGVL
                     HVGLAGASGIEPGSLVIGAEAVYADLGAAIPVVDSSTPDPLLVTAIRAVLPAAPFVPI
                     RTSAAVSGAGSALSVADRAVEAMEGFGVLRACELAGVPAVEVRAIANAIGEDDRSLWR
                     IDDAIASLARALPRLLSAVGDDSG"
     gene            complement(3930..4394)
                     /locus_tag="AC150V_05690"
     CDS             complement(3930..4394)
                     /locus_tag="AC150V_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008358172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR3762449.1"
                     /translation="MKSYRSSWWRRAVNAIVRPLARAGLTGRRTHLLTVAGRTTGRPY
                     ATPVSLVELDGERWAVAPYGERNWVKNARAAGRVELRKGRREETLAVEEASGEEAVRV
                     LRRYYELTYVTRPFFDVTPDSPREDWLAEAPRHPVFRLVAEPVTGTKARTEQ"
     gene            complement(4398..>6276)
                     /locus_tag="AC150V_05695"
     CDS             complement(4398..>6276)
                     /locus_tag="AC150V_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR3762450.1"
                     /translation="LAQRIVNGEVPEGLKGKRLLSLDMGALIAGAKFRGEFEERLKAV
                     LNEVKASDGRIILFIDELHTIVGAGAAEGAMDAGNLLKPMLARGELHCIGATTLDEYR
                     KHIEKDAALERRFQPVFVGEPSVADTIAILRGLKERYEAHHGVRIRDAALTAAAVLSH
                     RYISDRFLPDKAIDLVDESASRLRMEIDSSPVELDEAERRVTQLEIELAAMAKETPAV
                     REPVERELAEAKSRRDELAARWAQEKDVLDRVKEITRRIDELKMEAEREERFGNLQRV
                     AEIRYGELPALEEELRERGEAAEAAEPMVKEEVDEDDIAAVVASWTGVPVDRLLEGEV
                     EKLIHMEERLHQRVVGQDAAIDAVSNALRRARSGLQDPNRPIGSFVFLGPTGVGKTEL
                     ARALAEFMFDDERAMVRLDMSEYMEKHTVSRLVGAPPGYVGFEEGGQLTEAIRRRPYS
                     VVLLDEIEKAHPDVFNVLLQLLDDGRLTDGHGRTVDFRNTVVIMTSNIRSADQLLEHF
                     RPEFVNRIDEIVTFEALTKEQLGEIVELQVATLQDRLAERGITLELTDNAKELLAEAG
                     WDPAFGARPLKRAIQRMLENPLALRLLEGEFGEGDTLRAYGDRGEVHFERAEAAEPAA
                     A"
BASE COUNT          896 a         2251 c         2202 g          927 t
ORIGIN      
        1 cgcgaacgtc gccgcgtggg tggccgagct gggaggcagc gcacgcttcg tcggcaagat
       61 cggcgacgac gaggccggcc ggctcgcccg tggccggctg gagcgatgtg gcgccgaagt
      121 ggtcggcccc gtcgcagctg tgggcagcgg catcatcgtc tcgctcgtgt cgccggaagg
      181 ggaacgcacc atgtgttccg accgcggcgt cgcgccggat ctccgcccgg aggagctcga
      241 cgcctcgtgg ttccgagact gcgattttct gcacgtgccc ggttactcgt tgctgcgcga
      301 gccgatccgg tcagccgccc gcgaggcggt ggagctcgcg cgcgccgacg gggcgcagat
      361 cagcgccgat ctctcgtcct ggagcgccat ccgcgacttc gggccggagc ggttccgagg
      421 ggagctcgtc gagctggcgc cggatgtcgt cttctgcaac gaggacgagc agcgagtcgt
      481 cggcggacgg atcgatgcca tggtctggat cctcaagctc ggtcggcgag gctgctcgtt
      541 cgacggcgag gagcgtgccg cgcgaccggt cgccgacgtc gtcgacccga ccggggccgg
      601 ggacgccctc gccgccggct ggctcgtggg tggccccgac ctcgctctcg aggcggccgc
      661 ccgctgtgtc gcgcaggtgg gcgcgatgcc gtagcccggt aggctcgccg gcgtgtcact
      721 gctcggcctc agcgacgaag tcgcgaccgc gctggccgac ggcgcggcgg tcgtagcact
      781 cgagacgacg ctcgtcgcac acggcttccc ggcgccggag ggcgtcgccg tcggcctcga
      841 gagcgagcgc cggattcgcg cggccggggc cgtccctgcg accgtggggg tgctcgccgg
      901 actgatcagg gtcggattgt ccgagcccga gctggagcgg ttcacggccg aagcccgcaa
      961 ggccgggccg cgtgacctcg cagcttgcgc cgttcagggc tcggtgggcg ccacgaccgt
     1021 cggcgggacc ctcgccgtcg cgcagcgtgt tgggattcgc ttcctcggga caggaggact
     1081 cggcggcgtc catcgcggtt tcccgcagcc gcccgacgtc tcagccgatc tcggcgcgct
     1141 ggcccgcacg gccgccgtcg tcgtctcgtc gggcgtgaaa tcgcttctcg acgtgcccgc
     1201 gacgagcgag gtgctcgaga cgatcggcgt gcctgtactc ggctaccgca ccgacaccct
     1261 gccgctgttc tactcggccg caggcggacc gcgggtctcg gcgcgagtcg acgaaccggg
     1321 cgaggcagcc ggcctcgccg ccgctcactg ggagctcggc ggcgccgggc ttctcgtcgg
     1381 caacgcgccg cccgagagca tcgacgtcga ggatctcgtc gaggaggccg tgacagaggc
     1441 ggcgcgagcc ggtatcggcg gtcaggaggt gacgccgttc gtgctctcct tcctccacga
     1501 gcgaagcgga ggccgtacga tggccgtgaa ccgcgacctc atcgccgaga acgcagccct
     1561 agccgccgag atcgccgtcg cccacgccgc gctatgagcc tctacgacgc cgtccgcgac
     1621 ctgccgctcg acgtcgagcg ctacgagctg gagacaccca cgctggccgt ctcgagcgag
     1681 tggacgcgaa agaccaccgt cgttcgactg caaggtgccg gcgaggaggg gctgggcgag
     1741 gacgtcacgt acacggcgga ggaacacgac gccgtggccg cacgcggacc ggagttgcct
     1801 cttgccggaa cgtggacgct cgactcgttc tcacagcatc tagggagcca gacggcgttc
     1861 gagcacgacg ccgagaccgc gtcgtccccc gactaccgcc gctgggccta cgagagcgcc
     1921 gcgctcgacc tggcgttgcg ccaggggggt cgctcgctcg cgcaggcggt cggccgcgag
     1981 ccgggcgccg tctccttcgt cggctccccc cgagggggca tgggcaactg gctcgagcac
     2041 tacccgggca tgcgcttcaa gctcgacgcc caggaggact ggaccgactc cgtgatcgcc
     2101 gagctcgcct cgacgaactc ggtggacgtg gtcgacctga aggggatgta cggcgagtcg
     2161 ttcgggctgc gccccgacgc cgacctctac cgtcgggtcg ccgaagagat gcccgaggcg
     2221 ctgctggaag acccgcagct cacgcccgag accgacagcg tcctggcgcc gtaccgagac
     2281 cggatcacgt gggacgcccc gatccattcc gtcgccgatg cggagtcgct tcctttcgcc
     2341 ccaaaggtgc tcaactgcaa gccgtcgcgc ttcgcgaccg tacgagggct gttcgatttc
     2401 tacgactact gcagtgagcg gggcatcgcg ctctacggcg gcggccagtt cgagctcggc
     2461 cccggccgcg ggcagatcca gtatctcgcc tccctgttcc atgccgacgc accgaacgac
     2521 gtcgcgcccg gcgtctacaa cgagggcggc ccacgccccg gcctcccgac gagcccactc
     2581 acggtggggc tccggccgac ggggttccta ctcctcgact gaggagtggg cgtctcgtct
     2641 gcgtcgccgc ttggagggcg agccgtgctc ggcgcgtgca gcctggtcga agtagagcat
     2701 ggcggcgccg acgaagagaa gtggtccgat caccacgtcg gcgaggaaga tcgagacgcg
     2761 aatcgtgttg tcggcctgcg agcgcagcag gaacccgagc acgccgcgtg tgaggccgaa
     2821 cacgatcgcc agcgtcgcca atgagcctgc ggcgtgaatg aagtcggcct tcgccagctc
     2881 caggccacgc ctgaacgcgc ccgcgacgga gcgaccctcg atcgccgccg ctggaacgac
     2941 ctgggacacg agccccagcc agagcaccgc cgcgagcgcg aaccacggaa agagcgcggc
     3001 ggcgggcagg aaagccagtg acccggctgc gagcgccgtc agccattcgc cgcgcgacag
     3061 gtatcgcttg ccgacgatcg tgcatgcgta cgcataggcg atcgtgaaca agggggcgaa
     3121 gacgacgagc acggcgattc gctgcgcgat cgtcccctcc agctgcagct ggtcgacgat
     3181 cgcgatcggg attccgagcg ggaggatgcg gaagaagctc tcgccgtaga cccgaatggt
     3241 ctcggcgacg agctggccga ccgtgcggtc ctcgggcggg agcggaggcg gcttgtcgcc
     3301 gctcgacttc gggtaacggg gccgcgccac cagtcggacg gtaccgctgc aaacgggacg
     3361 gggctcagcc tgaatcgtca cccacggcgg acagcaggcg cggcagcgcc cgcgcgagcg
     3421 aggcgatcgc gtcgtcgatc cgccacaggc tgcggtcatc ctcgccgatc gcgttggcga
     3481 tcgcccggac ctcgacggcc gggacgccgg ccagctcgca ggcgcgaagc acgccgaacc
     3541 cctccatcgc ctcgacggcc cgatcggcga ccgacagggc gctgcccgca ccggagacgg
     3601 ccgcgctcgt cctgatcggg acgaacgggg ctgccggcag taccgcccgg atcgccgtga
     3661 cgagcagggg atccggcgtg ctcgagtcca ccacggggat ggcggcgccg aggtctgcgt
     3721 agacggcctc tgcgccgatg acgagcgagc ccggctcgat tccactcgcg ccggcgagcc
     3781 cgacgtggag cactccgtcc gggcgctgca gtgcaagcac ccgcgctgtc gccgcggccg
     3841 actcgaccgg cccgatgccg cagacaagcc cttcgtgccc gcaaagctcg atctccgtcg
     3901 ccgcgacgag cagcaccatg cgggaaacgc tactgctcgg tgcgggcttt cgtccccgtc
     3961 accggctcgg cgacgagacg gaagacggga tggcggggcg cctcggcgag ccagtcctcg
     4021 cgcggcgagt cgggtgtgac gtcgaagaag ggcctggtca cgtacgtcag ctcgtagtag
     4081 cggcgcagga cgcgaaccgc ctcctcgccg ctcgcctcct ccactgcgag cgtttcctca
     4141 cgacgcccct tgcgcagctc gacccggccg gcggcacgcg cgttcttcac ccagttgcgc
     4201 tccccgtagg gtgcgacggc ccagcgctcc ccgtcgagct cgaccagcga gacgggggtc
     4261 gcgtagggcc ggccggtcgt gcggcccgcg acggtcagca gatgcgtacg gcgaccggtg
     4321 aggccggcgc gggccagggg gcggacgatc gcgttcaccg cccgccgcca ccagctcgat
     4381 cggtacgact tcacgcgtca ggcggccgcc ggctcggctg cttccgcgcg ctcgaagtgc
     4441 acctcgccgc gatcgccgta cgccctcaag gtgtcgcctt cgccgaactc tccctcgagc
     4501 agccgcagcg caagggggtt ctccagcatg cgctggatcg cccgcttcag cgggcgggcg
     4561 ccgaaggccg gatcccagcc cgcctcggcc aggagctcct tcgcgttgtc ggtcagctcg
     4621 agcgtgatcc cacgctctgc gagccgatcc tgcagcgtcg ccacttgcag ctcgacgatc
     4681 tcgccgagct gctccttcgt cagcgcctcg aacgtcacga tctcgtcgat ccggttcacg
     4741 aactcgggcc ggaagtgctc gaggagctga tccgccgagc ggatgttcga ggtcatgatc
     4801 acgacggtgt tgcggaagtc gacggtgcgt ccgtgaccgt cggtcaaccg cccgtcgtcg
     4861 agcagctgca gcaggacgtt gaacacgtcc gggtgcgcct tctcgatctc gtcgagcagg
     4921 acgacgctgt agggccgtcg gcggatcgcc tcggtgagct gcccgccctc ctcgaagccg
     4981 acgtagccgg gcggcgctcc caccaggcgc gagaccgtgt gcttctccat gtactccgac
     5041 atgtcgaggc gcaccatcgc ccgctcgtcg tcgaacatga actcggcgag agcccgggcc
     5101 agctcggtct tgcccacgcc cgtcggtccg aggaagacga acgagccgat cggccggttc
     5161 ggatcctgga ggccggatcg ggcgcgccgc agcgcgttcg acaccgcgtc gatggcggcg
     5221 tcctggccga cgacgcgctg gtgcagacgc tcctccatgt ggatgagctt ctcgacctcg
     5281 ccctcgagga gccggtccac ggggacacct gtccaggagg ccacgacggc cgcgatgtcg
     5341 tcctcgtcga cttcctcctt gaccatcggc tcggctgcct ccgcggcctc gccccgctcg
     5401 cgcagctcct cctcgagcgc cggtagctcc ccgtagcgga tctctgccac ccgctggagg
     5461 ttgccgaagc gctcctcgcg ctcggcctcc atcttcagct cgtcgatgcg ccgggtgatc
     5521 tccttcacgc ggtcgagcac gtccttctcc tgggcccagc gcgccgcgag ctcgtcgcgg
     5581 cgtgacttcg cctcggccag ctcgcgctcg accggctcgc gcacggcggg ggtctccttc
     5641 gccatcgccg ctagctcgat ctcgagctgg gtcacgcggc gctccgcctc gtcgagctcg
     5701 acgggagagg agtcgatctc catccgcagg cgagacgccg actcgtcgac gaggtcgatc
     5761 gccttgtcgg gcaggaagcg gtcggagatg tagcggtggg acaggaccgc ggcggcggtc
     5821 agcgccgcgt cgcggatgcg cacgccgtgg tgcgcctcgt accgctcctt cagtccccgg
     5881 aggatcgcga tcgtgtcggc aaccgacggc tcgccgacga agaccggctg gaaacggcgc
     5941 tccagcgctg cgtccttctc gatgtgcttg cggtactcgt ccagcgtggt cgcgccgatg
     6001 cagtgcagct cgccgcgcgc cagcatgggc ttgagcaggt taccggcgtc catcgcgccc
     6061 tcggcagcgc ccgcgcccac gatcgtgtgc agctcgtcga tgaacaggat gatgcgcccg
     6121 tcactcgcct tgacctcgtt gagcacggcc ttgagccgct cctcgaactc gccccggaac
     6181 ttcgcgccgg cgatgagggc gcccatgtcg agcgacagca gccgcttgcc cttgaggccc
     6241 tcgggcacct cgccgttgac gatccgctgg gcgagc
//
LOCUS       DBNGNK010000194         2724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1323124,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000194 DBNGNK010000000
VERSION     DBNGNK010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1323124"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..161
                     /locus_tag="AC150V_05700"
     CDS             6..161
                     /locus_tag="AC150V_05700"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3762451.1"
                     /translation="MGIPPTGKSVDVQLIDIVRFGADGLALEHWGVFDALTMMQQLGL
                     VPEDPSA"
     gene            complement(193..1557)
                     /locus_tag="AC150V_05705"
     CDS             complement(193..1557)
                     /locus_tag="AC150V_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009148803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR3762452.1"
                     /translation="MGDTVKYTLEESSLPRAWYNIASDLPEPAPPVLNPGTGEPVGPD
                     DLAPLFPMEIIMQEVSTEPRIEIPEEVRDVYRQWRPTPLYRARRLERALDTPARIFYK
                     YEGVSPTGSHKPNTAVAQAFYNKREGVKRITTETGAGQWGSSLAFAGALFDIEIDVFM
                     VRVSFDQKPYRRAMMEAFGARCVASPSDETQSGRAILAESPDSNGSLGIAISEAVEVA
                     AQDDSAKYALGSVLNHVLMHQTVIGEESLLQMEQADAYPDVLVGCTGGGSNFSGLAFP
                     FIGNKLRGGPDVRVVAVEPAACPTLTRGEYAYDFGDTGHLTPLVKMHTLGSTFVPPGF
                     HAGGLRYHGMAPLISHLHNLGLIEATAYTQTTVFDAGVEFARAEGILPAPEANHAVKG
                     AIDEALRCKEEGVSETILFNLCGHGHFDVGAYIDYFAGKLEDSAYDEAELAMALAGLP
                     SVGG"
     gene            complement(1655..>2724)
                     /locus_tag="AC150V_05710"
     CDS             complement(1655..>2724)
                     /locus_tag="AC150V_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3762453.1"
                     /translation="ETRPPSASKARVLAENARFMMLAADFGPAVSTGREALTLAERFE
                     RLDVQAEALINIGTSRFMLGDVNEGFGELERAVAIAKEIRAPTIIHRALNNLMDGRLR
                     LGELEECSRLLDEAIESNERFGYEGGLRWTRGEQVFLTELRGDWPRAEVLADDFLAWI
                     DAGHAHYLESTCRFTRARLARARGDLERDDRETERAVELGREARDLQALMPALVERGR
                     FLVGQGRMAEARALADELSFSFAREAHPTTVRAAWLLTDLGRADQLRDMLEASSDTAW
                     ARAASAIVAADHTRTANILGDIGAPGEEAYARLRAAETFVVEGRRAEADEQLQSALGF
                     FHSIEATAYVKQSEALLAATA"
BASE COUNT          382 a          944 c          956 g          442 t
ORIGIN      
        1 agctcatggg cattccgccg acggggaaga gcgtcgatgt gcagctgatc gacatcgtgc
       61 gcttcggcgc cgacggcctc gctctcgagc actggggtgt cttcgacgcg ttgacgatga
      121 tgcagcagct cggcctcgtc ccggaggacc cttccgccta gcgggcgtcg tgccgcgcga
      181 accgaagcgc ggctacccgc cgacggaggg cagccccgcg agcgccatcg ccagctcggc
      241 ctcgtcgtag gcgctgtcct ccagcttgcc ggcgaagtag tcgatgtacg cgccgacgtc
      301 gaagtggccg tggccgcaga ggttgaagag gatcgtctcg gagacgccct cctccttgca
      361 acgcagcgct tcgtcgatcg cgcccttgac cgcgtggttc gcctcgggcg cgggcaggat
      421 cccctcggca cgtgcgaact ccacgcctgc atcgaacacc gtcgtctggg tgtaggcggt
      481 cgcctcgatc aggccgaggt tgtgcaggtg gctaatcagc ggcgccatgc cgtggtagcg
      541 caagccaccc gcgtggaagc cgggcggcac gaaggtcgat cccagcgtgt gcatcttcac
      601 gagcggtgtg aggtggccgg tgtcgccgaa gtcgtaggcg tactcgccac gcgtcagcgt
      661 cgggcaggcc gccggctcga cagcgacgac ccgcacgtcg ggcccacctc gcagcttgtt
      721 cccgatgaag gggaaggcga gtccggagaa gttgctccca ccgccggtgc agccgacgag
      781 gacgtcgggg tacgcgtccg cctgctccat ctgcagcagc gactcctcgc cgatcaccgt
      841 ctggtgcatg agcacgtggt tgagaaccga cccgagcgca tacttggcgc tgtcgtcctg
      901 cgcggcgacc tcgaccgcct cggagatggc gatgccgaga ctgccgttcg agtccgggct
      961 ctcggcgagg atcgcgcggc cggactgcgt ctcgtcggac gggctcgcga cgcaacgcgc
     1021 gccgaacgcc tccatcatcg cgcggcggta cggcttctgg tcgaagctga cgcgcaccat
     1081 gaagacgtcg atctcgatgt cgaagagcgc tccggcgaag gcgagcgacg atccccactg
     1141 accggccccc gtctcggtcg tgatccgctt gacgccctcg cgcttgttgt agaacgcttg
     1201 tgcgaccgcg gtgttcggct tgtggctgcc ggtgggcgag acgccctcgt acttgtagaa
     1261 gatccgcgcg ggtgtatcga gtgcgcgctc cagtcgccgt gcccggtaga ggggcgtggg
     1321 ccgccactgc cggtagacgt cgcggacctc ttccgggatt tcgatccgcg gctcggtgga
     1381 gacctcctgc atgatgatct ccatcgggaa gagcggggcc aggtcgtcgg ggccgaccgg
     1441 ctcgccggtg cctgggttga ggacgggcgg cgccggctcc ggcagatcgg acgcgatgtt
     1501 gtaccaggcc cgcggcaggc tgctctcttc tagcgtgtac ttgacggtgt cgcccacggc
     1561 gctcctcctc gcttcgacct cgggtgtccg agtttacgcc gctcgcgctc cgtgcatcga
     1621 cgggatccgg tcgcggcggg ccgcctagcc ccgctcaggc cgtagcggcg agcagtgcct
     1681 cgctctgctt gacgtaggcg gtcgcctcga tcgagtggaa gaacccgagc gccgactgga
     1741 gctgctcgtc ggcctccgcc cgcctgccct cgacgacgaa ggtttcggcg gcccgcagcc
     1801 gagcgtaggc ctcctccccg ggcgctccga tgtccccgag gatgttcgcg gtgcgcgtgt
     1861 ggtcggcagc gacgatcgcc gacgctgcac gggcccacgc cgtgtccgaa gacgcctcga
     1921 gcatgtctcg aagctggtcg gcccggccga ggtcagtgag gagccaggcc gcgcggacgg
     1981 tcgtcggatg cgcttcgcgc gcgaacgaga aagagagctc gtcggcgagt gcccgcgcct
     2041 cggccatgcg gccctgaccg acgaggaagc ggccgcgttc cacgagcgca ggcatcagcg
     2101 cctggaggtc acgggcctca cggccgagct cgaccgcacg ctccgtctcc cggtcgtccc
     2161 gctcgaggtc gccgcgggcg cgggcgagcc gggctctcgt gaagcggcac gtggactcga
     2221 ggtagtgggc gtggcccgcg tcgatccagg cgaggaagtc gtcggcgaga acctcggcac
     2281 gcggccagtc gccgcgtagc tcggtgagga agacctgctc cccccgggtc cagcgcagcc
     2341 cgccctcgta gccgaaccgc tcgttggact cgatcgcctc gtccagcagg cgcgagcact
     2401 cctcgagctc gcccagccgc aggcggccgt ccatgagatt gttgagtgcc cggtggatga
     2461 tcgtcggcgc gcggatctcc ttggcgatgg cgaccgcacg ctcgagctcg ccgaatccct
     2521 cattgacgtc gccgagcatg aagcggctcg tcccgatgtt gatgagcgcc tccgcctgca
     2581 cgtccaggcg ctcgaagcgc tcggcgaggg tcagcgcctc ccgccccgtg ctgacagccg
     2641 gcccaaagtc cgccgcgagc atcatgaatc gtgcgttctc ggcgagcacc cgtgccttcg
     2701 acgcgctcgg cggccgcgtc tcga
//
LOCUS       DBNGNK010000195         7766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1466786,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000195 DBNGNK010000000
VERSION     DBNGNK010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1466786"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="AC150V_05715"
     CDS             <1..305
                     /locus_tag="AC150V_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762454.1"
                     /translation="TPAFAAALDRALDVTATAEQWIRRRGDVELVSPAALGVVCFRRR
                     DGRDDEAIARLNRDGLGHVSSTQIDGSTVSRLCVNGFRTRWRDAEQVLEKLAGPLD"
     gene            complement(307..849)
                     /locus_tag="AC150V_05720"
     CDS             complement(307..849)
                     /locus_tag="AC150V_05720"
                     /inference="COORDINATES: protein motif:HMM:NF039728.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HIR3762455.1"
                     /translation="MSIGPIQMVTIGFEGEQPGEEVRAELRKLRRSGLIRVIDLLFVA
                     KRDDGELISLQSTDFDEDGMRELGAWVGGLIGLGAAGSEGAHVGAALGAEALGREVLE
                     ASTGDIVDIGAMIPPGTSAAVVLFEHRWALPFRDAVLRAGGRALGDAWIRIDDLIAIG
                     AEYAEQIEAEGDEFDKGAAA"
     gene            complement(953..1717)
                     /locus_tag="AC150V_05725"
     CDS             complement(953..1717)
                     /locus_tag="AC150V_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3762456.1"
                     /translation="MLLNTYEWGDAAGQAVVCLHGVTGHGRRFRRLAETTLARHRVVG
                     VDLRGHGFSGWEPPWDIETHVADLVETADALGVHSATWIGHSFGGRLVVELADTVPAR
                     VERAVLLDPALYVEPAVSAEQAEGSRPDASFATAEDAIEAKLSSGSYYSTPAEFLDEE
                     AEQHLVQHDDGRLRWRYSQCSVITAFSEMSTEPPEWASCPTLVLLGERSWISVDVPAA
                     PHVESATLPGGHSILWDDFDQASAAIARFIEEGGTT"
     gene            complement(1708..2664)
                     /locus_tag="AC150V_05730"
     CDS             complement(1708..2664)
                     /locus_tag="AC150V_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006941800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3762457.1"
                     /translation="MQAVRVHEGGELRFEEIPDPEPEAGEVLVELRCAGLNRRDLLVS
                     KGVYPYPLPLVPGSDGAGIRRDTGDEVVILPSLRWGDGEDVGGPDFEVLGGPTDGTYA
                     ELVAVPAENCYPKPGHLSWEEAAAFPLAALTAYRAVFTRGGLREGETVLVLGAGSGVS
                     TFAIQLAVHEGARVIVTSSSDEKLDRARELGADEGVNYAHGDWVAAVKELGGADLVVD
                     SVGTTWPDSLACCRPAGRVVVFGATGGTEATLQVRPVYFNQLSLLGTMIGSPRDFAGL
                     LRVMGEAPWRPVIDSVHALAGAEAAQGRLAGDHFGKIILTCS"
     gene            complement(2664..3062)
                     /locus_tag="AC150V_05735"
     CDS             complement(2664..3062)
                     /locus_tag="AC150V_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3762458.1"
                     /translation="MGALKSAAEIDRQEMDWGVFATVSSPEDGLSQIVTIEATFLPGQ
                     CHDFHRHPGQEEVIYVIEGMLEQWVEQEKRVLTAGDAALIPSSAVHATFNDTDQPAKI
                     IAILSPAIGDDGYGLEDLSTEEPWASLRTG"
     gene            complement(3062..3889)
                     /locus_tag="AC150V_05740"
     CDS             complement(3062..3889)
                     /locus_tag="AC150V_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate hydrolase family protein"
                     /protein_id="HIR3762459.1"
                     /translation="MTRQQALARLRGQIEQGSPIIGAGAGTGLSAKCAEGGGADLIII
                     YNSGRYRMGGRGSLSGLMPYGDANAIVVDMAAEVLPIVSETPVLAGVCGTDPFRLMPV
                     FLEELKRIGFSGVQNFPTVGLIDGTFRQNLEETDMGYGHEVDMIRAAHELDLLTSPYV
                     FDADDARAMAEAGADVLVPHMGLTTGGSIGAETAKTLDDCVPLIQEMHDVAKRVNPEI
                     IVLCHGGPIAEPDDAAYVLERTEGVVGFFGASSMERLPTEVAMTENMKRFKAIPVSA"
     gene            complement(3890..5104)
                     /locus_tag="AC150V_05745"
     CDS             complement(3890..5104)
                     /locus_tag="AC150V_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HIR3762460.1"
                     /translation="MPTVVLVGTLDTKGKEYEFLAGRVREHGVDVVLVDVGVFEPTVA
                     PDIGNEEVAAAVGADVKALAEAGDRGAAVATMAEGAAVVLKRLSDEGRLDGALGLGGS
                     GGSSLATHAMRQLPIGVPKLMVSTVASGDTSPYVGSVDVTMMYSVVDIAGVNEISAKI
                     MTNAAGMIAGAVKGTVPDLGEKKPLIVASMFGVTTPAVTTARERLEELGYEVLVFHQT
                     GAGGGSMEELIKAGFITGSLDVTTTEFCDELVGGVFPAVPERLESAGAAGIPQVVSLG
                     ALDMVNFGPRDTVPEKFESRNLYVHNPTVTLMRTTPEECKQLGGRIAAKLNAATGPTV
                     LFVPLKGVSMIAVEGQVFHDPEADEVLFQAVRDTIDTENVEVREIEADVNDPEFALAM
                     ANRLHELIQGAS"
     gene            complement(5183..6109)
                     /locus_tag="AC150V_05750"
     CDS             complement(5183..6109)
                     /locus_tag="AC150V_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762461.1"
                     /translation="MRKGWLLVLGLVGLFALVASGCGGGGKSADAGDLLVVSTRDGDY
                     ALFAVDAEGNGEARVTEDRGDPSTTEGLAYQIDPDWSPDGTKIAFASARDGSFDIFVM
                     DADGTNARNISNSDAQDGRPTWSPDGSQIAFQRGGGHIFVMDSDGSDVRRLTDDLAPE
                     IEPAWSPDGEWIAYARRTPGTDTIEIWIAKPDGSVRRQLTTLDAKSYGPAWAPDGKTV
                     AFAANRGGVRFDIYTIGVDGKELRRAVRSAEDAFEPAWSPDGRLLAFSRGGAIVTVDR
                     DGNEVEITDAANNDSSPTWRPGLDGAEDGGDG"
     gene            complement(6119..6922)
                     /locus_tag="AC150V_05755"
     CDS             complement(6119..6922)
                     /locus_tag="AC150V_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762462.1"
                     /translation="MSDPGTTEASAGQPAAEPAGRAVLEIEELSAFYGSAQALENVTF
                     AMGRESIAIVGRNGMGKTTLCNAIMGISPPNARGSIRFEGRELVGGQSYKIARQGLGY
                     VPQGRRLFPSLTVDEHLRMAARGAENGEGEEWTRRRVYDLFPRLAERKRNGGAQLSGG
                     EQQMLAIGRALVTNPRLLVMDEPSEGLAPAIIESLIETFQQLEQEGLGILLIEQNLGV
                     ATALAERQLIMVAGQIAHETTAAKLAGDSELQRRYLGVEPLAADKASDG"
     gene            complement(6915..7700)
                     /locus_tag="AC150V_05760"
     CDS             complement(6915..7700)
                     /locus_tag="AC150V_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762463.1"
                     /translation="MTEAATTVANAEPALRLRGVGRRFGGLQAVRDVDLDVPHGERHA
                     VLGPNGAGKTTLFNLVAGDLRVSDGSIELFGGDVTQAPARQRAKLGVARSYQQSRLFA
                     GLTVEENIYLAIVGVEGGRLLPVVLPKRNKRIRERAHEAAARVAIHDKLGELVDSLSH
                     GEHRQIAIAMALAARPKLLLLDEPASGLSRGERGLLADLLLGLGGEITLVLIEHDMDI
                     ALNVAEQVTMMHDGRVIARGTPAEIRANQMVHDLYLGRGHHDE"
BASE COUNT         1168 a         2807 c         2530 g         1261 t
ORIGIN      
        1 ggacacccgc cttcgccgct gcactcgatc gcgcactcga cgtcacggcc accgccgagc
       61 agtggatcag gcggcgcgga gatgtcgagc tcgtctcgcc tgctgcgctc ggcgtcgtct
      121 gcttccgccg ccgcgacggg cgggacgacg aggctatcgc ccgcttgaac cgggacgggc
      181 ttggtcacgt ctcctcgact cagatcgacg gatcgaccgt gagccgcttg tgcgtgaacg
      241 gctttcgcac ccggtggcga gacgccgagc aggtgctcga gaagttggcg ggcccgctgg
      301 actgacctac gcggccgcgc ccttgtcgaa ctcgtcgccc tcggcctcta tctgctccgc
      361 gtactcggct ccgatggcga tcaggtcgtc gatcctgatc cacgcgtctc cgagcgcacg
      421 ccctccggcg cgcaagaccg cgtcgcggaa cggaagcgcc cagcggtgct cgaagagtac
      481 gaccgcggca gacgtccctg gcgggatcat cgccccgatg tcgacgatgt cgccggtgct
      541 cgcctcgagc acctcgcggc cgagcgcctc ggctcccagc gcggcgccca cgtgcgcccc
      601 ctccgagccg gctgcaccga gaccgatgag gccgccgacc caggcgccga gctcgcgcat
      661 cccgtcctcg tcgaagtcgg tgctctgcag cgagatcagc tctccgtcgt cgcgcttggc
      721 gacgaagaga aggtcgatga cgcggatcag cccgctccgt cgcagcttcc gcagctcggc
      781 gcgcacctcc tcgcccggct gctcgccttc gaagccgatc gtcaccatct ggatcgggcc
      841 gatgctcatg agcgcacctc gccggcgacg gctcccgccc ggctgagccg ggctccgccg
      901 ccgcctgacc ctaggagcgt gcctttgttc gcggaaactg ccacatcctg tctcacgtag
      961 tacctccttc ttcgatgaag cgcgcgatcg ccgcggacgc ctggtcgaag tcgtcccaca
     1021 gaatgctgtg gccgccgggg agcgtggccg actccacatg cggtgcggcc ggcacgtcga
     1081 ccgagatcca ggagcgctcg ccgagcagca cgagcgtcgg gcagctcgcc cattcgggcg
     1141 gctcggtcga catctcgctg aacgcggtga tcaccgagca ctgcgagtag cgccagcgga
     1201 gccgcccgtc gtcgtgctgg acaagatgct gctcggcctc ctcgtcgagg aactcggcgg
     1261 gagtcgagta gtaggagccg gaggagagct tcgcctcgat cgcatcctcc gccgttgcga
     1321 aagacgcgtc ggggcgtgaa ccctccgcct gctcggcact gacggcaggc tcgacgtaca
     1381 gcgcgggatc gagcaggacg gcgcgctcga cgcgagccgg gaccgtatct gcgagctcga
     1441 cgacgaggcg cccgccgaag ctgtgaccga tccacgtcgc ggaatggacg ccgagcgcgt
     1501 ctgcggtctc gaccagatcg gcaacgtgcg tctcgatgtc ccagggcggt tcccagccgg
     1561 aaaatccgtg gccgcgcagg tcgactccca cgacgcgatg cctcgccagg gtggtctcgg
     1621 ccaggcgccg gaagcgccgg ccgtgcccgg tcacgccgtg caggcagacg accgcctgac
     1681 ccgcggcgtc tccccactcg taggtgttca ggagcacgtc aggatgatct tcccgaaatg
     1741 gtctcctgcg agccgcccct gtgccgcctc ggctcccgcg agagcgtgca ccgagtcgat
     1801 caccggacgc cacggcgcct cacccatcac gcggagaaga ccggcgaagt cgcgcgggct
     1861 gccgatcatc gtcccgagca gcgacagctg gttgaaatag acgggtcgca cctggagagt
     1921 ggcctcggtt ccaccggtcg cgccgaacac gacgacgcgg ccggccggcc ggcagcacgc
     1981 gagcgagtcg ggccaggtcg tgccgaccga gtcgacgacg agatcggcgc cgccgagctc
     2041 cttcacggcc gccacccagt ctccgtgggc gtagttcacc ccctcgtcgg cgccgagctc
     2101 gcgcgcccga tcgagcttct cgtccgagga cgaggtcacg atcacgcggg ccccttcgtg
     2161 caccgcgagc tggatcgcga acgtggagac accgcttccc gcaccgagta ccaacaccgt
     2221 ctcgccttct cgaagcccac cgcgcgtaaa cacggcgcga tacgccgtca gcgcggcgag
     2281 cgggaaagcg gcagcctcct cccacgagag gtgaccgggc ttcgggtagc agttctccgc
     2341 cggaaccgcg accagctcgg cataggtgcc gtcagtcggg cctccgagga cctcgaagtc
     2401 aggtccgccg acgtcctccc cgtcccccca gcgaagtgac ggcaggatca cgacctcgtc
     2461 gcccgtgtcc cggcgaatgc cggcgccgtc ggagccgggg acgagcggga gcgggtacgg
     2521 gtacacgccc ttcgagacga gcagatcgcg cctgttgagc ccggcgcacc gcagctcgac
     2581 gagcacctcg cccgcctcgg gctccggatc cgggatctcc tcgaaccgga gctcgccgcc
     2641 ctcgtgaacc cgaaccgcct gcatcagccc gtcctcaggg aggcccaggg ctcctcggtt
     2701 gacaggtcct cgagcccgta tccgtcgtct ccgatggcgg gcgagaggat cgcgatgatc
     2761 ttcgccggct gatcggtgtc gttgaacgtc gcatgcaccg cggagctcgg gatcagcgcg
     2821 gcgtcgcccg cggtgagcac gcgcttctcc tgttcgaccc actgctcgag catgccttcg
     2881 atcacgtaga tgacctcttc ctggccggga tggcggtgga agtcgtggca ttgcccgggc
     2941 agaaacgtcg cctcgatcgt gacgatctgg gagagaccgt cctcgggcga gctgaccgtc
     3001 gcgaagaccc cccaatccat ctcctgacgg tcgatctccg ccgccgactt gagcgcgccc
     3061 atcaggcgga caccgggatc gccttgaacc gtttcatgtt ctccgtcatc gccacctccg
     3121 tcggcagtcg ctccatcgag gaggcgccga agaacccgac gaccccttcc gtgcgctcga
     3181 gcacgtatgc agcatcgtcg ggctcggcga tcggaccgcc gtggcagagg acgatgatct
     3241 cggggttgac ccgcttcgcc acgtcgtgca tctcctggat cagcggcacg cagtcgtcga
     3301 gcgtcttcgc cgtctcggcg ccgatcgagc cgccggtagt gaggcccatg tgcggcacga
     3361 gcacgtctgc acccgcctcg gccatcgccc tcgcgtcgtc cgcgtcgaag acgtaagggg
     3421 aggtgagcag gtcgagctcg tgagcggcgc ggatcatgtc gacctcgtgg ccgtagccca
     3481 tgtcggtctc ttcgaggttc tggcgaaacg tcccatcgat caggccgacc gtggggaagt
     3541 tctgcacccc ggagaagccg atccgcttca gctcctcgag gaagacgggc atcagccgga
     3601 acgggtcggt tccgcagacc ccggcgagca ccggcgtctc gctcacgatc ggcagcacct
     3661 cggcggccat gtcgaccacg atcgcgttcg cgtcgccgta gggcatcagc cccgagaggg
     3721 agccgcgccc gcccatccgg tagcggcccg agttgtagat gatgatcagg tcggcgcctc
     3781 cgccctcggc gcactttgcc gagaggccgg ttcccgcccc cgccccgatg atggggcttc
     3841 cctgctcgat ctgcccgcgc agacgcgcga gcgcttgttg tctggtcacc tagctagccc
     3901 cctggatcag ctcgtggagc cggttcgcca tcgcgagcgc gaactcggga tcgttgacgt
     3961 cggcctcgat ctcgcgcacc tcgacgttct ccgtgtcgat tgtgtcgcgc acagcctgga
     4021 acagcacctc gtcggcctcc ggatcgtgga agacctggcc ctcgacggcg atcatcgaga
     4081 cgcccttcag cggcacgaag aggacggtcg ggccggtagc ggcgttcagc ttcgcggcga
     4141 tacgcccgcc gagctgcttg cactcctccg gcgtcgttcg catcagcgtg acggtcgggt
     4201 tgtgaacgta gaggttgcgg ctctcgaact tctcgggaac ggtgtcgcgg ggcccgaagt
     4261 tgaccatgtc gagggcaccg agcgagacga cctgcgggat gccggcggcc ccggcggact
     4321 cgagccgctc cggaacggcc gggaacacgc cgccgacgag ctcgtcgcag aactccgtcg
     4381 tcgtcacgtc gagagagccg gtgatgaagc cggccttgat cagctcctcc atcgagccgc
     4441 cgcccgcgcc ggtttggtga aacacgagca cctcgtagcc gagctcctcg agccgctccc
     4501 gcgccgtcgt cacggcaggc gtcgtcacgc cgaacatcga agcgacgatc agcggcttct
     4561 tctcgcccag gtcgggcacc gtccccttga ccgcccccgc gatcatgcct gcggcgttgg
     4621 tcatgatctt cgccgagatc tcgttgacgc cggcgatgtc gacgaccgaa tacatcatcg
     4681 tcacgtcgac cgagcccacg tacgggctcg tatcgcctga cgccaccgtc gagaccatca
     4741 gcttcggcac gccgatcggg agctggcgca tggcatgcgt cgcgagcgac gacccgcccg
     4801 agcctccgag cccgagagcg ccgtcgagcc gtccctcgtc gctgaggcgc ttcaggacga
     4861 ccgctgcgcc ttcggccatc gtcgctaccg ccgcaccccg gtcacccgcc tcggcgagcg
     4921 ccttgacgtc ggctcccacc gccgcggcga cctcctcgtt cccgatatcc ggggccacgg
     4981 tcggctcgaa gacgccgaca tcgacgagca cgacgtcgac gccgtgctcg cgaacgcgcc
     5041 cggcgagaaa ctcgtactcc ttgcccttcg tgtcgagcgt gccgacgagc accacggttg
     5101 gcacggcgtg ctcctttcag gtcgcgggcg agggccggga cctgtctaac acatccaccc
     5161 cggccgcgcg ggcggagctg gatcagccgt cgccgccgtc ctccgcgccg tccaaacccg
     5221 gccgccaggt ggggctggag tcgttgttgg cggcatcggt gatctcgacc tcgttgccgt
     5281 ccctgtcgac ggtgacgatc gcgccgcctc tggaaaacgc cagcagccga ccgtccggcg
     5341 accacgcggg ctcgaaggca tcctcggccg atcgaaccgc gcggcgaagc tccttgccgt
     5401 cgacgccgat cgtgtagatg tcgaacctca cgccaccgcg gttggcggcg aaggcgacgg
     5461 tcttgccgtc gggcgcccac gcaggtccat agctcttggc gtcgagcgtc gtcagctgcc
     5521 tgcgcaccga cccgtcgggc ttggcgatcc agatctcgat cgtgtcggtg ccaggcgttc
     5581 gtcgcgcgta ggcgatccac tccccgtcgg gcgaccaggc cggctcgatc tcgggcgcca
     5641 ggtcgtcggt caggcggcgc acatcgctgc cgtctgagtc catcacgaag atgtggcccc
     5701 cgccccgctg aaacgcgatc tgactcccgt ccggcgacca ggtggggcgt ccgtcttgtg
     5761 cgtcggagtt gctgatgttg cgggcgttgg ttccgtcggc gtccatgacg aagatgtcga
     5821 acgatccgtc ccgcgcgctg gcgaaggcga tcttcgtccc gtccggtgac cagtccgggt
     5881 cgatctggta cgcgagcccc tcggtcgtgg aggggtcgcc gcgatcctcg gtcacccgtg
     5941 cctcgccatt cccctcggcg tccacggcga aaagcgcgta gtcgccgtcg cgggtgctga
     6001 ccaccagcag gtcgcccgcg tccgcgctct tgccgcctcc accgcagccc gacgcgacga
     6061 gcgcgaacag gccgacgagc cccagaacga gaagccaccc ctttctcata gtgaaacgct
     6121 agccgtccga cgccttgtca gcggcgagcg gctcgacgcc gagatagcgt cgctgcagct
     6181 ccgagtcgcc cgcgagcttc gcggccgtgg tctcgtgcgc gatctgcccg gcgaccatga
     6241 tcaattggcg ctctgcgagt gccgtcgcga cgccgaggtt ctgctcgatc aggagaatcc
     6301 cgagaccctc ttgctcgagc tgctggaacg tctcgatcag gctctcgatg atcgccggcg
     6361 cgaggccctc ggacggctcg tccatgacga gcagcctggg gttcgtgacg agcgcccggc
     6421 cgatggccag catctgctgc tcgccgcccg acagctgcgc gccaccgttc cgtttgcgct
     6481 ctgccagcct cggaaagagg tcgtatacgc gtcggcgcgt ccattcctca ccctctccgt
     6541 tctccgctcc gcgtgcagcc atccgcagat gctcgtcgac ggtgagcgag ggaaacagcc
     6601 tacgaccctg cgggacgtag cccaggccct ggcgggcgat cttgtacgac tggccgccga
     6661 cgagctcgcg accttcgaaa cggatcgagc cgcgcgcatt aggcggggag atgcccatga
     6721 tcgcattgca cagcgtcgtc ttgcccatcc cgtttcggcc gacgatcgcg atcgactcgc
     6781 gacccatcgc gaacgtcacg ttctcgagcg cctgagcgct gccgtagaac gcactgagct
     6841 cctcgatctc gagaaccgcg cgtccagccg gctccgcggc cggctgcccg gcactcgcct
     6901 cggtcgtccc cgggtcactc atcgtggtgt ccccgtccga gatagaggtc gtgcaccatc
     6961 tggttggcac gtatctcggc gggtgtgccg cgcgcgatca cacgaccgtc gtgcatcatc
     7021 gtcacctgct ccgccacatt gagcgcgatg tccatgtcgt gctcgatcag gacgagggtg
     7081 atctcgccgc ccaggccgag cagcagatcg gcgagcagcc cgcgctcgcc gcgagagagc
     7141 ccggaagcgg gctcgtcgag cagcaggagc ttgggtctgg cggccagcgc catcgcgatc
     7201 gcaatctggc ggtgctcgcc gtgcgagagc gagtcgacga gctcacctag cttgtcgtga
     7261 atcgccacgc gggcggccgc ctcatgagca cgctcacgga ttctcttgtt gcgtttcggc
     7321 aggacgaccg gaagcaggcg gccaccctcg actcccacga tcgcgagata gatgttctcc
     7381 tcgacggtga gtccggcgaa gagcctcgac tgctggtacg accgtgcgac gccgagcttc
     7441 gcacgctgac gcgccggtgc ttgtgtgacg tcgcctccga acagctcgat cgagccgtcc
     7501 gagaccctca gatcgcctgc gacgaggttg aacaacgtcg tcttgcccgc tccgttcggg
     7561 ccgaggactg cgtgccgctc tccgtgcggg acgtcgaggt cgacgtcccg cacggcttgc
     7621 agcccgccga agcgccggcc gaccccccga aggcggaggg ccggctcggc gtttgcgacg
     7681 gtggtagctg cttctgtcac tcgccttact tgacgaccgt gttcgttacc cgaccgttca
     7741 cgacctcgcg gatcttgccc tgccac
//
LOCUS       DBNGNK010000196         4607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_678265,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000196 DBNGNK010000000
VERSION     DBNGNK010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_678265"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /gene="der"
                     /locus_tag="AC150V_05765"
     CDS             complement(<1..1103)
                     /gene="der"
                     /locus_tag="AC150V_05765"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR3762464.1"
                     /translation="MNANGEAPADDSIAGTVAIVGFPNVGKSTLANRISESRTAVVHE
                     TPGVTRDRKEILCDWAGKRFRLFDTGGVDEANRGPLGSAIVAQTREAIEAADVVVLVV
                     DARAGVTPGDEELATLLRAARTPVIVAANKIDDPGRDLEALEFHRLGLGDPVPISALH
                     GHGTGDLLDDVLARLPGAGEAPVGEEAIRVAVLGRTNVGKSSLLNALVGSERVIVSDV
                     PGTTRDAIDTVLERGETTLVLVDTAGLRRKRRQRQGIEYYSELRTLDAAKRADVALVL
                     VDSSEGIVEQDLTVADVARRAGCSTIVVLAKWDITELDIEEARGHLHRRLRQRPPVIA
                     VSARTGRGLERLLDLVEQVFAKHAGRVATAEFNR"
     gene            complement(1100..1753)
                     /locus_tag="AC150V_05770"
     CDS             complement(1100..1753)
                     /locus_tag="AC150V_05770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3762465.1"
                     /translation="MITPERSWAVGRVTIQPVVHLLSSLRVYGKDRVPRVGGCVIACN
                     HFSWADPPALGSACPRPIYYMAKIEAHRVPGLGELIRAFGTFSVRRGESDRDAVRLMR
                     QIVRDGNALGLFVEGTRQRRGVPGTVQPGAAMVALQEDVPIIPVAVHGSQAWRLGNFA
                     PISIAWGTPLRFEGIPKGGRGYKEASREVESEIHKLWAFLVDMHRLGRPRNATPPDR"
     gene            complement(1750..2379)
                     /gene="cmk"
                     /locus_tag="AC150V_05775"
     CDS             complement(1750..2379)
                     /gene="cmk"
                     /locus_tag="AC150V_05775"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR3762466.1"
                     /translation="MIVAIDGPAGSGKSTVARALAARLGFRYLDTGAMYRALTWLALR
                     DGVALDDGPALAALARAHPVSFGDEGSVVIAGEDVTGPIRRPEIDASVPVVARHAEVR
                     EVMRERQRALGNLGDSVIEGRDIGVVVAPHAEVKVWLIADASVRAARRHAEREGVGRG
                     ELADELRRRDERDAANTHRAPDAVEIDTTGLSVDGVLDRIADIVAERRA"
     gene            complement(2376..3665)
                     /gene="aroA"
                     /locus_tag="AC150V_05780"
     CDS             complement(2376..3665)
                     /gene="aroA"
                     /locus_tag="AC150V_05780"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR3762467.1"
                     /translation="MIVEPFARLDGHIAVPGDKSISHRAVLLAAVADGHTPISGFGRS
                     ADTEATIAAVRALGVEVEEPEVDALVVHGVGLRGLRAPDHVIDCGNAGTLVRLLAGLL
                     VGQERQDFELTGDESLRTRPMNRIAEPLRLMGAGIETADGRLPLRVRGRPLAGITYEL
                     PVASAQVKSAILLAGLYAAGETTVIEPAPTRDHTELMLAAAGVRIVTATNSVTVWPAP
                     QLALREIDVPGDFSSAAPFVVAATLVQGSVLHVHGVNLNPRRTGLLDVLERMGARIYV
                     YNRRRIGGEPAGDLEVVSAPLVAADVGPDEVPLAIDELPLYGLAASCARGESRLRGAE
                     ELRAKETDRIEAVVDGLRAIGAHINGTPDGFRVRGVPTRLRGGTIASRGDHRVAMLGA
                     VAGLASREGVQIEDAEAVAVSFPGFFELIDSLRSQTP"
     gene            complement(3662..>4607)
                     /locus_tag="AC150V_05785"
     CDS             complement(3662..>4607)
                     /locus_tag="AC150V_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR3762468.1"
                     /translation="APSLEASLADADLVVVAAPVAVLPDVVREVLVQAPETCCVTDVG
                     STKGAVCAVAAGDERFVGGHPICGAETRGPERATAELFEGATWFLTPDAAIAPERYRL
                     VHGFIASLGARPVAIDPAAHDRLVAVTSHLPHALANILLNQAGASRIEGHDPLQAAGG
                     SLRDMSRIAGANPRIWVDIFLDNREALASALAEHRRRVEQLESALAAGDAGFLARWIG
                     EASGNRRRMLATAYDDPGELQRLRVHVPDRPGVLAGIFQALGAERINVADFDLEHLSS
                     ERGGTLTILVAGEEEAVRANDLLEAQGYGVVVAPVLEA"
BASE COUNT          700 a         1652 c         1577 g          678 t
ORIGIN      
        1 cggttgaact cggccgtcgc gacccgcccc gcatgcttgg cgaagacctg ctcgacgagg
       61 tcgagaaggc gctcgaggcc gcgccccgtc ctggccgaca cggcgatcac ggggggacgt
      121 tgccgcaggc gccggtgcag gtgcccgcgg gcctcctcga tgtcgagttc ggtgatgtcc
      181 catttcgcca gcacgacgat cgtggagcag ccggctctcc tggccacgtc ggcgacggtc
      241 agatcctgct cgacgatgcc ctcggacgag tcgacgagga cgagcgcgac gtcggcgcgc
      301 ttcgcggcat cgagcgtccg cagctcggag tagtactcga ttccctggcg ctgccgtcgc
      361 ttgcgtcgca gccccgcggt gtcgacgaga acaagggtcg tctcacctcg ctcgaggacg
      421 gtgtcgatcg cgtcgcgcgt cgtaccaggg acgtcggaga cgatcacccg ctcgctgccg
      481 accagggcgt tcaggagcga ggacttgccg acgttggtcc tcccgagcac cgccacccgt
      541 atcgcctcct cgccgaccgg cgcctcccca gcacccggca atcgcgcgag gacgtcgtcc
      601 agcaggtcgc ccgtcccgtg tccgtggagc gccgagatcg gcaccggatc gccgagcccg
      661 agacggtgga actcgagcgc ctcgaggtcg cgcccggggt cgtcgatctt gttcgctgcg
      721 acgatcaccg gtgtccgcgc cgcccgcagc agggtggcga gctcttcatc cccgggagtg
      781 acgccggcgc gggcgtcgac caccaagacg acgacgtcgg cagcttcgat cgcctcccgg
      841 gtctgcgcga cgatcgcaga accgagcggc ccccggttgg cctcgtccac gccccctgtg
      901 tcgaacagcc ggaagcgttt tccggcccag tcgcacagga tctccttccg gtcgcgcgtg
      961 acgccggggg tctcgtgcac aacggctgtc cgcgactcgg agatgcgatt cgcaagagtc
     1021 gacttgccga cgttggggaa gccgacgatc gcaacggtgc cggcgatcga gtcgtcggcc
     1081 ggtgcctctc cgttcgcgtt caccggtcgg ggggtgtggc gttgcgtggg cgcccaagac
     1141 ggtgcatgtc gacaaggaac gcccagagct tgtggatctc gctctcgacc tcgcgcgagg
     1201 cctccttgta acccctaccg cccttcggga ttccttcgaa ccgcagcggc gttccccagg
     1261 cgatcgagat cggcgcaaaa ttgccgaggc gccaggcctg cgagccgtga acggccaccg
     1321 ggatgatcgg aacgtcctcc tgcagcgcca ccatcgccgc gcccggctgc acagtccccg
     1381 gaacgccacg gcgctgccgc gtcccctcca cgaagagccc gagcgcgttt ccgtcgcgca
     1441 cgatctggcg catcaacctg acagcatcgc gatccgactc gcctcgacgc acggaaaagg
     1501 tgccgaaggc acggatcagc tcgccgaggc cgggcactcg atgggcctcg atcttggcca
     1561 tgtagtaaat cggccgtggg cacgccgacc cgagcgccgg cggatcggcc cacgagaaat
     1621 ggttgcaggc gatgacgcag ccgccgaccc gcggaacgcg atccttcccg tagacccgca
     1681 gcgaggagag gaggtgcacg accggctgga tcgtgacccg gccgacggcc caggagcgct
     1741 ccggggtgat catgctcggc gctcggccac gatgtcggcg atgcggtcga gcacaccgtc
     1801 gaccgagagt cccgttgtgt cgatctccac agcgtcgggg gctcgatgcg tgttcgccgc
     1861 gtcacgctcg tcgcgccgtc gcagctcgtc cgccagctcc ccccgcccga ctccctcacg
     1921 ctctgcgtgg cgacgtgccg cgcgaacgga tgcgtctgcg atcagccaga ccttgacctc
     1981 cgcgtgcggc gcgacgacga ccccgatgtc gcgcccctcg atcacggaat ctcccaggtt
     2041 gcccagtgcg cgctggcgct cgcgcatcac ctcgcgcacc tccgcatgcc gggcgacgac
     2101 gggaaccgag gcgtcgatct cgggacgacg gatcgggccg gtgacgtcct cccccgctat
     2161 gacgaccgag ccctcgtcgc cgaagctgac aggatgagcg cgtgccaagg cggcgagcgc
     2221 cggaccgtcg tcgagcgcca caccgtcccg cagagcgagc cacgtgagcg cgcggtacat
     2281 cgccccggtg tcgagatagc ggaagccgag acgcgccgcc agcgcgcggg cgaccgtgct
     2341 cttcccggac ccggccgggc cgtcgattgc gacgatcatg gggtctgcga tctcagcgag
     2401 tcgatcagct cgaagaagcc ggggaagctt acagcgaccg cttctgcatc ctcgatctgg
     2461 actccctcgc gggacgcgag accggcgaca gctcccagca tcgcgacccg atggtcgccg
     2521 cggctcgcga tcgtgccgcc gcggagacgc gtggggacgc cgcggacccg gaagccgtcg
     2581 ggtgtgccgt tgatgtgagc accgatcgcc cgtagaccgt cgacgaccgc ctcgatccgg
     2641 tctgtctcct tggcccgcag ctcctcagcg ccccgcagcc tgctttcccc gcgtgcgcag
     2701 gatgcagcaa gtccgtacaa gggaagctcg tcgattgcga gcggcacttc gtccggaccg
     2761 acgtcggcag ccacgagcgg tgccgacacc acctcgagat cgccggccgg ctcgcccccg
     2821 atccgccggc ggttgtagac gtagattcgc gcccccatcc gctcgaggac gtcgagcagt
     2881 cctgtccggc gcgggttcag gttcacgccg tggacgtgga gcaccgatcc ctgtacgagg
     2941 gtggcggcga cgacgaaggg tgcggccgag gagaagtcgc ctggaacgtc gatctcgcgc
     3001 aaggcgagct gcggcgcggg ccaaacggtc acgctgttcg ttgctgtcac gatgcggaca
     3061 ccggcggcgg cgagcatcag ctctgtatgg tcgcgcgtcg gcgcaggctc gatcaccgtt
     3121 gtctctcccg ccgcgtacag cccggcgagc agaatcgcgg atttcacctg cgcgctcgca
     3181 acgggaagct cgtaggtgat gcctgccagc gggcgacctc gaacgcgtag cggaaggcgg
     3241 ccgtccgccg tctcaatccc tgcgcccatc agcctcagcg gctctgcaat gcgattcatc
     3301 gggcgcgttc tcagcgactc gtcgccggtc agctcgaagt cctgccgctc ctgcccgacc
     3361 aggaggcccg ccagcagacg caccagcgtc cctgcgttcc cgcagtcgat cacgtgatcg
     3421 ggcgcccgta gtccgcgcaa gccgacgccg tggacgacca gcgcgtcgac ctccggctcc
     3481 tcgacctcga caccgagggc gcgtaccgcc gcgatcgttg cctcggtgtc ggcggaccgg
     3541 ccgaagccgg agatcggggt gtgaccgtcg gcgacggcgg cgagcaggac ggctcggtgc
     3601 gagatcgact tgtcgccggg aacggcgata tgcccgtcga gccgtgcgaa cggctcgacg
     3661 atcacgcttc gagcaccggg gccacgacga cgccgtatcc ctgcgcttcg agcaggtcgt
     3721 tggcgcgcac cgcctcctct tcccccgcca cgaggatcgt gagggttccg ccgcgttcgc
     3781 tcgaaaggtg ctcgaggtcg aagtcggcga cgttgatccg ctcggctccg agagcctgga
     3841 agatcccggc gaggacaccc ggccggtcag gaacgtgcac gcgcaggcgc tgcagctcgc
     3901 cggggtcgtc gtacgcggtc gccagcatgc gtcgacggtt gccgctcgcc tcgccgatcc
     3961 agcgcgcgag gaatcccgca tcgccggcag caagagcgga ctcgagctgc tcgacccgtc
     4021 gtctgtgctc agccagagcc gatgcgagcg cctcccggtt gtcgaggaag atgtccaccc
     4081 agatacgcgg gttggcaccc gcgatgcggc tcatgtctcg caacgagccg cccgctgcct
     4141 gcagcggatc gtggccttcg atgcgcgacg cacctgcctg gttgagcagg atgttcgcga
     4201 gtgcgtgggg caggtggctc gtcaccgcga cgaggcggtc gtgggcggcg ggatcgatcg
     4261 ccacgggccg ggctccgagc gaggcgatga agccgtgcac gagccgatag cgctcgggtg
     4321 cgatcgcggc gtccggcgtc aagaaccagg tcgcgccctc gaacaactcc gcggtggccc
     4381 gctccggccc gcgggtctcg gcgccgcaga tcgggtgacc gccgacgaag cgctcgtcgc
     4441 ccgcggcgac tgcgcagacg gcacctttgg tcgagccgac gtcggtcacg cagcaggtct
     4501 ccggggcctg cacgagcacc tcccgcacga cgtcgggcag caccgccacg ggagcggcga
     4561 ccacgacgag atcggcatcc gccagcgacg cctcgagcga gggcgcg
//
LOCUS       DBNGNK010000197         3236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1324116,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000197 DBNGNK010000000
VERSION     DBNGNK010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3236
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1324116"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC150V_05790"
     CDS             complement(<1..417)
                     /locus_tag="AC150V_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762469.1"
                     /translation="MIAVGVAAWAVAFWALLPTAVPDDLRVPDIDVRSVFGAAAVVEA
                     ERYERLQLILWSLSQLVLLAVLAVYARWGTRYARESAGGRIATGMLLGMLGLGFAWLA
                     RLPFAGVSFWWARRHDLTEAGWGEWIFGDYFQLGGSF"
     gene            complement(438..1673)
                     /locus_tag="AC150V_05795"
     CDS             complement(438..1673)
                     /locus_tag="AC150V_05795"
                     /inference="COORDINATES: protein motif:HMM:NF025691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR3762470.1"
                     /translation="MESSPEEPRLRLVVADDLRRSRLTVLLRLLLALPHLIWLSLWAI
                     AVSVLALALWLAVLIDGRLPSVLHDFAASFVRYATHVGAYLFLIANPYPSFRGATTYP
                     VDVDIPPPQRQSRWTALFRLVLAFPALLLAGVLAGTFGGGSSTSYSGGVLGAVALLGW
                     FAALALGRMPRGLRDLGAYGLGYGAWAAAYVLLLTGRYPNARPSLVSPPQELPGHQVR
                     LALADEGTRSRAVVFFRPLLVLPHIVWIVLWGVAALGAIVAGWFAALAVGRLPTPLHR
                     FLAAYVRYWAHVTAFASVTGGPFPGFAGTPGAYTVDIEIASADRQHRLAVLARSVLAL
                     PAVFLALTLWGAVLVVALLGWFAALFTARMPRGLLELGAVSIRYQAQTAAYLSLVAGR
                     YPYAAPAVAAPVVAEEGTA"
     gene            1723..2667
                     /locus_tag="AC150V_05800"
     CDS             1723..2667
                     /locus_tag="AC150V_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR3762471.1"
                     /translation="MPTHSPPEREDVLRAAEVIRGRVHRTPMLSSRSLGPGRYLKAEL
                     LQRTGSFKPRGVLTKLTSLDVRERARGVISVSAGNHAQALAWGAAVEGIDALVVMWQG
                     ATESKVAAARAYGAAVDLGASGPGDAFDRLEELQEETGRVFVHPFDDPLVVAGQGTVG
                     LEILEDVPSADAVVVPVGGGGLVTGIAAACAPLGVRVIAVEPENSPALHEGLRAGSPV
                     AVEARSVADGLNAPFAGELAIQVCASLGVESVLVSEAEIEEAMRFLYGRAKLACEPAG
                     AAAVAALLNGTVAEKTVVAVVSGGNVDPRRAAAILAER"
     gene            2664..2879
                     /gene="rpmE"
                     /locus_tag="AC150V_05805"
     CDS             2664..2879
                     /gene="rpmE"
                     /locus_tag="AC150V_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR3762472.1"
                     /translation="MKADIHPEYVTAHVTCSCGNEFTTRSTKPDLHVEICSACHPFYT
                     GKQKLVDSGGRVERFQRRLEKAQASRG"
     gene            2907..>3236
                     /locus_tag="AC150V_05810"
     CDS             2907..>3236
                     /locus_tag="AC150V_05810"
                     /inference="COORDINATES: protein motif:HMM:NF018798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR3762473.1"
                     /translation="MSVSYGGQAVLEGVMMRGPSCWTVAVRKPNGEIAEVTNGITSPM
                     QRRRVWRLPVIRGVVALGESLAIGFRALAISANYAAQDPNSDEEVKTEISRGMIIFSF
                     AIAIGFAL"
BASE COUNT          557 a         1092 c         1193 g          394 t
ORIGIN      
        1 gaagctgcct ccgagctgga agtagtcacc gaaaatccac tccccccatc ccgcctcggt
       61 gagatcgtgg cgccgtgccc accagaacga gaccccggcg aacggcagac gcgcgagcca
      121 ggcgaagccg agcccgagca tcccgagcag catgccggtc gcgattctcc cgcctgccga
      181 ttcccgcgcg taacgcgtcc cccagcgcgc gtagacggcg agaacggcga gcagcacgag
      241 ctgtgagagc gaccagagga tcagctgcag ccgctcgtag cgctcggctt cgacgacggc
      301 cgcggccccg aacacggagc ggacgtcgat atccggcacg cggaggtcgt cggggacggc
      361 ggtcggcaga agcgcccaga aggcgacggc ccacgccgcg actccgacag cgatcacgga
      421 tatccgaagt ctgccgctca ggccgttccc tcctccgcga cgacgggtgc cgccacggcc
      481 ggcgctgcgt acgggtagcg cccggcgacg agcgagaggt aggcggcggt ctgagcctgg
      541 tagcggatcg agaccgcgcc gagctcgagc aagccgcgcg gcatccgagc cgtgaacagc
      601 gcggcgaacc aaccgagcag cgccacgacc aggacagctc cccagagcgt gagcgcgagg
      661 aagaccgcgg ggagagcgag caccgatcgg gcgagcaccg cgagccgatg ctgacggtcg
      721 gctgacgcga tctcgatatc gacggtgtac gcgcccggcg tcccggcgaa gccgggaaac
      781 ggccctcccg tgaccgacgc gaaggccgtg acgtgagccc agtagcgcac gtaggcggcc
      841 aggaagcggt gcagcggggt cgggagccga ccgacggcca gggctgcgaa ccagccggcc
      901 acgatcgcgc cgagagccgc aaccccccag agcacgatcc agacgatgtg cgggaggacg
      961 agcagcgggc ggaagaagac gacggcacgt gaccgcgtgc cctcgtccgc gagagcgagg
     1021 cgaacctggt gtcctgggag ctcctggggc ggtgacacga gcgaggggcg cgcattcggg
     1081 tagcggcccg tgagcaggag gacgtacgcc gccgcccagg ccccatagcc gagcccgtac
     1141 gcgccgaggt cgcggagtcc gcgcggcatt cggccgagag cgagggcggc gaaccaaccg
     1201 aggagcgcga cggcgccgag aacgccgccc gagtacgacg tcgacgaccc gccgccgaac
     1261 gtgcccgcga ggacgccggc cagcagaagg gcgggaaacg cgaggacgag gcgaaacaga
     1321 gcggtccagc ggctctggcg ctgtggtggc gggatgtcga catcgaccgg gtaggtcgtc
     1381 gcgccccgga agctggggta gggattggcg atcaggaaga ggtacgcgcc gacgtgtgtc
     1441 gcgtagcgca cgaaagaggc ggcgaaatcg tggaggacgc tcgggagccg gccgtcgatc
     1501 aagaccgcca gccagagagc gagggcgagt accgagacgg cgatcgccca gaggctcagc
     1561 cagatgagat ggggcagggc gagcaggagc cggaggagga cggtcagtcg cgaccgccgc
     1621 aggtcgtccg ccacgaccag ccggagtcgt ggctcctcgg gcgaggactc cacgccacga
     1681 ttctgcccgg acgcctggac gggacgacta cgatcgcaca ggatgcccac acactccccg
     1741 ccggagcgcg aggacgtcct ccgggccgcc gaagtgatcc gcgggcgcgt ccaccggacg
     1801 ccgatgctct cctcccgcag cctcggcccc ggccgctact tgaaggccga gctgctgcag
     1861 cgcaccgggt cgttcaagcc gcgcggagtg ctgaccaagc tcacgtcgct cgacgtgcgc
     1921 gagcgcgccc gcggcgtcat cagcgtctcg gccggaaacc acgcccaggc gctcgcctgg
     1981 ggcgcggcgg tcgaagggat cgacgcgctc gtcgtgatgt ggcagggggc gaccgagtcg
     2041 aaggtcgccg cagcgcgcgc gtacggtgcc gccgtcgatc tcggtgcctc ggggccgggc
     2101 gacgcgttcg accggctcga agagctacag gaagagacgg ggcgggtgtt cgtgcatccc
     2161 ttcgacgacc cactcgtcgt ggcggggcag ggcaccgtcg gactcgagat cctggaagac
     2221 gttccgagcg ccgacgccgt tgtcgtccct gtcggcggcg gcgggctcgt gaccgggatc
     2281 gccgcggcct gcgctccgct gggggtgcgg gtcattgccg tcgagcccga gaactcgccc
     2341 gccctgcacg agggactgcg agcgggcagt cctgtggctg tcgaggctcg ctcggtcgcc
     2401 gacgggctca acgcgccgtt cgccggcgag ctcgcgatcc aggtctgcgc ctcgctaggg
     2461 gtcgagagcg ttctcgtctc ggaggcggag atcgaagagg cgatgcgatt cctctacggc
     2521 cgcgcgaagc tcgcctgcga gccggccgga gcggcggcgg tcgcggcgct cctgaacggc
     2581 accgtggcgg aaaaaacggt cgttgccgtc gtctcgggag gcaacgtcga cccccgtagg
     2641 gctgctgcta ttctggctga gcgatgaagg ccgacatcca tcccgagtac gtgaccgcgc
     2701 acgtcacgtg ctcatgcggc aacgagttca cgacgcgctc gacgaagccg gatctccatg
     2761 tcgagatctg ctcggcctgc caccccttct acaccggaaa gcagaagctg gtcgacagcg
     2821 gcggccgcgt cgagcggttc cagcggaggc tggagaaggc gcaagccagc cgcggctagc
     2881 ggcatccttc tacctgtgag gtgcccgtga gcgtcagcta cggcggccag gcggtgctcg
     2941 agggcgtgat gatgcgcggc ccctcgtgct ggaccgtcgc cgtgcgtaag ccgaacggcg
     3001 agatcgccga ggtcacgaac gggatcacct cgccgatgca gcgtcggcgg gtctggcgcc
     3061 tgccggtgat ccgcggcgtt gtcgccctcg gcgagtcgct cgcgatcgga ttccgcgcgc
     3121 ttgccatctc ggccaactac gcggcgcagg atcccaacag cgacgaggag gtcaagaccg
     3181 agatctcgcg cgggatgatc atcttctcct tcgcgatcgc catcggcttc gccctg
//
LOCUS       DBNGNK010000198         2835 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1111493,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000198 DBNGNK010000000
VERSION     DBNGNK010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1111493"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC150V_05815"
     CDS             complement(<1..362)
                     /locus_tag="AC150V_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014659.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR3762474.1"
                     /translation="MDSLRGQLLVAGPRLIDPNFRRTVVLIGEHSDEGAMGVVLNRPS
                     TVSVEEAAPPLSGLDGLDAAAEPIYVGGPVQPEAVVVVGEFEAPEQAPVLIFGSIGFL
                     PAESDPIELGDMVRSRVFA"
     gene            423..1433
                     /locus_tag="AC150V_05820"
     CDS             423..1433
                     /locus_tag="AC150V_05820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016816934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HIR3762475.1"
                     /translation="MGEVNREVRLVARPQGVPAEDLFEIVEGTVPEPAEGQFLVRNAF
                     LSVDPYMRPRMNDMRSYVSPFTLGETMTGGAVGKVVASRHEGFPEGAWVVHMLGWREW
                     ALSDGHGVLPVDRSYGPVATALGVLGMPGFTAWYGLLELGEPKEGETVWVSAAAGAVG
                     SAAGQIAKLRGCRVIGSAGSPEKLTWLHELGFDHAFGYRNGSLRDALREAAPDGIDLY
                     FDNVGGDHLEAAIGALRTHGRIVACGSISRYNEEEPTPGPSNLFMIVTKRLTVRGFII
                     SDHWDRFPEFLAEMGPLVRDGKVRHHETIVDGIENAPRAFLGMFEGANIGKMLVRVGP
                     DE"
     gene            1548..1754
                     /locus_tag="AC150V_05825"
     CDS             1548..1754
                     /locus_tag="AC150V_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003166433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR3762476.1"
                     /translation="MSVARVTEITATSTKSFEDATRVGLKRATKTLRNVKSAWIKEQT
                     VRVDDTGKISEYQVNMLVTFILED"
     gene            complement(1911..2606)
                     /gene="trpA"
                     /locus_tag="AC150V_05830"
     CDS             complement(1911..2606)
                     /gene="trpA"
                     /locus_tag="AC150V_05830"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR3762477.1"
                     /translation="MPKTLVIYLMSGRETPALAEAAVEGGADIVELGFPFSDPLADGP
                     VIRAAGERALAAGMGTKACLECLAETRSRVGATPLVPMTYSALLEAYGWERFEQDARA
                     AGASGMIIADLPADERPEIRRIQLVAPTSTDERIALAAERTDGWLYLVTLTGTTGARS
                     ELSAELATLAERSRAVTDVPLYAGFGIATPAHASAAASVTDGVVVGSRAVEVAGDGPA
                     ALRAFVASLRAAL"
     gene            complement(2606..>2835)
                     /locus_tag="AC150V_05835"
     CDS             complement(2606..>2835)
                     /locus_tag="AC150V_05835"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630680.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR3762478.1"
                     /translation="ARYLPCTDDEALEAFARLARTEGIIPALEPAHALARIDELDAEL
                     IAVCLSGRGDKDLAEALRGLAERGLSPQSDA"
BASE COUNT          448 a          951 c         1020 g          416 t
ORIGIN      
        1 gcgaagaccc gggagcgcac catgtcgccg agctcgatcg ggtcactctc ggcgggtagg
       61 aagccgatcg agccgaagat gaggacgggt gcctgctcag gcgcctcgaa ttcgcccacg
      121 acgacgaccg cctcgggttg cacggggccg ccgacgtaga ttggctcggc ggccgcgtcc
      181 aggccatcga gccccgaaag cggcggtgcg gcctcctcga cggagacggt cgacggtcgg
      241 ttgagcacga cccccatcgc gccctcgtcg ctgtgctcgc cgatgaggac gacggtgcgg
      301 cggaagttcg gatcgatgag tctcggcccg gctaccagca gctgtccgcg cagggagtcc
      361 atttcggtgc acgatacgca gctcacgcac cgcgcgtacc gcgggggaca taggatcgtc
      421 gtatgggtga ggtcaaccgc gaggtgcggc tcgttgcccg gcctcagggc gtccctgccg
      481 aggatctctt cgagatcgtc gagggcaccg ttccggagcc ggccgaggga cagttcctcg
      541 tgcgcaacgc cttcctctcc gtcgatccgt acatgcgccc gcgtatgaac gacatgcgct
      601 cgtacgtctc cccgttcacg ctcggcgaga cgatgaccgg cggtgcggtc ggcaaggtgg
      661 tcgcgtcgcg gcacgagggc tttccggagg gagcctgggt cgtccacatg ctcggctggc
      721 gcgagtgggc tctctcggac ggtcacggcg tgctcccggt cgatcgttcg tacgggccgg
      781 tcgccaccgc gctcggcgtc ctcggaatgc ccgggttcac ggcgtggtac ggcctgctcg
      841 agctcggcga gccgaaagag ggcgagaccg tgtgggtctc ggccgccgcc ggggcggtcg
      901 ggagcgccgc cggccagata gccaagctgc gcgggtgccg cgtgatcggc agcgccggct
      961 ccccggagaa gctgacatgg ctgcacgagc tcggcttcga ccacgcgttc ggctaccgga
     1021 acggctcgct gcgcgatgcg ctccgcgagg cggcgcccga cggcatcgac ctctatttcg
     1081 acaacgtggg aggcgaccac ttggaggcgg ccatcggcgc actgcggacg cacggccgga
     1141 tcgtggcctg cggatcgatc tcccgctaca acgaagaaga gccgacgccg ggtccgagca
     1201 atctgttcat gatcgtcacc aagcgcctca ccgttcgcgg cttcatcatc agcgatcact
     1261 gggatcggtt cccggagttc ctcgccgaga tgggtccgct cgtgcgtgac ggcaaggtcc
     1321 gccaccacga gacgatcgtg gacggaatcg agaatgcgcc gcgtgctttc ctcggcatgt
     1381 tcgagggcgc caacatcggc aagatgctcg ttcgcgtcgg ccccgacgag tgacatccgt
     1441 cgcggccgga gccggaacgt cgagcgcacg ccacacgcgc cgttcgcgta agccgcacgt
     1501 ccgccacacg aatagagttg cccggcaagc gacaaggagg cgtaaccatg tccgtcgcca
     1561 gagtgaccga gatcacagcg acctcgacga agagcttcga ggatgcgacg agggtagggc
     1621 tgaagcgcgc gacgaagacc ctccgtaacg tcaagagcgc gtggatcaaa gagcagaccg
     1681 tgcgcgtcga cgacacgggc aagatctccg agtaccaggt gaacatgctc gtcaccttca
     1741 tcctggagga ctgagcttcg cgggcgcggt cgccggcccg atcggcggtg agcacgggtc
     1801 gccagaccaa ctagggaatc ggccgctcag gatccgaagg gcagctctgc tctccgggcg
     1861 ggtcggggtt tctcagcccc ggcccgtctc cacacgacgt tgggatggcc tcagagagcc
     1921 gcgcgcaggc tggcgacgaa tgcacgcagt gcggccgggc cgtccccggc cacctcgacg
     1981 gcacgggagc cgaccacgac gccgtcggtg acggaagctg cggcggacgc gtgagcgggc
     2041 gtcgcgatgc cgaagccggc gtagagcggc acgtcggtga ccgcacggct cctctcggcg
     2101 agcgtcgcga gctccgcgga cagctcgctg cgggcgccgg tcgtgcccgt cagggtgacg
     2161 agatagagcc agccgtcggt tcgctccgcg gccagcgcga ttctctcgtc ggtggaggtc
     2221 ggagccacga gctggatccg ccggatctcc ggccgctcgt cggcgggaag gtcggcgatg
     2281 atcatcccgg acgcgccggc ggcgcgcgcg tcttgctcga atcgctccca gccgtaggct
     2341 tcgagcagcg cggagtaggt catcgggacg agtggggttg cgccgactcg actccgggtc
     2401 tcggcgaggc actcgaggca cgccttcgtg cccatgccgg ccgcgagcgc gcgctcgccg
     2461 gccgccctga tcacgggccc gtcggcgagc ggatcggaga acgggaagcc gagctcgacg
     2521 atgtcggcac cgccctcgac ggccgcctcg gccagcgccg gtgtctcgcg ccccgacatc
     2581 aggtagatga cgagcgtttt cggcatcagg cgtccgactg cggggacagt ccacgctcgg
     2641 cgaggccccg cagcgcctcg gcgagatcct tgtcgccgcg acccgacagg cagacggcga
     2701 tcaactccgc gtcgagctcg tctatacgag cgagggcgtg cgcaggctcg agcgccggga
     2761 tgatgccctc ggtgcgggcg aggcgagcga acgcctcgag cgcctcgtcg tcggtgcagg
     2821 ggaggtagcg cgcgc
//
LOCUS       DBNGNK010000199        10409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_537000,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000199 DBNGNK010000000
VERSION     DBNGNK010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_537000"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC150V_05840"
     CDS             complement(<1..235)
                     /locus_tag="AC150V_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976461.1"
                     /note="catalyzes condensation of pyruvate and acetyl-CoA
                     to form (R)-citramalate; functions in isoleucine
                     synthesis; belongs to the alpha-IPM synthetase/homocitrate
                     synthase family; it is difficult distinguishing these
                     proteins from enzymes in that family; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3762479.1"
                     /translation="MTLDPDRIWTGEVNRMALDPQPHVTVGLYDTTLRDGEQTVGVVL
                     TPEDKLEIARALSDAGIDRIEAGFPRVSDDDWRA"
     gene            complement(279..1436)
                     /locus_tag="AC150V_05845"
     CDS             complement(279..1436)
                     /locus_tag="AC150V_05845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3762480.1"
                     /translation="MTWPRDEAKLARVRSLMAEREIDALVVRAPDNVLYLTNFWGMKG
                     YDAAVFPREGDPILICLEPSAEDAARTAWTDEVLLFDGYDPRDPRPPGLRALDLARSL
                     STGFGTVGVELSLGTQAADRMVGEPTTFAKAWFDAFPEAEDATPLLNEARAIKSEQEL
                     ARMRLANEIAAAAMEHVQGVLRPGMTEAQAAAEWLGFVHGEGTGWHGTVDLALGFALV
                     WSGPGIRTFTATTNRPVVEGEPTLFEIWVCADGYWCDHTKNLVPGELKPEYRELEASL
                     RAVYDDAVAFARDGSSLAELDRRVRVAIAEAGYPGQPSHPICHGVGARAHEPPYAHQA
                     GGGEIRAGMVLAIEPGCYWADGGGLRFEDNFLVTDADPEKLCPFPDGIVRV"
     gene            complement(1581..2453)
                     /locus_tag="AC150V_05850"
     CDS             complement(1581..2453)
                     /locus_tag="AC150V_05850"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005476110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3762481.1"
                     /translation="MKLVTFGEGDGRVGFLDGDEIAVLDVPTMREYFERGGADESGER
                     VALAETRLRAPIAPKKFFHTAGNFREHEEESKRVDWDHEIAPWIVFFQNVDAIIGPDE
                     PVVYPEHLTEELDYELELAVIIGRGGKWFSPEEAMDYVAGYVIFNDITARDIQRREMR
                     SGVFSFCKAIDTFCPLGPWIVTPDEIPDPHDLSMELRVNGEPRQESHSGNMSVTIPEI
                     VSHYSPLGYSAGDVLSTGTVSGVAGFSEDAASLYLRPGDVMECEIERVGVLRNPVVSW
                     QEGHGEPPQERVRW"
     gene            complement(2450..3682)
                     /locus_tag="AC150V_05855"
     CDS             complement(2450..3682)
                     /locus_tag="AC150V_05855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3762482.1"
                     /translation="MAAVTIDRERLVDWASRAIGVPSFTGDELAMARLMAETYRELGL
                     RVQWQQVEDGRANAIGIWEGTGGGSSLMFNGHMDTSYSGNEPWLAGVKGFQPEAFVRD
                     GRLYGLGISNMKGALACYVEAVRALQDAGVRLRGDVMIAAVCGEIEKTQQGDAIGAQY
                     RGYAAGSRYLVTHGGLADMCILGEPTEGKVVLGHFGSIWLRISTQGNFIHTAFSEGKR
                     ELNSILRMREVLDAVLDWIPTWEDDPENAHRGAKAIVNVGAVQGGFGWRVSRTPHRTD
                     LFLDVRVPPTKPMPVARQQVLDMVRGLASQFPDYGVEGEVYVTAPGAEIDEGHPLITA
                     IDASHTEVFGSPPERDVTRWFSDASALTRYGVPTVNYGTSTGLLDTTYGENLDIEGLV
                     KMAHVYALAAMRACGVAA"
     gene            complement(3694..4770)
                     /locus_tag="AC150V_05860"
     CDS             complement(3694..4770)
                     /locus_tag="AC150V_05860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3762483.1"
                     /translation="MTGQVDNGLGVYLQALPVDVMLDFAGHAEQAGFKNVWLSEITFG
                     DAFVPAAAIACRSTRIGIATGIVGLWARSPVTTAMTAASLHRLSSGRFVLGVGLQSQT
                     YVDNWHGREYERPLRAVREYLTMMRRLVDGELTTYEGEIFRVKGFQLMMPPSDMRLPL
                     YLAGIGPKMAQLAGELADGILGYFYSVEYLRDVVMPNLRIGAERAGRSLDGFDVAVGF
                     PSVVTPDMRGIEQAKGQVVMFTTATKSSVSYQTSVTVAGFEAEMNDIEALVAAADVDA
                     AVALVPDEMADALTLSGSPEHVWERVAAYRAVGATTVVLNPSAPNAWFPLYEGHFPDG
                     ASPPDFDFPAYLEVIGTTIGGLGL"
     gene            complement(4775..5851)
                     /locus_tag="AC150V_05865"
     CDS             complement(4775..5851)
                     /locus_tag="AC150V_05865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3762484.1"
                     /translation="MGAPFRIGVMQLTMEPLDEMLETARVMDEAGMDTVWLAEAYPWW
                     RKHQMEARSSTVVSALMARETERLTIGWGIISPYTRHPVQAAMDARVVQEAAGPDRFI
                     VGFGTSKIFLNNTKQDGPPKVTKPLAHMRDSVEIVRGVLSGQRFEYEGRVFGADVPAL
                     SDDAHSPRGEVPVYVAGTAPLMQQLGGEIGDGLLTPSITTPAFVRYTRENVETGAAVS
                     GRDPDEVDIGCTIVASIGEDREAGRDGAREIAGMYLANKVQNIRGSADTLLELAEIDP
                     EEIRPVAEAMERGGRLAAKAEVTDSILDRCKPIAGTPADCIEAIEEYRQAGCTHVMLE
                     LWGGDRHGQIRLFGEKVLPHFRSG"
     gene            complement(5958..6719)
                     /locus_tag="AC150V_05870"
     CDS             complement(5958..6719)
                     /locus_tag="AC150V_05870"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020066179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR3762485.1"
                     /translation="MSSAPHWLASRAVLVTGAGRGIGQGVAVHLASLGADLALVQRGD
                     AIETRREIEALGCRAVVLQADLADASAAEEAVDAAADALGRLDAVVCNAGTIERHPAL
                     DHPLDAWRRTIDLNLTSVFVTARAAARRFVAQQTGGSIVTMASVLSFQGGVNVAAYAA
                     SKGGVAVLTKALANEWAPLGIRVNAVAPGYVESELTEPLRADPQRAAEIHSHIPAGRW
                     GRPEDVAGAVAFLLSPEAEYVHGHVLAVDGGWLAR"
     gene            complement(6716..7165)
                     /locus_tag="AC150V_05875"
     CDS             complement(6716..7165)
                     /locus_tag="AC150V_05875"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3762486.1"
                     /translation="MRIEGTKDFEAPREVVWSVINDPEQMAGLMPGIESFEIVDDRHW
                     TAKVKVPLGLGGLKMTITFEKVEERPLEYASLNAKGTGVGAMMNMTTQFTLSGEGDAT
                     TMDWEADVKIAGPVGSMGQRVLQPIVNQQVSNVLTALEKQVAEQTSA"
     gene            complement(7162..9609)
                     /locus_tag="AC150V_05880"
     CDS             complement(7162..9609)
                     /locus_tag="AC150V_05880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017240873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3762487.1"
                     /translation="MTTTLIEQQTTLEKGFVGQNVPRKEDRRLVQGQGVFVDDIKRHN
                     TGFVHFVRSPYAHARIVSIDVSKALELQGVFGTLTGDEVAILTDPFFQIASPPGANVK
                     DYALAVGKARYVGEPIVAVVAASRELARDASELVEVEYEPLDALVDARRALDDGAPVL
                     HDEADGNLSWEGTYEWGDLESAKAEADHVIEIEQLHFDRFNSTPLETDGALVEYNRGT
                     GQWTLYTNNQFPGFAVIMMAPALRVGIDKLRVVTQDIGGGFGNKITSHPQLVACCLLA
                     RKLNRPIQWTEWRTEFHLSMSHGNERWFQDIEVYAKSDGTLLGFSCKALDDAGAYLRY
                     EPLGGVIWSQVTPGMYRWRNIKLEFTQVMTNKAPVSPNRGYSRMQHLWFCERLVDIVG
                     AELGIDPVEMRKKNYVRAEEMPYETPNGCVYDSGDYARCLDIALELVGYDTIDERRED
                     AKRRGKLLGIGIGSTLDSGTNNFGQSQLINPELQFSGNNEVATVKLDIFGEVVVTLGT
                     TPQGQGHETTASQVVADILGCDVDAVTVRVGHDSYWNSHAGFSGTYASQFAVTGLGAV
                     KGAAEELAGDMRLLAAMVLGADGPDDIELADGFARRKDNPEAALPFMALGAIPNANNA
                     GFPEDPPTMNRRFVYRPPFQVPDKEKKFGNLTLTYATQVHACVVEVDPETGYYELVDY
                     AAVDDCGTRINPRIVEGQVMGATAHAIGAAIWETFAYDEEGNLLTPNFYDYHVPHALD
                     MPPLKTSAIESPSPFTPLGAKGMGEGGGGGIHCICSALQDALRSGGHEVYVFDSHNPY
                     HRVWEMLQGETSAKVSR"
     gene            complement(9606..10088)
                     /locus_tag="AC150V_05885"
     CDS             complement(9606..10088)
                     /locus_tag="AC150V_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3762488.1"
                     /translation="MAETPSLSRTISVEVNGDLYEREVESRRLLVHFIRDDIDLTGTH
                     IGCDTGNCGACTVHLDGLPVKSCMVLAVQAEGHSVRTVEGLADGDELTPLQQAFADHH
                     GLQCGYCTPGMLMSATALLAENPSPDEAEVRKALQGNLCRCTGYWNIIEAVVKAGGGS
                     "
     gene            complement(10091..>10409)
                     /locus_tag="AC150V_05890"
     CDS             complement(10091..>10409)
                     /locus_tag="AC150V_05890"
                     /inference="COORDINATES: protein motif:HMM:NF015415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HIR3762489.1"
                     /translation="TGDGFEGVSLGRDGTYVANAAASVSNGTVRVALGCVDAVPVRAT
                     AMEEALAGGDLSPASVEAAAAGLGATLDPPSDVHASAEYRRRLAEVSAVRAVLSAADR
                     RKG"
BASE COUNT         1608 a         3670 c         3378 g         1753 t
ORIGIN      
        1 ccgctcgcca gtcgtcatcc gagacccggg ggaagccggc ttcgatccga tcgatgcccg
       61 cgtcgctgag tgcccgcgcg atctcgagct tgtcctcggg tgtcaagacg acgccgactg
      121 tctgctcgcc gtcacggaga gtcgtgtcgt acaggccgac ggtgacgtgc ggctgcggat
      181 cgagcgccat ccggttgacc tcgccggtcc agatgcgatc ggggtcgagc gtcacgccct
      241 cgactccggc gcagcgaaca ggttctcacg ggctcgcctc acacgcgcac tatcccatcc
      301 gggaaggggc agagcttctc cgggtcggcg tcggtcacga ggaagttgtc ctcgaagcgg
      361 agcccgccgc cgtcggccca gtagcaaccg ggctcgatgg cgagcaccat gcccgcgcgg
      421 atctcgccgc cgccggcctg gtgggcgtac ggcggctcgt gcgctcgggc gcccacccca
      481 tgacagatcg ggtgggacgg ctggcccgga tagcccgcct cggcgatggc gacccggacg
      541 cgacgatcga gttccgcgag cgacgagccg tcgcgcgcga aggccaccgc gtcgtcgtac
      601 acggcccgta gagacgcctc cagctcccgg tactcgggct tcagctctcc tggcacgagg
      661 ttcttggtgt ggtcgcacca gtagccgtcg gcgcagaccc agatctcgaa caacgtcggc
      721 tcgccctcga cgacggggcg gttcgtcgtc gcggtgaagg tgcggatccc gggacccgac
      781 cagaccagcg cgaatcccag cgcgaggtcg accgtgccat gccagcccgt cccctccccg
      841 tggacgaagc ccagccactc ggcggccgcc tgagcctcgg tcatccctgg ccgaaggact
      901 ccctggacat gctccatcgc cgccgccgcg atctcgttgg cgaggcgcat ccgtgccagc
      961 tcctgctcgc tcttgatcgc acgcgcctcg ttgagcagcg gcgtcgcgtc ctcggcctcc
     1021 ggaaacgcat cgaaccacgc tttggcgaag gtggtcggct cgccgaccat gcggtcggcg
     1081 gcctgagtcc cgagcgacag ctcgacgccc accgtgccga acccggtgct cagggagcgc
     1141 gcaaggtcga gcgcgcgcag gccggggggt cgtgggtcgc gtgggtcgta cccgtcgaag
     1201 agaagtacct cgtccgtcca ggccgtgcgc gcggcatctt ccgcggacgg ctccagacag
     1261 atgaggatcg gatcgccctc acgtggaaag accgcggcgt cgtacccctt catcccccag
     1321 aagttggtca ggtagaggac gttgtccggc gcgcggacga cgagcgcatc gatctcgcgc
     1381 tccgccatca gcgagcggac gcgggccagt ttggcctcgt cacgaggcca cgtcacaggc
     1441 ccctggcccc tgtcgacgaa tcgtcacaca ctcgttgcac tcgacacggc cgctgcgggc
     1501 aggaccaggg gactgtcccc cggacatggc ccgttcggcc ggatgaggaa ggattggcac
     1561 gatttcggca cactcgtccg ctaccagcgc acgcgctcct gcggcggctc gccgtggccc
     1621 tcctgccacg agacgacggg gttgcggagg acgccgactc gctcgatctc gcactccatg
     1681 acgtcgcccg gccgtagata gagggacgct gcgtcctcgg agaagccggc gacgcccgag
     1741 acggtgccgg tcgacagaac gtcgccggcg gagtatccga gcggcgaata gtgactcacg
     1801 atctccggaa tcgtcaccga catgttcccc gaatgggact cttggcgcgg ctccccgttg
     1861 acgcggagct ccatgctcag gtcgtgcggg tcgggtatct cgtcgggcgt cacgatccac
     1921 ggcccgagcg ggcagaacgt atcgattgcc ttgcagaacg agaacacacc tgaccgcatc
     1981 tcgcggcgct ggatgtcgcg ggccgtgatg tcgttgaaga tcacgtagcc ggctacgtag
     2041 tccatcgcct cctcaggcga gaaccacttt ccgccccggc cgatgatcac ggcgagctcg
     2101 agctcgtagt ccaactcctc ggtcaggtgc tccgggtaca cgactggctc gtccggtccg
     2161 atgatcgcgt cgacgttctg gaagaagacg atccagggag cgatctcgtg atcccagtcc
     2221 acacgcttcg actcctcctc gtgctcgcgg aagttccccg ccgtgtggaa gaacttcttc
     2281 ggcgcgatcg gagcgcgcag ccgcgtttcg gcgagggcga cccgctcgcc gctctcgtcg
     2341 gcgccgccgc gctcgaagta ctcacgcatc gtcggcacgt cgagcacggc gatctcgtcg
     2401 ccgtcgagga acccgacccg gccgtcccct tcgccgaagg tgaccagctt catgccgcga
     2461 ccccgcatgc tctcatcgca gccaacgcgt agacgtgtgc catcttcacc agcccttcga
     2521 tgtcgaggtt ctctccgtag gtcgtgtcga gcaagcccgt cgacgtcccg tagttgacgg
     2581 tcggaacgcc gtagcgcgtg agcgcggacg cgtccgagaa ccaccgcgtc acgtcccgct
     2641 ccggaggcga gccgaacacc tccgtgtgcg acgcatcgat cgcggtgatc agcggatggc
     2701 cctcgtcgat ctcggcgccc ggcgcggtga cgtacacctc gccctcgacc ccgtagtccg
     2761 ggaactgcga ggccaggccg cgcaccatgt cgagcacctg ctggcgcgcc accggcatcg
     2821 gcttcgtcgg cggcacgcga acgtcgagga agagatcggt gcggtgcggt gtccgtgaga
     2881 cgcgccagcc gaagccgccc tgcaccgctc cgacgttgac gatcgccttc gcgccccggt
     2941 gcgcgttctc ggggtcgtcc tcccaggtcg ggatccagtc gagcaccgcg tcgagcacct
     3001 cgcgcatgcg cagaatcgag ttgagctccc gcttaccctc gctgaacgcc gtgtggatga
     3061 agttgccctg cgtcgagata cgcagccaga tcgagccgaa atgcccgagc acgaccttcc
     3121 cctctgtcgg ctcaccgagg atgcacatgt ccgcgagacc gccgtgcgtg acgaggtagc
     3181 gcgaccctgc cgcgtaaccc cggtactggg ccccgatcgc gtcgccctgc tgcgtcttct
     3241 cgatctcgcc gcagacggcc gcgatcatga cgtcgccgcg caggcgaacg ccggcgtcct
     3301 gcaacgcgcg aaccgcctcg acgtagcacg cgagcgcgcc cttcatgttg gagatgccga
     3361 ggccgtagag ccgaccgtcg cgaacgaacg cctcgggctg gaagcccttt acgccagcca
     3421 gccacggctc gttgccggag taggacgtgt ccatgtggcc gttgaacatc aggctcgagc
     3481 cgccgcccgt tccctcccag atgccgatcg cattcgcgcg gccgtcctct acctgctgcc
     3541 actgcacgcg caggcccagc tcgcgatagg tctctgccat cagccgcgcc atggcgagtt
     3601 cgtcgccggt gaagctgggc acgccgatgg cacggctcgc ccagtcgacc aagcgttcgc
     3661 ggtcgatcgt cacggcggcc acgacgccat cggtcagagt cccagccctc cgatcgtcgt
     3721 cccgatcacc tcgaggtagg ccgggaagtc gaagtcgggc ggcgatgcgc cgtccggaaa
     3781 atgaccctcg tagagcggga accacgcgtt cggcgccgac gggttcagga cgaccgtggt
     3841 ggcgcccact gcccggtagg cggcgacacg ttcccacacg tgctcgggcg acccgctgag
     3901 ggtgagggca tcggccatct catcgggcac cagcgcgacc gcagcgtcga cgtcggctgc
     3961 cgcgaccagc gcctcgatgt cgttcatctc cgcctcgaag ccggcgacgg tgacgctcgt
     4021 ctggtacgag acggacgatt tcgtcgcagt ggtgaacatc acgacctgac ctttcgcctg
     4081 ctcgattccc ctcatgtccg gggtcacgac cgaggggaag cccacggcca catcgaaccc
     4141 gtcgagtgaa cgaccggcgc gctccgcgcc gatgcggaga ttcggcatca cgacgtcccg
     4201 caggtattcc accgagtaga agtagccgag gatcccatcc gccagctcac cggcgagctg
     4261 cgccatcttc gggccgatcc cggcgaggta gagagggagc cgcatgtcgc tgggcggcat
     4321 catcagctgg aagcccttga cgcggaagat ctcgccctcg tacgtcgtca actccccgtc
     4381 gacgagccgc cgcatcatcg tcaggtattc gcgcacggcg cgaagcggac gctcgtactc
     4441 gcggccgtgc cagttgtcta cgtacgtttg cgattgcagg cccacgccca gaacgaagcg
     4501 tccactcgag agtcggtgga ggctcgcggc cgtcatcgcc gtcgttaccg gcgagcgcgc
     4561 ccagaggccg acgatgccgg tcgcgatgcc gatacgagtc gagcggcatg cgatggcagc
     4621 ggccggtacg aaggcgtcac cgaaggtgat ctccgagagc cacacgttct tgaagccggc
     4681 ctgctcggcg tggccggcga agtcgagcat gacgtcgact ggcagcgcct gcaggtagac
     4741 gccgagaccg ttgtcgacct gcccggtcat gccgtcatcc cgaccggaag tgcggcagca
     4801 ccttctcgcc gaacaagcgg atctgtccgt gccggtcgcc gccccacagc tcgagcatga
     4861 cgtgggtgca gccggcttgg cggtactcct caatcgcctc gatgcagtcc gccggcgttc
     4921 ctgcgatcgg cttgcaacgg tcgaggatcg agtcggtcac ctccgccttg gccgccagcc
     4981 gtccgccgcg ctccatcgcc tcggccacgg gccggatctc ctccgggtcg atctcggcga
     5041 gctcgagcag cgtgtcggcc gagccgcgaa tgttctgcac cttgttggcg aggtacatgc
     5101 cggcgatctc gcgcgctccg tcgcgcccgg cttcacgatc ctccccgatg gaagccacga
     5161 tcgtgcaccc gatgtcgacc tcgtcgggat cgcgccccga tacggcggcg cccgtctcca
     5221 cgttctcacg cgtgtagcgg acgaaggccg gggtggtgat cgacggcgtg agcagcccgt
     5281 cgcctatctc accgcccagc tgttgcatca ggggcgccgt cccagcgacg tagacgggta
     5341 cctcgccgcg gggcgagtgg gcgtcgtccg acagcgcggg cacgtctgct ccgaagactc
     5401 gcccctcgta ctcgaaccgc tggccggaca ggacgccgcg gacgatctcg accgagtccc
     5461 gcatgtgtgc cagtggcttg gtcaccttgg gcggaccgtc ctgcttcgtg ttgttgagga
     5521 agatcttcga cgttccgaag ccgacgatga atcggtccgg ccccgccgcc tcctgcacga
     5581 cgcgggcgtc catcgccgcc tgcaccggat gccgcgtgta cggcgagatg atcccccacc
     5641 cgatcgtcag gcgctcggtc tcgcgggcca tcagtgccga gacgaccgtc gacgaccggg
     5701 cttccatctg gtgcttccgc caccagggat aggcctccgc gagccagacg gtgtccatcc
     5761 ccgcctcgtc catcacgcgc gccgtctcga gcatctcatc gagcggctcc atcgtcagtt
     5821 gcatgacccc gatccggaag ggagctccca cgcgcatcag cctttcacac gccgggcccg
     5881 cagtccggcc gtcggtgaca tgtcacgggc gcgggtatca ctaacatgtc accggtccga
     5941 cggcaaccga atgccgctca ccgcgcgagc cagccaccgt ccaccgcgag gacgtggccg
     6001 tgcacgtact ccgcttcggg cgagagcagg aaagcgaccg cgcccgcgac gtcctcgggt
     6061 cgcccccagc gcccggcggg gatgtgcgag tggatctcgg cggcgcgctg cggatctgcc
     6121 cgcagcggct cggtcagctc gctctcgacg taaccgggag cgacggcgtt gactcggatt
     6181 ccgagcggtg cccactcgtt ggcgagggcc tttgtcagca cggcaacgcc tcccttgctc
     6241 gccgcgtagg cagcaacgtt cacgcctccc tggaaggaga gcacggaggc catggtgacg
     6301 atggagccgc cggtttgctg agcgacaaaa cgacgggccg ccgcgcgggc cgtgacgaag
     6361 acgctcgtca ggttgaggtc gatcgtcctc cgccacgcgt cgagggggtg gtcgagcgcc
     6421 gggtgacgct cgatcgtgcc ggcattgcac accacggcat cgagccggcc gagcgcgtcc
     6481 gcggcggcgt cgacggcttc ctctgcggcg gaagcgtcgg cgaggtccgc ctgaaggacg
     6541 acggccctac acccgagtgc ctcgatctcg cgtcgcgtct cgatcgcgtc gccgcgctgc
     6601 accagggcaa gatcggcacc cagtgacgcg aggtgaaccg cgacgccctg tccgattcct
     6661 cgaccggcgc ccgtgacgag aacggctcgg ctcgcgagcc agtgcggcgc cgagctcaag
     6721 cggacgtttg ctcggcgacc tgcttctcca gcgcggtcaa cacattcgag acctgctggt
     6781 tcacgatcgg ctggagaacc cgctgcccca tcgacccgac gggcccggcg atcttcacgt
     6841 ccgcctccca gtccatggtc gtcgcgtctc cttcgccgct gagggtgaat tgggtcgtca
     6901 tgttcatcat ggcgccgacg ccggtgccct tggcgttcag tgacgcgtac tcgagcgggc
     6961 gctcttccac cttctcgaag gtgatcgtca tcttcagccc gccaagaccg aggggcacct
     7021 tcactttcgc cgtccagtgc ctgtcgtcga cgatctcgaa gctctcgata ccgggcatca
     7081 ggcccgccat ctgctcgggg tcgttgatca cgctccagac gacctcccgg ggagcctcga
     7141 agtctttcgt tccctcgatc ctcatctgga caccttcgcg ctcgtctccc cttgcagcat
     7201 ctcccacacc cggtggtacg ggttgtggct gtcgaagacg tatacctcgt gaccgccact
     7261 tcgcagcgca tcctgcagtg ccgagcagat gcagtggatg ccgccccctc cgccttctcc
     7321 catccctttg gcgccgagcg gcgtgaacgg ggacggtgac tcgatcgccg acgtcttgag
     7381 cggcggcatg tcgagggcgt ggggaacgtg gtagtcgtag aagttcgggg tgaggaggtt
     7441 tccctcctcg tcgtaggcga acgtctccca gatcgcggcg ccgatggcgt gcgcggtcgc
     7501 ccccatcacc tgtccctcca cgattctggg gttgatccgg gtgccgcagt cgtcgacggc
     7561 cgcgtagtcg acgagctcgt agtagccggt ctccgggtcg acctcgacga cacaggcgtg
     7621 aacctgcgtc gcgtacgtga gcgtgaggtt tccgaacttc ttctccttgt cgggaacctg
     7681 gaacggcggc cggtagacga agcggcggtt catcgtcggc ggatcctcgg ggaagccggc
     7741 gttgttggca ttggggatcg cgcccagggc catgaacggc agtgccgcct cgggattgtc
     7801 cttacgacgc gcaaatccgt ccgccagctc gatgtcgtcg ggaccgtccg ccccgagcac
     7861 catcgccgcg agcagacgca tgtcgcccgc gagctcctcg gcggctcctt tgaccgcgcc
     7921 gaggccggtc acggcgaact ggctcgcgta cgtgcccgag aagccagcat ggctgttcca
     7981 gtagctgtcg tgtccgaccc ggacggtgac ggcatccacg tcgcagccga ggatgtcggc
     8041 gacgacctgg gacgcagtcg tctcgtgccc ctggccctgc ggtgtcgtcc cgagcgtcac
     8101 cacgacctcg ccgaagatgt cgagcttcac ggtggcgacc tcgttgttgc ccgagaactg
     8161 gagctccgga ttgatcagct gggactggcc gaagttgttc gttcccgagt cgagcgtcga
     8221 gccgatcccg atcccgagta gcttgccccg ccgctttgcg tcctcgcgtc gctcgtcgat
     8281 cgtgtcgtac cccaccagct cgagcgcgat atcgaggcag cgggcgtagt cacccgagtc
     8341 gtagacgcac ccgttcggcg tctcgtacgg catctcttcg gcccggacgt agttcttctt
     8401 ccgcatctcg acgggatcga tgccgagctc ggcgccgacg atgtcgacga gccgctcaca
     8461 gaaccagagg tgctgcatcc gcgagtagcc gcgattcgga gacacgggcg ccttgttggt
     8521 catgacctgc gtgaactcga gcttgatgtt gcgccagcgg tacatgcccg gcgtcacctg
     8581 cgaccagatc accccgccga gcggctcgta ccgcaggtac gcgcccgcgt cgtcgagggc
     8641 cttgcagctg aagcccagaa gcgttccgtc ggatttcgcg tacacctcga tgtcctggaa
     8701 ccagcgctcg ttgccgtgcg acatcgacag gtggaactcc gtacgccatt cggtccactg
     8761 aatcggccgg ttcagcttgc gggcgagcag gcagcaggca acgagctgcg ggtgcgacgt
     8821 gatcttgttc ccgaagccgc cgccgatgtc ctgtgtcacg acgcggagct tgtcgatccc
     8881 gacccggagc gcgggcgcca tcatgatcac ggcaaagccg ggaaactgat tgtttgtgta
     8941 cagcgtccac tggccggtgc cgcggttgta ctcgacgagg gccccgtcgg tctcgagcgg
     9001 tgtcgagttg aagcgatcga aatggagctg ctcgatctcg atcacgtggt cggcctcggc
     9061 cttggccgac tccaggtcgc cccactcgta cgttccctcc cacgagaggt tgccgtcggc
     9121 ttcgtcgtgc aacaccggcg cgccgtcatc gagcgcgcgg cgcgcgtcga ccaaggcgtc
     9181 tagcggctcg tactcgacct cgacgagctc ggaggcgtcg cgcgcgagct cgcgggatgc
     9241 agcgacgacg gcgacgatcg gctcgccgac gtagcgagcc ttgcccaccg cgagcgcata
     9301 gtccttgacg ttcgcgccgg gcgggctcgc gatctggaag aacgggtcgg tcaggatcgc
     9361 aacctcgtcg cccgtcagcg tcccgaacac gccctgtagc tccagcgcct tcgagacgtc
     9421 gatcgacacg attcttgcgt gggcataggg cgagcggacg aagtggacga acccggtgtt
     9481 gtggcgcttg atgtcgtcga cgaacacgcc ctgcccctgg acgagtcgcc ggtcctcctt
     9541 ccgtggcacg ttctgaccga cgaatccctt ctcgagcgtc gtctgctgct cgatgagcgt
     9601 cgtcgtcacg agccgccccc cgccttgaca accgcctcga tgatgttcca gtagccggtg
     9661 cagcggcaga ggttgccctg aagcgccttt ctgacctccg cctcgtcagg cgacgggttc
     9721 tcggcgagca gcgcagtcgc gctcatcagc atccccggcg tgcagtagcc gcactggagc
     9781 ccgtggtggt ctgcgaatgc ctgctgcagc ggcgtcagct cgtcgccgtc ggcaagccct
     9841 tcgaccgtcc gcacgctgtg gccctcggcc tgcaccgcga gcaccatgca gctcttcacc
     9901 ggcagcccgt cgaggtgcac ggtgcatgcg ccgcagttcc cggtgtcaca gccgatgtgc
     9961 gtgccggtca ggtcgatgtc gtcgcggatg aagtgcacga gcaacctgcg gctctcgacc
    10021 tcgcgctcgt agaggtcgcc gttgacctcg accgagatgg tccgactgag ggacggtgtc
    10081 tccgccacta ctaccccttc cttctatcgg ctgccgacag gacagcgcgg acggccgaga
    10141 cctcagccag ccgtcgccgg tactcggcgg acgcgtgcac gtccgagggc ggatcgagcg
    10201 ttgcgccaag acccgcggcg gcagcctcca ccgaggccgg cgagagatct cctccggcaa
    10261 gcgcctcctc catcgcagtc gcccgaaccg gtaccgcatc gacgcagccg agcgcgaccc
    10321 gcacggttcc gttcgacact gaagccgcgg cattcgcaac gtacgtgccg tcccgtccga
    10381 gcgacacgcc ttcgaacccg tcgcccgtc
//
LOCUS       DBNGNK010000200         7777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_967269,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000200 DBNGNK010000000
VERSION     DBNGNK010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_967269"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..906
                     /locus_tag="AC150V_05895"
     CDS             292..906
                     /locus_tag="AC150V_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3762490.1"
                     /translation="MSTITRHRIAIADPPALLEIEANAPDVLKRHFALIREGVGRIPG
                     VTPEQAQVGFETAPTLRALGLKPGIFTGVFLAEYHAGKEGEVSSAIKELLATAVSART
                     EADENALCTPYHAGAARLEGAGETDVAIAQDFDARKEELGEETRTVIEFGLKAAYTPA
                     AVTDSDVERVRALGYSDAALVEIVVTALVAYTLAAVNQVFDLSL"
     gene            1125..2171
                     /locus_tag="AC150V_05900"
     CDS             1125..2171
                     /locus_tag="AC150V_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3762491.1"
                     /translation="MTTIDSLIGSEDVRQLLESTEQSGTVRAADIAEFVTLHELAPLE
                     QEALLREFDTRGIEVVDETAAEPVRPLPPPSPVESTTDALQLFLREAGRHQLLTAAEE
                     VELAKRIERGDMTAKQAMIESNLRLVVSIAKNYRNQGLPFLDLIQEGTLGLIRAVEKF
                     DWRRGFKFSTYATWWIRQAVARALADKARTIRMPVHVVERLQKMNRAERTLWTQLGRE
                     PTIEEIADEAAISLQQAVEVRAAARASTSLDAPVGEDDDAVLGDFVSGNDPQPDEVVE
                     LNLRSQALRQALESLPERERSVVFLRYGLGGNEPSTLEEIGRRLGLTRERVRQIELES
                     LRRLAAVREMQSVV"
     gene            complement(2175..3071)
                     /locus_tag="AC150V_05905"
     CDS             complement(2175..3071)
                     /locus_tag="AC150V_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3762492.1"
                     /translation="MEGNAAEPLFDQADAKKGGDEPSEPATQDPLKLYVRQIGDGRLL
                     TPAEERELARRKDLGDEEAKRKLIESNLRLVMSITRNYTKAGVPLLDLIQEGNLGLIR
                     AVEKFDYTLGFKLSTYATWWIRQAISRALAEQGRTIRLPVHVADQVRKVTRARRVLAQ
                     KLSRDPTIDEIAAECDFESQRVQELLELVQDHVSLETPIGEGDSSVADLIEDENADLP
                     EDRTAQNLRSAELEQALAKLKPRQQRVLIERFGLGNANPKTLEEVGKILGITRERVRQ
                     LETRALRELRQVAPGLELYLRS"
     gene            3280..4080
                     /locus_tag="AC150V_05910"
     CDS             3280..4080
                     /locus_tag="AC150V_05910"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR3762493.1"
                     /translation="MCGIAGYSASSTSSIERTLAAQALLAGIAERGEDAVGYAHRSGA
                     SATTVHKQQTGASRMLENVWVAADVGQVLVHVRDFTKGHPSLDVNNHPIRHGAVVGVH
                     NGIIANDEEVFARHGFEREWPGMTVDSEAIFALVEATTSDPLCLEELHGSMAAAWFDE
                     RDAETLHVARGIGRPLWVGEGRSEVFFASTEAALEVVERSLRLQLLKAEVREGSLLRL
                     VGGRIESHASFEPDHSYEDAQQLPAVRAPREGAYCLERMAVLAAWTMA"
     gene            complement(4210..4896)
                     /locus_tag="AC150V_05915"
     CDS             complement(4210..4896)
                     /locus_tag="AC150V_05915"
                     /inference="COORDINATES: protein motif:HMM:NF037417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homing endonuclease associated repeat-containing
                     protein"
                     /protein_id="HIR3762494.1"
                     /translation="MARPRRRHPSLETVDEAALAEFRAGLRRRYTDAEILEELRASAE
                     RLEKSPTMREFAADGEARMHPQTVIEHFGTWNAAKREAGLTPRRFISREELLRQLREL
                     GDDLGRTPTARDLDENRGRVASKSLIWHTFGSLTNALREAGFDVPLAEERLERAVADG
                     AVLARRLGRLPRMADWREARTKDTEMLSEWQVYRMVDVSPGAWSAFQFLVRERLREEG
                     VGVASDGSLA"
     gene            4979..6352
                     /locus_tag="AC150V_05920"
     CDS             4979..6352
                     /locus_tag="AC150V_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HIR3762495.1"
                     /translation="MRTRLAIEAAAAQAVGKLSRAARRGGGTTLPGKLLWKVDPGAVD
                     ELASRLPAGVALVSATNGKTTTAAMAAEILGRRRELAWNHSGANLASGVASTLLASRG
                     AELGLLEVDEFALPEVMRRIRPRTVALGNLFRDQLDRYGELEHVAERWRSAVAALAPD
                     AELIVNADDPLVAALADDRQGVTRFGVDDRRRARPSLQHAADSKYCVRCGTPYRYEAA
                     YVGHLGEYVCDRCGQRRPPLDLAARQVEPRGLEGTSFELETPSGAISVNLRLPGLYNV
                     YNALSAAALALAVGASLDEIQSGLDAFSAAFGRFERIDAGDRSILMLLIKNPAGANEA
                     VRTLEEGGIPPVLVLALNDDIADGRDVSWIWDVDFEPLLHGTRRVVASGERAEELALR
                     FVYGGVERERVEVVPQLEAALDRGLELVGPGGELAVLPTYTAMLHLRSIAVDRGLVRP
                     YWEKTPA"
     gene            6349..7101
                     /locus_tag="AC150V_05925"
     CDS             6349..7101
                     /locus_tag="AC150V_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR3762496.1"
                     /translation="MTIRVGHLYPDYLNIYADRGNIAVLRRRAGLRAHVLEVAAVGLG
                     EPLVAGAYDLLYIGGGQDREQAVIAPDLAEKGAAIRAAVEDGCALLAVCGGYQLLGRG
                     YRGRDGSFMPGVALFPHETVAGEKRMIGDVLLACELVPGEPRTLAGFENHAGRTRLDP
                     GAEPLGRVISGFGNDGESGYEGARVGRAIGTYLHGPLLPRNPWLADWLLAEALAHAGG
                     GEREMLEPLDDELELAAHRVAERRARERGGKY"
     gene            complement(7106..>7777)
                     /locus_tag="AC150V_05930"
     CDS             complement(7106..>7777)
                     /locus_tag="AC150V_05930"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3762497.1"
                     /translation="GACDQFAELVPRAHDHGSWVHVDGAFGLWAAASPAHAHLTAGHE
                     AADSWATDAHKWLNVPYDSGLVYVSQPDAHFAATRFQDAPYLESVQDGERDQAEWTVE
                     SSRRARGFAVWAALQSLGRRGVAELVNRCCAHARRLGSLLSAADGLEILNDIVLNQVL
                     VRVGDSDEATRAALRRVQADGTFWCGGTIWQGRAAIRISVSGWQTSELDIERSAEALV
                     RAARR"
BASE COUNT         1241 a         2655 c         2641 g         1240 t
ORIGIN      
        1 tcgaggtgct gctcggctac gtccgccgcc acgactacac ggtgttcgtc gtgttccgcc
       61 tcgtcgcagc ggcgatcgtc gtcctgctca tcgtgaccgg ggtgcgcgcg gcgacgttct
      121 agcgccctag gacagggcag agaggccccg tacgcgcgtc gcacggcctc cggtgtcccg
      181 gacacgcgcg agatcgtgtc gaaccaccga ccccgagcgg tgttgtccgc cgtcacttca
      241 ttctgtaaag tcgcttcaca tagcaaagca ttggaccgga gaaagggaga catgtcgact
      301 atcacacggc accgcatcgc aattgccgac ccgcccgccc tgctcgagat cgaggcgaac
      361 gcccctgacg tcctgaagcg gcacttcgcg ctcatccgcg agggagtggg tcgcattccg
      421 ggcgtcaccc ccgagcaggc ccaagtcggc ttcgagacgg cgccgacgct gcgcgccctc
      481 gggctcaagc ctgggatctt caccggtgtg ttcctcgccg agtaccacgc gggcaaggag
      541 ggcgaggtct cgtctgcgat caaggaactg ctggcgacgg cagtgtccgc gcgcaccgag
      601 gccgacgaga acgcgctgtg caccccatat cacgcaggcg cagcacgcct ggaaggcgcc
      661 ggcgagaccg acgttgcgat cgcgcaggac ttcgatgcgc gaaaagagga gctcggagag
      721 gagacccgca cggtgatcga gttcgggctc aaggctgcct acacgcccgc cgccgtaacg
      781 gactccgacg tcgagcgggt gcgggctctt ggctacagcg atgcagcgct cgtcgagatc
      841 gtggtcacgg cgctcgtcgc ctacacgctc gccgcggtca accaggtctt cgacctgagc
      901 ctctagacgc caccgcaacg cttccggcgg tcggcggtgg cccgcacact gggctcgtcg
      961 cgaacacggg gcggaggagg gcgagatccc agcagatatg gggccccttt cttctacttg
     1021 acataacacg cgttatcgtg cgcgaccgag aacacgaggg tcgcggactg gacacggcgg
     1081 cgccgacgcg agaaagtctc acttgcaacg caaccctctt ccacatgacg accatcgaca
     1141 gcctgatcgg ctccgaagac gtgcggcagc tgctggagtc gacggaacaa tcgggaaccg
     1201 ttcgtgccgc cgacatcgcg gaattcgtca cgctgcacga gctggctcca ctcgagcaag
     1261 aagcgctgct gcgcgagttc gacacgcgcg ggatcgaggt agtcgacgaa actgccgccg
     1321 agccggttcg tccgcttccc ccacccagcc cggtcgagag cacgacggac gcgctgcagc
     1381 tgttcctgcg cgaggcaggg cgtcatcagc tcctgaccgc ggccgaggag gtggagctcg
     1441 cgaaacggat cgaacggggt gacatgacag cgaagcaggc aatgatcgag tcgaacctgc
     1501 gcctggtcgt ctcgatcgcg aagaactacc ggaaccaggg tctgcccttc ctcgacctca
     1561 tccaggaggg cactctcggc ctgatccgcg cagtcgagaa gttcgactgg cggcgcggct
     1621 tcaagttctc gacctacgcg acatggtgga tccgtcaggc agtcgcccgc gccctcgccg
     1681 acaaggcgcg aaccatccgc atgccggtac acgtcgtcga gcggctgcag aagatgaacc
     1741 gggcggagcg gacgctatgg acgcagctcg gccgcgagcc caccatcgag gagatcgccg
     1801 acgaggccgc gatctcgctc cagcaagcag tcgaggttcg ggccgcggcg cgcgcctcga
     1861 ccagcctcga cgcgccggtc ggcgaggacg atgacgcggt cctgggcgac ttcgtgtcgg
     1921 gcaacgaccc gcagcccgac gaggtggtcg agctcaacct tcgcagccag gcgctccgtc
     1981 aggcactcga gtcgctcccc gagcgtgagc ggagcgtggt gttcctccgc tacgggctcg
     2041 gcggcaacga gccctcgacc ctcgaggaga tcgggcgtcg cctggggctc acccgggagc
     2101 gcgtccgcca gatcgagctc gagtcgctac ggcggctcgc ggccgttcgc gagatgcagt
     2161 cggtggtcta gccgctacga gcggaggtag agctcgaggc ccggcgcgac ctgtcgcagc
     2221 tcgcgcaggg cgcgcgtttc gagctgccgg acgcgctcgc gggtgatgcc gaggatcttg
     2281 ccgacctctt ccagggtctt cgggtttgca ttcccgagtc cgaagcgctc gatcaacacc
     2341 cgctgctggc gaggcttgag ctttgcgagc gcctgctcga gctcggccga gcgaaggttc
     2401 tgggcggttc gatcctcggg caggtctgcg ttctcgtcct ctatcagatc agcaaccgag
     2461 ctgtcgccct cgccgatcgg ggtctcgaga ctcacgtgat cctgaacgag ctcgaggagc
     2521 tcttgcacgc gctgggactc gaaatcgcac tccgcggcga tctcgtcgat cgtcgggtcg
     2581 cggctcagct tctgggcgag gacacgcctc gccctcgtca ccttgcgaac ttggtcggcg
     2641 acgtgtacag gaagacggat cgtccttcct tgctcggcga gcgcccgtga gatcgcctgc
     2701 cggatccacc acgttgcgta ggtcgagagc ttgaagccga gggtgtagtc gaacttctcg
     2761 acggcacgga tcaagccgag gttgccttcc tggatgaggt cgagaagcgg gacgccggcc
     2821 ttcgtgtagt tgcgcgtgat cgacatcacg agccggaggt tcgactcgat cagtttccgc
     2881 ttggcttcct cgtcgcccag gtccttccgg cgggcgagct cccgctcttc cgccggggtc
     2941 agcaggcgac cgtcgccgat ctggcggacg tagagcttga gcggatcctg ggtggcgggc
     3001 tcgcttggct cgtcgccgcc tttcttggcg tcggcctggt cgaacagggg ctcggcggcg
     3061 ttgccctcca cgggggctgt atcggcgccg atgggccctc gcttgaggga tggacgaaaa
     3121 ccgcgcgctt ccgcctaccc gcacgggcgt tcagccagaa caggcgcgtg ccgatgcaag
     3181 cggtaaacaa actgtgaact cccacgtacc gggtgtaact tcgacgccag cgtcgtcgtc
     3241 tgagttgatg tccgagatct gtggtgggat ctgcggccta tgtgtggaat agccggatac
     3301 agcgcgagca gcacgtcatc gatcgagcgc acgctcgcag cgcaggcgct gctcgccggg
     3361 attgccgaac gcggcgagga cgccgtcggc tacgcccatc ggagcggcgc ttcggcgacc
     3421 accgtccaca agcagcagac cggcgccagc cgcatgctcg agaacgtctg ggttgccgcc
     3481 gacgtcggcc aagttctcgt tcacgtccgt gacttcacga agggccaccc gtcgctcgac
     3541 gtcaacaacc acccgatccg ccacggcgcc gtggtcggcg tacacaacgg gatcatcgcc
     3601 aacgacgaag aggtcttcgc tcgtcacggc ttcgagcgtg aatggccggg catgacggtc
     3661 gactccgagg cgatcttcgc cctcgtcgag gcgaccacga gcgacccgct ctgcctcgag
     3721 gaactgcacg gctcgatggc cgcagcatgg tttgacgagc gcgacgccga gacgcttcac
     3781 gtcgcccgcg gcatcggacg cccgctgtgg gtcggcgaag ggcggagcga ggtgttcttc
     3841 gcctcgaccg aggcggctct cgaggttgtc gagcgttcgc ttcgcctcca gctgctgaag
     3901 gccgaggtgc gcgaagggag cctgctgcgg ctcgtcggcg gacgcatcga gtcgcacgcc
     3961 agcttcgagc ccgaccacag ctacgaggac gcgcaacagc tgcccgccgt gcgcgcgccc
     4021 cgcgaaggcg cctactgcct ggagcggatg gccgtgctcg ccgcctggac gatggcctag
     4081 cgcggctcag acccgcgttc acgacaccca acggctcgag cacgctcgcc gcaggacgac
     4141 gcgactctgc ggcacctgac ccgtcccccg cggcgtcgta cgcctgggct cagctcgagc
     4201 tgagggcggt caagcgagcg atccgtccga ggcgacgccg acgccttcct cacgcaaccg
     4261 ttctcgtacg aggaactgga aggccgacca cgctccgggg ctgacgtcga ccatccgata
     4321 gacctgccat tcggacagca tctccgtgtc cttcgtgcgc gcctcgcgcc agtcggccat
     4381 cctcggcagt cgcccgagcc ggcgggccag aacggctccg tcggcgaccg cccgttcgag
     4441 tcgctcctcg gccagtggga cgtcgaagcc cgcctcgcgc aaagcgttcg tcagagaccc
     4501 gaacgtgtgc cagatgagag acttcgaggc tactcggccc cggttctcgt cgaggtctcg
     4561 cgcggtcggc gtccgaccga ggtcgtcacc gagctcacgg agctgccgga gaagctcctc
     4621 gcggctgatg aagcgtcggg gcgtcaaccc tgcctcgcgc tttgccgcat tccacgtgcc
     4681 gaagtgctcg ataacggtct ggggatgcat ccgcgcctcg ccgtccgctg cgaattccct
     4741 catcgtcggc gacttttcga gccgctcggc gctcgcgcgg agctcctcga gaatctccgc
     4801 atccgtatag cgccggcgca gacccgcgcg gaactcggcc agcgctgcct catcgaccgt
     4861 ttcgagtgac ggatgccgcc gtcgcggacg ggccatgcaa agactgtaga gcacactggg
     4921 aatttcgcaa gtccgcccga acgcggtcct gccgtttccg gcgggtacgc tcgacgccgt
     4981 gcggacgcga ctggcgatcg aagcggcggc tgcccaggct gtgggcaagc tctcccgcgc
     5041 cgcgagacgc ggcggtggca cgacattgcc cggaaagctg ctctggaaag tcgatccagg
     5101 cgccgtcgac gagttggcct cccgcctgcc ggccggagtc gcgctcgtct ctgccaccaa
     5161 cggtaagacg acgacggcgg cgatggcagc ggagatactc gggcggcgcc gcgagctcgc
     5221 ctggaatcac tcgggcgcca atctcgcgtc cggagttgcg tcgaccctgc tcgcgtctcg
     5281 cggagccgag ctcgggctgc tcgaggtcga cgagtttgcg ctgccggagg tgatgcgacg
     5341 gatccggccg cgaaccgtcg cactcggaaa cctgttccgc gaccagctcg accgctatgg
     5401 agagctggag cacgtggcgg agcgttggcg ctcggcggtc gccgcactcg ccccggacgc
     5461 tgagctgatc gtcaacgcgg acgatccgct cgtcgcggcc ctcgccgacg atcggcaagg
     5521 cgtcacgcgt ttcggagtcg acgatcgacg tcgggcacgg ccgtctctcc agcacgccgc
     5581 cgactcgaag tactgcgtcc gctgcggtac cccgtatcgg tacgaagccg cctacgtcgg
     5641 ccatctgggc gaatacgtgt gcgaccgctg cgggcaacgc cggccaccgc tcgacctggc
     5701 tgcgcggcag gtggagccgc gagggctgga aggaacatcg ttcgagctcg agacgccgtc
     5761 cggggccatc tctgtgaact tgcggctacc gggcctctac aacgtctaca acgcactttc
     5821 ggcagccgcg ttggctctcg ccgtgggggc ctcgctcgac gagatccagt cagggctcga
     5881 cgccttcagt gcggcgttcg gccggttcga gcgcatcgac gccggagacc gcagcattct
     5941 catgctgctg atcaagaacc ctgccggcgc caacgaggcg gtgcggacgc tggaggaagg
     6001 gggcattcct ccggtgctcg tgctggctct caacgacgac atcgcagacg gccgcgacgt
     6061 ctcgtggatc tgggatgtcg acttcgagcc cctcttgcac gggacacgca gggtcgtggc
     6121 gagcggagag cgtgcagagg agctcgccct gcgcttcgtc tacgggggcg tggagcggga
     6181 gcgggtcgaa gtcgttccgc aactcgaggc ggcgctcgat cgggggctcg agctggtcgg
     6241 gcccggtggt gagctcgccg tcctgccaac gtataccgcc atgctccacc tgcgctcgat
     6301 cgcggtggac cgaggtctcg tgcgtccgta ctgggagaaa actccggcat gacgatccgc
     6361 gtcggacacc tctacccgga ctacctcaac atctatgcgg atcgtggcaa catcgcggtg
     6421 ctccggcgac gcgccggcct gcgagcgcac gtactcgagg tggccgccgt cgggctcggc
     6481 gagccactcg tcgccggggc ctacgacctc ctctacatag gcggagggca ggatcgggag
     6541 caggcggtca tcgcccccga tctcgccgag aagggcgcag cgatcagggc ggccgtcgaa
     6601 gacggctgtg cgctgctcgc cgtgtgcggc ggctaccagc tgctcggccg cggctaccgg
     6661 ggacgtgacg gctcgttcat gcccggcgtc gccctgttcc cgcacgagac cgtggccggc
     6721 gagaagcgga tgatcggcga cgtcctgctc gcgtgcgagc tcgtccccgg cgagccccgt
     6781 acgctggccg ggttcgagaa tcacgcgggt cggacgcgcc tcgaccccgg cgcggagccg
     6841 ctcggacggg tgatctcagg cttcggcaac gacggcgagt ccggctacga gggcgcgcgg
     6901 gttggccggg cgatcggcac ctacctccac ggcccgctcc tgccccggaa cccgtggctc
     6961 gccgactggc tcctagcgga ggcccttgct catgccggag gaggtgagcg ggagatgctc
     7021 gagccgctcg acgacgagct cgagctggcg gcgcaccgcg tggcggaacg tcgagcgcgg
     7081 gagcggggcg gcaagtatta gcgcgtcaac ggcgtgccgc gcgaacgagc gcctcggcgg
     7141 agcgctcgat atccagttcg ctcgtctgcc agcctgacac ggagatccga atcgcggccc
     7201 gaccctgcca gatcgtgccg ccgcaccaga acgtgccgtc cgcttgcacg cggcgaagcg
     7261 cagctctcgt cgcctcgtcg ctgtccccca ctctgaccag cacctggttg agcacgatgt
     7321 cgttgaggat ttcgagcccg tcggcagcgg agaggagcga acctaggcgt cgcgcgtgcg
     7381 cacagcagcg attcacgagt tccgccacgc ctcgcctccc gagcgactgc agtgccgccc
     7441 acacggcgaa gccgcgtgcg cgccgggacg actcgaccgt ccactcggcc tgatcacgct
     7501 cgccgtcttg cactgattcg aggtacggcg cgtcctgaaa gcgtgtggcg gcgaagtgcg
     7561 cgtcgggctg tgacacgtac acgaggccgc tgtcatacgg gacgttcagc cacttgtgtg
     7621 cgtccgtcgc ccacgagtcg gcggcttcgt gacccgccgt gagatgcgca tgggcagggc
     7681 tggctgccgc ccacaggccg aaggcgccgt cgacatgcac ccacgaaccg tggtcgtgcg
     7741 cccgtggaac gagctccgcg aactgatcgc acgctcc
//
LOCUS       DBNGNK010000201         2597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_466677,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000201 DBNGNK010000000
VERSION     DBNGNK010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_466677"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="AC150V_05935"
     CDS             complement(<1..718)
                     /locus_tag="AC150V_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3762498.1"
                     /translation="MRRYAIWGTATSSTHEAWVRAVAKEFEKDGLVLVDDIAEADFVL
                     NMFDAESPKAFRRASRGTYSAAFYELEDLPEDVLKTSYPMLVRTLANVVVLHVPGKGV
                     WFTTMERGTYEISDDPAEVFERLAPLAKSKLVIDNEFNSDLEPELWDGDEVTVDISAA
                     GRRMGDLDLLPAPFPVEEYLNERDLRHVMRLYQVGGLSYGNLSARKDDTRFWMSASGV
                     DKTKLEVAGRDILLVTGYDEE"
     gene            complement(803..1201)
                     /locus_tag="AC150V_05940"
     CDS             complement(803..1201)
                     /locus_tag="AC150V_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3762499.1"
                     /translation="MHYRWLVAAAVLVALALAGPAQAAAPKVSVKLNEFKVIPKPKSV
                     KGGKVTFTAKNTGSIVHELVVLRTKAAPSKLKTSGAKAVEKGRVGKVQNIKPGKTKKL
                     TLKLKKGKYVLLCNVPGHYQAGQRIGFVVK"
     gene            1422..>2597
                     /locus_tag="AC150V_05945"
     CDS             1422..>2597
                     /locus_tag="AC150V_05945"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762500.1"
                     /translation="MSTGSVGPAGERTRPDSRSVAGPTESEHAEEQRRKATRVLVMAT
                     FGFTVFFAVWVMFAIVGIPLRDELELSSGRFAVLVAIPILTGSVLRVPIGIVTDHVGG
                     RRVFTGLLVATAIPCFFVSRADGYYELLILAFCVGLAGTSFAAGIAWVSVWYPPERQG
                     FSLGMFGAGNIGASITKLLAPTLVTLIAVGGVAGGLIPGGWRFVPFLYSFLLLATAAA
                     TWLLAPGPDRTPARDRTLREVSAPLRHLRVWRFGLYYVIVFGAYVALALWLPKYYVDV
                     YDINLRLAGVLTAFFIFPASLMRPVGGWMSDRIGARPVTYTAFILMCFALVWLSLGVG
                     IVLFTVLVVTVGVMMGIGKASVYKYIADYFPRDVGAVGGLVGAIGGLGGFVLPLLFAW
                     "
BASE COUNT          358 a          894 c          843 g          502 t
ORIGIN      
        1 tctcctcgtc gtagccggtg acgaggagaa tgtcgcgtcc ggcgacctcg agcttggtct
       61 tgtcgacacc gctcgcgctc atccaaaacc gcgtgtcgtc ctttcgcgcg gagagattcc
      121 cgtaggagag gccgccgacc tggtacaggc gcatcacgtg gcggagatcc cgctcgttca
      181 gatactcctc gaccgggaag ggcgcaggca gcaggtcgag atcgcccatc cgcctccccg
      241 ccgcgctgat gtcgaccgtg acctcgtcgc cgtcccagag ctccggctcg aggtccgagt
      301 tgaactcgtt gtcgatcacg agcttcgact tcgccagagg cgccaggcgc tcgaacacct
      361 cggcggggtc gtccgagatc tcgtacgtgc cgcgctccat cgtcgtgaac caaacgcctt
      421 tgccgggcac atggaggacg acgacgttcg cgagcgtgcg gacgagcatc gggtagctcg
      481 tcttcaacac gtcctcgggg aggtcttcga gctcgtagaa ggccgccgag tacgtccccc
      541 gcgacgcccg ccggaacgct ttcgggctct cggcgtcgaa catgttgagc acgaagtccg
      601 cctccgcgat gtcgtcgacg aggacgagcc cgtccttctc gaactccttg gctaccgccc
      661 gcacccacgc ctcgtgcgtg gacgacgtgg cggtgcccca gatcgcgtac ctgcgcacgg
      721 cgaaacctta gcgccgacgg cggcggcccg ggctgcgggc cgcccgccgc tcggctcctc
      781 actcgaaggt gaggccgatg cgctacttga cgacgaagcc gatccgctga ccggcctggt
      841 agtgacccgg gacgttgcag aggaggacgt acttgccctt cttcagcttg agcgtgagct
      901 tcttcgtctt gccgggcttg atgttctgaa ccttcccgac gcggcccttc tcgaccgcct
      961 tggccccgct cgtcttcagc ttgctcggcg cggccttcgt cctgagcacg acgagctcgt
     1021 ggacgatgct cccggtgttc ttcgccgtga acgtgacctt gccgcccttc accgacttcg
     1081 gcttcgggat caccttgaac tcgttgagct tgacactcac cttcggcgcg gccgcctggg
     1141 ccgggccggc cagggcgagg gcgacgagaa cggccgcagc gaccaaccag cggtagtgca
     1201 ttcgacagga cccccttgct cgtgactcca ctccggacat tggagcagga ccgcttatcc
     1261 cgcagtcgca gtaatccgta gttctccgca tggcaagcgg tgaagccgac gatgcaccgg
     1321 catgcgggct cggccagcgt tgcgccgcgt gagagccgat gggcagcgac tgatcacttt
     1381 taaccagccc tcgcgggcgg cggctcgcga gaattcggag catgagcacc ggtagcgttg
     1441 gcccggcggg cgagcgcact cggcctgatt cgcgatccgt cgcgggccca accgagagcg
     1501 agcacgcgga ggagcagagg cgcaaggcaa cccgtgtgct cgtgatggcg accttcggct
     1561 tcacggtgtt cttcgccgtg tgggtgatgt tcgcaatcgt cggaatcccg ctccgagacg
     1621 agctcgagct gagctcggga cggttcgccg tgctcgtcgc cattccgatc cttaccggct
     1681 cggtgctacg agtgccgatc ggcatcgtca ccgaccacgt cggcgggcgt cgggtcttca
     1741 ccggactgct cgtggcgaca gcgatcccct gcttcttcgt cagccgtgcc gacgggtact
     1801 acgagctgct gatcctcgcg ttctgcgtcg ggctcgcggg aacctctttc gcagccggga
     1861 tcgcctgggt ctccgtctgg tacccgcctg agcgccaggg cttctcgctg ggcatgttcg
     1921 gcgccgggaa catcggggcg tcgatcacga agctgctcgc ccccaccctg gtgacgctga
     1981 tcgcagtcgg cggcgtcgcc ggcggtctga ttccgggcgg ctggaggttc gtcccgttcc
     2041 tctactcgtt cctgctgctc gcaacggcgg ccgcaacgtg gctgctcgcg cccggacccg
     2101 accgtacgcc ggcgcgcgac cgcacgctcc gggaggtctc cgcacccctt cgtcacctgc
     2161 gcgtctggcg cttcggcctg tactacgtga tcgtcttcgg cgcgtacgtc gcgctggcgc
     2221 tctggctgcc caagtactac gtcgacgtct acgacatcaa ccttcgcctg gcgggggttc
     2281 tcaccgcctt cttcatcttc ccggccagcc tgatgcggcc ggtcggcggc tggatgtccg
     2341 atcggatcgg ggcgcggccg gtcacgtaca cggccttcat tctcatgtgc ttcgcgctcg
     2401 tctggctctc tctcggcgtc gggatagtcc tcttcacggt gctggtcgtg accgtcggcg
     2461 tgatgatggg gatcggcaag gcgtccgtct acaagtacat cgccgactac ttcccgcgcg
     2521 acgtgggagc ggtcggtggg ctcgtcggcg cgatcggcgg gctcggcggc ttcgtgctgc
     2581 cgctgctctt cgcatgg
//
LOCUS       DBNGNK010000202         3547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_680250,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000202 DBNGNK010000000
VERSION     DBNGNK010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_680250"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="xseA"
                     /locus_tag="AC150V_05950"
     CDS             <1..850
                     /gene="xseA"
                     /locus_tag="AC150V_05950"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR3762501.1"
                     /translation="FRATAIERFGLGAHLAAIERLRRALAAEGLFAESRKRPLPRFPK
                     AVGVLTGSDAAARGDIVTAIHTRFPPARVVVAETRVQGRGAADSIVSALRALSAHEHV
                     EVVVLTRGGGSFEDLLPFSAEPVVRAVAACPVPVVSAVGHEQDTPLCDLAADVRASTP
                     TAAARLVVPDLDELRTDLDRSRAAVGRSVGHLLQRRSDVLSQLRGRLHAAPSLLLERR
                     RSALELAAARLRALSPEATLGRGYAIVRADGAVLRDSAEARTGREIDVQLATGGLLAR
                     VEETRP"
     gene            847..1053
                     /gene="xseB"
                     /locus_tag="AC150V_05955"
     CDS             847..1053
                     /gene="xseB"
                     /locus_tag="AC150V_05955"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005979588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR3762502.1"
                     /translation="MSDEPTFEQAQRELERIVSRLERGDVAVDEAIALWRRGEELYRL
                     CVAKLDTAEGQVANLQADDADKSP"
     gene            1073..1489
                     /locus_tag="AC150V_05960"
     CDS             1073..1489
                     /locus_tag="AC150V_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3762503.1"
                     /translation="MPEPSHDLLRNVPMFSGLDDGQLDALGAEFREREFKAGDRILLE
                     GDEGLTFFVIESGDASVTVRGEEVARLAGGGHFGEIALVDRGPRAATVTATTDVHAHM
                     LPIWNFRPLVESNPDLAWKLLESLAALLREIEDRNL"
     gene            1537..2100
                     /locus_tag="AC150V_05965"
     CDS             1537..2100
                     /locus_tag="AC150V_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3762504.1"
                     /translation="MVESDPALVARCLEGDEDAWDALVDRYARYVHAITVQAFRLAPE
                     DAEDVFQEVFLRAFEHLDSLRRGDALRPWIGQLTRRCCLDRVRSGSREQARGTVDELE
                     DLDDVTARIDEALTLRVELEHLSPDCREVLDRFFGRDESYRTIGDVLELPPGTIASRI
                     SRCLGKLRERLEGRKPSVSTSSIRVTR"
     gene            2097..2348
                     /locus_tag="AC150V_05970"
     CDS             2097..2348
                     /locus_tag="AC150V_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762505.1"
                     /translation="MTGHDEDRIAELLAALPPAPQSWVRAAKELPRARAELDGLVARA
                     EADAEYRAAVLADLERALSASGVEPTRAAVEALRRRLRE"
     gene            2345..2941
                     /locus_tag="AC150V_05975"
     CDS             2345..2941
                     /locus_tag="AC150V_05975"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR3762506.1"
                     /translation="MTGPAAESAFSLQTLETFLDRLGSREPVPASGSAAALVGAMAAG
                     LVEKVARISEEWEDSGGAAAQAMALRRRLLELAPADADAYRGALAAMRLPEDVPKEER
                     DIALGRSLERAADVPLAIADAAADTVELAAEAAEHCRSSVRSDAAAAADLAAGAARAA
                     ARLVDVNLATTPGDPRTTLAEEIASRAAAARERIGTEG"
     gene            2938..>3547
                     /locus_tag="AC150V_05980"
     CDS             2938..>3547
                     /locus_tag="AC150V_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3762507.1"
                     /translation="MSERALPAWSLADEALGDIAVRAGWSERPTREWALGGARGAGVS
                     VCILDSGIDATHPRVGGVESAKAIVVEAGEPLVVDDDEGDVCGHGTACAGIVRHVAPG
                     CEIHSVRILGTKATGSGDSLLAGLRHAVEEGFQVVNLSLSTTKKQLAQMLHELVDAAY
                     FRRTVLVASAHNMPVESYPWRFSSLISVGSHEGGDPLTWYANP"
BASE COUNT          517 a         1236 c         1281 g          513 t
ORIGIN      
        1 cttccgggca accgcaatcg agcggttcgg gctgggagcg caccttgcgg cgatcgagcg
       61 gctgaggcgg gcactcgcag cagaagggct cttcgcagag tcgcgcaaac gcccgctgcc
      121 gcgcttcccg aaggcggtcg gagtgctcac ggggagcgat gccgccgcgc gcggcgacat
      181 cgtcaccgcc attcacactc gcttcccgcc cgcgcgggtc gtggtggcag agacacgtgt
      241 ccagggacgg ggagcggccg attcgatcgt ctccgccctc cgcgctctct ccgcgcatga
      301 acacgtggag gtcgtcgtgc tcaccagagg gggcggctcc ttcgaagacc ttctcccctt
      361 ctcggccgag ccggtcgttc gggcggtcgc cgcctgtccg gtccccgtcg tgtcggcggt
      421 aggtcacgag caggacacac ccctctgcga tctggccgcc gacgtacgtg cctcgacgcc
      481 gacggcggcg gcgcgcctcg tcgtcccgga tctcgacgag ctgcgcaccg acctcgaccg
      541 ctcgcgggcg gcggtcggtc ggagcgtcgg tcatctgctg cagcgacgca gcgatgtcct
      601 ctcgcagctg cgcgggcggc tccacgcggc tccgtcgctc ttgctcgagc gccgccgaag
      661 cgctctcgag cttgcagctg cacggctacg ggcgctctcg cccgaggcga cgctcgggcg
      721 tggctatgcg atcgtgcgcg ccgacggagc ggtgctacgc gactcggccg aagcccgtac
      781 aggccgggag atcgacgtgc agctcgcgac cggagggcta ctggcccgcg tcgaggaaac
      841 tcggccgtga gcgacgagcc cactttcgag caggcgcagc gggagctcga gcggatcgtc
      901 tcgcgcctcg agcgcggcga cgtcgcggtc gacgaggcga tcgcgttgtg gcgccgcggc
      961 gaagagctct atcgcctctg cgtggcgaag ctcgacaccg ccgaaggaca ggtggccaat
     1021 ctgcaggcgg acgacgcaga caagagcccc tgaccctgca agaatgccgc cgatgccgga
     1081 gccgtcgcac gacctcctcc gcaacgtgcc gatgttctcg gggctcgacg acggccaact
     1141 ggacgcgctc ggcgccgagt tccgggagcg cgagttcaag gcgggtgacc ggatcctgct
     1201 cgaaggtgac gagggcctga ccttcttcgt gatcgagtct ggcgacgcct cggtcacggt
     1261 gcgcggcgag gaagtggcga gactggccgg cggcggccac ttcggcgaga tcgcgctggt
     1321 cgaccgcggt ccgcgcgcgg cgaccgtgac cgcgacgacc gacgtccacg ctcatatgct
     1381 cccgatctgg aacttccggc cgctcgtgga gtcgaatccg gacctcgcct ggaagctgct
     1441 cgagtcgctt gccgcgctcc tgcgcgagat cgaagaccgg aacctctagt aacgggccgg
     1501 gtagacctca cgaccggcct aggcttgact tagcgcatgg tcgagtccga tcccgccctc
     1561 gtggcacgct gcctggaggg ggacgaggac gcctgggacg cgctcgtcga ccgctatgcg
     1621 cggtacgtcc acgcgattac cgtccaggcg tttcgactcg cgccggagga tgccgaggac
     1681 gtctttcagg aggtattcct gcgcgctttc gagcatctcg actcgctgcg caggggcgac
     1741 gcgctccggc cgtggatcgg gcagctcacc cgccgttgct gcctcgaccg ggttcgctcg
     1801 ggaagccgtg agcaggcgcg cgggacggtg gacgagctcg aggacctgga cgacgtgacc
     1861 gctcgcatcg acgaggcact taccttgcgc gtcgagctcg agcacctgtc gccggactgc
     1921 cgcgaggtgc tcgaccgctt ctttggccgt gacgagagct accggacgat cggcgacgtc
     1981 ctggagctgc cgccgggaac gatcgcaagc cgcatttccc ggtgcctcgg gaagctccgc
     2041 gagcgcctcg agggaagaaa gccgtccgtt tcgacgtcaa gtatccgggt gactcgatga
     2101 cgggacacga cgaggaccgc attgccgagc tgctggccgc cctgccaccc gccccgcaga
     2161 gttgggttcg cgcggcgaag gagcttccac gggcgcgggc cgagctcgac gggctcgtgg
     2221 ctcgggcaga ggcagacgcg gaataccgcg cggcggtact cgccgatctc gagcgcgcgc
     2281 tgtccgcgtc gggcgtcgag ccgacgcgcg ccgccgtcga agcactccgc cgccggctgc
     2341 gcgagtgacg ggcccggcgg ccgaatcggc gttctctctg cagacgctgg agaccttcct
     2401 cgaccggctc ggttcccgcg agccggttcc ggcgagcggg tcggctgcag cgctcgttgg
     2461 cgcgatggcg gccggcctcg tcgagaaggt cgcgcgcatc agcgaggagt gggaggacag
     2521 cggaggcgcg gcagcccagg ccatggcgct tcggcggcgg ctactggagc tcgcgcccgc
     2581 cgacgcggac gcgtaccggg gcgcgctcgc ggccatgcgc ctccccgagg atgtgccgaa
     2641 ggaggagcgc gacattgcgc tcggacggtc tctcgaacgt gccgcggacg tgccactcgc
     2701 gatcgccgac gccgcagccg acaccgtaga gctcgccgcg gaagcggcgg agcactgccg
     2761 atcctccgtg cgctccgacg cggcggcagc ggccgacctc gccgccgggg cggcgcgggc
     2821 ggccgccagg ctggtcgacg tgaatctcgc gacaacgccg ggcgatccgc ggacgacgct
     2881 cgccgaggag atcgcctcgc gtgccgccgc cgcccgggaa cggatcggaa cggaggggtg
     2941 agcgagcggg ccctccccgc ctggagcctg gccgacgagg cgctgggcga catcgcagtg
     3001 cgggccggct ggtcagaacg acccacccgt gagtgggcgc tcggcggcgc gcgaggcgcc
     3061 ggggtgagcg tctgcattct cgacagcggc atcgacgcga cccacccgcg cgtcggcggc
     3121 gtcgagagcg ccaaggctat cgtcgtcgag gctggagagc cgctcgtcgt cgacgacgac
     3181 gagggagacg tctgcggcca cggaacggcc tgcgccggga tcgtccgaca cgtcgctccc
     3241 ggctgcgaga tccactccgt gcggattctc ggcacgaagg caacgggaag cggcgacagt
     3301 ctcctggccg gtctccgcca cgccgtcgag gagggcttcc aggtcgtgaa cctgagtctc
     3361 tcgacgacga agaagcaact tgctcagatg ctgcacgagc tcgtcgacgc cgcctatttc
     3421 cgccgcacgg tgctcgtcgc gtccgcgcac aacatgcccg tcgagagcta cccatggcgt
     3481 ttctcttccc tgatctccgt cggcagccac gagggtggag accccctgac gtggtacgcg
     3541 aacccga
//
LOCUS       DBNGNK010000203         4658 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_180363,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000203 DBNGNK010000000
VERSION     DBNGNK010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_180363"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..512
                     /locus_tag="AC150V_05985"
     CDS             288..512
                     /locus_tag="AC150V_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762508.1"
                     /translation="MTISLIFIAAGAVLAWAVSTEVSGLDITAVGVILIVVGAIGAVL
                     SLVFWSSWGGFGGGRDMVVTRDRVVERDLD"
     gene            569..913
                     /locus_tag="AC150V_05990"
     CDS             569..913
                     /locus_tag="AC150V_05990"
                     /inference="COORDINATES: protein motif:HMM:NF024857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR3762509.1"
                     /translation="MTNLPTDQSRIGAAVVLRPAGELDAYVAPELRDALREHTRDAGV
                     EVVVLDLTKVTFIDSAALGVIVGAFRTVRERDAELRIVPPSSPAARIFELTSLDRVLD
                     FYSSVELALVDC"
     gene            complement(877..3438)
                     /locus_tag="AC150V_05995"
     CDS             complement(877..3438)
                     /locus_tag="AC150V_05995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR3762510.1"
                     /translation="MTTDVSRLVDEIFSNVADSAGIAVAVCDPDGTLRRVNATFARAT
                     GRRVEELLGSAPADLGLSFPTAAYHELVAAAAATGTPIVDIETVVPEGSGVPARVWLA
                     SFHPVQAAGRVEAVGVLAFDVTESRSADQLRREREETLRLTLDETETGVWSWTVGSDE
                     IDWSPKVSTVHGLPGGTPTPRRFEDYIELVHPEDRAALSNAVERAVERGEGYELDFRV
                     VPPGGSVRWVWTSASMLETAPGRARRLVGITRDATRRVRREEQQRYFREAAGVLMSSL
                     DETGMLTELARLAVGSVCDWCSFELVRDGELENVAVAHEDPGKVELAHELRRRSPPQP
                     DAPYGVGKVLRTGEPELFELLTEDTLTLLVEDKDVLRILLDLGLHSAMIVPLSIRERT
                     IGTATFVSSESGRAYGKRDLDFALEVSRSIALAVENARLFAAERRARAAIEEAHTRTA
                     RLQRVTESLGVTITADEVVSVMTNEGREAIGARTCRLYILDGDRLLLYGRGGSDGTVD
                     DETLEVHGTSTHLAVEAIRKLRPVVAGVKKGDGSAATDDLPDTGARIGVPLLSGAAPV
                     GALLLSFPDDFEPTAGDRALAMTVGRLCAQALERALVFEREHEVAVTLQQSMLPQSLP
                     QVPGIRIAAKYLPGVEGLHVGGDWFDAVTIDGGPLAVTVGDVVGKGVFAAAAMGQLRN
                     AVRVYALEGYGPGDLLARLSERADDVSSASFATLVHVLVDPQSGSCRYAAAGHPPPLL
                     RRRDGSVVRLEGGRTTPLGAGPTAAEEGSVELGGGDTLILYSDGLVERRTLPLLAGLD
                     ALEAAVASASQEGVVPLVDDLLRALQADSREDDVVVVALRLDRDQQSTSASSTEE"
     gene            complement(3580..4008)
                     /locus_tag="AC150V_06000"
     CDS             complement(3580..4008)
                     /locus_tag="AC150V_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3762511.1"
                     /translation="MSARVVRLAFPSKAEYLILARLAIAGIGRAISLDEEGVADLKLA
                     VTEACANAVKHAYPGGVGEIRVRYDIGKESLVVTVEDDGVGFDATSLDDSGQREEMTE
                     GMGIPLIRAIADDVAVTAGEARGTVVRFSKTLAHDAPSLV"
     gene            complement(4005..>4658)
                     /locus_tag="AC150V_06005"
     CDS             complement(4005..>4658)
                     /locus_tag="AC150V_06005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR3762512.1"
                     /translation="RSLAGRYAGRGEPFEDLVQVGTVGLLLAIDRFEPERGVRFTTYA
                     VPTVVGEIQRHFRDKAWMLHVPRGVKDLSVRVSRLVDDLSAQTGWTPTVSELATAAGA
                     AEEEIIEALEAGSALSVASLSQPVGEAGDDGATLEDALGEIDRGFGQTEDELTVATGL
                     SGLDARERRIVELRFRDELSQAEIAAEVGISQMHVSRLIRQSLATMRGRIEGEGTSQ"
BASE COUNT          708 a         1683 c         1527 g          740 t
ORIGIN      
        1 aggcctggtt gacgggtcag aaccgccgtc gcgtctgggt ctgcgcgccg ccggtctcct
       61 acaccctcac gctggtcgcg caaggctcgc cgggcgagcg gctggaggtc gagcgcggca
      121 ccggctgccg gtgaccggaa cccacgcgtt cgccggcccg actctcgacg gtcacgtgtc
      181 tgacgtgcgt cccccggctc gcaccgcccg cgtttcggcg gtccgcgagg cgggtaaccc
      241 tagttcctga acgtgaacac ggaggcaaac aggaggccgt catgggcatg accataagtc
      301 tgatcttcat cgccgccggg gcggttctgg cgtgggccgt ttccaccgag gtctccggcc
      361 tggacatcac cgcagtcggc gtcatcctga tcgtggtcgg cgcgatcggc gccgtgctct
      421 ccctcgtctt ctggtcgtcc tggggcgggt tcggaggcgg ccgcgacatg gttgtcacgc
      481 gtgaccgtgt ggtcgagcgc gaccttgact gagcaaacag gctgtctgct ggggcccggc
      541 cgggaaaccg ggccctagtc ttcgggccgt gaccaacctc cctaccgatc agtcgcgcat
      601 aggtgcagcc gtcgtgctcc gcccggcggg agagctagac gcgtacgtcg cccccgagct
      661 gcgcgacgca ttgcgcgagc acacccgcga cgccggcgtc gaggttgtcg tactcgacct
      721 cacgaaggtc acgttcatcg actccgccgc actcggcgtc atcgtcggcg cgtttcgtac
      781 ggtgcgtgag cgggacgccg agcttcgcat cgtgccgccg tcgtcgccgg cggcacgaat
      841 cttcgagctc acgtctctcg accgcgtcct cgacttctac tcttccgtcg agctcgcact
      901 cgtcgactgc tgatcgcgat cgagccgaag ggcgacgacg acgacgtcat cctcacgcga
      961 gtccgcctga agcgctctca ggagatcgtc gacgagcggg acgactcctt cctgggacgc
     1021 agaggcaacg gcggcctcca gagcgtccag ccccgcaagg agcgggagcg tgcgccgctc
     1081 gacgaggccg tccgaataga ggatcagggt gtcgccgcct ccgagctcca cggatccctc
     1141 ctccgccgcc gtcgggccgg ccccgagcgg cgtcgtccgc ccaccctcca gtcgcactac
     1201 cgagccgtct cggcgacgca gcagcggagg gggatggccg gccgcggcgt aacggcagct
     1261 acccgactgc gggtcgacga ggacgtggac gagggtggca aacgacgcgc tggaaacgtc
     1321 gtccgcccgt tctgagagtc gcgccagaag atcgcccggc ccgtaaccct cgagggcgta
     1381 gacgcgcacg gcattccgca gctgtcccat cgccgcggcg gcgaacactc ccttgccgac
     1441 gacatcgccg accgttacag ccagcggccc gccgtcgatc gtcactgcgt cgaaccagtc
     1501 tccacctacg tgcagtccct cgacgccggg aaggtacttg gccgcgattc gaatccccgg
     1561 gacctgcggc aggctctggg gaagcatcga ttgctgcaac gtcaccgcga cttcgtgctc
     1621 gcgctcgaac accagtgccc gctcgagggc ttgtgcgcac agcctgccga cggtcatcgc
     1681 cagtgcccgg tcccctgcag ttggctcgaa gtcgtccggg aaggacagca ggagagctcc
     1741 caccggcgcc gcgccggaca ggaggggcac gccgattcgg gcgccggtgt ccggaaggtc
     1801 gtccgtcgca gctgatccgt cgcctttctt gaccccggcg acgaccggcc gtagcttcct
     1861 gatcgcctcg acagcgagat gagtcgacgt tccgtgaacc tcgagtgtct cgtcgtcgac
     1921 ggtgccgtcc gatccgccgc gtccgtagag cagcaatcga tcgccgtcga ggatgtagag
     1981 acggcacgtt cgcgccccga tcgcctcgcg gccctcgttg gtcatcaccg acacgacctc
     2041 gtccgccgtg atcgtcacgc ccagcgattc ggttacccgc tgaagacggg ccgttcgagt
     2101 atgggcctcc tcgattgcgg cgcgcgcccg tcgctccgct gcgaacaggc gcgcgttctc
     2161 gacggcgagc gcgatcgacc gcgacacctc gagggcgaag tcgaggtccc tcttcccgta
     2221 ggcgcgaccc gactccgagc tgacgaacgt cgccgtgccg atcgtccgct cgcggatcga
     2281 gagcgggacg atcatcgcag agtgaagccc gaggtcgagc aggatccgaa ggacgtcttt
     2341 gtcctcgacg agcagtgtga gcgtgtcttc cgtcaggagc tcgaagagct ccggctctcc
     2401 cgtacggaga accttgccga cgccgtacgg cgcgtcgggc tggggcgggc tgcgacggcg
     2461 gagctcgtgc gcgagctcga ccttccccgg atcttcgtgg gcgactgcga cgttctccag
     2521 ctcgccgtcg cggacgagct cgaacgagca ccagtcgcag acgctcccca ccgcaagccg
     2581 tgcgagctcg gtgagcatcc cggtctcgtc gagcgacgac atgagcactc cggccgcctc
     2641 gcggaaatag cgctgctgct cctctctgcg gacgcgccgc gttgcgtcac gcgtgatccc
     2701 gacgagccgg cgcgctcggc cgggcgcagt ctcgagcatg ctcgcactcg tccaaaccca
     2761 ccgcaccgag cctcccggag ggacgacgcg aaagtcgagc tcgtagccct cgccgcgctc
     2821 gaccgcccgc tcgacggcgt tgctcagagc ggcgcgatcc tcggggtgga cgagctcgat
     2881 gtagtcctcg aaccgccgcg gcgtcggggt gccgccgggg agaccgtgca cggtggagac
     2941 cttcggcgac cagtcgatct catccgagcc caccgtccac gaccagacgc ccgtctcggt
     3001 ctcgtcgagc gtcagccgca gcgtctcctc acgctcacgc cgcagctggt cggcggagcg
     3061 ggactcggtg acgtcgaagg cgagcacgcc cacggcttcg acccgtccgg ctgcctgcac
     3121 ggggtgaaag ctcgcgagcc acacacgcgc cggcacgccg ctcccctcgg gcacgacagt
     3181 ctcgatgtcg acgatcgggg ttcccgtcgc ggcggccgcg gcaacgagct cgtggtaggc
     3241 agccgtggga aacgagagac cgagatctgc cggcgcagat ccgagcagct cttccacgcg
     3301 gcgaccggtc gcccgggcaa aagtggcgtt caccctccgc aatgtcccgt cgggatcgca
     3361 gactgcgacc gcgattcccg cgctgtcggc gacgttcgag aagatctcgt ccacgagccg
     3421 tgacacgtcg gtcgtcacgc tcgatcgcct cgcctcgtcc tgcaacggcc cggagctgtc
     3481 gtcacgacgc tgggcgtgac ctgggcgacg atgggcgggg ttcgagtctc gacgatgcgg
     3541 gcatcgtcca ccatagcctt tggcggtggg ccgaagtcct tacacaaggg acggagcgtc
     3601 gtgagcaagg gtcttgctga agcgcaccac agtgccccgg gcctcgccgg ccgtcacggc
     3661 gacgtcgtcg gcgatcgcgc ggatcaacgg gatacccatc ccctcggtca tctcctccct
     3721 ctgcccggag tcgtccaggg acgtggcgtc gaacccgact ccgtcgtcct cgacggtgac
     3781 gacgagcgac tccttgccga tgtcgtacct cacacggatt tcgccgactc cgcccgggta
     3841 cgcgtgcttg acggcgtttg cgcacgcctc cgtgactgcc agcttgagat ccgccactcc
     3901 ctcttcgtcc aacgagatcg ctcgtccgat ccccgcgatc gcgagccggg caagaatcag
     3961 gtactcagcc ttcgacggaa acgcgagcct gacaactcgg gcgctcactg cgacgtgccc
     4021 tccccctcga ttcggccgcg catcgtggcg agggactggc ggatcagcct cgagacgtgc
     4081 atctgcgaga tcccaacctc ggctgcgatc tcggcctgcg acagctcgtc gcggaagcga
     4141 agctcgacga tgcgtcgctc gcgggcgtcg agccccgaga gacccgttgc aaccgtcagc
     4201 tcgtcctctg tctgtccgaa tccccggtcg atctcgccga gggcgtcctc gagcgtcgcg
     4261 ccgtcgtccc ccgcttcgcc gaccggctgc gagagcgatg ccacggagag ggcggagcct
     4321 gcctccagcg cctcgatgat ctcttcctcc gccgcgcccg ctgccgtcgc gagctcggaa
     4381 acggtcggcg tccacccggt ctgcgcggag aggtcgtcca cgagccgcga gacgcggacg
     4441 ctgagatcct tgaccccacg cgggacgtgg agcatccagg ccttgtcgcg gaaatgccgc
     4501 tggatctcgc cgacgaccgt cggcaccgcg tacgtcgtga accgaacgcc ccgctccggc
     4561 tcgaaccggt cgatcgcgag caggagcccc accgtcccga cctgaacgag atcctcgaac
     4621 ggctcgcccc ttcccgcata ccggccggcg aggctgcg
//
LOCUS       DBNGNK010000204        12448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1756875,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000204 DBNGNK010000000
VERSION     DBNGNK010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 12448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12448
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1756875"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1305)
                     /locus_tag="AC150V_06010"
     CDS             complement(16..1305)
                     /locus_tag="AC150V_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762513.1"
                     /translation="MLVRGGLAVLALLVAVLFVTAGSAATEDETLYTLTVRFTGTAVV
                     SGAAEDTSLDSRRTTTYVAQSVRPFAFTRSGSTFRFSTQLRGSLSHIGLTKSTNGPCT
                     SIYFETDRVKRVEGLVWLDGRDRAPLDVDVNWVTGGAKPVQTEVRASCSPPVFPSYES
                     STLVFRIPSEFQLEKVDWAKRYGKAFTLTHSEQLGPRSPGKHLCAGGPFSDATCSVRY
                     EWTLAFTPVEQTKSERWQVDVSGSDRWTLGVVPGVGAGVWVDWTHRTVLELRGGKITS
                     AKGSVAVTRVTPVSEPAGVFAVTSRWKPRPAYRLRTASKRGKVVTLALWPHRNAAYRV
                     DFTIRLAGPQALARLRAAGAPNPDSVYKRLAERGPIALFEVSPVPFRPRLVVTLRPRA
                     SSRYTDAFTTELSCPRQRDLDCFRRRGGETVTVTKLS"
     gene            1550..3826
                     /locus_tag="AC150V_06015"
     CDS             1550..3826
                     /locus_tag="AC150V_06015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3762514.1"
                     /translation="MTTIQEPRTKTLVRPDGHEKVTGTGRYTADLTRTGLLHAKFRYA
                     DHTHARILRIDTARARALPGVIAVVTHEDVPDVLYGGMVKDRRLFARDTVRFEGDVVA
                     GVAATTPEIASAAAALIEVEYEPLPAETDFEATMADGAALIHPDWESYEGDEALGRGG
                     NVLGHSSIVSGDADAAMAGADVVVKGRYVTDPVQGVPIEPRAIVAEWQGNRVTVWTST
                     QVPYVARAGVAETLQIPESDARVVVPLLGGGFGAKCDFHFEGHVAAIARAAGQPVKLV
                     FSRREEFVAVGHRREGMVIELETGARKDGTLVARRARLVLDKGAYCGEGGFFAQMAAM
                     HALGPYEIETAHIDSYLNYSNNQPSSSIRAPTAPQVCWALEQHMDELAAALELDPVEL
                     RRRTLIEEGSVTATGQVLEQVAMKETLERAVEMIDYGRELPADEAIGVACGWWPCFAA
                     NAGAYVKLNPDGTGTIVTGAQENGTGAVMAMPAFVAEELGMRPEDFSLLYQDTDAAPW
                     DMGSCGSQTTFNSARAVIAAAGEVREQLLDAGAEKLEAAREDLELVGGSVRVKGSPDK
                     VVTIAELAGDGTFHGKGAGAVPEAPAAPAEGCAGRLGLESFHAPQLIAHAVHLKVDRE
                     TGVVRVLRVAAAHDCGVILNESGANGQVYGGVVMGLGQALTEGTQLDEEGRHRNAHLT
                     DYKLVTASDTPAIDIAWIETNTANAGPKGSKGVGEPPCVPTAGAVANAIAKVIGTRVR
                     ELPMTAERVWAARRGAGS"
     gene            3823..4689
                     /locus_tag="AC150V_06020"
     CDS             3823..4689
                     /locus_tag="AC150V_06020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3762515.1"
                     /translation="MSVSSFVTATTVDEAVAAIAGGARAIAGGTDLVVADRTGKSTLP
                     ESLVAIHRVAELGGVETSGQGIRIGALATHADLVASDEVRTRFTALADACAIVGSHAT
                     RAQGTVGGNLMNASPAMETGGPLLCFDASVTLHSPSGTRRLTLDDLWSGPGETTAQPD
                     ELLVAVDVPAPAAGTVSAYVRLEYRRQMEIAVVGATAVLTLEGGNVGDARVAMTALSP
                     TIRRVPAAEAALVDSDGGTAAVEAAGAAVAEAAEPISDVRGSGDYRRAMAGVITRRAI
                     ELALARAREERA"
     gene            4686..5153
                     /locus_tag="AC150V_06025"
     CDS             4686..5153
                     /locus_tag="AC150V_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3762516.1"
                     /translation="MKMAATLEVNSVSYPVELDPATTLLAAVRDILGLTGAKEGCDDS
                     ECGACMMLLDGRPVNACSYLALQAEGAEITTVEGLSNGAELAPLQAAFLEHGGVQCGF
                     CTPGMLVSATALLATNPHPSEEDVRIGLSGNLCRCTGYDGIVKAVLAVAEGSR"
     gene            5234..6745
                     /locus_tag="AC150V_06030"
     CDS             5234..6745
                     /locus_tag="AC150V_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762517.1"
                     /translation="MRVAAAAVLAGGLIALAGLAGSVTTPAHALGAERPVKDVTLYVA
                     DLRKAARLAKSAVGSTGALASSGSQACARVEPAATSLAAAAAAAEAAFRRYQNAKTRY
                     GTLEEIELLGSLSADLLAARKSLAKAAGGGSAPSAAEQAAAERQVSLFQTLVANKVSD
                     RLQIEGLRDVLLSTSFREAKETVAAEVGRRIKTRAEAELRRLVGLRIKLDVPLKVQIN
                     DFLERELSRFLSKLVVSAGPAGIIISIVAGRLFNPGQLVRIVGAKLKEALRPKGNLDR
                     RTRRSTAGLEQLRRDLNALTPDSPLNKVVSAAGRAQRELGRTKFLEGDLKRAGRTELL
                     QGLTSAKKNLERTIRLARARFLLDSERVDFDFRAAIAQSKRAAAEAVAIAKKLGCDEA
                     EPPRPAGDACKENWWSGTWNRIEHGGQAMFTQGGRSISSGSYTWFGGGKFAGMKLSSD
                     CKTMTGTSPKTKQLKAAVFAIELQSRDLFTGKWRWATTPAGSWNGNFGGRKVP"
     gene            complement(6742..7368)
                     /locus_tag="AC150V_06035"
     CDS             complement(6742..7368)
                     /locus_tag="AC150V_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762518.1"
                     /translation="MTVFNHQVVDADTSVAYEAIRQLDLVEVMQASPFLRVLVAARGV
                     PESLFARARSRRPPELPNTLPLAGSGQGVPGWGLLGEQPGRELVVGAIGRFWTPQIEW
                     LEIPPEEFASFEQPGYGKIAWSFSVAPYGESRSLVTNECRTKTTDPRSRAHFLRYWRL
                     VRPFAGYVMACSLPVVKAHAEATSRRSISESGSPTAPGEPDGSQGTTS"
     gene            7652..8167
                     /locus_tag="AC150V_06040"
     CDS             7652..8167
                     /locus_tag="AC150V_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR3762519.1"
                     /translation="MRSIVVCESWFGNTERVARAITDLLARHGEAELVSVEDEIDELS
                     SYDLVVVGGPTHAHGMSSATTRRGAIEQAGHDGGEAGPGVRGWLKELPKARGGHAAVF
                     DTRIEKSVVLVGSAAKGIGKRLKRHGFELVAPPESFFVLGTDGPLKEGELERAAAWAD
                     MLVARVRQPVG"
     gene            8209..8523
                     /locus_tag="AC150V_06045"
     CDS             8209..8523
                     /locus_tag="AC150V_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HIR3762520.1"
                     /translation="MTEMRLFDLRVTVESIEGRSVCGLQVGDWFELTESSRVRLPEGR
                     HFCLYAMSAVFPLLPAKQRRLPAEDWLEQDSLVTCPDPDERVVMRIERIGERTLRADE
                     LT"
     gene            8554..9219
                     /locus_tag="AC150V_06050"
     CDS             8554..9219
                     /locus_tag="AC150V_06050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR3762521.1"
                     /translation="MSDVNPDPADRRPVRFLVFSASLRAASLNSRLAALAAETLEAQG
                     GEVDHPSMREFEMPPYDADVEKAEGLPSGAQALLERLSASDAFVIASPEYNASMPGLL
                     KNAIDWVSRARPQPFHERHGLLLSASPSMVGGNRGLWALRIPFEHLGARVFPDMFSLA
                     QAHAALDDRGRISDERLQERFDATIAGFVDLVEAAKHYPCVKTKWVEFLGERPEPVLD
                     RVE"
     gene            complement(9221..10147)
                     /locus_tag="AC150V_06055"
     CDS             complement(9221..10147)
                     /locus_tag="AC150V_06055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR3762522.1"
                     /translation="MKIAVVGAGAMGSVYAGLLADAGNEVWAVDTWEEHVAAISRDGL
                     RVEGASGDRTVRLRATTRAEDVGSVDLVVIATKAMGVRAAAASARDLLGDETLVLPIQ
                     NGIGSVEAVADVLGDDRIIVGVAGGFGASVVAPGHVHHHGLELVRLGERDGPVTERVE
                     RVADVWRNAGFRVQTYDDVARLVWEKLICNVCFSGPCAVLDATIAELMASERGWVVGT
                     RCAVEAYEVAVTLGVGLDIDDPIEHVRAFGAAIPGARPSVLLDLRAGRPSEIDVINGA
                     IPRVAHTVGKTAPVNDTVAALVKALEERRVSG"
     gene            complement(10170..10586)
                     /locus_tag="AC150V_06060"
     CDS             complement(10170..10586)
                     /locus_tag="AC150V_06060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3762523.1"
                     /translation="MADVDTVVHDALTVAAVGEALVGSGALVLNVAEQMPVYTTGSWR
                     PFDGQEDAFVAAWREFMAWAAEFPGAGEIRLARDLRDPARFVSFAVWDDIEAVRVWKG
                     SPEFKERMSRVQRHIDAFAPTELDVVETLDPGAPAQ"
     gene            10560..11045
                     /locus_tag="AC150V_06065"
     CDS             10560..11045
                     /locus_tag="AC150V_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3762524.1"
                     /translation="MDDRVDIGHVHLKVADLDRAIAFYNGVLGFEVTQRLGDQAAFLS
                     AGGYHHHIGLNTWESRGGSPPDRGTTGLYHFASRYPTRAALADALMRLLAANVRLDGA
                     SDHGVSEAIYLRDPDHNGLELYWDRPRSEWPHGPDGRLAMVTEPLDLRDLLAEVEARA
                     T"
     gene            complement(11062..11313)
                     /locus_tag="AC150V_06070"
     CDS             complement(11062..11313)
                     /locus_tag="AC150V_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762525.1"
                     /translation="MEEAGIPIRVFLGDGTDLGIAHVPPPVAPGDLFALEEGLPLRVV
                     KVAPTPKHSRFHALVEVLYERRDLFRRGGQNTVPRPPAL"
     gene            11451..12203
                     /locus_tag="AC150V_06075"
     CDS             11451..12203
                     /locus_tag="AC150V_06075"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762526.1"
                     /translation="MSGAEHRRTARLTGKRAFVTGGGSGIGAAIASRFRREGAEVVVG
                     DLQGGDVHCDVRSRESVEAAIALSTERLGGLDTLALNAGRPIVGPLHELDEADWDDGI
                     ATNLTSLFRCTKAAWPHLTRDGGTITITGSVVGLWGSQNQAAYSASKAAAVMLAKCIA
                     LDGAKAGVRANCVCPGFVETPLLERFLADQEDPEAVRHGATALHPLGRLGQPADVAEA
                     FVYLASDESAWVTGATLTVDGGLTAGIWSGSA"
     gene            complement(12200..>12448)
                     /locus_tag="AC150V_06080"
     CDS             complement(12200..>12448)
                     /locus_tag="AC150V_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762527.1"
                     /translation="GGATFANAGKRVEYASRRNRGEIVALEDGSEWEVAESQRSRTAP
                     WRAGAHKVVVFQGDGPWPHICAMINLDRGEVIDVRRVT"
BASE COUNT         1960 a         4347 c         4354 g         1787 t
ORIGIN      
        1 gcatgaggcg cgccctcagc tgagcttggt caccgtgact gtctcgccgc cgcgccggcg
       61 gaagcagtcc agatcgcgct gccgcggaca cgacagctcc gtggtgaagg cgtccgtgta
      121 gcgggaggat gcccgcgggc ggagcgtcac aacgaggcgc ggccggaagg gcaccggcga
      181 gacctcgaag agagcaatcg gcccgcgttc agcgaggcgt ttgtaaacgc tgtccgggtt
      241 cggcgccccg gcggcgcgca gccgcgccag tgcctgcggc cctgcgagcc ggatcgtgaa
      301 gtccacccgg taggcggcgt tgcggtgcgg ccagagggcg agtgtgacga ccttgccgcg
      361 cttcgaggct gtcctcagcc ggtaggcggg gcgcggcttc cagcggctcg tgaccgcgaa
      421 gaccccggcc ggctcggaga ccggcgtgac cctggtgacg gcgacgcttc ctttcgccga
      481 ggtgatcttg ccgcccctga gctccagcac ggtgcggtgc gtccagtcca cccagacgcc
      541 ggcgccgacg cccgggacga cgccgagcgt ccagcggtcg ctgccgctga cgtccacctg
      601 ccagcgctcg ctcttcgtct gctccaccgg cgtgaacgcg agcgtccact cgtagcgcac
      661 gctgcacgtg gcatcgctga acggcccgcc ggcacaaaga tgcttgcccg ggctcctcgg
      721 gccgagctgc tcgctgtgcg tgagcgtgaa cgcttttccg taccgcttcg cccagtcgac
      781 cttttccagc tggaactcgg acgggatcct gaagacgagg gttgagctct cgtagctcgg
      841 aaacaccggc ggagagcacg aagcccgaac ctctgtctgg accggcttcg cgccgcccgt
      901 cacccagttg acgtcgacat ccagcggtgc gcgatcgcgg ccgtcgagcc agacgagccc
      961 ttccacgcgc ttgacacggt ctgtctcgaa gtagatcgac gtgcacggcc cgttcgtgct
     1021 cttggtgagc cctatgtggc tgaggctgcc gcggagctgg gtgctgaagc ggaaggtcga
     1081 tccgctgcgc gtgaacgcga acgggcgcac gctctgcgcg acgtacgtcg tcgtgcgacg
     1141 cgaatccagc gaagtgtcct ccgcggcacc gctcacgacg gcggttcccg tgaaccgcac
     1201 cgtcagcgtg tagagggtct cgtcctccgt cgccgcggag ccggccgtga cgaagagcac
     1261 cgcgacgaga agggccagga cggccaatcc gccccgaacg agcaaccggc cagagcgcct
     1321 gtctccatgc ctcgggcgcc gtgtcctgcg cccgccacac agtgtcgcca tcggcagcct
     1381 ccccgctccg cgccctcgtc gcactcacga gcgacgggga cacttccaag ctgccacacg
     1441 ggccgggccc tgtcttccgc ggatccaccg atccgcagtg aggggttcca ggaagcggcc
     1501 ctcctctgat tggatgcggg ctacacgacc acgctgagga gcccccaccg tgacgacgat
     1561 ccaggaacca cgcacgaaga cgctcgtccg gccggacgga cacgagaaag tcacgggcac
     1621 cgggcggtat acggccgacc tcacgcggac cggcctgctg cacgcgaagt tccggtacgc
     1681 ggatcacacg cacgcgcgga tcctgcgcat cgacaccgca agggcgcgcg cgttgccggg
     1741 agtgatcgcg gtcgtcacgc acgaggacgt gcccgacgtc ctctacggag ggatggtaaa
     1801 ggaccgccgc ctcttcgcac gtgacacggt gcgcttcgaa ggggacgtcg tcgcgggtgt
     1861 cgcggcgacg accccggaga tcgcgagcgc tgcggcggcg ctgatcgagg tggagtacga
     1921 gcccctgccg gccgagacag atttcgaagc gacgatggcg gacggcgcag cgctgatcca
     1981 ccccgactgg gagtcgtacg agggcgacga agcgctcggg cgcggcggca acgtgctcgg
     2041 gcactcgtcg atcgtcagcg gcgatgccga cgccgcgatg gctggcgccg acgtcgtcgt
     2101 gaagggccgc tacgtcaccg atccggtgca gggagtgccg atcgagccgc gcgcgatcgt
     2161 cgccgagtgg caggggaatc gcgtgacggt ttggacgtcg acacaggttc cgtacgtcgc
     2221 ccgtgccggc gtcgccgaga cgctgcagat cccggagtcg gacgcccgcg tcgtcgtgcc
     2281 gctgctcggc ggcggtttcg gcgcgaaatg cgacttccac ttcgaaggcc acgtggccgc
     2341 aattgcccgc gcggcaggac aaccggtcaa gctcgtcttc tcgcgccgcg aggagttcgt
     2401 cgccgtcggc caccgccgcg agggcatggt gatcgagctc gagacgggcg cccgcaagga
     2461 cgggacgctc gtcgcccggc gcgcgcggct cgtgctcgac aagggtgcgt actgcggcga
     2521 aggcggcttc ttcgcgcaga tggcggccat gcacgcgctc gggccgtacg agatcgagac
     2581 cgcccacatc gactcgtacc tgaactactc gaacaaccag ccctcctcgt cgatccgcgc
     2641 tccgaccgcg ccgcaggtgt gctgggcgct cgagcagcac atggacgagc tcgccgccgc
     2701 gctcgagctg gacccggtcg agctacgtcg ccgcacgctg atcgaggagg gctcggtgac
     2761 ggcgaccggc caggtgctcg agcaggtggc gatgaaggag acgctggagc gcgcggtcga
     2821 gatgatcgac tacgggcgcg agctgccggc ggacgaggcg atcggcgtgg cgtgcggctg
     2881 gtggccctgc ttcgccgcga atgcgggcgc gtacgtgaag ctgaacccag acggcaccgg
     2941 gacgatcgtg acgggtgccc aggagaacgg aacgggagcg gtgatggcga tgcccgcgtt
     3001 cgtggccgag gagctcggca tgcggcccga ggacttctcg ctcctctatc aagacaccga
     3061 cgcggcgccg tgggacatgg gctcatgtgg gtcgcagacg accttcaaca gcgcgcgagc
     3121 ggtgatcgcg gccgcgggcg aggtccgtga gcagctcctc gacgccgggg ccgagaagct
     3181 cgaggcggcc cgggaggatc tggagcttgt cggcggctcg gtgcgggtga agggctcgcc
     3241 ggacaaggtg gtgaccatcg ccgagctcgc cggcgacggc acgttccatg gcaagggcgc
     3301 aggcgctgtg cccgaggcgc ccgccgctcc cgccgaagga tgtgcgggca ggctcggcct
     3361 cgagtccttc cacgcgccgc agctgatcgc gcacgccgtt catctgaagg tcgaccgcga
     3421 gaccggcgtc gtgcgcgtgc tccgcgtggc cgcggcacac gactgcggcg tgatcctcaa
     3481 cgagtctggc gcgaacggtc aggtgtacgg cggcgtcgtg atgggcctcg gccaggcgct
     3541 gaccgagggg acacagctcg acgaggaggg caggcatcgc aacgcgcacc tgaccgacta
     3601 caagctcgtc accgcatccg acacgccggc catcgacatc gcctggatcg agacgaacac
     3661 cgcgaacgcg gggcccaagg gctcgaaagg cgtcggcgag ccgccctgcg tcccgaccgc
     3721 cggtgcggtc gccaacgcga tcgcgaaggt gatcgggacg cgggtgcgcg agctgccgat
     3781 gacggccgag cgcgtctggg cggcacgtcg gggagcgggc tcgtgagcgt gtcgtcgttc
     3841 gtgacggcca ccacggtcga cgaggcggtc gcggcgatag cgggcggcgc ccgcgcgatc
     3901 gccggtggca ccgatctcgt ggtcgccgac cgcaccggga agtccacatt gcccgagagc
     3961 ctggtcgcga ttcaccgcgt cgccgagctg ggcggagtgg agacgtcggg ccaggggatc
     4021 cgcattggcg cgctcgccac ccacgccgac ctcgtcgcga gcgacgaggt ccgcacacgc
     4081 ttcaccgcgc tcgcggacgc ctgtgcgatc gtcgggtcgc atgcgactcg ggcgcagggg
     4141 acggtcggcg ggaacctgat gaacgcctcg cccgcgatgg agaccggcgg gccgctgctc
     4201 tgcttcgacg cgtcggtgac cctccactcg ccgtcgggaa cccgccggtt gacgctcgat
     4261 gacctctggt cggggcccgg cgagacgacg gcccagccag acgagctcct cgtggccgtc
     4321 gacgtccccg cgccggccgc gggcaccgtc agcgcctacg tgcggctcga gtaccggcgg
     4381 cagatggaga ttgctgtggt cggagcgacc gccgtcctca cgctcgaggg cggcaacgta
     4441 ggcgacgcgc gcgtcgcgat gacggcgctc tccccgacga tccggcgcgt tcccgctgcc
     4501 gaggccgccc ttgttgacag cgacggaggg actgcggccg tcgaggccgc aggcgcggcg
     4561 gtcgcggagg ccgcggagcc gatctccgac gtgcgcggct ccggcgacta ccggcgcgcg
     4621 atggcaggcg tgatcacccg acgtgcgatc gagctcgcgc tggcgcgcgc ccgcgaggag
     4681 cgagcatgaa gatggcggcg acactcgagg tgaacagcgt ctcctatccc gtcgagctcg
     4741 atcccgcgac gaccctcctc gctgccgtcc gtgacatcct ggggctgacc ggcgccaagg
     4801 agggctgcga cgactcggag tgcggcgcct gcatgatgct cctcgacggc cggcccgtga
     4861 acgcgtgctc ctatctcgct ctccaggccg agggcgcgga gatcacgacg gtggagggcc
     4921 tcagcaacgg cgccgagctc gctccgctgc aggccgcgtt cctcgagcac ggcggcgtcc
     4981 agtgcggctt ctgcaccccc ggcatgctcg tctccgcgac cgcgctcctc gcgaccaacc
     5041 ctcacccgag cgaggaagac gtgcgcatcg gcctctccgg caacctgtgc cgctgcacgg
     5101 gctacgacgg aatcgtgaag gccgtcctcg cggtcgccga gggctctcgc taggacagcg
     5161 gcacacgctg agatcagccg cgtgcgcggc cgagccggtt gccccccgtc ttgcgccgcc
     5221 gtaggatcga cctgtgcgag ttgctgcggc tgctgtcctc gcagggggtc tgatcgccct
     5281 ggctggtctt gcagggtcgg tcacgacgcc ggctcacgcg ctcggtgccg agcgccccgt
     5341 gaaggacgtg accctgtatg tcgccgacct ccgcaaggcc gcacggctcg cgaagtcggc
     5401 cgtcggctcc accggcgcgc tcgcctcgag tggatcgcaa gcgtgcgcac gggtcgagcc
     5461 cgccgccaca agcctcgccg cggcggcagc ggccgccgag gccgccttcc gccgctacca
     5521 gaacgcgaag acccgctacg gcacgctcga ggagatcgag ctgctgggat cgctttcggc
     5581 cgacctgctt gccgcccgca agagcctggc gaaagcagcg ggcggcggaa gcgctccctc
     5641 tgccgccgag caggcggccg ccgagcggca ggtgagcctc ttccagacgc tggttgcgaa
     5701 caaggtcagc gaccgtctcc agatcgaagg gctcagggac gtcctcctgt cgacgagctt
     5761 cagggaggcc aaggagaccg tcgcggcaga ggtcggcagg cggatcaaga cccgcgccga
     5821 ggccgagctc cggcggctcg tcggactgcg catcaagctc gatgtccccc tgaaggttca
     5881 gatcaacgac ttcttggaga gagagctctc gcgcttcctg tccaagctcg tcgtctcggc
     5941 cggtccggcc gggatcatca tctcgatcgt cgccggcagg ctcttcaatc cggggcaact
     6001 cgtccgaatc gtcggtgcga agctgaaaga ggcgttgcga ccgaagggaa acctcgaccg
     6061 ccggacgcgc cggtcgaccg cgggactcga gcagctgcgc cgagacctga acgctctgac
     6121 gccggacagc ccgctgaaca aggtcgtctc cgccgcgggc cgcgcgcagc gtgagctcgg
     6181 ccggacgaaa ttcctcgagg gcgacctgaa gcgcgccggc aggacggagc tcctgcaggg
     6241 cctaacgagc gcaaagaaga acttggagcg gacgatcagg ctcgcacggg cccgcttcct
     6301 cctggactcc gagcgcgtcg acttcgactt ccgcgccgcg atcgcccaga gcaagagagc
     6361 cgccgccgag gccgtcgcga tcgcgaagaa gctcggctgc gacgaggctg aaccgccgcg
     6421 cccggcaggc gacgcgtgta aggagaactg gtggtccggc acctggaatc gcatcgagca
     6481 cggcggccag gcgatgttca cgcaaggcgg ccggagcatc tcgagcggct cctacacgtg
     6541 gttcggcggg ggcaagttcg ccggcatgaa gctctcgagc gactgcaaga cgatgaccgg
     6601 cacgagcccg aagacgaagc agctgaaggc agctgtgttc gcgatcgagc tgcagagcag
     6661 ggatctgttc acgggcaagt ggcgctgggc gaccacgccc gcaggaagct ggaacggcaa
     6721 cttcggcggc agaaaggtgc cctagctcgt cgtgccctgc gagccgtccg gctccccggg
     6781 tgccgtcgga ctcccgctct cgctgatcga gcgtcgagac gtcgcctccg cgtgcgcctt
     6841 gacgacgggc aacgagcacg ccatgacgta gccggcgaac ggccggacga gccgccagta
     6901 gcgaaggaaa tgcgccctcg accgcgggtc tgtcgtcttc gtccggcact cgttggtcac
     6961 gagcgaccgc gactctccgt acggcgcaac cgagaagctc catgcgatct tcccgtagcc
     7021 cggctgctcg aaggaggcga actcctccgg cgggatctcg agccattcga tctgcggcgt
     7081 ccagaatcgg ccgatcgctc cgaccaccag ttcgcgcccc ggctgctcgc ccagcaaccc
     7141 ccagcccggc acgccctggc cggatcccgc caacggaagg gtgtttggaa gctcaggcgg
     7201 tctgcgcgag cgagcgcggg cgaagagcga ctcggggaca ccgcgggcgg cgacgagcac
     7261 acgcagaaaa ggagacgcct gcatcacttc caccaggtcg agttgccgta tcgcctcgta
     7321 cgcgacggag gtgtctgcgt cgacaacctg gtggttgaac accgtcacgt cgaagctcgg
     7381 taagagctgc tcgatcagga gtgggttcgt ctccgccaga atcgcctcct cgggcctgat
     7441 ctcgattcag ccggacagcg caggcgctgt catcagagga agcacgacga atcctgcggc
     7501 aagaccgcgc cttccagagg tcggctgggg gatcagcgag tcccctagcg aactgcggtc
     7561 gagcgcgttc gcgatttcag tgtcggaccg gatgcgcccg gctgcgatca gagcgaagct
     7621 gggctcaagg acgaacaagg aggtgtttcc catgagaagc atcgtcgtct gtgagtcgtg
     7681 gttcggcaac acggagaggg tggcccgggc gatcaccgat ctgctcgccc gtcacggcga
     7741 ggccgagctc gtttcggtcg aggacgagat cgacgagctg agctcctacg acctggtcgt
     7801 cgtgggcggg ccgactcacg cccacggcat gtcgtcggcg acgacgcgcc ggggggcgat
     7861 cgagcaggcg ggacacgacg gtggcgaggc cggcccgggc gtgcgtggct ggctgaagga
     7921 gctgcccaag gcccggggcg gccatgcagc tgtgttcgac acacgtatcg agaagtccgt
     7981 cgtgctcgtc ggatcggcgg ccaaaggaat cgggaaacgg ttgaagcggc acgggttcga
     8041 gctcgtggct ccaccggaga gcttcttcgt gctcggcacg gacggcccgc tgaaggaggg
     8101 cgagctcgag cgagccgccg cctgggcgga catgctggtg gcgcgcgtcc gacagcccgt
     8161 cggctgaggc caccgcgatc ggcaccgggc tctgcgaaca ttcggcccat gaccgaaatg
     8221 aggctcttcg acctacgggt caccgtcgag tccatcgagg gccgctcggt gtgcggcctg
     8281 caggtcggtg actggttcga gttgacggag agcagccgag tccggcttcc ggagggacgc
     8341 cacttctgcc tctacgcgat gtcggccgtg tttccgctgc ttcccgcgaa acagcggcga
     8401 ctacccgctg aggactggct cgagcaggac agtctcgtca cctgccccga tccggacgag
     8461 cgcgtcgtca tgcggatcga gcggatcggc gagcgaacac tccgcgccga cgagctgacc
     8521 tgagcgcgcc gggcgccgat agggtggttg tcggtgtctg acgtcaaccc cgacccggcg
     8581 gaccgccgac ccgttcgctt cctggtcttc tcggcgtcac tccgtgcggc ctccctcaac
     8641 tcgcgcctcg cggcgctcgc ggccgagacg ctcgaggcgc agggtggaga ggtcgaccac
     8701 ccgtcgatgc gcgagttcga gatgccgccg tacgacgccg atgtagagaa ggcggagggt
     8761 ctcccgtcgg gagcgcaggc attgctcgaa cggttgtcgg cgagcgacgc cttcgtgatc
     8821 gcttcacccg agtacaacgc ctcgatgccc gggctcctga agaacgccat cgactgggtc
     8881 tcgcgggcgc gcccgcagcc gtttcacgag cggcacggac tgctgctgtc cgcgtcgccg
     8941 tcgatggtcg gaggcaaccg aggactatgg gccttgcgga ttccattcga gcacctcggc
     9001 gcccgcgtgt ttccggacat gttctcgctc gcccaggccc acgctgcgct cgacgaccgc
     9061 ggacgcatct cggacgagcg gctgcaggag cgcttcgacg ccacgatcgc cggcttcgtc
     9121 gacctcgtcg aggccgccaa gcactacccg tgcgtcaaga ccaagtgggt cgagttcctc
     9181 ggcgagcgcc ccgagccggt tctcgaccgg gtcgagtagc tcagccggag acgcggcgct
     9241 cctcgagcgc tttcacgagg gctgcgacgg tgtcgttcac cggcgccgtc ttcccgaccg
     9301 tgtgcgcgac acgggggatg gcgccgttga tcacgtcgat ctccgacggc cgccccgcgc
     9361 gcaggtcgag cagcacggag ggccgcgcgc ccgggatcgc cgctccgaag gcgcggacgt
     9421 gctcgatcgg gtcgtcgatg tcgagcccga ccccgagggt cacggcgacc tcgtatgcct
     9481 cgaccgcgca acgcgtgccc actacccacc cgcgctcgct cgccatcagc tccgcgatcg
     9541 tcgcgtcgag caccgcacac ggcccgctga agcagacgtt gcagatgagc ttctcccaca
     9601 ccagtcgcgc cacgtcgtca taggtctgca cccggaaccc cgcgttgcgc cagacatctg
     9661 cgacgcgctc gacccgctcc gtgaccggcc cgtcgcgctc gccgagccgc acgagctcca
     9721 ggccgtggtg atgcacgtgt cccggagcga cgaccgaggc gccgaagccg ccggcgaccc
     9781 cgacgatgat gcgatcgtcg cccagcacgt ccgcaaccgc ttccacgctg ccgattccgt
     9841 tctggatcgg cagcaccaac gtctcgtctc cgagcagatc gcgcgccgac gccgccgcgg
     9901 cgcgaacacc catcgccttg gtggcaatca ccaccaggtc gacggatccg acgtcctcgg
     9961 cccgagtcgt tgcccgcagc cgcaccgttc gatcgccgct cgcgccctcg acccgcagcc
    10021 cgtcgcggct gatggccgcg acgtgctcct cccacgtgtc gacggcccag acctcgttgc
    10081 ccgcgtcggc gaggagcccc gcgtacacag agcccatcgc gccagcgccg acgaccgcga
    10141 tcttcacgga gccgcgctgg aggcagcgct cattgggccg gagccccagg gtcgagcgtc
    10201 tcgacgacat cgagctcggt cggggcgaag gcgtcgatgt gtcgctggac gcgactcatc
    10261 cgctccttga actccggtga ccccttccag acccggacgg cctcgatgtc gtcccaaacg
    10321 gcaaagctca cgaagcgcgc cgggtcgcgg aggtcgcggg cgaggcggat ctcgccggcg
    10381 ccggggaact cagcggccca cgccatgaac tcacgccacg cggcgacgaa cgcgtcctcc
    10441 tgcccgtcga agggcctcca cgatcctgtc gtgtacacgg gcatctgctc tgcgacattc
    10501 aagacgagcg cgccactgcc gacaagagct tcgccgaccg ctgctaccgt cagcgcatcg
    10561 tggacgaccg tgtcgacatc ggccacgtgc acctcaaggt cgccgatctc gatcgagcga
    10621 tcgccttcta caacggtgtc ctcggcttcg aggtgaccca gcgtctcggc gaccaggcgg
    10681 ctttcctgtc ggcgggcggc taccaccatc acatcggcct gaacacctgg gagtcgcgcg
    10741 gcggctctcc accggaccgc ggcacgaccg gtctctacca cttcgcgagc cggtacccga
    10801 cgcgggctgc gctcgcggac gcgctcatgc gcctgctcgc ggcgaacgtc cgcctggacg
    10861 gagcaagcga tcatggcgtg agcgaggcca tctacctccg ggatcccgat cacaacggtc
    10921 tggagctcta ctgggaccgg ccgcggtcgg aatggccgca cgggcccgac gggcggctcg
    10981 cgatggtgac cgagccgctc gacctacggg acttgctcgc cgaggtcgaa gcccgtgcga
    11041 cttgagatcg gaagcctcgg gctacagcgc aggcgggcgc ggcacggtgt tctggccgcc
    11101 gcgccggaag agatcgcggc gctcgtagag aacctcgacg agcgcgtgaa agcgcgaatg
    11161 cttcggagtc ggcgcgacct tgaccacccg caggggcagg ccctcctcca gcgcgaagag
    11221 gtcgccgggc gccaccggag gcggcacgtg cgcaatgccg aggtccgtgc cgtcgccaag
    11281 gaatacccgg atcgggattc cagcctcctc catgctctca cggtacggat gaaacgggtc
    11341 tttgtcgacg ttgaccacga cttcggcagg acaccccgcg ccccacgaga tgaggccgcg
    11401 tcggagagag gccaagaagt cgaccacacg gcgcccgggc gcgcgctgcc atgagcggag
    11461 cggagcaccg ccgcacagcc aggttgaccg gcaagcgcgc cttcgtcacc ggcggcggct
    11521 cggggatcgg cgccgcgatc gcaagtcggt tccgccggga gggcgcggag gtcgtcgtcg
    11581 gtgacctcca gggcggcgat gtccactgcg acgtccgctc gcgcgagagc gtcgaggctg
    11641 cgatcgcgct gtcgacagag cgtctcggcg gactcgacac gctcgctctc aacgccggcc
    11701 ggccgatcgt cgggcccctg cacgaactgg acgaggccga ctgggacgac gggatcgcga
    11761 cgaacctgac gtcgctcttc cgctgcacca aggcggcgtg gccgcacctg acgagggacg
    11821 gcggcacgat cacgatcacc ggctccgtcg tcggtctgtg gggctcgcag aaccaggctg
    11881 cctatagcgc ctccaaggcg gcggcggtga tgctcgctaa atgcatcgcg ctcgacggcg
    11941 cgaaagcggg cgtccgggcg aactgcgtct gcccgggctt cgtggagacg ccgctgctcg
    12001 agcgctttct ggcggatcaa gaagacccag aggcggtgcg gcacggcgcc accgcactcc
    12061 acccgctcgg gcggctcggc cagcccgccg acgtcgccga ggcgttcgtc tacctggcgt
    12121 cggacgaatc ggcctgggtg acgggcgcca ccctcaccgt cgacggcggg ctcaccgcgg
    12181 gcatctggtc cggctcagcc taggtcacgc gccgcacgtc gatcacctcg ccccgatcga
    12241 ggttgatcat cgcgcagata tgcggccacg ggccgtcgcc ctggaagacg acgaccttgt
    12301 gcgcgccggc ccgccacggc gcggttctcg atcgttgcga ctcggcgact tcccactccg
    12361 agccgtcctc gagcgcgaca atctcgccgc gattccgcct ggaggcgtac tcgacccgct
    12421 tgccggcgtt cgcgaaggtc gctccgcc
//
LOCUS       DBNGNK010000205         2574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1900773,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000205 DBNGNK010000000
VERSION     DBNGNK010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1900773"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC150V_06085"
     CDS             <1..676
                     /locus_tag="AC150V_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3762528.1"
                     /translation="ERGWVTGGTGPWEALGELLAKPYGRDAIPLLAERGALSGRVVAA
                     HCVHVTEHEIELLSGHDVAVAHCPRSNALLGCGVAPLTRLREAGVRICIATDSPASTP
                     SFDMFDELRAAVMAARSSEQRPDALTAAEALELATLGGASALGMDSEVGSIAVGKRAD
                     LAILSLSGSSFLPWEDPVTAIVLGGSPDRIIATTVDGQTRYEKAARRCHELTDAARRA
                     RSRMLA"
     gene            712..1449
                     /locus_tag="AC150V_06090"
     CDS             712..1449
                     /locus_tag="AC150V_06090"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3762529.1"
                     /translation="MFFTRLRRHAKWMFVFLALVFGVGFVAFGIGAGGTGIGDIFRGQ
                     SNSSGISVSDARSATEEDPQNAEAWRDLSTALQTEGETEEAIVALERYVQLRPKDPDG
                     LRELGGLLLTQGALQRQEAQTVQLRAQLSGATSPFSPSLIVDGVDRFENKIAAVVEDQ
                     VNAELSRILSESGASYTRGVATYRRLAEVLPTDPSVQLELAQAAEQAGSTDTAVAAYE
                     RFLELAPEDPNAPIIKQQLQRLRNSAG"
     gene            1461..2240
                     /locus_tag="AC150V_06095"
     CDS             1461..2240
                     /locus_tag="AC150V_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR3762530.1"
                     /translation="MEPSAVTPPARRLRVALLIVSAGLAAALLAGCGGAEFSEGTGDE
                     QNGKQLFVEGCGACHALADAGTSGKIGPDLDDAFYQYRVDASGARGDDGQISVDKLRD
                     PDIESTVRQVVRGQIAYPIVNTPTGAPGMPADIYTGTDAEDVSTYVASVAGVQPPDRR
                     TPAQPPAPAPPPPDGGDGGADLLAVGKTVFTANCGACHTLADAGTNGAVGPNLDDLQT
                     SERAVAEIVTNGRGAMPAFSGSLSPEEIDAVAVYVSTGAGG"
     gene            complement(2247..>2574)
                     /locus_tag="AC150V_06100"
     CDS             complement(2247..>2574)
                     /locus_tag="AC150V_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR3762531.1"
                     /translation="LVVSPALNSRLKHWTSDEDQAREEAQVRLDLSIERLAAAGITAQ
                     GAVGDDDPVQAADDALRTFGADEIIVSTHPPGRSNWLEQGVVDGVRERFDVPVTHVVV
                     DLEKRG"
BASE COUNT          428 a          891 c          890 g          365 t
ORIGIN      
        1 cgagcggggc tgggtgacgg gcggaacggg gccgtgggag gcgctcggcg agctgctcgc
       61 gaagccgtac ggccgggacg cgatcccgct gctcgccgaa cgcggcgcgc tcagcggaag
      121 agtggtagca gcccactgtg tccacgtcac tgagcacgag atcgaactgc tctcggggca
      181 cgatgtcgcc gtcgcgcact gccctcgctc gaatgcgctg ctcggctgcg gtgtggcacc
      241 gctcacgcgg ctccgcgagg cgggcgtacg aatctgcatc gccacggaca gccccgcctc
      301 gacgccgtcc ttcgacatgt tcgacgagct gcgcgccgcg gtcatggccg cgagaagcag
      361 cgaacagcgg ccggatgcgc tcacggccgc cgaggcactc gagctggcga cgctcggagg
      421 agccagtgca cttggaatgg actcggaagt gggctcgatc gccgtcggga agcgggccga
      481 tctggctatc ctctccctga gcggctcgag cttcctgccg tgggaagacc ccgtcacggc
      541 gatcgttctg ggtggcagcc cagaccgcat catcgccact accgttgacg gacagaccag
      601 atacgagaaa gctgcgagga gatgtcacga gctgaccgac gccgcgcgaa gagcacgaag
      661 ccgaatgctc gcgtaagcgc ccgcacgtcg ggtcccgcga tcgaagacac catgttcttc
      721 acgcggctcc gccgtcacgc caagtggatg ttcgtcttcc tcgctctcgt cttcggggtg
      781 ggcttcgtcg cgttcggcat cggcgccggc ggaacgggca tcggcgacat cttccgcggc
      841 caaagcaact cgagcgggat ctcggtcagc gacgcgcgct ccgcgacgga ggaagacccc
      901 cagaacgcag aggcctggcg cgacctctcg acggctctcc agacggaggg tgagacggaa
      961 gaggcgatcg tcgctctcga gcgctacgtc cagctccgtc cgaaggatcc cgacggcttg
     1021 cgggagctcg gggggctgct cctgacccag ggcgccctcc agcgtcagga ggcgcagaca
     1081 gtgcagttgc gggcacagct gtcgggcgcg accagcccgt tctcgccgag cctgatcgtc
     1141 gacggggtcg accgcttcga gaacaagatc gcagcggtgg tggaagatca ggtgaacgcc
     1201 gagctgagcc ggattctcag cgagtctggc gcgtcgtaca caaggggcgt cgcaacgtat
     1261 cgaagacttg ccgaagtctt gccgaccgat ccgagcgtcc agctcgagct ggcgcaggca
     1321 gcagagcaag ccggcagcac cgacactgcg gtcgcggcct acgagcggtt cctcgagctg
     1381 gcgcccgagg atccgaacgc accgatcatc aagcagcagc tgcagcgcct acgtaactcc
     1441 gcgggttaga ctcgcgccga gtggagccga gcgccgtcac accgcctgcg cggcgcctgc
     1501 gcgtcgcctt gctgatcgtc tcggccggcc tggccgccgc gctcctggcc ggctgcggcg
     1561 gcgcggagtt ctcggaggga accggcgacg agcagaacgg caagcagctc ttcgtcgagg
     1621 gctgcggcgc ttgccacgcg ctcgcagacg cgggaacgag cggaaagatc gggcccgatc
     1681 tcgacgacgc cttctaccag tatcgcgtgg acgcgagcgg tgcccggggc gacgacggcc
     1741 agatcagtgt cgacaagctc agagatccgg atatcgagtc gacggtgcgc caggtcgtcc
     1801 gcgggcagat cgcgtacccg atcgtcaaca cgcctaccgg cgctcccggg atgccggccg
     1861 acatctacac cggcaccgac gcggaggacg tctcgaccta cgtcgcgagc gttgccggcg
     1921 tgcagccgcc cgacaggcgc acaccggcac agccgcctgc tccagctccc ccgcccccgg
     1981 acggcggcga cggcggcgcc gacctgctcg cggtcgggaa gaccgtcttc accgcgaact
     2041 gcggtgcctg tcacacgctc gcggacgcgg gcaccaacgg cgcggtcgga ccgaacctcg
     2101 acgacctcca aacctccgag agagccgtcg ctgagatcgt gacgaacggc cgcggcgcga
     2161 tgcccgcctt ctccggcagc ctgagcccgg aggagatcga cgcggtggcc gtgtacgtct
     2221 ctaccggcgc gggcggctag cgggctctac ccgcgctttt cgaggtcgac cacgacgtgg
     2281 gtcacgggca cgtcgaatcg ctcgcggacg ccgtccacga caccctgctc gagccagttc
     2341 gaccgcccgg gtggatgcgt cgacacgatg atctcgtcgg cgccgaacgt cctcagcgca
     2401 tcgtccgcgg cctgaacggg gtcgtcgtcg ccgaccgctc cctgagcggt gataccggca
     2461 gccgccagcc gctcgatgct cagatcgagc cgaacctggg cctcttcgcg cgcctgatcc
     2521 tcgtcggacg tccagtgctt gagacgggag ttgagcgccg gcgagacgac gagc
//
LOCUS       DBNGNK010000206         4860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1325892,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000206 DBNGNK010000000
VERSION     DBNGNK010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1325892"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="AC150V_06105"
     CDS             <1..211
                     /locus_tag="AC150V_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009070819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR3762532.1"
                     /translation="ALIGAVIGIVLGIVLAILLIVRVDFIELAWPIASLIVFAAAAVV
                     VGIVAAVFPARRAARLNPLEALHYE"
     gene            complement(339..1823)
                     /locus_tag="AC150V_06110"
     CDS             complement(339..1823)
                     /locus_tag="AC150V_06110"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020755546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HIR3762533.1"
                     /translation="MVSHVSAQFSATLRVRLSDDPGSFAGIAQAIGDAGGSLGAIDLV
                     RVERHHKVRDVTVAASDESHLHAIIEAASAVDGIEIEQVSDRTFLMHLGGKLEVVGKV
                     PLKTRDDLSMAYTPGVARVCQAVAESRDAAWNLTIKKNTVAVVSDGSAVLGLGDIGPE
                     AAMPVMEGKALLFKEFGGVDAFPLCLATQDVDEIVSIVKAVSPGFGGINLEDIAAPRC
                     FQIEERLRTEVDIPVFHDDQHGTAIVVLAALVNALRVVDKTLADVKIVVSGAGAAGIA
                     TVDVLLEAGARRIIGCDRAGAIYRGRPELTPVKAEYAERTNPDGEQGSADDVLAGADV
                     FIGLSAPGAISAAAVETMASEAIVFAMANPTPEVQPEAIHDSVAVVATGRSDYPNQIN
                     NVLAFPGVFRGALDVRASDITHGMKVAASHALASVITAEELSAEYIIPSVFNRDVAPR
                     IAEAVAAAAEEDGVARRSSRGSADHIPPRPAALALRRTEEGTVP"
     gene            1930..2529
                     /locus_tag="AC150V_06115"
     CDS             1930..2529
                     /locus_tag="AC150V_06115"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR3762534.1"
                     /translation="MFNRFVDWVSGAWWSYLVIFLVSAIDAFFPLVPSETIVITGGVL
                     AGSGDLNLALVILCASAGAIVGDNISYLLGTLLGERTVKRWFKSEKSQRAFGWAEHQL
                     DTRGGYLIIIARFIPGGRTAVTFSSGYIHAMRWRRFIFWDVIAGLVWGTYAAMLGYIG
                     GKQFEEQPWKGLLLAAVIAFGLAGLIELVRHLLRRRATA"
     gene            2526..2936
                     /locus_tag="AC150V_06120"
     CDS             2526..2936
                     /locus_tag="AC150V_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3762535.1"
                     /translation="MSGSAPGLTGVSELVLEVLDLERSVAFYTEELGLPLVERWEHRE
                     AAWVMAGKRTRIGLWTPQVGIAGGRGGVHVHYAIHVDDGGYEAIKARLRERGHEVEER
                     TFRNYDRSRALYVTDPDGNVVEFWTWDVSRHLDS"
     gene            2933..3685
                     /locus_tag="AC150V_06125"
     CDS             2933..3685
                     /locus_tag="AC150V_06125"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007458293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009226 - nucleotide-sugar biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3762536.1"
                     /translation="MRLHVTGASGFLGRELVRRAPHATGERVEIRDPRSVRALLQRLR
                     PELVIHTAYRQDGAEAWATTVDGAENVARAARETEARLIHLSSDLVFDGRKGRPYVEG
                     DAPCPLTEYGRAKAEAERRVATAHPAALIVRTSLIVGGPGHEPSKHELAAQNPGLTFF
                     ENEIRSPVQVGDLAQALLELAELPESGPLHVAGADAVSRAELAELARRGPVTRAPAPA
                     GRPLDCRLDSTRARALLSTELRGVRAVFAGSG"
     gene            complement(3694..4437)
                     /locus_tag="AC150V_06130"
     CDS             complement(3694..4437)
                     /locus_tag="AC150V_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3762537.1"
                     /translation="MATRIQLCGRIRATVDDDRIESSLPGHQGLLLFVYLVVNRQRAA
                     TRAELTDALWESRAPASADAGLSSLLSKLRRVVGADRLEGRGSLRLVLPPDTWVDLEA
                     ATAGIHRAEAAIAAGDWTDAWGPARVAQHVTRRGFLPGEDAAWVLEQRRRVEEMYARS
                     LELAGLASLRIGGSELDTAERAARALVEHAPFRESGYRLLMEVLAERGNPAESLLVYE
                     SLRTRLRDDLGTSPAPETQQLHRRLLLQD"
     gene            4463..4846
                     /locus_tag="AC150V_06135"
     CDS             4463..4846
                     /locus_tag="AC150V_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009488976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR3762538.1"
                     /translation="MRVIIDVVPSLLVHITSGPEHPTRASLGFLVARAAADAGHDVSI
                     FVAGDAVQLLRPEVRGALSGLGTGSLGESFDAVAGAGAKLYASGMSSLARGVSEDILR
                     EAGAEPARPERLVELTFEHDRVLTY"
BASE COUNT          706 a         1655 c         1696 g          803 t
ORIGIN      
        1 ggcgctgatc ggcgccgtga tcggaatcgt gctcggcatc gttctcgcga tcctgctgat
       61 cgtgcgggtg gacttcatcg agctcgcctg gccgatcgcg tcgctgatcg tgttcgctgc
      121 tgccgcagtc gtcgtcggga tcgtcgcggc cgtcttcccg gcgcggcgcg cggcgcggct
      181 caatccgctc gaagcgctgc actacgagta gcggctcagt cgcccaggcc cgtctgctcg
      241 tgacctggcc cgatgcgacg cgtgtcctcg ggctctgacg tgcccgaagc cacttgtcac
      301 actttcggcg caggtcggcc ctcgccgacg aagccatgtc aggggacagt cccctcttcg
      361 gtccgcctca acgcgagcgc ggcgggtcgc ggcggaatgt ggtccgccga gccacggctc
      421 gagcgccgag cgacgccgtc ctcctctgcc gcggcggcga cggcttcggc gattcgcggc
      481 gccacgtcgc ggttgaagac actggggatg atgtattcgg cgctgagctc ctccgccgtg
      541 atgacggagg cgagcgcgtg actggctgcc accttcatgc cgtgcgtgat gtcggacgca
      601 cggacgtcga gcgcgccgcg gaacacgccc gggaaggcga gcacattgtt gatctggttc
      661 gggtagtccg agcgcccggt ggcgacgacg gctaccgaat cgtgaatggc ctccggctgc
      721 acctccggtg tgggattcgc catcgcgaac acgatcgctt cggacgccat cgtctcgacc
      781 gccgctgccg agatcgcccc gggagccgac aggccgatga agacgtcggc cccggcgagc
      841 acgtcatcgg cgctgccttg ttcgccgtcg gggttcgtcc gctccgcgta ctcggccttg
      901 accggggtga gctcgggcct tccgcggtag atcgcccccg cccgatcaca gccgatgatt
      961 cgccgagcac cggcctcgag caggacgtcg accgtcgcga ttcctgccgc gccggcaccc
     1021 gagacgacga tcttcacgtc ggcgagggtc ttgtcgacga ccctgagcgc gttgaccagg
     1081 gcggcaagca cgacgattgc cgtgccgtgc tggtcgtcgt ggaagacggg gatatcgacc
     1141 tcggtgcgca ggcgttcctc gatctggaag cagcgaggcg cggcaatgtc ctccaggttg
     1201 atgccgccga agccggggga gacggccttc acgatgctga cgatctcgtc gacgtcctgc
     1261 gtcgcaaggc agagggggaa ggcatccacg ccgccgaact ccttgaagag aagcgccttc
     1321 ccctccatca cgggcatcgc ggcttcggga ccgatgtcgc ccagcccgag aacggccgag
     1381 ccgtcgctga ccaccgcgac ggtgttcttc ttgatcgtga ggttccacgc cgcgtcgcgg
     1441 ctctcggcca ccgcttggca gacgcgggcg acgccgggcg tgtaggccat cgagaggtcg
     1501 tctcgcgtct tgagtggaac ctttccgacg acctcgagct tgccgccgag atgcatcaag
     1561 aacgtccggt ccgaaacctg ctcgatctcg atgccgtcga cggcgctggc cgcctcgatg
     1621 atcgcgtgga ggtgcgattc gtccgacgct gccacggtaa cgtcacgcac cttgtggtgc
     1681 cgctcgaccc ggacgaggtc gatcgcgccc agagagccgc cggcgtcacc gatagcctgg
     1741 gcgattcccg cgaagctccc cggatcgtcc gacagccgga ctcggagagt ggcactgaac
     1801 tgcgcggaga cgtgactcac gatctgatca ctccttcgac ggcggacgcc gtggtgtcgt
     1861 ctggggtggg gaggggcttg cccggcgtgg gcccatcatc gcggctggcg gctctgcgag
     1921 gatgcagccg tgttcaaccg tttcgtcgac tgggtctccg gggcctggtg gagctatctc
     1981 gtcatcttcc tcgtgtcggc gatcgacgcc ttcttccctc tcgtcccgag cgagacgatc
     2041 gtcatcaccg gtggagtgct cgcaggctcc ggcgacctca atctggccct cgtgatcctc
     2101 tgtgcaagcg cgggcgcgat cgtcggcgac aatatctctt atctgctcgg aacccttctc
     2161 ggtgagcgga cggtgaagcg ctggttcaag agcgagaagt cacagcgggc cttcgggtgg
     2221 gccgagcacc agctcgacac acgaggcggc tacctgatca ttatcgcccg gttcatcccc
     2281 ggcggccgga ccgccgtcac cttctcgtcg ggctacatcc acgcgatgag gtggcgccgc
     2341 ttcatcttct gggacgtgat cgccggtctc gtctggggca cctacgcggc catgctcgga
     2401 tacatcggcg ggaagcagtt cgaagagcag ccctggaagg gcctccttct cgccgccgtc
     2461 attgcgttcg gcctcgccgg gttgatcgag ctcgtcaggc acctcctgcg acgccgcgcg
     2521 accgcatgag cggctctgcg cccggtctca ccggggtgag cgagctcgtc ctcgaagtgc
     2581 tcgacctcga gcggtcggtc gccttctaca cggaggagct cggactcccg ctcgtcgagc
     2641 gctgggagca ccgcgaagcc gcctgggtca tggcgggaaa gcgcactcga atcggcttgt
     2701 ggacgccgca ggtggggatt gcaggcgggc gcggcggcgt gcacgtccac tacgcgatcc
     2761 acgtggacga cgggggctac gaggcgatca aggctcgctt gcgcgagcgg ggtcacgagg
     2821 tcgaagagcg cacgttccgc aactacgacc gcagcagagc cctctacgtg accgacccgg
     2881 acggcaacgt cgtcgagttc tggacctggg acgtgtcgcg tcatctcgac tcgtgaggct
     2941 gcacgtcacg ggggcgagcg gcttcctcgg ccgtgagctc gtacggcgcg cgccccatgc
     3001 gaccggcgag cgagtggaga tccgggaccc ccgttcggtc agggctctgc tccagcggct
     3061 gcgacccgag ctcgtgatcc acaccgccta tcgccaggac ggcgctgagg cgtgggcgac
     3121 cacggtcgac ggcgcggaga acgtcgctcg cgccgcccgc gagacagagg cgcgactgat
     3181 tcatctgtcc tccgatctcg tcttcgacgg ccgcaagggg aggccctacg tcgaaggaga
     3241 cgcgccttgt cctctgacgg agtacggacg cgcgaaggca gaggccgaga ggcgggtcgc
     3301 gacggcgcac ccggcggccc tgatcgttcg cacgtcactg atcgtcggcg gcccgggaca
     3361 cgagccctcg aagcacgagc tcgccgcaca gaatccgggg ttgacgttct tcgagaacga
     3421 gatccgctcc ccggtacagg tcggcgacct ggcccaggcg ctgctcgagc tggcggagct
     3481 ccctgaatcc ggcccgttgc acgtggccgg cgcggatgcc gtctcccgtg ccgagctggc
     3541 cgagctcgcg cgcagagggc cggtgacgcg cgctccagcg ccggcgggac gtccgctcga
     3601 ctgccggctc gactcgaccc gcgcgcgcgc gctgctgagc acggagctcc ggggcgtgcg
     3661 agcggtcttc gcaggcagcg gttgagctgc cgctcagtcc tgcagcagca gccggcggtg
     3721 cagctgctgc gtttcgggtg ccggggacgt gcccaggtcg tcgcgcaggc gcgtcctgag
     3781 cgactcgtag acgagaagcg actcggctgg gttgccacgt tcggcgagca cctccatcag
     3841 caagcggtag ccgctttctc gaaacggcgc atgctcgacg agggcacgcg cggcgcgctc
     3901 cgccgtatcg agctctgacc cgccgatgcg aagactggcc agcccggcga gctccagcga
     3961 acgggcgtac atctcttcga cgcgacggcg ctgctcgagc acccaggcgg cgtcctctcc
     4021 cggcaggaag ccgcgccgtg tgacgtgctg ggcgacgcgg gcgggccccc aggcgtcggt
     4081 ccagtcgccg gcggcgatgg ccgcctcggc tcggtggatc ccggcggttg ccgcctcgag
     4141 gtcgacccat gtatcgggcg ggagcacgag gcggagactg cctctccctt cgagccgatc
     4201 ggcgccgacg actcggcgca gcttcgacag cagcgacgag agtccggcat ccgcggacgc
     4261 aggcgcccgc gactcccaga gtgcgtcggt cagctcggcc cgggtggccg cgcgctgccg
     4321 gttgacgacg aggtagacga agagaaggag gccctggtgc cccgggagtg acgattcgat
     4381 gcggtcgtcg tccaccgtgg cccggatgcg gccgcagagc tggatgcgcg tcgccaaggt
     4441 cgcgccaagg ttaggtcaag cggtgcgcgt catcatcgac gtcgtgccca gcctgctcgt
     4501 ccacatcaca tccggccccg agcatccgac cagggcctcg ctcggcttcc tcgtcgcgcg
     4561 cgcagccgcc gatgccgggc acgacgtcag catcttcgtg gctggcgacg ccgtccagct
     4621 tctgcggccg gaggtcagag gcgcgctcag cggactcggc acagggagtc tcggcgagtc
     4681 gttcgacgcc gtcgccgggg ctggtgccaa gctctacgct tccggcatgt cgagcctcgc
     4741 gcgcggggtg tccgaggaca ttctgaggga ggccggggcc gagcccgctc gaccggaacg
     4801 tctcgtcgag ctcacgttcg agcacgatcg tgttctcact tactgatcgg aggtatcgat
//
LOCUS       DBNGNK010000207         2362 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1829804,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000207 DBNGNK010000000
VERSION     DBNGNK010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1829804"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..121
                     /locus_tag="AC150V_06140"
     CDS             14..121
                     /locus_tag="AC150V_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762539.1"
                     /translation="MAGICALVLSRHPTLTPFELKSLLYLTATNTGGTE"
     gene            118..777
                     /locus_tag="AC150V_06145"
     CDS             118..777
                     /locus_tag="AC150V_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3762540.1"
                     /translation="MSQETLRAAAAVGAIGSEESYRALLQSIVEVARGIFGAKASSVF
                     LLDEETDELVFEAVSGEGEGDLLGVRFPAGTGIAGWVLMTRQPLVLEDVATDPRFARE
                     AAERTGYVPKSMMAVPLLNEERALGVLNVLDRPQEAAFTLSEMDLLSLFGNQAAIALD
                     LLLRARRARAALAGEGSAAEAAIARVAAALEDDEGRLDPTGVALLEALADVLQKRAGA
                     V"
     gene            complement(820..1005)
                     /locus_tag="AC150V_06150"
     CDS             complement(820..1005)
                     /locus_tag="AC150V_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762541.1"
                     /translation="MSDEREAVDIGEEREAVDVGEEPDVEGHMLDVGEGEKGALDVGE
                     GEKGALDDGSGLVDLGE"
     gene            1121..>2362
                     /locus_tag="AC150V_06155"
     CDS             1121..>2362
                     /locus_tag="AC150V_06155"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762542.1"
                     /translation="MGRIADRLREGFAAAHAVAANRDLRGLQLAGVGSVLGNWAYLVA
                     LLVYAYDAGGPAAVGLVSVLRMIPAAVSAPFVSVLADRLSRRGVMIAADILRAGLMLG
                     AGAVIWADGPPWVVYLIVTFSSIVATSFRPAQSALLPVLARGPEELTAANVAAAAITS
                     IGAVIGPAIGAAVLAVSSTEVVFAINSLTFLWSAALILGVREPDGTGPARGGNPFGRE
                     LVAGLAVLAGDRGMRFLTILYVAQTVVAGTLNVLIVVTAFSLLDTGESGVGLINAAIG
                     VGGIVGGAVALALVGRQRLAGDFGVGLLLFGAPLALIPVFPELAPVLALFAVVGIGNT
                     LVDVAASTLLQRTVPNDVLARAFGALQALLLGAIGVGALIAPLLVETVGNTASLVAVG
                     ALLPVVGVAALPGLRGIDRRGR"
BASE COUNT          289 a          833 c          825 g          415 t
ORIGIN      
        1 tgcggcgccg cacatcgccg gcatctgcgc gctcgtcctc tcgcgacatc cgacgctgac
       61 gccgttcgag ttgaagtcgc tgctctatct cactgcaacg aacaccggag gaacggaatg
      121 agtcaggaga cacttcgggc tgcggcagca gtaggggcga tcggctcgga ggagagctac
      181 cgcgctctac ttcagtcgat cgtcgaggtg gcccgcggca ttttcggcgc caaggcctcg
      241 tcggtgttcc tgctggacga ggagaccgac gagctcgtat tcgaggccgt cagcggggag
      301 ggcgagggag acctgctcgg cgtgcggttt ccggcgggta cgggcatcgc cggctgggtg
      361 ctcatgacgc ggcagccgct cgtgctcgag gacgtcgcga ccgacccgcg cttcgcgcgc
      421 gaggcggccg agcgcaccgg ctacgtgccg aagagcatga tggcggtgcc gctcttgaac
      481 gaggagcggg ccctcggcgt cctgaacgtg ctcgaccggc cgcaggaggc cgccttcacc
      541 ctttccgaga tggatctgct ctcgctgttc ggcaaccagg ccgcgatcgc cctcgatctg
      601 ctgctgcggg cgcggcgggc gcgcgccgcg ctcgcgggcg aaggctcggc tgccgaggcg
      661 gcgatcgcac gcgtcgccgc tgctctcgag gacgacgaag gccggctcga cccgacgggc
      721 gtcgcgttgc tcgaggcact cgccgacgtg cttcagaagc gcgccggggc ggtctgaggc
      781 cgccccgacg ttcgtctcga tctgcgcgca gagagccggt tactcgccga gatccacgag
      841 gcctgacccg tcgtcgagag cacccttctc gccctcgccg acgtccaatg cacccttctc
      901 cccttcgccc acgtcgagca tgtgaccctc gacgtccggc tcttcgccga cgtcgacagc
      961 ttcgcgctcc tcaccgatgt ctacagcttc gcgctcgtca ctcacgtctg cctcctttgc
     1021 agggtggacc gtgattcgaa ctagcgaata gacgtgcggc ccgcccgaaa tcttcccttg
     1081 tgcgcttcga catggcaacc tcggctgaga tgctgcgccc gtgggacgga tcgcagaccg
     1141 cctgcgcgag gggtttgcgg ccgctcacgc cgtagccgcg aaccgcgacc tgcgcggcct
     1201 tcaactggcc ggggtcggct ccgtcctcgg caactgggct tacctcgtcg cgctgctcgt
     1261 ttacgcgtac gacgcgggag gaccggcggc ggtcgggctg gtcagcgtgc tccgcatgat
     1321 ccccgcggct gtgtcggcgc cgttcgtctc cgtgctcgcc gaccgtctga gccgtcgcgg
     1381 cgtgatgatt gcggccgaca tcctccgcgc ggggctgatg ctgggtgcgg gcgcggtcat
     1441 ctgggccgac ggcccgccgt gggtcgtgta cctgatcgtc acgttctcga gcatcgtcgc
     1501 gacgagcttc cgacccgcgc agtcggcgct gctgccggtt ctggcccgtg gcccggagga
     1561 gctcacggcc gcgaacgtcg cggccgcggc gatcacgagc atcggcgccg tcatcggccc
     1621 ggcaatcggc gcggcggtgc tcgccgtctc cagcacggag gtggtcttcg caatcaactc
     1681 gctgacgttc ctctggtcgg ccgccctgat cctcggcgtg cgggagccgg acggaaccgg
     1741 gcccgcccgg ggcggcaacc cgttcggccg cgagctggtg gcgggcctgg ccgtgctcgc
     1801 aggggaccgc gggatgaggt tcctcaccat cctctacgtc gcgcaaacgg tcgtggcggg
     1861 aacgctcaac gtgttgatcg tcgtgaccgc gttctccctg ctcgacaccg gcgagtccgg
     1921 ggtcgggctc atcaacgcgg cgatcggggt gggcgggatc gttggcgggg ccgtcgccct
     1981 cgcgctcgtc gggcgacagc gtctcgcggg ggatttcggc gtcggcctcc tgctcttcgg
     2041 tgctccgctc gccctgattc cggtgtttcc ggagctggcc cccgtgcttg ccctgttcgc
     2101 cgtagtgggg atcggcaaca cgctcgtcga cgtcgcggcg agcacgctgc tgcagcgaac
     2161 cgtcccgaac gacgtcctcg ctcgtgcatt cggcgccctc caggcgctcc tgctcggtgc
     2221 gatcggcgtg ggtgccctca tcgcacctct cctcgtcgag acggtcggca acacggcaag
     2281 cctcgtggct gtgggcgcgc tgctccccgt cgtcggcgtc gctgccctgc cgggactccg
     2341 cgggatcgac cgtcgcgggc ga
//
LOCUS       DBNGNK010000208         4271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1972859,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000208 DBNGNK010000000
VERSION     DBNGNK010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1972859"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="AC150V_06160"
     CDS             <1..544
                     /locus_tag="AC150V_06160"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3762543.1"
                     /translation="QGSVGEARWTYAVSKLAGEHMAHAYHDEFELPAVCVRPFNVYGP
                     GQIGGGAIRAFIEAALAGGDLIVHGDGSQIRAWCYVDDMVDGLMLALEHPKAVGESFN
                     IGNARSAVTIYDLAQRVKRLTGCPGEIVFRPLHYTDVELRIPNVDKARELLGFESQVE
                     LDEGLSRTIAWYRQKLGVPA"
     gene            541..1635
                     /locus_tag="AC150V_06165"
     CDS             541..1635
                     /locus_tag="AC150V_06165"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR3762544.1"
                     /translation="MSRTEKLREIRLARPDVGAAELAAVAEVLESGQLTMGPKVAEFE
                     RALAAACGVADAVAVSSGTAALHLAMLALGVGPGDEVIVPAYTFPSTANAVELCGARA
                     VLADVRPETFNVDPEHVSGAVTPRTRAVLVVHLFGRPAEWEAIETAVPSEVALVEDAA
                     GALGARRRGVACGGLGAMGCLSFHPRKIVTTGEGGAITTDVAAFADDARRLRHHGIAP
                     RGDFDISTPGLNYRLADVLCAVGLPQVARLEKLLRAREHVASLYTERLHDLVETPAAD
                     QGDRHGWQAYVVQLDRRDEALAALRGEGIEAQIGTHALHRLSAYRDRGDFPGAERSYE
                     RALALPFAASLSVADVDRVCEVLAAFVGAS"
     gene            1831..2406
                     /locus_tag="AC150V_06170"
     CDS             1831..2406
                     /locus_tag="AC150V_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3762545.1"
                     /translation="MATTTKRQTAEERREAVLDAARNEFALHGLHGASTDVIARRAGI
                     SQPYLFRLFGSKRDLYLATIEKCFDRTLETFRAAASGASGPEALHAMGKAYDGLIDED
                     RTMLQGQLQAYAASVEDDDVREITSRGFGRLVDYVEAVSGGDRSTVAAFFAKGMLINV
                     LASLGVSLAGEAGEPWARRIVEGCREPEPSH"
     gene            2476..3885
                     /locus_tag="AC150V_06175"
     CDS             2476..3885
                     /locus_tag="AC150V_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR3762546.1"
                     /translation="MPARASTLWTFAITSIALFMVSLDNLVVTTAIPSIRADLGATLE
                     ELEWTVNAYTLTFAVLLLTGAALGDRFGRRRIFAVGVGIFTLGSVLAALAPTADALIL
                     GRAVQGFGGAIVMPLTLTLLSAAVSPERRGLAIGLWGGIGGLAIAIGPLVGGGIVEGI
                     AWQWIFWVNVPIGIALIPLALARLRESYGPNSRLDVPGLALASSGLFGIVWGLIRGNG
                     EGWTSPQILLSLGVGVLLVAAFVAWELRTPQPMLPIRFFRNRAFSLANAAGFLMFFGM
                     FGSIFLLIQQLQSAQGYGPLAAGAAILPWTLAPMFISPWVGALVDRIGEKPFLVAGLT
                     LQASGLAWLAAIASPTTSYLSLVTPFVLSGVGMSLFFAPSASLILRSVKPDEEGQASG
                     AMQAIRELGGVFGVAVLASVFARSGGFETPQTITDGVGPAVFVGAALVAVAALIAAAL
                     PGRRRSAKARTQEPALDAV"
     gene            3959..>4271
                     /locus_tag="AC150V_06180"
     CDS             3959..>4271
                     /locus_tag="AC150V_06180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762547.1"
                     /translation="MLTTGNTAKLYCLERIAARAAERGPLTILDLGCGRGANFVGLLT
                     RHPDVTYVGIDTDPEACAAARVTLSGFRAEIVRGSALGLDRGQFDVVASLSVLEHIRD
                     RR"
BASE COUNT          571 a         1497 c         1475 g          728 t
ORIGIN      
        1 gcaggggtcg gtcggcgagg cacgctggac gtacgccgtc tccaagctcg ccggcgagca
       61 catggcgcac gcctaccacg acgagttcga gctgccggcg gtctgcgtcc gccccttcaa
      121 cgtctacggg cccggccaga tcggcggtgg cgcgatccgg gcgttcatcg aggcggcgct
      181 cgcgggcggc gatctgatcg tccacggcga cggatcgcag atccgcgcgt ggtgctacgt
      241 ggacgacatg gtcgacgggc tgatgctcgc tctggagcac ccgaaggccg tcggcgagag
      301 cttcaacatc ggcaatgcgc gctcggccgt gacgatctac gaccttgcgc agcgggtcaa
      361 gcggctgacc ggctgtccgg gagagatcgt cttccgaccg ctgcactaca ccgatgtgga
      421 gcttcggatc ccgaacgtcg acaaggcgcg cgagctcctc ggcttcgagt cccaggtcga
      481 gctcgacgag ggcctgtcgc ggacgatcgc ctggtaccgg cagaagctcg gcgttcccgc
      541 gtgagccgaa cagagaagct ccgcgagatt cgcctcgcgc gaccggacgt cggcgcggcc
      601 gagctggccg cggtcgccga agtgctcgag tcgggccagc tgacgatggg ccccaaggtg
      661 gccgagttcg agcgcgccct tgcggcggcg tgcggggtgg cggacgctgt cgcggtttcc
      721 tcggggacgg cggcgctcca cctcgcgatg ctcgcgctcg gcgtggggcc cggcgacgaa
      781 gtcatcgtcc ccgcctacac cttcccctcg acggcaaacg ccgtcgagct gtgtggggcg
      841 cgagcagtcc ttgcggacgt tcggcccgag acgttcaacg tcgacccgga gcacgtgtcc
      901 ggcgctgtga ctccacgaac tcgtgccgtg ctcgtcgtgc atctcttcgg gcgccccgcc
      961 gagtgggagg cgatcgagac tgcggttccg tcggaggtcg ccctggtcga ggacgccgcc
     1021 ggcgccctcg gagctcggcg tcggggcgtc gcctgcggcg ggctcggcgc gatggggtgt
     1081 ctctcgttcc acccccggaa gatcgtgacc accggggagg gaggtgcgat cacgacggac
     1141 gtggccgctt tcgccgacga tgcgcggcgg ctccgtcacc acggaatcgc gcctcgcggc
     1201 gacttcgaca tctccacacc cggcctcaac taccggctcg ccgacgtcct ctgcgccgtg
     1261 gggctgccgc aggtcgcgcg gttggagaag ctcctccgtg cacgcgagca cgtcgcgtcc
     1321 ctctacaccg agcggttgca cgacctcgtc gagacgccgg cggcggatca gggcgaccgg
     1381 cacggctggc aggcctacgt cgtccaactc gatcggcggg acgaagcgct ggcggcgctc
     1441 cgcggcgagg ggatcgaggc gcagatcggg acgcacgcgc tccatcgtct gtcggcgtac
     1501 cgtgaccgcg gcgacttccc gggcgccgag cggtcgtacg agagggccct cgcccttccg
     1561 ttcgcggcgt cgctcagcgt cgctgacgtc gaccgcgtat gcgaggtcct ggcagccttc
     1621 gtcggcgctt cgtaaccggc gacccattcc gccgcggccg ccggtcctcg cggccgatgc
     1681 cggcacgaaa gagcgtggcg agggaacggg gactgtcccc ggacagggct cgttcggccc
     1741 tggcgaacgt gtgccgaaag cgcgtctgtt cttccacagg aggcgccggg acttgacaag
     1801 tcgctattgt gagtgaatac tcactactac atggcgacca cgacgaagcg gcagacagcg
     1861 gaggagcggc gtgaggcggt cctcgatgcc gcacgcaacg agttcgctct gcacgggctc
     1921 cacggcgcgt ccacggatgt catcgcccgc cgcgcgggga tctcgcagcc gtacctattt
     1981 cgcctcttcg gctccaagcg cgacctctat ctcgcgacga tcgagaagtg cttcgatcga
     2041 acgctcgaga cgtttcgcgc agccgcctcc ggcgcgtccg gccccgaggc gttacacgcc
     2101 atgggaaagg cgtatgacgg cctgatcgac gaggatcgaa cgatgctcca gggtcagctc
     2161 caggcctacg cggcctccgt cgaggacgac gacgtccgcg agatcacttc gcgcggcttc
     2221 ggccggctcg tcgactacgt ggaggcagtc tcggggggcg accggagcac cgtggcggcg
     2281 ttcttcgcaa agggaatgct gatcaacgtg ctggcctcgc tcggcgtctc gctcgcgggg
     2341 gaggcgggcg agccgtgggc gcgccggatc gtggagggtt gcagggagcc cgagccctcc
     2401 cattgattcc gtttctctct tttttctcca agacatgagt aatcactcac tacttacgaa
     2461 tcgaaaggag cagcgatgcc cgctcgagcc tcaaccctct ggaccttcgc gatcacctcg
     2521 atcgcgctct tcatggtctc cctcgacaac ctcgtcgtga cgaccgcgat tccgtcgatc
     2581 cgggcggatc tcggtgccac gctcgaagag ctcgagtgga ccgtgaacgc ctacaccctg
     2641 accttcgccg tgctgctcct caccggtgcc gcgctcggcg accgcttcgg ccgtcgcagg
     2701 atcttcgccg tcggtgtggg catcttcacg ctcggctcgg tgctggcggc gctggctccc
     2761 acggcggacg cgctgatact cggtcgcgcg gtgcagggat tcggcggcgc gatcgtgatg
     2821 ccgctcactc tcacgctcct cagtgccgcc gtctcgccgg agaggcgcgg actcgcgatc
     2881 gggctctggg gtggcatcgg cggtctcgcg atcgcgatcg gcccgctcgt gggcggcggg
     2941 atcgtcgagg ggatcgcctg gcagtggatc ttctgggtga acgtcccgat cggaatcgca
     3001 ctcatccctc tcgcgctggc gcgcctccgc gagtcgtacg ggcccaactc tcgcctcgac
     3061 gtccccggcc tggcgctcgc aagctctggc ctcttcggga tcgtatgggg tctgatccgc
     3121 ggcaacggcg agggctggac tagcccgcag atcctgctgt cgctcggggt cggggtgctc
     3181 ctcgtcgccg cgttcgtggc ctgggagctc cgcacgcccc agccgatgct gccgatccgc
     3241 ttcttccgca accgggcgtt ctcgctcgcg aatgcggcgg gcttcctgat gttcttcggg
     3301 atgttcggat cgatcttcct cctcatccag cagctccagt cggcacaggg ctacggcccg
     3361 ctggccgccg gagcggcgat cctgccgtgg acgctcgccc cgatgttcat ctccccgtgg
     3421 gtcggcgccc tcgttgaccg catcggcgag aagcccttcc tcgtggccgg cctcacgctg
     3481 caggcgagcg gcctggcgtg gctcgccgcg atcgcgtcgc cgacgacgtc ctacctctcg
     3541 ctcgtgacgc cgttcgtcct ctccggtgtc ggcatgtcgc tcttcttcgc accgtcggcg
     3601 agcctcatcc tccgttcggt caagcccgac gaggagggtc aggcctcggg cgcaatgcag
     3661 gcaatccggg agctcggcgg tgtcttcggc gtcgccgtcc tcgcttccgt gttcgctcgc
     3721 agcggcggct tcgagacgcc acagacgatc accgacggcg tcggtcccgc cgtcttcgtc
     3781 ggagccgcgc tcgtcgccgt tgccgcactg atcgcggccg cgctccccgg ccgtcggcgc
     3841 tcggcgaagg ctcgaacgca ggagccggcg ctcgacgccg tctagcgaat ccgatcgaac
     3901 gggccccggc tgcgcggcgg ccggggcccg ttcggccgag tagtctggcg gtgccgcctt
     3961 gctcacgacc ggcaacaccg cgaagctcta ctgcctcgag cgaatcgccg cgcgggccgc
     4021 agagcgcggc ccgctcacga tcctggatct cgggtgcggc cggggcgcga acttcgtggg
     4081 cctgctcacg aggcaccccg acgtgaccta cgtcgggatc gacacggatc cggaagcctg
     4141 cgcggcggca cgcgtgacgc tctccgggtt tcgtgccgag atcgtgcgcg gcagcgcctt
     4201 gggcctcgat cgcgggcagt tcgacgtcgt ggcgtccctc agcgtgctgg agcacatccg
     4261 tgaccgacgt t
//
LOCUS       DBNGNK010000209         4586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1758454,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000209 DBNGNK010000000
VERSION     DBNGNK010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1758454"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..690)
                     /locus_tag="AC150V_06185"
     CDS             complement(13..690)
                     /locus_tag="AC150V_06185"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR3762548.1"
                     /translation="MAGHSVDEYLETIYFLAFPIGEYRPATGSTAIASRVAEMLGVSR
                     ASAGEMLKRLEADGLVERGEQREAILTQAGIERAERVVRKHRIIERFLTDFMGYTAAE
                     SHDQADELGDTFTDDMIERINDRLGHPDRCPHGWPVETHVEQRENNELVSLAELEPGA
                     RAEIVRLAEHDGELLHWFYDEGFAPGARVEIQEAQPAAGQFKVELDGDERAISEKAAL
                     GLYVRSA"
     gene            complement(701..2284)
                     /locus_tag="AC150V_06190"
     CDS             complement(701..2284)
                     /locus_tag="AC150V_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3762549.1"
                     /translation="MNRGAGRRPPLALVVAAGIVVAALLLPAAYLIVRIVDGGADALD
                     VLVDRRTWELVWSTTLLVVGVVVATIAVGVPLAWLVVRTDLPGRRAWAVASALPLVIP
                     SYVAALSLLGAFGERGLLQRILGVERIPDITGYWGALLALTLATYPYVFLLTQAALRN
                     LDPSLEEAARGLGVSRTRSFLRNTLPTLRQAVGAGALLVALYTLSDFGVVSLMRYDAL
                     TRAIFVQYRSLFDRTPAAVLGLVLVALTLVVLVVEWRTRTRGRLYRTSPGVSRRAAPV
                     RLGRWRWPALAFCSLVVGFFLAVPAAVLGYWLVRGIRNERDLSFPLEEAFNSLGASSL
                     AALIAVLAALPIAVLALRYPSKRSAALERLTYTGNALPGIVIALALVFFAVRYASPVY
                     QTVALLVFAYVVRFFPQGLSGVESALQQVNPRLEEAARGLGRGPIGTATRVTAPLIRS
                     GLAAGAALVFLSAMKELPATLLLRPIGFETLATEVWKFTSVGAYSQAALPALVLIAVS
                     APLLYLVSAERRSGGGAPG"
     gene            complement(2281..3285)
                     /locus_tag="AC150V_06195"
     CDS             complement(2281..3285)
                     /locus_tag="AC150V_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017584201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HIR3762550.1"
                     /translation="MTRFCTGVLALTALLLLAGCGGDSKDTLTVYSGREEELVAPLLE
                     QFQESSGIDLEVRYGDSAELAATIAEEGDNSPADVFFAQDPGSLGAVSDQLAQLPDEI
                     LDRVDEQYRADDGSWVGTSGRVRVIVYNTDVLAEDEVPDSVFDLTDPEWKDRVGIAPT
                     NASFQAFVSAMRLSEGEEATRQWLLDLKENGAKTYEKNTPIVEATAAGEIDLGLVNHY
                     YLYLVREEQPDAPIENHFLEAGDAGALVSVAGVGVLASSDNREGAEQFVEFLLSDEGQ
                     GYYVNEAEEAEYPLIGGIEPREGLPPLSAQAGPSIDLGALGGELTSTVELLRETGWLS
                     "
     gene            complement(3351..4361)
                     /locus_tag="AC150V_06200"
     CDS             complement(3351..4361)
                     /locus_tag="AC150V_06200"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762551.1"
                     /translation="MIQLDAVSKAFGDTVAVGGASLCVEQGEVVALLGPSGCGKTTLL
                     RLVAGFEEPDAGTVHVAGAPVAGPGCWVPPEKRRVGIVFQDYALFPHLTVAENVGFGL
                     PRRERARQVAWLLALVGLCGLGERYPNQLSGGQQQRVALARALAPKPELVLLDEPWSN
                     IDPHLRCELRDELQSVLRPLGVTVVLVTHDREEAFSLADRIALMRDGRVVQEGAPEEL
                     YLNPSDRWAAEFVGAGNFVSGEVDGAVVRTALGAFTANGASGERRAVEVLVRPELLEL
                     EPDPDGAAEVVGREFRGHDVFYRVLLDDVELVSHRPSTEVVPLGARVRIRLHEGRIAV
                     FG"
BASE COUNT          789 a         1580 c         1589 g          628 t
ORIGIN      
        1 gacttcgggc gatcaggcag agcgaacgta gaggccgagc gcggccttct cgctaatggc
       61 gcgctcgtct ccgtccagct ccaccttgaa ctggcccgcc gcgggctgcg cctcctggat
      121 ctcgaccctc gcgcccggag cgaagccctc gtcgtagaac cagtggagca gctcgccgtc
      181 gtgctcggcg agccgcacga tctcggcgcg cgcgcccggc tcgagctccg ccagcgagac
      241 gagctcattg ttctcgcgtt gctcgacgtg ggtctcgacg ggccagccgt gcggacaacg
      301 atccggatga ccgagccggt cgttgatccg ctcgatcatg tcgtcggtga acgtgtcgcc
      361 gagctcatcc gcctggtcgt gggactcggc cgccgtgtag cccatgaagt cggtaagaaa
      421 gcgctcgatg atgcggtgct tgcgcaccac acgctcggcg cgctcgatgc ccgcctgggt
      481 gaggatcgcc tcgcgctgct cgccgcgttc gacgagccca tcggcctcga gccgcttgag
      541 catctcgcct gccgaggcac gcgagacgcc gagcatctcg gcgacccggg acgcgatggc
      601 ggtcgagccc gtcgccggtc ggtattcccc gatcggaaaa gccaggaagt agatcgtctc
      661 gaggtactcg tcgacgctgt gtcctgccac gtcgtcgagt ctacccggga gccccacccc
      721 cggatcggcg ctctgccgag accaagtaga ggagcggcgc ggagaccgcg atcaacacga
      781 gagccgggag cgccgcctgc gagtacgcgc cgacggaggt gaacttccac acctcggtgg
      841 cgagggtctc gaagccgatc ggccggagca ggagggtggc cggcagctcc ttcatggcgc
      901 tgaggaagac gagagccgcg cccgcagcca ggcccgagcg aatgagcggc gcggtgacgc
      961 gcgtcgccgt cccgatcggg ccccggccga gacctcgagc cgcttcctcc agccgcgggt
     1021 tcacctgctg gagcgcggac tcgacacccg acagcccctg cgggaagaag cggaccacgt
     1081 acgcgaagac gagcagtgcg accgtctggt agacgggtga ggcgtagcgc accgcgaaga
     1141 agacgagcgc gagcgcgatc acgattccgg gcagcgcgtt acccgtgtac gtgagacgct
     1201 cgagggccgc gctgcgtttc gaggggtacc tgagcgcgag cacggcgatc ggcagtgcgg
     1261 ccagaaccgc gatcagcgcc gcgaggctcg acgccccgag ggaattgaat gcctcctcga
     1321 gagggaagga gaggtcacgc tcgttccgga tgccgcggac gagccaatag ccgagcaccg
     1381 cggccggcac tgcaaggaag aagcccacca cgaggctgca aaaggcgaga gccggccagc
     1441 gccagcgtcc gaggcggaca ggtgcggctc ggcgggagac gccggggctg gttcgataga
     1501 ggcggccgcg agtgcgggta cgccattcga cgacgagaac gaccagcgtc agcgcaacga
     1561 gcacgagacc gagcacggcc gccggagtcc ggtcgaacag cgatcggtac tggacgaaga
     1621 tcgcccgtgt cagggcgtcg tagcgcatca gcgacacgac tccgaagtcg gagagcgtgt
     1681 agagggcgac caggagtgcg ccggcgccga ccgcctgtcg cagggtaggc agggtgttgc
     1741 gaaggaacga gcgcgttcgc gagaccccga ggccgcgcgc tgcctcttcg agcgacgggt
     1801 cgaggttccg cagggcagcc tgtgtgagca ggaagacgta agggtaggtc gcgagcgtga
     1861 gcgccagcag agcgccccag taccccgtga tgtcaggaat ccgctcgacg cccaggattc
     1921 gctggagcag cccacgctcg ccgaacgcgc cgagcagcga caacgcggcg acgtagctcg
     1981 ggatcacgag tggcagggcg gaggccacgg cccaggcgcg ccgaccgggc aggtctgtgc
     2041 ggacgacgag ccaggcaagc ggcacgccga ccgcgatcgt ggccacgacg acgccgacga
     2101 cgagcagcgt ggtgctccag accaactccc aggtgcggcg gtcgacgagg acgtcgagag
     2161 cgtcggcgcc gccgtcgacg atccgcacga tcaagtacgc ggcaggcagg agcagcgccg
     2221 cgacgacgat gcccgctgca acgacgagcg cgagaggggg gcggcgtccc gcccccctgt
     2281 tcacgagagc cagccggtct cgcgcaacag ctcgaccgtc gacgtgagct cgccgccgag
     2341 cgcgcccaga tcgatgctcg gcccggcctg ggcagagagc ggaggaagcc cctcacgcgg
     2401 ctcgattccg ccgatcaacg ggtactccgc ctcctcggcc tcgttcacgt agtacccctg
     2461 gccctcgtcc gagagcagga actcgacgaa ctgctccgct ccctcccgat tgtcggaaga
     2521 ggcgagcacg cccacgccgg ccaccgagac gagcgcgccg gcgtcaccgg cctcgaggaa
     2581 atggttctcg atcggggcat ccggctgctc ttcgcgcacc aggtagaggt agtagtggtt
     2641 gacgagcccg aggtcgatct cgccggcggc tgtcgcctcc acgatcggcg tgttcttctc
     2701 gtacgtcttc gcgccgttct ccttcaggtc gagcagccac tgccgcgtgg cctcctcgcc
     2761 ttcggagaga cgcatcgcgc tcacgaaggc ctggaacgac gcattcgtcg gggcaatccc
     2821 cacccggtct ttccattccg ggtcggtcag atcgaacacg gagtcgggca cctcgtcctc
     2881 ggccaggacg tcggtgttgt agacgatgac gcggacacga ccggacgtcc cgacccatga
     2941 gccgtcgtcg gcccggtact gctcgtcgac acgatccagg atctcgtccg gcagctgcgc
     3001 cagttggtcg gagaccgctc ccagcgagcc ggggtcctgc gcgaagaaca cgtcggcggg
     3061 cgagttgtca ccttcctccg caatcgtcgc ggcgagctcg gcgctgtcgc cgtaccggac
     3121 ttctaggtcg atcccgctcg actcctggaa ctgttcgagc agcggcgcca cgagctcctc
     3181 ctcacgcccc gagtacaccg tgagcgtgtc tttcgagtcg ccgccgcagc cggccagcag
     3241 caggagcgcc gtcagcgcga gcacgccggt gcagaacctg gtcatcttcc ctccctgtaa
     3301 ggtctaccct attctctatg taagggtcac cttacatatc cggcgaggtg ctagccgaag
     3361 acggcgatgc ggccctcgtg gagccggatc cggacgcggg cgcccagcgg cacgacctcg
     3421 gtggaggggc ggtgggagac gagttcgacg tcgtcgagga gcacgcggta gaagacgtcg
     3481 tggccgcgga actcgcgacc gacgacctcc gccgcgccgt ccggatcggg ctcgagctcc
     3541 agcaactccg ggcggacgag tacttcgacc gcccgacgct cacccgacgc tccgttggca
     3601 gtgaacgctc ccagcgccgt tcgtacgaca gcgccgtcga cctcgccgga gacgaagttg
     3661 ccggcaccca cgaactccgc cgcccagcga tcggacgggt tcagatagag ctcctccggt
     3721 gccccttcct gcaccacccg gccgtcgcgc atgagggcga tccgatcggc gagcgagaac
     3781 gcctcctccc gatcgtgcgt aacgagaacc accgtcacgc cgagcgggcg gagaaccgac
     3841 tgcagctcgt cgcggagctc gcagcggagg tgggggtcga tgttcgacca cggctcgtcg
     3901 aggagcacga gctccggctt cggcgcgagc gcccgtgcca gtgccacgcg ctgctgctgt
     3961 ccgcccgaga gctggttcgg atacctctcg cccagaccgc agagcccaac gagggcgagc
     4021 agccaggcga cttggcgcgc gcgttcgcgc cggggcaggc cgaagccgac gttctccgcg
     4081 accgtgaggt gggggaagag cgcgtagtcc tggaagacga tcccgacacg tctcttctcg
     4141 ggcggcaccc agcagcccgg gccggcgacc ggagctccgg cgacgtggac cgtgcccgca
     4201 tcgggctcct cgaagccggc cacgagacgg aggagtgtgg tcttcccgca accggaagga
     4261 cccagcaacg cgaccacctc gccctgctcg acgcagagcg aagccccgcc gactgcgaca
     4321 gtgtcgccga acgccttgga gactgcgtcg agctggatca tcgagcagga catgtaaggc
     4381 gaaccttaca tcgcgtgcag gggcaattgc tggatccctc gagcgaggat cgcgtcgcgg
     4441 cgcccaccgg cggggaccgg ggactgtccc ccgacatggc ccgcgcagcc gtggcggaac
     4501 tgtgtcgaaa gtgagtcaag gtccccacag gagctgaggc ccccgcggcg gccgcacccc
     4561 cggctacgct cgccgtcgtg acgctt
//
LOCUS       DBNGNK010000210         3894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1758474,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000210 DBNGNK010000000
VERSION     DBNGNK010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1758474"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..649)
                     /locus_tag="AC150V_06205"
     CDS             complement(161..649)
                     /locus_tag="AC150V_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HIR3762552.1"
                     /translation="MSNVEETKLPGVGVRYTFTTSDGERLGVIAHRGGKRELLVYDRQ
                     DPDACSAVLGLEGDDARTLVDLLGGSGVSERLEETIRQSIEGLVFEWVEVDSQSPYEG
                     SAIADIGLRSKTGASIVAVVRGDETITSPRGDVRLATGDTVVLVGSPAAIGEAVAVLR
                     GA"
     gene            complement(697..1152)
                     /locus_tag="AC150V_06210"
                     /pseudo
     CDS             complement(697..1152)
                     /locus_tag="AC150V_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(1204..1626)
                     /locus_tag="AC150V_06215"
     CDS             complement(1204..1626)
                     /locus_tag="AC150V_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016701.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR3762553.1"
                     /translation="MTSAPLTGRCMCGGVRFEITEPLGAAGYCHCTRCQRRTGTSSSA
                     QARLAPGSLTIVAGEDLIRSFDPPDGFAKLFCAACGSALFGRHPQDPNVLSVRLGTLE
                     GDPGVRPSFHQYTAYASSWEPLPDDGLPRFEEGRPPDA"
     gene            1790..3823
                     /locus_tag="AC150V_06220"
     CDS             1790..3823
                     /locus_tag="AC150V_06220"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3762554.1"
                     /translation="MGIRVGIDTGGTFTDLVAVDEESGRWHLAKVPSNPENPVAAIAA
                     ALETAHFDAAEVSFVVVGTTIGINAVLTRSGADVVLLTTDGFEDIPYIQRINRKNHYD
                     FTWRKPTPLVARPDCIGVDERLDEEGVVVDEIDLDGLGARLEERLAERGGNGVAVAVC
                     YLFSYLNPTHEQATRDYLHEQFPDLPVSLSHEVAPIWREYERGVTVTVDAALKPLVER
                     YVDGVSEALTVSGIEHGWALLKSNGGGALADEARERPAHLLLSGIAGGAIGGAHMARA
                     SGAEEAILLDMGGTSCDVCLVLGGEPLYSSDFEIEFGLPVSVPSVSTRTIGAGGGSIG
                     WVDPGGFLQVGPQSAGASPGPACYAQGGSEATLTDANVLLGRLDASFFLGGQMRLEAD
                     LSRESLGRLGAKLDLDAVDVASAMVRVANENMANAIRIVTVEEGIDPRGLALVAFGGA
                     GPTHACEIADAIGMRRVVVPPSPGLCSAFGALAAAPRVDAVRSVYLTDARATPGQLDG
                     LFTGLEEQATRDIAAQGLQGSPSVRRSIAMRYQGQNYEQEVAVPEGTIDDGAYAHFFQ
                     EYGRLYEEFYGYRLDGIPIELVRLSVVASGDPPVFSGLDLGEDVATPADERDVHFPEH
                     GFVRAAVLRRASIEAGFSVAGPVIVESMDSTVVVPPEWSLRTASDGILDLTRA"
BASE COUNT          586 a         1341 c         1386 g          581 t
ORIGIN      
        1 gatcgcgccg atcgccagtc ccccaatgag gtagacgggg atcggggaga tgccgaagac
       61 cgccgcgagc cgtgcgagca cggcgagcac gaggaggacg agcccgatct cgatgaggag
      121 tcgcgccgtg tcgccgctgc tcgcggcgac gaaagcggcg tcaggcaccg cggagaacgg
      181 cgacggcctc gccgatcgcc gcgggcgagc cgacgagcac gacggtgtct ccggtcgcga
      241 gccggacgtc tccgcgcggc gacgtgatcg tctcgtcacc gcgcacgacg gcgacgatcg
      301 acgcgcccgt cttggagcgg aggccgatgt ctgcgatcgc gctcccttcg tagggagact
      361 gcgagtcgac ctcgacccac tcgaagacga gtccctcgat cgactgccgg atcgtctcct
      421 ccaaccgctc gctcaccccc gacccgccaa gaaggtcgac gagtgtacgc gcgtcgtcgc
      481 cctccaggcc gagcacagcg ctgcaggcgt ccgggtcttg ccggtcgtac acgagcagct
      541 cgcgcttgcc gcctcgatgc gcgatcacgc ccaggcgctc accgtccgac gtggtgaacg
      601 tgtagcggac accgacaccg gggagcttgg tttcctcgac gttgctcacg ctggtcattc
      661 tcgcaaaggg ccgccgagtg gcgccgcagc catagctcag tcgacgatca gcacgtcggc
      721 cggcgcgcct cgctcgacct ctgcggccgt gtccgtgccg agcaccttgc tgaagaatcc
      781 gccctggtgc gcgccgacga cgatcaggtc ggctcggcgg cgctccgctt catcgacgat
      841 cgcggcggcg gggttacctg caccccaggt gtagtccgcc tggtagccgg cgaggcggat
      901 ctgctctcga gcatgctcga gtcacggctc gagggactcc ggcagtgtgc ccggcgccct
      961 cgcgggcggc ccgtcgtcga gcgtgccgaa gttgcggggg tcgtccgggt cgagcggcat
     1021 ctcggcgacc atgacgacga cgagcttccc cttgcgatcg cgtgcctcct cgatcgcgcg
     1081 gccgagcgcc cgcttcgcgg gttccgtgtc gtcgtagccg accacgacgt tcctgctcat
     1141 gcccgagagc atcgcatgtc cgcgcacggt cgtgggcaga gactccgagc cgcgggggcg
     1201 cgctcaggcg tccggcgggc ggccctcttc gaagcgcggc aggccgtcgt cgggaagcgg
     1261 ctcccaagac gacgcgtacg ccgtgtactg gtggaagctc ggccggacgc cggggtcgcc
     1321 ctcgagcgtt ccgagacgaa cgctgagcac gttcggatcc tgcgggtggc gtccgaagag
     1381 ggccgagccg caggctgcgc agaaaagctt cgcaaagccg tccggcggat cgaacgagcg
     1441 gatgagatcc tctcccgcga cgatcgtcag cgatcccggt gcgaggcgcg cctgcgccga
     1501 tgacgacgtc ccggtccgtc gctggcaccg cgtgcagtga cagtacccgg ccgctccgag
     1561 cggctccgtg atctcgaacc gaacgccacc gcacatacag cggccggtga gcggcgcaga
     1621 tgtcatgggc gcaggctaga cgctggcggg ctcgacggac gaccgccgtc ggggactgcg
     1681 cgcccgcgcc aagccccgcg aaggagatcc ccgcgcccag catggccgca aaagcggata
     1741 cagcctcggc ccgtcgacac gcaccggcta gagtcggacg tctcgcaccg tgggcattcg
     1801 cgtcggcatc gatacaggcg gcaccttcac ggatctcgtc gcggtggacg aggagtccgg
     1861 gcggtggcac ctggccaagg ttccgtcgaa ccccgagaac cccgtcgccg ccatcgccgc
     1921 cgcgctcgag acggctcact tcgacgcggc cgaggtctcc ttcgtcgtcg tcgggacgac
     1981 gatcggcatc aacgccgtcc tgacccgcag cggcgccgac gtggtgctgc tgacgacgga
     2041 cggcttcgaa gacatcccgt acatccagcg aatcaaccgg aagaaccact acgacttcac
     2101 ctggcgcaag ccgacgcctc tcgtcgcccg cccggactgc atcggtgtgg acgaacgcct
     2161 cgacgaggag ggtgtcgtgg tcgatgagat cgacctcgac ggtctgggcg cccgtctcga
     2221 ggagcggttg gccgagcggg gaggcaacgg tgtggcggtc gcggtctgct acctcttttc
     2281 gtacctcaat ccgacgcacg agcaggccac ccgcgactac ctgcacgagc agtttcccga
     2341 tctgccggta tcgctctcgc acgaggtggc gccgatctgg cgcgagtacg agcgaggtgt
     2401 gacggtgacg gtggacgccg cgctcaagcc tctggtcgag cgatacgtgg acggcgtctc
     2461 cgaggccctg accgtgagcg ggatcgagca cggctgggcg ctgctgaagt cgaatggcgg
     2521 cggcgccctc gcggacgaag cgcgggagcg cccggcgcat ctcctgctgt ccggcatcgc
     2581 gggcggcgcg atcggcggcg cgcacatggc gcgagcgagt ggggcggagg aggcgatcct
     2641 cctggacatg ggcggcacga gctgcgacgt ctgcctcgtg ctcggcggtg agccgctcta
     2701 ctcatccgac ttcgagatcg agttcggcct gccggtgagc gtgccgagtg tctcgacaag
     2761 gacgatcggc gcgggcggcg gctcgatcgg atgggtcgac ccaggcggct ttctgcaggt
     2821 cggcccgcag agcgcgggcg cctcgccggg gccggcgtgc tacgcgcagg gtgggagcga
     2881 ggcgacgctg acggacgcga acgtgctgct cgggaggttg gacgcgtctt tcttcctcgg
     2941 cggacagatg cggctcgaag ccgacctgag ccgcgagtcg ctgggccggc tgggagcgaa
     3001 gctcgacctc gacgcggtgg acgttgcgtc cgcgatggtt cgggtcgcca acgagaacat
     3061 ggccaatgcg atccgcatcg tgacggtgga ggagggaatc gatccgcgcg gcctggcgct
     3121 cgtcgccttc ggcggcgccg gcccgacgca cgcttgcgag atcgccgacg ctatcggcat
     3181 gcgccgcgtg gtcgtgcccc cgagcccggg cctgtgctcc gcgttcggcg ccctcgcggc
     3241 ggcaccgcgc gtcgacgcgg ttcggagcgt ctacctcaca gacgcgcggg cgacgcctgg
     3301 ccagctggac ggactgttca cagggctgga ggagcaggcg accagggaca tcgccgccca
     3361 ggggctgcag ggctcaccga gcgtccgccg gtcgatcgcg atgcgctacc agggtcagaa
     3421 ctacgagcag gaggtcgccg tgccggaagg gacgatcgac gacggcgcct acgcccactt
     3481 cttccaggag tacggtcgcc tctacgagga gttctacggg tatcggctcg acggcatccc
     3541 gatcgagctg gtgcggctct cggtggtcgc ctcgggtgac ccgcccgtct tctccgggct
     3601 cgacctcggc gaggacgtcg cgacgcccgc ggacgagcgc gacgtccact tcccggagca
     3661 cgggttcgta cgcgcggccg tcctgcgccg tgcctcgatc gaggctggat tctccgtcgc
     3721 cgggccggtg atcgtcgagt cgatggactc gaccgtggtc gtcccgccgg agtggtcgct
     3781 gcggacggcg tccgacggca tcctcgacct cacacgcgca tgaccgacac cgtcaccctc
     3841 tcgatcgtca acaacgcctt cgtgaacatc tgccgcgaga tgggcatcac gatg
//
LOCUS       DBNGNK010000211         3771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_899512,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000211 DBNGNK010000000
VERSION     DBNGNK010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_899512"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..524)
                     /locus_tag="AC150V_06225"
     CDS             complement(108..524)
                     /locus_tag="AC150V_06225"
                     /inference="COORDINATES: protein motif:HMM:NF021575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HIR3762555.1"
                     /translation="MTPLRISIAAAIASVLLILVVLELIRGRRLKERYALLWLATGTI
                     LLVLSLWRGGLDTIAGWLGVSGYPPAILFAAATLFVIIVLLHYSTVLSRMDDENTVLA
                     QRLALAEQRLAELEARSAEIGETEPEAASDRAGAAG"
     gene            complement(521..1246)
                     /locus_tag="AC150V_06230"
     CDS             complement(521..1246)
                     /locus_tag="AC150V_06230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3762556.1"
                     /translation="MSARTLAIVPAHNEEAAIGRVVSEIRALSPDFDVVVVDDGSTDA
                     TAAVAATRGAAVVSLPFNLGIGGAVQTGFKYALENGYERAVRLDGDGQHDPAELPKLL
                     AILESGEADLVTGSRFAEGSNGYRPPVARRIGIVFFAKLVSLLTRQKVTDTTSGFQAL
                     NHRGIALFADDYPSDYPEVEATVLASKHRLRLVEVPVAMRAREYGESSIGFFSSIYYM
                     LKVTLALFLAMLRKYTLPEEDPR"
     gene            complement(1243..2463)
                     /locus_tag="AC150V_06235"
     CDS             complement(1243..2463)
                     /locus_tag="AC150V_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CgeB family protein"
                     /protein_id="HIR3762557.1"
                     /translation="MKAPRILAVASAVDLDYRYGCTPAWWQLWKGMYEAGVDLVVTPY
                     RGRPVESPWWRVAPNPAYREGETYATLRDGLARLKGDRYLRRREDSPDDSPLDRLTRE
                     TIWRVVTPRWRRHLERLVEQERPDAVVVFTVPMAHLRGIAPALRDRFGIPIVYYDGDV
                     PMSLPEFGGMDTGFNYYHGADPAEYDLVLSNSEGGLARLRELGARRAEAVYWAADPDF
                     FAPRPVAKEMDVFFYGYGDKFRREWTAAMIGEPSRAASEIDFALGGRDFQGDVGDARA
                     LGDVPFNAFAAAISAARINLNITRRSHATVPGSSTCRPFELASAGAAIVSNPHAGIER
                     WFEPGSEIVVVDDAEQAVAAYRELLGDAAQAEAMGARARERVLDEHTYRHRASQVLDL
                     LGLAVPTATTGVVR"
     gene            complement(2541..>3771)
                     /locus_tag="AC150V_06240"
     CDS             complement(2541..>3771)
                     /locus_tag="AC150V_06240"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR3762558.1"
                     /translation="APITVLVFLQAGLYAPRERRPGWGRIVGSLVLVALVVLAFGLGT
                     DYEFTTTGLIPTAVVTCAVAIGLLRAAYGSISLELMQLAGIRRRAVLVGEGETLRRLH
                     SALAQTHGGIAYEFVGAISLAGEPGLRVLGSSLDDLPEVLELTRPDEVILTEQDFDES
                     RMLAAVETAHRAGVKVRLAPSTTDLLVHEAEYVPGQGAPLFELRPPVFAGVDWVVKRL
                     FDVVVSVVIGLLGVPIWLAVALAVKLDSRGPVFYVDRRIGVGQKEFGMLKFRTMAADA
                     PQRQAELERQNEASGALFKMRDDPRVTRVGRFLRRFSLDELPQLVNVLRGEMSLVGPR
                     PLPLRDYELLDEWHLRRYLVLPGMTGLWQISGRSGLSFDDLVRLDFTYIENWSVWLDI
                     TILVRTVPAVLSRRGAY"
BASE COUNT          620 a         1288 c         1320 g          543 t
ORIGIN      
        1 gaacgcgatg tacgtggacg gatcgccgcc gccgggggtc acctacagaa tcggcgtcgg
       61 gacgagcaac cgcggcgacg agacggactt cggcgacacg ttcgtgatca gcccgccgct
      121 ccggctcggt cgctagcagc ctcgggctcc gtctcgccga tctcggcgga gcgggcctcg
      181 agctccgcca gtcgctgctc ggcaagtgcg agccgctgcg ccagcacggt gttttcgtcg
      241 tccatccgcg acagcacggt cgaatagtgc agcagcacga tgatgacgaa cagcgtcgcc
      301 gcggcgaaga ggatggccgg cggatagccg ctcaccccga gccaacccgc gatcgtgtcg
      361 agcccgcccc gccagagcga gaggacgagc aggatggtgc cggtggcgag ccagagcagc
      421 gcgtaccgct ccttcagccg acgacctcga atcagctcga gcactacgag aatcaggagc
      481 accgacgcga tcgccgccgc gatcgagatg cgcaacggcg tcatcgtggg tcttcctcgg
      541 gcagcgtgta cttccgcagc atcgccagga acagcgcgag ggtcaccttg agcatgtagt
      601 agatcgagct gaagaacccg atcgaggact cgccgtactc gcgggcgcgc atcgccaccg
      661 gcacctctac gagtcggagc cggtgcttgc tcgcgagcac cgtcgcctcc acctccgggt
      721 agtcgctcgg gtagtcgtcg gcgaagagcg cgatcccgcg gtgattgagc gcctggaagc
      781 ccgacgtcgt gtcggtgacc ttctggcggg tcagcaacga cacgagcttg gcaaagaaga
      841 cgatgccaat tctgcgcgcg accggcggac gatagccgtt gcttccctcg gcgaagcgcg
      901 agcccgtgac caggtcggct tcgccgctct ccagtatcgc cagtagcttc ggcagctccg
      961 ccgggtcgtg ctggccgtcg ccgtccagcc ggacggcgcg ctcgtacccg ttttcgagcg
     1021 cgtacttgaa gccggtctga acggcgccgc cgattccgag attgaacggg agggagacga
     1081 cggcggcacc ccgggtggcg gccacggctg cggtcgcgtc ggtcgaaccg tcgtcgacga
     1141 cgacgacgtc gaagtcgggg ctgagcgcgc ggatctcgct gacgacccgg ccgatcgcgg
     1201 cttcctcgtt gtgtgccggg acgatcgcga gcgtgcgcgc gctcatcgga cgacacccgt
     1261 cgtagccgtg ggcaccgcga ggccgagcag gtcgaggacc tgcgacgcgc ggtgccgata
     1321 ggtgtgctcg tcgagcactc gctcgcgcgc tctcgcgccc atcgcttcgg cctgcgccgc
     1381 gtcgccgagc agctcccgat aggcggcgac ggcctgctcc gcgtcgtcga cgaccacgat
     1441 ctcgctcccc ggctcgaacc agcgctcgat tcccgcatgt gggttcgaga cgatcgcggc
     1501 acccgccgag gcgagctcga atggccggca ggtggacgat ccggggaccg tcgcgtgcga
     1561 ccgtcgcgtg atgttcagat tgatgcgcgc tgccgaaatc gccgcggcga acgcgttgaa
     1621 ggggacgtcg ccgagcgccc tggcatcccc gacgtcgccc tggaagtcgc gcccgcccag
     1681 cgcgaagtcg atctccgacg ccgcgcggct cggctcaccg atcatcgccg ccgtccactc
     1741 gcgacggaac ttgtcgccgt agccgtagaa gaagacgtcc atctccttgg ccacgggccg
     1801 gggggcgaag aagtcggggt ccgccgccca gtagacggcc tcggcccggc gtgcgcctag
     1861 ctcgcgcagg cgggccaggc ctccttcgga gttcgagagc acgaggtcgt actcggcggg
     1921 atcggcgccg tggtagtagt tgaatccggt gtccattccg ccgaactcgg gcaggctcat
     1981 cggcacgtcg ccgtcgtagt agacgatcgg gatcccgaac cgatcccgca gcgcgggagc
     2041 gatgccgcgg aggtgcgcca tcggcacggt gaagacaacg accgcgtcgg ggcgttcctg
     2101 ctcgacgaga cgctcgagat gacggcgcca gcgaggtgtg acgacgcgcc agatcgtctc
     2161 gcgggtgagc cggtcgagcg gtgagtcgtc cgggctgtcc tcgcgtcgcc gcaggtagcg
     2221 gtcgcccttc agccgtgcga ggccgtcgcg cagcgttgcg tacgtctcgc cctcgcgata
     2281 cgcgggattg ggcgcgaccc gccaccacgg cgactcgacg gggcgaccgc ggtacggggt
     2341 gacgacgagg tcgacgccgg cctcgtacat tcctttccag agctgccacc aggccggcgt
     2401 gcacccgtag cggtagtcga gatcgactgc cgacgcgacc gcgaggatac gaggcgcttt
     2461 catcggcgag gaagtctagg tatgagcgat gaaggatccg tgatcgcagg ccacgctttc
     2521 ggcactcgcc ggatcgacgc tcagtaggcg ccgcgcctcg aaagcaccgc ggggacggtt
     2581 ctgaccagaa tcgtgatgtc gagccagacc gaccagttct cgatgtaggt gaagtcgagc
     2641 cggacgaggt cgtcgaagga cagcccgctg cgacccgaga tctgccagag accggtcatt
     2701 cccggcagca cgaggtagcg cctgaggtgc cactcgtcga gcagctcgta gtcgcgcaac
     2761 ggcagcggcc tcggccccac gaggctcatc tcgccccgga gcacgttcac gagctgcggc
     2821 agctcgtcca gcgagaaccg gcgcagaaac ctgccgacgc gggtcacgcg cgggtcgtcc
     2881 cgcatcttga acagcgctcc cgacgcctcg ttctgccgct cgagctccgc ctgacgctgc
     2941 ggcgcgtcgg cggccatcgt ccggaacttg agcatgccga actccttctg gcccactccg
     3001 atcctgcggt cgacgtagaa caccgggccg cgggagtcga gcttgacggc gagggcgacg
     3061 gcgagccaga tcggcacccc gagcaagccg atgacgacgc tgacgacgac gtcgaacaat
     3121 cgtttcacga cccagtcgac gccggcgaag acgggcggcc ggagctcgaa gagcggcgcc
     3181 ccctgtccgg ggacgtactc ggcttcgtgc acgagcaggt cggtcgtcga gggtgcgagc
     3241 ctcaccttca ctccggcacg gtgcgccgtc tcgacagcgg ccagcatgcg gctctcgtcg
     3301 aagtcctgct cggtgaggat cacctcgtcg ggccgggtca gctcgagcac ctcgggcagg
     3361 tcgtcgagcg acgagccgag gacgcgcagg ccgggctctc cggccagcga gatcgcgccg
     3421 acgaactcgt acgcgatgcc gccgtgcgtc tgggccaggg cggagtgcag gcgccgcagg
     3481 gtctcgccct ccccgacgag aacggccctg cgccggattc cggcgagctg catcagctcg
     3541 agcgagatgg agccgtaggc ggcgcggagg agcccgatcg ccactgcgca cgtgactacc
     3601 gcggtgggga tcaggcccgt cgtcgtgaac tcgtagtcgg tgccgagccc gaacgctaga
     3661 accaccagcg cgacgaggac cagcgacccc acgatgcgac cccagccggg ccgccgctcg
     3721 cgcggcgcat agagcccggc ctgcaggaag acgagaacgg tgatgggggc g
//
LOCUS       DBNGNK010000212         4519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1470867,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000212 DBNGNK010000000
VERSION     DBNGNK010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1470867"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..126
                     /locus_tag="AC150V_06245"
                     /pseudo
     CDS             1..126
                     /locus_tag="AC150V_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(158..382)
                     /locus_tag="AC150V_06250"
                     /pseudo
     CDS             complement(158..382)
                     /locus_tag="AC150V_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018636269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
     gene            complement(360..1679)
                     /gene="purB"
                     /locus_tag="AC150V_06255"
     CDS             complement(360..1679)
                     /gene="purB"
                     /locus_tag="AC150V_06255"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR3762559.1"
                     /translation="MIERYSRPAMTRIWSEEAKLERWLRVELAALEAWAQLGAIPAEA
                     VEHIRGSAHAPTPQRVAEIERETNHDVAAFVDAVAEGLGEDGRWFHYGLTSSDVLDTA
                     LSLAVQEAGALVLAALDGAVEAVVARAEEHRGAVMIGRTHGVHAEPTTFGLKLAGWAF
                     ALDRDRKRLSRALEGMRVGKLSGAVGTYAATDPELERIACEELGLEPAPVSTQVLQRD
                     RHAEVLAALALLGSTLDRCATEIRHLARTEVREVEEPFGRAQKGSSSMPHKRNPITAE
                     RISGLARVVRANAQVGYENVALWHERDISHSSAERIVLPDSFLAVDYMLDRFTWLVQG
                     LIVRTQRMRENLESSHGLYASQRLLLALIESGLTRDDAYRRVQHHAMQAWEESVDLAS
                     LVRADREIAQRVDLDAVFDESVYTRHVDTIFARLRTVTPAREEPIRA"
     gene            complement(1853..3088)
                     /gene="purD"
                     /locus_tag="AC150V_06260"
     CDS             complement(1853..3088)
                     /gene="purD"
                     /locus_tag="AC150V_06260"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR3762560.1"
                     /translation="MIVLIVGSGGREHALAWKLSSSPSLSELHAAPGNPGIATLGHCH
                     PVGAEDAEALLALARSLAADLVVIGPEAPLVGGVADVLRHAGIAVFGPSSAAARIEGS
                     KSFAKDVMEASFVPTASRLSVARAPCVVKADGLAAGKGVFVCETQAELDAGLRAAAAF
                     GGPVVLEELLVGPELSLFALCDGRELLGLSPAQDFKRAFDGDEGPNTGGMGSYAPVPG
                     VSNADIEVLLDRVHRPVLDELADRGEPFVGCLYAGLIVTAEGPKVLEFNCRFGDPETQ
                     SVLPLVGGDLLEALAAAAAGELAGVSLGRSDGASVAVVLAAGGYPAESDRGSEIAGIE
                     AAEAAGALVFHAGTALHDGRVTTNGGRVLNVVGRGGTIDAARELAYAAIERVSFPGAR
                     YRTDIAERAAGGMTVSLSG"
     gene            3208..3942
                     /locus_tag="AC150V_06265"
     CDS             3208..3942
                     /locus_tag="AC150V_06265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762561.1"
                     /translation="MGRYGEALARSHATAAGVSAGQRALDVGCGPGAFTAVLADILGP
                     ENVAAVDPSEPFAETCRQRVPGADVRVAPAEELPDFGFRFDVVSSQLVVNFMTDARAG
                     IREMSSRARAGGTVASVVWDYAAGMTMLRAFWNAAVALDPEAPDEGRTMAWCTPGELR
                     ELWSTAGLESVETRPLIVEAAYESFADFWDPFPSGIGPTGAYCAALDAEHRESLRREC
                     FRELGSPAGAFTLTARAWFVGGTAAG"
     gene            complement(3955..>4519)
                     /locus_tag="AC150V_06270"
     CDS             complement(3955..>4519)
                     /locus_tag="AC150V_06270"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR3762562.1"
                     /translation="PFVTSSSPIAAGAAVSFGIGPGRIDEVLGVTKAYVTRVGEGPFP
                     SEIQGEAQERVRQLGGEFGTVTGRERRCGWLDLVALRFAVRVNGITALALTKLDVLSS
                     FAELPVCVRYRLPDGSETEEFPAHQSDFHHCAPVFETLSGWEHELSGETLPAAARSYV
                     GYVESALGVPVTLVGTGAGREAVLSLP"
BASE COUNT          677 a         1608 c         1579 g          655 t
ORIGIN      
        1 gccaagccaa ccgagcagac gccgctgacc gcgctgctgc tcgccgacat ctgctacgag
       61 gccggattgc cgccgcagat gctgtcggtc gtgaccggcc tgcccgagga catgggcgac
      121 gaaattactc catcgagcgc ccgtcggaac ggagacgcaa gaggtggttc ggcacgagct
      181 cggccgtgcg cgcgaaccag aaggccgaga ggcctgtgag gacgcggccc ttgtccggga
      241 tcggggttgg gaggacgacg tcgaaggtcg agatccggtc ggatgcgacg aggaggaggg
      301 tctcgggcga gagcgcatag atctctcgca ccttgccgct ggcgacgtgg acggaggcct
      361 caggcacgaa ttggctcctc acgggccggt gtgacggtgc gcagacgggc gaagatcgtg
      421 tcgacgtggc gcgtgtagac ggactcgtcg aagactgcgt cgaggtcgac tcgctgtgcg
      481 atctctcggt cggcgcgcac caagctcgcg aggtcgacgc tctcttccca ggcctgcatg
      541 gcgtgatgct ggacgcgacg gtatgcgtcg tcgcgtgtga gtccggactc gatcagtgcg
      601 agcagcaacc gctggctcgc gtagagcccg tggctcgact cgagattctc ccgcatgcgc
      661 tgcgtgcgga cgatcagtcc ctgcacgagc caggtgaagc ggtcgagcat gtagtcgacg
      721 gccaggaagg aatcggggag gacgatccgc tccgcggagg agtgggagat gtcccgctcg
      781 tgccacagcg cgacgttctc gtagccgacc tgggcgttgg cgcgaacgac gcgcgccagc
      841 ccgctgatgc gctcggcggt gatggggttg cgcttgtggg gcatcgagga cgaccccttc
      901 tgggcgcggc cgaagggctc ttcgacttcg cgcacctcgg tgcgcgcgag atggcggatc
      961 tcggttgcgc atcggtcgag cgtcgacccg agcagcgcga gggcggcaag cacttctgcg
     1021 tgccggtcgc gctggagcac ctgcgtcgag acgggagcgg gctcgaggcc gagttcctcg
     1081 caggcgattc gctcgagctc gggatcggtc gcagcgtagg ttccgaccgc cccggagagc
     1141 ttcccgaccc gcatcccctc gagcgcccgg ctcagccgct tccggtcgcg gtccagcgcg
     1201 aaggcccacc cggcgagctt caggccgaag gtggtcggct cggcgtggac gccgtgggta
     1261 cggccgatca tgaccgcgcc tcgatgctcc tcggcgcgcg ccacgactgc ctcgactgcg
     1321 ccgtccaggg ctgcgagcac gagcgccccc gcctcctgca ccgcaaggga gagcgcggtg
     1381 tcgagcacgt ccgacgacgt caggccgtag tggaaccagc ggccgtcctc accgagaccc
     1441 tctgcaacgg catcgacgaa cgctgcgaca tcgtggttcg tctcccgctc gatctccgcg
     1501 acccgctgcg gcgtcggcgc gtgcgcgcta ccgcggatat gttcgaccgc ctcggccggg
     1561 atcgccccca actgcgccca cgcctcgagg gccgccagct ccacacgcag ccagcgctcg
     1621 agcttcgcct cctccgacca gatcctggtc atggccggcc gggagtagcg ctcgatcact
     1681 gaaccgattc tagatgcgcg ggcggcgcgt cacggcgccg tggggacaat cgtgctcgat
     1741 ttctccccgc tttcggcacg tttcggcgtg accttcgcgc ataggatcga agtgggtggt
     1801 gggctggggt gccccggggc aatctcgctg caagcgcacc tccgacgcgc cgtcagccgg
     1861 aaagagacac ggtcattccg ccggcggccc gctcggcgat gtccgtgcga taccgggccc
     1921 ccggaaacga gacgcgctcg atcgcggcgt aggcgagctc gcgtgccgcg tcgatcgtcc
     1981 ccccgcggcc gacgacgttc agcacgcggc caccgttcgt ggtcactcgt ccgtcatgga
     2041 gcgccgttcc ggcatggaag acgagggcgc cggccgcttc cgccgcctcg atgccggcga
     2101 tctccgaccc gcggtcggat tccgccgggt agccgcccgc cgcgagaacg acggcaacgg
     2161 aggcgccgtc gcttcgcccg agggagacac cggcgagctc gcctgccgca gccgccgcca
     2221 gcgcctcgag caggtcgccg ccgacgagcg gcagcaccga ttgggtctcg gggtcgccga
     2281 aacggcagtt gaactcgagc accttcgggc cctcggctgt cacgatcaac cctgcgtaga
     2341 gacacccgac aaacggctcg ccgcggtcgg cgagctcgtc gagcaccggt cgatggacgc
     2401 gatcgagcag cacctcgatg tcggcgttgc tgacgccggg aacgggggcg tacgagccca
     2461 tgcctccggt gttcggcccc tcgtcgccgt cgaacgcgcg cttgaagtcc tgcgcgggag
     2521 acagcccgag gagctcgcgg ccgtcacaca gcgcgaacag ggagagctcg gggccgacga
     2581 gcagctcctc gagcacgaca ggccccccga atgcggcggc ggcgcgcaga ccggcgtcca
     2641 gctcggcctg tgtttcgcac acgaagactc ccttgccggc ggcgagcccg tcagccttca
     2701 cgacgcaagg cgcccttgca acgctcagcc ggctcgccgt cggcacgaaa gacgcctcca
     2761 tcacgtcctt ggcgaagctc ttcgacccct cgatcctcgc cgccgcactg ctcggcccga
     2821 acacggcgat gcccgcgtgc cgcagcacgt ccgccacccc gccaaccagc ggcgcctccg
     2881 ggccgatcac gacgaggtcg gcggcgagcg agcgggcgag ggcgagcaga gcctcggcat
     2941 cttcggcgcc aaccggatga cagtgcccca gcgtcgcgat gcccggattg ccgggtgccg
     3001 cgtgaagctc ggagagtgac ggcgagctcg acagcttcca cgccagcgca tgctcgcggc
     3061 cgcccgagcc aacgatgagg acgatcaccg cggcgagtct agtgagcgtc cgggacgacc
     3121 cctgctcctg gcagcctggg ctgcgtcgca gccgcactct gcgagaatcc gcgcgtgttc
     3181 agcgccgcgg acgcatacgg tcgccatgtg ggccggtacg gggaggcgct cgcccgctcc
     3241 cacgcgaccg ctgccggcgt cagcgctggg cagcgcgccc tcgacgtcgg gtgcggccct
     3301 ggcgcattca ccgccgttct ggcggacatc ctcggccccg agaacgtcgc cgcggtagac
     3361 ccgtcggagc cgtttgccga gacctgccgg caacgcgtcc ccggcgcgga cgtccgggtc
     3421 gccccagccg aggagctgcc agacttcggc ttccgcttcg acgtcgtctc gtcccagctc
     3481 gtcgtcaact tcatgaccga cgcccgtgcg ggcatccgcg agatgtcgtc cagggctcgc
     3541 gccggcggaa ccgttgcctc ggtggtctgg gactacgcgg ccggcatgac gatgctgcgg
     3601 gcattctgga acgccgccgt cgcgctcgac cccgaggccc ctgacgaagg acgcacgatg
     3661 gcgtggtgca cgcccggtga gctccgcgag ctctggtcga ccgcgggcct cgagtccgtg
     3721 gagacacggc cgctgatcgt cgaggccgcc tacgagagct tcgctgactt ctgggatccc
     3781 ttcccgagcg gcatcggccc tacgggcgcc tactgcgccg cgctcgatgc cgagcatcgc
     3841 gagagcctcc gtcgggaatg cttccgtgag ctcggctcac cggcaggtgc gttcactctc
     3901 accgcgcggg cctggttcgt cggcggcacc gccgcgggct agacgttcgc ggcgtcaggg
     3961 aagcgacagc acagcttctc ggccggcgcc ggtccctacc agggtcaccg ggacgccgag
     4021 ggcgctctcc acgtagccga cgtaggagcg cgccgccgcg ggaagcgtct cgccggagag
     4081 ctcgtgctcc cagccggaaa gcgtctcgaa gaccggcgca cagtggtgga agtcgctctg
     4141 gtgggcgggg aactcctcgg tctcgctgcc gtcggggagc cggtagcgga cgcacaccgg
     4201 cagctccgcg aaactcgaaa gcacgtcgag cttcgtcagc gccagcgctg tgatgccgtt
     4261 cacccgcacc gcgaagcgca gcgcgaccag gtcgagccag ccgcagcgcc gttcccgccc
     4321 cgtgacggtg ccgaactcgc cgcccagctg acgtacgcgc tcctgcgctt cgccttggat
     4381 ctcgctcggg aagggcccct cgccgacccg ggtgacgtag gccttcgtga cgccgagaac
     4441 ctcatcgatc cgaccggggc cgattccgaa gctgaccgcc gcgcccgcgg caatcgggct
     4501 cgacgacgtg acgaaggga
//
LOCUS       DBNGNK010000213         8071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_610975,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000213 DBNGNK010000000
VERSION     DBNGNK010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_610975"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="AC150V_06275"
     CDS             <1..599
                     /locus_tag="AC150V_06275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR3762563.1"
                     /translation="VGDIVMAIGNPLGLRSSVTQGIVSALGRTVTTEGGSLPDTIQTS
                     APINPGNSGGALVNLSSRVVGIPTATATDPNFGAAAPGLGFAISSNRAKLIADQFIKD
                     GRVTQTDRAYLGVQVGDVQRDGVFVAGVEPRGPSARAGMREGDLIVRVAGKPTPSSVA
                     LAEVLATLRPGRRVAVRVARQDGSQKTLQVTLGELPGS"
     gene            725..1642
                     /locus_tag="AC150V_06280"
     CDS             725..1642
                     /locus_tag="AC150V_06280"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013476669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR3762564.1"
                     /translation="MRRLLLLLAGVALLAGLSVSSASAKAFPEIIPLPDGWQPEGIAV
                     GRGSTFYAGSLANGAVYRGDLRTGEGRILVAGGPGPAVGLAVDKRSNSLFVAGGPNGD
                     ARVYDAASGEEIATFALGPGFINDVIVTRDAAYFTNSFAPVLYRVPLGPGGGVDPTVA
                     PEPIPLSGDWTQLGGFNANGIEATPDGKWLIVVNSTAGEIYRVDPKTGAASEIDLGGA
                     SMVSSGDGLALLGKTLYVVRNFLNEIAVVDLAPSLLSGTVSRTITNPAFDIPTTVAPF
                     GSSLYAVNARFGTPPGPDVEYDVVKVSRK"
     gene            complement(1747..2124)
                     /locus_tag="AC150V_06285"
     CDS             complement(1747..2124)
                     /locus_tag="AC150V_06285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3762565.1"
                     /translation="MDSQITVVYRWTAKPGKMDELRAVYESVAEHSEANEPGITWMDC
                     YEAQGSDALIIQEVFEDGAALGAHLGGVAAEHFPKLSAIADFGPFFLCGDVPDELVQA
                     ASGMGATIATRAFGFSRSPSGTA"
     gene            2412..3227
                     /locus_tag="AC150V_06290"
     CDS             2412..3227
                     /locus_tag="AC150V_06290"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3762566.1"
                     /translation="MRRAVFDSFRRGFGREVLLAAGAMLLYFGVRNLSAGSAATALHN
                     AERLFALEERLGIAWEASAQAAIGGSQALITLANWVYIWGHWPVILPVAIVLFLLRRA
                     HYYLLRNAVFISGAIGFLFFALLPLAPPRLVDLGLLDTVTEQSSAYRALQPPGLTNQY
                     AAFPSLHAGWNLLVGIVLFLAFAHVAVRAFAILSPVAMAFAVVLTANHFVLDVAAGYA
                     VVLVGLAAAFMLDRRRRHAATLELDGVDRNGGKQRRLRENAAARHRAPRGQSA"
     gene            3277..3837
                     /locus_tag="AC150V_06295"
     CDS             3277..3837
                     /locus_tag="AC150V_06295"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3762567.1"
                     /translation="MRLFRGRAEIRHLKTIGPLPLYWDRWELDAPWRRPLFLSDLLEA
                     ADPGTELMLDLKGPRRRLAELVLDELRPHLGTRAFTVCARRWRLLDPFDGLPVTCVHS
                     VGSMRQLRLLVRRSGGRQIAAVAINERLLDAAVVQELRGLAELILTWPVNRVDRAAEL
                     VRLGVDGLITDDAEQITREEVLRALA"
     gene            3834..4739
                     /locus_tag="AC150V_06300"
     CDS             3834..4739
                     /locus_tag="AC150V_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3762568.1"
                     /translation="MSLLDHIAAALTAAGSRVAELDGRFLVPALALQLLALACRAFAW
                     RGILAAAYPEKRVPVFSVGAAYAAGVAMNAFLPLRGGEAVKVALARTRIPGSSVPTIA
                     ASLSVVLVLDMLIGVLLVTGLWSLGILPALPALPLDKLPLALGVLLLVASAGLLAAQT
                     RPSLVRPLAARLARGLAVLRSPGRYLFAVVPFQLAAWACRIGVVFFLLHAFGFHVGPE
                     TAALVVVLTGVSTAVPVPGGAGSQQVLATYALAGVASTAGAVSFSVGMQVGVTVVNTT
                     LGLVAAMLLFRTIRPLAAIRQARGA"
     gene            4835..5986
                     /locus_tag="AC150V_06305"
     CDS             4835..5986
                     /locus_tag="AC150V_06305"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR3762569.1"
                     /translation="MILTTFRRGMELCALVLLVALVVALATAAAPRLFGYRTVVVMSG
                     SMGDKAPVGSLVLGKSKDAGDVAVGDVIVANLNNSSLGTLHRVISVAQEDGSVVVQTK
                     GDLNPEPDPQHSTLPQQVFSMKYAIPYLGYLVWFSKKPLGWLFLVGIPALLIAGLVLR
                     GVWAPGRSAPDHQEAQPEQGTLLEQLRARQGHQPVLSPVPEGNDAESADVAPDGPPAP
                     VPEQIILLEQRFDELEHLLRSVGERSRDVEQYEAELVAADAALQEQQASLAVGAPALA
                     AADVPTVTRLVERAAPAPPRAELVVAHLQFIQTPQGYLMAEQPGPPPQDGMRVPNELD
                     PAVPYVVVRVGRSPLPDDPRPCAFLQLVGPQRPGSATAKEHERALVAVA"
     gene            6136..7119
                     /locus_tag="AC150V_06310"
     CDS             6136..7119
                     /locus_tag="AC150V_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762570.1"
                     /translation="MRRLRTEPPGELPPDEDDDRRRWLVLLLWLLVALLACLLLFVTF
                     RVVPFGGSDVGEPVGRGSAPSQASPGPSSAPGGSPGAPGRSSIPAGGSGVGRPAGGSD
                     RASVPEYGFSTPGQTTDAVTDSRPSGGSGSSSGRPGAAGERSVPADGSKPKPPPKEKP
                     AAAPPPAARESIIVVDPSKALTTFSNMAPGAKVTNALTVENRGSEPLRYGLLSVTTDR
                     RLAAQLVLTIKTGVVTCTNASFASSGTRVYGPGPLGSPEGTVLIGDGFNGAGKPLTLT
                     ADGRHELCFQVKLPIATGNDYQAATTVASYCVLPAGRIPTPQEISAGTRRC"
     gene            7245..>8071
                     /locus_tag="AC150V_06315"
     CDS             7245..>8071
                     /locus_tag="AC150V_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762571.1"
                     /translation="MRQLVPAAVILAVAVAALTVPVSRVLAQLVDSDSVPANAFSTNA
                     WTSVQTGVWSSTANGTFTVPISSVDPAKSFLVFSTASNSNRPVGTIVRGRILNATTLE
                     FVRVTDSVAPEPATINVRWYVASFASGVSVQRGQTTISTANQVDVPITPVASTGQAFV
                     LWSKTASPGDTSWGGNDVILGDLTSTSNLQFRYDQTGTHDVFWQVVQFTNSAAIGVQR
                     GSTSLMGGALSTTATLPTPVDVTKTFVLIGSRSAGGGNDVGSRMLRARLTSSTQITI"
BASE COUNT         1127 a         2868 c         2714 g         1362 t
ORIGIN      
        1 gcgttgggga catcgtgatg gcgatcggga atcccctcgg gctgcgctcg agtgtgacgc
       61 agggcatcgt gagcgcgctc gggcgcacgg tcacgaccga gggtggctcg ctgccggaca
      121 cgatccagac cagcgccccg atcaatccgg gaaacagcgg cggcgcgctt gtcaatctct
      181 cgagccgtgt cgtcggcatc cccaccgcga cggcgaccga tccgaacttc ggcgcggctg
      241 cgcccggcct cggcttcgcg atctcctcga accgggcgaa gctgatcgcc gaccagttca
      301 tcaaggacgg gcgggtcacg cagaccgacc gcgcgtacct cggtgtccag gtcggcgacg
      361 ttcagcgtga cggggtcttc gttgccggcg tggagccgcg cggaccgtcc gcaagggcgg
      421 gcatgcgcga gggggatctg atcgtcaggg ttgcaggaaa gccgaccccg tcctccgtcg
      481 cgctggcgga ggtgctcgcg acattgcggc cgggccggcg cgtcgccgtc cgcgtcgcca
      541 gacaggacgg ctcgcagaag acgcttcagg tgacgctggg cgagcttccc ggctcctagt
      601 ccagcgaggc gcctcgagcg gttgccggtt ggccggagcg aggttgcgca gactttcgcc
      661 ggtgtgaaac gctccaggaa gagcgcgcat atggcgcgct catctacctc gagggggttc
      721 tcaagtgaga cgattgctat tgctgctcgc tggcgttgca ctgctggctg gtctgtcggt
      781 ctcgagcgct tcggcgaagg cgtttccgga gatcattccg ctgccggacg gctggcagcc
      841 cgagggtatt gcggttgggc gaggatcgac cttctacgcg ggatcgctcg cgaacggcgc
      901 cgtctaccgc ggcgacctgc gcaccggcga gggccgcatt ctcgtggccg gagggcccgg
      961 ccctgcggtc ggcctggccg tcgacaagcg cagcaactct ctgttcgtag ccggcggccc
     1021 caatggggac gcgagggtgt acgacgccgc ctcgggggag gagatcgcga cgttcgcgct
     1081 cggccccggg ttcatcaacg acgtgatcgt gacgcgcgac gccgcctact tcacgaactc
     1141 gttcgcgccc gtcttgtatc gggtgccgtt ggggccgggt ggcggcgtcg atccgacggt
     1201 cgcgccggag ccgatcccct tgagcggcga ctggactcaa ctcggcggct tcaacgcgaa
     1261 cgggatcgag gcgacgccgg acggcaagtg gttgatcgtg gtcaactcga cggctggcga
     1321 gatctaccgc gtggacccga agaccggcgc tgccagcgag atcgacctcg gcggcgcgtc
     1381 gatggtgagc tccggtgacg ggctcgcgct cctcggcaag accctgtatg tcgtgcgcaa
     1441 cttcctcaac gaaatcgcgg tggtcgacct cgctccaagt ctgctctcgg gcaccgtgtc
     1501 ccgaaccatc acgaacccgg ccttcgacat tccgacgacg gtcgccccgt tcgggagctc
     1561 gctctacgcg gtcaacgccc gcttcggtac gccgccgggg cccgacgtcg agtacgacgt
     1621 cgtcaaggtg tcacggaagt aagccgcctt cggggccgct agcacacttt cagcgagccg
     1681 agccggggcg accaccttgt cggcccggct cggctccaga cttcgcggtt cccactcggg
     1741 cccggtctag gcggttccgg acgggctgcg cgagaagccg aaggcgcggg ttgcgatcgt
     1801 ggcccccatg cccgacgccg cctgcaccag ctcgtccggc acgtcgccgc agaggaagaa
     1861 gggcccgaag tcggcgatcg ccgacagctt gggaaagtgc tctgcggcca ctccgccgag
     1921 gtgagccccg agcgccgcgc cgtcctcgaa cacctcctgg atgatcaggg cgtcggatcc
     1981 ctgcgcctca tagcagtcca tccaggtgat gcccggctcg ttcgcctccg agtgctcggc
     2041 tacggactcg tagacggctc tgagctcgtc catcttgccc ggcttggcgg tccacctgta
     2101 gacaacggtt atctgggagt ccaatgcgtt ccctcctcgc tagcaggggt ttcggcgtgt
     2161 aggtgtctct cgaatcgctg ctgtcgacta tggcagcacg tcgcgctcga tgcgtcatcg
     2221 tgccggtgga caggacgtcg cccgatcgcc gcccggcgaa attggcgccg tgctcgcggc
     2281 ctttcgccag agccgatccg cggctccccc gatgcgcgcc gagtgcgccc tcggcaaaga
     2341 tggatgggaa ggaggtgggg aagctggccg ccgagtcttt gccgcggcct ccgctccagg
     2401 atcggcgtgg cttgcgccgc gccgtctttg acagctttcg tcgcggcttc gggcgggagg
     2461 tgcttctcgc cgcgggcgca atgttgctgt atttcggcgt tcgcaacctc agcgccggca
     2521 gcgctgcgac tgccctgcac aacgccgagc gcctcttcgc cctcgaggag cggctcggga
     2581 tcgcctggga ggcaagcgcg caggctgcca tcggaggcag ccaagcgctc atcacgctgg
     2641 ccaactgggt ctacatctgg ggccattggc cggtgatcct ccccgtcgcg atcgtcctct
     2701 tcctgctccg gcgggcccac tactacctcc tccgcaacgc tgtcttcatc tcgggagcca
     2761 tcggcttcct cttcttcgcg ctcctccctc tggcgccgcc gcgcctggtc gatctcggtc
     2821 tcctcgacac cgtcaccgag cagtcgagcg cctaccgagc gctccagcct cctggcctga
     2881 cgaaccagta cgcagccttc cccagtctcc acgccggttg gaatctcctc gtcggcatcg
     2941 tgctgttcct ggccttcgcg cacgtcgccg tgcgcgcctt tgcgatcctc tccccggtgg
     3001 cgatggcgtt cgcggtcgtc ctgaccgcga accacttcgt gctcgacgtg gcggccgggt
     3061 acgcagtcgt cctggtcggc ctcgccgccg ccttcatgct ggatcgacgc cgccgccacg
     3121 cggctacact cgagctggat ggggtcgatc ggaacggcgg caagcaacgc cgcctcagag
     3181 agaacgccgc cgctcgtcat cgcgcaccgc gcgggcaatc ggcttgacgt ccttcgtcgc
     3241 gcagaggcca ccggtgctgc cctgatcgag gccgatgtgc gtctctttcg cggtcgcgcc
     3301 gagatccgcc acctcaagac gatcggcccg ctgccgctct actgggatcg gtgggagctc
     3361 gatgcgccgt ggcgccgccc gctcttcctc agcgacctgc tggaggcggc cgatcccggc
     3421 acggagctga tgctcgacct gaaggggccg cggcgccgcc tcgccgagct cgttctcgac
     3481 gagctgcggc ctcatctcgg cacccgcgcc ttcaccgtct gcgccaggcg ctggcgtctc
     3541 ctcgatccgt tcgacggcct tccggtcacc tgcgtgcact ccgtcgggag catgcgccag
     3601 ctgcgattgc tcgttcgccg gtcgggcggc agacagatcg cggcggtggc gatcaacgag
     3661 cgtctgctcg acgccgcggt cgtccaggag ctccgcgggc tcgcggagct gatcctgacg
     3721 tggccggtca accgcgtcga tcgcgccgcg gagctcgtcc gcctcggggt cgacgggctg
     3781 atcaccgacg acgctgagca gatcacccgc gaagaagtcc tccgggcgct ggcgtgagcc
     3841 tgctcgatca catcgcagcc gcgctgaccg cggccggcag ccgcgtcgcc gagctggacg
     3901 gccgtttcct ggtgcccgca ctcgcgctgc agcttctcgc gctcgcgtgc cgcgccttcg
     3961 cctggcgcgg cattctcgcc gccgcctacc cggaaaagcg cgtccccgtg ttctcggtcg
     4021 gtgctgcgta tgcggcaggc gtcgcgatga acgcgtttct gcccctgcga ggcggagagg
     4081 cggtcaaggt cgcgctcgcc cgcacgcgca tccccggttc ctcggtcccg acgatcgccg
     4141 cgtcgctcag cgtggtgctc gtgctggaca tgctcatcgg cgtcctgctc gtcacggggc
     4201 tctggtcgct cgggatcctg cctgcactgc ctgcgcttcc gctcgacaag cttcccttgg
     4261 cgctcggcgt gctcctgctc gtggcttcgg cgggattgct cgccgcccag acgcggccga
     4321 gcctcgtccg acctctcgcc gcacgcttgg cgcgcggcct cgccgtgctt cggtcgcccg
     4381 gacgctacct cttcgcggtt gttccgttcc agcttgccgc gtgggcgtgt cgtatcggcg
     4441 tcgtgttctt cctcctgcac gccttcgggt ttcacgtcgg gcccgagacg gccgcactgg
     4501 tcgtcgtact gacgggtgtc tcgacggccg ttccggttcc gggcggagcc gggtcgcagc
     4561 aggttctggc gacgtacgcg ctcgccggcg tcgcgtcgac ggcaggcgcc gtctcgttct
     4621 cggtcggcat gcaagtgggc gtcacagtcg tcaacacgac actcggcctg gtcgccgcga
     4681 tgctgctctt ccgcacgatt cgaccgctcg cggcgatccg gcaagcacgc ggcgcttgac
     4741 cagcgggcga gcggccgctg ccggccgccg aggaggcgga cgagaatcgc accgcgcgca
     4801 gggtctcggc gcttgcggtg gaaaagagca ctcgatgatc ctcacgactt tccgtcgggg
     4861 aatggagctg tgcgccctcg tcctcctggt cgcactcgtc gtcgcgctcg cgacggcggc
     4921 cgcaccgcgg ctgttcggct accgcacagt ggtcgtgatg agcggcagca tgggggacaa
     4981 ggccccggtc ggcagcctcg tgctcggaaa gtcgaaggac gccggggatg tcgcggtcgg
     5041 cgacgtgatc gtcgcgaatc tgaacaacag ctccctgggg acgcttcacc gcgtgatctc
     5101 cgtggcgcag gaggacggaa gcgtcgtcgt gcagacgaag ggcgatctca atcccgagcc
     5161 cgacccccag cactccacgc ttccgcagca ggtcttcagc atgaagtacg caatcccgta
     5221 tctcggctac ctcgtctggt tctcgaagaa gcccctcggc tggctgttcc tcgtcggcat
     5281 accggctctg ctgatcgcgg gactcgtcct cagaggcgtc tgggcaccgg gcaggtccgc
     5341 ccccgatcat caggaagctc aacccgagca ggggacgctg ctcgagcagc tgcgcgcaag
     5401 acaagggcat cagcctgtcc tgtcccccgt gccggagggg aacgatgccg agtccgccga
     5461 cgtcgcgccc gacggcccgc cggcgcccgt gcccgagcag atcatcctcc tcgagcagcg
     5521 gttcgacgag ctcgagcatc tgcttcgctc ggtgggagag cggagccgag acgtggagca
     5581 atacgaagcg gagctcgtag cggccgacgc tgcgctccaa gagcagcagg cgtcactcgc
     5641 agtcggcgcg ccagcgctcg cggcggccga cgtgccgacc gtcacgcgcc ttgtggagcg
     5701 ggcagcgccg gctccgccgc gagccgagct ggtcgtggcg cacctgcagt tcatacagac
     5761 gccgcagggc tatctgatgg ccgagcagcc ggggccgccc ccacaagacg ggatgcgcgt
     5821 tcccaacgag ctcgatccgg cagtgcccta cgtcgtcgtg cgggtcggtc gctctccgct
     5881 ccctgacgat ccgcgtccct gcgcgttcct gcagctcgtc ggaccgcaga ggccgggctc
     5941 cgcgacggcg aaggagcacg aacgggccct cgtcgcggtg gcctgagcca aacgtcctgg
     6001 cgcggtctct cagccgagcg tgtggctcag aaagcctcaa gaacggcggt ctcggcgccg
     6061 acgaccagtt gcatgggcac ctttcgatcg cccgacgacc ctcggttggg gccctcttcg
     6121 ccctgggaga cgctcgtgcg gcgccttcgc accgagccgc ccggggagct tcctcctgac
     6181 gaggacgatg atcgccgacg ctggcttgtc ctgctcctct ggttgctggt tgctctgctg
     6241 gcgtgcctgc tgctcttcgt gacgttcagg gtcgttccct tcggcgggtc ggatgtcggc
     6301 gagcctgtcg gccgcggttc cgcgccgtcg caggcatcgc ctggaccgtc cagcgctcct
     6361 ggcggaagcc ccggtgcgcc ggggcggtcg agcatccccg ccggcgggag cggcgtgggc
     6421 cggccggcag gtggctccga tcgagcaagc gttcccgagt acggattcag tacgcccggg
     6481 cagacgaccg acgcggtcac cgactcgcgc ccttccgggg gatcgggcag ctcctcgggc
     6541 cgaccgggcg ccgccggcga gcggagcgtc ccggcagatg ggtcgaagcc gaagcctcct
     6601 cccaaggaga agcccgctgc ggccccgccg cccgccgccc gcgagtcgat catcgtcgtc
     6661 gatccgtcga aggcgctcac gaccttctcg aacatggcgc ccggcgcaaa ggtgacgaac
     6721 gcgctcaccg tagagaacag gggcagcgaa ccgctccgct acggcctcct ctcggtcacg
     6781 accgaccggc ggctcgccgc tcagctcgtg ctgacgatca agacgggcgt cgtgacgtgc
     6841 acgaacgcga gcttcgcctc gagcggaacc cgcgtgtacg ggcccgggcc gctcggcagc
     6901 cccgagggca ccgtgctgat cggcgacggc ttcaacggcg ccggcaagcc gctgacgctc
     6961 accgccgacg ggcggcacga gctctgcttc caggtgaagc tcccgatcgc gaccggcaac
     7021 gactaccagg cagcgacgac cgttgcgtcc tattgcgtcc tacccgcggg ccggatccca
     7081 acaccgcagg agatctcggc cgggacgcgg cggtgctgaa gctgcgattc aagtagtcgg
     7141 gcccgtcgtc cgaggtagga cctggcgacc caccaccaga cgggatctcc atgcggcaca
     7201 accaccatcc gaacccgacg tcctccgcga cgaggtcgtc acgagtgcgg cagctcgtac
     7261 cggccgccgt catccttgct gtcgcggtcg cggctctcac cgtacccgtc tctcgcgtgc
     7321 tcgcacagct cgtcgactcg gacagcgtgc cggccaacgc gttctcgacg aacgcctgga
     7381 cgtccgtgca gacgggtgtc tggagcagca cggccaacgg cacgttcacg gttccgatct
     7441 cgtccgtcga tccggccaag tccttcctcg tcttcagcac cgccagcaac agcaaccgcc
     7501 ctgtcggcac gatcgtccgc gggcgcatcc tcaacgcgac gacgctcgag ttcgtgcgtg
     7561 tgacggattc ggtcgcgccg gagccggcca cgatcaacgt ccgttggtat gtcgccagct
     7621 tcgcctccgg cgtgagcgtc cagcgcggcc agacgacgat cagcaccgcc aaccaggtcg
     7681 acgtcccgat cacgccggtt gcgtcgacgg gccaggcgtt cgtgctctgg tcgaagacgg
     7741 cgtcaccggg cgatacgtcg tggggtggaa acgacgtcat cctgggcgac ctgacgagca
     7801 ccagcaacct ccagttccgc tacgaccaga ccggaaccca cgacgtcttc tggcaggtgg
     7861 ttcagttcac gaattccgcc gcgatcggcg tccagcgcgg ctcgacctcg ctgatgggtg
     7921 gagcgctctc cactaccgcc acgttgccga cgccggtcga cgtcacgaag acgttcgtct
     7981 tgatcgggtc ccggtcagca ggaggcggga acgacgtcgg atcacggatg ttgcgcgccc
     8041 gcctgacgag ctccacccag atcacgatcg a
//
LOCUS       DBNGNK010000214         4642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1471024,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000214 DBNGNK010000000
VERSION     DBNGNK010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1471024"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..532
                     /locus_tag="AC150V_06320"
     CDS             104..532
                     /locus_tag="AC150V_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762572.1"
                     /translation="MGLFSKRPQAPTSGDLRRERRALMLLREERLRDLGGLTLEMYRR
                     DDFNDVLIVERCAELVAVEARVSEIDAILAGARGLRKRASAVCDCGAPLLIGARYCPS
                     CGRRVVVDETEQGAAQTNGDASSGGTAAESRATKVGEAAE"
     gene            529..1089
                     /locus_tag="AC150V_06325"
     CDS             529..1089
                     /locus_tag="AC150V_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762573.1"
                     /translation="MTACPRCGSGVAATQEYCLDCGLRLPGPGRLGPAPVERRRLAAP
                     LIAAALIATAGAGAAIALTWDDSAAGRVLIATVGADTTAQAKKQPSQKPKLAAWPAGK
                     DGWTVVLLSVPKDEGRAAAVERADQATARGLKTVGIVDSSKVAGLHPGYWVVFSGIYD
                     TKPEATSELLQARTFAKSAATRKLSG"
     gene            1235..1684
                     /locus_tag="AC150V_06330"
     CDS             1235..1684
                     /locus_tag="AC150V_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762574.1"
                     /translation="MEEPAAQLQSCMYQYSRAIYRSIKDLIDPYADRQTRLEYRRAVL
                     SDCEETMSRLASDPQYFARPDRTLFQDIRRYFPITAQARVAWAVQEGVGAAVSFIEAQ
                     IEAGLLDGGEARCRATTRKGKPCQRTPLPSRDYCPSHQHLEQKAAAA"
     gene            1776..3812
                     /locus_tag="AC150V_06335"
     CDS             1776..3812
                     /locus_tag="AC150V_06335"
                     /inference="COORDINATES: protein motif:HMM:NF039255.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR3762575.1"
                     /translation="MSHDADKLIRQLSLVAFLMAERRPLTARDVKSNVEGYSEMSDEA
                     FARRFYSDRIELTSLGVPLHSQRDEFTGEELYTLRSENYFLDRLELEDEELAALQTAL
                     YYLEGKFAYAEPLRLALQNLALGRPGFPHAPTSTAERVRVSAPDYSPELAGRLSKLEA
                     AISKQRTIKFSYWSPQRDRVAERTLNPYALRLDEGNWYVVGQDLDRKAVRTFRVSRIR
                     GEIRFATRRERDFRLPEEFDVDEHRVPKPWQIGEPVGRARIAVAGDTAWWVHRTLADA
                     GTLTDGVFETDFARIEPLAGWVLRQNGRAVPLEPDELRETVAEGLARLADRHRGPAPT
                     LARERRVSARALADERTPGPVAPERFGILQALLAHVLAACGDGREADLDADELAQSFS
                     IPRAELQDHLSLLNLVNFGGGCYTVYAEVDEETGRVRVDKELYGDVFRRPPKLTPLEA
                     RAIRLAIEYVGPTIAAEAHTPLDRVRRKLEETFGQFELAQTPTPSVRDAEEGLVRTLS
                     TGADQRRIVEIEYLKEGDEAPYVREVEPYSFERELPVWRVHTWDRTVDAPRTYRLDRM
                     RSARMTRRRFEPRDGFDPNYMAEPSLAMLWHSPLVARYRIERGARPLTDKAAVTELPF
                     KTEEWLLSEVLADRGETVVLEPTPLRAVVAKRAKELIGELDVKPARRSRSAERA"
     gene            complement(3772..4419)
                     /locus_tag="AC150V_06340"
     CDS             complement(3772..4419)
                     /locus_tag="AC150V_06340"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3762576.1"
                     /translation="MSRPETLSLLAHELRSPVAALTAIADALASRRVTISSEDRARLL
                     RLAVEAARDIERIVLDATPDSLRLETVEPAQLVRDIVDGARLTGALVRLETEEELPTL
                     QADPVRLRQALANLVANAAAHSPPGTDVVIAARLVRRSVRIEVTDFGEGVAAADQGRI
                     FEPGVRLVDRPGRGLGLAVALAVAEAHGGTVEVQSAPGEGATFTLVLPSGSAAQA"
     gene            complement(4416..>4642)
                     /locus_tag="AC150V_06345"
     CDS             complement(4416..>4642)
                     /locus_tag="AC150V_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3762577.1"
                     /translation="AEWLTAGDEELIERYERLYVPRNDPRWLRRNALIALGNVGGRAD
                     TPLIERYAEDDDEMLAETAEWALAQIAERT"
BASE COUNT          742 a         1639 c         1581 g          680 t
ORIGIN      
        1 gggcaacacg cgggaaggcc ccgcgtgggc ggtgcgcgag atgaccgagg agcgcctcgt
       61 cgtcctgcag ctctgactga gccggcgagt atcctcagcg gggatgggcc tcttctccaa
      121 gcgcccgcag gcgccgacct ccggcgatct ccgccgtgag cgacgcgccc tcatgctgct
      181 gcgcgaggag cgcctgcgcg atctgggcgg cctgacgctc gagatgtacc ggcgggacga
      241 cttcaacgac gtcctcatcg tcgagcgttg cgcggagctc gtcgcggtcg aggcccgtgt
      301 gagcgagatc gacgcgatcc tcgcgggggc gaggggcctg cggaaacgcg ccagcgcggt
      361 ctgtgactgc ggcgcgccgc tcctgatcgg cgcgcggtac tgcccgagct gcggccgccg
      421 cgtcgtcgtg gacgagaccg agcagggagc cgcgcagacc aacggcgacg cctcgtccgg
      481 cggcacggcg gccgagtccc gggcaaccaa ggtgggagaa gcggccgagt gacggcgtgc
      541 ccgcgctgcg ggtcgggcgt cgcggccacg caggagtact gcctcgactg cgggctgcgc
      601 cttcccggac ccggacggct cgggcccgcg cctgtcgagc gccgtcggct cgctgccccg
      661 ctgatcgcgg ctgccctgat cgcgaccgcc ggggcgggag cggccatagc gctcacctgg
      721 gacgactccg ccgccgggcg cgtgctgatc gcgacggtgg gggccgatac gacggcgcag
      781 gcgaagaagc agccaagtca gaagccgaag cttgcggcct ggcccgccgg caaggacggc
      841 tggacggtcg tgctcctttc cgtcccgaag gacgagggac gagccgccgc cgtggagcgt
      901 gccgaccaag caacggcgcg cgggctgaag acggtcggga tcgtcgactc gagcaaggtt
      961 gcaggcctcc acccgggcta ctgggtggtg ttctccggta tctacgacac gaagccggag
     1021 gccaccagcg agctgttgca agcgcgaacc ttcgccaagt ctgcggcgac gcgaaagctc
     1081 tcgggctgag caagcagccg ttttcttatc ggaaaacgtt agtgcgggac tttgtaacaa
     1141 acccgttaca cgctacaatg gcggcgatcc cgacgtccct caccgttctt atcgcctgag
     1201 agcgaccttt tcacgagagc gagacgagcc gaaaatggaa gagcccgccg cccaactgca
     1261 gtcttgtatg tatcaatact cgcgagcgat ctaccgctcg atcaaggatc tcatcgaccc
     1321 ctacgccgac cggcagaccc gcctcgagta ccggagggct gtcttgtccg actgcgagga
     1381 gacgatgtcg cggttggcat cggatccgca gtacttcgcg cgacccgatc ggacgctctt
     1441 ccaggacatc cggcgctact tcccgatcac cgcgcaggct cgcgtcgcct gggccgtcca
     1501 ggagggcgtc ggcgcagcag tctccttcat cgaggcgcag atcgaggccg ggctgctgga
     1561 cggcggcgag gcccgctgcc gagccacgac gcgaaagggc aagccctgtc agcgcacgcc
     1621 cctgccgagt cgcgactact gcccgtcaca ccagcacctc gaacaaaagg ccgctgccgc
     1681 ctgactcgcg ccccagcgcc ggaacggcca caacggacgc gcccccattc ggcggcccgc
     1741 cgacggctcg gcgtcgagta agctcgcctt cgtccgtgtc acacgatgcc gacaagctga
     1801 tccgccagct ttcgctggtc gcgttcctga tggcggagcg gcgcccgctg accgcccgcg
     1861 atgtgaagtc gaacgtcgag ggctactcgg agatgtccga cgaggccttc gcgcgtcgct
     1921 tctactccga tcggatcgag ctgacgtcgc tcggcgttcc gctccactcc cagcgtgacg
     1981 agttcacggg ggaggagctc tacacgcttc gctcggagaa ctacttcctg gatcggctag
     2041 agctcgagga cgaggagctc gcggctctgc agaccgctct ctactacctg gaagggaagt
     2101 tcgcctacgc cgagccgctc cggctcgcgc tccagaacct ggcactcggc cgccccggat
     2161 ttccccatgc gccgacgtcg acagccgagc gcgttcgcgt cagcgcgccc gactactcgc
     2221 ccgagctggc cgggcgcctc tcgaagctcg aggctgcgat ctccaagcag cggacgatca
     2281 agttctcgta ctggtcgccc cagcgcgacc gcgtcgcgga gcggacgctc aatccctacg
     2341 cgctcaggct cgacgaaggc aactggtacg tcgtcggtca ggacctcgac cggaaggcgg
     2401 ttcgcacctt ccgcgtctcc cggatccgag gggagatccg cttcgcgaca cgacgcgagc
     2461 gcgactttcg tctcccggag gagttcgacg tggacgagca tcgcgtgccg aagccgtggc
     2521 agatcggcga accggtcggc cgcgcgcgca tcgccgtggc cggtgacacc gcttggtggg
     2581 tgcatcgaac cttggccgat gccggcacgc tcaccgacgg cgtcttcgag acggactttg
     2641 cgcgcatcga gccgctcgcc ggctgggtgt tgcgtcagaa cggcagagcg gttccgctcg
     2701 agccggacga gctccgggaa acggtagccg aaggcttggc ccgtcttgcc gaccgccacc
     2761 gcggccccgc gccgacactc gcccgggagc gacgcgtcag cgcccgcgct ctcgccgacg
     2821 agcggacccc ggggccggtc gctcccgagc ggttcgggat cctccaagcg cttctcgctc
     2881 acgtgctcgc agcctgcggc gacgggcgcg aggccgacct cgacgccgac gagctcgcac
     2941 agagcttctc catcccccgc gccgagctgc aggatcacct ctcgttgctc aatctcgtca
     3001 acttcggcgg cggctgctac acggtctacg ccgaggtcga cgaggagaca ggacgcgtcc
     3061 gcgtcgacaa ggagctgtac ggggacgtct tccgcaggcc cccgaagctc acgccgctcg
     3121 aggctcgcgc gatccgcctg gcgatcgagt acgtcgggcc cacgatcgct gccgaggctc
     3181 atacgccgct cgaccgcgtc cggcgaaagc tcgaggaaac cttcggtcag ttcgagctcg
     3241 cacagactcc gacgccgagc gtccgggacg ccgaggaggg tctcgtgcgc acgctgagca
     3301 cgggtgcaga tcagcgacgc atcgtcgaga tcgaatacct gaaggaaggc gacgaggcgc
     3361 cgtacgtccg cgaggtcgag ccgtactcct tcgaacgtga gctccccgtc tggcgcgtac
     3421 acacgtggga tcgaaccgtc gacgcgcccc gtacctaccg gctcgaccga atgcgctcgg
     3481 cgcggatgac gcgccgccgc ttcgagccgc gtgacggctt cgaccccaac tacatggccg
     3541 agccgagcct cgccatgctc tggcattcgc cgctcgtcgc ccggtaccgg atcgagcggg
     3601 gcgctcgccc gctcaccgac aaggctgcgg tcacggagct ccccttcaag acggaggagt
     3661 ggctcctgag cgaagtgctc gccgaccgcg gcgagaccgt agtgctggag ccaacgccgc
     3721 tgcgcgcagt cgtcgccaag agagcgaagg agctgatcgg ggagctggac gtcaagcctg
     3781 cgcggcggag ccgctcggca gaacgagcgt gaaggtggcg ccctccccgg gcgccgattg
     3841 cacctcgacc gtcccgccgt gcgcctcggc caccgccaga gcgacggcga ggccgagtcc
     3901 cctccccggc ctgtcaacga gccgcacgcc cggctcgaag atccgtccct ggtctgcggc
     3961 cgctactccc tcaccgaagt cggtgacctc gattcgcacc gagcgccgga cgaggcgcgc
     4021 ggcgatcacg acgtccgtgc cggggggaga gtgcgcggcg gcgttcgcga cgaggttcgc
     4081 aagagcctgg cgcaaccgga ccggatcagc ctgaagcgtc ggaagctcct cttctgtctc
     4141 gagccgcacg agagcccccg tcagccgggc accgtcgacg atgtcacgca cgagctgagc
     4201 cggctccacc gtctccaggc gcagcgagtc gggcgtcgcg tcgaggacga tgcgctcgat
     4261 gtcacgggcc gcctcgaccg cgagccgaag caggcgggct ctgtcctcgg acgagatcgt
     4321 cacgcgccgg gaggcgagcg cgtcggcgat cgcggtcagg gcggcaacgg gactgcgcag
     4381 ctcgtgtgcg agcagagaga gcgtctcggg acggctcatg ttcgctccgc gatctgagcc
     4441 agtgcccatt ccgccgtctc ggccagcatc tcgtcatcgt cctccgcata gcgctcgatg
     4501 aggggcgtgt ccgcgcggcc gccgacgttg ccgagcgcga tcaacgcgtt tcgccgaagc
     4561 caacgcggat cgttgcgggg gacgtagagc cgctcgtagc gttcgatcag ttcctcatct
     4621 ccggcggtga gccactcggc aa
//
LOCUS       DBNGNK010000215         7857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1471125,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000215 DBNGNK010000000
VERSION     DBNGNK010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7857
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1471125"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /gene="truB"
                     /locus_tag="AC150V_06350"
     CDS             complement(<1..398)
                     /gene="truB"
                     /locus_tag="AC150V_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013660.7"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR3762578.1"
                     /translation="MPPRGLDPTGIIFVDKPAGPSSFAVVDRVRRRTGARTGHAGTLD
                     PFATGLLLLLSGRATKLASSFMGLDKRYVAEIDLRASTSTGDPEGEVADEQEPPSPAQ
                     LEEDLAALRGDVELPIPAASAVKIAGERSYR"
     gene            complement(409..1443)
                     /locus_tag="AC150V_06355"
     CDS             complement(409..1443)
                     /locus_tag="AC150V_06355"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR3762579.1"
                     /translation="MTHENEMGAVVAALRARDRFVVATHESPDGDALGSLLAAHLVLG
                     RLGKDSVMFLSGQSQLPGEYRFLQLDDVRRELPDDLGERTLLAVDCAQESRLGPESAA
                     LLEAVPFTINVDHHHDNTLFGDVDLVVPAASSTGEVLADVMEELGVALTPELAEPLYT
                     AVVTDTGRFQYANTTPKALRLAADLIEAGADAHKVFQVVYESVQFAKLKLLARALERA
                     EVYEGGRVVVSHLRRIDFVDAGAAEPYSEGIIDYLRAVEGAELAVLVREQVEGKAHAW
                     KVSLRSSVDEIDVSTIARTFGGGGHPQAAGLSSDLPPEEITSQILREFEQIASASEPA
                     ARHDPVADRP"
     gene            complement(1427..1777)
                     /gene="rbfA"
                     /locus_tag="AC150V_06360"
     CDS             complement(1427..1777)
                     /gene="rbfA"
                     /locus_tag="AC150V_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR3762580.1"
                     /translation="MRRVDEAIREVLSEAIPTLKDPRIGFVTVTGVKTTTDIKQATVW
                     LSVLGSEQERTATLRALEGAAGVLQSELNRQLHLRRTPQLVFAYDPSVERGVRITKLI
                     DELAPGEAPDDPRE"
     gene            complement(1782..2081)
                     /locus_tag="AC150V_06365"
     CDS             complement(1782..2081)
                     /locus_tag="AC150V_06365"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR3762581.1"
                     /translation="MATGYVGIYAFELFIPESRSLKDKRMVVRSVKDRLSNRVGCSIA
                     EVEHHDVWQRAGLTLACVAREAGETERLLDEAERWLSGQHFELMQARRGVVTLDE"
     gene            complement(2102..3940)
                     /gene="infB"
                     /locus_tag="AC150V_06370"
     CDS             complement(2102..3940)
                     /gene="infB"
                     /locus_tag="AC150V_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR3762582.1"
                     /translation="MPAQPKQPQQPQQPTGPVIVESGVTVKDLSQALGVPMPQIIKTL
                     MGLGQMRTATQSLSDEEVELIAAEVSREVEIKHASEEDLEPIAVEDAESDLEPRPPVV
                     TIMGHVDHGKTTLLDAIRQTAVVETEAGGITQHIGAYQAEVDDRRITFLDTPGHEAFT
                     AMRARGAKVTDIAVLVVAADDGVMPQTRESISHARAAEVPIVVAVNKIDVADANTDRV
                     KGELANEGLQPEDWGGTTQFSEVSAKEQQNLDDLLERILLVADAELDLRANPNAEASG
                     PIIESRLDVGRGPVATMLVQRGTLKVGDAIVAGDAAGKVRALHDYFGEKVTEAGPGDP
                     VEILGFDKPPPAGELARVVEHERQAKHLANLRAERLRREQLAQRSARGVSLETLFDQL
                     QEGAVQVLNIVLKGDVQGSIEALVGELGKIQHSEVRVNVIHTGVGGITENDVNLASAS
                     NALVVGFNVRPSAEARGLAERVGVDIRTYRVIYQLTEDIQKALVGMLSPVETEETLGE
                     AEVRALFKVSRLGTIAGCMVTDGAIRRGALVRVVRDGTVVQDTAITQLKRFKDDVREV
                     SEGFECGILLDGFNDVKEGDVLEAYERRAVERTELDEPAAAATPGE"
     gene            complement(4076..4315)
                     /locus_tag="AC150V_06375"
     CDS             complement(4076..4315)
                     /locus_tag="AC150V_06375"
                     /inference="COORDINATES: protein motif:HMM:NF016204.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HIR3762583.1"
                     /translation="MSPEPERTCVGCGRRAPQAELIRFAAVDGVLTPGRTLPGRGAYT
                     CRRRSCFERATSARAFNRVLRRSVKVESTLARLYT"
     gene            complement(4312..5769)
                     /gene="nusA"
                     /locus_tag="AC150V_06380"
     CDS             complement(4312..5769)
                     /gene="nusA"
                     /locus_tag="AC150V_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR3762584.1"
                     /translation="MSIEIIENIRALESEKGIEQDTLVHALEDALLAAYKKTPGAARH
                     AEVILDDEGEFRVFAIDIPADLEERLLDEAREEAIAELERVEEETGERQHTLISDDDL
                     DLDWSQVPDAQMVRTDVTPGDFGRIAAMTAKQVIQQRIREAERAIRYAEYIDRVGDVV
                     TGIVQQAGDRNNVLVDLGNNVEALLPRSEQVDGERVEQGGRIKAVIIEVRSGTKGPQV
                     ILSRRDPELIKTLFELEVPEIADGLVEIRGVAREPGYRAKIAVESHVQGVDPVGACVG
                     PRGSRVRMVVSELRGEKIDIIPWNTEPARFVAKALSPARVREVYIDDETKDATVVVPD
                     DQLALAIGKEGLNARLAARLTGWKIDIQSDTEFAQAEADAAFAGDDDTEEFSGRCAAI
                     LSNGKRCPNAALPASKYCGVPAHQALVGQESPVELTTAVEVMVEDAASGLVTEELEAE
                     VVAEAEEIVEDAADPGQPVAEEATEEAPSGEATGG"
     gene            complement(5779..6219)
                     /locus_tag="AC150V_06385"
     CDS             complement(5779..6219)
                     /locus_tag="AC150V_06385"
                     /inference="COORDINATES: protein motif:HMM:NF036577.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR3762585.1"
                     /translation="MANAYATERELERDITPAVQGRLPDVEVLAVELLSPTRFCVYVD
                     HPEGVDHALCERVTRLLDGYRVDYTIDVSSPGPERPVRTAAHFERALGRSVAIRTENE
                     IGGTRRFRGVIAAAGRDHATIRTAEGELDIPYEAIVRGNLIDDG"
     gene            complement(6339..6719)
                     /locus_tag="AC150V_06390"
     CDS             complement(6339..6719)
                     /locus_tag="AC150V_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3762586.1"
                     /translation="MPQLSAVGVVVSDMAETIRFYRLLGLDMPETPGVGHVETVFPNG
                     CRLMLDTEEVIRSFRPEWVRESGNQLALAFECARPADVDEIYARVTDAGFTGDKSPWD
                     AFWGQRYAQLRDPDGVPVDLYASL"
     gene            6954..7397
                     /locus_tag="AC150V_06395"
     CDS             6954..7397
                     /locus_tag="AC150V_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019135661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR3762587.1"
                     /translation="MKRKLLLSIGRKPRKTADELLREARTRLRRMTPREALAAVSRGA
                     LLIDLRSGDDRRRDGIIPDSLHIPRTVLEWRADPASDYADPEIGDDLGRTLILVCTDG
                     YSSSLAAATLQDLGFAAATDVIGGYRAWEEAGLPTRLSAEMTRVI"
     gene            complement(7425..>7857)
                     /locus_tag="AC150V_06400"
     CDS             complement(7425..>7857)
                     /locus_tag="AC150V_06400"
                     /inference="COORDINATES: protein motif:HMM:NF013593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR3762588.1"
                     /translation="MIMRFGMSEKLGPRVLGRDSNQPFLGREMSAEPDYSDEVAKEID
                     DEIRRVIEEAHDMAANVLRERIEELHMLSGILIERETIDKDQFERLLAGEAEESVFAE
                     EAKPVEEPEEEPEREPKPALQPQPRPLPGAAMQPPPEGAAG"
BASE COUNT         1172 a         2774 c         2546 g         1365 t
ORIGIN      
        1 cggtaggagc gctcaccggc gatcttcacg gccgacgccg ccgggatggg aagctcgacg
       61 tcaccgcgta gcgcggcgag atcctcctcg agctgcgccg gggacggcgg ctcctgctcg
      121 tcggcgacct ccccctccgg atcgccggtc gaggtgctgg ctcgcaggtc tatctcggcc
      181 acgtagcgct tgtcgagacc catgaacgag gacgcgagct tcgtcgcccg ccccgagagc
      241 agtagcaaca gtccggtcgc aaacggatcg agggttcccg cgtggccggt gcgggcacca
      301 gtccggcggc gcacccggtc gaccaccgcg aacgacgacg gccccgccgg cttgtcgacg
      361 aagatgatcc cagtcgggtc gaggcctcgc ggcggcatcg cttcgctgtc agggccggtc
      421 ggcgaccgga tcgtgccgcg ctgccggctc ggatgccgag gcgatctgct cgaactcgcg
      481 caggatctgg ctcgtgatct cctccggcgg aaggtcgctc gacaagcccg ccgcctgcgg
      541 atgcccaccg ccaccgaacg tcctggcgat cgtcgagacg tcgatctcgt cgacggagga
      601 gcgaagcgag accttccagg cgtgcgcctt cccttcgacc tgctctctga ccaagaccgc
      661 gagctcggcc ccctccaccg cccgcaggta gtcgatgatc ccctccgagt acggctcagc
      721 cgctcccgcg tcgacgaagt cgatccgccg caggtgggag acgacgacgc ggccgccttc
      781 gtacacctcg gcacgctcga gcgctcgcgc gagcagcttc agcttcgcga actgcacgga
      841 ctcgtagacg acctggaaga ccttgtgtgc atccgccccg gcctcgatga gatcggcggc
      901 gaggcgcagc gccttcggcg tcgtgttcgc gtactggaag cgtccggtgt cggtgacgac
      961 ggccgtgtag aggggctcgg cgagctccgg cgtgagcgca acgccgagct cttccatcac
     1021 atcggcgagc acctcgcccg tcgaagaagc ggcgggaacg accagatcga cgtcaccgaa
     1081 gagggtgttg tcgtggtggt ggtcgacgtt gatcgtgaac ggaaccgcct cgagcagcgc
     1141 ggccgactcg gggccgaggc ggctttcctg ggcacagtcg accgccagca gcgtccgctc
     1201 gccgagatcg tcgggcagct cgcgtctcac atcgtcgagc tggaggaagc ggtattcgcc
     1261 gggcagctga gactgaccgg ataggaacat cacgctgtcc tttcccagcc ggccgaggac
     1321 gaggtgcgca gcgagcagcg agccgagggc gtcgccgtcg gggctctcgt gtgtcgcgac
     1381 gacgaatcga tcgcgcgcgc ggagggcggc gacgaccgcc cccatctcat tctcgtgggt
     1441 catccggcgc ttctccaggg gccaactcgt cgatcagctt ggtgatccga acgcctcgct
     1501 cgaccgacgg atcgtacgcg aagaccaatt gcggagtccg tcgcagatgg agctgccggt
     1561 tcagctcgga ctggagcacg ccggcggctc cttcgagtgc cctcaaggtg gccgttcgct
     1621 cctgctccga gccgagcacg ctcaaccaga ccgtcgcctg cttgatgtcc gtggtcgtct
     1681 tcacgccggt gacggtgacg aaaccgatcc gggggtcctt gagggtcggg atcgcctccg
     1741 acagcacctc gcgaatcgcc tcatcgaccc tgcgcatgcg ctcactcgtc aagggtcacc
     1801 acccctcgcc tcgcctgcat caactcgaag tgctggccgg agagccaacg ctccgcctcg
     1861 tcgagcaggc gctcggtctc ccccgcctca cgcgccacgc aggcaagcgt gagccctgcc
     1921 cgctgccata cgtcgtggtg ctcgacctct gcgatcgagc agcccacgcg gttcgagagg
     1981 cgatccttca ccgagcgcac gaccatgcgt ttgtccttga gcgaacggct ctcgggaatg
     2041 aacaactcga acgcgtagat cccgacgtag ccggtggcca tcggggtgct cgatccgccg
     2101 gctactcgcc gggggtggcc gcggcggcgg gctcgtcgag ctcggtccgc tcgaccgcgc
     2161 gccgctcgta tgcctccagc acgtcgcctt ccttcacgtc gttgaagccg tcgagcagga
     2221 ttccgcactc gaagccctcg ctcacctcgc gaacgtcgtc cttgaagcgc ttgagttgcg
     2281 tgatcgccgt gtcctgcacg accgtgccgt cgcggacgac gcgtacgagc gcgcccctcc
     2341 ggatcgcgcc gtcggtcacc atgcagccgg cgatcgtccc aaggcgggag accttgaaca
     2401 gcgccctcac ctctgcctcg ccgagcgtct cctccgtctc gacgggcgag agcatcccga
     2461 ccagcgcctt ctggatgtcc tcggtgagct ggtagatgac ccggtaggtg cgaatgtcga
     2521 cgccgacacg ctcggcgagg ccgcgcgctt ccgcgctcgg tcgtacgttg aagcccacga
     2581 cgagcgcatt cgaggccgag gcgagattca cgtcgttctc ggtgattccg ccgaccccgg
     2641 tgtgaatgac gttcacgcgt acctccgagt gctgaatctt gccgagctcg cccacgagtg
     2701 cctcgatcga tccctggacg tctcccttga gcacgatgtt cagcacctgc acagcgccct
     2761 cttgcagttg gtcgaagagc gtttccagcg agacgcctcg agcggagcgc tgcgcgagct
     2821 gctctcggcg cagccgctcg gcgcgcaggt tcgcgaggtg cttggcctgc cgctcgtgct
     2881 cgacgacgcg ggccagctcg cccgcaggcg gcggcttgtc gaagccgaga atctcgaccg
     2941 ggtcgcccgg gccggcctcc gtcaccttct cgccgaagta gtcgtgcagc gcccgcactt
     3001 tcccagccgc gtcgcctgcg acgatcgcgt cgccgacctt cagcgtcccc cgctggacga
     3061 gcatcgtcgc gacgggcccc cggcccacgt cgaggcgcga ctcgatgatc gggcctgacg
     3121 cttccgcgtt cgggttcgcg cgcagatcga gctcggcgtc ggcgacgagc agaatgcgct
     3181 cgagcagatc gtcgaggttc tgctgctctt tcgccgagac ctcggagaac tgcgtcgttc
     3241 caccccagtc ctccggctgc agcccttcgt tggccaactc gcccttcacg cgatcggtat
     3301 tcgcgtccgc gacgtcgatc ttgttcacgg cgacgacgat cggcacctcg gccgcacgcg
     3361 cgtggctgat cgactcccgc gtctgcggca tgactccgtc gtcggccgcc acgaccagca
     3421 ctgcgatgtc ggtcactttc gcgccgcgcg cacgcatggc ggtgaacgcc tcgtgcccgg
     3481 gggtgtcgag gaaggtgatt cgccggtcgt ccacctcggc ctggtaggcg ccgatgtgct
     3541 gggtgatccc tccggcctcg gtctccacca cggcggtctg gcggatggcg tcgagcaacg
     3601 tcgtcttacc gtggtcgaca tggcccatga tcgtgacgac cggcggcctc ggctcgagat
     3661 ccgattcggc gtcctccacg gcaatcggct cgagatcttc ctcggatgcg tgcttgatct
     3721 cgacctcgcg gctcacctcg gcggcgatca gctcgacctc ctcgtcggag agggactgcg
     3781 tcgccgtgcg catctgcccg agccccatca gggtcttgat gatctgcggc atcgggacgc
     3841 ccagggcctg cgagaggtcc ttgacggtga cgcctgactc gacgatgacc ggccccgtcg
     3901 gctgctgcgg ttgctggggc tgcttcggct gcgccggcac gccacgatcg cgtggctgcc
     3961 gcgcttcccg cccgcgcgcg gcaccgccct cgatgacgac ccgacgcttg cggccggagg
     4021 agccgccgcg ggggcgaatc ggcctattcg gtctgttcga tccagccatg tcgtgtcaag
     4081 tgtagagacg cgcgagcgtg gactcgacct tgacgcttcg tcgcagcacc cggttgaacg
     4141 cccgtgcgga cgtcgctcgc tcgaaacagg agcggcggcg gcacgtgtac gcgccccgtc
     4201 ccggcagcgt ccgacccggt gtgagcaccc cgtccaccgc cgcgaagcgg atcagctcag
     4261 cctgcggtgc tctgcgcccg cagccgacac acgtccgttc cggctcgggg ctcacccacc
     4321 cgtcgcctcg ccgctcggag cctcttccgt cgcctcctcg gccaccggct gcccagggtc
     4381 ggccgcgtct tccacgatct cctccgcctc ggcgaccacc tcggcctcga gctcctcggt
     4441 gacgagtccc gaagctgcat cctcgaccat cacctcgacc gccgtcgtca gctcgacggg
     4501 cgactcctgg ccgaccagcg cctggtgagc cggaaccccg cagtacttcg aggccggcag
     4561 cgcagcattc ggacaccgtt tgccgttcga caggatcgca gcgcaacggc cggagaactc
     4621 ctcggtgtcg tcgtcgcccg cgaacgccgc atcggcctcc gcctgtgcga actcggtatc
     4681 ggactgaata tcgatcttcc acccggtgag gcgtgcggcg agtcgcgcgt tcagcccttc
     4741 cttgccgatc gcgagcgcca gctggtcgtc cggcacgacc acggtcgcgt cctttgtctc
     4801 gtcgtcgatg tagacctcgc gtacgcgagc gggcgacagc gccttcgcga cgaagcgggc
     4861 gggctccgtg ttccacggaa tgatgtcgat cttctcgccg cggagctccg agacgaccat
     4921 gcggacgcgc gagccgcggg ggcccacaca ggcaccgacg ggatcgacgc cctggacatg
     4981 cgactccacg gcgatcttcg ccctgtagcc gggctcgcgt gccacgccgc ggatctcgac
     5041 caggccgtcc gcgatctccg gcacctcgag ctcgaacagc gtcttgatca gctctgggtc
     5101 gcgtcgggag aggatgacct gaggtccttt cgtaccggag cgcacctcga tgatcaccgc
     5161 cttgatccgg ccaccctgct cgacccgctc cccgtcgacc tgctcggagc gcggcagcag
     5221 cgcctcgacg ttgttgccga ggtcgacgag aacgttgttg cggtccccgg cctgctgcac
     5281 gattccggtc acgacgtcgc cgacgcggtc gatgtactcc gcgtagcgga tcgcacgctc
     5341 ggcctcgcgg atgcgctgct ggatgacctg tttcgcggtc atcgccgcga tccgcccgaa
     5401 gtcgcccggc gtgacgtccg ttcgaaccat ctgggcgtcc ggcacctgcg accagtcgag
     5461 gtcgagatcg tcgtccgaga tcagggtgtg ttgacgttcc ccggtctctt cctcgacccg
     5521 ttccagctcg gcgatggcct cctcgcgcgc ctcgtcgagc agtcgctcct cgaggtcggc
     5581 ggggatgtcg atggcgaaaa cgcggaactc gccctcgtcg tcgagtatca cctcggcgtg
     5641 gcgcgctgct ccgggtgtct tcttgtaggc ggccagaagt gcatcctcga gcgcgtgcac
     5701 cagcgtgtcc tgctcgatgc ccttctcgct ctcgagcgca cggatgttct cgatgatctc
     5761 gatgctcatg actcagtcct atccgtcgtc gatcaggttg ccccggacga tcgcctcgta
     5821 cgggatgtca agctctccct ctgccgtccg aatcgtggcg tggtcgcgcc cggcggcggc
     5881 gatgactcct cggaatcgcc tcgtgccgcc gatctcgttc tcggtgcgaa tggccacgct
     5941 gcggcccagc gctcgttcga aatgcgccgc cgttcgaacc ggccgctccg ggcccggcga
     6001 ggacacgtcg atcgtgtagt cgacgcggta cccgtcgagc agccgcgtca cgcgctcgca
     6061 gagcgcgtgg tcgacccctt cagggtggtc gacgtagacg caaaaacgcg tcggcgacag
     6121 gagctccacg gcgagaacct cgacatccgg cagccgtcct tgcacggccg gggtgatgtc
     6181 ccgctcgagt tcacgctctg tcgcgtatgc gttggccaaa actgctgctc cttcacaaaa
     6241 aatgggcgcg acgcgcccat ttctccaacc ggtgaaatgt ggtcttcgga ggatagcagg
     6301 cggcttccga aaccgttctc gcgcgccgag cgcgccgtct agagcgaggc gtagagatcg
     6361 acggggacgc cgtccggatc gcggagctgc gcataccgct gtccccagaa cgcgtcccac
     6421 gggctcttgt ctcccgtgaa gcctgcgtcc gtgacacgtg catagatctc gtcgacgtcg
     6481 gcggggcgtg cacactcgaa ggcgagagcc agttgattcc cgctctcccg cacccattcc
     6541 ggccggaagc tccggatgac ctcctcggtg tcgagcatca agcggcagcc gttcggaaac
     6601 accgtctcga cgtgacctac gccgggggtc tccggcatgt ccagtccgag cagtcggtag
     6661 aagcgaatcg tctccgccat gtcggagacg acgacgccga cggcactcag ttgcggcacg
     6721 gcgacgacac taacggctaa gagcaatccc ggcggtcgcc gaggcgtgtc gggcgcgatg
     6781 cggggctcgg tgcattatcg cgacggcctc agctgagcgc gctcttgatc gcggatccct
     6841 ggcggttcaa gatgcacctg acacaagggc aagacggcat cggttgggcg acagcagacg
     6901 aaccgctctt gcagtctcaa aacgctcgaa tcgggaggca acggaggact ctgatgaagc
     6961 gcaagctctt gttgtcgatc gggcgcaagc cgcgaaagac ggccgacgag cttctgcgag
     7021 aagcgcgcac ccgcctccgg aggatgaccc ctcgagaggc tcttgccgca gtcagtaggg
     7081 gtgcgctgct catcgaccta cgttccgggg acgatcgccg ccgcgacgga atcattccag
     7141 actcacttca catcccgaga acggtgctcg aatggcgtgc cgaccccgcg tccgactacg
     7201 ccgacccgga gattggtgac gacctcggcc ggaccctcat tctcgtctgt acggacggct
     7261 attcctcgag cctcgcggcc gccacgttgc aggacctggg gttcgcggct gctacggacg
     7321 tgattggcgg ctatcgagcg tgggaagaag cagggctgcc gacgcgactg agcgccgaga
     7381 tgacgcgagt catctagccg ccctcgagat tggccgggag gacttcagcc ggccgcgccc
     7441 tcaggcggcg gctgcatggc ggcgccgggc agcggccgcg gctgtggctg gagagccggc
     7501 ttgggctctc gctccggctc ctcttcgggc tcctcgaccg gcttggcctc ctcggcgaag
     7561 acggattcct ccgcctcgcc ggcgagcaga cgctcgaatt ggtccttgtc gatcgtctct
     7621 cgctcgatga ggatcccaga gagcatgtga agctcctcga tgcgctcgcg cagcacgttc
     7681 gcggccatgt cgtgcgcctc ttcgatgaca cgacggatct cgtcgtcgat ctccttcgca
     7741 acctcgtccg agtagtcggg ctccgccgac atctcacggc ccaggaaggg ctggttgctg
     7801 tctcggccga gcacccgcgg gccgagtttc tcgctcatcc cgaagcgcat gatcatc
//
LOCUS       DBNGNK010000216         5130 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_111665,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000216 DBNGNK010000000
VERSION     DBNGNK010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5130)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5130)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5130
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_111665"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC150V_06405"
     CDS             <1..672
                     /locus_tag="AC150V_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012774019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HIR3762589.1"
                     /translation="DTLEKIQQDFNGSQSGGKKVSLADVIVLGGCAAVEEAAKRAGHD
                     VDVPFTPGRTDAGQDQTDVDSFAALEPTADGFRNYLQNGHDLEAEHLLVDKAFMLTLS
                     APEMTALVGGLRVLGANADQSRHGVFTDRPETLTNDFFVNLLDMGTEWKATSDAADVF
                     EGRDRESGDVRWTGTRVDLVFGSNSELRALAEVYGCGDAKEKFVDDFVTAWDKVMSLD
                     RFDVG"
     gene            complement(912..2441)
                     /locus_tag="AC150V_06410"
     CDS             complement(912..2441)
                     /locus_tag="AC150V_06410"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3762590.1"
                     /translation="MPIVKVMLSRLIHQAVSPRAMMLLPFAFMTALAAALSFPISHVG
                     EAPWKEWVAVGLTGLLIVAGFALPWHTMPRYTHPIVPLAYFAVIFLLRDANGGAGSGF
                     EALCVMPVFWLALFGTRRQLALALLAVAIVFVAPVLFIGGERYPGTEWRSAALWTALL
                     TIVGYRVHALITEIKRRAGIARGSAESAEQTAQELAETQHALKTMADLARAVPTAPDA
                     RELICQAAVAGSGATLATLVEPDAEGGFRVTGSAGVPLDPKRLRADVRPSASVRAFYA
                     RRRIFVADARLDPGVSKAIMDATGLRSIVYEPIVRHAKPVGVLCVGWDEQLPELDIPS
                     ATVISYLAAEAGAAIERADTVAQLDDLASTDQLTGLPNRRSWDIHLARAAGGSSERPF
                     CMAIIDLDHFKRYNDLHGHQAGDTLLRAAAIAWQQQLRTDDVLARYGGEEFAVLLPGC
                     PVDQAQRVLDRLRRSTPTVTCSIGLAEHEAGETTEHLTARADQALYTAKRRGRDQLIA
                     A"
     gene            complement(2729..4222)
                     /locus_tag="AC150V_06415"
     CDS             complement(2729..4222)
                     /locus_tag="AC150V_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3762591.1"
                     /translation="MTPPEQSYPRREFLARAGGLAGASGALLLSGRGAHAVIVQSPAS
                     DPIADRAVQAARQFKGAKLGFAPVAGKERPDEEASFAAKWTDLTGVAVELDPEPKDVF
                     EAWTAPPFGSLPDFAENGWIVPLDELIAKHRASASIRDYHPLNRSFAVYRGKTWGFVD
                     DSNPFFLCYRTDIFADADLRRSYRALFNRELRVPRTWGEFAETAQFITDQRAPAVYGT
                     GMGREFAVGAGGDLYFQFHFHLNGGRFFDLETMRARINGPAGAKAVQQLLAENAASVP
                     GIEKLNFTHTMGLWLQGRTAMLMHWGASGRFAENAHRRAGLWKALPRSKIAGKVAYAV
                     VGRHALDGGGTLQMVSSGSKHTEAAYLYVQWLTSPSIQYERLKVPITDRDPSRTSHFR
                     QPGLFGDWLTVDEFARTHCEAANNAVLPLFIAGGSDYAQALHVGLQAIYRHKRIQEGL
                     DDVAAQWDAITKKRGVDNQRRSYRSYLKQTGSSREMTAAARGLAVEC"
     gene            complement(4219..>5130)
                     /locus_tag="AC150V_06420"
     CDS             complement(4219..>5130)
                     /locus_tag="AC150V_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3762592.1"
                     /translation="LEITTDSIQHVDPETNELVATIEAPGFLMIESGGGSVWAANADE
                     NRIWRIDPRTHRVVQTIEGIVNPELFAFGAGALWALNPIDGIVTRIDPESGDVTHVVP
                     IPRGAPGGVLGPSGMRAGEHGVWLAWTSPEGNAVHIDPRSLAARVVQVGSVDFSQTID
                     FAQVDESTLWVLAPNSAKLGAVSLLKVDPSTPGRVEADVPGFDWGLLAMDGESIWSSR
                     QFENSVSRFETSTRQLQGLVSVGAFPSGVAVGAGALWVGNWVDGTLSRVDLDSQKAGE
                     PIAVGPNPRSLAVTEDGVWVAVGPEDS"
BASE COUNT          824 a         1757 c         1739 g          810 t
ORIGIN      
        1 gacaccctcg agaagatcca gcaggacttc aacggctcgc agtcgggcgg gaagaaggtc
       61 tcgctcgccg acgtgatcgt gctgggcggc tgcgcggccg tcgaggaggc ggcgaagagg
      121 gcgggacacg acgtggacgt tccgttcacg cccgggcgca ccgacgccgg tcaggaccag
      181 actgacgtcg actccttcgc cgcgctggag cccacggcgg acgggttccg caactacctc
      241 cagaacggcc acgatctgga ggcggagcac ctgctcgtcg acaaggcatt catgctgacg
      301 ctgtccgcgc ccgagatgac ggcgcttgtc ggtggcctgc gcgtccttgg cgccaatgcc
      361 gaccagtccc ggcacggcgt cttcaccgac cggcccgaga cgctgaccaa cgacttcttc
      421 gtcaacctgc tcgacatggg cacggagtgg aaggcgacct ccgacgccgc ggacgtgttc
      481 gagggtcgcg atcgcgagag cggcgacgtc cgctggaccg gcacccgcgt cgacctggtc
      541 ttcgggtcga actccgagct ccgtgcgctg gccgaggtct acggctgcgg cgacgcgaag
      601 gagaagttcg tcgacgactt cgtgacggcg tgggacaagg tgatgagcct cgaccgcttc
      661 gacgtcggct gaccgggacg acctgaccgc gacgaaccgg cccgtttcga gcacgcggcg
      721 ggttcgcgcg gtctggtccc gatgcgggac tccgtccggg ggccgaatcg ctcaagggcc
      781 atcgacggtt actccgcgac gtcgccgcct gatccaaccc gcgcgcgcgg gctcgcgtgc
      841 gcttgtgaca cggtctcacc ctgcggccag gtcgaccggc agcggggctc ggtgaccggc
      901 tctcgcggcg gtcaggccgc gatcagctga tcgcggccgc gccgcttggc cgtgtacagc
      961 gcctggtctg cgcgcgcggt gaggtgctct gtcgtctcgc cagcttcgtg ctcggcgagg
     1021 ccgatcgagc acgtgaccgt cggcgtcgac cgccgcagac gatcgaggac tcgctgggcc
     1081 tgatccacag ggcacccggg cagcaggact gcgaactcct cgccgccgta gcgggcgagc
     1141 acgtcgtcgg tgcggagctg ctgttgccag gcgatggcgg ccgcgcggag gagcgtgtcg
     1201 ccggcttggt gaccgtgcag gtcgttgtac cgcttgaagt ggtcgaggtc gatgatcgcc
     1261 atacagaacg ggcgctcgct cgagccgccg gcggctcgcg ccagatggat gtcccagctg
     1321 cggcggttcg gcagccccgt gagctggtcg gtgctcgcca gatcgtcgag ctgcgcgacc
     1381 gtgtcggcac gctcgatcgc cgccccggcc tccgcggcga ggtacgagat cacggtggcg
     1441 ctcgggatat cgagctcggg aagctgctcg tcccagccga cgcagagcac gccgaccggc
     1501 ttcgcgtgac gcacgatcgg ctcgtagacg atcgaccgta gcccggttgc gtccatgatc
     1561 gccttggaca caccggggtc gagccgtgcg tccgccacaa agatgcggcg ccgcgcgtag
     1621 aaggcacgga cgctcgcact cggccggacg tcggcgcgga gccgcttagg atcgagcggg
     1681 acgccggccg agccggtgac gcgaaagccc ccctcggcgt ccggctcgac cagcgtcgcg
     1741 agggtcgcgc ccgagcccgc gactgcggcc tggcagatca gctctcgcgc gtccggcgcg
     1801 gtggggacgg cgcgcgccag gtcggccatc gtcttgaggg cgtgctgcgt ctcggcgagc
     1861 tcctgcgcgg tctgctcggc ggactcggcg cttccccggg cgattccggc gcgccgcttg
     1921 atctcggtga tcagcgcgtg cacccgatag ccgacgatcg tcagcagcgc cgtccagagc
     1981 gccgcgctac gccactccgt gcccggatag cgctccccgc cgatgaagag gacgggggcc
     2041 acgaagacga tcgcgaccgc cagcagcgcg agcgcgagct ggcgtctcgt gccgaacaga
     2101 gcgagccaga acaccggcat cacgcagagc gcttcgaagc cggatccggc accgccgttc
     2161 gcgtcgcgga gcaggaaaat cacggcgaag taggcgagcg ggacaatcgg atgcgtgtag
     2221 cgcggcatcg tgtgccaggg cagagcaaaa cccgcgacta tcagcagacc cgtgagcccg
     2281 accgcgaccc actccttcca cggggcttcg ccgacgtgcg agatcgggaa cgagagcgcg
     2341 gcagcgagcg ccgtcatgaa ggcgaacggc agcagcatca tcgcgcgcgg cgagaccgcc
     2401 tgatggataa ggcgcgagag catcaccttc actatcggca ccgatcgggc cttcaagaac
     2461 gcgagatccg ccgtccgaat ccccgagaag cggcttccct cccccgggac gggccttttc
     2521 tgagtgggcg gtgacgggct cgaaccgccg acctcctcca tgtaaaacac gtgaccaagg
     2581 gagcgccgga accgcatgaa caagcggttt ccgcgctcct atcggactct tgaaggccag
     2641 ttcagatccc tgtgcttgcg ggctttcggg aggattcggg cactgaaatc gagtttgtgc
     2701 ccaaacctcc gccgaacatg ccctcgtatc agcactcgac cgcgaggccg cgggcggccg
     2761 cggtcatctc gcggctcgag ccggtctgct tcaggtagct ccggtacgag cggcgttgat
     2821 tgtcgacgcc gcgtttcttc gtgatcgcgt cccactgcgc ggccacgtcg tccaggccct
     2881 cctggatgcg cttgtgccgg tagatcgcct ggagcccgac gtggagtgcc tgggcatagt
     2941 cggagccgcc ggcgatgaac agcggcagca ccgcgttgtt cgcagcctcg cagtgcgtcc
     3001 gggcgaactc atccacggtg agccaatcgc cgaagagccc cggctgccgg aagtgagagg
     3061 tccgcgacgg gtcgcggtct gtgatgggca ccttcagccg ctcgtactgg atggacggac
     3121 tggtcagcca ctggacgtag agataggcgg cctccgtgtg cttcgagccg ctgctgacca
     3181 tctggagcgt gcctccaccg tcgagcgcat gacgtccaac caccgcgtag gcgaccttgc
     3241 ccgcgatctt cgatctgggc agcgccttcc acagtcccgc ccttcggtgc gcgttctccg
     3301 cgaaccgacc cgaagcgccc caatgcatga gcattgccgt tcggccctgg agccagagcc
     3361 ccattgtgtg ggtgaagttg agcttctcga tgcccggtac cgaggcggca ttctcggcga
     3421 ggagctgctg cacagctttc gctccggcgg ggccgttgat cctcgcgcgc atcgtttcga
     3481 gatcgaagaa gcgaccgccg ttcaggtgga agtggaattg gaagtacaaa tcgccgccgg
     3541 cgcccacggc aaactcccgc cccattcccg tcccgtagac ggccggcgcc cgctggtcgg
     3601 tgatgaactg cgccgtctcg gcgaactcgc cccacgtacg cggcacccgc agttcgcgat
     3661 tgaagagagc ccgatacgag cggcggaggt ctgcgtccgc gaagatgtcg gttcggtagc
     3721 agagaaagaa cgggttgctg tcgtcgacga agccccaggt cttcccgcgg tagacggcga
     3781 acgatcggtt cagcgggtgg tagtcgcgga tgctcgccga tgctcgatgc ttggcgatca
     3841 gctcgtcgag cgggacgatc cagccgttct ctgcaaagtc cgggaggctc ccgaaggggg
     3901 gagcagtcca cgcctcgaac acgtccttcg gttcggggtc gagctcgacg gccacccctg
     3961 tcagatccgt ccactttgcg gcgaagctcg cttcttcgtc cggccgctcc ttcccggcga
     4021 caggtgcgaa tccgagcttc gcccccttga actgcctcgc ggcctgcacg gccctgtcgg
     4081 cgatgggatc cgacgcgggc gactgcacga tcaccgcgtg ggcgcctcgg cccgaaagga
     4141 ggagtgcgcc ggaggcgccc gcaagcccac ccgcccgtgc gaggaactcc cgccgtggat
     4201 agctctgctc tggcggcgtc atgaatcttc cgggcctacg gcgacccaga ctccgtcttc
     4261 ggtgacggca agcgacctcg ggttcgggcc gacagcgatt ggctctccgg ccttctgcga
     4321 gtcgaggtcg acccgcgaga gcgttccgtc gacccagttc ccgacccaca acgcgccggc
     4381 gcccacagcg accccggagg gaaacgcccc cacggacacg agtccctgca gttgacgcgt
     4441 gctcgtctcg aagcgggaga cgctgttctc gaattgcctg ctcgaccaga tgctctctcc
     4501 gtccatcgcc agcaaccccc aatcgaatcc gggcacgtcg gcctcgaccc tccccggcgt
     4561 gctcgggtcg accttgagca acgagactgc accgagcttg gccgagtttg gagcgagcac
     4621 ccagagcgtc gactcgtcga cctgcgcaaa gtcgatcgtt tgcgagaagt ccacggaccc
     4681 gacctgcacc acgcgagccg caaggctccg cggatcgatg tggacggcat tcccttccgg
     4741 gctcgtccac gcaagccaga caccgtgctc gccggcccgc atgccggaag gcccgagcac
     4801 gccacccgga gcgccccgag gaatgggcac tacgtgtgtg acgtcgcccg actcgggatc
     4861 gatgcgcgtg acgatgccgt cgatcgggtt gagcgcccag agagcgccgg ccccgaacgc
     4921 gaacagctcg gggttgacga tgccctcgat cgtctgcacg actcgatgtg tcctcgggtc
     4981 gatccgccag atccggttct cgtcggcgtt cgccgcccaa acggagcctc caccggactc
     5041 gatcatcaag aacccgggcg cctcgatcgt ggcgaccagc tcgttcgtct ccgggtcgac
     5101 gtgttggatc gagtcggttg tgatctcgag
//
LOCUS       DBNGNK010000217         3561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1831744,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000217 DBNGNK010000000
VERSION     DBNGNK010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1831744"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="AC150V_06425"
     CDS             complement(<1..1607)
                     /locus_tag="AC150V_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HIR3762593.1"
                     /translation="MRRSLGAWGRLYLDRPLPFLCVYRRPARRPDPGTLELITGEASY
                     LVASGLARHQADVVGTVQAIAERRLEEFGGFLIVEVWSGPGAHAAADGAEEPAFSIVK
                     RRGSAITDTADELEAALGRIRAPALPRLAVTMDERQVVRPPGMRPLITDETIEDGGWH
                     VLGLRVPDIYVNDATGELYPLVHRVLRRSLSGALRRAFFQFTKRHTDHRPPHYQALGA
                     RSLTKSVWSVDRQLANIAGQFDFLLLTTPRNIEEAWHEFRRGRFERPPRFSYRPSPVD
                     LSLAKRDLYNIRVERVEDPILSELFREQQDELDRKLTMIGDRQTRRFRQESQQLFGGV
                     ETELLQDALEILEGIPSRARERSGGDPVDARSFARKAEREIAALKKLEDRVRARVEIR
                     SDVNGLMVSHGNVLVGSTLSVPPARVEALIQHEVGTHVLTHVNGAAQPFRQLRTGLAS
                     YEELQEGLAVMAELLVGGLSRPRLRVLAARVAATSQMLDGATFVDVFRRLDGGWGLSQ
                     RIAFTVTVRVFRGGGHTKDTVYLRGLLR"
     gene            complement(1703..2563)
                     /locus_tag="AC150V_06430"
     CDS             complement(1703..2563)
                     /locus_tag="AC150V_06430"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015191073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050129 - N-formylglutamate deformylase
                     activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR3762594.1"
                     /translation="MKEKPSLAEAAGGDPFPDSEVLWDVVEGDDCIVAIALHHGHVVR
                     EEVSASFAIEAAERRREEDPHTGSWTEIAATRLVKHRSRFEFDVNRPRERAVYLRPED
                     AWGLRVYDPPLPPEVVARSLAYYDAFYTEAQAMIERLLESHEAIVILDLHSYNHRRGG
                     PDAEPADAAENPEVNVGTASIDRDRFGPLIDRFIADLGFHEFLGRRLDVRENVRFFGG
                     NFSRWTHASFPGRVCTLAVEFKKFFMDEWTGELHDDVWSEIPRALDATLPGLRAELEA
                     LSDSRRGGAA"
     gene            2827..3444
                     /locus_tag="AC150V_06435"
     CDS             2827..3444
                     /locus_tag="AC150V_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762595.1"
                     /translation="MPAALFLALAGCGGSSDSSSGQNDTPETTTETTTDTGTTEADTE
                     TTEAEPETTEPDTDLTETETAETETTETETTAPSTNSGTVPPPGEATAEIDGKTFVFL
                     ASDTLSERLLACEISNDMVTVNFQTDGHDILIQVREVESEWLGNITVAPREVEGSYGS
                     DVRSSGEFVVDGQSAVYTGGFTFSPFENRADFQDVGEGRIAVTCP"
BASE COUNT          614 a         1220 c         1186 g          541 t
ORIGIN      
        1 cgcagaaggc ctctcaagta caccgtgtcc ttggtgtgac cgccgccacg gaagacgcga
       61 accgtaacgg taaacgcaat ccgctgcgac aatccccaac cgccgtcgag ccgcctgaaa
      121 acgtcgacga acgtggctcc atccagcatc tggctcgttg cggcaacccg ggctgcgagc
      181 acccggaggc gcggccggct caggccgccg acgaggagct cggccatgac ggcaagcccc
      241 tcctggagct cctcgtacga cgcaaggcct gttcgtagct gtcggaaagg ctgggcggcg
      301 ccgttcacgt gcgtcaggac gtgtgtgccg acttcgtgtt ggatgagcgc ctccacccgc
      361 gcgggtggga cgctcagcgt cgacccgacg agaacgttcc cgtgcgagac catgagcccg
      421 ttgacgtcgc ttcgtatctc gacgcgggcg cgcacacggt cttcgagctt cttcagcgcg
      481 gctatctccc gctcagcctt ccgcgcgaac gaccgtgcgt cgaccgggtc gccacccgaa
      541 cgctcgcgtg ctcgcgacgg gattccctcg aggatctcga gcgcgtcctg cagcagctcc
      601 gtctcgacac cgccgaacag ctgctggctc tcctggcgaa agcgtctcgt ctgccggtcg
      661 ccgatcatcg tcagcttgcg atcgagctcg tcctgctgct cgcggaagag ctccgacaga
      721 atcgggtctt cgacccgctc gacgcgaatg ttgtagaggt cgcgcttggc gagagagaga
      781 tcgacggggc tcggacggta agagaagcgg ggtgggcgct cgaaccgccc ccgccggaac
      841 tcgtgccatg cctcctcgat gttgcggggc gtcgtcagga gcaggaagtc gaactggccc
      901 gcgatgtttg cgagctgtcg atcgacgctc cacacgctct tcgtgaggct ccgcgctccc
      961 agagcctggt agtgaggagg tcggtggtcg gtgtggcgtt tcgtgaactg gaagaacgcc
     1021 cgccgcagcg cgcccgagag cgaccggcgg agtacccgat gaacgagcgg atacagctct
     1081 cctgtggcgt cgttgacgta gatgtccggc acccgcaggc caagcacgtg ccaaccgccg
     1141 tcctcgatcg tctcgtccgt gatgagcggc cgcatcccgg gcggtctgac gacttgccgc
     1201 tcgtccatcg tgaccgccaa gcgcgggagc gccggtgcgc ggatccggcc gagcgcggcc
     1261 tcgagctcgt ccgccgtgtc ggtgatcgcg ctcccccggc gcttgacgat cgagaaagcc
     1321 ggctcctcag cgccgtctgc cgcggcgtga gcgccagggc ccgaccagac ctcgacgatc
     1381 aggaagcccc cgaactcctc gagccgccgc tcggcgatcg cctgcacggt gccgacgacg
     1441 tcggcctgat gacgcgccag gcccgacgcc acgagatagg aggcctcgcc ggtgatcagc
     1501 tccagggtgc ctggatcggg gcggcgtgcc ggccgccggt agacgcacag gaacggcaac
     1561 ggacgatcga ggtagagacg accccacgcg ccgaggctac gccgcacccg gccgcccact
     1621 tccagccggg cacgcacgcc ggcgagctcg tcgcccggcg gctcggtcga gtccgttggg
     1681 cggctacccg gaccgtgttc gctcaagctg cgccgccacg acgagagtcc gacagcgctt
     1741 cgagctcggc tcgaagaccg ggcaaagtgg cgtcgagcgc gcgagggatc tcgctccaga
     1801 cgtcgtcatg gagctcgcct gtccactcgt ccatgaagaa cttcttgaac tccactgcca
     1861 gcgtgcagac ccggcccgga aagctcgcat gcgtccagcg cgagaagttc cccccgaaga
     1921 acctcacgtt ctcgcggacg tcgaggcgcc gcccgaggaa ttcgtggaag ccgagatccg
     1981 cgatgaagcg gtcgatcagc ggcccgaagc ggtcgcggtc gatcgacgcg gtaccgacgt
     2041 tcacctccgg attctccgcg gcatccgccg gttcggcatc ggggccgcct cgacgatgat
     2101 tgtagctgtg gaggtcgagg atcacgatcg cttcgtgtga ctcgagcagg cgctcgatca
     2161 tcgcttgcgc ctccgtgtag aaggcgtcgt agtaggcgag cgatcgcgct acgacctcgg
     2221 gcgggagagg tgggtcgtag acccggagac cccaggcgtc ttccggccgc aggtacacgg
     2281 cccgctcgcg cggccggttc acgtcgaact cgaagcgcga acggtgcttc acgaggcgcg
     2341 tcgcggcgat ctcggtccac gatcccgtgt gcgggtcctc ctcccggcgt ctctccgcag
     2401 cctcgatggc gaacgaagcc gagacctcct ctcgcacgac gtgcccgtgg tgcagcgcga
     2461 tcgcgacgat gcagtcgtcg ccttcgacga cgtcccagag aacctccgaa tcggggaaag
     2521 gatcgccgcc agcggcctcg gcgagcgagg gtttctcctt catctgcggc tcccgcggcg
     2581 tggggcaaag actgcgccca ccgatccgtc gagggccaaa gagccccgga ggtgacccgc
     2641 ggaggcgcgc atgcgcgcag agtactcatc accgcggggg cggcccgaag cgcgcactcg
     2701 agctggactc gaacggctcg gcttgattac atcgttcgta tccgagtgcc cgcgtagggg
     2761 tcttgccgcc gacacgatcg agaggtcacc atgcagcgaa atcgccgtct tctcctgctt
     2821 gccgccttgc ccgcagcgct ttttctcgct ctcgccggct gcggcggcag cagcgacagc
     2881 tcgtccggac agaacgacac cccggagacg acgaccgaga cgaccaccga tacgggcacg
     2941 acggaggccg acaccgagac gacggaagcc gagccggaga cgaccgagcc cgacaccgac
     3001 ctgactgaga cggagacggc tgaaaccgag acgaccgaga cggagacgac tgcgccgagc
     3061 acgaacagtg gaaccgtccc gccgcccggt gaggcgaccg ccgaaataga cgggaagacg
     3121 ttcgtctttc tggccagcga cacgctctcc gagcggcttc tcgcctgcga gatctcgaac
     3181 gacatggtga cggtcaactt ccagacggat ggccacgaca tccttatcca agtgagagaa
     3241 gtggagtccg agtggctcgg gaacatcacc gtggcgccca gagaagtcga gggaagctat
     3301 ggctcggacg tgagatcctc cggcgagttc gtcgtcgacg gacagagcgc ggtctatacg
     3361 ggaggcttca ccttctcgcc cttcgagaac cgcgccgatt tccaggacgt cggagaaggc
     3421 cgcatcgccg tgacctgccc ctagctcgcg cgcgcggaga agcgggcggc taccctcgaa
     3481 cgatggccag agcggccgtc gccgccgccc tcgcagcgct ggcgctgacc ctcaccacgt
     3541 gcggtggcaa cggcgagggt t
//
LOCUS       DBNGNK010000218         4246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_112252,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000218 DBNGNK010000000
VERSION     DBNGNK010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_112252"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1662
                     /gene="metG"
                     /locus_tag="AC150V_06440"
     CDS             139..1662
                     /gene="metG"
                     /locus_tag="AC150V_06440"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR3762596.1"
                     /translation="MTYYATTPIYYVNSTPHIGHAYTTIAADILVRHMRQRGEETFFL
                     TGVDEHAAKVARVAAEQGLSPQAYADEIVEVWRELPRRLDATNDFFIRTSDEEHKAFV
                     QDFLQRIYDNGDVYQDVYAGLYCVGCEAFKTEAELVDGKCPEHDREPEWIEERNWFFR
                     LSSYQEQLLALYDSRPEFVLPAFRYNEARSFIAGGLQDFSISRASQTWGIPIPWDQEQ
                     VAYVWADALVNYLSALTYARAGEDLRPQFWPGVRHLLGKDILRFHCVYWPAMLLAAGY
                     DVPHQLFVHGYLLLDDRKISKSLGNIVDPLDLIDVYGADAVRFWSARAVSFGQDGSAS
                     IEGLHDRYERELGNDLGNLLSRTTAMIVRFRDGRLRALPSSDSAVKEVVDGLGTAVAG
                     RLDSYDITTALEDAWEVVRALNRHVESTAPWQIAKDEARSEELDRVLYDLADGLRALA
                     VALAAYLPTTSSRILEALRQPADLDWKLVAYGQAVEAEGITPAEPLFPRIDAPTTAA"
     gene            1659..2405
                     /locus_tag="AC150V_06445"
     CDS             1659..2405
                     /locus_tag="AC150V_06445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR3762597.1"
                     /translation="MIDTHAHLDACDEPAFALVARARAAGVERIVTIGTGIASCLRAL
                     AIADEGEEVVAALGIDPHQASSPEADRVGELRDLLSHPKAVAVGEIGLDGYRGRSTLK
                     DQQALFDAQLELAAELNLPVVIHCREADEETAATLAPFPGTVILHCFSSPALLGVALE
                     RGYFVSFAGNVTYPKAADLRQAAAAVPADRILAETDSPYLTPQPVRGQRNEPAHVLHT
                     LAVLAEARGEDVSELEAAIDANAGTAFGLR"
     gene            2402..3178
                     /gene="rsmA"
                     /locus_tag="AC150V_06450"
     CDS             2402..3178
                     /gene="rsmA"
                     /locus_tag="AC150V_06450"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR3762598.1"
                     /translation="MNAAPKKALGQHFLADPNILGVAGRLAELGGDDVVLEVGPGLGA
                     LTVYLADRVQLVHAIEIDRSLEGPLTEAIGGRSNVRLRFGDALGLDLGSLEPHPTKLV
                     ANLPYNVATPLVMESIEGLPTVRLWCVMVQREIADRFFASPSTKAYGAVSVLLQLAAR
                     RTGFHPVSRTVFRPPPNVDSALVAFRRLDLPPNFSLIRRVVEGSFAHRRKTLANSLAL
                     SGVASREKAAAALELIGKQAAVRAEALAPGDFGALTKELA"
     gene            3292..3987
                     /locus_tag="AC150V_06455"
     CDS             3292..3987
                     /locus_tag="AC150V_06455"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR3762599.1"
                     /translation="MTLETAAELEVSGFDDDTLVREALLRLSQRVGCDPGWHVRIEKR
                     IPVAAGLGGGSSDAATALRLANDAVTAPLGGAQLHMLAAELGADVPFFLYEGPQLGTG
                     DGTELQPLAALPELVVLLILPAGEQKQSTADVYRRFDRRDGAVGFEERRRALVDRLHD
                     GLRAEDLPSLPRNDLVSSPLADRLEELGALRADVSGAGPVVYGLFADAAVATSAADAV
                     HEAGRIWATSLCC"
     gene            3999..>4246
                     /locus_tag="AC150V_06460"
     CDS             3999..>4246
                     /locus_tag="AC150V_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762600.1"
                     /translation="MTGEAFRGASARVPAVIEHRESRPGRILRRNRVLIAFAIAAVEG
                     VLVLTGTLPWWIVLALAAGALAVYLAAGRDSRVPEVRSV"
BASE COUNT          654 a         1450 c         1433 g          709 t
ORIGIN      
        1 acggaaacga gtccgaccgg gggcgggttg gggagctgct cgtgtccgcg agcctcgtgc
       61 tcgcaggagt cctggccgtg ctcctcaggg cacgacgcgg aagagacacg ccgcgtgcgc
      121 tccgtagaat cgggcggaat gacgtactac gcgaccacgc cgatctacta cgtgaactcg
      181 acgcctcaca tcgggcacgc gtacacgacg attgctgcgg acattctcgt ccggcacatg
      241 cgtcagcgcg gcgaggagac cttcttcttg acgggagtgg acgagcacgc tgcgaaagtc
      301 gcccgggtcg cggcggagca ggggctgtcg ccgcaggcat acgcggacga gatcgtcgag
      361 gtctggcgcg agcttccgcg ccgtctcgac gcgaccaacg acttcttcat ccgcaccagc
      421 gacgaggagc acaaggcctt cgttcaggac ttcttgcagc ggatctacga caacggcgac
      481 gtctaccagg acgtgtacgc gggtctctac tgcgtgggct gcgaggcgtt caagaccgag
      541 gccgagctcg tggacggcaa gtgccccgag cacgaccgtg agcccgagtg gatcgaggag
      601 cggaactggt tcttccgtct gtcttcgtac caggaacaac tccttgcgct gtacgactcc
      661 cgcccggagt tcgtcttgcc cgctttccgc tacaacgaag caagaagctt catcgccggc
      721 ggcctgcagg acttctcgat ctcgcgggcg agccagacgt gggggatccc cattccttgg
      781 gatcaggagc aggtcgccta cgtctgggcg gatgcactcg tcaactacct gagcgcactc
      841 acctatgcgc gcgccggaga ggatcttcga ccgcagttct ggccgggcgt gcgccacttg
      901 ctcgggaagg acattctccg cttccactgc gtctactggc cggcgatgct gcttgccgcc
      961 ggttacgacg tcccgcatca gctcttcgtg cacggctact tgttgctgga cgaccggaag
     1021 atctcgaagt cgctgggcaa catcgtcgac ccactcgacc tgatcgacgt ctacggcgcc
     1081 gatgcggtgc ggttctggtc agctcgcgcc gtgtcgttcg ggcaggacgg cagtgcgtcg
     1141 atcgagggac ttcacgatcg ctacgagcgt gagctgggga acgacctcgg taacctgctg
     1201 tcgcgaacga cggcaatgat cgtgcgcttt cgcgacggac gtctgcgggc gctgccctcc
     1261 tccgactcgg cagtgaaaga ggtggtcgac ggcctgggca ccgcggttgc ggggcggctc
     1321 gactcctacg acatcacgac tgcgctcgag gacgcctggg aggtggtgcg agcgctgaac
     1381 cgtcacgtcg agtcgacggc accgtggcag attgccaagg acgaggcgcg cagcgaagag
     1441 ctcgaccgag tgctctacga cctcgctgac ggcctgcggg cactcgcggt agctctcgcc
     1501 gcatatctcc cgacgacgtc gtcgcgcatt ctcgaagcgc ttcggcagcc cgccgatctc
     1561 gactggaagc tcgtggccta cggccaggcc gtcgaggccg agggaatcac gccggctgag
     1621 ccgctcttcc ctcggatcga tgcgccgacc acagcggcgt gatcgacacc cacgcgcacc
     1681 tcgatgcgtg cgacgagccc gcgttcgcgc tcgtcgcccg agcgcgcgcg gcgggggtcg
     1741 agcggatcgt caccatcggc acggggattg cgtcatgtct tcgtgcgctc gcgatcgccg
     1801 acgaaggaga ggaggtcgtc gcggctctgg gcatcgatcc gcaccaggcc tcgtcgccgg
     1861 aggccgatcg cgtcggcgag ctgcgcgacc tcctctcgca tcccaaagcg gtcgcggtcg
     1921 gcgagatcgg cctggacggc taccgcggac gctccacgtt gaaggatcaa caggccctct
     1981 tcgacgctca gctcgagctc gcggcagagc tgaacctgcc ggtcgtgatc cactgccggg
     2041 aagcggacga ggagacggct gccacgcttg ctcccttccc gggaaccgtg atcctgcact
     2101 gcttctcttc tcctgcgctg ctcggcgtcg cgctcgagcg agggtacttc gtgtcgttcg
     2161 cagggaacgt cacctacccg aaggcggcag atctgcgcca ggctgcggcg gctgtgcccg
     2221 ccgaccggat cttggcggag accgacagtc cgtatctgac gccccagccc gtccgtggcc
     2281 agcgcaacga gccggctcac gtgctgcaca ccctcgccgt gctcgcggag gcgcgggggg
     2341 aggacgtctc cgagctcgag gcggcgatcg acgcaaacgc gggcaccgcc ttcggcctgc
     2401 ggtgaacgct gcaccgaaga aggcgctcgg gcagcacttc ctcgcggatc cgaacattct
     2461 cggggtggcc gggcggctcg cggagctcgg tggcgacgat gtcgtgctcg aggtggggcc
     2521 cgggctcgga gccctgacgg tctacctcgc cgaccgagtc cagctcgtcc acgcgatcga
     2581 gatcgatcgg tcattggagg ggcccctcac cgaggccatc ggaggacggt cgaacgtcag
     2641 gctccggttc ggcgacgcgc tcggcctcga cctaggctca ctcgagcccc acccgacgaa
     2701 gctcgtcgcc aacctcccgt acaacgtggc gacaccgctc gtgatggaga gcatcgaggg
     2761 cctgccgacc gttcggctct ggtgcgtgat ggtgcagcgg gagatcgccg atcgattctt
     2821 cgcctctccg tcgacgaagg cgtacggtgc cgtctcggtt ctgctccagc tcgcggctcg
     2881 ccgaaccggc ttccacccgg tctcccgcac cgtctttcga ccgccgccca acgtcgactc
     2941 cgcgctggtc gcgtttcgtc ggctggatct tccgccgaac ttctcgctga tccgacgcgt
     3001 cgtcgaagga tcgttcgcgc accggcgcaa gacgcttgcg aattccctgg cgctgtccgg
     3061 cgtagcttct cgcgagaaag cagccgccgc actcgagctc atcggaaagc aagcagcagt
     3121 ccgcgccgaa gccctggctc caggcgactt cggggcgctg acgaaggagc tcgcctgagc
     3181 cgcgcggctg cccacgccaa gatcaacctc gcacacgtcg tcgggccgct gcgccccgac
     3241 gccaagcacg aggtcgcaac gatcctgcag cggctggagc tctgtgacga ggtgacactc
     3301 gagacggcgg ccgagctcga agtcagcggc ttcgacgacg acacccttgt ccgcgaggcg
     3361 ctgctccggc tgtcccagag ggtcgggtgt gatcccggct ggcacgtgcg gatcgagaag
     3421 cgcattccgg ttgcggccgg gctcggcggt ggaagttcag acgccgcaac ggcgctccgg
     3481 ctggccaacg acgctgtcac ggcaccgctc ggcggcgccc agctccacat gctcgcagcc
     3541 gagctcggtg ccgatgttcc gttcttcctg tacgaggggc cgcagctcgg taccggcgac
     3601 ggcaccgagc tacagccgtt ggccgcgcta ccggagctcg tcgtgctcct catcctgccg
     3661 gccggcgagc agaaacagtc cacagcggac gtctaccggc gcttcgatcg gagagacggc
     3721 gcggtcggct tcgaggagcg gcgccgcgct ctcgtcgacc ggctacatga tgggctccgc
     3781 gcagaagacc tcccgagctt gcctcggaac gacctcgttt cgtcgccgct cgccgaccga
     3841 ctcgaagagc tcggtgcgct tcgcgccgac gtgagcggcg ccgggccggt ggtctacggc
     3901 ctcttcgccg acgccgcagt tgcgacatcc gccgcggacg cggtgcatga ggccggccgc
     3961 atctgggcga cctcgctttg ctgctagcgt tgggctcgat gaccggagaa gcgttccgcg
     4021 gcgcttcggc gcgcgtaccg gcagtcatcg agcatcgtga gtcccggccg gggcggattc
     4081 tccgccgaaa ccgggtgctg atcgcgtttg cgatcgccgc cgtggagggc gtcctcgtcc
     4141 tcacgggcac gctgccgtgg tggatcgtgc tcgcgctggc agcgggcgcg ctggccgtct
     4201 atctcgccgc cggacgcgac agcagagtgc ccgaggtccg tagcgt
//
LOCUS       DBNGNK010000219        10300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1617053,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000219 DBNGNK010000000
VERSION     DBNGNK010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10300
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1617053"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="AC150V_06465"
     CDS             complement(<1..295)
                     /locus_tag="AC150V_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3762601.1"
                     /translation="MTTMTDLEAPESRDVRALAEELDIRTRAWIDGDYVDAASGETFP
                     VINPGTGLEICSVAACAAEDVERAVAGARAAFESGEWSRAAPAQRKKILTRLAG"
     gene            complement(292..1437)
                     /locus_tag="AC150V_06470"
     CDS             complement(292..1437)
                     /locus_tag="AC150V_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3762602.1"
                     /translation="MQIRAAVLREPGHEVTIEPVELEPPRAREVLVRIAAAGVCHSDV
                     RLADAELGPGRWPIVLGHEGAGVVEAVGEGVGHVEPGDHVVFCFVPACRECRYCRMGK
                     PNLCAPAGENSLAGTLMDGTSRLRLPDGTPLQHALMTACFAERAVVASASAVKVPPEL
                     PLWQASLLGCSVVTAVGAVKNVAGVEPGASVIVIGCGGVGLQIVAAARRSGAETIVAV
                     DRVHEKLELALELGATHVVDGGRENPLPALLEATSGGASVAFEVIGGQETIRLAWDAV
                     RPGGTAVVVGLSPHGVEATVPAAEFISDKSLKGTYYGSGDAALELAELAVHAAAGKID
                     LGSVVTHIDSLDGVELALGRLRRSEGARTVLIVDAGLAGRKVEGGAK"
     gene            complement(1437..2546)
                     /locus_tag="AC150V_06475"
     CDS             complement(1437..2546)
                     /locus_tag="AC150V_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3762603.1"
                     /translation="MRITGIETREYRFELDPHLWVAWDPSPRIAQEATVVLVRTDEGL
                     TGVAGGDRLPDRELCERLLLGVDPRRTEIAREILETVDFHGARPWTLEAAVWDAAGKA
                     VGEPLWRMLGGRQERLLAYASAGERVPADERVRRCEALRDAGARALKLRFHHEDWRDD
                     VAVVRAVREAVGDELELMVDANQGWRMPGDRNGRWDVATAAQVARAVEPLGVYWLEEP
                     LRTDDLEGYRELRRLTSLRLAAGEMVRSPQEARDLVLRGGVDVIQTDAVLSLGVGGCR
                     RVAALADLCGRSWSPHTWSNGLGLVVNLHAAVALSTCPFLEVPLDPPGWSAERRDWLL
                     PAPLQIEPDGTIAPPPGPGLGVEPDFDALERFRIG"
     gene            complement(2543..3064)
                     /locus_tag="AC150V_06480"
     CDS             complement(2543..3064)
                     /locus_tag="AC150V_06480"
                     /inference="COORDINATES: protein motif:HMM:NF020297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HIR3762604.1"
                     /translation="MTPEELDGRFGEAWAGDVPNGSHINLVLARRASPTAAAAAASLA
                     EVRPGHAPFLACLGAGNRARPATIVRNKTTIDGDAIARLTWGAAQIGIGQGVADAVAE
                     GLIKEESVDEVVLLVAVWVDPAAHDHTSVRLANRRATRDAIADAVSQSYPDRVRAFVD
                     GRDSGTNDFYEGE"
     gene            complement(3061..4185)
                     /locus_tag="AC150V_06485"
     CDS             complement(3061..4185)
                     /locus_tag="AC150V_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3762605.1"
                     /translation="MRVQAAVLETTGGPLHVVELELEAPREGEVLVRLGATGVCHSDL
                     NAIDGTAETPCPAVLGHEGAGVVEVVGAAVTRVAPGDHVALSWAPSCGACAECLRDLP
                     WLCSTAWPAMATGGLLDGTSRLSRAGEVVHHYSFLSTFSEACVVPERSCIPIPADVPF
                     DVAGLVGCAVTTGMGAVWRTARVRPGDRVAVFGCGGVGMSALVAAVAVGAETIVAVDA
                     SAAKLELARTFGATEVVGWAGAADATAEAVREVSQGGVDYAIEATGRPEAMLSAFLST
                     RPRGAAVLIGIPREDDVLSLPALSIPRQERRVLGSVYGSSRPERDFAPILELYRAGRL
                     PLDQFVSHRLPLADVTEAFDLLQSGDALRVVLDLSLEEAP"
     gene            complement(4182..6218)
                     /locus_tag="AC150V_06490"
     CDS             complement(4182..6218)
                     /locus_tag="AC150V_06490"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762606.1"
                     /translation="MSDASGPSALRLHDLDVVYRVRGVELQVLRGLSLSVARGESYGL
                     VGESGCGKTTAAFAALRYLPRNGRVTGGLAEVAGKDVWKLSAAELRSLRANDVGIVYQ
                     NPSAALNPSLRVGEQVAEVFTVLGAGKNEASDRARQALVEVRIADPGSVLDRYPHQLS
                     GGMAQRVVIAMALAKDPSLLVLDEPTTGLDATVEAEVLELIEQLQAEHSTAILFISHN
                     LGVIAKMCERVGVLYAGRLVEEGTTQEVLSDARHPYTVGLLRCVPRGGLRKEDGRLDT
                     IPGFLPELGAQLDGCVFADRCGIARDECHREEPDLLRVQASHRSRCFFHEEAQQLPHQ
                     TEEGAVPATRAVDGDGPLIRFDGLTKVYSQHGDEIQALVDVSAAISAGETLGLVGESG
                     SGKTTLARALLGLVEPSIGTVALESRPLAAKLKGRSQDDVRALQIVFQNPAQALNRRH
                     SVRRILKRTLKKLSGLRGASADTRLERLTREVRLADRYLDAKPSQLSGGLQQRVAIAR
                     AFAGTPRLVVCDEPTSALDVSVQAAILNLLADLQAEERVAYLFISHDLGVVRYLSDRI
                     AVLYLGRLMEIGPAERVFSGPHHPYTEALLSAVPSIGGGRPSRIRLDGEIPSAASPPS
                     GCVFHTRCPRFVGDVCREEEPPLVDVESGHQMRCHIPIEELRRLQRPTEDEVPA"
     gene            complement(6215..7078)
                     /locus_tag="AC150V_06495"
     CDS             complement(6215..7078)
                     /locus_tag="AC150V_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3762607.1"
                     /translation="MSTVTATGGGRSAARRERARILLRSPAVLIGAGVLLFWVACAIL
                     GDLITPFDPIFDQTEKVFAAPSTEHWFGTDRLGRDVFSRVLVGSRDILIVAPAATLLG
                     TVLGTALGLVTGYFRGVVDDVTSRLIEAVLALPLIIVAVTALAALGSRSVWTVIAVIG
                     VVFTPIIARTVRAAVLAERELDYVQAARLRGERAPYIMFVEILPNVMGVVLVEATVRL
                     GYSIFAVAALTFINFGIQPPSPDWALQINEYYPFIDPNWWTVLFPAAAIASLIVAVNL
                     VADGIQQAYER"
     gene            complement(7075..8028)
                     /locus_tag="AC150V_06500"
     CDS             complement(7075..8028)
                     /locus_tag="AC150V_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3762608.1"
                     /translation="MTRYLLKRIGLALITLFLLSVIVFATAQLLPGDIGRSILGPFAP
                     QEAVDAFNADIGADRPAVVQYFDWIGGLFQGDFGESLALQIPVRPLLFDALWNSLKLA
                     LVAFVIVVPLGILGGLVAGLRAGKLSDRAITITGISLTTLPEFVTGIFLLLVFGIWLG
                     LLPISAEAPPGSGPFTQLKHLILPALALAFVLFGYIARITRASVIDSIDADYTRTAYL
                     KGLPSRVVLRRHVLRNALLPTIAVVATQVGYLVGGLVVIEILFNYNGVGLLIFNAAQQ
                     KDFTMLSSGVLVVGAIFLTVTLVADLLYAALNPRIRFGAVE"
     gene            complement(8110..9792)
                     /locus_tag="AC150V_06505"
     CDS             complement(8110..9792)
                     /locus_tag="AC150V_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3762609.1"
                     /translation="MDREDRRRIEAYRRDEAGPIENNLIDELVSGEVDRHEFLRRGAV
                     FGLSAGTMAALLGLVGEAAPAATLGSTAAKQGGTLRVAITKPVGTPEPSKAADVGSVG
                     VISAVVEYLTFSNARNQLKPWLATSWKPNKNASVWTFQIRQGVKFHTGATLTADDVVA
                     SMKVAASKDSAASSVFSGILAPSGVRKTGPYTVQFRLQEPTGGFPFLVSQTTYQAPIL
                     PKNYRVGTWIKSKMPGTGPYMFKSYVDGRSAEFAPNPDYWGAKPQLDGVTLTMFESTA
                     PQVLALRGGTVDLVQQLSVQEAQPFRTNTNFFVKSVGTANHRQFGLRVDDPAFRDPRV
                     RRAIALTLGRPEIVEKLFAGLASIGNDTPFWKGYPGSDRSTPQRTKNINLAKQLLNAA
                     GAKNLGFTITTWRNLEIPDYAQIIQSSAKQAGINVKLEIQTANEYYGAPQGADYATTT
                     PWLNRQATITDYGHRAIPNVYLTAALATGGVWNASKYANKQFDTAMKAYVAATDVPAQ
                     RKASKTMTKLLLRDTPVVFAYFYDYIAAGSTKVKGYTPEGLGALGLRGVSLA"
     gene            9997..>10300
                     /locus_tag="AC150V_06510"
     CDS             9997..>10300
                     /locus_tag="AC150V_06510"
                     /inference="COORDINATES: protein motif:HMM:NF020897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3762610.1"
                     /translation="MSETAREPVPDAIAEPGPPAATRRASSAGVQATLDVLELLVARG
                     PLTLSELSRELGLPKSTLHRVSTILAERAWVVRDEAGRFELGIRALAAGSQSYQLPL"
BASE COUNT         1654 a         3595 c         3521 g         1530 t
ORIGIN      
        1 gaccggcgag ccgtgtgagg attttcttgc gctgcgcggg ggcggcacgc gaccattcgc
       61 cggactcgaa cgcagcccgg gctccggcga ctgcacgctc gacgtcctca gcggcgcagg
      121 ccgcaacgct gcagatctcg agtcccgttc ccgggttgat caccgggaat gtctcgccgc
      181 tcgccgcgtc gacgtagtcg ccgtcgatcc aggccctcgt gcggatgtcg agctcctccg
      241 caagagctcg cacgtcgcgc gactcgggtg cctcgaggtc ggtcatggtc gtcatttggc
      301 tcctccttct accttccggc cggcaagtcc ggcgtcgacg atcagaactg tacgtgcgcc
      361 ttcactacgg cggagccgac ccagcgcgag ctcgaccccg tcgagcgagt cgatatgagt
      421 gacgaccgaa ccgagatcga tctttccggc cgcggcgtgc accgcgagct cggcgagctc
      481 gagtgccgcg tcgcccgacc cgtagtaggt ccccttcagc gacttgtccg agatgaactc
      541 ggcagccggc accgtcgcct cgacgccgtg aggcgagaga ccgaccacga ccgcagttcc
      601 gcccggccgg acagcgtccc aggccagccg gatcgtctcc tggccgccga tcacctcgaa
      661 cgctacgctc gcgccgccgc tggtcgcctc gagcagggcg ggcaggggat tctcgcgtcc
      721 accgtcgaca acgtgcgtcg ctccgagctc gagtgcgagt tcgagcttct cgtgcacccg
      781 gtcgacggcg acgatcgtct ctgcgccgga gcgacgggcc gccgcgacga tctggagccc
      841 aacgccgccg cagccgatga cgatcaccga cgcgcccggc tccaccccgg caacgttctt
      901 gacggcgccg accgcggtca ccacgctgca gccgagcagc gacgcttgcc agagtggaag
      961 ctccggcggc accttgaccg ccgacgccga cgcgacgacg gcgcgctcgg cgaagcaggc
     1021 cgtcatcagt gcgtgttgca gaggcgtccc gtccgggagc cgcaacctgc tcgtgccgtc
     1081 catcaacgtg ccggcgaggg agttctcgcc ggccggagcg cacaggttcg gcttccccat
     1141 ccggcagtac cggcactcgc ggcacgcggg cacgaagcaa aagacgacgt gatcgcccgg
     1201 ctcgacatgg ccgaccccct ccccgaccgc ctccaccacc ccggcgccct cgtgcccgag
     1261 cacgatgggc cagcgcccgg gccccagctc ggcgtccgcg agacgaacgt ccgagtggca
     1321 gacgccggcc gcggcgatcc gcacgagtac ctcccgcgcc cgcggcggct cgagctcgac
     1381 cggctcgatg gtcacctcgt gtcccggttc ccgcaagacc gcggcgcgga tttgcatcag
     1441 ccgatccgga aacgctcgag ggcgtcgaag tctggctcca cccccagtcc cgggccgggc
     1501 ggcggcgcga tcgtcccgtc gggctcgatc tggagagggg cgggcaggag ccagtcccgc
     1561 cgctccgcgc tccacccggg cggatcgagc ggcacttcga ggaacgggca ggtcgagagc
     1621 gccaccgccg cgtgaagatt caccacgagt ccgaggccgt tcgaccacgt gtgaggcgac
     1681 caggatcggc cgcagaggtc ggccagcgcg gccacgcgcc ggcagccgcc gacaccgagc
     1741 gaaaggacgg catcggtctg gatcacgtcg accccgccgc gaaggacgag atcacgggct
     1801 tcctgcggcg agcggaccat ctcacccgcc gccaatcgca ggctcgtcag gcgccgtagc
     1861 tctcggtagc cctcgaggtc gtccgtgcgg agcggctcct cgagccagta cacgccgagc
     1921 ggctcgactg cccgtgcgac ctgagcggcc gtcgcgacat cccagcggcc gttccggtca
     1981 ccgggcatgc gccagccctg gttggcgtcg accatcagct cgagctcgtc gccgactgcc
     2041 tcgcgcacgg ccctgacgac cgccacgtcg tcgcgccagt cctcgtggtg gaagcggagc
     2101 ttgagcgcgc gggcgccggc gtcgcggagc gcttcgcaac gacgcacacg ttcgtctgcc
     2161 ggcacgcgct ccccggcgga cgcgtaggcg agcaaccgtt cctgcctgcc cccgagcatt
     2221 cgccataggg gctcaccgac cgccttgccg gcggcatccc agaccgcggc ctcgagcgtc
     2281 cacggccgcg caccgtggaa gtcgaccgtc tcgaggattt cgcgcgcgat ctccgtgcgt
     2341 cgcgggtcca caccgaggag aagacgctcg cagagctcgc ggtccggcaa cctgtcgccg
     2401 ccggcgacgc cggtgagccc ttcgtccgtc cgcacgagca cgaccgtcgc ctcctgagcg
     2461 atgcgagggc tgggatccca ggcgacccag agatgaggat cgagctcgaa gcggtattcc
     2521 cgcgtctcga tccccgtgat cctcattcgc cctcgtagaa gtcgttcgtg ccggagtcgc
     2581 gaccgtcgac gaaggcccgt acgcggtctg gataggactg actcacggca tcggcgatcg
     2641 catcccgcgt cgcgcgccgg ttggcgagcc gcacggaggt gtgatcgtgg gccgccggat
     2701 cgacccacac ggcaacgagc aggacgacct cgtcgacgct ctcctccttg atcagccctt
     2761 ccgccacggc gtcggcgacg ccctggccga ttccgatctg cgcggcgccc caggtcaaac
     2821 gtgcgatcgc gtcgccgtcg atcgtcgtct tgttccggac gatcgtcgcg ggtcgtgcgc
     2881 ggttccccgc gccgagacag gcgaggaacg gtgcgtgccc ggggcgaacc tccgccaggg
     2941 aagccgccgc ggccgctgcc gtcggggagg cgcgtcgcgc gagcacgagg ttgatatggc
     3001 tcccgttcgg gacgtcaccc gcccacgcct cgccgaaccg tccatccagc tcttccggtg
     3061 tcacggggcc tcctccagag acaggtcgag gacgacacgc agcgcgtctc cggactgcaa
     3121 aagatcgaag gcttcggtca cgtcggcgag cggaagccta tgggagacga actggtcgag
     3181 cggaaggcga ccggcccggt agagctcgag gatcggggcg aagtcccgtt caggcctcga
     3241 cgagccgtag acggagccga ggacgcgccg ttcctgccgc gggatcgaca gcgcaggaag
     3301 agagagcaca tcgtcctcgc gcggaatccc gatcagcacg gcggcgccgc gcggccgggt
     3361 ggagaggaac gcggagagca tcgcctccgg ccgcccggtc gcctcgatcg cgtagtcgac
     3421 gcctccctgc gacacttccc gcaccgcctc ggcagtggcg tccgcggcgc cggcccaccc
     3481 cacgacttcg gttgcgccga aggtgcgagc cagctcgagc ttcgccgccg aggcgtcgac
     3541 tgcgacgatc gtctcggcgc ccacggcgac ggctgccacc aacgcggaca tccccacgcc
     3601 cccgcagccg aagaccgcca cccggtcgcc gggacgcact ctcgccgttc gccagaccgc
     3661 gcccatgccg gtcgtgacgg cgcagccgac gagcccggcg acgtcgaacg ggacgtcggc
     3721 ggggatgggg atgcacgacc gctcgggaac gacacacgct tccgaaaagg tggagaggaa
     3781 cgagtagtgg tgcacgactt cgcccgctcg cgaaaggcgg ctcgttccgt ccagcagccc
     3841 ccccgtggcc atcgccggcc acgccgtcga gcagagccac ggcaggtcgc gaagacactc
     3901 ggcgcaagcg ccacaggagg gggcccagga gagtgcgacg tgatcgcccg gcgccacgcg
     3961 cgtcaccgcg gcgccgacga cctccaccac accggcgccc tcgtgcccga gcacggcggg
     4021 gcacggtgtc tcagcggttc cgtcgatcgc gttcaggtcc gagtggcaga cgcccgttgc
     4081 gccgagccgt acgagcacct cgccctcgcg cggcgcctcg agctcgagct cgaccacgtg
     4141 gagcggccca cccgtcgtct cgagcacggc ggcctgcact ctcacgccgg gacttcgtcc
     4201 tcggtcggtc gctgcagcct ccgcagctcc tcgatcggaa tgtggcaacg catctggtga
     4261 cccgactcga cgtcgacgag cggcggctcc tcttcccggc agacgtcacc gacgaagcgg
     4321 gggcaccgcg tgtgaaagac gcagccggag ggtggcgaag ctgcggacgg gatctcgccg
     4381 tcgagccgga tgcggctcgg gcggccaccg ccaatcgacg gcacagcgga gaggagcgcc
     4441 tccgtgtacg ggtggtgcgg gccggagaag acccgttccg ccggcccgat ctccatcagg
     4501 cgtccgaggt agaggacggc gatccggtcg gacaagtagc ggacgacgcc gagatcgtgc
     4561 gagatgaaga ggtaagcgac gcgctcctcc gcctgaaggt cggccaggag gttgaggatc
     4621 gcggcctgta ccgagacgtc gagtgcagac gtcggctcgt cgcagaccac gagacgcggc
     4681 gtaccggcga acgcccgtgc aatggcgacg cgctgttgca gtccgcccga aagctgtgac
     4741 ggcttcgcgt cgaggtagcg gtcggcaagc cgcacctcgc gggtgagtcg ctccagccgg
     4801 gtgtccgcag acgcgccgcg aagcccggag agcttcttga gcgtgcgctt caggattcgc
     4861 cgaaccgagt ggcggcggtt gagggcctgc gcagggttct ggaacacgat ctggagcgcc
     4921 ctgacatcgt cctgcgaccg cccctttagc ttcgcagcga gcgggcggct ctccagagcc
     4981 accgtgccaa tgctcggctc gaccaacccg agcagcgccc gcgcgagcgt cgtcttgccg
     5041 ctgcccgact cgccgacgag cccgagcgtc tcgccggcgg agatcgccgc cgagacgtcg
     5101 accagcgcct ggatctcgtc gccgtgctgc gagtagacct tcgtcaggcc atcgaagcgg
     5161 atcagcggcc cgtcgccgtc gacggcccgc gtggcgggca cggcaccttc ctcggtctgg
     5221 tggggcagct gctgcgcttc ctcgtggaag aaacagcgac tccggtgcga ggcctggacg
     5281 cgaaggagat cgggctcctc gcggtggcac tcgtcgcggg cgatcccgca gcggtcggcg
     5341 aacacgcagc cgtcgagctg cgcgccgagc tccggcagga agcccgggat cgtgtcgagg
     5401 cggccgtcct ccttgcgcag cccgccgcgc ggaacgcagc gaagcaggcc gacggtgtag
     5461 ggatgacggg cgtccgagag cacctcttgc gtcgtgccct cctcgacgag ccggccagcg
     5521 tataggacac cgacccgctc gcacatcttc gcgatcactc cgaggttgtg gctgatgaac
     5581 agaatggccg tcgagtgctc tgcctggagc tgttcgatca actcgagtac ctccgcctcg
     5641 accgtcgcgt cgagcccggt cgtcggctcg tcgagcacga gcaggctggg gtccttcgcg
     5701 agagccatcg cgatcacgac ccgctgcgcc atcccgcccg agagctgatg cggatatcgg
     5761 tcgagcacgg agcccggatc tgcgatccgc acctcgacga gcgcctgccg ggcacggtcc
     5821 gacgcttcgt tcttgccggc cccgagcacg gtaaacacct ctgccacctg ctcgccgacg
     5881 cgcagcgagg gattcagcgc agcgctcggg ttctgataca cgatgccaac gtcgttcgcc
     5941 cgcagcgacc gcagctctgc cgccgacagc ttccagacgt ccttgccggc gacctccgcg
     6001 agcccgcccg tgacgcgacc gttccgcggc aggtagcgaa gcgccgcgaa ggctgccgtg
     6061 gtcttgccgc agcccgactc tccgacgagc ccgtagctct cgccgcgcgc caccgagaga
     6121 ctgagcccac gaaggacctg cagctctacc ccgcgcacgc ggtagacgac gtcgaggtcg
     6181 tgcagtcgga gagcggaggg gccggaagcg tcgctcatcg ctcgtaggcc tgctgaatgc
     6241 cgtcggcgac cagattcacg gcgacgatga gggaggcgat cgccgcagcc gggaagagga
     6301 cggtccacca gttcgggtcg atgaaggggt agtactcgtt tatctggagg gcccagtccg
     6361 gcgacggagg ctggatgccg aagttgatga acgtcagggc ggccaccgcg aagatcgagt
     6421 agccgagccg gacggtcgcc tcgacgagca cgacgcccat gacgttcggg aggatctcga
     6481 cgaacatgat gtagggagcg cgctctcctc gcagccgcgc ggcctgcacg tagtcgagct
     6541 cgcgctcggc cagcacggcg gcacgcacgg tgcgggcgat aatcggcgtg aagacgaccc
     6601 cgatcaccgc gatcactgtc cacaccgacc tgctgccgag cgccgcgaga gccgtcacag
     6661 cgacgatgat cagcggcagc gcgagcaccg cctcgatcag gcggctggtg acgtcgtcga
     6721 ccacccctcg gaagtaaccc gtgacgagac cgagggccgt cccgagcacg gttccgagca
     6781 gtgtcgcggc cggagcgacg atgaggatgt cgcgtgaccc gaccaagacg cgggagaaga
     6841 cgtcccggcc tagccgatcg gtgccgaacc agtgctcggt ggaaggcgcc gcgaacacct
     6901 tttcggtctg gtcgaagatc ggatcgaacg gcgtgatgag atcaccgagg atcgcgcaag
     6961 ccacccagaa gagcagcacg ccggctccga tcagcaccgc cgggctgcgg agcaggatgc
     7021 gcgcccgctc ccgtcgggct gcgctcctcc ctcccccggt cgccgtgacc gtgctcactc
     7081 gacggctccg aagcggatgc gcgggttgag agcggcgtag aggagatcgg cgacgagcgt
     7141 caccgtcagg aagatggccc cgacgacgag cacgccggag gagagcatcg tgaagtcctt
     7201 ctgctgtgcc gcgttgaaga tcaggagtcc gacgccgttg tagttgaaca gaatctcgat
     7261 cacgacgagg ccgcccacca ggtagcccac ctgggtggcc accacggcga tcgtcggcag
     7321 cagcgcgttg cgcagaacgt ggcggcggag gacgacgcga ctcggcagcc ctttcaggta
     7381 ggcggtgcgg gtgtagtccg catcgatcga gtcgatcacg ctggcgcggg tgatacgggc
     7441 gatgtagccg aacaggacga acgccagcgc gagggccggc aggatcagat gctttagctg
     7501 cgtgaacggc ccggagcccg gtggcgcttc cgcgctgatc gggagcagcc cgagccagat
     7561 gccgaagacg aggagcagga agatgcccgt cacgaactcg ggcaacgtcg tcagcgagat
     7621 gccggtgatc gtgatcgccc ggtccgacaa cttgccggcc cggagtcctg ccaccaggcc
     7681 gccgaggatc cccagtggaa cgacgatgac gaaggcgacg agagcgagct tcaacgagtt
     7741 ccagagcgcg tcgaagagaa gcgggcgaac ggggatctgt agggcgagcg actctccgaa
     7801 gtcgccttgg aacaggccgc cgatccagtc gaagtactgc accactgccg gcctgtcggc
     7861 cccgatgtcg gcattgaagg cgtccaccgc ctcctgcggc gcgaatggcc cgagaatgga
     7921 gcggccgatg tcgccgggga gcagctgtgc cgtcgcgaag acgatcacgc tcagcaggaa
     7981 gagcgtgatc agggcgaggc cgattcgctt caggaggtag cgggtgatgg ccgatcctcg
     8041 ccgatggcgg actggctggc gaccggcgcc gcttcgacgg cgccggtcgc gtgcgctcgg
     8101 agcgtggcgc taggcgagcg agacgccgcg cagtcccaga gcgccaaggc cctcaggtgt
     8161 ataccccttg accttggtcg atcccgccgc gatgtagtcg tagaagtacg cgaagacgac
     8221 cggcgtgtcg cggagcagca gcttcgtcat cgtcttcgag gctttccgct gcgcggggac
     8281 gtccgtggcg gcgacgtagg ccttcatcgc cgtgtcgaac tgcttgttcg catatttcga
     8341 cgcattccac acgccgcccg tcgcgagcgc agcggtgagg tagacgttcg ggatcgccct
     8401 gtgaccgtag tcggtgatcg tggcctgccg gttgagccac ggcgtggtgg tggcgtagtc
     8461 cgcgccctgc ggcgcgccgt agtactcgtt ggcggtctgg atctccagct tgacgttgat
     8521 ccccgcctgc ttggcggagg actggatgat ctgtgcgtag tcggggatct ccaggttccg
     8581 ccaggtcgtg atcgtgaagc cgaggttctt cgctcccgca gcgttcagga gttgctttgc
     8641 caggttgatg ttcttcgtcc gctgcggcgt cgagcgatcg ctcccggggt atcccttcca
     8701 gaaaggcgtg tcgttgccga tggaggccaa ccccgcgaag agcttctcga cgatctccgg
     8761 ccgcccgagc gtcagggcga tcgcgcgacg cacacgcggg tcgcggaagg cggggtcgtc
     8821 gacccgcagg ccgaactggc ggtggttcgc ggtgccgacg ctcttcacga agaagttcgt
     8881 gttcgtacgg aacggctgcg cctcttgcac cgagagctgc tgcaccagat cgacagtgcc
     8941 gccacgcagc gcgaggacct gcggcgccgt agactcgaac atcgtcaggg tgacgccgtc
     9001 gagctgaggt ttcgcgcccc agtagtcggg attcggcgca aactccgcac tccggccgtc
     9061 gacgtagctc ttgaacatgt acggaccggt gcccggcatc ttcgacttga tccaggttcc
     9121 gactctgtag ttcttcggga ggatcggggc ctggtaggtg gtctggctca ccaggaaggg
     9181 gaacccgccg gtcggctcct gcaggcgaaa ctgcaccgtg tacgggccgg tcttccggac
     9241 gccgctcggc gcgagaatgc ccgagaagac cgaggacgca gccgagtcct tcgaagccgc
     9301 gaccttcatc gacgcgacca cgtcgtcggc cgtcagcgtc gccccggtat ggaacttgac
     9361 gccctggcgg atctggaacg tccacacgct cgcgttcttg ttcggcttcc agctcgtcgc
     9421 cagccacggc ttgagctggt tcctggcgtt cgagaacgtc aggtactcga cgacggcgct
     9481 gatcacgccg acgctgccga cgtcagcggc cttgctcggc tcgggcgtgc cgaccggctt
     9541 cgtgatcgcc accctcaagg ttcctccctg cttcgcggcc gtcgagccga gcgtcgccgc
     9601 cggcgcggct tcgccgacga gcccgagcag tgcagccatc gtcccggccg agagcccgaa
     9661 cacagcgccg cggcgcagaa actcgtgcct gtcgacctca cccgagacga gttcgtcgat
     9721 caggttgttc tcgatcggcc ccgcctcgtc ccggcgatag gcttcgatcc tcctacggtc
     9781 ttcgcgatcc atcctctgct cctaaccgtg cgtcgaagac gtccgtgggc gtggaggcgg
     9841 ggtcgcttcc ttggcggcga acgtcgagtg cactgagtgt tctcgttgtg gacgtgtgtt
     9901 ccacgcctgc gcacggttat tgcacatagt ccgacctctg gtcaaacctg tgttcccgat
     9961 ctggatgccc gttccatagc aggctacgct tggcgggtga gcgagacggc acgcgagcct
    10021 gttcccgacg cgatcgccga accggggccg cccgccgcaa cgcggcgcgc cagctcggca
    10081 ggcgtccagg cgacgctcga cgtcctcgag ctgctcgtgg cccgaggccc gctgacgctc
    10141 tccgagctgt cccgtgagct cggcctcccg aagagcacgc tccaccgagt atcgaccatc
    10201 ctcgccgaac gggcctgggt cgtacgcgac gaggccggac ggttcgagct cgggatcaga
    10261 gctctcgcgg caggttcaca gtcgtatcag cttcccctcg
//
LOCUS       DBNGNK010000220         2911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1044041,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000220 DBNGNK010000000
VERSION     DBNGNK010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1044041"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="AC150V_06515"
     CDS             <1..740
                     /locus_tag="AC150V_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762611.1"
                     /translation="GLLVFGIRLVNRDYEERTDCVWIAFDVDRDADTGDTRLDGAEWT
                     LDYVSSRAILRKWNGSSWIDAPQAATVDSRLIRSLVVLYVNAREIGDPKVFDFRVKTM
                     ALAPHEGGVVLALDSAPARNARPWSYWVRTKRIALRASRLTRTPDPAKAGSPMIARMT
                     LTAFRGDVVERLGRNTRVVATAKVAGLTLAGKVAPSYRAGKIEVSFAVPEAAAGRLLR
                     GSVTVTYGGARVKRAFAVRVVASPRLS"
     gene            775..1881
                     /locus_tag="AC150V_06520"
     CDS             775..1881
                     /locus_tag="AC150V_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3762612.1"
                     /translation="MKIRAAVLEEFGAPLAVQEVDLAEPKPGEALVRLVACGVCHTDL
                     YTASGADPSGYAPTVLGHEGAGIVERVGEGVTDVVPGDHVVTLFSPQCRECVHCRSTK
                     TNLCLAIREQQNAGYLPDGTSRLGRDGNPIRHFMGTSTFAEYTVMPEIALAKVTSEAP
                     LEGACLFACGLSTGLGAAMNTARVEPGSTCVVFGAGMVGLGAVIGCRLQGAERIVCVD
                     LSADRLELARTHGATDVLVGGDGVVDQILELTGGFGADYTFEATGNVTVMRQAVESAR
                     MGWGLCTVAGVAGKGEVLEVVPRFLITGRRVAGSSFGGVKGRDQVPQLVERWLAGEID
                     VESFISHRITLEDVNLGFELMEAQDGIRSVIQFS"
     gene            complement(1895..2599)
                     /locus_tag="AC150V_06525"
     CDS             complement(1895..2599)
                     /locus_tag="AC150V_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3762613.1"
                     /translation="MDIQEMLNSIFDADTGIYQQRGFQRRIGFGARPALIHIDLANAW
                     TRAGNAFSCDGMDTIIPAVQRLNEAGRAKGIPIVYTTTAYQDVDGPNSDMGLWIHKIP
                     VEHLKVGTEAVEIDDRIAPQPGEMVLVKKRASGFHGTNLTSYLNAHGVDTVIITGVTM
                     AGCVRHTTEDAIAEGFRPIVVREGVGDRVPGVVEWNLFDIDAKFGDVEPLERVLEYLE
                     GIEQFESRSAAAVARA"
     gene            complement(2644..>2911)
                     /locus_tag="AC150V_06530"
     CDS             complement(2644..>2911)
                     /locus_tag="AC150V_06530"
                     /inference="COORDINATES: protein motif:HMM:NF020253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR3762614.1"
                     /translation="LSCLVVDVDGADAHGFEPLYTAGGSEPVAYVSSGGYGHTIEVSI
                     ALAYLPVAHATVGTRLEVCILGERRRAVVTEQPLYDPLNERLRS"
BASE COUNT          459 a          947 c         1028 g          477 t
ORIGIN      
        1 caggactgct cgtgttcggg atccgcctcg tcaaccgcga ctacgaggag cgcaccgact
       61 gcgtctggat cgcgttcgac gtggaccgcg acgccgacac cggcgacacc cggctggacg
      121 gcgccgaatg gacactcgac tacgtatcct cgcgggccat cctcagaaag tggaacggca
      181 gcagctggat cgatgccccg caggcggcga cggtcgactc gcggctgatc cggagcctcg
      241 tcgtcctcta cgtcaacgcg cgggagatag gcgacccaaa ggtattcgac ttcagagtca
      301 agacgatggc gctcgccccg cacgaaggcg gcgtcgtcct ggctctcgac tcggctcccg
      361 cgcggaacgc gcgcccctgg agctactggg ttcgcacgaa gaggatcgcc ttgcgagcgt
      421 cgaggctgac ccgcacgccc gatccggcaa aggcggggag tccgatgatc gccaggatga
      481 cgctgaccgc gttccgcggc gacgtcgtcg agagactcgg gcgaaatact cgcgtggtcg
      541 ctacggcgaa ggtcgccggc ttgacgctgg ccggaaaggt cgctccgagc tacagggccg
      601 gcaagatcga ggtgtcgttc gccgtccccg aggcggcggc gggcaggttg ctgcggggct
      661 cggtgacggt cacctacggc ggtgccaggg tcaagcgcgc cttcgcggta cgggtcgtcg
      721 cgtcgccccg cctcagctga gcgaccaccc tgcgcgccgt gagaggatga agcgatgaag
      781 atccgcgcgg cagtgctcga ggaattcgga gctcctctcg cggtgcagga ggtcgacctg
      841 gccgagccga agccgggcga ggcgctcgtg cgcctggtcg cctgcggcgt gtgccacacg
      901 gatctctaca cggcctccgg cgccgacccg tccggctatg cgccgacggt gctcggccac
      961 gagggcgccg gcatcgtcga gcgtgtcggc gagggagtca ccgacgtggt tcccggcgat
     1021 cacgtcgtca ccctcttctc gccccagtgt cgagagtgtg tccactgccg gagcacgaag
     1081 acgaacctct gcctggcgat ccgcgagcag cagaacgccg ggtacctgcc cgacggcacg
     1141 agccggctgg gccgggacgg caatccaatc cgccacttca tggggacgtc gaccttcgcc
     1201 gagtacacgg tgatgccgga aatcgcgttg gccaaagtca cgtccgaggc gccactggag
     1261 ggcgcgtgcc tgtttgcgtg cgggctgtcg acgggattgg gagcggcgat gaacactgcc
     1321 agggtggagc cgggctcgac ctgcgtcgtg ttcggggccg gcatggtcgg actcggcgcc
     1381 gttatcgggt gccggctgca gggcgccgag cgcatcgtct gcgtcgacct ctccgccgac
     1441 cggctcgagc tggcgcgaac acacggcgca acggacgtcc tcgtaggcgg cgacggcgtc
     1501 gtcgaccaga tcctggagct caccggcggg ttcggcgccg attacacgtt cgaggccacg
     1561 gggaacgtga cggtcatgcg tcaggcggtg gagtccgcgc gcatgggctg gggcctgtgc
     1621 acagtcgccg gcgtcgcggg caagggagag gtgctggagg tggtgcctcg cttcctgatc
     1681 accggcaggc gcgttgccgg ctcgtcgttc ggcggcgtca agggacgcga tcaggtgccg
     1741 cagctcgtcg agcgctggct cgccggagag atcgacgtcg agtcgttcat ctcacaccgc
     1801 atcactctcg aagacgtcaa ccttggtttc gagctgatgg aagcgcagga cgggatccga
     1861 agcgtgatcc agttctcctg acgacccttt atctctaggc tcgcgcgacc gcggccgcgc
     1921 tgcgactctc gaactgctcg atgccctcca ggtactcgag cacgcgctcg agtggctcga
     1981 cgtcgccgaa cttggcgtcg atgtcgaaga ggttccactc gacgacgccg ggtacccggt
     2041 cgcccacgcc ctcacgcacg acgatcgggc gaaagccctc cgcgatcgca tcctcggtcg
     2101 tatggcggac gcagccggcc atggtcacgc cggtgatgat caccgtgtcg acgccgtgcg
     2161 cgttcaggta gctggtgagg ttggtgccat ggaagccgct tgcccgcttc ttcaccagca
     2221 ccatctcccc cggttgcggc gcgatgcggt cgtcgatctc gacggcctcg gtgccgacct
     2281 tcagatgctc gacagggatc ttgtggatcc agagccccat gtcggagttc gggccgtcga
     2341 cgtcctggta tgcggtcgtc gtgtacacaa tcgggatacc tttggcccgt cccgcctcgt
     2401 tcaggcgctg aacggcgggg atgatcgtgt ccatgccgtc acacgagaag gcgttcccgg
     2461 ctctcgtcca ggcgttcgcc aggtcgatgt ggatgagcgc agggcgggcg ccgaagccga
     2521 ttcgacgctg gaagccgcgc tgctggtaga tgccggtgtc ggcatcgaag atcgagttga
     2581 gcatctcctg gatgtccatc tggcctcctt cggatcggac cagagcatac gacggagacg
     2641 cggctacgag cgaagccgct cgttgagggg gtcgtagagc ggctgctcgg tgaccacggc
     2701 tcgtcggcgc tcgccgagga tgcaaacctc gagccgagtg ccgacggtgg cgtgcgcgac
     2761 cggcagatag gcgagcgcga tcgagacctc gatcgtgtgg ccgtaccccc cggacgagac
     2821 gtacgcgacc ggctcgctgc cgccggctgt gtagagaggc tcgaagccgt gcgcgtcggc
     2881 gccgtcgacg tccacgacca ggcaggacag g
//
LOCUS       DBNGNK010000221         3398 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1115616,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000221 DBNGNK010000000
VERSION     DBNGNK010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3398)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3398
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1115616"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="AC150V_06535"
     CDS             <1..219
                     /locus_tag="AC150V_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3762615.1"
                     /translation="KGELKEALGEAISRLPEREKLVVTLYYYEELTLREIGEVLGVTE
                     SRVSQLHTKAVLRMKARLSGGAREPAGT"
     gene            316..2367
                     /gene="fusA"
                     /locus_tag="AC150V_06540"
     CDS             316..2367
                     /gene="fusA"
                     /locus_tag="AC150V_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR3762616.1"
                     /translation="MAATEPGKIRNVAVVGHRGTGKTSLVEALLYQSGAVNRLGTIES
                     GTTVADWDEDEHQRQMSIALSLCHATWQDRKINFVDVPGDPGFQGEVRCALPVVEGAL
                     VVASAVMGVEVGTVRAWRIADDLGLPRLLFVNMLDRERADFFRTLEQLQASLSPHCVA
                     VHLPIGAEHELTGIVDVLHMCAYQSPEGEKEGEPGPIPDELGDLVAGYREKLLDAVVE
                     TDEALMERYLEGEELDAHEVTAALKAAVTRGEVFPVACGVATKNLGTHALLDLIVEGI
                     PSPAKRGATLSVDGAATAAFVFKTMADPFAGKINVFRVLTGTVTGDTTLIDQRERAKE
                     RMGSLLQLQGKEHTPVKEFGEGDLGAVAKLKDVQTGDLLLDSDLGADAIDLQFPEPVM
                     SFAITPKAKGDEEKVANAIRRLAEEDPTLHLRRDQQTGEEILSGMSQMHVEVALDRAN
                     RRFGVEIELHPPRVPYLETIRQEARARHRYKKQTGGRGQFGDCAIVIEPIPGNTGYEF
                     VDEIVGGVISQSYRPAVDKGIQEAMQQGELAGAPVQGVRVRLVDGQEHQVDSSEMAFK
                     IAGSMAFKEAYQKAGPVLLEPIMEVEVSVPDEAVGAVNGDLNSRRGRLQGMEPAGGMT
                     SIKAEVPLAEVLTYSQSLTSMTGGRGDYSMSFVRYEEVPTHVAQKIIEETRRAGEEEE
                     S"
     gene            2628..3233
                     /locus_tag="AC150V_06545"
     CDS             2628..3233
                     /locus_tag="AC150V_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762617.1"
                     /translation="MRVTKGNVARSCAVCERSLLMGEHMFRFSPDGHEYLDVCDLCQD
                     AALGNGWVREGSSLSPAVSPPPRGRSLFASLLGGGRDDPQPIVSEPILRRLSDRELAL
                     VEAADLFNATQFRRTIASVSRALGPPQVSIVPLSGVNAEVVMTFAWEITWYQYRIAPD
                     SGQPVRLSERGHDIEELEASAMSWNAQVDEDGRVVPAIEPL"
     gene            3245..3382
                     /locus_tag="AC150V_06550"
     CDS             3245..3382
                     /locus_tag="AC150V_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762618.1"
                     /translation="MATATRQYRDYMGGGLVDVLDVFTERSAVDVDYSGRLQRAQIDD
                     Q"
BASE COUNT          582 a         1118 c         1187 g          511 t
ORIGIN      
        1 aaaggcgagc tgaaggaggc gctcggcgag gcgatctccc ggctcccgga gcgcgagaag
       61 ctcgtggtca ccctctacta ctacgaagag ctgaccctac gcgagatcgg cgaggtgctc
      121 ggcgtcaccg agtcgcgtgt ctcgcagctc catacgaagg ccgtgctacg catgaaagct
      181 cgcctctcgg gcggtgcccg agagcccgcc ggcacgtagg agcggcgcgc agggcgtccg
      241 tacccgcgga gcaggctggc ggctgcgact aagatcgcgc tcgcgagacc gaaccgagga
      301 ggagagcacg aacgtatggc tgccaccgag cctgggaaga ttcgcaatgt cgccgtcgtc
      361 ggacaccggg gaacgggcaa gacgtccctc gtagaggcgc tgctgtatca gagcggcgcg
      421 gtcaatcggc tcggcacgat cgagagcggg acgaccgtcg ccgactggga cgaggacgag
      481 caccagcggc agatgtccat cgcactctcc ctctgccacg cgacgtggca ggaccgcaag
      541 atcaacttcg tcgacgtccc cggcgacccg ggcttccagg gcgaggtccg gtgcgcgctg
      601 ccggtcgtcg agggcgcgct cgtcgtcgcg agcgcggtca tgggcgtgga ggtggggacg
      661 gtccgggcgt ggcggatcgc cgacgatctg ggactgccga ggctcctttt cgtcaacatg
      721 ctcgaccgtg agcgcgccga cttcttccgc acgctcgagc agctgcaggc gagcctctcg
      781 ccgcactgcg tggccgtcca tcttccgatc ggtgccgagc acgagctgac cggaatcgtc
      841 gacgtgctgc acatgtgcgc gtaccagagc ccggagggcg agaaggaagg ggagcccggg
      901 ccgattcccg acgagctcgg agacctcgtg gcgggctacc gcgagaagct cctcgacgcc
      961 gtcgtggaga ccgacgaggc gctgatggag cgctatctcg agggtgagga gctcgacgcc
     1021 catgaggtaa ccgccgcgct caaggcggcc gtcacacgag gcgaagtctt ccccgtcgcg
     1081 tgcggcgttg cgaccaagaa cctcggcacg cacgcgctcc tcgacctgat cgtcgagggg
     1141 attccgtcgc cggcgaagcg cggcgcgacg ctgtccgtcg acggtgcggc gacggcggcg
     1201 ttcgtcttca agacgatggc cgaccccttc gccggcaaga tcaacgtctt ccgggttctg
     1261 accggaacgg tcaccggcga tacgacgctg atcgaccagc gggagcgcgc gaaggagcgc
     1321 atgggctctc tgctccaact gcaggggaag gagcacacgc ccgtcaagga gttcggcgag
     1381 ggcgacctcg gcgcagtcgc caagctgaag gacgtccaga ccggcgatct cctgctcgac
     1441 agcgacctcg gcgccgacgc gatcgacctc cagtttcccg agccggtgat gagtttcgcc
     1501 atcacgccga aggcgaaagg cgatgaggag aaggtcgcaa acgccatccg gcgtctcgcc
     1561 gaggaggacc cgacgcttca tctgcgtcgc gaccagcaga cgggcgagga gatcctctcg
     1621 ggcatgagcc agatgcacgt cgaggttgcg ctggaccgtg ccaatcggcg ctttggcgtc
     1681 gagatcgagc ttcatccgcc gcgggtgccc tacctggaga cgatccggca ggaggcgcgc
     1741 gcacgccatc gctacaagaa gcagacgggc ggccgcggcc agttcggcga ttgcgccatc
     1801 gtcatcgagc cgattcccgg caacacgggc tacgagttcg tcgacgagat cgtcggcggc
     1861 gtcatctcac agagctaccg gccggcggtc gacaagggga tccaggaggc gatgcaacag
     1921 ggagagctcg ccggggcccc cgtgcaggga gtgcgcgtcc gactggtcga cggtcaggag
     1981 caccaggtcg actcgtcgga gatggcgttc aagatcgccg gctcgatggc cttcaaggag
     2041 gcctatcaga aggccgggcc cgtgcttctc gagccgatca tggaggtcga ggtttcggtg
     2101 ccggacgagg ccgtcggggc cgtcaacggc gacctcaact ctcgcagggg ccggctgcag
     2161 gggatggagc cggcaggagg gatgacctcg atcaaggctg aggttccgct ggccgaggtc
     2221 ctcacctact cgcagtcgct cacctcgatg accggtggtc gcggcgacta ctcgatgagc
     2281 ttcgtccgct acgaggaagt gcccactcac gtcgcccaga agatcatcga ggagacacgc
     2341 cgggccggcg aggaagaaga gtcgtagggg gcagcagcgg cacgccgtcg ccgacgcgga
     2401 gcaccgtttc tccacagcgt cggcacggcc cgatcggaca cgacccgcct ctgaggtccg
     2461 acgtggctgc ggtggaccag gtcgatgcgg acaggagctg caggcgttga ttcgttgtcg
     2521 caccctcgtc ctgcccgcac cggacagggc cggtccccag agcaccggac gtgtccgccc
     2581 gacggctccc tgtggataac ttcggcggcg gtgctacgct cgcgcagatg cgggtaacca
     2641 agggaaacgt cgcgcgctcc tgcgccgtct gcgagcgctc cctgctcatg ggtgagcaca
     2701 tgtttcgctt ctcgcccgac gggcacgagt atctcgacgt ctgcgacctc tgccaggacg
     2761 ccgcgctggg caacggctgg gtccgcgagg gtagctccct ctcacccgcg gtgagcccgc
     2821 cgccgcgcgg tcgctccctc ttcgcgtcgc tcctcggcgg agggcgcgac gatccacagc
     2881 ccatcgtctc ggagccgatc ctgagaaggc tctcggaccg ggagcttgca ctcgtcgagg
     2941 ccgccgacct cttcaacgcg acgcagttcc gccggacgat cgccagtgtc tcgcgagcgc
     3001 tcgggccgcc tcaggtgtcg atcgtgccgc tctccggcgt caacgccgag gtcgtgatga
     3061 cgttcgcctg ggagatcacc tggtaccagt accgcatcgc tcccgactcc ggccagccgg
     3121 ttcggctctc cgagcgcggc cacgacatcg aggagctcga ggcgtcggcg atgagctgga
     3181 acgcgcaggt cgacgaggac ggacgcgtcg ttcccgccat cgagccgctc tagactcgtt
     3241 ctcggtggca acagcgaccc ggcaataccg tgactacatg ggtggcgggt tggtcgatgt
     3301 gctcgacgtc ttcaccgagc ggagtgcggt cgacgtcgac tactccgggc ggctccagcg
     3361 ggcgcagatc gacgaccagt agtggacgac aaggtcaa
//
LOCUS       DBNGNK010000222         2486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1472593,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000222 DBNGNK010000000
VERSION     DBNGNK010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1472593"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="AC150V_06555"
     CDS             complement(<1..377)
                     /locus_tag="AC150V_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762619.1"
                     /translation="MAQPQQQHATPELPLHPQDEVECRRCDVHCDKVVYPGACIENAC
                     PFVYAYEACGHTYMGCMQKVFEVEIDLDLLRAAERRRDGFGAVRARRAPLPMCSVEIS
                     SCYEHRGDELGCRNPEFHEMPLSR"
     gene            495..1592
                     /gene="cax"
                     /locus_tag="AC150V_06560"
     CDS             495..1592
                     /gene="cax"
                     /locus_tag="AC150V_06560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HIR3762620.1"
                     /translation="MVRRILFASLALAPITIGLGYFSGLDDTTLFVFSALALIPLAWL
                     IGEATEHAAEHTGPGIGGFLNATFGNAPELIIALIAVNQGLTEVVRGSLTGSVIGNLL
                     LVLGFSLVFGGRGELDRYSSFLSFTLIGGAVVLLLIPSVPGWDGDPDTHGLALLSIPV
                     SIVLFLLYVGATWFALRRHHEIHVASDEAIKAWSLRAALVALAVTTLFTALVAELLVG
                     TLESFAETAGLSEFFVAAVIVAIVGNAAEHGGAVVVAYRGKIKLAAEIALASAAQVAV
                     FLIPAVALLSWLIDPLSLSFRPIEIAALGGALVFTTLVLSDGRSSRFRGALLLSAYVA
                     IAVAFFFAGDRGDHHDSPNAEGARVSGHVAK"
     gene            complement(1637..>2486)
                     /locus_tag="AC150V_06565"
     CDS             complement(1637..>2486)
                     /locus_tag="AC150V_06565"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR3762621.1"
                     /translation="LKLHSRPGDVVVAERHSHVVVYEYGGAAAHAGLMVEALPGAAGV
                     LTAEQIVAATQPSAKAADQRAVLLSLEDTHNNSGGRVWPLAALDDVVAAAREIGLAVH
                     LDGARLLNAAVALGVEPAEIASRFDTVTLCLSKGLGCPLGALIAGSETLMERAWREKH
                     LFGGAMRQAGIAAVAGLYALDHNVRRLAEDHEHARRLARAWADAGLPVDLAQVESNFV
                     QLDVGRIGLTSGEALALMNASGVALSTTIHPAVLRAVTHLDVSSEDIEAACELVPEAL
                     QSRVGA"
BASE COUNT          331 a          852 c          857 g          446 t
ORIGIN      
        1 cgcgacagcg gcatctcgtg gaactcgggg ttgcggcagc ccagctcgtc gcctctgtgc
       61 tcgtagcagg aggaaatctc gacggagcac atggggagcg gtgctcgacg ggcccgtacg
      121 gctccgaagc cgtcgcgacg acgctcggcg gcccgcagca ggtcgaggtc gatctccacc
      181 tcgaagacct tctgcatgca gcccatgtag gtgtggccgc acgcttcgta ggcgtagacg
      241 aacgggcagg cgttctcgat gcacgcgcct gggtagacga ccttgtcgca gtgcacgtcg
      301 caacgtcggc actcgacctc gtcctgcggg tgcaggggga gctccggggt ggcgtgctgc
      361 tgttgcggtt gtgccatcta cagagattgt ggaacccggg aggcgttcag cgcaagactt
      421 gacaaacttc tcgttttccg gcaaagggcg gctgcggccc gctagcggat gagccggata
      481 gtgttgcctc ttccgtggtt cgccgcatcc ttttcgcatc tctggccctg gcgccgatca
      541 ctatcggcct gggctacttc tcgggtctcg acgacacgac gctgttcgtg ttctcggcgc
      601 tggcgctgat cccgctcgcc tggctcatcg gcgaagccac ggagcatgct gccgagcaca
      661 cggggccggg gatcggcggt ttcctcaatg cgaccttcgg gaacgcgccg gagctgatca
      721 tcgccctgat tgcggtgaac cagggcttga ccgaggtcgt gcgcggatcc ctgacgggct
      781 cggtgatcgg caacctcctg ctggtgctcg gcttctcgct cgtcttcggc ggccgcgggg
      841 agctcgatcg ttactcgagc ttcctgtcgt tcacgctgat cggcggcgcc gtcgtcctct
      901 tgctcattcc ctcggtaccg ggctgggacg gcgaccctga cacccatggt ctcgcgctcc
      961 tctccatccc cgtctcgatc gtgctgttcc tcctgtacgt gggagcgacg tggttcgctt
     1021 tgcgacgcca tcacgagatc cacgtcgcca gtgacgaggc gatcaaggcc tggtccttgc
     1081 gcgcggcgct cgttgcactc gccgtcacca cgctgttcac ggcgctcgtc gccgagttgc
     1141 tcgtcggcac gctcgagtcg ttcgccgaga ccgcggggct gagcgagttc ttcgtcgcgg
     1201 ccgtcatcgt cgcgatcgtc ggcaacgctg ccgaacacgg cggtgccgtc gttgtcgcct
     1261 accggggcaa gatcaagctg gcggcggaga tcgcgctggc gtccgcagcg caggtcgccg
     1321 tcttcctgat tcccgcggtt gccctgctct cgtggctgat cgatccgctt tcgctcagct
     1381 tccgcccgat cgagatcgcg gcgctcgggg gagcgctcgt gttcacgact ctcgtgctct
     1441 cggacggacg gtcgagccgc ttccgcggcg cgttgttgct cagtgcgtac gtcgcgatcg
     1501 cagtcgcctt cttcttcgcg ggcgaccgcg gcgaccatca cgactcgccg aacgccgagg
     1561 gcgcccgcgt cagcgggcac gtcgcgaagt aaggccggcg tcctcgccgt gcggttgcgc
     1621 accggtcggc cggttttcag gcgccgacgc ggctctgcag cgcttccggg acgagctcgc
     1681 atgccgcctc gatgtcctcg gaggagacgt ccaggtgggt cacggcgcgg agcacggcag
     1741 gatggatcgt ggtcgagagc gccaccccgg acgcattcat gagcgcgagc gcctccccgc
     1801 tcgtgagtcc gatccggccg acgtcgagct gcacgaagtt cgactccacc tgggcaagat
     1861 cgaccggaag gccggcgtct gcccatgcgc gcgccagtcg gcgcgcgtgc tcgtgatcct
     1921 cggccagccg ccgcacgttg tggtcgaggg catagaggcc cgcgactgca gcgatgcccg
     1981 cctgccgcat ggcgcctccg aacaggtgct tctcgcgcca ggcgcgctcc atcagcgtct
     2041 cggagccggc gatgagggcg ccgagcgggc agccgaggcc cttcgagagg cagagcgtga
     2101 cggtgtcgaa cctggacgcg atctccgcgg gctcgacgcc gagtgcgacc gcggcgttca
     2161 gcaggcgggc gccgtccagg tgaacggcca gcccgatctc acgcgccgcc gcaacgacgt
     2221 cgtcgagcgc ggccaggggc cagacccgcc cgccggagtt gttgtgcgtg tcctccagcg
     2281 acagcaggac ggcgcgctgg tcggccgctt ttgcgctggg ctgcgtcgca gccacgatct
     2341 gctcggccgt gaggacgccg gccgccccgg gcagcgcctc gaccatcagg cccgcgtgcg
     2401 cggcggcgcc gccgtactcg tagacgacga cgtgcgaatg gcgctccgcg acgactacgt
     2461 cgcccggacg ggagtgcagc ttcagc
//
LOCUS       DBNGNK010000223         4946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_612941,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000223 DBNGNK010000000
VERSION     DBNGNK010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_612941"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..672)
                     /locus_tag="AC150V_06570"
     CDS             complement(130..672)
                     /locus_tag="AC150V_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762622.1"
                     /translation="MSKRSLSRALPWFAGLVLVAGLVAFGTVYIFDGGETVEVQEPGE
                     TGDTAVVNTTPKNVPLDPEARKVAGKFIVTAVTREKLGESYKITHPELRAGYTLEEWK
                     AGDIPVQYYPADAIDKATFKIDESYTDEAVLEVALLPKEGEEIKPQIFYIGLKKVNGK
                     WLVYYWAPRSAIPVPATGDG"
     gene            complement(731..2395)
                     /locus_tag="AC150V_06575"
     CDS             complement(731..2395)
                     /locus_tag="AC150V_06575"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3762623.1"
                     /translation="MATTEDRDAVGGLRTWRKRFDATPPREGELTTTISGLENEPLHT
                     PETVDIDYERDLGYPGAYPLTRGVYPSMYRGRLWTMRQFAGFGTAEETNERFHYLLEH
                     GQTGLSTAFDMPTLMGYDSDHARAVGEVGREGVAIDSLADMESLFDGIPRADVSPSMT
                     VNAPAAMLLAFYLCVGEQQGADRAALRGTIQTDILKEYIAQKEYIFPPEPSMRLVTDM
                     IEFCAREMPLWHPISISGYHIREAGSTAVQELAFTLQDGFTYVEWALERGLEIDDFAP
                     RLSFFFNAHLDFFEEIAKYRAARRIWARELRERYGATNEQSCLMRFHAQTAGVSLTAQ
                     QPEVNIVRTAIEALAAVLGGTQSLHTNSYDEALALPTEDAVRIALRTQQVIAHETGVV
                     NAIDPLGGSYYVEHLTNELERGAYEIFQRIEDAGGMVEAIKLNIPQRDIAEASFRYQA
                     EVEAGHRVVVGVNRYAAEGDEQIPILRIDPALEAKQIERVRALRSRRNAAEVEAALAG
                     VHRAAAREDVNLMPVIVDAARTYVTMGEMCDALRDVWGTWRETPVF"
     gene            2488..3228
                     /locus_tag="AC150V_06580"
     CDS             2488..3228
                     /locus_tag="AC150V_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR3762624.1"
                     /translation="MTFSARAAALVAVLAATVATGATAHAASSCGGGSHGKQDYGYGG
                     HQAAKGTSGIRAAITATRTPSVDDGHVAGWVGVGGPGAGPGGRDQWLQVGLAALPGSG
                     VLLYTEVTRAGREPVFRLVELDVTPGATRTVIVRETARRGWWQASVDGRPVTKPVHLS
                     GMQGVRPIVTTEAWNPGSRSCNRFSFRFDDVSVVAPGGGSWRPFVPGLHFRDSGFSVR
                     AVKPRAASGDRALAAQNRASFAFLASAG"
     gene            complement(3225..3944)
                     /locus_tag="AC150V_06585"
     CDS             complement(3225..3944)
                     /locus_tag="AC150V_06585"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR3762625.1"
                     /translation="MATTRFVFALFGISLLAVVLARDASPSDAAGSARVEVVGSGTLT
                     QPRVVVRARPTNTARRVAVLTQFRKSFDPRVVLAVAERLHPSTGGPAWYRIALPGRPN
                     GRTGWIRAAQLELEPVDRKVIIYRETRRFEFWIEDRLVRRGKVAVGARGMETPLGLFY
                     VTAKFKPSWSVLGAFAFETSAYSKLSEWPGGGVVGLHGTNAPHLLGQAVSHGCVRLHD
                     RDALFLRSRVSVGTPIKVVRG"
     gene            complement(4086..4850)
                     /locus_tag="AC150V_06590"
     CDS             complement(4086..4850)
                     /locus_tag="AC150V_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HIR3762626.1"
                     /translation="MIQSTPRIDVGVAKWDRPDPVQVGKTITYTLRVVNNGPNRAIDV
                     QVADPAPTGIAFLSASPSKGTCRVSAALVQCDLGSLGKGETIEVTVIARAERTGLVTN
                     TATVTGSGGAEVNPGDNTASTTTLVVAPATPPKSTVKPKPKPKQPQAKPKPPVRKPTI
                     CSTVKVTKKLLRATGKRQRFKVRVTAGGKPVARAAVRIKGPGVFKIVRTGKTGVIVVT
                     LKPRKAGILTVSVRNKKGCNTQRIGVVGIFQPPVTG"
BASE COUNT          728 a         1688 c         1728 g          802 t
ORIGIN      
        1 acctcttgct catcgcgaca agggtagacg accgcgacgg tggaggctgc gccgagcgcg
       61 agtccgaccc gcagcgcgcg gcggcggaag cggggcgaag aaagcctgcc cgccaaccgc
      121 cgaacggccc tatccgtcgc cggtcgccgg aacgggaatg gccgagcgag gcgcccagta
      181 gtacaccagc catttcccgt tcaccttctt gaggccgatg tagaagatct gcggcttgat
      241 ctcctcgcct tccttcggca ggagcgcgac ctcgagcact gcctcgtccg tgtacgactc
      301 gtcgatcttg aatgtcgcct tgtcgatcgc gtcggccggg tagtactgga ccgggatgtc
      361 cccggccttc cactcctcga gcgtgtagcc ggcccgaagc tcggggtggg tgatcttgta
      421 cgactcgccg agcttctcgc gggtcacggc tgtgacgatg aacttgcccg ccaccttgcg
      481 tgcctcggga tcgagcggaa cgttcttcgg tgtcgtgttg acgaccgccg tgtcgccggt
      541 ctcgcccggc tcctgcacct cgacggtctc accgccgtcg aagatgtaga ccgtgccgaa
      601 ggcgacgagc ccggcgacga gaacgagccc ggcgaaccag ggcaatgcgc ggctgagaga
      661 cctcttgctc atcgcgacaa gggtagacga ccgcgattaa gcgccggtta gcggatcgta
      721 cacccgccgt tcagaagaca ggcgtctcgc gccaggtgcc ccagacgtca cgaagggcgt
      781 cgcacatctc ccccatcgtc acgtaggtgc gcgctgcgtc gacgatcaca ggcatcaggt
      841 tcacgtcctc gcgggcggcg gcgcggtgta cccccgcgag agccgcttcg acctcggcgg
      901 cgttgcgccg ggagcgcaag gctcggacgc gctcgatctg cttcgcctcg agtgccgggt
      961 cgatgcgcag gatcgggatc tgctcgtctc cctcggccgc gtagcggttc accccgacca
     1021 cgacgcggtg gcccgcttcg acctccgctt ggtagcggaa cgacgcttcg gcaatgtccc
     1081 gctggggaat gttcagcttg atcgcctcga ccatgccgcc tgcgtcctcg atccgctgga
     1141 agatctcgta cgcgccgcgc tcgagctcgt tcgtgaggtg ctcgacgtag taggagcccc
     1201 cgagcgggtc gatcgcgttc accacccccg tctcgtgagc gatcacctgc tgcgtgcgga
     1261 gcgcgatgcg aaccgcgtcc tcggtcggga gcgcgagagc ctcgtcgtac gagttcgtgt
     1321 gcaggctctg cgtgccgccg agcaccgccg cgagcgcctc gatcgccgtg cggacgatgt
     1381 tgacctcggg ctgctgcgcc gtcagtgaca cgccggccgt ctgcgcgtgg aaccgcatca
     1441 ggcaggattg ctcgttcgtt gcgccgtagc gttcgcgaag ctcgcgcgcc cagattcgac
     1501 gggccgcgcg gtacttcgcg atctcctcga agaagtcgag gtgcgcgttg aagaagaagc
     1561 tcagacgcgg ggcgaagtcg tcgatctcga gtccacgctc caacgcccac tcgacgtagg
     1621 tgaaaccgtc ctgcagcgtg aacgcgagct cctgcaccgc ggtcgagccc gcctcgcgga
     1681 tgtggtagcc ggagatcgag atcgggtgcc agagcggcat ttcccgggcg cagaactcga
     1741 tcatgtccgt cacgagccgc atcgagggct cgggcgggaa gatgtactcc ttctgcgcga
     1801 tgtactcctt gaggatgtcc gtctggatcg tcccccgcag cgccgcccgg tcggcgccct
     1861 gctgctcgcc gacgcagagg tagaaggcga gcagcatcgc ggcgggggcg ttgacggtca
     1921 tcgacggcga gacgtcggcg cgcgggatcc cgtcgaacag cgactccatg tccgccagcg
     1981 aatcgatcgc gacgccctct cggcccacct cgcccaccgc gcgggcgtgg tcggagtcgt
     2041 accccatcag ggtgggcatg tcgaaggccg tcgagagtcc ggtctgtccg tgctcgagga
     2101 ggtagtggaa ccgctcgttg gtctcctcgg ccgtgccgaa accggcgaac tggcgcatcg
     2161 tccacagccg cccgcggtac atggacgggt agacaccccg tgtgagaggg taggcccctg
     2221 gatagcccag gtcgcgctcg tagtcgatat cgaccgtctc cggcgtgtgc aggggctcgt
     2281 tctcgagccc cgagatggtc gtcgtcagct cgccctcgcg cggcggagtc gcgtcgaagc
     2341 gcttccgcca cgttcgcagc ccaccgaccg cgtcgcgatc ctcggtggtc gccattgcat
     2401 ggaagtctag gccagccagc cggccgccag ccccgcgccc ccaacgaggg atccgccccg
     2461 agaggcggcc cgccgatgcg gtaggcggtg acgttctccg cacgagcagc ggctctcgtc
     2521 gcggttcttg cagcgacggt ggcgaccggg gcgaccgctc atgccgcgtc gagctgcggc
     2581 ggcggctcgc atggcaagca ggactacggg tatggcggac accaggccgc gaagggcacg
     2641 agcgggatcc gcgccgccat caccgcaacg cggactccgt cggtggacga cggccatgtc
     2701 gccggctggg tcggagtcgg cggccccggg gccgggccgg gaggccgaga tcagtggctc
     2761 caagtcggtc tcgcggcgct ccccggctcg ggcgtcctgc tgtacacgga ggtgacccgc
     2821 gcgggccggg agccggtctt ccggctggtc gagctcgacg tgacacccgg tgcgacccgc
     2881 accgtcatcg tccgcgagac cgcccgccgg ggctggtggc aggcctccgt ggacggaagg
     2941 ccggtgacga agcccgtcca cctttcgggg atgcaaggtg tccggccgat tgtcacgacc
     3001 gaggcgtgga accccggctc acgctcctgt aaccgtttct cgttcaggtt cgacgacgtc
     3061 tccgtcgtcg caccgggggg cggctcctgg cggccgttcg tgcccggtct ccacttccgc
     3121 gactccggct tctccgtccg cgcggtgaag cctcgagcgg cgagcggaga ccgcgcgctc
     3181 gcggcgcaga accgggcgtc gttcgctttt ctcgcgtccg ccggttagcc cctgacgacc
     3241 ttgatcggcg tgccgaccga gacgcggctg cgcaagaaga gcgcgtcgcg gtcgtggagc
     3301 cgaacgcagc cgtgcgagac cgcctggccg agcaggtgcg gcgcgttcgt gccgtggagg
     3361 ccgacgactc cgccgcccgg ccactccgag agcttcgagt acgcgctcgt ctcgaaggcg
     3421 aaggcgccga ggaccgacca ggacggcttg aacttcgccg tcacgtagaa gaggccgagc
     3481 ggggtctcca tcccgcgtgc gcccacggcg acctttccgc ggcgcacgag ccggtcctcg
     3541 atccagaact cgaagcgccg cgtctcccga tagatgatca ccttgcgatc gacgggctcg
     3601 agctcgagct gcgccgcgcg gatccagccg gtgcgtccgt tgggtcggcc gggaagagcg
     3661 atccggtacc aggccggccc gcccgtgctc ggatggaggc gctcggcgac ggcgagcacc
     3721 acacgcggat cgaagctctt gcggaactgg gtgagcaccg ccacccgtcg cgccgtattc
     3781 gtggggcgcg cgcgcacgac gaccctgggc tgcgtcagcg tgcccgagcc gacaacctcg
     3841 acgcgggccg aaccggctgc gtccgaaggg cttgcgtcgc gtgcgagcac gacggccagc
     3901 agcgagatcc cgaacagagc gaagacaaac cgcgtcgtgg ccatcccact cacaacgtat
     3961 gactccaaag ggggagtcgg tatcgctcga tcgagtggtt acgtgcccct tccgggggag
     4021 agcgaaaaag agggggcggc cctgcaggac cgccccctcg atcgcgctgc tgtcaggccg
     4081 atctcctagc ccgtcaccgg cggctggaag atcccaacga cgccgatccg ctgggtgttg
     4141 cagcccttct tgttgcggac cgagacggtg aggattcctg cctttctcgg cttgagcgtg
     4201 acgacgatga cgccggtctt gccggtccgg acgatcttga agacgccggg gcccttgatc
     4261 cggaccgccg cccgtgcgac tggcttgccg cccgcggtca cccggacctt gaagcgctgc
     4321 ctctttcccg tggcccgcag gagcttcttg gtcaccttca cagtgctgca gatcgtcggc
     4381 ttgcgaaccg gcggcttcgg ctttgcctgc ggctgtttcg gcttcggctt cggcttcacg
     4441 gtcgacttcg gaggcgtcgc cggtgcgacg acgagcgtgg tcgtcgacgc cgtgttgtcg
     4501 ccggggttga cttctgcccc gcccgatcct gtgacggtcg ccgtgttggt cacgaggccc
     4561 gtcctctccg cgcgggcgat cacggtgact tcgatcgtct cacccttgcc cagactgccg
     4621 aggtcgcact ggacgagcgc agcgctgacg cgacaggtgc ccttgctcgg tgacgcgctg
     4681 aggaacgcaa taccggtggg cgccggatcc gccacctgga catcgatcgc ccggttcggg
     4741 ccgttgttca cgacgcggag ggtgtaggtg atcgtcttgc cgacctgcac cggatccggc
     4801 cgatcccact tggccacccc cacgtcgatc cgcggggtcg actggatcac cggaggcgga
     4861 ggcggcggag gcggaggcgg aggcggaggc ggaggcggag gcggaggcgg aggcggaggc
     4921 ggcggaggcg gcggaggcgg cggagg
//
LOCUS       DBNGNK010000224         3687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_613124,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000224 DBNGNK010000000
VERSION     DBNGNK010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_613124"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="AC150V_06595"
     CDS             <1..1041
                     /locus_tag="AC150V_06595"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016851 - magnesium chelatase activity
                     [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR3762627.1"
                     /translation="ERLKQALLLNAVNPPVGGVLIEGPSGTGKSTAVRGLAALLPEIP
                     VVSDCPFSCDPSSLCVRCEERRAAGEVLPAGRRPVRIVTLPLNATEDRVAGSIDIARA
                     LHEGVTALEPGLLAEVNRGILYVDEINLLDDHLCDILLDAAALGVNVVERESVSVSHP
                     ARFLLVGTMNPEEGELRPQLADRIGLRVEVDGLTSVDERAEIMRRREAFTDDPESFVG
                     EWSGDTAELAAAVERARAAVGDVKLQPEQYEAIAQLVVRSGVTSHRADVTILQCAKAL
                     AALAGRPTVERDDLLAASLLALSHRLERDPFEPGEGIDERILQRVLDDVLDVETAEKK
                     AAGPGTRAPSSI"
     gene            1164..1901
                     /locus_tag="AC150V_06600"
     CDS             1164..1901
                     /locus_tag="AC150V_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3762628.1"
                     /translation="MALRRGKHSRHRLAGSGDRDIALDATLRAAARRAGRHRTDVAIE
                     LEDLRRKVREHRVPLEVCIVVDNSYSLQADRMVEKAKGLAYGLLADAIGKRDRVSLVA
                     FKSGVPEATVALRPTSSVHVAATRLREVPLSGRTPLAHAVRLAGRLLRQEGAKRPNAK
                     PVLVVVTDGLPNVPRRPGGDPVADALLETRALRRRGVRLVVVDASLPGRKAQSCAAAL
                     AEAGGGPHLSIGDVSPGVFLDVLDRVA"
     gene            1911..2534
                     /locus_tag="AC150V_06605"
     CDS             1911..2534
                     /locus_tag="AC150V_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762629.1"
                     /translation="MLAPDQAAVLEAILDPRIAMDRGASPGIADMLHARGLLDPEARD
                     TARAALTEARRQRPVEWVTGTVTQGEVFEAFVRHCADELDDVTVVEAEADALVTRWRD
                     ETSRVELRAGFMGIERLVSDEPTMLIGVIGDDTRPLVDAFLDDPELRSKLAVCDLTRL
                     ERVGTPKASVFVYFEWFLREAYNVKLLPAPAFTQGLIERGIISLGMG"
     gene            2562..>3687
                     /gene="mnmA"
                     /locus_tag="AC150V_06610"
     CDS             2562..>3687
                     /gene="mnmA"
                     /locus_tag="AC150V_06610"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR3762630.1"
                     /translation="MPPVEVVGDSARGSEWAAVRLLVEGDTIVSADAPGMRRPLDGLT
                     LLEAAAVPGETLAADALANALARVFRAAPTPRRVAVAMSGGVDSAVALLRAAPDAIGV
                     TLRLWIDPSGPDTERACCSPSAVLAARRTCHELGLPHVTLDLRQAFRESVVKPFVDGY
                     AAGLTPNPCTRCNGSFRFHELIAFAQRAGAERLWTGHYARVVERGGRRLVARGVDAAK
                     DQSYMLATLPPALLERVHFPLGEQTKDETRSEARRAGLAAADRRESQEACFLGGADYR
                     SFLERHGVPRLAGPIVDENGAAVGTHEGAWRYTPGQRRGLGLASAEPLYALRVEGATN
                     TIVVGPRCSLACSGVRVRGELYVPVARAEAKLRYRSDPVGA"
BASE COUNT          524 a         1291 c         1330 g          542 t
ORIGIN      
        1 gagcgcctca agcaggcgct cctgctcaac gccgtcaacc cgcccgtcgg aggcgttctg
       61 atcgaaggtc cgagcggaac ggggaagtcc acggcggtgc gggggctggc ggcgctgctc
      121 ccggagatac cggtcgtctc cgactgcccg ttctcgtgcg acccgtcgtc cctgtgcgtg
      181 cgctgcgagg aacgccgggc agcgggcgag gttctcccgg cagggcgccg gccggttcgc
      241 atcgtcacgc tgcctctgaa cgccaccgaa gaccgggtcg ctggctcgat cgacatcgcg
      301 cgcgccctcc acgaaggcgt gaccgctctc gagccgggcc tgctggcgga ggtcaatcga
      361 ggcatcctct acgtcgacga gatcaatctg ctcgacgacc atctctgcga catccttctc
      421 gacgccgcag cgctgggggt caacgtcgtc gagcgtgaga gcgtctccgt gtcgcatccc
      481 gcgcgcttcc tgctcgtggg cacgatgaac cccgaggagg gcgagctccg gccgcagctg
      541 gccgaccgga tcgggcttcg cgtcgaggtc gacggactca cgagcgtcga cgagagggcc
      601 gagatcatgc ggcggcgcga ggccttcacg gacgatccgg agtcgttcgt cggcgagtgg
      661 agcggcgaca cggccgaact ggccgcggcc gtcgagcggg cacgggcggc ggtcggtgac
      721 gtcaagcttc aaccggagca gtacgaggca atcgcgcagc tcgttgtgcg ctctggagtg
      781 acgagccacc gcgccgacgt gacgatcttg cagtgcgcca aggcgctcgc ggcgctcgcc
      841 ggccggccga cggtggagcg ggacgatctg ctcgcagcct cgctgctcgc gctctcgcac
      901 cggctcgagc gcgatccctt cgagcccggg gaaggaatcg acgagcgcat cctccagcgc
      961 gtgctcgacg acgtcctcga cgtggagacg gccgaaaaaa aagcggcggg cccggggacg
     1021 cgggcaccga gctcgatctg accgagttcg aggggatccc aggcatcgcc gggctcgaag
     1081 tcgacgcccg gcgggcgttc gacggcccgt cccgccccga ccggatcgag cggggggcga
     1141 tcggccgccg cgaccgctcc ctcgtggccc tgcgacgcgg caagcactct cgccaccgcc
     1201 tggccggcag cggcgaccgc gacatcgcgc tggacgcgac gctccgcgcg gccgcgcgtc
     1261 gcgcgggtcg gcaccgcacg gatgtggcga tcgagctcga ggatctacgg cgcaaggttc
     1321 gcgagcatcg tgtcccactc gaggtctgca tcgtcgtcga caacagctac tcgctgcagg
     1381 ccgacaggat ggtagagaag gctaagggtc tggcctacgg gctcctcgcg gacgcgatcg
     1441 gcaagcgcga ccgcgtttct ctcgtcgcct tcaagtccgg cgtcccggaa gcgacagtcg
     1501 cgctccggcc gacgtcgagc gtgcacgtcg cggcgacgcg cctgcgcgag gtacccctct
     1561 cggggcgcac tccgctcgct cacgcagtcc ggctcgccgg tcgcttgttg cggcaggagg
     1621 gcgcgaagcg accgaacgcg aagccggtgc tcgtcgtcgt cacggacggc ctcccgaacg
     1681 tcccgcggcg acccggtggt gaccccgtcg cagacgcgct tctcgagaca cgcgccttgc
     1741 ggcgccgcgg tgtgcgtctc gttgtcgtgg atgcgagtct gccgggacgc aaggcgcaga
     1801 gctgcgccgc ggccctcgcg gaagcaggcg gaggcccaca tctctccatc ggcgacgtct
     1861 caccgggcgt cttcctggac gtcctcgacc gggtcgcgta gaacgtgggc gtgctcgcac
     1921 ccgatcaggc ggccgtcctg gaggcaatcc tcgacccccg gatcgcgatg gaccgtgggg
     1981 cgtcgcccgg gatcgcggac atgctgcacg cgcgagggtt gctcgatccc gaggcacgcg
     2041 acaccgctcg ggcggcgctg accgaggcgc gcaggcagcg tccggtcgag tgggtgacgg
     2101 gcacggtgac gcagggagag gtgttcgagg ccttcgtgcg ccactgcgcc gacgagctcg
     2161 acgacgtgac cgttgtcgaa gccgaggcag acgcgctggt gacacgctgg cgggacgaga
     2221 caagccgggt cgagctccgt gccggcttca tgggcatcga gcggctcgtg tccgacgagc
     2281 cgaccatgtt gatcggggtc atcggcgacg acacccggcc cctcgtcgac gcattcctcg
     2341 acgatccgga gcttcgctcg aagctcgccg tctgcgacct cacgcgactc gagcgtgtcg
     2401 gcacgccgaa ggcaagcgtg ttcgtctact tcgaatggtt cctccgagag gcgtacaacg
     2461 tgaagctcct gccggcgccg gctttcactc aaggattgat cgagcgcggc atcatctcgc
     2521 tgggcatggg ttagtcggcg gtggctagct agcctgggca cgtgcctccc gtcgaggtcg
     2581 ttggtgattc cgcccgaggc tccgagtggg ccgcggttcg gctgctggtc gaaggcgaca
     2641 cgatcgtctc tgccgacgcg cccggcatga ggcgccccct cgacgggctg acgctgctcg
     2701 aggcggccgc cgtcccgggc gagacgctcg ccgcggacgc cctcgcgaac gcgctggcgc
     2761 gggtgtttcg cgccgcgccc acgccacgcc gcgttgccgt cgcgatgagc ggaggcgtcg
     2821 acagcgccgt cgcgctcctg cgggctgcac cggacgcgat cggtgtcacc ctccgcctct
     2881 ggatcgaccc ttccgggccc gacacggagc gcgcctgctg ctcgccgagc gccgtgctgg
     2941 cggcgcgccg gacgtgccac gagctcggtc tgccgcatgt cacgctcgac ctccggcagg
     3001 cgtttcgcga gtccgttgtg aagccgttcg tcgacggcta cgcagcggga ctcacgccga
     3061 acccgtgcac gcggtgcaac gggagcttcc gctttcacga gctgatcgcc ttcgcgcagc
     3121 gggccggcgc cgagcggctc tggacaggcc actacgcgcg cgtcgtcgag cgcggcggac
     3181 gccgcctcgt ggcgcggggc gtcgacgcgg ccaaagacca gtcgtacatg ctcgcgacac
     3241 tccctccggc gcttctcgag cgagtgcact tcccgctcgg cgaacagacc aaggacgaga
     3301 cgcgctccga agcccgccgg gcgggtcttg cggcggccga tcgtagggag agccaggaag
     3361 cgtgctttct gggaggagcc gactaccgct cgttcctcga gcgtcacggc gttccacgcc
     3421 tcgccgggcc gatcgtcgac gagaacggcg cggcagtcgg gacgcacgaa ggtgcgtggc
     3481 gctacacacc ggggcagcgg cgcgggctcg gtctggcctc tgccgagccg ctctacgcgc
     3541 tccgcgtgga gggagccacg aacacgatcg tcgtcgggcc aaggtgctcg ctcgcgtgca
     3601 gcggcgtccg cgtgcgggga gagctctacg tcccggtcgc ccgcgcggaa gcgaagctac
     3661 gctaccgctc ggatccggtg ggagcga
//
LOCUS       DBNGNK010000225         6436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1545560,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000225 DBNGNK010000000
VERSION     DBNGNK010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1545560"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="AC150V_06615"
     CDS             complement(<1..1864)
                     /locus_tag="AC150V_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020440230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3762631.1"
                     /translation="MKRLGVDVGGTFTDLILVDEETGRITVDKVPSTPDDPARGSVAG
                     TRDLCEKAGVGLGEIDMLLHGTTVATNIALTHSGAEVGMITTEGMRDILHIARHKKPL
                     NFSLQQELPWQARPLVKRRNRLTVSERVTVPDGEIVTPLDEDEVRERVRQLRDAEVDA
                     VAVCFLHSYLNPEHEQRVKEIVLEEFPEAYLSVSNEVLPLYREFERFSTVALNAYVGP
                     KVSRYVSRFDEEMREAGFTHGVQLMQSSGGMATVESAAQRPVNLLMSGPVAGLIGGIW
                     AGKLAGYDNVVTLDIGGTSADIGVAAGGQLRMRHLLDTKIGDYQAMVPMVDIDTIGAG
                     GGSIAYVDQGGVFRVGPESAGADPGPACYGRGGTLPTATDAQLLLGRLRSDRGLLGGE
                     MRLDRDLAEKAMGDLATELDMSVEEAALGALQIQKFGMTQAIELNSVRRGYDPREFTL
                     VAAGGAGPLFACDIALELEIGRVLVPPHPGIIAATGLLATDLQHEFVATERHALKTLD
                     RERLSTRFDELVAQAVGRLEEDGVPEEQRLVRRLADCRYAGQGYEVRTEVPAGAVDEA
                     WVGELQERFHAAHEHEYGHRFDAAIEIINIRAVGIGRVEELQPTTLEPGTGDASG"
     gene            complement(1874..2884)
                     /locus_tag="AC150V_06620"
     CDS             complement(1874..2884)
                     /locus_tag="AC150V_06620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3762632.1"
                     /translation="MGTIGSQYMCQDFAECIASVQKAEEAGYSHAWFIDSQILWQDCF
                     VYMTSALAATDRIVVGTAVTNPYTRHVTTTASAFATLAELHPGRLELGIGRGDSAVRT
                     MGLNPVKTSFLQESVPVLRDLMAGRTVTINDSDVHFRWLDGDAEIPIMMSATGPKNLR
                     LAGSLADRVMLYVGVNETSLRWAIDHVRAGAEEAGRDPDAIRFSVLTAMWVSDDQEEA
                     WDKCRWAPAACANHIADTMKRNPSHGMPETMTRLPQSRDDYDYYAGHLSSDADHTAYL
                     TGELIDDFTLAGPAEKVRDGVRALFDLGIDEISCAYLNGAFDQMDTVGREIIPATVTT
                     KR"
     gene            complement(2902..3552)
                     /locus_tag="AC150V_06625"
     CDS             complement(2902..3552)
                     /locus_tag="AC150V_06625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR3762633.1"
                     /translation="MSEMHYYDPAAWRLEPGRTVHVVIDDQNDFLHPDGWYATHDIDI
                     SHMRRVIEPTKRLNEECRRRCVPILWTRHGTRGVEDGGPFMRKRLLLLEGGLRKDTWG
                     FEILADLEPGPDDWYVEKTRLSAFFQTNLEQILRALGAETVILSGVLTNQCIGATCKD
                     ALFRDFKPIVVEDCVGTATPHLHDPAIEMIRVGWGQVNTLEQTLEELSAFPSAVAV"
     gene            3645..4292
                     /locus_tag="AC150V_06630"
     CDS             3645..4292
                     /locus_tag="AC150V_06630"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3762634.1"
                     /translation="MKAPYLRDQAFARVRDAIVSGALEPAAPLVIDDLAATFGLSAMP
                     VREAVKRLVGDGLVEELPRRAHRVAPLTRMTALNVLDVMETLMVRAYELGLPALQPSD
                     VAAMQAALDAAHARAASGDLIAALASIHEMHRVVYQATGNPEFERALGSIGPRFDRVL
                     YLWYTESIVEAGSSYRRELVDALLRDDRAGALEIMRHAWARFRDVVAARKDETAE"
     gene            4289..>6436
                     /locus_tag="AC150V_06635"
     CDS             4289..>6436
                     /locus_tag="AC150V_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR3762635.1"
                     /translation="MIDRARVVVVGGGIGGVSTLYHLTKLGWNDVALIEAHELTSGST
                     WHAAGLCTQFNQSYNLMGLLTYSLDLYETLEAETGQAVDLHRCGSVRIGTTTDRLHQF
                     EHVCGIAASVGVPVEIITPERAAELFPLAQPDGVLAAAYLPTDGHVDPTGLTNALAKG
                     ATDRGAAIVRHTPVTALARDGAVWTVSTAAGEVRADHVVIAAGQWSRQVARLAGVELP
                     IIPLQHHYVLTEPIAEIESRTTELPVFRDPDNSFYARQEGRGLLVGPFEPNPKTWALD
                     GVPDDFHGRLLPPDLDQIEGCLVAAAGRIPVFGDVGLATVINGPDGYTPDGKCLMGPV
                     PGQPGLHVLAGFSIFGIVFGGGAGRYAAEWIVEGQPSDNMWELDVRRYGDYANATSYV
                     VDKASEVFEDEYKIHYPEEERPAGRPLKTDPLYQRLLGKGAVMGVRSGWERPLWFARN
                     GVARDEYSFRRGNWFEAVGEECRAVRGGVGVLDQTSFAKFIVSGEGARALLDRICANR
                     LPTEAGRLSLTQMLTSRGGIEADVTVALVEPDRFYVVSAAATETHDLAWIQRHAPADG
                     TVEIENATDRFGVLTIAGPRSRELLQRLTDDDLSNEGFPFFRARHIELARERVLAVRI
                     SYVGELGWEIHHPLRHQTALYESLFDAGADLGLVDFGYSALESMRFEKCYRLWGADLS
                     ADWSPLQAGLGRFVAFDKGDFIGREALLRERAQG"
BASE COUNT          942 a         2207 c         2223 g         1064 t
ORIGIN      
        1 cgccggaggc atcacccgtg ccgggctcga gcgtcgtcgg ctgcagctcc tcgactcggc
       61 cgatgccgac ggcgcggatg ttgatgatct cgatcgcggc gtcgaagcgg tgcccgtact
      121 cgtgctcgtg cgcggcgtgg aatcgctcct ggagctctcc cacccaggct tcgtcgaccg
      181 ctcccgccgg cacctcggtg cgcacctcgt agccctggcc ggcatagcgg cagtcggcca
      241 gccggcggac gagacgctgc tcctcgggga caccgtcctc ctcgagccgt cccaccgcct
      301 gggcgacgag ctcgtcgaat cgtgtggaca gtcgctcgcg gtcgagggtc ttgagcgcgt
      361 ggcgctcagt ggcgacgaac tcgtgctgga ggtcggtcgc cagcaggccg gtggcggcga
      421 tgatgccagg gtgcggcggc accagcacgc ggccgatctc cagctcgagc gcgatgtcgc
      481 acgcgaacaa cggcccggcg ccgcctgcgg cgacgagcgt gaactcgcgc gggtcgtagc
      541 cgcggcgcac cgagttgagc tcgatcgcct gcgtcattcc gaacttctgg atctggagcg
      601 caccgagcgc ggcctcctcg accgacatgt cgagctcggt cgccagatcg cccatcgctt
      661 tctcggcgag gtcgcgatcg agcctcatct cgccgcccag cagaccacgg tctgagcgga
      721 ggcgaccgag caggagctgg gcgtccgtcg ccgtggggag cgtgccgcct cgcccgtagc
      781 acgcggggcc cgggtccgca ccggccgact cggggccgac acggaacacg ccgccctggt
      841 cgacgtaggc gatcgagccg cctccggcgc cgatcgtgtc gatgtcgacc atcggcacca
      901 tggcctggta gtcgccgatc ttcgtgtcga gcaggtggcg catccggagt tggccgccgg
      961 cggcgacgcc gatgtcggcc gacgtgcccc cgatgtcgag cgtgacgacg ttgtcgtagc
     1021 cggccagctt gccggcccag atgccgccga taagcccggc gaccggacct gacatgagga
     1081 ggttgaccgg ccgctgcgcc gccgactcca cggtcgccat gccgccggac gactgcatca
     1141 gctgcacgcc gtgcgtgaag cccgcttccc gcatctcctc gtcgaaccgg gagacgtacc
     1201 gcgacacctt tggaccgacg taggcgttga gggcgacggt cgagaagcgc tcgaactcgc
     1261 ggtagagggg tagcacctcg ttcgagacgg agaggtatgc ctcggggaac tcctcgagca
     1321 cgatctcctt cacacgctgc tcgtgctctg ggttgaggta gctgtgcagg aaacagacgg
     1381 cgacggcgtc gacctcggcg tccctaagct ggcgcacgcg ctcgcgcacc tcgtcctcgt
     1441 cgagcggcgt cacgatctct ccgtcgggga cggtgacgcg ctcggagacc gtgagccggt
     1501 tgcggcgctt gacgagcggg cgcgcctgcc acggcagctc ctgctggagc gagaagttga
     1561 gcggcttctt gtgccgggcg atgtgcagga tgtcacgcat cccctcggtg gtgatcattc
     1621 ccacctcggc gccgctgtgc gtgagcgcga tgttggtcgc aaccgtcgtc ccgtgcagga
     1681 gcatgtcgat ctcgcccagg ccgacgcccg ctttctcgca caggtcgcgg gtgccggcga
     1741 cgctgcctcg tgcggggtcg tcgggagtgg aggggacttt gtcgaccgtg attcggccgg
     1801 tttcctcgtc tacgaggatc aggtcggtga acgtgccgcc gacgtcgacg ccgagccgct
     1861 tcatcatcgc ctcctaccgc ttcgtggtga ccgtcgccgg gatgatctcg cgcccgaccg
     1921 tgtccatctg gtcgaacgcc ccgttcaggt aggcgcacga gatctcgtcg atgccgaggt
     1981 cgaagagcgc gcgcacgccg tcgcgtacct tctccgcggg cccggcgagc gtgaagtcgt
     2041 cgatcagctc gccggtgaga tatgcggtgt ggtcggcatc ggaggagagg tgaccggcgt
     2101 agtagtcgta gtcgtcacgg ctctgcggca gcctcgtcat cgtctcgggc attccgtggg
     2161 acgggttgcg cttcatcgtg tcggcgatgt ggttcgcgca cgcggccggg gcccagcggc
     2221 acttgtccca agcctcctcc tggtcatcgc tgacccacat cgcggtgaga accgagaatc
     2281 ggatcgcatc gggatcgcgg ccggcctcct cggcgccggc gcggacgtga tcgatcgccc
     2341 agcggagaga cgtctcgttg acgcccacgt agagcatgac ccggtctgcc agcgagccgg
     2401 ccagccggag gttcttcggg ccggtcgccg acatcatgat cggaatctcg gcgtcgccgt
     2461 cgagccagcg gaagtgcacg tccgagtcgt tgatggtgac cgtccggccc gccatgagat
     2521 cacggagcac cggaacggac tcctgcagga acgaggtctt gaccgggttc aggcccatcg
     2581 ttcggacggc gctgtcaccg cggccgatgc cgagctccag ccgcccgggg tgcagttccg
     2641 cgagcgtggc gaaggcgctt gcggtcgtgg tgacgtgccg cgtgtacggg tttgtcaccg
     2701 cggtgccgac gacgatccgg tccgtcgcgg cgagggcgga ggtcatgtag acgaagcagt
     2761 cctgccagag gatctgcgag tcgatgaacc aggcgtgact gtagccggcc tcctcggcct
     2821 tctgcaccga ggcgatgcac tcggcgaagt cctggcacat gtactgggag ccgatcgtgc
     2881 ccacgtcttt ctcccttcct ctcagaccgc gacggcagag ggaaaggcgc tgagctcttc
     2941 gagcgtctgc tcgagcgtgt tcacctgtcc ccagccgact cgaatcatct cgattgccgg
     3001 gtcgtggagg tgcggcgtgg ccgtgccgac gcagtcctcg accacgatgg gcttgaagtc
     3061 acggaagagc gcgtccttgc acgtggcgcc gatgcactgg ttggtcagca caccgctcag
     3121 gatcacggtc tcggcgccga gcgcccgcag gatctgctcg aggttcgtct ggaagaaggc
     3181 ggagagccga gtcttctcga cgtaccagtc gtcaggcccg ggttccaggt cggcgaggat
     3241 ctcgaagccc cacgtgtcct tgcggagacc gccttcgagc agcagcagac gcttgcgcat
     3301 gaaggggccg ccgtcctcga cgccacgtgt cccgtgccgc gtccagagga tcggaacgca
     3361 gcgccgccgg cactcctcgt tcagccgctt ggtcggctcg atcacacgcc gcatgtggct
     3421 gatgtcgatg tcgtgcgtgg cgtaccagcc gtccggatgc aggaagtcgt tctggtcgtc
     3481 gatgacgacg tgcaccgtgc gtccgggctc gagtcgccac gcggccgggt cgtagtagtg
     3541 catctcggac atccaacgcc tcctctaccg aggtttcgga tctcagatat gaagactcta
     3601 tactgcccgc catcgagtct caagccgtca ccccgctgag cccgataaag gctccgtacc
     3661 ttcgcgacca ggcgttcgcg cgtgtccgcg acgcgatcgt gtcgggcgcc ctcgagccgg
     3721 ccgctcctct cgtgatcgac gacctcgccg ccacgttcgg gctgagcgcg atgccggtgc
     3781 gggaggctgt gaagcggctc gtgggtgacg gtctcgtcga agagctgccg cggcgcgcgc
     3841 atcgcgtcgc tccgctgaca cggatgacgg ccctcaacgt gctcgacgtg atggagacgc
     3901 tgatggtgcg cgcgtacgag ctcggcctcc ccgctctcca gccgagcgac gtcgctgcga
     3961 tgcaagcggc gctcgacgcc gcgcacgcgc gcgccgcgag cggcgacctg atcgctgcgc
     4021 tcgcctcgat ccacgagatg caccgcgtcg tgtaccaggc aaccgggaac ccggagttcg
     4081 aacgtgcgct cggctcgatc ggtccgcgct tcgaccgcgt cctctacctc tggtacacgg
     4141 agtcgatcgt cgaggccggc agctcgtacc gacgcgagct cgtcgatgcg cttctccgcg
     4201 acgatcgcgc cggcgcgctg gagatcatgc ggcacgcctg ggcccgcttt cgtgatgtcg
     4261 tcgccgcccg gaaggacgag acggcagagt gatcgaccga gcgcgggtcg tcgtcgtcgg
     4321 aggcggaatc ggcggggtct cgaccctcta ccacctcacg aagctcggct ggaacgacgt
     4381 cgcgctgatc gaggcgcacg agctgacaag cggctccacg tggcatgccg ccgggctctg
     4441 cacccagttc aaccagagct acaacctgat ggggctgctc acgtactcgc tggacctcta
     4501 cgagacgctg gaggccgaaa ccggccaggc ggtcgacctg caccgctgcg gcagcgtccg
     4561 catcggcact acgaccgacc ggctgcacca gttcgagcac gtgtgcggca tcgccgcgag
     4621 cgttggcgtg ccggtcgaga tcatcactcc cgagcgggcc gccgagctgt ttccactcgc
     4681 gcaaccggac ggcgtcctcg cggccgcgta ccttccgacc gacggccacg tcgacccgac
     4741 cgggctcacg aatgcgctgg cgaagggcgc gaccgaccgc ggcgcggcga tcgttcgcca
     4801 cacgccggtc acggccctcg cgcgggacgg cgccgtctgg acggtttcca cggccgccgg
     4861 tgaggtgcgg gccgaccacg tcgtcatagc ggcgggccag tggtcgcgcc aggtggcccg
     4921 cctcgccggc gtcgagttgc cgatcatccc gctccagcac cactacgtgc tcaccgagcc
     4981 gatcgccgag atcgagtccc gcacgactga gctacccgtc ttccgtgatc ccgacaactc
     5041 cttctacgcc cgccaggagg gccgcggcct gctcgtcggc cccttcgagc ccaacccgaa
     5101 gacctgggcg ctggacggag ttcccgatga cttccacggc cggctgctcc cgcccgacct
     5161 cgaccagatc gagggctgcc tcgtcgccgc ggccggccgc ataccggtct tcggcgacgt
     5221 cgggctcgcg accgtgatca acgggcccga cggctacacg ccggacggga agtgcctgat
     5281 ggggccggta ccggggcagc cggggctcca cgtcctcgcc ggcttctcga tcttcggcat
     5341 cgtcttcggc ggcggggcgg gtcgctacgc ggcggagtgg atcgtcgagg ggcagcccag
     5401 cgacaacatg tgggagctgg acgtccgccg atacggcgac tacgcgaacg cgacgtcgta
     5461 cgtcgtcgac aaggcgtcgg aggtgttcga ggacgagtac aagattcact atcccgagga
     5521 ggagcggccg gccggccggc cgctgaagac agacccgctg taccagcggc tgctcgggaa
     5581 gggcgccgtg atgggcgtcc gctcgggctg ggagcggccg ctctggtttg cgcggaacgg
     5641 cgttgcccgc gacgagtact cgttccgacg cggcaactgg ttcgaggccg tgggggagga
     5701 gtgccgtgcc gtccgcggcg gcgtgggcgt gctcgaccag acgagcttcg cgaagttcat
     5761 cgtctcgggg gaaggggctc gggcgctact cgaccggatc tgcgcgaacc ggcttccgac
     5821 cgaggcgggc cggctctcgc tgacgcagat gctcacgtcc aggggcggga tcgaggccga
     5881 cgtgaccgtc gctctcgtcg agcccgaccg cttctatgtc gtctcggcgg cggcgacgga
     5941 gacgcatgac ctcgcttgga tccagcgtca cgcaccggcg gacggcacgg tcgagatcga
     6001 gaacgcaacc gaccgcttcg gcgtgctcac gatcgcaggc ccccgctcgc gcgagctgct
     6061 gcaacgcctg accgacgacg atctctcgaa cgagggtttt cccttcttcc gggcccgcca
     6121 catcgagctc gccagagaac gtgtgcttgc cgtccgcatc tcgtacgtcg gggagctcgg
     6181 ctgggagatc caccacccgc tccggcatca aaccgcgttg tacgagtccc tcttcgacgc
     6241 cggagccgat ctgggcctcg tcgacttcgg ctacagcgcg ctcgagtcga tgcgcttcga
     6301 gaagtgctac cggctctggg gcgccgacct gtccgccgac tggtcgccgc tccaggccgg
     6361 cctcgggcgc tttgtggcct tcgacaaggg cgacttcatc ggccgcgaag cgctccttcg
     6421 tgagcgtgcg cagggg
//
LOCUS       DBNGNK010000226         8080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_756776,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000226 DBNGNK010000000
VERSION     DBNGNK010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_756776"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC150V_06640"
     CDS             complement(<1..240)
                     /locus_tag="AC150V_06640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762636.1"
                     /translation="MPDRDRHTLGLFAPLGSTYDRVGAVLSFGQDPRWRRFLVERIPP
                     EPAHVLDVATGTGLVATELLAHGHRVTALDRSPEML"
     gene            261..818
                     /locus_tag="AC150V_06645"
     CDS             261..818
                     /locus_tag="AC150V_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762637.1"
                     /translation="MGLVEDLERTAEQAAALAAEGEKVAAVLAAEPTPGARGYLCAFE
                     NAAAERTWLALDAAGKPLDDRREIRDLVSIAALCEIAEENAGGGDLAALRSRLESLRG
                     GGRDEEVDEAVAALDALQVELGSPPQLATPGRLDSIGQATRRLERALDPTVGSPFAEA
                     MRAAQGAVEELRGEVESTYRVELNG"
     gene            828..1118
                     /locus_tag="AC150V_06650"
     CDS             828..1118
                     /locus_tag="AC150V_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762638.1"
                     /translation="MEGGFGIPFGGDPEEIMRGLREFAERQAESVQDAQREQFATLTL
                     NTAVELTSAALQRIDVKGSSDEQAIALRDAMRVLFPEAVALVSAARQGFMRE"
     gene            complement(1141..2046)
                     /gene="arcC"
                     /locus_tag="AC150V_06655"
     CDS             complement(1141..2046)
                     /gene="arcC"
                     /locus_tag="AC150V_06655"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR3762639.1"
                     /translation="MDRLVVALGGNALLRRGEPLEADVQRDNVHHAALALAELAESHE
                     LVVTHGNGPQVGLLALQSFACESIEPYPLDVLGAESEGMIGYLLEQELGNALAGRSTA
                     ALLTQVVVDADDRAFTHPTKPIGPLYSEDAARSLAAARGWTIAPDTGGWRRVVASPEP
                     IAIVELATIRLLVEHGVVTVCAGGGGIPVRSDEVGALHGVEAVIDKDLSAALLARSLG
                     ADLLLLLTDVHAVERGFGTAAAEKIRRATPAELRCEEFAPGSMGPKVEAACRFVEATR
                     RRAVIGSLADARRLVDGAAGTLVTF"
     gene            complement(2046..2954)
                     /gene="argF"
                     /locus_tag="AC150V_06660"
     CDS             complement(2046..2954)
                     /gene="argF"
                     /locus_tag="AC150V_06660"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR3762640.1"
                     /translation="MSARAAPVPDHFLRVTDLDADGLLALLDLAAEMKQRPHGFVEAL
                     RGETLVCFFEHPSTRTRVSFAAAAERLGMLPLLIQPEELQLGRGETMADTARALSGYA
                     AAIAIRARAQGTVDELATAAKVPVINALTDQNHPCQALADVLTLRERFGRLDGLRVAY
                     VGDGNNVANSLVEAGALLGIEVVVAAPAGYEPTTPGAVVVNDPAEAARGAHAVYTDAW
                     VSLGDEAERGSRLRELAPYQVDSPLLRHAKPDAVFMHCLPAHRGQEVTADVIDGPQSI
                     VWQQAENRLPTEEALVYALVLRTWSR"
     gene            complement(2983..4326)
                     /locus_tag="AC150V_06665"
     CDS             complement(2983..4326)
                     /locus_tag="AC150V_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008084894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HIR3762641.1"
                     /translation="MRKVVIMGAGGRDFHDFNVVFRENADARVVAFTATQIPGIADRV
                     YPASLAGDHYPDGIPIRPESELADIIRSEQADEVVLAYSDLSHREVMHAASVALAAGA
                     DFRLLGPHATMLEARKPVIAVTAVRTGSGKSQTSRKLGTILLGSGLRVALVRHPMLYG
                     DLEHMRVQRFASLRDIDAADPTVEEREEYEGPVGLGMTVFAGVDYAAVIEQAEAEADI
                     IIWDGGNNDFPFVRPDLLITVTDPLRAGDELAYHPGEANLRMADVVVVNKIDSADPWD
                     VDRVLADVQEVNPRAQIIRAESPVSLEAGPPVEGKRVLAVEDGPTLTHGGMSFGAGSV
                     AARAAGASELVDPRPWAVGSIAETFRLYPQIGPVLPAMGYSGAQLEELARTIEAAQCD
                     VVVTGTPIDLARLIETRHPVRRARYELREIGRPTLHDVIAPLERLAHVELAVSTG"
     gene            4427..5335
                     /locus_tag="AC150V_06670"
     CDS             4427..5335
                     /locus_tag="AC150V_06670"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR3762642.1"
                     /translation="MGLFDRAVVRMLPAVPRRVVRRVAAPYIAGPDLEDARHTVQSLN
                     GTGKRATIDVLGEEVTRPAEAEAIATAYREVLAAIDGHGLDASVSVKLTALGLKVDAS
                     LCGELLETVVDDAAQRGVFVRIDMEDSSCTDDTLVLYRRLREKGRDGVGVVFQSALRR
                     TLADIADLSDLRPNVRLCKGIYVEPPSVAFRDGDVIRRSFVAALDALVAGGSYVGIAT
                     HDEWLIQEALTRARALDESASEFQMLLGVRENRASELVADGHPLRVYVPFGRRWYEYS
                     LRRLQENPAVAGHVAAGVLRRTLPKL"
     gene            complement(5332..7122)
                     /locus_tag="AC150V_06675"
     CDS             complement(5332..7122)
                     /locus_tag="AC150V_06675"
                     /inference="COORDINATES: protein motif:HMM:NF013360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HIR3762643.1"
                     /translation="MAAGPAPGVEPGDNLPDLVSDPPENPRLQTYWDATGGRLLLRFD
                     GFVHNRGPGPLEIWGRSPVNLEMTDTFQRIFQTGGSSRDDASKSPWLIYETTDGHNHW
                     HFRSAASDMLYDSTTSAVVAPGQKVGFCLIDSEKVDAVGPPSRVYDAPSFCQRLQTQA
                     PEVMMGISAGWRDRYPWTLAFQWVDVSDVQPGTYWLASQADPDDTVVEADESNPVAFA
                     DTPSVLPGYVARSRSVQASGTTQIQLAADSYGSPGPIRYEIVGAPQHGTLDQGVGNPF
                     PLPTVTYTPASGFSGSDSFSYVAYDSSSGFPRSPAVATVSLSVSSIDPAVAISGAPAS
                     MLAGTSVQLAATVLNDPPNVTWSVNGVQGGNASTGTISSSGLYTAPPQPPTPDSVTVR
                     AASVGAYDEVSIRIEAQSTQPPAPTPNPPPPPPPASPPPPAAPPPPPASPAPPPAASP
                     PPPAAPAAVEDGQATAGDATPAEGTKAKKAKRRNLLQRPFLAARKRSLLISSRSARAG
                     KIEIKVLRGSSTIGRCTVRTPAKRDLTCRVMLKPGTKIKGMRVILKLRSGGKVVAYRR
                     ATFRKATQYYLGAALQCWIGPGAAKPKALG"
     gene            complement(7301..8035)
                     /locus_tag="AC150V_06680"
     CDS             complement(7301..8035)
                     /locus_tag="AC150V_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3762644.1"
                     /translation="MSFAGKSVLVTGGSRGIGKEICLRFAREGAARIAIGYMRSDSAA
                     EETAAEIRDLGADPVLVRGNVASERIASEVAELGRLDVFIHNAATGVIRPALETEDKH
                     WDWTHGTNARALLSLTRAAAPSMPAGSSIVALSSLGSVRVLENYALVGTSKAALEALV
                     RYLAIELGPSGIRVNAVSAGVVETGALEYFPNREAMLDMGRDNAAGRIVTPADIAGAV
                     LFLCSPDAEMVRGQVLVVDGGFSISA"
BASE COUNT         1178 a         2711 c         2950 g         1241 t
ORIGIN      
        1 gagcatctcg gggctgcgat ccagggctgt cacgcgatga ccgtgcgcga gcagctcggt
       61 ggcgacgagc cctgtgccgg tcgcgacgtc gagcacgtga gcgggttcgg gtgggatccg
      121 ctcgacgagg aaccgccgcc aacgcgggtc ctgcccgaac gacagaaccg caccgacacg
      181 gtcgtacgtc gagccgagcg gagcgaagag gccgagcgtg tggcggtcgc ggtcgggcac
      241 gtcgctctat tctcgctcgg gtgggcctgg tcgaagacct cgagcggacg gccgagcagg
      301 cggcggcgct ggcggccgag ggcgaaaagg tcgcggcggt gctcgctgcg gagccgaccc
      361 cgggtgcgcg cggctacctc tgcgcgttcg agaacgcggc ggcggaacgc acgtggctcg
      421 cgctcgacgc ggcggggaag ccgctggacg acagacggga gatccgcgat ctcgtctcca
      481 tcgcggcgtt gtgtgagatc gccgaggaga atgccggcgg cggcgacctc gccgctctcc
      541 gctcacggtt ggagtcactg cgcggcggcg gccgtgacga ggaggtggac gaggcagtgg
      601 ccgcgctcga tgcgctgcag gtcgagctcg gatcgccgcc acagctcgcg acgccgggcc
      661 gcctcgattc gatcggtcag gccacccgcc ggctggagcg tgcgctcgac ccgaccgtcg
      721 gctcgccgtt cgcggaagcg atgcgagcgg cgcagggtgc agtggaggag ctcagggggg
      781 aggtcgagtc gacctatcgg gtcgagctca acgggtaggc tggcggcatg gaaggcggct
      841 tcggcatccc cttcggaggc gatccggaag agatcatgcg cgggttgcgc gagttcgcgg
      901 agcggcaggc cgagtcggtg caggacgcgc agcgcgagca gttcgcgacg ctcaccctga
      961 acacagcggt cgagctgacc tccgccgcgc tgcagcggat cgacgtcaag ggcagctcgg
     1021 acgagcaggc gatcgccctc cgcgacgcca tgcgggtgtt gttccccgag gcggtcgcgc
     1081 tcgtatcggc cgcccggcag ggcttcatgc gggagtagcg gccgcgaggc agcggtgccg
     1141 tcagaaagtg acgagcgtcc cggccgcgcc gtcgaccagg cgtcgcgcgt ccgcgagcga
     1201 gccgatcacc gcgcgcctgc gggtggcttc cacgaaccga caggccgcct cgaccttcgg
     1261 ccccatcgag ccgggggcaa actcctcgca gcggagctcc gccggcgtcg cccgccggat
     1321 cttctctgcc gccgccgtcc cgaaacctcg ctcgaccgcg tggacgtcgg tgagcagaag
     1381 cagcagatcg gcgccgagcg accgcgcaag cagggcggcg gagagatcct tgtcgatcac
     1441 cgcctcgacg ccgtgcagcg ctccgacttc gtcgctgcgc accgggatgc cgcccccgcc
     1501 ggcgcagacc gtgacgacgc cgtgctcgac gaggagacga atcgttgcaa gctcgacgat
     1561 cgcgatcggc tccggcgacg cgacgacccg gcgccagccg cctgtgtccg gcgcgatcgt
     1621 ccacccccgt gcggcggcca gggaacgagc ggcgtcctcg ctgtagagcg gcccgatcgg
     1681 cttcgtcggg tgcgtgaacg ctcgatcgtc ggcgtcgacg acgacctggg tgaggagtgc
     1741 ggctgtgctg cggccggcca gcgcgttgcc gagctcctgc tcaaggagat acccgatcat
     1801 cccttcactc tcggcgccga ggacgtcgag cgggtacggc tcgatgctct cgcaggcgaa
     1861 gctctgcagc gcaagcaggc cgacctgtgg cccgtttccg tgcgtgacca ccagttcgtg
     1921 ggactcggcc agctccgcga gcgcgagcgc ggcatgatgg acgttgtctc gctggacgtc
     1981 cgcttcgagc ggctcgccgc gacggagcag ggcgttgccg ccgagggcca cgacgagtct
     2041 gtccatcaac ggctccaggt gcgcaggacg agcgcgtaga cgagcgcttc ctcggtcgga
     2101 agccggttct ccgcttgctg ccagacgatc gactgcggcc cgtcgatcac gtcggccgtg
     2161 acctcctggc ctcggtgggc gggaagacag tgcatgaaga ccgcgtccgg cttcgcatgt
     2221 cggagaagcg gggagtcgac ctggtacggg gccagctcgc gaaggcgcga tccgcgctcg
     2281 gcttcgtcgc ccagcgacac ccaggcgtcc gtgtagacgg cgtgggcgcc gcgcgcggct
     2341 tctgccgggt cgttcacgac caccgcgccc ggcgtggtcg gctcgtagcc ggccggcgcc
     2401 gcgacgacga cctcgatccc gagcagcgct ccggcctcga cgagcgagtt ggccacgttg
     2461 ttcccgtcgc cgacgtaggc cacccggagt ccgtcgagcc ggccgaaccg ctcgcgcagc
     2521 gtgaggacgt cggcgagcgc ctggcaggga tggttttggt cggtcagggc gttgatcacc
     2581 ggtacctttg cggcggtcgc gagctcgtcg acggttccct gagcccgcgc tctgatcgcg
     2641 attgccgccg cgtaacccga gagcgcccgc gcagtgtccg ccatcgtctc gccgcggccg
     2701 agctggagct cctccggctg gatcagcagc ggcagcattc cgagccgctc ggcggcggcc
     2761 gcgaacgata cgcgcgttcg ggtggacggg tgctcgaaga agcagacgag cgtctcgcca
     2821 cggagagcct cgacgaagcc gtgcggccgc tgcttcatct cggcggcgag gtcgagcagc
     2881 gcgagcagac catccgcgtc gaggtccgtg acgcgaagaa agtgatcagg gacgggtgca
     2941 gcccgcgcgc tcacttcgtc gtcactcgct ctcgtggcct cttcaacccg tcgacaccgc
     3001 gagctctacg tgggccaggc gctcgagcgg cgcgatgacg tcgtgcaggg tcggccgtcc
     3061 gatctcgcgc agctcgtagc gcgcgcgccg aaccgggtgc cgcgtctcga tcaggcgggc
     3121 gaggtcgatc ggagtccccg tgacgaccac gtcgcactgg gccgcctcga tcgtccgggc
     3181 gagctcctcg agttgggctc cgctgtagcc catggccggg agaacaggac ctatctgcgg
     3241 gtacagccgg aaggtctcgg cgatcgatcc gacggcccag ggtcgcgggt cgacgagctc
     3301 ggacgctccg gccgcgcggg cggcgacgct cccggcaccg aaggacatgc cgccgtgtgt
     3361 caacgtcggc ccgtcctcca cggccagcac ccgcttgccc tcaacaggtg ggcccgcttc
     3421 gagcgagacg ggcgactcgg cgcggatgat ctgcgcgcgc gggttgacct cctgcacgtc
     3481 ggcgagcacc cggtcgacgt cccatggatc ggcgctgtcg atcttgttga ccaccacgac
     3541 gtctgccatc cgaaggttcg cctcgccggg gtggtatgcg agctcgtccc cggcgcgaag
     3601 cggatcggtg accgtgatca ggaggtcggg gcgcacgaac gggaagtcgt tgttcccgcc
     3661 gtcccagatg atgatgtcgg cctccgcttc ggcctgctcg atcaccgccg cgtagtcgac
     3721 gcccgcgaac acggtcattc ccaggccgac gggcccctcg tactcctctc gctcctcgac
     3781 ggtcgggtcg gccgcgtcga tgtcgcgaag cgacgcgaag cgctggacgc gcatgtgctc
     3841 gaggtctccg tacagcatcg ggtgccggac gagcgcgacc cgcagcccag agccgagcag
     3901 gatcgtgccg agtttgcggc tcgtctggct ctttccgctt cctgtccgga cggccgtgac
     3961 ggcgatcacc ggcttccgag cttcgagcat ggtcgcgtgc ggaccgagca ggcggaagtc
     4021 ggcgccggct gcgagcgcga cggaggccgc gtgcatcacc tcccggtggg aaaggtcaga
     4081 gtacgccagt acgacctcgt cggcctgctc gcttcggatg atgtctgcca gctcgctctc
     4141 gggccggatc ggtatgccgt cgggataatg atcgccggcg agggacgcgg ggtagacgcg
     4201 gtcggcgatc cctgggatct gcgtcgccgt aaaggcgacg actctcgcat ccgcgttctc
     4261 ccggaacacg acgttgaaat cgtggaagtc gcggcctccg gcacccatga tcacgacctt
     4321 gcgcatgagc cctcctgggc atgattcgag cggctggagg ggccgcccgc atcggcgatg
     4381 ctgccgatcg cgtcgtggcg aactacggat gtacgatcgg cgcgtcgtgg ggctcttcga
     4441 ccgtgccgtc gtccgcatgc tcccggccgt cccgcggcga gtcgtccgtc gggtggccgc
     4501 gccgtacatc gcggggcccg acctcgagga cgcacggcac accgtgcaat cgctgaacgg
     4561 tacgggaaag cgggcgacaa tcgacgtgct cggggaggag gtgacgcggc ccgcggaagc
     4621 cgaggcgatc gcaaccgcct atcgggaagt gctcgcggcg atcgacggac atgggctcga
     4681 cgcgagcgtg agcgtcaagc tgaccgccct tggcctcaag gtcgacgcgt cgctgtgcgg
     4741 cgagctgctc gagacggttg tcgacgatgc cgcgcagcgc ggcgtcttcg tgcgcatcga
     4801 catggaggac agctcatgca cggacgacac gctggtcctg taccggaggc tgcgagagaa
     4861 aggccgggac ggagtcggcg ttgtcttcca gtcggcgttg cgtcggacgc tggcggacat
     4921 cgccgatctg tccgaccttc gccccaacgt ccgactgtgc aagggcatct acgtcgagcc
     4981 tccgtcggtc gcgttccgcg acggcgacgt gatccggcga tcgttcgtcg cggctctcga
     5041 cgcactcgtt gccggcggga gctacgtcgg catcgccacg cacgacgaat ggctgatcca
     5101 ggaggcgctg acgcgcgcgc gcgcgctcga cgagagtgcg tctgagttcc agatgctgct
     5161 cggcgtccgc gagaaccgtg cctcggagct cgtcgctgac ggccacccgc tacgggtgta
     5221 cgttccgttc gggcgacgct ggtacgagta ctcgctgcgg cggctgcagg aaaacccggc
     5281 ggtggcgggg cacgtcgcgg ccggcgtgct gcggcggacg ctcccgaagc tctagccgag
     5341 tgccttgggc ttcgccgcgc cggggccgat ccagcactgc agggcggccc cgaggtagta
     5401 ctgggtggcc ttgcggaacg tcgcccgtcg gtaggcgacg accttgcctc cggaacggag
     5461 cttgaggatc acacgcatgc ccttgatctt cgttcccggc ttgagcatca cgcggcatgt
     5521 gagatcgcgc ttcgcgggcg tgcggacggt gcaacgaccg atcgtgctcg agcctcgcag
     5581 caccttgatc tcgatcttgc cggcccgtgc gctccgcgag ctgatcagca acgagcgctt
     5641 ccgcgcagcg aggaagggcc gttgcagcag gttgcgccgc ttggccttct tcgccttcgt
     5701 tccctcggct ggcgtcgcgt caccggctgt ggcctgcccg tcctcgacgg cggcgggcgc
     5761 cgcaggcggc ggtgggctcg cagcgggagg cggcgcgggc gaagcaggtg gcggcggcgg
     5821 cgcagcgggc gggggaggtg aggcaggagg cggaggaggc ggcgggttgg gcgttggtgc
     5881 cggaggctgg gtcgactgcg cctcgattcg gatcgagacc tcgtcgtagg ctccaacact
     5941 cgcagctcgg actgtcaccg aatccggcgt gggtggctgc ggcggagcgg tgtagaggcc
     6001 ggaagacgag atcgtgcccg tcgacgcgtt gccgccttgc acgccgttca cgctccacgt
     6061 gacgttcggc ggatcgttca gcacggtcgc cgcgagctgg acgctcgtcc cggcgagcat
     6121 actcgccggc gcgcccgaga tcgcgacggc cgggtcgatc gagcttacgg agagggacac
     6181 ggttgcgacg gcgggcgacc gagggaagcc actcgacgag tcgtaggcga cgtacgagaa
     6241 cgaatccgac ccgctgaagc cggaggccgg tgtgtacgtg accgtcggaa gcgggaaggg
     6301 gttgccgacg ccttggtcga gcgtcccgtg ctgcggggcg ccgacgatct cgtaccggat
     6361 cggcccgggc gacccgtacg agtccgctgc cagctggatc tgggtggtac cgcttgcctg
     6421 caccgatcgc gaccttgcga cgtagccggg caggaccgac ggcgtgtctg cgaaggcgac
     6481 cgggttcgac tcgtccgcct ccaccacggt gtcgtcaggg tctgcctgag acgcgagcca
     6541 gtaggtgccg ggctgcacgt cgctcacgtc gacccactga aaggcgagcg tccacggata
     6601 tctgtcgcgc caccccgccg agatgcccat catcacttcg ggcgcctgtg tctgcaagcg
     6661 ctggcagaac gacggcgcat cgtagacccg gctgggtggc ccgacggcgt cgaccttctc
     6721 cgagtcgata aggcagaaac cgaccttctg ccccggcgcg acgaccgcgc tcgtggtcga
     6781 gtcgtagagc atgtccgagg ccgcggagcg gaagtgccag tggttgtgtc cgtcggtcgt
     6841 ctcgtagatc agccacggcg acttggaggc gtcgtcacgg ctcgagccgc cggtctggaa
     6901 aatcctctgg aacgtgtcgg tcatctccag gttgaccggg ctccggcccc agatctcgag
     6961 cggtccggga cctcggttgt ggacgaagcc gtcaaagcgc agcaggagcc gcccgcccgt
     7021 cgcgtcccag tacgtctgga ggcgcgggtt ctccggcggg tcggagacga ggtcgggcag
     7081 gttgtcgccg ggctcgacgc ccggcgccgg tccagccgcc aacagagtcc ctgtcagcgc
     7141 ggcggcggcg agtacgagca gagcgaaccg cctccgccag cgcgacctct ccccgctcca
     7201 gcgcacagtc tcagttaagt ggctcttgcg gacggcgtcc agaaccctcc ccacttgggg
     7261 aggtctgaac agcgcacttt gggtgtcttt tcgtccgcgg tcaggccgag atcgagaagc
     7321 cgccgtcgac gacgagcacc tgcccgcgga ccatctcggc gtcgggcgag cagaggaaga
     7381 gcactgcgcc ggcgatgtcg gccggcgtca cgatgcggcc ggctgcgttg tccctcccca
     7441 tgtcgagcat cgcctcccgg ttggggaagt actcgagcgc tcccgtctcg acgacccctg
     7501 ccgagacggc gttcacgcgg atgccgctcg gcccaagctc gatcgccagg tagcgcacga
     7561 gggcttcgag tgccgctttc gacgtgccga cgagcgcgta gttctcgagc acgcgcaccg
     7621 agccgaggct cgacagggcg acgatcgaag acccggcggg catcgaggga gcggcggcgc
     7681 gggtgagtga gaggagcgcc cgcgcgttcg tgccgtgcgt ccagtcccag tgcttgtcct
     7741 cggtctcgag agctgggcga atgacgcccg tggccgcgtt gtggatgaag acgtcgagcc
     7801 ggccgagctc ggcaacctcg gacgcgatcc gctccgacgc gacgttgccg cggacgagca
     7861 ccgggtcggc gcccaggtcg cggatctctg ctgcggtctc ttcggctgcc gagtcgctgc
     7921 gcatgtagcc gatcgcgatt cgcgccgctc cctcgcgggc gaagcgcaag cagatctcct
     7981 tgccgatgcc tcgtgagccg ccggttacga gaaccgactt gcctgcgaag ctcactgctc
     8041 gttgcgaggc agcggctcgt aggccggtgc cgaggagggg
//
LOCUS       DBNGNK010000227        19457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1545987,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000227 DBNGNK010000000
VERSION     DBNGNK010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1545987"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1185)
                     /locus_tag="AC150V_06685"
     CDS             complement(19..1185)
                     /locus_tag="AC150V_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR3762645.1"
                     /translation="MTTRNGSGNGRARAKDKVRVAIIGVGNCANSLVQGVEYYKDASP
                     DTFVPGLMHVDLGGYHISDIEFTAAFDVVKGKVGFDLADAIWAHPNDTIKFSKVPKTG
                     VTVSRGMTHDGIGKYLAEVVEKAPGETDDVVGILKETGTDVVVNYLPVGSEMATKWYT
                     EQILDAGCAMVNCMPVFIAREHYWHKRFEERGVPIIGDDIKSQVGATITHRVLTSLFR
                     ERGVHLDRTMQLNVGGNSDFLNMLERDRLESKKISKTNAVTSMLDYDLGEGNVHVGPS
                     DHVPWLTDRKWAYIRMEGSAFGDVPLNAELKLEVWDSPNSAGIVIDAVRLTKLALNNG
                     IAGALEAPSSYLMKSPPKQIVDDEAHELTERFITKNALTAGGSKRSQKRPSTKA"
     gene            complement(1568..2245)
                     /locus_tag="AC150V_06690"
     CDS             complement(1568..2245)
                     /locus_tag="AC150V_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3762646.1"
                     /translation="MTGADGGRGTPPRVSQFDRVKQEYMEAAREILRRSMAGVAKTKL
                     TPDMLTVAGLVLCIAGAVLVGFEERNEKLFFWLGAIFFIAGSVADILDGALARAASKG
                     TVFGAFLDSTFDRLGEAAMLTAIGLVFMRDGNEVALVAAFAAVIGSLLVSYTRAKAES
                     MGLRGDVGIGSRLERVAIISVGLFVAPWGWLQWPVYFLAAIAWITVVWRMFFVRRQLR
                     EIAEQAG"
     gene            complement(2242..2985)
                     /locus_tag="AC150V_06695"
     CDS             complement(2242..2985)
                     /locus_tag="AC150V_06695"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3762647.1"
                     /translation="MTTHAASLGRRLPRGWSHLGLQFAIWVGFYFAYQVARGAADRSV
                     ATAFEHGRRIIEFQYSLGGLFEPTLQGVVERSWLLLEATAYTYWLSQFAVVGIALLWV
                     YFARHERFYDFRNTLIVANVLGLIGYVLVPTAPPRMFPEWGFTDTLAEHAAVSHESVS
                     ALANPYAAMPSLHAMDALIVTVAMVLIVRSTLAKVAWLLWAPWVWFTVMSTGNHYWLD
                     VLVGIGLAGVGAAIVFGPRALAARRQRAA"
     gene            complement(3003..4031)
                     /locus_tag="AC150V_06700"
     CDS             complement(3003..4031)
                     /locus_tag="AC150V_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR3762648.1"
                     /translation="MSGDLSTTYLGLELDSPVVASASPLTGKLSSLKALEAAGVGAVV
                     LPSLFEEQIEHEEIELDAVLEHGAHSFGEALSYFPELEDYNTGPEQYLEHVADAKSEL
                     GIPVIASLNGASEGGWLRYAHLLQEAGADAIELNVYAVEVDPSAPADVVEERTLALVR
                     AVREAVSVPVAVKVGPFYSAFAHMALRLAEAGADGLVLFNRFLQPDIDLETLSVSPEL
                     HLSTSEELRLPLRWIAILRGRIDVDLAGTGGAHTADDVIKLLLAGADVVMIASALLLH
                     GEGHVKNLRTGIERWLIDNEYDSVDQLKGSMSQAAAPDPAAFERSQYMRALVSYAPDL
                     GGRGTTRR"
     gene            complement(4028..7573)
                     /gene="nifJ"
                     /locus_tag="AC150V_06705"
     CDS             complement(4028..7573)
                     /gene="nifJ"
                     /locus_tag="AC150V_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR3762649.1"
                     /translation="MGARATIDGNEACAWVAYRASEVIAIYPITPSSPMGELADAWSA
                     AGVPNAWDAVPQVVEMQSEGGAAGAVHGALQGGALATTFTASQGLLLMLPNMFKIAGE
                     LSPTVFHVAARTVATHALSIFGDHSDVMAARAIGFGMLCSSSVQEAQDLAAVAHMTTL
                     ASRVPFLHFFDGFRTSHEVNTIERLDDEMLRALLDEAQVHGHRARALTPDAPVLRGSA
                     QNPDVFFQAREAANPYYAAVPEIVDEAMGSFAGQSGRRYRLFEYVGDPKAERVLVVMG
                     SGSAAAQEAVEALAEQGEKVGLVKVRLFKPFAADAFLAALPPTAQRIAVLDRTKEPAA
                     LGEPLFQDVATAILERGGEPPLVTGGRYGLASKEFTPAMAAAVFAELARPKARRRFTV
                     GITDDVTHLSLEVERQFSTEPADVVRAVFYGLGADGTVGANRNSIAIIGEQAGLHAQG
                     YFVYDSKKAGSVTISHLRFGPRPIDSPYLIERAGFVACHQFGLLDKLDVLDVADEGAT
                     VLLNAPFGRDEVWEHLPHEVQEQIIQKGLRLFVVDATRVAREAGLGRRVNTVLQTCFF
                     ALADILPVDDAVAAIKEAIGKSYGKRGRAVLERNYLAVDRALDGLQEVDVPSLVTATH
                     RRQPPVPSVAPDFVQRVTAAMIAGRGDQLPVSALPVDGTFPTGTARFEKRSIAEEIPI
                     WDPSICIDCAKCALVCPHAAIRMKVYEPDALDGAPDGFLAKDWRDRHLPGMKMTIQVA
                     PEDCTGCGICVEVCPAFSKEEVKHKSINLEPMRDHLETERERYEFFLDLPEIDRTTVE
                     RDTIKGSQMLEPLFEYSGACEGCGETPYLKLLTQLLGDRILVANATGCSSIFGGNLPT
                     TPWSTNGEGRGPAWSNSLFEDNAEFGLGMRLALDSQEAQARALVAELAPDLADAILEA
                     EQLSEDGIAAQRERVRELRERLNGMNGPRPRRLEALVGALVRKSVWIVGGDGWAYDIG
                     FGGLDHVLASGRDVNVLVLDTEVYSNTGGQASKATPRGAVAKFAAGGKQTRKKDLGQI
                     ASAYGDVYVAQIAMGANNAQTVKALAEANSWPGPSLVIAYSHCIAHGIDMGKGMEQQK
                     LAVDSGYWPLWRYDPRQSGPGEHPFHLDSRKPKVPVSKFTEREARFAMLARARPEEAA
                     RLGALAQHDVDERWHVYEQLANVEHEPAEDEEEAE"
     gene            complement(7651..8097)
                     /locus_tag="AC150V_06710"
     CDS             complement(7651..8097)
                     /locus_tag="AC150V_06710"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3762650.1"
                     /translation="MGLRPRTVRHVSRAVRSGRIVLYLSGTTVLLALVAAVVMRLSND
                     AAFPTMGISLWWAIVTLTTVGYGDVVPSDALGRTIGAVLMTLGVTYIAFMTAVITSVL
                     ISLNTARLSREAEDDGPETPAADVDLQSTMRRIEQRLAAIEQKLDG"
     gene            complement(8180..9229)
                     /gene="moaA"
                     /locus_tag="AC150V_06715"
     CDS             complement(8180..9229)
                     /gene="moaA"
                     /locus_tag="AC150V_06715"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR3762651.1"
                     /translation="MASIEATAQRYALGVSAVKDTLGRHVRDLRISVTDRCNFRCTYC
                     MPKSVFGRDYEFMPRRELLTFEEIERVARMFVGHGVQKIRITGGEPLLRRDLEVLVSQ
                     LAAIEEELDLTLTTNGALLPRKAEALRDAGLTRVTVSLDSLDDETFRDMNDVDFPVAA
                     VLEGIDAAAGVGLPVKVNAVVKRGVNDDGLVELARHFRGTGHPVRFIEFMDVGATNGW
                     RLDDVVPAGEIVRRIDEVFPLEPVEPAYRGEVARRWRYRDGAGEIGVIASVTQPFCGD
                     CTRARISADGKLYTCLFAVRGTDLRELLRSGATDEDLAGTIGGVWAARSDRYSELRTA
                     ETASTPKVEMSYIGG"
     gene            9210..9710
                     /locus_tag="AC150V_06720"
     CDS             9210..9710
                     /locus_tag="AC150V_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762652.1"
                     /translation="MASMDAILPVWDVRSRHGVRLACEPERALAAALGVPVASDPLVR
                     ALLQARGLGARGTVGEAFSWMGFDTLVESPTEFVAGASGRPWRPRGHIRPFEEAAPGT
                     VRIAASIRASAAADAGALLETETRVLAVDDAARIAFARYWRVVGPFSGLIRRRWLGAA
                     ARAIGV"
     gene            9707..10612
                     /locus_tag="AC150V_06725"
     CDS             9707..10612
                     /locus_tag="AC150V_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR3762653.1"
                     /translation="MTTGRLAPLLALVGVTAVWGYTFVQVKDAIALYPLFAFLAVRFL
                     IGTVVLVPPAWSRVRPLGRRGVLVGLGIGIVLAAGYAFQTAGLERTTASSAGFITGLF
                     VVITPLLATAFFGVRIGVVSWLGVAVATVGLGLLSGGGAGEIVGDALVLVAALALSVH
                     IIVLERYAPGYDVVALALLQMLAAFLAFSVIAVATGQLEVPRGSTVWAALIVTGVFAS
                     ALGFLVQTWAQQRTTATRTALALTMEPVWAGFFGYVLAGDRLGVAGWVGAALIMAGIV
                     LSEPAAAESLRRLVRGIPASRTTRK"
     gene            complement(10727..11800)
                     /locus_tag="AC150V_06730"
     CDS             complement(10727..11800)
                     /locus_tag="AC150V_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3762654.1"
                     /translation="MNDGRVEAYACLLVDRCVHAQPGWQVLVATTVEALPLAGVLSRL
                     LAERGAWCLQRITTGAPFPADLDWIEAAPDELAGSPAPLEQTVIDSVDASIFVLAPSL
                     GRQPATPTAAAALRAQASAYRERQRRGEIPSVRCDFPCPAYAERAGMTLHEFEALFYD
                     ACIRDWESESRAMRPVLERFDASSEVRIRGDRTNLRLSLAGREGAIDDGILNLPGGEV
                     YYSPVEDSLEGEIAFDVPTVSVGGPVEGVRLRFESGTVVDAAAAVGEDLLLAALDTDA
                     GARRAGEFGIGCNAGLDRPLRNVLFDEKIAGTVHLALGYGFPPLGGRNVSAIHWDLVK
                     DLREGGELLADGAVVQRDGRWLL"
     gene            11903..14302
                     /locus_tag="AC150V_06735"
     CDS             11903..14302
                     /locus_tag="AC150V_06735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR3762655.1"
                     /translation="MVAARTLILGCLVAVASGLLLGGASGSSRVSQATAELGWRVEEK
                     PFRVVVRHAGKDLLAQAVTAERGPGRRMSYKLESGSVHTLTRLLSQRSSRGKTVYRVA
                     TDEPGRAAVVTVSRTARGIRVSLRLFPEKDVIEVYEAFEGTRGEHFLGTGQRTDFVDL
                     RGTVVPLKVWNICASTLVVPFFLSSRGYGVRLRTTAVGMLSFPGATWDARFRCDLGTD
                     PCEQAGPLVSTRTCLKANRLDYDVYAGTPAQIVRAHSAAIGLPSLPPPNQFALIKWRD
                     EIAGPEELFDDIEQLRKRQIPIGWVILDNPWEKGAFASGCYGALEFDPQRYPNPKQMI
                     SRIHDLGVRFMLWVSPQAPNKDGCPPPPYQAGHFVGDDEIFVVDLTLPDARSEYRRRL
                     DALVALGVDGFKGDRGDELDLEDQTLHAGSGLRQHNRIPVLFSDAIAGALRKVHGRNY
                     GTLNRAGYTGAQTRLHGLFGLDLPHDFRGLQEAVRMSQNAGVSGFPFWGSDIGGYDGD
                     SEITSEVLVRWAQFGALTPVFEIGGQGLTSRFWELGDPTVALFRRAAIMHYELFPYLY
                     ELARNASRTGLPIVRPLGFRYPADEQAWRADQQLLVGNDLLAAPVTSGTRSGNDLAGP
                     ATRWKVYVPAGSWVDVHRGTSARGPLTLTRSTPISELPLYLRAGAAIPFNSRSPAVWA
                     KPWRVNDLNRAGRAGWLYAPGTSPTRAAGGRAGTFVAAREGRTVALRVEAGQRETQVI
                     VLARARPCRVAIGGRPVAEANAATLRSRSSGWTFRRGSFGGITLKLTAPASAATVTFC
                     D"
     gene            14458..16368
                     /gene="dnaK"
                     /locus_tag="AC150V_06740"
     CDS             14458..16368
                     /gene="dnaK"
                     /locus_tag="AC150V_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR3762656.1"
                     /translation="MAKTIGIDLGTTNSAMAVLEGGEPTVIPNAEGGRTTPSVVAFTN
                     DGQRLVGAPARRQQVTNPENTIFSIKRFVGRKWDEVSEEMTIVPYKVVKGENGDARVE
                     ANEREYAPPEISAMILQKLKADAEAYLGEPVTDAVVTVPAYFNNAQREATKDAGKIAG
                     LNVQRIINEPTAAALAYGLDKEGADQTILVFDLGGGTFDVSVLELGDGVFEVKSTNGD
                     NHLGGDNFDKALVDWMTAEFKKHQGIDLSQDKMALQRLYEAAEKAKIELSSTMTTQIN
                     LPFITATQEGPKHLDLPLTRAKLNELTQELLQRTVEPTKQALADAELSAGDIEHVVLV
                     GGMTRMPAVQEKVKELIGKEPHKGVNPDEVVAVGAAIQGGVLKGEVKDVLLLDVTPLS
                     LGIETKGGVFTQLIERNTTIPTRKAETFSTAEDNQPSVEIHVLQGESEMAAFNKTLGK
                     FQLVGIPPAPRGMPQVEVSFDIDANGIIHVTAKDLGTGNEQQIRIEGGSGLSEEEVNR
                     MVTEAEAHAGEAHKLRELADARNLAETLAYQTEKSLNEHRDKIEEAEAGTIEGRIMEL
                     RQALEGSDLADIQSKTQALQEAAQPLATAVYAEASQQAASGAGNGGTTEDEVVEDADY
                     EVIDEDEAATKS"
     gene            16365..16895
                     /gene="grpE"
                     /locus_tag="AC150V_06745"
     CDS             16365..16895
                     /gene="grpE"
                     /locus_tag="AC150V_06745"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR3762657.1"
                     /translation="MTEDRLASEETVEEDSVDPAGASSGEAALDERMAEVEGQRDEYL
                     ADLQRLAADFDNYRKRVAREQAAVVARAHERLVGHLLPVLDDLERALEAAEAHDEAQV
                     IEGVRLVRESLIAALEREGLEEIGADGKFDPHVHEAMLTQPAPDVEQGDVVQVLQKGY
                     RLGDHVLRPARVVVAE"
     gene            16900..18000
                     /gene="dnaJ"
                     /locus_tag="AC150V_06750"
     CDS             16900..18000
                     /gene="dnaJ"
                     /locus_tag="AC150V_06750"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR3762658.1"
                     /translation="MTAVKDPYDVLGVSKAASDEEIKKAYRKLARESHPDRNPGDEAA
                     ESRFKDIQTAYDLLSDAERRKEYDAYGAAGPGAGPFGPGGYRNVRVEGTDFDLGDLFG
                     GIFGRGARAQARPQPERGVDLESHVRLSFEDALKGVQVRVPVEVDTACSVCGGTGAEP
                     GTAPRTCPDCDGSGVQADSHGLFALSQPCRRCRGNGTIVDKPCRSCRGSGSERSTKRY
                     SVKIPPGAKDGTRVRLKGKGEPGRNGGPPGDVFVVVEVEASPLYERRDADLILDVPVT
                     YPEAALGASVEIPTPYGSVALKVPAGSENGKLLRVKNRGAPKLKGNGRGDLLARLRIT
                     VPRKLSKAEKEALEGYQKSSRERPREHFTEVR"
     gene            18004..18366
                     /locus_tag="AC150V_06755"
     CDS             18004..18366
                     /locus_tag="AC150V_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014516112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein transcriptional repressor
                     HspR"
                     /protein_id="HIR3762659.1"
                     /translation="MPDRPRYMISVAAELVDMHPQTLRMYESKGLIAPARTPGGTRLY
                     SEKDLERLRLIQRLTTELGLNLAGVEHVLRLEDELRRLRLRMQRLERELRQEITEVHR
                     SYRREVVLWEAPKPATRR"
     gene            18425..>19457
                     /locus_tag="AC150V_06760"
     CDS             18425..>19457
                     /locus_tag="AC150V_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR3762660.1"
                     /translation="MDFNKLTIKSGEAVAGAQELARRLGNPELHPEHLTIALLDQELP
                     RTLVERTGASPDSLRAEAEAALRGKPSVSGGNVQPQVSVPFRRVLDGAFEWMKKLEDD
                     FVSVEHLLLALGLVADDAVLNVLKEVRGGQRVTSQDPEGGYQALEKYGRDLTTLAEAG
                     KLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLAQRIVAGDVPEGLK
                     GKRVWALDVGALLAGAKYRGEFEERLKAVLQEITAAEGEIVLFIDELHTIVGAGAAEG
                     AVDAANMLKPLLARGELRAIGAPTLDEYRKHLEKDAALERRFQPVFVGEPSVQDTISI
                     LRGLKERYEV"
BASE COUNT         3101 a         6581 c         6786 g         2989 t
ORIGIN      
        1 aatagagagt cgaagaggct atgccttcgt cgacggcctt ttctgcgacc gcttcgagcc
       61 gccggccgta agcgcgttct tggtgatgaa gcgctcggtc agctcgtgcg cctcgtcgtc
      121 gacgatctgc ttcggcggcg atttcatcag gtaggagctc ggggcctcga gcgcgccggc
      181 gatgccgttg ttcagcgcga gcttcgtcag gcgcacggcg tcgatcacga tgccggccga
      241 gttgggcgag tcccacacct cgagcttcag ctcggcgttg agcggtacgt cgccgaaggc
      301 ggagccttcc atccggatgt acgcccactt ccgatcggtg agccacggga cgtggtcgga
      361 ggggccgacg tgcacgttcc cctcgccgag gtcgtagtcg agcatcgacg tgacggcgtt
      421 cgtcttcgag atcttcttcg attccagccg gtcgcgctcg agcatgttga ggaagtcgga
      481 gttgccgccg acgttgagct gcatcgtccg gtcgaggtgt acgccgcgct cgcggaacag
      541 gctcgtgagc acgcggtgcg tgatcgtggc gccgacctgc gacttgatgt cgtcgccgat
      601 gatcggcacg ccccgttcct cgaatcgctt gtgccagtag tgctcgcggg cgatgaagac
      661 gggcatgcag ttcaccatcg cgcagccggc gtcgaggatt tgctccgtgt accacttggt
      721 ggccatctcc gagccgacgg ggaggtagtt gacgacgaca tcggtgccgg tctccttcag
      781 gattccgacc acgtcgtcgg tttctccagg agctttctcg acgacttcgg ccaggtactt
      841 cccgattccg tcgtgcgtca tcccccgtga gacggtcact cccgtcttgg ggaccttgga
      901 gaacttgatc gtgtcgttgg gatgcgccca gatcgcatcg gcgaggtcga agccgacctt
      961 gcccttcacg acgtcgaaag cggctgtgaa ctcgatgtca gagatgtgat agccgccgag
     1021 gtcgacgtgc atgagtcccg gcacgaacgt gtcgggcgag gcgtccttgt agtactcgac
     1081 gccctgcacg agcgagttgg cgcagttgcc tacaccgatg atggcaacgc gcaccttgtc
     1141 cttggcacgc gcacggccgt tcccggagcc attcctggtg gtcatcgtct ttcccttctt
     1201 cctgtctcgt ggggcgggaa tgccgagtcc gttcgcgaga gcggcgacca tgacgtcgtt
     1261 ggcatgggcg ccgctgctcg ccgcctcctc gtcgatcgcg ttcttgagct cgttcggcat
     1321 gcgaagcacc atcacgggac tggagcccgg aagcgggctg cggcgtcccg tcggctgata
     1381 gctgatgccg agctcgtcgg caaggagacc gaccgcgacg tcgttcaggc tcacgcctct
     1441 gcgcgccgtc tcgcggacga gcgcgctctt cagcgaggaa ggggcgcgaa cgagtacgct
     1501 tcgtctcttc gaagtgctag cagccaaact gctagcagta tagcccggcg gtgagaaccg
     1561 cgctctgcta gccggcctgc tctgcgatct cgcgcagctg ccggcggacg aagaacatcc
     1621 gccagaccac cgtgatccag gcgatcgccg ccaggaagta gacaggccac tgcagccagc
     1681 cccacggcgc gacgaagagc ccgaccgaga tgatcgcgac ccgctcgagc cgcgagccga
     1741 tgccgacgtc gccgcgaagc cccatcgact cggccttcgc gcgcgtgtag ctgacgagca
     1801 gcgacccgat cacggctgcg aacgcggcga cgagcgccac ctcgttgccg tcgcgcatga
     1861 agacgagccc gatcgccgtg agcatggctg cttcgccgag ccggtcgaag gtggagtcga
     1921 ggaaggcgcc gaagaccgtg cccttgctgg cggcgcgcgc gagcgccccg tcgaggatgt
     1981 ccgccaccga gcctgcgatg aagaagatcg cgccgagcca gaagaacagc ttctcgtttc
     2041 tctcctcgaa gccgaccagg accgcgccgg cgatgcagag gacgagcccc gcgacggtca
     2101 gcatgtcggg cgtcagcttc gtcttggcga cacccgccat cgaacggcgc aggatctccc
     2161 gcgccgcctc catgtactcc tgcttgacgc ggtcgaactg gctgacccgc ggcggcgtac
     2221 cgcgaccgcc gtcggcgccc gtcatgcagc tcgctgtcgg cgcgcggcga gggctcgggg
     2281 gccgaagacg atcgcggccc cgacgccggc gagcccgatt ccgacgagaa cgtcgagcca
     2341 gtagtggttg ccggtcgaca tgaccgtgaa ccagacccag ggtgcccaga gcagccacgc
     2401 gaccttcgcg agcgtgctcc ggacgatgag aaccatcgcc acggtcacga tgagcgcgtc
     2461 catggcgtgc aggctcggca tcgccgcgta cgggttggcc agcgcggaga cgctctcgtg
     2521 cgagaccgcc gcatgctcgg cgagagtgtc ggtgaagccc cattccggga acatccgcgg
     2581 cggagccgtc ggcacgagca cgtagccgat caggccgagc acgttcgcga cgatgagggt
     2641 gttcctgaag tcgtagaagc gctcgtgccg ggcgaagtag acccagagca acgcgatccc
     2701 gacgacggcg aactgcgaca gccagtacgt gtaggcggtc gcctcgagta ggagccacga
     2761 acgctcgacg acaccttgca gggtcggctc gaacagcccg ccgaggctgt actggaactc
     2821 gatgatccgc cgcccgtgct cgaaggccgt cgcgacgctg cggtctgctg cgccacgcgc
     2881 aacctggtag gcgaaataga agccgaccca gatggcgaac tggaggccga gatgcgacca
     2941 gccacgtggt aggcgccggc cgagggaggc ggcatgcgtc gtcacgagaa ctgaaccgta
     3001 tcttagcggc gggtcgtgcc gcggccaccc aaatcggggg cgtacgacac gagggcccgc
     3061 atgtactgcg atcgctcgaa cgccgccgga tcgggcgctg ctgcctggct catgctcccc
     3121 ttcagctgat ccaccgagtc gtactcgttg tcgatcagcc atctctcgat gcctgttcgc
     3181 aagttcttca cgtgtccctc tccgtgcagg agcagagccg acgcgatcat caccacgtcc
     3241 gcgccggcca gcaggagctt gatcacgtcg tctgccgtgt gcgctccgcc ggttccggcg
     3301 aggtcgacgt cgatgcgccc gcgcaggatg gcgatccagc ggagcggcag ccggagctcc
     3361 tccgacgtcg agagatggag ctcgggcgag acggacagcg tctcgaggtc gatgtcgggc
     3421 tgaaggaacc ggttgaacag gacgagcccg tccgcacccg cctctgccag acgcagcgcc
     3481 atgtgggcga aggcgctgta gaaggggccg accttgacgg cgaccggcac cgagacggcc
     3541 tcccgcacgg cgcggaccag cgccagggtt cgctcctcga ccacgtcggc gggggcgctc
     3601 ggatccacct cgaccgcgta gacgttgagc tcgatcgcgt ccgcaccggc ctcctggagc
     3661 agatgcgcgt agcgcagcca gccgccctcc gaggcgccgt tgaggctggc gatcacggga
     3721 atgccgagct cggacttcgc atccgcgacg tgctcgaggt actgctcggg ccccgtgttg
     3781 tagtcctcca gctcggggaa gtacgagagc gcctcgccga agctgtgcgc cccgtgctcg
     3841 agcaccgcgt cgagctcgat ctcctcgtgc tcgatctgct cctcgaacag cgacgggagc
     3901 acgacggcgc cgacgccggc cgcttcgagc gccttgaggc tcgagagctt gccggtcaag
     3961 ggcgacgcgg aggcgaccac gggcgagtcg agctcgagcc cgagataggt ggtgctgaga
     4021 tcgccgctca ttccgcttcc tcctcgtcct ccgccggctc gtgctcgacg ttcgcgagct
     4081 gctcgtagac gtgccagcgc tcgtccacgt cgtgctgcgc gagcgcaccc aggcgggctg
     4141 cctcctctgg gcgggcacgg gccagcatcg cgaagcgcgc ctcgcgttcg gtgaacttcg
     4201 acacgggaac cttgggcttg cgcgagtcga ggtggaaggg atgctccccg ggccccgact
     4261 ggcgcgggtc gtagcgccac agcggccagt agcccgagtc gaccgcgagc ttctgctgtt
     4321 ccatgccctt gcccatatcg atcccgtgcg cgatgcagtg gctgtacgcg atcaccagcg
     4381 agggcccggg ccacgagttc gcttcagcga gagccttcac ggtttgggcg ttgttcgcac
     4441 ccatcgcgat ctgcgccaca tagacgtcgc cgtaggccga cgcgatctgg ccgagatcct
     4501 tcttccgcgt ctgcttgccg ccggcggcga atttggccac tgcgccgcgc ggcgtcgcct
     4561 tcgatgcctg gccgccggtg ttcgagtaga cctccgtgtc caggacgagc acgttcacgt
     4621 cgcgtccgga cgcgaggacg tgatcgagcc cgccgaagcc gatgtcgtag gcccagccgt
     4681 cgccgcccac gatccagacc gacttccgga cgagcgctcc gaccagcgct tcgagtcgcc
     4741 tcggccgagg gccgttcatc ccgttcaggc gctcgcgcag ctcgcgcacc cgctcccgct
     4801 gcgcggcgat cccgtcctcg gacagctgct ccgcctcgag aatggcgtct gccaggtcgg
     4861 gtgcgagctc cgcgacgagc gcgcgcgcct gcgcctcctg tgagtcgagc gcgagtcgca
     4921 tgccgagacc gaactccgcg ttgtcttcga acagggagtt cgaccaggcc ggcccacgcc
     4981 cctcgccgtt cgtgctccag ggagtggtcg gcaggttgcc gccgaagatc gacgagcagc
     5041 cggtcgcgtt ggcgacgagg atccggtcac cgaggagctg ggtgagcagc ttcaggtacg
     5101 gcgtctcgcc acagccctcg caggcgcccg agtactcgaa cagcggctcg agcatctgtg
     5161 accccttgat cgtgtcccgt tcgacggtcg tgcggtcgat ctcgggcagg tcgaggaaga
     5221 attcgtaccg ctcgcgctcc gtctcgagat ggtcgcgcat cggctccagg ttgatcgact
     5281 tgtgcttcac ctcttccttc gagaaggccg ggcaaacctc gacgcagatc ccacagccgg
     5341 tgcagtcctc gggcgcgacc tggatcgtca tcttcatccc cggcagatgg cggtcgcgcc
     5401 agtccttggc gaggaacccg tcgggcgctc cgtcgagcgc gtccggctcg tacaccttca
     5461 tgcggatcgc agcgtgcggg cagacgaggg cgcacttcgc gcagtcgatg cagatcgagg
     5521 ggtcccagat cgggatctcc tctgcgatcg agcgcttctc gaagcgggcc gtcccggtcg
     5581 ggaaggttcc gtccacgggc agcgcgctca cgggaagctg gtcgcctcgc ccggcgatca
     5641 tcgcggcggt gacgcgctga acgaagtccg gcgcgacgga cgggacagga ggctggcgcc
     5701 gatgggtcgc ggtcacgagg gacgggacgt ccacctcctg caggccgtcg agggcgcggt
     5761 cgacggccag gtagtttcgc tcgaggacgg cacggccccg ctttccgtag ctcttcccga
     5821 tcgcctcctt gatggcggcg accgcgtcgt cgacgggcag gatgtccgcc agggcgaaga
     5881 agcaggtctg caacaccgtg ttgacgcggc gcccgagccc cgcctcgcgc gcgacgcggg
     5941 tggcgtcgac gacgaagagg cgcagtccct tctggatgat ctgctcctgc acctcgtgcg
     6001 gaaggtgctc ccagacctcg tcgcgcccga acggggcgtt gagcaagacc gtcgctccct
     6061 cgtcggcgac gtcgagaacg tcgagcttgt cgagcaggcc gaactggtgg caggcgacga
     6121 agccggcgcg ctcgatcagg tacggggagt cgatcggccg cggtccgaag cggaggtgcg
     6181 agatcgtgac cgagccggct ttcttcgagt cgtagacgaa gtagccctgc gcgtgcagcc
     6241 cggcttgctc gccgatgatc gcgatcgagt ttcggttggc gcccacggtg ccgtccgcgc
     6301 ccagcccgta gaagacggcg cggacgacgt ccgccggctc tgttgagaac tggcgctcga
     6361 cctcgaggga gaggtgcgtg acgtcgtccg tgatcccgac ggtgaaacgg cggcgtgcct
     6421 tcggccgggc cagctcggcg aagacggccg ccgccatcgc cggggtgaat tccttcgacg
     6481 caagcccgta ccgtcctccg gttacgagcg gcggctcacc gccgcgctcg aggattgccg
     6541 tcgcgacgtc ctggaacagc ggctcgccga gcgccgccgg ctccttggtc cggtcgagga
     6601 cggcgatgcg ctgggccgtc ggcggcaggg ctgcgaggaa cgcgtcggcg gcgaagggct
     6661 tgaagaggcg gactttcacc agaccgacct tctctccctg ctcggcgagc gcctcgactg
     6721 cctcctgcgc ggcggcgctg cccgagccca tcacgacgag cacgcgctcc gccttcgggt
     6781 cgccgacgta ctcgaagagc cgatagcggc ggccagactg ccctgcaaac gaccccatcg
     6841 cctcgtccac gatctccggc accgcggcgt agtacgggtt cgccgcctct cgcgcctgga
     6901 agaagacgtc cgggttctgc gctgagccgc gcagcacggg cgcgtccggc gtcagcgctc
     6961 gcgcgcgatg gccgtgcacc tgggcctcgt cgagcagcgc acgaagcatc tcgtcgtcga
     7021 gacgctcgat cgtgttgacc tcgtgcgacg ttcgaaatcc gtcgaagaag tgcaggaacg
     7081 ggacgcgtga ggcaagggtc gtcatgtggg cgaccgcggc caggtcctgc gcctcctgca
     7141 cggacgagga gcagagcatg ccgaatccga ttgcccgcgc cgccatcacg tcggagtggt
     7201 cgccgaaaat cgagagcgcg tgcgttgcga ccgtgcgtgc cgcgacgtgg aagacggtcg
     7261 ggctcagctc gcctgcgatc ttgaacatgt tcggcagcat cagcagcagg ccctgggagg
     7321 ccgtgaaggt ggtcgccagc gcgccgcctt gcagggcgcc gtgaaccgcg cccgccgcac
     7381 cgccctcgct ctgcatctcc acgacctggg gcaccgcatc ccaggcgttc gggacgcccg
     7441 ccgcggacca ggcgtcggcc agctcgccca tcggtgagga aggagtgatc ggatagatgg
     7501 cgatcacctc gctggcgcga taggcgaccc aggcgcacgc ctcgttgccg tcgatcgttg
     7561 cacgggctcc cacgcctccg atgctcgttc accgggcagc ggtcggcatc ggtgccgccg
     7621 cggatcggct ttcggtgtct ccgcaggcgg tcagccgtcg agcttctgct cgatcgcggc
     7681 gagccgctgc tcgattcggc gcatggtgga ctgcagatct acgtcggcag cgggagtttc
     7741 gggtccgtcg tcttcggctt cccgactcag cctggccgtg ttgagcgaga tcaggaccga
     7801 cgtgatgaca gcggtcatga aggcgatgta ggtgacgccg agcgtcatga ggacagcgcc
     7861 gatcgtccga ccgagggcgt cgctggggac gacatcgccg taccccaccg ttgtcagggt
     7921 cacgatcgcc caccagaggg aaatccccat cgtcgggaag gcggcgtcgt tggacaggcg
     7981 catcacgacc gcggcgacca gagcgagcaa cacggtcgtc ccagacaggt agaggacgat
     8041 ccggccggac cgcacggccc gcgacacgtg ccgcacggtg cggggcctga gccccatgat
     8101 cctctccgta tcgaggttct ccgccatggc gcccggttcg gcgcctcggc ccgaagccct
     8161 actgcgggta cacccgccgc tagccgccta tgtaggacat ctcgaccttc ggcgtcgacg
     8221 ccgtctcggc cgtacgaagc tcggagtagc ggtcactccg cgccgcccac accccgccga
     8281 tcgtgccggc gagatcctcg tcggtcgcac cggagcgcag cagctcgcgg agatcggtgc
     8341 cgcggacggc gaagaggcag gtgtagagct tcccgtcggc cgagatgcgc gcgcgcgtgc
     8401 agtcgccgca gaacggctgc gtgaccgacg cgatcacgcc gatctcgccc gcgccgtcgc
     8461 ggtagcgcca gcggcgcgca acctcgcccc ggtaggcagg ctcgaccggc tcgagcggga
     8521 acacctcgtc gatccggcgc acgatctcgc cggcgggcac gacgtcgtcg agccgccagc
     8581 cgtttgtcgc gccgacgtcc atgaactcga tgaagcggac gggatggccg gtgccgcgga
     8641 agtggcgggc cagctccacc aggccgtcgt cgttgacgcc ccgcttgaca acggcgttga
     8701 ccttgacggg caggcccacg ccggccgcag cgtcgattcc ctcgagcacg gctgccacag
     8761 ggaagtcgac gtcgttcatg tcgcggaagg tctcgtcgtc gagtgagtcg aggctcaccg
     8821 tgacacgcgt gaggcccgcg tcgcggagcg cttcggcctt gcgcggcagg agtgctccgt
     8881 tcgtcgtcag cgtgaggtcg agctcctcct cgatcgccgc cagctgggag acgagaacct
     8941 cgaggtcgcg gcggagcagc ggctcgcctc cggtgatccg gatcttctgc acgccgtggc
     9001 cgacgaacat gcgggcgacg cgctcgatct cctcgaaggt gagcagctcg cggcgcggca
     9061 tgaactcgta gtcgcggccg aagacgctct tcggcatgca gtacgtgcag cggaagttgc
     9121 agcggtcggt caccgagatt cggaggtcgc gaacgtgccg gccgagggtg tccttcaccg
     9181 cgctcacgcc gagagcgtag cgttgcgccg tggcttcgat ggacgcgatc cttccagtct
     9241 gggatgttcg cagccggcac ggggtgcgac tcgcttgtga gcctgagcga gccctggccg
     9301 ctgccctcgg tgttccggtc gcatccgatc cgctcgtccg agccttgctc caggcgcgcg
     9361 gtcttggggc ccgcggcacg gtcggtgaag ccttctcgtg gatggggttc gacacgctcg
     9421 tggagtcgcc gaccgagttc gtcgcgggag cgtccggccg gccctggcgg ccacgtggac
     9481 atatccggcc gttcgaagag gcggctcccg ggacggttcg catcgccgcg tcgattcgag
     9541 cctcggcggc cgccgacgcg ggtgcgctgc tcgagaccga gactcgggtg ctggccgtcg
     9601 acgacgcggc gcggatcgcg ttcgcgcgct attggcgggt cgtcggtccc ttctccggcc
     9661 tgatccggcg gcgctggctc ggcgctgccg cgcgggcgat cggcgtgtga ccaccggccg
     9721 actcgctccg ctgctcgcgc tggtcggcgt gaccgcagtc tggggataca cgttcgtcca
     9781 ggtgaaggac gcgatcgccc tctatccgct gttcgccttt ctcgccgtgc gctttctgat
     9841 cggcaccgtg gtgctcgtgc cgcccgcttg gtcgcgcgtg cgcccactcg gtcgtcgcgg
     9901 cgtgctcgtg ggcctcggga tcggcatcgt cctcgcggcc ggctatgcct tccagacggc
     9961 tgggctcgag cggacgacgg cctccagcgc ggggttcatc actggcctct tcgtcgtgat
    10021 caccccgctc ctcgcgacgg ccttcttcgg tgttcgcatc ggcgtcgtct cgtggctcgg
    10081 cgtggcagtc gccaccgttg gtttggggct gctctccggc ggaggggctg gggagatcgt
    10141 aggtgatgcc ctggtgctcg tcgcggccct cgcgctgtcg gttcacatca tcgtgctcga
    10201 gcgctacgcg ccgggctatg acgtcgtcgc ccttgcgctg ctccagatgc tggcagcgtt
    10261 tctcgcgttc tcggtgatcg cggtcgccac gggtcagctg gaggttccgc gaggatcgac
    10321 cgtttgggcg gcgctgatcg tcaccggcgt cttcgcgagc gcgttgggct tcctcgtgca
    10381 gacgtgggcg cagcagcgga cgacggcgac ccgcaccgcc ttggctctga cgatggagcc
    10441 cgtctgggcg ggcttcttcg gttacgtgct ggccggcgat cggctcggtg tcgccgggtg
    10501 ggtgggcgcg gcgctgatca tggccgggat cgtcctttcg gagcccgctg ccgcggagtc
    10561 gctccgccgc ctcgtgcgag ggatccccgc gagccgcacg acccgcaagt aacagactgt
    10621 tacttggcag aaaggggctt gcggaagcgg ctgcacggaa tcaagtaaca gactgttaca
    10681 aacaacaagc ccagtttctt ccctatggcc tccgctggct gtgttcctag agcagccagc
    10741 ggccgtcgcg ctgcacgacc gcgccgtcgg cgagcagctc gccaccttcc cgcaggtcct
    10801 tgacgagatc ccagtggatg gcgctgacgt tgcgtccgcc gagcggcggg aagccgtagc
    10861 ccagcgcgag gtgcacggtg ccggcgatct tctcgtcgaa gaggacgttg cgcagcggac
    10921 ggtcgaggcc ggcgttgcag ccgatgccga actcgccggc ccgtcgtgcc ccggcatcgg
    10981 tgtcgagcgc cgcgagcagc aggtcctcgc cgaccgcggc ggccgcatcg acgaccgtcc
    11041 cggactcgaa gcgaagccgc acgccctcga ccggcccgcc gacgctgacc gtgggcacgt
    11101 cgaaggcgat ctcgccctcc aacgagtcct cgacgggcga gtagtagacc tcaccgccgg
    11161 gcaggttgag gatgccgtcg tcgatcgcgc cctctcggcc ggcgaggctc aggcgaagat
    11221 tcgtccggtc gccgcggatc cgtacctcgg aggacgcgtc gaaccgctcg agcacaggac
    11281 gcatcgcgcg gctctcgctc tcccagtcgc gaatgcaggc gtcgtagaag agagcctcga
    11341 actcgtgcag cgtcatgccc gcgcgctccg cgtacgccgg acagggaaag tcgcaccgaa
    11401 cggacgggat ctcaccacgc cgctgccgct cgcgataggc gctcgcctgc gcgcggaggg
    11461 cggcggcggc cgtgggagtc gcaggttgac ggcccagcga cggagcgagc acgaagatcg
    11521 acgcgtcgac ggagtcgatc accgtctgct cgagcggcgc gggtgagccg gccagctcgt
    11581 cgggcgcagc ctcgatccag tcgaggtcgg ccgggaaggg ggcgccggtc gtaatgcgct
    11641 gcaggcacca ggcgccgcgc tcggcgagca ggcgtgagag cacgccggcc aagggcagtg
    11701 cctccaccgt cgtggcgacg agcacctgcc agccgggttg cgcgtgcacg cagcggtcga
    11761 ccagcaggca cgcgtacgcc tcgacgcgcc cgtcgttcac gaagtcgatc gtacttctcg
    11821 cggaggcgac tgccggtgag ctgccacagg cgcagaggac acccggcgcg ggacggtagc
    11881 gttcagacag gttcgggtcg caatggtcgc tgcgcgcacg ctcatactcg gctgcctcgt
    11941 cgccgtcgcc tccggtctcc tcctcggcgg agcgagtgga tcgagccggg tctcccaggc
    12001 gaccgcagag ctcggctggc gcgtcgaaga gaagcccttt cgcgttgtcg tgagacacgc
    12061 cgggaaagac ctcctggcac aggccgtcac ggccgagcgc ggaccggggc ggcgtatgag
    12121 ctacaagctc gagagcggca gcgtccacac gctcacccgg ctgctctcgc aaagatcctc
    12181 gcgcggcaag accgtctacc gcgttgcgac cgacgagccc ggccgtgccg cggtggtaac
    12241 ggtgagccgg accgcccgcg gcatccgagt ctccctccgc ctctttccgg agaaggacgt
    12301 catcgaggtg tacgaggcct tcgagggaac gcgcggcgag catttcctcg gcaccggtca
    12361 gcgcacggac ttcgtcgacc tcaggggtac ggtcgtgccc ctgaaggtct ggaatatctg
    12421 cgccagcact ctcgtggttc cgttcttcct cagctcccgc ggctacggcg tccggctgcg
    12481 gacgacggcg gtcggcatgc tctcgttccc gggtgctacg tgggacgccc gcttccggtg
    12541 cgacctggga acggacccgt gcgagcaagc cggcccgctg gtctcgacgc gcacgtgcct
    12601 gaaggcgaac cggctggact acgacgtcta cgccggaacg ccggcgcaga tcgtgcgcgc
    12661 ccactctgcc gcgatcgggc taccctcgtt gccgccgccg aaccagttcg ccctgatcaa
    12721 gtggcgggac gagatcgccg gccccgagga gctcttcgac gacatcgagc agctccgtaa
    12781 gcgccagata ccgatcgggt gggtgatcct cgacaacccc tgggagaagg gggcgttcgc
    12841 gagcggttgc tacggcgcgc tcgagttcga tccgcagcgc tacccgaatc cgaagcaaat
    12901 gatctcccgt attcacgacc tcggcgtccg cttcatgctc tgggtctcgc cccaggcccc
    12961 gaacaaggac ggctgcccgc cgccgcccta ccaggccggc cacttcgtcg gcgacgacga
    13021 gatcttcgtc gtcgatctga ccctccccga cgcgcggagc gagtaccggc ggcgactcga
    13081 cgcgctcgtc gccctggggg tcgacggctt caaaggtgat cgcggcgacg agctcgacct
    13141 cgaggaccag acgctgcacg cgggctcggg gctacgacag cacaaccgga tcccagtgtt
    13201 gttctcggat gcgattgcgg gtgcgctccg caaggtgcac ggcaggaact acggcacgct
    13261 gaaccgcgcc ggctacaccg gcgcgcagac gcggcttcac ggtctcttcg gactcgacct
    13321 gccgcacgac ttccgcggcc tccaggaggc ggtgcggatg tcacagaacg ccggcgtgag
    13381 cggcttcccc ttctggggct ccgacattgg aggctacgac ggcgactccg agatcacgtc
    13441 tgaggtcctc gttcgctggg cgcagttcgg cgcgctgacg cccgtcttcg aaatcggcgg
    13501 gcaggggctg acgagccggt tctgggagct cggcgacccc accgtcgccc tgtttcggcg
    13561 ggcggcgatc atgcactacg agctcttccc ctacctctac gagctcgcgc gcaatgcctc
    13621 gcggacgggc ctgccgatcg tccggccgct cggtttccgc taccccgccg acgagcaggc
    13681 ctggcgggca gaccagcagc tgctggtcgg gaacgacctg ctcgccgcgc ccgtgacctc
    13741 gggcacgcgg agtgggaacg acctcgcggg cccagcaact cgctggaagg tgtacgtgcc
    13801 cgccggaagc tgggtcgatg tccatcgagg aacgtcggcg cggggcccgc tcacgctcac
    13861 gcgctcgacg ccgatctccg agctgccgct gtacctccgc gcaggcgcag caatcccctt
    13921 caacagccgc tctccggcgg tgtgggcgaa gccgtggcgg gtcaacgacc tgaaccgggc
    13981 cggccgggcg ggatggctct acgcaccagg aacgagcccg acccgtgcgg ccgggggccg
    14041 cgcggggacg ttcgtggcgg cgcgcgaggg ccgaacggtc gccctccgag tcgaagccgg
    14101 gcagcgcgag acgcaggtca tcgtcctcgc acgagcccgc ccctgccggg tcgcgatcgg
    14161 gggacggccc gtcgctgagg ccaatgccgc gacgctgcgc agccgttctt cgggctggac
    14221 gttccggcgc ggctcgttcg gcgggatcac gctcaagctg accgcgccgg cgtcggccgc
    14281 cacggtcacc ttttgcgatt gaacgacgca gaaaacggct cgaatcggct cggcgggaca
    14341 tgtcagggga accagtccac gcccggtagg cgcgcagaag ccgtctgatc tgctacgatc
    14401 gcactcagat tttgtatctc actccgagtg actcgaggaa ggagcgaaca ccacactatg
    14461 gcgaaaacca tcggtatcga cctgggaacg accaattccg cgatggccgt gctggagggc
    14521 ggcgagccga cggtcatccc gaacgcagag ggcgggcgca cgaccccgtc cgtcgtcgcg
    14581 ttcacgaatg acggtcagcg gctcgtcggg gcgcccgcca ggcgccagca ggtcaccaac
    14641 ccggagaaca cgattttctc gatcaagcgc ttcgtggggc ggaagtggga cgaggtgtcc
    14701 gaggagatga cgatcgtccc gtacaaggtc gtgaagggcg agaacggcga cgcgcgcgtc
    14761 gaggcgaacg agcgcgagta cgcaccgccc gagatctcgg ccatgatcct gcagaagctg
    14821 aaggccgacg cggaggcgta cctcggggag ccggtgaccg acgcggtggt caccgtcccg
    14881 gcctacttca acaacgcgca gcgggaggcc acaaaggacg caggcaagat cgccggtctg
    14941 aacgtgcagc gcatcatcaa cgagcccacc gctgcggcgc tcgcgtacgg tctcgacaag
    15001 gaaggcgccg accagacgat cctcgtcttc gacctcggcg gtggcacgtt cgacgtctcg
    15061 gtgctcgagc tcggcgacgg cgtgttcgag gtcaagtcca cgaacggcga caaccacctc
    15121 ggcggcgaca acttcgacaa ggcgctcgtc gactggatga ccgcggagtt caagaagcac
    15181 caggggatcg acctcagcca ggacaagatg gcgctgcagc ggctctacga ggcggccgag
    15241 aaggccaaga tcgagctctc ctccacgatg acgacgcaga tcaatctgcc gttcatcacg
    15301 gcgacgcagg aggggccgaa gcacctcgac ctgccgctca cgcgggccaa gctcaacgag
    15361 ctgacgcagg agctcctgca gcggacggtc gagccgacga agcaggcgct cgccgatgcg
    15421 gagctctcgg ccggcgacat cgaacacgtc gtgctcgtcg gcggcatgac ccgcatgccc
    15481 gccgtccagg agaaggtcaa ggagctgatc ggcaaggagc cgcacaaggg cgtcaacccc
    15541 gacgaggtcg tggccgtcgg cgcggcgatc cagggcggcg tgctgaaggg cgaggtgaag
    15601 gacgtgctgc tgctggacgt gactccgctc tcgctcggga tcgagaccaa aggcggcgtc
    15661 ttcacgcagc tgatcgagcg caataccacc attcccacgc gcaaggccga gaccttctcg
    15721 acggctgagg acaaccagcc gtccgtggag atccacgtcc tgcagggcga gtccgagatg
    15781 gccgccttca acaagacgct cggcaagttc cagctcgtcg gcatcccccc ggcgccacgc
    15841 ggcatgcccc aggtcgaggt gtcgttcgac atcgacgcga acgggatcat ccacgtgacc
    15901 gccaaggatc tcggcaccgg caacgagcag cagatccgca tcgaaggcgg ctcgggcctg
    15961 tccgaggagg aggtcaaccg aatggtgacc gaagccgagg cccacgccgg cgaggcgcac
    16021 aagctgagag agctcgccga cgccaggaac ctcgccgaga cgctcgccta ccagaccgag
    16081 aagtctctga acgagcatcg ggacaagatc gaggaggccg aggccgggac gatcgagggg
    16141 cggatcatgg agctccggca ggcgctcgag gggagcgacc tcgctgacat ccagtcgaag
    16201 acccaggcgt tgcaagaggc ggcccagccg ttggcaacgg ccgtctacgc ggaggcgagc
    16261 cagcaggctg cgtccggagc cggcaacggc ggcacgaccg aggacgaggt ggtcgaggac
    16321 gccgactatg aggtcatcga cgaggacgag gcggcgacga agtcgtgacc gaggatcgcc
    16381 tcgcctcgga agagacggtg gaggaggatt ctgtcgaccc ggcaggagcc tcctccggcg
    16441 aagctgcact cgacgagcgg atggcagagg tcgagggtca gcgcgacgaa tacctggccg
    16501 acctgcagcg ccttgctgcg gacttcgaca actaccgcaa gcgcgtggcc cgggagcagg
    16561 cggcggtcgt cgcacgagca cacgagcgcc tggtcggtca cttgctgccg gtgctcgacg
    16621 atctcgagcg ggcgctcgag gcggccgagg cacacgacga ggcgcaggtg atcgaggggg
    16681 ttcggctcgt ccgcgagtcc ctcatcgcgg cgctcgagcg cgagggcctc gaggagatcg
    16741 gcgcggacgg caagttcgac ccgcatgtcc acgaggcgat gctcactcag cctgcccccg
    16801 acgtggagca gggcgacgtc gtccaagtgc tccagaaggg ctatcggctc ggcgaccacg
    16861 tgctgcggcc tgcccgggtg gtcgtcgcgg agtgagccga tgaccgccgt gaaggatccc
    16921 tacgacgtcc tcggcgtctc gaaggctgca tccgacgagg agatcaagaa ggcgtaccgc
    16981 aagctcgccc gtgagagcca tcccgaccgg aacccgggag acgaggcggc cgagagccgc
    17041 ttcaaggaca tccagacggc gtacgacctg ctctcggatg cagagaggcg gaaggagtac
    17101 gacgcctacg gcgccgccgg gccgggcgcc gggccgttcg gcccgggcgg ctatcggaac
    17161 gtgcgcgtcg agggaaccga cttcgacctc ggggacctct tcggcggaat cttcggacgc
    17221 ggcgcgcggg cgcaggcccg cccgcagccg gagcgcggcg tcgatctcga gtcgcacgtg
    17281 cgtctgtcgt tcgaggacgc tctgaagggc gtgcaagtcc gtgttcctgt cgaggtcgac
    17341 accgcctgca gcgtctgcgg cggaaccggc gccgagcctg gaaccgcacc gcgcacctgt
    17401 cccgactgcg acggcagcgg cgtccaggcc gacagtcacg gcctcttcgc gctctcgcag
    17461 ccgtgccggc gctgccgggg caacggcacg atcgtcgaca agccctgccg cagctgccgg
    17521 ggcagcggga gcgagcggtc gacgaagcgc tactccgtga agattccccc gggcgccaag
    17581 gacggtaccc gcgttcgcct caagggcaag ggcgagccgg gccggaacgg cggcccgccc
    17641 ggcgatgtgt tcgtcgtcgt cgaggtcgag gcgtccccgc tgtacgagcg gcgcgacgcc
    17701 gacctgatcc tcgacgtgcc ggtgacgtat cccgaggcgg cactcggggc gtcggtggag
    17761 atcccgaccc cgtacggttc ggtcgccctc aaggttccgg ccggctccga gaacggaaag
    17821 ctgctccgcg tcaagaaccg cggcgcgccc aagttgaagg ggaacggtcg cggcgacctg
    17881 ctggcccggc tgaggatcac ggtgccgcgc aagctctcga aggccgagaa ggaggcactc
    17941 gagggctacc agaagagctc gcgggaacga ccgcgggagc acttcacgga ggtgcggtag
    18001 ccgatgcctg accgcccgcg ctacatgatc tccgtcgctg ccgagctcgt cgacatgcat
    18061 ccgcagacgc tgcggatgta cgagagcaag gggctgatcg cgccggcacg aacgcccggc
    18121 ggcacccgcc tctactcgga gaaggacctc gagcggctgc ggctgatcca gcggctgact
    18181 accgagctgg gcctcaacct cgccggcgtc gagcacgtgc tccgcctcga ggacgagctg
    18241 cggcggctgc gcttgcgcat gcagcggctc gagcgcgagc tgcgccagga gatcaccgag
    18301 gtgcaccgga gctaccggcg cgaggtcgtc ctctgggagg cacccaagcc cgcaacgagg
    18361 cgttgaagac aacgaaagcg agaaacgagg gcaagtaaca gtctgttact tgcgaggagt
    18421 gaggatggat ttcaacaagc tgacgatcaa gtccggggaa gccgtcgccg gcgctcagga
    18481 gcttgcgcgg cggctgggca acccggagct gcaccccgag cacctcacga tcgcgctgct
    18541 cgatcaggag ctcccgcgca cgctcgtcga gcgcaccggc gcgagccctg actcgctgcg
    18601 cgcggaggcc gaggccgcgc tgcgcggcaa gccttcggtg agcggcggca acgtgcagcc
    18661 gcaggtgtcc gtgccgttca ggcgcgtgct cgacggcgcc ttcgagtgga tgaagaagct
    18721 ggaggacgac ttcgtctcgg tcgagcatct gctgctcgcg ctcggtctcg tcgccgacga
    18781 cgcggtgctt aacgtgctca aggaggtccg cggcggccag cgggtcacct cgcaagaccc
    18841 cgagggcggc tatcaggcgc tcgagaagta cggccgcgac ctgaccacgc tggcagaggc
    18901 cgggaagctc gatcctgtga tcggtcgcga cgaggagatc cgacgcacga tccaggtgct
    18961 ctcccggcgg acgaagaaca acccggtcct gatcggcgag ccgggcgtcg gcaagaccgc
    19021 catcgccgag gggctcgcgc agcgaatcgt ggccggcgac gtgccggaag ggctgaaggg
    19081 aaagcgcgtc tgggcgctcg acgtcggcgc actcctcgcc ggggcgaagt accggggcga
    19141 gttcgaggag cgcctcaagg cggttctgca agagatcact gcggccgagg gcgagatcgt
    19201 tctcttcatc gacgagctgc acaccatcgt cggcgcgggc gcggccgagg gggcggtcga
    19261 cgccgccaac atgctgaagc cgctgctcgc gcgtggcgag ctgcgcgcga tcggcgcccc
    19321 cacgctggac gagtaccgca agcacctcga gaaggacgca gcgctcgagc gccgtttcca
    19381 gccggtcttc gtcggcgagc cctcggtgca ggacaccatc tcgatcctgc gcggcctgaa
    19441 ggagcgctac gaggtgc
//
LOCUS       DBNGNK010000228         4922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1833823,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000228 DBNGNK010000000
VERSION     DBNGNK010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1833823"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(344..417)
                     /locus_tag="AC150V_06765"
     tRNA            complement(344..417)
                     /locus_tag="AC150V_06765"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Gly,seq:gcc)
     gene            488..1084
                     /locus_tag="AC150V_06770"
     CDS             488..1084
                     /locus_tag="AC150V_06770"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF013263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR3762661.1"
                     /translation="MNAVDRIRAERLVAVLRRVPDVDAVVEQLAAAGVGVVEVTMDDP
                     GAAAAIERIRRRGDVSVVAGTVRSIEDVDAAAAAGAEACVSPVFAGAVTARCHQLGMP
                     AIPGALTPTEIEAAWHAGAAMVKLFPGSAGGPQYVRDVLAPLPDVPLLVTGGVGADNA
                     VEFLRAGAAAVGVGSSLTGAADIAEAARAVIALVQTAE"
     gene            1311..3002
                     /gene="leuA"
                     /locus_tag="AC150V_06775"
     CDS             1311..3002
                     /gene="leuA"
                     /locus_tag="AC150V_06775"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR3762662.1"
                     /translation="MPFQKYRPYETVELPDRTWPDRVLERAPIWCSTDLRDGNQALVN
                     PMNTARKRRFFDLLVRLGVKEIEVGFPSASKADFDFTRALVEEDVIPADTTIAVLTQA
                     RPELIERSFAAIDGARRAIVHLYNSTSVTQRRVVFRLDRDGITDLAVRGARLCRALAA
                     DAGPEIVFEYSPESFHHTELDYALEICEAVAAEWGPAPDAKMIVNLPTTVEQFPPNVY
                     ADRIEWFCRAFSQRDAAIVSVHPHNDRGTAVATAEMGLMAGAERVEGTLFGNGERTGN
                     VDLITIALNLLTQGVDPQLDLSQIDETRAIVEECNELGIHPRHPYVGELVYTAFSGSH
                     QDAIKKGMYAQERSGSELWDVPYLPIDPMDVGRTYEAIIRVNSQSGKGGVAYLMETEH
                     HLDLPRGLQVDFAQKVQAITDARGGELVAGELMQTFRTHYLDHVAPYELVAYTHSSEG
                     DSDQIAARLLVEGEERIIEGEGNGPIAALVDAISRDLDIGVHVRDYHEHAMSSGEDAT
                     AAAYVESDVDGEVVWGVGIHPSIVTASLGAVLNAVNRSLALRAAHEEAASAFDAA"
     gene            3025..3966
                     /gene="iolC"
                     /locus_tag="AC150V_06780"
     CDS             3025..3966
                     /gene="iolC"
                     /locus_tag="AC150V_06780"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HIR3762663.1"
                     /translation="MPDLDVLTMGRVSIDLYPEQLGVHLPEVRTFRKMLGGSPTNVAV
                     AAARFGRRSAVITKVGADGFGDAVRRALGGFGVDDSFVGTHATLRTPLAFCEIFPPDH
                     FPLLFYREPTAPDMTLAADELDLDAVAAADLFWMSGTGLSAEPSRSATLAALRSRARR
                     AHTVFDLDWRPTFWSDDAPPVYEEALGLATVAVGNQEEAAVVVGDLEPLAAARALLER
                     GVALAVVKRGPAGVLAASEGEVVEVPPIAVDVVNGLGAGDAFGGALCHGLLAGWPLER
                     TVRFANAAGALVAARLGCADDMPDANEVDELLARAGT"
     gene            3963..>4922
                     /gene="nagA"
                     /locus_tag="AC150V_06785"
     CDS             3963..>4922
                     /gene="nagA"
                     /locus_tag="AC150V_06785"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR3762664.1"
                     /translation="MKLGVAAAVVDGELVRGDAEVVDGELVSVGRAGRGQGLVVPGLV
                     DLQVNGYDGVDVLTAEPEELAAMGRSLARAGVLWYQPTLITAPAERTRSALDAIGSTI
                     GVGGGARILGAHLEGPFLSAARPGAHDVDLLRDPNKDLLASYLLSGCPVTMVTVAPEL
                     PGADEVIDALVERGVVVSLGHTDAGVVAAHAAFDLGARAVTHLYNAMRPFSHRDPGTV
                     GVALSREDVFVQLIADGVHVSPEAILTAWRAARSRLVLVSDAISAAGLGDGTFHVGDL
                     EVEVERGVARTADGTLAGAVRSLEWGMRVLAEHGVPIPEAVDAV"
BASE COUNT          726 a         1701 c         1735 g          760 t
ORIGIN      
        1 atcctcggga tcaacgccgt tgccctctgg tacgcactca gagagaacgg attccgccag
       61 cctctcacgg gagggggcgt gctcctgcga gagcactgac atctcctgct ttccgccgcc
      121 tggtcacgat ccgcagaacg tgccgtggct ctagctcttg tgcagcgccc aaacgccgta
      181 tccaactgat caggaggccg agcgccagaa acctcgccgt cgagcgcgat ctcgcacgct
      241 ccgtgcgcga aggagacggg agccgctcga caccaagcgg acacgatccg ggacttgtcg
      301 cccttacggc aaacaaaaac ccccggttta cgggggtttc gcaagcggat gatgggactc
      361 gaacccacga ccttctgcat ggcaagcaga cgctctagcc aactgagcta catccgcgtg
      421 gaagccggag tatagccccg cgccgacggc gcggctcccg gccgggccgc ctgtatcgtc
      481 ggcgggtgtg aacgccgtcg accgcatccg cgccgagcgc ctcgtcgcgg tgctgcggcg
      541 tgtccccgac gtcgacgccg tcgtcgagca actcgctgct gccggcgtcg gtgtggtgga
      601 ggtgacaatg gacgatcccg gcgcggcggc cgcgatcgag cggatccgtc ggcggggtga
      661 cgtgtcggtc gtcgcgggga cggtgcgttc aatcgaggac gtcgacgctg cggcggctgc
      721 cggggccgag gcatgcgtca gcccggtgtt cgccggcgct gtgacagcgc gctgccacca
      781 gctcggcatg cccgcgatcc cgggcgccct gacgccgacg gagatcgagg cggcctggca
      841 cgcaggggcg gcgatggtga agctcttccc cggatccgcc ggtggaccgc agtacgttcg
      901 cgacgtcctc gcgccgcttc cggacgtccc actgctcgtg acgggcggcg tcggagccga
      961 caacgccgtc gagttcctcc gcgcgggtgc agcagccgtc ggggtgggct cgtcgctcac
     1021 gggtgcggcg gacatcgccg aggccgcccg cgctgtcatc gccttggtgc aaaccgccga
     1081 atagcagcgc gatacttggc gttgaagccg cgcctgggct atcgtgcagg cgagatggca
     1141 ggcagcatcg atcggatcac ggcgcccggc gcgacggcgc tgctcggcct tgccctcctc
     1201 cttccccgct aggggaagag agcctgtcga catacccgtt cccgcgctcc cgcatcgagg
     1261 ggaggagtcc gttcacgcac gaaccagacg aggagcttga gccgatgacc atgccgttcc
     1321 agaaataccg accgtacgag accgtcgaac tgcccgaccg cacgtggccc gatcgagtgc
     1381 tcgagcgcgc gccgatctgg tgctcgaccg acctccgcga cggcaaccag gcgctcgtga
     1441 acccgatgaa tacggcgcgc aagcgacgct tcttcgatct gttggtgcgc ctcggggtca
     1501 aggagatcga ggtcggcttc ccatccgcgt cgaaggcgga cttcgacttc acccgcgcgc
     1561 tcgtcgagga ggacgtcata ccggccgaca cgacgatcgc agtgctgacc caggcgcggc
     1621 cggagctgat cgagcgctcc ttcgccgcga tcgacggcgc tcgccgcgcg atcgtccatc
     1681 tctacaactc gacctcggtc acgcagcgcc gagtcgtgtt ccggctcgac cgggacggca
     1741 tcaccgacct cgccgttcgc ggcgcgcgcc tctgccgcgc cctcgccgcg gatgcggggc
     1801 ccgagatcgt cttcgagtac tcgccggaga gctttcacca caccgagctc gactacgccc
     1861 tcgagatctg tgaggccgtt gccgccgagt ggggcccggc ccctgacgca aagatgatcg
     1921 tcaacctccc gacgaccgtg gagcaattcc cgccgaacgt ctacgccgat cggatcgagt
     1981 ggttctgtcg cgccttctcg cagcgcgatg cggcgatcgt ctcggtgcat ccgcacaacg
     2041 atcgcggcac ggcggtcgcg accgccgaaa tgggcctgat ggccggcgcc gagagggtag
     2101 aggggacgct gttcggcaac ggcgagcgca ccggcaacgt cgacctcatc acgatcgccc
     2161 tgaacctgtt gacgcagggc gttgatcccc agctcgatct ctctcagatc gacgagaccc
     2221 gcgcgatcgt cgaggagtgc aacgagctgg gcatccatcc caggcatccg tatgtcggag
     2281 agctcgtcta cacggccttc tcgggctcgc accaggacgc gatcaagaag ggcatgtacg
     2341 cacaggagcg gtcaggctca gagctgtggg acgtgccgta cctgccgatc gacccgatgg
     2401 acgtcgggcg cacctacgag gcgatcatcc gtgtcaactc ccagtccggg aagggcggcg
     2461 tcgcgtacct gatggagacc gagcaccacc tcgatctgcc ccgtggcctc caggtcgact
     2521 tcgcccagaa ggtgcaggcg atcaccgacg cgcgcggcgg tgagctcgtc gccggagagt
     2581 tgatgcagac cttccgcacg cactacctcg atcacgtggc gccgtacgag ctcgtcgcct
     2641 acacgcactc gagcgagggc gactccgacc agatcgcggc ccgcctgctc gtcgaaggcg
     2701 aggagcgaat catcgaagga gagggcaacg gcccgatcgc ggcgctcgtc gacgcgatct
     2761 cacgtgacct tgacatcggc gtgcatgtgc gcgactacca cgagcacgcg atgtcgagtg
     2821 gcgaggacgc gacggcggct gcgtacgtcg agagcgacgt cgacggcgag gtcgtctggg
     2881 gagtcggtat tcacccgtcg atcgtcaccg cctcgctcgg cgccgtgctc aacgcggtca
     2941 acaggtcgct cgcgcttcgt gccgcgcacg aggaggccgc ctcggcgttc gacgccgctt
     3001 agacgctcct cgtagagtgg cgtcgtgccc gatctcgacg tgctcacgat gggccgggtg
     3061 agcatcgacc tctacccgga acagctcggt gtgcacctgc ccgaggttcg aacgttccgc
     3121 aagatgctcg gtggcagccc gacgaacgtc gccgttgcgg cggcccgctt cgggcggcgg
     3181 agcgccgtca tcacgaaggt cggggcggac gggttcggcg acgcggtccg gcgcgcgctc
     3241 ggcggcttcg gggtcgacga ctcgttcgtc ggcacccacg ccacgctgcg gacgccgctg
     3301 gccttctgcg agatcttccc gccggatcac ttcccgctcc tcttctaccg cgagcctacg
     3361 gcgcctgaca tgacgctcgc cgcggacgag ctcgacctcg acgcggtcgc cgcagcggac
     3421 ctcttctgga tgtcgggcac cgggctgtcc gccgagccga gccggtcggc gacgctggcg
     3481 gcgctgcgat cgcgggcaag gcgggcccat acggtgttcg acctcgactg gcggcccacc
     3541 ttctggtccg acgacgctcc ccccgtctac gaggaggcgc tcggcctggc gacggtcgcc
     3601 gtcggcaacc aggaggaagc ggcggtcgtc gtcggcgatc tggagccgct cgcggccgcg
     3661 cgcgccctgc tcgaacgcgg cgtagccctc gccgtcgtga agcgcggccc ggccggcgtg
     3721 ctcgccgcca gcgagggcga ggtggtcgag gtgccgccga tcgccgtcga tgtcgtcaac
     3781 ggcctggggg ccggcgatgc gttcggcggc gcgctgtgcc acggcctgct cgcgggctgg
     3841 ccgctcgagc ggacggttcg gttcgcgaac gccgcgggcg cgctggtcgc ggcacgactc
     3901 gggtgcgccg acgacatgcc cgacgcgaac gaggtggacg agcttctggc gcgggccggc
     3961 acatgaagct cggcgtcgca gcggccgtcg tcgacggcga gctcgtccgc ggcgacgcgg
     4021 aggtggtgga cggcgagctc gtgagcgtgg gccgggcagg ccgtgggcag gggctggtcg
     4081 ttcccgggct cgtcgatctc caggtgaacg ggtacgacgg cgtcgacgtg ctgacggctg
     4141 agccggagga gctcgccgcc atgggacgct ccctcgctcg cgccggcgtg ctctggtacc
     4201 agcccaccct gatcacggcg ccggccgagc ggacgcgatc ggcactcgat gcgatcggca
     4261 gcacgatcgg agtgggggga ggcgcgcgga tcctcggcgc gcatctcgag gggccgttcc
     4321 tgtctgccgc gcggccgggc gcccacgacg tggacttgct gcgtgacccg aacaaggatt
     4381 tgctcgcctc ctacctgctc tcagggtgcc ccgtgacgat ggtgaccgtc gcacccgagc
     4441 tgcccggagc ggacgaggtc atcgacgcgc ttgtggagcg gggcgtcgtc gtctcgctcg
     4501 ggcacacgga cgcgggcgtc gtcgccgccc acgcggcatt cgatctcggt gcccgcgcgg
     4561 tgacgcatct gtacaacgcg atgcgaccgt tctcccatcg cgatcccggg acggtgggcg
     4621 tcgcgctctc acgtgaggac gtcttcgtcc agttgatcgc ggacggcgtg cacgtctcgc
     4681 cggaggcgat cctcacggcg tggcgtgctg cgagaagtcg cctcgtgctc gtctccgacg
     4741 cgatctcggc ggccggactc ggcgacggca cgtttcacgt gggcgacctc gaggtggagg
     4801 tcgagagagg cgtcgcacgg acggcggacg gtacgctcgc gggcgccgtg cgctcgctgg
     4861 agtgggggat gcgggtgctc gccgagcacg gcgtgccgat ccccgaggcc gtcgacgccg
     4921 tc
//
LOCUS       DBNGNK010000229         3304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_902415,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000229 DBNGNK010000000
VERSION     DBNGNK010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_902415"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..2517
                     /locus_tag="AC150V_06790"
     CDS             58..2517
                     /locus_tag="AC150V_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HIR3762665.1"
                     /translation="MRLRRRREAPPRRRRIRKLRLLALLMVLLALATVAFGLGLVRAV
                     ASEIPKLDPVNQQAAGEIDSVIYASNGRSVLAVLRGSESRVLVESIDDIAPIMRQAIV
                     AVEDKRFYEHKGVDLRGIGRALWEDIRSRKVVEGGSTITQQFVKNAYIRNEQSIGRKV
                     REAALAWQLEQRWSKDRILAAYLNTIYFGNGAYGIQQAAKTYFGKGSTKLLLHEAALL
                     AGIPANPGRYDPVTRPRAAKARRQFVLTKMTNQGLITPGAKRWANKQPLPNPENVGLP
                     GTRGPAPYFVNYVSDQLVQEFGAGRVFGGGLHVTTTIDLKLQELAREAIERVLESPSG
                     PNAALVAIDPRTGAVQAMFGGQSFRKSQFNLATQAERQPGSSFKPIVLASALRQGISP
                     VTQFQSKPVTIDAGDRVWRVRNYEDVYLGNVDLANALVKSDNAVYAQLTKVVTPKKIV
                     ETATALGISSELNPYFSIGLGALAVNPLDMTRAYATLANDGARIDGALMGDRPRVIEN
                     VEWVKSGRVRENAPLERTILTEAEAHTLTSILQRVVTSGTGRRAALPDRTSAGKTGTT
                     DNYGDAWFVGYTPQLAVAVWVGYPDELRPMLTEFGGDPVTGGTLPAQIWNEFMTLALA
                     ELDEEPEPFPAAPWLPAEERRIVWRGGGYKLDNGYCRNTRRIAYVADRGPQEVADCKP
                     NEVSVPRVVGQAVDEARQNLAATPLEVSVVYAPAKPATKPGMVVRQDPVSGFLSAYDT
                     VRLTVTKARYGLVPNLIGSSVEDAREQLLRRNLKVRVSWGKGPPGTVVRQSPEAGVAA
                     DPGYAIKLVVARGTRTATASP"
     gene            complement(2468..3061)
                     /locus_tag="AC150V_06795"
     CDS             complement(2468..3061)
                     /locus_tag="AC150V_06795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR3762666.1"
                     /translation="MALLQETGLLDRAFFRRDVLEVARDLVGARLLVDGVGGTIAEVE
                     AYGPDDPASHSYRGRTARNVAMFGPAGHAYVYRSYGIHWCFNAVCDADGVGAAVLVRA
                     LVPEHGVDEMRRRRGVDDERLLCSGPGRLTQALGIVGACDGLPLDRSPFTLRAAPTRA
                     DVDTTPRIGISRATDLRRRYVLTGSRSRFASRGRPRA"
     gene            complement(3067..>3304)
                     /locus_tag="AC150V_06800"
     CDS             complement(3067..>3304)
                     /locus_tag="AC150V_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762667.1"
                     /translation="WVEGLEDALIASRTERTILAEMGQEGGRDDQLLDDALDLAERLA
                     AAADEGRVDLGDLGRRARTVLAATSRSDESDSLF"
BASE COUNT          550 a         1139 c         1147 g          468 t
ORIGIN      
        1 ggcctgagca gaacgtcgcg gccgggaaac cgcggaaaag ggctagaatt gcgtggtttg
       61 cggcttcgaa ggagacggga ggcgccgccc cgccggcgcc ggattcggaa gctgcggctg
      121 ctggcgctct tgatggtgct gttggcgctg gcaaccgtgg cgttcggcct cgggctcgtt
      181 cgcgcggtgg cgagcgagat cccgaagctc gaccccgtca accagcaggc ggccggtgag
      241 atcgacagcg tcatctacgc ctccaacggg cgctcggtcc tcgcggtgct ccgcggctcg
      301 gagtcccgcg tcctcgtcga atcgatcgac gacatcgccc cgatcatgcg tcaggcgatc
      361 gtcgcagtgg aggacaagcg cttctacgag cacaagggcg tcgacctccg cggcatcggc
      421 cgtgcgttgt gggaggacat ccggagccgg aaggtcgtgg aaggcggctc gacgatcacc
      481 cagcagttcg tcaagaacgc ctacatccgc aacgagcagt ccatcggccg gaaggtgcgc
      541 gaggcggcgc tcgcgtggca gctcgagcag cgctggtcga aggaccggat tctggcggct
      601 tacttgaaca ccatctactt cgggaacggc gcctacggca ttcagcaggc ggcgaagacg
      661 tacttcggaa agggctcgac caagctcttg ctccacgaag ccgcgctcct tgcgggcatt
      721 cccgccaacc cgggtcgcta cgacccggtc acgcgccccc gcgccgccaa ggcgcggcgg
      781 caattcgtgt tgacgaagat gacgaaccag gggctgatca cacccggcgc gaagcgctgg
      841 gccaacaagc agccgctccc gaacccggag aacgtcggcc tccccggaac gcgcgggccg
      901 gcgccgtact tcgtcaacta cgtcagcgat cagctcgtgc aggagttcgg tgcgggacgc
      961 gtcttcggcg gcggcctcca tgtcacgacg acgatcgacc tcaagctcca ggagctggca
     1021 cgcgaggcga tcgagcgggt gctggagagc ccctccggcc ccaacgccgc cctcgtcgcg
     1081 atcgacccgc gaaccggcgc cgtacaggcg atgttcggcg gtcagagctt ccgcaagagc
     1141 cagttcaatc tcgccacgca ggccgaacgg cagccgggtt cctccttcaa gccgatcgtg
     1201 ctcgcctcgg cccttcgcca ggggatctcc cccgtcactc agttccagtc caagcccgtg
     1261 acgatcgatg ccggcgatcg ggtctggcga gtgcggaact acgaggacgt ctatctcggc
     1321 aatgtcgatc tcgcgaacgc gctggtgaag tcggacaacg ccgtctacgc acagctgacg
     1381 aaagtcgtca cgccgaagaa gatcgtcgag acggcgaccg cgctcgggat cagcagcgag
     1441 ctcaacccgt acttctcgat cggcctcggc gctctggccg tgaatccgct cgacatgacc
     1501 cgcgcgtatg cgacgctcgc caacgacgga gcgcggatcg acggagccct gatgggcgac
     1561 aggccccgcg tcatcgagaa cgtcgagtgg gtgaagagcg ggcgtgtgcg ggagaacgcg
     1621 ccgctcgagc gcacgatctt gaccgaggcc gaggcgcata cgctgacgtc gatcctccag
     1681 cgggtcgtca cctccggcac cggacggcgg gccgcgctgc ctgaccgtac gtcggcaggc
     1741 aagaccggca ccaccgacaa ctacggcgac gcgtggttcg tcgggtacac gcctcagctc
     1801 gccgttgcgg tctgggtcgg atacccggac gagcttcggc cgatgctgac cgagttcgga
     1861 ggcgacccgg tcacgggcgg caccctgccc gcccagatct ggaacgagtt catgacgctc
     1921 gcccttgcgg agctcgacga ggagccggag cccttcccgg cggctccgtg gcttcccgcc
     1981 gaagagcgcc ggatcgtctg gcgcggcggc ggctacaagc tcgacaacgg ctactgccgg
     2041 aacacacgcc ggatcgcata tgtcgccgac cgcgggccac aggaggtcgc cgactgcaag
     2101 cccaacgagg tctcggtgcc gcgggtcgtc ggccaggccg tcgacgaagc acggcagaac
     2161 ctcgcggcca cgccgctcga ggtctcggtc gtctatgcac cggcgaagcc tgcgacgaag
     2221 ccgggaatgg tcgtccggca ggacccggtc agcggcttcc tgtccgcgta cgacacggtc
     2281 cggctcacgg tgacgaaggc ccgctacggg ctcgtcccga acctgatcgg ctcgagcgtc
     2341 gaggatgctc gcgagcagct cttgcgacgg aacctcaagg tgcgggtgtc gtgggggaag
     2401 ggcccgccgg gaacggtggt gaggcagagc ccggaagcgg gcgtcgccgc cgaccccggc
     2461 tacgcgatca agctcgtggt cgcccgcggg acgcgaaccg cgaccgcgag cccgtgagca
     2521 cgtagcggcg gcgcaggtcg gtcgcccggc tgatcccgat acgcggcgtc gtgtcgacat
     2581 cggcgcgcgt cggcgccgca cgcaacgtga acggcgagcg atcgagcggc agcccgtcgc
     2641 atgcaccgac gatgcccagc gcctgggtca gccggccggg cccggagcag agcaggcgct
     2701 cgtcgtcgac accccggcgt cggcgcatct cgtctacccc gtgctcggga acgagtgcgc
     2761 gaacgagaac agccgctcct actccgtctg cgtcgcagac ggcgttgaag caccagtgga
     2821 tcccgtagga gcggtagacg taggcatgtc cggcggggcc gaacatcgcg acgttgcgcg
     2881 ccgtccgccc gcgatacgag tggctcgccg gatcgtccgg cccgtacgcc tcgacctcgg
     2941 cgatcgttcc gcccaccccg tcgacgagga gcctcgcgcc gacgaggtcg cgggctacct
     3001 cgagcacgtc gcgccggaag aaggcgcggt cgaggagacc ggtctcctgg agcaaggcca
     3061 cggggctcag aagagactgt cggactcgtc gctgcgggag gtcgccgcga gcaccgtgcg
     3121 tgcgcggcgg cccagatcgc cgaggtcgac gcggccctcg tcggcggccg cggcgagccg
     3181 ctcggcgagg tcgagcgcgt cgtcgagcaa ctggtcgtcg cgcccgcctt cctgccccat
     3241 ctcggcgagg atcgttcgtt cggtacgcga cgcgatgagc gcatcctcga gcccttctac
     3301 ccac
//
LOCUS       DBNGNK010000230         3149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_471959,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000230 DBNGNK010000000
VERSION     DBNGNK010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_471959"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="AC150V_06805"
     CDS             <1..367
                     /locus_tag="AC150V_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR3762668.1"
                     /translation="LLVTGYDEENARMILSVPPGVEPRRVSVDAIEHWMIYQAHPDVG
                     AVLHVHAWMEGIAATDINYPCGTQELAESVAELIDREPDPAHAVIGLRNHGITATGDS
                     LAEILDRIEPKVLRQVPMT"
     gene            complement(372..812)
                     /locus_tag="AC150V_06810"
     CDS             complement(372..812)
                     /locus_tag="AC150V_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR3762669.1"
                     /translation="MKAGVLRAGVGLVALAGVALSGYLTYVHFDEGALICTSGGGCET
                     VQESEYAELAGIPVAVLGLAAWVTVLALTIWNSALARTLTAAIALGALAFAAYLVVLQ
                     LFVIDAVCIWCMVNDLVLAPLLAILAVLRLRQDAAGHGSEPEPG"
     gene            complement(809..1558)
                     /locus_tag="AC150V_06815"
     CDS             complement(809..1558)
                     /locus_tag="AC150V_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR3762670.1"
                     /translation="MPPKPRAPKPKKKGAPESGGLGSRRNLLIAGGIAVLVAAALIIG
                     SLVLTAGDDESSAGGTAVAASDLTGIPQNGAVLGEESANVTLIVYEDAQCPVCKSYSE
                     DGFPGLVDEFVRSGQINMEFRGLSFLGPDSLKALRFMYAAGEQNKLWDFTEAIYANQG
                     GENEGWVTDDLVREIAEEVGLDVDKLFADADSDLVTQKIERAQQQASQAEVPGTPWFY
                     IKVGDDEPYEIQPTSLSPEAFRDALNAAIQG"
     gene            1662..>3149
                     /locus_tag="AC150V_06820"
     CDS             1662..>3149
                     /locus_tag="AC150V_06820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR3762671.1"
                     /translation="MAGAEEKLRALVETGIALTSELALDAVLERVVESAADLTAARYA
                     ALGVIDRDGKGLERFVAHGVTPEQHAAIGELPRGEGLLGALIRDARPLRLADLGTDPR
                     SVGFPPNHPPMRSFLGVPVLLRGVAYGNLYLTEKRGGAEFTLEDEEFAGLLAAQAAVA
                     IENARLYESELRWSSQLEALEEIAVALAGEAELPRQLELVAARLRQLLSARIVAVALA
                     RDDGTLLVHAGSGEDLAGLVLPADTKTARVLSRRRSELVDSLRDDLEVDRRLVERFDA
                     HAALIVPLLVGGRALGVLVAVDKDGPDERFDDDDLRLAESYARRAAVAVDLSQRVARD
                     ALRRAVAAQELERRRIARELHDETGQALTSILLGLSSIDPDGDKTELRNALTGVRDLA
                     VSTLQDVRRLAVELRPKALDDFGLLPALRRLGQAVSERGSLVVQVEAHLGDERLTPEA
                     ETAVYRIVQEALTNVVRHADATHVSVVLTRKNAGVSVVIEDDGRGF"
BASE COUNT          474 a         1077 c         1091 g          507 t
ORIGIN      
        1 tctcctcgtc accggctacg acgaggagaa cgcgcggatg atcctcagcg tgccgccggg
       61 cgtcgagccc cggcgcgtct cggtcgacgc gatcgagcac tggatgatct accaggctca
      121 ccccgacgtc ggcgcggtcc tgcacgtgca cgcgtggatg gagggcatcg ccgccaccga
      181 catcaactat ccgtgcggca cccaggagct ggccgagagc gtggccgagc tgatcgaccg
      241 cgagcccgac cccgcgcatg ccgtgatcgg cctgcgcaac cacggcatca ccgcgaccgg
      301 cgattccctg gcggagattc tcgaccggat cgagccgaag gtgctccgcc aggtgccgat
      361 gacctagcgg cctagccggg ctcgggctct gagccgtgcc cggccgcgtc ctgccggagc
      421 ctgaggacgg cgaggatggc gagcagcggc gcgagcacca ggtcgttgac catgcaccag
      481 atgcagacgg catcgatcac gaacagctgc agtacgacga ggtaagcggc aaacgcgagc
      541 gcgccgagcg cgatcgccgc cgtgagcgtc cgcgcgagcg ccgagttcca gatcgtgagt
      601 gccagcaccg tcacccaggc tgcgagaccg aggacggcga ccggaatgcc tgcgagttct
      661 gcatattccg actcctgcac cgtctcgcag ccgccgccgc tggtgcagat cagcgcgccc
      721 tcgtcgaagt ggacgtaggt gaggtagccg gacagggcga cgccggcgag cgccacgagc
      781 ccgacgcccg cccgcaggac tcccgctttc acccctgaat cgctgcgttc agcgcgtccc
      841 ggaatgcctc gggcgacagc gaggtcggct ggatctcgta cggctcgtcg tcgccgacct
      901 tgatgtagaa ccagggcgtc ccgggcacct ccgcctggct ggcctgctgc tgcgcgcgct
      961 cgatcttctg cgtcacgagg tcggagtcgg cgtcggcgaa gagcttgtcc acgtcgagtc
     1021 cgacttcctc ggcgatctct cgcacgaggt cgtctgtgac ccagccctcg ttctcgcctc
     1081 cctggttcgc gtatatcgcc tccgtgaagt cccagagctt gttctgctcg ccggctgcgt
     1141 acatgaagcg aagagccttg agcgagtcgg gcccgaggaa ggagagcccc cggaactcca
     1201 tgttgatctg gcccgaccgc acgaactcgt cgacgaggcc ggggaagccg tcctcgctgt
     1261 agctcttgca gaccggacac tgcgcgtcct cgtagacgat gagcgtcacg ttggccgact
     1321 cctcgccgag cactgcgccg ttctgtggga tgccggtgag gtcggaggcg gcgactgcgg
     1381 ttccaccggc tgagctctcg tcgtctccag cggtcagcac caggctgccg atgatcagtg
     1441 cggcagcgac gaggacggcg atgccgcccg cgatgaggag gttccggcgc gacccgagac
     1501 cgcccgactc gggagcgccc ttcttcttcg gcttgggtgc tcgtggctta ggaggcatgg
     1561 tcagagtgtg ccaacagagc cggaagtctg ttttaagaac gcgtttggct gacgcccccg
     1621 cggacggctc tgcgcaacgt cggcaggcca taatcggctc catggccggg gcagaggaga
     1681 agctgcgggc tcttgtcgag acggggatcg cgctcacatc ggagctcgcg ctcgatgccg
     1741 tgctcgagcg ggtggtggag tccgcggccg atctgaccgc tgcgcgctac gcggctctgg
     1801 gcgtcatcga ccgcgacggc aagggcctcg agcggttcgt tgcacacggc gtgacccctg
     1861 aacagcatgc agcgatcggc gagctgccgc gcggagaagg gctgctcggg gcgctgattc
     1921 gggatgcgcg gccgctccgc ttggccgacc tcggtaccga cccgcgctcg gtcggctttc
     1981 cccctaatca tccgccgatg cgctcgttcc tcggcgtgcc cgtcctcctc cggggcgtcg
     2041 cgtacggaaa cctctacctg accgagaagc gcggtggcgc cgagttcacg ctcgaggacg
     2101 aggagttcgc cggcctcctc gctgcgcagg ccgccgtcgc gatcgagaac gcgcgtctct
     2161 acgagtcgga gctccgctgg tcgagtcagc tggaggcgct cgaggagatc gccgtcgctc
     2221 tcgcgggcga ggccgagctg ccgcgacagc tggagctcgt ggcggcgcga cttcgccagc
     2281 tgctgtctgc gcgcatcgtc gcggtggcgc tggcacgtga cgacggaacg ctcctcgttc
     2341 acgccggcag tggggaggat cttgccgggc tggtgctgcc ggccgatacc aagaccgcac
     2401 gggttctctc gcgacgccgg agcgagctcg tcgactcgct gcgggacgat ctcgaggtgg
     2461 accggcggct ggtggagcgc ttcgacgcgc acgcggccct gatcgtgccg ctgctcgtcg
     2521 gcggccgcgc gctcggcgtc ctcgtcgccg tcgacaagga cgggcccgac gagcggttcg
     2581 acgacgacga cctgcggctc gccgagagct acgcgcggcg tgccgccgtc gctgtagacc
     2641 tttcccagcg cgtcgcccgg gacgcgctcc gccgcgcagt tgccgcgcag gagctcgagc
     2701 gccgccgcat cgcacgggag cttcacgacg agaccggcca ggctctgacg tcgattctgc
     2761 tcggcctctc ctccatcgat ccggacggtg acaagacaga gctccgaaac gcgctgacgg
     2821 gggttcgcga tcttgccgtc tcgacgctcc aggacgtgcg ccgactcgcc gtcgagctgc
     2881 gcccgaaggc cctcgacgac ttcgggttgc tccctgctct tcgacggctt ggccaggcgg
     2941 tctccgagcg cggctcattg gtcgtgcagg tagaggcgca cctcggtgac gaacgactga
     3001 cacccgaggc cgagaccgcc gtctacagga tcgtgcagga ggcgctgacg aatgtcgtgc
     3061 gccatgcgga cgcaacccac gtcagcgtcg tcctgaccag gaagaatgca ggtgtgtcgg
     3121 ttgtgatcga agacgacggc cgtggcttc
//
LOCUS       DBNGNK010000231        10782 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1046102,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000231 DBNGNK010000000
VERSION     DBNGNK010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1046102"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="AC150V_06825"
     CDS             <1..848
                     /locus_tag="AC150V_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005004716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HIR3762672.1"
                     /translation="NPHTKPLPFWEWCIGELKTAHPDLVFLSEAFTRPKVMHRLAKAG
                     FTQSYTYFAWRNTKWELTEYFKELSKEDSREYFRPNAWPNTPDILTELLQFGGRPAFV
                     SRLVLAATMAASYGIYGPAYELCEHEPREPGSEEYLDSEKYEIRSWDLDRADSLRDLI
                     GRVNTIRRENPALQQDWTLEFHEVDSDQLICYSKTTDDFENVVLVVVNLDPHHTQAGW
                     VNLSLDELGLDEQPFQVHDLLGGSRFLWQGSHNYVELDPQVLPAHIFLVRRRVRTERE
                     FDYYL"
     gene            845..4213
                     /gene="treS"
                     /locus_tag="AC150V_06830"
     CDS             845..4213
                     /gene="treS"
                     /locus_tag="AC150V_06830"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HIR3762673.1"
                     /translation="MSHATTSGRPGTESVRSHDPLWFKDAVIYQLHVRAFHDSSGNGI
                     GDFSGLTEKLDYLHDLGVTALWLLPFYPSPLRDDGYDIADFRGIHPDYGSLREMQRFL
                     RDAHRRGLRVITELVVNHTSDQHPWFQRARRAPAGSRWRDFYVWSDTPDRYRDARIIF
                     QDFEPSNWSWDPVANAYFWHRFYSHQPDLNYESPDVRRLILRTLNYWFNLGVDGLRLD
                     AVPYLYEREGTMCENLPETHDYLKELRAHVDERFENRMLLAEANQWPEDAAAYFGDGD
                     ECHMAFHFPVMPRLFMAVQMEDRYPIIDILQQTPAIPDSSQWALFLRNHDELTLEMVT
                     DEERDYMYRVYAHDRQARINLGIRRRLAPLLGNNRAKVELLYGLLFSLPGTPIVYYGD
                     EIGMGDNVYLGDRDSVRTPMQWSGDRNAGFSRANPQQLYLPVVIDPEFHFETVNVETQ
                     LANPHSLLWWMRRLIALRARHHVFGRGSLEFLTPENHKVIAFVRRDGDEMILVVANLS
                     RFAQFVELDLAEFKGLVPFELFGRTAFPPIGELPYLLTLGPHAFYWLSLEQPRDEVPG
                     VADDGHAVTISVPGRWNEVFSGKTKRAAEDSISRFMQRRRWFAGKARSVHDVSVRDVV
                     SVPIPERDPAMLVLVDVEYVEGEPEAYILPLAVAVGEPGERIAVDDPHAVVARLARGS
                     EAAVLYDALHDERFCAALLDAVAKRRRFRGSKGSVVGQPTPSLRRILADVEGQLEPDL
                     FRAEQSNSSVVFGQRLILKLFRRSEEGVNPDLELGIFLGQRAFSHAPAVAGSLEYEQS
                     GREPITLAIAHEVVANEGDAWRYTRDTVGHFYERVLTENGGLATGGGARERPLIELVG
                     EELPSVAHATSEAYLRSAELIGQRTAELHNTLASGVDTEAFAPEPFTPLYQRSVYQSM
                     RNLTRRNFRLLRRSLPVLAPRERAQAERILAAESDLLERFRAVLDRTLSGLRIRYHGD
                     FHLGQVLYTGKDFVIIDFEGEPARSLGDRKIKRSPLRDVAGMLRSFEYAGYAGLFDQV
                     ERGTVEAESDTFRLLEGWCRRWSSWASVSFLRSYLETADDHYLPGTREELHSVIDLFL
                     LEKAVYELGYELNSRPDWVRIPLAGILRLLGERP"
     gene            4210..6186
                     /gene="glgB"
                     /locus_tag="AC150V_06835"
     CDS             4210..6186
                     /gene="glgB"
                     /locus_tag="AC150V_06835"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009196374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR3762674.1"
                     /translation="MTRRAKGNDSVPPAKERRQDIVDPGEAFLGDDDLHLFNEGTHTR
                     LYEKLGAHVAALDGTAGVQFAVWAPNAERVSVVGDFNGWEAGSHPLRPRASSGIWQGF
                     VPELPSGSVYKYAIVSKTGDHRVEKADPFAFAAEIPPKTASVVWELDYEWGDGGWMKR
                     RMDANALDAPISIYEMHLGSWRRVPADGDRSLSYLELAEALPDYVRERGFTHVELIPL
                     MEHPFYGSWGYQTTGFFAPTSRYGTPQDFMALVDALHQAGIGVILDWVPSHFPDDEHG
                     LAYFDGSHLYEHGDPREGRHPDWGSLIFNYGRHEVRSFLLSSALYWLDRYHADGLRVD
                     AVASMLYRDYSRKEGEWIPNEHGGRENLEAIGFLRQLNEEVYGQYPDVQTIAEESTAW
                     PMVSRPTYLGGLGFGLKWDMGWMHDTLHYMARDPVFRPHHHNELTFRAIYASNENFLL
                     PLSHDEVVHGKGSLLGRMPGDEWQKLANLRLLYGYQWAQPGKKLLFMGGEFAQGSEWN
                     HDANLEWELVERPTHRGVQRWIDDLNRVHREEAALHELDCDPAGFDWVDANDSAASVV
                     AFLRRSRTGEEVLVICNFTPVPRHNYRVGVPTGGVWEELLNSDAHDYGGSGQGNLGGI
                     EATPVPYHGRRKSLVLTLPPLAALFVKPQRSGDA"
     gene            6183..7424
                     /locus_tag="AC150V_06840"
     CDS             6183..7424
                     /locus_tag="AC150V_06840"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR3762675.1"
                     /translation="MRPRWLDLPRSSGILLHPTSLPGGRLGAGARSFVDWLAAAGQSW
                     WQVLPLGPPDDHCSPYKPTSAFAGSPSLLAEPDARVDAAEVDRFVEKHSYWASDWAEY
                     AGDGALEDQVRFEREWSELRAYAAARGVRLIGDIPIYVAEGSADHVSHPELFQDDVVA
                     GVPPDDFSATGQLWGNPLYDWPALRRTGYEWWIERFHRVLKLVDLARVDHFRGFVAYW
                     AVAAGSPTAMHGRWRRGPGMALFREAEAALGPLPLIAEDLGVITPPVVRLRDELGVPG
                     MVILQYAFGDDGRSPHVLANHPECAVVFTGTHDHAPIAAWWTEEAHSGERDRADRAAA
                     ALGISDPESHWRLLRLAFSSRARLAIAPMQDVLGLGREARMNTPATDAGNWGWQLPAG
                     QLTSELADRLRTSTEAAGRKA"
     gene            complement(7489..8103)
                     /locus_tag="AC150V_06845"
     CDS             complement(7489..8103)
                     /locus_tag="AC150V_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR3762676.1"
                     /translation="MKTASRWFLPDSPDVLGMLKAQIEVTKRGMDALVEWANGDASAA
                     EVVRATEHEADEHKRALQRGLVEAFTTPYDAEDIYTLSRGIDWILNLSKDAVRESEVM
                     DCSPDAPIAEMAALLRDAVGSLGEAVAGLQARDDRATKAADAAIKANRRLERVYRRAM
                     AELLEVDDFRIVMSRRELYRRFSRISEVVSDVGERFWYAVIKES"
     gene            complement(8100..9110)
                     /locus_tag="AC150V_06850"
     CDS             complement(8100..9110)
                     /locus_tag="AC150V_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005568777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR3762677.1"
                     /translation="MDTSLVVAVALAFAFALTNGFHGASNSIAALVATRAARPSRAVI
                     LAAVFNFLGPLLVGSAVANTIATIVDVPANETVTVVGAGLLAATAWNLLTWRRGLPSS
                     SGHALVGGLVGAALVYGGIDAVNWGGFEGWRPVGVIGTIVALAVSPILGALAAFVVLR
                     ALRRGLRRATARVRAPVNAGQWLGSAALSFSHGANDAQKAVGVIAALLLATGHTDSLS
                     APTWATLGAAAAITLGTTLGGWRIVRTIGSRIYRIRPLDGLSSQTSSAGVILGASFLG
                     APISTTHVVSSSIVGVGVGRRRWRHVRWEIVREMGMAWVITIPATALLAGVAHTVWSW
                     VG"
     gene            9317..10750
                     /locus_tag="AC150V_06855"
     CDS             9317..10750
                     /locus_tag="AC150V_06855"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HIR3762678.1"
                     /translation="MATLLAKNAEILVTMDDDRREIVGGGIFVRDGFVEQVGPTAALP
                     SDADVVLDMSGQIVLPGFVNTHHHLDQTLTRNLPVAQELNLFPWLKAHYRVWARKTPE
                     ATRTSTLIGLAELALSGCTTVFDHSYVWQSGSRVDDQIAAAEELGSRFHVSRGSMSLG
                     ESKGGLPPDECTEDEDFILEDSERVIGAYHDMSRGSMVRIVLAPCSPFSVSGDLLKNS
                     AELARKHEGVGLHTHLCETSDEERYTLETYQLRPVEWMETLDWLGDDVWFAHAIHVNA
                     DEIKKFAATGTGAAHCPCSNMRLASGIAPVKDYRAAGVKVGIGVDGSASNDASNMLLE
                     VRQALLLARLKIGLLPPAGPETVLSTSDPRRRGEWMTAREVLEIGTRGGADVLGRDDV
                     GHLAPGMCADFFSFDLNTIDFAGGLHDPVAAVVFCAPQRAKTTVINGKVIVEEGQITT
                     VDMQPVVEEHNALAAAMADGGPPGVSV"
BASE COUNT         1769 a         3736 c         3559 g         1718 t
ORIGIN      
        1 acaacccgca caccaagccg cttccgttct gggagtggtg tatcggcgag ctcaagaccg
       61 ctcacccgga tctcgtgttt ctctccgagg ccttcacgag gccgaaggtc atgcaccggc
      121 ttgcgaaggc gggcttcacg cagtcgtaca cctacttcgc ctggcggaac acgaagtggg
      181 agctgacgga gtacttcaag gagctctcga aggaggactc gcgggagtac ttccgcccga
      241 atgcgtggcc gaacaccccc gacatcctga ccgagctcct ccagttcgga gggcggcccg
      301 cgttcgtcag caggctcgtc ctcgccgcca ccatggcagc gagctacggt atctacgggc
      361 cggcctacga gctctgcgag cacgagccgc gcgagccggg cagtgaggag tacctcgact
      421 cggagaagta cgagatccgc tcctgggacc tcgaccgagc cgacagcttg cgcgacctga
      481 tcggccgcgt gaacacgatc cgccgggaaa atccggcgct gcagcaggac tggacgctcg
      541 agttccacga ggtcgactcg gatcagctca tttgctactc gaagaccacg gacgacttcg
      601 agaacgtggt tctcgtggtg gtgaatctcg acccgcacca cacgcaggcg ggctgggtca
      661 atctctcgct cgacgagctc ggcctggacg aacagccctt ccaggtacac gacctgctcg
      721 gcggcagccg gttcctctgg caggggtcgc acaactacgt ggagctcgac ccgcaggtgc
      781 tgcccgccca catcttcctg gtgcgccgcc gcgtccggac cgagcgcgag ttcgactact
      841 acctgtgagc cacgcgacga catccggacg gcccggcacc gagtcggttc gatcgcacga
      901 tccactctgg ttcaaggacg ccgtcatcta ccagctgcac gtccgtgcct ttcacgacag
      961 ctcggggaac ggcatcggcg acttcagcgg actcaccgag aagctcgact acctccacga
     1021 cctcggcgtg accgcgttgt ggctgctccc gttctacccc tcaccgctgc gcgacgacgg
     1081 ttacgacatc gccgacttcc ggggcatcca ccccgactac ggatctctgc gggagatgca
     1141 gcgcttcctg cgggacgcgc atcggcgcgg cctgcgggta atcaccgagc tcgtcgtcaa
     1201 ccacacctct gatcagcacc cgtggttcca gcgtgcgcgc cgcgcgcccg ccgggagccg
     1261 ctggcgtgac ttctacgtct ggagcgacac gcccgaccgc taccgggacg cacgcatcat
     1321 ctttcaggac ttcgagccgt ccaattggag ctgggatccg gttgcgaacg cctacttctg
     1381 gcatcgcttc tactcgcacc agccggacct gaactacgag agccccgacg ttcgacgcct
     1441 catcctccgg acgctcaact actggttcaa cctcggggtg gacgggctcc ggctcgatgc
     1501 ggtcccctac ctctacgagc gcgaggggac gatgtgcgag aacctccccg agacgcacga
     1561 ctatctgaag gagctccggg cgcacgtcga tgagcgcttc gagaaccgga tgctcctggc
     1621 cgaggcaaac caatggccag aggatgcggc cgcatacttc ggcgacgggg acgagtgcca
     1681 catggcgttc catttcccgg tcatgccgcg gctgttcatg gccgtccaga tggaggatcg
     1741 atatccgatc atcgacatcc tccagcagac gccggcaatc cctgactcct ctcagtgggc
     1801 tctgttcctc cgcaaccacg acgagctgac gctggagatg gtgaccgacg aggagcggga
     1861 ctacatgtat cgcgtctatg cacacgaccg gcaggcgcgg atcaacctcg ggatcaggcg
     1921 gcgcttggca ccgctgctcg ggaacaacag ggcgaaagtc gagctgctct acggcttgct
     1981 gttctccctg ccggggacgc cgatcgtcta ctacggcgac gagatcggga tgggcgacaa
     2041 cgtctacctc ggagaccgcg attcggtgcg caccccgatg caatggagcg gcgaccgcaa
     2101 cgccggcttc tcccgggcga acccgcagca gctctatcta ccggtcgtga tcgaccccga
     2161 gttccacttc gagacggtca acgtcgagac gcagcttgcc aacccgcatt ccctgctctg
     2221 gtggatgcga cgcctgatcg ctttgcgcgc cagacaccac gtgttcgggc ggggcagtct
     2281 cgagttcctc acgcccgaga accacaaggt catcgctttc gtccgccggg acggcgacga
     2341 gatgatcctc gtcgtggcga acctgtcgcg gttcgctcag ttcgtcgagc tcgatctggc
     2401 ggagttcaag ggactcgtgc ctttcgagct gttcggccgc acggccttcc cgccgatcgg
     2461 ggagctgccg tacctcctca ccttgggccc gcacgccttc tactggctct cgctcgagca
     2521 accgcgggac gaggttccgg gagtcgccga cgacggtcac gccgtgacga tctcggtacc
     2581 gggacgctgg aatgaggtgt tctcgggcaa gacgaagcgc gcggccgagg actcgatctc
     2641 gcgcttcatg cagcgtcggc gctggttcgc gggcaaggcg cggtcggtgc acgacgtgtc
     2701 ggtccgcgac gtggtgtccg ttcccatccc ggagcgggat ccggcgatgc tcgtcttggt
     2761 cgacgtcgag tacgtcgagg gcgagcccga ggcctacatc cttccgctcg ctgtcgccgt
     2821 cggagagccg ggcgagcgga tcgccgtcga cgacccgcac gcagtcgtgg ctcggctcgc
     2881 acgtgggtcg gaggcggctg tgctctacga cgcgctccac gacgagaggt tctgcgccgc
     2941 cctgctcgac gcggttgcga agcggcgccg gtttcgcggc tcgaagggat cggtggtcgg
     3001 ccagcccaca ccgtcgctcc ggcggattct cgccgacgtc gaaggccaac tcgaaccgga
     3061 tctcttccgg gccgagcaat cgaacagctc cgtcgtcttc gggcagcgcc tcatcctcaa
     3121 gctctttcgg aggagcgagg agggcgtcaa tcccgacctc gagctgggca tcttcctcgg
     3181 gcaacgggca ttctcgcatg ctcccgccgt cgcgggatcg ctcgagtacg agcagtcggg
     3241 tcgcgagccg atcacgctgg cgatcgcgca cgaggtcgtc gccaacgagg gtgacgcatg
     3301 gcgctacacg cgcgacaccg tcggccactt ctacgagcgc gtcctcaccg agaacggcgg
     3361 ccttgcgacg ggcggcggcg ccagggagcg acctctcatc gagctcgtcg gcgaggagct
     3421 gccgtccgtc gcccacgcta cgagcgaggc gtacctgcgc tcggcagagc tcatcggcca
     3481 gcgcaccgcg gagctccaca acacactcgc gtccggggtg gacaccgagg cgttcgcacc
     3541 agagcccttc acgcccctct atcagcgctc ggtgtaccag tcgatgcgga atctcacccg
     3601 gcgcaacttc cgcctcctcc ggcggtcgct gccggtgctc gctccgcgcg agagggccca
     3661 ggccgagcga atcctcgcgg ccgagagtga cctcctcgag cgcttccgcg ccgttctcga
     3721 ccggacgctg tcggggctgc ggatccgcta tcacggtgac tttcacctcg gtcaggttct
     3781 ctatacgggg aaggacttcg tgatcatcga cttcgagggc gagccggcgc gctccctcgg
     3841 cgaccgcaag atcaaacgat ctcccctgcg cgatgtcgcc gggatgcttc gctcgttcga
     3901 gtacgcgggc tacgcgggac tcttcgacca ggtcgaacga ggcacggtcg aggccgagtc
     3961 ggatacgttt cgcctgctcg agggctggtg ccgccgctgg tcgtcgtggg cctcggtgtc
     4021 ctttctgcgg tcgtacctcg agacggccga cgaccactac ctccccggaa cgcgcgagga
     4081 gctgcacagc gtcatcgacc tgttcttgct ggagaaggcc gtctacgagc tcggctacga
     4141 gctgaacagc cgacccgact gggtgaggat cccactcgcc ggcatcctgc gactcctcgg
     4201 agagcggccg tgactcggcg cgcgaagggg aacgactccg tgcctccggc gaaggagcgc
     4261 cgccaggaca tcgtcgaccc cggagaggcc ttcctcggcg acgacgacct ccatctcttc
     4321 aacgaaggaa cgcacacgag gctgtacgag aagctcggag cgcacgtcgc cgcgctggac
     4381 ggaacggccg gcgtgcagtt cgcggtctgg gcgcccaacg ccgagcgggt gtccgtcgtg
     4441 ggcgacttca acggttggga ggctggaagt catccgctcc ggccacgcgc ctcgtccggt
     4501 atctggcagg gcttcgttcc cgagctcccg tccgggtcgg tctacaagta cgcgatcgtc
     4561 tcgaagacag gcgatcaccg agtggagaaa gccgacccgt tcgcctttgc ggcggagatc
     4621 cccccaaaga ccgcgtcggt cgtctgggag ctcgattacg agtggggcga cggcggctgg
     4681 atgaagagac ggatggacgc caacgcgctc gacgcgccga tctcgatcta cgagatgcat
     4741 ctcggctcct ggcgccgcgt gcccgctgac ggcgaccggt cactgagcta cctcgagctt
     4801 gccgaagccc tgccggacta cgtccgcgag cgggggttca cccacgtcga gctgatcccg
     4861 ctcatggagc atccgttcta cgggtcctgg gggtaccaga cgaccgggtt cttcgcgccg
     4921 acgagccgct acgggacgcc gcaggacttc atggcgctgg tcgacgcact gcaccaggcg
     4981 gggatcggag tcatcctcga ctgggttccg tcccacttcc ccgacgacga gcacgggctc
     5041 gcctacttcg acggctcgca tctgtacgag cacggcgacc cgcgcgaggg acggcatccc
     5101 gactggggca gcttgatctt caactacgga cgccacgagg tcaggagctt cctcctctcc
     5161 agcgccctgt actggctcga ccggtaccac gccgacggcc tgcgtgtcga cgccgtcgcc
     5221 tcgatgctgt accgggacta ctcgcggaag gagggagagt ggataccgaa cgagcacggc
     5281 ggccgggaga acctcgaggc gatcgggttt ctccggcagc tgaacgagga ggtgtacggg
     5341 cagtatcccg acgtccagac gatcgccgag gagtcgaccg cgtggcccat ggtctcccgt
     5401 ccgacctacc tcggcgggct cggattcggg ctcaagtggg acatgggctg gatgcacgac
     5461 acgctccact acatggctcg tgaccctgtc ttccggccgc accaccacaa cgagctcacg
     5521 ttccgagcga tctacgcctc gaacgagaac ttcctacttc cgctctccca cgacgaagtc
     5581 gtgcacggga agggctcgct gctcggcagg atgcccggcg acgaatggca gaagctcgcc
     5641 aacctgcgac tgctctacgg ctaccagtgg gcccaaccgg gcaagaagct gctcttcatg
     5701 ggcggcgagt tcgcgcaagg ttcggaatgg aaccacgacg cgaatctcga gtgggagctc
     5761 gtcgagcgcc cgacccaccg cggagtccaa cgctggatcg acgatctgaa ccgcgtccac
     5821 cgcgaagagg ccgcacttca cgagctcgac tgcgaccccg ccggattcga ttgggtcgac
     5881 gccaacgact ccgcggccag cgtcgtggcc tttctccgcc gaagccgcac gggcgaggaa
     5941 gtgctcgtca tctgcaactt cacccccgtc ccgcgccaca actaccgcgt cggcgtcccg
     6001 accggcggcg tgtgggagga actgctcaat tcggacgccc atgactacgg cggcagcggg
     6061 caggggaacc tcggcggcat cgaagcgacg cccgtcccct accacgggcg acggaagtcg
     6121 ctcgtcctca cgctccctcc gcttgcggcg ctcttcgtca agcctcagcg gtcgggcgac
     6181 gcttgaggcc acgctggctc gacctccccc gctccagcgg gatcctcctt cacccgacgt
     6241 cgcttccggg cggtcggctc ggcgccggcg cgcgctcgtt cgtggactgg ctggctgcgg
     6301 cggggcaatc gtggtggcag gtgcttccgc tcgggccgcc cgacgaccac tgctcaccgt
     6361 acaagccgac gtccgcgttc gcgggctcgc cctccctcct cgccgagccg gacgcacgcg
     6421 tagacgccgc ggaggtagac cgcttcgtgg agaagcactc gtactgggct tccgactggg
     6481 cggaatacgc gggcgacgga gcgctcgagg accaggttcg cttcgagcgc gagtggagcg
     6541 agctccgcgc ctacgcggca gcccgaggag tccgactgat cggagacatc ccaatctacg
     6601 tggctgaggg aagcgccgac cacgtttccc acccggagct cttccaggac gacgtcgtcg
     6661 ccggagtccc tcccgacgac ttctccgcca cgggtcagct ctgggggaat ccgctctacg
     6721 actggccggc gctccggcgg acaggctacg agtggtggat cgaacgcttc caccgcgtgc
     6781 tcaagcttgt cgatctcgcg cgtgtcgacc acttccgcgg cttcgtcgcc tactgggcgg
     6841 tcgccgcggg gagccctacc gcgatgcacg ggcggtggcg ccgcgggccg ggcatggcgc
     6901 tcttccggga ggccgaagcg gcgctcggcc cgctgccgct cattgccgag gatctcggtg
     6961 tgatcacccc tcccgtcgtg cggttgcggg acgaactcgg tgtgccgggg atggtgattc
     7021 tccagtacgc gttcggcgac gacggtcgca gtccgcacgt cctcgcgaat caccccgagt
     7081 gcgcggtcgt gttcacgggt acgcacgatc atgcgccgat cgcggcgtgg tggacggagg
     7141 aggcacactc cggcgagcgt gaccgggccg acagagccgc ggcggcgctc ggcatcagcg
     7201 atcccgagtc acactggcga cttctccggc tggccttctc ttcacgtgct cggctcgcga
     7261 tcgcacccat gcaggacgtg ctcggcctgg gcagagaggc acggatgaac acgccggcga
     7321 ccgacgccgg gaactggggc tggcagcttc cggcaggcca gctcacgagc gagctcgctg
     7381 atcgtctcag gacgtcgacg gaagcggccg gccgcaaggc ctagctgcgg cgtcgggaac
     7441 gggcatcgcc ccacgcaggc gcagcttcgg tgcaccgacg agagagcctc aagactcctt
     7501 gatgaccgcg taccagaagc gctcgccgac gtcgctgacg acctcgctga tgcgcgagaa
     7561 gcggcggtag agctcgcgac gcgacatgac gatccggaaa tcgtcgacct cgaggagttc
     7621 cgccatcgcc cgccggtaga cgcgctcgag ccgtcggttg gccttgatcg ccgcgtcggc
     7681 cgctttcgtc gcccggtcgt cgcgggcctg cagtcccgcg accgcctcgc cgagactgcc
     7741 gacggcatcg cgtagcagcg ctgccatctc ggcgatcggc gcgtcgggcg agcagtccat
     7801 gacctccgac tcgcgcaccg cgtccttgga gagattgagg atccagtcga taccgcgact
     7861 gagcgtgtag atgtcctcgg cgtcgtaggg cgtcgtgaac gcctccacga gtcctcgctg
     7921 gagcgcgcgc ttgtgctcgt ccgcttcgtg ctcggtcgcg cgcaccacct cggcggcaga
     7981 cgcgtccccg ttcgcccact cgacgagcgc gtccatgccc cgcttcgtca cctcgatctg
     8041 ggccttgagc atgccgagga catcgggcga gtcgggcagg aaccagcggc tcgcggtctt
     8101 catccgaccc aactccagac cgtatgggcg acgcccgcca gcagtgccgt cgccggaatc
     8161 gtgatgaccc aggccatgcc catctccctc acgatctccc agcgcacgtg gcgccagcgc
     8221 cgacgcccca cgccgacgcc gacgatggac gaggagacga cgtgcgtggt cgagatcgga
     8281 gcgccgagga acgaggctcc caggatgacg cccgccgagc tcgtctggct cgagagcccg
     8341 tcgagcggcc ggatgcgata gatgcgactt ccaatcgtcc tcacgattct ccagcccccg
     8401 agggtcgtcc cgagggtgat cgccgcggcg gcacccaacg tagcccacgt cggcgcggac
     8461 agggagtccg tgtggccggt cgcgagcagt agtgcggcta tcacgccgac ggctttctgt
     8521 gcgtcgttgg cgccgtgact gaacgacagc gctgccgatc cgagccactg gccggcattg
     8581 accggtgcgc ggacgcgggc tgtggctctg cgcaatccgc gccgcagcgc tcgcaagacg
     8641 acgaaggcgg ccagggcgcc gaggatggga gacacggcga gcgccacgat tgttccgatg
     8701 acgcccaccg ggcgccagcc ctcgaagccg ccccagttca cggcgtcgat gccgccgtag
     8761 acgagcgccg cgcccacgag gccccccacg agtgcgtgcc ccgagctcga cgggaggccg
     8821 cggcgccacg tgaggaggtt ccaggccgtg gcggccagaa gcccggctcc gacgacggtc
     8881 accgtctcgt tcgccggcac gtcgacgatc gtggcgatcg tgttcgccac cgccgacccc
     8941 acgagcagcg gcccgaggaa gttgaagacc gccgcgagga tgacggcccg cgagggccgt
     9001 gcggcgcgcg tggcgacgag ggcggcgatc gaattggaag cgccgtggaa cccgtttgtc
     9061 agcgcgaacg cgaacgcgag cgcgacggcg acgacgagac tcgtgtccac tcgttgaacc
     9121 tactggctcc tggcgctccg agaccgatgg gcctcgagag gcctctgatc agctcatgac
     9181 gcacggcgcg ggaactcgca gcggagacaa gcaggggcga cactacagac gcccctgctt
     9241 gggcgtttcg cggcgcctct ccggagatct gcctcgaacc ccatgcagga actcgacgtg
     9301 aaggagtgac gaaacgatgg caacgctgct ggcaaagaac gctgaaatcc tcgtcacgat
     9361 ggacgacgat cgccgggaga tcgtcggcgg cgggatcttc gtacgcgacg gcttcgttga
     9421 gcaggtcggc ccgacggccg cgctgccctc cgacgcggat gtcgtcctcg acatgtcggg
     9481 acagatcgtg ctgccgggct tcgtcaacac gcaccaccac ctcgaccaga ccctcacgcg
     9541 caacctcccg gtcgcgcagg agctcaacct ctttccctgg ctgaaggccc actaccgcgt
     9601 gtgggcccgc aagacgcccg aggctactcg gacgagcact ctgatcgggc tggccgagct
     9661 tgccctctcc ggctgtacga ccgtgttcga ccactcgtac gtctggcaga gcggctcccg
     9721 ggtcgacgac cagatcgccg ccgcggagga gctcggcagc cgcttccacg tctcccgcgg
     9781 aagcatgtcc ctgggcgagt cgaagggcgg cttgccgccg gacgagtgca ccgaggacga
     9841 ggacttcatc ctcgaggact ccgagcgcgt gatcggcgcg tatcacgaca tgtcacgcgg
     9901 ctcgatggtg cggatcgtcc ttgcgccgtg ctcgccgttc tcggtcagcg gcgacctgct
     9961 caagaactcg gccgagctgg cccgcaagca cgagggagtc ggccttcaca cccacctctg
    10021 cgagacgagc gacgaggaac gctacacgct cgagacgtac cagctgcgcc cggtggagtg
    10081 gatggagacg ctcgactggc tcggcgacga cgtgtggttc gcgcacgcga tccacgtgaa
    10141 cgcggacgag atcaagaagt tcgccgccac gggtacgggc gcggcccact gcccctgctc
    10201 gaacatgcga ctcgcatcgg gcatcgctcc ggtcaaggac tatcgcgcag ccggcgtgaa
    10261 ggtcggtatc ggcgtggacg ggtcggcgtc gaacgacgcc tcgaacatgc tcctcgaagt
    10321 gcgtcaggcg ctgctgctgg cgcgcctcaa gatcgggctg ctgccgccgg cggggcccga
    10381 gaccgtgctc tccacgtcgg acccgcggcg ccggggcgag tggatgactg cgcgcgaagt
    10441 cctcgagatc ggcacccgcg gcggcgccga cgtgctcggc cgggacgacg tcggtcacct
    10501 cgcgccgggg atgtgcgccg acttcttctc cttcgacctg aacacgatcg acttcgcggg
    10561 cggcctccac gatcccgtcg cggcagtcgt cttctgcgcg cctcagaggg cgaagacgac
    10621 ggtgatcaac ggcaaggtga tcgtcgagga gggtcagatc acgaccgtcg acatgcagcc
    10681 cgtggtcgag gagcacaacg cgctcgccgc cgccatggcg gacggcgggc ctccaggagt
    10741 gtccgtctga gccgccgagg cggccctagg ggccaggccc gc
//
LOCUS       DBNGNK010000232         3334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_185940,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000232 DBNGNK010000000
VERSION     DBNGNK010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_185940"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..275
                     /locus_tag="AC150V_06860"
     CDS             66..275
                     /locus_tag="AC150V_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762679.1"
                     /translation="MTILDEPSRRRSGRRRRRVWPRVLALVLGLLVVFALGVALGRAL
                     EDAPEPGVTITSVRTLEPIPQQTAP"
     gene            complement(282..734)
                     /locus_tag="AC150V_06865"
     CDS             complement(282..734)
                     /locus_tag="AC150V_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762680.1"
                     /translation="MESSRSRPSVSSAFDRSAVLLGLGDRLTLIVGLVLTISAFTGWY
                     TGSGEGVTVSVMGWHTGTLGKLVFFIGLAVILVVALRELGVELPAAVPESLITIGLGS
                     LATIFVLIRVISIPDDFFFAGRGVGIWISLVAAIGVIVAGLLQAAEEL"
     gene            790..1023
                     /locus_tag="AC150V_06870"
     CDS             790..1023
                     /locus_tag="AC150V_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762681.1"
                     /translation="MAERVRDVAPDDAPPVDPHAVSARYRRAKAKRRARVKRRRATKW
                     AGFRFWLVLLALVAASVVIVVVILREVEQLFGL"
     gene            1044..2582
                     /locus_tag="AC150V_06875"
     CDS             1044..2582
                     /locus_tag="AC150V_06875"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR3762682.1"
                     /translation="MVDLGGPYGRQALGVVVFIAGAAALGTEIAASRMLAPYFGSSTI
                     VWANLIGVVLAALSLGYWIGGRLADRRPHSGVLGTIILASAVLVALTPFVAKPFLELT
                     VEGLNEASAGAVVGSFIAVLLLFAPPVVLLGMVSPFAIRLAVSGVATAGSVAGRLYAI
                     STVGSLIGTFLSAILLIPLIGTQRTLLLLAALLAIAGSVLAGVRYLVLALAVAALVAV
                     PPGVVKAEPGLIHEETSLHQYIQVVEREDGRRLLFLNEGVAVHSVWRADEVLTGGVWD
                     AFLAVPPLLGRPLEKVAILGNAGGTVARAMGVYFPAAKIDGVELDPAVSSVGRRWFGL
                     EDNPNLTVHDADARPFLRSTDERYDLIVIDAYHQPYVPFYLATRDFFRLVSERLAPGG
                     VVALNVASVPEDSRLVDAIGGTLAAEVPQVLEWPALSFNSLVLGLTEPMSSETRAARL
                     STGHPDLAPLRNLLARETRDIERAERPWTDDRAPVEWVTDQMIVSFAARGGRLDEDYL
                     PTRP"
     gene            2579..>3334
                     /locus_tag="AC150V_06880"
     CDS             2579..>3334
                     /locus_tag="AC150V_06880"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR3762683.1"
                     /translation="MIDLWRRDGRPLRIGHRGAATLAPENTLAAFRAATAAGVDMIEF
                     DVLDLPRGPLIVAHSDHLDEVSHGAAQGSVRALSMEQLRRVAPDVPTLDEALDFFVHE
                     AAGVGVHVDLKLRSRVDEVAAAIERHGLAARTVVSSFHSAPLREVARRSRTIRIGFTY
                     PEDRFSVSRRPRLWPLVRVGLSTLRATVPRRLPYLLRRAGASALMLQHALVTPAAVVR
                     AHELGVPVLAWTVDEPDDLERVESAGVDGVITND"
BASE COUNT          467 a         1160 c         1143 g          564 t
ORIGIN      
        1 tcggtggcgg tcgcaggtga cgcggccttt cacctctatg cccgcgccgc ggtcgactac
       61 acgccgtgac gatcctggac gaaccctccc gccgtcgctc cggccgccgg aggcgtcggg
      121 tctggccgcg agtcctcgcg ctcgttctgg gtctgcttgt cgtcttcgcg ctcggcgtcg
      181 cgttggggag ggcattggag gacgcgcccg agcctggcgt cacgatcacg tcggtgcgga
      241 cgctcgagcc gattccgcag cagaccgccc cctgagccgg gctagagctc ttcggccgcc
      301 tggaggagtc cggcgacgat cacgccgatc gcggcgacga ggctgatcca gatccccacg
      361 cccctgccgg cgaagaagaa gtcgtccggt atcgagatca cccggatcag cacgaagatc
      421 gttgccagcg agccgagccc gatcgtgatc aagctctccg ggaccgcggc gggcagttcg
      481 acgccgagct cccgcagcgc cacgaccagg atcacggcca gcccgataaa gaagacgagt
      541 ttgccgagcg tgccggtgtg ccatcccata accgacacgg tgacgccttc ccccgagccc
      601 gtataccagc ccgtgaaggc tgagatggtg agcaccagcc cgacgatcag cgtgaggcga
      661 tcgccgaggc cgagcagcac cgcgctacgg tcgaaggcag aggaaacgga cggtcgcgac
      721 cgcgaagatt ccatggcccg agaagtatct tcgatgcagc ggccggagtc acgggcttac
      781 actttcccta tggctgagcg cgtgcgagac gtcgcgcccg acgacgcgcc gcctgtcgac
      841 ccgcacgcgg tgtcagctcg ataccggcgg gcgaaggcga agcgtcgggc gcgggtcaag
      901 cgacgacgtg cgacgaaatg ggccggattc aggttctggc tcgtcttgct cgcgctggtt
      961 gcggcgagcg tcgtcatcgt cgtggtgatc ctgcgtgagg tcgagcagct gttcgggttg
     1021 tagagaccgg gcgtgacgta gcaatggtgg atctcggagg cccgtacgga cgtcaggccc
     1081 tcggagtggt cgtgttcatc gcgggcgccg ccgcgctcgg gacggagata gccgcctcac
     1141 gaatgctcgc tccgtacttc ggctcgtcga cgatcgtctg ggcgaacctg atcggtgtcg
     1201 tgctcgcggc gctatcgctc ggatactgga tcggtggacg actcgccgat cgaagacctc
     1261 actcgggcgt gctggggacg atcatcctcg cctcggccgt gctcgtggcc ctgacgccgt
     1321 tcgttgccaa gccctttctc gagctgacgg tcgagggtct caacgaagcg tccgccggtg
     1381 cggtcgtcgg ttcgttcatc gctgtgctgc tcctcttcgc cccgccggtc gtcctgctcg
     1441 ggatggtctc gccctttgcc atcaggctcg ccgtcagcgg cgtcgcgacg gcagggtccg
     1501 tggcgggccg tctctacgcg atctcgacgg tgggctcact gatcggaacc ttcctctcgg
     1561 cgatcctgct catcccgttg atcgggacac agcggacgct gctcctgctg gcggcgctgc
     1621 ttgcgatcgc gggctcggtg cttgccggcg ttcgctacct cgtcctggcg ctcgcggtcg
     1681 cggcgctcgt ggccgtcccg cccggggtgg tcaaggccga gccgggactg atccacgagg
     1741 agacatcgct ccaccagtac atccaggtgg tcgagcgcga ggacggacgc cgtctgctct
     1801 tcctcaacga gggcgtcgcc gtgcactccg tctggcgtgc ggacgaagtg ctcaccggcg
     1861 gcgtctggga cgcctttctc gccgtgccgc cgctgctcgg acgtccgctc gagaaggtgg
     1921 cgattcttgg caacgcgggt ggaacggtgg cccgcgcgat gggcgtctac ttcccggctg
     1981 cgaagatcga cggcgtcgag ctcgatccgg ctgtcagcag cgtcggccgg cgctggttcg
     2041 gactggagga caacccgaac ctgaccgttc acgacgcgga tgcgcgaccg ttcctgcgct
     2101 cgacggacga gcgctacgac ctcatcgtga tcgacgccta tcaccagccg tacgttccct
     2161 tctacctggc gacgcgggac ttcttccgcc tcgtctcgga gcgcctggcc cccggggggg
     2221 tcgtagcact caacgtggcc tccgttcccg aagacagccg gctggtcgac gcgatcggcg
     2281 gcaccctcgc cgccgaggtg ccgcaggtgc tcgagtggcc ggcgctgagc ttcaactccc
     2341 tcgtgctcgg cctgactgag ccgatgagct ccgagacgcg ggccgcccgt ctgtccaccg
     2401 gccaccccga cctcgcaccg ctgcggaacc tgctggcgcg agaaacgcgt gacatcgagc
     2461 gcgcggagcg cccctggacg gacgatcgcg ccccggtcga gtgggtcaca gaccagatga
     2521 tcgtctcgtt cgcggcccgg ggtggtcggc tcgacgagga ctacctcccg actcggccgt
     2581 gatcgatctc tggcgtagag acggccgtcc ccttcggatc ggccaccgcg gcgcggcgac
     2641 gctggcgccg gaaaacaccc tggcggcctt ccgcgccgcg acagcggccg gcgtcgacat
     2701 gatcgagttc gacgtcctcg acctaccccg gggcccgctg atcgttgctc actccgacca
     2761 cctcgacgag gtcagccacg gcgccgcgca gggaagcgtc cgggcgctga gcatggagca
     2821 gctccgccgg gtcgcgccgg atgttcccac cctcgacgag gcgctcgact tcttcgtgca
     2881 cgaggcggcc ggtgtgggag tgcacgtcga cctgaagctg cgttctcggg tcgacgaggt
     2941 cgccgcggcg atcgagcgtc acgggttggc ggcacgaacg gtcgtcagct cgttccactc
     3001 cgcgccgctg cgcgaggtcg ctcgtcgctc gaggacgatc aggatcggct tcacgtaccc
     3061 ggaggatcgc ttcagcgtct cgcgcaggcc gcgtctctgg ccgctcgtca gggtcggcct
     3121 gtcgaccctg cgcgcgaccg tgccgcgtcg ccttccgtac ctgctgcgcc gcgccggcgc
     3181 cagcgccctc atgctccagc acgcgctcgt cacgcccgcc gcagtggtcc gggcgcacga
     3241 gctcggcgtc cccgtactcg cctggacggt cgacgagccc gacgacctcg agcgggtcga
     3301 gtccgcaggg gtggacgggg ttatcacgaa cgat
//
LOCUS       DBNGNK010000233         5505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1188204,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000233 DBNGNK010000000
VERSION     DBNGNK010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1188204"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="AC150V_06885"
     CDS             <1..728
                     /locus_tag="AC150V_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-phosphoglycerate kinase"
                     /protein_id="HIR3762684.1"
                     /translation="ELAEDVLGSESASVTMRRIRRLQALHDLDVPVILLVGGATGTGK
                     STLATEAAHRLGITRVASTDFIRQTMRAFFSKEFMPSVHYSSFEAALALTKAEEEEAG
                     NAVVLGFLDQVRNVIVGVEAAIDRALTERWSMVLEGVHLVPGMTITSRDDALVVHCIV
                     TISDEETHRSNFWVRDGATEGLRPLDKYMDGLPEIRLVQELLIERAKRFDTPLIENRS
                     LDQAIGEVMDLVLTEAERLVRTT"
     gene            734..1681
                     /locus_tag="AC150V_06890"
     CDS             734..1681
                     /locus_tag="AC150V_06890"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HIR3762685.1"
                     /translation="MCTSYAKATEWAALAGARWLGRGDGAAAEEAAAGGMKSALESMP
                     ITGRVIIGARSGEFLAQDESVGSGGDAVDLALDPLEGASVVARGGTGAMSMLAAASPG
                     SLRRLPEMYMRTMAVGPLAKDAVDLHRPVGENLQAIASSFGRNPADITTIVLDRPRHH
                     DLIEEIQEAGSRIKLIQDGTVTASISAAIRGTNDHLAVGIGGTRQALLSAAALRCLGG
                     RVQAQLWPTTRTQIEEAAQAGIADVSQVFDEEALVSGDVIVVATGVSNGDLLRGVRFL
                     ADSARTHSIILCMRCNWVRFTDGIHFFARDRREEVRLLS"
     gene            1748..2056
                     /locus_tag="AC150V_06895"
     CDS             1748..2056
                     /locus_tag="AC150V_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR3762686.1"
                     /translation="MVTKEQVVEALHMVEDPELGMDIVELGLFYDAEIEGPKVKVRYS
                     LTSMACPVGPMIQEDIHNTVAAIEGVDEVETELTFDPPWTPDMMSDDAKFILGFDVPL
                     "
     gene            complement(2067..2861)
                     /locus_tag="AC150V_06900"
     CDS             complement(2067..2861)
                     /locus_tag="AC150V_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3762687.1"
                     /translation="MSLPVASSALRRDRKSTVVWWLGVAALVAITIAFYPTVAGNDEL
                     DRIWEDLPESLQALFGATDLTSPVGYLESQLFAATLPIVLLVWGIGRANDAVAGEEQQ
                     GTLDLSLAAPLTRRSFALQKLLSLGGLLAVLLTGAVVPVLALKAAVDLDVGAANLVTR
                     TVLLGLLVFFFATVAYAVSAGSGRKGVAVGVTAGVAVAAYLLDSLGQVVGWLEPLRVL
                     SPFSWSTEGDPLRNGFDWSGTAALVVASLALLGAGTWLFERRDLSA"
     gene            complement(2858..3751)
                     /locus_tag="AC150V_06905"
     CDS             complement(2858..3751)
                     /locus_tag="AC150V_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762688.1"
                     /translation="MESPAITAEGLTKFYGGNRGIEDITLDVGRGEVFGLLGPNGAGK
                     TTFIRSLLDLIHPTRGRALVLGLDSRRDGVELRRRIGYVPGELALYDTLTGREHIRYF
                     AGLRSGAGENHAFAVADRLDLDVERPAGELSKGNKQKLGLALAFMHEPEVLLLDEPTS
                     GLDPLLQEEFRSLLAESAQNGATVVLSSHVLAEVEHAADRVAILREGFLVAVAGMDEI
                     KRRAVRRIHVRFGEPVEKSSFDDVPGVREVLGDGPEITLVTEGAMDAVVKALSRYEVV
                     SLVSNEVDLEDVFMSYFRGPT"
     gene            complement(3756..4742)
                     /locus_tag="AC150V_06910"
     CDS             complement(3756..4742)
                     /locus_tag="AC150V_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3762689.1"
                     /translation="MKAIRIHEDGGPEVLRYEDVPDPEPASGEILIRLHAASLNHLDI
                     WVRKGLPSVPKPRILGGDGAGVVEALGDGVSDFETGARVVINPGLEHGDTIGVVGEHS
                     DGTHCELIAIAAEQVFALDDALSFEEAAAFPLVYETAYRMLVTKAALQPDEWVLIWGV
                     GGGVGIAAFELARALGARTVVTSGSDWKVERAREWGADLALNHDTDDVVAAVKEATGK
                     GVHVVVESVGEATWARSLAAVRPAGRVTVCGATSGPNPPAQLHRFWWKQLTVLGSTMG
                     TREDFLGAYELVRSGRARVHVDRVFPLAEAQAAHERLEAGEQLGKIVLAIPG"
     gene            4759..>5505
                     /gene="queG"
                     /locus_tag="AC150V_06915"
     CDS             4759..>5505
                     /gene="queG"
                     /locus_tag="AC150V_06915"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR3762690.1"
                     /translation="MRAHELARLAEELGLDAIGATPARPYEETERHIRERRAKGLFAE
                     MRFTMAQPEVSCHPEKLFPGAATVVSAALCYYVPGGEPGLGEGRLPRYTWRDHYALLR
                     ERLSALGERLGGEYRVLVDANQHVDREGAARGGVGFYGKNTMLITPSHGSWVVLGTLV
                     TDVAIEPTSPVELDCGSCRLCIDACPTGALDDPGVLDSTKCIAYWAQAPRPIPSEYRF
                     ELAQNAYGCDVCQDVCPWNHPIEKRRADSAL"
BASE COUNT          897 a         1854 c         1910 g          844 t
ORIGIN      
        1 gcgagctggc cgaggacgtg ctcggcagcg agagcgcctc ggtgacgatg cgccgcatcc
       61 gtcgtctgca ggcgctgcac gacctcgacg ttccggtcat cctgctcgtc ggcggagcga
      121 cgggcaccgg caagtcgacg ctcgccacgg aggcggctca caggctcggc atcactcggg
      181 tcgcctcgac cgacttcatc cggcagacga tgcgcgcctt cttctcgaag gagttcatgc
      241 cgtcggtgca ctactcgagc ttcgaggcgg ccctggcact gacgaaggcc gaggaggagg
      301 aagccggcaa cgccgtcgtc ctcggctttc ttgaccaggt ccgcaacgtg atcgttggag
      361 tggaggcggc gatcgaccgc gccctgacgg agcggtggtc gatggtgctc gagggtgtcc
      421 acctcgtgcc cggcatgacc atcacgtcgc gcgacgacgc gctcgtggtt cactgcatcg
      481 tgacgatctc cgacgaagaa acccatcgca gcaacttctg ggttcgggac ggcgcaaccg
      541 aggggctccg cccgctcgac aagtacatgg acggactccc cgagatccgt ctggtgcaag
      601 agctgttgat cgagcgcgcg aagcgcttcg acacgccgct gatagagaac cggtcgctcg
      661 accaggcgat cggtgaggtg atggacctcg tcctcaccga ggcggaacgg ctggtgcgca
      721 caacgtgagc tgtttgtgca cgagctacgc gaaggcgacc gagtgggcgg cactggccgg
      781 cgcccgctgg cttggcagag gtgacggcgc ggccgccgaa gaggcggcgg cgggtggtat
      841 gaagtcggct ctcgagagca tgccgatcac ggggcgggta atcatcggcg cccgctccgg
      901 cgagttcctg gctcaggacg agtccgtcgg ctccggcggg gacgccgtcg acctcgcgct
      961 cgatccgctc gaaggggcgt cggtcgtcgc tcgcggcggc accggcgcga tgtcgatgct
     1021 tgctgcggcc tctccgggct cgttgcgccg gttgccggag atgtacatgc ggacgatggc
     1081 tgtggggccg ctcgcgaagg acgcggtcga ccttcaccgg ccggtgggcg agaacctgca
     1141 ggcgatcgcg agcagtttcg gcaggaatcc ggccgacatc acgacgatcg tgctcgatcg
     1201 gccgcggcat cacgacctga tcgaggagat ccaggaagcc ggatcgcgca tcaagttgat
     1261 tcaggacggc accgtcactg cctcgatctc ggccgcgatc aggggaacga acgaccatct
     1321 cgccgtcggg atcggcggca cacgacaggc gttgctgtcg gcggctgcgc tgcggtgtct
     1381 cggcggccgt gtgcaggcac agctctggcc gacgacgcgt acgcagatcg aggaggccgc
     1441 gcaggccggg atcgcggacg tgtcgcaggt cttcgacgaa gaggcgctcg tgtcagggga
     1501 tgtgatcgtc gtggcgacgg gcgtctcgaa cggtgatctg ctgcgcggcg tgcgtttcct
     1561 ggcggacagc gctcgcacgc actcgatcat cctctgcatg cgctgcaact gggttcgatt
     1621 caccgacggc attcacttct tcgcccgcga tcggcgcgag gaagtcaggc tgctgagctg
     1681 aggccgcgcc accgttggcg gcgccgatcg gtgcggcgcg tcgatcgggc gaggctacac
     1741 tccgcgcatg gtcactaagg agcaggtggt cgaggcgctc cacatggtcg aggatcccga
     1801 gctgggaatg gacatcgtcg agctcggcct cttctacgac gcggagatcg aagggccgaa
     1861 ggtcaaggtt cgctactcgc tgacctcgat ggcgtgcccg gtcggaccga tgatccagga
     1921 ggacatccac aacacggtcg cagcgatcga gggcgtcgac gaggtcgaga ccgagctgac
     1981 cttcgacccg ccgtggacgc ccgacatgat gtccgacgac gccaagttca tcctgggctt
     2041 cgacgtcccg ctgtagagcg gcaccgtcag gccgatagat cgcggcgctc gaacagccac
     2101 gtcccggcgc cgagcagggc gagcgaggcg acgaccaggg cggcagttcc gctccagtcg
     2161 aagccgttcc gcagcgggtc accctccgtc gaccaggaga agggtgagag cacccgcaac
     2221 ggctccagcc agccgacgac ctgccccagc gagtcgagca ggtaggcggc gacggccacg
     2281 cctgcggtga cgccgaccgc gactcccttg cgaccgctgc ccgcggacac cgcgtaggcg
     2341 accgtcgcga agaagaacac gaggagcccg aggaggacgg ttcgcgtgac gagatttgcc
     2401 gccccgacgt cgaggtcgac cgccgccttc agcgccagta cgggcaccac ggccccggtg
     2461 agcagaaccg cgagcaggcc gccgagcgag agcagcttct gcagtgcgaa gctgcggcgg
     2521 gtgagggggg cggcgagcga gaggtcgagc gttccctgtt gctcctcgcc ggcgaccgca
     2581 tcattcgcac ggccgatgcc ccagacgaga aggacgatcg ggagcgtcgc ggcgaagagc
     2641 tggctctcca gataaccgac cggcgacgtg agatccgttg cgccgaagag cgcctgcagc
     2701 gactcgggca ggtcttccca gatgcggtcg agctcgtcgt tgccggcgac ggtcgggtag
     2761 aaggcgatcg tgatcgccac cagagctgcc actccgagcc accacacgac ggtcgacttc
     2821 cggtctcgac gcagcgccga ggaggcgacg gggaggctca cgtcgggcct ctgaagtagc
     2881 tcatgaagac gtcctcgagg tcgacctcgt tgctgacgag gcttacgacc tcgtagcggg
     2941 agagagcctt cacgaccgca tccatggcgc cctcggtcac gagagtgatc tccggcccgt
     3001 cgccgagcac ctcacgaacg ccggggacgt cgtcgaacga gctcttctcc accggctcgc
     3061 cgaagcggac gtggatccgc cggacggcgc gccgcttgat ctcgtccatg ccggcgacgg
     3121 cgacgaggaa cccctcgcgc aggatcgcca cccggtcggc cgcgtgctcg acctccgcga
     3181 gaacgtgcga gctgagaaca acggtcgcgc cgttctgcgc ggactcggcc agcaggctgc
     3241 ggaattcttc ttgcagcagt gggtcgaggc cgctggtcgg ctcgtcgagc agcaggacct
     3301 cgggctcgtg catgaacgcg agcgccagtc cgagtttttg cttgtttcct ttggacagct
     3361 cgccggcggg ccgctcgacg tcgaggtcga ggcggtcagc gacagcgaac gcatgattct
     3421 cgccggcacc gcttctcagg ccggcgaagt agcgaatgtg ctcgcgaccc gtcagcgtgt
     3481 cgtagagggc aagctcgccc ggcacgtagc cgattcgccg acgcagctcg actccgtctc
     3541 ggcgcgagtc gaggccgagg acgagcgctc tgccgcgggt cggatggatc aagtcgagca
     3601 gcgagcgtat gaacgtggtc ttcccggccc cgttggggcc cagcagaccg aagacctcac
     3661 cccgtccaac gtcgagcgtg atgtcctcga tgccccggtt gcccccgtag aacttcgtca
     3721 ggccctcggc cgtgatcgcc ggggattcca acggcctagc cggggatcgc gagcactatc
     3781 ttgccgagct gctctcctgc ctcgaggcgc tcgtgcgccg cttgcgcctc ggcgagcggg
     3841 aagacgcggt cgacatgcac gcgggcccga ccgctccgaa cgagctcgta ggcgccgagg
     3901 aagtcctcac gagtgcccat cgtcgagccg agaaccgtca gttgcttcca ccagaagcgg
     3961 tggagctgtg caggggggtt cggcccgctc gtcgcgccgc agaccgtcac gcggccggcg
     4021 ggtcgcactg ccgccaaaga cctggcccac gtggcctcgc cgacggactc gacaacgacg
     4081 tgaacgccct tgcccgtcgc ctccttgacg gcggcgacca cgtcgtccgt atcgtggttg
     4141 agagcgagat ccgctcccca ttcgcgggcc cgctcgacct tccagtcact gccggaggtc
     4201 accaccgtcc gggcgccgag ggcgcgtgcg agctcgaagg cggcgatgcc gacgccgccg
     4261 ccgacgcccc agatgagcac ccattcgtcg ggctgcaggg cggccttcgt gacgagcatg
     4321 cggtaggcgg tctcgtagac gagcggaaag gcggccgcct cttcgaacga gagcgcgtcg
     4381 tcgagggcaa acacctgctc cgcagcgatc gcgatcaact cgcagtgcgt tccgtcgctg
     4441 tgctcgccga cgacgccgat cgtgtcgccg tgctcgagcc ccgggttgat gacgacccgc
     4501 gcgcccgttt cgaagtccga gacaccgtcg ccgagcgcct ccacgacgcc ggcgccgtcg
     4561 ccgcccagga tcctgggttt cggtaccgac ggcagcccct tccggaccca gatgtcgaga
     4621 tggttcagcg aggccgcgtg cagccggatg aggatctcgc cggacgccgg ctccggatcg
     4681 gggacgtcct cgtagcggag gacctcgggc ccaccgtctt cgtggatgcg aatcgccttc
     4741 acgcgtcaca atcctcgcat gagggcgcac gagcttgcac ggttggcgga ggaactcggg
     4801 ctcgacgcga tcggcgcgac cccggcacgg ccctacgagg agaccgagcg gcacattcgc
     4861 gaacgacgcg caaagggcct tttcgcggag atgcgcttca caatggccca gccggaggtc
     4921 tcctgtcatc cggagaagct cttccccgga gccgctaccg tcgtctccgc cgcgctctgc
     4981 tactacgtgc ccggcggcga gccgggtctc ggcgaggggc ggctcccccg ctacacgtgg
     5041 cgggatcact acgccctgtt gcgcgagcgg ctctcggctc tcggcgagcg gctgggcggc
     5101 gagtaccgcg tgctcgtcga cgccaatcag cacgtcgacc gggagggagc tgcgcgaggc
     5161 ggcgtcgggt tctacggcaa gaacaccatg ctgatcacac cgtcgcacgg ctcctgggtc
     5221 gtgctcggaa ccctcgtcac cgacgtggcg atcgagccca cgtcacctgt cgagctcgac
     5281 tgcggctcct gccggctgtg catcgacgcc tgccccaccg gtgcgctcga cgatcccggt
     5341 gtgctcgact cgacgaagtg catcgcctac tgggcgcagg cgccgcgccc gatcccgtcg
     5401 gagtaccggt tcgagctcgc ccagaacgcg tacggatgcg acgtgtgcca ggacgtctgc
     5461 ccgtggaacc acccgatcga gaaacgccga gcggactcag ccctt
//
LOCUS       DBNGNK010000234         7940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_186399,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000234 DBNGNK010000000
VERSION     DBNGNK010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_186399"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="AC150V_06920"
     CDS             complement(<1..353)
                     /locus_tag="AC150V_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR3762691.1"
                     /translation="MTVERPLRIAVVGAGPAGFYAAGALLAADPPVEVDVLDRLPTPW
                     GLVRTGVAPDHPNIKAVSRAFEKIAQRPGFRFLGNVEVGRDVTHSELAELYDGIVYSY
                     GAETDRSMGIPGEDLP"
     gene            complement(398..817)
                     /gene="erpA"
                     /locus_tag="AC150V_06925"
     CDS             complement(398..817)
                     /gene="erpA"
                     /locus_tag="AC150V_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010025363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR3762692.1"
                     /translation="MEIAERPTETLLTLTPVAAEKIKELLAEEQDSGTMVLRVAIQGG
                     GCSGFQYGLGFDTGAMDSDLELELEGVPVVVDPYSAPYLQGATIDFLNTIQESGFKIE
                     NPNAVSSCGCGHSFQVDEGEELPEGTQMGGCGSGCGA"
     gene            1032..1334
                     /locus_tag="AC150V_06930"
     CDS             1032..1334
                     /locus_tag="AC150V_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR3762693.1"
                     /translation="MITGSFVFATRPRTRTKQRMIPFESGVSEGAPPMERRFTVSFYL
                     TAMLFILFDVEIVFLYPLAVQLDLLGWFGLVEFLVFIGILAVAYVYIWRKGALQWR"
     gene            1448..1927
                     /locus_tag="AC150V_06935"
     CDS             1448..1927
                     /locus_tag="AC150V_06935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR3762694.1"
                     /translation="MEEALGLTTVEKAIAWAQSSSVWPDTFGLACCAIEMMSIVSSRY
                     DIARFGMERFSSSPRQADLLIVSGRVTHKMARPLRQVYDQMLEPKWVIAMGACSSSGG
                     MFNNYTVLQGVDKIVPVDIHVPGCPPRPEALMDGIVRLHEKIRAGVPPAYMIRGVGE"
     gene            1941..2486
                     /locus_tag="AC150V_06940"
     CDS             1941..2486
                     /locus_tag="AC150V_06940"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR3762695.1"
                     /translation="MAYESVPGLVETTEAYGETTLVVDPARLVEACVHLRDEEGFSFL
                     SDISPTDYLGWGEQGVAGYIGTAEGRNINAPGSHGQQRVPDPKPKRFSVSYHLLRLAD
                     APVRVRVKVWLDDGEDVDSVIDVWPAADWFEREAWDMFGIRFGGHPNLRRILLDDDWE
                     GHPLRKDYPLGGEPVRFSGEE"
     gene            2564..3760
                     /gene="nuoD"
                     /locus_tag="AC150V_06945"
     CDS             2564..3760
                     /gene="nuoD"
                     /locus_tag="AC150V_06945"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR3762696.1"
                     /translation="MDPSKHDPEDILTINFGPNHPSTHGVLRLIVELDGEQVAGISAV
                     IGYLHTGFEKNMEHKTYWKAITYPERIDYLSFQANELVFVLAIEELLGIAVPRKATWT
                     RMCLAELNRIHSHLVWLATGALELGAISMFWYAFRERDMVLDLFEMVTGVRMHTRYFQ
                     AGGLAEDVPRGFIPEARAFCERMPKAVDEYEAILDRNQIWLERTKGIGLLSAEDAIAL
                     GQSGPMLRASGVDWDLRRAAPYLAYEEVDFRVPVYPEGDVYARYKVHMDEMRESTRIV
                     SQCLDRLEEMEGEPWIADDRKVVLPPRHELHTSMESLIHHFKIVTEGYRVPEGEVYVT
                     IESPRGECGCYLVSDGGTKPWRVKFRAPSFAALQATATVVQNAAIADLIAIAASLDPV
                     MGDVDR"
     gene            3757..4230
                     /locus_tag="AC150V_06950"
     CDS             3757..4230
                     /locus_tag="AC150V_06950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR3762697.1"
                     /translation="MSALYDEIQKLRDVYPERRSAILPALHLAQEEHGGWLPADAFRE
                     VADALDVSAAYCYSIASFYDMFRLEPAARVTVEVCTNISCAARGAQQVLEAFERELGV
                     RAGEKSADGEFELGTIECLGGCGWATVISVNERQRTGVQPGDVAAIAEELRNGSG"
     gene            4217..5557
                     /gene="nuoF"
                     /locus_tag="AC150V_06955"
     CDS             4217..5557
                     /gene="nuoF"
                     /locus_tag="AC150V_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005184260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR3762698.1"
                     /translation="MAAAEASQIVFAGTDGGAVTELQQYLDRGGFTALEKARAMSPEE
                     LIAEISTSDLRGRGGAAFPTGRKWSFVPKPDQLPKPHYLVVNADESEPGSFKDREIML
                     RVPFRFVEGCLISAYANEASIVFVYIRGEYDEAYDVLRSALEQLDGRPDLLGGVKIVL
                     HRGAGSYICGEETALIESLEGWRGQPRTKPPFPAVAGLYASPTVVNNVESLATVVPIV
                     EMGAAEYARIGVENSTGTRVFSLSGNVVNGGNYELVSGTTLRELIFEHGGGIAGGRSL
                     KAICPGGLSTPILSADQVDTPLDIMSLAAAGTAIGSAGVIALDDRCCMVEYALRASRF
                     YEHESCGKCTPCRVGTRWLSQILQAIEDGEGTATDLDLLLDVAERVNGKCLCPLGDSD
                     ALVVASCVDKFRDEFQAHIELGRCPFDDSPLAGVLAPAVQVLRRLHRELDVIPA"
     gene            5554..7800
                     /gene="nuoG"
                     /locus_tag="AC150V_06960"
     CDS             5554..7800
                     /gene="nuoG"
                     /locus_tag="AC150V_06960"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HIR3762699.1"
                     /translation="MSELVSVKIDDREVRVAKGTGLIETAAEAGIEIPVFCYEPRLGA
                     PVGACRMCLVEVEGMPKLQAGCTLTAQDGMIVRTAETSEQAADGQNATLEFILVNHPL
                     DCPVCDKGGECPLQDLTFRYGPGHTRMTFAKQTFDKPIPISPTIALDRERCILCYRCT
                     RFSSDVAEDEQLVAINRGAHSLIATFEDEPYRAPFSGNVIELCPVGALTSTQYRFEAR
                     PWEIQDVPSVCGLCPVGCNISATTREGKVKRVISRNHPEIDRGWLCDKGRFTYPHLYA
                     DDRVLHPLARRPRTGFAEVSWDEAVDEAERLLREAGGKIVTALSGSETIEQAYALGRL
                     LRAGLGAHSAVLPESTSSALDAFRAPLSTIAEAELIVVVGDEAVDDRAPIVDLWIRQA
                     VRAGAEAITASPTGSAPVEPGSAAQVLRAIAGEPGSVAPPGIDAVGLGNKLRAADRAV
                     LIWSGPHGGGGGRLAEAAHALGFESKPGCAAFHLPATPNGRGVAEAWAASAEGDEANP
                     EPIALLVVSGDTAAADPSVRRLAEHADRVIAITMFHDLAVGWADLVLPATSYLEQDGT
                     MLNLEGRLQRLRRTVLPPVPDELAWISTLAARFDVSVSPYASAVFEEVSTLAFGGVTG
                     DEIGEQGTVTGRAPYVAPQPAPETPTVAAQPQGIALLRYRPLFSGPHVERVDEVSFQR
                     PERVLELAPGDAGPRGIAAGDTVVATSNGTSLDLTARIRRTLHEGVARIAEEHAGDLQ
                     GSIEVEKR"
BASE COUNT         1260 a         2706 c         2692 g         1282 t
ORIGIN      
        1 ggcagatctt cgcccggtat gcccatgctg cgatcggtct cggcgccgta cgagtagacg
       61 atgccgtcgt acagctcggc gagctcggag tgcgtgacgt cgcgccccac ctcgacgttg
      121 ccgagaaaac gaaagcccgg ccgctgcgcg atcttctcga acgcccgcga gacggccttg
      181 atgttcggat ggtcgggggc gacgccggtt cgaacgagcc cccatggagt cggcaggcgg
      241 tcgagcacgt cgacctcgac gggcggatcg gccgcgagaa gcgccccggc agcgtagaag
      301 ccggccgggc cggcgccgac gacggcaatg cggagcggac gctcgacggt cacgcgacga
      361 tcctagagct tggtgttcgg acgaacgctg aggtctgtca ggcgccgcaa ccggagccgc
      421 agccgcccat ctgcgtgccc tccggcagct cttcgccctc gtcgacctgg aaggagtggc
      481 cgcagccgca cgacgagacc gcgttcgggt tctcgatctt gaagccggac tcctggatcg
      541 tgttgagaaa gtcgatcgtg gcgccctgga ggtacggcgc gctgtagggg tcgacgacca
      601 ccgggacgcc ctcgagctcc agctcgaggt cactgtccat ggcgcccgtg tcgaagccca
      661 ggccgtactg gaagccggag caaccgccac cctgaatggc cacgcgaagc accatcgtgc
      721 cgctgtcctg ctcctcggcc aggagctcct tgatcttctc cgcggcgacg ggtgtcagcg
      781 tgagcagggt ctcggtcggg cgctctgcga tctccacgct ctgatcgtag ccgaacgggt
      841 ggcggatgcg gagagaacct gagcttgtaa cagcagtttc tctttcgtcg tgcgggtttg
      901 ttgcctgcct cgtgcggggt agtgaaggtg ctaggtttcg cgcagccgtc ccgctgggaa
      961 aggaatgcgt gctcgctgac tggctgccat ttctgatcta cgcgcttttc gcagcagcta
     1021 tcccggcgac gatgatcacg ggctcgttcg tgttcgcgac gcggccgagg acacggacga
     1081 agcagcgcat gatcccgttc gagtccggtg tctccgaggg cgccccgccg atggagaggc
     1141 gcttcacggt cagcttctac ctgacggcga tgctcttcat tctcttcgac gtcgagatcg
     1201 tgtttctcta cccgctcgcg gttcagctcg atctgctcgg ctggttcggg ctcgtcgagt
     1261 tcctggtctt catcggcatc ctcgccgtgg cgtacgtcta catctggcgg aagggggcac
     1321 tccagtggcg gtgaagcgag cgcttcccct gctcagcgac cgcccactcg cgaacgccgg
     1381 cctcagctcg gagcgggttc tctggcccga caagggtccc ggcgtgttcg agcaggaggt
     1441 cgagaacatc gaagaggccc tcggtctgac aacagtcgag aaggcgatcg cctgggcgca
     1501 atcgagctcg gtctggcccg acaccttcgg tctcgcctgt tgtgccatcg agatgatgtc
     1561 catcgtctcg tcgcgctacg acatcgcgcg tttcggcatg gagcgcttca gctcgtcgcc
     1621 gcggcaggcc gacctgctga tcgtctcggg acgggtgaca cacaagatgg cccgcccgct
     1681 ccggcaggtg tacgaccaga tgctggagcc gaagtgggtg atcgcgatgg gcgcctgctc
     1741 ttcgagcggc ggcatgttca acaactacac ggtgctccag ggcgtagaca agatcgtccc
     1801 ggtcgacatc cacgtacccg gctgtccacc gcgccccgag gcgctgatgg acgggatcgt
     1861 gcgactgcac gagaagatcc gcgccggcgt tcctcctgcc tacatgatcc gcggagtcgg
     1921 cgagtgaggt cgaccggcta gtggcatacg agagcgttcc gggccttgtc gagacgacgg
     1981 aggcttacgg agagaccacc ctcgttgtcg acccggcacg gctcgtcgag gcgtgcgttc
     2041 acctccgcga cgaggagggg ttctcgtttc tctccgacat ctcgcccacc gactatctgg
     2101 gctggggcga gcagggcgtc gcaggctaca tcggcacagc tgagggccgc aacatcaacg
     2161 ctcccggctc gcacggccag cagcgcgtgc ccgacccgaa gcccaaacgc ttctcggtca
     2221 gctaccacct cctcaggctc gcagacgccc ccgtacgcgt tcgcgtcaag gtctggctcg
     2281 acgacggcga agacgtcgac tcggtgatag acgtctggcc cgcagcagac tggttcgagc
     2341 gcgaggcctg ggacatgttc gggatccgct tcggcggaca cccgaaccta cgccgcatcc
     2401 tcctcgacga cgactgggaa gggcacccac tgcggaagga ctatccgctc ggcggcgagc
     2461 ctgtgcgctt ctcgggggag gagtagccgt gtcggtactg gagcgcacgc gaaagctcta
     2521 cgaaggatcg cggatcccgc ggccgacacc cacggtactg aagatcgacc cctcgaagca
     2581 cgacccggag gacatcctca cgatcaactt cgggccgaat cacccctcga cgcacggcgt
     2641 gctgcgcctg atcgtcgagc tcgacggcga gcaagtggcc ggcatcagcg cggtcatcgg
     2701 ctacctccac actggcttcg agaagaacat ggagcacaag acctactgga aggcgatcac
     2761 ctacccggaa cggatcgact acctctcctt ccaggcgaac gagctcgtgt tcgtgctcgc
     2821 aatcgaggag ctgctgggta tcgccgttcc gcgcaaggcg acgtggacgc ggatgtgcct
     2881 cgcggagctg aatcgcatcc actcgcatct cgtctggctc gccaccgggg cgctcgagct
     2941 cggtgcgatc tcgatgttct ggtacgcgtt ccgcgagcgc gacatggtgc tcgacctgtt
     3001 cgagatggtc accggcgtgc gcatgcacac gcgctacttc caggccggcg gtctggctga
     3061 ggacgttcct cgcggtttca ttccagaggc tcgggccttc tgcgagcgga tgccgaaggc
     3121 cgtcgacgag tacgaggcga tcctcgaccg caaccagatc tggctcgagc gaacgaaggg
     3181 gatcggtctc ctctccgccg aggacgcgat cgcgctcggg cagtctggcc cgatgctccg
     3241 cgcctcgggc gtcgactggg atctgcggcg ggcggctccc tacctcgcct acgaggaggt
     3301 cgacttccgc gttcccgtgt atccggaggg cgacgtgtac gcccgctaca aggttcacat
     3361 ggacgagatg cgcgagtcga cacgcatcgt tagccagtgc ctcgaccggc tcgaggagat
     3421 ggagggcgag ccctggatcg ccgacgaccg caaggtcgtc ctgccgccca gacacgagct
     3481 ccacacgtcg atggagtcgc tgatccacca cttcaagatc gtcaccgagg ggtaccgcgt
     3541 gcccgagggc gaggtctacg tcacgatcga gtccccgcgc ggcgaatgcg gctgctacct
     3601 cgtctcggac ggcggcacga agccctggcg cgtcaagttc cgagcgccga gcttcgcggc
     3661 gcttcaggcg actgcgacgg tggtgcagaa cgcggccatc gccgacctga tcgcgatcgc
     3721 ggcctcgctc gacccggtga tgggggacgt cgaccgatga gcgcgctcta cgacgagatc
     3781 cagaagctcc gcgacgtcta tccggagcga cgctcggcga tcctgccggc gctgcacctc
     3841 gctcaggagg agcacggcgg ctggctgccc gccgacgcgt tccgagaggt ggccgacgct
     3901 ctcgacgtgt cggccgcgta ctgctactcg atcgcgagct tctacgacat gttccggctc
     3961 gagcccgcgg cgcgcgtgac ggtcgaggtg tgcaccaaca tctcgtgcgc cgcgcgaggg
     4021 gcacagcagg tgcttgaggc cttcgagcgc gagctgggcg tgcgggccgg cgagaagagc
     4081 gcggacggcg agttcgagct cggcacgatc gagtgcctcg gcggctgcgg ctgggccacc
     4141 gtgatctccg tcaacgagcg tcagcggacc ggcgtccagc ccggagacgt tgccgcaatt
     4201 gcggaggagt tgcgcaatgg cagcggctga ggcctcccag atcgtcttcg ccggaaccga
     4261 cggcggcgct gtgaccgagc tgcagcagta cctcgaccga ggcggtttca ccgcgctcga
     4321 gaaggcgcgc gccatgagcc ccgaagagct gatcgcggag atctcgacca gcgatctgcg
     4381 cggccgtggc ggtgccgcat ttcccaccgg gcgcaagtgg agcttcgtgc ccaagccgga
     4441 ccagttgccc aagccccact acctcgtcgt gaacgccgac gagtcggagc cgggctcgtt
     4501 caaggatcgc gagatcatgc tgcgcgtgcc gttccgcttc gtcgagggct gcctcatctc
     4561 cgcctacgcg aacgaggcct cgatcgtgtt cgtctacatc cgcggcgagt acgacgaggc
     4621 ctacgacgtg ctccgctcgg cactggaaca gctggacggg cggccggacc tgctgggcgg
     4681 agtcaagatc gtcctgcacc gcggcgcggg ctcctacatc tgcggcgagg agacggcgct
     4741 gatcgagtcg ctcgagggct ggcgtggcca gccgcgcacg aagccaccgt tcccggccgt
     4801 cgccggcctc tatgcgtcgc cgaccgtcgt gaacaacgtc gagtcgctgg cgacggtggt
     4861 gccgatcgtc gagatgggtg cggcggagta cgcgcgcatc ggggtcgaga actcgaccgg
     4921 gacacgggtc ttctccctgt cgggcaacgt cgtcaacggc gggaactacg agctcgtcag
     4981 tggcacgacg ctgcgcgagc tgatcttcga gcacggcggc gggatcgcgg gcggtcgttc
     5041 gctgaaggcg atctgtccgg gcgggctctc gacccccatc ctgagcgccg atcaggtcga
     5101 tacgccgctc gacatcatgt cgctcgccgc ggcgggcacg gcaatcggct cggccggggt
     5161 gatcgccctc gacgatcggt gctgcatggt cgagtacgcg ctgcgcgctt cgcgcttcta
     5221 cgagcacgag tcgtgcggga agtgcacgcc gtgccgtgtc ggcacgcgtt ggctctccca
     5281 gatcctccag gcgatcgagg acggcgaggg caccgccacc gacctcgatc tcctgctcga
     5341 cgtcgcggag cgggtgaacg gcaagtgcct ctgcccgctc ggcgattcgg acgcgctcgt
     5401 cgtcgcgagc tgtgtcgaca agttccgcga cgagttccag gcgcacatcg agctcggacg
     5461 ctgtccgttc gacgactccc cgctggcagg cgtgctggcg ccggctgtcc aggtgctacg
     5521 ccgtctccat cgcgagcttg acgtgatccc cgcatgagcg agctcgtctc cgtcaagatc
     5581 gacgatcgag aggtgcgggt cgcgaagggc accggcctga tcgagacggc ggccgaggcc
     5641 gggatcgaga taccggtctt ctgctacgag ccgcggctcg gcgcccccgt cggcgcctgc
     5701 cgcatgtgcc tggtcgaagt cgagggaatg ccgaagctcc aggccggctg cacgctgacg
     5761 gcccaggacg ggatgatcgt cagaacggcc gagacgtcgg agcaggcggc cgacggccag
     5821 aacgccaccc tcgagttcat cctcgtcaac cacccgctcg actgccccgt ctgcgacaag
     5881 ggcggcgagt gcccgctgca ggacctcaca ttccgctacg ggccgggaca cacgcgaatg
     5941 acgttcgcga agcagacctt cgacaagccg atcccgatct cgccgaccat cgccctcgac
     6001 cgcgagcgct gcatcctctg ctaccgctgc acccgcttct ccagcgacgt cgccgaggac
     6061 gagcagcttg tcgcgatcaa ccggggtgcc cactcgctga tcgccacctt cgaggacgag
     6121 ccctacagag cgccgttctc cggcaacgtg atcgagctct gcccggtcgg cgcgctcacc
     6181 tcgacgcagt accgcttcga ggcccgtccg tgggagatcc aggacgttcc aagcgtgtgt
     6241 ggcctctgcc cggtcggctg caacatctcc gcgacgacgc gcgagggaaa ggtcaagcgt
     6301 gtgatctcgc gcaaccaccc ggagatcgac cgcggctggc tctgcgacaa aggccgcttc
     6361 acgtacccgc acctctacgc cgacgaccga gtcctgcacc cactcgcgcg ccgcccgcga
     6421 accggcttcg cggaggtgtc ctgggacgag gccgtcgacg aggcggagcg tctgcttcgc
     6481 gaagcgggcg gcaagatcgt gaccgcgctc tcgggctccg agacgatcga gcaggcgtac
     6541 gcgctgggcc gcctgctccg tgcgggcctg ggcgcccact cggccgtgct gcccgagtcg
     6601 acctcgtcgg cccttgacgc gttccgtgcg ccgctctcga cgatcgcaga ggccgagctg
     6661 atcgtcgtcg tcggcgacga ggcggtcgac gaccgggcgc cgatcgtcga cctctggatc
     6721 cggcaggcgg tgcgggcggg cgccgaggcg atcaccgcca gcccgaccgg aagcgctccg
     6781 gtagagcctg gatcggccgc ccaggtgctc cgcgcgatcg ccggcgaacc aggctccgtc
     6841 gcgccccccg gcatcgacgc cgtagggctg ggcaacaagc tgcgcgcagc cgaccgggca
     6901 gtcctgatct ggtccggccc ccacggcggc gggggcggcc ggctcgcgga ggccgcgcac
     6961 gcgctcggat tcgagagcaa gccgggctgc gcggcgttcc atcttccggc tacaccgaac
     7021 ggtcgcggcg tcgccgaggc ctgggcggcc tccgccgagg gggacgaagc caatccggag
     7081 cccatcgccc tgctcgtcgt ctcgggagac accgccgccg ccgatcccag cgtgcgtcgg
     7141 ctcgccgagc atgctgaccg cgtcatcgcg atcacgatgt tccacgacct cgcggtgggc
     7201 tgggcggacc tcgtgctccc ggccacgagc tacctcgagc aggacggcac gatgctaaac
     7261 ctcgaagggc gcctgcaacg gctgcggcgc acagttctcc cgcccgttcc ggacgagctg
     7321 gcgtggatct cgacgctggc ggcacgcttc gacgtgtcgg tgtcaccgta cgcctcggct
     7381 gtcttcgagg aggtctcgac actcgcgttc ggcggtgtca caggcgacga gatcggagag
     7441 cagggcacgg tcacgggtcg tgcgccgtac gttgcaccgc agcccgcacc cgagacaccc
     7501 accgtcgccg cgcaacctca ggggatcgcg ctgctgcgct accgacccct cttctccggg
     7561 ccgcacgtcg agcgcgtgga cgaggtctcc ttccagcgcc cggagcgcgt cctcgagctc
     7621 gcgcccggcg acgccgggcc gcgcggaatc gcggccggcg acacggtcgt tgccacatcg
     7681 aacggcacgt cactcgacct gacagcgcgc atccgccgca cgctgcacga gggcgttgcg
     7741 cggatcgcag aggagcatgc cggcgacctg caaggctcga tcgaggtcga gaagcgttga
     7801 gctggctgct ggcacagaac ggcgcgaacg gcgagccgtg gtggatcgcg ctgatcaagg
     7861 cggccgtgat catcaacctg gtgctggcaa ccttcgccta tctcacactg accgagcgca
     7921 aggtgatggg ccgtatgcag
//
LOCUS       DBNGNK010000235         4474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_115046,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000235 DBNGNK010000000
VERSION     DBNGNK010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_115046"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="AC150V_06965"
     CDS             <1..2115
                     /locus_tag="AC150V_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3762700.1"
                     /translation="TIGLAPLSGAEIAELISALLEQAVLPADTQRLIQERAGGNPLYA
                     EEFVRMLRDRELVDSRGALVEGADVPFPDSLQALIAARLDTLSTERKGLLQDAAVVGK
                     VFWSGAVAAIGERDAREVEIALHELARKELVRPSRQSSMEGEQEVGFWHALVRDVAYG
                     QIPRAQRAAKHVAAARWIEGKAGDRVEDLADVLAHHTITAVELAEAKGDAALAADTAP
                     AARRYALLAGERALGLDTAKAVELLERAVELTPEGDPAYAGAQVRFAIAAHQAGRREA
                     AGAALETAIGDLRARGDVLQRGEALTLLALIAPGDPSRNVLFCLEAVELLESAPPGPE
                     LVAAYAQLSGHRLVRGQYGESLENSERALTLARELGLPIPARALGFHGSALCNTGRGA
                     AAGLAEQEEARSLLIAEGRGRDAAVATHNLAIARWTLEGPVAATRTLQEAVSFAAERG
                     LLEAQVASLASGLERLTDAGRFDEVLAVASDLAALTERTIPTALNAVEAAAAVSRVHH
                     ERGDAATAALSADDALAAARRGGLPIAVAIAAGPSAATRLAQSDAAAARELLGEVASW
                     QDGPANEEYLSRLPAYVRYAIAADDLVLADDLIRKVRAVLPLHEHALVAARALIAEAR
                     GEQAQAADRFAEAASRWEGFGAVLEQAYALLGQGRSLVSLGDPAAERPLRAARALFDE
                     MGARPRVADCDALLSEALAAGA"
     gene            2158..3810
                     /locus_tag="AC150V_06970"
     CDS             2158..3810
                     /locus_tag="AC150V_06970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR3762701.1"
                     /translation="MSDSTLRIIPLGGLGEVGKNMTVYELGDEIIVVDSGLAFPRDEH
                     LGVDLVLPDFAYLRDKRIRAIVLTHGHEDHVGGLPYALRELDVGEVVATRLTLGLIKS
                     KLDEHGLGAATELRELTPEQGPTDIGPFRVEPVRMAHSIPDAVALVIECAAGRIVHTG
                     DWKLDHTPVDGLRTDVGKLAEIGNRGVDLLLGDSTNAERAGMTGSERLVGEAFQQLIP
                     VRKGRILVATFGSQIHRMQQAADVAAQSGRKVAIVGRSMRKNMNIARNLGYVEIAEDS
                     IVSAREAMELPPERALILCTGSQGEPMSALTRVAFDDHATISVERGDTVIISAKPVPG
                     NELRVHDAINRLARSGAEVLHEEIAPVHVSGHANAEELRTLLALLRPKAVMPIHGEYR
                     MLAAHARLAQDAGISSDSIILADNGSLVELDAEGARLAGRVESGVTFVDGLRVGDVQD
                     VALRDRRHLSEDGVLIVVTTVSPTNGGEIAPPELIARGLAEDDELLQELRAEADRITA
                     NLAESGMTEIKLLQEHLHDEIGQLVYDRTRRRPMILPVVIEV"
     gene            3812..4348
                     /locus_tag="AC150V_06975"
     CDS             3812..4348
                     /locus_tag="AC150V_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762702.1"
                     /translation="MSGWPDVIAENAANELTARRLAGQLRACETAAIAFCRLLERWNK
                     GEARPATAGAREAALRRAADRVETALAGLDEPLSRYLLELEPERAEGSSWYGGPGAGE
                     LVEWRPVLDRAGVHACPNRVASVYLELALLVRALQGLDDAARLEMSPDRSSLWAGLFD
                     LRDTLFPSTVDDLRALAA"
BASE COUNT          705 a         1564 c         1546 g          659 t
ORIGIN      
        1 acgatcggtc tcgctccgct ctcgggcgcc gagatcgccg agctgatcag cgcgctgctc
       61 gagcaggctg tactccctgc ggacacgcag cggctgatcc aggagcgcgc cgggggcaac
      121 ccgctctacg cagaggagtt cgtgcgcatg ctgcgcgacc gcgagctcgt cgattcgaga
      181 ggcgctctgg tcgagggcgc ggacgtgccc ttccccgact cgttgcaggc gctgattgcc
      241 gcgcggctcg acacgctctc caccgagcga aagggactgc ttcaggacgc ggccgtggtc
      301 ggcaaggtct tctggtcggg cgcggtcgca gccatcgggg agcgcgacgc gagagaggtg
      361 gaaatcgctc tccacgagct ggcccgcaag gagctcgttc gcccctcgcg gcagtcgtca
      421 atggaaggcg agcaggaggt cggcttctgg cacgcgctcg tacgagacgt cgcctacgga
      481 cagatcccgc gggcccaacg agcggcaaag cacgtagcgg ccgcccgctg gatcgaggga
      541 aaggcgggtg accgggtcga ggacctcgcc gatgtcctcg cccaccacac gattacggcg
      601 gtcgagctgg ccgaggcgaa gggtgacgca gctctcgccg ccgatacagc cccggcagca
      661 cggcgctacg cgcttctcgc cggcgagcgg gcgctcggcc tcgataccgc gaaggccgtc
      721 gagctactgg agcgcgcagt cgagctgacg cctgagggcg accccgcgta cgcaggcgcc
      781 caggtgcgct tcgcgattgc cgcacaccag gcaggacggc gagaggcggc gggagccgca
      841 ctcgaaactg caatcggcga tctccgcgct cgaggagacg ttctgcagag gggtgaagca
      901 ctgacgctgc tcgctctcat tgcccccggc gacccttcac gcaacgtcct cttctgcctc
      961 gaggccgtcg agctgctcga gtcagcgcca ccgggcccgg agctcgttgc ggcctacgcg
     1021 cagttgagcg gccatcgact ggtgcgcggc cagtacggcg agtcgctcga gaactcggag
     1081 cgtgcgctca cgctggcccg ggagctcggc cttcccatcc ctgccagggc actcggcttt
     1141 cacggcagtg ctctgtgcaa cacgggcaga ggagcggctg ccggcctcgc cgaacaggag
     1201 gaagcccgtt cgctcctcat cgccgagggc cgcggacgag atgcggccgt cgcaacgcac
     1261 aacctcgcaa tcgcccgctg gacgctcgag gggccggttg cagcgactcg aacgctgcag
     1321 gaagcggtgt cgtttgcggc agagcggggg ctgctcgaag cccaagtcgc gtcgctcgcc
     1381 tcagggctcg agcgtctcac cgacgccgga cggttcgacg aagtccttgc cgtcgcgagt
     1441 gacctggctg cattgactga gcggaccatc ccgaccgcac tcaacgctgt cgaggccgca
     1501 gcagccgtca gtcgcgttca ccacgagcgc ggggacgctg caaccgccgc gctctcggcc
     1561 gacgacgccc ttgcggcggc gcgtcggggc gggctgccga tcgccgtggc gatcgccgct
     1621 ggcccttccg ccgcgacgag gctcgcccaa agcgacgccg ccgcggcgcg cgagctgttg
     1681 ggcgaggtcg catcctggca agacggcccg gccaacgagg aatacctttc gaggctcccc
     1741 gcctatgtcc gatacgccat cgcggcggac gatctcgtgc tcgccgatga cctcatccga
     1801 aaggtcaggg ccgtcttgcc gctgcacgag cacgccctcg tcgccgcgcg tgcgctgatc
     1861 gcggaggccc gcggcgagca ggcgcaagca gctgatcgtt tcgccgaggc ggcttcacgg
     1921 tgggaaggct tcggcgccgt gctcgagcag gcctacgctc tgctcggcca gggtcgctcc
     1981 ctcgtctcgc tcggcgaccc ggccgcagaa cggccgctga gagccgcccg ggccctcttc
     2041 gacgagatgg gtgcgcgacc gcgcgttgcc gattgcgacg cgctgctctc ggaagcgctc
     2101 gccgccggcg cctgacaagc gcctgcatgc cggcgcccgg cactacactc cgaccggatg
     2161 tcggactcga cattacgcat cattccgctc ggcggcctcg gcgaggtcgg caagaacatg
     2221 acggtctacg agctcggcga cgagatcatc gtcgtcgact cgggcctcgc gtttcctcgt
     2281 gacgagcatc tcggcgtcga cctagtcctg ccggacttcg cgtaccttcg cgacaagcgg
     2341 atacgcgcca tcgtcctcac gcacggccac gaagaccacg tcggcggtct gccgtatgcg
     2401 ctgcgggagc tcgacgtcgg cgaggtcgta gcgacgcgac tgacgctcgg actgatcaag
     2461 tccaagctcg acgagcacgg actgggtgcc gcgaccgagc tccgcgagct cactccggag
     2521 cagggcccaa ccgacattgg gcctttccgc gtcgagccgg tgcggatggc ccactcgatc
     2581 cccgacgcgg tcgcgctcgt gatcgagtgc gcggccggac gcatagtcca tacgggagac
     2641 tggaagctcg accatacgcc ggtggacggg ctgagaaccg acgtcggcaa actggccgag
     2701 atcggcaatc gcggcgtcga cctcctgctc ggtgactcca cgaacgccga gcgggccggc
     2761 atgaccggat ccgagcggct ggttggcgag gcgttccaac agctgatccc ggtccggaag
     2821 ggccggatcc tcgtggccac gttcggctct cagatccacc ggatgcagca ggccgccgac
     2881 gttgcagccc agagcgggcg caaggtcgcg atcgtcgggc gctcgatgcg caagaacatg
     2941 aatatcgcgc gcaacctcgg ctacgtcgag atcgccgagg attcgatcgt ctcggcgcgc
     3001 gaggcgatgg agctgccacc ggagcgcgcg ctcatcctgt gcaccggaag ccagggcgag
     3061 ccgatgtccg cgctgacgcg ggtcgccttc gacgaccatg cgacgatctc cgtcgagcgg
     3121 ggtgacacgg tgatcatctc ggcgaagccg gttcccggga acgagcttcg tgtccacgac
     3181 gcgatcaacc ggctggcccg ctccggcgcc gaggtgctgc acgaggagat cgcacctgtg
     3241 cacgtctccg gccacgccaa cgccgaagag ctccgcacgc tgctcgcctt gctccggccg
     3301 aaagcggtga tgcccataca cggcgagtac cggatgctcg cggcgcacgc acggctcgca
     3361 caggacgccg ggatctcgtc cgactccatc atcctcgccg acaacggctc gctcgtggaa
     3421 ctggacgccg agggagcgag gctcgccggc agggtcgagt cgggcgtcac cttcgtcgac
     3481 gggctgcggg tcggcgacgt ccaggacgtc gccctgcgcg accgacggca cctgtcggag
     3541 gacggtgttc tcattgtcgt gacgactgtc tccccgacca acggcggcga gatcgccccg
     3601 cccgagctga tcgcccgggg tctcgccgaa gacgacgagc tgctccagga gcttcgggcc
     3661 gaggccgacc gcatcactgc gaacctggcg gaatccggca tgaccgagat caagttgctc
     3721 caggagcatc tgcacgacga gatcgggcaa ctggtctacg atcgcacgcg gcgtcggccg
     3781 atgattctcc cagtcgtcat cgaggtgtga catgagcggc tggcccgacg tcatcgccga
     3841 aaacgcagcc aacgagctca ccgcccgccg cctggcgggg cagctgcgcg cctgcgagac
     3901 ggcggcgatc gcgttctgtc gcctgctcga gcgctggaac aagggggagg cgcggccggc
     3961 cacggccggt gcccgtgaag ctgcgctacg ccgggccgcg gaccgagtgg agactgcact
     4021 cgccgggctc gacgagccgc tgtcgcgcta tctcctcgag ctcgagcccg agcgggcgga
     4081 gggcagctcg tggtacggcg gtccgggggc gggcgaactg gtcgaatggc gcccggtgct
     4141 cgaccgtgcg ggcgtgcatg cctgtccgaa ccgagtcgcg tccgtctacc tcgagcttgc
     4201 gctcctcgtc cgagccctcc agggtctcga cgatgcggcg cgcctcgaga tgagcccaga
     4261 ccgctcgtcg ctgtgggccg ggctgttcga ccttcgggac acgctctttc cctcgacggt
     4321 cgacgacctg cgcgcacttg ccgcgtgacg gctgcgaatc gagagcaccg cgacgagctg
     4381 ctcgaggagg tgctcgagcg atactcgggc tcgccaaacc cgcgcctccg cgagatcacc
     4441 gaggcagcaa tccggcatct acacgcgttc gccc
//
LOCUS       DBNGNK010000236         2082 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_903454,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000236 DBNGNK010000000
VERSION     DBNGNK010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_903454"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1416
                     /locus_tag="AC150V_06980"
     CDS             172..1416
                     /locus_tag="AC150V_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3762703.1"
                     /translation="MVIGERFAHLVPAAADLTDPAYQTAVRTLADELADTLGSAAAAR
                     ELESELVAAVEPVPIPLRVALKLAMSRLTLAALTQPVRLSVVFAVYKEHLRILRSEEN
                     EHGEDFLRQKLAQLDWLCADTRHAWDLTVVDDGCPEGSGEIARRIVEELDVGNRVTVL
                     FLADAIRDGLPAAGGLTSTDGSRKGGSIRFGLWHALQTPHPTHVLLYTDADLSTDLAQ
                     AGLLVEPLVADGRTAASGSRRRPESVVVKGAGRNVRGKLFIYLWKRLIPQLRGVVDTQ
                     CGFKAFGADGLRRWLLETSENGFAFDIEYLLRVELDSPGSLARVPIAWIDSEALSTTT
                     GLQPYPAMLRSVVALYRRYEEPTGDAESFASLIDSLDDAAFERLLGAVPPAIADRDPH
                     EFDEFADVAAAELAEIAFGKTR"
     gene            1513..>2082
                     /locus_tag="AC150V_06985"
     CDS             1513..>2082
                     /locus_tag="AC150V_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HIR3762704.1"
                     /translation="MPSLPRLSRRRRSRGSEVATRSAAEVRELTASGLSWVNAVSPDL
                     DAAQELADRFGWHPLDVEDIVSKRQRPKVDDYDEEGYLFIVLHFPVFDKAIQRLNAAE
                     IDIVLGHDYLVTLPNRELLPVTRLFARCEEDADFREALFERGSGRLFYEVLDDLFDYC
                     FPILDKIAHKLDRIEDDVFEGRSDDVVRDI"
BASE COUNT          343 a          721 c          681 g          337 t
ORIGIN      
        1 agcagcagcg cgatcttcac aaacgccttc atagatgctc ccctcttgta agcctcgaag
       61 aacgacgcgc cgacggcgcg actccacgag tgtacgactc tgacgcgtcc agggggcaag
      121 gcgcggaggg gtggcagcgc ggccggggag acgccccgtg atagtgatgc cgtggtgatc
      181 ggcgagcggt ttgcgcatct cgtgcccgct gcggcagatc tgaccgaccc cgcgtaccag
      241 acggccgtgc gcacgctcgc cgacgagttg gcggacaccc tcggctccgc cgcagccgcg
      301 cgcgagctgg agtcggagct cgtggcggct gtggagcctg tgccgattcc gttgcgagtc
      361 gcgctcaaac tcgccatgag ccggctcacg ctggccgcgc tgacgcagcc ggtacggctt
      421 tcagtcgtat tcgccgtcta caaggagcat ctgcggatcc tccgcagcga ggagaacgag
      481 cacggcgagg acttcctgcg ccagaagctg gcgcagctcg actggctctg cgccgacacg
      541 cggcacgcct gggatctcac tgtggtcgac gacgggtgcc ccgaggggag cggcgagatc
      601 gctcgacgca tcgtcgagga gctcgatgtc gggaaccgcg tcacggtgct cttccttgcg
      661 gacgcgatcc gcgacggcct tcccgccgcg ggtggcctga cctcgaccga cgggagccgc
      721 aagggcggct cgattcgctt cggcctctgg cacgcgctgc agactcccca tccgacgcac
      781 gtccttctct acacggacgc ggacctctcg accgacctcg cccaggccgg actgctcgtc
      841 gagccgctcg tcgcagacgg ccgcacggcc gcgagcggat ctcgacgccg gcccgagtct
      901 gtcgtcgtga agggagcggg gcgcaacgtg cggggaaagc tcttcatcta cctctggaag
      961 cggctcatcc ctcagctccg gggcgtcgtc gacacgcagt gtggcttcaa ggcattcggg
     1021 gcagatggcc tgcgacggtg gctgttggag acctccgaga acggcttcgc cttcgacatc
     1081 gaatacctgc ttcgcgtcga gctcgattct ccgggatcgc tggcacgggt gccgatcgct
     1141 tggatcgaca gcgaggcgct ctcgacgacg acaggcctcc agccctaccc ggcgatgctc
     1201 cggagcgtcg tcgccctcta ccgccggtac gaggagccga caggcgacgc cgagtcgttc
     1261 gccagcctga tcgactcgct cgacgacgcg gccttcgaac ggctcctcgg tgcggttccg
     1321 cccgccattg cggatcgaga cccgcacgag ttcgacgagt tcgcagacgt cgcggccgcc
     1381 gagctcgcgg agattgcctt cgggaagacc cgataaacgc gcggcggtct gagaaaactc
     1441 aaggcggcaa gaaggccagc cgactacaat ctcgaacgat gtcgcacgcg accgacgacc
     1501 cgctcagtac ctatgccgag cctgccacgc ctcagccggc gaaggcgctc ccgagggagc
     1561 gaggtcgcca cacggtccgc ggcagaggtt cgcgagctca ccgctagcgg actttcctgg
     1621 gtcaacgccg tctcgccaga cctggatgcg gcccaggagc tggccgaccg cttcggctgg
     1681 catcccctcg acgtcgagga catcgtctcg aagcgtcagc ggcccaaggt cgacgactac
     1741 gacgaggagg gctacctctt catcgtcctc cacttcccgg tcttcgacaa ggcgatccag
     1801 cggctcaacg ccgccgagat cgacatcgtc ctcgggcacg actacctggt cacgctgccg
     1861 aaccgcgagc tgcttccggt gacgcggctc ttcgcgcgct gcgaggagga cgcggacttc
     1921 cgcgaggctc tcttcgagcg cggttcgggt cgtctcttct acgaggtgct cgacgatctg
     1981 ttcgactact gcttcccgat cctcgacaag atcgcgcaca agctcgaccg gatcgaggac
     2041 gacgtcttcg agggacgctc cgacgacgtc gtgcgcgaca tc
//
LOCUS       DBNGNK010000237         2447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1331723,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000237 DBNGNK010000000
VERSION     DBNGNK010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1331723"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="AC150V_06990"
     CDS             <1..400
                     /locus_tag="AC150V_06990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3762705.1"
                     /translation="ALSSASGTAPFDAALRRYVDFLTVDGELDVELEIDPEVRLDPDE
                     QIEIFRIVQEGLANARKHAGAHTAEVVIGQRAGRRVVVVRDDGTGFSDDEPAGQGLRN
                     MRLRAASIEGGFSLRSQQGVGTAIEVILRA"
     gene            426..1187
                     /locus_tag="AC150V_06995"
     CDS             426..1187
                     /locus_tag="AC150V_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762706.1"
                     /translation="MSAAVSQAPPPERWSRRRRMRRLGLGLFFGSVVVNAGLGIAALL
                     SGDFGETEGKVLTTSLCVTGGILLVLLCEPAWERSLLRFVPQIGSVAGVAGFGLLIAA
                     IWIEPDNAGFLPRMIGTLLTVSTGAALASLLALATLPLRLRLAFRVALALIAIAVAML
                     LPVYWADWANDWYLRVIGVVVIALAAFTVTIPVLHRVTRSAPDGSAAPIAPTPVTHCP
                     FCGGGLVASLGVETSCPACERRFVVATGARSSAET"
     gene            1200..1493
                     /locus_tag="AC150V_07000"
     CDS             1200..1493
                     /locus_tag="AC150V_07000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR3762707.1"
                     /translation="MIVRVFRARVPSERQAEFERDLRAKSLPLLDAQDGLVARFAARP
                     LESCSDVYSIVSVWRDAAALVAFAGEAWRQPVIPPGMEEQMLEAWVDHYETVA"
     gene            complement(1567..1885)
                     /gene="rnpB"
                     /locus_tag="AC150V_07005"
     ncRNA           complement(1567..1885)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC150V_07005"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1955..2161)
                     /locus_tag="AC150V_07010"
     CDS             complement(1955..2161)
                     /locus_tag="AC150V_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762708.1"
                     /translation="MTVEYLNRTLDQELAALARGVSLECLVCGEFVLHAKRGVIACAE
                     CRSLLAEGTEVSVVNTPVATGLSP"
     gene            complement(2158..>2447)
                     /locus_tag="AC150V_07015"
     CDS             complement(2158..>2447)
                     /locus_tag="AC150V_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HIR3762709.1"
                     /translation="GSADFVLRVKGESMVDAGILDGDLLVVRSQQDARNGEIVVALAG
                     DDEAADEATVKTFYREEGRIRLQPENDALEPMYPDHVQVLGKVVGVFRSLG"
BASE COUNT          311 a          845 c          846 g          445 t
ORIGIN      
        1 ggcgctctcc tcggcaagcg ggacggcgcc gttcgacgcg gcgctgcgcc ggtatgtcga
       61 cttcctgacg gtcgacggcg agctcgacgt cgagctcgag atcgatcccg aggtgcgcct
      121 cgaccccgac gagcagatcg agatcttccg gatcgtccag gaggggcttg cgaacgcgcg
      181 caagcacgca ggcgcgcata cggccgaggt cgtgatcggc cagcgggcgg gccggcgagt
      241 ggtggtcgtg cgggacgacg gcacgggctt ctccgacgac gagcccgccg ggcagggtct
      301 tcgcaacatg cggcttcgcg ctgcctcgat cgagggaggc ttctcgctgc ggtcgcagca
      361 gggagtcgga acggcgatcg aggtcatcct gcgcgcctga ccgccccggc cgggagaatc
      421 cggtcatgag tgctgccgtg tcgcaggcgc ctcctccgga gcgatggtcg cgcaggcgcc
      481 gcatgcgtcg tctcggcctc gggctgttct tcggctcggt cgtcgtcaat gcgggcctcg
      541 gaatcgccgc gctcctgtca ggcgacttcg gcgagacaga ggggaaggtt ctcacgacct
      601 cgctctgcgt cacgggtggg atcctgctcg tgctgctgtg cgaaccggcc tgggagcgga
      661 gtctgctccg gttcgtgccg cagatcggct cagttgcagg cgtcgcgggt ttcggcctcc
      721 ttatcgcagc gatctggatc gagcccgaca acgcgggctt cttgccccgc atgatcggga
      781 cgctgctgac tgtgtctacc ggcgccgcgc tcgcgtcact attggcgctg gcgactctcc
      841 cgctacgtct ccgcttggcg ttccgcgtgg ccttggccct gatcgcgatt gcggtcgcga
      901 tgcttctccc cgtctactgg gcggactggg cgaacgactg gtacctccgg gtgatcggcg
      961 tcgtggtgat cgcgctcgcg gccttcacgg tcacgatccc cgttctccac cgcgtgactc
     1021 ggagcgcccc ggacggcagc gccgcaccga ttgccccgac accggtgacg cactgtccct
     1081 tctgtggcgg cggcctggtc gcctcgctcg gagtcgagac atcgtgcccc gcgtgtgaac
     1141 ggcgattcgt cgtggcgacc ggcgctcggt cctccgctga gacctgagag gatcccggcg
     1201 tgatcgtccg cgtgtttcgc gcgcgcgtcc cctccgagcg acaagcggag ttcgagcgtg
     1261 acctgcgcgc caagtcgctg ccgctgctcg acgcccagga cgggctggtc gcacgcttcg
     1321 cggcccgccc gctggaaagc tgttccgacg tctactcgat cgtgtcggtc tggcgcgacg
     1381 cagctgcgct cgtggccttc gccggcgagg cctggcggca gccggtaatc ccgccgggta
     1441 tggaggagca gatgctcgag gcctgggtcg accactacga gacggtcgcc tgacgaagag
     1501 tcgcggctgg cccctcgcag gtgatccgat tgccgctcat gacgaagccc ccttgcgggg
     1561 gcttcgtggt agcaggcctg taagccggat tctgtcttgg atggccatct ctctgggcgg
     1621 cgcacccggt ccctcggcgg gccgcgtcgt cgggacctgc ttcgccttgc accggacggg
     1681 gtttggcgag ccgccgtgtc gccacggtcg ctggtgggct cttactccac cgtttcaccc
     1741 ttgccggcgg ttgcccgccg gcggtctgct ttctgtgcca ctttccgtcg gctttcgccg
     1801 cctgggattg ccccagcgtc ctgccctgcg gtgtccggac tttcctcgag cgccggcggg
     1861 cgctcgcggc cacccggcct gcactctgaa cggtagcgcc gtcccgtggc cgatgccagc
     1921 ggcgcgaccg ccgcatggag agcgccgcga acctctacgg ggacagtcct gtcgccacag
     1981 gagtgttgac gacggaaacc tccgtgccct ctgcgaggag tgagcgacat tcggcgcagg
     2041 cgatcacgcc gcgcttggcg tgcagcacga actcgccgca cacgaggcac tcgagactga
     2101 ccccgcgcgc cagggcggca agctcctggt cgagcgtgcg gttcaggtat tcgaccgtca
     2161 ccccaggctc ctgaacacgc cgaccacctt cccgagcacc tgcacgtggt cggggtacat
     2221 aggctcgaga gcgtcgttct cgggctgcag cctgatgcgg ccttcctccc gatagaaggt
     2281 cttcacggtg gcttcgtcgg ctgcctcgtc gtcgccggcg agcgcgacga cgatctcgcc
     2341 gttccgagcg tcctgttggc tccgtacgac gagcaggtcg ccgtcgagga tgcccgcgtc
     2401 gaccatcgat tcgcccttga cgcgaagtac gaagtcggcg gagccga
//
LOCUS       DBNGNK010000238         3271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1547996,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000238 DBNGNK010000000
VERSION     DBNGNK010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1547996"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC150V_07020"
     CDS             <1..554
                     /locus_tag="AC150V_07020"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3762710.1"
                     /translation="PAGATTYLCAVDGDGVAVSLIQSIFGSFGSGVVAPRTGVVLQNR
                     AGGFVEQEGHPNRLGPAKRPFHTIIPGMLLEGDHLLGPFGVMGGPMQHQGHFQVVHHL
                     VDEGADPQASLDHPRWRVGDDWSVDLEPGLWGEAERLRRLGHDVGLAECQHPFGVGQA
                     ILRFGESLIAGSDGRGDGYAAGF"
     gene            complement(565..1209)
                     /locus_tag="AC150V_07025"
     CDS             complement(565..1209)
                     /locus_tag="AC150V_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3762711.1"
                     /translation="MKVKEIMISDVQTVAPETPLKDAARLLAINHFSGVPVVDSDDYV
                     LGVLSEADILVKEGRPEEHHGVLHWLLDPDDPTLAEKLNAKTAGEAMTSPAITIAPGR
                     PLRVAANTMLEQGVNRLPVVGDDGRLIGLVSRGDLVRAFVRSDAAVKQEIEEDVLRRI
                     LWVDDPYAVTVSVSAGTATLEGKVANEEDLKLLPRFVERVPGVVAVESKLTVAR"
     gene            complement(1678..1950)
                     /locus_tag="AC150V_07030"
     CDS             complement(1678..1950)
                     /locus_tag="AC150V_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762712.1"
                     /translation="MEDAVALLEHPVSSPLELAEWSPSELRRVVVRLTDGEVVQVGTA
                     PNRDGALALARTVMAEIDEPGGEWPLVGDRLLRPDSIVSVDVLATG"
     gene            complement(2027..2398)
                     /locus_tag="AC150V_07035"
     CDS             complement(2027..2398)
                     /locus_tag="AC150V_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762713.1"
                     /translation="MTVYPGDHDLEENLRPVGHEADWATDESLETLLQPHPPAFRSDL
                     RRVVVRMQGGEEIEVGKAIGREAAVVIARELVAAIESSTDSCEWPEFGDRFLRPGAIV
                     SIDVQRAAPADSPDPSADSLD"
     gene            complement(2447..>3271)
                     /locus_tag="AC150V_07040"
     CDS             complement(2447..>3271)
                     /locus_tag="AC150V_07040"
                     /inference="COORDINATES: protein motif:HMM:NF020694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HIR3762714.1"
                     /translation="GSPARMTTLGLLFPGEMGAVLGRCARADVVVWSSAERSPATTDR
                     AEAAGLVDAGGLEDLVRASDIVLSVCPPAIAEDVARDVAALGFDGLYVDANAVSPERM
                     VRIAALHPGAVDGSIIAKSALRLYLSGAPADVAAVAALYAETEVEAVALDGPIGAASA
                     LKAAFAGWNKITVALEAQAHSLAKAWGVSPELRAEGVDTARVERVAGRAWRWAPEMEE
                     IGDALAEQGIPSGIAVGAAALFARWSGHRDEQVLLETLLAELRDDDPRRAPPTSDP"
BASE COUNT          470 a         1138 c         1150 g          513 t
ORIGIN      
        1 tccctgccgg cgcgacgacg tacctctgtg ccgtggacgg tgacggcgtc gccgtctccc
       61 tgatccagag catcttcggc tcgttcggct cgggcgtcgt agcgccgcgg acgggcgtgg
      121 ttctccagaa ccgggcaggg ggcttcgtcg agcaagaagg gcatccgaac cgtctgggtc
      181 ccgcgaagcg cccgttccac acgatcattc cgggcatgct gttggagggc gaccatctgc
      241 tcgggccgtt cggggtgatg ggaggcccga tgcagcatca ggggcacttc caggtggtgc
      301 atcacctcgt cgacgaggga gcagatccgc aggcctcgct ggaccacccc aggtggcggg
      361 tcggggacga ctggagcgtc gacctcgagc cggggctgtg gggcgaggcg gagcgcctgc
      421 gccggctcgg ccacgatgtg ggactggcgg agtgccagca tcccttcgga gtcgggcagg
      481 cgatccttcg cttcggcgag tcgctgatcg ccgggtcgga cggccgcggc gacggttacg
      541 cggccggctt ctgagcgcgc ggatctaccg cgcgaccgtc agcttcgact cgaccgcgac
      601 gacgcccggg acgcgctcga cgaagcgggg cagaagcttg aggtcttcct cgttcgcgac
      661 tttcccctcg agcgttgcgg tgcccgcgct gacgctcacg gtgaccgcgt acggatcgtc
      721 gacccagagg atccggcgaa ggacgtcttc ctcgatctcc tgcttgaccg ccgcgtcgct
      781 gcggacgaac gcgcgcacga ggtcgcctcg cgatacgagc ccgatgagcc tcccgtcgtc
      841 gccgacgacg ggcaggcggt tcacgccctg ttccagcatc gtgttcgccg ctacgcgcaa
      901 cgggcgcccc ggtgcgatcg tgatagccgg cgacgtcatc gcctctccgg cggtcttcgc
      961 gttgagcttc tccgcgagcg tagggtcgtc ggggtcgagc agccagtgca gcaccccgtg
     1021 gtgctcctcg gggcggccct ccttcacgag gatgtccgcc tcggagagga cgccgagcac
     1081 gtagtcgtcc gagtcgacaa cgggaacgcc cgagaagtga ttgatcgcta gcagccgggc
     1141 ggcgtccttg agtggggtct ccggggcgac ggtctggacg tcgctgatca tgatctcctt
     1201 gaccttcatc gggctcctcc gtggggtagt gaccggaaag ataatcgcct gcagttccgc
     1261 tagctcaagt gtccctcgcg gcgcaggtgt ccgcatccgc gacgggcgcg gagggctcgc
     1321 gggaaacccg cagcgaaccc gggtgagcga cgatcacgca gtgcaggagc ggcttcgagt
     1381 cgggctgttc acgacgatcc tcccggctgc gatcggaatc gacgccatcg tgcggcacgc
     1441 gaatcacgag ccggccgccg tcttcgttcc caggcggggc cctccctcga gctcgacggc
     1501 cagcgcttgc gccctctccg ggcgaccccg acgagccgga cgagacggcg ctacgcctcg
     1561 atgcgcgcga cgggccggcc tgggtactcg agcacgagtc ggttgagccc tgatgaaggc
     1621 tcgggcagtg gacggccctc ggcgtggccg ctcggggtca cgcgtgcggt gcgaccgcta
     1681 gccggtcgcc agaacgtcga ctgagacgat cgagtcgggg cgcagcagac ggtcgcccac
     1741 gagcggccat tcgccgcccg gttcgtcgat ctcggccatc acagtccgtg cgagcgcaag
     1801 agcgccgtcc cggttcgggg cggtgccgac ttgcacgact tcgccgtcgg tgagccggac
     1861 cacgacacgg cgaagctccg acggtgacca ttcggccagc tccagtggag agctgacggg
     1921 gtgttcgagc agtgccacgg cgtcctccat catgttgagt ctgggagtcg ccgcatactc
     1981 cctgcctgga tcggttatcg gcgcgcgggg ccgaaggctg tgtccctcag tcgagcgaat
     2041 ctgccgacgg gtcaggcgag tcggcgggcg ctgctcgttg gacgtcgatc gagacgatcg
     2101 cgcccggacg caggaagcga tcgccgaact cgggccactc gcagctgtcc gtcgaggact
     2161 cgatcgccgc aacgagctcg cgggcgatca cgaccgcagc ttcacgtcca atcgctttcc
     2221 caacctcgat ctcttcgccg ccctgcatgc ggacgacgac ccgccggagg tctgagcgga
     2281 atgcgggagg gtgcggctgg agcagcgtct cgagcgactc gtctgtcgcc cagtccgctt
     2341 cgtgccccac gggccgcaga ttctcctcga gatcgtggtc gcctggatac acggtcaccc
     2401 gtccggtatc ggtcgatcga agggtgcttc agacccctcg atcgagtcac ggatccgagg
     2461 tgggcggcgc gcggcgcggg tcgtcgtcgc gaagctcggc gaggagggtc tccagtagta
     2521 cctgctcgtc gcggtggccc gaccagcgcg cgaacagcgc ggcggcgccg acggcgattc
     2581 cagaagggat tccctgctcg gcgagcgcgt cgccgatctc ctccatctcg ggcgcccaac
     2641 gccaggctcg gccggcgacc cgctcgacgc gggccgtgtc gacgccctcg gcccgaagct
     2701 cgggcgatac gccccacgcc ttcgccaggg agtgtgcctg cgcctcgaga gcgacggtga
     2761 tcttgttcca gccggcgaag gcagccttga gtgccgaggc ggcgccgatc ggcccgtcga
     2821 gcgccacggc ttccacctcg gtctctgcgt agagcgctgc caccgccgcg acgtccgccg
     2881 gcgcgcccga gaggtagagg cgcagcgccg acttggcgat gatcgacccg tccaccgcgc
     2941 ccgggtggag cgccgcgatg cggaccatcc gctcgggcga gaccgcgttg gcgtcgacgt
     3001 agaggccatc gaatcccagc gccgccacgt cgcgtgccac gtcctcggcg atcgccggcg
     3061 ggcaaacgct cagaacgatg tcgcttgccc gcacgagatc ctcgagcccg cccgcgtcga
     3121 ccagccccgc cgcctccgcg cgatccgtcg tggccgggct ccgctcggcc gaagaccaca
     3181 cgacgacgtc cgcgcgcgcg cagcggccga gcacggcgcc catctctcct gggaagagca
     3241 gcccgagggt cgtcatcctt gctgggcttc c
//
LOCUS       DBNGNK010000239        10129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1620618,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000239 DBNGNK010000000
VERSION     DBNGNK010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10129
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1620618"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="AC150V_07045"
     CDS             complement(<1..531)
                     /locus_tag="AC150V_07045"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR3762715.1"
                     /translation="MATTTARATTDLLRPFVPRLAADWLGETPSERHRAYAGTLVFAD
                     VSGFTELTEKLSAKGRAGSEEIADVLDAAFAELVAAAYSYDADLLKWGGDAILLLFRG
                     DDHTVRAAAACSAMQRALVEMRRLRTSFGPVRLQMSIGAHAGLFHCFLVGSVHRELVV
                     TGSDATITVRTEATADA"
     gene            complement(565..1644)
                     /locus_tag="AC150V_07050"
     CDS             complement(565..1644)
                     /locus_tag="AC150V_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR3762716.1"
                     /translation="MNTAVDLKPPADGARSCFPDAHFDALLVVSFGGPEGMDDVLPFL
                     ENVTSGRGVPPERLEEVAEHYARFDGVSPINAQNRALIAALRDELSAHGIELPIYFGN
                     RNSHPFLTEALREMADAGVERALAFFTSGFSSYSSCRQYRENIYDAQQAAGAAAPEIA
                     KTRMFYNHPGWIEANAELLAGALADQPEAHVLFSAHSIPAAMAEGCRYEAQLAESARL
                     VAEAAGANRWQVVYQSRSGPPHVPWLEPDVCDTIEELAANGADAVVVSPLGFVSDHLE
                     VLYDLDVEARETAAAGGMAFTRVPSVGTHPTFVAMIRELIEERLDPSVARRTEGQGGP
                     SHDVCPPNCCPPGTGRLSPWAEADV"
     gene            1770..1982
                     /locus_tag="AC150V_07055"
     CDS             1770..1982
                     /locus_tag="AC150V_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008416943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762717.1"
                     /translation="MSTITPEAPTTWPQLAEGLYSFLTDRGATIEYEFDQMNVRVPRD
                     AGDDAPQARWRIDGTLRIRTAESRRA"
     gene            1979..2299
                     /locus_tag="AC150V_07060"
     CDS             1979..2299
                     /locus_tag="AC150V_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762718.1"
                     /translation="MIAVDGTLALEIDDAPVQVVAEGDRLRVDAERPSTLLRSGSPGA
                     LRKLAAAADYLGLTVELTAGGRTLLVAGRGARRGIAGRLLRLDHVAVPLRSLPLLLRS
                     RFGG"
     gene            2342..2638
                     /locus_tag="AC150V_07065"
     CDS             2342..2638
                     /locus_tag="AC150V_07065"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3762719.1"
                     /translation="MTRLLDPEGEHLAALRRLGDFSGARVCEVGCGDGRLTPGIASEA
                     RYVFAFDPDAEAIAEARRRLPKELHDVVTYEAGSATEVEIPRAEFDIVVFSWSL"
     gene            2642..3037
                     /locus_tag="AC150V_07070"
     CDS             2642..3037
                     /locus_tag="AC150V_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762720.1"
                     /translation="MEREDVVRVLRRFRTALAAPGGVMLDLQVVPPRPVVEVDGAVVC
                     SVEGGVLLDGAAAATAAVDGMIARGLLREDGFDDHEVLHHYASGAELLADFETKRRSI
                     PTEAAPALAAVERPCLIREACRARRLLVA"
     gene            complement(3191..3796)
                     /locus_tag="AC150V_07075"
     CDS             complement(3191..3796)
                     /locus_tag="AC150V_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762721.1"
                     /translation="MLALALPAAALADEGGRKSEKAPTAESQTEGQQPTASQTCKRQR
                     TDLGIAAFKLLYGSNRNKSNALGKCVAKIATAKRVAAANAAKTCKAERAMTDDEFRAG
                     HDGKTFDEVYGSGKTGRNAFGKCVSQHAKDNADDAVEAVTNAAKTCKAEKRMAAAAFA
                     AAHGGKSFAEHYGTNMRKTNAFGKCVAAAVKQAASTQPQTN"
     gene            4245..4973
                     /locus_tag="AC150V_07080"
     CDS             4245..4973
                     /locus_tag="AC150V_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762722.1"
                     /translation="MDTDRHSAHGIALEHIPEPELGLQVDVAGRRLLVAVVAVVAAVP
                     IVAATGLAMPLPSSLLKAAASLVPASSSETVELSSSNTPGAPGAVAITPTVQEKERLR
                     AASSVHASAPPERAAVAASAVPAASAAKPRAKTSGGPHKRERSGPKPITIQVNRGNPT
                     ATTVVRASAPSTPAVSKAKRVPAPVQARTTPVVIAQPPTAVEPPKPPKAVEPPEELAL
                     QPHAPAPPSNSNAGSGGHGNPNKG"
     gene            4975..6957
                     /locus_tag="AC150V_07085"
     CDS             4975..6957
                     /locus_tag="AC150V_07085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3762723.1"
                     /translation="MAGEPAPSGAIPRRQPRLVLRFAIVTALGLAAAGALILLVVRHV
                     DEQQAQRASTDRARLVAETHLRDRLEPADARAPVKGLRRTELDRFVRRTLRLDGLLRL
                     SVVGHGGRVTYSTDHRLIGRPAADPALIAEARAGSLVSSVATVPSVQGSGELKALLVR
                     VPVALGKGPPAVVVFEQDYGPIATDARESFLPIAGVLEVALVALFALLVPTLARSSRR
                     LRDYVAEIRYRATHDTLTGLPNRMQLYQSIADALAVRADYGSLAVLLVDLDRFKEVND
                     SLGHDAGDELLCEIADRLVTAAPDALVARLGGDEFGVLLCDAKDADAFAAAAGLRERI
                     AEAFSVRGIPVWVDASIGVALAPTHGTETTQLLRRADVAMYVAKRGRLGVALYDPESD
                     LNDTGRLVLMTELRTALERDELDVFYQPIVHLETGEIASVEALVRWNHPARGLLAPSA
                     FLPLAEHTGLILEVNRLVARRAVQQCADWHRLGVDLSVAVNFTVLDLLDPKFVGEMRR
                     LMAENDLAPAFLTVEVTEDVLMQEPERIGRTLADLRAVGIRVAIDDFGTGYSSLAYLR
                     QLPVDGLKIDRSFVNEVPHQAASVAIIRSVVELAKQLGLDVVAEGVEEASQADLLEEA
                     GCDLIQGYLVSEPVAAHEITGWIESPAPTQAATTVA"
     gene            complement(6983..8575)
                     /locus_tag="AC150V_07090"
     CDS             complement(6983..8575)
                     /locus_tag="AC150V_07090"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphohydrolase"
                     /protein_id="HIR3762724.1"
                     /translation="MNVTQSGPGRFSALGCLAFSRASAAARTVFFGRRRHAEGGEQES
                     SEAILRIVSSIDDYLYTNEHRADGSRRSIFSGPNREKLMGGPLPPGADAASEWERLIH
                     PDDFDEHLAHRARLRRGEPSEVCYRLIGYDGVTRWVKARTKPHHVDGRVFVEGIVSDV
                     TRQLLAEQELAGAQDELRRLAAVNEHQALHDALTGLGNRRKLLQDLEHTIMEASESAP
                     CLLVLFDLDGFKQYNDTFGHPAGDALLALLGAKLQAALGGRAASYRLGGDEFCVLLPT
                     ADSDVETSISILSRALTDEGESFTITSSFGAVFLPAQAKSSEEALHLADQRLYNAKRS
                     RSSRRSRPHDVLLQVLLEREPGLHGHTAEVAELARAMAVLLDLDEVAIHTLDQAALMH
                     DIGKLAVPDAILQKPGPLDDSEREVIKRHTIVGERILSASPMLTGVAKIVRATHERHD
                     GSGYPDGLAADEIPLEARVVSVCNAYSALTSDRPYRRARSHEEAVAEIRRCSGTQFDP
                     VVVESFLSAVETQHSQAGSSAA"
     gene            8741..9925
                     /locus_tag="AC150V_07095"
     CDS             8741..9925
                     /locus_tag="AC150V_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762725.1"
                     /translation="MQRSIYCYAWDLADDGVEAAVRRFDECGLNTATFAGSYHAGKFL
                     RPHGRDGKVYFPEDGTIYFRSSGSRFGEIEPVANSLLQDRDVLRELCDDGTLAVNAWL
                     VLLHNSRLGQTHLDATVANAFGDRYIYSLCPSAPAAREYAVALCASITDEYPVSGLRL
                     ETPGFLPFAHGYHHEFGLVRQNDWLERLLGLCFCRHCADGVRDAGIELDGLRGRVASA
                     IETYLASDVDYPDDMAEAFWLADVVLDSDLGAFLRWRCDVVTSLVEAIRAEVPMNVDV
                     AIIPSVARPSGGAWYEGSDLAALARAAGGVEVCFYEHGADRVAADLLDVQRRIGDAGR
                     ISAVLRPGHPDLSDRTMVAAAVATLARGGIHDVGFYNYGHLRASALDWIAEALSATEE
                     RA"
     gene            9922..>10129
                     /locus_tag="AC150V_07100"
     CDS             9922..>10129
                     /locus_tag="AC150V_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762726.1"
                     /translation="MSDFTGRVVLITGAAGGIGQALSRLFGERGAAIAALDRDDAVND
                     FAAALHDEGIESAAAVADVGAADEI"
BASE COUNT         1486 a         3442 c         3481 g         1720 t
ORIGIN      
        1 cgcgtcggcc gtggcctcag tccgcaccgt tatcgtcgcg tcgctcccgg tcaccacgag
       61 ctcgcggtgc acggagccga cgaggaagca gtggaagagc cctgcgtgcg cgccgatcga
      121 catctgcagc cgaaccggac cgaacgacgt ccgcagcctc cgcatctcga ccagagcgcg
      181 ctgcatggcc gagcaggccg ccgcggcccg caccgtgtga tcatcaccgc ggaagagcag
      241 cagaatcgcg tcgccgcccc acttgagcag gtcggcgtcg tacgagtacg cggctgcgac
      301 gagctccgcg aaggcggcgt cgagaacgtc tgcgatctcc tccgagccgg cccttccctt
      361 cgccgagagc ttctcggtca gctcggtgaa gccggagacg tctgcgaaca cgagcgttcc
      421 cgcataggcg cgatggcgct cgctcggcgt ctcgccgagc cagtcggcgg cgagcctcgg
      481 cacgaagggg cggaggagat cggtcgttgc ccgagcggtc gttgtcgcca cgtcgcgtcg
      541 agcgtaccgc ggcggtcgct gtgtctagac gtctgcctca gcccacgggc tgagccgccc
      601 cgtcccgggc gggcagcagt tcggcgggca cacgtcgtgg ctcgggccgc cctggccctc
      661 tgtccgccgg gcgacggacg gatcgaggcg ctcctcgatc aactcacgga tcatcgcaac
      721 gaaggtgggg tgggtgccca cgctcggcac gcgggtgaag gccattccgc cggccgctgc
      781 tgtttcgcgg gcctcgacgt cgaggtcgta gagcacctcg agatgatcgg agacgaagcc
      841 gagcggcgac acgacgaccg cgtctgcccc gttcgccgca agctcctcga tcgtgtcgca
      901 cacgtccggc tcgagccacg gtacgtgcgg tgggccgctg cgggactggt acaccacttg
      961 ccagcggttc gcaccggcgg cctcggcgac gagccgtgca gactccgcca gctgcgcctc
     1021 gtaccggcag ccctccgcca tcgcggccgg gatcgagtgg gccgagaaca ggacgtgcgc
     1081 ctccggctga tcggccagcg ctcccgcgag cagctcggcg ttggcctcga tccaccccgg
     1141 gtggttgtag aacatccgcg tcttcgcgat ctccggagcg gcggcaccgg cggcctgctg
     1201 tgcgtcgtag atgttctcgc ggtactgccg gcagctcgag tacgagctga agccggaggt
     1261 gaagaacgcc agcgcccgct cgacaccggc gtccgccatc tcgcgcaggg cttcggtgag
     1321 gaacgggtgc gagttgcggt tgccgaagta gatggggagc tcgatgccgt gcgccgacaa
     1381 ttcgtcgcgg agtgccgcga tcagggcgcg attctgggcg ttgatcgggc tgaccccgtc
     1441 gaagcgtgcg tagtgctctg cgacttcctc gagccgctcc ggaggaaccc ctcggccgct
     1501 ggtgacgttc tcgaggaacg gcaggacgtc gtccatccct tcaggaccgc cgaatgacac
     1561 gacgagaagg gcgtcgaagt gtgcgtcggg aaagcagctc cgggcgccgt cagcaggtgg
     1621 tttgaggtca acggcggtgt tcattccagc gcgcgcaacg tagcagtccg gccgcgacca
     1681 cgggcggtgg tagaaactag acaaatactt gacaagaatc ggacgatggc ctattatagc
     1741 cgcattgcat caatcccgaa ggaggaacaa tgtccactat cacccccgaa gcgccgacca
     1801 cgtggcccca gctcgccgag ggtctctact cgttcctgac cgaccgcggc gccaccatcg
     1861 agtacgagtt cgatcagatg aacgtgcgcg tcccccgcga cgccggtgac gacgcgcctc
     1921 aggcccgctg gcgcatcgat ggcaccttgc ggatccgcac ggcggagagc cgccgcgcgt
     1981 gatcgccgtc gacggcacgc tggcgctcga gatcgacgac gcgccggtac aggtcgttgc
     2041 ggagggcgac cggcttcgag tcgacgcgga gcgtccgtcg acgctcctgc gctcggggtc
     2101 gccgggcgcc ctccgcaaac tcgccgctgc ggccgattac ctcggcctca ccgtcgagtt
     2161 gaccgcgggc ggccgaacgc ttctcgtcgc gggacgcggc gcccgtcgcg gcatcgcggg
     2221 aaggctcttg cgtctcgacc acgttgcagt tccgctccgg tcgctcccgc tgctgctccg
     2281 ctcgcgcttc ggcggatagt cgcgcccagc gacctgccgc ccgaggcgcg ataatcgcca
     2341 cgtgacccgg ctgctcgacc ctgaagggga gcacctggcc gcgctgcgcc ggctgggcga
     2401 cttctcgggc gctcgggtct gcgaggtggg ttgcggcgat gggcggctca cgccgggaat
     2461 tgcctccgag gcgcggtatg tgtttgcatt cgatcccgac gccgaggcga tcgcggaggc
     2521 gaggcgccgt ctgccaaagg agctccacga cgtcgtcacg tacgaggcgg gctcggcgac
     2581 cgaggtcgag atcccccggg ccgagttcga catcgtggtc ttctcttggt cgctctgatg
     2641 aatggagcgg gaggacgtcg tgcgcgtcct gcgtcgtttc cggactgcgc tcgccgcgcc
     2701 cggcggagtg atgctcgacc tccaggtcgt ccccccacgg ccggtggtcg aggtcgacgg
     2761 cgcggtcgtc tgctccgtcg agggtggcgt gctgctcgac ggagcggctg cggctactgc
     2821 cgctgtcgac ggcatgatcg cccggggcct gctccgcgag gacggcttcg acgaccacga
     2881 ggtgctgcac cactacgcgt ctggtgccga gctcctggcg gacttcgaga cgaagcggcg
     2941 ctcgatcccc accgaggcgg cccccgctct cgctgcggtg gagcgaccgt gtctgattcg
     3001 tgaggcctgt cgagcgcgcc ggcttctagt tgcctgacgc tggcggcggt gcgttgacct
     3061 acgaactggt tgaaggccga tccggcagca tctgcgcagg gcgcacggtt ccggcacgcc
     3121 gaaaggggcg gagcgccgcc gcattggcgc tccgccccgg gcgggggacg caggagggga
     3181 aagcgtctat ctagttcgtc tggggctggg tgctcgccgc ctgtttcacg gctgcggcga
     3241 cgcacttgcc gaaggcgttc gtcttgcgca tgttggtgcc gtaatgctcg gcgaacgact
     3301 tgccgccgtg ggctgcggca aaggccgcgg cagccatccg cttctcggcc ttgcaggtct
     3361 tcgcggcgtt ggtgaccgct tcgacagcgt cgtcggcgtt gtccttcgcg tgctgcgaaa
     3421 cgcacttgcc aaaggcgttc ctaccagtct tgccgctgcc gtagacctcg tcgaacgtct
     3481 tgccgtcgtg gccggccctg aactcgtcgt cggtcatcgc ccgctccgcc ttgcaggtct
     3541 tcgcggcgtt ggcggctgcg acccgcttcg ccgtcgcaat cttcgcgacg cacttgccga
     3601 gcgcgttgga cttgttcctg ttcgacccgt acagcaactt gaaggcggca atgcccaagt
     3661 cggtgcgctg ccgtttacag gtctggctgg cggtgggctg ttggccctcg gtctggcttt
     3721 cggccgtcgg cgccttttcg ctcttgcgcc cgccctcgtc ggcgagtgcc gcagccggaa
     3781 gcgcgagggc gagcaacgcc gcgagtgtga tcagcttccg catgtccctt cgctccttct
     3841 cgtggggtta cttgcgtgct ggtctcatcg tctgcatggg catcgggctc gcgcccccag
     3901 agagggtgag gaccggtaag gacttcggcg tgatttcgta aacggcatgt gatgccggcg
     3961 cacgacgacg cgctccacgg gtgtcggtgc tgtcacgtcg agttgctccg ccgccccgtc
     4021 cgtgacggcg tagacgctgt cctcccgcgg cttggtcatc tgctccggcc gtttcgtcgc
     4081 gctctgattc ccgatacgaa gtggtcacag acgacgcgac gatggccgcg gacgctgcga
     4141 agcgactccg ggtctccctg gttcgaggta ccccgccaca cctctacccc tgcggtaacc
     4201 cgatcagggg atgctcaccc atccgatggg actaccttga ggccgtggac accgatcgtc
     4261 actccgcaca cggaattgcc ctcgagcaca tcccggagcc ggaactcggc ctccaggtcg
     4321 acgtggcggg acgtcggctg ctcgtcgcgg tcgtcgcggt cgtcgcggct gtgccgatcg
     4381 tcgccgcgac cggcctggcg atgccgttgc cgtcctcgct gctgaaagcg gcggcgtcgc
     4441 tcgtccccgc ctcgtcgagc gagactgtcg agctcagctc gagcaacact cctggtgcgc
     4501 ccggcgcggt cgccatcacg ccgaccgtgc aagaaaaaga gcggctgagg gctgccagct
     4561 cggttcacgc atccgctcca ccggagcgcg ctgccgtcgc ggcctcggcc gttccagcgg
     4621 cgtcggccgc caagccgcga gccaagacgt cgggcggccc tcataagcgg gaacgctcag
     4681 ggcccaagcc catcacgatc caggtcaacc ggggtaatcc gaccgctacg acggttgtcc
     4741 gtgccagcgc gccgagcaca cccgctgtgt cgaaggcgaa gagggtgccc gcgccagtgc
     4801 aggcgcgtac gacgccagtc gtcatcgctc agccgccgac ggctgtcgag ccgccgaagc
     4861 cgccgaaggc tgtcgagccg ccggaagaac tggcgcttca accccacgca cccgcgcccc
     4921 cgagcaacag caatgccggg agcggcggtc acggcaaccc gaacaagggc tgacgtggca
     4981 ggcgagccag cgccgtcggg ggcgattccg cgtcggcagc cgcgcctcgt gctgcgcttc
     5041 gcgatcgtca cggctctcgg ccttgccgcc gcgggcgctc tgatccttct cgtcgttcgg
     5101 cacgtcgacg aacaacaggc ccagcgagcg tcgacggaca gggctcggct cgtggccgag
     5161 acccacttgc gggatcgcct ggagccggcc gatgcgcgcg cgcctgtcaa gggcctacgc
     5221 cgcacggagc tcgaccgatt cgtccgcaga accctccgcc tcgacgggct gttgcggttg
     5281 agtgtcgtcg gccacggagg ccgcgtgacg tactccaccg atcatcggct gatcgggcga
     5341 ccggccgccg atcctgcgct catcgcagag gcgcgggccg gctcacttgt ctccagcgtc
     5401 gccaccgtgc cgtcggtgca agggtctggc gaactcaaag ccctcctcgt gcgtgtgcct
     5461 gtcgcgctcg gcaagggccc tccggcagtc gtcgtattcg aacaggacta cgggccgatc
     5521 gcgacggacg cacgcgagtc tttcctgccg atcgcgggcg tgctcgaggt cgcgctggtt
     5581 gcattgttcg ccctgctcgt gccgaccctc gctcgctcga gtcgacggct tcgtgactac
     5641 gtcgcagaga tccgctacag agcgacgcac gacaccctga cgggcctgcc caaccggatg
     5701 cagctctatc agtccatcgc ggacgcgctg gccgttcggg ccgactacgg atctctcgct
     5761 gttctgctcg tcgatctcga ccgcttcaag gaggtcaacg actcgcttgg ccacgacgca
     5821 ggcgacgagc ttctctgcga gatcgcggac cggctcgtca ccgccgcacc ggacgcgctg
     5881 gtcgctcggc tcggtggcga cgagttcggg gtgttgctct gcgacgcgaa ggacgccgac
     5941 gcgttcgcgg ccgcagcagg gcttcgcgag cggatcgcag aggcgttctc cgttcgaggc
     6001 atcccggtct gggtcgacgc cagcatcggc gtggcgctcg ctccgacgca cggtaccgag
     6061 acgacccagc tgctgaggcg tgccgacgtg gcgatgtacg tcgcgaagcg cggccggctg
     6121 ggggtcgcgc tctacgaccc cgagagcgac ctcaacgaca ccggccggct cgtcctgatg
     6181 accgagttgc gcaccgcgct ggagcgggac gaactcgacg tcttctacca gccgatcgtt
     6241 cacctcgaaa ccggcgagat cgcgtcggtc gaggcgctgg tccgctggaa tcaccctgcg
     6301 cgaggtctgc tcgctccgtc ggcgttcctc ccgctcgccg agcatacggg tctcatcctc
     6361 gaggtcaacc ggctcgtcgc ccggcgcgcc gtacaacagt gcgcggactg gcatcggctc
     6421 ggcgtcgatc tcagcgtcgc tgtcaacttc acggtgctcg acctactcga cccgaagttc
     6481 gtaggcgaaa tgcgccggct catggcagag aacgatctcg ctcccgcctt tcttaccgtc
     6541 gaggtcactg aggacgttct gatgcaggag cccgagcgga tcggacgtac gctggccgat
     6601 ctccgagcgg tggggatccg cgtcgctatc gacgacttcg gaacgggata ctcgtcgctg
     6661 gcgtatcttc ggcagctgcc ggtcgacggg ctgaagatcg atcggtcgtt cgtcaatgag
     6721 gttccgcatc aggccgcgag tgtggcgatc atccggtcgg tcgtggagct cgccaagcag
     6781 cttgggctcg acgtcgtcgc ggagggcgtc gaagaggctt ctcaggccga cctgctcgag
     6841 gaggccggct gcgacctgat tcagggctat ctggtgtcgg agcccgtggc cgcgcacgag
     6901 atcacgggct ggatcgagtc acctgcgccc acccaggcgg ccacgaccgt cgcctgaccg
     6961 agcggcgttc gtcttgcgtc cgctatgcgg cggaggagcc cgcctggctg tgctgcgtct
     7021 ccaccgccga caggaacgac tcgacgacca ccgggtcgaa ctgggtgccg ctgcagcgcc
     7081 ggatctccgc aacggcctcc tcgtgcgacc gcgcgcggcg gtacgggcga tccgaggtga
     7141 gggccgagta cgcgttgcag acggagacga ctcgcgcttc gagcggaatc tcgtccgcgg
     7201 cgagcccgtc cgggtagccg ctgccgtcgt ggcgctcgtg cgtggcccgc acgatcttgg
     7261 cgacgccggt gagcatcggc gaggccgaga gaatgcgctc gccgacgatc gtgtgtcgct
     7321 tgatgacctc cctctcggag tcgtccaggg ggccgggctt ctgaaggatc gcgtcgggaa
     7381 ccgcgagctt gccgatgtcg tgcatgagcg cggcctggtc gagcgtgtgg atcgcgacct
     7441 cgtcgaggtc gagaaggaca gccatcgctc gcgcgagctc tgcgacctcg gcagtgtgcc
     7501 cgtgaagccc gggctcccgc tcgagcagca cttggaggag gacgtcgtgc ggtcgactgc
     7561 gccgcgagct acgagagcgt ttcgcgttgt agaggcgctg atcggcgaga tggagcgcct
     7621 cctccgagct cttcgcctgg gcgggcaaga agaccgcccc gaacgagctc gtgatcgtga
     7681 agctctcgcc ctcgtcggtg agcgccctgc tcaggatcga gatcgacgtt tcgacgtcgg
     7741 agtcggcggt cgggagcagg acgcagaact cgtcaccgcc gagccggtag ctagcggctc
     7801 gtccgccgag ggctgcctgg agcttcgcgc cgagcagggc caggagcgca tctccggccg
     7861 ggtggccgaa cgtgtcgttg tactgcttga agccgtccag gtcgaagagc acgagtaggc
     7921 aaggtgcgga ctccgatgcc tccatgatcg tgtgctccag gtcctgaagc agcttgcggc
     7981 ggttcccgag ccccgtgagt gcgtcgtgga gtgcctgatg ctcgttgact gcggcgagac
     8041 ggcgaagctc gtcctgcgcg cccgcgagct cctgttcggc caacagctgg cgggtcacgt
     8101 cggagacgat cccctcgacg aagacgcgtc cgtcgacgtg gtgcggcttg gtccgtgcct
     8161 tcacccagcg ggtaacgccg tcgtagccga tcagccgata gcacacctcg ctgggctccc
     8221 cccggcggag ccgggctcga tgcgccaggt gctcgtcgaa gtcgtccggg tgaatcaggc
     8281 gctcccactc ggacgcggcg tcggccccgg ggggcagcgg gccgcccatc agcttctccc
     8341 ggttcggccc ggagaagatc gagcgccgtg acccgtccgc acggtgctcg ttcgtgtaga
     8401 ggtagtcgtc gatcgacgag acgatgcgga gaatcgcttc gctcgactcc tgttcgccgc
     8461 cctctgcgtg gcggcgacgc ccgaagaaca cggtgcgtgc ggcggccgag gctctggaga
     8521 aagcgaggca gccgagtgct gagaagcgcc cgggaccaga ttgcgtcacg ttcatcgcta
     8581 tcggcgtgag ccgcgacagc ttgaaccgag cgacgtgaga aggtaggtcg gttcacccgt
     8641 caagctgttc cggcgccgat gtgtttaggt tttcgagcac gagcagccgc tgagaggcag
     8701 cgctcgcgaa accgaactac gtccgtgggg gtgagccacg ttgcagcgtt cgatctattg
     8761 ctacgcctgg gaccttgccg acgacggcgt cgaggccgcc gtccgccgtt tcgacgagtg
     8821 tgggctcaac acggcgacct tcgccggcag ctaccacgcg ggcaagttcc tgcgacccca
     8881 cggccgcgac ggcaaggtct actttccgga agatggcacc atctatttcc ggtcgtcagg
     8941 cagcaggttc ggtgagatcg agccggtcgc caacagcctc cttcaggatc gcgacgtcct
     9001 ccgggagctg tgcgacgacg gcacgctggc agtgaacgcc tggctcgtgc tactgcacaa
     9061 cagccgcctc ggacaaaccc acctcgacgc cacagtcgcc aacgccttcg gcgatcgtta
     9121 catctacagc ctctgtccgt ccgcaccggc ggcccgagag tacgcggtcg cactgtgcgc
     9181 aagcatcacc gacgagtacc cggtctccgg gttgcgcttg gagactcccg gcttcctccc
     9241 cttcgctcac ggctatcacc acgagttcgg cctcgttcgg caaaacgatt ggctcgagcg
     9301 cctgcttggc ctgtgcttct gccgacactg cgctgacggc gttcgcgacg ccggcatcga
     9361 gctcgacgga ctccgcgggc gggtggcgtc ggcgatcgag acgtacctcg cctctgacgt
     9421 cgactatccg gacgatatgg cggaggcgtt ctggttggca gacgtcgtac tcgattccga
     9481 cctcggcgca ttcctccggt ggcgttgcga cgtcgtcacg tcgctggtcg aggcgattcg
     9541 ggccgaggtt cccatgaacg ttgacgtcgc gatcattccg tcggttgcgc gccccagcgg
     9601 cggcgcctgg tacgagggca gcgaccttgc cgccctggca cgggccgccg ggggtgtcga
     9661 ggtttgcttc tacgaacacg gcgccgaccg cgtcgcagcc gacctgctgg acgtccagcg
     9721 tcggatcggc gacgccggtc ggatcagcgc ggttcttcga cccggccacc ccgacctcag
     9781 cgatcgaacg atggtcgccg cggcggttgc cacgctcgcc cgcggcggca tccacgacgt
     9841 cggcttctac aactacgggc acctgcgcgc ctctgcactc gattggatcg ccgaggcgtt
     9901 gtcagccacg gaggagcgag cgtgagcgac ttcaccgggc gggtggttct gatcaccggc
     9961 gcagccggtg gcatcgggca ggctctgtca cggctgttcg gtgaacgagg cgcagcgatc
    10021 gcggcgctgg acagagacga cgctgtcaac gatttcgctg ccgcgttgca cgatgaaggc
    10081 atcgagtcgg cggccgcggt cgccgacgtc ggcgcggcgg acgagatag
//
LOCUS       DBNGNK010000240         3602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1332072,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000240 DBNGNK010000000
VERSION     DBNGNK010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1332072"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..88
                     /locus_tag="AC150V_07105"
     CDS             <1..88
                     /locus_tag="AC150V_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005473425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HIR3762727.1"
                     /translation="ARVVVFCASDLSLLMTGSTLLVDAGELT"
     gene            complement(92..1006)
                     /locus_tag="AC150V_07110"
     CDS             complement(92..1006)
                     /locus_tag="AC150V_07110"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3762728.1"
                     /translation="MLFNAHVHMSQWERDFTQELANVHLAQFEGRTSWLTGEPWRPDG
                     WCVSGADLIRHMDEAGVDRALVMCLAYVPVGAFDPTMGDYIADLVDAYPERLVGFYSA
                     DPLGGADEAARLRHYVAERGLSGLKMLPSYNYASINDRRIWPMYEAAADLGVPMTIHT
                     GWSSLPAGKSLAYDHPLYIEDALVDFPGLRPILAHCGFAWSELVLMLLAKDARVAADF
                     AWWSQTQPPWRAAQTMSMAKYLGVLDRLFWGTDYPFTDFASDLAYWRSIPATSRRLGL
                     EPEVTDEDIDRFVGPNFAAFLAASPGPG"
     gene            complement(1047..1394)
                     /locus_tag="AC150V_07115"
     CDS             complement(1047..1394)
                     /locus_tag="AC150V_07115"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3762729.1"
                     /translation="MTRDETFPEFIRSLPVVDSPLASLRGWMLQTDQALAMFYEIPDG
                     VVLPEHSHGAQWGVVLAGAIDFTIGGETRTYRTGDTYYVPDGVAHSAVIHSGFVGIDV
                     FADADRYAARTDG"
     gene            complement(1391..2224)
                     /locus_tag="AC150V_07120"
     CDS             complement(1391..2224)
                     /locus_tag="AC150V_07120"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762730.1"
                     /translation="MSSEIEQPEPSYPPLASLVELSDKVALVTGAAQGFGFACARRLS
                     EAGAAVVLADRRADRLAEAHGRLAGDGRDVASAEGDVSIEADVQSLVSTAVSRFGRLD
                     VLVNNAGVFSNFLLEHLTHAEFQRILGVNVGGSFLCTRAAAAQMRKQGAGGSIINVSS
                     IDAVHPSGSGLSHYGTSKHAIWGLTKTMALELAPDGIRVNAIAPGPSLTEGAIEFVEA
                     GAPDGIDVDQQWAAYEERIPLRRLAHPDDVGRAALFLASDLSSYVNGAQIVVDGGLLA
                     A"
     gene            2347..3219
                     /locus_tag="AC150V_07125"
     CDS             2347..3219
                     /locus_tag="AC150V_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3762731.1"
                     /translation="MAVLLIGYDTESAAVGEGLARFLGPDVPQYRCALEPETTRRGVD
                     VLSRLHEELDAPCTFFVCGRTLLHALDALEPLAASSLFDIQQHTYSHVVFRDVRYRPI
                     GSEVEAVLPETPPVALREELAFTSELIRKYLGRECIGLRTPFGYYRGLRERPDLLDIV
                     AETGHRYVTSWGRNEENGNPTPWVQPFAYEEEGFPGILEIPFQFWLDGIWFDANGYGE
                     GRAFGQALRGAIDEVAEHDLVFATAFHEWCAVEANEEETGWIRALIEHARERDVQIMS
                     YSDYWRQVAGAPTD"
     gene            complement(3216..>3602)
                     /locus_tag="AC150V_07130"
     CDS             complement(3216..>3602)
                     /locus_tag="AC150V_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-dihydroxypyridine 5,6-dioxygenase NicX
                     family protein"
                     /protein_id="HIR3762732.1"
                     /translation="IEFVIEAGRIIDIRGEGYQAELVRSYMADFDDADAYGLSHIGWG
                     LDERAKWAALATDSRGHGMELRAFSGNVLFSTGPNDLVGGPNHTSCHLDIPMRNCSLW
                     CDDRPVLVDGDVVIDEMRPAGTPVHA"
BASE COUNT          590 a         1181 c         1274 g          557 t
ORIGIN      
        1 cgcacgcgtc gtcgttttct gcgcgagcga cctctcgctg ctcatgaccg ggagcaccct
       61 gctcgtggac gcaggcgagc tgacgtgacg gtcagcccgg tcccgggctg gcggcgagga
      121 aggcggcgaa gttcggcccg acgaaacgat cgatgtcctc gtcggtgacc tccggctcga
      181 gcccgaggcg ccgggaggtg gccgggatcg atcgccagta ggcgagatcg gaggcaaagt
      241 cggtgaacgg atagtccgtg ccccagaaga gtcgatccag cacgccgagg tacttcgcca
      301 tcgacatcgt ctgggcggcc cgccagggcg gctgtgtctg cgaccaccag gcgaagtcgg
      361 cggcgacgcg ggcgtccttc gccaacagca tgagcaccag ctccgaccag gcgaacccac
      421 agtgagcgag gatcggccgc agccccggga agtcgacgag cgcgtcctcg atgtagagcg
      481 ggtggtcgta cgcgagtgac ttgcccgccg ggagggagct ccagccggtg tggatggtca
      541 tcggcacgcc gaggtcggcc gcggcttcgt acatgggcca gatccggcga tcgttgatcg
      601 aggcgtagtt gtaggaggga agcatcttca ggccggacag cccgcgctcg gccacgtagt
      661 gccgcagacg ggccgcctca tccgcacctc ccagcgggtc ggcgctgtag aagccgacca
      721 ggcgctcggg gtaggcgtcc accaggtcgg cgatgtagtc acccatcgtc gggtcgaacg
      781 ctccgacggg gacgtacgca agacacatga cgagcgcacg gtcgactcct gcttcgtcca
      841 tgtggcggat caggtcggcc ccggagacac accacccgtc gggtcgccag ggctcgccgg
      901 tcagccacga cgtgcggccc tcgaactgcg ccagatggac gttcgcgagc tcctgtgtga
      961 agtcgcgctc ccactgactc atgtgtacgt gggcgttgaa gagcatcgat cctcctacgc
     1021 gcggcggggc gggtacacgc tgccggctag ccgtccgtcc gcgcggcgta gcggtccgcg
     1081 tcggcgaaca cgtcgatgcc gacgaagccg gagtggatga cggcgctgtg ggcgacgccg
     1141 tcgggcacgt agtacgtgtc gcccgtgcgg taggtgcgag tctccccgcc gatcgtgaag
     1201 tcgatcgccc ccgcgagaac cactccccac tgcgcgccgt gcgaatgctc ggggagaacc
     1261 acgccgtcgg ggatctcgta gaacatcgcg agagcttggt cggtctgcag catccagccg
     1321 cgcagcgagg cgagtgggct gtcgaccacc gggagcgacc ggatgaactc ggggaaggtc
     1381 tcgtctctgg tcatgcggcg agcaaccctc cgtcgacgac gatctgtgcc ccgttgacgt
     1441 acgagctgag gtcggacgcc aggaacaatg cggccctccc gacgtcgtcg ggatgggcga
     1501 gacgcctgag ggggatgcgc tcctcgtagg ccgcccattg ctggtcgacg tcgatcccgt
     1561 cgggggcacc tgcctcgacg aactcgatcg caccttccgt cagcgacggt ccgggggcga
     1621 tggcgttgac gcgaatgccg tccggtgcca gctcgagcgc catggtcttg gtcaggcccc
     1681 agatcgcgtg cttcgacgtc ccgtagtggg agaggcccga gccgctcggg tggacggcgt
     1741 cgatcgagct cacgttgatg atcgagccgc ccgcaccctg cttccgcatc tgcgccgctg
     1801 cggcgcgggt gcacaggaag gagccgccga cgttcacgcc gaggatgcgc tggaactcgg
     1861 cgtgggtgag atgctcgagg aggaagttcg agaacacgcc cgcgttgttc acgagcacgt
     1921 cgagacgccc gaagcgcgat acggcggtcg agacgagcga ttggacatcg gcctcgatcg
     1981 acacgtcgcc ctcggcggag gcgacatcgc gtccgtcgcc ggcgagccgc ccgtgggcct
     2041 cagccagcct gtcggcacgg cggtcggcga ggacgaccgc ggccccggcc tccgagaggc
     2101 gccgagcgca ggcgaacccg aagccctggg cggcgcccgt cacgagcgcg accttgtcgc
     2161 tcagctctac gagcgacgcc agcgggggat agctcggctc gggctgctcg atctcagacg
     2221 acacgaccgc acgcgcctcc gcggcgaagg acagggcgga tctacgttat aagatcatca
     2281 gagaacatgc gaggcgctcg ccggcgggcg cgcgcagagt cggagtacca aggaaaaggg
     2341 gcacgaatgg cggtgctgct catcgggtac gacaccgagt ctgctgcagt cggcgagggg
     2401 ctcgcgcggt tcctcggccc ggacgttccc cagtatcggt gcgctctcga gcccgagacg
     2461 acccgacgtg gcgtcgacgt gttgtcccgg ctccacgagg aactcgacgc gccgtgcacg
     2521 ttcttcgtct gcgggcggac gctcctgcac gcactcgatg cgctcgagcc cctcgcagcc
     2581 tcgtctctgt tcgacatcca gcagcacacg tacagccatg tcgtgttccg ggacgtgcgc
     2641 tatcgcccga tcggcagcga ggtggaggct gtgctcccgg aaacgccccc agtcgcgctc
     2701 cgggaggagt tggcgttcac gtccgagctg atccggaagt acctcgggcg cgagtgcatc
     2761 ggactgcgga caccgttcgg ctactaccgc ggtctgcgtg agcgtcccga cctgctcgac
     2821 atcgtcgccg agaccgggca tcgctatgtc acgtcgtggg gacgcaacga ggagaacggc
     2881 aaccccacgc cgtgggtaca gcccttcgcc tacgaggagg agggctttcc cggcatcctc
     2941 gagattccgt tccagttctg gctggacggg atctggttcg acgccaacgg ctacggcgag
     3001 gggcgcgcct tcggccaggc gctccggggc gcgatcgacg aggtcgccga gcacgatctc
     3061 gtcttcgcca ccgcgttcca cgaatggtgc gcggtcgagg ccaacgagga ggaaaccggc
     3121 tggatccggg ctctgatcga gcatgcccgg gaacgggacg tccagatcat gagctactcg
     3181 gactactggc gtcaggtggc cggcgcgccg acggactagg cgtgtacggg tgtgccggcg
     3241 ggccgcatct cgtcgatgac gacgtctccg tcgacgagca cggggcggtc gtcgcaccaa
     3301 aggctgcagt tgcgcatcgg gatgtcgaga tggcagctcg tatggttcgg cccgccgacg
     3361 agatcgttgg gcccggtgga gaagagcacg ttgccggaga aggcacgcaa ctccattccg
     3421 tggccgcggc tgtcggtggc gagtgccgcc cacttcgcgc gctcgtccag accccagccg
     3481 atatgcgaga gcccgtacgc gtcggcgtcg tcgaaatcgg ccatgtacga gcgtacgagc
     3541 tccgcctggt agccctcgcc gcggatgtcg atgatccgtc cggcctcgat cacgaactcg
     3601 at
//
LOCUS       DBNGNK010000241         2396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_258724,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000241 DBNGNK010000000
VERSION     DBNGNK010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_258724"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..102)
                     /locus_tag="AC150V_07135"
     CDS             complement(<1..102)
                     /locus_tag="AC150V_07135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3762733.1"
                     /translation="MSVSSEPVCLIAAGGTAGHVRPALAVGEALRRRG"
     gene            complement(155..1177)
                     /gene="ftsW"
                     /locus_tag="AC150V_07140"
                     /pseudo
     CDS             complement(155..1177)
                     /gene="ftsW"
                     /locus_tag="AC150V_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
     gene            complement(1174..>2396)
                     /locus_tag="AC150V_07145"
     CDS             complement(1174..>2396)
                     /locus_tag="AC150V_07145"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3762734.1"
                     /translation="LGLARSGQAAALALGSQGVVVVAHDSSKHVDAARVAAAGVEVHL
                     GTEAEALLEGIDVVIKSPGIPGEASPLPAARELGVPVWSEIELGARLLSNPLLGVTGT
                     NGKTTTSALIGAMFEAAGRPVEVAGNIGRPLTSLVGDVDEEAWVVCELSSFQLEDVET
                     LKPRIAVLLNLEPDHLDRHGSFNSYTQAKLRIFERQQAGDFAVLPRGFGPVGGDAVRV
                     EFAADDPLPAEPLIPGAHNRENAAAAAAAATVAGIGEPAIAEALRTFSGVPHRIEEVA
                     EIGGVRYVNDSKATNVAAARRAVASFSAPLHLILGGLGKNESYAPLASDLRPGTRVYL
                     IGAAADEIAGALDAAGVSYEQSGDLATATRSAAAAAAAGEVVLLSPACASYDQFSDFE
                     ARGDAFRELVEGLA"
BASE COUNT          410 a          836 c          828 g          322 t
ORIGIN      
        1 gccgcgtcgc cgcagagcct cgcccactgc gagagccggc ctgacgtggc cggccgttcc
       61 gccggctgcg atcaggcaca cgggctccga cgacacgctc attcaccgcg atgttaagga
      121 gcacgcccac ggacgcgagc agaacgacga gcgaggagcc gccgtacgag acgaaaggca
      181 gggggattcc ggtcagcggg gcgacgccga gaacggccgc gaggttcact gccgcctgcc
      241 cgcagacgag cgtcgtgatt ccggccgcga gccgcttccc gaacggatct ctgcagtcca
      301 gcgcgatccg gaagccggcc caggcgaaaa cgccgaaggc gcccgtcacg aggaccgacc
      361 cgacgagccc gagctcctcg ccgatgatgg cgaagatcat gtcggtgtgg gcctcgggta
      421 ggtagaagat cttctggacg ccctcgccga gcccctgtcc cgtgatcccg cccgagccga
      481 cgccgatgat cgcctgcacc gtctggaagc ccgccccctc agcgtcctgc catggatcga
      541 ggaagctgag gacgcgctcg cggcgatagg gctcggtcca gatcgcgagg acgccgagcc
      601 cgacggcgag cgagccggcg acgagcaaga gccgcccggg gaggccggag atcacgagca
      661 cccccgcgac catcacgaag agagtgatcg tcgtcccgag gtcgggctgg agcaggatca
      721 gaccgccgag cacgaccgtc gcgagcccga tcggcttcat cagctcgccc aaggtgcgag
      781 gtgccggtcg tcgggcgagg aaggcgcaga cccagacgca gaggcccagc ttcgccagct
      841 ccgacggctg gaagctggct ggaccgagga tgaaccaccg cttggcgccg ttgatttccg
      901 gagcgatcac gagcacagca acgcacagtg agaaagccgc gacgagcagc gccggggcaa
      961 aggcgcgaac gacgtggtag tcgaggcggg aaagaccgac gaggagaacc acgccgacga
     1021 cggcgtacgc tccctgcttg acgagaaacc gcatcgggtc gccctccccg agcgttgcgg
     1081 cggctgacgt cgcgctgaag accatcacga ggccgaacgc cacgagtccg agcgtgacga
     1141 ggacgagcag acgctgctcg agctgtccct tcgtcatgcg agtccctcca cgagctcccg
     1201 gaaggcgtcg ccgcgcgcct cgaagtcgct gaactggtcg tagctcgcgc aggcgggcga
     1261 caacaggacg acctcgcccg cggcggccgc cgcggcggcc gagcgggtcg cggtggcgag
     1321 gtcaccgctc tgctcgtacg agactcccgc agcgtcgagc gccccggcga tctcgtcggc
     1381 tgcggcgccg atcaggtaga cgcgcgttcc cgggcgaaga tcgctcgcca gcggcgcgta
     1441 gctctcgttc ttgccgaggc caccgaggat gaggtgcagc ggggccgaga atgaagccac
     1501 agctctccgt gctgcggcga cattggtcgc cttcgagtcg ttcacgtagc gaacgccgcc
     1561 tatctctgca acttcctcga tgcgatgggg cacgccggag aaggtgcgaa gcgcctcggc
     1621 aatggcaggc tcgccgatcc ccgcgaccgt cgcggcggcg gccgcggccg cagcgttctc
     1681 gcgattgtgg gcgcccggga tcaggggctc cgcgggcagc ggatcgtccg ccgcgaactc
     1741 gacccggacg gcatccccgc cgaccgggcc gaacccgcgc gggagcacgg cgaaatcacc
     1801 ggcctgctgc cgctcgaaga tgcggagctt ggcctgtgtg taggagttga agctgccgtg
     1861 ccggtcgaga tggtcgggct cgaggttgag gagaacggcg atgcggggct tgagcgtctc
     1921 cacgtcctcg agctggaacg aagacagctc gcagaccacc cacgcttctt cgtcgacgtc
     1981 tccaacgagc gacgtgagcg ggcgcccgat gttccctgcg acctcgaccg gtcgtccggc
     2041 cgcttcgaac atcgcgccga tgagtgcgct cgtcgtcgtc ttcccgttcg tgccggtgac
     2101 accgagcagg gggttgctga ggagcctcgc tccgagctcg atctcgctcc agaccggcac
     2161 gcccagctct cgtgccgccg gtagcggcga tgcctccccg ggaatccccg gactcttgat
     2221 cacgacgtcg atcccctcca gcagagcttc ggcctccgtt ccgagatgaa cttcgacgcc
     2281 tgcggcggcg acccttgcgg cgtccacgtg tttcgacgag tcgtgtgcga cgaccacgac
     2341 tccctgcgag cccagcgcca gcgccgccgc ctggcccgag cgtgcgagcc ccagca
//
LOCUS       DBNGNK010000242         6378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1404835,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000242 DBNGNK010000000
VERSION     DBNGNK010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1404835"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="AC150V_07150"
     CDS             complement(<1..345)
                     /locus_tag="AC150V_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR3762735.1"
                     /translation="MSVSVTQHKMFVGGDWVESASGESMEVLNPATGEPIAEVPRGTA
                     EDVDRAVAAAKTAWDEWRFKTPKDRMALLLGLADVIDDNAEELARLESLNVGKPYWVA
                     IDEPGAMSDNLRF"
     gene            430..1644
                     /locus_tag="AC150V_07155"
     CDS             430..1644
                     /locus_tag="AC150V_07155"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR3762736.1"
                     /translation="MRVLVVGTGGVGSAFAAIAQRRDFYERIVLADLDPSRPQAIVDR
                     LGEHDRFGSAGLDASDEDAVVRLIEDVKADAVLNATDPRFNPQIFDACLRARVTYLDM
                     AMTLSERHPVHPFVETHVKLGDYQFAQDENWRQAGLLALVGIGVEPGASDVFARHAAD
                     ELFSEIDEIGIRDGANLVIEGYDFAPTFSIWTTIEECLNPPVIWEKGRGWYTTEPFSE
                     PEVFDFPDGIGPVQCVNVEHEEVLLIPRWIDCRRVTFKYGLGDEFTDVLRTLRKLGLD
                     STETVRVRDLEVSPRDVVAATLPDPMTLGDRMLGKTCAGALVTGTGKDGNPRKVYVHH
                     VVDNEWSMREYASQAVVWQTALNPVVALELLQDGAWSGTGVLGPEAFPPQPFLDMLRE
                     YGSPHGHLELEP"
     gene            1709..1969
                     /locus_tag="AC150V_07160"
     CDS             1709..1969
                     /locus_tag="AC150V_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HIR3762737.1"
                     /translation="MALVRLRAPLRELAGGDSDHRVAGETVGEILAALEREYPAMAGW
                     VLDERGRIRRHVNVFVNGEYGREDTRLGPDDRVHVLPSISGG"
     gene            1974..3041
                     /locus_tag="AC150V_07165"
     CDS             1974..3041
                     /locus_tag="AC150V_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR3762738.1"
                     /translation="MTELLVGTRKGLFALRGSDGGAFDVAARCFEGVEVEYAMRDART
                     GRYLASVSNAFYGPKIWWTDDLAAEWQQAEGPALPEGDAALERIWVIVPGAADGLLYA
                     GGDPGVLFESRDGGESWELNRGLWEQPTRPEWRPGGGGLCLHTVVPWPGDPERLLVAI
                     SAVGVWLSEDGGQSWRHSNEGIVPRYVPEDARDNPIQHCVHDVRRASRQPDRLFMQFH
                     DGVYRSDDGGRHWIDIGSDTDLPSDFGFPVIVDPADPDSAFVIPLVGAGDRTTPGGVV
                     TVWETRDAGASWAPRNKGLPTRDAYLTILREAFDSEGEGDSLGLYFGATSGDVFGSLN
                     GGASWFGVASRLPPVYSVRVA"
     gene            complement(3049..3456)
                     /locus_tag="AC150V_07170"
     CDS             complement(3049..3456)
                     /locus_tag="AC150V_07170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR3762739.1"
                     /translation="MAELSTTQLAFLDKPFVGALTDLRPDGSPHTTVVWVDVTDGGLS
                     FNTARGRAKPRYIEADSRVSLLVVDPQNAYRWLSVSGTAELVDEGADEQIDRLAKKYL
                     GEDSYPWRQPGEQRVSVRITPERIDSFGLDAIA"
     gene            complement(3558..4469)
                     /gene="nadA"
                     /locus_tag="AC150V_07175"
     CDS             complement(3558..4469)
                     /gene="nadA"
                     /locus_tag="AC150V_07175"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR3762740.1"
                     /translation="MQEEVRELARERGAVILAHNYQVPEVQDAADYVGDSLGLSRQAA
                     STDAAAIVFCGVHFMAETASILCPKKTVLIPDPDAGCSLAASITADELREWKAQHPGA
                     VVVSYVNTTAEVKAESDYCCTSGNARAVIEAIPAEQEILFLPDLYLGLWLEQVTGRKL
                     TIWLGECHVHAGIRPADIERWQTEAPDAELLVHPECGCASQCMAFGNGRTHILSTEGM
                     IDFAAQSEKRRFLVATEVGILHRLNRESPGKVFEPVSESAVCQYMKMITLEKLRDSLR
                     DWKFQVEVPTETAARARQAIERMIAIV"
     gene            complement(4490..5308)
                     /gene="nadC"
                     /locus_tag="AC150V_07180"
     CDS             complement(4490..5308)
                     /gene="nadC"
                     /locus_tag="AC150V_07180"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003390285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR3762741.1"
                     /translation="MELTAVDVRSWLAEDIGAGDVTSEAVIPAGTRCSARLLLKEPGV
                     ICGLSVVEAVFRELDAAVEFEAHAVDGDVLEGGELAAVTGDTRGILAGERLALNILGR
                     LSGIATLTRRFVDEVAGTDAVILDTRKTTPGLRALEKEAVRRGGGTNHRLGLHDAVLI
                     KDNHLRLAGGITAALARAETHSALPVEIECESLEQVREAVAAGPDRILLDNMTSDQLA
                     RAVAVVDGRAELEASGGITLENVRCVAEAGVDFISIGALTHSARALDVSLEVLT"
     gene            complement(5299..>6378)
                     /locus_tag="AC150V_07185"
     CDS             complement(5299..>6378)
                     /locus_tag="AC150V_07185"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR3762742.1"
                     /translation="GLGLEGGHSRRRVHHVGGATTGLRISERLTARATAEPAVDLLVG
                     QRAVELLPGEGVRTNRDDVPAPAVLLASGGYSALWARTTNPPASVGDGIVLAYESGAA
                     VADLELVQFHPTALEESGLLLSEALRGEGALLLDEAGRRFTDELAPRDVVAREVGSRG
                     TALLDLRPIDRGRFPSLMEQLAAAGYDPAAEPVPISPAAHYTMGGIATDVDGRTEVPG
                     LYAAGECACTGVHGANRLASNSLLECLVFGRRAALAALHDDTTSTAAGLTAPGLPEDS
                     GAVTDSVRRAVWEHAGLVRDAAGLEQLLDSPVRLARLLAQSALSRTESRGGHFRADFP
                     IEDPALAGMHTVLRPGHAPELERWN"
BASE COUNT          963 a         2192 c         2211 g         1012 t
ORIGIN      
        1 gaagcgcagg ttgtcggaca tcgccccggg ctcgtcgatc gcgacccaat acggcttgcc
       61 gacattgagc gactcgagcc gcgcgagctc ctcggcgttg tcgtcgatga cgtcggcaag
      121 cccgagcagc agcgccatgc ggtccttcgg tgtcttgaac cgccactcgt cccatgcggt
      181 tttcgccgcc gccacggcgc gatcgacatc ctcggccgtc ccgcgcggca cctcggcgat
      241 cggctcaccc gtcgcagggt tgagaacctc catcgactcg ccggacgccg actcgaccca
      301 gtcgcctccg acgaacatct tgtgctgcgt gacggaaacg ctcatctcgc tcctccgtgt
      361 gctgggatcc ggcgcgagcg tagcaccgtg atctcggcgt ccaccacggg tttgctgtag
      421 aacccgccca tgcgggtcct cgtcgtcggg accggtgggg tcgggtcggc attcgccgcg
      481 atcgcccagc gtcgtgattt ctacgagcgg atcgtcctgg ccgacctcga cccgtcgcgg
      541 ccgcaggcga tcgtcgacag gctcggggag catgaccgct tcggctcggc cggcctcgat
      601 gcgtcggacg aggatgctgt cgtacggctg atcgaggacg tgaaggcgga cgcagttctg
      661 aacgccaccg acccgcgctt caacccgcag atcttcgacg cctgcctccg cgcccgcgtg
      721 acctacctcg acatggcgat gacgctgtcc gagcgacatc cggtgcaccc gttcgtcgag
      781 acgcacgtga agctcggcga ctaccagttc gcgcaggacg agaactggcg ccaggcgggc
      841 ctactcgcac tggtcgggat cggcgtcgag ccgggagcct ccgacgtgtt cgcgcggcac
      901 gccgccgacg agctcttctc ggagatcgac gagatcggca ttcgggacgg cgccaacctg
      961 gtcatcgagg gatacgactt cgcgccgacg ttctcgatct ggacgaccat cgaggagtgc
     1021 ctgaaccctc ccgtgatctg ggagaagggc cgcggttggt acacgaccga gccgttctcc
     1081 gagccggagg tgttcgattt cccggacggc atcggtccgg ttcagtgcgt caacgtcgag
     1141 cacgaggagg tcctgctcat cccgcgctgg atcgactgtc gcagggtcac cttcaagtat
     1201 ggcctcggcg acgagttcac cgacgtcttg cgcacgctgc gcaagctcgg gctggactcg
     1261 accgagacgg ttcgcgtgcg tgatctcgag gtgtcgcccc gtgacgtcgt cgccgcaacg
     1321 ctgcccgacc cgatgacgct cggcgaccgc atgctcggga agacgtgcgc cggagcgctg
     1381 gtcaccggaa ccggcaagga cgggaacccg cgcaaggtgt acgtccacca tgtcgtcgac
     1441 aacgagtggt cgatgcgaga gtacgcgtcg caggccgttg tctggcagac ggcgctcaat
     1501 cccgtcgtcg cgctggaact cctgcaggac ggtgcctggt cggggacggg cgtgctcggc
     1561 ccggaggcct tcccgccgca gcccttcctc gacatgctcc gcgagtacgg ctcgccacac
     1621 gggcacctcg agctcgagcc gtagcggcgg tcgatttcgg gaggtcccgt tcgtccagct
     1681 aggcgatgag acgtggcagg atgcaccgat ggctctcgtg agactgcgcg cgccgctgag
     1741 ggagttggcg ggcggcgact cggatcacag ggtggccggc gagacggtag gcgagatcct
     1801 cgccgcgctc gagcgcgagt atccggctat ggccggctgg gtcctggacg agcgaggccg
     1861 catccgccgc cacgtcaacg tcttcgtcaa cggcgaatac ggacgggagg acacgcggct
     1921 cgggccggac gaccgggttc acgtgcttcc ctcgatttct ggaggctgag cacatgacgg
     1981 aacttcttgt cggcacacgg aaaggtctgt tcgctctcag aggctcggac ggcggcgctt
     2041 tcgacgtcgc ggcgcggtgc ttcgagggcg tcgaggtcga gtacgcgatg cgggacgccc
     2101 gcaccggtcg ctatctcgcg tcggtctcga acgccttcta cggaccgaag atctggtgga
     2161 ccgacgatct cgcggcagag tggcagcagg ccgaggggcc ggcactgccc gaaggcgacg
     2221 ccgcgctcga gcggatctgg gtgatcgtgc cgggcgcagc cgatggcctc ctctacgcgg
     2281 gcggcgaccc gggcgtcctc ttcgagagcc gcgacggcgg cgagagctgg gagctgaacc
     2341 gagggctttg ggagcagccg actcgacccg agtggcgccc agggggcggc ggtctctgcc
     2401 tgcacacggt cgttccgtgg ccgggagacc ccgagcggct gctggtcgcg atctctgcgg
     2461 tgggcgtctg gctctccgaa gacggcggcc agtcgtggcg ccattcgaac gaggggatcg
     2521 ttccccggta cgtcccagag gacgcacgcg acaatccgat ccagcattgc gtgcacgacg
     2581 tgcgacgggc gtcgcgacag cccgacaggc tgttcatgca gttccacgac ggcgtctacc
     2641 gctcggacga cggcggccgg cactggatcg acatcggcag cgacaccgat ctgccctctg
     2701 acttcggctt ccccgtcatc gtcgatccgg ccgaccccga cagcgccttc gtcatcccgc
     2761 tcgtcggagc aggcgatcgc acaacgcccg gcggcgtcgt caccgtctgg gagacgcgcg
     2821 acgcaggcgc aagctgggcg ccgcgcaaca agggcctccc gacacgcgac gcgtatctga
     2881 cgatcctgcg cgaggcgttc gactcggagg gcgagggcga ctcgctcggc ctctacttcg
     2941 gcgccacctc gggggacgtg ttcggctccc tgaacggtgg cgcgagctgg ttcggcgtgg
     3001 cgagccgtct accgccggtc tactccgtcc gggtggcctg agcagggctc aggcgatcgc
     3061 gtcgaggccg aaggagtcga tccgctccgg ggtgatccgg accgagactc gctgctcgcc
     3121 gggttgccgc cagggatagg agtcctcgcc gagatacttc ttcgcgaggc ggtcgatctg
     3181 ctcgtcggcg ccctcgtcga cgagctcggc cgtgccgctc acgctcaacc agcggtacgc
     3241 gttctgcgga tcgaccacga gcagcgagac gcgcgagtcg gcttcgatgt agcgcggttt
     3301 ggcccgtccg cgagcagtgt tgaagctgag accgccgtcg gtgacgtcga cccagacgac
     3361 ggtggtgtgc ggcgacccgt cgggtcgcag gtcggtgagc gcgccgacga agggcttgtc
     3421 gaggaatgcg agttgcgtcg tgctcagctc tgccattggg gtctcctccc gttcgcagtg
     3481 agcaagtaac agactgttac tagccggcgt gagggtaaac gcgccgggcc cgctcgttca
     3541 tcccgagcct ccgggcgtca cacgatcgcg atcatgcgct cgatcgcctg ccgagcccgg
     3601 gcggcggtct ctgtaggcac ctcgacctgg aacttccagt cccgcagcga gtcccgcagc
     3661 ttctccagcg tgatcatctt catgtactgg cagacggcgc tctcggagac cggctcgaac
     3721 accttgcccg gcgactcccg gttcaggcgg tgcagaattc cgacctctgt ggcgaccagg
     3781 aagcgccgct tctcggactg cgcggcgaag tcgatcatcc cctccgtcga gaggatgtgt
     3841 gtgcgcccgt ttccgaatgc catgcactgg ctggcgcaac cgcattcggg gtgcaccagc
     3901 aattcggcgt ccggggcctc cgtctgccag cgctcgatgt cggcgggacg gatgcccgcg
     3961 tggacatggc actcgccgag ccagatggtg agcttccgtc cggtgacctg ctcgagccag
     4021 agcccgaggt agaggtcggg aaggaagagg atttcttgct cggcgggaat tgcctcgatc
     4081 accgcccggg cgttgccgga ggtgcagcag tagtcggact cggccttcac ctcggcggtc
     4141 gtgttgacgt aggagacgac cacggcgccc ggatgctggg ccttccactc gcgcagctcg
     4201 tcggcggtga tcgacgcggc cagcgagcaa cccgcatcgg gatccgggat cagcaccgtc
     4261 ttcttggggc agaggatcga agctgtctct gccataaaat gaactccgca gaagacgatc
     4321 gctgccgcgt cggtcgacgc ggcctgccgc gacaggccga gggagtcgcc gacgtagtcg
     4381 gcggcatcct gcacctcggg cacctggtag ttgtgcgcga ggatcacggc gccgcgctca
     4441 cgcgcgagct cgcgcacctc ctcttgcatg gtcgtaatct cggcagcggt cacgtcagca
     4501 cctccagcga tacgtcgagg gcgcgggccg agtgagtgag cgccccgatc gagatgaagt
     4561 ccactcctgc ctccgccaca cagcggacgt tctcgagcgt gatgccgccc gacgcctcca
     4621 gctccgcgcg gccgtcgacg accgccacgg cgcgggcgag ctggtcgctg gtcatgttgt
     4681 cgagcaggat cctgtccgga ccggccgcga ccgcctctcg cacctgctcg aggctctcgc
     4741 attcgatctc gaccgggagg gccgaatgcg tctcggcgcg cgcgagcgcg gccgtgatac
     4801 cgccggccag cctgagatgg ttgtccttga tcagcacggc atcgtgcaga cccagacggt
     4861 ggttcgtccc gccacctcgg cgcacggcct ccttctcgag cgctcggagc cccggggtcg
     4921 tcttgcgagt gtcgaggatc acggcgtcgg tgccggcgac ctcgtcgacg aaccggcgcg
     4981 tcagcgtcgc gatccccgac aggcgtccga ggatgttcag cgccagacgc tcgccggcga
     5041 ggatgccccg ggtgtccccc gtcacagctg ccagctcgcc gccctcgagc acgtcgccgt
     5101 cgacagcgtg ggcctcgaac tcgaccgcgg cgtcgagctc gcggaagacg gcctcgacga
     5161 cgctcagtcc gcagatcact cccggctcct tgagcagcag tcgggccgag caccgcgtcc
     5221 cggcggggat aacggcctcg ctcgtcacat cgccggcgcc gatgtcctcg gcgagccagg
     5281 agcgaacgtc gacggcggtc agttccatcg ttcgagctcc ggcgcgtggc ccggacgaag
     5341 gacggtgtgc atcccggcga gggcgggatc ctcgatcggg aagtccgcac gaaagtgacc
     5401 cccgcgcgac tccgtccgag agagagcgga ctgggcaagg aggcgagcaa gccgcacggg
     5461 cgagtcgagc agctgctcga gacctgcggc atcgcgcacg agccccgcat gctcccagac
     5521 ggcgcgccgc acggaatcgg tcactgcgcc ggagtcctcg ggaagcccgg gagccgtgag
     5581 cccggccgcc gtcgacgtcg tgtcgtcgtg cagcgcggcg agcgccgccc gccggccgaa
     5641 cactaggcat tcgaggagcg agttggaagc gagtcggttg gcgccgtgaa cgccggtaca
     5701 ggcgcactcg ccggcggcgt agagccccgg aacctcggtg cgcccgtcga cgtcggtcgc
     5761 gatgcccccc atcgtgtaat gcgcggccgg cgagatgggc acgggctccg ctgcggggtc
     5821 gtagccggcg gcggcgagct gctccatcaa ggaggggaac cggccccggt cgatcgggcg
     5881 gaggtcgagc agcgccgttc cgcgcgatcc gacttcgcgc gcgacgacat ctctcggtgc
     5941 gagctcgtcc gtgaagcgcc gccccgcctc gtcgagcagc aacgcgcctt ctccgcgcag
     6001 cgcctcgctc agcagcagcc ccgattcctc gagcgccgtc ggatggaact gcacgagctc
     6061 gaggtctgcc accgcagctc cgctctcgta cgcgagcacg atcccatctc cgacggacgc
     6121 gggcgggttc gtcgtccggg cccagagcgc cgagtagccg ccggacgcca ggaggacagc
     6181 cggcgcaggt acgtcgtcgc ggttcgtccg taccccctcc cccggaagca gctcgaccgc
     6241 gcgctgcccg accagcagat cgaccgccgg ctcggctgtc gcgcgggccg tcaggcgctc
     6301 cgagatgcgc aagcctgtcg tggctccgcc gacgtggtgc acgcgccggc gagaatggcc
     6361 gccttcgagc cccaggcc
//
LOCUS       DBNGNK010000243         3980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_545103,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000243 DBNGNK010000000
VERSION     DBNGNK010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_545103"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1603)
                     /locus_tag="AC150V_07190"
     CDS             complement(212..1603)
                     /locus_tag="AC150V_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005548473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR3762743.1"
                     /translation="MSTEEIGLAAATIWVLVAAFLVIFMQAGFALLEAGLTRMKNVGH
                     IAAKNALVLAIAALVFWAVGFGIAFGDGGNGFVGGSGFLPSADDLIAVGSAPFSWFGS
                     IPAAAALVFQTAFAAVSLAIVWGAMAERTRLWVYIVFGAAYLFVYSIVAHWIWSPDGW
                     LFGLGMQDFAGSTVVHYQGALAGLAGALLLGARIGKYGADGKPNAIPGHNMAYVTVGV
                     FILWFGWFGFNAGSTLSVDYGGVGFFAYVALNTLVAAAAGVAGAMIASWLLIKKPDLS
                     MMLNGAIGALVAITAACAFVAPWAAVLIGFVAGTVVVGGILLVERLRIDDPVGALAAH
                     GMSGVWGTLALGFFAVPALAERLDWGRAGLFYGGGARQLGVQALGLLAVGAFTFTASF
                     AILWVMKVAVGIRTEPEVERAGLDVSEHGMWGYPEFYIPVPGGYGTEHDTHVTHPART
                     RAEPPPEPAPAAS"
     gene            complement(1600..1947)
                     /locus_tag="AC150V_07195"
     CDS             complement(1600..1947)
                     /locus_tag="AC150V_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012753.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3762744.1"
                     /translation="MKVEAVVVRDRVETVMEAVEEHTGHVGVTVIEAVGHGREKGLTH
                     EYRGRVFESRFLPKAHLTFVVRDEIAEDVLAAIVDSSRTGHLNGDGICWMSPVHNVTH
                     NRTGQRLEEVETV"
     gene            2225..3061
                     /locus_tag="AC150V_07200"
     CDS             2225..3061
                     /locus_tag="AC150V_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762745.1"
                     /translation="MTQIDVAPVRVDESEPPLAVDVRDLSKDFRRRRARRSGGAALAS
                     VTFSVTRGECVAILGQNGSGKSTLVRVLSTLILADGGSAQMFGLDVVADARAVRRLVN
                     RVSVEASFFKKLSAAENLHYAARFYGLTASRTRGRIPEILARVGFPPDRRGEPMENLS
                     RGMQQKVALARALLTSPVLLLLDEPTTGLDPRSKLEVQAFVREVRAAHDATILLCTHD
                     LAEAEALADRVGILHGGELLCLEPVGDLTRRYGSETLEEAFFAATGRDFADERPDEEG
                     RR"
     gene            3058..3891
                     /locus_tag="AC150V_07205"
     CDS             3058..3891
                     /locus_tag="AC150V_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3762746.1"
                     /translation="MRGLAVTIRHELIGLGGIVERNVYLTRRYIWWDVAFFLWTVANT
                     LTIVFIAEGIEATGGSIDTERVTTTLLIGAVVWAYLGIIFEFLTETVAWERWEGTIEY
                     TFMAPLSRAMHLGGTGVFAVLYGLLRAVLLFVVVALFFDLQLAGANFAAALVILFVAS
                     VSFFGIGMMTSVLPLLSPEKGGQLGYIAQGLLLVVSGVYYPVSVLPDWMQWLATISPA
                     TYALEGIRAAVLDGAELTSLWAQIWPLLVIGVVSIPLGLWVFWLGERHAKRRGKLKRS
                     G"
BASE COUNT          629 a         1404 c         1312 g          635 t
ORIGIN      
        1 cacggtcgtg cggtcgcggg gtctcatgga cgtgtccgaa cgcggcatca cattcctctg
       61 aggtccagcg gaggggccga gaggggtgag cgacctcgaa gcccgtccac ggcggccact
      121 acaaggccgc cctgatcgga catggctgcc ggaggcgcct aagctcttgg gcggcggggc
      181 gccctggggg acgccccgcc gtcgcttccg gctagctcgc cgccggcgca ggctcgggcg
      241 gtggttccgc cctcgtacgc gccggatgcg tgacgtgggt gtcgtgctcg gtcccgtagc
      301 caccgggaac ggggatgtag aactcgggat atccccacat gccgtgctcg gagacgtcca
      361 ggcctgccct ctccacctcg ggctccgtgc ggatcccgac ggcgaccttc atcacccaga
      421 ggatcgcgaa cgacgcggtg aacgtgaaag ccccgactgc cagcagaccg agagcctgca
      481 cgccgagctg gcgcgctcca ccgccgtaga agagaccggc ccgcccccag tcgagcctct
      541 ccgcgagcgc cggcacggcg aagaagccga gtgcgagcgt tccccagacc cccgacatgc
      601 cgtgcgccgc gagtgcgccg acggggtcgt cgatgcggag ccgctcgacg agcaggatgc
      661 cgccgacgac gaccgtcccg gcgacgaagc cgatcagcac agccgcccac ggcgcgacga
      721 aggcgcacgc ggccgtgatt gcgacaagcg ctccgatcgc tccgttcagc atcatcgaga
      781 ggtcgggctt cttgatcagc agccaggagg cgatcatcgc tcctgccaca ccggcggccg
      841 ctgcgaccag cgtgttcagc gcgacgtagg cgaagaagcc gacgccgccg tagtcgacgc
      901 tgagcgtcga gccggcgttg aagccgaacc agccgaacca gaggatgaag acgccgaccg
      961 tgacataggc catgttgtgg ccgggaatcg cgttgggctt cccgtcggct ccgtacttgc
     1021 cgatgcgcgc ccccagcagc agcgcgccag cgagaccggc cagcgcaccc tggtagtgga
     1081 cgaccgtcga accggcgaag tcctgcatcc cgaggccgaa gagccagccg tccggactcc
     1141 agatccagtg cgcgacgatc gagtagacga acaggtaggc ggcaccgaac acgatgtaga
     1201 cccagagccg cgtccgctcc gccatcgcgc cccacacgat cgccagcgag acggccgcga
     1261 acgccgtctg gaagacgagt gccgcggcgg ctgggatgga gccgaaccac gagaacggcg
     1321 ccgagcccac agcgatcagg tcgtccgcgg agggcaggaa cccggatccg ccgacgaagc
     1381 cgttgccgcc gtcgccgaat gcgatcccga agcccaccgc ccagaagacg agcgccgcga
     1441 ttgcgagaac cagtgcgttc ttggcggcga tgtgacccac attcttcatt cgcgtcagac
     1501 ccgcctcgag caaggcgaat ccggcctgca tgaagatcac gaggaacgcc gcgaccagca
     1561 cccagatggt cgccgccgcc agcccgatct cctcggtgct cataccgtct ccacctcctc
     1621 gagccgctgc ccggtccggt tgtgtgtcac gttgtggaca ggcgacatcc agcagatgcc
     1681 gtccccgttc aggtggcccg tccgcgacga gtccacgatc gcggcgagga cgtcttccgc
     1741 gatctcgtcg cgcacgacga atgtcaggtg cgccttcggc aggaaccgcg actcgaacac
     1801 ccgtccccgg tactcgtgcg tcaggccctt ctcacgcccg tgtccgaccg cctcgatcac
     1861 cgtcacgccg acgtgcccgg tgtgctcctc gaccgcctcc atcacggtct ccacgcgatc
     1921 ccgtacgacg acggcctcga ctttcatcat cgccatctca ccgcctcgtg catctctcct
     1981 ccctcgcttt ggtccaacaa aaaaagcccc aggtctcccg agggagaact gaggcgtcat
     2041 tgccttcggc gggggccgtc gtcggccgct gcctgccggc agggtagccg cgtggcggcg
     2101 cactcgcaag cctgctaccc tgcccggcct gatgttcgtt cgtaactagg acacccgttg
     2161 ccgcggccct cccgccgagg gccctttgca gtcctggttc acgtcttttc gaaggagcat
     2221 cagcatgacc cagatcgatg tcgcacccgt ccgggtagac gagagcgagc ctccgctcgc
     2281 cgtggacgtg cgcgacctca gcaaggactt tcgccgccgt cgcgcgcgga gaagcggcgg
     2341 tgctgctctg gcaagcgtca cgttctccgt cacgcgcggt gagtgcgtag cgatcctcgg
     2401 ccagaacggc tccggcaagt cgacgctcgt ccgcgtcctg tcgacactga tcctcgcgga
     2461 cggaggcagc gcgcagatgt tcggcctcga cgtcgtagcc gacgcgaggg cggtccgtcg
     2521 gctcgtcaac cgcgtctcgg tcgaggcgtc cttcttcaag aagctctccg cagcggagaa
     2581 cctccactac gctgcgcgct tctacggcct gaccgcgtcc cggacgaggg ggcggatccc
     2641 ggagatcctc gcccgcgtgg gcttcccgcc cgaccgtcgc ggcgagccga tggagaacct
     2701 ctcccgcggc atgcagcaga aggtcgcgct cgcacgcgcg ctcctgacct caccggtgct
     2761 gctgctgctc gacgagccga caacggggct cgacccccgc tcgaagctgg aggtgcaagc
     2821 cttcgttcgc gaggtgcggg cggcgcacga cgcgacgatc ctgctctgca cgcacgatct
     2881 cgccgaggcg gaggcgctcg cggaccgggt cgggatcctg cacgggggcg agttgctctg
     2941 cctcgagccc gtgggcgacc tgacgcggcg ctatggctcg gaaacgctgg aggaggcctt
     3001 cttcgcggct accggtcgcg acttcgctga cgagcgaccc gacgaggagg ggcggcgatg
     3061 agggggctcg ccgttacgat caggcacgag ctgatcgggc tcggcggcat cgtcgagcgt
     3121 aacgtctacc tgacccgccg ctacatctgg tgggacgtcg cgttcttcct ctggacggtc
     3181 gcgaacacgc tcacgatcgt cttcatcgcc gaggggatcg aggcgaccgg cgggtcgatc
     3241 gacaccgaac gtgtgaccac gacgctgctc atcggcgccg tcgtctgggc gtacctcggg
     3301 atcatcttcg agttcctcac cgagaccgtc gcctgggagc gctgggaggg gacgatcgag
     3361 tacacgttca tggctcctct gtcccgcgcc atgcacctcg gcggaacagg agtcttcgcg
     3421 gtgctgtacg ggctcctccg cgccgtgctg ctcttcgtcg tggtcgcact cttcttcgac
     3481 ctgcagctcg cgggcgcgaa cttcgccgct gcgctcgtga tcctgttcgt cgcgtccgtg
     3541 tcgttcttcg ggatcgggat gatgacctcg gtgctgcccc tactctcgcc ggagaagggc
     3601 ggccagctcg ggtacatcgc gcagggcctc ctcctggtcg tctcgggcgt ttactacccg
     3661 gtttcggtcc tgcccgactg gatgcagtgg ctcgccacga tctcaccggc gacgtacgcg
     3721 ctcgagggga ttcgcgccgc ggtcctcgac ggcgcggaac tcacttcgct ctgggcccag
     3781 atctggccgt tgctcgtgat cggagtagtc tcgatcccgc tcggactctg ggtcttctgg
     3841 ctgggcgagc gccatgcgaa gcgtcgcggc aagctgaagc ggagcggctg atgatcgagc
     3901 tcagagctgt cgaaacggac gaggacttcg aggcgtggcg ccgcgtccgt ctcaccgtgc
     3961 tcccgaacga gcgcgcagcg
//
LOCUS       DBNGNK010000244         2936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1260408,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000244 DBNGNK010000000
VERSION     DBNGNK010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1260408"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC150V_07210"
     CDS             complement(<1..371)
                     /locus_tag="AC150V_07210"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3762747.1"
                     /translation="MDVGLTMPTHGLLVRDEQDFFLQRLEPADMRPVEVAQRAERLGY
                     HSVWFSDHVCMGRDEGAHHTANVSGTRAYPNQAVMLDLVATMGAVAAATTTLRMAPSV
                     WIAPYRHPLSAAHQLATVDVLS"
     gene            complement(374..1909)
                     /locus_tag="AC150V_07215"
     CDS             complement(374..1909)
                     /locus_tag="AC150V_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR3762748.1"
                     /translation="MVDRIKADELDTLANASGYTLDGDERIEIQRIVEAIIETIELVD
                     TQDPELPAPIEAVRDPGRRPAPGEDPLNAIVRYCLVRADAAEGLLSGVRVAVKDAVAI
                     AGIPLTGGSRVLQGFVPTADSVVADRILRAGGEIVAITNMDDLAFSGGGDSSWYGPTL
                     NPWDEARTAAGSSGGSAAALFYDGIDVAIGGDQGGSIRAPASWCGVLGLKPTHSLVPY
                     TDIVGIDQTFDHCGPMAGSTAELAALLQAIAGADSSDPRQRDVRKADYLEAVASAPDH
                     LEGLRIGIVAEGFAADVGAEPEVVAAVRGAIERLRTIGAEPVDVSLPEHPRSGGVSFA
                     GFVEGMTNLLTSGGNGYAWAGRYWEELAPALAGGLRDHAQELSPQVKATLVVGRYLRE
                     RYASVLYARAQNLRPWLRSAYERALTGVDVLLLPTTPGRPHLVDPSLPISDRVLRGWA
                     NLANTAPTNMTGHPALSLPLAEADGLPVGVMLVARHFEDDRLLSVAATCERALGWRPA
                     RGA"
     gene            complement(1915..2463)
                     /locus_tag="AC150V_07220"
     CDS             complement(1915..2463)
                     /locus_tag="AC150V_07220"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3762749.1"
                     /translation="MAIDEAGSDSDVARRARDSEEVTMNSLEHQVAKLLATEEIRNLK
                     ARYADVCDTGYDPDRMRPFFTDDAVWDGGRFGRYEGLDAVAAFFAGVSAQITWALHYM
                     IAPMIEVQDDLAAARGTWYLWQPCTVVTPDGPRAVLLTGRYADRYRKEDGVWKFSEVL
                     LDCQTISPIEEGWVRRRFWDEG"
     gene            complement(2631..>2936)
                     /locus_tag="AC150V_07225"
     CDS             complement(2631..>2936)
                     /locus_tag="AC150V_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762750.1"
                     /translation="AAVGNSIWRLVYKNQTILLVTQTTKLPGGMILAQKRFHADWFGG
                     GGLKQPMLRSFSLPVKRGTGAYFGAKGSLTVSSLMTNLKGKLTETFVYDLKLPAART"
BASE COUNT          457 a          990 c         1031 g          458 t
ORIGIN      
        1 gagaggacgt cgacggtcgc gagctggtgc gcggccgaga gcgggtgccg gtacggcgcg
       61 atccagacgg agggagccat ccgcagtgtg gtcgtcgcag cggcgacggc gcccatggtt
      121 gcgacgaggt cgagcatcac ggcctggttg gggtaggcgc gggtgcccga tacgttcgcg
      181 gtgtggtgcg cgccctcgtc gcggcccatg caaacgtggt cggagaacca gaccgagtgg
      241 tagccgagcc gctcggctcg ctgagcgacc tcgacggggc gcatgtccgc aggctccagt
      301 cgttgcagga agaagtcctg ctcgtcgcgc acgagcagcc cgtgtgtcgg catcgtcagg
      361 ccgacgtcca tcgtcacgct ccccgcgcgg gccgccagcc cagggcgcgc tcgcatgtgg
      421 ccgcgaccga cagcagccga tcgtcttcga agtggcgggc gacgagcatg acgccgaccg
      481 gaagcccgtc cgcctcggcg agtggcagcg acagggcggg atgtccggtc atgttggtgg
      541 gagcggtgtt ggccaggttg gcccagccgc gcaatacgcg atcggagatc gggagcgacg
      601 gatcgacgag gtgcggccgc ccgggcgtgg tcgggagcag caggacgtcg acaccggtaa
      661 gcgcccgctc gtacgcgctg cgcagccagg ggcggaggtt ctgcgctcgc gcgtacagga
      721 cgctcgcgta gcgctcgcgc agataccggc cgaccacgag tgtcgccttg acctgcggcg
      781 agagctcctg cgcgtggtcg cggagcccgc cggcgagcgc cggggcgagt tcctcccagt
      841 agcgccccgc ccacgcgtaa ccgttgccgc cgctcgtaag gaggttcgtc atgccctcca
      901 cgaagccggc gaacgagacg ccgcccgatc gagggtgttc cggcagagag acgtcgacgg
      961 gctccgctcc gatcgtgcgg aggcgctcga tggcgccgcg aacggcggcg acgacctcgg
     1021 gctcggcgcc gacgtcggcc gcgaagccct cggcgacgat gccgatccgt aggccttcga
     1081 ggtgatcagg tgcggacgcg accgcctcga ggtagtcagc cttccgcacg tcgcgctgcc
     1141 gcggatcgga cgagtcggcc cctgcgatcg cctgcagcag cgccgcgagc tctgccgtcg
     1201 aaccggccat cgggccgcag tggtcgaagg tctggtcgat gccgacgatg tccgtgtacg
     1261 gaacgagcga gtgcgtcggc ttgaggccga ggacaccaca ccaggaagcc ggggcgcgga
     1321 tcgagcctcc ctgatcgccg ccgatcgcca cgtcgatgcc gtcgtagaag agcgccgcag
     1381 ccgagccccc cgacgagccg gcagccgtac gcgcctcatc ccacggattg agggtcggcc
     1441 cgtaccagct cgagtcgcca ccgcccgaga aggcgaggtc gtccatgttc gtgatcgcga
     1501 cgatctcacc gccggcgcgc aggatgcggt cggcaacgac gctgtcggcc gtcgggacga
     1561 agccctggag gacgcgcgat ccgcctgtga gcgggatccc ggcgatcgcg acggcatcct
     1621 tcaccgccac ccgaacgccg gagagcagac cctcggctgc gtcggcccga accaggcaat
     1681 agcgaacgat cgcgttgagg ggatcctcgc ccggcgcggg tcggcgtccc gggtcgcgta
     1741 cggcttcgat cggcgccggc agctctggat cctgcgtgtc gaccagctcg atcgtctcga
     1801 tgatcgcctc cacgatgcgc tggatctcga tcctctcgtc gccgtcgagc gtgtaccccg
     1861 atgcgttggc caatgtgtcg agctcgtcgg ccttgatccg atccaccatc cccgtcaacc
     1921 ctcgtcccag aaccgccgcc gcacccagcc ctcctcgatc gggctgatcg tctggcaatc
     1981 gaggagcacc tcggagaact tccagacgcc gtcctccttg cgatagcggt cggcgtagcg
     2041 gccggtgagc aatacggcac gagggccgtc gggggtcacg accgtgcagg gctgccacag
     2101 gtaccaggtg ccgcgggcgg cggcgagatc gtcctgcacc tcgatcatcg gcgcgatcat
     2161 gtagtggaga gcccaagtga tctgcgcgct cacgcccgcg aagaaggcgg cgacggcgtc
     2221 caagccctcg tagcggccga agcgcccgcc gtcccagaca gcgtcgtcgg tgaagaaggg
     2281 ccgcatccgg tccgggtcgt agcccgtgtc gcacacatcc gcgtagcgcg ccttcaggtt
     2341 gcggatctcc tctgtcgcta ggagcttcgc cacctggtgc tcgagcgagt tcatggtcac
     2401 ctcctcggag tcgcgtgctc gtctcgccac gtcactgtcg gagccggctt catcgatcgc
     2461 cacgcgaggg agcaagtcta cgacgcccgc ggcgaccgtc gccatgggcg gagaccacca
     2521 ggtcgggctg cacgatccga ctcgagcatc ggcacattgg acgtggcagt tctcggggtt
     2581 tgcgcagccg cgccgtgcat cgacgcggcc acgtggttga tcgccgttgc ttacgttcgc
     2641 gcggcgggga gcttcaggtc gtagacgaac gtctccgtca gcttcccttt gaggtttgtc
     2701 atcagcgaag agaccgtcag tgatcccttg gccccgaagt acgcacccgt tccccgtttg
     2761 acggggagcg aaaagcttcg gagcatgggc tgcttgaggc ctccgccacc gaaccaatcg
     2821 gcgtggaatc tcttctgggc gagaatcatg ccgccgggaa gcttcgtcgt ctgtgtgacg
     2881 aggaggatcg tctggttctt gtagacgagc cgccagatgc tgtttccgac agccgc
//
LOCUS       DBNGNK010000245         3429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1621353,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000245 DBNGNK010000000
VERSION     DBNGNK010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1621353"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1123
                     /locus_tag="AC150V_07230"
     CDS             275..1123
                     /locus_tag="AC150V_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3762751.1"
                     /translation="MERPDFTWPDGKRLAVYVALCVEHFSYGDGLGLPYSPGLDHPNT
                     YNWAWREYGNRVGGWRLAELFDEYQLPLAILLNTACYDHCPELVAAFRGRGDEIVAHG
                     RTNSEHQNGLDEPDERVLIEEATNAIREHEGIAPAGWMSPGAHPSAHTEDLLAEVGYA
                     YTLDWPMDDQPVWIETRGGPLLTVPYPHEVNDVPMVVLHDGTASAFVDMAIGNLEEML
                     LQSERQPLVYGVTIHTFIVGQPFRIRELRRFLDRLASMRDQVWVTTPGACARHYASLF
                     PAPHPA"
     gene            1120..1929
                     /locus_tag="AC150V_07235"
     CDS             1120..1929
                     /locus_tag="AC150V_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007437984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HIR3762752.1"
                     /translation="MSATRRVAIAGLGTVGLAVAEALDAGIPGCELTAVSARDHGRAQ
                     ERVGGLSRRVPVVPIDDLEQLADLVVESAPAALLPQIAEPFLRTGKTAIVLSSGALLD
                     RGELVDLARRHGGQIVVPTGALLGLDAVAAAAEGEIRSVRMLTRKPVMGLVGAPYLAA
                     RGIEIEGITAPVCVFEGSAREAAVGFPANLNVAVALSLVGIGPDRTRLEVWADPGLER
                     NTHRIEVDADSASFTMSIENVPTENPRTGRITALSVIACLRKLAAPLRVGT"
     gene            1935..2627
                     /locus_tag="AC150V_07240"
     CDS             1935..2627
                     /locus_tag="AC150V_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004021030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR3762753.1"
                     /translation="MDLGHERAHEPTNKSAVRDRFLAVDTSNVADVLDELGLFHQGLA
                     PDFAPYSGTRLSGWAYTIRGQATSFPLGGDADKMQACQGVSSGEVAVWSGGGEGVCYF
                     GELIALGMQENGCVGALVDGGVRDLRWLAQHEFTVFARYRTPVQSIGRWKVNAWQVPV
                     HVRGATSDHVVVNPGDFVLADEDGAIVIPVEHVEAVLVEAERLTAREVAIRQDLTTGV
                     SLAEALAKYGHV"
     gene            2635..>3429
                     /locus_tag="AC150V_07245"
     CDS             2635..>3429
                     /locus_tag="AC150V_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762754.1"
                     /translation="MSGQTIIGALSGKEIRIPDTDVPPMPELMPPGVILDGEPAEGFG
                     EIGGARGYPDVRSFRRKLGLLIPATNTSMEHELWSILYQNGGSRGLDGVGIHTANVTT
                     PRPQLRSEADIREYRRQFLEGLTNAVDQILLAQPQYLIMGMSLEHVLRGIDDVRAPMA
                     EIEDYAGLSMATCHDAISVALARCGARRIGLLTPFDRAGNENAKQMYLDLGFDVVATV
                     GFSCANTLHIAHIPDWAKERAIVELLATPANELDAVVQCGTNMSLSQ"
BASE COUNT          554 a         1188 c         1164 g          523 t
ORIGIN      
        1 gtctggacgg aagcccggcc ggcggccggg acgatcgcat tcgatcacat cgcgggtcgt
       61 aggtgccggt cgccgggcca tctgcgcagg cggcggcatt cgtccggatg agcggccaat
      121 gcgggcatca cggacgagcc gtactcccgc gtgggggtca gggaactcgc ttgtcgcgcc
      181 caccgctccc ggttaacgtg gattcccgct gccgctacac cgctcgatga cgagtcaagt
      241 tcgatctcac aagaggttcc cctacacccc gatcgtggag cggccggatt tcacttggcc
      301 ggacggcaaa cggctcgccg tctatgtcgc gctctgcgtc gagcacttct cctacggcga
      361 tgggctgggg cttccgtact cgccgggact cgaccacccg aacacctaca actgggcgtg
      421 gcgcgagtac ggcaaccgag tcgggggctg gcggctggcc gagctcttcg acgagtacca
      481 gttgccactc gcgatcctgc tcaacaccgc ttgctacgac cactgccccg aactcgtcgc
      541 cgcgttccgc gggcggggcg acgagatcgt cgcgcacggg cgcacgaact ccgagcacca
      601 gaacggcctc gacgaacccg acgagcgcgt gctgatcgag gaagccacga acgcgatccg
      661 cgagcacgaa gggatcgcgc ccgccggatg gatgagcccg ggcgcacacc cgagcgcgca
      721 cacggaggat ctgctcgccg aggtcgggta cgcctacacc ctcgactggc cgatggacga
      781 ccagcccgtc tggatagaga cccgcggggg gccgctgctc accgttccct acccgcacga
      841 ggtcaacgac gtcccgatgg tcgtgctgca cgacggcacg gcgagcgcgt tcgtcgacat
      901 ggcgatcgga aacctcgagg agatgctgct ccagtccgag cgacagccgc ttgtctacgg
      961 cgtcacgatc cacacgttca tcgtggggca gccgttccgc atccgtgagc tccggcgctt
     1021 cctcgaccgc ttggcaagca tgcgcgatca ggtctgggta accacgcccg gcgcctgcgc
     1081 ccgccactac gcttccctct tcccggcgcc ccaccccgcg tgagcgcgac ccgacgtgtc
     1141 gcgattgccg ggctcggcac cgtcgggctt gccgtcgccg aggctctcga cgccggcatt
     1201 ccggggtgtg agctcaccgc agtatccgcg cgcgaccacg gccgtgcgca ggaacgggtc
     1261 ggcggtctgt cgcggcgcgt tccggtcgtg ccgatcgacg acctcgagca gctcgccgat
     1321 ctcgtcgtcg agtccgcgcc ggcggcgctc ctgcctcaaa tcgcggagcc gttcctgcgc
     1381 accggcaaga ctgcgatcgt gctcagctcc ggcgcgctcc tcgaccgcgg cgagctggtc
     1441 gacctcgcac gtcgccacgg aggccagatc gtggtgccga ccggcgcgct gctcgggctc
     1501 gacgccgtag cggcagctgc cgaaggagag atccgatccg ttcggatgct cacgcgcaag
     1561 cccgtgatgg gtctcgtcgg tgcgccgtac ctcgccgcgc gcgggatcga gatcgagggc
     1621 atcaccgcgc ccgtatgcgt tttcgagggg tctgcccgcg aggcggcggt cggattccct
     1681 gccaacctga acgtcgcggt cgcgctgtcg cttgtcggca tcggcccgga tcggacccgg
     1741 ctcgaggtct gggcagaccc cggtctcgaa cggaacaccc accgcatcga ggtcgacgcg
     1801 gactcggcga gcttcacgat gtcgatcgag aacgtcccca cggagaaccc gagaaccggc
     1861 aggatcaccg cgctgtccgt gatcgcctgc cttcgcaagc tggccgctcc gctgcgggtc
     1921 gggacgtagc gcacatggat ctcggacacg aaagagcgca cgaaccgacg aacaaatcgg
     1981 ccgttcgaga cagattcctt gccgtcgaca cctcgaatgt cgccgacgtg ctcgacgagc
     2041 tcgggctctt ccaccagggg ctcgcgcccg acttcgcgcc gtactccgga acgcgcctgt
     2101 cgggctgggc gtacacgatc cggggccagg cgacatcgtt cccgctcggc ggagacgccg
     2161 acaagatgca ggcctgccag ggcgtgtcga gcggcgaggt cgccgtgtgg agcggcggcg
     2221 gcgagggcgt ctgctatttc ggcgagctga tcgcgctcgg gatgcaggag aacggctgcg
     2281 tcggcgcgct cgtcgacggg ggagtccgcg atctgcgctg gctggcgcag cacgagttca
     2341 cggtgttcgc ccgctaccgc acgccggtcc agtcgatcgg cagatggaag gtgaacgcct
     2401 ggcaggtgcc ggtgcacgtg cgcggcgcga cgagcgacca cgtcgtggtc aaccctgggg
     2461 acttcgtgct tgcggacgag gacggtgcga tcgtgattcc cgtcgagcac gtcgaggcgg
     2521 tgctcgtcga ggccgagagg ctgacggcgc gcgaggtcgc gatccggcag gatctcacca
     2581 cgggcgtgtc gcttgctgag gcgctcgcga agtacgggca cgtgtgagcc ggcgatgagc
     2641 ggtcagacga tcatcggtgc gctgtcgggc aaggagatcc gcatcccgga cacggacgtg
     2701 cccccgatgc cagagctcat gccgcccggc gtgatcctgg acggcgagcc ggcggagggc
     2761 ttcggcgaga tcggcggcgc tcgcggctac ccggacgtcc gcagcttccg gcgcaagctc
     2821 ggccttctga tcccggcgac caacacgagc atggagcacg aactctggag catcctctac
     2881 cagaacggcg gatcgcgcgg tctcgacgga gtcggcatcc acaccgcgaa cgtcacgacg
     2941 ccgagaccgc agctgcgaag cgaggcggat atccgcgagt accggcggca gttcctcgaa
     3001 gggctgacca atgccgtcga ccagatcctg ctggcgcagc cccagtacct gatcatgggc
     3061 atgagcctcg agcacgtact gcgcggcatc gacgacgtga gagcgccgat ggccgagatc
     3121 gaggactacg ccggcctttc gatggccacc tgccacgacg ccatctccgt cgcgctcgcg
     3181 cgatgcggtg cgcggcgaat cggccttctc acgccgttcg accgtgccgg caacgagaac
     3241 gcgaagcaga tgtacctcga cctcggattc gacgtcgtcg cgaccgttgg gttctcgtgc
     3301 gcgaacacgc tccacatcgc gcatatcccc gactgggcaa aggagagggc gatcgtcgag
     3361 ctcctcgcca cccccgccaa cgagctcgac gcagtcgtcc agtgcggcac gaacatgagc
     3421 ctctcgcag
//
LOCUS       DBNGNK010000246         3349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_975081,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000246 DBNGNK010000000
VERSION     DBNGNK010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_975081"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..745)
                     /locus_tag="AC150V_07250"
     CDS             complement(116..745)
                     /locus_tag="AC150V_07250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3762755.1"
                     /translation="MKEELRALVREAEGEPAGALVLFHGRGADENDLVPLLDEVDPAR
                     RLLGLAPRGPLSLPPGGAHWYRLGGIPTPDRETFAATFALAATYLDNLPVPLERTVLG
                     GFSQGAVMSLALGLGEGRRRPAAVIALSGFLPRVEDFSLELAALEGYPAFVAHGSLDP
                     VIPVEFGRETASALRDAGADVRWRESRVSHMVDPRVLPELQAFVAEVVP"
     gene            complement(742..1668)
                     /locus_tag="AC150V_07255"
     CDS             complement(742..1668)
                     /locus_tag="AC150V_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3762756.1"
                     /translation="MQFNGIHHVTCITGDAPRNVDFYTRVLGLRLVKKTVNQDDPTVY
                     HLFYADEEGSPGSDITFFEYPGARRGRAGAGMVHTITFRVASADALDFWQARLADESV
                     TVGRSDNRLGFDDPEGLGLELVVSETSDEPLVAVNPEIPAEVALQGFDGVRAFAEAPE
                     ASRNVLEGPLGFSPAGDATWEARGESRGGTYAYDRPPADRGLRGAGTVHHVAWSSRLD
                     EQDVWQRTVADAGLHPTGIIDRFWFRSIYFSEPSGVLFEIATDGPGFAVDEDAEHLGE
                     RLTLPPAFEHLRSQLDDVLTPLPDPRPWATAG"
     gene            1740..2198
                     /locus_tag="AC150V_07260"
     CDS             1740..2198
                     /locus_tag="AC150V_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3762757.1"
                     /translation="MQTSVKQLTAAELGAWRGFLRVHAALIGELDRELAEACGLPLRA
                     YEVLLYLEDAPEGRARMSELADSVLLSQSGLTRLVDRLERDGFVERLPCDDDRRGLYA
                     HLTPAGGAALQEARVTHLVGVRERFLSHFDGGELGELAAFWERLLPGASR"
     gene            2227..>3349
                     /locus_tag="AC150V_07265"
     CDS             2227..>3349
                     /locus_tag="AC150V_07265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762758.1"
                     /translation="MRIGIVVPFSWSFWGAVVEHAELQAAALEERGHYVRLIMANDPP
                     GQFTRVLHPRIGRHGNPPPNVIPVGRSVIVPANGSLPNISLSPRSPFRMHRVLKRERF
                     DVLHLHEPMTPAICVAALVMATCPIVATHHASGMLAWMALGMPSWGWLQSRVDQRIAV
                     SERARASAERWLPGSYEIVPNGVLIPDEADPRGRNHTILFAGRHEPRKGLHVLLHAWP
                     EISRRTGARLRIAGADPLAVRLLLSRVHVADDDIDVLGFLSQDELTIEMSSTKALVAP
                     SLGGESFGMVLTRAFACATPVVASDIVGYREVITPDTAVSMPPEDPAALADAVCSLLA
                     DEPRRARMGAAARELAVAAYGWPDIAGKLEEIYERVLGRD"
BASE COUNT          488 a         1154 c         1183 g          524 t
ORIGIN      
        1 gccagatagg tcgatcccca gacgacgtac acgacggcga gcgccgtcca cagcttccaa
       61 gctcgtggcc ccatctagcg agtgaaacgc gcctcgctgt ctcccatgtg aggcgctacg
      121 gaaccacctc ggcgacgaac gcctgcagct cgggcagcac gcgcggatcg accatgtgcg
      181 acacccgcga ctcgcgccag cgcacgtcgg cgcccgcatc gcgcagtgcg gaagccgtct
      241 cgcgaccgaa ctcgaccggg atcaccgggt cgagcgagcc gtgggcgacg aaggccggat
      301 atccctcgag cgccgcgagc tcgagcgaga agtcctcgac tcggggaagg aagcccgaga
      361 gggcgatgac cgccgccggc cggcgccgtc cctcgccgag gccgagcgcc aggctcatca
      421 cggcgccctg cgagaagcct ccgagcaccg tcctctcgag tgggacgggg aggttgtcga
      481 gataggtcgc ggcgagcgcg aaggtcgcgg cgaaggtctc tcggtcgggg gtcgggatgc
      541 cgccgagccg ataccagtgc gctcctcccg gcggcagaga gagcggaccc cgcggagcca
      601 gcccgagcag gcgtcgggcg ggatcgacct cgtccagcag cgggacgagg tcgttctcgt
      661 ccgcgccgcg gccgtggaag aggacgagcg cgcccgccgg ttctccttcg gcctcgcgga
      721 cgagtgcccg cagctcctct ttcacccggc ggtcgcccag ggccgggggt cgggcagcgg
      781 cgtgagcacg tcgtccagct ggctgcggag gtgctcgaag gcgggcggca gcgtcagccg
      841 ctccccgagg tgctctgcgt cctcgtccac tgcgaagccg ggcccgtccg ttgcgatctc
      901 gaacaggacg ccgctcggct cgctgaagta gatcgatcgg aaccaaaagc ggtcgatgat
      961 tccggtcgga tgcaggccag cgtcagcaac cgtccgctgc cagacgtcct gctcgtcgag
     1021 acgcgacgac caagcgacat ggtggacggt gccggcgccg cgcagacctc gatcggcggg
     1081 agggcgatcg tacgcgtagg tgccgccgcg gctctcgccg cgcgcctccc acgtcgcgtc
     1141 gcccgctgga ctgaagccga gtggtccctc gagcacgttc cggctcgcct ccggcgcttc
     1201 tgcgaacgcg cgaacgccgt cgaagccctg cagcgccact tcggccggga tctccgggtt
     1261 gacggcgacg agcggctcgt cggaggtttc cgacacgacg agctcgagcc cgaggccctc
     1321 cgggtcgtcg aagccgaggc ggttgtccga gcgtcctacg gtcacgctct cgtccgcgag
     1381 tcgcgcctgc cagaagtcga gcgcgtcggc agatgcgacg cggaaggtga tcgtgtgcac
     1441 catgcctgcc ccggcgcggc cgcgacgtgc tcccgggtac tcgaagaacg tgatgtcgct
     1501 gcccgggctg ccttcctcgt cggcgtagaa aagatggtag acggtgggat cgtcctggtt
     1561 gaccgtcttc ttcacgagcc ggagcccgag cacacgggtg tagaagtcga cgttccgggg
     1621 cgcgtctccc gtgatgcacg tcacatgatg gatgccgttg aactgcatga tcacctctca
     1681 ggcgcccagc cgggcgtcgt tgtagcgtgg agaactcgca ccaggcagaa tagttgtaga
     1741 tgcaaacaag cgtcaagcag ctcacggccg ccgagctcgg ggcctggcgg ggcttcctcc
     1801 gcgtccatgc cgctctgatc ggcgagctcg accgcgagct cgccgaggca tgcgggctgc
     1861 cgctgcgcgc ctacgaggtg ctgctctacc tcgaggacgc tccggagggc cgggcacgga
     1921 tgtccgagct cgccgactcg gtgttgctga gtcagagcgg gctgacccgg ctcgtcgatc
     1981 ggctcgaacg agacggattc gtcgagcggc tgccctgcga cgacgaccgg cgcgggctct
     2041 atgcgcatct cacccccgcg gggggcgcgg cgctccagga ggcccgggta actcacctcg
     2101 taggcgtccg ggagcgcttc ctctcccatt tcgacggcgg ggagctgggt gagctagccg
     2161 cattttggga gcggctcctg ccgggggcgt cccgttagcg acgagacgat cctttaggct
     2221 ggcgtcgtgc ggatcggcat cgtcgtgccc ttctcatggt cgttctgggg cgcggtcgtc
     2281 gagcacgcgg agcttcaggc tgcggcgctc gaggagcgcg gccactacgt gcgcttgatc
     2341 atggccaacg atccgcccgg ccagttcaca cgggtgctcc acccgcgtat cggccgccac
     2401 ggcaatccgc cgccgaacgt gattccggtc ggacggtcgg tgatcgtgcc ggcgaacggc
     2461 tcgctgccga acatctcgct gagcccccgc tcgcccttcc ggatgcaccg ggtgctgaaa
     2521 cgtgagcgat tcgatgtgct gcacctgcac gagccgatga cccccgcgat ctgtgtcgcc
     2581 gcgctcgtga tggcgacgtg cccgatcgtc gccacccacc acgcgtcggg gatgctggcc
     2641 tggatggcgc tcgggatgcc gtcgtggggc tggttgcagt cgcgtgtcga ccagcgaatc
     2701 gcagtctccg agcgggcccg cgcatcggcc gagcgctggc ttccggggtc gtacgagatc
     2761 gtcccgaacg gtgtactgat ccccgacgag gccgacccgc gcgggaggaa ccacacgatc
     2821 ttgttcgccg gtcgtcatga gccgcggaag gggctgcacg tcctgttgca tgcctggccc
     2881 gagatcagcc gccgcaccgg cgcgaggctt cgtatcgcgg gcgctgatcc gctggccgtc
     2941 cggctgctcc tttcccgcgt ccacgtggcc gatgacgaca tcgacgtcct cggcttcctt
     3001 tcgcaggacg agctcacgat cgagatgtcg tcgacgaagg cgcttgtcgc gccgtcgctc
     3061 ggcggagaga gcttcggcat ggtcttgacg cgggcgttcg cctgcgcgac acccgtcgtc
     3121 gcctcggaca tcgtcggcta ccgggaggtc atcacgcccg acacggcggt gtcgatgccc
     3181 cccgaggacc ccgcggcctt ggccgacgcg gtgtgctcgc tcctcgccga cgagccgcgc
     3241 cgggcgcgga tgggcgcagc cgcgcgggag ctcgccgtgg ccgcatacgg ctggccggac
     3301 atcgccggca agctcgagga gatctacgag cgcgttctcg gtcgggatc
//
LOCUS       DBNGNK010000247         2758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1764341,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000247 DBNGNK010000000
VERSION     DBNGNK010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1764341"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="AC150V_07270"
     CDS             <1..844
                     /locus_tag="AC150V_07270"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923501.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR3762759.1"
                     /translation="EAAPIKWMAPFAGAAMGEHFLYNGTHSLCIYDDLTKHADAYRQL
                     SLLLRRPPGREAFPGDVFYLHSRLLERACKLSDELGAGSLTALPVIETQAGDVSAYIP
                     TNVISITDGQIFLESDLFYSGVRPAINVGISVSRVGGNAQTKAMKKVAGRLRLDLAQY
                     RELEAFAQFGSELDAATQQALARGERMVATLNQPQYQPWPLEEQVVAIYAGINGHLDP
                     VPVPQVPRYQEELRGFLRTEASVYAAIRDSGDLSDETTAALDEQLQKFGHGFNIEEEK
                     GLVA"
     gene            856..1746
                     /gene="atpG"
                     /locus_tag="AC150V_07275"
     CDS             856..1746
                     /gene="atpG"
                     /locus_tag="AC150V_07275"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR3762760.1"
                     /translation="MASTQDLKRRIRSVRNTRKITRAMELVAAAKLRRAEQRIEAMRP
                     YAETMRELIAGVGRASSSVRGLPLLQQRDQVSSVLIVPLTGDRGLAGAFNAQVLRRAF
                     ALERELQAEGLNVRWLGVGKKGRSTLSFRKRELLGDFIGFTDRPAYSDAQGIAHRVAE
                     LFVGGDIDRVVLVYNTFISALTQQVTEQELLPIAAEVLQTDEEERRDDTLRGDFIFEP
                     EPEEILARLLPVYVETQIFRALLESTASEQGARMTAMRNASKNAGELIDRLTLQMNRA
                     RQAEITQEILEVVAGAEALA"
     gene            1822..>2758
                     /locus_tag="AC150V_07280"
     CDS             1822..>2758
                     /locus_tag="AC150V_07280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3762761.1"
                     /translation="MATDPRSLEPVTAGADGGAVAVDAAGQVDVKARGYWEQVWIRFR
                     RDRMAVFSIFFIVLLVLASFPGAWIAEQLLGHGPNTIFFDGVDDQLVPVGPWSYVTNP
                     ESGDRQLLVLGADGSLGRDEFLRLLYGGRVSLEVACLSTIGVMIVGVLLGAIAGYYRG
                     WIDTSVSRVIEVTMAFPALLFIIALASTVGGRLDGVTLGIFPPGVFTLVLVFTVFGWF
                     YPARIIRAQVLSLREKEFVEAARMIGASDARIIRSHVLPHLVAPIIVFSTLIIAGYVL
                     GEAGLSVLNLGVKLPTASWGNLLASAPEYYTAQPFL"
BASE COUNT          421 a          957 c          914 g          466 t
ORIGIN      
        1 ggaggccgct ccgatcaaat ggatggcgcc gttcgccggt gccgcaatgg gcgagcactt
       61 tctctacaac ggcacgcact ccctctgcat ctacgacgac ctgacgaagc acgcggacgc
      121 gtaccgacag ctgtcgctgc tactgcggcg cccgccgggt cgcgaggcgt tcccaggaga
      181 cgtcttctat ctgcactccc gtctgctgga acgtgcctgc aagctgtcgg acgagctcgg
      241 tgccggctcg ctcacggcac tgccggtgat cgagacgcag gccggtgacg tgtcggccta
      301 catcccgaca aacgtcatct cgatcacgga cgggcagatc ttcctcgagt cggacctctt
      361 ctactcgggc gtccggcccg cgatcaacgt gggtatctcc gtctcgcggg tgggcgggaa
      421 cgcacagacg aaggcgatga agaaggtcgc cggacggctg aggctcgacc tggcgcagta
      481 ccgcgagctt gaggcgttcg cccagttcgg ctcggagctc gacgcggcga cgcagcaggc
      541 tctcgcgcgc ggggagcgga tggtcgcgac gctcaaccag ccgcagtacc agccgtggcc
      601 gctggaggag caggtggtgg ccatctacgc agggatcaac gggcatctcg atccggttcc
      661 ggttccgcag gtgccgcgct accaggagga gctgcgcggc ttcctgcgca ccgaggcctc
      721 cgtctacgca gcgatccgcg acagcggtga cctctccgac gagaccaccg ccgcgctcga
      781 cgagcagctg cagaagttcg gccacggctt caacatcgaa gaggagaagg ggctcgtcgc
      841 ctgacgcgaa cctagatggc atcgacccaa gacctcaaac gccggatccg ctccgtccgc
      901 aatacgcgga agatcacgcg tgccatggag ctcgtggcgg cagcgaagct gcgtcgcgcg
      961 gaacagcgga tcgaggcgat gcgtccgtac gcagagacga tgcgagagct gatcgcgggc
     1021 gtcggccggg catcctcgtc ggtgcgcggg ctgccgctgc tccagcagcg agaccaggtc
     1081 tccagtgtgt tgatcgttcc gctcaccggc gatcgcggcc tcgccggcgc gttcaacgcc
     1141 caggtactgc gtcgcgcctt cgccctcgag cgggagctcc aggccgaggg tctgaacgtg
     1201 cgctggctcg gcgtcggcaa aaagggccgc tcgacgctct ccttccgcaa gcgagagctg
     1261 ctcggcgact tcatcggctt caccgaccgg ccggcctact cggacgcgca ggggatcgcc
     1321 caccgcgtcg ctgagctctt cgtcggcggc gacatcgatc gggtcgtgct cgtctacaac
     1381 acgttcatct cggcgctcac gcagcaggtg accgagcagg agctcttgcc gatcgcggcg
     1441 gaggtgctcc agaccgacga ggaggagcgg cgggacgaca cgctccgcgg cgacttcatc
     1501 ttcgagccgg agcccgagga gatcctcgcc cggctgctcc ccgtgtacgt cgagacgcag
     1561 atcttccggg ccttgctcga gtcgaccgcc tccgagcagg gggcccgcat gaccgcgatg
     1621 cgcaacgcct ccaagaacgc cggcgagctg atcgaccggc tcacgctgca gatgaaccgc
     1681 gcgcgacagg ccgagatcac gcaggagatt ctcgaagtcg tggccggcgc ggaagcgctt
     1741 gcgtagcggc taaccaaacc cgccggcgcc tcgtctgagt ctctatcgac gcgccgtgcc
     1801 cggcgctatg ctccctgacc gatggcgacc gacccgcgca gtctcgagcc cgtaaccgcc
     1861 ggggcggacg ggggggcggt cgcggtcgat gccgccggtc aggtggacgt caaggcccgc
     1921 ggctactggg agcaggtgtg gatccgtttc cggcgtgacc gcatggcggt cttctcgatc
     1981 ttcttcatcg tcctcctcgt gctcgcgtcg ttcccgggtg cctggatagc cgagcagctg
     2041 ctcgggcacg gcccgaacac gatcttcttc gacggcgtcg acgaccagct cgtcccggtc
     2101 ggcccgtggt cgtacgtcac caacccggag tcgggcgacc gccagctgct cgtactcggc
     2161 gccgacggct cgctcggccg cgacgagttc ctgcggctcc tctacggcgg gcgtgtgtcg
     2221 ctcgaggtcg cctgcctttc gacgatcggc gtgatgatcg tcggggtgct gctcggcgcg
     2281 atcgccggct actaccgcgg ctggatcgac accagcgtct cacgcgtcat cgaggtgacg
     2341 atggctttcc cggcgctgct cttcatcatc gccctggcat cgacggtcgg cgggcggctc
     2401 gacggggtca cgctcggcat cttcccgccc ggggtattca cactcgtact cgtcttcacg
     2461 gtcttcgggt ggttctaccc ggcccgcatc atccgggcgc aggtgctctc gctgcgtgag
     2521 aaggagttcg tcgaggctgc gcgcatgatc ggagcctctg acgcgcgcat catccgctcg
     2581 cacgtcctgc cgcacctcgt cgcgccgatc atcgtcttct cgacgctcat catcgccggc
     2641 tacgtgctcg gcgaagccgg cctctccgtc ctcaatctcg gcgtgaagct tccaaccgcg
     2701 agttggggca acctgctggc ctcggcgccc gagtactaca cggcgcagcc gttcctca
//
LOCUS       DBNGNK010000248         8269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_975196,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000248 DBNGNK010000000
VERSION     DBNGNK010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8269
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_975196"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="AC150V_07285"
     CDS             complement(<1..674)
                     /locus_tag="AC150V_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762762.1"
                     /translation="MSRIPIRLRLTLVFALAMAVVLAALGAFLYVRLERTLEEQVDDG
                     LRARAATTSALVRELGGEGVGGALGVGGDEHFTQILAEDGSVLAASPSSFTEPAASLD
                     ETREAGSGAVFLDRDFVPLFDDNSGRLLVTPVDDEAGRVTLVVGASLEDAEDSLAGLL
                     TALLIVGPMALVVASLAGYGVAAAALRPVDAMRKKAMTISAKTAAGRLPLPDARDEVY
                     RLGTTL"
     gene            complement(671..1348)
                     /locus_tag="AC150V_07290"
     CDS             complement(671..1348)
                     /locus_tag="AC150V_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3762763.1"
                     /translation="MRILVVEDEVKMAGLLRRGLTEEGHATDVAATGDDALWMAQAVE
                     YDAIVLDLMLPGIDGFEVCRRLRQDGVWSPVLILTARDAVEDRVAGLDAGADDYLPKP
                     FSLAELFARLRALARRGKVERPIVLEVGDLRLNPTTRDVRRGDVEIALSAKEFALLEA
                     FMRRPGQVLSRYQLLEHAWDYAYENRSNIVDVYIRYLRDKIDRPFERDSLETVRGVGY
                     RLRAPDR"
     gene            1478..1840
                     /locus_tag="AC150V_07295"
     CDS             1478..1840
                     /locus_tag="AC150V_07295"
                     /inference="COORDINATES: protein motif:HMM:NF051432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR3762764.1"
                     /translation="MTRRLVLVGVLVLALAALGTGIAFAAGGVGSGGNVSGPSADRAG
                     DAARASTGGGEILEVEEGDDPGSAYEVEIRTADGMVAEVLLDGDFNVIDTVAGDDDGN
                     ENEQNETDAKDGENEQSG"
     gene            complement(1837..2784)
                     /locus_tag="AC150V_07300"
     CDS             complement(1837..2784)
                     /locus_tag="AC150V_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3762765.1"
                     /translation="MIPGYIALARSGELDRRAAEAFELLGPRCVVCPRGCKVDRRADE
                     VGLCAIGRYAVVASHFAHFGEEDCLRGRRGSGTIFFSGCNLRCVFCQNHDISWQVRGE
                     RVEAARLAEMMLELQDAGCHNINWVTPEHVVPQILEALPHAVARGLSLPLVYNTSSYD
                     GLDSLRLLDGVVDVYMPDLKLLTREGSRRYLKRSDYHEIARDAVREMYRQVGDLVVDE
                     KGLARRGVLLRHLVMPGQLAETEAILVFIAEELGQECYVNLMAQYHPAGRVGRGGEYE
                     EIDRRLHPDEFERALALAESLGLRLDARSAAERHRLMRI"
     gene            2822..3793
                     /locus_tag="AC150V_07305"
     CDS             2822..3793
                     /locus_tag="AC150V_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR3762766.1"
                     /translation="MRLLTALADTYNPYGFVDELVAKELEAESGWTVIGTQAVTADTL
                     ADAEVLLAMMVPVTAEALDGSTRLRLIQAASRGYDGIAVDAAARLGIPVCNVSTPGHA
                     STVAEHTFALLLALAKRIVEGDRAIAAGDWPTGDLIARGLTELRGKTLGIVGLGEIGK
                     EVAKRAHAFGMKLVYADEIRSVELEDRYGMARVDLASLLRTADVVTVHVPLTEDTDGL
                     IGESEIESMRSGAILLNTSRGPVVDVDAVAAALSAGRLQAGLDVFEPEPPPARHAILS
                     APNVVRSPHVGGVTAEALRRVVADALANIRRLERGEPLRNIVNGVST"
     gene            3790..4098
                     /locus_tag="AC150V_07310"
     CDS             3790..4098
                     /locus_tag="AC150V_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIR3762767.1"
                     /translation="MIKRITLVKRRDGMTPEDFARHWTTTHAELAKRLPGIRGCRINV
                     VREWIDGDQPWDGIGEIWFDTEETMDAAFAALADEFAADRPLFIGDACRALVDEFVVV
                     "
     gene            4232..4684
                     /locus_tag="AC150V_07315"
     CDS             4232..4684
                     /locus_tag="AC150V_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762768.1"
                     /translation="MVERAAVDPGSQAGPGGVRRILERFRRPSGVPTAAADDVAAELA
                     PLFGLLDEIESEAAAIRAATERRMRAERVEAAEEVEAILTEATHQAESERGEVAKAAR
                     RAAAAEAHALVEEGEREAARVRKNARGQMPSLVDEVIENLSSLGEQPR"
     gene            4681..5607
                     /locus_tag="AC150V_07320"
     CDS             4681..5607
                     /locus_tag="AC150V_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762769.1"
                     /translation="MSAGWIAGSVRARLLLGRRLGPGAARELAAAPSLDAALSRLAGT
                     AYARAAEPGPTLEAVQHQVATVTLVHLRLLAGWLPRDAAELLRPLAGWFELANIEDRL
                     AYLQGAALRPAFELGSLAACWPQASLAQSADELWDVLAGSAWGYGGGADVRELRAGLR
                     VALAARVVAAVPEARSWTAGAVALMLARSALEGSRELGLRPGDHLGGLPWRVPHTMSL
                     DGIRAGLPAEAAWSLEGIEAAEELWRAEAAWWQRVEADATTLARGPRAGRSVILGVVA
                     LLALDAVRVSAALGVATRSGGKAAKEAFDALV"
     gene            5594..7000
                     /locus_tag="AC150V_07325"
     CDS             5594..7000
                     /locus_tag="AC150V_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HIR3762770.1"
                     /translation="MPSSSRALPVRMSRIALVTPKDRLRDAIVALAEAGSVELVGSLP
                     AAEGAEAEALHRFEHDHPRSRADEPCVLREAPDLAALEERGACELLAGEVEVRRRAAM
                     AVEHGTFVALLGWTPTDGLPALNERLADAGATAVEMPAPAWAEPPTQLTPAAAARPFR
                     PLVTTYGVARYADVDPTLFAGFAFVLMFGIMFGDVGHGLVLALLGLLLSRVRRGRLKQ
                     IQPMWPLVAAAGVSAAFFGLLYGEAFGPTGIVPRLWLDPLEDPIPLLVLALVVGLGLL
                     AVSHAFGVVNRYREAGAIEAILSPSGIAGLFILLGGAAAIAGAFTDATTLAYVGAVVA
                     VLGVALLGVGFVISAGGGASGVAQAGIELVNSVIRIGANAVSFARLAAFGILHAALGL
                     VVFAAVGALWGGVAGSIAAVLVFVIGNLAAFSVGLLVTGVQALRLEFYEIFSRIFAGE
                     GHPFRPWALPVVQPKEES"
     gene            7000..7416
                     /locus_tag="AC150V_07330"
     CDS             7000..7416
                     /locus_tag="AC150V_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR3762771.1"
                     /translation="MLSLLVALPVIVLAAVVTVTGIRRAPERGFRRLVVINGALALGA
                     LGLLAAILVGIVEPASAAESTSSSDPRAALIGASIATAGSALGAGIAVSYTGSAALAA
                     ISEKPEMFGRAMVIVGLAEGIAIYGLIVSILLLSYA"
     gene            7413..7631
                     /locus_tag="AC150V_07335"
     CDS             7413..7631
                     /locus_tag="AC150V_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009322817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR3762772.1"
                     /translation="MSRVVAIGEEARIAGYALAGATAVSARDAAEAQAAWETLPPDTA
                     LVILTRAAHEALEDVVGERRLLWVVLPE"
     gene            7628..8128
                     /locus_tag="AC150V_07340"
     CDS             7628..8128
                     /locus_tag="AC150V_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762773.1"
                     /translation="MNLEALRGSFLAAVSEDAERAVSECLRECRARIAEAQRQADDVV
                     ARARSAGESDSEGEVGHILVSARRQAQARVLAARREVYEDFRAEALRAALALRGTSQY
                     DALLARLEDDVRRRLGEDAQLEIDPHDRGGVFGRSGSRRLDYSLAALVDDCVSELGER
                     VERLWT"
     gene            8125..>8269
                     /locus_tag="AC150V_07345"
     CDS             8125..>8269
                     /locus_tag="AC150V_07345"
                     /inference="COORDINATES: protein motif:HMM:NF014873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762774.1"
                     /translation="MTDGAVRRVNGPVVDVDGIDVAMLDLVEVGASRLPGEVIGLSGP
                     VATV"
BASE COUNT         1234 a         2872 c         2909 g         1254 t
ORIGIN      
        1 ttcagcgtcg tgcccagccg gtagacctcg tcccttgcgt cgggtagagg caggcgcccc
       61 gcggcggtct tggccgagat cgtcatcgcc ttcttccgca ttgcgtccac ggggcgcagt
      121 gccgccgccg caacgccgta cccggcgagc gacgccacga cgagcgccat cggaccgacg
      181 atgagaaggg ccgtcagcag tcccgccagc gagtcctcgg catcctcgag cgacgcaccg
      241 acgacgaggg tcacgcggcc ggcttcgtcg tcgaccggcg tgacgagcag ccgcccggag
      301 ttgtcgtcga acagcggcac gaagtcgcgg tcgaggaaaa cggcgcccga tcccgcttct
      361 cgcgtctcgt cgagcgacgc agccggctcc gtgaagctgc tgggcgacgc agccagcacc
      421 gagccgtcct ccgccaggat ctgggtgaag tgctcgtctc ctccgactcc gagtgcgccg
      481 ccgacccctt cgccgccgag ttcacggacg agcgccgacg tggtcgcggc acgcgcccgc
      541 agaccgtcgt cgacttgctc ctccaacgtt ctctcgagcc gtacgtagag gaaggccccg
      601 agagcggcga gcacgaccgc catcgccaga gcgaagacga gcgtcaatct cagcctgatc
      661 gggatcctgc tcatcggtcg ggcgcccgca atcggtagcc gacgccgcga acggtctcca
      721 gcgagtcacg ctcgaacggc ctgtcgatct tgtcgcgcag gtaccggatg tagacgtcca
      781 cgatgttcga gcggttctcg tacgcgtagt cccatgcgtg ctcgagcagc tggtagcggg
      841 agagcacctg tccgggccgg cgcatgaacg cctcgagcag tgcgaactct ttcgcggaca
      901 gcgcgatttc gacgtcgccg cgccggacgt cgcgcgtcgt agggttcagg cgaagatcgc
      961 ccacttcgag gacgatcggg cgctcgacct tgccgcggcg cgcgagcgcc cgcaaccggg
     1021 cgaagagctc ggcgagcgag aacggcttcg gcaggtagtc gtccgctcct gcgtccaaac
     1081 ccgccacccg gtcctcgacg gcgtcccgcg ccgtcagtat cagcaccggt gaccagaccc
     1141 cgtcctgacg cagacgccgg cacacctcga agccgtcgat gccggggagc atgagatcga
     1201 gcacgatcgc gtcgtactcg accgcctggg ccatccagag ggcgtcgtcg cccgtggcgg
     1261 cgacgtctgt cgcatgtccc tcctcggtca gcccgcgccg gagcaggccg gccatcttca
     1321 cctcgtcctc gacgaccagt atgcgcatcg tcctgattgt cgccggattc gatgagagct
     1381 cgatgagagc gacgggtctc gcggctttca cggctgtctc atctggcctc cctaccgtga
     1441 tcgccgacct gaagaccagt cgaccgagga ggtccacatg acacgaagac tcgtactcgt
     1501 cggtgtgctc gtgctcgcgc tcgcggcgct cggcaccgga atcgccttcg cggcgggcgg
     1561 cgtcgggagc ggcggcaacg tctccgggcc ctcagccgat cgcgcgggcg acgccgcacg
     1621 cgcgtccacg ggcggcggcg agatcctcga ggtggaggaa ggagacgatc ccggctcggc
     1681 ctacgaggtc gagatccgca ctgccgacgg catggtggcg gaggttctgc tcgacggcga
     1741 cttcaacgtc atcgacaccg tcgccggcga cgacgacggc aacgagaacg agcagaacga
     1801 gaccgacgcc aaggacggcg agaacgagca gtccggctag atccgcatga gccgatgccg
     1861 ctcggcggcg ctacgcgcgt ccagccggag gcccaggctc tcggcaagcg ccagcgcccg
     1921 ctcgaactcg tctgggtgga ggcggcggtc gatctcctcg tactcgccgc ctctgccgac
     1981 cctccccgca gggtggtact gggccatcag gttcacgtag cattcctgtc cgagctcctc
     2041 ggcgatgaag acgaggatcg cctctgtctc cgccagctgc ccgggcatca cgagatgccg
     2101 cagcagcacg ccacgacggg cgagcccctt ctcgtcgacg acgaggtcac cgacctgccg
     2161 atacatctcg cggaccgcgt cgcgggcgat ctcgtggtag tcggagcgct tgaggtagcg
     2221 tcgcgagccc tctcgcgtca gcagcttgag gtctggcatg tacacgtcga cgacgccgtc
     2281 gagcagccgc aggctgtcga ggccgtcgta cgagctcgtg ttgtagacga gcggtaggga
     2341 aaggccgcgc gcgacggcat gcgggagggc ctccaggatc tgcgggacca cgtgctccgg
     2401 cgtgacccaa ttgatgttgt ggcacccggc gtcctgcagc tcgagcatca tctccgcgag
     2461 tcgagccgct tccacgcgct caccgcggac ctgccaggag atgtcgtggt tctggcagaa
     2521 gacgcagcgc aggttgcagc cgctgaagaa gatcgttccc gagccgcgcc ggccgcgcag
     2581 gcagtcctcc tcgccgaagt gggcgaaatg ggaggcaacg accgcgtacc gcccgatcgc
     2641 gcagagtccg acctcgtcgg cgcggcggtc gaccttgcaa ccccgcgggc agacgacgca
     2701 gcgcggcccg agcagctcga acgcctcggc ggctcgacga tcgagctcgc ccgagcgggc
     2761 cagcgcgatg tagcccggga tcactgcgct cgacgatagc gagttagagt cgcgagcagg
     2821 gatgcgactg ctgaccgcgc tggccgacac gtacaacccc tacggcttcg tggacgagct
     2881 cgtcgccaag gagctcgaag cggaaagcgg ctggaccgtc atcgggacgc aggccgtgac
     2941 cgccgacacg ctcgccgacg ctgaggttct gctcgcgatg atggttcccg tcaccgcaga
     3001 ggcgctcgac ggatcgaccc ggctccgact gatccaggcc gccagccgcg gatacgacgg
     3061 catcgcggtc gacgcagcgg cgcggctcgg catcccggtc tgcaacgtgt ccactccggg
     3121 acacgcgagc accgtcgcag agcacacgtt cgccctcctc cttgcgctcg ccaagcgcat
     3181 cgtcgagggc gaccgggcca tcgcggccgg ggactggccg accggtgacc tcatcgcgcg
     3241 aggcctgacc gagcttcgcg gcaagacgct cggaatcgtc ggcctcggcg agatcggcaa
     3301 ggaggttgcg aagcgcgcgc acgccttcgg catgaagctg gtctatgcgg acgagatccg
     3361 gtcggtcgag ctcgaggatc gctacgggat ggcacgcgtc gatttggcga gcctcctccg
     3421 cacggccgac gtggtgaccg tccacgtccc gctgaccgaa gacaccgacg gactgatcgg
     3481 agagagcgag atcgagtcga tgagatccgg cgcgatcctg ctcaacacgt ctcgcggacc
     3541 ggtcgtcgac gtcgacgcag tggccgcagc gctgagcgcg ggccgcctgc aggcgggcct
     3601 cgacgtcttc gagcccgagc caccgccggc gcgccacgcg attctctctg ccccgaacgt
     3661 cgtgcggtcg ccgcacgtcg gcggcgtcac ggcagaggcg ttgcggcggg tggtggcgga
     3721 cgcactggcc aatatccggc gactggagcg cggtgagccg ctccgcaaca tcgtgaacgg
     3781 agtcagcaca tgatcaaacg gatcacactc gtcaagcgac gcgacggcat gacgccggag
     3841 gacttcgccc gccactggac caccacccac gccgaactcg cgaagcgtct gcccggcatt
     3901 cgcggctgcc gtatcaacgt cgtccgggaa tggatcgacg gcgaccagcc ttgggacggc
     3961 atcggagaga tctggttcga cacggaggag acgatggacg cggcgttcgc ggcgctggca
     4021 gacgagttcg cagccgaccg ccctctcttc atcggcgacg catgcagggc gctcgtcgac
     4081 gagttcgtcg tcgtctagag acaccgaaga gcgccggaaa acgcgctccg aggctgcgga
     4141 cagtccacag ggcaattgcc gaaggctgcc gatgcggccc cgggcggtgc ctgggaccct
     4201 cagactgcaa ccgcagcaga ggacgtgggc gatggtcgag cgcgccgcag tagatcccgg
     4261 tagtcaggct gggcccggag gggtacgccg gattctggag cggttccggc gcccgtccgg
     4321 cgttcccact gcggccgccg acgacgtggc cgccgagctg gcgcccttgt tcgggcttct
     4381 cgacgagatc gagtccgagg cggccgccat acgggccgcc accgagcggc gcatgcgagc
     4441 cgagcgggtg gaggccgccg aggaggtcga agcgattctg accgaggcga cgcaccaggc
     4501 ggagtcggag cgcggcgagg tcgccaaggc agcgcgccgc gccgcggctg ccgaagcgca
     4561 tgccctcgtc gaggaaggcg agcgcgaggc agcgcgcgtc cggaagaacg cccgcggcca
     4621 aatgccatcg ctcgtcgacg aagtcataga aaacctctcg agcctggggg agcagccccg
     4681 atgagcgccg gctggatcgc aggaagcgtg cgagcacggc tgctgctcgg acgcaggctg
     4741 ggcccgggcg ccgcccgcga attggccgcc gcaccgtcgc tcgacgctgc gctgtcgcga
     4801 cttgccggca ccgcgtacgc gcgggccgcg gagccgggac cgacgctcga ggccgttcag
     4861 catcaggtcg cgaccgtcac tctcgtccat cttcgtctgc tcgcaggctg gctcccgcgt
     4921 gacgcggctg agctgctccg gccactcgcg gggtggttcg agctggcgaa catcgaggac
     4981 cggcttgcgt acctgcaggg cgccgcactg agaccggcgt tcgagctcgg cagcctcgcc
     5041 gcctgttggc cgcaggcctc tctcgcgcag agcgccgacg agctatggga cgttcttgcc
     5101 gggtctgcct ggggctacgg cggtggtgct gatgtgcgcg agcttcgagc gggtctccgt
     5161 gtcgccctgg cggcgcgggt ggtcgcggcg gtgccggaag cccgatcctg gacggccgga
     5221 gcagtcgcgc tcatgcttgc cagatccgct ctggaaggga gtcgtgaatt gggactgcgt
     5281 ccgggcgacc atctcggcgg actcccgtgg cgcgttccgc acacgatgag cctcgacggg
     5341 atccgagcgg gcctcccggc cgaggcggcc tggtcgctcg aaggcatcga agcggccgaa
     5401 gagctgtggc gcgccgaggc agcctggtgg cagcgcgtcg aagccgacgc gacgacgttg
     5461 gcgcggggcc cgcgcgccgg acgaagcgtc atcctcggag tggtcgcgct gctcgcactc
     5521 gacgccgtcc gggtcagcgc ggcgctcgga gtcgccacca gaagtggcgg gaaggcggca
     5581 aaggaggcgt tcgatgccct cgtctagccg cgctctgccg gttcggatga gccggatcgc
     5641 gcttgtcacg ccgaaggatc ggctacgcga cgccatcgtc gccctcgccg aggccggctc
     5701 ggtcgagctc gtcggctcgc tgcccgccgc ggaaggcgcg gaggccgagg ccctccaccg
     5761 cttcgagcac gaccatccca ggtcccgggc cgacgagccg tgcgtccttc gagaggcgcc
     5821 tgacctcgct gccttggagg agcgaggcgc atgcgagttg ttggccggcg aggtcgaggt
     5881 gcggcgcaga gcagcgatgg cggtcgagca cggaacgttc gtcgcactgc tcggctggac
     5941 gcccaccgac ggactcccgg cgctcaacga gcgccttgcg gacgcaggtg cgacggccgt
     6001 cgagatgccg gcaccggcct gggccgaacc gccgacgcag ctcacgccgg ccgcggccgc
     6061 acgaccattc aggcctctgg tcacgacgta cggcgtcgcg cgctacgcgg acgtcgaccc
     6121 cacgctcttc gccggcttcg ccttcgtcct catgttcggg atcatgttcg gagacgtcgg
     6181 ccacgggctc gtgctcgcgc tgctgggcct gcttctgagc cgcgttcgtc gcggccgcct
     6241 gaagcagatc cagccgatgt ggcccctcgt ggccgcggcc ggggtctccg ccgccttctt
     6301 cggcctgctg tacggcgagg cattcggccc cacgggcatt gtcccgcggc tctggctcga
     6361 tccgctggag gatccgattc cgctgctcgt cctggccctc gtcgtcgggc tcgggctgct
     6421 ggccgtgagc cacgcgttcg gcgtcgtcaa ccgctaccgg gaggcgggtg cgatcgaggc
     6481 gatcctgtcg ccgtccggaa tcgccggcct cttcatcctg ctcggcgggg ctgccgccat
     6541 cgcgggcgcg ttcacggacg ccacgacact cgcctacgtc ggcgccgtgg ttgccgtcct
     6601 cggcgtcgcg ctgctcggcg tcggcttcgt catcagcgcc ggcggcggcg cctcgggcgt
     6661 cgctcaggcc ggcatcgagc tcgtcaacag cgtgatccgg atcggcgcca acgccgtctc
     6721 ctttgcccgc ctcgccgcat tcggaattct ccacgccgcg ctcggactcg tcgtcttcgc
     6781 ggccgtcggc gcactctggg gcggcgtcgc cggctcgatc gcggcggtgc tggtcttcgt
     6841 gatcggcaac ctcgccgctt tctcggtcgg gctcctggtg accggcgttc aggctctccg
     6901 gctcgagttc tacgagatct tctcgaggat cttcgcaggt gaagggcacc cgttccggcc
     6961 ctgggccctt cctgtcgtgc aaccgaagga ggagtcatga tcctgtcgct actggtcgcg
     7021 ctacccgtca tcgtcctggc cgcggtcgtc accgtcaccg gaatacggcg cgcacccgag
     7081 cgcggcttca gacgtctcgt ggtgatcaac ggcgcgctcg cgctgggcgc cctcgggctt
     7141 ctggccgcga tcctggtcgg catcgtcgag cccgcgtctg cggccgagtc gacgagcagc
     7201 agtgatccac gggcggccct gatcggtgcg tcgatcgcga cggcgggatc cgctctcgga
     7261 gcaggcatcg ccgtctcgta cacgggctcc gcggccctcg ccgcgatcag cgagaagccg
     7321 gagatgttcg ggcgcgcgat ggtcatcgtc ggactcgccg aagggatcgc gatctacggg
     7381 ctcatcgtgt cgatcctgct cctgagctac gcatgagccg cgtcgtcgcg atcggcgagg
     7441 aggcgcggat cgccggctac gcactggccg gggcgaccgc ggtctccgct cgcgacgccg
     7501 ctgaagcgca ggccgcgtgg gaaacgctcc cacccgacac cgcactcgtc atcctcacgc
     7561 gggctgcgca cgaagcgctc gaggacgtcg tcggcgagcg ccgcctgctc tgggtggtgc
     7621 ttcctgaatg aatctcgagg cgctccgcgg ttcgtttctc gcagcagtgt cggaggatgc
     7681 agagcgcgcc gtctcggagt gcctgcgcga gtgccgggca cgaatcgcgg aggcgcaacg
     7741 acaggcggac gacgtcgtcg cgcgggcgcg cagcgccggc gagtccgact ccgagggcga
     7801 ggtcggccac attctcgtct ccgctcggcg tcaggcccag gcacgcgtgc tcgcggcgcg
     7861 acgcgaggtg tacgaggact tccgcgcgga ggcgctgcga gccgcgctcg cgctccgcgg
     7921 aacctcgcag tacgacgccc tgctcgcgcg cctcgaagac gatgtccggc gtcgactcgg
     7981 agaggatgcg cagctcgaga tcgatcccca cgacaggggc ggtgtgttcg gacgatccgg
     8041 ctcccgacgg ttggactact cgctcgcagc gctcgtggac gattgcgtct ccgagctggg
     8101 cgagcgagtc gagaggctgt ggacatgacc gacggtgccg tgcgaagagt caacggaccc
     8161 gtggtcgacg tcgacgggat cgacgttgcg atgctggatc tcgtcgaagt cggcgcgagt
     8221 cgcctcccgg gcgaggtgat cggcctctca gggccagtcg cgacggtgc
//
LOCUS       DBNGNK010000249         4137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1549135,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000249 DBNGNK010000000
VERSION     DBNGNK010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1549135"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="AC150V_07350"
     CDS             <1..1444
                     /locus_tag="AC150V_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762775.1"
                     /translation="ESGIQWVVASYSMTMAMFMMWGATFGDLRGRRFAYIAGIVLFSA
                     ASLACAFAPGIEFLSAARAIQGIGAAIVNVASLALVGAAFPEPKAKARAIGIWTGIAA
                     IGFTLGPVVGGVLTEHVGWESIFVFNPLVGAVVVVLTGLFVEESKDPTPRGFDKTGQV
                     LFIAGIGALTFGLIQGPQLGWLSPQIVAVFVFAALALVAFAYVELHKRQPMMDLRLFR
                     DRIYSTALYVVFAVLFCMYGTLLVITQYFQNVRDFSPEKAGLMIVAMGIPVVAIAPIT
                     GRIVARFGGRRPALLGLGCAIVGTGILAGSSASRLAVTLVGLLFLGVAAALTIAPATN
                     VAMATVAPERAGMASGILSVQRGLGSTAGFAIMGSVLAATVAIVLPGKLEPVIPNASE
                     RTEVVDQVVEDANPSAIASLIGPGNPLPEDTQTDDAALDAADDAFIIGIRVAMLVAFV
                     VVLSAFVLTWFTFPRRRAEPTPARPARASG"
     gene            1468..>4137
                     /locus_tag="AC150V_07355"
     CDS             1468..>4137
                     /locus_tag="AC150V_07355"
                     /EC_number="2.3.1.-"
                     /EC_number="6.2.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HIR3762776.1"
                     /translation="MSAYPTYMQTDIVLRDGSTVHVRPARPDDLERLEDYFIALSDES
                     RRLRFGGQTVDVREQARRSVEIDYDDHLTLLAFLGTDLSQVVGGAQYIRTGTARAEIA
                     MSVSDEFQGHGLASILIAHLAEAAQKNGIEFFYAEVLPENHRMTDVFRHSGFPISIRT
                     RPGTVEVEFPTTATEETASQYEERERQAAANAVRRVLEAKSIAVIGASRDPTTIGGRL
                     LRNLLGQPFAGVVYPVNPASEFVQGVQAYPTVRDIPGDVDLAFLVVPAAHVLDVALEC
                     SEKEVDSLVVISSGFGETGEEGRALQQRLLDVCRSTGMRLIGPNCMGIVNTDPAIAMN
                     GTFAQVYPPEGRIAFMSQSGALGIAVINMANSLGLGLSSFVSIGNKADVSANDLLCYW
                     AEDERTEVVLLYLESFGNPRRFARIVRRLGRRKPVVLVKSGRSSAGLRAASSHTGALV
                     ATSDTLVDALLRQVGVTRTDTLEEMFDVAALLANQPVPEGRRVAIITNAGGLGIQCAD
                     TCEARGLDVPELAPETVAELRSFLPSAAGVGNPVDMIASATGSDYGRTIRTVSVDPGI
                     DALIVLYIPPLEEDAPDVATHMVDAIGSIDRQIPIVTCFMSARGVPEALSAPGVRIPS
                     VAFPEQAAIALAHAADYGDWLRKPVGSVPDFPAVRKDEAAAVIAQALERGECWLEPDE
                     LTRLFDCYGIPLARQARAATPEEAAAAAAHLARPVALKAVGPVHKTDVGAVRLDLAAT
                     AVAAEAEAMAERVLSRGEPLEGFSVQEMVADGAEMLVGMTADPEFGPVVACGAGGVTV
                     ELVRDAAVRVAPVTDLEAKEMVRSLATFPLLDGFRGAPRRDVRALEDVILRVSALATD
                     QPAIAELDCNPVMVLDRGAVVVDARVRVRTRAPH"
BASE COUNT          604 a         1442 c         1400 g          691 t
ORIGIN      
        1 cgagtcgggc atccagtggg tggtcgcctc gtacagcatg acgatggcga tgttcatgat
       61 gtggggagcg accttcggcg atctgcgcgg ccgccggttc gcctacatcg ccggaatcgt
      121 cctcttctcg gcggcatcgc ttgcctgcgc gttcgcaccc ggcatcgagt tcctcagtgc
      181 agcccgggcc atccagggga tcggggccgc gatcgtcaac gtggcatcgc tcgcactcgt
      241 cggagcagcc tttcccgagc ccaaggcgaa agcccgggcg atcggcatct ggactggcat
      301 cgcggcaatc ggcttcacgt tgggaccggt cgtcggaggc gtcctcaccg agcacgtggg
      361 gtgggagagc atcttcgtct tcaacccgct cgtcggagcg gtcgtcgtcg tgctcaccgg
      421 cctcttcgtc gaggagtcga aggacccgac gccccgaggc ttcgacaaga ccggacaagt
      481 gctcttcatc gccggcatcg gtgcgctcac gttcgggctg atccaggggc cacagctcgg
      541 ctggctgtca ccacagatcg tcgcggtctt cgtcttcgcc gccctcgcgc tggtcgcatt
      601 cgcctacgtg gagctgcaca agcggcagcc catgatggac ctccgcctgt tccgcgatcg
      661 catctacagc accgcgctct acgtggtgtt cgcagtcctg ttctgcatgt acggaacgct
      721 gctcgtcatc acccagtact tccagaacgt gcgcgacttc agcccggaga aggccgggct
      781 gatgatcgtc gccatgggca tcccggtcgt cgccatcgct ccgatcacgg gtcgcatcgt
      841 ggcgaggttc ggcggtcgcc gcccggcgtt gctcggactc ggttgcgcga tcgtgggcac
      901 gggcattctc gccggcagca gcgcctcgcg tctcgccgtc accctcgtcg gactcctgtt
      961 cctgggggtg gccgccgcgc tcaccatcgc cccggcgacg aacgtcgcga tggccacggt
     1021 tgcgcccgag cgtgcgggaa tggcatccgg catcctcagc gtccagcgag ggctcggctc
     1081 caccgcgggc ttcgcgatca tgggcagcgt tctcgccgcc acggtcgcga tcgtcctgcc
     1141 cggcaagctc gagcccgtga tcccgaacgc atccgaacgg accgaggtcg tcgatcaggt
     1201 cgtcgaggac gcgaacccct ccgcgattgc ctctctgatc ggccccggca atcctctgcc
     1261 cgaagacacg cagaccgatg atgcggcact cgatgctgcc gacgacgcgt tcatcatcgg
     1321 catccgggtt gcgatgctcg tcgccttcgt cgtcgtgctg agcgcattcg tcctgacctg
     1381 gttcacgttc ccacggcgac gggcggagcc gacgccggcg cggcctgcac gcgcgtccgg
     1441 gtgagcgcgc gggcctagga tggcgacgtg tccgcctacc cgacatacat gcagaccgac
     1501 atcgtcctgc gcgacggcag caccgtccat gttcgacccg cgaggcctga cgatctcgag
     1561 cgcctcgagg actacttcat cgccctctcg gacgagtcgc gccggcttcg gttcggcggg
     1621 cagaccgtgg acgtgcgcga gcaggcccgg cgctcggtcg agatcgacta cgacgatcat
     1681 ctgacgctgc tcgcgttcct cggaaccgat ctctcgcagg tcgtcggcgg cgcccagtac
     1741 attcgcaccg gaacggcacg ggccgagatc gcgatgagcg tctccgacga gttccagggg
     1801 cacgggctgg cgtcgatcct gatcgcccac ctcgccgagg ccgcgcagaa gaacggcatc
     1861 gagttcttct acgccgaggt actgcccgag aaccaccgga tgacggacgt ctttcgtcac
     1921 agcggcttcc cgatctcgat ccggacgagg ccgggcaccg tcgaggtgga gttcccgacg
     1981 actgcgacgg aggagacagc gagccagtac gaggagcggg agcgccaggc ggcggcgaac
     2041 gctgttcgtc gcgtcctcga ggcgaagtcg atcgccgtga tcggtgcgtc gcgcgacccg
     2101 acgacgatcg gcggccggtt gctgcgcaac ctgctcggcc agccgttcgc cggcgtcgtc
     2161 tacccggtca acccggcaag cgagttcgtc cagggcgtcc aggcctaccc gactgtgcga
     2221 gacattcccg gcgacgtcga ccttgcgttt ttggtcgtcc cggcggcgca cgtgctggac
     2281 gtagcgctcg aatgcagcga gaaagaggtg gactcgctcg tggtgatctc gtccggattc
     2341 ggcgagacgg gcgaggaggg ccgcgccctg cagcagcggc tgctcgacgt ctgccgctcg
     2401 acggggatgc gcctgatcgg cccgaactgc atgggcatcg tcaacacgga tccggcgatc
     2461 gcgatgaacg ggaccttcgc gcaggtttac ccgccggagg ggcggatcgc gttcatgtcc
     2521 caatccggtg cgctcgggat cgcggtcatc aacatggcga actcgctcgg ccttggcctt
     2581 tcgtcgttcg tctcgatcgg caacaaggcc gatgtctcgg caaacgacct cctctgctac
     2641 tgggccgagg acgagcgaac ggaagtcgtc ttgctctacc tggagtcgtt cgggaatcca
     2701 cgacgcttcg cacgtatcgt ccggcgcctc ggccggagga agccggtcgt cctcgtgaag
     2761 agcgggcgta gctcggcggg gctgcgcgcc gcctcctctc acacgggagc gctcgtcgct
     2821 acgtccgaca cgcttgtcga tgccctgctg cgccaggtgg gcgtcacccg caccgacacg
     2881 ctcgaggaga tgttcgacgt cgcagcactc ctcgccaacc agcccgtgcc cgagggccgc
     2941 cgggtcgcga tcatcaccaa cgccggaggg ctggggatcc agtgcgcgga cacgtgcgag
     3001 gcacggggtc tggacgttcc cgaactggcg ccggagaccg ttgcggaact ccgctcgttc
     3061 ctccccagtg cggccggcgt cggcaacccg gtcgacatga tcgcctcggc gaccggcagc
     3121 gactacggcc gcacgattcg caccgtctcg gtcgaccccg gcattgacgc gttgatcgtc
     3181 ctctacatcc cgccgctcga ggaagacgcg cccgacgtcg cgacgcacat ggtggacgca
     3241 atcggctcga tcgaccgaca gatcccgatc gtcacgtgct tcatgtcggc gcgaggcgtg
     3301 ccggaagccc tgtcggcccc cggtgtgcgc atcccctcgg tcgcgtttcc ggagcaggcc
     3361 gcgatagccc tcgcgcatgc cgccgactac ggcgactggc tgcgaaagcc ggtcgggagc
     3421 gtgccggact tccccgctgt ccgaaaggac gaggcggccg cggtcatcgc gcaggcgctg
     3481 gagcggggcg agtgctggct cgagccggac gagctgactc gcctgttcga ctgctacggc
     3541 atcccactcg cgcggcaagc tcgcgccgcc acgcccgagg aggccgctgc ggccgccgcc
     3601 catctcgccc gccctgtcgc gctcaaggcg gtcgggccgg tgcacaagac ggacgtcgga
     3661 gcggtcaggc tcgatctcgc ggccaccgct gtcgcggccg aagccgaggc gatggccgag
     3721 cgagtcctct cccgcggcga gccgctcgag ggcttctccg tgcaggagat ggtcgccgac
     3781 ggcgccgaga tgctcgtcgg catgaccgcc gatcccgagt tcggtcccgt ggtggcatgc
     3841 ggcgccggcg gcgtcacggt ggagctcgtg cgcgatgcgg cggtgcgagt cgcgccggtg
     3901 acagatctcg aagccaagga gatggtgcgc tcgctcgcga cgttcccgct gctggacgga
     3961 tttcgaggtg cgcccaggcg cgacgtgagg gcgctcgagg atgtgatcct ccgcgtctcg
     4021 gctctcgcga ccgaccagcc ggcgatcgcc gagctcgact gcaaccccgt gatggtgctc
     4081 gaccgcgggg ccgtcgtcgt cgacgcccgc gtccgcgtcc ggacgcgggc accgcac
//
LOCUS       DBNGNK010000250         4568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_760550,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000250 DBNGNK010000000
VERSION     DBNGNK010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_760550"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..1765
                     /locus_tag="AC150V_07360"
     CDS             203..1765
                     /locus_tag="AC150V_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR3762777.1"
                     /translation="MAETHDTTESKLAWLAELRATALAGDEKAAERQRARGKLLARER
                     VEKLLDPGTFVELDRYVRHREVEFGMREQRPLGDAVVTGYGEVLGRKVFVFSQDFTVF
                     GGSLSEVFAEKVCKVMDLAVSFGCPIIGINDSGGARIQEGVVSLAGYAEIFWRNVQAS
                     GVVPQVSLVLGPCAGGAVYSPAITDFVLMAEGTSYMFITGPDVVKTVTGEEVSFEELG
                     GAATHAEKSGVAHLTAADEETCIEDARYLLSFLPQNNLETPPYAPPSDPIDREDPALD
                     GIVPDNPHRPYNMKAVIHSVVDDGDFLEIQPLWATNVVCGFARLAGHSVGIVGNQPGA
                     LAGVLDIDASVKAARFVRACDAFNIPLVTFVDVPGFLPGTAQEWGGIIRHGAKLLYAY
                     AEATVPKLTVITRKAYGGAYDVMSSKHIRADFNVAWPTAEIAVMGPEGAVNIVFRNEL
                     QRADDPEARRAELIAEYTERFANPYYAAERGYVDDVIEPRRTRPVLCAALEVALTKRE
                     SGPKRKHGNIPL"
     gene            1781..2158
                     /locus_tag="AC150V_07365"
     CDS             1781..2158
                     /locus_tag="AC150V_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR3762778.1"
                     /translation="MHRQFYTAAEAARALGVSIDTVRRWDRAGKLHVERDAANRRIVP
                     AAEVDRLRGVQETDRLSARNHLRGVVRSVTVDGLLAQVEIDVTEPTRLVAVITAESAK
                     ALGLAPGVSAAGVVKATSLMVER"
     gene            2173..2895
                     /gene="modA"
                     /locus_tag="AC150V_07370"
     CDS             2173..2895
                     /gene="modA"
                     /locus_tag="AC150V_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3762779.1"
                     /translation="MARALVAAGAVVVMTGCGGGGGESKATVLAASSLTEVLPEIAPG
                     ARYSFGGSNQLAEQVRQGAPFDVYVSASPRYTQELFREGLVREPTLFARNTLVLIVPE
                     SNPANLHDVHELARGSGTRLVAAGPEVPVGAYTRQVLERLGLAAVLDRAVSLEPDVKG
                     VVGKVALGEADAGFVYTTDVAAVVDEVSAIPLPASAQPAVGYEVAIAAEPDNPSAAAA
                     FVDGLLGPVGREQLRLRGFALP"
     gene            2892..3716
                     /gene="modB"
                     /locus_tag="AC150V_07375"
     CDS             2892..3716
                     /gene="modB"
                     /locus_tag="AC150V_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR3762780.1"
                     /translation="MSRGFSVLMWAAAVVALVFLLLPILALFLRVPPGELVRLMGSEA
                     AVEALVVTAKTNVAAMVLILLFGTPAAYFVATRPFRGRAVLVTLIELPLVLPPAVAGL
                     GLLAAFGRLGLLGSTLDVLGVEVAFTQAAVVMAVAFVASPFYLRTAVAAFESVDPSLP
                     AAARTLGAGPGRTFARVSLPLARAGLGAGAALAFARGLGEFGATIMFAGSLQGVTQTL
                     SLAIYQQFDVNFETALAIGAELVLVSAIVLLAVKFVPGWTRSDWISTTPSAASPSR"
     gene            3659..>4568
                     /locus_tag="AC150V_07380"
     CDS             3659..>4568
                     /locus_tag="AC150V_07380"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3762781.1"
                     /translation="MDTLRLDIDHALRSFAVALSLEVGRETVALVGPSGAGKSSVLRA
                     VAGLLRPDSGRIALGEDVWFDADRGIDRPPERRSVGLVFQDYALFPHMNVQENVSFGA
                     AGDVRPLLARLRIEGLADARPDALSGGEQQRVALARALAREPKVLLLDEPLSALDAHT
                     KGNVRGELLEVLRTLEIPTLLVTHDFDDAIALAPRVGVIVDGRIQQLGSPAELVAQPA
                     NEFVARFTGANLLAGVARPGSGGLTEVALDVGGVARTTDGGDGRVGLAVFPWDVALAR
                     EPADDSTLNHIRAPIVSLVSLGNRVRV"
BASE COUNT          654 a         1575 c         1583 g          756 t
ORIGIN      
        1 ccggaggcta accgccctcg cgcccgtcgc gtgagcggcc acccgtcgat cgtggagaag
       61 atcgacacga ctcggactca cacgcgcgtg ggcttcaacg ctaggctcga cgtgtgggga
      121 ggaggtcgga ggccccggac tggagtcccc acgagccccg tcttcggaag ccgacggaga
      181 agccggttag gctcccgccg acgtggccga aacgcacgac accaccgagt ccaagctcgc
      241 ctggctggcg gagttgcggg cgaccgcgct ggccggtgac gagaaagcag cggagagaca
      301 gcgtgcgcgc ggcaagcttc ttgcgcgcga gcgggtcgag aagctcctcg atccgggcac
      361 gttcgtcgag ctcgaccggt acgtgcgtca tcgcgaggtc gagttcggga tgcgagagca
      421 gcggcccctc ggtgacgcgg tcgtcacggg ttacggcgag gtgctcggga ggaaagtgtt
      481 cgtcttctcc caggacttca cggtcttcgg cggctcgctc tccgaggtgt tcgccgagaa
      541 ggtctgcaag gtcatggacc tggcggtctc gttcggctgc cccatcatcg gcatcaacga
      601 ctcgggaggc gcacggatcc aggagggagt cgtctcgctc gcgggctacg cggagatctt
      661 ctggcggaac gtccaggcct cgggggtggt gccgcaggtc agtctcgtgc tcgggccgtg
      721 cgcgggtggc gccgtgtatt cgccggcgat cacggacttc gtcctcatgg ccgaggggac
      781 ctcgtacatg ttcatcaccg gccccgacgt cgtgaagacg gtgaccggcg aggaagtgag
      841 cttcgaggag ctgggcggag cggcgaccca cgccgagaag tccggcgtcg cccacctcac
      901 cgccgccgac gaggaaacct gcatcgagga cgctcgctac ctcctgtcgt tcctgcccca
      961 gaacaatctc gagacgccgc cctacgcgcc gccgtcggac ccgatcgatc gcgaggatcc
     1021 cgcactcgac ggcatcgtcc ccgacaaccc gcatcgtccg tacaacatga aggccgtaat
     1081 ccacagcgtc gtcgacgacg gcgacttcct cgagatccaa ccgctctggg ccacgaatgt
     1141 cgtctgcggc ttcgcacgcc tggccggcca ctcggtcggg atcgtcggga accagcccgg
     1201 cgccctcgcg ggcgtgctcg acatcgacgc gtcggtcaag gccgctcggt tcgtccgcgc
     1261 ctgcgacgcc ttcaacatcc cgctcgtcac gttcgtggac gtcccgggtt tcctgccggg
     1321 aaccgcgcag gagtggggcg gcatcatcag gcacggggcc aagctcctct acgcgtatgc
     1381 cgaggcgacc gtcccgaagc tgacggtgat cactcgaaag gcatacggcg gggcgtacga
     1441 cgtcatgagc tccaagcaca ttcgcgccga cttcaatgtt gcctggccga cggccgaaat
     1501 tgcagtgatg gggcccgaag gagccgtcaa catcgtcttc cggaacgagc ttcagcgagc
     1561 ggacgatccg gaagcgcgtc gcgcggagct catcgccgag tacacggagc gcttcgccaa
     1621 tccgtactac gccgccgagc ggggctacgt cgacgacgtc atcgagccgc gccgtacacg
     1681 gccggtgctc tgcgccgcgc tcgaggtcgc gctgaccaaa cgcgagtcgg gcccgaagcg
     1741 caagcacggc aacatcccgc tctgacatac ttcatatgca atgcataggc agttctacac
     1801 ggccgccgag gctgcgcgtg cgctcggggt gtcgatcgac actgtccgcc gctgggaccg
     1861 ggccggcaag ctccacgtcg agcgggacgc cgccaaccgg cgcatcgttc ccgccgcaga
     1921 ggtagaccgc ctccgcggcg tgcaagaaac cgaccggctg tccgcgcgta accatctgcg
     1981 cggggtcgtc cgttcggtca ccgtcgacgg cctgctcgct caggtcgaga tcgacgtcac
     2041 cgagccgacg cgactcgtcg ccgtgatcac ggccgagtcg gcgaaggcgc tgggcctcgc
     2101 gcccggagtc tcggccgcgg gagtcgtgaa ggccacatcc ttgatggtcg agcggtgatg
     2161 cgcggccgat gtgtggctcg cgcgctcgtc gcggccggcg ctgtcgtcgt catgaccggc
     2221 tgcggcggcg gtgggggcga gagcaaggcg acggttctcg ccgcctcttc gctcacggag
     2281 gttctgccgg agatcgcgcc gggagcccgc tattccttcg gtggctccaa ccagcttgcg
     2341 gagcaagtgc gtcagggcgc gccgttcgac gtctacgtct cggcgagtcc tcgctatacc
     2401 caggagcttt tccgagaggg gctcgtccgg gagccgactc tattcgcgcg caacacgctc
     2461 gtcctcatcg tcccggaatc caacccggcg aacctgcacg acgtccacga gctcgctcgg
     2521 ggatcgggca cgaggctcgt cgcggcggga cccgaggtgc cggtcggcgc ctacacgcgg
     2581 caggttctcg agcggctcgg cctcgccgct gtcctcgatc gggcggtaag cctcgagccc
     2641 gatgtcaaag gcgtcgtagg caaggtggca ctgggcgagg cagacgccgg cttcgtctac
     2701 acgacggacg tcgcggccgt ggtcgacgag gtgagcgcga ttccgctccc cgcctcggcc
     2761 cagccggccg tcggctacga ggtcgccatc gcggccgagc ccgacaaccc atcggcggcc
     2821 gctgctttcg tcgacgggct gctggggccc gtggggcggg agcagcttcg cctacgagga
     2881 ttcgcgctac cgtgagtcga gggttcagcg tcctcatgtg ggctgcggcg gtcgtcgccc
     2941 tcgtgtttct cctgcttccg atcctcgccc tcttcttgcg tgtcccgccg ggggagctcg
     3001 tccgtctgat gggaagcgag gccgcggtcg aggcgctcgt ggtgaccgcc aagacgaacg
     3061 tagcggcgat ggtcttgatc ctgctcttcg ggacgccggc cgcgtacttc gtggccactc
     3121 gtccgttccg cggccgagcc gttctcgtga cgctgatcga gctgccgctg gtgctgcccc
     3181 cggccgtcgc cgggctgggg ctgttagctg cgttcggtcg tctcggtctc ctcggcagca
     3241 cgctcgacgt cctcggagtc gaggtggcat tcacgcaggc cgcggtggtc atggcggtcg
     3301 ccttcgtggc aagccctttc tacctgcgca ccgcggtcgc cgctttcgag tcggtcgatc
     3361 cgtctctccc agcggcggcc cggacgctcg gcgccgggcc gggtcggacc ttcgcccgcg
     3421 tgtcgctccc tctcgcgcgc gcggggctcg gtgccggagc ggccctcgcc ttcgcgcgag
     3481 gactcggcga gttcggcgcc acgatcatgt tcgcggggag tctccagggc gtgacgcaga
     3541 cgctctcgct cgccatctat cagcagttcg acgtcaactt cgagaccgca ctcgcgatcg
     3601 gggccgagct cgtcctcgtc agcgcgatcg tgctgctcgc ggtcaagttc gtaccagggt
     3661 ggacacgctc cgactggata tcgaccacgc cctccgcagc ttcgccgtcg cgctgagcct
     3721 cgaggtaggc cgcgagacgg ttgcgctcgt gggcccgtcg ggcgcgggca agtccagcgt
     3781 gctacgggcg gtcgcgggtc ttctgcgacc cgactcgggg cgcatcgccc tcggtgaaga
     3841 cgtctggttc gacgcagacc gcgggatcga tcgccctccc gagagaaggt cggtcgggct
     3901 ggtcttccag gactatgcgc tcttcccgca tatgaacgtg caagagaacg tgtcgttcgg
     3961 tgcggcgggc gacgtccgcc ctctcctcgc gcggctccgc atcgaggggc tcgctgacgc
     4021 tcggcccgat gcgctctccg gcggcgagca gcagcgcgtg gcactcgcga gagccctcgc
     4081 ccgcgagccc aaggtgctcc tgctcgacga gccgctgtcg gcactcgacg cgcacacgaa
     4141 ggggaacgtc cggggcgagc ttctcgaggt gctccggaca ctggagatcc cgactctgct
     4201 cgtcacacac gacttcgacg acgcgatcgc gctggcgccg cgggtcggcg tgatcgtcga
     4261 cggtcggata cagcagctgg gcagtccggc cgagctcgtc gcgcaaccgg cgaacgaatt
     4321 cgtcgcgcgc ttcacgggtg ccaatctgct ggccggagtg gcgcgtcccg gcagcggcgg
     4381 cctgacggag gtcgcactcg atgtcggcgg cgtcgcccgc acgaccgacg ggggtgacgg
     4441 gagggtcggg ctggccgtct ttccatggga cgtcgcgctc gcccgcgagc cggcggacga
     4501 ctcgacgctg aaccacattc gggcgccgat cgtctcgctc gtctcgctcg gcaaccgcgt
     4561 ccgcgtcc
//
LOCUS       DBNGNK010000251         3066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1764676,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000251 DBNGNK010000000
VERSION     DBNGNK010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1764676"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="AC150V_07385"
     CDS             <1..367
                     /locus_tag="AC150V_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3762782.1"
                     /translation="AVEWSLRLATIWFLLDAFGIEQSVQNVLLVQVSMSLATIVPLTP
                     AGIGTEQALLLYVFRGATSRSQLLAFSVGMKITLSVVNLAVGLAAIALTLRTVRIGRA
                     IDTAREHEAAQNPTGEEGG"
     gene            complement(364..1110)
                     /locus_tag="AC150V_07390"
     CDS             complement(364..1110)
                     /locus_tag="AC150V_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762783.1"
                     /translation="MKALVSALVTVAAVLAFVVAACGDGGGESTATGVTETAPVVDDY
                     PADPEAAETLDRFIQAAGAGDTAAMWNLLDSRSQQRFGPTEQLWSDGSGGDLAVVLGS
                     FANEGGGYELVLSRRISDLWSVAAVQGFVPAGEDTEWGAYAAVVGHESGERKISLAGT
                     ATFNPVVPEPELKSDATPDVATEVEAAEPILTAALWVDETLLATDVSPDELFLTSRVT
                     TPLEPKRHTIVSYADTQSGAGANAWTFVSR"
     gene            complement(1107..2963)
                     /gene="uvrC"
                     /locus_tag="AC150V_07395"
     CDS             complement(1107..2963)
                     /gene="uvrC"
                     /locus_tag="AC150V_07395"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007575122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR3762784.1"
                     /translation="MDQQLRDRLGSLPTGPGVYLFRGGKDEVLYIGKAKSLRARVRTY
                     WNRSGDSRVGIERLLGRIHDVEVIVTQTEAEALHLEQNLVKRHRPPFNVRLRDDKSFP
                     YIAVTVGDDYPRVMFTRERHRRGTVYFGPYANAKKVRETLDVLNRAFRFRPCEGPKPG
                     RHSGIPCLDYHIDRCTAPCVGAITMADYRATIDGVIEFLSGETRPIVRELEGRMKEAA
                     AAERFEEAARYRNRLAAVQSLAERQTADKRAVGTVDVIGVAVEGEAATVQVFPLRDGK
                     LIDRYSFHLENVGGQDVATILESFFLEYYGSAPSVPPEILAPPEVGDTTALERFLSDV
                     RGARVEVRTPLRGEKRRLAEIAGENARLALESEATRAEQSRLRRIESLEQLREGLNLE
                     SLPVRIECFDISNIQGSSIVGSMAVFQDAVPKKAHYRSFAVRGQEGQDDYSALAEVVS
                     RRFARMRLVTDGKWDESFAAVPNLVVIDGGKGQLAAALDAVQAFDLPRTAVIALAKRQ
                     EEVFVPGHQSAIVLDRHDAGLQLLQRARDEAHRFALSYHRRRRDTEAFESIFNTLQGV
                     GPARRRAILRHFGSAERFLAASQEELEGVPGLPAKTARAVYAQLHKTGGVAT"
BASE COUNT          451 a         1078 c         1039 g          498 t
ORIGIN      
        1 ggcggtggag tggtcgctcc ggctggcgac gatctggttc ctgctcgacg cgttcggaat
       61 cgagcaatcg gtgcagaacg tcctcctcgt ccaggtgagc atgagcctcg cgacgatcgt
      121 gccgctgacg ccggcgggca tcggcacgga gcaggcgctc ctgctctacg tcttccgcgg
      181 ggcaacgtcg cgctcccagc tgctcgcctt cagcgtcggc atgaagatca ccctcagcgt
      241 ggtgaacctc gccgtcggtc ttgcggcgat cgcgctcacg ctccggaccg tgcgcatcgg
      301 tcgcgcgatc gacaccgccc gtgagcacga ggcggcccag aacccgaccg gcgaggaggg
      361 cggctagcgg gagacgaacg tccaggcgtt ggcgccggcg ccggactgcg tgtcggcgta
      421 cgagacgatc gtgtgcctct tcggctcgag cggcgtcgtc accctgctcg tgaggaagag
      481 ctcatcgggc gacacgtcgg tggcgagcag cgtctcgtcg acccacaggg cggcggtcag
      541 aatcggctcg gctgcctcga cctcggtcgc gacgtccggc gtcgcatccg acttcaactc
      601 gggctccggc accacggggt tgaaggtggc cgtgccggcg agcgagatct tccgctcccc
      661 gctctcgtgc ccgaccaccg ccgcatacgc accccactcg gtgtcctccc ccgccgggac
      721 gaagccctga accgccgcga ccgaccagag gtccgatatt cgccgtgaca gaaccaactc
      781 gtagccgccg ccctcgtttg cgaaggagcc cagcacgact gcgaggtcgc cgccagagcc
      841 gtccgaccag agctgctcgg tcggcccgaa ccgctgctgt gaccggctgt cgagcaggtt
      901 ccacatcgcg gcggtgtcgc ccgcccctgc ggcctggatg aaccggtcga gcgtctccgc
      961 cgcctcggga tccgcggggt agtcgtccac aaccggcgcg gtctccgtca cgccggtcgc
     1021 cgtcgattcg ccgccgccgt ctccacacgc tgcaacgacg aaggccagca cggccgcgac
     1081 ggtgacgagg gccgagacga gagccttcat gtcgccacgc cgcctgtctt gtgcagctgc
     1141 gcgtacacgg cgcgagccgt tttggccggc agtccgggaa ctccttccag ctcctcctgg
     1201 gaggcggcga gaaaccgttc ggccgagccg aagtggcgca ggatcgccct gcggcgggcc
     1261 ggcccgacgc cctgcagcgt gttgaagatc gactcgaacg cctctgtgtc ccgccgccgg
     1321 cggtggtagg agagagcgaa gcggtgagct tcgtcgcgag cccgctgcag gagctggagc
     1381 ccggcgtcgt gccggtcgag cacgatcgcc gattggtgcc ccggcacgaa cacctcctcc
     1441 tggcgtttcg cgagcgcgat cacggccgtt cgcggaagat cgaaggcctg aaccgcgtcg
     1501 agcgccgcgg cgagctgccc cttgccgccg tcgatgacca cgaggttcgg gacggcggcg
     1561 aagctctcgt cccacttgcc gtcggtcacg agccgcatac gcgcgaagcg gcggctcaca
     1621 acctcggcga gggcgctgta gtcgtcctgt ccctcctgcc cgcggaccgc gaagctgcgg
     1681 tagtgggcct tcttcggaac cgcgtcttgg aacaccgcca tcgatccgac gatggaggag
     1741 ccctgaatgt tggagatgtc gaagcattcg atccgaaccg gcaggctctc gaggttgagc
     1801 ccttcgcgga gctgttcgag cgattcgatc cggcggagcc gcgactgctc cgctcgcgtc
     1861 gcctccgact cgagcgcgag gcgggcgttc tcgcccgcga tctcggcgag ccggcgtttc
     1921 tctccgcgca gcggcgttcg cacttcgacg cgggcgccgc gcacatcgga caggaaccgc
     1981 tcgagtgccg tcgtgtcgcc cacctccggc ggagcgagga tctcgggcgg gacgctcgga
     2041 gccgacccgt agtactcgag gaagaaggac tcgaggatcg tcgcgacgtc ctggccgccg
     2101 acgttctcga ggtggaacga gtatcggtcg atcagcttgc cgtcccgcag cggaaagacc
     2161 tgcacggtcg cggcctctcc ctcgaccgcg acgccgatca cgtcgacggt tccgaccgcg
     2221 cgcttgtccg ccgtctgccg ctcggcaagg ctctgaaccg ccgccagccg gttgcggtac
     2281 cgcgccgctt cttcgaaacg ctctgccgca gcggcctcct tcatccggcc ttcgagctcc
     2341 cggacgatcg ggcgcgtctc cccggagagg aactcgatca cgccgtcgat cgtcgcccgg
     2401 tagtccgcca tcgtgatcgc gccgacgcac ggcgccgtgc agcggtcgat gtggtagtcg
     2461 agacacggga tccccgaatg gcgacccggc ttcggcccct cgcaggggcg aaagcggaac
     2521 gcgcgattaa gtacgtcgag cgtctcccgc accttcttcg cgttcgcgta cgggccgaag
     2581 tacacggtgc cccggcggtg tcgctcgcgc gtgaacatca cgcgtggata gtcgtcgccg
     2641 accgtcacag cgatgtacgg gaacgacttg tcgtcgcgga ggcggacgtt gaacggcggg
     2701 cggtggcgtt tgacgaggtt ctgctcgagg tggagcgcct ccgcctcggt ctgcgtgacg
     2761 atcacctcga cgtcgtggat ccggccgagc agccgctcga tcccgacgcg gctgtccccg
     2821 cttcggttcc agtaggtgcg gacacgggcg cgcagcgact tcgccttgcc gatgtacagc
     2881 acctcgtctt tgccgccgcg gaagaggtag acgcccggcc ccgtgggcag cgacccgaga
     2941 cggtcgcgga gttgctggtc catcccggca agcgtacccg tggtgttctg cgtaccattg
     3001 ttgacgtgtc cgaggaacct cactacgagc ggatcgagga cctcccgagc ggtcagtacg
     3061 tctcct
//
LOCUS       DBNGNK010000252         4100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_833984,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000252 DBNGNK010000000
VERSION     DBNGNK010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_833984"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="AC150V_07400"
     CDS             <1..365
                     /locus_tag="AC150V_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762785.1"
                     /translation="IEDFLFDQGFEVVLPAMEGDETEIREDHKENLLLCDAVLIYCGS
                     ASEAWLRAQLRELMKAPGFGRDKPMLAKAIYLGSPLSPAKERFRTREAEVIKGAEQFS
                     PPSLASFIAALRGSKADA"
     gene            388..>4100
                     /locus_tag="AC150V_07405"
     CDS             388..>4100
                     /locus_tag="AC150V_07405"
                     /inference="COORDINATES: protein motif:HMM:NF044287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR3762786.1"
                     /translation="MEASGQFFCPFPGLRPFDADEAHLFFGREGQSTELLRLLREHRF
                     LAVLGSSGSGKSSLVRAGLLPSLYGGFMRSAGSDWRIAVMRPGTDPITKLAATLADPS
                     ALGAGDVEPAMQSTLIEATLRRGSLGLVEAVKESRLPEGQNLLVVVDQFEEIFRFRAQ
                     SGDTDVKDDAAAFVKLLLEAGAAPGARIYVILTMRSDYLGDCSQFRDLPEAINRGKYL
                     IPRMTREQRRLAIEGPVAVGGGMIAPRLVNRMLNDMGSDPDQLPILQHAAMQTWKRWA
                     DEGKPDEPLDLEHYDAIGGMDTALSLHADKAFEELRDLRKREIAEKIFRRLTERGADN
                     REIRRPTRFADLCAVTGASKEAVAGVIEVFRQPGRSFLMPAAGTELTASSLIDISHES
                     LIRGWDRLAAWVEEEGESAKVYKRLADTAALWKQDRAGLWRDPDLQFALDWWERDKPN
                     AAWAAAYDPDFVLAKHFLDKSREARDAAARARKRQIRVVIAALAIALAGTSMLAVWAL
                     ASRRAAVRAEAVAVERQQAADSLLILANESAERARTMQGRADSSATEARLFAEQVEQQ
                     NTQLIAQRGALAVARDSARGAAQLAEFHGVRAEAAERRTRIEQVSTRGAAVSRLDELE
                     LESRDEHPLEAINLRKTRAGNFSNMERWREAIDEYSLALRGNPSDIGALVNRSDAYLN
                     MGKPDSARADLEYALVRNPHYFLAHANMALALGYLGDYEGARRHYRSAMVSTAYEAGE
                     TSQSKVSPDLYRVMGLRGLTLDGSEANSALAYGLASTYAFEGGDDFDGFDSALAAADG
                     VRRSQDAVLSALNWAWLHRDYRMTEYGTHAAEGALWARAGEASGDEVARIFQQEAVRS
                     YCRFLAAHELSGEARYDDVAVWVDGQLSGTGGRCRGVVLEPGLEEPSDVATLLVDANE
                     LGARGDLLGADAVLTRALAREPDNPQIILDRANVRRELVEATALRDSALTADIERGTD
                     QAVGYESKIRKQLQTPGAPGEADWQDSLAVWRRSLAAVQARVSARRDSLALLSENADA
                     WYDSATVDANRALEISPYLSEAYYIRGRVLYEKATWDDLLDPAIVDDMVEQFDLALTY
                     DPTDGNAHTFRALALKRTDPAESAESYRKALETYPQLAWMWQDLAEMEYAQDRYVEAE
                     RAMDVAIGILPERLSYHDDRAVILDAMGRAPVEVELERAAGYRRAALEIYGNGNADSA
                     RSALGGSAGILLLLAEAEPTADIRASLDRSINIG"
BASE COUNT          669 a         1365 c         1470 g          596 t
ORIGIN      
        1 cgatcgagga cttcctcttc gaccaggggt tcgaggtcgt cctgcccgcg atggagggag
       61 atgagaccga gatccgcgag gaccacaagg agaacctcct cctgtgcgac gcggtcctca
      121 tctactgcgg gtcggcgagc gaggcgtggc tgcgggcgca gctccgcgag ctcatgaaag
      181 cccccggctt cgggcgcgac aagccgatgc tggccaaagc gatctacctc ggttcgccgc
      241 tttcgccggc caaagagcgc ttccgcaccc gcgaggcgga agtgatcaag ggcgccgagc
      301 agttctcgcc cccgagcctc gcgtcgttca tcgccgctct ccgcggctcg aaggccgacg
      361 cgtgacgggt ccgagggtgg gcagctgatg gaagcgtccg gacagttctt ctgcccgttt
      421 cccggtcttc gccccttcga cgcagacgag gcgcaccttt tcttcgggcg cgaagggcag
      481 agcactgagc tgctgcggct gctccgggag catcgcttcc tggccgtgct cggttcgtcg
      541 ggaagcggca agtcgtctct cgtccgggcg ggtctgctcc catcgctgta cggcggattc
      601 atgcgctccg ccgggtccga ttggcgcatc gccgtcatgc ggccgggcac cgacccgatc
      661 acgaagctgg ccgcgacgct cgcggacccg tcggccctcg gcgcgggtga cgtcgagccg
      721 gccatgcaga gcacgctgat cgaggcgacg ctgcgccgcg gcagcctcgg cctggtggag
      781 gcggtgaagg agtcgcggct tcccgaagga cagaatctgc tcgtggtggt ggatcagttc
      841 gaggagatct tccgcttccg ggcgcagtcc ggcgacacgg acgtgaagga cgatgccgcg
      901 gccttcgtca agctgctgct ggaggcgggg gcggcgcccg gcgcccggat ctacgtcatc
      961 ctcacgatgc gctccgacta tctgggcgac tgttcgcagt tccgcgacct gcccgaagcg
     1021 atcaaccgcg ggaagtatct catcccgcgc atgacgcgcg agcagcggcg actggcgatc
     1081 gagggaccgg tggccgtcgg cggcggaatg atcgcgccga ggctggtgaa ccggatgctg
     1141 aacgacatgg ggagcgaccc cgaccagctc ccgattctcc agcacgccgc catgcagacg
     1201 tggaagcgct gggccgacga gggcaaaccg gacgagccgc tcgacctcga gcactatgat
     1261 gcgatcgggg gcatggacac cgcgctctcg ctccacgccg acaaggcgtt cgaggagctg
     1321 cgcgacctgc gcaagcgcga gatcgccgag aagatcttcc ggcgcctcac cgagcgcggc
     1381 gccgacaatc gcgagatccg ccgcccgacc cgattcgccg acctctgcgc cgtgaccggc
     1441 gcgtcgaagg aggcggtcgc cggcgtgatc gaggtattcc gccagccggg ccgctcgttc
     1501 ctgatgccgg cggcgggcac ggagctgacg gccagctccc tcatcgacat ctcgcacgag
     1561 agcctgattc gcggctggga ccggctggcg gcgtgggtcg aagaggaggg cgagtcggcc
     1621 aaagtgtaca agcgcctggc cgacacagcg gcgttgtgga agcaggaccg ggccggactg
     1681 tggcgcgacc ccgaccttca gttcgccctc gactggtggg agcgcgacaa gccgaacgcc
     1741 gcctgggcgg cggcctacga tcccgacttc gtgctcgcca agcacttcct ggacaagagc
     1801 cgcgaggcgc gcgacgccgc ggcccgcgcc cggaagcgac agatccgcgt ggtaatcgcg
     1861 gcgctcgcga tcgctctcgc cggaacatcg atgctggccg tgtgggcgct cgccagccgc
     1921 cgcgcggccg tgcgcgcgga ggccgtggcg gtggagcgtc agcaggccgc cgattctctc
     1981 ttgatcctcg ccaacgaaag cgccgagcgc gcgcgcacca tgcagggacg cgccgattcg
     2041 tccgccaccg aggcccgttt gttcgccgag caggtcgaac aacagaacac gcagttgatc
     2101 gcgcagcggg gcgcgctggc cgtggcgcga gattccgccc gtggcgcggc gcagctggcg
     2161 gagttccacg gcgttcgtgc cgaggccgcc gagcgccgca cccggatcga gcaggtcagc
     2221 acgcggggtg cggccgtgag ccgtctggat gagctggagc tcgagtcgcg ggacgaacac
     2281 ccgctcgagg cgattaacct gcggaagacg cgcgccggga acttcagcaa catggagcgc
     2341 tggcgggaag cgatcgacga gtactcactg gctctgagag gcaacccgag tgacatcggg
     2401 gcgctcgtca accgaagcga tgcctacctc aacatgggca agccggacag cgcccgcgcc
     2461 gacctcgagt acgcgctcgt ccggaatccg cactacttcc tcgcacacgc caacatggct
     2521 ctggcgctcg gctacctggg agactacgag ggggcgcgcc gccactaccg gtcggccatg
     2581 gtatcgaccg cgtacgaggc gggcgagacc agccaatcca aggtatcgcc cgatctgtac
     2641 cgggtgatgg gcctgcgggg gctgacgttg gatgggagcg aggcgaacag tgccctcgcg
     2701 tacgggctcg cgagcacgta cgccttcgag ggcggcgacg acttcgacgg attcgactcc
     2761 gcgctcgcgg ctgccgacgg ggtccgccgc tcacaggacg cggtgctgag cgcgctgaac
     2821 tgggcgtggc tgcaccgcga ctaccggatg actgaatacg gcacgcacgc ggccgagggc
     2881 gcactctggg cgcgcgcggg tgaggcgtcc ggcgacgagg tggcgaggat cttccaacag
     2941 gaagcggtcc gctcgtactg ccgattcctg gctgcccacg agctgagcgg agaagcccgc
     3001 tacgacgacg tggccgtgtg ggtggacggc cagttgtccg ggaccggcgg ccgctgccgc
     3061 ggcgtcgtgt tggagccggg cctcgaggag ccgtccgacg tggcgactct gctggtcgac
     3121 gcgaacgagc tgggggctcg gggcgacctg ctgggcgccg atgccgtgct gacgcgtgct
     3181 ctcgcgcgcg agccggacaa cccgcagatc attctcgatc gagcgaacgt gcggcgggag
     3241 ctggtcgagg cgaccgcgct gcgggacagt gccctcaccg ccgacatcga acggggcacc
     3301 gaccaggcgg tcggctacga gagcaagatc cgcaaacagc tccagactcc cggggcgccg
     3361 ggggaggccg attggcagga ttccctggcc gtgtggcgcc gctccctggc agcggtccag
     3421 gcccgggtga gcgcgcggag agattcgttg gcattgctga gcgagaacgc cgacgcgtgg
     3481 tacgactcgg ccaccgtcga tgccaaccgc gcgctcgaga tctctcccta cctgtccgaa
     3541 gcgtactaca tccgcgggcg cgtgttgtac gagaaggcga cgtgggacga tctcctcgat
     3601 cccgcgatcg tcgatgacat ggtcgagcag ttcgacctcg cgttgacgta cgacccgacc
     3661 gacggcaacg cgcatacgtt ccgtgcgctg gccctgaagc gtacggaccc cgccgagagc
     3721 gcggagtcgt accgaaaggc gctcgagacg tatccgcagc tcgcctggat gtggcaggac
     3781 ctggcggaaa tggagtacgc gcaggatcga tacgtcgaag cggaacgcgc gatggacgtc
     3841 gcgatcggga tcctgccaga gcgcctgagc taccacgacg atcgggccgt catcctcgac
     3901 gcgatgggcc gtgcgccggt cgaggtcgag ctcgaacggg cggccggcta ccgacgcgcg
     3961 gcgctggaaa tctacggaaa cgggaacgcc gacagcgctc gctccgcgct gggggggagc
     4021 gccggtattc tcctcctgtt ggcggaggcc gagcccacgg ccgacatccg cgcatctctc
     4081 gatcgctcga tcaatatcgg
//
LOCUS       DBNGNK010000253         9469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1764862,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000253 DBNGNK010000000
VERSION     DBNGNK010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9469
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1764862"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="AC150V_07410"
     CDS             complement(<1..822)
                     /locus_tag="AC150V_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3762787.1"
                     /translation="MRILLVSQMYPGPDAPDLGTFVAALERKLVERGHEVERAVLDRR
                     GERGRYARLLRETEASSRRFRPDVVYAHFLVPTGLWAVLAGRAPVVVTAHGQDVENAR
                     TRSAVRAATRLVVRRSAAVVAVSDWLRRRLEAAVPEATGKTAVVDCGVDNARFADLEQ
                     DKARDEVGWHPDGTAFVCVGSLTERKNVVRLARAFAKHGKGSLAFVGDGPLRPELVGI
                     PGVLLAGAVPHERAAAWIAASDVLCQPSLVEPFGIAALEGLAAGKPVVATTVGGPP"
     gene            complement(863..2440)
                     /locus_tag="AC150V_07415"
     CDS             complement(863..2440)
                     /locus_tag="AC150V_07415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR3762788.1"
                     /translation="MAATDRRETDSGIEIKPVYGAADAPTGGLEPPGEYPFTRGPYST
                     MYRGRPWTIRQYAGFASAEESNERYRYLLERGQTGLSIAFDLPTQLGLDSDDPLAEGE
                     VGRTGVAIDSIDDMHVLLDGIPLDEVSTSMTINAPAALLLLLYALVAEEQGVGGDQLR
                     GTVQNDILKEYVARGNYIFPPRPSMRLTTDLFAYCEAEIPQWNTISISGYHIREAGST
                     AAQELAFTLANGIAYCDAAVQSGLSPDRFGERLSFFFNAHNNFFQEVAKFRAARRLWA
                     RIMRERFGATNVKAQALRFHAQTGGSTLTAQQPENNIVRVAVQALSAICGGAQSLHTN
                     SFDEALALPTEHAARIALRTQQILMDEAGTIDTADPLGGSFYIEALTDELERLAQALI
                     DEIDERGGAVAAIEQGVVQEQIEEASFRHQARVEAGEQVIVGVNRFTETATEHVELLT
                     VDPESERLQRERTAAVRAQRDPNAAERALAEVRRVAVGDENLLVPMREALRARCTVGE
                     ICGVLRELWGTYDAQRV"
     gene            complement(2464..3120)
                     /locus_tag="AC150V_07420"
     CDS             complement(2464..3120)
                     /locus_tag="AC150V_07420"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3762789.1"
                     /translation="MTVTLPRTNLNEQVYENLRARLLTRELGPGAKLSLHELADELGV
                     SRSPVHHALTRLVSEGLLTVKARRGYFVTPVTAEVVAEGYDVRLALELHAALNTVGRL
                     APERLARFRSLMEATAAALSHDEWDTANAAFHEYQVDLAENHLLSRFYRELSVNLMMQ
                     VIRGGRVERSKELATGHARIVDAFESGDVDSAQQAVRDHLEAGKRIALDAIERAGGVL
                     "
     gene            3176..3859
                     /locus_tag="AC150V_07425"
     CDS             3176..3859
                     /locus_tag="AC150V_07425"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein"
                     /protein_id="HIR3762790.1"
                     /translation="MDVGVRLFAGLRDRAGVGRVDVRLPEGATVADVWPALDLGEEPS
                     GLLYAVNREYAEASAALSDGDEVAVIPPVSGGDFRLSAEPLSVDRVVAEVASEAAGAV
                     ATFIGTTRARSRDRDVVRLEYEASEGMAEAELERIAQSLQQRYELVGVAIHHRVGVVG
                     VGETSLVIAVSAAHRADALAACRDAIDELKVSVPLWKKEVYAGGEEWIGSEADYQAEL
                     LAERESAGG"
     gene            complement(3945..4235)
                     /locus_tag="AC150V_07430"
     CDS             complement(3945..4235)
                     /locus_tag="AC150V_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762791.1"
                     /translation="MRRITRRLLVAATVIAALAVTVGAQAEPTDKVLLCHGTASAKNM
                     YVLIEVSGNAIPQGHQPGAQGHGDKNYPDMIFRSDLYDDCEAQYDATIGGEE"
     gene            4410..5102
                     /locus_tag="AC150V_07435"
     CDS             4410..5102
                     /locus_tag="AC150V_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR3762792.1"
                     /translation="MSGADPYEPRSAPEPEQYSPLKPQRGAVDWLKRLAAPLVVLGGL
                     LVKFGGSLKFLGIFVAVGGYALIWGWAFGVGFVLLILAHELGHFVEAKRQGLSPSLPV
                     FVPFLGAFVAIKGAPFDPWRNGLVSIAGPVAGAAAAFGVLVAGDLYDSRFLLALAYVG
                     FLLNLINLAPFAILDGGYIARSWRLLRAGAGAMTADEARRKAWLLGGAYVVIAGLLVV
                     GMIVAHVPQDRL"
     gene            5099..5800
                     /locus_tag="AC150V_07440"
     CDS             5099..5800
                     /locus_tag="AC150V_07440"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010592459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR3762793.1"
                     /translation="MTDDRPHENGLDRRLLRRTHETLASDVSLIASEFLAGFQAVQEI
                     DRPAVSIFGSARVEEGTPVYELAREVGAGFAAQGFAVVTGGGPGVMEGANRGARDAGG
                     LSVGFNIVLPHEQSMNPYVGHGVTFKHFYARKTMFVKAAEGFVIFPGGFGTMDELYES
                     LTLIQTNVIGYFPVVLFDSDYWAEMLHFLRVEMLEDGLVSPEDLELLYLTDSPAEAVD
                     LVVSCYDRRVEEGSA"
     gene            5869..6162
                     /locus_tag="AC150V_07445"
     CDS             5869..6162
                     /locus_tag="AC150V_07445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3762794.1"
                     /translation="MRAPSTAACSIWSFEPGNVADGWQILGPTPPGGIHGGDEVSSPQ
                     VCFGVDDIASAVLQVRELGGEADEPVESVAGWYASCRDDQGSAFFLWSSSGRD"
     gene            6155..6997
                     /locus_tag="AC150V_07450"
     CDS             6155..6997
                     /locus_tag="AC150V_07450"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR3762795.1"
                     /translation="MTDTPEAPPQRTELDAAADAVRAHGGARPAVGVVLGSGLGGLAD
                     AIEDAVRIPYGEIPGWPASTAVGHAGTLVLGMFGGVPVAVMQGRAHLYEGVPPAKVVF
                     GVRVLGRLGIRTLVLTNAVGAIDTSLVPGQLVLIADHLNLQGASPLVGPNDDDLGPRF
                     PDLSDAYDSELRAAARGAAQRLGFELPERVYAAWLGPAFETPAEIRFLRAIGADLVGM
                     STVPEVLAARHMGVRCVAVSCVTNMAAGVLPEPIDHERVLEVGAEAQTRLTALLAELV
                     PSLA"
     gene            7027..8340
                     /gene="selA"
                     /locus_tag="AC150V_07455"
     CDS             7027..8340
                     /gene="selA"
                     /locus_tag="AC150V_07455"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HIR3762796.1"
                     /translation="MADSSLRRLPSVDRMLADPRLAGEPHELAVDACRAALDRARAEI
                     RAGRDPGNAVELALAELESARRPRLRRVLNASGVIVHTNLGRAPLSAAALDRVVEVGG
                     SYSNLEYDLAAGRRGSRQDHLADLLGRLTGAEAALVVNNTAAAVLLALAALAERREVL
                     VSRGELIEIGDGFRIPDVLVRSGARLVEVGTTNRTRASDYEGAIGPDTALLLRVHQSN
                     FRVVGFSEVPRLDELAAVAHTHRLPLVDDLGSGALYPLADEPTPAASLAGGADLVCFS
                     ADKLLGGPQAGIVVGRAELVERLRRHPLQRALRADKLTLAALEGTLVLALDPARARDE
                     IPVLRMLEEPIERVRKRAERLATRVGGDVEETVARVGGGALPLAQLESFACAVEEELA
                     ERLRVGDPPVVALVRDGRTLLDVRALADGDLDEVAEAVERARAAG"
     gene            8337..9287
                     /locus_tag="AC150V_07460"
     CDS             8337..9287
                     /locus_tag="AC150V_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR3762797.1"
                     /translation="MSDRVPEELLWDLLRGALTTRALGIVADLGVPESLASGPRRVEE
                     RARALDADCDALQRLLRALASDGVFEEERPGVFRNTPASELLRREGWGDFAHLFAGAW
                     HSAAGALEADGAPTFERLFGADYWSWLAARPDERAAFDRAMVEGTERRAERLEQLAWR
                     GDETVVDVGGGNGSLLVELLRRKPGLRGIVFDRPETVRDEERLGDRCVFVAGDFFEAV
                     PTGDLYVLSTVLHDWDDERAGAILRTIRAAAPAHGRLVALDSVVPPGNEPHGSKWLDL
                     LMLALFGGRERDEEQWRALLDGAGFEPLLLEDRLIEARCR"
     gene            9284..>9469
                     /locus_tag="AC150V_07465"
     CDS             9284..>9469
                     /locus_tag="AC150V_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR3762798.1"
                     /translation="MTVGTAGHIDHGKTWLVRALTGKDTDRLPEEQARGISIDLGYAP
                     LALPDGRRLSLVDVPGHE"
BASE COUNT         1267 a         3269 c         3405 g         1528 t
ORIGIN      
        1 cggaggcccg ccgacggtag tcgcgaccac cggcttgccc gcggcaaggc cctcgagggc
       61 tgcgatgccg aagggctcga cgaggctggg ctggcagagc acgtcggagg ctgcgatcca
      121 cgccgccgcg cgctcgtgcg ggacggcgcc ggcgagcaag acgcctggga tgcccacgag
      181 ctcgggtcgg agagggccgt cgccaacgaa ggcaagcgac ccctttccgt gcttcgcgaa
      241 ggcgcgggcg aggcgcacga cgttcttcct ctcggtcagt gagcccacgc agacgaacgc
      301 ggttccgtcc ggatgccagc ccacctcgtc gcgtgctttg tcctgctcca ggtcggcgaa
      361 cctggcattg tcgaccccgc agtcgacgac ggccgtcttc cccgtcgctt ctggcaccgc
      421 cgcctccagg cgcctccgca gccagtcgga aacggcgacg acggcggcgg agcgtcgcac
      481 tacgaggcgc gttgccgccc ggacggccga ccgggtgcgg gcgttctcca cgtcttgccc
      541 gtgcgcggtg accacgaccg gcgcccggcc ggccagcacc gcccagagcc cggtcgggac
      601 gaggaagtgc gcgtagacga catcgggccg gaaccgtcgt gaggacgcct ccgtctcgcg
      661 cagaagccgg gcgtagcggc cccgctcgcc gcggcggtcc aataccgcgc gctcgacctc
      721 gtgcccgcgt tcgacgagct ttcgctccag cgcagccacg aacgtcccga ggtcgggggc
      781 gtcggggccc gggtacatct gcgagacgag aaggatccgc accggggctc agcgggagcc
      841 ggtcgacgcg tccggcggca gctcagactc gctgcgcgtc atacgttccc cagagctcgc
      901 gaaggacacc gcagatctca ccgaccgtac agcgggcgcg aagcgcctcg cgcatcggga
      961 cgagcaggtt ctcgtcgccg acggcgacgc ggcgaacctc ggcgagcgcc cgctcggcgg
     1021 cgttcggatc acgctgtgcc cgcaccgccg ccgttcgctc gcgctgcagt cgctcggact
     1081 cggggtcgac ggtgagcagc tcgacgtgct ccgtcgcggt ctcggtgaat cggttgacgc
     1141 ccacgatgac ctgctcgccc gcttcgaccc gagcctggtg acggaaggac gcctcctcga
     1201 tctgttcctg cacgacgccc tgctcgatcg ccgcgactgc gccgccgcgc tcgtcgatct
     1261 cgtcgatgag cgcctgggcg agccgttcga gctcgtcggt cagggcctcg atgtagaacg
     1321 agccgccgag cggatcggcc gtgtcgatcg tgccggcctc gtccatcagg atctgctgcg
     1381 tccgcagggc gatccgggcc gcgtgctcgg tcggcagggc aagtgcctcg tcgaagctgt
     1441 tcgtatgcag cgactgcgcg ccgccgcaga tcgccgagag cgcctgcacc gccacgcgca
     1501 cgatgttgtt ctccggctgc tgcgcggtga gtgtcgagcc gccggtctgc gcgtgaaatc
     1561 gcagcgcctg tgccttgacg ttcgtggctc cgaaccgctc gcgcatgatt ctggcccaga
     1621 gtcggcgcgc cgcacggaac ttggcgacct cttggaagaa gttgttgtgg gcgttgaaga
     1681 agaaggagag ccgctcaccg aagcggtcgg gggacaggcc ggactgcacc gcggcgtcgc
     1741 agtacgcgat cccgttcgcg agcgtgaacg ccagctcctg cgccgcggtc gagccggctt
     1801 cccggatgtg gtagccggag atcgagatcg tgttccactg cgggatctcg gcctcgcagt
     1861 aggcgaagag atcggtggtc agccgcatcg acggccgagg cgggaagatg tagttcccgc
     1921 gcgcgacgta ctccttgagg atgtcgttct gcaccgtgcc ccgcagctgg tcgccgccga
     1981 ctccctgctc ttcggccacc agcgcgtaga gcagcagcag gagcgccgcg ggggcattga
     2041 tcgtcatcga cgtcgagacc tcgtcgaggg gtatgccgtc gagtagcacg tgcatgtcgt
     2101 cgatcgagtc gattgcgacg ccggtgcgcc cgacctctcc ctcggcgagg gggtcgtcgg
     2161 agtcgaggcc gagctgggta ggcaggtcga aggcgatcga cagtcccgtc tggccgcgct
     2221 cgaggaggta gcggtagcgc tcgttcgact cctccgccga cgcgaagccc gcgtactggc
     2281 ggatcgtcca ggggcgcccc cggtacatcg tcgagtaggg accgcgtgtg aacgggtact
     2341 cgcccggcgg ctcgagtccg cccgtcggcg cgtccgccgc gccgtagacg ggcttgatct
     2401 cgattcctga gtcggtctcg cgccggtcgg tcgccgccac gagcgaatcg tacgcgggac
     2461 ggcctacagg acgccgccgg cgcgctcgat cgcgtcgagg gcgatccgct tgccggcctc
     2521 gaggtggtcc ctgaccgcct gctgcgcaga gtccacgtcg cctgactcga acgcgtcgac
     2581 gatgcgcgcg tggccggtcg cgagctcctt gcttcgctcc acgcgtccgc ccctgatcac
     2641 ctgcatcatc aggttgacgg agagctcgcg gtagaagcgt gacagcaggt ggttctcggc
     2701 gaggtcgacc tggtactcgt gaaacgccgc gttcgcggtg tcccactcgt cgtgggacaa
     2761 cgccgccgcc gtcgcctcca tcaggctgcg gaatcgcgcc aggcgctcgg gcgccagtcg
     2821 ccccacggtg ttgagggccg cgtggagctc gagcgcgagc cggacgtcgt acccttcggc
     2881 gacgacctcg gccgtcacgg gcgtcacgaa gtacccgcgc cgcgccttga ccgtgagcag
     2941 tccctcggag acgagccggg tgagcgcgtg atgcacgggg ctgcgcgaga cgccgagctc
     3001 gtccgcgagc tcgtgcaggc tcagcttcgc accggggccg agctctctcg tcaggaggcg
     3061 cgcccgaagg ttctcgtaga cctgttcgtt caggttcgtc cgcgggagcg tgacggtcat
     3121 gggcagtttg taacatgtca gcccgcggca gggcgggcct ggctacgctg cgacggtgga
     3181 cgtcggtgtc aggctgttcg caggattgcg ggaccgcgcg ggggtcggac gggtggacgt
     3241 ccggctcccg gaaggcgcca cggtcgccga cgtgtggccc gccctcgacc tgggcgagga
     3301 gccgagcgga ctcctctacg ccgtcaaccg cgagtacgcc gaggcatcgg ctgcgctgag
     3361 cgacggcgac gaggtcgcag tcattccgcc cgtctcggga ggcgacttca ggctctccgc
     3421 ggagccgctc tcggtcgacc gggtcgtggc cgaggtggcg tccgaggccg ccggtgccgt
     3481 cgcgacgttc atcggcacga cccgtgcaag gtcgcgcgac cgggacgtcg tccgtctcga
     3541 gtacgaggcc tccgagggga tggccgaagc cgagctcgag cgcatcgccc agagcctgca
     3601 gcagcggtac gagctcgtcg gagtggcgat ccaccatcgc gtcggcgtcg tcggcgtcgg
     3661 cgagaccagc ctcgtcatcg ccgtctcggc tgcgcaccgc gccgacgccc tcgcggcctg
     3721 ccgcgacgcg atcgacgagc tcaaggtgtc ggtgccgctg tggaagaagg aggtctacgc
     3781 gggcggtgag gagtggatcg ggagcgaggc cgactatcag gccgagctgc tcgccgagcg
     3841 tgagtcggcg ggggggtagt cgtccctcgc atgtcgctgc cctgactgtg gcgaagcccc
     3901 gcagctgcgg ggcttcgccg gtccggtcgt tgacccgcta gcggttactc ctcgccgccg
     3961 atcgtggcgt cgtactgcgc ctcgcagtcg tcgtacaggt cggagcggaa aatcatgtcg
     4021 ggatagttct tatccccgtg tccttgggct cccggctggt ggccctgcgg gatggcgttc
     4081 ccgctgactt cgatcagcac gtacatgttc ttcgcggagg ctgtgccgtg gcacagaagg
     4141 actttgtcgg tgggttccgc ttgggcgccg acggtgacgg cgagagcggc gatcacggtg
     4201 gcggcgacga gcaacctgcg tgtgatcctc ctcattgatc gagtcctttc ggttggggcg
     4261 ttgctctcgt cgtcggcgcg gcgcagcgag tcagccaccc ctgggatgag tgaaggcccg
     4321 cgcgtcgcga cgacagcagg ctccctgcct ggcgaggtcg acgcgacagc aggcatcagt
     4381 acgctctatg agatgagcgc gagtcgatcg tgagcggcgc cgacccctac gagccccgta
     4441 gcgcgcccga gccggagcag tactcgccgc tcaagccgca gcgaggcgcg gtcgactggc
     4501 tcaagcgcct cgcggccccg ctcgtcgtgc tcggcggcct gctcgtcaag ttcggaggct
     4561 cgctcaagtt cctgggcatc ttcgtggccg tgggcggcta cgcattgatc tggggctggg
     4621 ccttcggtgt cggcttcgtg ctgctgatcc tggcgcacga gctcggacac ttcgtcgagg
     4681 ccaagcggca gggtctgagc ccctcgctgc cggtgttcgt gcccttcctc ggcgcattcg
     4741 tcgcgatcaa gggcgctccc ttcgacccgt ggcggaacgg gctcgtctcg atcgcggggc
     4801 cggtcgcggg cgcggccgcc gcgttcggcg ttctcgtcgc gggagacctc tacgactcgc
     4861 ggttcctgct cgcgctcgcg tacgtcggct tcttactgaa cctgatcaac ctggcgccct
     4921 ttgcgatcct cgacggcggc tacatcgccc gctcgtggcg gttgctccgc gccggcgccg
     4981 gagccatgac ggctgacgag gcacggcgga aggcctggct cctcggcggc gcctacgtcg
     5041 tcatcgcggg cctgctcgtc gtgggcatga tcgtggccca tgtcccgcag gaccgtctat
     5101 gacggacgat cgaccgcacg agaacgggct cgatcgcagg ctcctgcggc gaacccacga
     5161 gaccctcgcg agcgacgtct cgttgatcgc gagcgagttc ctcgccggct tccaggcggt
     5221 gcaggagatc gaccgtccgg ccgtgtcgat cttcggctcc gcgcgggtcg aggaggggac
     5281 acctgtctac gagctcgccc gcgaggtcgg ggccggcttc gcggcccagg ggttcgcggt
     5341 cgtcaccggc ggcggcccgg gggtcatgga aggcgccaac cgcggagcgc gcgacgcggg
     5401 cggcctgtcg gtcgggttca acatcgtcct gccccacgag cagagcatga acccgtacgt
     5461 cgggcacggt gtcaccttca agcacttcta cgcgcgcaag acgatgttcg tgaaggcggc
     5521 tgaggggttc gtcatcttcc ccggtgggtt cgggacgatg gacgagctgt acgagtcgct
     5581 cacactcatc cagacgaatg tgatcggcta cttcccggtc gtgctcttcg attccgacta
     5641 ctgggcggag atgcttcatt tcctgcgcgt cgagatgctc gaggacgggc tcgtctcgcc
     5701 cgaggatctc gagctcttgt acctaacgga ctcgcctgcc gaggccgtcg atctggtggt
     5761 gtcctgctac gaccggcgcg tggaggaggg ctccgcctga ggagaggggg agagaaagca
     5821 tggccggcga catcgtctac ttctggatac ccgctcccga ctcggaacgt gcgcgcgcct
     5881 tctacggcgg cctgttcgat ttggagtttc gagcccggga acgtcgccga cggctggcag
     5941 atactggggc cgacaccgcc cgggggcatc cacggcgggg acgaggtttc gagtccgcag
     6001 gtgtgcttcg gcgtcgacga catcgcctcg gccgtcttgc aggtgcgcga gctcgggggc
     6061 gaagccgacg agcccgtcga gtcggtcgcc ggctggtacg catcgtgtcg cgacgaccag
     6121 ggctctgcct ttttcctctg gtcgtccagc gggcgtgact gacacccccg aggctccgcc
     6181 tcagcgcacg gagctcgatg cggcggccga tgccgttcgc gcgcacggcg gcgctcggcc
     6241 ggcggtcggc gtcgtcctcg gcagcggtct cggtggcctt gcggacgcga tcgaggacgc
     6301 cgtccgcatc ccctacggcg agatccccgg ctggcccgcc tcgacggcag tcgggcacgc
     6361 ggggacgctc gttctcggga tgttcggcgg cgtcccggtg gcggtgatgc agggtcgcgc
     6421 tcacctctac gagggggttc cgccggcgaa ggtcgtcttc ggcgtacggg tgctcggccg
     6481 gctcggcatt cgcacgctcg tattgacgaa cgcggtcggc gcgatcgaca cctcgctcgt
     6541 gccggggcag ctcgttttga tcgcggatca cctcaacctc cagggagcat ctccgctcgt
     6601 cggcccgaac gacgacgacc tcggcccccg gttccccgac ctgagcgacg cctacgattc
     6661 ggagctccgt gccgccgcgc ggggagcggc ccaacgcctc ggcttcgagc taccggagcg
     6721 cgtgtacgcg gcctggctcg gcccagcgtt cgagacgccc gcggagattc gcttcctgcg
     6781 cgccatcggc gccgacctgg tcggcatgtc caccgtcccg gaggtgctgg cggcgcgcca
     6841 tatgggagtc cgttgcgttg cggtgtcctg cgtcacgaac atggccgcgg gcgtcctgcc
     6901 cgagccgatc gaccatgagc gggtgctcga ggtcggcgcg gaggcacaga cgcgcctgac
     6961 agccctgctc gccgagctcg tgccgtcgct cgcgtgagtc tcgaccggcc gtcctacact
     7021 gcgggcatgg ccgactcgtc tctgcggcgg ctgccctccg tcgacaggat gctcgccgac
     7081 ccgcgtctag ccggcgagcc gcacgagctc gcggtcgacg cctgtcgcgc tgcgctcgat
     7141 cgcgctcggg cggagatccg cgccggtcgc gatcccggca atgccgtcga gctggcactg
     7201 gccgagctgg aaagcgcacg ccgtccgcgc ctgcgccgcg tcttgaacgc atcgggcgtg
     7261 atcgtgcaca cgaatctcgg ccgcgcaccg ctgtcggcag ctgccctcga ccgtgtcgtc
     7321 gaggtgggcg gctcgtactc caatctggag tacgacctcg ccgccggaag gcgaggctcc
     7381 cgccaggacc acctggccga cctcctcggc cgcttgaccg gcgccgaagc ggcactcgtc
     7441 gtcaacaaca ccgccgcggc ggtgctcctc gcactggccg cgctcgccga gcgccgcgag
     7501 gtgctcgtgt cccgcggcga gctgatcgag atcggcgacg gctttcgcat tcccgacgtc
     7561 ctggtgcgct cgggggcgcg cctggtcgag gtgggaacga ccaatcgcac gcgggcgtcc
     7621 gactacgagg gtgcgatcgg ccccgacacg gccctgctgc tccgcgtcca ccagtcgaac
     7681 ttccgcgtgg tgggcttcag cgaagtgccc cggctggacg agctggcggc cgtggcccac
     7741 acgcatcgac tgccactcgt ggacgatctc ggctcgggcg cgctgtatcc cctcgctgac
     7801 gagcccaccc cggctgcgag cctcgccggc ggggccgatc tcgtctgctt ctccgccgac
     7861 aagctcctcg gcggcccgca ggccggaatc gtggtcgggc gggccgagct cgtggagcgc
     7921 ttgcgccgac acccgctgca gcgcgctctt cgcgccgaca agctcacgct cgcagcgctc
     7981 gaggggacgc ttgtgctcgc gctcgacccg gcccgggcac gtgacgagat ccctgtgctg
     8041 cgcatgctcg aagagccgat cgaaagggtg cggaagcggg cggagcgcct ggcgacgcgg
     8101 gtgggcggcg acgtcgagga gacggtcgcg cgggtcggtg gcggtgcgct cccgctcgcg
     8161 cagctcgaga gcttcgcctg tgccgtcgag gaggagctcg cggagcggct gcgcgttgga
     8221 gacccgcccg tggtggccct cgtccgcgac ggccgcacgt tgctcgacgt gcgcgcgctc
     8281 gccgacggcg acctcgacga ggtggcagag gccgtcgaac gcgcccgcgc cgccggatga
     8341 gcgaccgcgt ccccgaggag ctcctctggg atctgctcag gggcgcactc acgacccgcg
     8401 cgctcggcat cgtcgccgat ctcggcgttc ccgaatcact cgcctcgggt ccgcgccgtg
     8461 tcgaggagcg ggctcgggcg ctcgacgccg actgcgacgc gctccagcgg ctgctgcgcg
     8521 cgctcgccag cgacggtgtc ttcgaggagg agaggccggg tgtcttccgc aacacgccgg
     8581 catcggagct tctgcgccgg gaggggtggg gcgacttcgc ccatctcttc gccggtgcat
     8641 ggcatagcgc cgcaggagcg ctcgaggccg atggtgcgcc cacgttcgaa cggctcttcg
     8701 gcgctgatta ttggagctgg ctcgcggcac ggcccgacga gcgtgctgca ttcgaccggg
     8761 cgatggtcga gggcaccgag cgacgagcag agcggctgga acaacttgcg tggcgcggcg
     8821 acgagacggt ggtggacgtc gggggcggca acggctcgct gctcgtcgag ctgctgcgcc
     8881 ggaagcccgg gctccgcgga atcgtctttg accgccccga gaccgtgcgc gacgaggaac
     8941 ggctcggcga ccggtgtgtc ttcgtggcgg gcgacttctt cgaggccgtc ccgaccggcg
     9001 acctgtacgt cctgtcgacc gtcctgcacg actgggacga cgagcgcgca ggcgcgatcc
     9061 tgaggacgat tcgggccgct gcgcctgccc acggcaggct tgtcgccctt gactccgtgg
     9121 tgccgcccgg caacgagccg cacggatcga agtggctcga cctcctgatg ctcgcgctct
     9181 tcgggggccg tgagcgcgac gaagagcagt ggcgggcact tctcgacggt gccggcttcg
     9241 agccgctgct cctggaggac agactcatcg aggcgcggtg ccgctgaccg tcggcaccgc
     9301 cggacacatc gaccacggca agacgtggct cgtgcgcgcg ctcacgggca aggacaccga
     9361 ccggctgccc gaggagcagg cgagggggat ctcgatcgac ctcgggtatg cgcccctcgc
     9421 actgccggac ggccgccggc tctctctcgt cgacgtgccc gggcacgag
//
LOCUS       DBNGNK010000254         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1979842,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000254 DBNGNK010000000
VERSION     DBNGNK010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1979842"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /gene="sufD"
                     /locus_tag="AC150V_07470"
     CDS             <1..699
                     /gene="sufD"
                     /locus_tag="AC150V_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR3762799.1"
                     /translation="SASKELEGYVNAAVEIFVKDAAKVEYISVQNLSRRSWLFSSSHA
                     AVHRDAELDWVAGGLGSAKGKVRIQNDLAGPGATSRVTGAYFADGSQHLDFDTFQEHI
                     APHTTSDFAFKGALRDTATAVWRGMIRVEQDAQKTNAYQENRNLLLSKTAHADSIPGL
                     EILANDVRCTHGATLGQVDRDQLFYLMARGLPRAEAERLIVRGFYQDVLDRIELEPVR
                     EALGAALEARIPQA"
     gene            745..1086
                     /locus_tag="AC150V_07475"
     CDS             745..1086
                     /locus_tag="AC150V_07475"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR3762800.1"
                     /translation="MAVTPRPVMMDVTRRGGDQMHTYVGLIKLSSEAREHLSDAPSYL
                     EKVKEIIEGEGGTLENVYAIMGPWDFLAITTYPENEAAFRVLAKIGALENVTTETFPA
                     ERVEVFLEALT"
     gene            complement(1146..>2664)
                     /gene="ettA"
                     /locus_tag="AC150V_07480"
     CDS             complement(1146..>2664)
                     /gene="ettA"
                     /locus_tag="AC150V_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR3762801.1"
                     /translation="GQEEPSNGIAELAPQATVGLLEQEPVLDAGKDVRGNVEDGVRHL
                     RDLLDRFNAISAEFADPDADYDALLAEQARVQDDIDRHDAWTLDAALDRAMDALRLPD
                     GDRDVTTLSGGERRRVALCRLLLAAPGLLLLDEPTNHLDAESVAWLERFLADYQGTVV
                     AVTHDRYFLDNVAGWILELDRGRGIPYEGNYSGWLEQKQARLAAEEKQESARRRALAR
                     ELEWVRSSPKARHAKSKARLGAYERLLEEERSVKLDRVEIHIPAGPRLGDVVVQADDV
                     RKGYDDRLLIDALSFSLPPGGIVGVIGPNGAGKTTLFRMIAGEEQPDGGELRIGDTVE
                     LAYVDQSRASLDPANSVWKEISGGLDTIELGAREVSSRQYVSWFNFKGPDQQKRVSDL
                     SGGERNRLHLAKLLRSGGNLLLLDEPTNDLDVDTLRALEDALLGFAGCAVVISHDRWF
                     LDRIATHVLAFEGESAVTWFEGSFSEYEDWVRETRGVEALEPHRITYKPLVRPGA"
BASE COUNT          430 a          886 c          922 g          426 t
ORIGIN      
        1 tccgcgtcga aggagctcga gggctacgtg aacgcagcgg tcgagatctt cgtgaaggac
       61 gccgccaagg tcgagtacat cagcgttcag aacctgtcgc gacggagctg gctgttcagc
      121 tcctcacatg cggccgtcca ccgcgacgcc gagctggact gggtcgcggg cggcctcggc
      181 tcggccaaag gaaaagtgag gatccagaac gacctcgccg gccccggcgc gacgtcgcgg
      241 gtcacgggcg cttacttcgc agacgggagt cagcacctgg acttcgacac gttccaggag
      301 cacattgcgc cccacacgac gtccgacttc gcgttcaagg gcgctctccg cgacacggcg
      361 acggcggtct ggcgcggcat gatccgggtc gagcaggacg cccagaagac caacgcgtac
      421 caggagaacc gcaatctgct cctgtccaag acggcccacg ccgactcgat ccccgggctc
      481 gagatcctcg ccaacgacgt tcgctgcaca cacggagcga ctctcggtca ggtcgaccga
      541 gatcagctct tctacctcat ggcccgcgga ctgccgaggg cggaggcgga gcgactgatc
      601 gtgcgcggct tctaccagga cgtcctcgat cggatcgagc tggagcccgt gcgcgaggcg
      661 ctcggggcag cgctcgaggc ccgcatcccc caggcctagt ccggaaacag cgcgtccgtt
      721 ctgccgatat cagcggtttt gccgatggca gtcacgccgc ggcctgtcat gatggacgtc
      781 acccggcgag gaggtgacca gatgcacacg tacgtcgggt tgatcaagct cagctcggag
      841 gctcgggagc acctgagcga tgcgcctagc tacctcgaga aggtcaagga gatcatcgag
      901 ggcgagggag gcacgctcga gaacgtgtac gcgatcatgg gtccttggga cttcctggcg
      961 atcacgacgt acccggagaa cgaggccgcg tttcgcgtcc tggccaagat cggggcgctc
     1021 gagaacgtga cgaccgagac cttcccggct gaacgggtcg aggtcttcct ggaagcactg
     1081 acgtagcgcg agtcgagcgc ggaggactgt ccgcacgggc tctctttcgc gctgcgcgga
     1141 gtggtctagg cgcccgggcg caccagcggc ttgtacgtga tgcggtgcgg ctcgagcgcc
     1201 tcgacacccc tcgtctctcg cacccagtcc tcgtactcgc tgaagctccc ctcgaaccag
     1261 gtcacggccg attcgccctc gaaggcgagg acgtgcgtcg cgatgcggtc gaggaaccag
     1321 cggtcgtgcg agatgacgac ggcgcagccg gcgaagccga gcagcgcatc ctcgagtgcc
     1381 cgcagcgtgt cgacgtcgag atcgttcgtg ggctcgtcca gcaagagcag gttccctccg
     1441 gagcgcagca gcttggccag gtgcagccgg ttccgctcgc cgcccgagag atcgctgacg
     1501 cgtttctgct gatccggccc cttgaagttg aaccacgaga cgtactgacg cgagctcacc
     1561 tcgcgggccc cgagctcgat cgtgtcgagc ccgcccgaga tctccttcca cacgctgttc
     1621 gccggatcga gactggcgcg gctctgatcc acgtaggcga gctcgacggt gtcgccgatc
     1681 cggagctcgc cgccgtcggg ctgctcttct ccggcgatca tgcggaaaag agtggtcttg
     1741 ccggctccgt tgggcccgat cacgccgacg atcccgcccg gggggagcga gaaggagagc
     1801 gcgtcgatga gcaggcggtc gtcgtacccc ttgcgcacgt cgtccgcctg gacgaccacg
     1861 tctccgaggc gtggtccggc gggaatatgg atctcgaccc ggtcgagctt cacggagcgc
     1921 tcctcctcga gcaggcgctc gtaggcgccg agccgggctt tcgacttcgc atggcgggct
     1981 ttcggagacg agcgcaccca ctcgagttcg cgggcgagcg cgcggcgccg ggccgattcc
     2041 tgcttctctt cggcggcgag acgggcctgc ttctgctcca gccagccgga gtagttgccc
     2101 tcgtacggga tgccgcgccc acggtcgagc tcgagaatcc agcccgcgac gttgtccaga
     2161 aaatagcggt cgtgggtgac cgccacgacg gtgccctggt agtcggcgag gaaccgttcg
     2221 agccaggcca cggactccgc gtcgaggtgg ttggtcggct cgtcgagcag cagcagaccc
     2281 ggcgccgcga gcaggagccg gcagagggcc acgcggcgac gctctccgcc cgagagcgtc
     2341 gtgacgtcgc ggtcgccgtc gggaagccgg agcgcgtcca tcgctcggtc gagcgcggcg
     2401 tcgagggtcc atgcgtcgtg gcggtcgatg tcgtcctgaa cgcgcgcctg ctcggcgagc
     2461 agggcgtcgt agtcggcgtc gggatcggca aactccgccg agatcgcgtt gaagcggtcg
     2521 agaaggtcgc ggaggtggcg gacgccgtcc tcgacgttgc cgcgcacgtc cttgcctgcg
     2581 tcgagcacgg gttcctgctc gagcaggccg accgtcgctt gcggcgccag ctcggcgatc
     2641 ccgttcgagg gctcttcctg accg
//
LOCUS       DBNGNK010000255         3381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_905813,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000255 DBNGNK010000000
VERSION     DBNGNK010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_905813"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="AC150V_07485"
     CDS             <1..635
                     /locus_tag="AC150V_07485"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762802.1"
                     /translation="LVSMSGPVLADRVGPKKVVQAGFLTLALSVVILLGAIEPTLDGF
                     GFGVALAVLGVGMGLIASQLGNIVQSAVGEKDRSEAGGLQTTAQQLGSSLGTALIGAI
                     VLSGLTATFVTNVAEDERISAEVKTSLEARSGVVFVNSGEVEQAATDAGLPGQEVDAI
                     VHAYESSQLTALKVGLLVAGFIALAALPLTRSLPRRFGEESEAAGTAPAR"
     gene            644..1357
                     /locus_tag="AC150V_07490"
     CDS             644..1357
                     /locus_tag="AC150V_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762803.1"
                     /translation="MTDERTEWLLEFAAQLDVRDGAIAAEIDQVAGLARRAEEIGERA
                     GAIADRLAALPGEQAAAEQRETDARAALARARDEEEAARESVARLEGKRRKREELDAA
                     SRDLARAEERVRDAIARVERTEAARAAVRTEERGLQLEAAGLAAAAPEVADGIELVPR
                     VSESGKTKPGRELGELVEWAARARAALFVVRGTLVSERERIALEAKAVGEAALGEDFG
                     GATVAQVRRRIERELAAGS"
     gene            complement(1364..2263)
                     /locus_tag="AC150V_07495"
     CDS             complement(1364..2263)
                     /locus_tag="AC150V_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762804.1"
                     /translation="MGDSRRRTQILIRGSLLLFVVAWLFSADLRGVIPFWLPFAVLLA
                     TEAEFLFRGLRESPQPQPASAEGVLERRSPGVDDADLGWLDVELGVPAPPRERRRSTP
                     RRLAYAAGAVVAVGLFVLALRSDESANWSSISEADRQRAQTRFSREASAIAGKPVQIL
                     CDESYAFTGAATDAAGVAFRNRGLALLAPDICRTLYDFAFAGEEPRRDQTGWAIVVLA
                     HEATHLRGIRDEGLTECYALQEGVALGQRLGLDRAEAAELMGRQLDRSLSARDLSRLD
                     YRPPEECKEGGKLDLRPDDPVFP"
     gene            2313..3287
                     /gene="speB"
                     /locus_tag="AC150V_07500"
     CDS             2313..3287
                     /gene="speB"
                     /locus_tag="AC150V_07500"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR3762805.1"
                     /translation="MVDPLRRWKELGLPDKPDFAGLLTYGGLLFTDDPAELRDVDVVV
                     IGAPTDDLVSDRPGARFGPRAIRAASCPPGPHLEAGVDAFEELAVVDFGDAAVVPADP
                     VRTHAAIERVVGEAVDAGTIPVVLGGDHSITEPELRAVARRHGPVGLVHFDTHTDTGT
                     EVFDVEISHGTSLYRVVRDGLVDPARYVQIGLRGYWPGEAEFAWQAEQGITSFFMHDV
                     RTLGIEEIVGRTVGAVGDGPVFLTVDVDVLDPVYAPGTGTPEPGGMTSADLLWAVRAI
                     GERLDLVGVDVVEVAPTGIGSADVTALVADRIVREALTGVALRRRSRA"
BASE COUNT          509 a         1135 c         1243 g          494 t
ORIGIN      
        1 tgctcgtctc gatgagcggt cccgtgttgg ccgatcgtgt cgggccgaag aaggtcgtcc
       61 aggcaggctt cctgacactc gcgctttcgg tcgtcattct gctcggcgcg atcgagccga
      121 ctctcgacgg cttcggattc ggcgtcgctc tggcggtgct cggagtcgga atgggcttga
      181 tcgcgtcgca gctcggcaac atcgtccagt cggcggtggg tgagaaggac cggagcgagg
      241 ccggtggtct gcagacgacg gcgcagcagc tcgggagctc gctcgggaca gccctgatcg
      301 gggcaatcgt gctctccgga ctgacggcga ccttcgtcac caacgtggcc gaggacgagc
      361 gcatctcggc cgaggtcaag acgtcactcg aggctcgcag cggcgtcgtc ttcgtcaact
      421 ccggcgaggt cgagcaggcc gcgaccgacg ccggcttgcc cgggcaggag gtggacgcga
      481 tcgtccacgc gtacgagagc tcgcagctga ccgctctcaa ggtcggccta ctcgtcgctg
      541 ggttcattgc cctcgcggcc ttgcctctaa ccagaagcct cccgcgacgc ttcggcgagg
      601 agtccgaggc agcagggact gctcccgcgc gataatcgag agcgtgacgg acgagcggac
      661 ggagtggctg ctcgagtttg cagcgcagct ggacgtgcgg gacggcgcga tcgcggcgga
      721 gatcgaccag gtcgcgggcc tggcgcggcg cgccgaggag atcggcgagc gcgcgggggc
      781 gatcgccgat cgtctcgcag ctctgcctgg ggagcaggcc gctgcagagc agcgcgagac
      841 ggacgcgcgg gcggcactcg cccgcgcgcg cgacgaggaa gaggccgccc gtgagtccgt
      901 cgccaggttg gagggcaagc gcagaaagcg cgaagagctc gacgcggcct cgcgcgacct
      961 cgcgcgcgcc gaggagcgtg tccgcgacgc tatcgcgcgc gtcgaacgga ccgaggcggc
     1021 tcgggcagca gtccggacag aggaacgcgg gttgcagctc gaagcggcgg ggcttgctgc
     1081 ggcggctccg gaagtggccg acggaatcga gctcgttcct cgcgtctccg aatccggaaa
     1141 gacgaaaccg gggagggagc tcggcgagct ggtggaatgg gcggcgcgtg cgagggctgc
     1201 gctcttcgtc gtgcgcggga cgctggtcag cgagcgtgag cggatcgcgc tcgaggcgaa
     1261 ggccgtcggc gaagccgctc tcggcgagga tttcggcggg gccactgtcg cgcaggtccg
     1321 ccgcaggatc gagcgggagc tcgcagcggg gagctgagcc cgttcagggg aagaccgggt
     1381 cgtcgggacg cagatccagc ttgcccccct ccttgcactc ctccggaggt ctgtagtcga
     1441 ggcgtgagag atctctcgcc gacaggctgc ggtcgagctg ccggcccatg agctccgccg
     1501 cctccgcgcg gtcgagcccg aggcgctggc cgagcgcgac cccttcctgg agcgcgtaac
     1561 actcggtcag gccctcgtct cgaatgccgc ggaggtgggt cgcctcgtgt gccagcacga
     1621 cgatcgccca tcccgtctgg tcgcgccgcg gctcctcgcc cgcaaaggcg aagtcataga
     1681 gcgttcggca gatgtcaggc gccagcaggg cgagcccgcg gttgcggaag gcgactcccg
     1741 cagcgtcggt tgcggccccg gtgaaggcat acgactcgtc gcagaggatc tgcaccggct
     1801 tgcccgcgat cgcgctcgct tcgcgggaga aacgcgtctg tgcccgctgg cgatccgcct
     1861 cggagatcga cgaccagttc gcgctctcgt cgctgcgcag agcgaggacg aagagcccga
     1921 cagcgaccac ggctcccgcc gcgtatgcga gccggcgcgg agttgagcgt cgtcgttcgc
     1981 gtggaggcgc cggcaccccg agctcgacgt cgagccagcc gaggtccgcg tcgtccacgc
     2041 cggggctgcg ccgctccagg acaccctcgg cgctcgcggg ttgaggctgc ggagactcac
     2101 gcaggccgcg aaagaggaac tcggcctcgg tcgcgaggag cacggcgaac gggagccaga
     2161 acggaatcac tccgcgcagg tcggccgaga agagccaggc cacgacgaag aggaggagcg
     2221 acccccggat cagaatctgc gtccggcggc gcgagtcgcc catacagcca cagtagccag
     2281 ccccctcggg cacgccgtag gatcggcgca cggtggtcga cccgctcagg cgatggaagg
     2341 aactcggtct gcccgacaaa ccggactttg ccgggctcct cacctacggc ggcctgctgt
     2401 tcaccgacga tccggcagaa ctgcgagacg tcgacgtggt cgtgatcggt gcgccgaccg
     2461 acgatctcgt ctccgaccgc cctggagcac gtttcggccc ccgtgcgatc cgcgcggcga
     2521 gctgcccacc gggacctcac ctcgaggccg gggtcgacgc gttcgaggag ctcgccgtcg
     2581 tcgacttcgg cgacgccgcg gtcgtgcctg cagatcccgt ccggacacac gctgcgatcg
     2641 agcgtgtggt gggcgaagcg gtggacgccg gaacgattcc cgtcgttctc ggcggagacc
     2701 attcgatcac cgagccggag ctccgagctg tcgcccggcg gcacggtccg gtcgggctcg
     2761 tccacttcga cacccacacc gacacgggca cagaggtatt cgacgtcgag atctcccacg
     2821 ggacgtctct ctaccgggtc gtgcgtgacg gcttggtaga tccggcgcgg tacgtccaga
     2881 tagggctccg cggttactgg cccggcgagg ccgagttcgc ctggcaggcc gagcagggca
     2941 tcacgagctt tttcatgcac gacgtccgta cgctcggcat cgaggagatc gtcggccgca
     3001 cggtcggcgc ggtgggcgac ggtcctgtct tcctcaccgt cgacgtcgac gttctcgacc
     3061 ccgtctacgc gcccgggacg ggaacgccgg agccgggtgg aatgacgtct gccgacctgc
     3121 tctgggccgt gcgggccatc ggcgaacggc tcgatctcgt cggcgtcgac gtcgtcgagg
     3181 tcgctcccac tgggatcggc tccgccgacg tgaccgcgct ggtcgccgac cggatcgtgc
     3241 gcgaagcgct gacgggcgtc gcgctgcgcc gtcgctcgcg cgcctgatcg cctggcgact
     3301 gcacgccgcg aaagggccga gtcgggtcct cagaggaccg gccgtggtcg tcccggccac
     3361 ggctcgacga gccgggtggc c
//
LOCUS       DBNGNK010000256        18768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_46972,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000256 DBNGNK010000000
VERSION     DBNGNK010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 18768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18768
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_46972"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1547)
                     /locus_tag="AC150V_07505"
     CDS             complement(<1..1547)
                     /locus_tag="AC150V_07505"
                     /inference="COORDINATES: protein motif:HMM:NF039776.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HIR3762806.1"
                     /translation="MLVRLRAVVFLVLAASIFALGAAAQDGDVVFRLTNFLDLNKRGA
                     NKWPLPTEVPPLQQGYWDLRQLKNCRVPLSLLTKDAEANDVLPKGPEGAVLSRADVRA
                     QIDAAVAVWNAVRPSVLRIDLKHDAGADWPGNADPVALDQANVFLFARRGGPDEPGVT
                     VPQDPERRFKRRGVQQEIVDSDVALHRLFIRWALDAADHDLTKRPVVVDLQTTAIHEL
                     GHFVGLGHTSEDATEADAALRDPIMFFAVPTTARRELHASDKAGVNFLYTPDLGDADR
                     RYDGQTQTHGPECDRTLNDVPLLDPGKGAIHLLGAPPYEFEKLGDGVVSGECDPRQVD
                     DDSDDGVEVKADLRPGGTLELKVKIRVHKGKDGTGDGRYEPKNKNKNKNENKTKRAAY
                     LNAWADWQDDGTWDDKKDKIIGTDVGKTETQPHGTQAFTSDEDVEYTVAVPRNVSREA
                     LKKGLWLRFRLDYAEDAGKVKQKWSSPDLAGPEGMAAFGEVEDWVVTPEPPRKSSDPE
                     SGLPGGN"
     gene            complement(1603..2538)
                     /locus_tag="AC150V_07510"
     CDS             complement(1603..2538)
                     /locus_tag="AC150V_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762807.1"
                     /translation="MRAAATIAFLAAVALAVASVVSAAPEAGPRVAGCPVFPSDNFWN
                     QRVDRFPAAPTSTAIVASIGLDEHLHADFGSGLWQGGPIGIPITVVGRRVKGSRVRFQ
                     YSAESDRGPYPIPRAVKIEGGRRSEGDRHAIIVDRERCRLYELFALYPAGSGWRAASG
                     AVWDLRSNRLRPKGWTSADAAGLPILPGLARYEEVARGRIAHALRFTVSRTRRAYVWP
                     ARHFASSSSDASLPPMGLRLRLKRNFDISGFPPQARVILRALKEYGMVVADNGSDWFV
                     SGAPHPRWDNDDLHSLHRVPGSAFEVVDARALRKS"
     gene            complement(2597..3160)
                     /locus_tag="AC150V_07515"
     CDS             complement(2597..3160)
                     /locus_tag="AC150V_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007574892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HIR3762808.1"
                     /translation="MKTPDALHFTGDEEADALIAREPLALLIGFALDQQVPVQTAFAG
                     PLKLERRLGTLAAGEIAATDPDELEAVFREKPAIHRFPGNMARRVRELCAVVEGEYGG
                     HAERVWTEAADANDLRQRIGALPGFGEMKIKALGSVLAQRFGVEAAEPLVPDHPTLGD
                     VDSVDALEAYQAKKRAYKAQRRSAQGS"
     gene            complement(3157..3717)
                     /locus_tag="AC150V_07520"
     CDS             complement(3157..3717)
                     /locus_tag="AC150V_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762809.1"
                     /translation="MFVSARRRAVLLVAVLALTAVGVGSAALLAHLTAPAAFAQDTAD
                     TTDTTTETTDTTTTTDTTDTNGAGGSGGGDSGVPGWAWALVGAGIVLLFLLVFALGRR
                     GRGEARMAGGTSPVLTAKDRQARFGPALNAYQRDGWAVESRGADQAVLTRDRIRKRVW
                     VDEYGELQEELLAREPDGFDEQGEGR"
     gene            3775..8055
                     /locus_tag="AC150V_07525"
     CDS             3775..8055
                     /locus_tag="AC150V_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3762810.1"
                     /translation="MEPLAALGANTREWFARAFAAPTPAQELGWPAIASGAHVLIQAP
                     TGSGKTLAAFLVGIDRLNDTPGDGLRLLYVSPLKALNYDVERNLRGPLAGLRSELSVG
                     VRTGDTPQRERQQMLRRPPDILITTPESLFLLLTSQARQTLRGIETVILDEVHAVAGT
                     KRGSHLALSLERLEELADRPFQRIGLSATQRPLEEIGRFVSGGRPIELVDAGTRKELD
                     LEVVVPVEDMRELGSRQALSQPVVPDGVAMGSGYEPSARSIWPSIYPAVLDLVRQHRS
                     TIVFVNNRRLAERLAIRLNELAGEDLARAHHGSLAREQRVEIEELLKRGGIPCLVATS
                     SLELGIDMGAVDLVVQVESPKSVARGLQRVGRAGHELGSVSKGRLFPKFRADLLESAV
                     VAKAMREGAIEETSIPRNPLDVLAQQIVAICADAEIGVDDLYRVVRGAYPFADLSRQQ
                     LENVLDMLAGRYPSDEFAALRPRIVWDRTGGVIRGRTGARRLAVTNAGTIPDRGLYGV
                     FQLDGGGRVGELDEEMVYEAREGQTFLLGASTWRIEEITRDRVLVSPAPGVPGAVPFW
                     KGEGVGRPFELGERIGRASRELVSLTDDRALGRLGEDYALEPRAAQNLLTYLREQEAA
                     TRAVPSDRTVVVERFRDEIGDWRVCILTPFGGRVHAPWSMALAARLREALGLEVQSLW
                     SDDGIALHLPEADSPPPIDDLLVAPDEIEDLVVQEVGQTALFGARFRENAARSLLIPR
                     RRPGQRTPLWQQRLKAQSLLQVARRYGSFPVVLETYRECLQDVFDLPALKRILERLQT
                     RELDLVDVETPSASPFASSLLFDYVATYMYEDDTPAAERRAQALSLDRELLRELLGAE
                     ELRELIDPGALAEVEGSLRLSPRNPDELHDLLRRAGELLEGEFDAGFAETLLRERRAI
                     RVPIGGRNGLAAAEDAGRYRDAFGAMPPGGLPDAFLEPVPDALESVVRRFARTSGPFT
                     TAEVAARYGLSEEEADALLGGLERADLLVRGELRPGGVEREWCDPDVLRRIRRASLAV
                     LRREVEPAEQTALGRFLPAWHGIGRRAELREALVPLQALPLPVSLWETDVLPQRVPGY
                     RPEQLDALCASGELVWVGAGLDRVAVYFRDDASVLGPPAGAESPAAPAHDALRLVLTR
                     GAEFWPDLVAASGLESDEALAALWELVWAGEVTNDAWSPLRAARRYGTPVAEQRPRRF
                     SRSRARRSTATQGRWTRVAPVFGELPDRRALSELLLERQGIVTRDGVRGEGIRGGFGA
                     VYGELRVLETVGVCRRGYFVEGLGGAQFALAGAVERLRELRAEDDGDALVLAAADPAQ
                     PYGAALPWPRRAGARAARVAGAHVVLLGGDAVLFVERGGRSLVPLRDPDETWLRPALA
                     ALVARVKVGGKRLAVERFDGVPVTDSDVLPLLVEAGFLLGPRRAVLRP"
     gene            8052..9539
                     /locus_tag="AC150V_07530"
     CDS             8052..9539
                     /locus_tag="AC150V_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR3762811.1"
                     /translation="MTRTVWLLGDQLDRSGAALRDAEANSTTVLLVESAAKLAEKRWH
                     RQRLHLVLAAMRRFADELREDGFEVDYRHAASLESGVRDHLAEREPDELVIEEPMSWD
                     GLRLAKQLGCRIVRSERFLCHYDEFAAWAQGRKRLVLEHFYRDQRVRHGYLMDGDEPA
                     GGRWNFDADNRQPPPRDEVEWPRPPLEALDALDHEVLEALPAAVWGEAAVGLWPTNRN
                     HALALLSHFVDEVLPGFGPYEDAMLAEEWRLRHSLLSPALNLGLLHPREVCDAVERAY
                     RAGAVPIASAEGFIRQVLGWREYVWGMYWAAMPSYRDENELAAEEELPPLYRGGPTRM
                     HCASVTIRGVEERAWAHHIQRLMVLGNLSLLAGVRPRELVEWMWASFVDGAEWVMVPN
                     VIGMALHADGGRMATKPYAAGGAYINRMSDYCRACEFDPKQRVGEDACPFTTLYWDFL
                     DRQRDRLAANHRLGRQLANLDRLGDLDAVRARAQDVRRRLAAGEL"
     gene            complement(9575..10444)
                     /locus_tag="AC150V_07535"
     CDS             complement(9575..10444)
                     /locus_tag="AC150V_07535"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR3762812.1"
                     /translation="MANLLNISALSRRTGVAPDTLRKWEQRYGVLHPTRTTGGQRRYT
                     EQDVARVEWLRDRLREGWRIGEASRVLEETHRPTAADPAGLLEALIQATRDSSSGEIS
                     ATLDQTFAVLPIEPAIAKVIAPALRWVGDEWEVGRISVAQEHLLSGQVRARLDSFLGD
                     LRGGIRGTAVLACAPGEQHDLGLLMLAVLLRADGWRVEYLGQDTPVEEAVGFAELLDA
                     GLLCLSASRDEAMDAIRDGLADLPHEPRAALALGGPGVTSKAAKALGASYANGDFEAN
                     VARLRRYATGIRS"
     gene            10490..11242
                     /locus_tag="AC150V_07540"
     CDS             10490..11242
                     /locus_tag="AC150V_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR3762813.1"
                     /translation="MPEGDSVHRAAARLQVLVGERLEAEAPHPRGAVTGVAAAVDGKV
                     LEAVEARGKNLLLRFEGGVTVRSHLRMSGRWRVDRRGGRRTGRPWLVLRGREWEAVQW
                     NGPVLTLDGREAIRRLGPDLLVATTDPHDVVPRLRQSDPRRLLGEVLVDQRLVAGIGN
                     MWLAESLWHARASPWLSLGEASDAELVAALAHASNGMRDAAAGPRPLRKVYRRAGRPC
                     PRCGATLRSRGLGDANRTAYWCPRCQRGPDVS"
     gene            11299..12522
                     /locus_tag="AC150V_07545"
     CDS             11299..12522
                     /locus_tag="AC150V_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762814.1"
                     /translation="MPLRAPHLHLALRSFALGAFAYLLRELDDGGEELPFAFEEHPQR
                     DGPALYEYRPLVRDFIESRADRLRTRDDAILAVDELRREPTAAIFARAHAGATPSEDE
                     ALFRTVLLALLVASAESCGGFDWDDAAFEHAYAELEHSLFGEHRAYRAVAPLTGISVG
                     TRVELSAAIRMRAAADGELNRHWPEARGLLPEDFGREADRYCVLELRADLGPADEPPD
                     APAEIADAVSAVRLATAAPLAAGPVLFETLDGRPFGIRPVLPIAATEPPGDPGRLDEF
                     RGTLAVELLAQLGSADGDPALSEALDRWELSLFQHDPFRSEQLRGSFTALLGETWALR
                     AAVLLEDEPDGRGALHQQLAALAEGATATPAATDAARRMLVAALRHGDRHELVGELDG
                     RLLGLRERATLGLAV"
     gene            12591..12797
                     /locus_tag="AC150V_07550"
     CDS             12591..12797
                     /locus_tag="AC150V_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762815.1"
                     /translation="MDAARRVLSRLAQIEALERADAPPIDLLSALASLAEEAEDWAAA
                     EGGEEARAAVARLRAALTRDMIAM"
     gene            12794..13744
                     /locus_tag="AC150V_07555"
     CDS             12794..13744
                     /locus_tag="AC150V_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3762816.1"
                     /translation="MITHWDDVDGIRRTEGHIAADWHPLTGASSVSVGVRRIKVDPGM
                     WSTPLHVEGSEEEIFYVLGGTGTSVQWDGEHTESFELASGDCLVHLALENAHTLRAGD
                     AGIDVLAFGQRHYAPGVTVLPRAEAAWLGASWGRVGGADDHPWKREAAQGPPEVSGLS
                     PRPDRIVNVEDVQPIEHVGATVGRRIRDLGRAAGSERTGLRHCEVLPGMLNVPPHCHS
                     AEEEIFVVLAGEGVLLLWPTGADASDEQPVTAGHTISRPAGTGVAHAFRGGENGMSLL
                     MYGTRESGDVCYYPRSNKLSFRGLGVIARLELLDYWEGED"
     gene            13781..14716
                     /locus_tag="AC150V_07560"
     CDS             13781..14716
                     /locus_tag="AC150V_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR3762817.1"
                     /translation="MGLLRLLPRPASRLDEVEPDADSTDAPDTPASVSPPEMIFEPDL
                     SSEGRSQVRVLALANQKGGVAKTTTALNLGVAFREMGHRVLLIDLDPQGNLTMSQGMN
                     PDVIERSMFDVLVHRIPISDVIEHTEVDLAVASIDLAGAELALSSMIGRERSLEKALV
                     EVRDRYDFILIDTPPSLGLLTINAFVGATGVIVPVQCEYLSLRGLVQLENTLAMVREN
                     LNPHVGVEGIVATMYDGRTLHSREAIEILEENFGELLYSTRIRKTIRYAEAPVKGTSV
                     LKYDPTGPAAEAYRELAREVLQKEVLHGAEAREHA"
     gene            14688..16250
                     /gene="ftsZ"
                     /locus_tag="AC150V_07565"
     CDS             14688..16250
                     /gene="ftsZ"
                     /locus_tag="AC150V_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3762818.1"
                     /translation="MARKRASMREGPLAELFRATEAAKRGKGAEGEPSETPPERADET
                     PDENEQTQLITPEQDAREQAALEATVEHVYDFGADEETVVVPPAAASPPEPIEPPAST
                     PPEREPAGEPEVMAEPDPEPEPARVVELAPRRARRPERQPEAPPEAPYVPPASRFLQP
                     MPESAARLEVGPGTDTASYLAVIRVVGVGGAGLNAVNRMMDAGIAQVDFVAVNTDAQQ
                     LGLSDAPVKIHIGEELTHGLGSGSEPETGRLAAEESYDQLKQALRGSDMVFVTAGEGG
                     GTGTGAAPVVARIAREVGALTVGIVTTPFKFEGTRRRQASNHGVEALRAECDTLIVIP
                     NDRLLDVLERSTSMVDAFKVADDVLRQGVQGICDLITLPGLINLDFADVRTIMSDGGA
                     ALMGIGYSEAEEDRAREAADRALRSPLIDTEIVGARGILLSVAGGEDLTLLEVNEAAE
                     AVRRAATDDTNIIFGATVDERLNGQVWVTVVATGLGGTRRRTAVPALSGAGALAGRTP
                     ASGDTELPTFLR"
     gene            complement(16262..17233)
                     /locus_tag="AC150V_07570"
     CDS             complement(16262..17233)
                     /locus_tag="AC150V_07570"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR3762819.1"
                     /translation="MVFGVQTRYIVLATVSLVATAAALLTPQLLGGHFRVGVSGALAA
                     EPGGLWLAAVCFVGVMACSGIAWWTALRGCRSTASRMDAISRFAAGSLVNSLVPARAG
                     SAVRIALFARTLPGQGRILAAGGAGVAAGAARALWTAAIVAVAALTGNVPAWPAALAA
                     LFVAAAVTAAVVAPRVRLRRGLGHALDAFRALGTSPIQAARLIGWCGLAVAARLAAAA
                     AVAAAVGVESPFAIALLVVAAVDVAAALPVTPGNIGVAGAAVALVLRSAGVNGADALA
                     VGVAFSGLETLTAIVVGGAATAMLALRARLPVDGEPPAEPPTPRATG"
     gene            17315..18676
                     /locus_tag="AC150V_07575"
     CDS             17315..18676
                     /locus_tag="AC150V_07575"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR3762820.1"
                     /translation="MKGAVAAGHELTADAGARILAEGGNAVDGCVAAALAAFVVEGPL
                     TGAAGGGFLLHRSRSGAAVVYDCFFAVPSRSLGEMEATIIDFADSSTQVFHIGTGSVA
                     VPGLLKGLATVHAAHGKLPWRELVEPAVELARMGATLNDAQQFLHEILGAILLREEGG
                     RRIFGVKGRVVTEDLVPSLEAIRDEGASAVYEILPELAADIERYEVVERSPVRASFGG
                     AAVEATPAPSRGGEIVARGLECLGAGGLGGPVGSGEAAVGLARALAVGYGGESAGGLT
                     GTTHVSVVDADLEAAALSSTLGSGSGVFRAGCQLNNMLGELDVIGDEPRAPGDRLPSM
                     MTPTLISQEALPRLVLGSAGSVRLSGAILQVVASVVGYGLGVDEAIDRARLHPDGGRM
                     HLEGGVDEEAAAALAADGWELVRWSGRNLFFGGVGAVEIRPDGSLAAAGDPRRCGVGV
                     VVS"
BASE COUNT         2689 a         6525 c         6688 g         2866 t
ORIGIN      
        1 atgttcccgc cggggaggcc gctctcgggg tcgctcgact tccgcggggg ctccggcgtg
       61 acgacccagt cctcgacctc gccaaaggcc gccatgccct ccggccccgc gaggtcggga
      121 gagctccact tctgcttgac ctttcccgcg tcctccgcgt agtcgagacg gaaccggagc
      181 cagaggccct tcttcagggc ctcgcgtgac acgttgcggg ggaccgccac cgtgtactcg
      241 acgtcctcgt cggaggtgaa cgcttgcgtc ccgtggggct gcgtctccgt cttaccgacg
      301 tcggtgccga tgatcttgtc cttcttgtcg tcccacgtgc cgtcgtcctg ccagtcggcc
      361 caggcgttga ggtaggcagc gcgtttggtc ttgttctcgt tcttgttctt gttcttgttc
      421 ttcggttcgt agcggccgtc accggtgccg tccttccctt tgtggacgcg gatcttcacc
      481 ttcagctcta gcgtgccccc agggcgcagg tcggccttga cctcgacgcc gtcgtcggag
      541 tcgtcgtcga cctggcgggg atcgcactcg ccgctcacga cgccgtcgcc gagcttctcg
      601 aactcgtagg gcggcgcccc gagtaggtga atcgcccctt tgccagggtc gagaagcggt
      661 acgtcgttca gggtgcggtc gcactcggga ccgtgagtct gcgtctgccc gtcgtagcgc
      721 ctgtccgcgt caccgaggtc gggggtatag aggaagttga caccggcctt gtccgatgcg
      781 tgcagctccc gcctggctgt cgttggtacg gcgaagaaca tgatcgggtc gcgaagcgcg
      841 gcgtctgctt cagtcgcgtc ttcggaggtg tgtccgagcc cgacgaagtg cccgagctcg
      901 tgaatggcag tcgtctgcag atccacgacg accggtcgtt tggtgaggtc gtggtcggcc
      961 gcgtccagcg cccagcgtat gaagaggcgg tggagtgcga cgtcgctgtc gacgatctcc
     1021 tgttggacgc cgcgacgctt gaatcggcgc tcggggtctt gcggaaccgt cacgccaggc
     1081 tcgtcgggcc cgccccgtct ggcaaacagg aacacgttcg cctggtcgag cgcaacgggg
     1141 tcggcgttgc cgggccagtc ggctccagcg tcgtgtttga ggtcaattct gagcaccgac
     1201 ggcctgacag cgttccacac ggcgaccgct gcatcgatct gggcgcgcac gtccgcacgg
     1261 gagagcacgg cgccctcggg cccctttggc aggacgtcgt tcgcttcggc gtccttggtc
     1321 agcagtgaca gtggaaccct gcagttcttg agctggcgga gatcccagta gccctgctga
     1381 agcggcggca cctccgtcgg gagcggccac ttgttcgcac cgcgtttatt cagatcgaga
     1441 aagttcgtca ggcggaaaac gacgtcgccg tcctgtgccg cggcgcctag ggcgaagatg
     1501 gaggcggcca ggacgaggaa gacgactgct cggagccgga cgagcacgac actcgattat
     1561 gtgactttcg tctcaatctc gcaaggggcc gcacgcctgc agctagctct tgcgcaacgc
     1621 tctcgcgtcg acgacctcga acgccgagcc gggaacacga tggagcgagt ggaggtcgtc
     1681 gttgtcccag cgcgggtgcg gcgcgcccga gacgaaccag tcggagccgt tgtcggcgac
     1741 caccatcccg tactccttga gcgcccgcaa gatcacccgc gcctggggcg ggaagccgga
     1801 gatgtcgaag ttccgcttca gccgcaggcg cagccccatc ggcggtagtg acgcgtcgga
     1861 cgatgagctg gcgaaatggc gagcgggcca gacgtacgcc ctgcgcgtac gcgatacggt
     1921 gaagcggagc gcgtgcgcga tccgtccccg cgccacctct tcgtagcggg cgagaccggg
     1981 caggatcgga agtccggccg cgtctgcgga cgtccagccc ttcggccgca gacggttcga
     2041 ccggaggtcc catacggcac ccgacgcggc ccgccacccg gaaccggcag ggtagagcgc
     2101 gaacagctcg tagaggcggc agcgctcgcg gtcgacgatg atcgcgtgac ggtcgccttc
     2161 ggagcgcctg ccaccctcga tcttcacggc gcgcgggatc ggataggggc cacggtcgga
     2221 ctcggcgctg tactggaagc gaacacggct tcctttcacc cgccggccga ccacggtgat
     2281 cgggattccg atcgggccgc cctgccacag accggagccg aagtcggcgt ggagatgctc
     2341 gtccagtccg atcgaggcga cgatggcagt agaggtgggc gccgccggga atcgatccac
     2401 gcgctgattc cagaagttgt cggacgggaa gaccggacac cccgcgacgc gcggcccggc
     2461 ttcgggtgcg gcggacacga ccgacgcgac agcgagcgcg actgccgcga ggaacgcgat
     2521 ggtggccgcg gcgcgcatgg agcaggcatc gtcgcagccg gcgtggcgga acagccctct
     2581 gccgaggaac tcggccctac gagccctgcg cggagcgccg ctgcgccttg taggcgcgct
     2641 tcttcgcctg gtacgcctcg agcgcgtcca ctgagtcgac gtcgccgagc gtcggatgat
     2701 cgggaacgag tggctcggca gcctccacgc cgaagcgctg cgctagcacg gatccgagcg
     2761 ccttgatctt catctccccg aaaccgggga gagcgccgat ccgctgccgc aggtcgttcg
     2821 cgtcggcggc ctcggtccac acccgctcgg cgtgcccgcc gtactcacct tcgacgacgg
     2881 cgcagagctc tctgacgcgc cgtgccatgt tccccgggaa ccggtggatg gcgggcttct
     2941 cgcggaacac cgcctcgagc tcgtccgggt ctgtggccgc gatctcccca gccgcgagcg
     3001 ttccgagccg ccgctccagc ttcagcgggc cggcgaaggc ggtctgcacc ggaacctgct
     3061 gatcgagcgc aaacccgatc aggagcgcga gcggctctcg cgcgatgagc gcgtccgcct
     3121 cttcgtcacc ggtgaagtgg agagcatccg gtgtcttcat ctaccctccc cctgctcgtc
     3181 gaagccgtcc ggctcccgcg ccaggagctc ctcttggagt tcgccgtact cgtcgaccca
     3241 gacgcgtttc cggatgcggt cgcgggtcag aacggcctgg tcggcgcctc gcgactcgac
     3301 cgcccagccg tcgcgctgat acgcgttgag ggcggggccg aaccgcgcct gtcggtcttt
     3361 cgcagtcagg accggtgatg tgccgcccgc catccgcgcc tcgccacggc cacggcgccc
     3421 gagcgcgaac acaagcagga agagcagcac gatgcccgct ccgacgagcg cccaggccca
     3481 ccccggaacg cctgagtctc cgccgccgga gccacccgcg ccgttcgtgt cggtcgtatc
     3541 ggtggttgtc gtggtgtcgg tcgtttccgt cgtcgtgtca gtggtatccg ccgtgtcctg
     3601 cgcgaacgcg gcaggtgcgg tcagatgcgc cagcagcgcc gcgctcccga cgccgacggc
     3661 cgtgagagca aggacggcga cgagcagcac tgccctccgg cgggcggaca caaacaccgt
     3721 tcgatcctat ccagcggctc acggacggag aaaccgcacc ggtagattca ggccatggag
     3781 ccgctggcgg cgctcggcgc caatacgcga gagtggttcg cgcgcgcgtt cgccgccccg
     3841 acgccggctc aggaactggg ctggcctgcg atcgcgtctg gcgcacacgt gctgatccag
     3901 gcgccgaccg gctccggcaa gacgctcgcc gcattcctgg tcggcatcga ccgcctgaac
     3961 gacacgccgg gcgacggcct tcgtctcctt tacgtctccc cgctcaaggc gctcaactac
     4021 gacgtcgagc ggaacctgcg cggcccgctg gcggggctgc gatccgagct cagcgtcggt
     4081 gtccgcaccg gcgacacgcc gcagcgggag cgacagcaga tgctccgcag gcccccggac
     4141 atcctgatca cgactcccga gtcgcttttc ctcctgctca cgtctcaggc gcgccagacg
     4201 cttcgtggca tcgagaccgt cattctcgac gaggtgcatg cggtcgcggg cacgaaacgc
     4261 ggctctcacc tcgcgctctc gctcgaacgg ctggaggagc tcgccgaccg acccttccag
     4321 cggatcgggc tctcggcgac ccagcgtccg ctcgaagaga tcgggcgctt cgtctcgggc
     4381 ggccgcccga tcgagctggt cgacgccggg acacgcaagg agctcgacct cgaagtcgtc
     4441 gtccccgtcg aggacatgcg cgagctcggg tcgcgccagg cgctgtcgca gcccgtcgtc
     4501 cccgacggcg tcgcgatggg ctcgggctac gaaccgtcgg cgcgctcgat ctggccgtcg
     4561 atctacccgg ccgtcctcga cctcgtccgg cagcaccggt cgacgatcgt cttcgtcaac
     4621 aaccgccggc tggccgaacg gctggcaatc cggctcaacg agctcgccgg ggaagatctc
     4681 gcccgcgccc atcacggctc gctcgctcgc gagcagcgtg tcgagatcga ggagttactc
     4741 aaacgcggcg gcatcccctg cctcgtcgcc acctcgagcc tcgagctcgg catcgacatg
     4801 ggagccgtcg acctcgttgt ccaggtcgag agcccgaagt cggtcgcccg cgggctgcag
     4861 cgcgtcggcc gcgcggggca cgagctgggc tcggtctcga agggtcgcct gtttccgaag
     4921 ttccgagccg acctgctgga gtccgcggtc gtcgccaagg cgatgcgtga gggcgcgatc
     4981 gaagagacct cgatcccgcg gaacccgctc gacgtgctcg cgcagcagat cgtggcgatc
     5041 tgcgccgacg ccgagatcgg cgttgacgac ctgtaccggg tcgtccgcgg tgcctacccg
     5101 ttcgccgacc tctcgcggca gcagctggag aacgtcctcg acatgctggc cggacgctat
     5161 ccctccgacg agttcgccgc gctccgcccg cgcatcgtct gggatcgcac cggcggtgtg
     5221 atccgcggcc gcacgggagc ccgccgcctc gccgtgacga acgccggcac gatcccggac
     5281 cgagggctgt acggcgtctt tcagctcgac ggcggcggac gcgtcggtga gctcgacgag
     5341 gagatggtct acgaggctcg cgagggacag accttccttc tcggtgcctc gacctggcgc
     5401 atcgaggaga tcacccgcga ccgcgtgctg gtgtctcccg ctcccggcgt tccgggagcc
     5461 gtaccgttct ggaagggcga gggcgtcggt cgtccgttcg agctcggaga gcgaatcggg
     5521 cgggcatcgc gcgagctcgt gtcgctcacc gacgacaggg cgctcggccg actcggcgag
     5581 gactacgccc tcgagccgcg ggcagcccag aacctcctga cgtatctccg tgagcaggaa
     5641 gcggccacga gagccgtgcc ttcggaccgc acggtcgtcg tcgagcgctt ccgtgacgag
     5701 atcggcgact ggcgggtctg cattctgacc ccgttcggcg gccgcgtgca cgcaccgtgg
     5761 tcgatggccc tcgccgcgag gctccgcgag gcactcgggc tcgaagtgca gtcgctctgg
     5821 tccgatgacg gcatcgcgct ccatctcccg gaggccgact ctccgccccc gatcgacgac
     5881 ctcctcgttg ctcccgacga gatcgaggac ctcgtcgttc aggaggtggg gcagacggca
     5941 ctcttcggcg cccgcttccg cgagaatgcg gcccgttcgc tcctcatccc gaggcgccgg
     6001 cccgggcagc ggactccgct ctggcagcaa cgcctgaagg cgcagagcct gctccaggtc
     6061 gcccgtcgct acggctcgtt tcccgtcgtg ctcgagacgt accgggagtg cctccaggac
     6121 gtcttcgatc tgccggctct gaagcgcatc ctggagcggc tccaaacgcg cgagctcgat
     6181 ctggtcgacg tggagacgcc gtcggcgtcg cctttcgcgt cgtcgttgct gttcgactac
     6241 gtggccacgt acatgtacga ggacgacacg ccggccgctg agcggcgagc gcaggcgctt
     6301 tccctcgacc gtgagctgct gcgcgagctg ctcggggcgg aggagctccg ggagctcatc
     6361 gatcccgggg cgctggccga ggtcgagggg tcgctgcgtc tcagcccgag gaacccggac
     6421 gagctgcacg acctccttcg ccgggcgggt gagttgctgg agggggagtt cgacgcaggc
     6481 ttcgccgaga cgcttctgcg cgagcggcga gcgatccgcg ttccgatcgg cgggcggaac
     6541 ggactcgccg cggcggaaga cgcgggccgt taccgggacg cattcggcgc catgccgccc
     6601 ggagggctgc ccgacgcctt cctcgagccg gttccggacg cgctcgagtc cgtcgtacgg
     6661 cgcttcgcgc ggaccagtgg ccccttcacg actgccgagg ttgccgctcg atacgggctg
     6721 tccgaggaag aggccgacgc gttgctgggc gggctcgagc gagccgacct gctcgtgcgt
     6781 ggcgagctcc gcccgggcgg cgtggagcgc gaatggtgcg atcccgacgt cctgcgccgc
     6841 atacggcgcg cctcgctcgc ggttcttcgc cgggaggtcg agcccgcgga gcagacggcg
     6901 ctcggacgct ttctccccgc ttggcacggc atcggccggc gggcggagct tcgcgaggcg
     6961 ctcgtgccgc tccaggccct tcccctgccg gtctcgctct gggagaccga cgtgttgccg
     7021 cagcgcgtgc ccggctaccg accggagcag ctcgatgccc tgtgcgcgag cggtgagctg
     7081 gtctgggtgg gagccgggct cgaccgggtg gccgtctact tccgggacga cgcttccgtc
     7141 ctcggcccgc cggcaggcgc cgagtcgccg gctgcgccgg cgcacgacgc gctgcggctg
     7201 gtgctcacgc ggggcgccga gttctggccc gacctcgtcg cggcgagcgg gctcgagagt
     7261 gacgaggcgc tggcggctct ctgggagctg gtgtgggccg gcgaggtgac gaacgacgcc
     7321 tggtcgccgc ttcgcgctgc tcgccggtac ggaacccccg tcgcggagca gcggccgcgg
     7381 cgcttctcgc ggagccgggc ccgccgctcg acggcgacgc aggggcgctg gacgagggtc
     7441 gcacccgtct tcggtgagct tccggaccgg cgggcgttga gcgagttgct gctggagcgc
     7501 cagggaatcg tgacccgcga cggagtccgc ggcgagggca tccgcggcgg cttcggcgcc
     7561 gtctacggcg agctccgtgt gctggagacg gtgggggtct gtcgccgcgg ctacttcgtc
     7621 gaggggctcg gcggggcgca gttcgccctc gcgggcgccg tcgagcggct ccgggagctt
     7681 cgggccgagg acgacggtga cgcactcgtc ctcgcggcgg cggaccccgc tcagccctac
     7741 ggcgcggcct tgccgtggcc ccggcgggcc ggcgcgcgag cggcccgtgt ggccggcgct
     7801 cacgtcgtgc tgctgggcgg cgacgccgtg ctcttcgtcg agcgtggcgg tcggtcgctc
     7861 gtgccgctcc gcgatcctga cgagacgtgg ctccgcccgg cactggctgc gctggtcgcc
     7921 cgagtgaagg tcggaggcaa gcgtctggca gtcgagcgat tcgacggcgt gcccgtcacg
     7981 gattcggacg tgctcccgct tctcgtcgag gcgggctttc tcctcgggcc gcgtcgcgca
     8041 gtgctgcgcc cgtgacccgt accgtctggc tgctgggcga ccagctcgac cgcagtgggg
     8101 ccgctcttcg cgacgccgag gcgaattcga cgaccgtgct gctcgtcgag agcgcggcga
     8161 agctcgccga gaagcggtgg catcgacagc gtctccacct ggtgctggct gccatgcggc
     8221 gcttcgccga cgaactgcga gaggacggct tcgaagtcga ctaccgccac gcggcttcgc
     8281 tggagtccgg cgtgcgtgac cacctcgccg agagagagcc cgacgagctc gtcatcgagg
     8341 agcccatgtc ctgggacggc ctccgactcg cgaagcagct cggttgtcgc atcgtccgct
     8401 ccgagcgatt cctctgccac tacgacgagt tcgccgcgtg ggctcagggc cgcaagcgtc
     8461 tcgtcctcga gcacttttac cgcgaccagc gcgttcggca cggctacctg atggacggcg
     8521 acgagcccgc gggcggtcgg tggaacttcg acgccgacaa tcggcagccg cccccccgcg
     8581 acgaggtcga atggccgcgg ccgcctctcg aggccctcga cgctctcgac cacgaggtgc
     8641 tcgaagcgct cccggcggcg gtctggggcg aagcggccgt ggggctgtgg ccgacaaacc
     8701 ggaaccacgc gctcgcgctg ctgtcgcact tcgtcgacga ggtcctgccc ggcttcggcc
     8761 cgtacgagga cgccatgctc gccgaagagt ggcggttgcg acactcgctc ctctcgcccg
     8821 ccctgaacct gggactgctc catccacgcg aggtgtgcga cgcggtcgag cgtgcgtatc
     8881 gggccggggc cgtcccgatc gcctcggcgg aggggttcat ccggcaggtt ctcggctggc
     8941 gggagtatgt gtgggggatg tactgggcgg ccatgccgtc gtaccgcgac gagaacgagc
     9001 tagcggcgga ggaggagctt ccgccgctct accgcggcgg gccgacgcgg atgcactgcg
     9061 cctcggtgac gatccggggg gtcgaggagc gcgcctgggc ccaccacatc cagcgcctga
     9121 tggtgctcgg aaacctctcg ctgttggccg gcgtccgccc acgcgagctg gtcgagtgga
     9181 tgtgggcgtc cttcgtcgac ggcgcggagt gggtgatggt gccgaacgtg atcggcatgg
     9241 cgcttcacgc cgacggaggc cgcatggcga cgaagcccta cgcggcgggc ggtgcgtaca
     9301 tcaaccgcat gagcgactac tgccgagcgt gcgaattcga ccccaagcag cgcgtcggcg
     9361 aggacgcctg tccgttcacg accctctact gggacttcct cgaccggcag cgagaccggc
     9421 tggcggcgaa ccaccgtctc gggcggcagc tggcgaacct cgatcgcctt ggcgatctcg
     9481 acgcggttcg tgctcgggcg caggacgtgc gtcgccggct cgccgccggt gagctctgac
     9541 tcgcagcggt cagccggggc cggtacaggc cacgctacga acggattccc gtcgcgtacc
     9601 ggcgcaggcg ggcgacgttt gcctcgaagt caccgttcgc gtacgaggcg ccgagcgcct
     9661 tggccgcctt cgaggtgacg cccggaccgc ccagcgcgag cgcggccctc ggctcgtggg
     9721 ggaggtctgc cagtccgtcc ctgatcgcgt ccatcgcctc gtcgcgtgac gcgctgaggc
     9781 agagcagacc ggcgtcgagc aactccgcaa agccgacggc ctcctcgacc ggcgtgtcct
     9841 ggccgaggta ttcgacccgc cagccgtcgg cgcggaggag aaccgccagc atcagaagcc
     9901 cgaggtcgtg ttgctcgccc ggtgcgcacg ccagcactgc cgtgccacgg attcccccgc
     9961 gcagatctcc gaggaacgag tcgagccgag cccggacctg gcccgagagc agatgctcct
    10021 gggcgacgct gatacgtcca acttcccatt cgtccccgac ccagcggaga gcgggcgcga
    10081 tcaccttggc gatcgccggc tcgatcggga gcacggcgaa cgtctggtcg agcgtcgcgc
    10141 tgatctcgcc gctcgagctg tcgcgcgttg cctgtatcag cgcctcgagg aggcccgccg
    10201 gatccgctgc cgtcggccgg tgtgtctcct ccagcacgcg tgacgcctcc ccgatccgcc
    10261 agccctcgcg aagccggtca cgaagccatt ccacgcgtgc gacgtcctgc tcggtgtacc
    10321 gacgctggcc gccggtcgtg cgggtcggat ggagcacgcc gtagcgttgc tcccatttgc
    10381 gcagcgtgtc cggcgcgacc cccgtgcgcc gcgagagcgc cgagatgttg aggagattcg
    10441 ccatcgcaga cctggacgaa gcttagacca cctgtgtcac cattgcctca tgccggaggg
    10501 cgattcagtc catcgcgcag ctgcgaggct ccaggtgctg gtcggagagc gtctcgaggc
    10561 cgaggctccc catccacggg gggcggtgac cggcgtcgcc gcggccgtgg acgggaaggt
    10621 gctcgaggcg gtcgaggcca gaggcaagaa tctgctcctg cgcttcgagg gaggcgtgac
    10681 cgtacgcagt cacttgcgga tgagcgggcg ctggcgcgtc gaccggcgcg gcggccgccg
    10741 cacgggccgg ccgtggctcg tgctgcgcgg tcgtgagtgg gaggccgtcc agtggaacgg
    10801 gccggtactc acgctcgacg ggcgggaggc gatcaggcgg ctcgggccgg atctcctggt
    10861 ggcgacgacc gatccccacg acgtcgttcc gcgcctccgc cagtccgatc cgcgcaggct
    10921 gttgggagag gtgctggtcg accagcgact tgtcgccggg atcggaaaca tgtggctcgc
    10981 cgagtccctg tggcacgctc gcgcatcgcc ctggctttcg ctcggagagg cgagcgacgc
    11041 cgagctcgtt gccgcccttg cgcacgcgag caatgggatg cgcgacgctg cggccggccc
    11101 acgtccgctc cgcaaggtgt atcgacgggc cggcaggcca tgcccccgtt gcggcgcgac
    11161 gctccgctcc cgcggtctcg gtgatgcgaa tcgaaccgcc tactggtgcc cgcgctgcca
    11221 gcgcggcccg gatgtgtcgt gagccctctc gcagtctggc tggaaggaga gcgcagacct
    11281 tcggcgtagt gcggaaacgt gccgcttcgc gcaccgcatc tccatcttgc cctgcgaagc
    11341 ttcgcgctcg gggccttcgc gtatctcctc cgagagctcg acgacggcgg cgaagagctg
    11401 cccttcgcgt tcgaggagca tcctcagcgc gacgggcccg ctctttacga ataccgtccc
    11461 ctcgtccgcg acttcatcga gtcccgtgcc gatcggctac gaactcgcga cgacgcgatc
    11521 ctggcagtcg acgagctgcg ccgcgagccg acggcggcga tctttgcccg agcccacgcc
    11581 ggcgccactc catccgagga cgaggcgctc ttccggactg tgctcctggc gctgctcgtt
    11641 gccagcgcgg agagctgcgg cggcttcgac tgggacgacg cggctttcga gcacgcttac
    11701 gcagagctgg agcactcgct cttcggagaa caccgcgcct atcgcgcggt cgcgcccctg
    11761 acggggatct cggtcggcac tcgggtcgag ctctcagccg cgatccgcat gcgagcagcg
    11821 gccgacggcg agctcaaccg ccattggccc gaggcccgtg gcttgttgcc ggaggacttc
    11881 ggccgcgagg ccgaccgcta ctgcgtcctc gagctgcggg ccgatctcgg cccggcagac
    11941 gagccgcccg acgcgcccgc cgagatcgcg gacgcggtga gcgcggttcg tctcgcgacc
    12001 gccgcgccct tggcggcggg gccggtcttg ttcgagacac tcgacggtcg tcccttcggc
    12061 atccgcccgg tgctcccaat cgcggcgacc gagccgcccg gcgatcccgg ccggctcgat
    12121 gagtttcgcg gcacactggc cgtggagcta ctggcgcagc tcggcagcgc cgacggcgac
    12181 cctgcccttt ccgaggcgct cgatcgctgg gagctctcgc tctttcagca cgaccctttt
    12241 cgctccgagc agctccgcgg ctccttcacc gcgttgctcg gtgagacatg ggcgctgcgg
    12301 gcagcagtct tgctcgagga cgagccggac gggcggggtg ccctgcacca gcagctcgca
    12361 gctcttgcgg agggggccac ggcgaccccg gcagccacgg acgctgcgcg gcggatgctc
    12421 gtcgctgcgc ttcggcacgg cgaccgccac gagctcgtag gcgagctcga cggccggctg
    12481 ctcgggcttc gcgagcgggc cacgctcggg ctggcagtct gagcggaccg tcctgcactc
    12541 ggcacgatcg cgacgcgaag gcgtaacgac tcggccggta gcgtcggaag atggacgctg
    12601 cgcggagagt gctctccagg ctggcgcaga tcgaggcgct cgagcgggca gatgccccgc
    12661 cgatcgacct gctgagcgcc ctcgcgagcc ttgccgagga ggcggaagac tgggcggccg
    12721 cagagggagg cgaagaggcc cgagcggcag tcgcgcgatt acgcgctgcg ctgacccgtg
    12781 acatgatcgc aatgtgatca cgcactggga cgacgtcgac ggaatccggc gcaccgaagg
    12841 gcacatcgcc gccgactggc accctctcac cggggcgagc tcggtctccg tgggggtgcg
    12901 acgaatcaag gtcgatcccg gcatgtggtc cacgccgctc cacgtggagg ggtcggagga
    12961 ggagatcttc tacgtcctcg gcggaacggg aacgtccgtc cagtgggacg gcgagcacac
    13021 cgagtcgttc gagctcgctt ccggcgactg cctcgttcat ctcgctctcg agaacgcaca
    13081 tacgctccga gcgggagacg cagggatcga cgtccttgcg ttcgggcagc gccactacgc
    13141 accgggcgtg acggttctgc cgcgcgccga ggcggcctgg ctcggagcga gctggggtcg
    13201 cgtgggggga gccgacgacc acccgtggaa acgcgaggcg gcgcaggggc ccccggaggt
    13261 gtcgggtctc tcgccgcgcc ccgatcgaat cgtcaacgtc gaggacgtcc agccgatcga
    13321 gcacgtcggc gccaccgtgg gacgacggat ccgcgatctc gggcgagcgg ccggctcgga
    13381 acggaccggc ctacgtcact gcgaagtgct cccagggatg ctcaacgtgc cgccccactg
    13441 ccactcggcg gaggaagaga tcttcgtcgt gctcgccggc gagggagtgc tgctgctctg
    13501 gccgacgggg gccgacgcct ccgacgagca gcccgtcacg gcgggccata cgatctcgcg
    13561 gcccgccggc acgggggtcg cccacgcgtt ccgcggcggg gagaacggca tgagtctgct
    13621 gatgtacgga acccgtgagt cgggcgacgt ctgctactac ccgcggtcga acaagctctc
    13681 cttccgaggg ctgggagtga tcgcccgtct cgagctcctc gactactggg agggtgagga
    13741 ttgaaggaga agccgcggcg cccttgaacg agagtccgtc atgggactcc tgcgactgct
    13801 tccacgcccg gcgagccgac tcgacgaagt cgagcccgac gccgactcga ccgacgcgcc
    13861 cgacactccg gctagcgtgt caccacccga gatgatcttc gagccggacc tatcgagcga
    13921 ggggcggtcg caggtgcgcg tcctcgccct cgcgaaccag aagggcggcg tcgcgaagac
    13981 gacgactgcg ctcaatctcg gtgtcgcctt ccgcgagatg gggcaccgtg tgctgttgat
    14041 cgatctcgat ccgcagggca acctgaccat gagccagggc atgaacccgg acgtgatcga
    14101 gcggtcgatg ttcgacgtcc tcgtccaccg cattccgatc tccgatgtga tcgagcacac
    14161 cgaggtcgac ctcgcggtcg cctcgatcga tctcgccggc gccgagctgg ccctgagctc
    14221 gatgatcggt cgcgagcgct cgctcgagaa ggcgctcgtc gaggtacgcg accgatacga
    14281 cttcatcctg atcgacacgc cgccgtcgct cggcctgctg acgatcaacg cgttcgtcgg
    14341 ggcgacgggg gtcattgtcc cggtgcagtg cgaatatctc tcgcttcgag ggctcgtaca
    14401 gctcgagaac acgctggcga tggtccgtga gaacctgaac ccgcacgtgg gggtcgaggg
    14461 aatcgtcgcg acgatgtacg acggacggac actgcactcg cgcgaggcga tcgagatcct
    14521 cgaggagaac ttcggcgagc tgctctacag caccaggatt cgcaagacca ttcgctatgc
    14581 ggaagcgccg gtgaagggga cgtcggtcct caagtacgat ccgacgggac cagccgcgga
    14641 ggcctatcgc gagcttgcgc gggaggtgct ccagaaggag gtgctccatg gcgcggaagc
    14701 gcgcgagcat gcgtgagggg ccactcgccg agctgttccg cgcgacggag gctgccaagc
    14761 gcggtaaggg cgcggagggg gagccatcgg aaacaccacc cgagcgcgcc gacgagacgc
    14821 cggacgagaa cgagcagacc cagctgatca cgcccgagca ggatgcgcgc gagcaggcgg
    14881 cgctcgaggc gacggtcgag cacgtctacg acttcggtgc cgacgaggaa accgtcgtcg
    14941 tcccgccggc cgcggcttcc cctccggagc cgatcgagcc tccggcatcg acgccgccgg
    15001 agcgagagcc ggcaggcgag cccgaggtca tggccgagcc cgatccggag ccggagccgg
    15061 cccgcgtcgt cgagttggcg ccgcgccgcg cccgacggcc ggagcggcag ccggaggcgc
    15121 cgcccgaggc gccgtacgtg cctcccgcga gccgctttct ccagccgatg cccgagagcg
    15181 ccgcgcgcct cgaggtcggg ccgggaacag acaccgcctc ctatttggcg gtgatccgcg
    15241 tggtcggcgt cggcggtgca ggtctcaacg ccgtcaacag gatgatggat gccgggatcg
    15301 cacaggtcga cttcgtcgcg gtcaacaccg acgcccagca actcggcctg tcggacgctc
    15361 cggtcaagat tcatatcggc gaggagctca cgcacgggct cggttccggg tcggagcccg
    15421 agaccggccg tctcgcggcg gaggagtcgt acgaccagct caagcaggcg ctgcgcggct
    15481 ccgacatggt cttcgtcaca gcaggtgagg gcggcggcac cgggacgggg gccgcgccgg
    15541 tcgtcgcccg aatcgcccgc gaggtcggcg cgctcaccgt ggggatcgtc acgaccccct
    15601 tcaagttcga gggcacccgc cggcgacagg cctccaacca cggtgtcgaa gcgctcagag
    15661 ccgagtgcga cacgctgatc gtgattccga acgatcggct gctcgacgtg ctcgagcggt
    15721 cgacgtccat ggtggacgcc ttcaaggtcg cggacgacgt gctgcgccag ggcgtgcagg
    15781 gcatctgcga tctgatcacg ctgccggggt tgatcaacct cgacttcgcc gacgtccgga
    15841 cgatcatgtc cgacggtggc gcggccctga tgggcatcgg ctactcggag gccgaggaag
    15901 atcgcgctcg cgaggccgcc gaccgtgctc tccgttcacc gttgatcgac accgagatcg
    15961 tcggcgcgag gggcatcctc ctctcggtcg ccggtggcga ggatctgacg ctgctcgagg
    16021 tcaacgaggc ggccgaagcc gttcgtcgag cggccacgga cgacacgaac atcatctttg
    16081 gcgcgacggt cgacgagcgg ctgaacggcc aggtgtgggt gacagtcgtg gcgaccgggc
    16141 tcggcggcac gcggcgacga accgccgtcc ccgcactgtc aggagccgga gcgctcgcgg
    16201 gtcggacgcc cgcaagcgga gacacggagc tccccacctt cctccgctag agccctcgac
    16261 atcacccggt ggcccgcggc gtgggaggct ccgccggcgg ctctccatcg accggcaggc
    16321 gggcacgaag cgcaagcatg gcggtggccg cgccgccgac gacgatcgcc gtcagcgtct
    16381 cgaggccgct gaaggccaca ccgacggcga gcgcatcggc gccgttgacg cctgcggagc
    16441 ggagcacgag agcgacggcc gcgcctgcga cgccgatgtt gccgggcgtg accgggagcg
    16501 ccgcggccac gtcgacggcc gccacgacga ggagcgcgat agcgaaaggc gactcgacgc
    16561 cgaccgcggc cgcaaccgct gccgccgccg cgagccgcgc cgcgacggcc aggccgcacc
    16621 agccgatcag tcgcgccgcc tggatcggcg acgtgccgag agcacggaag gcgtccagcg
    16681 catgcccgag cccgcgtcgg aggcgaacgc gcggcgcgac gactgcggct gtcaccgccg
    16741 cggcgacgaa caaggcggcc agcgccgccg gccaggccgg gacattgccc gtcagggcgg
    16801 cgacagccac gatcgcggcc gtccagagcg cccgtgccgc tcctgccgca acgcccgcgc
    16861 cgccggctgc gaggatgcgc ccctgtccgg gcagcgttcg ggcgaacagc gcgatccgga
    16921 cggcgctccc ggcgcgggcg ggcacgagcg agttcaccag cgagcctgcc gcgaacctgg
    16981 agatcgcgtc catgcgtgag gccgtcgagc ggcaaccgcg aagcgccgtc caccaggcga
    17041 tcccgctgca tgccatcact cccacgaagc agaccgccgc cagccagagg cctcccggct
    17101 cggcggcgag ggcgccggac acgccgacgc ggaaatggcc gccgagcagt tgcggcgtga
    17161 gcagcgcggc cgccgtcgcg acgagcgaca cggtcgcgag cacgatgtat cgcgtttgga
    17221 ccccgaaaac catctggtgc atctctttcg acgtcgagaa cgcgttcgtg atggtggctc
    17281 cgggtcggca acacacgcgg ttacgctccc gctcatgaaa ggcgcagtcg cggcagggca
    17341 cgagctgacc gccgacgcgg gcgcccggat cctggccgag ggcggcaatg cggtcgacgg
    17401 atgcgtggcc gctgcgctgg cggcgttcgt cgtcgaaggt ccactcacgg gcgcggccgg
    17461 aggcggcttc ctcctccacc ggtcccgttc cggcgcagcg gtcgtctacg actgcttctt
    17521 cgccgtgccg tcgcgctcgc tcggggagat ggaggccacg atcatcgact tcgcggactc
    17581 ctcgacccag gtgttccaca tcggaacggg ctcggtcgcg gttccgggcc tgctgaaggg
    17641 tctggcgacg gtgcacgccg cacatggcaa gctcccgtgg cgcgagctcg tggagcccgc
    17701 agtcgagctt gcgcggatgg gcgccacgct caatgacgcc cagcagttcc tgcacgagat
    17761 cctcggcgcg attctgttgc gcgaggaagg cgggcggcgc atcttcggcg tgaagggcag
    17821 agtcgtgacc gaggatctcg ttccgtcgct cgaagcgatc cgcgacgagg gcgcgtcagc
    17881 cgtgtacgag attctccccg agctcgcggc cgacatcgag cgctacgagg tggtcgagcg
    17941 ctctccggtg cgcgcctcct tcgggggcgc cgcagtcgag gcgactccgg cgccgtcacg
    18001 cggtggagag atcgtggcgc gaggcctcga atgcctcggc gcgggcggcc tcggcggacc
    18061 ggtcggcagc ggtgaggcgg ccgtcgggct cgcccgggcg ctggcggtcg ggtacggcgg
    18121 ggaatccgcg ggcggactca ccggcacgac gcacgtctcg gtcgtcgatg cagacctcga
    18181 ggccgcagct ctctcgtcca cgctgggctc cggctccggc gtgtttcgcg ccgggtgcca
    18241 gctcaacaac atgctcggtg agctcgatgt catcggcgac gagcctcgcg cgcccggcga
    18301 tcgcctgccg agcatgatga cgccgacgct catcagtcag gaagcgctgc cgcgcctggt
    18361 gctcggcagc gccggatcag tgcggctctc gggtgcgatc cttcaggtcg tcgcctcggt
    18421 cgtcggatac gggctgggcg tggacgaggc gatcgaccgc gctcggcttc atcccgacgg
    18481 cggccgcatg cacctcgagg gcggtgtcga tgaggaggcg gccgccgcgc tcgcggcgga
    18541 cggctgggag ctcgttcgct ggagcggccg caacctcttc ttcggaggcg tcggggcggt
    18601 cgagatccgg ccggacggct cgctcgccgc ggcaggcgat ccgcgccggt gcggcgtcgg
    18661 cgtcgtcgtg agctgacggc tgggcgggaa aaactagcct tcccttgcgg ttcgcgcacg
    18721 gcttctatga gtcggtgcgc ccgagcgacg acgcggcccg gcgaatcg
//
LOCUS       DBNGNK010000257         3177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1691769,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000257 DBNGNK010000000
VERSION     DBNGNK010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1691769"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="AC150V_07580"
     CDS             complement(<1..1318)
                     /locus_tag="AC150V_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR3762821.1"
                     /translation="MPPLRTSDAYLPTGDQPRAIEQLVASVDAGNRYQTLLGATGTGK
                     TATMAWIIEELQRPALIIAHNKTLAAQLCNEFREFFPDNAVEYFVSYYDYYQPEAYVP
                     QADLYIEKDSSQNDDIARLRLGATSSLLTRRDVVVVASVSCIYGIGSPEEWRERVLIL
                     EVGSDHDRDEVLRTLIDSQYVRNDTVLARGRFRVKGDVVEVQPAASETAYRISFFGDE
                     VEQISHFDPLTGEVYSKLDNLAIWPATEYVTSTPTIDRALDEIRHELERQVAAFEKDD
                     RMLEAHRIRQRTEYDLEMMKELGFCNGIENYSRILEGRPPGSHPFTLIDYFPDDFVCF
                     VDESHQTVPQIGGMYEGDRSRKQTLVDFGFRLPSALDNRPLRFDEFERFSPQTIFVSA
                     TPGDYEQQHSGAVVEQVVRPTGLVDPVVEVRPASTQVDDLLSEIHKR"
     gene            1513..>3177
                     /locus_tag="AC150V_07585"
     CDS             1513..>3177
                     /locus_tag="AC150V_07585"
                     /inference="COORDINATES: protein motif:HMM:NF042648.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HIR3762822.1"
                     /translation="MRLTRRRLLAVGLGAATSLAAAPGAALAGKIRAPLLRGITVRNL
                     GRRYVGDRRLFATVSPGVPGQDTLTVAFALNRRAQVLLEAVRIQRRRPIVAWHAAQEL
                     EPGTHELPWTPAPDTPVGSYVLRLTLEDEQGARRIYGGSRPLDPARSRAPVARVLNVE
                     ASFDRRSYLPGEPMSLTLTADTPSLTLTFLRCGEEEVATGRNDEFSGVELGEPVRIDW
                     TGKRSTPTPIEVQSGNWPTGLYAVRLETPDGRLGFAPFILRPAVLGTRRQAAVLPTNT
                     WQAYNYYDADGDGWGDSWYAGGNPPVVLDRPYLHRGVPPRYRRYDLPFLRWLRRTGHN
                     PEMLAEDDLEAFPAGDDLRRLYDLVVFPGHTEYVTEHVYNVVERFRDLGGRLIFLSAN
                     NFFWRVDKDGNKLRRVALWRDLGRPEATLLGAQYRANDDGTRQAPFTVSGAAVTPWLF
                     EQTTLQNGSTFGEAIGGYGIEIDATTDGSPPGTHILARANGIFGTGLDAEMTYYETAS
                     GARVFNAGVLDFCSSVMTWPVWKLCDNLWRHMLTDVPAPPAPPPDQA"
BASE COUNT          482 a         1125 c         1057 g          513 t
ORIGIN      
        1 cccgcttgtg aatctccgac agtaggtcgt cgacctgggt actcgccggg cgcacctcga
       61 ccaccggatc gaccaggccg gtcggccgca ccacctgctc gaccacggcg ccgctgtgct
      121 gctgctcgta gtcgccgggc gtggccgaga cgaagatcgt ctgcggcgag aagcgctcga
      181 attcgtcgaa acgcagcggg cggttgtcga gcgccgacgg caggcggaag ccgaaatcga
      241 cgagcgtctg cttacgcgac ctgtccccct cgtacatgcc accgatctgt ggcaccgtct
      301 gatgcgactc gtcgacgaag cagacgaagt cgtcgggaaa gtagtcgatc agcgtgaacg
      361 ggtgagaccc gggcggccgc ccttcgagga tgcgcgagta gttctcgatc ccgttgcaga
      421 agcccagctc cttcatcatc tcgaggtcgt attccgtccg ctgccggatc cgatgggctt
      481 cgagcatccg atcgtccttc tcgaacgccg ccacctgccg ctccagctcg tgccggatct
      541 cgtcgagcgc gcggtcgatg gtcggcgtcg acgtcacgta ctccgttgcc ggccagatcg
      601 cgaggttgtc gagcttcgag tagacctcgc ctgtgagcgg gtcgaagtgg gagatctgct
      661 cgacctcgtc gccgaagaac gagatgcggt acgccgtctc ggaggcggcc ggctgcacct
      721 cgacgacgtc gcccttgacg cggaagcggc ctcgcgcgag cacggtgtcg ttgcggacgt
      781 actggctgtc gatcagcgtg cgcagcacct cgtcgcggtc gtggtccgag ccgacctcga
      841 ggatcaggac gcgctctcgc cactcctcgg gcgagccgat cccgtagatg cacgacaccg
      901 acgcgaccac gacgacgtcg cggcgcgtga gcaatgacga ggtggcccca aggcgcagcc
      961 tggcgatgtc gtcgttctgc gacgagtcct tctcgatgta gaggtcggcc tgcggcacgt
     1021 acgcctcggg ctggtagtag tcgtagtagg agacgaagta ctcgacggcg ttgtcgggaa
     1081 agaactcccg gaactcgttg cagagctggg cggcgagcgt cttgttgtgg gcgatgatga
     1141 gcgcgggtcg ttgcagctcc tcgatgatcc acgccatcgt cgccgtcttt cccgttccgg
     1201 tcgcgccgag cagtgtctgg tagcggttgc ccgcatccac cgatgcgacc agctgctcga
     1261 tcgcacgagg ctggtcgccg gtcggcaagt aggcgtcgga ggtgcgcagc ggcggcaccg
     1321 tgccagcgta cccggcggcc ggtcgcgtac gacctgatct tctgggctcc ccggctcacc
     1381 gtgacgggcg gtcgcctgcg agccgtgctc ggagagacgg actacgtcct tccgggtgtt
     1441 cagcgacgcc ttccgcagag ggccgaacgg tcctaacggc tccgtaacac gaacgccgta
     1501 gagtgcaccc ggatgcgact cacgcgccgc cgcctgctgg ccgtcggatt gggcgccgca
     1561 acatcgctcg ccgcggcccc cggggcggcg ctcgcgggca agattcgcgc gccgctgctc
     1621 aggggcataa ccgttcgcaa cctcggacgg cgatacgtcg gcgaccggcg gctgttcgcg
     1681 acggtcagcc cgggcgtacc cggccaggac acgctcaccg tcgccttcgc gctcaaccgg
     1741 cgagcgcagg ttctcctgga ggcagtgcgg atccagcgtc gccgaccgat cgtggcctgg
     1801 cacgcggcgc aggagctcga gccgggcacg cacgaactgc cgtggacgcc cgcccccgac
     1861 actccggtcg ggtcgtacgt gctccgcctc accctggagg acgagcaggg cgcccgccgc
     1921 atctacggcg ggtcgcgccc tctggatccc gcgcggtcgc gcgcaccggt ggcccgcgtg
     1981 ctcaacgtcg aagcctcatt cgacaggcgc tcgtaccttc cgggcgagcc tatgagcctc
     2041 accctcacgg ccgatacccc gtcactcacg ctgaccttcc tccgctgcgg cgaggaagag
     2101 gtcgccacgg ggcgaaacga cgagttcagc ggtgtcgagc tcggcgagcc tgtgcgcatc
     2161 gactggaccg gcaagcgctc cacgccgacg ccgatcgagg ttcagagcgg taactggcct
     2221 accggcctgt acgcggtccg cctcgagacg cccgacggcc ggctcgggtt tgcccctttc
     2281 atcctccgcc cggcggtgct cggcacgcgg cgacaggccg cggtcctccc caccaacacg
     2341 tggcaggcgt acaactacta cgacgccgac ggcgacggct ggggcgattc ctggtatgcg
     2401 ggcggcaacc cgcctgtcgt cctcgaccgt ccgtacctcc accgcggcgt gcctcctcgc
     2461 tatcgacgct acgacctgcc gttcctccgt tggctccgcc ggacaggcca caaccccgag
     2521 atgctcgcgg aggatgatct cgaggctttc cctgcggggg acgatctgcg gcggctctac
     2581 gatctcgtcg tcttcccggg ccacaccgag tacgtgaccg agcacgtgta caacgtcgtg
     2641 gagcgcttcc gcgacctggg cggacgcctg atcttcctct ccgccaacaa cttcttctgg
     2701 cgcgtggaca aggacgggaa caagctccgc cgggtcgcgc tctggcgcga tctcgggcga
     2761 cccgaggcga ccctgctcgg cgcccagtac cgcgccaacg acgacgggac gcggcaggcg
     2821 ccgttcaccg tgagcggcgc cgcggtcaca ccctggctgt tcgagcagac gacgctgcag
     2881 aacgggtcga cgttcggcga ggcgatcggc ggctacggga tcgagatcga cgccacgacg
     2941 gacggctcgc cgcccggcac gcacatcctc gcgcgcgcca acgggatctt cggcaccggc
     3001 ctcgacgcgg agatgacgta ctacgagacc gcgtcggggg cgcgcgtctt caacgccggc
     3061 gtcctcgact tctgctcctc ggtgatgacc tggccggtct ggaagctctg cgacaacctc
     3121 tggcgccaca tgctcaccga cgtccccgca cctcccgctc ccccgccgga tcaggcg
//
LOCUS       DBNGNK010000258         3455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1981035,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000258 DBNGNK010000000
VERSION     DBNGNK010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1981035"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1077)
                     /gene="pstC"
                     /locus_tag="AC150V_07590"
     CDS             complement(136..1077)
                     /gene="pstC"
                     /locus_tag="AC150V_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR3762823.1"
                     /translation="MTSQDVAIQPAAGAPQLSSKRVRWGEAVIRGILALAALISVLTT
                     LGIVMALLLPAVDFFRAVNPIDFLTGTDWAPLFEPGSFGVLPLVVGTIMISLWSALVA
                     FPLGLGTAIYLSEYARPKVRATLKPILEILAAIPTVVLGYFALTWVTPFLRDTLGVDA
                     EIFNALSASLVLGIMLIPTVASLSEDAMASVPRDLRDGGYGLGADKLQVSTRIVVPAA
                     ISGIIAAFVLGFSRAVGETMIVLIAAGQLPNLTFDPRETVETMTAFIGATGNGDIPTG
                     SIEYKTIFAVGLTLFVITLILNVISIRLVRRYREEYE"
     gene            complement(1079..2038)
                     /locus_tag="AC150V_07595"
     CDS             complement(1079..2038)
                     /locus_tag="AC150V_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR3762824.1"
                     /translation="MGRVVAESEEGLRLTTLLGALFLLAVVVIVASECSGGEGGSGGT
                     IEADGSSTVGPYVTRAAEVFRRENADVRITIGISGTGGGFDRFCRGETDLSNASREIK
                     DEEAALCEDNGVEFTKFDVANDALTVIRNPGNDWAVCLTTDQLNAIWEPGSKVDNWSK
                     VSGEFPAERMPLYGPGTDSGTFDYFTDVINGEEGASRTDFSATEDDNVILQGVTGEKG
                     GLGYLGFSYFEENQDRVAAVEVDGGSGCVAPSVETTQDGSYAPLSRPLFVYVRNDGLA
                     RGEVQDFMRFMLDNQAEIAESAQFVPLNDEQTAEFEQQLEERN"
     gene            complement(2101..3036)
                     /locus_tag="AC150V_07600"
     CDS             complement(2101..3036)
                     /locus_tag="AC150V_07600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3762825.1"
                     /translation="MDGDAKRGRIVVEESREAVVVLDDQDRVMLASRRARESIDGLRE
                     GEPLPSGYLSGERGVVPLIIPYELHGQRERVVYLSQAGDLTAYEELRAGFTAAVSHEL
                     RTPLARLLSLLETAALPGENVAELVEQARAEVEQIRELIDEVLFISELESGSRVVSLG
                     SVRVVPEFEAVAAELAFRADRAGVSLVVEGDPELELEIRPRMLRVIAINLADNAVRYA
                     GPGATMTLSVWREANGVVVRGADDGAGVDEDELPRLFERFFRSDRSRASRGTGLGLAI
                     VKHIVTQAGGRVEASGGSEGGLEIRCTFPPGVAGA"
     gene            3153..>3455
                     /locus_tag="AC150V_07605"
     CDS             3153..>3455
                     /locus_tag="AC150V_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3762826.1"
                     /translation="MKRTTLLLAALAALALAVAATATAAVIKGTTGDDTLDGTPRADL
                     IRAFAGNDTIDAGSGPDVIRAGKGDDRANGGDGRDLVFGGKGNDTLAGGAAKDRIWG"
BASE COUNT          610 a         1189 c         1143 g          513 t
ORIGIN      
        1 cggtcagcag cttccaatcg agctccggca gagcggtgag gaacgtatcg acgagcagaa
       61 tcccgagcac gccgagcgag atcccgagcg agccgatcat caggcccttg aagagattgt
      121 tcttcagctt gcgactcatt cgtattcctc tcgatagcga cgcaccagcc ggatcgagat
      181 cacgttgagg atgagcgtga tgacgaacag cgtcaggccg acggcgaaga tcgtcttgta
      241 ctcgatcgat ccggtcggga tgtcgccgtt gccggtggca ccgatgaacg ccgtcatcgt
      301 ctcgaccgtc tcgcgcgggt cgaacgtaag gttcgggagc tgcccggcgg cgatcagcac
      361 gatcatcgtc tcgcccaccg cgcgcgagaa cccgaggacg aacgcggcga tgatccccga
      421 gatcgcggcg ggaacgacga tcctggtcga gacctgcagc ttgtcggcgc ccagaccgta
      481 gccgccgtcg cggagatcac gcggaacgga cgccatcgcg tcctccgaga gcgaggccac
      541 cgtcgggatg agcatgatcc cgagcacgag gctggccgac agcgcgttga agatctcggc
      601 gtcgacgccg agcgtgtcgc ggaggaacgg cgtcacccac gtgagcgcga agtacccgag
      661 caccaccgtc gggatcgccg cgaggatctc gagaatcggc ttcagcgtgg cgcgcacctt
      721 cggccgcgcg tactcgctga ggtagatcgc tgttcccaac ccgagcggga aggcgacgag
      781 cgccgaccag agcgagatca tgatcgtgcc gacgacgaga ggcaggacgc cgaaactgcc
      841 cggctcgaac agcggtgccc agtcggttcc cgtcaggaag tcgatcgggt tgacggcgcg
      901 gaagaagtcg accgccggca gcagcagcgc catcacgatc ccgagtgtcg tcagcaccga
      961 gatcagcgcc gccagcgcga ggatgcccct gatgacggcc tccccccagc gaacgcgctt
     1021 cgacgacagc tgcggtgcgc cggcggccgg ctgaatcgcg acgtcctggc tggtcacgct
     1081 agttccgttc ctccagctgc tgctcgaact ccgcggtctg ctcgtcgttg agcggaacga
     1141 actgggccga ctcggcgatc tccgcctggt tgtcgagcat gaaccgcatg aagtcctgga
     1201 cctcgccgcg cgccaacccg tcgttgcgca cgtagacgaa aagagggcga gagagcgggg
     1261 cgtaggagcc gtcctgcgtg gtctccacgc tcggggcgac gcagccggag ccgccgtcga
     1321 cctcgaccgc ggcgacgcga tcctggttct cctcgaagta ggagaagccg aggtagccga
     1381 ggccgccctt ctcgcccgta acgccctgca gaatgacgtt gtcgtcctca gtggcggaga
     1441 aatccgtgcg gctggccccc tcctcgccat tgattacatc ggtgaagtag tcgaacgtgc
     1501 cggagtcggt gcccggcccg tagagaggca ttcgctcagc ggggaactcg cccgagacct
     1561 tgctccaatt gtcgaccttc gagcccggct cccagatcgc gttgagctga tcggttgtga
     1621 ggcagaccgc ccagtcgttg ccggggtttc ggatcaccgt gagggcgtcg ttggcgacgt
     1681 cgaacttcgt gaactcgacg ccgttgtctt cgcagagcgc ggcctcctcg tccttgatct
     1741 cacgggacgc gttcgagagg tcggtctcgc cgcggcagaa gcgatcgaag ccgccgcccg
     1801 ttccggagat cccgatggtg atcctcacgt cggcgttctc gcgccggaac acttcggccg
     1861 cgcgcgtgac gtacgggccg acggtactcg agccgtcggc ctcgatcgtg ccgccgctgc
     1921 caccttcgcc gcctgagcat tcggaagcga cgatcacgac cactgcaagc aggaagagcg
     1981 cgccgagcag cgtcgtcagg cgcaaaccct cctcactctc ggcgacgaca cgtcccaagt
     2041 ctcgcgccgc ctcgaagctg tgtgggagtc aggcttgtga agaactggta acgaactggt
     2101 ttacgcgccc gccacgcccg gaggaaaggt gcagcggatc tcgagtccgc cttccgagcc
     2161 gccgctcgct tcgacgcgcc cgccggcctg cgtgacgatg tgcttgacga tcgccaggcc
     2221 gagccccgtc ccgcgcgagg cccgcgagcg atccgagcgg aagaatcgct cgaacagccg
     2281 tggcagctcg tcctcgtcga cgccggcccc gtcatcggct ccccgtacca ccacaccgtt
     2341 cgcctcacgc caaaccgaga gcgtcatggt tgcgcccggc cctgcgtagc ggacggcgtt
     2401 gtcggcgaga ttgatcgcga tcacccgcag catgcgcggc cggatctcga gctcgagctc
     2461 gggatcgccc tctacgacca gcgagacgcc ggcgcggtcg gcccggaagg ccagctcggc
     2521 ggcgaccgcc tcgaactcgg ggacgactcg cacagagccg agcgagacta cgcgcgagcc
     2581 ggactcgagc tccgagatga agagcacctc gtcgatcagc tcgcggatct gctcgacctc
     2641 ggcgcgggcc tgctcgacga gctcggcgac gttctcaccc gggagcgcgg ccgtctcgag
     2701 cagcgagagg aggcgcgcga gcggagtgcg cagctcgtgg gacacggccg cggtgaagcc
     2761 ggcacgaagc tcctcgtagg cggtgagatc gccggcctgg ctcagataca cgacacgctc
     2821 gcgctgccca tgcagttcgt acgggatgat caagggcacg acgcctcgct cgccagacag
     2881 gtagccgctc ggcagcggct ctccctcacg cagaccgtcg atcgattccc gcgcgcgccg
     2941 gctggcgagc atgacgcggt cctggtcgtc gagcacgacg accgcctcac ggctctcctc
     3001 gacgacgatc cggccccttt tcgcgtcgcc atccatgcac acagcgttct aacagaaggc
     3061 ttcatgaacc tttgccaccg ccgcatcgcc agctcacacg ccgcgacgac agtcactcca
     3121 tggacacgca ccatcgccaa ggaggcaaga cgatgaagcg cacgaccctt ctgctagcgg
     3181 cgctcgcagc gctggccctg gccgtcgcgg cgacggcgac cgcggcggtg atcaagggaa
     3241 ctacgggcga cgacaccctc gacggcactc cgcgcgccga cctgatccgg gcgttcgcgg
     3301 gcaacgacac gatcgacgcc ggctcgggcc ccgacgtcat acgcgccggc aagggcgacg
     3361 accgggcgaa cggcggcgac ggtcgcgacc tcgtgttcgg cggcaagggc aacgacacgc
     3421 tcgccggcgg ggccgcgaaa gaccggatct ggggc
//
LOCUS       DBNGNK010000259        11925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1334923,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000259 DBNGNK010000000
VERSION     DBNGNK010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1334923"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="AC150V_07610"
     CDS             <1..402
                     /locus_tag="AC150V_07610"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3762827.1"
                     /translation="KVDVDANQELAGRYSVQSIPAVKAFRDGRVVSEFTGARPAVSVN
                     SFIDELLAPPRADSLLEELRAAGELPAVVAALDDGDTERALELIVAAVPGASPEERDR
                     LRDVAVALFERLGQDDPLAVAYRRRLASALY"
     gene            complement(369..1403)
                     /locus_tag="AC150V_07615"
     CDS             complement(369..1403)
                     /locus_tag="AC150V_07615"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 71/99 protein"
                     /protein_id="HIR3762828.1"
                     /translation="MPGGRVTCVSLLGAIALALALPVGVQARGSDPPGPPAAEVAVFY
                     YPWYGNPRNDGRYMHWGQNRNTPPVRIASAFYPTGGVYSSASGAVLRRHMREIAAAGA
                     TTIVVSWWGPQSAEDARLPAVVANARAHGLRVALHVEPYASRSPLVLVSEIQRLLPLG
                     ISDVYVYQPSLSPDYEWRDANERLGGVRVFGHTHLPGRATAGGFDGLYTYDVLTFDGS
                     SFGRMCEGARRLGLLCAPSVGPGFDAHRATGDTRVRPRDAGATYDAMWSAAIGARPDI
                     VTVTSFNEWHEGTQIEPARCEDRYSCYEGAWSLRGPAAETAYLQRTAMWSHRYAEHMA
                     QYRAEASRRR"
     gene            1570..2607
                     /locus_tag="AC150V_07620"
     CDS             1570..2607
                     /locus_tag="AC150V_07620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3762829.1"
                     /translation="MRLLLTGYHGYVGSVVGPMLVDAGHEVVGLDAHLYEGCDFTPGA
                     PLSRAIRLDIRDVTKEHLTAFEGVVHLAALSNDPLGDIDPGLTHQINSVATARLAEIA
                     REAGVSRFVFASSCSVYGAAAPDGLVDEHAPLAPITPYAESKVAAEQRLLALADERFS
                     PISLRFATAYGASPRLRLDVVLNNLAGWAHTTGRVRLLSDGRAWRPLIHVEDMGAAVV
                     SVLAAPPAVVRGEAFNAGAVGANYLVRDLAEVVAATAPGSEVEFAEGSGSDRRSYRVD
                     FSKLADALPDYKPRWDPEKGAVQLVEAYRQAGLTREVFESDRFVRLARLRSLQSEGIL
                     DDSLRRRNGSA"
     gene            2594..3841
                     /locus_tag="AC150V_07625"
     CDS             2594..3841
                     /locus_tag="AC150V_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015162403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3762830.1"
                     /translation="MARPEPSAPPCRFCGAPLTFTVVDLGMSPLCENFLAPEQADAME
                     PFFPLHVRACEDCLLVQLSEYVAPDEIFREYAYFSSFSDSWLAHARRYTDDVAERFGL
                     NGNSFVVELASNDGYLIRNFVARGVRCLGIEPALNVAEAARAEGVETLTEFFGVGTAR
                     SVTSSYGSADLIVANNVFAHVPDINDFAGGVAALLAPTGVATFEFPHLVRLVEGLQYD
                     TIYHEHFSYLTLLTSGRILEAHGLTIFDVQELSTHGGSLRIYACHAGSRRVEPAVEQL
                     AGRERDAGYGKLDVYGGFRARVEESKRALLEFLIGARRDGKHVAAYGAPGKGNTLLNY
                     CGIRTDLVDYAVDRNPYKHGRLTPGTHIPIHPPEKLQETKPDLILILPWNLREEIAAQ
                     LAYTRDWGAELIVPIPLVEKVQG"
     gene            3875..4708
                     /locus_tag="AC150V_07630"
     CDS             3875..4708
                     /locus_tag="AC150V_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3762831.1"
                     /translation="MKVVLFCGGLGLRMREASERIPKPMVPIGNRPVLWHVMKYFAHY
                     GHKDFILCLGHKAEVIKEFFLNYEEAFSNDFVLDGSGNVELLSSDAHDWRITFVNTGL
                     QAKVGERLAAVREHLDGEEYFLANYGDVLTDAPLPHMIDTLRESGKVGSVLTVRPNYT
                     FHVISMDDGPHVTEIQDVTTAGIWINGGYFVFRHDVFEYLHPGEDLVVEPFARMISRE
                     QLLGYRYEGFWAPMDTLKDRNVLESLLEAGEAPWRVWEPRRENGTHDAVERAFARKLA
                     E"
     gene            4705..5361
                     /locus_tag="AC150V_07635"
     CDS             4705..5361
                     /locus_tag="AC150V_07635"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3762832.1"
                     /translation="MIELVLARPPDRPLQVLALGAHSDDIEIGCGGTILQLLADDTDV
                     RVDWVVLSARGERGEEAARSADGFLAGATGQRVVLGDFRDGFLPYSGPSVKEFFEQLK
                     AEVEPDVILTHNRSDLHQDHRLVCELTWNTWRNHLVLEYEVAKYDGDLGSPNVFVPLT
                     EETLERKMSLLLEHFPTQAGKHWFTRDAFASLPRLRGIESASPSGLAEAYYGRKLRLT
                     "
     gene            5358..5822
                     /locus_tag="AC150V_07640"
     CDS             5358..5822
                     /locus_tag="AC150V_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR3762833.1"
                     /translation="MSDAPRIEGVEVIPLRRIPDERGTIMHMLRRDDPHFREFGEIYF
                     TTIYRGVVKGWHRHTAMTLNYACVSGRVKLVLYDDREDSPTRETLMEVFLGPDDYSLV
                     VIPPGVWNGMKGMSEPHALVANCATHPHDSTRTSRVDPFDNHIPYDWGVRHS"
     gene            complement(5809..6741)
                     /locus_tag="AC150V_07645"
     CDS             complement(5809..6741)
                     /locus_tag="AC150V_07645"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3762834.1"
                     /translation="MSDRSAKSPAASLVGEPVLVTGGGGFIGSKLVTKLARAGVVVHA
                     SGRRPRSGDAAVWEVCDVADHGAVKEVVRRVRPRVVWHLAATAVGQSDPEIVVPMFRT
                     NALGTPNVLDACREVGNVRVVVAGSMEEPRGDEPPRTPYQASKAAASVLADLYRETYS
                     LDARRLRIHMVYGPGEREHTMLIPHVIESLLAGSVPRLRDGRRRVDWVYVDDVVDALL
                     AAAAVDVEAPGPVDVGTGVLTSVADVVTEVVGHFDGATFEVGAEADAADDRARAADVA
                     ATFERLGWRASTSLSEGIAQTVSWWRARADATSA"
     gene            complement(6738..7997)
                     /locus_tag="AC150V_07650"
     CDS             complement(6738..7997)
                     /locus_tag="AC150V_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HIR3762835.1"
                     /translation="MHEFARELMPLHRSITGAGLRHTLACISRRVPLELTEIASGTAV
                     LDWVIPREWSIREAWIKGPDGSKVVDLAWSTLHVVSYSVPVHERLSLEALRPHLHTIP
                     EQPSLIPYRTTYYEPGWGFCLAQETLDAMEDGEYEVLIDSSLEPGSLTLGEAVIGSGK
                     SEILFSMHICHPALANDNLSAVVTGVALAEILAAADLRCRYRFIFAPATIGAIAWLAT
                     HLGLLTDVRAGLVLAGVGDPGQITYKHSRSGDSLIDRTVTAALADLDIPYGVRPFTPL
                     GYDERQYGSPGFALAVGSIMRTPPGEYPEYHTSADDLDFVTPGALAGSLAALLRIVVL
                     LEENETLRNTAPYGEPQLGRRGLYDPLGGVPTSRELQRAVLWVLSLSDGNNDLLGIAE
                     RSGISFTTIREAADLLSEKSLLVPVDP"
     gene            complement(8084..9016)
                     /locus_tag="AC150V_07655"
     CDS             complement(8084..9016)
                     /locus_tag="AC150V_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762836.1"
                     /translation="MSTSLSFVGTVGLLNSLRLTGHDHEVIVLDAGLEPRQRALLDSR
                     CRVVDVPGASETNPLLFKPFPALLTLGGVVVVVDSDVIVTASLEPLIARAQAGAICAL
                     PDSDPTRFFPEWESVFALRTPLRRATYVNTALVFVHAERWRALLERWWQLSSTIEPAA
                     TSAFGADYGTPRADGDQDAFNALAMSEVDAGALEVLPAEVGPLSPLVHPRLGEVEVVD
                     EDSLACRFGETPTIMLHHAGRKPWISPLVDQNAYTCLLRRLLFHGDVELRLEPSDVPF
                     WLRPGRAAATAAQGLRLAAPLIRGVGGRIRSAVR"
     gene            complement(9138..9212)
                     /locus_tag="AC150V_07660"
     tRNA            complement(9138..9212)
                     /locus_tag="AC150V_07660"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9175..9177),aa:Pro,seq:ggg)
     gene            complement(9246..9542)
                     /locus_tag="AC150V_07665"
     CDS             complement(9246..9542)
                     /locus_tag="AC150V_07665"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3762837.1"
                     /translation="MARRRKYSDDSLGPTIESLMTETGITYRGLAERSGLSAGYLNHI
                     VHGNRPVPSNDVLARLAEALGVEPEYFREYRIRVITEKLESMPDLVDRLYKRLG"
     gene            complement(9542..11224)
                     /locus_tag="AC150V_07670"
     CDS             complement(9542..11224)
                     /locus_tag="AC150V_07670"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR3762838.1"
                     /translation="MLQRQSSLFLPTLRDDPAEAEAVSHKLLVRGGYIRQLGAGVWTY
                     LPAGWRIHQKVVQIIREEIDAIGGQELLMPVLTPAELWQESGRYDIPEVFKLQDRHGR
                     PFVLPMTHEETVTFHAREIQSYKQLPKILYHFSVKERDEPRPRGGLIRVREFVMKDSY
                     SFDRDADGLDVSFRAHAGAYERIFERCGLEVHAVQAESGMMGGSESIDYLAPAGSGEN
                     TLVTCANCDYAADLEIARGIPRPADFPEPLGAPQEVATPGVTTIDALAELLAVDPAAT
                     SKAMPVTKPDGAIVLALVRGDDRLSESKLIGVLGADFRPATEEEIKTAFGADPGSIGP
                     VGADLEIIADDALRTGEFVAGANRTGWHVRGVEAGRDFVPRFADIREPLEGDSCPRCS
                     GRLHFQTAIEVGHIFKLGTRYSQPLRCTYLDEGGTERPIVMGSYGVGPGRVLAAVVEQ
                     SHDDSGILWPPSIAPYDVHVLALAGGADEVSAMAEATAAALDEAGFAVLLDDRDARPG
                     EKFADADLLGAPFRVTVGRKSLEDDAVDVRERSSDADERVAVGSVLNWIEER"
     gene            complement(11237..>11925)
                     /locus_tag="AC150V_07675"
     CDS             complement(11237..>11925)
                     /locus_tag="AC150V_07675"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR3762839.1"
                     /translation="VAAAIEEVELLERLDYRDFKISVKSTHVPTMIRAYRMLAAKMPY
                     PLHLGVTEAGTPFAGAIKSSVGIGSLLADGIGDTFRVSLTADPVKEVEVAWEILKALG
                     LRERGPVMIACPSCGRDNVGVESLARVVEERLREYPQAFEVAVMGCAVNGPGEAGDAD
                     FGIAGGRDVGFVYAHGRVLKKVPSEILIDELFVEIDRWIREGMDRPKRLKMAKPAALA
                     MAEASLIPLD"
BASE COUNT         1959 a         4113 c         3936 g         1917 t
ORIGIN      
        1 aaggtcgacg tcgacgctaa ccaggagctc gccggtcgct actcggtgca gagcattcca
       61 gcggtcaagg cgttccgcga cgggcgcgtc gtctccgagt tcaccggcgc ccgccccgca
      121 gtctccgtga acagcttcat cgacgagctg ctggcgccac ccagagcgga cagcctgctc
      181 gaggagctcc gcgcagccgg cgagcttccg gccgttgtcg ctgcgctcga cgacggtgac
      241 acagagcggg cgctcgagct gatcgtggcg gccgtgccgg gcgcatcccc ggaagagcgc
      301 gaccgcttgc gagacgtggc cgttgccctc ttcgagcgat tgggtcaaga cgatccgctg
      361 gcagtggcct accgccgccg gctcgcttcg gctctgtact gagccatgtg ctctgcgtat
      421 cggtgcgacc acatcgccgt gcgctggaga taggctgttt cggctgccgg tccccgcagg
      481 ctccaggcgc cctcgtagca cgagtacctg tcctcacagc gcgccggctc gatctgggtg
      541 ccctcgtgcc actcgttgaa gctcgtcacc gtcacgatgt ccgggcgggc accgatggca
      601 gcgctccaca tcgcatcgta ggtggcaccc gcgtcgcgtg ggcgcacgcg tgtgtccccg
      661 gtcgcgcgat gcgcgtcgaa gcccggcccc accgacggag cgcagagcag gcccagcctt
      721 cgggccccct cgcacatccg cccgaaagag gagccgtcga aggtcaggac gtcgtacgtg
      781 taaaggccgt cgaacccgcc ggccgtcgcg cgcccgggca agtgcgtgtg accgaacaca
      841 cgaactccgc ccagtcgttc gttggcatcg cgccactcgt agtcggggga gagcgacggc
      901 tggtagacgt agacgtcgga gatgccgaga gggagtaggc gctggatctc cgacacgagc
      961 acgagcggag atcgactcgc gtacggctcg acgtgcaacg caacgcgaag tccgtgtgcg
     1021 cgcgcgttgg cgacgaccgc cggcagccgc gcgtcctcgg ccgactgtgg gccccaccag
     1081 gagaccacga tcgtcgtcgc gccggcggcc gcgatctcgc gcatatgccg gcgaagcacg
     1141 gctccactcg ccgacgagta gacacctccg gtcgggtaga acgccgatgc gatccggacg
     1201 ggcggcgtgt tccggttctg gccccagtgc atgtaccgcc cgtcgttgcg ggggtttccg
     1261 taccacgggt agtagaagac ggccacctcg gccgccggtg ggcccggcgg gtcgctcccg
     1321 cgagcctgga cgcccaccgg gagcgccagc gcgagcgcga tggctccgag cagcgagacg
     1381 cacgtgaccc tccctccagg caaaccgcgc ggactatacg cgggtgaagg gcccgcgtga
     1441 aggccggtct cgtcgagtgc ggcgcggagc cgggaccccc gacggacatc cctcgaacga
     1501 gggattggcg caaggaacga tccgaagccg cccgcaaccg gggatgcgtc tctacgatga
     1561 gcttcgggca tgcggcttct cctaaccggg taccacggct acgtcggctc cgtcgtcgga
     1621 ccgatgctcg tcgacgctgg acacgaggtg gtgggcctcg acgcccacct gtacgagggc
     1681 tgcgacttca cacccggcgc gccgctctcg agggcgatcc ggctcgacat tcgcgatgtc
     1741 acgaaggagc atttgacggc cttcgaaggg gtcgtccacc tcgcggcgct ctcgaacgac
     1801 ccgctcggcg acatcgaccc cggactgacg caccagatca acagcgtcgc gaccgcccgc
     1861 ctggcggaga tcgcgcgcga ggccggcgtc agccgattcg tgttcgcctc gtcctgcagc
     1921 gtctacggcg ccgccgctcc ggacgggctc gtcgacgagc acgctccgct tgccccgatc
     1981 accccctacg ccgagtccaa ggtcgccgcg gagcaaagac tgctggccct ggcggacgag
     2041 cgcttctccc ccatctcgct ccgctttgcg accgcctacg gggcgtcccc gcgcctccgg
     2101 ctcgacgtcg ttctcaacaa cctcgccggc tgggcgcaca cgaccggacg cgtccggctg
     2161 ctcagcgacg gccgggcgtg gcgcccgctc atccatgtcg aggacatggg agccgcggtg
     2221 gtttcggtgc tcgcagcgcc tccggccgtc gtccgtggag aggcgttcaa cgccggtgcc
     2281 gtcggagcca actacctggt tcgagatctc gcggaggtcg tcgcagcgac ggctccgggc
     2341 tccgaggtcg agttcgccga gggcagcggc tctgaccggc gcagctaccg cgtcgacttc
     2401 agcaagctcg cagacgcgct tccggactac aagccccgct gggatcccga gaagggcgcc
     2461 gtccagctcg tcgaggccta tcgacaggct ggactgaccc gcgaggtctt cgagagcgac
     2521 cgcttcgttc ggctcgcccg actccgctcg ctccaatccg agggcatcct cgacgactcc
     2581 ctgcggagga gaaatggctc ggcctgaacc ctctgccccg ccttgccgct tctgcggggc
     2641 accgttgacg ttcacggtcg tcgacctcgg gatgtcgcct ctctgcgaga acttcctggc
     2701 gccggagcag gcggacgcca tggagccttt cttcccgctc cacgttcggg cctgcgagga
     2761 ctgcctgctc gtccagctct cggagtacgt cgcgccggac gagatctttc gcgagtacgc
     2821 ctacttctcg tccttctccg attcctggct ggctcacgca cgccggtaca ccgatgacgt
     2881 cgccgagcgg ttcggcctca acgggaacag cttcgtggtc gagcttgcct ccaacgacgg
     2941 ctacctgatc cggaacttcg ttgctcgagg agtccgctgc ctcgggatcg agcccgcact
     3001 caacgtggcc gaggcagctc gggcggaggg cgtcgagaca ctgaccgagt tcttcggggt
     3061 cgggacggcg cgctcggtga cttcgtcgta cggcagcgcc gacttgatcg tggccaacaa
     3121 cgtcttcgcg cacgttcccg acatcaacga cttcgccggc ggcgtcgctg ccctcctcgc
     3181 tccgaccggc gtggcgacat tcgagttccc gcatctcgtg cggctcgtcg agggacttca
     3241 gtacgacacg atctaccacg agcacttctc ctacctcacg ctcctgacgt ccggccggat
     3301 cctcgaagcg cacgggctga cgatcttcga cgtccaggag ctctcgacgc acggcggctc
     3361 gctgcgaatc tacgcgtgcc acgccggctc gcggcgcgtc gagccggcgg tcgagcagct
     3421 cgcgggccgc gagcgggacg ccggctacgg caagctcgac gtctacggcg gcttccgcgc
     3481 gcgcgtcgag gagagcaagc gtgcgctgct cgagttcctg atcggtgctc gccgcgacgg
     3541 aaaacacgtc gccgcctacg gtgcgccggg gaagggcaac acccttctca actactgcgg
     3601 gattcgcacc gacctggtgg actacgccgt cgaccggaat ccgtacaagc acggccgact
     3661 gacccccggc acgcacatcc cgatccaccc gcccgagaag ctgcaggaga cgaagccgga
     3721 tctgatcctg atccttccct ggaatctccg tgaagagatc gcggcacagc tcgcgtatac
     3781 gcgcgactgg ggagcggagc tgatcgtccc catcccgctg gtggagaagg ttcagggttg
     3841 acggctgcgc ctcgcgagtc aggatgaggg tgggatgaaa gtcgtactgt tttgcggcgg
     3901 gctggggctt cgcatgcgcg aagcgtcgga gcggatcccg aagccgatgg tcccgatcgg
     3961 caatcgcccc gtcctgtggc acgtgatgaa gtacttcgcc cactacgggc acaaagactt
     4021 catcctctgc ctcgggcaca aggcagaggt gatcaaggag ttcttcctca actacgaaga
     4081 ggctttctcg aacgacttcg tcctcgacgg cagcggcaac gtggagctgc tctcgagcga
     4141 cgcgcacgat tggcggatca cgttcgtcaa caccggactc caggccaagg tcggcgagcg
     4201 gctcgcagcc gttcgagagc atctcgacgg cgaggagtac ttcctcgcca actacggtga
     4261 cgtcctgacg gacgctccct tgccgcacat gatcgacacg ttgagggaga gcggcaaggt
     4321 cgggagcgtt ctcacggtgc ggccgaacta cacctttcac gtgatctcga tggacgacgg
     4381 gccacatgtc accgagatcc aggacgtcac gacggctggg atctggatca acggcgggta
     4441 cttcgtcttc cggcacgacg tcttcgagta cctccacccc ggcgaggacc tcgtcgtcga
     4501 gccgttcgcg cgcatgatct cgcgcgaaca gctgctcggc tatcgctacg agggcttctg
     4561 ggcgccgatg gacacgctca aggacaggaa cgtcctcgag tcgctcctcg aagccggcga
     4621 ggctccctgg cgtgtctggg agccgcgacg cgagaacgga acacacgacg cggtcgagcg
     4681 agcgttcgcg cgcaagctgg ccgaatgatc gagctcgtgc tcgcgcggcc gcccgaccgg
     4741 ccgctgcaag tcctcgcgct cggcgcgcac tcggacgaca tcgagatcgg ctgcggcggg
     4801 acgatcctgc agctcttagc cgacgacacc gacgtgcgcg tcgactgggt cgtgctgagc
     4861 gcccggggcg agcgcggcga ggaagccgcg cggagcgcag acgggttcct tgccggcgcc
     4921 acagggcaac gggtcgttct cggagacttc cgtgacggct tcctccccta ctccggcccg
     4981 tcggtgaagg agtttttcga acagctcaag gccgaggtcg agcccgacgt gatcctcacc
     5041 cacaaccgga gcgatctgca tcaggatcac cggctcgtct gcgagctcac ctggaacacc
     5101 tggcgcaacc atctcgtgct cgagtacgaa gtggcgaaat acgacggtga tctcggctcc
     5161 cccaacgtgt tcgtgccgct gaccgaagag acgctcgagc ggaagatgtc gctcttgctg
     5221 gagcacttcc cgacgcaggc cggcaaacac tggttcacgc gcgacgcgtt cgcgtcgttg
     5281 cctcggctgc gcggcatcga gtcagcatct ccgagcggcc tcgcagaggc ctactacggc
     5341 cgcaagctga ggctcacgtg agcgatgcac ctcggatcga gggagtcgag gtcatcccgc
     5401 tacgtcgcat tccggacgag cgcgggacga tcatgcacat gctgcggcgc gacgatccgc
     5461 acttccggga gttcggcgag atctacttca cgacgatcta ccggggcgtc gtcaaggggt
     5521 ggcacaggca caccgccatg acgctcaact acgcgtgcgt cagcggccgc gtcaagctcg
     5581 tcctgtacga cgatcgtgag gactcaccca cgcgtgagac gctgatggag gtctttctcg
     5641 gtccggacga ctattcgctg gtcgtgatcc cgcccggggt gtggaacggg atgaagggga
     5701 tgtcggagcc gcatgctctc gtggcgaact gcgcgacgca cccgcacgat tcgacgcgaa
     5761 cgtctcgggt cgatccgttc gacaaccaca tcccctacga ctggggcgtc aggcactcgt
     5821 agcgtccgct cgcgcccgcc accacgatac ggtctgcgcg attccctccg acagcgaagt
     5881 cgaagcccgc cagccgagcc gttcgaaggt cgccgcgaca tcggcagcgc gagcccggtc
     5941 gtcggcagcg tctgcctccg cacccacctc gaacgtcgct ccgtcgaaat ggccgacgac
     6001 ctccgtcacg acatcggcga ccgacgtcag gacgcccgta ccgacgtcga cgggcccggg
     6061 agcttccacg tcgaccgcgg ctgcggcgag gagcgcgtcg acgacgtcgt ccacgtacac
     6121 ccagtccact cggcgccgcc cgtcgcgcag ccgtggcacg gagcccgcga ggagcgattc
     6181 gatcacatgc gggatcagca tcgtgtgctc tcgttctccc ggcccgtaca ccatgtgaat
     6241 ccggagccgc cgggcgtcca ggctataggt ctcgcgatag agatccgcga gcacggacgc
     6301 cgccgccttg gatgcctggt acggcgttcg cggcggctcg tctccgcggg gctcttccat
     6361 cgagccggcc accacgacgc ggacgttccc gacctcgcgg cacgcatcga gcacgttggg
     6421 cgttccgagc gcgttggtcc ggaacatcgg cacgacgatc tccggatccg attgccccac
     6481 ggccgtcgct gcgaggtgcc agacgacgcg cggtcgtacg cgccgcacga cctctttgac
     6541 ggcgccgtgg tcggcgacat cgcagacctc ccacacggcg gcatcgccgc ttcgcggtcg
     6601 tcggcccgac gcatggacca ccaccccggc gcgcgcgagc ttcgtcacga gctttgagcc
     6661 gatgaatccc cctcccccgg tgacgagcac gggctctccc accaacgacg ccgcgggcga
     6721 tttcgcgctc cgatcgctca cgggtctacg ggaacgagaa gcgacttctc ggacaagagg
     6781 tcggccgcct cgcgaatggt cgtgaaggag atccccgacc gctcggcgat gccgagaagg
     6841 tcgttgttgc cgtccgacag gctgagcacc cagaggacgg cgcgttggag ctcccgcgac
     6901 gtggggactc cgcccagcgg gtcgtacagt ccccttcggc cgagttgggg ctcgccgtaa
     6961 ggcgcggtgt tgcggagggt ttcgttttcc tccaggagca cgacgattcg gagcagcgcg
     7021 gccagggacc ctgccagcgc tcccggcgtg acgaagtcga gatcgtctgc cgacgtgtgg
     7081 tactcgggat actcaccggg aggagttcgc atgatagagc cgaccgccag cgcaaatccg
     7141 ggcgagccgt actggcgctc gtcgtagccg agcggtgtga agggccggac gccgtagggg
     7201 atgtcgagat cggcgagcgc ggccgtcacg gtccggtcga tgagcgagtc tcccgagcga
     7261 gagtgcttgt aggtgatctg gcccggatcg ccgacgccgg cgagcacgag gccagcgcgg
     7321 acatccgtga gaagtccgag gtgtgtcgcg agccaagcga tcgccccgat cgtggccggc
     7381 gcgaagatga accggtatct gcaccgtaga tcggcagcgg cgaggatttc ggccagcgcg
     7441 acaccggtga cgactgccga caggttgtcg ttcgcaaggg ccgggtggca gatgtgcatc
     7501 gaaaagagga tctcgctctt tcccgagccg atcacagcct cgccgagcgt gagcgagccg
     7561 ggctcgagcg agctgtcgat cagcacctcg tactccccgt cctccattgc gtcgagcgtt
     7621 tcctgagcaa ggcagaagcc ccaccccggt tcgtagtagg tcgtccgata cgggatcagc
     7681 gacggttgtt cgggaatcgt gtggaggtgc gggcgcaacg cctcgagaga aagcctctcg
     7741 tgcaccggca cgctgtagct cacgacatga agagtcgacc acgcgagatc tacgaccttc
     7801 gacccgtccg ggcccttgat ccaggcttcc cggatgctcc actcccgggg aatcacccag
     7861 tccaggaccg ctgtcccact cgcgatctca gtgagctcga gcggcacgcg gcgggaaata
     7921 catgccagcg tatgccggag cccagcgccc gtgatgctgc ggtggagcgg catcagctcg
     7981 cgtgcgaact cgtgcatgcg cgcgccgagc acctcgagat ccacgtcgtt ggcgagctcc
     8041 cgcagcggag ggcggggatt cggtgcgtca ggctcgggaa cgttcatctg accgcgctcc
     8101 tgatgcgacc gccgaccccg cggatgaggg gtgctgcgag tcggaggccc tgagccgctg
     8161 tcgcggcggc ccgtcccgga cgaagccaaa aagggacgtc gctcggctcg aggcgaagct
     8221 cgacgtcgcc gtggaacaac agccgccgta ggaggcaggt gtatgcgttc tggtccacaa
     8281 gaggagagat ccacggcttg cggcctgcgt ggtgaagcat gatcgtcggc gtctcgccga
     8341 accgacaggc aagagagtcc tcgtcgacga cctcgacctc acccagccgg ggatggacaa
     8401 gcggcgacag tggaccgacc tcggccggca gcacctccaa ggctcccgcg tcgacctcgc
     8461 tcatcgcaag cgcgttgaac gcatcctggt cgccgtcggc gcgtggcgtg ccgtagtcag
     8521 ccccgaacgc gctcgttgcg gccggctcga tggtggaaga cagctgccac catcgctcga
     8581 ggagagctct ccagcgctcc gcgtgaacga acacgagggc ggtgttgacg tatgtggccc
     8641 ttcggagtgg agtcctcagc gcgaagaccg attcccactc cgggaagaag cgggtcggat
     8701 ccgagtcggg aagcgcacag atcgcgcccg cctgcgcacg cgcgatgagt ggctcgagcg
     8761 atgccgtcac gatcacgtcg ctgtcgacca cgacgacgac gccaccgagc gtgagcagcg
     8821 cggggaacgg cttgaacagc agagggttcg tctcggacgc gccgggcacg tcgaccaccc
     8881 ggcagcgcga gtcgagcaga gcacgctgcc gtggctccaa cccggcgtcg aggacgatga
     8941 cctcgtggtc gtggccggta agccggagcg agttgagcag cccgactgtc ccgacgaagg
     9001 agagactcgt cgacacggca cagaaagccc cgcgcgaaga tgtgctgccc ggattcacgt
     9061 ctggcgtccc tgcccgatcg gcttccacag gcaaagcgtc gttgcagcgg ggctctccgt
     9121 caacgccacg ctgtgagtcg gggcggccag attcgaactg gcgaccttcg gtcccccaga
     9181 ccgacgcgct aaccaggctg cgccacgccc cgtgggtggc ctaccttagc ggacgtcgcc
     9241 tggcttcagc cgaggcgctt gtagaggcgg tcgacgaggt ccggcatcga ctcgagcttc
     9301 tccgtgatca cgcggatgcg gtactcgcgg aagtactcgg gctcgacgcc cagcgcctcc
     9361 gccagccgtg ccagcacgtc gttcgaggga acgggtcgat tgccgtgcac gatgtggttc
     9421 aggtacccgg ccgagagccc gcttcgttcg gctaggcccc ggtaggtgat cccggtctcc
     9481 gtcatcaggc tctcgatcgt ggggccgagc gaatcgtcac tgtatttccg gcgccgtgcc
     9541 atcacctctc ctcgatccag ttcaagactg atccgacggc gactcgctcg tctgcgtcgg
     9601 atgatctctc tctcacgtcg accgcgtcgt cctcgagcga ctttcggccg acggtcactc
     9661 ggaacggggc accgagtaga tccgcgtccg cgaacttctc accgggacgg gcatcgcggt
     9721 cgtccagcag aaccgcgaag cctgcctcgt ccagcgcggc cgcggtcgcc tcggccatcg
     9781 cagagacctc gtcggctccg ccggcgagcg cgagcacgtg cacgtcgtac ggcgcaatcg
     9841 agggcggcca gaggatcccg ctgtcgtcgt ggctctgctc gaccacggct gcgagtacgc
     9901 ggccgggccc gaccccgtag ctccccatca cgatgggccg ctccgtgccg ccctcgtcga
     9961 ggtacgtgca gcgcaggggc tgcgagtagc gagttccgag cttgaagatg tgtcccacct
    10021 cgattgcggt ctgaaagtga aggcgcccgc tgcaccgtgg gcacgagtcg ccctccagcg
    10081 gctcccggat gtcggcgaag cgaggtacga agtcgcgccc tgcctcaacg ccgcgaacgt
    10141 gccagccggt tcgattcgcg ccggccacga actcgcccgt ccggagcgca tcgtcggcga
    10201 tgatctcgag gtctgctccc acagggccga tcgagccggg atcggcaccg aaggccgtct
    10261 tgatctcctc ttcggtcgcc gggcggaagt ccgcacccag cacgccgatc agcttcgact
    10321 ccgacagacg gtcgtcgccg cgcacgagcg caagcacgat cgccccgtcc ggcttggtca
    10381 ccggcatcgc cttcgacgtg gcggcggggt cgacggcgag cagctctgcg agtgcatcta
    10441 tcgtggtcac gccgggtgtc gccacctctt ggggggcgcc gagaggctcc gggaagtcgg
    10501 ccggccgtgg aataccgcgc gcgatctcaa gatccgctgc gtaatcgcag ttcgcgcacg
    10561 tgaccagcgt gttctccccg gaccccgccg gggcgaggta gtcgatcgac tcgctgcccc
    10621 ccatcatccc cgattccgct tgaaccgcat gcacctcgag tccgcaccgc tcgaagatcc
    10681 gctcataggc gccggcgtgg gcgcggaagc tgacgtcgag tccgtcggcg tcgcgatcga
    10741 acgagtagga gtccttcatg acgaactcgc gcacccggat gagtcccccg cgcgggcgtg
    10801 gctcatcgcg ctccttcacc gagaagtgat agaggatctt cggcagctgc ttgtagctct
    10861 ggatctcccg cgcatggaac gtgaccgtct cctcgtgcgt catcggtagc acgaacggcc
    10921 gcccgtggcg atcctgcagc ttgaacacct ccggaatgtc gtagcgcccg ctctcctgcc
    10981 acagctctgc cggggtgagg acgggcatca gcagctcctg gccgccgatt gcgtcgatct
    11041 cctcgcggat gatctggacg accttctggt ggatgcgcca gcccgctggc aggtacgtcc
    11101 acacccccgc gcccagctgc cggatgtagc cgccgcgcac gagcagcttg tgcgagacgg
    11161 cttcggcttc ggccgggtcg tcgcgcaggg tcggaagaaa gagggaagac tggcgttgga
    11221 gcactgaccg cctaggctag tcgagtggga tcagggacgc ctcggccatc gcgagcgccg
    11281 ccggcttggc catcttcagg cgcttcggtc ggtccatccc ctcccggatc cagcgatcga
    11341 tctcgacgaa cagctcgtcg atcagtatct ccgacggaac cttcttcagc acgcgaccgt
    11401 gcgcgtagac gaagccgacg tcccggccgc cggcgatacc gaagtccgca tcccccgcct
    11461 cgccgggacc gttcaccgcg caccccatca cggcgacctc gaacgcttgc gggtactcgc
    11521 ggagccgctc ctcgacgacg cgtgcgaggc tctcgacccc tacgttgtcg cgtccgcaag
    11581 aaggacaggc gatcatcacc ggaccccgct cgcggagccc gagcgccttg aggatctccc
    11641 aggcgacctc gacctccttc accgggtcgg cggtcagcga gacgcgaaag gtgtccccga
    11701 tcccgtccgc cagcagcgag ccgatcccga ccgagctctt gatcgctcct gcgaacggag
    11761 tgcccgcctc ggtgaccccg agatgaagcg ggtacggcat cttggcggcc agcatgcggt
    11821 aggccctgat catcgtgggc acgtgagtcg acttgaccga gatcttgaag tcacggtaat
    11881 cgagccgctc gagcagctcg acctcttcga tcgccgcggc aacga
//
LOCUS       DBNGNK010000260         4970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_476456,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000260 DBNGNK010000000
VERSION     DBNGNK010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_476456"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="AC150V_07680"
     CDS             complement(<1..349)
                     /locus_tag="AC150V_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR3762840.1"
                     /translation="MLLYTVFALPFTVWMMYTYFRQMPPSREEAALVDGCTRIQALRK
                     IAWPLAKPGVVSAAAFAFIFSWTEFLFAVVLARDHAVTLPVALAGMAASQQGNLFGEA
                     SALTRVSLIPALLL"
     gene            complement(392..844)
                     /locus_tag="AC150V_07685"
     CDS             complement(392..844)
                     /locus_tag="AC150V_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease family protein"
                     /protein_id="HIR3762841.1"
                     /translation="MSEAAQPRRESEDSAKIILGWRLRVGWLRYLVVGAYLLFLLVPL
                     YWVFVASIKPQEDLLATPPIWWPDTLTDLHYAEALNRFNGWKGLKNSLIIASITTVAA
                     TVVGTAAAYSIARYRTGGKHLSFWFLSQRVLPPIAVIIPIFLLYSRYS"
     gene            complement(846..2735)
                     /locus_tag="AC150V_07690"
     CDS             complement(846..2735)
                     /locus_tag="AC150V_07690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3762842.1"
                     /translation="MHSTQLTQLSENLFCLADTCNVYVIRDGDAALLIDAGSGAVEGR
                     LAEIGVESVQWILHTHHHRDQCWGTERLAQSGARVAVPEYERHLFDRVEEFWQTRRVF
                     DNYDDRNTFFTLGQSVAVDAVLEDYDEFEWRGHRFLVLPAKGHTFGSSSLIAEIDGQK
                     VAFTGDLMSAGGTLYQLHAMEYMYSGLEGLVFTLQSIQALRRQEVSLALPSHGDPITD
                     VDADISRLENGIMDCVRLGRGMRIGGIWNVPEADYLPKLELYPLSPHLLWGGESTCSN
                     FYVLLSDSGKALFFDYGTANLAHLHIHADHGGLETLRFVEHRLDTLRDDHGVKEIDVV
                     VPTHIHDDHTCGIPYLQKFHGTECWALDIVAEVIENPSAWASTPCVYPKPIRVDRRLA
                     NGERFTWEEYELEIHHAPGQTEFHSVIATVVDGRTVAFTGDNYMEDEVVRGGSAVVLP
                     YQTTVLRNSFQLDMHHRCAEVMRSIGPDLVCPGHGPVLECSDAALDDYCAFIDEKDRV
                     FRGLVSAPADHYVDLFWARLLPYVATVAPGDEVVYRLLLRNNLERPATYSARLLAPDG
                     WVSADGTGELALDAGGRGELALRARAPGSGDSRRRLMTAEVAIDGRSQGPISEALVTV
                     VAAGS"
     gene            2846..4144
                     /locus_tag="AC150V_07695"
     CDS             2846..4144
                     /locus_tag="AC150V_07695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3762843.1"
                     /translation="MTPDVVIVGGGAIGATTAWELAQRGMAVTLLERGPTLAAGCSAG
                     NAGLICPSHSAPLPNPAALRNGLRWMLTPDSPLYLRPRPGVAPWLVRFVAACRPARAE
                     AGTRIIRELSIASLELHARIAEQGLETGFERRGTLNAYESDEGLRQARIEAEHLGLPT
                     RALDRKEIHALEPALSESVIGGVHYVDEAHCEPIGYVQAVADAAADAGAEIRTGVEVT
                     ALRRRDGRVAVVTAEGEIAPETVILAAGAWTSQLARQVGVFLPLEGGKGYHIDLEPVP
                     GDPRVPVFVQEARTIATPLSDRLRLSGTLELAGLDLTISPRRVDAIRRAIGRVLRFQE
                     DRKVMEVWAGLRPCTPDGLPVIGRVPGLDGLVLVTGHAMKGLSLAPITGRLVSQLVGG
                     EEPDFDLAPLRPGRFRPLLRRAGLRRTGAPRSAAQNARPT"
     gene            4147..>4970
                     /locus_tag="AC150V_07700"
     CDS             4147..>4970
                     /locus_tag="AC150V_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3762844.1"
                     /translation="MKSAGMRICSLLPSATEIVGELGMADRLVGRSAECDWPPEVREL
                     PVVTASRVDTATVASSTIDDAVRASIADGRSLYALDHELINQLAPDFVITQDLCAVCA
                     VSSADVGELCAVDAEVVSLDPPTLAGIEQSVAELARRLDAPESGAEVVARMRQTIGRV
                     RELVEGRPRPRGFVAEWLDPPFAAGHWVPEMVASAGGEEILGRAGKASFATSWEAVRD
                     GSPELVVLAPCGFDAERARDEADALPPLACRTVAVDANSYFSRPAPRVADGVAQLG"
BASE COUNT          771 a         1725 c         1727 g          747 t
ORIGIN      
        1 cgagtagcag cgccgggatc agcgacaccc tcgtcagagc gctcgcctcg ccgaagaggt
       61 tgccctgctg cgaggcggcc atgcccgcca gagcgacggg cagggtgaca gcgtggtcgc
      121 gggcgaggac gaccgcgaac aagaactcgg tccaggagaa gatgaacgcg aacgccgccg
      181 ccgagacgac acccggcttc gccaacggcc aggcgatctt gcgcaaggcc tggatccgcg
      241 tgcacccgtc caccagtgcc gcctcctccc gcgagggcgg catctgccgg aagtacgtgt
      301 acatcatcca gacggtgaac ggcagcgcga acaccgtgta caggagcacg aggccggttc
      361 gcgtgtcgat cagggcgtag ccgaaccagt cctacgagta gcggctgtag aggaggaaga
      421 tcgggatgat cacggcgatc ggcggcagca cccgctgcga caggaaccag aaggagaggt
      481 gcttgccgcc ggtgcggtag cgcgcgatcg agtaggcggc cgccgtcccg accaccgtcg
      541 ccgcaaccgt cgtgatcgac gcgatgatga gagagttctt cagccccttc cagccgttga
      601 acctgttcaa ggcctccgcg tagtgcagat cggtcagcgt gtccggccac cagatgggag
      661 gcgtcgcgag caggtcttcc tgtggcttga tcgaggccac gaacacccag tacagcggga
      721 cgagcaggaa gagcaggtag gcgccgacca ccagataccg cagccagccc accctcaacc
      781 gccagccgag gatgatcttg gccgagtcct ccgactcccg tcgcggttgc gcagcctcgc
      841 tcatgtcacg acccggcggc taccacggtc acgagcgcct cgctgatcgg cccctggctc
      901 cgaccgtcga tggcgacctc cgccgtcatc agccgacggc ggctgtcgcc gctaccgggc
      961 gcccgagccc gcagagcgag ctcgccgcga ccaccggcgt cgagagcgag ctcgcccgtt
     1021 ccgtcggcgg atacccatcc atccggcgca aggagccggg ccgagtacgt cgccgggcgc
     1081 tcgaggttgt tgcgcagcag gagccgatag acgacctcgt cgcccggtgc gaccgttgcg
     1141 acgtacggca gcaagcgcgc ccagaaaaga tcgacgtagt ggtcggccgg agccgacacg
     1201 agcccccgga acaccctgtc cttctcgtcg atgaacgcgc aatagtcgtc gagagccgcg
     1261 tcggagcact cgagcaccgg cccgtgcccc ggacagacca gatcggggcc gatgctgcgc
     1321 atgacctcgg cacaacgatg gtgcatgtcg agctggaagc tgttgcgcag caccgtcgtc
     1381 tgatacggga ggacgaccgc gctgccgccg cgcacgacct cgtcctccat gtagttgtca
     1441 ccggtgaaag ccaccgtgcg accgtcgacg accgtggcga tcaccgagtg gaactcggtc
     1501 tgccccggag cgtggtgaat ctcgagctcg tactcctccc acgtgaaccg ctcgccgttc
     1561 gccaggcgtc tgtcgacccg gatcggcttc gggtacacgc acggcgtgct cgcccaggcg
     1621 gacgggttct cgatcacctc tgccactatg tcaagggccc agcattcggt gccgtggaac
     1681 ttctgcaggt aggggatccc gcacgtgtga tcgtcgtgga tgtgcgtcgg caccacgaca
     1741 tcgatctcct tcactccgtg gtcgtcgcgg agcgtgtcga gccggtgctc gacgaagcga
     1801 agggtctcga ggccgccgtg gtcggcatgg atgtgcaggt gcgcgagatt cgccgtgccg
     1861 tagtcgaaga agagagcctt gccgctgtcg ctgagcagaa cgtagaagtt cgagcaggtc
     1921 gactccccac cccagagcag atgcggcgag agcggataca gctccagctt cggcaggtag
     1981 tccgcctcgg ggacgttcca gatgccgccg atccgcatgc cgcggcccag gcggacgcag
     2041 tccatgatcc cgttctcgag ccgcgagatg tcagcgtcga catccgtgat cgggtcgccg
     2101 tgggagggca gcgcgagcga cacctcctgc ctgcgcagcg cctggatcga ctggagcgtg
     2161 aacacgagcc cctcgagccc gctgtacatg tactccatcg cgtggagctg gtagagcgtg
     2221 cccccggcgc tcatgaggtc gccggtgaac gcgaccttct ggccgtcgat ctcggcgatc
     2281 agcgacgagg agccgaacgt gtggcccttg gccggcagca cgaggaagcg gtggccgcgc
     2341 cattcgaact cgtcgtagtc ctcgagcacc gcgtcgacgg cgacgctctg cccgagcgtg
     2401 aagaacgtgt tccggtcgtc gtagttgtcg aacacgcggc gtgtctgcca gaactcctcg
     2461 acccggtcga agaggtgacg ctcgtactcg ggcacagcga cgcgtgcgcc cgactgggcg
     2521 aggcgctcgg tgccccagca ctggtcgcgg tgatggtgcg tgtggaggat ccactgcacg
     2581 ctctcgacgc cgatctcggc gagccggccc tcgacggcgc cggagcccgc gtcgatcagc
     2641 agcgcggcgt cgccgtcgcg gatcacgtag acgttgcagg tgtcggccag gcaaaacagg
     2701 ttctccgaca gttgcgtcag ctgagtgctg tgcatcgact cctccttccg cggagcccgt
     2761 attctcgcgg aggccgtcgg tcgtggcacg cctgggaccc ccgaggtgcc acccggagtg
     2821 ggtcgcgact gcgatcatct ctcgcatgac gcctgacgtg gtcatcgtcg gcggcggcgc
     2881 gataggcgct acgacggcgt gggagctcgc acagcgcggc atggccgtga cgctgctcga
     2941 gcgtggcccc acgctcgcag cagggtgctc cgccggcaac gccggcctta tctgtccgag
     3001 tcattctgcg cccctcccca acccggcggc gctgcgaaac ggcctgcgct ggatgctgac
     3061 gccggacagc cccctgtacc tgcgaccgcg tccgggcgtg gcgccctggc tcgtccgctt
     3121 cgtcgccgcg tgccgccccg ccagagcgga ggcgggcacg cgcatcatcc gcgagctcag
     3181 catcgcgagc ctcgagctgc acgcacggat cgccgagcag ggcctcgaga ccggctttga
     3241 gcgacgcggg acgctgaacg cctacgagtc cgacgagggg ctacgccagg cgaggatcga
     3301 ggccgagcac ctcggcctcc ccacgcgtgc cctcgaccgc aaggagatcc acgcgctcga
     3361 gccggcgctc tcggagagcg tgataggcgg cgtgcactac gtcgacgagg cgcactgcga
     3421 gccgatcggg tacgtccagg ccgtcgcgga cgcagcggcc gacgccgggg ccgagatccg
     3481 aaccggcgtg gaggtcacgg cgctgcgacg cagggacggc cgggtggccg tcgtcacggc
     3541 cgagggcgag atcgcgcccg agacggtgat cctggccgcc ggcgcgtgga cctcgcagct
     3601 cgcgcgacag gtcggcgtct tcctcccgct cgagggaggc aagggctatc acatcgacct
     3661 cgagccggtg ccgggtgacc cccgggtgcc cgtattcgtg caggaggcgc ggacgatcgc
     3721 gacccctctc tccgaccgtc tccggctctc tggaacgctc gagctggcgg gcctcgacct
     3781 cacgatcagc ccgcgcagag tcgacgcgat cagacgcgcg atcggccgcg tgctgcgctt
     3841 ccaggaggac cggaaggtga tggaggtctg ggccggcctt cgaccctgca cgcccgacgg
     3901 cctgccggtg atcggccgcg tgccgggcct cgatggactc gtgctcgtga ccggccacgc
     3961 gatgaagggt ctctcgctgg cccctatcac cggtcgcctg gtgtcccagc tcgtaggcgg
     4021 cgaggagccc gacttcgatc tcgcgccgct ccgacccggg cgctttcggc ccctgctccg
     4081 gcgggccggc ctcaggcgca ccggcgcgcc gcgctcggca gcccagaacg cacgcccgac
     4141 gtagcgatga agtctgccgg gatgcgcatc tgctcgctct tgccgagcgc gaccgagatc
     4201 gtcggcgagc tagggatggc cgacaggctc gtcggccgct cggccgagtg cgactggccg
     4261 cccgaggtgc gcgagcttcc ggtcgtgacg gcttcccgag tcgacacggc cacggtcgcc
     4321 agctcgacga tcgacgatgc cgtgcgggct tcgatcgccg acggacgctc gctctacgcg
     4381 ctcgatcacg agctgatcaa ccagctcgcg ccggacttcg tgattacgca ggatctctgc
     4441 gcggtctgcg cggtctcgag cgcggacgtc ggggagctct gcgcggtcga cgccgaggtg
     4501 gtctcgctcg acccgccaac gctcgccggc atcgagcaga gcgtcgccga gctggcccga
     4561 cgactcgacg cgcccgagtc cggcgccgag gtcgtcgcac gcatgcggca gacgatcggc
     4621 cgggtacgcg agctcgtcga gggtcgtccg cgtcctcggg gctttgtcgc tgagtggctc
     4681 gatccgccct tcgccgccgg gcactgggtg cccgagatgg tcgcctcggc aggcggcgag
     4741 gagattctcg gccgcgccgg gaaggcgtcc ttcgcgacgt cgtgggaggc ggtgcgcgac
     4801 ggctcgccgg agctcgtcgt cctagctcca tgcggtttcg atgcggagcg ggctcgtgac
     4861 gaggcggacg ccctccctcc gctcgcgtgc cgaactgtcg ccgtggacgc caactcgtac
     4921 ttctcgcgcc cggcgccgcg cgtggccgac ggtgtcgcgc agctcggctt
//
LOCUS       DBNGNK010000261         4546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_190725,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000261 DBNGNK010000000
VERSION     DBNGNK010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_190725"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2863)
                     /locus_tag="AC150V_07705"
     CDS             complement(<1..2863)
                     /locus_tag="AC150V_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3762845.1"
                     /translation="MVHPGKRFCGACGERLRAEGWVGDATRHRQASVVALAGSRPGSA
                     SLAALHAVADDLEGVVVHRSGGVACVFGARRSLEDHLHRALIAALRLRERPGSAASAG
                     FGVASGRVPSDTGTSPDDPGADDPVAVAISLALTAGPNEALVDPALIPLVPDAIVVEP
                     AWTDGPGSGRDGAEEQPFRVIGTRTGPRPGRRQESRPLSRFVGRERQLRLVEEAITLC
                     ASGSGQVIGVVGEAGMGKSRLVEEVHRAVATRARWIEGRCAPFGTNHPYLPVLDLVRE
                     LCGVSQPDRPGVADAVTQTCARLEMPAGEASFVRHVLGALDGDDAAIAALTPKAVRIR
                     TFEALRHMFLAAARLDTIVIAVEDLHWADDTSQRFIASLAPAIAGARVLLLATYRPGR
                     RPPWLDLSHATQLTLPRLGPSDSLEVVRSVRDGEEVPEDLGRAILTRGDGNPFFLEEL
                     ALANPGGTTGAEVVPRTVEDVLTARIDRLDDGPRAVLQNAAVLGRRLTLRLLAAVSTA
                     AAPLPEYLEELEREEFLIGAVVDGEPGYEFKHALTQDVAYQSVSPTGRRELHEVAGLC
                     LEDFYAGRTDEVDGLLAHHWSRTDDAMRAVAYLRRAADRSFRGYAHLEASAMLREAMR
                     HAERLPVAERDRRVVELSLDLVASIYFLGRMTESEEILASLAPRVASASDPGLACAFH
                     FWAAHTASHLGKPDDAERDARSAIDLAPPLGDGAALGRALYIRARQGWWTGRFEEGVR
                     AGEEAVPLLEHAGETWWLGHCHFFVAHSLYSMGRFDAALDAAARGGAIGDAVADPRLR
                     SWAAWAQGLYEAARGDTERGVAQCLRGIELSPDEPNTAWALGALGFSRREHGDLEEAI
                     DDLQRAIDIAERTNHPGILARFLGWLGEARRRAGDLAGASEATTRSGRIGGEIGCRWV
                     VALALRTRGRIAFDRGDLVAARRLLGEARAALEAIGSTFD"
     gene            complement(2885..2974)
                     /locus_tag="AC150V_07710"
                     /pseudo
     CDS             complement(2885..2974)
                     /locus_tag="AC150V_07710"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3359..3583
                     /locus_tag="AC150V_07715"
     CDS             3359..3583
                     /locus_tag="AC150V_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762846.1"
                     /translation="MSDPCDLPGLDGSVGSPSAGATFAAYSAARRRFLETLHQDSEMR
                     RLEAAWSLPDEPAGGRFEASRPPDVTSTGL"
     gene            complement(3695..4168)
                     /locus_tag="AC150V_07720"
     CDS             complement(3695..4168)
                     /locus_tag="AC150V_07720"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008127 - quercetin 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HIR3762847.1"
                     /translation="MTTLQAPPALLGPLEGEAHWWTGELATIKATGAETDGHLALVEI
                     LAPDGIAIPMHVHRNEDEGFWVLEGEVTFQMAGRRIAARPGDFVLGPRGVPHAYAVDT
                     GPARMLFLFTPAGLEGFIREAGVPAAERALPPPDVQPDWDAIPALAARYGVELIG"
     gene            4253..>4546
                     /locus_tag="AC150V_07725"
     CDS             4253..>4546
                     /locus_tag="AC150V_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR3762848.1"
                     /translation="MGRRRYDMSKRAATIERTRRRILEATMEVHDEQGIVDGRWPDIA
                     ERAGVSLATVYRHFPSLEDLALASGELTMEVVGPPSPETAAEVFRGARSRAARI"
BASE COUNT          653 a         1647 c         1642 g          604 t
ORIGIN      
        1 ggtcgaacgt gctcccgatc gcctcgagcg cggcgcgggc ctcgccgagc agccggcggg
       61 ctgcgaccag gtcccctcga tcgaaggcga tccgtccgcg cgtccgcagc gcgagggcga
      121 cgacccagcg gcatccgatc tcgcctccga tccggccgga gcgtgtcgtg gcctcgctcg
      181 cgccggcgag gtcacccgca cgtcgtcgcg cctcgcccaa ccagccgagg aagcgcgcca
      241 ggatcccggg gtggttcgtc ctctcggcga tgtcgatcgc ccgctggagg tcgtcgatcg
      301 cctcctcgag gtctccgtgc tcgcgacggg agaacccgag ggcgccgagc gcccaggccg
      361 tgttcggctc atcgggagac agctcgatcc cgcggaggca ctgcgcgact ccgcgctcgg
      421 tatcgccgcg tgccgcctcg tagagcccct gggcccaggc cgcccagctt cggagccgcg
      481 gatccgcgac cgcgtctccg atcgcgccgc cccgggcggc cgcgtcgagg gcggcgtcga
      541 accgtcccat cgagtagagg gaatgggcga cgaagaagtg gcagtgcccg agccaccagg
      601 tctcgcccgc gtgttccagg agtgggacgg cttcctcccc ggcccgcacg ccctcctcga
      661 acctcccggt ccaccatccc tgccgggcgc ggatgtagag cgcccgtccg agggcggccc
      721 cgtctccaag tggaggggcg aggtcgatcg ccgaacgggc atcgcgttcg gcgtcgtccg
      781 gcttgccgag gtggctcgcc gtgtgggccg cccagaaatg gaaagcgcac gcgagacccg
      841 ggtcgctcgc gctcgccacg cgcggcgcga gagacgcgag gatctcctcg ctctcggtca
      901 tgcggccgag gaagtagatc gaggcgacca ggtccaggct gagctcgacg acgcgtcgat
      961 cgcgctcggc gacggggagc cgctcggcgt ggcgcatcgc ctcgcggagc atcgcgctcg
     1021 cctcgaggtg cgcataaccg cggaagctcc gatccgcggc tcgtcggaga taggcgaccg
     1081 cgcgcatcgc gtcgtccgtg cgagaccaat gatgcgcgag caggccgtcg acctcgtcgg
     1141 tccggccggc gtagaagtcc tcgaggcaga gcccggcgac ctcgtgcagc tcgcgtcgcc
     1201 cggtcgggct gacgctctgg taggcgacgt cctgggtgag cgcgtgcttg aactcgtatc
     1261 ccggctcgcc gtcgacgacc gccccgatca ggaactcctc ccgctccagt tcctccaggt
     1321 actcgggtag cggcgccgcg gcggtggaga ccgcggcgag aaggcggagc gtgagccgcc
     1381 ggccgagcac ggcggcgttc tggaggacgg cgcgtggccc gtcgtcgagc cgatcgatac
     1441 gggccgtgag aacgtcctcg accgtgcgtg gcacgacctc ggcgccggtg gtgccgcccg
     1501 ggtttgcgag cgccagctcc tccaggaaga acgggttgcc gtccccccgg gtgaggatcg
     1561 cgcgtccgag atcctccggg acctcctcac cgtctcgcac ggagcggacg acctcgagcg
     1621 agtcggacgg cccgagccgt gggagcgtga gctgggtcgc gtgggacagg tcgagccacg
     1681 gtgggcggcg accgggccgg taggtcgcca gcagaagcac ccgtgcgccg gcgatcgccg
     1741 gagcgaggga ggcgatgaac cgctgcgagg tgtcgtcggc ccagtgcaga tcctcgaccg
     1801 cgatgacgat cgtgtcgagg cgggcggcgg ccaggaacat atggcgaagt gcctcgaagg
     1861 tacgtatgcg aaccgccttg ggagtcaggg cggcgatcgc cgcgtcgtct ccgtccaggg
     1921 ctccgaggac atgtcgtacg aatgaggcct cgccggcagg catctcgagg cgtgcgcacg
     1981 tctgcgtcac ggcgtcggcc acaccgggcc gatcgggctg cgagaccccg cagagctctc
     2041 ggaccagatc gagcacaggc aggtacgggt ggttggtgcc gaaaggtgcg cagcggccct
     2101 cgatccagcg cgcgcgcgtg gcgacggcac gatgcacctc ctcgacgagc cgcgacttgc
     2161 ccatcccggc ctcgccgacg actccgatga cctgccccga ccccgatgcg cagagggtga
     2221 tggcctcctc cacgagccgc agctggcgct cgcgcccgac gaacctgctc aacggccggc
     2281 tctcctgacg acgccccggc ctcgggccgg tgcgggtgcc gatcactcgg aacggctgct
     2341 cctctgcacc gtcgcggccc gacccgggcc cgtcggtcca ggccggctcc accacgatcg
     2401 cgtcgggaac cagcggaatc agcgccgggt cgaccagagc ctcgttcgga ccggccgtga
     2461 gggccaggga tatcgcgacg gcgaccggat cgtccgcgcc cggatcatcg ggagaggtcc
     2521 cggtgtcgct gggaaccctc ccggatgcga ccccgaagcc ggccgacgct gctgaccccg
     2581 ggcgctcccg gaggcgcagg gccgcgatga gggcgcggtg cagatggtcc tcgagggaac
     2641 ggcgggcccc gaagacgcac gcgaccccac cggagcggtg cacgacgacc ccctcgaggt
     2701 cgtccgcgac ggcgtggagc gccgcgagcg atgcgctccc cgggcggctc ccggcaagag
     2761 cgacgacgct cgcctgacga tgccgcgtcg cgtctccaac ccacccctcc gccctcaagc
     2821 gctcgccgca tgccccgcag aaccgcttgc cgggatggac catggctccg catgaggagc
     2881 agcgcgcagg gatcgcgcca ccgcagtcct cgcagtagcg ggcgcccact cggggcgctg
     2941 cgccgcaggc cgggcatggg gcggccgtgc tcatgccgcg ctcgtcctcc cgccgtcgcg
     3001 cgaggtcaga tcgcggagac cacagccctt cgcgtcggcc aacacgcgcg tcgtcacgcc
     3061 gcgagctccc gggaatccga cactcccatc gaacagtggg cgggtcaacc cgtccccgtc
     3121 cggcggcgtc tacctacacg gtgagggcga cacgccggcg accgccgtcg agatcgaaag
     3181 cacttgggat gcgcctcccg ggccgctgct cgagcatcgg gtcccaccag cgtctcgggc
     3241 gggagtcacc gttgccgctc tcatccgtgc gtcgcacctc cacctcggcg tccatcgggc
     3301 cgaacgcgac gcgctcgacg gggtcaccac ctacacagct cgtcccgagg ggtgaatcat
     3361 gagcgatccc tgcgacctcc cgggcctcga cggctccgtc gggtcgccga gcgccggagc
     3421 gacgttcgcg gcctactcgg cggcccgccg gcgtttcctc gagaccctgc accaggactc
     3481 ggagatgcgg cggctcgagg ccgcgtggtc actccctgac gaacccgcag gcggacgatt
     3541 cgaggcctcg cgaccgcccg atgtcacatc gacggggctc tgatctcggc ctcacgggtc
     3601 ggacctcacg cgggcacgag gcgttcgggt gctcgtcggg acgaggccga ccacgagggc
     3661 gccggggcga tgctcggacc ggccccggag cccgtcagcc gatcagctcg acgccgtagc
     3721 gggcggcgag ggccgggatc gcgtcccagt ccggctggac gtcggggggt ggcagggccc
     3781 gctcggcggc cggcacgccg gcctcgcgta tgaagccctc caacccggcc ggggtgaaca
     3841 ggaacagcat ccgggccggt ccggtgtcga cggcgtaggc gtggggcacg ccgcgcgggc
     3901 cgagcacgaa gtccccggga cgggccgcga tccgccgccc ggccatctgg aaggtcacct
     3961 cgccctccag gacccagaag ccctcgtcct cgttgcggtg cacgtgcatc gggatcgcga
     4021 tgccgtcggg ggcgaggatc tcgaccagcg ccaggtggcc gtcggtctcg gcgccggtcg
     4081 ccttgatcgt ggcgagctct ccggtccacc agtgggcttc tccctcgagc gggccgagca
     4141 gcgccggcgg cgcctgcagg gtggtcatcg gtgcgtctcc tttgtcgtcg gtgaccgatg
     4201 gcggtacgat gattcatgag aaacatattg tcaagaaaaa ggttggcgcg aaatgggacg
     4261 ccgtcgatac gacatgagca agagggccgc gaccatcgaa cgcacgcgcc gtcgcatcct
     4321 cgaggccacg atggaggtcc acgacgagca agggatcgtc gacggccgct ggccggacat
     4381 cgccgagcgc gccggtgtct cgctggccac ggtctaccgg cacttcccga gcttggagga
     4441 cctggcgctc gccagtggcg agctgaccat ggaggtcgtc gggccgccga gcccggagac
     4501 ggcggccgag gtcttccgcg gcgcacgctc gcgcgccgcc cggatc
//
LOCUS       DBNGNK010000262         4522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1335281,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000262 DBNGNK010000000
VERSION     DBNGNK010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4522
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1335281"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="AC150V_07730"
     CDS             complement(<1..586)
                     /locus_tag="AC150V_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR3762849.1"
                     /translation="MPRIGVFGAGYVGLVTGACFADLGHEVVVRDVLPERIAQLRRGE
                     VPIYEPGLADVIERNAKRLSFTLDVGEAIEGADFVYIAVGTPPTYSGDADLSAVWTVI
                     DELPAIDRRVTVVTKSTVPVGTGARVRRRLDARGLRHVGYASNPEFTAEGTAVRDFMR
                     PDRIVVGAFDETDAAAVSGLHSGIDAPVVRCEVAS"
     gene            complement(598..2454)
                     /locus_tag="AC150V_07735"
     CDS             complement(598..2454)
                     /locus_tag="AC150V_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR3762850.1"
                     /translation="MTLYRQPEPPARSRTSLALRILGWAVVALAMCVGGVAGGVYLYL
                     HESVAAVAPKSEEVRRAARQLNTPLPNEATTALVIGFDRRKGDPKGTPFRSDTLMLVR
                     ADPSAKTISMLSFPRDLIAEIRCPGREPYNDRINAAFTQCGPRGALETVKGVTGLPVN
                     YLITVNFRGFREIVDRLGGVWMDIDRRYFNDRGGPGGYATINIQPGYQRLNGWRALDF
                     VRFRHTDSDIHRNARQQLFVRAVKEQLESSFSLTTLPKLINTVTKNVEVAQGGGKDVS
                     AKTVLGYALLAYSLPSGHVFRSQIDGLEGFAELRAPPESIQRAVRQFLHPDVEAPKKA
                     TRVALGEKPKSAQAPPPPKDTTVAVLNGNGVTGSATNAGFLLGQRGYQILTPPNGVPA
                     NAPNFEYFRSKVYFDPKRPGAQLAAKKVANLFGSADVGRLNGAIKQLSGGAMLVTVVG
                     QTFHGTLASAPIDQTPKKQEAAVSPGTSAAVDHLRERAGRVSFPMMVPTVIERSSWID
                     PEKPIRMYWMDEKKKHKAVRLTYRTGAGNEYWGVQMTDWGDAPVLSGRSFVRNIAGRR
                     YELYFNGPKLHMVVLRTDKATYWVVNTLLDRLSNETMLAIAKGLRPIGKVKQ"
     gene            complement(2614..3735)
                     /gene="hisC"
                     /locus_tag="AC150V_07740"
     CDS             complement(2614..3735)
                     /gene="hisC"
                     /locus_tag="AC150V_07740"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR3762851.1"
                     /translation="MSLGDVDAGLLRPALRDIVPYEPGKPVEEVQRELGLAHVVKLAS
                     NEGPYGPFPAAVEAIERATRELNRYPDGGAFRLRHALAEYHGVRFEEVTVCAGADAVI
                     GYLALATLDPGDEIVTGWPSFPSYVLDALKLGAAPVRVPLRDDRFDLDAILASVTPKT
                     KLVFLANPNNPTGTMNSRAQLDAYFDAVPQHVLTVLDQAYFEYIDDPDYPDGIEEYAK
                     TGRHVVVLRTFSKIYGLAGLRVGYGIAPAQIVTAIGKVRRAFDVTSAGQEAALASLGA
                     EDELARRRTTNRQAMRSLEDALRRRGYAPAGPAVANFLFVEVGDDAAAFNDALLRRGV
                     IVRPMGPFGAPGALRITAGTPDEIAYLAQQLAEIVPAAR"
     gene            complement(3732..4082)
                     /locus_tag="AC150V_07745"
     CDS             complement(3732..4082)
                     /locus_tag="AC150V_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762852.1"
                     /translation="MFNRRNAIIGFLVVKILKRGAKRKLPTPSLPFTARKNERPWGRW
                     LVIGSAGVLVGAIVGGILWRRRSSGQIDFAEFEYSETAFEELVPPDVPVADELEGDAA
                     DDAASAETADSPAE"
     gene            complement(4142..>4522)
                     /locus_tag="AC150V_07750"
     CDS             complement(4142..>4522)
                     /locus_tag="AC150V_07750"
                     /inference="COORDINATES: protein motif:HMM:NF014860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR3762853.1"
                     /translation="TDPEIAGVGLTEAEAREEYGDDNVAAGVFPWLANARAVMSGDAN
                     GWVKSIHETTYGELLGIVIVGPHATDLIGAGVIALDAEATVETVADGIAPHPTLAEAV
                     KEAGLVALGRPIHIPAKRRAAAKA"
BASE COUNT          674 a         1548 c         1557 g          743 t
ORIGIN      
        1 cggaagcgac ctcgcagcgg acgaccggcg cgtcgatccc gctgtggagc cccgagacgg
       61 ccgcggcgtc ggtctcgtcg aaggcgccca cgacgattcg atcgggtcgc atgaagtcgc
      121 gtacggcggt tccttcggcg gtgaactccg gattcgacgc gtagccgacg tgacgaagac
      181 cgcgtgcgtc gagccggcgt cgaacgcgcg ccccggttcc caccggaacg gtgctcttcg
      241 tcacgacggt gacgcgccgg tcgatcgccg ggagctcgtc gatcacggtc cagacggcgg
      301 acagatctgc gtcgcccgag taggtgggcg gcgttcccac ggcgatgtag acgaagtctg
      361 ctccttctat tgcctccccg acgtcgaggg tgaacgacag ccgcttggcg ttccgttcga
      421 tcacgtctgc gaggcccggc tcgtagatcg gaacctcacc gcgccgcaac tgcgcgatcc
      481 gctccggcag cacatctctg accacgacct cgtggccgag gtcggcgaag cacgcaccgg
      541 tgacgaggcc gacgtagccg gcaccgaaca ctcctatcct tggcacggcg ccgaagccta
      601 ctgcttgacc ttgccgatcg gccgcaggcc ctttgcgatc gcaagcatcg tctcgttcga
      661 gagcctgtcg agcagcgtgt tcacgaccca gtacgttgcc ttgtcggttc gtaggacgac
      721 catgtgcagc ttcggcccgt tgaagtagag ctcgtaccgg cgacccgcga tgttgcggac
      781 gaagctcctg ccgctgagta ccggcgcgtc gccccagtcg gtcatctgca ctccccagta
      841 ctcgttccct gccccagtgc gatacgtgag ccgaacggcc ttgtgcttct tcttctcgtc
      901 catccagtac atgcggatcg gtttttccgg gtcgatccac gacgagcgct cgatcaccgt
      961 cggaaccatc atcgggaagg agacgcgccc ggctcgctcc cgcaggtggt cgacggcggc
     1021 cgaggtgccc ggcgagacgg cggcctcctg cttcttcggc gtctgatcga tgggcgcgga
     1081 ggcgagcgtc ccatggaacg tctgtccgac caccgtcacg agcattgccc cgcccgacag
     1141 ctgcttgatc gcgccgttca gcctccccac atcggcggag ccaaagaggt tcgcgacctt
     1201 cttggcggcg agctgtgcgc ccggacgctt cgggtcgaag tagaccttgc tgcggaagta
     1261 ctcgaagttg ggagcgttcg cgggaacgcc gttgggcggc gtcaggatct ggtagccgcg
     1321 ctggccgagg aggaaccccg cattcgtcgc cgaccccgtc acgccgttgc cgttgaggac
     1381 ggccaccgtc gtgtccttcg gaggcggcgg agcctgcgcg ctcttcggct tctcgccgag
     1441 agcgactctc gtcgccttct tgggtgcctc gacatcgggg tgcaggaact gacgcacggc
     1501 gcgctgaatg ctctcgggcg gggcccggag ctcggcgaag ccttcgaggc cgtcgatctg
     1561 gctccggaac acgtgcccgg acgggaggct gtaggcgagc agcgcatagc cgaggacggt
     1621 cttcgcgctg acgtccttgc cgccgccctg ggcgacctcg acgttcttcg tcaccgtgtt
     1681 gatcagcttc ggcagcgtcg tgagcgagaa cgagctctcg agctgctcct tgaccgcgcg
     1741 gacgaagagc tgctgccgcg cgttccggtg gatgtccgag tcggtgtggc gaaagcggac
     1801 gaagtcgagc gcgcgccagc cgttgaggcg ctggtagccg ggctggatgt tgatcgtggc
     1861 gtagcctccg ggccctcccc gatcgttgaa gtagcggcgg tcgatgtcca tccagacacc
     1921 gccgagccgg tcgacgatct cgcggaagcc gcggaagttg accgtgatca ggtagttgac
     1981 cggcagcccc gtgacgccct tgacggtctc gagggcgccg cgcggcccgc actgcgtgaa
     2041 ggcggcgttg atcctgtcgt tgtacggctc tcggccgggg cagcggatct cggcgatcag
     2101 gtcacgcggg aacgagagca tcgagatcgt cttcgcggac ggatcggcgc gcacgagcat
     2161 gagggtgtcg gagcggaaag gagtgccctt ggggtcgccc ttccgccggt cgaagccgat
     2221 aaccagagcc gttgtggcct cgttcggcag cggcgtgttg agctgccggg cggcgcggcg
     2281 aacctcctct gacttcggcg ccacggcggc gacggactcg tgcaggtaga ggtagacgcc
     2341 acccgccacc ccgccgacgc acatcgcgag ggccacgacc gcccagccga ggatccggag
     2401 cgcgagcgac gtccggctcc gcgccggcgg ctcgggctgc cggtacagag tcatcgggga
     2461 cagctgaccg ggcggcaaca ccggcttgcc gtttccattc gcggcgtggc cgctcccgat
     2521 tcctcgtttc agagtggttc gcatgcggcg cagggcgcca ccgcgcccga tcgcccagga
     2581 tagctgaggg tccggccgcc tcggctgctc gcgctagcgc gctgccggaa cgatctcggc
     2641 gagctgctgg gccagatatg cgatctcgtc gggcgttccc gcggtgatcc gcagagcgcc
     2701 cggcgcaccg aacgggccca tcgggcgcac gatgaccccc cggcggagca gcgcgtcgtt
     2761 gaacgcggcc gcgtcgtcgc cgacttcgac gaagaggaag ttcgcgacgg ccggccctgc
     2821 gggcgcgtac ccccgccggc gcagcgcgtc ttcgagcgag cgcatcgcct gccggttcgt
     2881 cgtgcgtcgt cgtgcgagct cgtcctctgc cccgagactc gcgagtgccg cttcctgtcc
     2941 tgccgaggtg acgtcgaagg cgcgccgcac cttccctatc gcggtcacga tctgcgcggg
     3001 cgcgatcccg tagccgacgc ggagcccggc gaggccgtag atcttcgaga acgtccggag
     3061 gacgacgacg tgccggccgg tcttcgcgta ctcctcgatg ccgtcgggat agtcagggtc
     3121 gtcgatgtac tcgaagtagg cctgatccag cacggtgagc acgtgctggg ggactgcgtc
     3181 gaagtaggcg tccaactggg cgcggctgtt catcgtcccc gtcgggttgt tcgggtttgc
     3241 caggaagacg agcttcgtct tcggggtcac cgaggcgagg atcgcgtcga ggtcgaaacg
     3301 gtcgtcgcgc aacggaacgc gcaccggcgc agcgccgagc ttgagggcgt cgagcacgta
     3361 gctcgggaac gacggccagc cggtgacgat ctcgtcgccc ggatccagcg tcgccaaggc
     3421 gaggtagccg atgacggcgt ctgcgccggc gcacaccgtg acttcctcga agcgcacgcc
     3481 gtggtactcg gcgagggcgt gccgaagccg gaacgcgcca ccgtccgggt agcggttcag
     3541 ctcccgcgtc gcacgctcga tggcctcgac cgcggccggg aacggcccat agggcccctc
     3601 gttggaagcc agcttgacga cgtgcgcgag gccgagctcg cgctggacct cctcgaccgg
     3661 cttgccgggc tcgtacggga cgatgtcgcg cagcgcgggc cgcagcaggc ctgcgtcgac
     3721 gtcgccgagg ctcactcggc cggcgagtcc gcggtctctg cggaagccgc gtcgtccgca
     3781 gcgtcgcctt cgagctcgtc tgcgacgggg acgtccggcg gaactaactc ctcgaaggcc
     3841 gtctcggagt actcgaactc ggcgaaatcg atctggcccg agctgcgacg ccgccacagg
     3901 atcccaccga cgatcgcccc caccagcaca ccggcggacc cgatcacgag ccagcggccc
     3961 cacgggcgct cgttcttgcg cgcggtgaag ggaaggctcg gcgtcggcag cttccgcttg
     4021 gcgccccgct tcaggatctt gacgacgaga aagccgataa tcgcgtttct tcggttgaac
     4081 atggccccgg gcagcctatc gcgcacgggg cggcccatgc gggaacggct tccgcagccg
     4141 atcaggcctt ggctgcggca cggcgcttcg ccggaatgtg gatcggccgt ccgagcgcaa
     4201 cgagacccgc ctccttgacg gcctcggcca gggtcggatg aggcgcgatc ccgtccgcga
     4261 ccgtctccac cgtcgcctcg gcatcgagcg cgatcacgcc ggccccgatc agatcggtgg
     4321 catgaggccc gacgatcacg attccgagca actcgccgta cgtcgtctcg tgaatcgatt
     4381 tcacccagcc gttcgcgtca cccgacatca ccgctcgagc gttggcgagc caggggaata
     4441 cgcccgcggc gacgttgtcg tcaccgtact cctcacgcgc ctcggcctcg gtgaggccga
     4501 cacccgcgat ctccgggtcg gt
//
LOCUS       DBNGNK010000263         2550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1910674,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000263 DBNGNK010000000
VERSION     DBNGNK010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1910674"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="AC150V_07755"
     CDS             complement(<1..273)
                     /locus_tag="AC150V_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014059599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR3762854.1"
                     /translation="MADDLRVRLGQELGPTSWLEVSQDRIDAFAAATDDQQWIHVDRE
                     RAAAGPFGTTIGHGFLTLALCVPMLYEVLPPSRRTAINYGVNRVRFP"
     gene            324..1322
                     /locus_tag="AC150V_07760"
     CDS             324..1322
                     /locus_tag="AC150V_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3762855.1"
                     /translation="MSRPEVEFHVGLLPDRPVPETAELAAHAESLGFAGAWLADSQSV
                     MRDAYVTLAAAASRTARIRLATGVTNPVTRHCAVIADAIATIDELSRGRAVLGIGVGE
                     SSVRTLGLEPASLRELEQATTSIRSLLAGDEIELDGATTRLSWPTRPVPIWLASSGPR
                     SLRLAGRIADGVLFQVGSHPQLVRWALARIHEGVVAGGRNPASVGRLVRLACSVHDDR
                     EWARDQVRGYVAGAAGTVFWAVPEGEMPPGLHTAIAAMKKRYDYFEHMRSDAGHRELV
                     TEEMVDAIAIAGTPEEAVPRFRELIALGVDGFVLPITTGDPVETMRILARDVIPQL"
     gene            complement(1339..>2550)
                     /locus_tag="AC150V_07765"
     CDS             complement(1339..>2550)
                     /locus_tag="AC150V_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762856.1"
                     /translation="LRARVIWQGDELPLRTAQARLAVLADYGDRDELGELQRAVSAGF
                     NDDRRSLLLARNELEGELTGETDAVKRNEHEKGIELRPLLRELEAVSARTTPAWEELR
                     ERWFDRLLGPEREERPASSHTAYMRRLSPLADTYTKERSVPVCVATLERLGFDLERTP
                     GIRLDLDDRPQKSPRACVIASDPPKVVHLITRAQGGLHDYQAFLHEAGHALHYAGCDP
                     SLPYTFRRIARDHALTEIYSYIVEAVSREPGWHAEHFGLSASDAETNAEATTFLEALL
                     YRRYTAKLGFELDFWTRFPTDGGTSDGYEQRLTQATGIRYPSSMYLSDMDAGFYSADY
                     LRAWIRSAQLQEYLRREVGEDWWRRSETGDLLRGLFWEGTRPSSEEIAARIDFEPLDT
                     APLAVELAASA"
BASE COUNT          367 a          885 c          900 g          398 t
ORIGIN      
        1 gggaaagcgc acgcggttga cgccgtagtt gatcgccgtt ctcctggacg gcggcaacac
       61 ctcgtacagc attggcacgc agagggccag cgtcagaaag ccgtggccga tcgtcgtccc
      121 gaacggcccc gccgctgcgc gctcgcggtc gacgtggatc cactgctgat cgtcggtcgc
      181 cgctgcgaac gcgtcgatcc gatcctgcga cacctcgagc cacgaggtcg ggcccagctc
      241 ctgccccagc cgcactcgca gatcgtcagc catcggtcgc tccttgtcgg gcgtcggcgg
      301 atgctccatg ctgccgcgac gtcatgtcaa gaccggaagt cgagttccac gtcggcctcc
      361 tgcccgatcg ccccgtgccg gagaccgccg aactggcggc gcacgccgaa agcctcggat
      421 tcgcgggcgc gtggctcgca gactctcaat cggtgatgcg ggacgcctac gtgacgctcg
      481 ccgccgccgc ctcccggaca gcgcggattc ggcttgccac cggggtgacg aacccggtga
      541 ccaggcactg cgcggtgatt gccgacgcta ttgcgacgat cgacgagctc tcgcgtgggc
      601 gcgctgtgct cgggatcggg gtcggcgaga gcagcgtccg cacgctcgga ctcgagcccg
      661 cgtcgctccg cgagctcgaa caggcaacca cgtcgatccg atccttgctc gcgggggacg
      721 agatcgagct cgacggggcg acgacgcggc tgtcctggcc gacccgcccg gtgccgatct
      781 ggcttgcgtc gtccggcccg cggtcgctcc ggcttgccgg gcgcatcgcg gacggcgtgc
      841 tcttccaggt cgggtcgcat ccgcagctcg tgcgctgggc gcttgcacga atccatgagg
      901 gcgtcgtggc cggcggccgc aacccggctt cggtcggtcg actcgttcgg ctcgcatgtt
      961 cggtgcacga cgatcgagag tgggcgcgtg atcaggtgcg tggctacgtc gccggtgcgg
     1021 ccgggacggt cttctgggcg gtgcccgagg gcgagatgcc gccgggcctc catacggcga
     1081 tcgcggcgat gaagaagcgg tacgactact tcgagcacat gcggagcgac gcggggcacc
     1141 gggagctcgt gacggaggag atggtcgacg ccatcgccat cgccggaacg cccgaggagg
     1201 cagttccgcg gtttcgtgag ctgattgcgc tgggtgtgga cggcttcgtc ctcccgataa
     1261 cgaccggcga tccggtcgag acgatgcgga ttctcgcccg cgacgtcatc ccgcagctct
     1321 gagccgggcg gacacgcttc aggccgacgc ggccagctcc acggccaacg gggccgtgtc
     1381 gagcggctcg aagtcgatcc gcgcagcgat ctcctcgctc gagggccgcg tgccctccca
     1441 gaagaggccc cgcagcaggt cgccggtctc gctgcggcgc caccagtcct cccccacctc
     1501 gcgccgcaag tactcctgca gctgcgccga gcgaatccag gcccgcaggt agtcggcgga
     1561 gtagaagccc gcgtccatgt cggagaggta catcgaggac ggatatcgga tcccggtcgc
     1621 ctgggtgagc cgctgctcgt agccgtcgga cgtcccgccg tcggtcggaa accgtgtcca
     1681 gaaatcgagc tcgaagccga gcttcgccgt gtagcggcgg tacagcagcg cctcgaggaa
     1741 cgtcgtcgcc tcggcgttgg tctccgcgtc cgaggcggag agcccgaagt gctccgcgtg
     1801 ccagcccggc tcgcgagaga ccgcctccac gatgtacgag tagatctcgg tgagcgcgtg
     1861 gtcgcgggcg atccgcctga acgtgtacgg gagcgacgga tcgcatcccg cgtagtgcag
     1921 cgcgtgaccg gcttcgtgca ggaacgcctg gtagtcgtgc agcccgccct gcgcgcgggt
     1981 gatcaggtga acgaccttcg gcgggtccga agcgatcacg cacgcccgtg gcgacttctg
     2041 cgggcgatcg tcgaggtcga gccggatacc gggagtccgc tcgaggtcga agccgaggcg
     2101 ctccagcgtc gcgacgcaca cgggcaccga gcgctccttc gtgtaggtat cggccagcgg
     2161 ggagagccgc cgcatgtagg cggtgtgcga ggaagcgggc ctctcttccc tctcgggccc
     2221 gagcagccga tcgaaccagc gctcgcgcag ctcctcccac gcgggggtcg tccgcgccga
     2281 gacggcttcc agctcgcgca ggagcgggcg cagctcgatg cccttctcgt gctcgttgcg
     2341 cttgaccgcg tcggtctcgc ccgtgagctc gccctcgagc tcgttccggg ccaggagcag
     2401 gctccgccga tcgtcgttga aaccggctga gacggcgcgc tgaagctctc cgagctcgtc
     2461 ccggtcgccg tagtctgcga gcacggccag ccgcgcctgc gccgtccgta atggcagctc
     2521 gtcgccctgc cagatcacgc gggcgcgcag
//
LOCUS       DBNGNK010000264         5739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_907419,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000264 DBNGNK010000000
VERSION     DBNGNK010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5739
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_907419"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC150V_07770"
     CDS             <1..252
                     /locus_tag="AC150V_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762857.1"
                     /translation="ARLRAAGAPNPDSVYKRLAERGPIALFEVSPVPFRPRLVLTLRP
                     RASSRYTDAFTTELSCPRQRDLDCFRRRAGETVTVTKLS"
     gene            complement(262..894)
                     /locus_tag="AC150V_07775"
     CDS             complement(262..894)
                     /locus_tag="AC150V_07775"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR3762858.1"
                     /translation="MELSADDPWVRRGYGQASISPGERPAVLVVDLQYAFTDPAFPFG
                     GAELIERATVNTARLTAAARRSAVPVFQTVVAWEGERDIGLWAVKLPACAEITPGSRW
                     AEVDERVWDAGDTLLTKKWPSFFHGTPLVSLLTAAGCDTVVVTGCTTSGCIRATTVDA
                     FSYGFRTLVPEDCVGDQGRDAHESNLRDVHRRYAEVTTCGEVVAYLEGLG"
     gene            complement(993..1433)
                     /locus_tag="AC150V_07780"
     CDS             complement(993..1433)
                     /locus_tag="AC150V_07780"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3762859.1"
                     /translation="MSADNKKLMQRLINEAVSKGNLEVVDEIVAPDWIDNDPANPPDL
                     PRGAEGLKHLISMYRGAFPDMTMTIEDQIAEGDMVASRWTVRGTHNGDLMGIAPTGKQ
                     VTLSGIFIDRISGGKIQESWANWDALGMLQQVGAVPTPEAAHAG"
     gene            complement(1521..2813)
                     /locus_tag="AC150V_07785"
     CDS             complement(1521..2813)
                     /locus_tag="AC150V_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HIR3762860.1"
                     /translation="MRFDLLIRGGEVVDPGGGNEGNLDVAITRGRVAAVDRDIPAEAA
                     FDVIDAEGLYVMPGLIDLHTHVFHKVTYWGINPDPFASRSGVTTWNDAGSAGALTLQG
                     LREFVADPARVSLTAFLNISDIGLVADNEECANLSYLDVGLFRRLADRNRDFVRGVKV
                     RIGSPTVGENGVEPLRRARQAAEECELPLMVHIAIGPPEIEDVLALMRPGDILTHCFT
                     GLTMKIVDERGQLYDFATRAWDAGVIMDIGHGTGSFSFETAEALMAAGRAPDVISTDL
                     HQLSAKGPAYDMPTCMSKFLHLGMSLRDVVQAVTSRPAEILGIEQEAGTLRPGSRADV
                     ALFRLHSGRFPVYDIHGTMRQSRVLLESTRTIVGGRTLAVLPPDPPAPWVEDPIWPEA
                     MQPFTAQQATLDELGHTPDAMIAAAAEDAESASTRPRS"
     gene            complement(3143..3985)
                     /locus_tag="AC150V_07790"
     CDS             complement(3143..3985)
                     /locus_tag="AC150V_07790"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3762861.1"
                     /translation="MTSDRAASTFDTYAATSIDDLLALDGRAAIVTGAAQGFGFAAAR
                     RLAGAGAAVLLTDRRPEAVEAAAGRLAHHGDGVSWAVGDVSVPEEIDGLVSAAVERFG
                     RLDVLVNNAAVYSNTRIHELPLDDFAGAIDVNVQGAFWCARQAARQMIQQGDGGAIVN
                     MTSIDALHPTSQGMAHYTTSKHALWGLTKCLALELGPRGIRTNAIAPGPSLTEGTIEY
                     IKAGAPEGIDLERQWDEASKRVPMRRWADPDEIARVVVFLASDLSAYINGAQIVVDGG
                     YLVG"
     gene            complement(3982..5289)
                     /locus_tag="AC150V_07795"
     CDS             complement(3982..5289)
                     /locus_tag="AC150V_07795"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3762862.1"
                     /translation="MEQRPAVAEAVAASREGLRDLLRDLIAFRTESQAKEAAHFPEEV
                     RGCVDYVCGFLSDLGFESERWDVGPSATFDAHPAVIARLPGSGGGRSLAFNGHLDVVP
                     VGDTSAWSEDPFGGALVDGRIYGRGATDMKGGVAAAMWAVKVALDQGFRPSGDIFFHV
                     VTDEEVVGNGTREIVERAPAADVTLSLEPTELQLCPAEGGLVHFRIEVEGIEAHASTR
                     YRAVHAGGKGGGGVNAIEKAIKIVVALQELERQWANTKSHAMLPPGHDTLSPGIIVGG
                     PGGGIDGRLNLFSNAGTAPNYCSVEYNMWFYPDETLEGVRAEVEELVSSVCRADPWLR
                     EHPPRFTWKLGHIYFPPLDVPLDHPAVVTLADCLTQVGLDAAPTGFEAATDLAWYAER
                     QLPGIICGPGSLAQCHAPDEDVDAEQLELAAKVYGLMVTEWCA"
     gene            complement(5295..>5739)
                     /locus_tag="AC150V_07800"
     CDS             complement(5295..>5739)
                     /locus_tag="AC150V_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR3762863.1"
                     /translation="LGAPPMNLIEGTLELVGGEWHFRSPHVDCVLPPEALNVSELEHE
                     AGSSVRFGLRPEHFQIGPLGQEGAIEGRVHFLEPVGSDLYLTVEIGEATLQVRTDPDS
                     PLRPGENVSLTFDPWRVHIFGADGHNLRRDSGPTESEPGLTQAAS"
BASE COUNT          878 a         1995 c         1967 g          899 t
ORIGIN      
        1 gcgcggctgc gcgccgccgg ggcgccgaac ccggacagcg tttacaaacg cctcgctgaa
       61 cgcgggccga ttgctctctt cgaggtctcg ccggtgccct tccggccgcg cctcgttttg
      121 acgctccgcc cgcgggcatc ctcccgctac acggacgcct tcaccacgga gctgtcgtgt
      181 ccgcggcagc gcgatctgga ctgcttccgc cgccgcgccg gcgagacagt cacggtgacc
      241 aagctcagct gagggcgcgc ctcatcccag cccttcgagg tatgcgacaa cctcgccgca
      301 cgtggttacc tccgcgtagc ggcggtggac gtcgcggagg ttcgactcgt gcgcgtcgcg
      361 cccctggtcg ccgacgcagt cctccggaac gagcgtgcgg aagccgtagg agaaggcgtc
      421 gaccgtcgtc gcgcggatgc agcccgacgt cgtgcagccg gtcacgacga ccgtgtcgca
      481 gcccgccgcg gtgagaagcg aaaccagcgg cgtgccgtgg aagaacgagg gccacttctt
      541 tgtgaggagc gtgtcgccgg cgtcccacac gcgctcgtcg acctccgccc agcgtgagcc
      601 tggggtgatc tcggcgcagg ccggcagctt gaccgcccac aggccgatgt cgcgctctcc
      661 ctcccaggcg acgaccgtct ggaacacggg cacggccgac cggcgcgctg ccgccgtgag
      721 gcgggcggtg ttcacggtgg cccgctcgat cagctcggcg ccgccgaacg ggaaggcggg
      781 atcggtgaac gcgtactgga ggtcgacgac gaggacggcg gggcgctcgc cggggctgat
      841 cgacgcctgg ccgtagccgc ggcgcaccca ggggtcgtcg gcggaaagct ccacgcgccg
      901 atcctctcag ttccgtcggc gttgccgctc agcacggccg gttggtcgca gaaggccgcg
      961 ctgcgcggcc tcctacgacg cgagccgaag tcctagccgg cgtgagcggc ttcgggtgtc
     1021 gggacggccc cgacctgctg cagcatgccg agcgcgtccc agttcgccca gctctcctgg
     1081 atcttgccgc ccgagatgcg gtcgatgaag atcccggaga gcgtgacctg cttgcccgtg
     1141 ggggcgatcc ccatcaggtc gccgttgtgc gtccccctca cggtccaccg ggaggcgacc
     1201 atgtcgcctt cggcgatctg gtcctcgatg gtcatcgtca tgtccgggaa cgcgccccga
     1261 tacatggaga tgaggtgctt gaggccttcc gcgcccctgg gcaggtccgg gggattggcc
     1321 ggatcgttgt cgatccagtc gggggcgacg atctcgtcga ccacctcgag gttccccttg
     1381 ctcaccgcct cgttgatcag gcgctgcatg agtttcttgt tgtccgcgga catcgagctc
     1441 cttccgtgtc gccgtctgat cgggcgggag catacgccca cgtggcggcc gttacaaggt
     1501 gggcgtgacg gctctgcagg tcagctgcga gggcgagtcg acgccgactc ggcgtcttcg
     1561 gcagcggcgg cgatcatcgc gtcgggcgta tgcccgagct cgtcgagagt cgcctgctgc
     1621 gcggtgaacg gctgcatggc ctcgggccag atcgggtctt cgacccatgg ggcgggagga
     1681 tcagggggca ggacggcgag cgtcctgccg ccgacgatcg tgcgggtgct ctcgagcagc
     1741 acgcggctct gccgcatcgt gccgtggatg tcgtagaccg ggaaacggcc ggagtggagg
     1801 cggaacagtg ccacgtcggc tcgcgagccg ggtcgcagtg tgccggcctc ctgctcgatt
     1861 ccgaggatct cggcgggcct cgaggtcacc gcctggacga catcgcgtag cgacatgcca
     1921 aggtgaagga acttgctcat gcacgtcggc atgtcgtagg ccggcccctt cgcgctcagc
     1981 tggtgcaggt cggtcgagat gacgtcgggt gcccgtccgg ctgccatcag cgcttccgcg
     2041 gtctcgaagg agaacgaccc ggtgccgtgg ccgatatcca tgatcacgcc ggcgtcccaa
     2101 gcgcgtgtcg cgaagtcgta gagctgaccg cgttcgtcga cgatcttcat cgtcaggccc
     2161 gtgaagcaat gcgtgaggat gtccccgggt cgcatcagcg cgagcacgtc ttcgatctcc
     2221 ggaggcccga tggcgatgtg caccatcagc ggcagctcgc attcctctgc ggcctgtcgg
     2281 gcccggcgca gcggctcgac gccgttctcg cccacggtcg gtgagccgat tcgcaccttg
     2341 actccgcgaa cgaagtctcg gttccggtca gccagcctgc ggaagaggcc gacgtcgagg
     2401 taggagaggt ttgcgcactc ctcgttgtcc gcgacgaggc cgatatcgga gatgttcagg
     2461 aaggccgtga gcgagacacg cgcggggtcg gcgacgaact cccgcaggcc ctgcagggtg
     2521 agcgcgcccg ccgaaccggc atcgttccag gtcgtgacgc ccgagcgcga cgcgaacgga
     2581 tcggggttga tcccccagta ggtcaccttg tggaagacgt gcgtgtggag atcgatcaac
     2641 ccgggcatga cgtacagccc ctcagcgtcg atcacgtcga acgcggcctc ggcagggatg
     2701 tcacgatcga cggcggccac ccgtcctcgg gtgatcgcga cgtcgagatt cccttcgttg
     2761 ccacccccgg ggtcgacgac ctcgcctccc cggatcagca ggtcgaatcg catctgcttt
     2821 cccttcctct tgtcacgtgg ctctccggca cggctacacc gccagcgcgg cgcgaatcca
     2881 atccaacgcc tccagacgaa cgagcccgta atggtagaag tcgacccgct cggcgcccag
     2941 gtcgcgggcc agccggaact tcgcagccag gttctccggt gactcgcagt cgggcggcga
     3001 cgggcgcagc gcgaccgcga ccgggcagtc gccgaggagc tctcggtacg aggagcaccg
     3061 tgtcgacgcc ctcgtcggcg aggtcggtcg cgaacgcgaa ggcgcaggag tgcgggctgc
     3121 ccgtaggagg tggagcggcc actcagccga cgaggtagcc gccgtcgacg acgatctgcg
     3181 ctccgttgat gtacgccgac agatccgaag cgagaaagac gacaacgcga gcgatctcgt
     3241 cgggatcggc ccacctacgc atgggaaccc gcttcgacgc ctcgtcccac tggcgctcga
     3301 ggtcgatccc ctccggggcg ccggccttga tgtactcgat cgtcccctcg gtcagcgacg
     3361 gcccgggcgc gatggcgttc gtccgaattc cgcgaggtcc cagctccaga gcgaggcact
     3421 tcgtcaagcc ccaaagggcg tgcttcgacg tcgtgtagtg cgccatcccc tgtgacgtcg
     3481 ggtgcagcgc gtcgatcgag gtcatgttga cgatggcgcc gccgtccccc tgctggatca
     3541 tctggcgggc cgcctgtctc gcacaccaga aagccccctg gacgttcacg tcgattgctc
     3601 cggcgaagtc gtccagtggc agctcgtgga tccgggtgtt cgagtagacg gcggcgttgt
     3661 tgacgagaac gtcgagccgg ccgaagcgct cgacggccgc cgagacaaga ccgtcgatct
     3721 cctccggaac cgacacgtcg ccgacggccc aggagacccc gtccccgtgg tgcgccagcc
     3781 gccccgccgc cgcctcgacc gcctcgggcc tgcgatcggt caggagaacc gctgctccgg
     3841 ccccggccag ccgtcgcgcg gcggcgaagc cgaaaccctg tgcggccccc gtcacgatcg
     3901 ccgcacgccc gtcgagcgcg agcagatcgt cgatcgacgt cgccgcgtag gtgtcgaagg
     3961 tcgacgccgc ccggtcgctc gtcacgcgca ccactccgtc accatcaggc cgtagacctt
     4021 cgcggcgagc tcgagctgtt cagcgtccac atcctcatcc ggagcgtggc actgtgcgag
     4081 actccccggc ccgcagatga tgccgggaag ctggcgctcc gcgtaccagg cgaggtcggt
     4141 cgctgcctcg aagcccgtcg gggcggcgtc caggccgacc tgtgtgaggc agtcggccag
     4201 cgtcacgacg gcgggatggt cgagcggcac atcgagcggc gggaaataga tgtgcccgag
     4261 cttccaggtg aaccgcggcg gatgctcgcg gagccacgga tcggctcggc agacggacga
     4321 gacgagctcc tcgacctccg ctcggacacc ctcgagcgtc tcgtccgggt agaaccacat
     4381 gttgtactcg accgagcaat agttcggcgc ggtgcccgca ttcgagaaca ggttgaggcg
     4441 gccgtcgatc cccccgccgg gcccgcccac gatgatcccc ggcgagagcg tgtcgtggcc
     4501 gggcggaagc atcgcgtgcg acttcgtatt cgcccactgc cgctcgagct cttgcagggc
     4561 gacgacgatc ttgatcgctt tctcgatcgc gttgactccc ccgccgccct tgccgccggc
     4621 gtgcaccgcg cgatagcgcg tcgacgcgtg cgcctcgatc ccctccacct cgatccgaaa
     4681 gtgcacgagg ccgccctcgg ccggacagag ctgcagctcg gtcggctcga gcgacagcgt
     4741 cacgtcggcg gcgggagccc gctcgacgat ctcgcgcgtg ccgttgccta cgacctcctc
     4801 gtcggtgacg acatggaaga agatgtcacc gctcggacgg aatccctgat cgagggcgac
     4861 cttgaccgcc cacatcgcgg cggccacgcc ccccttcatg tcggttgcgc cgcgaccgta
     4921 gatgcggccg tccacgagcg cgccgccgaa gggatcctcg gaccacgcac tcgtgtcgcc
     4981 gaccggcacc acgtccaggt ggccgttgaa ggcgagtgag cgcccgccgc ccgagcccgg
     5041 gagccgggcg atgacggccg gatgcgcgtc gaaggtggcc gacggcccga catcccagcg
     5101 ctccgactcg aagccgaggt cgctcaggaa cccgcatacg tagtcgacgc agccgcgaac
     5161 ctcttcgggg aagtgggcgg cctcctttgc ctggctctcg gtgcgaaagg cgatcagatc
     5221 gcgcagcaga tcgcgcagtc cctcgcgcga ggcggccaca gcctcagcga ccgcgggccg
     5281 ttgctccatc cgcgctacga ggccgcctgg gtcagccccg gttcggactc ggtcggcccc
     5341 gagtcgcgac gcaggttgtg accgtccgcg ccgaaaatgt ggacgcgcca cgggtcgaac
     5401 gtgagcgaga cgttctcgcc cgggcgcaga ggcgagtccg gatcggtccg tacctgcagc
     5461 gtggcctcgc cgatctcgac cgtcaggtag agatccgacc cgacgggctc gaggaagtgc
     5521 acccggcctt cgatggcgcc ttcttgacct aacggcccga tctggaagtg ctcaggccga
     5581 aggccgaatc gaaccgaaga ccccgcctcg tgctcgagct cggagacgtt cagtgcctcc
     5641 ggcgggagca cgcaatcgac atgggggcta cggaagtgcc attcgccgcc gaccaactcg
     5701 agcgtgccct cgatcaggtt catgggcgga gcgccgagg
//
LOCUS       DBNGNK010000265         4140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_690874,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000265 DBNGNK010000000
VERSION     DBNGNK010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_690874"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC150V_07805"
     CDS             <1..594
                     /locus_tag="AC150V_07805"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR3762864.1"
                     /translation="YAGAAAVCPELRPQLVGWERADSLIVNPHKWLATPMDCSALWTS
                     RPQAFRDTFSLVPDYLRSPDDAVNLSEVSVPLGRRFRALKLWAVLRCFGRAGLQERIR
                     EHVRLAALFESWVRDEPGWEVVAPRTFSLVCFRRDDTDEANEQLLARVNRSGEVFLSH
                     TTLEGRFVLRLAIGNYRTTEADVHLAWDVLRREAAVD"
     gene            640..2082
                     /locus_tag="AC150V_07810"
     CDS             640..2082
                     /locus_tag="AC150V_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR3762865.1"
                     /translation="MTQTDRTKWLGLGLICLVQFMVVLDIAIVNVALPSIQVDLNFAQ
                     DDLQWVISAYALAFGGFLLLGGRLGDLIGRRRVFVIGLAVFTVGSLLCGFAWSDVSLI
                     LFRALQGFGAALVSPAALAILTATFVEGRERNIALGVWGAVGGFGAAAGVLLGGILTD
                     LLAWEWIFWVNVPVGVVGIALAPLLLQESRDEAQHTFDLLGALFVTSHLLLLVLGITQ
                     GREWGWSSGRTIGVFVASAVFALGFLAWERRQRDPLVPFSIFRLRTVSAANAEGFILG
                     TVLFSMFLLLTLYMQQVLGYSALKTGVAYLAVAGTSVLWANVAAMTVNRFGVKPALIL
                     GMALLTGGLVYFTQISVDGSYWADLFPGFLIIGVGIPFGFVPITIAAVAGTGHDRAGL
                     ASGLINTSQQIGGALGIALLSTVAISYTDDLIAGGTAQPLAAVEGYQRAMWVGAAFAF
                     VGVLVAIFAVRKEDLPEQAEPSLEPASEVA"
     gene            complement(2083..2799)
                     /gene="otsB"
                     /locus_tag="AC150V_07815"
     CDS             complement(2083..2799)
                     /gene="otsB"
                     /locus_tag="AC150V_07815"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR3762866.1"
                     /translation="MCFESLRLLTTEPGRAAILLDVDGTLAPIVDDPRAASVPEETRA
                     ELRRLAGRYALVACVSGRPGEDAARVVGVRELIYVGEHGLELDPAAADWAERLHAFAS
                     TIAWPDLERKPLTVSFHYRRSPDREAARHELEHVAERAAAAGLVPRFGRMVLELRPPV
                     QASKATAVRHLLADHGLQRALYAGDDTTDIDAFEAMRELEEGVAVAVVTPESPPGLAE
                     AADVTVTGGTEELLDLLRSL"
     gene            2822..3169
                     /locus_tag="AC150V_07820"
     CDS             2822..3169
                     /locus_tag="AC150V_07820"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3762867.1"
                     /translation="MLRLLLIGLVAGVFSALFGVGGGILIVPLLIVVAAFPPREATAT
                     SLGAIGITALAGAGFYAVEGEIDVRYAALVGVPALFGVVFGASAQQRLSGRALGLAFA
                     VLIAGIGVWFLAT"
     gene            3166..3540
                     /locus_tag="AC150V_07825"
     CDS             3166..3540
                     /locus_tag="AC150V_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR3762868.1"
                     /translation="MTTTTILLALCLGGAAGVLAGLFGVGGGILFVPTLVWLGLGQVE
                     AAATSLLAIVPTAAVGIWRQSRYGNVDLRNSAIVGLASVVGVGAGVQLATSMPENVLR
                     KLFGVLLLGVAAQLAWRWRRAD"
     gene            3575..>4140
                     /locus_tag="AC150V_07830"
     CDS             3575..>4140
                     /locus_tag="AC150V_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762869.1"
                     /translation="MKSAYTLVPLGLAVLVTMVAGAAAQSESPTTATALVASVTVPGG
                     GGGGAERLVAPPAAELGSTSWAYPDDGSIVTIVLATGSAVAQHGASSSAQSVLDLNSI
                     VLFGGDVVVRALSIRAAAAAGSANTTADFSSSSVTGLTALGQSYPQASSQQIPLADWG
                     TLDILPGSVTSLTKPRPSATGSVTGLRIT"
BASE COUNT          518 a         1453 c         1411 g          758 t
ORIGIN      
        1 tatgcaggcg ccgctgccgt ctgcccggaa ttgcgcccac agctcgtcgg ctgggagcga
       61 gcagactcct tgatcgtcaa tccacacaag tggctggcga cgccgatgga ctgctccgcc
      121 ctctggacgt cgcgtccgca ggcgttccgc gacacgttca gcctggtgcc ggactacctc
      181 cgctctcccg acgacgcggt caacctgagc gaagtctccg tcccgctcgg ccggcgcttt
      241 cgcgccctga agctctgggc ggtgcttcgc tgcttcgggc gggccgggtt gcaggagcga
      301 atccgtgagc acgtacgcct ggcggcgctg ttcgagagct gggtgcgcga cgagcccggc
      361 tgggaggtcg tcgcaccgcg caccttctcg ctcgtctgct tccggcgcga cgacacggac
      421 gaggcgaacg agcagctgct tgcacgcgtc aaccgcagcg gcgaggtctt tctgtcacac
      481 acgaccctcg aggggcggtt cgtcctgcgc ctcgcgatcg gcaactatcg cacgaccgag
      541 gccgacgtcc acctggcctg ggacgtcctg cgccgagagg ccgccgtcga ctaggctgac
      601 gggctcccga ccgatcccga ccggaacgcc atctcggaga tgacacagac tgaccgcacg
      661 aagtggctcg gcctcgggct gatctgcctc gtgcagttca tggtcgtgct cgacatcgcg
      721 atcgtgaacg tcgcgctgcc gtcgatccag gtcgacctca acttcgccca ggacgacctc
      781 cagtgggtga tcagcgccta cgctctcgcc ttcggcggct tcctcctgct cggcgggcgg
      841 ctcggcgacc tgatcggtcg gcgccgcgtg ttcgtcatcg gcctcgccgt cttcacagtc
      901 ggttctctgc tctgcggctt cgcctggagc gatgtctcgc tgatcctctt ccgtgcgctc
      961 cagggcttcg gcgctgcgct ggtctcgccg gccgcgctcg cgattctcac agcgaccttc
     1021 gtcgagggcc gggaacgaaa catcgcgctc ggcgtctggg gagccgtcgg cggcttcggc
     1081 gcggcggccg gtgtcctgct cggcgggatt ctcaccgacc tgctcgcgtg ggaatggatc
     1141 ttctgggtca acgtgcccgt cggcgtcgtc gggatcgcac tcgcgccgct gctactccag
     1201 gagagccggg acgaggcgca gcacacgttc gacctgctcg gcgccctctt cgtcacgagc
     1261 catctcctgt tgctcgtact cggtatcacc cagggccggg agtggggctg gtcgtccggg
     1321 aggacgatcg gcgtcttcgt cgcctcggcg gtgttcgcgc tcggcttcct cgcctgggaa
     1381 cggcggcagc gtgacccgct cgtccccttc tcgatcttca ggttgaggac ggtttccgcc
     1441 gcgaacgccg aagggttcat tctcggcacg gttctgttct cgatgttcct gctcttgacc
     1501 ctctacatgc agcaggtgct cggctactcg gcgctgaaga ccggagtcgc gtatctcgct
     1561 gtggcgggga cgtcggttct gtgggcgaac gtggccgcga tgaccgtgaa ccggttcggg
     1621 gtcaagcccg ccctgatcct gggcatggcc ctactcacgg gcgggctcgt gtacttcacg
     1681 cagatctccg tcgacggctc gtactgggcc gatctctttc ccggattctt gatcatcggt
     1741 gtcggcattc cgttcgggtt cgtgccgatc accatcgcag ccgtggcggg caccggccat
     1801 gaccgagcgg ggcttgcgtc ggggctcatc aacacgtcgc agcagatcgg cggagcgctg
     1861 gggatcgcgc tcctttccac cgtcgcgatc tcgtacaccg acgacctgat cgccggcggt
     1921 acggcgcagc cgctcgccgc cgtcgagggc taccagcgtg ccatgtgggt gggcgctgcc
     1981 ttcgccttcg tcggcgtgct cgtcgcaatc ttcgccgtgc gcaaggagga tctccccgag
     2041 caggccgagc cctcgcttga gccggcgtcc gaggtcgcgt aactagagcg accgcagcag
     2101 gtcgaggagc tcttcggttc cccccgtgac cgtgacgtcc gcggcctcgg cgaggccagg
     2161 cgggctctcg ggtgtcacga ccgcgacggc gacgccctcc tccagctccc gcatcgcctc
     2221 gaacgcgtcg atgtcggtcg tgtcgtctcc ggcatagagc gcccgttgca ggccgtggtc
     2281 cgcgaggagg tgccggacgg ccgtcgcctt gctcgcctgc acgggcggcc ggagctctag
     2341 aaccatgcgc ccgaagcgcg gcacgagccc ggccgcggcc gcccgctccg cgacgtgctc
     2401 cagctcgtgt cgcgccgctt ctcgatcggg cgagcgccgg tagtggaagc tgaccgtcag
     2461 cggctttcgc tccagatccg gccacgcaat cgtcgacgcg aacgcatgga gccgctccgc
     2521 ccagtccgcg gcggcggggt cgagctcgag cccgtgctcg cccacgtaga tcagctcgcg
     2581 cacgccgaca acgcgcgcgg cgtcctcgcc cggccgaccg ctcacgcagg caacgagtgc
     2641 gtagcgaccg gccagccggc gcagttccgc tcgggtctct tcgggtaccg atgcagctcg
     2701 agggtcgtcg acgatcggcg ccagcgtgcc gtcgacgtcc agcaagatcg cggcgcggcc
     2761 cggttcggtg gtgagcaggc ggagagattc gaagcacacc gtgcaaccgt acgataggcg
     2821 cgtgctccgg ctccttctga tcggtctcgt cgcaggcgtc ttcagcgcgc tcttcggagt
     2881 cggcgggggc atccttatcg tgccgctcct gatcgtggtc gcggccttcc ctccgcggga
     2941 ggcgacggcg acctcgctcg gggcgatcgg gatcaccgcc ctcgcgggcg ccggcttcta
     3001 tgccgtcgaa ggcgagatcg acgttcggta cgcggctctc gtcggtgtgc ccgcgttgtt
     3061 cggcgtcgtc ttcggcgcct cggcgcagca acggctctcg ggccgggctc tcggcctcgc
     3121 cttcgcagtg ctgatcgccg gcatcggcgt ctggttcctc gcgacatgac gacgactacc
     3181 atcctgctgg ccctctgcct gggcggcgcg gccggggtgc tcgccggctt gttcggcgtc
     3241 ggcggcggaa tcctgttcgt gccaacgctc gtctggctgg gactcgggca ggtcgaggcg
     3301 gccgccacct cgctgctcgc gatcgtgccg accgctgccg tcggaatctg gcgccagtcg
     3361 cgctacggga acgtcgatct gcgcaactct gcgatcgtgg gtctcgcttc ggtcgtcggt
     3421 gtcggcgcgg gcgtgcagct cgcgacgtcg atgccggaga acgtcttgcg gaaactgttc
     3481 ggcgtccttc tgctgggcgt ggcagcccag ctggcctggc gctggcgtcg cgccgactga
     3541 tcttccgaga gatccatcgt ctaccctggc cggggtgaaa tcggcgtaca cactcgtccc
     3601 gctgggcctt gccgtgctcg tgacgatggt cgccggcgcc gctgcccagt ccgaaagccc
     3661 cacgactgcc acggcgctcg tcgcgagcgt gaccgtcccc ggcggcggcg gcgggggcgc
     3721 ggaacggctc gtcgccccac ctgccgcgga gctcggctcg acatcctggg cgtacccgga
     3781 tgacggctcg atcgtgacca tcgtgctggc gacggggagc gccgtcgcgc agcacggagc
     3841 ctccagtagc gcccagtcgg ttctcgatct caactcgatc gtgctgttcg gcggcgacgt
     3901 cgtcgtgcga gcattgagca tccgcgccgc ggcggcagca gggtcggcca acacaacagc
     3961 cgatttctcg tcgtcgtcgg taaccgggtt gaccgcgttg ggccagtcgt acccgcaggc
     4021 gtcgagccag cagatcccgc tggccgactg ggggacgttg gacatcctgc ctggttccgt
     4081 gacgtccttg accaagccgc ggccgagcgc aactggctcg gtgacgggtc tccggatcac
//
LOCUS       DBNGNK010000266         6519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1624541,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000266 DBNGNK010000000
VERSION     DBNGNK010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1624541"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="AC150V_07835"
     CDS             <1..1098
                     /locus_tag="AC150V_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762870.1"
                     /translation="DGTNWTLNTHESLDEVGWQIPQAVTVGPMGFTMVGTENLTIKPN
                     AELSGDFNAAVWHSEDGLSWERVPRDADVFGGVGAQNMLDVVARSAGLIAVGIDSQPD
                     PDSGLTVGAVWTSPDGARWTRVAGEPQLFRTIVDEPNRFTWIQAAAVDGEGRVVAVGS
                     DTHTPQPGTGIHSSSLAAVWFSADGESWERVAHEDAAFGDGTTVIDLFDVTAGGPGFV
                     AIGLEQQPLKNFEGGFPQVILRNEGHAGARGVIVTSEDGLVWNRVPNDQLPDAGEGAL
                     LWSIRTTNLGLVTVGYRRDNPGTADAEVDPIVWISTDGTTWTEAADPDGVFDQPGGQA
                     LLDVADGGPGLVAIGLDGGLKSSNTAIWTSP"
     gene            complement(1630..3198)
                     /gene="serA"
                     /locus_tag="AC150V_07840"
     CDS             complement(1630..3198)
                     /gene="serA"
                     /locus_tag="AC150V_07840"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR3762871.1"
                     /translation="MRVLVREPISSAGLELLREQFDVDVDGESELADIIGDYDAIIVR
                     SSTKLTGELLDRARSLKVIGRAGVGIDNVDVDAATRRGIVVANAPDSTVVSAAEHTVG
                     LLVALARNIPQAHAALKNGRWERSRWSGVELADKTLGVLGLGRIGQQVARRALGLGMR
                     VVACDPFVGEERFRELGVEAAESVDDLLGAAEFLTLHAPLTPETSRLIDRDAIAKMPD
                     GARIVNAARGGLIDEEALFEAIRSGKLAGAALDVFSSEPYSGPLLELDEVVVTPHLAA
                     STSEAQVRAGVVVAEQVAAALGGGLVMNAVNIPAVDLADLEVLGPFVPLAAKLGRLAV
                     ELAGGRSNRIVVAAHGPLADHDARLLTMAALNGVFQGRVDHAVNVVNASLIAAERGIE
                     VVEERFRASRDYTNLVEVRAATDGEDVTVSGTTIGPEGRLFLAGALGFSIDIELAPHM
                     VFVRYEDQPGVIGRIGTMFGQARVNIANMAVSRTKEGDRALMVFSIDTEASEALADEL
                     LEAGFLDARFIRLD"
     gene            complement(3195..4349)
                     /locus_tag="AC150V_07845"
     CDS             complement(3195..4349)
                     /locus_tag="AC150V_07845"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR3762872.1"
                     /translation="MATKRRLLTPGPTPVPPEVLSALAEPVIHHRSPDFKAVMKRVLD
                     RLREVFRTENDVLVYTASGTGAFESAIANVLSPGDTVLAVSAGNFGERWQKMASAYGC
                     DVRTLSYDWGRSPDADDLRRKLAETGAGVVFLVHSETSTGVVCDLEGLVAVCNDSGAL
                     SVVDAISSLGAVPLDTDAWGVDVVVTGSQKALMTPPGLAFVSVSDRAWARAEAGAMPR
                     FYWDWGRARKAQESFSSPFTPATSIVVALDEALRLVLEEGLEQAYARHVALGRAARSG
                     AKAMGLELYSPDDDSSAVLTALLTPAGADAVELRLALRDRHGITVAGGHGDIADRLFR
                     IGHIGYVDVFDITTALAAVEVELHRMGADVERGVGVAAALEAYEAAVPQP"
     gene            4474..5088
                     /locus_tag="AC150V_07850"
     CDS             4474..5088
                     /locus_tag="AC150V_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762873.1"
                     /translation="MNTLLAFAATLVSLRLTAELARRYRSRRSPELAAWAASLGAFAL
                     ASAALAWGAAAGWDEASFRVYYLAGALLTAALLGTGSLLRIGRSWITPVALLYAGLAV
                     GVAISAPLTEPVAGTEIPAAQDHLELWPARVLAIAANSLGTLAAVGIAVWTLRARPVG
                     NTLLVAGIAVAALGSALAGLGEAGSALFAAAASVLLYGGFVSSR"
     gene            complement(5085..5270)
                     /locus_tag="AC150V_07855"
     CDS             complement(5085..5270)
                     /locus_tag="AC150V_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762874.1"
                     /translation="MLVGHGGVYGAFGEAALALALAGLFVWIWLRERRKSRERGDEPA
                     EMNDDAPSWRDESDVPR"
     gene            complement(5278..>6519)
                     /locus_tag="AC150V_07860"
     CDS             complement(5278..>6519)
                     /locus_tag="AC150V_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HIR3762875.1"
                     /translation="LWKQPLLARRTGRALPTGVSRLVLGPLRVALQVVSALLFLLVFV
                     SALVGDTDPFDNLAPTWIYVIFWLGVPLLSVLFGNVWRALSPWRALADGFVWAWELTG
                     REARPLAAYRQRWGRWPGVVALFAFVALELAYYDPSDPRALALAIAVYTYVTLFGFVM
                     FGREAWTDGGEGFAIAFAYLARVAPLAAKDDRIVARWPFTGLVGAEPVAGSLAFIAVM
                     LGSVAFDGFSRASVWQDLLADVQAPYVLEHPTIADLFGSGVNLLGLAAFCLLVAGAYR
                     LACATARSSVNAPRSLVPDFLSSLIPIAFVYLVAHYFTLFVIQGQFTIPLLSDPLGRG
                     WDAIGTAGYKPNLTPFSTNTIWYVQVAALVIGHVAGLAVAHDRAITIFRDRGAALRSQ
                     YAMLALMVLYTVGGLWLLSRG"
BASE COUNT         1103 a         2271 c         2203 g          942 t
ORIGIN      
        1 gacgggacga actggacgct caacacccac gaaagcctcg acgaggtcgg ctggcagatc
       61 ccgcaagcag tgaccgtagg acccatgggc ttcacgatgg tcggcaccga gaacctcacc
      121 atcaagccca atgccgagct ctccggcgat ttcaacgcgg cggtctggca ctcagaagac
      181 gggctgagct gggagcgtgt tccgcgcgac gcagacgtgt tcggcggcgt tggcgcccag
      241 aacatgctcg atgtcgtggc acgtagcgct ggcctgatcg ccgttgggat cgattcgcag
      301 ccagaccccg attccggtct cacggtgggg gcggtttgga cgtcgcccga cggcgcacgg
      361 tggactcggg tggcaggcga gccgcagctt ttcaggacga tcgtggacga gccgaaccgc
      421 ttcacctgga tacaggccgc cgcggtcgat ggggaaggac gggtggtcgc ggtcggaagc
      481 gacacgcaca cccctcagcc ggggacgggt attcattcca gcagcctcgc cgcggtctgg
      541 ttctcggccg acggcgaatc ctgggagcgt gtcgctcacg aggacgcagc gttcggcgac
      601 ggaacaactg tcatcgacct gttcgacgtc accgcgggcg gtcccggctt cgtcgcgatc
      661 ggcctcgaac agcagccgct gaagaacttc gagggtggtt ttccccaggt catcctccgg
      721 aacgaagggc atgcgggggc gcgcggagtc atcgtgacct ccgaggacgg cctcgtctgg
      781 aaccgcgtcc caaacgacca actccccgac gcgggagaag gtgccctgct ctggtcgatc
      841 cgcaccacga acctgggact cgtcaccgtc ggctaccggc gcgacaaccc cggcaccgca
      901 gacgccgagg tcgacccgat cgtctggatc tcaaccgacg gcactacctg gaccgaggca
      961 gccgaccccg acggggtgtt cgaccagccc ggcggacaag ccctcctcga cgtcgcggac
     1021 ggcggaccag gcctcgtcgc gatcggtctc gacggcggcc tgaagagctc caacaccgcc
     1081 atctggacat cgccctagtc ggcgcgtttg caatgtttgc agtccgggcc cagcccgcat
     1141 gagctctcaa ccgttgtaaa tagaaaaggc gcccgtattt gcaggccttc ttcggaagcc
     1201 gacgcccgga ctcgaaccgg ggacctccat cattaagagt gaaaggggtg gcggcgccgg
     1261 tgcgagccga cgctcaagcg cagggagcgt gcttcctcgc gtccccgcta gccgtccgcg
     1321 agcgccagcc gccacccctc cgcacgatcc gacgcgacgt cgccgcactc gacgcaccgc
     1381 agtagccggc ggtcgaggtg cctcctacgg cggctggtaa agccgcgaca cgaaggagcg
     1441 aacggttttc ccgacagcgg gtggcgcagc tcgtcgccga cgactgacgt tgccagccct
     1501 gcagacgatc ccgaggtcac accggggtca caccagcagc gcatttcacg acgtatacag
     1561 cctgggcaac cgcttgaaat atccgaatcg cttgtctgtg cggctcctgt tattccccgc
     1621 gcaaacccct caatcgaggc ggatgaagcg cgcgtcgagg aagccggcct cgagcaactc
     1681 gtctgcgagg gcctccgacg cctccgtatc gatcgagaac accatgagcg cccgatcgcc
     1741 ctctttcgtc cgtgagacgg ccatgttcgc gatgttcacg cgcgcctggc cgaacatcgt
     1801 cccgatgcgc ccgatcacgc cgggttgatc ttcgtagcgc acgaagacca tgtgcggcgc
     1861 gagctcgatg tcgatgctga agccgagcgc cccggcgaga aagagccttc cttccgggcc
     1921 gatcgtcgtg cccgagacgg tgacgtcctc gccgtcggtc gcagcgcgaa cctcgacgag
     1981 gttcgtgtag tcgcgggacg cgcggaatcg ctcctccacc acctcgatcc cccgctctgc
     2041 cgcgatcagc gatgcgttca cgacgttcac ggcgtggtcg acccggccct ggaagacgcc
     2101 gttgagcgca gccatcgtga gcaggcgcgc atcgtggtcg gcgagcgggc cgtgcgccgc
     2161 gacgacgatg cggttcgagc gtccccccgc aagctccacc gcaaggcgac cgagcttggc
     2221 ggccagcggc acgaagggcc cgagcacctc gaggtcggcg aggtcgaccg ccgggatgtt
     2281 gacggcgttc atgacgagac cgcccccgag agccgccgcg acctgttcgg ccaccacgac
     2341 tccagctcgg acttgggcct cgctcgtgga cgctgccaga tgaggagtga ccacgacctc
     2401 gtcgagctcg agcagcgggc cgctgtaggg ctcgctcgag aacacgtcca gggcggcacc
     2461 ggcgagcttg ccgctgcgaa tcgcctcgaa aagggcttct tcgtcgatca ggccgccgcg
     2521 ggcggcgttg acgatacggg ctccgtccgg catcttggcg atcgcgtcac ggtcgatcag
     2581 ccggctcgtc tcgggcgtga gcggcgcatg gagcgtgagg aactccgcgg caccgagcaa
     2641 atcgtcgacc gactcggccg cctccacgcc cagctctcga aaccgctctt cgccgacgaa
     2701 cggatcgcag gccacgaccc gcatgccgag cccgagcgcg cgccgcgcca cctgctggcc
     2761 gatgcgcccc aatccgagca cgccgagggt cttgtcagcc agctcgacgc cgctccagcg
     2821 cgagcgctcc cagcgcccgt tcttcagtgc ggcatgcgcc tgagggatgt tccgagcgag
     2881 cgccaccagc agcccgacgg tgtgctcggc cgcggagacg acggtcgagt cgggcgcgtt
     2941 tgccacgacg atccctctgc gggtcgccgc gtccacgtcc acattgtcga taccgacgcc
     3001 ggcccgaccg atcaccttca gtgaacgggc tcggtcgagg agctctccgg tgagcttggt
     3061 cgacgagcgg acgatgatcg cgtcgtagtc tccgatgatg tccgcgagct cggattcgcc
     3121 gtccacgtcc acgtcgaact gctcgcgcag cagctccagg cccgctgacg agatcggctc
     3181 acggacgaga accctcacgg ctgcggcacc gcggcttcgt acgcctcgag cgctgcggcc
     3241 acgccgacgc cgcgctcgac gtcggcaccc atgcggtgca gctcgacctc gaccgcggcc
     3301 agcgccgtcg tgatgtcgaa gacgtcgacg tatccgatgt ggccgatgcg gaagagccgg
     3361 tcggcgatgt cgccgtgccc gccggcgacc gtgatcccgt gtcggtcgcg cagagcgagc
     3421 cgcagctcga ccgcatccgc gcccgccggc gtcaggagag ccgtgagaac cgccgagctg
     3481 tcgtcgtccg gggagtacag ctcgagtccc attgccttcg cgcccgaccg ggcagcgcga
     3541 cccagcgcaa cgtggcgtgc gtacgcctgc tcgaggcctt cctccagtac caggcgcagg
     3601 gcttcgtcga gcgcgacgac gatcgaggtc gccggcgtga agggagagct gaaactctcc
     3661 tgcgccttcc gagcccgccc ccaatcccag tagaaacgcg gcatggcgcc ggcctccgcg
     3721 cgtgcccagg cgcggtccga gacggagacg aaggcgaggc ccggcggtgt cataagcgct
     3781 ttctgcgatc cggtgacgac gacatccact ccccacgcgt ccgtatccag cggcaccgcg
     3841 ccgaggctcg agatcgcgtc gacgacggag agggcgccgg agtcgttgca gactgcgacg
     3901 aggccttcca gatcgcagac gacgccggtc gatgtctcgg agtggacgag gaagaccacg
     3961 ccggcgcccg tctcggcgag cttccggcga agatcatcgg cgtccggcga gcggccccag
     4021 tcgtagctca gggtgcggac gtcgcagccg taggcgctgg ccatcttctg ccagcgctcc
     4081 ccgaagttcc cggcggaaac ggcgagcacc gtatcgccgg gggaaagcac gttggcgatc
     4141 gcagactcga acgcaccggt gccggaggcc gtgtagacga gcacgtcgtt ctccgtcctg
     4201 aacacttctc gtagccgatc cagtacccgt ttcatcacgg ccttgaagtc gggcgagcgg
     4261 tggtggatca ccggttcggc cagcgccgac agcacctcgg gtggcaccgg agtcgggccc
     4321 ggagtgagca ggcgtcgttt cgtggccatg gctgacagag cgtaccgccg cgaccgccgg
     4381 gcccgactgg gcgtcagacc ggaggcagac cgtttcagcc ttgtctcttc cggcggaacc
     4441 cctcacgctg caggcggcac tacgctgccg cggatgaaca cgctcctcgc cttcgcggcg
     4501 acgctggtct cgttgcgtct gaccgccgag ctcgcacgcc gctaccggtc gcgccgatct
     4561 ccggagcttg ccgcgtgggc ggcctcgctg ggcgcgttcg cgcttgcgtc cgcggcgctc
     4621 gcctggggcg cggccgccgg ctgggacgaa gcttcgtttc gcgtttacta cctggccggg
     4681 gcgctgctaa cggcggctct gctcggaacg ggatcgctgc tgcggatcgg gcggagctgg
     4741 atcacgccgg tcgcattgct gtacgcggga cttgcggtcg gtgtcgcgat ctcagccccc
     4801 ctcaccgagc cggtcgcggg caccgagatc cccgcggcgc aggaccacct cgagctctgg
     4861 ccggcgcggg tattggccat cgcagcgaac tcgctcggca cgctcgccgc ggtgggcata
     4921 gctgtctgga cgctccgcgc ccgaccggtc gggaacactc tgcttgtggc aggcatcgcc
     4981 gtggcagcgc tcggaagcgc cctcgccgga ctgggcgagg ccggctccgc cctcttcgcg
     5041 gcggcggcct ccgtactcct ctacggcggc ttcgtctcgt cccgctagcg agggacgtcg
     5101 gactcgtcac gccaggaagg cgcgtcgtcg ttcatctcgg cgggctcgtc tccccgctct
     5161 cgcgacttcc gtcgttcgcg cagccagatc cagacgaaga gacccgcgag cgccagcgcg
     5221 agcgcggcct cgccgaaggc gccgtagacg ccgccgtgcc cgacgagcac taggcagtca
     5281 gccccgggag agcagccata gcccgcccac cgtatacagc accatcaacg cgagcatcgc
     5341 gtactgggag cgtagcgccg ctccacggtc gcgaaagatc gtgatcgcgc gatcgtgtgc
     5401 gaccgcgagc cctgcaacat gcccgatgac gagggcggca acttgcacgt accagatcgt
     5461 gttcgtcgag aatggcgtga ggttcggctt gtagccggcg gttccgattg cgtcccagcc
     5521 gcgcccgagc ggatccgaga gcaacgggat cgtgaactgg ccctggatga cgaacagcgt
     5581 gaagtagtgg gcgacgagat agacgaacgc gatggggatc aagctcgaca ggaagtcggg
     5641 cacgagtgat cgtggcgcgt tgacgctcga gcgcgcggtg gcgcatgcga gccggtaggc
     5701 accggcgacc aggaggcaga aggcggccaa cccgagcagg ttcacaccgc tgccgaagag
     5761 atcggcgatc gtcggatgct ccaggacgta gggcgcctgg acgtcggcga gcaggtcctg
     5821 ccagacgctc gcgcgcgaga agccgtcgaa cgccaccgag ccgagcatga cggcgatgaa
     5881 ggcgagcgag cccgcgaccg gctcggcccc cacgagcccg gtaaacggcc agcgtgccac
     5941 gatccggtcg tccttcgccg caagcggcgc cactcgcgcc aggtacgcga acgcgatcgc
     6001 gaagccctcg ccgccgtcgg tccacgcttc gcgcccgaac atcacgaaac cgaagagcgt
     6061 gacgtacgtg tagacggcga tcgcaagcgc gagagcgcgc ggatcggacg ggtcgtaata
     6121 cgccagctcc agcgccacga acgcgaagag cgcgacgacg cccggccagc gcccccagcg
     6181 ttgccggtac gcagccagcg ggcgtgcttc gcggccggtc agctcccatg cccagacgaa
     6241 cccgtccgca agcgcacgcc aggggctcag ggcgcgccag acgttgccga acaggaccga
     6301 cagcagcggc acgccgagcc agaagatgac gtagatccag gtaggcgcaa ggttgtcgaa
     6361 cggatcggtg tcaccgacca gcgccgacac gaagacgagc aggaagagca gcgcggagac
     6421 cacctgcaag gcgacccgaa gcggcccgag gacaaggcgt gagacacccg tcggaagcgc
     6481 ccggccggtg cgtcgggcca gcagcggctg cttccagag
//
LOCUS       DBNGNK010000267         4303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_907763,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000267 DBNGNK010000000
VERSION     DBNGNK010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_907763"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..548
                     /gene="folK"
                     /locus_tag="AC150V_07865"
     CDS             87..548
                     /gene="folK"
                     /locus_tag="AC150V_07865"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011036940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR3762876.1"
                     /translation="MRRAYVGLGANLGPREETLRRALEKLAAAPGVELRRVSEFRETD
                     PVGRADQPRFLNAAAAVDTAFGPRELLDLLLSIERELGRMRPGPRFGPRTVDLDLLVY
                     ADVVVDEPGLTVPHPRLHERRFALEPLADLEPALDVPGRGTVAALLQELDE"
     gene            589..960
                     /locus_tag="AC150V_07870"
     CDS             589..960
                     /locus_tag="AC150V_07870"
                     /inference="COORDINATES: protein motif:HMM:NF022079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HIR3762877.1"
                     /translation="MSHLDELDEFEADLELRLKREYTAVFSLFRYCILTQDATYLCNI
                     LDLKAVPQAAYPFFHLKMEDVWVWDKNRPTRIIPRAEVYTSGDVTVEELRPEGDETSL
                     TAEALAERLGEPLPSDDETAP"
     gene            complement(957..1214)
                     /locus_tag="AC150V_07875"
     CDS             complement(957..1214)
                     /locus_tag="AC150V_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762878.1"
                     /translation="MSPFFYRSRYMPCADCGGSVAESDRDDHVCEPERLLDYRLFQLR
                     GELDAFDDQLGAYLDTPAGRFEEWCAKRRRIGGADGDSRSF"
     gene            1379..2131
                     /locus_tag="AC150V_07880"
     CDS             1379..2131
                     /locus_tag="AC150V_07880"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR3762879.1"
                     /translation="MLLAVDVGNTQTVLGLYQEESGLREHWRVATEQTRTGDELGVLL
                     EGLLELDVVDGVCLACTVPTLIREWERVADRWAKAPILVVGPGTKTGIPIRYDDPREV
                     GPDRIVNSVAAKARYGAPVIVVDFGTSTNFDVVSPEGEYVGGVLAPGIETSMEALFER
                     AARLVKVDYVEPPSVIGKTTVGGLQSGIVYGFAGQVDGIVAAIRRELGADAPVVATGG
                     LADLVEPHSAAIERVDPFLTLDGLKLVWELNS"
     gene            complement(2274..3332)
                     /locus_tag="AC150V_07885"
     CDS             complement(2274..3332)
                     /locus_tag="AC150V_07885"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR3762880.1"
                     /translation="MNIDLRSSWSIGPVDVPTRLVLAPMAGVSVQAFRRQGRRFGAGL
                     VCSEMVSCAGIEHRNEKTLGYLRVASDEHPLAIQIFGSEPRAMAEAARMVEAAGADIV
                     DINYGCPVKKVTKTGAGATLLEDPTRACSIVESVAAAVSVPVTVKMRRGLADGSQACL
                     ELGPQLVGAGAASLTLHPRSARQMYTGTADHALTAELVSLVDVPVVASGDVTTRAKAQ
                     SVLATTGASAVMVGRGAQGNPWALRDIIEGEVAEPSREEVVAELVLFVRETVRELGER
                     RATGFLKKFYGWYLGRGRFPKPFKQELVELETVAEVEERLFAAAPGAASILARLEAEI
                     PSVDEVTLELPVSIYGGG"
     gene            3453..3926
                     /gene="greA"
                     /locus_tag="AC150V_07890"
     CDS             3453..3926
                     /gene="greA"
                     /locus_tag="AC150V_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012809648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR3762881.1"
                     /translation="MKDVILTPEGYESLKQELDVLRGEKRREVADRIRVAREFGDIAE
                     NAEYDDAKNEQAMLEHKIAQLEERLSHARVIDTGDVDTSVVSVGSVVRLRDLDAKQTV
                     EYHIVGSAEANPAERKLSNESPVGKAIMGRKKGETVEVAVPRGKLKFKIMAIKAA"
     gene            4123..>4303
                     /locus_tag="AC150V_07895"
     CDS             4123..>4303
                     /locus_tag="AC150V_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR3762882.1"
                     /translation="MSLPKRFPDRDDVATVRADCELLEAGQEADVTRRLAGRAMARRG
                     MGKLVFLDLVDRSGRI"
BASE COUNT          665 a         1503 c         1476 g          659 t
ORIGIN      
        1 gcgagtgcga gcacgcgacg gtagttgtac gcgacgcttg taatactgcg ctgagggatc
       61 ggggaaggcg cagttaaggt cacgttatgc ggcgggcgta cgtcggtttg ggtgccaacc
      121 tcggtccgcg ggaagagacg ctgcgccggg cactcgagaa gctcgccgcc gcccccggtg
      181 tggagctcag gcgcgtgtct gagttccgcg agacagaccc cgtcggtcgt gccgaccagc
      241 cgcggtttct gaacgcggcc gccgcggtcg acacggcgtt tggtccacgt gagctgctcg
      301 acctgctgct ctcgatcgaa cgcgagctcg gccggatgcg cccaggcccg cggttcggcc
      361 cgcgcacagt cgatctcgat ctgctcgtgt acgcggacgt cgtcgtcgac gagccgggac
      421 tgaccgtgcc gcacccgcgc ctgcacgagc gacggttcgc cctcgagccg ctggcggacc
      481 tcgagccggc cctcgatgtc ccgggccgag gaacggtggc agcgctcctc caggagctgg
      541 acgaatgagc gctcagcccc gcgccgccga ccatctacac tgcggttcat gtctcatctc
      601 gacgagctcg acgagttcga ggccgatctc gagctgcgcc tcaaacgcga gtacactgcc
      661 gtcttctccc tgttccggta ctgcatcctg acccaggacg cgacgtacct ctgcaacatc
      721 ctcgacctga aggcagttcc gcaggccgcg taccccttct tccacctcaa gatggaagac
      781 gtctgggtgt gggacaagaa ccgtccgacc aggatcatcc cgcgagccga ggtgtacacg
      841 tccggtgacg tcacggtcga ggagctccgt cccgagggcg acgagacgag cctgaccgcc
      901 gaagccctcg ccgagcggct cggcgagccc ttgccgtcgg acgacgagac ggcgccctag
      961 aacgacctgc tgtcgccgtc ggcgccgccg atgcgccggc gcttcgcaca ccactcctcg
     1021 aagcgcccgg ccggtgtgtc gagataggcg ccgagctggt catcgaacgc gtcgagctcg
     1081 ccgcggagct ggaacaagcg gtagtcgagc aagcgctccg gctcgcagac gtgatcgtca
     1141 cgatccgact ccgcgaccga gccgccgcag tccgcgcacg gcatgtacct cgaccgatag
     1201 aagaaagggg acatcgtcgc tgcccgtcct agggcaggta tcggccggat gccgcgccgc
     1261 tgaagacccg gcgcgtccca cacggtccgt tcctcgacga cctgtccgcg ccgccggccg
     1321 tgcgcggacg acgcggggct ctctcgcgcg gccaggcgcg ctctgcttca cttgccgcgt
     1381 gctcctagcg gtcgacgtgg gcaacacgca gacggtcctc ggcctgtatc aggaggagtc
     1441 gggtctgcgc gagcactggc gcgtcgcgac ggagcaaacg cgcaccggcg acgagctcgg
     1501 cgtgttgctc gaggggctgc tcgagctcga cgtcgtggac ggtgtctgtc ttgcgtgcac
     1561 ggtgcccacg ctgattcgag agtgggagcg cgtcgccgac cgctgggcga aggcgccgat
     1621 cctcgtggtc gggccgggaa cgaagacggg cattccgatc cgctacgacg atccccgcga
     1681 ggttggcccg gaccggatcg tcaactcggt ggctgcaaag gctcggtacg gagctccggt
     1741 gatcgtggtc gacttcggca cctcgacgaa cttcgacgtg gtctcgccgg agggcgagta
     1801 cgtcgggggg gtgttggctc ccgggatcga gacgtcgatg gaggcgctgt tcgaacgtgc
     1861 agcccggctc gtcaaggtcg attacgtcga gccgcccagc gtgatcggca agacgacggt
     1921 cggcggcctg cagtcgggga tcgtctacgg attcgcgggc caggtcgacg gcatcgtcgc
     1981 ggcgattcgc cgggagctcg gcgccgacgc accggtggtc gcaacgggcg ggctggccga
     2041 cctcgtcgag ccgcactcgg cggcgatcga gcgggtcgac cccttcctca cgctcgacgg
     2101 gctgaagctc gtttgggagc tcaactcgta gtgctcaccc acgagccctc cttcgccggt
     2161 gcgcagagaa catctgtccc cttgcctctc ggccggcaaa gcctgcctcc cgtcgtcacc
     2221 cgcgccgcac cgggctcgct aggacgtggg ggaaccagga ggttccccac ctctcagccg
     2281 ccgccgtaga tggagacggg gagctcgagc gtcacttcgt cgaccgacgg gatctccgcc
     2341 tcgaggcggg ccaggatcga ggcggcaccg ggtgcggccg cgaagagccg ctcctcgacc
     2401 tcggccaccg tctccagctc gaccagctcc tgcttgaacg gcttcgggaa ccggccgcgc
     2461 cccaggtacc agccgtagaa cttcttcagg aaccccgtcg cgcgccgctc gccgagctcc
     2521 cgcaccgtct cccgcacgaa gagaacgagc tcggccacca cctcctcgcg gctcggctcc
     2581 gcgacttcgc cctcgatgat gtcacgcagc gcccacggat tcccctgcgc gccgcggccg
     2641 accatcactg ccgacgcgcc ggttgtcgcg aggacgctct gcgccttcgc gcgagtcgtg
     2701 acgtcgccgc tcgcgactac gggcacgtcg acgagcgaga cgagctccgc ggtcagcgcg
     2761 tgatccgccg tccccgtgta catctggcgg gcgctgcggg ggtgcagcgt gagcgaggcg
     2821 gcgcccgccc ccacgagctg cggcccgagc tcgagacagg cctgcgagcc gtcggcgagg
     2881 ccccggcgca tcttcaccgt caccggcacc gagacagctg cggcgaccga ctcgacgatc
     2941 gagcaggcgc gtgtcgggtc ctcgagcaga gtcgcgcctg ccccggtctt cgtgactttc
     3001 ttcaccgggc agccgtagtt gatgtcgacg atgtctgcgc ccgccgcctc gaccatgcgc
     3061 gccgcctctg ccatggcacg cggttcggag ccgaagatct ggatcgcgag cgggtgctcg
     3121 tcggaagcga ctcggaggta gccgagcgtc ttctcgttcc ggtgctcgat cccggcgcag
     3181 ctgaccatct cggagcagac gagacccgct ccgaaccggc ggccctgacg tcggaatgcc
     3241 tgcacgctga cgccggccat cggggccagc acgaggcgtg tcggcacgtc gaccgggccg
     3301 atcgaccacg aggagcggag gtcaatgttc acccgctcaa tcgtagtcgg gaaggtgttg
     3361 accgccgtct gtcgtgacag ctaccattct cgactcgcgt ggcgcgtcga gcgccgcgtt
     3421 tcctttcgtg caccgcttgg aggccgtttc gtttgaagga cgtcatcctc actccggagg
     3481 gctacgagag cctcaaacag gagctcgatg tcctgcgcgg cgagaagcgc cgcgaggtgg
     3541 ctgatcgcat ccgtgtggcg cgggaattcg gcgacatcgc cgagaacgcc gagtatgacg
     3601 atgcgaagaa cgagcaggcc atgctcgagc acaagatcgc gcagctcgag gagcgcctct
     3661 cgcacgcgcg cgtgatcgac acgggtgacg tggacaccag cgtcgtctcc gtcggctccg
     3721 tcgtgcgcct tcgcgacctc gacgccaagc aaaccgtcga gtaccacatc gtcggatcgg
     3781 ccgaggcgaa tccggccgag cggaagctct cgaacgagtc gcccgtcggc aaggcgatca
     3841 tggggcgtaa gaagggcgag accgtcgagg tggccgtgcc tcggggcaag ctgaagttca
     3901 agatcatggc catcaaggcc gcctagcaac gggcagcccc gggcccgacg ccgccggccg
     3961 ccgagcggtc gctcggcttg cgcgctttcg ccccagccga aatcctgcag cgaccgacgt
     4021 cgagggtgat cccggagccg gccacgagca cggcctgaac gcgcgggcgg acgggcccgt
     4081 ccggcgcccg cccgcccgct ctccccacta gactcgcggc cggtgagcct gccgaagcga
     4141 tttcccgacc gggacgacgt ggccacggtg cgggccgact gcgagttgct cgaggcgggg
     4201 caggaggccg acgtcacgcg ccgcctcgcc gggcgggcga tggcgcggcg tggcatgggc
     4261 aagctcgtgt tcctagacct cgtcgaccgc tccggtcgga tcc
//
LOCUS       DBNGNK010000268         5211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1193424,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000268 DBNGNK010000000
VERSION     DBNGNK010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1193424"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC150V_07900"
     CDS             <1..190
                     /locus_tag="AC150V_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR3762883.1"
                     /translation="QVAMYLSRELTDSSLPKIGKEFGGRDHTTVIHATSKITRMINED
                     RTVYNLVQELTARIKQHR"
     gene            350..1471
                     /gene="dnaN"
                     /locus_tag="AC150V_07905"
     CDS             350..1471
                     /gene="dnaN"
                     /locus_tag="AC150V_07905"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012931491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR3762884.1"
                     /translation="MEVETTVETGLEIRCDRDELAARLGLVARAVSTRGTVQVLGGVL
                     IRAQEGSLELAGTDMELSLRTSLPGEVDGEGALVVPGRLLVDLVRLLPASEVTIAYRP
                     EDGAAHVTSGSYASRVNVYSADDFPRLPTVDIPLHAVDRESLVGTIERVSKAASRDES
                     RPVLTGILVRFEGQQLIMAATDSYRMAVKETPLAESGPDLDAIIPARALSELTRFAVG
                     DKVSLGVQENHVVFGVDDVWLTTRRIDGQFPNYKQLRPESFEVELDLPRGELLEVVRR
                     AAVLAQRNSPLRLRFAEGELSVSAQTQDVGETSESLPAAYTGEPLEIGFNADFLREGL
                     ESVSGDEVRLSLINPLRPGLITSEGDPFWYLIMPIRLAG"
     gene            1471..2532
                     /gene="recF"
                     /locus_tag="AC150V_07910"
     CDS             1471..2532
                     /gene="recF"
                     /locus_tag="AC150V_07910"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR3762885.1"
                     /translation="MVRRTRLVNYRSYAELELALRPGLVLVVGENGAGKTNLLESLHV
                     GTQGFSPRTRHDAQLIRFGADQGAVSIDGERGGISTRTEIVLSLQEPKRATLNGARLR
                     AAEQLRSEVSTLVFTPDRLTVVKGGPAARRAYFDRTLGRLLPGRVSLPAEYASALVQR
                     NAALRRIGAGLSTREALAPWTERVAGLGADLVASRAEALAVLSPRFAEQAEMLGLAAA
                     ELSYEGEPPSTAVLEARLERDLDRGTTGAGPHLDDVAVLAASRDLRRFGSQGEQRIAV
                     LSLLLAEAALVGERQGVPPLLLLDDVLSELDARRRAALGERIRGLGQTVVTATSRDAM
                     PVVPDQVLDVTPGQVRQAA"
     gene            2532..2999
                     /locus_tag="AC150V_07915"
     CDS             2532..2999
                     /locus_tag="AC150V_07915"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR3762886.1"
                     /translation="MRRLGDDARRVLREAGMPEAGELAEITRAWPAAVGDAISRSAWP
                     SRVGRDGTLHVATVSATWAFELDRLSGELAERLTEALGARAPRALRFAPGNVPAPAAE
                     PTGRASRARPEPGERERREGASLAEEISDDELRKLVARAASASLAKGRDDHRF"
     gene            3047..>5211
                     /locus_tag="AC150V_07920"
     CDS             3047..>5211
                     /locus_tag="AC150V_07920"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR3762887.1"
                     /translation="MTDTSYTAKDITVLEGLEPVRLRPGMYIGSTGPRGLHHLVYEVV
                     DNAVDEALAGRNDLVDVTIHPDNSVTVRDAGAGIPVDVIEDQGLSALTVVLTKLHAGG
                     KFGGEGYKVSGGLHGVGVSVVNALSEWLVAEVRRDGRLYRQEFARGAPTGEMADGGKA
                     DGSGTTISFLPDREVFETIELSATTLVQRLRETAFLTRGLRIVLRDERAGGKTEEFHY
                     EGGIRDFVAYVNDTKDAVHKNVIFLEGESDDGQVEVAMQWNSSYVESVFSFANNINTT
                     EGGTHLSGFRAALTGTLNRYARDKGLLKEKEDNLEGEDVREGLAAVISVKLRSPQFEG
                     QTKTKLGNPPIEGLVKTTVNQRLAEFLEENPTQARQIITKAISAARARQAARKARELT
                     RRKSALENSALPGKLADCTIKDADSAELFIVEGDSAGGSAKQARDRTYQAILPLRGKI
                     INSEKNRINKVLSNTEIQAMITAIGTGFGDEFDIANIRYGRIIVMTDADVDGAHIRTL
                     VLTFLYRTMPELFERGHVYIALPPLYHVKLGNRGMYFEKDAQLEELLVRERVTEMTVA
                     DRDGGEQSLSEARWARFVRDLAQFEAWSAKLESEFGAAARFAVEHRLIETEGDESTDV
                     ESLIDAVPPNGYELTVDERLEDRIKVKVVDAETNAARFVEIPGAMFSAPSYASSRRAY
                     VRVAEAVGRPPFTVTYGKKSARAESYGKLREQALELAKEG"
BASE COUNT          877 a         1717 c         1795 g          822 t
ORIGIN      
        1 acaagtcgcc atgtacctct cgcgggagct caccgactcg tcgctgccga agatcgggaa
       61 ggagttcggc ggtcgtgacc acaccactgt cattcacgcg acgtcgaaga tcacgcgcat
      121 gatcaacgag gaccgcactg tgtacaacct ggtgcaagag ctcacagctc gcatcaagca
      181 gcaccgctga ctgtgattct ttcttccttt gcctctccgc cctgttggac cacgtcgctt
      241 tctccccagc gagctcccac agcgcaatcg gtctccgtga ccgcgatcgc gtccgtcttc
      301 cccgccatcc acactcccta cgtactacta cgagaggtct tttaagatca tggaagtcga
      361 aaccaccgtg gaaaccgggc tcgagatccg ttgtgaccgc gacgagctgg ctgcgcgtct
      421 cggactggtc gcccgtgcgg tttccacgcg cggaacggtg caggtgctcg gcggcgtgct
      481 gatccgcgcc caggagggat cgctcgagct ggccgggacg gacatggaac tctccctgcg
      541 cacgtcgctg cccggcgagg tcgacggaga aggcgctctc gtcgtgcccg gccgcttgct
      601 cgtcgatctc gtgcgcctcc tgccggcgtc cgaagtgacc atcgcgtatc ggccggagga
      661 cggagcggcg catgtgacgt ccggcagcta cgcgtcccgc gtcaacgtgt actcggcgga
      721 cgacttcccg cgcctgccga cagtcgacat tccgctccat gctgtcgatc gcgagtcgct
      781 ggtcgggacg atcgaacgag tgtccaaggc tgcgtctcgt gacgagtccc ggccggtgct
      841 cactgggatc ctcgttcgct tcgaggggca gcagctgatc atggccgcaa ccgactcgta
      901 tcggatggcg gtcaaggaga cgccgctcgc ggagtcgggg cccgatctcg acgcgatcat
      961 ccctgcgcgg gcactctcgg aactgacacg ctttgccgtt ggagacaagg tgtcgctcgg
     1021 agtccaggag aaccatgtcg tcttcggggt ggacgacgtc tggctcacga cccgccggat
     1081 cgacggccag ttcccgaact acaagcagct ccggcccgag agcttcgagg tcgagctcga
     1141 cctaccccgc ggcgagctgc tcgaggtcgt gcgccgtgcg gccgtactcg cgcagcgcaa
     1201 ctcgcccctc aggctccgct ttgccgaggg cgagctatcg gtttctgcgc agacccagga
     1261 cgtgggcgag acgagcgaga gcctgccggc cgcgtacacg ggcgagccgc tcgagatcgg
     1321 cttcaacgcc gacttcctgc gcgagggact cgagtccgta tcgggggacg aagtgcggct
     1381 cagcctcatc aacccgctcc ggccggggct gatcacctcg gagggcgacc cgttctggta
     1441 cctgatcatg ccgatccggc tcgccggctg atcgtcaggc ggacacggct cgtcaactat
     1501 cgctcctacg cggagctcga gctcgcgctc cgaccgggac tcgtcctcgt cgtaggcgag
     1561 aacggggctg ggaagaccaa cctgctcgag tcgctccatg tcggaacgca ggggttctcg
     1621 ccgcgaacga ggcacgacgc gcagctcatt cgtttcggag ccgatcaggg agcggtgtcg
     1681 atcgacggcg agcgcggcgg aatctcgacg aggacggaga tcgttctgag tctgcaggag
     1741 ccgaaacgcg caacgctgaa cggcgcccgc ctccgcgccg ccgagcagct ccgcagcgag
     1801 gtgtcgacgc tcgtgttcac gcccgaccgg ctcaccgtcg tgaagggcgg gcccgccgct
     1861 cgtcgggcct acttcgatcg aacgctgggg cggctgctgc ccggccgcgt ctcgctgcct
     1921 gccgagtacg cgtccgccct cgtgcagagg aacgccgctc tgaggcggat cggcgcgggc
     1981 ctctcgacgc gtgaagcgct tgctccctgg accgagcgcg ttgccggtct cggcgcggat
     2041 ctcgtggcgt ctcgggcgga agcgctcgcg gtgctctcgc ctcgcttcgc cgagcaggcg
     2101 gagatgctgg ggctcgccgc agccgagctc tcctatgagg gcgagccgcc ctcgaccgcc
     2161 gtgctcgagg cgagactgga gcgcgatctc gatcgcggca cgaccggtgc cggcccgcat
     2221 ctcgacgacg tcgccgtact cgccgcgtcg cgtgatttgc gtcgcttcgg gtcgcaagga
     2281 gagcagcgca tcgcagttct ctcgctgctg ctcgccgagg cggctctcgt gggtgagcga
     2341 caaggcgttc ccccgctcct gctcctcgat gacgtcttgt cggagctcga cgcccggcgc
     2401 agagcggcgc tgggcgagcg catccgtgga ctcgggcaga ccgtcgtgac ggcgacgagc
     2461 cgggatgcca tgccggttgt gccggatcaa gtgctcgacg tcacccccgg acaagtccgc
     2521 caggccgcct gatgcgccgc ctcggagacg atgcccgccg tgtgcttcgt gaggcgggga
     2581 tgccggaggc gggcgagctg gcagagatca cccgggcctg gcccgccgca gtcggcgacg
     2641 caatctcgcg ctcggcctgg ccgtcccgcg tcgggcggga tggcaccttg cacgttgcca
     2701 cggtctcggc gacgtgggcg ttcgagctgg atcggctctc gggcgagctc gcagagcggc
     2761 tgaccgaggc gctgggcgcg cgcgctccgc gggcacttcg cttcgcgccc ggcaacgttc
     2821 cggctccggc cgcggagccg accggtcgag cgtctcgcgc acggccggag cccggtgagc
     2881 gggagcgacg cgagggggcg agcctggccg aagagatctc ggacgacgag ctgcggaagc
     2941 tcgtcgcgag agccgcctcg gcgagtctcg ccaaagggcg ggacgaccac cgcttctgat
     3001 agacttagtt gagcccgcaa aatgctgatt ttgcagggct tttttcatga ctgacacctc
     3061 gtataccgca aaagacatta ccgttttgga gggtctcgag ccggtgcgct tgcggcccgg
     3121 gatgtacatc ggctcgacgg gcccgcgggg gctccaccac ctcgtctacg aggtggtcga
     3181 caatgcggtc gacgaagccc tcgccggacg aaacgacctc gtcgacgtca ccatccaccc
     3241 ggacaactcg gtgacggtgc gcgacgcggg agcgggcatc ccggtcgacg tcatcgagga
     3301 tcagggtctg tccgccctga ccgtcgtgct gacgaagctc cacgcgggcg gcaagttcgg
     3361 cggcgagggg tacaaggtct cgggcggact gcacggggtc ggcgtctccg tcgtgaacgc
     3421 cttgtcggag tggctcgtcg ccgaggtcag gcgcgacggg cggctttatc gccaggagtt
     3481 cgcccgcggt gccccgaccg gggagatggc ggacggtggc aaggcggacg ggagcgggac
     3541 gacgatctca ttccttcccg accgggaggt gttcgagacg atcgagctgt cggcgacgac
     3601 gcttgtccag cgcctgcggg agacggcatt cctcacgcgt gggctcagaa ttgtcctccg
     3661 cgacgagcgg gccggcggaa agaccgagga gttccactac gagggcggga tccgcgactt
     3721 cgtcgcgtac gtcaacgaca cgaaggacgc ggtgcacaag aacgtgatct tcctcgaggg
     3781 cgagagcgac gatggacagg tcgaggtcgc aatgcagtgg aactcgtcgt acgtcgagtc
     3841 cgtgttctcc ttcgcgaaca acatcaacac caccgaggga ggaacccatc tctcgggctt
     3901 ccgcgcggcg ctgaccggaa cgctcaaccg atatgcgcgc gacaaggggc tgctgaagga
     3961 gaaggaggac aacctcgagg gcgaggacgt gcgagagggc ctcgccgccg tgatctcggt
     4021 caagctccgg agcccgcagt tcgagggcca gacgaagacg aagctgggga acccgccgat
     4081 cgaggggctc gtcaagacga ccgtcaacca gcgactcgcg gagttcctgg aggaaaaccc
     4141 gactcaggcg cgccagatca tcacgaaggc gatctcggct gcgcgggcgc gccaggcggc
     4201 acggaaggcg cgtgagctca cacgccggaa gagcgcgctc gagaactcgg ctctcccggg
     4261 gaagctcgcg gactgcacga tcaaggatgc ggactcggcg gagctcttca tagtcgaggg
     4321 agactcggcc ggaggctcgg ccaagcaggc ccgagaccgc acctaccagg cgattctgcc
     4381 gcttcggggc aagatcatca acagcgagaa gaaccgcatc aacaaggtgc tctcgaacac
     4441 cgagatccaa gcgatgatca ccgccatcgg cacgggcttc ggcgacgagt tcgacatcgc
     4501 caacatccgc tacggacgaa tcatcgtgat gaccgacgcc gacgtcgacg gcgcccacat
     4561 ccgaacgctc gtgctgacgt tcctgtatcg cacgatgccc gagctgttcg agcggggcca
     4621 tgtgtacatc gccctgcctc cgctgtacca cgtgaagctc ggcaaccggg gcatgtactt
     4681 cgagaaggac gcccagctcg aggaactgct cgtccgggag cgcgtcaccg agatgaccgt
     4741 cgccgatcgc gacggcggag agcagagcct gagcgaggcg cgctgggcgc gtttcgtacg
     4801 tgacctcgcg cagttcgagg cctggtcggc gaagctggag tccgagttcg gcgcggccgc
     4861 cagattcgcc gtcgagcacc gattgatcga gacggaaggg gacgagtcga cagacgtcga
     4921 gtcgctgatc gacgccgtgc ccccgaacgg gtacgagctg accgtcgacg agcggctcga
     4981 ggatcggatc aaggtcaagg tggtcgacgc ggagacgaac gccgcccggt tcgtcgagat
     5041 cccgggcgcg atgttctcgg caccgtccta cgcgtcgagc cgccgcgcct acgtccgcgt
     5101 ggccgaggcc gtcggcagac cgccgttcac cgtcacctac gggaagaagt ctgcgagggc
     5161 cgagagctac ggcaagctgc gcgagcaggc gctcgagctc gcgaaggagg g
//
LOCUS       DBNGNK010000269         6159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_619734,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000269 DBNGNK010000000
VERSION     DBNGNK010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6159
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_619734"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="AC150V_07925"
     CDS             <1..160
                     /locus_tag="AC150V_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018151404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3762888.1"
                     /translation="DGLAAFAPTVPVGRNGEPEDIASVFHFLASEEAAYVTGQTIVVD
                     GGATLGLG"
     gene            160..1170
                     /locus_tag="AC150V_07930"
     CDS             160..1170
                     /locus_tag="AC150V_07930"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR3762889.1"
                     /translation="MPARRLGRSGPLVSAIGLGCMGLSEFYGPSDDAGSIQTIRHAID
                     RGVTLLDTADIYGSGHNERLVGAAVAGRRDEIVLATKFGHLRDDRGRFRGISGRPEYV
                     REACEASLARLHVETIDLYQQHRVDPETPIEETVGAMQELVAEGKVRHIGLSEARPED
                     LRRAAETAPIASVQSEYSLLERDVEEDVLRVCEELGIGFLAYAPLVRGLLGGRIRARE
                     DLHENDTRRGGRYPRVAPANLDANLELVAVIEEVAARHRATASQVALAWLLSRAPWIV
                     PIPGTRRSQYVDDNVAAAELELSPEDELRLGELASQARGDRYDSPGRTPTWVSPPARG
                     TR"
     gene            1210..1611
                     /locus_tag="AC150V_07935"
     CDS             1210..1611
                     /locus_tag="AC150V_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3762890.1"
                     /translation="MATNVASLTRDQLAAIPDSYFKAVDAGDVDAILSHFSEDATLTV
                     QTDQVTFTGAEEIRRMFADFVDNSASIFHEIKNIVVEPEAGKVATEQSYIGELKDGSK
                     NDMHNCNFFDVGADGKFTRVIIWMAGSNPLK"
     gene            complement(1675..2409)
                     /locus_tag="AC150V_07940"
     CDS             complement(1675..2409)
                     /locus_tag="AC150V_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR3762891.1"
                     /translation="MRRTTTLLTALALAVSLGVGLAATSISASPNRGGAEKTVPFAKT
                     KLFVEMNATDGDAGMQLDLDGEDWKRLSVEGPAGQTILDVTGRANLEGYGLTGLTFES
                     SEPPFDRHPLSKFKARFPEGVYRFSGETVDGRKLAGKTRFTHDFPPGPVVTAPEEDAV
                     LSRDSVVVAWKPVAKPAGIKVIAYQVIVTQEKPVRVFQVDLPATARSQTVPADFLAPQ
                     TDYSVEVIVRESSGNQTITEVPFRTG"
     gene            2582..2830
                     /locus_tag="AC150V_07945"
     CDS             2582..2830
                     /locus_tag="AC150V_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762892.1"
                     /translation="MKVLERVRARRRAARLSRMEKSAAAHDEFDRELVEHDVAGMRED
                     TFAASGNGVVVPGLPTSTPRDVYTEFEHDEEEPVDPAP"
     gene            complement(2848..3114)
                     /locus_tag="AC150V_07950"
     CDS             complement(2848..3114)
                     /locus_tag="AC150V_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762893.1"
                     /translation="MGDSRGPGATARVFLADGTDLGVAHLPAPIQPGDLCAVADGLPL
                     RVISVAPTLPDSPFDALIEVIWERRHLFRRGEGNPVPRRPSARD"
     gene            3526..4056
                     /locus_tag="AC150V_07955"
     CDS             3526..4056
                     /locus_tag="AC150V_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3762894.1"
                     /translation="MAPRRERSARGHAANAQPRDPGKRRKLAPLAIVGVGYNSLWERG
                     RRNYAHWAAEFDRQAGRLVRTLRRQGAEQVVWITLRQSRRTVIPSGGLWQFDNYAWYF
                     PYVNERLRKLASSRRDVVLADWAAVSDRRGITYDAIHLNPGGARLMARTVRSAIEHEG
                     RAQTRRSAKPAGCVFR"
     gene            4100..5107
                     /locus_tag="AC150V_07960"
     CDS             4100..5107
                     /locus_tag="AC150V_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR3762895.1"
                     /translation="MSFDRPFVLLALLSAPLAVGLYFFAQRRSRPFAVVYPNLAVLAT
                     VVSGSRRRGHLPPVLWLVVLFMLVLAVAGPKLTRTAPVKDATVVLAVDTSKSMLSDDV
                     LPTRLDAAKQAARTFVEQVPDSFRVGVVVFAGDVAVGALPTSDRAVVNAAIDAIVPRE
                     SSGTAIGDALARSVEVGILPASDGAGQPTATGGQQSIAPASILFLSDGRQEDGLLRPL
                     EGSARARAAGIPVHTVALGTEGGARQLGPTGAGFFYDNLGTFRVPDLETMRTIAYTTG
                     GEFVEARTADALSRAYESLGSRLGRVQRISDVTVAFVGFAAVGLLVAAFLSALWWPRV
                     P"
     gene            5131..5481
                     /locus_tag="AC150V_07965"
     CDS             5131..5481
                     /locus_tag="AC150V_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762896.1"
                     /translation="MNEHTRRAVAYIAGCIVSGEASGSLYDHETERDYEFPGEVSTDY
                     VAVRDRDRGCDVGGILPSLYHHGHGNHLTLRIRGSAFSGHDYGSGHDYEGWVLGRDIS
                     INDHQTGEEHRFTI"
     gene            complement(5536..5608)
                     /locus_tag="AC150V_07970"
     tRNA            complement(5536..5608)
                     /locus_tag="AC150V_07970"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5573..5575),aa:Trp,seq:cca)
     gene            5672..5842
                     /locus_tag="AC150V_07975"
     CDS             5672..5842
                     /locus_tag="AC150V_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762897.1"
                     /translation="MQRSALGFLFLALAAGLFLVAIFSALEGGRAWVIAVAATVIGLW
                     LLDLGRRGIRRN"
     gene            5876..>6159
                     /locus_tag="AC150V_07980"
     CDS             5876..>6159
                     /locus_tag="AC150V_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3762898.1"
                     /translation="MADASGDTHTLWQEYRRTNDRELRDRLILTYAPLVKFVAGRLGA
                     SLPAHVDEQDLVSYGLLGLIGAIERFDPDREIKFETYAISRIKGAIIDELR"
BASE COUNT          967 a         2030 c         2158 g         1004 t
ORIGIN      
        1 ggacggcctc gccgccttcg caccgaccgt tccggtcggg cgaaacgggg agccggagga
       61 catcgcttcg gtcttccact tcctcgccag cgaggaggcg gcctacgtca ccggccagac
      121 gatcgtcgtg gacggtggcg cgacgctcgg cctcggatga tcccggctcg gcgcctcggg
      181 cgctcgggcc cgttggtctc ggcgatcggc ctcggttgca tggggctgtc cgagttctac
      241 gggccgtcgg acgacgccgg gagcatccag acgatccgcc acgcgatcga tcgcggagtg
      301 acgcttctgg acacggccga catctacggc agcgggcaca acgagcggct cgtcggcgca
      361 gccgtggccg gtcgcagaga cgagatcgtc ctcgcgacga agttcgggca cctgcgggac
      421 gacagaggca ggttccgcgg gatcagtggc aggccggagt acgtccgaga ggcctgcgag
      481 gcgagcctcg cgcgactcca tgtcgagacg atcgacctct accagcagca tcgcgtcgac
      541 ccggagacgc cgatcgagga gaccgtcgga gcgatgcagg agctggtggc ggagggaaag
      601 gtgcgccaca tcggcctctc ggaggctcgc cccgaggacc tgaggcgcgc cgccgagacc
      661 gccccgatcg cgagcgtcca gagcgagtac tcgctgctcg aacgtgacgt cgaggaggac
      721 gtgctacggg tgtgcgagga gctggggatc gggtttctcg cgtacgcacc actcgtgcgc
      781 ggacttctcg gcggccgcat ccgggccagg gaggatctcc atgaaaacga cacgcggaga
      841 ggtggacgtt atccgcgggt ggcgccggcg aacctcgacg cgaacctcga gctcgtcgcg
      901 gtgatcgagg aggtcgcggc acgtcacagg gccaccgcct ctcaggtcgc gctcgcctgg
      961 cttctgtcac gggcgccctg gatcgtcccg atccccggga cgcgccgcag tcagtacgtg
     1021 gacgacaacg tcgcggccgc cgagctcgag ctgtccccgg aggacgagtt gcgactcggc
     1081 gagctcgctt cccaggcgcg gggcgatcgc tacgactcgc ccggccggac gccgacgtgg
     1141 gtgtcacccc cagcccgcgg gacgcgctag gctgctgcca gaccgatgtc agacattcaa
     1201 ggaggctcga tggccaccaa cgtcgcttcg ctcacgcgtg atcagctcgc cgccatcccg
     1261 gacagctact tcaaggcggt tgacgccggc gacgtcgacg cgatcctgtc gcatttctcc
     1321 gaggatgcga cgctgaccgt ccagaccgac caggtcacgt tcaccggcgc cgaggagatc
     1381 cggcgcatgt tcgccgactt cgtcgacaac tccgcgtcca tcttccacga gatcaagaac
     1441 atcgtcgtgg agccggaagc ggggaaggtc gcgaccgagc agagctacat cggagagctc
     1501 aaggacggct cgaagaacga catgcacaac tgcaacttct tcgatgtcgg ggccgacggg
     1561 aagttcaccc gcgtcatcat ctggatggcg ggctcgaacc cgctcaaata gcctgtttgc
     1621 tgctcgcggg gcccggtcga ccgggccccg ctgctagctg ccggccctga tcgtctagcc
     1681 ggtcctgaac gggacctcgg tgatggtctg gttcccgctc gactcgcgca cgatcacctc
     1741 gacgctgtag tccgtctgcg gcgcgaggaa atctgccggg acggtctggc ttcgtgccgt
     1801 tgccggtagg tcgacctgaa acacgcgcac gggcttctcc tgcgtgacga tcacctggta
     1861 ggcgattacc ttgatcccgg ccggcttcgc aaccggcttc caggccacga cgacgctgtc
     1921 gcgcgagagc acggcgtctt cttccggagc cgtgacgacc gggccgggcg gaaagtcgtg
     1981 ggtgaacctg gtcttgcccg cgagcttccg cccgtcgacg gtctcgccgg agaaccggta
     2041 cacgccctcc gggaagcgcg ccttgaactt cgagagtgga tgccggtcga agggcggctc
     2101 gctgctctcg aacgtcaggc cggtcagccc gtagccctcc aggttcgccc tgcccgtgac
     2161 gtcgaggatc gtctgcccgg ctgggccctc gacggacaga cgcttccagt cctcgccgtc
     2221 gagatccagc tgcatcccgg cgtcaccgtc ggtcgcgttc atctccacga agagcttcgt
     2281 cttcgcgaac gggacggtct tctccgcgcc gcctcgattc gggctcgcag agatgcttgt
     2341 cgcggcgaga ccgacaccga gcgagacggc gagcgcgagg gctgtgagga gtgtggtcgt
     2401 gcgtcgcacc tgcgcctcct tggtcgtagt ggaagcgacc gtaggagggc cggatgagac
     2461 gaagatgagg caaatgcagc cgcgaccgga ctcagtactt ccgagcggct gtctgcccgc
     2521 tgccaccgat gccgtgatcg aggagggggg cgatactaga agtgaggcga ggtggtgagc
     2581 aatgaaagtc ctggagcgcg ttcgggctcg gcgccgtgct gcgcgactga gccggatgga
     2641 gaagtcggct gctgcgcatg acgaattcga ccgcgagctc gtcgagcacg acgtggcagg
     2701 aatgcgcgag gacaccttcg cggcatccgg caacggcgtc gtcgttcccg gtcttccgac
     2761 gtccacgccc cgtgacgtgt acaccgagtt cgagcacgac gaggaggaac cggtcgatcc
     2821 ggctccctga gagcgccgtt gggacggtca gtcgcgcgcg gaaggcctgc gcgggacggg
     2881 gttgccctca ccgcggcgga acaggtggcg gcgctcccag atgacctcga tgagcgcgtc
     2941 gaaaggggag tccggtagcg tcggcgcgac cgagatcacg cgcagtggca gcccgtcggc
     3001 gacggcgcac agatcgcccg gctggatcgg agcggggagg tgggcgaccc cgaggtcggt
     3061 gccgtcggcg aggaagacgc gcgcagttgc cccgggcccc ctcgaatccc ccataccctc
     3121 accctaggca atgtcacgtg cctgtcgagt acagggtgtg ggcaggcggc ggcgctccgg
     3181 cgcacggacg catcgctgaa cgagcgatgc tgctgacacg cttcgcgtcc agcgagcggg
     3241 acgatggccg ccatgctggc ccgcgtagaa gtcctcggat tcgatcgggg cggagccccg
     3301 ggcaccccgc ctcggtgaga gctaccttgg aggggtgcgc gtggtggtgg cgcccgtcgt
     3361 gatcgccctc gcgctcgccg gagccggagc gtcgctcggc gaggcggccg aacgctgcgg
     3421 tgtgaagcca gcaaagccgc actggttggc agagcggcgc gcgacggtgc tcgtcgactc
     3481 tgtgttgctg ggcggtgtcg acgcgctgcg cgggaacatg gcgcaatggc gcctcgacgt
     3541 gagcggtcgg cccgcggtca tgctgcgaac gctcaaccga gagatccagg gaaacggcgg
     3601 aaactcgcac cgctcgcaat cgtcggcgtc ggctacaact cgctctggga gcgtgggcgc
     3661 aggaactacg ctcactgggc ggccgagttc gatcggcagg ccggccgact cgtgcgaacg
     3721 ctcaggcgtc agggggccga acaggtcgtc tggatcacgc ttcgccagtc gcggcggacg
     3781 gtgattccga gtggcgggct ctggcagttc gacaactacg cgtggtattt cccgtacgtg
     3841 aacgagcgcc ttcgcaagct cgcgagcagt cgccgcgacg tcgtgctggc cgattgggcg
     3901 gccgtctcgg atcggcgcgg gatcacgtac gacgcgatcc acctcaaccc gggcggcgca
     3961 cgactgatgg ctcgcacggt tcgcagcgcg atcgagcacg aggggcgggc gcagacgcgg
     4021 cgctcggcca agccggcggg gtgtgtcttc cgatgaatgc ggaccgtgtc gatcgtctac
     4081 gcgggcggcg atcgctgcga tgagcttcga ccggcccttc gtgctgctgg cgctgctgtc
     4141 ggcgccgctc gccgtcgggc tctacttctt cgcgcaacgg cgctcgcgcc cgttcgccgt
     4201 cgtctacccg aacctcgcgg tgctcgcaac ggtcgtgagc ggatctcgcc ggcgggggca
     4261 tctgccccca gttctgtggc tggtggtgct gttcatgctc gttcttgccg tagccgggcc
     4321 gaagctgacg cggacggcgc ccgtcaagga cgcaaccgtg gtgctggcgg tcgacacgtc
     4381 gaagtcgatg ctgtcggacg acgtcctgcc gacccgattg gacgccgcca agcaggcggc
     4441 gcgtactttc gtcgaacagg ttcccgacag tttccgcgtc ggcgtcgtcg tcttcgccgg
     4501 cgacgtcgcc gtcggtgcgc tccccacgtc cgaccgcgct gtcgtaaacg cggcgatcga
     4561 tgcgatcgtg ccgagggaga gctcgggaac ggcgatcggc gacgcgcttg cacgatccgt
     4621 cgaggtcggc atcctgcccg cctcggacgg ggcggggcag cccaccgcga caggcggaca
     4681 gcagtcgatc gcgccggcct cgatcctttt cctctctgac ggaagacagg aggacgggct
     4741 gctccggccg ctcgagggct cggcgcgggc gcgcgcggcc ggcattcccg tgcacaccgt
     4801 cgcactcggt accgaagggg gcgcgcgaca gctgggcccg accggcgcag ggttcttcta
     4861 cgacaatctg ggcacctttc gagtgcccga cctcgagacg atgcggacga tcgcctatac
     4921 gaccggcggc gagttcgtcg aagcacggac agccgatgcg ctcagccgcg cgtacgagag
     4981 tctcggcagc cgtctcgggc gtgtgcagcg gatcagcgac gtcactgtcg cgttcgtggg
     5041 attcgccgcc gtcgggctcc tcgtcgccgc tttcctctcg gcgctctggt ggccgcgcgt
     5101 tccttgatcc acttgtcatt cgcccgctga gtgaacgagc acacgcgaag agcagtcgcg
     5161 tacatcgccg gctgcatcgt tagcggggag gcctccggct cgctttacga ccacgagacc
     5221 gagcgcgact acgagtttcc gggcgaggtc tcaaccgact acgtcgccgt tcgcgatcgc
     5281 gacaggggct gcgacgtggg tggcatcctg ccctccctct accaccacgg acacggcaac
     5341 cacttgaccc tgcggatccg aggctcagct ttctcggggc acgactacgg gtcgggccac
     5401 gactacgagg gctgggttct cggccgggac atatcgatca acgaccacca gacgggcgaa
     5461 gagcaccgct tcacgatctg acgcgggctc gctaggcgac gtggcaaaac cccgcctgag
     5521 cggggctttc ttctacaggg gagacaggac tcgaacctgc aacccccggt tttggagacc
     5581 ggtgctctac caattgagct actcccctgg gtcgcggcag ccatggtagc ggggtgaata
     5641 gtcggagtcc ggtacttgcc agaatggagg ggtgcaacgg agtgcgcttg gatttctctt
     5701 cctcgccctc gcggccggcc tgttcctcgt tgcaatcttc tcagctctcg aagggggacg
     5761 ggcctgggtg atcgccgtcg ccgccacggt catcggcctc tggctgctcg acctcgggcg
     5821 gcgggggata cgccgcaact gaggagtttg ccggccgacg tcgtatgctc ttgaggtggc
     5881 ggatgcctcg ggggacaccc acacgctctg gcaggagtac cggcgaacca acgaccgtga
     5941 actgcgcgat cgcctgatcc tgacgtacgc gccgctcgtc aagttcgtcg ccggacgtct
     6001 cggcgcgagc ctgccggcgc acgtcgacga gcaggatctc gtctcgtacg ggctgctcgg
     6061 cctgatcggc gccatcgagc gcttcgatcc cgaccgggag atcaagttcg agacgtacgc
     6121 gatctcgcgc atcaagggcg cgatcatcga cgagttgcg
//
LOCUS       DBNGNK010000270         1980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1264393,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000270 DBNGNK010000000
VERSION     DBNGNK010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1264393"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1159
                     /locus_tag="AC150V_07985"
     CDS             65..1159
                     /locus_tag="AC150V_07985"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3762899.1"
                     /translation="MSEIVRTAFDAPQRSMGGDLVDWEGWYWPNHFGDPVAEHQAVRN
                     DVGVWDASPLRKWRFRGADALRAADHVFTNDMAGLEIGQVRYAPFCDENGKMVGDGTV
                     FRVADDECTVVTALDSDLDHFTRVLDGMALEIDPMTHEAPQLGVNGPRARELVQGLTS
                     ADVSSLGYFRFLPERVEVGGVPCLVSRTGYSGELGYELFCSPDGAEQLWESVVGAGAQ
                     PYGLAAVETIRIESGLVFIGYDYFQGETSPFDVGLDAVIRLDTGDFCGKAALAAESAS
                     PPNRFVTLVVDGPVPEYGAAVSRDGEQVGTLTSPCESPTLDKVIGLTVLRSDLAREGE
                     QVDVAVEGGMAPATVAQLPIYDPDKARPRA"
     gene            1258..>1980
                     /locus_tag="AC150V_07990"
     CDS             1258..>1980
                     /locus_tag="AC150V_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3762900.1"
                     /translation="MARNPKHDILFEPIQIGPKTMKNRFYQIPHCNGFGSEKPFSQAF
                     FRSTKAEGGYAAVSTEYCSISPESDDTHRMSARLWDDDDIKNLSLMCDMLHEYGCLSA
                     AELWYGGPHAPCMETRCVAKGPSQIPSDFEYLTYAIEADKDDIRYLQQLYVDAAKRAR
                     TAGFDIVYVYGSHSYLPQQFLTPFYNKRTDEYGGSFENRARFWRETIEQVKEAVGDDT
                     AIAVRMSTDMFMGEPGTQLKRDC"
BASE COUNT          344 a          674 c          658 g          304 t
ORIGIN      
        1 gggcagctgc cccggggaag tgcaagagtt ccggtcgaag ccgaccacga tcaggggagg
       61 accgatgagc gaaatcgtcc gcaccgcctt cgacgcgccg cagcgctcca tgggtggaga
      121 cctcgtcgac tgggagggct ggtactggcc gaaccacttc ggcgatccgg tcgccgagca
      181 tcaggcggtc cggaacgatg tcggcgtctg ggacgcctcg ccgttgcgga agtggcgctt
      241 ccgcggggcg gacgcgctgc gtgcagccga ccacgtcttc acgaacgaca tggccggctt
      301 agagatcggg caggtgcgct acgcgccgtt ctgcgacgag aacggaaaga tggtcggtga
      361 cggcacggtc ttccgagtcg cggacgacga gtgcaccgtc gtcactgcgc tcgactccga
      421 tctcgaccac ttcaccaggg tgctcgacgg catggcgctc gagatcgacc cgatgacgca
      481 cgaggcgcct cagctcgggg tcaacgggcc gcgtgcgcgc gagctcgtac agggtctcac
      541 gagcgcggac gtctcgagtc tcggctactt ccgcttcctc ccggagcgcg tggaggtcgg
      601 cggcgtcccc tgcctcgtct cccgaacggg gtactcaggc gagctcggct acgagctctt
      661 ctgctcgccg gacggcgccg agcagctctg ggagtcagtc gtcggcgcgg gtgcacagcc
      721 gtacgggttg gcagccgtcg agacgatccg gatcgagtcg ggcttggtgt tcatcggcta
      781 cgactacttc cagggcgaga cgagcccctt cgacgttggc ctcgacgcgg tgatccggct
      841 cgacaccggc gacttctgcg gcaaggctgc gctcgcagcg gagagcgcga gcccgccgaa
      901 ccggttcgtg accctcgtcg tcgacgggcc cgtgcccgag tacggcgccg ccgtctctag
      961 agacggcgag caggtcggca ccctcacgag cccctgtgaa agcccgacgc tggacaaggt
     1021 gatcggcctg acggtgctgc gctccgacct cgcccgagag ggcgagcaag tcgacgtggc
     1081 cgtcgagggc gggatggccc ccgcgaccgt ggctcagctg ccgatctacg acccggacaa
     1141 ggcgcggccc cgcgcgtaga gcgcccggtc ggcgccgcga cagaaaactg cggttgcatc
     1201 cggtcggctg caaccggaga atccagccat ctcacttccc cgaggaggac gagtcggatg
     1261 gcgcgcaacc cgaagcacga cattctcttc gagccgatcc agatcggccc gaagacgatg
     1321 aagaaccgct tctaccagat cccgcactgc aacggcttcg gctcggagaa gcctttcagc
     1381 caggccttct tccgttcgac gaaggcggag ggcgggtacg cagccgtgtc gaccgagtac
     1441 tgctccatat cgccggagtc cgacgacacg caccggatgt cggcccgcct ctgggacgac
     1501 gacgacatca agaacctctc gttgatgtgc gacatgctcc acgagtacgg ctgtctctcg
     1561 gcggccgagc tctggtacgg cggcccccac gcaccctgca tggagacacg ctgcgtcgcc
     1621 aagggcccgt cgcagatccc gtccgacttc gagtacctga cgtatgcgat cgaggccgat
     1681 aaggacgaca tccgctacct gcaacagctc tacgtcgacg ccgccaagcg ggcgcggacg
     1741 gccggcttcg acatcgtcta cgtctacggc tcgcactcct acctccctca gcaattcctc
     1801 acccccttct acaacaagcg caccgacgag tacggcggct ccttcgagaa ccgcgcccgg
     1861 ttctggcggg agacgatcga gcaggtgaag gaggccgtcg gcgacgacac cgcgatcgcc
     1921 gtgcgcatgt ccacggacat gttcatgggc gagccgggaa cgcagctcaa gcgcgactgc
//
LOCUS       DBNGNK010000271         4120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1839875,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000271 DBNGNK010000000
VERSION     DBNGNK010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1839875"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="AC150V_07995"
     CDS             <1..687
                     /locus_tag="AC150V_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011601433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR3762901.1"
                     /translation="ARDGSLRAEGVHGFWPASADGDDLVVAGTRIPMLRQQAAYGDSR
                     PNRSLADFVAPAASGVADHVGGFAVAIHGADELASRYEAEHDDYHSIMTRALADRLAE
                     AFAEWLHEQSRRAWYEPEERLSSGELIAEKFRGIRPAFGYPACPDHSEKRRLFDLLDA
                     ESAGIALTETFAMTPPAAVSGLYLGHPHARYFSVGRIGRDQVEDYARRKGVDVGEAER
                     WLRPNLAYEP"
     gene            831..2237
                     /locus_tag="AC150V_08000"
     CDS             831..2237
                     /locus_tag="AC150V_08000"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762902.1"
                     /translation="MPGSTTGRLNLLRRAPSFRLLVLAAVGSGLGTYLAAIALAVDIF
                     DRTGSGKWLAALLIADFLPIVLIGLTLGPLVDRLSRRRLMIVSDVVRAAVFCALPFVD
                     SPLGIVVLAGITGIATGFFRPAVYAGLPNLVEDDDLADANSLLAGIENLAWMVGPIVG
                     GVLLAASGPSLAYLINAVTFVVSAALVVRIPAGRLQSEESLSRGHWRDVRDGLSLVRG
                     SLQLLTVLIVWNIVLVGNAAVNVSEVIFAKDSLGTGNVGLGILIGATGLGLTIGSFLA
                     PSLLATVGLRRGYAGSIALMGLGWGIASFAPALWAVLPLVVVATIGNGCALVCNQLLL
                     QRGAPDKLRGRVLAVVMATFYTTLGIGMVIAGPLVDALGGRTVWAIAGVVYLGAAVIA
                     AILTSRIRADALAASPPPEEKRIVAAPEPVSALDAGDANGDLERAEPLILRADGHKRL
                     VTLLGEVEEVRRAESERT"
     gene            2273..3262
                     /gene="meaB"
                     /locus_tag="AC150V_08005"
     CDS             2273..3262
                     /gene="meaB"
                     /locus_tag="AC150V_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR3762903.1"
                     /translation="MARKEWTHEALVAGVRDGDRRALARAISLVENRDPEAYAVVREL
                     YAETGSAYTIGVTGPPGVGKSSLIGALVRHVRELGLTVGVVSVDPSSPFSHGALLGDR
                     IRLSDHFLDPGVYIRSMGTRGHLGGLAEATLQAMLLLDAAGKDLVFLETVGTGQSEIE
                     VITIADSVLLVLMPGSGDSVQALKAGIMEIPDVIAINKSDHPAANTMVTEIRSVLSLD
                     RARAWMPPIVLTEALRGEGVAELWRGLEEHRAHLEADGGLDARRRSNLAAEVFALASS
                     RAKEHLERRVADDPELRRLLDLVQRRELDPLTAVREIMVEVFDVDDRSATNDR"
     gene            3228..>4120
                     /locus_tag="AC150V_08010"
     CDS             3228..>4120
                     /locus_tag="AC150V_08010"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR3762904.1"
                     /translation="MSTTGLQPTIADVRAARERLAGVARTTPLSSSETLSRLTGRPVL
                     LKAENLQRTGAFKIRGAYNTIGQLDRSERAAGVVTASAGNHGQAVAWAARRVGIPATI
                     FVPEGAPMAKVEAARGYGATVRFEGDDYEETVDAARAFERGSGATFVHAFDDERVIAG
                     QGTVGLELADQLPQGPGTVIVPVGGGGLAAGVAVALRAERPELKVIGVQAEACAPLAG
                     LAPSGPTIADGIAVKRPGEKTAAILRDLLDEIVVVDDESISLAIVLLLERAKLVVEGA
                     GAAPLAALLAGRVAGEDRACAV"
BASE COUNT          546 a         1402 c         1478 g          694 t
ORIGIN      
        1 gcccgcgacg gctcgttgcg ggcggagggc gtgcacggct tctggccggc gtccgccgac
       61 ggcgacgacc tggtcgtcgc cgggacgcgt atcccgatgc tgcgtcagca ggcggcctac
      121 ggcgactccc gtccaaaccg gtcgctcgcc gatttcgtcg ctcccgccgc ctcgggggtc
      181 gccgatcacg tcggcggctt cgcggtcgcg atccacggtg cggacgagct cgcgtctcgc
      241 tacgaggccg agcacgacga ctaccactcg atcatgacgc gggcgcttgc cgaccgtctc
      301 gccgaagcgt tcgccgagtg gttgcacgag cagtcgagac gtgcctggta cgagccggag
      361 gagcggctct cgagcggcga gctgatcgcc gagaagttcc gtggcatccg gcccgcattc
      421 gggtacccgg cctgccccga tcattccgag aagcggcggc tcttcgacct gctcgacgcg
      481 gagtccgccg gaatcgcgct cacggagacg ttcgcgatga cgccgcccgc ggccgtcagc
      541 ggtctctatc tcggccaccc gcacgcccgc tatttttcgg ttgggcgaat cggtcgcgat
      601 caggtggagg actacgcgcg gcggaagggc gtggacgtgg gcgaggccga gcgctggctg
      661 cgcccaaacc tcgcttacga gccgtaatcc gcggaaagag gtcgcctcga gtccgcgttg
      721 aacaaacacg gatcgatggt ctggcccctc tctcgatctg acgggtccct ctcggtcacg
      781 cccgtttggg ctcttgtaga cccctccggt attggtggta ccttcggctc gtgcctggga
      841 gcacgaccgg ccgtctgaac ctccttcgcc gagcgccgtc cttccggctg ctcgtgctgg
      901 cggccgtcgg gtcgggcctc ggcacgtacc tggctgcgat cgcgctggcg gtcgacatct
      961 tcgatcgcac cgggtcgggc aagtggctcg ccgcgctgct gatcgcggac ttcctgccga
     1021 tcgtgctgat cggcctcacg ctcggtccgc tcgtcgaccg gctctcgcgc cgccggctga
     1081 tgatcgtctc cgatgtcgtc cgggcggcgg tcttctgtgc gctgcccttc gtcgactcgc
     1141 cgctcgggat cgtggtactg gcgggaatca ccggcatcgc caccgggttc ttccggccgg
     1201 ccgtctatgc ggggctgccg aacctcgtcg aggacgacga tctcgccgac gcgaactccc
     1261 tcctcgccgg catcgagaac ctcgcctgga tggtcggccc gatcgtcggc ggcgtcctgc
     1321 tcgccgcgtc ggggccgtca ctcgcgtact tgatcaacgc ggtgaccttt gtcgtctccg
     1381 cggcgctcgt cgtccggatt cccgccggca ggctgcagtc ggaggagtcg ctctcgcgtg
     1441 gacactggcg cgatgtccgc gacgggctct cgctcgtgcg aggctcgctt cagctgctga
     1501 ccgtgctgat cgtctggaac atcgtcctgg tcggcaacgc cgcggtcaac gtctccgagg
     1561 tcatcttcgc gaaagactcc ctgggcacgg gcaacgtcgg gctcgggatc ctgatcggcg
     1621 cgaccggtct cggcctcacg atcgggagct tcctcgcgcc gagcttgctc gcgacggtcg
     1681 gcctacgccg cggctacgcg ggctcgatcg cgctgatggg tctcggctgg ggaatcgcgt
     1741 cgttcgctcc cgcgctgtgg gcggtgctcc ctctggtcgt cgtggccacg atcgggaacg
     1801 ggtgcgcgct cgtctgcaac cagctgctgc tgcagcgcgg cgcgcccgac aagctccgcg
     1861 gccgtgtgct cgccgtcgtg atggccacct tctacacgac gctcgggatc ggaatggtga
     1921 ttgccgggcc gctcgtagat gctcttggcg ggcgcaccgt ctgggcgatc gcaggcgtcg
     1981 tgtacctcgg cgccgccgtc atcgccgcga tcctgaccag ccgcattcgc gccgacgcgc
     2041 tcgccgcctc gccgccgccc gaagagaagc ggatcgtcgc ggctcccgag ccagtttcgg
     2101 ctcttgacgc cggcgacgcg aacggcgacc tcgagcgagc agagccgctg atcctgcggg
     2161 ccgacgggca caagcgactg gtcactctcc tcggcgaggt cgaagaagta cgccgcgccg
     2221 agtccgagcg aacctgaggc cgcagccgtc cggctgaata gagtgcgcat tcgtggcgcg
     2281 caaggagtgg acgcacgagg cgctcgtggc cggcgtgcgc gacggcgatc ggcgggcgct
     2341 cgcccgcgcg atctcgctgg tcgagaaccg tgatccggag gcgtacgcgg tcgtccgcga
     2401 actgtacgcc gagacaggca gcgcctacac gatcggcgtg accgggcccc cgggcgtcgg
     2461 caagtcgtcg ctgatcgggg ctctcgtccg gcacgtccgc gagctcggcc tgacggtcgg
     2521 agtcgtctcc gtcgacccgt cgagcccgtt ctcgcacgga gcgcttctgg gcgaccggat
     2581 ccggctctcc gatcacttcc tcgatcctgg ggtgtacatc cgctcgatgg gaacgcgcgg
     2641 ccatctcggc gggctcgccg aggcgacgct gcaggcgatg ctgctgctcg acgcggcggg
     2701 aaaggatctc gtctttctgg agacggtcgg gaccgggcag agcgagatcg aggtgatcac
     2761 catcgccgac tcggttctgc tcgtcctcat gccgggatca ggcgactccg ttcaggcact
     2821 gaaggccggc atcatggaga tcccggacgt gatcgcgatc aacaagtcgg atcatccggc
     2881 cgcgaacacg atggtcacgg agatccgctc cgtgctgtcg ctcgaccgcg cgcgtgcatg
     2941 gatgcccccg atcgtgctga cggaggctct tcgcggcgag ggggttgccg agctgtggcg
     3001 cgggctcgag gagcatcgcg cgcatctcga agcggacggc gggctcgatg ctcgtcggcg
     3061 gagcaatctg gcggccgagg tcttcgcgct ggcctcgagt cgcgccaaag agcacctcga
     3121 gcggcgcgtg gcagacgatc cggagctccg gcggctgctc gacctcgtcc agcgtcgtga
     3181 gctcgacccg ctgaccgctg ttcgcgagat catggtcgag gtattcgatg tcgacgaccg
     3241 gtctgcaacc aacgatcgct gacgtccgcg ccgcgcgcga gcggctcgcc ggtgtcgcac
     3301 ggacgacgcc tctgtcgagc tcggagacgc tgagccgtct gaccggtcgc cccgtcctgc
     3361 tcaaggccga gaatctccag cgaacgggtg ccttcaagat ccggggcgcc tacaacacga
     3421 tcggccagct ggatcgttcc gagcgtgcgg cgggggtggt tacggcaagc gcgggcaacc
     3481 acggtcaggc cgtcgcctgg gccgcgcgtc gggtggggat accggcgacg atcttcgtgc
     3541 ccgagggcgc gccgatggca aaggtcgagg ccgcgcgcgg ctacggggcg accgtccgct
     3601 tcgagggcga tgactacgag gagacggtcg acgcggcccg ggccttcgag cgtggcagcg
     3661 gtgcgacctt cgttcacgcc ttcgacgacg aacgcgtcat cgccggacag ggaacggtgg
     3721 ggctggaact ggcggatcag ctcccgcagg gccccgggac ggtgatcgtt cccgtcggcg
     3781 gtggcgggct ggccgcgggg gtcgcggtcg ccctccgagc cgagcggccg gagctgaagg
     3841 tgatcggcgt gcaggccgag gcatgcgcgc cccttgcggg cctggcgccc agcgggccga
     3901 cgatcgcgga cggcatcgcg gtgaagcgcc cgggcgagaa gacggcggcg atactgcgcg
     3961 atctgctcga cgagatcgtc gtcgtcgacg acgagtcgat ctccctggcg atcgtgctcc
     4021 tgctggagcg ggcgaagctc gtggtcgagg gcgcgggggc ggctcccttg gcggcacttc
     4081 tggcggggcg ggtcgcgggt gaagatcgtg cctgtgccgt
//
LOCUS       DBNGNK010000272         3666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_120194,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000272 DBNGNK010000000
VERSION     DBNGNK010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_120194"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="AC150V_08015"
     CDS             <1..1143
                     /locus_tag="AC150V_08015"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3762905.1"
                     /translation="LLPLDAQPARMTTGASLEGATPRETLERLLEVVDLEALRREQRA
                     REPGEPRLGIGLATLVEPAPGPPDFGAATGFPVPPERAHARVEPDGRLTVFTAQHPHG
                     QGHETTLTQVAADELGVPLDHVRVVHGDTQDSPFSLLGTSGSRAATRASGAALYAVRD
                     VRRQVLEIGGALLEISPDDLEITEGVVRAKGAGTRRLTLADVALAAYLGSPALPPSHA
                     IGALEAGWTLDDPPGGWSSASHLCVVEVDPETGIVGIVRYVVVHDCGTMINPAIVEGQ
                     VRGGVAQGIGGALLEESAYDDDGQFLAGTLMDYLLPTAMDVPPIEIVHLESAPLAEVS
                     FRGVGEGGAIGAPPAVTNAVADALGADLDELPLTPTRVLQALGVLP"
     gene            1140..2096
                     /locus_tag="AC150V_08020"
     CDS             1140..2096
                     /locus_tag="AC150V_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3762906.1"
                     /translation="MSRPVLPEWSGVTLRYLSPRDTINRFGWQSPSQRDGLLPGEARA
                     FSEIEQRLPRQDGPVTTTKRRFANALEATETTKTLARRLGAQMVGVTPVDQEYVYEGR
                     SVKHRYAVIVAVAMDYDEILELPRAETQEEYIRVYNDCSRIAVELAEWIRKNGYPARA
                     HTLRDEEIAMLPHAQAAGLGELGKHGSLINRELGCSFRLAAVTTDLPLVTDAPRDEGI
                     EDFCRSCRMCTVYCPGDAIDGEKALVRGVEKWVVDTERCAPYFGSHYACGICIQVCPF
                     NAKAFDGMFRDHYVETMQELDAEELAEQLTSTLVPLASPKLD"
     gene            complement(2093..2776)
                     /locus_tag="AC150V_08025"
     CDS             complement(2093..2776)
                     /locus_tag="AC150V_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3762907.1"
                     /translation="MAGEAVAALREAIIVGDLPPGSPLRLEELAASLDMSISPIREAI
                     RYLETVGLAEHVAYRGARVTELNGEDMRDVYEVRSALEVSAVRRAAVRFTEGDDAALG
                     GVLADLDAAYERGDRRAIVRGNTVFHTQLARASGSRWIERLLLPTFETTERYGAAVLR
                     AGRPKETMAIEAEGHSEILDALRAHDSDAAEQAMRRHLAVFSDTFDSLVRELVGEQEP
                     PAAASAASS"
     gene            3012..>3666
                     /locus_tag="AC150V_08030"
     CDS             3012..>3666
                     /locus_tag="AC150V_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR3762908.1"
                     /translation="MTSPAAIAGVGESMYMRRPDGDLSTPVVLAAAARTALEDAGLAP
                     ADVDGLGVSSFSLAPDRAIDMAVRLGVRARWLMDGATGGASAIDMLQHARRAVEAGDA
                     QAVLLLAGDVLRSDGFRDLVDRFNVATRDHLAPIPFGGPNALFALLTRRQMGRYGLRR
                     EDYGRLVLAQRQWAAGNPNAIRRGALTIDDYLRAPLVSDPLCVLDCPPVVAGANALVL
                     "
BASE COUNT          605 a         1283 c         1210 g          568 t
ORIGIN      
        1 ctcttgccgc tcgacgcgca gccagctcgg atgacgacgg gtgcctcgct ggaaggggcg
       61 acgccgcgag agacgctcga gcgcttgctc gaggtggtcg acctagaagc gcttcgtcgc
      121 gagcagcgcg cgcgcgagcc cggcgagccg cgtctgggca tcggcctggc gacgctcgtc
      181 gagcccgcgc ccgggccacc cgacttcggc gcggcaaccg gcttccccgt tccgccggag
      241 cgggctcatg cccgggtcga gcctgacggc cggctgacgg tcttcacagc gcagcacccg
      301 cacggccaag gtcacgagac aacgctgaca caggtcgccg ccgacgagct gggcgtccct
      361 ttggatcacg tccgcgtcgt ccatggcgac acgcaggaca gcccgttcag cctgctcgga
      421 acgagtggca gccgcgccgc cacgagggcg agcggcgccg ccctctacgc tgtgcgcgac
      481 gtccggcgac aggtactcga gatcggcggt gccctgctcg agatctcgcc ggacgacctc
      541 gagatcaccg agggcgtcgt gcgcgcaaag ggcgccggga ctcgccggtt aacgctcgcc
      601 gacgtcgcac tcgccgccta cctcggctca ccggcgcttc cgccgagtca cgcaatcggc
      661 gcgctcgagg caggctggac cctcgacgac ccgccgggcg gatggtcttc cgcatcacac
      721 ctttgcgttg tcgaggtcga ccccgagacg ggaattgtcg gcatcgtccg ctacgtcgtc
      781 gtccacgact gcggcacgat gatcaatccc gcgatcgtcg aggggcaggt acggggcggc
      841 gtcgcgcagg gcatcggtgg cgcgctgctt gaggagtcgg cgtacgacga tgatggccag
      901 ttcctagctg gcaccctgat ggactaccta cttccaacag cgatggacgt gccaccgatc
      961 gagatcgttc atctcgaatc ggcgccactc gcggaggtgt cgttccgtgg tgtgggcgag
     1021 ggcggcgcga tcggggcgcc tccggcggtg acgaacgcgg tcgcggacgc tctcggcgcc
     1081 gacctcgacg aacttccgct cacccccacc cgcgtcctgc aggccctggg ggtgctccca
     1141 tgagccgccc cgtccttccc gagtggagcg gcgtcacact ccgctacctc agcccgcgcg
     1201 acacgatcaa ccggttcgga tggcagtccc cctctcaacg cgacggactc ctacccggcg
     1261 aggcgcgggc gttctcggag atcgaacaac ggctaccgcg ccaggacggg ccggtgacga
     1321 cgaccaaacg acgcttcgcg aacgcgttag aagcgacgga gacgaccaag accctcgccc
     1381 gccggctcgg cgcgcagatg gtcggcgtca cacccgtcga ccaggaatat gtctacgaag
     1441 gccggagcgt caaacaccgc tatgccgtga tcgtcgcggt cgcaatggac tacgacgaga
     1501 tcctggagct cccacgcgcc gaaacccaag aggagtacat ccgcgtctac aacgattgca
     1561 gccggatcgc agtcgaactc gccgagtgga tacggaagaa cggatacccc gcccgcgccc
     1621 acaccctgcg tgacgaagaa atcgccatgc tgccgcacgc tcaggcggcg ggactagggg
     1681 agctcggcaa gcacggatcg ctgatcaatc gtgagctcgg ctgcagcttc aggctcgccg
     1741 ccgtgacgac ggatctgccg cttgtcacag acgcgccccg cgacgaggga atcgaggact
     1801 tctgccggag ctgccgcatg tgcacggtct actgccccgg ggacgcaatc gacggagaga
     1861 aagcgctcgt acgcggcgtc gagaagtggg tcgtcgacac tgagcgatgc gccccctact
     1921 tcgggagcca ctacgcatgc ggcatctgca tccaggtgtg cccgttcaac gcgaaggcgt
     1981 tcgacggcat gttccgcgac cactacgtcg agacgatgca ggagctcgac gccgaagagc
     2041 tcgccgagca gctgacctcg acgcttgtcc cgttggcctc gccgaagctc gactaggagc
     2101 tcgcggcact ggccgcggcc ggaggctctt gctcaccgac cagctcgcgc acgagggaat
     2161 cgaaggtgtc actgaagacc gcgagatgcc ggcgcatcgc ctgctcggcg gcgtcagagt
     2221 cgtgcgcccg caacgcgtcg aggatctcgc tgtggccctc cgcctcgatc gccatcgttt
     2281 ccttcggccg tccggcacgc aggacggctg caccgtagcg ctctgtggtc tcgaacgtcg
     2341 gcagcagcaa ccgctctatc cagcgcgacc ctgatgccct cgcgagctgc gtgtggaaca
     2401 cggtgttacc ccgaacgatt gcgcggcgat cgccgcgctc gtaggcggca tcgaggtcgg
     2461 cgagcacgcc acccagggcg gcatcgtcgc cctccgtgaa gcgcaccgcc gcgcggcgca
     2521 cggccgacac ctccagtgca ctccgcacct cgtagacgtc gcgcatgtct tccccgttca
     2581 gctcagtcac gcgggccccg cgataggcga cgtgctccgc caaaccgacg gtctcgagat
     2641 agcggatcgc ctcacgaatc ggagagatgc tcatgtcgag agacgcggcg agttcttcga
     2701 ggcgaagtgg cgatcccggc ggaagatcgc cgacgatgat cgcctcccgt agcgcggcca
     2761 ccgcttcccc ggccatcgtc gcgcgcgtac gcgagcgcgg acggtcgggt gagggggaca
     2821 gaatcagtgg atcgtcggct tcgtcagctt tcatatgacc cgagctagtg ggagctacgc
     2881 ggcggttgcc tgcgtagacc tgtttggaaa cgatttgatc aaatgcttta tacagccccc
     2941 ggtgtgccag cgacgtctcg atcagcgttc aagaagcccc cgcccggatc ctctgtgacg
     3001 agaatcgaca ggtgacctcg cctgcagcca tcgccggtgt cggcgagtcg atgtacatgc
     3061 gccgccccga cggtgaccta tctacaccgg tggtgctcgc ggccgcagca cggacagcgc
     3121 tcgaggacgc gggtctcgcg ccggcagacg tggacggact cggcgtttcc tccttctcgc
     3181 ttgcccctga tcgggcgatc gacatggcgg tgaggctcgg ggttcgtgcg cgctggctca
     3241 tggacggggc gaccggaggg gcatcggcga tcgacatgct ccagcacgca cgccgagctg
     3301 tcgaggcggg cgacgcgcag gcagtgctcc tcctcgccgg cgacgttctc cgatccgacg
     3361 gcttccgcga cctggtcgac cggttcaacg tcgcgacacg tgatcacctt gccccgatcc
     3421 cattcggcgg gccgaacgct ctgttcgcgc tcctgacccg ccgccagatg ggccgctacg
     3481 gcctgcgacg cgaagactac ggccggctcg tgcttgccca gcgccagtgg gccgcgggaa
     3541 atccgaacgc catccgccga ggggcgctca cgatcgacga ctacctacgc gcgccgctcg
     3601 tcagcgatcc actgtgtgtg ctcgactgcc cgccggttgt cgcgggcgcg aacgctctcg
     3661 tcctga
//
LOCUS       DBNGNK010000273         2856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1409090,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000273 DBNGNK010000000
VERSION     DBNGNK010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1409090"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="AC150V_08035"
     CDS             <1..834
                     /locus_tag="AC150V_08035"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR3762909.1"
                     /translation="RHRGHVLCEGEQRRETIQCPYHAWTYGLDGSLRRAPRADREADF
                     DASELSLLAVAVDTWGPFVFANPNPAAAPLADTLGELPTLVAAAGVDVDALVFHRRDA
                     TDYDANWKICVENFLECYHCQIAHPGFSQVVDISPDAYVLEENRWFSSQYGPVKGAQP
                     DGFDPAGEVARGEFHLLFPGTAVNVMPGHPNVSIGPVAPLGTERTHRWLDYFFAPDTP
                     ADWIDEMLAFDAQVGHEDRALVEGVQAGVRARVVKTGVVMRSEVLIAHFQRLLADLLE
                     I"
     gene            897..>2856
                     /gene="ppk1"
                     /locus_tag="AC150V_08040"
     CDS             897..>2856
                     /gene="ppk1"
                     /locus_tag="AC150V_08040"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR3762910.1"
                     /translation="MATRTRTQEHDRLINREISWLDSSEHLLDLAADPDQPLLERVKF
                     CGIFSMIVDEFFMVRVAGLIEQAESGLGVRSADGLTPQQALAQIRERVTELTARQSKL
                     WKRELRPALAEQEIVLSSVEDCSKKELKELTRRFEREVYPVLTPLGVGPGQPFPYISG
                     LSLSLAVLAVDPATGEERFARVKVPEGFDRFLEVGARFIPVESVIAHFLPMLFPGMEI
                     SERAVFRVTRDADFELSDDADDLLGAIESELRRRRFGDVVRLELSASASAAMLNRLVT
                     GLQVEDDQVFEIEGLLDLADLTQIAGLERPELKDEPWVAVVPPRFARTTKGAIFDEIK
                     RADVLVHQPYESFRLSFEEFANAAAADPDVIGIKTTVYRTSDDSSLVSSLIDCVEQGK
                     QSVCLVELKARFDERRNIEWSRSMEQAGVHVVHGFSDLKIHAKMTLVVRREGDTLRRY
                     VHIGTGNYNASTARLYEDLGVFTADENIAGDVADLFNYLTGFGRPQRFRSLLVAPFTM
                     RARLLEEIRRVTSAAAAGEACRIRLKMNQLVDPVVIDELYAASEAGVPIKICARAICM
                     LRASVEGLSDTIEARSIFDRFLEHSRIYSFETPERSTMYISSADLMPRNLDRRIEVAM
                     PILSARVRQELNAVLDSVFADTTNAWELL"
BASE COUNT          472 a          993 c          923 g          468 t
ORIGIN      
        1 cggcacaggg gccacgtcct ctgcgagggc gagcagcgtc gcgagacgat tcagtgcccc
       61 taccacgcct ggacgtacgg cctcgacggc tctctccggc gcgcgccgcg cgccgaccga
      121 gaggcggact tcgacgcatc ggagctgtcg ctactagccg tcgcggtgga cacgtggggt
      181 cccttcgtct tcgcgaaccc caacccggcc gcggccccgc tggccgacac gctgggcgag
      241 ctgcccaccc tcgtggcggc cgccggagtc gacgtcgacg cactcgtatt ccatcgccgc
      301 gacgcgaccg actacgacgc caactggaag atctgcgtcg agaacttcct ggagtgctac
      361 cactgccaga tcgcgcatcc cggcttctcg caggtcgtcg acatctcccc ggatgcgtac
      421 gtgctcgagg agaaccgctg gttctccagc cagtacgggc cggtgaaggg cgcacaaccc
      481 gacggcttcg atccggccgg cgaggtcgcc cgcggcgagt tccacctgct cttcccgggc
      541 accgccgtca acgtgatgcc cggccatccc aatgtctcga tcggcccggt ggctccgctc
      601 ggtaccgaac gcactcaccg ctggctcgat tacttcttcg caccggacac ccccgcggac
      661 tggatcgacg agatgctcgc cttcgacgcg caggtcgggc acgaggaccg cgcactcgtc
      721 gagggggtgc aggcgggcgt ccgcgcccgt gtcgtcaaga cgggtgtcgt gatgcgctcc
      781 gaggtcctga tcgcacattt ccagcgcctc ctcgccgatc tgctggaaat ctgagcgaga
      841 cgcggagctc gagccgctac cctcaggcgg tcaaatgccg ataacagagg gctgagatgg
      901 caacgcgaac gcggacgcaa gagcacgacc ggctcatcaa ccgcgagatc tcgtggctgg
      961 actcgagcga gcacctactc gacctggccg ccgaccctga tcagccgctg ctcgagcgcg
     1021 tgaagttctg cggcatcttc tcgatgatcg tcgacgagtt cttcatggtc cgcgtcgccg
     1081 gattgatcga gcaggcggag tcggggctcg gcgtccgctc ggccgacgga ctgacgccgc
     1141 agcaagcgct ggcgcagatt cgagagcggg tcaccgagct gacggcgcgc cagtcgaagc
     1201 tgtggaagcg cgagctccgc cctgctctcg ccgagcagga gatcgtcctg agcagcgttg
     1261 aagactgctc caagaaggag ctgaaggagc tgacgcgacg gttcgagcgg gaggtctatc
     1321 ccgtcctcac accgctcggc gtggggccgg gccagccttt cccttacatc tccggcctgt
     1381 cgctgtcgct cgcggtgctc gccgtcgatc cggccaccgg cgaggaacgg ttcgcgcggg
     1441 tgaaggtgcc cgagggcttc gaccgtttcc tggaagtggg cgcacgcttc atcccggtcg
     1501 aatccgttat cgcgcacttc ctgcccatgc tcttccccgg aatggaaatc tcggaacggg
     1561 cggtcttccg cgtcacccgc gacgccgact tcgagctctc cgacgacgcg gacgacctgc
     1621 tcggggcgat cgagtctgag cttcgcaggc gccgcttcgg agacgtcgtc cgcctcgagc
     1681 tctccgcctc ggcctcggcc gcgatgctca acaggctggt gacggggctg caggtggagg
     1741 acgaccaggt cttcgagatc gagggcctgc tcgatctggc cgacctgact cagattgccg
     1801 ggctggagcg gcccgagctg aaagacgagc cgtgggtcgc ggtcgtgccg ccccgcttcg
     1861 cgcgaacgac gaagggcgcg atcttcgacg agatcaaacg cgccgacgtg ctcgtgcacc
     1921 agccgtacga gtcgttccgg ctgagcttcg aggaattcgc gaacgcggcc gcagccgatc
     1981 ccgacgtcat cgggatcaag acgaccgtct accggacgag cgacgactcg agtctcgtct
     2041 cctcgctgat cgactgcgtc gagcaaggca agcaatcggt gtgcctcgtg gagctgaaag
     2101 cgcgtttcga cgagcgccgc aacatcgagt ggtcgcggtc gatggagcag gccggagtcc
     2161 atgtcgtcca cggcttctcc gacctgaaga tccacgccaa gatgacgctc gtcgtccggc
     2221 gggagggcga cacgctgcgc cgctacgtcc acatcggaac cggcaactac aacgccagca
     2281 ccgcgcggct gtacgaggac cttggcgtct tcaccgccga cgagaacatc gcaggtgacg
     2341 tcgccgacct cttcaactac ctgaccggct tcgggcgccc gcagcggttc cgcagtctgc
     2401 tcgtggcgcc cttcacgatg cgggcccggt tgctcgagga gatccgccgc gtcacctcgg
     2461 cggccgccgc aggcgaagcg tgccgcatcc gcctgaagat gaaccagctc gtcgatcccg
     2521 tcgtcatcga cgagctctac gcggcgtccg aggcaggggt cccgatcaag atctgcgccc
     2581 gcgcgatctg catgctacgg gcgagcgtcg aggggctttc cgacacgatc gaggcccgct
     2641 cgatcttcga tcgcttcctc gagcacagcc gcatctacag cttcgagacg cccgagcgct
     2701 cgacgatgta catctccagc gcggacctca tgccgcgcaa tctcgaccgc cggatcgagg
     2761 tcgcgatgcc gatcctgagc gcacgggtcc gtcaggagct caacgctgta ctcgacagcg
     2821 tcttcgccga cacgacgaac gcgtgggagc tcctcc
//
LOCUS       DBNGNK010000274         5511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1767719,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000274 DBNGNK010000000
VERSION     DBNGNK010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1767719"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1036)
                     /gene="trxB"
                     /locus_tag="AC150V_08045"
     CDS             complement(80..1036)
                     /gene="trxB"
                     /locus_tag="AC150V_08045"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR3762911.1"
                     /translation="MSETRNVIIIGGGPAGYTAALYTARANLEPLVIEGFNWGGQLMI
                     TSDVENYPGYADGVLGPVMMQDFRRQAERFGAEFLTDDVTRVDFSERPYRVYVGDDEY
                     RAETVIVATGATARQLGLESEEKLQGRGVSYCATCDASFFREKVVVVVGGGDSAMEEA
                     SFITRFASKVHLVHRRQEFRASPIMVDRVRANEKIELVLDSVVEEVLGEDKVTGVRLR
                     NVVTNEVNDLAADGLFVAIGHDPTTALFLDSLDHDENGYLVTVGKSTATNLPGVFAAG
                     DVQDHVYRQAVTAAGTGCAAALDAERFLAEESHEAVAAKAAG"
     gene            complement(1121..2335)
                     /locus_tag="AC150V_08050"
     CDS             complement(1121..2335)
                     /locus_tag="AC150V_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR3762912.1"
                     /translation="MFAALTLVLGLAIGAIGAYLALRPALAERDRRMREAAELERALA
                     STEARLESAEHAAGERLRDAVRAASADAYRQANTAFLELAGTKLDGTVAPLRASLERV
                     EGQVKQLDQARAQSYGALREQVAVLSDRTGSLANALRSPSVRGRWGEVQLRNVVERAG
                     MLEHCDFVAQTSVRDGEGDLLRPDLVVRVPGGKSIVVDAKVPLAAYLEAFESADEAVR
                     RERLAGHARQVRDHMTKLADKRYQQQFSPAPDFVVMFVPDETLLRVAHEHDASLSEDG
                     WARGVILSSPSTLMTILRTVAAVWQQETIAESAREIHGLGSELSKRLGTFSGHLGKVG
                     RALNTAVGSYNDAVGSYETRVLVQARRLEEHGIEAEIDAPGQVERQARALSGEAPTDP
                     SVVELPRDAHAA"
     gene            2450..3073
                     /locus_tag="AC150V_08055"
     CDS             2450..3073
                     /locus_tag="AC150V_08055"
                     /inference="COORDINATES: protein motif:HMM:NF022747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HIR3762913.1"
                     /translation="MNPEKEARARRLLYFAAAAGLIGLLLAIALILFGSGSTADASRV
                     NDAMITAGCTLVSVEAQKGDHTVESRADRVDSWNTFPPTNGPHHVDPVAFGLYEDPLP
                     QAQVVHNLEHGGIAIQYGNEVPEETVDELRGFYDDHQNGTLLAPLPELGRIIAIGAWV
                     SPGGGRSGVGYLAKCAAFDEEAYATFFDEFQFKGPERFSADSLRPGQ"
     gene            3093..3181
                     /locus_tag="AC150V_08060"
     tRNA            3093..3181
                     /locus_tag="AC150V_08060"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3128..3130,aa:Leu,seq:taa)
     gene            complement(3196..4767)
                     /locus_tag="AC150V_08065"
     CDS             complement(3196..4767)
                     /locus_tag="AC150V_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762914.1"
                     /translation="MAAVSPPAADAPRRAGLVLASLILVAAVANLNLAVANVALPSIA
                     VAFDAGQVSLNLIAVGYSLGLAVSVLYLGAIGDRYGRKLMLLLGTALATPMSLLAAYA
                     PSDEVLFLARLGGGFAAGMAYPTTLALITALWAGPGRTRAIALWSGTGGAIASLGPLL
                     AGLLLEHFWWGSVFLVTLPLAGVALVMAFLLVPSHVNESTGAVDNLGGILSIVLVGTF
                     ILAINFAPVPGKGALVGGLAAVALATGVAFYLRQRRAENPLYDLDVAGRRTFWVAALA
                     GIIVFGSLMGAMFVGQQYLQNVLGYSTLEAGLAILPAALFMVAVAPRSAKLVNRFGAR
                     LTLLAGYAFCLLGFLAMLLLWKEDISYWKVGLAYALIGIGVGLAGTPASHSLTGSVPV
                     RRAGMASGTADLQRDLGGAIMQSIFGALLTAGFASAMGSAIAASGKNISESVQSQLTK
                     SFAGAEAIAKQHPQYADQITAAAETSFLQGDQWAYTAGIVAIVLGAGLVFFLFPKKEA
                     EERLLAEYHAADASA"
     gene            complement(5025..5372)
                     /locus_tag="AC150V_08070"
     CDS             complement(5025..5372)
                     /locus_tag="AC150V_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762915.1"
                     /translation="MEAVGSSSENELAARFSLDTEITGYSVEALDGPMGRVDAATYEL
                     GTRVIVVEISSPTGSRRVVLRSSAVELVDTTDEKVWVQYSRAQVESAPEHGASRIVAR
                     AAPAGDAQRGEQL"
BASE COUNT          916 a         1871 c         1881 g          843 t
ORIGIN      
        1 aggtgccggc ggaggcgtcg atatcgttgg cgtagccggt cgccacgctc gccgagcgta
       61 gcggcggctc cggactgagc tatccagccg ccttggccgc gacggcctcg tgggattcct
      121 cggcgaggaa ccgctcggcg tccagagcgg cggcgcagcc ggttcccgcg gcggtcacgg
      181 cctggcggta gacgtgatcc tgcacgtcac ctgccgcgaa gacgcccggg aggttcgtgg
      241 cggtgctctt cccgaccgtg acgaggtagc cgttctcgtc gtggtcgagc gagtcgagga
      301 acagcgcagt cgtggggtcg tggccgatgg cgacgaaaag tccgtctgcc gccagatcgt
      361 tgacctcgtt cgtgacgacg ttgcgaagcc ggacacccgt gaccttgtcc tctcccagga
      421 cttcctcgac gacgctgtcg aggacgagtt cgatcttctc gttggcacgg acgcggtcga
      481 ccatgatcgg cgaggcgcgg aactcctgac gccggtggac gaggtggacc ttgctggcaa
      541 agcgcgtgat gaagctcgcc tcctccatcg cggagtcgcc tccgccgacc accacgacga
      601 ccttttcgcg gaagaaagag gcgtcgcacg tggcacagta cgacacgccg cgcccctgca
      661 gcttctcttc ggactcgagg cccagctggc gcgcggtcgc acccgtcgcg acgatcaccg
      721 tctcggcgcg gtactcgtcg tcgcccacat agacgcggta ggggcgctcg gaaaagtcga
      781 cgcgggtcac gtcgtcggtc aggaactcgg cgccaaaacg ctccgcctgg cgccggaagt
      841 cctgcatcat cacgggcccg aggacgccgt ccgcgtaacc gggatagttc tcgacgtcac
      901 tggtgatcat cagctggccg ccccagttga acccctcgat cacgagcggc tcgagattcg
      961 cccgagccgt gtagagggcc gccgtgtagc ccgcgggccc gccgccgatg atgatcacgt
     1021 tccgtgtctc gctcactctg tcctccgaag tcaaaaggcc cctggctacg gggcttaccc
     1081 tatgagaacg aacggacgcg cggctgtgtt ccttgcgcga ctacgcggcg tgcgcgtcgc
     1141 gggggagctc gacgaccgac ggatcggtcg gcgcttcgcc agagagcgcg cgcgcctgcc
     1201 gctcgacctg gcccggcgcg tcgatctcgg cttcgatgcc gtgctcttcc aggcggcgcg
     1261 cctgcaccag cacgcgcgtc tcgtaggagc cgactgcatc gttgtacgag ccgacggcgg
     1321 tgttgagtgc gcggccgacc ttgccgaggt ggcccgagaa cgtgccgagg cgcttcgaga
     1381 gctcactgcc cagcccgtgg atctcgcggg cgctctcggc gatcgtctct tgctgccaga
     1441 cggctgccac ggtccggagg atcgtcatca gcgtcgaggg ggaggagagg atgaccccgc
     1501 gcgcccagcc gtcctcggac aggcttgcgt cgtgctcgtg ggccacccgc agcagcgtct
     1561 cgtcggggac gaacatgacg acgaagtcgg gcgccggcga gaactgctgc tggtagcgct
     1621 tgtcggcgag cttggtcatg tggtcgcgca cctgccgggc atgcccggcg aggcgctctc
     1681 gccgcacggc ctcgtcggcg gactcgaacg cctcgaggta ggcggcaagc ggcacctttg
     1741 catcgacgac gatcgacttg ccaccgggca cccgcacgac cagatcggga cggagaaggt
     1801 caccctcgcc gtcgcgcacg gatgtctgag cgacgaagtc gcagtgctcg agcatgcccg
     1861 cccgctcgac gacgttgcgt agctgcacct cgccccagcg tccacgcacg gagggcgagc
     1921 gaagcgcgtt ggcgaggctg ccggtgcggt cgctcaggac cgcaacctgt tcccgcagcg
     1981 ctccgtacga ctgagcccgc gcttggtcga gctgcttgac ctggccctcg acgcgctcga
     2041 gcgatgcccg cagtggggca acggtgccgt cgagcttcgt tccggcgagc tcgaggaatg
     2101 cggtgttcgc ctggcggtag gcgtcggctg aggccgcgcg aacggcgtcg cgcagccgtt
     2161 ctcctgccgc atgctcggca gactcgagcc gcgcctcggt gctcgcgagc gcacgctcca
     2221 gctcggccgc ttcgcgcatc cgccgatcgc gctccgccag ggccgggcgc agcgccaggt
     2281 aggcaccgat tgcgccgatc gcgagcccga gcaccaatgt cagtgcggcg aacatggggc
     2341 aagaaagagt aggcgaggcg tcggacggat cccgctcagc aggccgctgc tagcgtgttt
     2401 tgcagtgcct ccgcggacgc gcaatcccgt gccgagagga cgggcgccga tgaaccccga
     2461 gaaggaggct cgggctcgtc gactgctcta cttcgctgcg gcggccgggc tgatcgggct
     2521 gttgctcgcg atcgcgctga ttctcttcgg gagtggctcc acggccgacg cttcccgcgt
     2581 gaacgacgcg atgatcaccg ccggatgcac gctcgtctcg gtcgaggcgc agaagggcga
     2641 ccacacggtc gagtcccgag cagaccgggt cgactcgtgg aacaccttcc cgccgacgaa
     2701 cgggccgcac catgtcgatc cggtcgcgtt cgggctctac gaggatccgc tgccgcaagc
     2761 tcaggtggtg cacaacctcg agcacggggg gatcgcgatc cagtacggga acgaggtgcc
     2821 cgaggagacc gtcgacgagc tgcgtggttt ctacgacgac caccagaacg gcacgctgct
     2881 cgctccgctg cccgagctcg gccgcataat cgcgatcggc gcctgggttt cgccgggggg
     2941 aggccgatcg ggcgtcggct acctcgccaa gtgcgccgcc ttcgacgagg aggcctatgc
     3001 gaccttcttc gacgagttcc agttcaaggg ccctgagcgc ttcagcgcag atagcctcag
     3061 gccaggccag taggcgtcta cactcggctc acgccggggt ggcggaattg gtcagacgct
     3121 cccgccttaa aagcgggtgt tcccttctgg ggacgtgcgg gttcgactcc cgcccccggc
     3181 actcgtggcg tctcactacg cggacgcgtc ggccgcgtga tactcggcga gaagtcgctc
     3241 ctcggcctcc ttcttcggga agaggaagaa gacgagaccg gcgccgagca cgatcgcgac
     3301 gatgccggcg gtgtaggccc actggtcgcc ttgcaggaac gaggtctcgg ctgcggcggt
     3361 gatctgatcg gcgtactggg ggtgttgttt cgcgatagcc tcggcgcccg cgaacgactt
     3421 ggtcagttgg ctctgaacgc tttcgctgat gttcttgccg gacgcggcga ttgctgatcc
     3481 catcgccgag gcgaatccgg ccgtcagcag ggccccgaag atcgactgca tgatcgctcc
     3541 gcccaggtcg cgctgcaggt cggcggtgcc cgaggccatc ccggcacgcc tgacgggcac
     3601 cgagccggtg agggaatggg aggcaggcgt tccggcaaga ccgacgccga tcccgatcag
     3661 ggcgtaggcc agcccgactt tccagtagga gatgtcctcc ttccagagca ggagcatggc
     3721 cagaaagccg agcaggcaga aggcatagcc tgcgaggagc gtcagccgag ccccgaacct
     3781 gttcacgagc ttggccgacc ggggtgcgac ggcgaccatg aacagcgcag ccgggaggat
     3841 ggcaagcccc gcctcgagcg tcgagtagcc gaggacgttc tgcaggtatt gctgaccgac
     3901 gaacatcgcg cccatcaggg agccgaagac gatgatcccc gccagggcgg cgacccagaa
     3961 cgtacgccga ccggccacgt cgaggtcgta gagcgggttc tccgcgcgac gctgccgcaa
     4021 gtagaaggcg actccggtcg cgagcgcgac cgctgccaag ccgccgacga gtgccccctt
     4081 gccggggacg ggcgcgaagt tgatcgcgag gatgaacgtc cccacgagga cgatcgagag
     4141 gatcccaccg aggttgtcca ccgcgcccgt ggactcgttg acatggctgg gcacgagcag
     4201 gaacgccatg acgagggcga ccccggccag aggcagtgtg accaggaaga ccgagcccca
     4261 ccagaagtgc tccagaagca acccggcaag cagcgggccc agcgacgcaa tcgcacctcc
     4321 ggtccccgac cagagcgcga tcgccctggt acggcccggc cccgcccata gggcggtgat
     4381 cagcgccagc gtggtcgggt aggccatccc ggctgcgaag ccgccaccca ggcgtgcgag
     4441 aaacagcact tcgtcggaag gagcgtaggc ggccaagagc gacatcggag ttgcgagtgc
     4501 cgtccccagc aacagcatca gcttgcggcc gtagcggtcg ccgatcgccc cgaggtacag
     4561 aaccgagacg gccaggccga gcgaatagcc gaccgcgatc agattcagcg acacctggcc
     4621 cgcgtcgaac gcaaccgcga tcgacggcaa tgcgacgttc gccaccgcca ggttcagatt
     4681 cgctaccgcg gcgaccagga ttagcgaagc gagaacgagt ccggcgcggc gcggcgcatc
     4741 cgccgctggt gggctcaccg ccgcaatctt cctgagcttt cacaggaatg ggacatcacg
     4801 tcgtcctcag tgttcgccgc gctccccggt tgtgacggcg caacacgggg gggcacacta
     4861 gcgaatgagg ccgaccgaga tggttcgatc gaccggggag cacagagctc gcgtgaggcc
     4921 gatttcccct gactccgccc agactggccg agacgcctgg cgcgacgtac gagcgggtgt
     4981 ccccatctgg gaacgtgcgg gttcgactcc cgcccgcggc atcgtcaaag ctgctcgccg
     5041 cgttgagcgt cgcccgccgg agcggcccgt gcgacgatcc gtgaggcgcc gtgctccgga
     5101 gcggactcga cctgggctcg gctgtactgc acccagacct tctcgtccgt cgtgtcgacc
     5161 agttcgaccg cgctcgagcg gaggaccact cgcctgctgc ccgtcggcga cgagatctcg
     5221 acgacgatga cccgcgtgcc cagctcatac gtcgccgcgt cgacccgtcc catcggcccg
     5281 tcgagtgcct cgacgctgta gccggttatc tcggtgtcca gcgagaaacg tgcggcgagc
     5341 tcgttctcag acgacgagcc aactgcttcc atcacacgac ctcccacgac gtgccgcaga
     5401 aatgacgtgc cgcagagtta cccgtacggg aatatgacaa actcgcgcgc cctcggaggc
     5461 ccctgagcag agcacgcccg acgcggtgtc cgcgcaggtt tcacctccgg a
//
LOCUS       DBNGNK010000275         3114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_549281,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000275 DBNGNK010000000
VERSION     DBNGNK010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_549281"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="AC150V_08075"
     CDS             <1..897
                     /locus_tag="AC150V_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR3762916.1"
                     /translation="FALGPLCGVDDPDAVLAAGARCDELGLDTISAGGTIAWAMECAE
                     RGLIDAPWLRFGDASSLLRALDEIGARNGLGDLLAEGSRRAAEHVGGDSLELAPQVKG
                     LELPGYDPRTLQTMALGLAVNPRGADHNRSGAYEADLSGRVDRLALGEEHVQAAIETE
                     DRAAVMDSLILCKFLRGVFEDPFAEWAGLLRPVTGWDVDEDELRETARRIVVTKRIYN
                     LEEGWTRSEDWLPARLLEEPVSVGEREGAIDPARLRSMIDRYYAERGLEPSGRPRDET
                     LTALGIEPRPSARATAPPQPVG"
     gene            905..>3114
                     /locus_tag="AC150V_08080"
     CDS             905..>3114
                     /locus_tag="AC150V_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR3762917.1"
                     /translation="MAVSAPPDTVTLSIDGTELTVPGGTTIWTAARDVGIEIPVLCHD
                     ERYDPVGVCRMCCVDAGGRVLAAACVRACEDGMTVRTDTAEVLRSRETLTRLLTADQP
                     DWAEDPKQTTTADNELTKLALGYDLRRDSAGLPTGGDERPRDLSNPVIAVDHPACILC
                     DRCVRACDDVQGNDVIGRTGKGYATRIAFDLDDPMGESSCVTCGECVAACPTGALTNK
                     PITLPLRPRSELKQVDSVCPYCGVGCALTYHVDPEENTIVFADGRDQPGSQQRLCVKG
                     RYGWDYALNPQRLTAPLIRRGDRYPKGPLSADVRGEGDGRRKPGGLVDYGEVLPAFRE
                     ASWEEALDLVARRLTEIHAAAGPGSIAGFGSAKCSNEEAYLFQKLIRAGFGTNNVDHC
                     TRLCHASSVAALLEGVGSGAVSTTYGDIVNAEVALLAGTNTTANHPVASSFFKQARRR
                     GTTLIVVDPRRERIADHADIFCQLKPGTDVAFYNGVMHEVIRLGLVDRTFVEQRTDGF
                     AELETLVAEYPPERASKICGVDPDTIREVARLWGEAGTGVVYWGMGISQHTTGTDNAR
                     CLIAMCCITGNVGRAGTGLHPLRGQNNVQGASDAGLIPMMYPDYQLVTDPSVQALFEE
                     AWGRSLSGETGLTVTEIIASALQGGVRGMYMMGENPFLSDPNVNKVRKALAALEFLVV
                     QDIFLTETAEFADVVLPASSYLEKDGTYTNTDRRVQRGHKALEPPGQAREDWKIV"
BASE COUNT          510 a         1103 c         1067 g          434 t
ORIGIN      
        1 ttcgcgctcg ggccgctctg cggagtggac gatccagacg ccgtgctcgc cgcgggcgcc
       61 cgttgcgacg agctcggact cgacacgatc tccgccggcg gcacgatcgc atgggcgatg
      121 gagtgcgccg aacgcggcct gatcgacgct ccgtggctcc gtttcgggga cgcctcgtcg
      181 cttctgagag ccctcgacga gatcggcgcc cgcaacggcc tcggcgactt gctcgcggag
      241 ggctcccgcc gggccgccga gcacgtcggc ggcgacagcc tggagctcgc gcctcaggta
      301 aagggcctcg agctcccggg ctacgacccg aggacactcc agacgatggc gctgggcctg
      361 gcggtgaacc cgcgcggcgc cgaccacaac cgatcgggag cgtacgaggc cgacctgtcc
      421 ggtcgcgtcg atcggctggc actcggcgag gagcacgtac aggcggcgat cgagaccgag
      481 gaccgagcag cggtgatgga ctcgctcatc ctctgcaagt tcctgcgtgg cgtcttcgaa
      541 gaccccttcg ccgagtgggc ggggctgctc cggcccgtca ccggttggga cgtcgacgag
      601 gacgagctgc gcgagaccgc ccggcgcatc gtcgtcacga agcggatcta caacctcgaa
      661 gagggttgga cgcggtccga ggactggctc cccgcccgcc tcctggagga gcccgtctcg
      721 gtcggcgagc gcgagggagc gatcgacccg gcacgcctgc gctcgatgat cgaccgctac
      781 tacgccgaac ggggcctgga accgtcgggc agaccgcggg acgagacgct caccgcactg
      841 ggcatagagc cgcgccccag cgctcgtgcg acggcccctc cccagcctgt cggataatgg
      901 cgtgatggcc gtctcggcgc cccctgacac cgtgacgctg tcgatcgacg gcaccgagct
      961 gaccgtgccc gggggcacga cgatctggac ggccgcgcgc gacgtgggca tcgagatccc
     1021 cgtgctgtgc cacgacgagc gctacgaccc ggtcggagtc tgccgcatgt gctgcgtcga
     1081 cgccggcggc cgcgtcctcg ccgccgcctg cgtgcgcgcc tgcgaagacg gcatgaccgt
     1141 ccggaccgac acagccgagg tgctccgctc acgcgagacg ctgacacgcc tcctcacggc
     1201 cgatcaaccc gattgggcgg aggatccgaa gcagaccacg accgccgaca acgagctcac
     1261 gaagctggcg ctcggatacg acctccggcg cgacagcgcc gggctgccga ccggcggcga
     1321 cgagcgcccg cgcgacctct cgaatccggt catcgccgtc gaccaccctg cctgcatcct
     1381 ctgcgaccgc tgcgtccgcg cctgtgacga cgtccaggga aacgacgtga tcgggcggac
     1441 ggggaaggga tacgcgaccc gcatcgcctt cgatctcgac gatcccatgg gtgagtccag
     1501 ctgcgtcacg tgcggcgagt gcgtcgccgc ctgcccgacc ggcgcgctga ccaacaagcc
     1561 gatcaccctg ccactgcgcc cgcgcagcga gctcaagcag gtggacagcg tctgccccta
     1621 ctgcggcgtc ggctgcgcgc tcacgtacca cgtcgacccg gaagagaaca cgatcgtgtt
     1681 cgcagacgga cgcgaccagc ccggctcgca gcagcgcctc tgcgtcaagg gtcggtacgg
     1741 ctgggactac gcgctgaacc cacaacggct gacggcgccc ttgatccgtc gcggcgaccg
     1801 atatccgaag ggcccactct cggcagacgt ccgcggcgag ggcgacggca ggaggaagcc
     1861 gggcggcctg gtggactacg gtgaggtgct gccggccttc cgcgaggcga gctgggagga
     1921 ggcactcgac ctggtcgcgc ggcggctgac cgagatccac gccgccgccg ggcccgggtc
     1981 gatcgcgggc ttcggctcgg cgaagtgctc gaacgaggag gcctacctct tccagaagct
     2041 gatccgggca ggcttcggca ccaacaacgt cgaccattgc acacgcctct gccacgcctc
     2101 cagcgtcgcc gcgctgctcg agggcgtcgg ctccggcgcc gtgtccacga cctacgggga
     2161 catcgtcaat gccgaggtcg cgctcctcgc cggcacgaac acgacggcca atcaccctgt
     2221 cgcctcctcg ttcttcaaac aggcacgccg gcgcggcacg acgctgatcg tggtcgaccc
     2281 gcggcgggag cgaatcgccg accacgcgga catcttctgc cagctgaagc ccggcaccga
     2341 cgtcgccttc tacaacggcg tcatgcacga ggtaattcgc ctcgggctcg tcgaccgcac
     2401 cttcgtcgag caaaggacag acggcttcgc agagctcgag acgctggtcg ccgagtaccc
     2461 gccggagcgg gcctcgaaga tctgcggggt cgacccggac acgatccgcg aggtcgcccg
     2521 gctctggggt gaggccggta ccggagtcgt ctattgggga atggggatct ctcaacacac
     2581 gaccgggacg gacaacgcgc gctgcctgat cgcgatgtgc tgcatcaccg gaaacgtcgg
     2641 ccgagcaggc accgggctcc atccgctgcg cggccagaac aacgtccagg gggcttccga
     2701 tgcgggcctc atcccgatga tgtacccgga ctaccagctg gtgaccgacc cgagcgtgca
     2761 ggctttgttc gaggaggcgt ggggtcgctc tctctccggc gagacggggc tgacggtgac
     2821 cgagatcatc gcgtccgcgc tccagggagg cgtgcgtggc atgtacatga tgggcgagaa
     2881 tccgttcctc tccgacccga acgtgaacaa ggtgcgcaag gcgctcgccg cgctcgagtt
     2941 cctcgtcgtc caggacatct tcctgacgga gacggccgag ttcgcggacg tcgtgctgcc
     3001 ggcgtcctcg tatctcgaga aggacggcac ctacacgaac accgaccgac gcgtgcagcg
     3061 tggccacaag gcgctcgagc cgcccgggca ggcacgcgag gactggaaga tcgt
//
LOCUS       DBNGNK010000276         5678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_334003,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000276 DBNGNK010000000
VERSION     DBNGNK010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5678
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_334003"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="AC150V_08085"
     CDS             <1..398
                     /locus_tag="AC150V_08085"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR3762918.1"
                     /translation="YARRDPDFHRERRAAVTAFTGALPLFLLLPTAPPRVLDGFVDTL
                     AQGGVDIEHPLLVRFYNPIAAMPSHHVAFAVVTGTGLAQRSQGGLQQAAWCAYAPLVA
                     TIVVATGNHFVLDIAAGALLGAAARRVTR"
     gene            395..1474
                     /locus_tag="AC150V_08090"
     CDS             395..1474
                     /locus_tag="AC150V_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR3762919.1"
                     /translation="MSSTGRRDEQVTAESGERAPRFAWKRVILGGALAFVVGSGVVAL
                     IGNAAGFSNLFDTLQEGDPVWLVACAVGQLVVFGGYATVFRGTARFEGGPRVPLNLSL
                     RVVLGSFALTQLIAFGGAAGLAVMFWAMRKLGFETRESAVRVIGLNAFVYLVFAALGW
                     LTALIALVFGLAPSGLTLPWLAIVPILFGLAYWFTDPSRVVSWASPAGNWLRRALAIG
                     VSAAWWVRRVVLSAQGRTMGVAAGVYWLGDIASLWAGLMVFDAAPGIIPLALAYFTGY
                     VASILPLPFIATGGVDAATTFALTAVGVPLEGALLGVVAHRLFAFWLPVIPGLVLAAL
                     LPRTGRALERAAATRGQDVEPATAT"
     gene            complement(1483..2619)
                     /locus_tag="AC150V_08095"
     CDS             complement(1483..2619)
                     /locus_tag="AC150V_08095"
                     /inference="COORDINATES: protein motif:HMM:NF016218.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HIR3762920.1"
                     /translation="MAVDTKRRAQANEPAGRRAARRARSEDGRRTFPAGHALIVAALA
                     LLLGVLLNAQGIYKTAYNQPEGAKREIALAFANPLRHASHALGLDRPREWLRSAIGRG
                     GEDEIDTATELPPAQPSAPRAARPPAATPPPAATPAPVPPPAAPAKVAFTPKDPLRLW
                     VAGDSLAIIPGWAVIRAAGTKGVIEALEGVDGRVATGLERPDVFNWFTHVTERVKTLK
                     PDAVVLGFGGNDDHGYMTGLPEGVTIDRFGGPAWVKEYRRRVGGLMDTVIRGGGVVVW
                     IGLPITRSETQTQRFDVINAAVAAEARERPKKVAFVDTYTMFASDSGGYTEYLEDTAG
                     RLQKVRAGDGVHFDRAGGDIIAREVLTRLNQFYDLTSWRKKKSG"
     gene            complement(2619..4037)
                     /locus_tag="AC150V_08100"
     CDS             complement(2619..4037)
                     /locus_tag="AC150V_08100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR3762921.1"
                     /translation="MLFPTATFAIFFMVVLPLSWLLMPHVHRWRPFIIVASFVFYSWW
                     DWRFVLLLAGSILWNQLIAVRIHRASVQSQRKALLAVALAGNIGVLAYFKYYDFFVSS
                     TQNLATIVGLDLPLSVRDVVLPVGISFYTFMAISYVVDTYRGDTVPTTLEKFAAYLSF
                     FPHLVAGPIVRPNELIPQMETPRDPRVVDTSRAFYLIATGLFKKVVIANYLAANIVDE
                     VFATPEQHSSLEILIGIYAYAVQIYADFSGYTDIAIGIALLLGFKFPQNFDSPYAAVS
                     IQDFWRRWHMTLSRWLRDYVYIPLGGNRKGIPITYRNLMLTMLIGGLWHGAGWTFIVW
                     GGIHGLALCAERWWRTRAGYVVPRDTFLRRNWQRIVTFHVVCFAWIFFRSDSFSTAWE
                     MITGLVTGWGEASPLVTAGVLLAIAVGIASQYLPASVPRIVMAQFGRLPVAGQAVVLA
                     FGLMVIQAMGPEGVAPFIYFQF"
     gene            4121..4693
                     /locus_tag="AC150V_08105"
     CDS             4121..4693
                     /locus_tag="AC150V_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3762922.1"
                     /translation="MARLVLASLAASLALAAVVQAGAAGAPRTVYHVGDSLAYGTSLH
                     LQSFLKGWKIQKTVRVSMQVYDVPGLVRDKGRSLPAVVIVSAGTNGHPSSFALFAANV
                     RATVRAVGSGRCLIWANIVRPPYQGVTYERMSRALQTLADRYRNLVVFDWAALVGRNP
                     EWLASDGVHVTSAGYRARARALAQAARRCP"
     gene            4840..>5678
                     /locus_tag="AC150V_08110"
     CDS             4840..>5678
                     /locus_tag="AC150V_08110"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR3762923.1"
                     /translation="MKVWFLKGEQMYAVERPGSSAEAAIRQLLEGPTAAEGQEGIRTY
                     IPGGTPLRDVSVANGTVTVDLGEKFVQGRAADTLLARLSQVVHTLTGSEGGRRVKLLI
                     KGGTPLGVFPGIATAAPVTVAYLEAPNVPAPRKPVPSGIPVKESVRAMQERLVELGFL
                     LPGDVDGQNGPATQTAVLAFQKWEGLERDGVAGPQTLQRMETAERPTPITKQGRGKRA
                     EVLLEVAQVLGDGDPHPRAALLEGGRAQQEGLVQRAAEVYEHRLAGLDVDRLELSIPQ
                     RARP"
BASE COUNT          847 a         1931 c         1962 g          938 t
ORIGIN      
        1 tctacgcacg gcgtgacccc gatttccatc gcgagcgacg ggccgctgtc accgccttca
       61 cgggtgcatt gccactcttc ctgctgctgc cgacggcacc accgcgcgtc ctcgacggct
      121 tcgtggacac gctcgcgcag ggaggcgtcg acatcgagca cccgttgctg gtgcgcttct
      181 acaatccaat tgcggcgatg ccgagccacc atgtcgcgtt cgccgtcgtg accggtaccg
      241 gcctcgccca acggtcgcag gggggccttc aacaagccgc ctggtgcgcg tacgctccgc
      301 tcgtggcgac catcgtggtg gcaacgggaa accacttcgt cctcgacatc gctgcgggtg
      361 cgctgctcgg cgccgccgcg cggagggtta ccaggtgagc tcgaccggcc ggcgtgacga
      421 gcaggtgacg gcggagagcg gcgagcgtgc gccccgcttt gcctggaagc gggtcattct
      481 cggcggcgcg ctcgcattcg tcgtcgggag tggcgttgtc gcgctcatcg gcaacgcggc
      541 ggggttctcg aatctgttcg acacgttgca ggaaggcgac cccgtatggc tcgtcgcctg
      601 cgccgtcggt caactcgtcg tattcggggg ctacgcgacc gtcttccgcg gcacggctcg
      661 gttcgaaggc ggcccgcgag tgccgctgaa cctctcgctg cgcgtcgttc tcggcagctt
      721 cgcactcacc cagctgatcg cgttcggcgg ggccgctggg ctcgcagtga tgttctgggc
      781 gatgcgaaag ctcgggttcg agactcgtga gtcggctgtc cgcgtcatcg gcctcaacgc
      841 tttcgtctac ctcgtcttcg ccgcgctcgg gtggttgacg gcactcatcg cgctcgtctt
      901 cggcctcgct ccgtcgggac tgacgctgcc gtggctcgcc atcgtcccga ttctgttcgg
      961 gctcgcctac tggttcacgg atccgagccg tgtcgtgagc tgggcatcgc ccgccgggaa
     1021 ctggcttcgt cgcgctctgg cgatcggcgt ctcggccgcc tggtgggtgc ggcgggtcgt
     1081 cctctcggcg cagggccgga cgatgggggt cgccgccggc gtgtactggc tgggtgacat
     1141 cgcgagcctg tgggcgggtc tgatggtctt cgacgcggcc ccgggcatca tcccgctcgc
     1201 gctcgcatac ttcacgggct acgtcgcctc gatcctcccg ctgccgttca tcgcgacagg
     1261 cggcgtcgat gcggcgacga cgttcgcgtt gaccgcggtt ggagtgccgc tcgagggcgc
     1321 gttgctcgga gtggtagccc atcgcctgtt cgccttctgg cttcctgtga tccccgggct
     1381 ggtgctggcc gctctcctcc cgagaacggg tcgcgccctg gagcgggccg ctgccacacg
     1441 cgggcaggac gtcgagccgg ccacggccac gtagagaggc cctcagccgc tcttcttctt
     1501 gcgccagctc gtgaggtcgt agaactggtt caggcgcgtg agcacctcgc gcgcgatgat
     1561 gtcgccgccc gctcggtcga agtgcacgcc gtcgcctgcg cggaccttct ggagtcggcc
     1621 ggccgtgtcc tcgaggtact ccgtgtagcc gcccgagtcc gaggcgaaca tcgtgtacgt
     1681 gtcgacgaat gcgactttct tcggccgctc gcgcgcctca gcggcgaccg ccgcgttgat
     1741 cacgtcgaac cgctgtgtct gcgtttcgct cctcgtgatg ggcagcccga tccagacgac
     1801 gacgccgccg ccgcggatta cggtgtccat caggccgccg actcggcggc ggtactcctt
     1861 gacccacgcc ggaccgccga accggtcgat cgtcactccc tccggaagcc cggtcatgta
     1921 gccgtgatcg tcgttgcccc cgaaaccgag gacgacggcg tcgggcttga gcgtcttgac
     1981 tcgctcggtg acgtgggtga accagttgaa gacgtcaggt cgctcgaggc ccgtagccac
     2041 ccggccgtcg acgccctcga gcgcctcgat cacgcccttc gttcctgcgg cgcggatgac
     2101 ggcccacccg gggatgatcg cgagtgagtc gccggcaacc cacagcctga ggggatcctt
     2161 tggggtgaag gcgaccttgg ccggcgccgc gggaggcgga accggggcgg gcgtcgccgc
     2221 cggtggcgga gtggcggccg ggggcctggc ggcccgcggt gcgctcggct gagccggggg
     2281 cagctcggtc gccgtgtcga tctcgtcctc gccgccgcgc ccgatcgcgc tgcgcagcca
     2341 ctcgcgcggg cggtcgagcc cgagcgcgtg gctcgcatgg cgaagcgggt tcgcgaaggc
     2401 aagggcgatc tcacgtttcg cgccctccgg ctggttgtag gcggtcttgt agatcccctg
     2461 cgcgttgagc agaacgccga gcaggagtgc cagcgcggcc acgatcagcg cgtgcccggc
     2521 cgggaacgtt cggcgcccgt cctcgctccg cgctcgtcgc gcagcccgcc gccccgccgg
     2581 ctcgttcgcc tgcgctcttc gcttcgtatc gactgccatc agaactggaa gtagatgaag
     2641 ggtgccacgc cctccggccc catggcctgg atcaccatca acccaaaggc gaggacaacc
     2701 gcctggcccg caaccggcag ccgtccgaac tgcgccatga cgatccgggg cacgctggcc
     2761 ggtaggtact gcgaagcgat tccgacggcg atcgcaagga gcacgccggc cgtcaccagc
     2821 ggtgacgcct caccccagcc cgtcacgagc ccggtgatca tttcccacgc cgtcgagaac
     2881 gagtccgagc ggaagaagat ccaggcaaag cagacgacat ggaaggtgac gatgcgctgc
     2941 cagttgcggc gcaggaacgt gtcccgcggc acgacataac cggcgcgcgt acgccaccac
     3001 cgctcggcac agagcgccag tccgtggatc ccgccccaca cgatgaaggt ccagccggcc
     3061 ccgtgccaca ggcccccgat cagcatcgtc agcatcaggt tgcggtaggt gatcggaatg
     3121 cccttccggt tgcctccgag cgggatgtag acgtagtcgc gcagccagcg cgagagcgtc
     3181 atgtgccagc gacgccagaa gtcctgaatc gacacggccg cgtacggaga gtcgaagttc
     3241 tgcgggaact tgaacccgag cagcagcgcg atcccgattg cgatgtccgt gtagccggag
     3301 aagtcggcgt agatctgcac cgcgtacgcg tagatgccga tcaggatctc gagcgacgaa
     3361 tgctgctccg gggtcgcgaa gacctcgtcg acgatgttgg ctgccaggta gttggcgatc
     3421 accaccttct tgaacagccc ggtggcaatg aggtagaagg cgcggctcgt gtcgacgaca
     3481 cgagggtcgc gcggggtctc catctgtggg atcagctcgt tcgggcgaac gatcgggccg
     3541 gcgacgaggt gcgggaagaa cgacaggtaa gcggcgaact tctcgagcgt cgtcggcacg
     3601 gtgtcgccgc ggtaggtgtc gacgacgtag ctgatcgcca tgaacgtgta gaaagagatg
     3661 ccgaccggga gaacgacgtc gcggaccgag agcggcagat cgagccccac gatcgtggcg
     3721 aggttctgcg tcgagctgac gaagaagtcg tagtacttga agtaggcgag gacgccgata
     3781 ttcccggcca gcgcgaccgc gagcagcgcc ttgcgctggg actgcaccga cgcacggtgg
     3841 atccgcaccg cgatcagctg gttccagagg atcgagccgg ccagcagcag cacgaagcgc
     3901 cagtcccacc agctgtagaa gacgaagctg gcgacgatga tgaacggccg ccaccggtgg
     3961 acgtgcggca tcagcagcca cgacagcggc agcacgacca tgaagaagat cgcgaaagtt
     4021 gcagtcggga agagcacggc ggcagcctag atagcttgct ggataggtcc ggcgcagcga
     4081 gggagggatc tcctcgctcc gggcgtaccc ttctcccgaa gtggcccgac tcgtccttgc
     4141 gtcactcgcc gcctcactcg cactcgccgc cgtggttcaa gccggggcgg ccggagcccc
     4201 tcggacggtg taccacgtcg gcgactcgct cgcctacggc acgtctctgc acctccagtc
     4261 cttcctgaag ggttggaaga tccagaagac ggtccgcgtc agcatgcagg tgtacgacgt
     4321 tccgggcctc gtgcgcgaca agggtcggtc gctccccgca gtcgtcatcg tcagcgccgg
     4381 gaccaacggt catccgagct ccttcgctct ctttgccgcc aacgtgcgcg cgacggttcg
     4441 cgccgtaggc tccggccggt gcctgatctg ggcgaacatc gtccggcccc cctaccaagg
     4501 ggtgacttac gagcgtatga gccgcgcctt gcagacgctc gccgaccgct atcgcaatct
     4561 cgtcgtcttc gactgggcgg cgctggtcgg ccgcaatccc gagtggctcg cttcggacgg
     4621 cgtgcacgtc acgagcgcgg gctaccgggc gcgtgcccgg gcgcttgctc aggctgcgcg
     4681 gcgttgccct tgagcggacg gtcccgttag cctctcgggt ctcgtcgtgg gtctcatcgc
     4741 ccctcccgcc cgccggcgtc tgctcgccct cctggtcggc gtcatcgccg cactgctcgg
     4801 cctcccggcg ctcgtgacgg ccggctcgac gcagcgacag tgaaggtctg gttcctcaag
     4861 ggtgagcaga tgtacgccgt cgagcggccc ggctcgagcg cggaagccgc gatccggcag
     4921 ctgctcgagg ggccgacggc ggccgagggg caggagggga tccgcaccta catcccgggg
     4981 ggcacgccgc tccgggacgt ctctgtcgcg aacggcactg tgacggtcga cctcggcgag
     5041 aagttcgtgc agggccgcgc ggccgacacg ctgctcgccc gcctgtctca ggtcgtacac
     5101 acgctgaccg gctccgaggg tggacgacgc gtgaagctgt tgatcaaggg cggtacgccc
     5161 ctcggagtgt ttccaggtat cgcgacggcg gcgccggtca ccgtcgctta cctggaggcg
     5221 cccaacgtcc cggctccgcg caaacctgtt ccgagcggca tcccggtgaa ggaaagcgtc
     5281 cgtgccatgc aggagcgact cgtcgagctc ggctttctcc tgccaggcga cgtggacggc
     5341 cagaacggtc ctgcgacgca gaccgcggtg ctcgccttcc agaagtggga ggggctcgaa
     5401 cgcgacggtg ttgcgggtcc gcagacgctg cagcgcatgg agacagcgga acgtccgacg
     5461 ccgatcacga agcaaggacg ggggaagcgc gccgaggtgc tcctcgaggt cgctcaggtc
     5521 ctcggcgacg gcgatccgca cccgcgcgcc gcccttctcg aaggcggccg tgcgcagcag
     5581 gaggggctgg tccagcgggc cgcggaggta tacgagcacc gcctggccgg gctcgacgtc
     5641 gaccggctcg agctctcgat cccacagcga gcgcgacc
//
LOCUS       DBNGNK010000277         2679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1982862,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000277 DBNGNK010000000
VERSION     DBNGNK010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1982862"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2679
                     /gene="rpoB"
                     /locus_tag="AC150V_08115"
     CDS             <1..>2679
                     /gene="rpoB"
                     /locus_tag="AC150V_08115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3762924.1"
                     /translation="NQRLGVGVPESTRVLTTEDILALVRKLVELPDTLGVPEESKDYA
                     ADAAGLPRDPIRGDLDEYEHFGNRRLRTTGELIQEAFRVGLYRMERVVRERMTTEDVD
                     TITPQTIINIRPVVAALKEFFGSSQLSQFMDQTNSLAGLTHRRRLSALGAGGLTRERA
                     PIEVRDVHPTHYGRMCPIETPEGPNIGLIGSLASYATVSEFGFIQTPYRVVKNGKVGD
                     EIVYLDAAEEAQFTIAQASEPIDPKTGKFLHDQVLCRSREGEAVVADPKSIEYMDVSP
                     AQIVSVATALIPFLEHNDANRALMGANMQRQAVPLMIPQAPLVGTGLEFRAAVDTGDV
                     VLAAADGSVIEVDAEHIVIEGRGSRNEYPLTKFMRSNQGTLIHQKPAVKLGDRVKKGS
                     VLADGSSTDGGELALGANLLVAFMPWEGFNFEDAIVISERLVRDDVLSSIHIHEYEID
                     ARSTKLGDEEITRDIPNRSEESLANLDERGVVRIGAEVTSGDLLVGKVTPKGETELTA
                     EEKLIRAIFKEKAREVRDTSLKVPHGEGGKVIGVKTFSRDLGDDLPPGVNELVRVYVA
                     KKRKIAEGDKLAGRHGNKGVIARIVPEEDMPFLEDGRSVDVVLNPLGVPSRMNIGQIL
                     ETHLGWAAAHGVFADENGVRVNDRAPINAPDPHPVATAVFDGATEQDVDDALVAWVKS
                     NPDFPVKMNVDEKRTEGRKCSGKVRLYDGKTGQQFEQKVTVGYMYILKLLHLVDDKIH
                     ARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTIKSDDTIGRV
                     KAYEAIVKGENIAEPSIPESFKVLLKEMQSLALDVHVLSDEGREVEVREEDDELLRAA
                     EELGIDLSPATMRAAGREPTAEEVEGGAASADEAGD"
BASE COUNT          493 a          870 c          932 g          384 t
ORIGIN      
        1 tgaaccagcg actgggcgtg ggggtgcccg agagcacgcg ggttctcacc acggaggaca
       61 tcctggcgct cgttcgcaag cttgtcgagc tcccggacac gctcggcgtc cccgaggagt
      121 cgaaggacta cgcagccgac gctgccgggc ttccccggga tccgatccgc ggtgatctcg
      181 acgaatacga gcacttcggc aacaggcgcc tgcggacgac gggtgagctg atccaggagg
      241 cgttccgggt cggcctgtac cgcatggagc gcgtcgtgcg cgagcggatg acgaccgagg
      301 acgtcgacac gatcacaccg cagacgatca tcaacatccg gccggtcgta gcggccctga
      361 aggagttctt cggctcgtcg cagctgtcgc agttcatgga ccagacgaac tcgctggcag
      421 gcctcacgca tcgcaggcgg ctttcggcgc tcggggccgg cggcctgacg cgtgagcgcg
      481 cacccatcga ggtacgcgac gtgcacccga cgcattacgg gcgcatgtgc ccgatcgaga
      541 ccccggaggg ccccaacatc ggcctgatcg gctcgctcgc gtcgtacgcg accgtgtccg
      601 agttcggctt catccagacg ccgtacaggg tggtcaagaa cggcaaggtc ggcgacgaga
      661 tcgtctacct ggacgccgcc gaggaggcgc agttcaccat cgcgcaggcg tcggagccga
      721 tcgacccgaa gacgggcaag ttcctccacg accaggtgct ctgccgctct cgcgaaggcg
      781 aggccgtcgt ggccgacccg aagagcatcg agtacatgga cgtctcgccg gcgcagatcg
      841 tctccgtcgc aacggccctg atccccttcc tggagcacaa cgacgcgaac cgcgcgctga
      901 tgggcgcgaa catgcagcgg caggcggtgc cgctgatgat cccgcaggcg ccgctcgtcg
      961 gcaccgggct ggagtttcgg gccgccgtcg acaccggaga cgtcgtgctg gcagcggccg
     1021 atggctcggt catcgaggtc gacgccgagc acatcgttat cgaaggacgc ggcagccgca
     1081 acgagtatcc gctgacgaag ttcatgcgct cgaaccaggg aacgctcatc caccagaagc
     1141 ccgccgtgaa gctgggcgac cgtgtgaaga agggcagcgt cctcgccgac ggcagctcca
     1201 ccgacggtgg tgagctcgca ctcggggcga acctcctcgt ggcgttcatg ccttgggagg
     1261 gcttcaactt cgaggatgcg atcgtgatct ccgagcgtct cgttcgggac gacgtgctct
     1321 cgtcgatcca catccacgag tacgagatcg acgcccgctc gaccaagctg ggcgacgagg
     1381 agatcacgcg ggacatcccg aaccggtccg aggagtcgct cgcgaatctc gacgagcggg
     1441 gcgtcgtgcg catcggcgcc gaggtcacct cgggcgacct gctggtcggc aaggtgacgc
     1501 cgaagggcga gaccgagctg acggcggagg agaagctcat ccgcgcgatc ttcaaggaaa
     1561 aggcgcgcga ggtgcgtgac acgtcgctca aggttccgca cggcgagggc ggcaaggtga
     1621 tcggcgtcaa gacgttctcc cgtgaccttg gcgacgacct gccaccgggc gtcaacgagc
     1681 tcgtccgcgt ctacgtcgcg aagaagcgga agatcgcgga gggagacaag ctcgccggcc
     1741 gccacggcaa caagggcgtg atcgctcgaa tcgtcccgga agaggacatg ccgttcctcg
     1801 aggacggccg ctccgtcgac gtcgtgctca acccgctcgg tgtgccgagc cggatgaaca
     1861 tcggccagat cctggaaacg catctgggct gggccgccgc gcacggggtg ttcgcggacg
     1921 agaacggcgt ccgcgtgaac gatcgtgctc cgatcaacgc tcccgatccg cacccggtcg
     1981 cgaccgccgt cttcgacggc gcgactgaac aggacgtcga cgacgccctg gtggcgtggg
     2041 tgaagtcgaa ccccgacttc cccgtgaaga tgaacgtgga cgagaagcgc acagagggcc
     2101 ggaagtgctc gggcaaggtg cggctctacg acggcaagac cggccagcag ttcgagcaga
     2161 aggtcaccgt cggctacatg tacatcctca agctgctcca cctggtcgac gacaagatcc
     2221 acgcccgctc gaccgggccg tactcgctcg tcacccaaca gccgctgggc ggcaaggcgc
     2281 agttcggcgg gcagcgcttc ggggagatgg aggtctgggc gctcgaggcg tatggcgccg
     2341 cctacaccct gcaggagatg ctcacgatca agtccgacga cacgatcggc cgcgtgaagg
     2401 cctacgaggc gatcgtcaag ggcgagaaca tcgccgagcc gtcgatcccc gagtccttca
     2461 aggtgctgct caaggagatg cagtcgctcg cgctcgacgt ccacgtgctc tcggacgagg
     2521 gccgtgaggt ggaagtgcga gaggaggacg acgagctgct ccgcgccgcc gaggagctcg
     2581 ggatcgatct gtcgcccgcc acgatgcggg ctgcgggccg cgagccgacg gcagaggagg
     2641 tcgagggcgg tgcagcctcc gccgacgagg cgggcgacg
//
LOCUS       DBNGNK010000278         2591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_692104,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000278 DBNGNK010000000
VERSION     DBNGNK010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_692104"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="AC150V_08120"
     CDS             <1..271
                     /locus_tag="AC150V_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HIR3762925.1"
                     /translation="EIVWKYPQEEVRAFFSMSRGSNQRLPNGNTLICESDRGHVFEIT
                     PDGERVWEFWNPERGGRKNRRKAIYRFMRLSRDEVVRRMRGPASR"
     gene            423..1709
                     /locus_tag="AC150V_08125"
     CDS             423..1709
                     /locus_tag="AC150V_08125"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HIR3762926.1"
                     /translation="MRSRRRLQPVLGLAALALVAASCRAVPPGPYNLVLVTVDTLRPD
                     RLGFEGHARATSAAIDRLAAEGVSFERCYSQGGWTLPSIATILTGRYPSDHGAIDIDR
                     RMRGEIPTLAEILAARGYDTAAFVSHVFLGAEFGLGKGFDVYDASVLDVGHPHEVATA
                     EAITDRALEGLAALEPPFFLWVHYFDPHFKYLRHPEWAGFGRGRVDRYDGEIAHTDHH
                     IGRLLAALETEGVAERSVVVLTADHGEEFREHGGEYHYGLHEVDVRIPLVIKAPGLAP
                     RRDRAVAQQIDLMPTALDLLDVDSPSDLPGLSLVSGRRRDRPVFMERFRPVPFVQRAV
                     IHRDRKLYVVEVEGTRPEGLERVMAKTSVRPGISLFDLADDPAEANDLHATEPSSSEA
                     LLELLTSYVERSAPSGERHAVDAELTEKMRSLGYVQ"
     gene            1788..>2591
                     /locus_tag="AC150V_08130"
     CDS             1788..>2591
                     /locus_tag="AC150V_08130"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HIR3762927.1"
                     /translation="MGLLASALLIACGDGRKAPTPPSAIVLIVVDTLRADHLGAYDYE
                     RDTTPRIDDHAERGVVFERAWASSSWTLPTMASLLTGRLPTAHAAGRRSGTDPTRSSG
                     LDERTAMAAGVPVLAEVLRRDGFVTAAVASNPFLAERFGLQRGFVGYSNATGRRANEV
                     VDQALAWLGTHRDETFFLLVHVMDPHLPYDAPPPVRGRWAEGEDDLALRYVKAIRRQA
                     KDLGPERRAGLVDRYDEEIAFVDGEIGRLLDAIRALDRWTETLVVLTADH"
BASE COUNT          366 a          959 c          878 g          388 t
ORIGIN      
        1 cgagatcgtc tggaagtatc cgcaggagga ggtccgcgcc ttcttcagca tgtcgcgcgg
       61 ctcgaaccag cggctgccca acggcaacac gctgatctgc gagtccgacc gcgggcatgt
      121 cttcgaaatc accccggacg gagagcgcgt ctgggagttc tggaacccgg agcgcggcgg
      181 gcgcaagaat cgtcgcaagg cgatctaccg cttcatgcgg ctgtcgcggg acgaggtcgt
      241 ccggcgcatg cggggcccgg cctcgcgcta gttggtcgcc gcacgcatcg cacgagccgt
      301 ccgtcttgac cgtgagccgt ctcgagcaag cgtcggggac tccccaatcg gacggctacg
      361 ccgcttgggc cgtaccgtcg cacggtcgcc tcgcggcgag cgggacgggc taggatgagc
      421 gcatgaggtc gcgacgtcgc ctgcagccgg tgctgggtct cgcggccctc gcgctcgtcg
      481 ccgcttcctg ccgtgcggtt ccgccagggc cctacaacct cgttctcgtc acggtcgata
      541 ccctgcgtcc ggaccgcctc ggcttcgaag ggcacgcgcg cgccaccagc gccgccatcg
      601 accggttggc ggccgagggc gtgagcttcg agcgctgcta cagccagggg ggctggacgc
      661 tgccctcgat cgcgacgatc ttgaccgggc gctaccccag cgaccacggc gcgatcgaca
      721 tcgaccgcag gatgcggggg gagatcccga ccctggcgga gatcctcgcg gcacgaggct
      781 acgacaccgc ggcgttcgtg agccacgtct tcctcggcgc cgagttcggt ctgggcaagg
      841 gcttcgacgt ctacgacgcc tcggtcctgg acgtgggaca tccgcacgag gtggcgaccg
      901 ccgaggccat caccgatcga gcgctggagg gtctggccgc cctcgagccg cccttcttcc
      961 tctgggtcca ctacttcgat cctcacttca agtacctgcg ccacccggag tgggccgggt
     1021 tcggccgcgg gcgcgtcgat cgctacgacg gcgagatcgc ccacaccgac caccacatcg
     1081 gccggctgct cgccgcgctc gagaccgagg gtgtcgccga gcggagcgtc gtcgtgctga
     1141 ccgcggatca cggagaggag ttccgggagc acggcggcga gtatcactac gggctgcacg
     1201 aggtcgacgt gcgcatcccc ctggtgatca aggcgcccgg cctggcgccg cgccgcgacc
     1261 gggccgtggc ccagcagatc gacctcatgc ccaccgctct cgacctgctc gacgtcgact
     1321 cgccctccga cctgccgggg ctgtcgctcg tctccgggcg ccgccgggac cggccggtgt
     1381 tcatggagcg gttccgtccc gtccccttcg tccagagagc cgtcatccac cgcgaccgga
     1441 agctctacgt ggtcgaggtc gaggggacgc gtcccgaggg gctcgagcgc gtcatggcca
     1501 agaccagcgt ccgcccgggc atcagcctct tcgacctcgc cgacgatccc gccgaggcga
     1561 acgacctcca cgccaccgag ccatcctcgt ccgaggcgct cctcgagctg ctgacgagct
     1621 acgtcgagag gtccgcgccg tccggcgagc gccacgcggt cgacgccgag ctgacggaga
     1681 agatgcgcag cctcggctac gtgcagtagc tcaccaccgg catcatgcgc tccccatccg
     1741 acccaaacca ctacgcttcg accggcactg cgcctctcat gctgctaatg ggcctgctgg
     1801 cctcggcgct gctgatcgcc tgcggggacg gccggaaggc gccgacgccg ccctcggcca
     1861 tcgtcctgat cgtcgtcgac acgcttcgag ccgatcacct cggcgcctac gactacgagc
     1921 gcgacaccac gccccgcatc gacgaccacg ccgagcgcgg ggtggtcttc gagcgagcct
     1981 gggcgagctc ttcgtggacg ctgccgacca tggcctccct gctgaccggc cgcctgccga
     2041 ccgctcacgc cgccggccgg cgttcgggca ccgacccgac gcgcagctcc ggcctcgacg
     2101 agcggacggc gatggcggcg ggcgtgccgg tgctcgccga ggtcctgcgg cgcgacggct
     2161 tcgtcaccgc cgccgtcgcc agcaacccgt tcctcgccga gcgcttcggt ctgcagcgcg
     2221 gctttgtcgg ctacagcaac gcgaccggcc ggcgggcgaa cgaggtggtc gatcaggcgc
     2281 tcgcctggct cggcacgcac cgcgacgaga ccttctttct cctcgtgcac gtgatggatc
     2341 cccatctgcc ctacgacgcg ccaccgccgg tccgcggccg ctgggccgag ggggaggacg
     2401 acctcgcgct gcgctacgtc aaggcgatcc gccggcaggc gaaggatctc ggtccggagc
     2461 gtcgcgccgg tctggtcgac cgctacgacg aggagatcgc cttcgtcgac ggcgagatcg
     2521 gccggctgct cgacgcgatc cgggctctgg atcgctggac cgagacgctg gtcgtcctga
     2581 ccgcggatca c
//
LOCUS       DBNGNK010000279         5586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1480555,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000279 DBNGNK010000000
VERSION     DBNGNK010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1480555"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..656
                     /locus_tag="AC150V_08135"
     CDS             159..656
                     /locus_tag="AC150V_08135"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR3762928.1"
                     /translation="MAAAAWFFATIAGVTPLTYDVPGAAAGAMVFLTLNLYFLITAIG
                     RIRSARYAADRRASVRLTVSLHGRIDGAPCEIVDVSLTGAAVHVDRPWKSRTGTVRPD
                     IDFAEECVSLEARIRHREQDACGTELGLEFLPGRRHAIFRVALALLSSEAAPRSERTS
                     IVRAA"
     gene            751..1245
                     /locus_tag="AC150V_08140"
     CDS             751..1245
                     /locus_tag="AC150V_08140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3762929.1"
                     /translation="MSEEAGDKRTLLNLTTATKDNALTEKERNAFLERKLVARLGTNR
                     RDGWWHVTPIWYIWDEGTFFLSLGNSRRHLRNMREDEHVTLCIDVDKRPEGVMEGPVS
                     VVCFGTAELVEEPTRVREITDRLELRYLGSIPPEFEEAMWFEGRTAVVITPVRWLAWD
                     QGKS"
     gene            1358..1654
                     /locus_tag="AC150V_08145"
     CDS             1358..1654
                     /locus_tag="AC150V_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HIR3762930.1"
                     /translation="MDTYAIVRRRGWRTPDELHEAAERSRAVGNEEMPDKVRWIRTYT
                     LEEDDGTLGAVCIYQATDLDAIREHSTRAQLPDPEIVPIDDTIVVRPDPEGVKV"
     gene            complement(1676..2593)
                     /locus_tag="AC150V_08150"
     CDS             complement(1676..2593)
                     /locus_tag="AC150V_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3762931.1"
                     /translation="MKLGFLTVPFGDMSLEEVAAWAEGRGFTALEIACWPPASGDTRR
                     YAGVSHIDVGSLDDAEANQVLAMLDGHGIEISALGYYPNNLHPDPSERQAANDHLRKV
                     IEAAPKLGVDVVTTFIGNEKDRSLPENLETFKEVWPLLVEFAEQHGVKIAIENCPMIF
                     SYDEWPGGNNLGYSPAIWRQMFETIPSASFGLNLDPSHLVWQMIDYERCVYDFADRIF
                     HVHAKDLEIRRDGLYEHGVMSLGIGWQVPRIPGLGEVRWDRFIAALYAVGYDGPVIIE
                     HEDRTFEGTRELVERGFDVARDVLSPYIV"
     gene            complement(2714..2986)
                     /locus_tag="AC150V_08155"
     CDS             complement(2714..2986)
                     /locus_tag="AC150V_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762932.1"
                     /translation="MTRTIITKFAVLTSLALIALIAVQGAAAKTLPKTTVVVSAALAK
                     IQHPGTVSGIAGYESYRGGPTGVYEGQTIEIPADLSYIQHPGTVTG"
     gene            3091..4653
                     /locus_tag="AC150V_08160"
     CDS             3091..4653
                     /locus_tag="AC150V_08160"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3762933.1"
                     /translation="MEFRILGPLEVVEDGESLALGGERQRAVLALLLTRPNEVVSTDK
                     LIDDLWGSEPPKTAANTVQYYVSQLRKLLGAERIETRTPGYAIRVETGELDLQRFERL
                     LSQGDGDALREALTLWRGPALADLRYESFAQAEITRLDEMRLVALEKRIDADLAAGKD
                     AELVGELEAVVEEHPLRERPRGQLMLALYRAGRQADALAAYSAARKTLVDELGIEPSS
                     ALQELERAILRQDPSLLVETAEAPAAPQRSLLVLPANLELVDALLSIAVPLAARPHRE
                     LIVARLVSRSEDLATTTAALAERREALATEGVTARIVSYTSTSPGEEAVTLAAEQDVD
                     LVLVDAPVELLESGELDPDLLAVLQSAPCDVGVLAKAGELTAKRSGPVVVPFGGVEHE
                     WSAIEIAAWIARALGVPLRLAGTSDVGGRRDASKLLGRASLVVQAALGIVAEPALVPA
                     GAKGMLEAAEDASLLVVGLSERWQKEGLGEARLALAREASVPTLLVRHGLRPGGLAPP
                     ETMTRFTWTLAR"
     gene            complement(4666..>5586)
                     /locus_tag="AC150V_08165"
     CDS             complement(4666..>5586)
                     /locus_tag="AC150V_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR3762934.1"
                     /translation="GSTPTGIAVAGDTVWVVNFSDRTVSAIDASSNTVVKTPGLDGGL
                     PSRSAGLFAVDADEEGAWVITQTGLARGSLWRLRLDSNAQKFPIEYEPNSVAVSENWV
                     WLTTRTGELLRVDPGTGTVDKVIEEEQPVQQALVGDEGALWTVSGSGLVSEGTDVTVT
                     IARRDPDTGEITVQESVRRGNEQFDAFFDVCAGAVWVHQPQQERILVLDAENLRRIGE
                     IGGLQPVLQNLGQIDCSDSAVWRLDSANENLELVEPRIQRLDPESREVEAEIAIPKIA
                     APEFGLQPLPTRLAIGADGVWITIGPPPLG"
BASE COUNT          854 a         1882 c         1951 g          899 t
ORIGIN      
        1 gcacgacgcc gcggcccgcg aacgctctgg aaggctcgaa ttcgtgccga tcagtgtggg
       61 gtggtggcta cccgcacaaa cagcggagcg gcggttcacg gcatcgcagc gatgcttccc
      121 ggctttccgg gggccgccag gccccctgcc gagcctgcat ggccgcggcg gcctggttct
      181 tcgcgacgat cgccggcgtg acgccgctca cctacgacgt gcccggcgca gctgccggag
      241 ccatggtgtt cctcaccctc aacctctact tcctgatcac ggcgatcggc cgcatccgct
      301 ccgcccgcta cgcggccgac cggcgggcca gcgtgcgcct gacggtgtct ctccacggcc
      361 ggatcgacgg agcgccgtgt gagatcgtgg acgtctcgct caccggcgcc gccgtacacg
      421 tcgatcggcc ttggaagtcc cgtacgggca cggtccgccc ggacatcgac ttcgcggagg
      481 agtgcgtctc cctcgaggcc aggatccgcc accgcgagca ggacgcctgc ggaacggagc
      541 tcggcctgga gttcctcccc ggccggcgac acgccatttt ccgggtcgcc ctggcgctcc
      601 tgagctcgga agcggcgccc cggtcggagc ggacgtcgat cgtccgcgcg gcctgagctc
      661 gaccattcct cgtttgcact ccggcctgcg gttgccgagg tcgtcacatc gccgtaggct
      721 cgccgacgta agcgtcctga aggagacgac atgagcgagg aagccggaga caagcgcacg
      781 ctgctgaacc tgaccacggc gacgaaggac aacgcgctca ccgagaagga gcggaacgcc
      841 ttcctcgagc gcaagctcgt cgcgcgtctc ggcacgaacc ggcgcgacgg ctggtggcac
      901 gtgacgccca tctggtacat ctgggacgag ggaacgttct tcctctcgct cggaaacagc
      961 aggcgccacc ttcggaacat gcgcgaggac gagcacgtca ccctctgcat cgacgtcgac
     1021 aagaggcccg agggcgtgat ggagggcccc gtctcggtcg tctgcttcgg aacagcggag
     1081 ctggtcgagg agccgacccg agtacgcgag atcacggatc ggctcgagct gcgctacctc
     1141 ggatcgatcc cgcccgagtt cgaggaggca atgtggttcg agggccggac ggcggtggtc
     1201 atcaccccgg tccgctggct ggcctgggat cagggcaaga gctgacccgt ggccgccggg
     1261 gggtggaccg gcggctgagg ggtacatggc gccttgaccg gccggggtcg gcgcgcgacg
     1321 atgcatgccc gaccgcccgc actcgaggag gacagcgatg gacacgtacg cgatcgttcg
     1381 ccgcagaggc tggcgcaccc cggacgagct ccatgaggcg gccgagcgct cgcgcgcggt
     1441 cggcaacgag gagatgcccg acaaggttcg ctggatcagg acgtacacgc tggaggagga
     1501 cgacggcacc ctgggcgccg tctgtatcta ccaggcgacc gacctggacg ccatccgcga
     1561 gcattccacc cgcgcccagc tccccgatcc cgagatcgtc cccatcgacg acacgatcgt
     1621 cgtccggccc gacccagagg gggtcaaggt ctagacgcgc cccggcgtcg ggaagtcaga
     1681 cgatgtacgg gctgagcacg tcgcgggcga cgtcgaagcc ccgctcgacg agctcgcgcg
     1741 tgccttcgaa cgtgcggtcc tcgtgctcga tgatgacagg gccgtcgtag ccgaccgcgt
     1801 acagcgccgc gatgaagcgg tcccagcgca cttcgcccaa gcccgggatc cgcggcacct
     1861 gccagccgat gccgagcgac atgacgccgt gctcgtagag gccgtcgcgt cggatctcca
     1921 gatccttcgc gtggacgtgg aagatccggt cggcgaagtc gtagacgcag cgctcgtagt
     1981 cgatcatctg ccagacgaga tgggacgggt cgaggttcag gccgaagctc gcggacggga
     2041 tcgtctcgaa catctgccgc cagatcgcgg gcgagtagcc caggttgttg ccgcccggcc
     2101 actcgtcgta gctgaagatc atcgggcagt tctcgatcgc gatcttgacg ccgtgctgct
     2161 cggcgaactc gacgagcagc ggccagacct ctttgaaggt ctccaggttc tcgggcagcg
     2221 agcggtcctt ctcgttgccg atgaaggtcg tgacgacgtc gacgccgagc ttcggcgcag
     2281 cctcgatcac cttgcgcaga tggtcgttgg cagcctgccg ctccgacggg tcggggtgga
     2341 ggttgttcgg gtagtagccg agcgcggaga tctcgatccc gtgcccgtcg agcatcgcga
     2401 ggacctggtt cgcctcggcg tcgtcgaggg agccgacgtc gatgtgcgag acgccggcgt
     2461 agcgccgcgt gtctcctgac gcgggcggcc agcaggcgat ctcgagcgcg gtgaagccgc
     2521 ggccctcggc ccaggccgcg acctcctcga ggctcatgtc cccgaaggga acggtcagga
     2581 agccgagctt cacgacgacg aatctacagc agtcgcttcc gcgatgtcct ggatgcgggc
     2641 gcgcaggaaa cgggccgccc gaccgcccgt tcggcggccg gcgcggcccg tccgcgccgg
     2701 atctgcgcgt gtctcacccg gtgaccgtgc ccggatgctg gatgtacgag aggtcggcgg
     2761 ggatctcgat cgtctggcct tcgtagacgc cggtcgggcc gccgcggtag ctctcgtagc
     2821 cggcgatgcc ggagacggtg ccggggtgct ggatcttggc gagggccgct gagacgacga
     2881 cggtcgtctt cggcagcgtc ttcgccgccg cgccctgcac cgcgatcagc gctatcagcg
     2941 cgagagaggt gaggactgcg aacttcgtga tgatcgtgcg ggtcatgatt ccctcctgct
     3001 cggtgcgacc actccgtgtg accgcctgtg ctgcaaagat gcgcccgagc gttgagtgct
     3061 cactgagagg tcgctgagag gagcgctgag gtggagttcc gcatcctcgg tccgctcgag
     3121 gtcgttgagg acggtgagtc ccttgccctg ggcggtgagc ggcagcgtgc cgtcctggcc
     3181 ctgctgctga cgagaccgaa cgaggtggtc tcgacggaca agctgatcga cgacctgtgg
     3241 ggctccgagc cgccgaagac cgcggccaac accgtgcagt actacgtctc ccagctgcgc
     3301 aagctgctgg gagcggagcg gatcgagaca cggacacccg gctacgcgat ccgcgtggag
     3361 accggggagc tcgacctgca gcgcttcgag cggctgctct cccaggggga cggcgacgcc
     3421 cttcgcgagg cgctcacgct ctggcgcggg ccggctctcg ccgacctcag gtacgagtcg
     3481 ttcgcgcagg ccgagatcac ccgcctcgac gaaatgcggc tcgtcgcact ggagaagcgg
     3541 atcgacgcgg atctcgcggc ggggaaggac gccgagctcg tgggcgagct cgaagcagtc
     3601 gtcgaggaac acccgctgcg cgagcgtccg cgaggtcagc tgatgctcgc gctgtaccgc
     3661 gcgggacgcc aggcggatgc gctggcggcc tactcggcgg cacggaagac gctggtcgac
     3721 gagctgggca tcgagccgag ctccgcgctg caggagctgg agcgcgcgat cctgcggcag
     3781 gacccctcgc tgctcgtcga gacggccgag gcgcctgccg cgccacagcg atcgctgctc
     3841 gtgctgccgg cgaacctgga gctcgtggac gcgctgctgt cgattgccgt tcccttggcc
     3901 gcgaggcccc accgagagct gatcgtcgcg cgcctggtct cccgtagtga ggatttggcg
     3961 acgacgaccg cggcgctggc ggagcgtcgg gaggcgcttg cgaccgaggg cgtgacagcc
     4021 cggatcgtct cgtacacgtc gaccagcccg ggcgaggagg ctgtcacgct cgccgcggag
     4081 caggacgtcg atctcgtcct cgttgacgcg ccggtggagc tgctcgagag cggtgagctc
     4141 gatcccgacc tccttgcagt gctgcagtcg gctccctgtg acgtcggcgt gctcgcaaag
     4201 gccggggagc tcacggcgaa acgctcaggg ccggtcgtgg tgccgttcgg cggcgtcgag
     4261 cacgagtggt cggcgatcga gatcgccgcc tggatcgcgc gggcactcgg tgtgccgctc
     4321 cgcctggcag ggacgtcaga cgtgggtggc cgccgcgacg ccagcaagct gctcggccgg
     4381 gcgtcgctcg tcgttcaggc cgcgctcggc atcgtcgccg agccggcgct ggtgccggcc
     4441 ggggccaagg gaatgctcga ggccgccgag gacgcgtcgc tgctcgtcgt cggcctctcc
     4501 gagcgctggc agaaggaggg cctgggcgag gcacggctgg cgctcgcccg cgaggcgtcc
     4561 gtccccacgc tgctcgtccg ccacggtctc cgtcccggcg gtctcgcgcc acccgagacg
     4621 atgactcgct tcacctggac gctcgcgcgc tgaggctcgc ccccctcagc ccagaggtgg
     4681 cgggccgatg gtgatccaga cgccgtctgc tcctatcgcc agtcgtgtcg ggagcggctg
     4741 tagtccgaac tcaggcgcag cgatcttggg gatcgcgatc tctgcttcga cctcgcgaga
     4801 ttctggatcg agtcgttgga tacgcggttc gactaactcg aggttttcgt ttgcgctgtc
     4861 tagcctccaa acagcactat cgctgcagtc gatctggccg aggttctgta agacgggttg
     4921 caacccgccg atctcgccga tccggcgcag gttctcggca tcgagcacca ggatccgctc
     4981 ctgctgaggc tggtgcaccc acacggcacc ggcgcagaca tcgaagaatg cgtcgaactg
     5041 ctcgttgccg cgccgaacgc tctcttgaac cgttatctcg ccggtgtcgg gatccctgcg
     5101 cgcaatcgtc accgttacgt cggtgccctc tgacacgaga cccgaccctg aaacggtcca
     5161 aagcgcaccc tcgtcaccca ccaacgcctg ttgaaccggc tgttcttcct cgatgacctt
     5221 gtcgacggtt ccggttcccg ggtcgacacg gagcagctcg ccggttcgtg tcgtcagcca
     5281 gacccagttc tcgctaacgg caaccgagtt cggctcgtac tcgatcggga acttctgcgc
     5341 gttcgagtcg agacggagac gccagaggct cccccgggca aggccagtct gggtgatgac
     5401 ccaggcgccc tcttcatccg cgtcaacggc gaacagtccc gcgcttcgtg atggcagtcc
     5461 gccgtcgagc ccaggggtct tgacgacggt gttcgagctc gcgtcgatcg cggagaccgt
     5521 ccggtcgctg aagttgacga cccagacggt gtcgccggct acggcgatgc cggtaggagt
     5581 gcttcc
//
LOCUS       DBNGNK010000280         3378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_979157,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000280 DBNGNK010000000
VERSION     DBNGNK010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_979157"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="AC150V_08170"
     CDS             <1..1183
                     /locus_tag="AC150V_08170"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR3762935.1"
                     /translation="LTDQLVVVTSPDAAELYEGVRVAVQERPLGTGDAVRSARVAAGD
                     ASHVLVLSGDTPLLTDQLLSELLATHRDTGAAATVLTFRPDDMRSYGRVVRNPDGGVA
                     AIVEAADATPEQLAVDEANSSIYVFEAGLLWPALDRLEAHNAQGELYLTDAVRDLASS
                     GHRVVAHVAPEAAETEGVNTRVELAAAAAALRDRLNEQHMLAGVTVVDPATAWIEPTV
                     TLEPDAVIHPFTSLRGTTHVSAGAEIGPHVVAVDARIGPAALVGPFCYLRPGTVLEAA
                     AKAGTFVEIKNSWVGEGAKVPHLAYMGDADVGEGTNVGAGSITANLSHHPERGKTRTK
                     LGRNVKVGVDTMFVAPVTLGDEAWTAPGTVVTDDVPDNALVGFPPRQTIKEGRGGKRD
                     D"
     gene            1206..2216
                     /locus_tag="AC150V_08175"
     CDS             1206..2216
                     /locus_tag="AC150V_08175"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR3762936.1"
                     /translation="MSSPGADSQAGRAIGLAPKKRLTIVSGRSNPELAQRIAEQLGCE
                     LGEVTLKTFANGETYCRYDESIRGADMFLVQTASRPVDQHLIELLIMINAAKLASAKR
                     ITAVVPWYFYGRQDKKSRPREPITARLVADMLQTAGADRVLTMDLHAGQVQGFFHIPV
                     DHMTAVPMFAQYFRDLGGDSELAAVSPDTGRTKLAGKFAEMIGGDLVVLNKERPEHNV
                     ARVSTVIGDVEGKVAVMTDDIVDTAGTIVQGAIALKEAGATKVYACATHGLFNGPAIE
                     RIAESEIDKLVVTDTVPVDPLAKPDNVEVISVAGILAETIRNVFSDDSVSAIFAGENQ
                     LF"
     gene            2417..2866
                     /locus_tag="AC150V_08180"
     CDS             2417..2866
                     /locus_tag="AC150V_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR3762937.1"
                     /translation="MARFAPRLAPPRRVGNESGLTLVEMLIVLGIIGVLLAIAVPHYI
                     SFADHANDAVAKTNLKTLALVAEIYYTDNDGTPSDADGDSTTAGYEGMTMAILKGYNA
                     ALPASLSFNGTPTIASYCARTLHGGTWWSARGPGIGADSFKKNDNCA"
     gene            2959..>3378
                     /locus_tag="AC150V_08185"
     CDS             2959..>3378
                     /locus_tag="AC150V_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_391899485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3762938.1"
                     /translation="MPLEVDIDVGLLKSEIRKTYAAVSTEPERDFIFPTGREWAADLG
                     YPADLLERVPGGAVESYAGVANPFALGPLQPGERVADLGCGAGTDSLVAAQMVGADGH
                     VTGVDMTREMIEKARAAAAEMGVANVEFTQGEVESLPF"
BASE COUNT          577 a         1178 c         1118 g          505 t
ORIGIN      
        1 cctcaccgac cagctcgtcg tcgtgacctc tccagacgcc gcggagctct acgagggagt
       61 cagggtcgcc gtacaggagc ggccgctcgg taccggtgac gcggtccgca gcgcgcgcgt
      121 ggcggcgggc gacgcgagcc acgttctcgt gctctcaggc gacacgccgc tcctcaccga
      181 ccagctcttg tcggagctcc ttgcgaccca ccgagatact ggcgccgcgg ccacggtgct
      241 gacgttccgg ccggacgaca tgaggagcta cggcagggtc gtccgcaacc ccgacggcgg
      301 cgtcgccgcg atcgtcgagg ccgcggacgc gacgcccgag cagctcgcgg tcgacgaggc
      361 gaactcttcg atctacgtct tcgaggccgg gcttctgtgg ccggcgctgg accgcctcga
      421 ggctcacaac gcgcagggtg agctctacct caccgacgcc gtgcgggacc tcgcgtcctc
      481 cggccatcgc gtcgtggccc acgtcgcgcc ggaagcggcg gaaaccgagg gagtcaacac
      541 gcgagtcgag ctggctgcag ccgcggcggc gctgcgcgac cgcctgaacg agcagcacat
      601 gctcgccggc gtgaccgtcg tcgaccccgc gacggcgtgg atcgagccca ccgtcacgct
      661 cgagcccgac gcggtcatcc atccgttcac gtcgctccgt ggcacgacac acgtctccgc
      721 cggcgcagag atcggcccac acgtcgtcgc cgtcgacgcc cggatcggac ccgctgcgct
      781 cgtcggcccc ttctgttacc ttcgccccgg gacggtgctc gaggcggctg cgaaggcagg
      841 cacgttcgtt gagatcaaga actcatgggt cggagaaggg gccaaggttc cccacctcgc
      901 ctacatgggc gacgccgatg tcggcgaggg gacgaacgtg ggcgccggct ccatcacggc
      961 gaacctctct catcatcccg aacggggcaa gacgcgtacg aaactcggtc gtaacgtcaa
     1021 ggtgggagta gacactatgt tcgttgctcc ggtcactctg ggagatgagg cgtggacggc
     1081 gccgggaacc gtcgtcaccg acgacgtccc cgacaacgcg ctcgtcggct tcccgccgcg
     1141 acaaacgatc aaggaaggta ggggtggaaa gcgggacgat tgagcagacg ctcccgggtc
     1201 tcgcgatgag cagccctggg gctgactcgc aggccgggcg ggccatcggc ctcgcgccga
     1261 agaagaggct cacgatcgtc tccggccgct cgaaccctga gctcgcgcaa cgcatagccg
     1321 agcagctggg ctgcgagttg ggcgaggtga cgctcaagac gttcgcgaac ggcgagacat
     1381 actgccgcta cgacgagtcc atccgcggcg ccgacatgtt cctcgtccag acggcctctc
     1441 ggccggttga ccagcacctg atcgagctgc tgatcatgat caatgccgcg aagctcgcct
     1501 ccgcgaagcg catcaccgcc gtcgtcccct ggtacttcta cggccggcag gacaagaagt
     1561 ctcggccgcg cgagccgatc accgcgcgtc tcgtagcgga catgctccag accgccggcg
     1621 ccgaccgcgt gctcacgatg gacctccacg ccggccaggt gcaggggttc ttccacatcc
     1681 ctgtcgatca catgaccgcg gtgccgatgt tcgcccagta cttccgcgac ctgggcggtg
     1741 actcggaact cgccgcggtc tcccccgaca cgggtcgcac caagctcgcc ggcaagttcg
     1801 ccgagatgat cggaggcgac ctcgtcgtcc tcaacaagga gcgtccggag cacaacgtcg
     1861 cgcgcgtctc cacggtgatc ggcgacgtcg aaggcaaggt cgcggtcatg acggacgaca
     1921 tcgtcgacac cgccgggacc atcgttcagg gcgcgatcgc gctgaaggag gcaggcgcta
     1981 cgaaggtgta cgcctgcgcc acgcacggac tcttcaacgg cccggcgatc gagcgcatcg
     2041 cggagagcga gatcgacaag ctcgtcgtga ccgacacggt gcccgtcgac ccgctggcga
     2101 agcccgacaa cgtcgaggtc atctccgtcg cgggcatcct cgcggagacc atccggaacg
     2161 tcttctccga cgactcggtg tccgcgatct tcgcaggcga gaaccagctc ttctagcctg
     2221 acgccattgg ccggcggggg caccgtccgg cacagccggg gtcgccactc ctgacgccgc
     2281 gcgctggacg actctgatcg aatcgggcgc tcgtttcggc tcgtccgggc agtagaacaa
     2341 gccgggcagt agaacaagaa cgacacgtac ctcttccggg ggaactgcga tcccccgttc
     2401 ggggcgatca gggggtatgg cccgatttgc gccgcgtctc gcgccgcccc gtcgcgtcgg
     2461 aaacgagtca gggctcaccc tggtcgagat gctgatcgtc ctcggcatca tcggagtgct
     2521 gctcgcgatc gccgtccccc attacatctc ctttgcggat cacgccaacg acgccgttgc
     2581 gaagacgaac ctcaagacgc tcgccctggt ggcggagatc tactacaccg acaacgacgg
     2641 cacccccagc gacgccgacg gcgacagcac gacggccggc tacgagggca tgaccatggc
     2701 gatcctgaag ggctacaacg ctgcgctccc cgcctccctg agcttcaacg gcacgccgac
     2761 gatcgccagc tactgcgcgc ggacgttgca cggcggcacc tggtggagcg ctcggggccc
     2821 tgggatcggt gccgactcgt tcaagaagaa cgacaactgc gcgtagttgg gccgcgttcg
     2881 cgcgtctggg aaaccggcga ccggcgggct atgatcgcga tcgcagtctg ggccagccgc
     2941 gcgacacgga ggtgacgaat gccgctcgaa gtcgacatcg acgtcgggct cctgaagagc
     3001 gagatccgta agacctacgc tgccgtctcg accgagccgg agcgcgattt catcttcccg
     3061 accggccgcg agtgggcggc cgatctgggt taccccgccg atctcctgga gcgtgttccc
     3121 ggcggagcgg tcgagtccta cgccggagtc gccaatccat tcgcgctcgg cccgcttcag
     3181 cccggggagc gggtcgccga cctcggctgc ggcgccggca ccgattcgct cgtcgcagcg
     3241 cagatggtcg gagccgacgg ccacgtcacg ggcgtcgaca tgacccgcga gatgatcgag
     3301 aaggcacgcg ccgcggccgc agagatgggc gtcgcgaacg tcgagttcac gcagggagag
     3361 gtcgaatcgc ttccgttc
//
LOCUS       DBNGNK010000281         6945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_979294,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000281 DBNGNK010000000
VERSION     DBNGNK010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6945
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_979294"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC150V_08190"
     CDS             complement(<1..274)
                     /locus_tag="AC150V_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762939.1"
                     /translation="MADSGNRIVAGALSALAPGAGQAYAGAIRRGVVLLVLAVVPLAA
                     LAAMVAAERDALLDAAFTRNALAAVLVADLALLALRWFAVVDAWRLG"
     gene            373..768
                     /locus_tag="AC150V_08195"
     CDS             373..768
                     /locus_tag="AC150V_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3762940.1"
                     /translation="MSGYTIKPLSEVPDVLGDYPGEMQMITKEIGAEQSALTYRRMPP
                     KTGGKGSYGHRHKTQEELYFVISGALQFKLGDDVVDVPAHTAVRVAPQVWRSVWNDGP
                     GDAELFIVSPRVDDPRADTESIEDFWPED"
     gene            complement(784..5484)
                     /locus_tag="AC150V_08200"
     CDS             complement(784..5484)
                     /locus_tag="AC150V_08200"
                     /inference="COORDINATES: protein motif:HMM:NF053729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP-binding domain-containing protein,
                     MshE-related"
                     /protein_id="HIR3762941.1"
                     /translation="MSSRLIGAIFVEKGLITEEQLSEALEQQRASGERVGEILVSKFG
                     VSRLALASALAEQWAEYERPRTGGSAPEIDKSDLAALSAEWPGTTPAVPEEMIARQPI
                     GEIFRERGLLTEEQLEEALAAQAKSGQRLGEILVAKGVVSRLELASALADQWSTLQKL
                     RPPASEREGQGEPGPAQDASGTSEPSPAASEAGTGEALAPLRADIDAIAARLERDDRA
                     DIDLAQIEERLAALERSHMDSGVLDELRGELVELRERIATLGPPPDGAAADFDLEPLR
                     ERLVALEQKTGETDDFERLRARIDGLAARIAAVPQFDDAELVTRLDAVEGRLQIAAAS
                     ASALDDRVSSALAGATDGLHVEVDQLRSRMASIESSSGSIEELRTELSSLEQRIGEST
                     QLRAELAALAERVDSIPTSFEARIEEIVARAAETTAARADLDSVTQQIEALVEAQSSA
                     PTAASADLEEIRSAIAGLDERVAAAARVHAEVAGLADQLEALPGSLESRLEDFSERIA
                     QTDTLPAKLGELQTQLEALATRTPESSVPGASDLEALRTSISLLEARLGDADAIGAEM
                     RALSERVDSIPSTFESQVEALTARVEEVALGGNALARLEERVDELASELAGASQLRVG
                     DVEQLRTTLEEVASGRREVEESLQRRIGELERKLEVSNRAEIDVHAELRPVVRDATEP
                     VHAELRELRTRLDESSTLRGALDAMGERLQKLEQDGGAAEFEQRARGLEATIDAAAAA
                     LREEQEELRARIENGDRERGRIDEVERRLAEMDDRLAAAAELEQQLTGSLEALGEEAT
                     TRARQGDDELRSSLTGLIQESAEAAHAARIALTNRVDELAAEIAAAAELTGRVDALQG
                     RLDAGEMRDPRVEELERRLEEGAEVEGRLRGMVAPLEEAADAIQADQAALRQALGQAL
                     NQLSSVDSVAGRLADLERRLAEGEELESRLRESLSGLVVESASSVQEEQATLRAEQTE
                     LRERMENLAAAAESASNDLSERVAQAERRLAESEGIEPRLQASLSGRVIQSVNELQGG
                     QTELRDQQAELQARQIELSEQIDMLGEETRALRALSDRVAAAELRLQEGGEAEKRLGD
                     SLSRLVRETRDELLAEYAALRAGQKETAARLDEIEVLRERADDADERLGGVEPLRARL
                     AAVDERLAEAEKGLVGSDRLAELEDRIARLATSSSRDTDELRNRLEELRDSVGAELAS
                     AAARAVDETARQAAAALERHTAHHETLEAQIAEADVRDEFASFVQDTERRLDELSERI
                     TSGATEALQASRRVDEVAHRAGEAVEALKSELAATKDELASGVAETHETLSTTVGEIE
                     SRIDDGARRSAERSAVVEQALRRELAAQRQALEAQADDARAQAESIDALASAAEERDA
                     ASVDAREELQEELERLSSSFGWRIERVEEVLARNETDELRHVVEELAERLDAQSAVSQ
                     EQVRVTEKALRKGLASLGARLAESEEAYVDAGNALRRSIERLGLAIEQADDRIEQRES
                     PPANSRYVEGFVAFAPTGDGYRLLEVAGPLPDVGGELTIPEADGLLRVTRIGESPLPL
                     DVRPCAFLERNPAE"
     gene            5684..5950
                     /locus_tag="AC150V_08205"
     CDS             5684..5950
                     /locus_tag="AC150V_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762942.1"
                     /translation="MTHTIQIECAQAVDGDELLAAFAAQGLPGEIVGREPRLVFRLGD
                     AGRDARRFAFDVAHALDGWVAERHVALVPMSIGDDAFVLRPPAA"
     gene            5972..6577
                     /locus_tag="AC150V_08210"
     CDS             5972..6577
                     /locus_tag="AC150V_08210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3762943.1"
                     /translation="MQVARIAPGLWRWTARHPSWTPADGGPDGWEAEVACVYWEAPDA
                     VVLVDPLVPAEPPDRELFWQALDRDVDRLGLPVVIVLTCAWHARSTEEILSRYEARLR
                     EGSEPLPGTAIGFPAGEETILWVPDAGALVIGDVLVGNRSGGVRLCPASWLHGGTPTA
                     DVAASLRPLLDLPVEHILVSHGDPVLGGGATALRRALSDAF"
     gene            complement(6581..6865)
                     /locus_tag="AC150V_08215"
     CDS             complement(6581..6865)
                     /locus_tag="AC150V_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR3762944.1"
                     /translation="MVRRAAGLLERRWTVSILYASYEGASRFNEFRQAVGRIPPRTLA
                     QRLVELEAAGVLEREVIDARPPRVEYRLTPEGRRLRRVVDALAQWAGARA"
BASE COUNT          978 a         2547 c         2312 g         1108 t
ORIGIN      
        1 cgcccagccg ccacgcgtcc accacggcga accagcgcaa cgccagcaac gcgagatccg
       61 cgacaaggac cgccgcgagg gcgttccgcg taaaggcggc gtccagcagc gcgtcgcgct
      121 ctgcagcgac catcgcagcg agcgccgcca gcgggacgac cgcaagcacg agcagtacga
      181 cgccgcggcg gatcgcacct gcgtaggcct gaccggcgcc cggcgcaagt gccgagagcg
      241 ccccggcgac gatccggtta ccgctgtcgg ccacgtctcg agtgtacgag ccggtggaca
      301 cgtcccctcc cttggcgtgc agtgtgcgac ggcgtaggct cggactcaac cttcgactga
      361 gacggagacg agatgtccgg ctacaccatc aagccgctgt ccgaagtccc cgacgtcctc
      421 ggtgactacc cgggcgagat gcagatgatc accaaggaga tcggcgccga gcagtcagct
      481 ctgacgtacc gcagaatgcc gccgaagacg ggcggcaaag gctcgtacgg gcaccggcac
      541 aagacgcagg aggagctgta cttcgtgatc tccggagcgc tccaattcaa gctcggcgac
      601 gacgtcgtcg acgttcccgc gcataccgcc gtccgggtgg cgcctcaggt ctggcgctcg
      661 gtctggaacg acgggcccgg cgacgccgag ctcttcatcg tctcgccccg cgtcgacgac
      721 cctcgtgcgg atacggagtc gatcgaggat ttctggcccg aagactgagc gcgccggcaa
      781 tcgctactcc gcagggttcc gctcgaggaa ggcgcatggg cggacgtcga gcgggagcgg
      841 cgactcgccg atgcgggtca cgcgcagcaa gccgtcggct tccgggatcg tgagctcgcc
      901 gccgacgtcg ggcagcggcc ccgcgacctc gagcagacgg tagccgtcgc cggtcggggc
      961 aaaggccacg aaaccctcga cgtaccttga gttcgccggc ggcgattcgc gctgctcgat
     1021 cctgtcgtcc gcctgctcga tcgccaggcc cagacgctcg atcgagcgcc ggagcgcgtt
     1081 gccggcatcg acgtacgcct cttcgctctc ggccaggcga gcgccgagcg aggcgagacc
     1141 cttgcgcagt gccttctcgg tcacgcggac ctgctcctgg gagacggccg actgggcgtc
     1201 gagccgctcg gcaagctcct cgacgacgtg ccggagctcg tctgtctcgt tgcgcgcgag
     1261 aacctcctcg acgcgctcga tccgccagcc gaacgacgag gagaggcgtt cgagctcctc
     1321 ttgaagctcc tcgcgagcgt cgaccgatgc agcatcgcgc tcctcggccg ccgacgcgag
     1381 tgcgtcgatg gactcggcct gcgcccgtgc gtcgtcggcc tgagcctcga gcgcctgccg
     1441 ctgagccgcc agctcccgac gaagcgcctg ctccacgact gcgctccgtt ccgctgaacg
     1501 acgcgctccg tcgtcgattc ggctttcgat ctcgccgacg gtcgtgctga gagtctcgtg
     1561 tgtctcggcg acgccgctcg cgagctcgtc cttggtcgcg gcgagctcgg acttgagtgc
     1621 ctcgacggcc tcgccggctc ggtgtgcgac ctcgtctaca cgcctcgatg cctgcagcgc
     1681 ctctgtcgcg cccgacgtga tccgctcgct cagctcgtcg aggcggcgtt ccgtgtcctg
     1741 gacgaacgat gcgaactcgt cccgcacgtc ggcttccgcg atctgagcct cgagcgtctc
     1801 gtggtgtgcg gtgtgccgct cgagtgcggc ggcggcctgt cgcgcggtct cgtcgacggc
     1861 gcgcgccgcg gctgaggcga gctccgcgcc gaccgagtcc cgcagctcct cgaggcggtt
     1921 ccggagctcg tcggtgtcgc gtgaggagct ggtcgcgagc cgagcgatcc ggtcttccag
     1981 ctccgcgagc ctgtctgagc cgacgagtcc cttttcggct tcggcaagtc gctcgtcgac
     2041 ggcagcgaga cgtgcacgaa gcggctcgac gccgccgagc cgctcgtcgg catcgtcggc
     2101 acgttctcgc agcacttcga tctcgtcgag ccgagcggcg gtctccttct ggcccgcgcg
     2161 cagggcggcg tactcggcaa gaagctcgtc tctcgtctcg cgtacgagtc gtgagagtga
     2221 atcgccgagt cgcttctcgg cttcgccgcc ttcctgcaga cgcaactctg cggcggccac
     2281 tcgatccgac agcgcgcgca gcgcccgcgt ctcctcgccg agcatgtcga tttgctcgct
     2341 cagctcgatc tgccgcgcct ggagctcggc ctgctggtcg cggagctcgg tctggccacc
     2401 ctggagctcg ttcaccgact ggatgacgcg accgctcagc gaagcctgca gacgaggctc
     2461 gataccttcg ctctcggcca gccggcgttc ggcctgggcc acccgttcgg aaagatcgtt
     2521 cgaggcgctc tcggcggctg ccgcgaggtt ttccattcgt tcgcgcagct cggtctgctc
     2581 ggcccgaaga gtggcctgct cctcctgcac cgagctcgcc gactcgacca ccaatccact
     2641 caacgactcg cgtagccgcg actcgagctc ttcgccctcg gccaggcgtc gctcgagatc
     2701 agccagccgg cccgcgacgc tgtcgaccga ggacagctgg ttcagggcct ggcccagggc
     2761 ctgacgcaac gcagcctggt cggcttgaat ggcatcggct gcctcctcga gaggcgccac
     2821 catgccgcgc agccgcccct cgacctccgc gccttcctcg agcctgcgct cgagctcttc
     2881 cacacgtgga tcgcgcatct cccctgcgtc cagtcggcct tgcagcgcgt cgactcgccc
     2941 ggtcagctca gcggccgccg cgatctctgc ggcgagctcg tcgacgcgat tcgtgagtgc
     3001 aatgcgcgcg gcgtgggcgg cctcggccga ttcctgtatc aaccccgtca gggacgagcg
     3061 aagctcgtcg tcgccctggc gggcgcgagt cgtcgcctcc tcgccgagcg cctcgagcga
     3121 ccccgtcaac tgctgctcga gctcggcggc ggctgccagg cgatcgtcca tctcggccag
     3181 ccggcgctcg acctcgtcaa tccggccacg ctctcggtcg ccgttctcga tccgggcacg
     3241 gagctcttct tgttcctcgc gcagcgcagc tgccgccgcg tcgatagtgg cctcgagccc
     3301 acgggcgcgc tgctcgaact cggcggcgcc gccgtcttgc tccagcttct gcagccgctc
     3361 acccatcgcg tcgagcgcac cgcgcagcgt cgacgactcg tctagccgtg ttcgcagctc
     3421 acgcagctcc gcgtgaacgg gctcagttgc gtcgcggacg acaggccgaa gctccgcgtg
     3481 gacgtcgatc tccgcacggt tgcttacctc gagcttccgc tccagctcgc cgatccgacg
     3541 ctgcaggctc tcctcgactt ccctgcgacc cgacgcaacc tcctcgagcg tcgtgcgcag
     3601 ctgctcgacg tcaccaaccc tgagctgcga ggctcccgcc agctcggaag caagctcgtc
     3661 gacgcgctcc tcgagccgcg cgagtgcgtt gccgccgagc gcgacctcct cgactcgggc
     3721 ggtcagcgct tcgacctggg actcgaaggt gctcggaatc gaatcgacgc gctcggacag
     3781 cgcgcgcatc tcggcgccga tggcgtcggc gtcgccgagg cgggcttcga gtagagagat
     3841 gctggtgcgg agcgcctcaa ggtcggaagc gccgggaaca gacgactcag gcgtccgagt
     3901 cgccagtgcc tccagctgcg tctggagctc gccgagcttc gcggggaggg tgtccgtctg
     3961 cgctattcgc tcggaaaagt cctcgagccg agattcgagc gacccgggca aggcctcgag
     4021 ctgatcggcg aggccggcga cctctgcgtg cacccgcgct gcggccgcga cccgctcgtc
     4081 caggccggcg atcgcagagc gaatctcttc cagatcggct gaggcggcgg tcggcgcgct
     4141 gctctgagcc tccacgagcg cctcgatctg ctgcgtcacg gagtccaggt ctgcacgcgc
     4201 ggccgtggtc tcggccgccc gcgcgacgat ctcttcgatc cgtgcctcga aggaggtcgg
     4261 gatggagtcg acacgctccg caagcgccgc cagctcggct cgcagctggg tgctttcgcc
     4321 gatgcgctgc tcgagcgacg agagctctgt tcgcagctcc tcgatcgacc cgctggagga
     4381 ctcgatgctc gccatccggc tccgcaactg atccacctcg acgtgcagcc cgtcggtcgc
     4441 accggcgagg gcactcgaaa ctcggtcgtc gagcgcggat gcagacgccg cggcaatctg
     4501 cagccgacct tccacggcgt cgagccgggt gacgagctcg gcatcgtcga actggggcac
     4561 ggcggcgatc cgggcggcaa gaccgtcgat ccgcgcacgc agccgctcga agtcgtccgt
     4621 ctcgccggtc ttctgctcga gcgcaacgag ccgctctcgc aagggctcga ggtcgaagtc
     4681 ggcagctgcg ccgtcgggcg gcgggcctag ggtggcgatc cgctcgcgaa gctccacgag
     4741 ctccccgcgg agctcgtcga ggacgccgga atccatgtgc gaacgctcga gggccgcgag
     4801 gcgctcctcg atctgggcaa gatcgatgtc ggctcggtcg tcccgctcca gccgcgccgc
     4861 gatggcatcg atgtcggcgc gcagcggcgc cagcgcctcg ccggttcccg cctcggaagc
     4921 tgcgggggaa ggctcagacg tgccgctcgc gtcctgcgcg gggcccggct cgccctggcc
     4981 ctcacgctcg gaagccggcg gacgaagctt ctgcagcgtc gaccactgat ctgcgagagc
     5041 gctcgccagc tcgagccgcg acacgactcc cttcgcgacc aggatctcgc cgagtcgctg
     5101 tccgctcttc gcctgtgccg cgagcgcctc ttcgagctgc tcttcggtca gcagaccgcg
     5161 ctcgcggaag atctccccga tgggctgccg cgcgatcatc tcctccggca cggcaggtgt
     5221 cgtgcccggc cactccgctg agagggccgc cagatcgctc ttgtcaatct ccggcgcgct
     5281 tccgcccgtc cgcggccgct cgtactcggc ccactgctcg gccagtgcgc tggcgagggc
     5341 caaccgcgag acgccgaact tgctgacgag gatctctccc acgcgctccc ccgaggcacg
     5401 ctgctgctcg agcgcctcgc tgagctgctc ctcggtgatg agccccttct cgacgaagat
     5461 tgcgccgatc aggcgcgacg acacagccgg agtgtccacg ggttgtctat cggcaatccc
     5521 tcgtccgagg tagagaggcg atttccggcc gcccaaaatg catgcacaca agccagcacc
     5581 ggatccgaag tactcaaaat cttgcggcta taggcgaaat acgctacgtt ttggatccga
     5641 actgcaattg gttcggatcc agcattgacg ccgggaggcc cgcatgactc atacgatcca
     5701 gatcgagtgc gcacaggccg tcgacggcga cgagctcctc gccgccttcg cagcgcaggg
     5761 cctccccggc gagatcgtcg gccgcgagcc ccggctcgtc ttccggctcg gcgacgctgg
     5821 acgcgacgcg cgccgctttg ccttcgacgt cgcccacgcc ctcgacgggt gggtcgccga
     5881 gcggcatgtc gcactcgtgc cgatgtcgat cggcgacgac gcgttcgtgc taaggccgcc
     5941 cgccgcctag acgaaccagt accgtcgtcg ggtgcaggtc gcgcggatcg cacccggcct
     6001 ctggcgctgg accgcgcgcc acccgtcttg gacgccggcc gacggagggc ctgacgggtg
     6061 ggaagcggag gttgcgtgtg tctactggga ggcgcccgac gccgtcgtac tcgtcgaccc
     6121 cctcgtaccc gcggagccgc cggaccggga gttgttctgg caggcgctcg accgcgacgt
     6181 cgaccggctg ggtctcccgg tggtcatcgt cctcacatgc gcgtggcacg cgcgaagcac
     6241 ggaggagatc ctcagccgct acgaggcgcg actgcgcgag gggagcgagc ccctgccggg
     6301 tacggccatc ggcttcccgg caggcgaaga gacgatcctg tgggtgcccg atgcgggtgc
     6361 actcgtcatc ggtgacgtgc tcgtcggtaa ccggtcggga ggagtccgac tctgccctgc
     6421 gtcatggctc cacggcggca cgccgaccgc cgacgtggcg gcgtcgctcc gcccgctcct
     6481 cgacctcccg gtcgagcaca ttctcgtctc ccacggcgac ccggtgctcg ggggaggcgc
     6541 cacggcactt cgacgagcgc tgagcgacgc cttctgaagg ctacgcgcgt gcgcccgccc
     6601 actgcgccag cgcatcaacc acgcggcgca gccgccggcc ttccggcgtg agccggtact
     6661 cgacccttgg gggccgcgca tcgatcacct cgcgctcgag cacgcccgcg gcctccagct
     6721 cgaccagacg ctgcgcgagc gtccgcggcg ggatccgtcc caccgcctgc cggaactcgt
     6781 tgaagcggct cgcgccctcg taggaggcgt agaggatcga gaccgtccag cgccgctcga
     6841 ggaggcccgc cgctcgacgg accacctccg gtaccggagc gcagtgctta cagcgcggcc
     6901 tcgagcgcgg cgctgcgggg cgcgagcgcg acgtcgagca ccgcg
//
LOCUS       DBNGNK010000282         9659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1840954,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000282 DBNGNK010000000
VERSION     DBNGNK010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1840954"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..776
                     /locus_tag="AC150V_08220"
     CDS             99..776
                     /locus_tag="AC150V_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR3762945.1"
                     /translation="MATVLVVDDEPIVREVVVRYLEREGYSTLEADDGDQARELLERR
                     DPDLVVLDVMLPGTDGLELCRWIRSRSELPVILLTARGEEADRIVGLELGADDYVTKP
                     FSPRELAARVRTVLRRAAPNGDGGERISFGDVELDASTRDVRKNGSELKLTAKEFDLL
                     WFLARNPRRVFSRDQLMSRVWGYEAALDTGTVTVHVRRLREKIEDEPSAPRHLQTVWG
                     VGYRLVP"
     gene            776..1840
                     /locus_tag="AC150V_08225"
     CDS             776..1840
                     /locus_tag="AC150V_08225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR3762946.1"
                     /translation="MVFSALVIAGATLGVGLLVSMLLRALPTVRLQVVGLAVLAVVLP
                     LVAVLLSGWVMFHMGDDVKILAVSAASAAIAVVAGLFLAGSISRSVDRVRDAATELAG
                     GDLRARAPEDGPAEVAELAQSFNTMAENVERLFDARRELVAWASHDLRTPLANMQAML
                     EALEDGLAEPDDYLPALGEQVTALSVLVDDLFELARIDAGALTLELHSVCLAGVVDSC
                     LRGVEAEARLRSISLDSDIDENITARAAPEKVERVLLNLLTNALRHTPSDGAVAVHAQ
                     ALAHEVRVTVEDTGDGLDGEAQQRMFERFWRSDRARSSRGAGLGLAIARGLVEAQGGR
                     IWAEARPEGGASVSFTLPAA"
     gene            1942..2655
                     /locus_tag="AC150V_08230"
     CDS             1942..2655
                     /locus_tag="AC150V_08230"
                     /inference="COORDINATES: protein motif:HMM:NF038123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spondin domain-containing protein"
                     /protein_id="HIR3762947.1"
                     /translation="MKRLVLVLAAAALVGVAAAGSALAFGGAGHGPTLRWEVKITNLT
                     PATGPGTAQPLSPPSVTVHNRRADVWQVGATASDGVAAIAEDADNSVLEGALSGAPGV
                     KSVSTAPTGGPILPGASATFYVESDFWHNRISIVSMLVNTNDAFTGLDSKPLLPFGLF
                     GRTWYVRAYDAGSEQNDQLAANIPGPCCGDTGKNGTPTAEPIAHHPGILDAVGDLDPS
                     IHGWPGAVAKIEIRPAFHR"
     gene            complement(2716..3138)
                     /locus_tag="AC150V_08235"
     CDS             complement(2716..3138)
                     /locus_tag="AC150V_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3762948.1"
                     /translation="MVTRKLNALPLQEVWNESGMRLRVDFPLTRESGTESCAVVYFEI
                     EPDDHLARHTDSAEELLLVLEGEGIAVVGDERSPIGPGSLAVVPALVPHEVHATGDST
                     LKVIGFFAGAVIDHVFDEPLQPIGLAELRTPAPAGVAA"
     gene            3202..3756
                     /locus_tag="AC150V_08240"
     CDS             3202..3756
                     /locus_tag="AC150V_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3762949.1"
                     /translation="MTRGYQQRVRAERAAETRRVIVEATVALHEELGPAQTTVAAIAE
                     RAGVSRPTVYKQFPDETALFAACSAQWNEEHPAPELPGTDLEDTLLRLYRYYAENVRM
                     IEGVERDALLEPPVAQTIKPFWDALDQVACEHAGDVGGTPATRAALRLVLDFWTWRRL
                     AREGLSEEQAAALAAGLVRCVGRR"
     gene            complement(3753..4238)
                     /gene="ispF"
                     /locus_tag="AC150V_08245"
     CDS             complement(3753..4238)
                     /gene="ispF"
                     /locus_tag="AC150V_08245"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015778861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR3762950.1"
                     /translation="MTDLRIGLGVDAHALVDGVPLVLGGVRIDDPRGLTGHSDGDVIA
                     HALTDALLGAAGLADIGAYFPSDDERYRGADSLELLAHAYVSVREAGYELVSADCMLI
                     GEEPRIAPHREDMRRRLADALGVDPERVNVRATTTDRLGFTGRGEGLAAQAVALVERR
                     R"
     gene            complement(4248..4928)
                     /gene="ispD"
                     /locus_tag="AC150V_08250"
     CDS             complement(4248..4928)
                     /gene="ispD"
                     /locus_tag="AC150V_08250"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013125282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR3762951.1"
                     /translation="MSTWALLVAAGAGERLGGDRPKAFVGLSGRPLLAEATERLDRTD
                     WVDRIVIASAAGWEEPAMLLAEELAANKVVAVVTGGSSRAESVRAALEQVPDEALVVL
                     VHDAARPLLGEDVIERVLAPLAEGFDGAVPGLPVADTVKRVDRSGFVRETLDRSELVT
                     VQTPQAFTAPALRRAFAGDLSDATDCASLVERAGGRIRVVDGDPRLIKITTPADLAHV
                     ERLLRDDG"
     gene            complement(4935..5495)
                     /locus_tag="AC150V_08255"
     CDS             complement(4935..5495)
                     /locus_tag="AC150V_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR3762952.1"
                     /translation="MSHSIQGRQSLFDVGDKVVYPHHGAGTVVKIESRKVLGEERDYL
                     TIKILHNEMTVNVPCENAERVGLRGVIDQQMVDVVVKALSGGSTEMPKNWNRRFKHNR
                     DKMKTGDILELAEVVRNLSLRDHEKGLSTGEKQMFVKAKKILASELMYAKDMSEDEAA
                     DWLDEVLSKPARKSSKSKAKVAAASA"
     gene            complement(5667..7031)
                     /gene="radA"
                     /locus_tag="AC150V_08260"
     CDS             complement(5667..7031)
                     /gene="radA"
                     /locus_tag="AC150V_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR3762953.1"
                     /translation="MGKVVVQHVCSDCGTTTGRWLGRCPGCGSFGTLVEELTGRETAT
                     AARPLQRLGDVESADAARISTGVPELDRVLGGGLVPSSLVLVGGEPGVGKSTLLLSAL
                     AAIAGAGRRALLVTGEESPAQVKLRAERLGGAEAVEILAETELEGVCATLERERPGVC
                     VIDSVQTLYSGELGSAPGSVAQVRESAARLLRVAKEFGVATILVGHVTKDGAVAGPRV
                     LEHLVDCVLQFEGDRYHAHRILRAVKNRFGSTNELGVFEMTGAGLVGVPDPSELFGKT
                     TPGEVGAAVACALEGTRPLLLEIQALVSKSELAMPRRVGTGVDPKRLAMIVAVLARHA
                     RVALGSADVFVNVAGGVHIDEPGADLAIALAIASAHRGTPVQDGLAAFGEIGLTGRLR
                     PATQVDRRLEECAKLGLAVVVAPAGCSGNGRPRVQSAATLREAIRVGLRQNSDGDSEV
                     DAED"
     gene            complement(7108..9039)
                     /locus_tag="AC150V_08265"
     CDS             complement(7108..9039)
                     /locus_tag="AC150V_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR3762954.1"
                     /translation="MPPDPFVSSAQRSPWPRRIAIAASGVAALLLLAAAALGGLAWHD
                     RGRIPQNTTVAGVDVGGLTVEQAGRQVRAAANARLDQPISIVAPGTETEVSGALLGAT
                     PQLEAALRAAKDAGFVDRLQRRFGFGKTRIVPLRYRPDPVRVEALYEELAARFDKKPR
                     SAKLVVTGSGVTVVPAVLGTEVDQAALSRGLRRLPTTLGVPLVDVDPPLTTAEAMRAK
                     QKADRLLAEPRAVRFPEGEAVLRAPALRRALRFETKGNGTVSLGLNPDSLRASLAKRL
                     RKVESEPVDAVFRVEGERVRIVPSKPGRTFDAAALAASLTGNLGSTVHRARFAVSQPP
                     LTTAKAKKLRIRELVSEFTTFYNCCQPRVTNIQRAAQLLDGTIIRPREVFSMNEALGR
                     RTTENGFVSAPQIFRGRLEDAIGGGISQVATTLYNAAFFSGVKLVEHQAHQFYISRYP
                     MGREATVSWGGPELIFRNDWPAAILMKVDASDSGIRVRFYSAKLGRRVETVTGEPYDY
                     RSPATIQIANSTLIKGTTNVLQSAGASGFTVQYTRKVFKGPKLVKNERYTVRYDPQNA
                     YVEVGTKPKPKPPKPKQGKKKGTDAEPSRPGAGEAPVPGPQAPVSGPETPPDGAGESA
                     LEADGATAETGGPSEPSSS"
     gene            complement(9088..9540)
                     /locus_tag="AC150V_08270"
     CDS             complement(9088..9540)
                     /locus_tag="AC150V_08270"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3762955.1"
                     /translation="MAVSIRHAQPSDYGRVIQHLNAWWGGREIAPMLPKLFFVHFEGT
                     SLVAETDEGVLVGFLVGFLSQTHSDQAYVHFVGVAPEFRGRGVGRSLYEAFFGAARVH
                     GRTVVRCVTSPTNTDSVAFHEALGFEVDSVVEDYDMQGETRALLSKRL"
BASE COUNT         1505 a         3366 c         3257 g         1531 t
ORIGIN      
        1 caggcactcg aacgcgacgg cctggtcgtc gagacggacg gcgtcgtctc gcttccgcgt
       61 taacccctcc gtaaggcatc gtcgcgcaca atgcgggggt ggcaaccgtt ctcgtcgtag
      121 acgacgagcc gatcgtccgt gaagttgtcg tccgctatct ggagcgggag ggctactcca
      181 ccctcgaggc ggacgacggc gaccaggcac gcgagctgct cgagcgccgt gaccctgatc
      241 tcgtcgtcct ggacgtcatg ctgccgggga cggacgggct cgagctctgt cgctggatcc
      301 gctcgcgctc cgagctgccg gtgatcctcc tcaccgcccg gggagaggag gcagaccgga
      361 tcgtcgggct cgagctcggc gccgacgact acgtgaccaa gccgttttcg ccccgagagc
      421 tcgcagcccg cgtgcggacc gtcctgcgcc gcgcggctcc caacggcgac gggggtgagc
      481 ggatctcgtt cggcgacgtg gagctggacg catcgacccg tgacgttcgg aagaacggct
      541 cggagctgaa gctgacggcc aaggaattcg atcttctctg gttcctggcg cgcaacccgc
      601 ggcgggtgtt ctcgcgcgac cagctgatga gccgagtctg gggctacgag gctgcgctcg
      661 acacaggcac tgtcacggtt cacgtccgcc gtctgcggga gaagatcgag gacgagccct
      721 ccgcgccccg ccacctgcag accgtatggg gcgtcggcta tcggctggtt ccctgatggt
      781 cttctccgcg ctcgtcatcg caggcgccac cctcggcgtc ggccttctcg tctcgatgct
      841 gctgcgggcg ctccctaccg tgcggctgca ggtcgtgggt ctcgccgtgc tggcggtcgt
      901 gctaccgctc gtcgccgtcc tgctctcggg ctgggtgatg ttccacatgg gcgacgacgt
      961 caagatcctc gccgtgtcgg cggcatcggc cgccatcgca gtcgtggccg gcctcttcct
     1021 tgccggctcc atctcgcgct cggtcgaccg ggtgcgcgac gcggccaccg agctcgccgg
     1081 cggtgacctg cgcgcccgcg ccccagagga cgggcccgcc gaggtcgccg agctcgccca
     1141 gtcgttcaac acgatggccg agaacgtcga gcgtctcttc gacgcgaggc gagagctcgt
     1201 tgcgtgggcg agtcacgatc tgcgcacgcc gctggcgaac atgcaggcga tgctcgaagc
     1261 gctcgaggac ggcctcgccg agcccgacga ctacctgccg gccctcgggg agcaggtcac
     1321 agctctctcg gtgctggtcg acgacctgtt cgagctcgcc cgcatcgacg ccggtgcatt
     1381 gacgctggag ctccactccg tctgcttggc gggcgtcgtc gactcctgcc tgcgcggcgt
     1441 cgaggcggaa gcgagactcc ggtcgatctc gctcgactct gacatcgacg agaacatcac
     1501 cgcacgggcc gccccggaga aggtcgagcg cgtgctgctc aacctgctca cgaatgcgct
     1561 gcgccacacg ccgtccgacg gcgccgtggc tgttcacgcc caggccctgg cccacgaggt
     1621 gcgcgtgacc gtcgaggaca ccggtgacgg gctcgacggc gaggcgcagc agcggatgtt
     1681 cgagcgcttc tggcgcagcg accgggcgcg ttcgagccga ggcgccgggc tcggcctcgc
     1741 cattgcccgc ggcctcgtcg aggcgcaggg cgggcgtatc tgggccgagg ctcgacccga
     1801 gggcggcgcc agcgtgagct tcacgctccc tgctgcttga cgggcggcgg caaagttcag
     1861 gatctcttga ggaggcccta agggcagcgc aagctggcgc tcctagcctc gagctgtcaa
     1921 ttcgaggcaa aggaggaatc ggtgaaacga ctcgtcttgg tgctcgcggc tgccgcgctc
     1981 gtgggagtcg ccgccgcagg atcggcgctc gccttcggcg gagctggaca tggtccgacg
     2041 ctccggtggg aggtgaagat cacgaacctc actccggcga cggggcccgg cacggctcag
     2101 ccgctatccc ctccgtccgt cacggtccac aatcgacggg ctgacgtgtg gcaggttggt
     2161 gcgacggcca gtgacggcgt ggccgcgatt gccgaggacg ccgacaacag cgttctcgaa
     2221 ggtgcgttgt cgggtgcacc aggcgtgaag agcgtctcga cggcgccaac cggtggaccg
     2281 attctccccg gagcgagcgc gactttctac gtcgagtcgg acttctggca caaccgcatc
     2341 tcaatcgtct cgatgcttgt caacacgaac gacgcgttca ccggcctcga ctcgaaacct
     2401 ctcctgccct tcggtctctt cggtcggaca tggtacgtcc gggcctacga cgccgggagc
     2461 gagcagaacg accagctcgc tgcgaacatc cccggcccct gctgcgggga cacggggaag
     2521 aacggaacgc cgaccgcaga gcccatcgca caccacccgg gcatcctcga cgctgtcgga
     2581 gacctcgacc ccagcatcca cggctggccg ggtgcggtcg cgaagatcga gatccgcccg
     2641 gccttccacc gctgagcagg cctctctcgc agaatgcgcg ggcccggacg tcgggcccgc
     2701 gcgggtgctg cgaactcagg cggccactcc ggcaggcgcg ggcgtcctga gctcggccag
     2761 gccgatcggc tgcaacggct cgtcgaagac gtggtcgatg acggccccgg cgaagaagcc
     2821 gatgaccttc agcgtgctgt caccggtcgc atggacttcg tgggggacga gagcgggaac
     2881 gaccgcgaga gagccgggcc cgatcgggga ccgctcgtcg ccgacgacgg cgatgccttc
     2941 gccctcgaga accagcaaga gctcctcggc gctgtcggtg tggcgggcga gatggtcgtc
     3001 cggctcgatc tcgaagtaga cgacggcgca gctctccgta ccgctttctc gggtaagcgg
     3061 gaagtcgacg cgcagccgca ttcccgactc gttccagact tcttgcagcg gtagagcgtt
     3121 cagctttcgg gtgaccatcg ttcctccttt tcgcttgaca aggcgtcact ctaaattttg
     3181 ggatacgcta tgtcaagcga aatgacacgc gggtatcagc aacgtgttcg agccgagcgt
     3241 gcggccgaga cgcggcgagt catcgtggag gcgacggtgg cgctccacga ggagctcggc
     3301 ccggcgcaaa cgacggtcgc ggcaatcgca gagagggccg gcgtctcccg accgaccgtc
     3361 tacaagcagt tccccgacga gacggcgctc ttcgccgcct gcagcgcgca gtggaacgag
     3421 gagcatccgg ctcccgagct acccggcacc gatctcgagg acacgctgct gcgcctgtat
     3481 cgatactacg ccgagaacgt ccgcatgatc gaaggggtgg aacgcgacgc tctcctcgaa
     3541 ccccctgtcg cgcagacgat caagccgttc tgggacgctc tcgaccaggt tgcctgcgaa
     3601 cacgccgggg acgtcggcgg cactcccgcg acgcgcgccg cgctccgcct cgtcctcgac
     3661 ttctggacgt ggaggcgact cgctcgagaa gggttgagcg aagagcaggc agcggccctc
     3721 gccgccggcc tcgtacgctg tgtcggaagg cgctagcgcc ggcgctcgac cagggcaaca
     3781 gcctgcgcag caagcccttc cccgcgcccg gtgaacccga gccgatcggt cgtggtcgcc
     3841 cgcacgttga cccgctcggg atcgacgccg agagcgtccg ccaggcgacg gcgcatgtcc
     3901 tctcggtgag gcgcgatgcg cggctcctcg ccgatcagca tgcagtccgc gctgaccagc
     3961 tcgtagccgg cctcacgcac gctcacgtac gcgtgcgcga gcaactcgag cgagtctgcc
     4021 ccgcggtagc gctcgtcatc ggaggggaag taggccccga tgtcggcgag ccccgcggcg
     4081 ccgagcaggg cgtcggtgag ggcgtgtgcg atcacgtcgc cgtcggagtg gcccgtaagg
     4141 ccacgaggat cgtctatccg cacgcccccg aggacgagcg gaaccccgtc gacgagcgcg
     4201 tgtgcgtcga cgcccagccc gatgcggaga tccgtcatgt ccggaagcta gccgtcatcg
     4261 cggagcagcc gctcgacgtg tgccaagtcg gcgggagtcg tgatcttgat cagcctcgga
     4321 tcaccgtcga cgacacggat ccggccgccc gcgcgctcca ccaacgacgc gcagtcggtg
     4381 gcgtcgctca gatcgccggc gaaggcacgg cgcaaggccg gcgccgtgaa cgcctgtggt
     4441 gtctgcaccg tcacgagctc gcttcggtcg agcgtctcgc ggacgaaccc gctccgatcg
     4501 acccgcttca cggtgtcggc aacgggaagc ccaggaacgg ccccatcgaa gccctctgcg
     4561 agcggggcga gcacacgctc gatgacgtcc tctcccagga gggggcgtgc agcatcgtga
     4621 acgagcacca cgagcgcctc gtccggcacc tgctcgagag ccgcgcgtac cgactccgct
     4681 ctcgagctgc cgccggtcac gacggcgacc accttgttcg ccgcgagctc ttccgcgagg
     4741 agcatcgcgg gctcctccca gcccgctgca gaggcgatca cgattcgatc gacccagtcg
     4801 gtccggtcga ggcgctccgt ggcctcggcc agcagcggcc gacccgacag cccgacgaac
     4861 gccttcggcc ggtccccgcc gagccgctcc cccgcgccgg cagcgacgag gagcgcccag
     4921 gtcgacaccc ccggctaggc gcttgcggcg gcgaccttgg ccttggactt cgaggacttt
     4981 cgagcgggct tcgaaagcac ctcgtcgagc cagtccgcag cctcgtcctc gctcatgtcc
     5041 ttcgcgtaca tgagctccga cgcgaggatc ttcttcgcct tgacgaacat ctgcttctcg
     5101 ccggtagaga gccccttctc gtggtcgcgg agcgacaagt tccgtacgac ttcggcgagc
     5161 tccaggatgt ccccggtctt catcttgtcg cggttgtgct tgaaccgccg gttccagttc
     5221 ttcggcatct ccgtcgaacc cccagagaga gccttcacga cgacgtcgac catctgctgg
     5281 tcgatcaccc cgcgcaagcc cacgcgctca gcgttctcgc acggaacgtt gaccgtcatc
     5341 tcgttgtgaa ggatcttgat cgtcaggtag tcgcgctcct cccccaggac cttccgcgac
     5401 tcgatcttga ccaccgtgcc cgccccgtgg tggggataca ccactttgtc gccgacgtcg
     5461 aacaagctct gcctcccctg aatcgaatga ctcacgaaaa gcgccggcag agagtgctac
     5521 tgccggcgaa cgcaagccat tctagcggag attaggcaca aaaatcggct caactgcgcg
     5581 acaaacacgt atcgatttcg ctcgaagtcc ggccgagaac cgcccgtgca gctcagccgg
     5641 agggcgccgg ctcacgaatc gagtcgtcaa tcctccgcgt ccacttccga gtcgccgtcg
     5701 gagttctgtc tcagcccgac ccgtatcgct tcgcgcaacg tggccgccga ctgcacccgc
     5761 ggtcgtccgt tgccgctgca gccggcggga gcgacgacca ccgcgagacc gagcttcgcg
     5821 cactcctcga gccgtcggtc gacctgggtt gccgggcgaa gtctcccggt caatccgatt
     5881 tctccgaaag cggcaaggcc gtcttgcacg ggggtgccgc ggtgcgccga cgcgatcgcc
     5941 agcgcgatcg cgagatccgc gcccggctcg tcgatgtgga caccgcctgc gacgttgacg
     6001 aacacgtctg cggagccgag cgccacgcgc gcgtggcgag cgaggacggc aacgatcatc
     6061 gcgagccgct tcgggtcgac gcccgtaccc acgcgccgag gcatcgcgag ctcggacttc
     6121 gaaacaaggg cctgaatctc gagcagaagc ggccgggtcc cctcgagggc gcaggcaacg
     6181 gcggcgccga cctcgccggg ggtcgtcttc ccgaacagct ccgaggggtc ggggacgccg
     6241 acgagcccag ctcctgtcat ctcgaagacc ccgagctcgt tggtcgatcc gaagcgattc
     6301 ttcacagcgc gaaggattcg gtgcgcgtgg taccggtcgc cctcgaactg gagcacgcag
     6361 tcgacgaggt gctccagcac gcgcgggccc gcgaccgcac cgtccttcgt cacgtgcccg
     6421 acgagaatcg tcgccacacc gaactccttg gcgacgcgga gcaggcgagc cgcactttct
     6481 cggacctgcg ccaccgaacc gggcgccgac ccgagctcac ccgagtagag cgtctggacg
     6541 gagtcgatta cgcacacgcc ggggcgctcc cgctcgagcg tggcgcaaac cccttcgagc
     6601 tcggtctcgg cgaggatctc gacggcttcg gcgccgccca ggcgctcggc acggagcttg
     6661 acctgcgcgg gcgactcctc tcccgtgacg agaagcgcgc gccggcctgc gccggctatc
     6721 gcggccagag ccgagagcag cagcgtcgac ttgccgaccc cgggttcgcc gccgacgaga
     6781 acgagcgagg acggaaccag gccgcccccg aggactcggt cgagctcggg tacgccggtc
     6841 gagatcctgg cggcgtccgc ggactcgacg tcaccgagcc gttgcagcgg cctcgccgcg
     6901 gtggcggtct cgcgacctgt cagctcctcg acgagcgtcc cgaaggagcc gcagccgggg
     6961 cagcgcccga gccagcggcc ggtggtggta ccgcagtcgg agcacacgtg ctggacgacg
     7021 actttgccca cgatgccatc gtagactcga catcggacgt cgtggtgtca gtactgacaa
     7081 cgaaggggag ctgcgccgat cggcggctca tgacgaactc ggctcggacg ggccgccggt
     7141 ctcggccgtg gcgccgtctg cctccagggc gctctctccg gcgccgtcgg gcggtgtttc
     7201 gggaccggag accggggcct gcggaccggg tacaggcgcc tcgcccgcac cgggtcgact
     7261 cggctctgcg tcggtgcctt tcttctttcc ctgcttgggc ttcggcggct tgggcttcgg
     7321 cttggtgccg acctcgacgt aggcgttctg cgggtcgtag cggacggtgt agcgctcgtt
     7381 cttcacgagt ttcggcccct tgaagacctt gcgcgtgtac tgaacggtga agccggaggc
     7441 accggcgctc tgcaggacgt tcgtcgttcc cttgatcaac gtcgagttgg cgatctggat
     7501 tgtggccggc gagcggtagt cgtacggctc cccggtgacg gtctcgacgc ggcgcccgag
     7561 cttggcggag tagaaccgaa ccctgatgcc tgaatcactg gcgtcgactt tcatcagaat
     7621 cgccgccggc cagtcgttcc ggaagatgag ctcgggccca ccccacgaga cggtcgcctc
     7681 gcgccccatc gggtagcgcg agatgtagaa ctggtgcgcc tgatgctcca cgagtttcac
     7741 cccggagaag aaggctgcgt tgtagagcgt cgtcgcgact tggctgatac cgccgccgat
     7801 cgcgtcctcg agccgtccgc gaaagatctg cggcgccgag acgaacccgt tctccgtcgt
     7861 tcgccggccc agcgcctcgt tcatcgagaa gacctctctg ggccggatga tcgtaccgtc
     7921 gaggagctgc gccgcccgct ggatgttcgt cacgcgcggt tggcagcagt tgtagaacgt
     7981 ggtgaactcc gacaccagct cacgaatccg cagcttcttc gccttggcag tcgtgagtgg
     8041 cggctggctg accgcgaacc gggctcgatg gacggtcgag ccgaggttgc ccgtgagcga
     8101 cgctgcgagc gccgcggcgt cgaacgtgcg ccccggcttc gacgggacga tccgaacgcg
     8161 ctctccctcg actcggaaga cggcgtcgac cggctcgctc tcgaccttcc ggagtcgctt
     8221 ggccagcgat gcgcgcagtg agtccgggtt gaggccgagg gagacggttc cgttgccctt
     8281 cgtctcgaag cggagtgctc tgcgaagcgc cggagcacgg agaaccgcct cgccctcggg
     8341 aaagcgcacg gcgcggggct ccgcgagcaa ccgatcggcc ttctgtttcg ccctcatcgc
     8401 ctccgcggtt gtcagcggtg gatcgacgtc caccagaggc actccgagcg tggtcgggag
     8461 gcggcgcagg ccacgggaga gagcagcctg gtcgacctcc gtcccgagaa ccgctggtac
     8521 gaccgtgact ccagagcccg tgaccacgag ctttgcggag cggggcttct tgtcgaagcg
     8581 cgcggcgagc tcctcgtaca gcgcctcgac gcgaacggga tcgggccgat agcggagtgg
     8641 cacgatacgg gtcttcccga acccgaagcg tcgctgcagg cggtcgacga accccgcgtc
     8701 cttcgcggcc ctcagcgcag cttccagttg cggcgtcgca cccagcagcg cgccgctgac
     8761 ctcggtctcc gtgccggggg cgacgatcga gatcggctga tccaggcgcg cgttcgcagc
     8821 ggcccgcact tgcctgcccg cctgctccac cgtcagaccg ccgacatcca cacctgccac
     8881 cgtggtgttc tgcgggatac gcccgcggtc gtgccacgcc agaccgccca gcgcagcggc
     8941 ggccaagagg agcagcgccg cgacaccgct ggcggcgatc gcgattcgac gcggccaggg
     9001 cgatcgctgc gcgctcgaga cgaacggatc cggcggcatc cagccagcac ggtagcggac
     9061 ggatcagcgc ccgcgccggg ttcttcgcta cagccgtttg ctcagaagcg ccctggtctc
     9121 gccctgcatg tcgtagtcct cgacgacact gtcgacctcg aagccgagcg cctcgtggaa
     9181 ggcgaccgag tcggtgttcg tgggcgaggt cacgcagcgt acgaccgtcc gcccgtggac
     9241 tcgcgcggcg ccgaagaagg cttcgtagag ggagcgccct accccgcgac cccggaactc
     9301 cggcgccacc ccgacgaagt gcacgtacgc ctggtcgctg tgcgtctgcg acaggaagcc
     9361 gacgagaaag ccgacgagca ccccctcgtc ggtctcggcc acgaggctcg tgccctcgaa
     9421 gtggacgaag aagagcttcg gcagcatcgg cgcgatctcg cggccgcccc accaggcgtt
     9481 gaggtgctgg atcacgcgcc cgtagtcgga gggctgggcg tgacggatcg acacggccat
     9541 gcgggtggca cgctagccac cgcgcgatcg cgcgtctact gcgatcatca cgtgatgaga
     9601 ggtgtcgctc tgctcgcggt cgcgctgctc tgcggcttgg ggacaagcgc ttgcggggg
//
LOCUS       DBNGNK010000283         2500 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1265635,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000283 DBNGNK010000000
VERSION     DBNGNK010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1265635"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..493)
                     /locus_tag="AC150V_08275"
     CDS             complement(62..493)
                     /locus_tag="AC150V_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3762956.1"
                     /translation="MSGERVRDAMVAAPRVLDADATATDAGEALARPEVRAVIVCESD
                     GRLLGVLTRKTLVREIVAAGRDPNAVALREIAEPPHFTLDADEPVEDAFRALEEGDYE
                     RVPVIEGGRFVGVLSRSVLQRRLAEDEDPEDDEPPDLPPAA"
     gene            660..1667
                     /locus_tag="AC150V_08280"
     CDS             660..1667
                     /locus_tag="AC150V_08280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3762957.1"
                     /translation="MGYNDDRAVAITSNGRTRRPAAPDPYRRLPGIVFQLMREEEPEA
                     VRRVAEETLPLLVPHDVLRVSETSRDEILRALQGDGATAARAAQASPQEPANPVTLPL
                     IAHGVLVGELEVGRWSGESFGESERAVLGAFAELVALALHKAHAQHELARLAHTDPLT
                     GLANRRGLTRALEQSAPGSSLLLLDLDGLKAVNDDLGYDEGDLVICAAADALRAELRE
                     GELGARLGGDEFVALLFDEAEKRAARLNDTVDSLLLPPEVRERFRGGSVGLVAARPGE
                     GPDELLRRAAIRMKERKRLRKSVRDVPSAPDRDELGRAGAAGQEPVDFGPRPRGADTV
                     S"
     gene            complement(1660..2358)
                     /locus_tag="AC150V_08285"
     CDS             complement(1660..2358)
                     /locus_tag="AC150V_08285"
                     /inference="COORDINATES: protein motif:HMM:NF014373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR3762958.1"
                     /translation="MTLTAALAPQIPLWEDVVRLLVVSVLASVVGIERELRDQEAGLR
                     THMLVGVGAALFVITANFAWSDIGFGNAVGVVLDPSRVVAYVITGIGFLGAGTIIKHG
                     MNVKGLTTAASLWVVAAIGVTIGAGEYGLGLVATATVLLSLWPIRELARQVGLRADRT
                     HRLEIELAPEAKLESVIRSLERHGNRIASVRVSEEVSARRLEVVVVERKAELGQLVES
                     AGSLPEVRTASISN"
BASE COUNT          385 a          886 c          875 g          354 t
ORIGIN      
        1 ccgccgtcga ggcgagcgac cgtgacaccc tgcacggagc cgtgctcgcg gccgccgagc
       61 gctaggccgc gggcggcaga tccggcggct cgtcgtcctc cgggtcttcg tcctcggcga
      121 ggcggcgctg cagcacgctg cgcgagagca cgccgacgaa gcgcccgccc tcgatcaccg
      181 gaacacgctc gtagtcgcct tcttcgagcg cccggaatgc gtcttccacc ggctcgtcgg
      241 cgtccagggt gaagtgaggc ggctcggcga tctcccgcag cgcgacggcg ttcgggtcgc
      301 ggccggcggc gacgatctcg cgcacgagcg tcttacgggt gagcacaccg agcaagcgcc
      361 cgtccgactc gcacacgatc actgcccgca cctctgggcg ggcgagcgcc tctccggcgt
      421 cggtcgcggt cgcgtcggcg tcgagcacgc gcggcgcggc caccatcgcg tcccggactc
      481 gctccccgct catacccgcc tcctgccgtc cgtacggcgg cgcttcgcct cagaggcatg
      541 ggcattcatg gggagcggag tgtacaaccg cgcggcgcga aagacccctc ccggcatccc
      601 tcgatcgagg gatgccgcgc gctcgacgtg ggcgcaccat ggtggggaga gaagaccggg
      661 tgggatacaa cgacgacaga gcagtcgcga tcacgtccaa cggacggacg cgacgacccg
      721 cggcgccgga tccataccgg cgcctgccgg ggatcgtctt ccagctgatg cgcgaggagg
      781 agcccgaggc cgtgcgccgc gtcgccgagg agacgctgcc actcctcgtg ccgcacgacg
      841 ttcttcgggt gagcgagacg agccgggacg agatcctgcg tgcgctgcag ggcgacggcg
      901 cgacggcggc ccgagcagct caggcgtcgc cgcaggagcc ggcgaacccg gtgacgctgc
      961 cgctcatcgc ccacggcgtg ctggtcgggg agctcgaggt cggccgctgg agcggcgagt
     1021 ctttcggcga gagcgagcgc gccgttctcg gtgcattcgc cgagctggtg gcgctcgccc
     1081 tccacaaggc acacgctcag cacgagctcg cgcggcttgc ccacacggat cccctgaccg
     1141 gcctcgcgaa tcggcgtgga ctcacgcgcg cgctggagca gtcggcgccc gggagttcgc
     1201 tgctgctgct cgatctcgac ggcctgaaag ccgtcaacga cgatctcggc tatgacgagg
     1261 gcgacctcgt catctgtgcg gcggccgacg ccttgcgcgc cgagctgcgc gagggcgagc
     1321 tcggtgctcg cctcggcggc gacgagttcg tggcgcttct cttcgacgag gccgagaagc
     1381 gtgccgcccg gctgaacgac acagtcgact cgctgctctt gccgccggaa gtgcgagagc
     1441 ggttccgcgg gggcagcgtc ggcctcgtcg cggcccgacc cggcgagggg cccgacgagc
     1501 ttctgcggcg ggcggcgatc cgcatgaagg agcgcaagag attgcgcaag agcgtgcggg
     1561 acgtaccgtc tgctcccgat cgggacgagc tcgggagggc tggcgcggct ggtcaggagc
     1621 cggtcgactt cggcccgaga ccgagagggg cagacacggt cagttagaga tcgaggccgt
     1681 ccggacctct ggaagcgagc cggccgactc cacgagctga ccgagctccg ctttgcgttc
     1741 caccaccacg acctcgagcc tccgcgcaga cacctcctct gacacccgga ccgacgcaat
     1801 gcggtttccg tgccgctcga gcgagcggat cacgctttcg agcttcgcct cgggcgccag
     1861 ctcgatctcc agccgatgcg ttctatccgc acggagaccg acctgcctcg ccagctcacg
     1921 gatgggccag aggctgagga ggacggtcgc cgtggctacg agtccgagcc cgtactcgcc
     1981 tgccccgatg gtgacgccga tcgccgccac gacccagagt gaggctgccg tcgtcagccc
     2041 tttgacgttc atgccgtgct tgatgatcgt cccggcaccg agaaagccga ttccggtgat
     2101 cacgtacgcg acgacgcgcg acggatcgag caccactccg actgcgttcc cgaagccgat
     2161 gtccgaccag gcgaagttcg cggtgatcac gaacagcgcc gcgccgaccc cgacgagcat
     2221 gtgcgtgcgc agccccgcct cctggtcgcg gagctctcgc tcgatgccga caacgctcgc
     2281 gagcacggag accacgagaa ggcggacaac atcctcccat aacggaatct gtggcgcgag
     2341 ggcggctgtg agcgtcatgc aactcccttc taccccgttg tcgccgggtt caatccgcgg
     2401 acgggtgccg agagcgacgc gccagtgagc gctgctggcc gcgatcctca ggcggcgcag
     2461 cgtgagcgcg gcgagccgcc cttcagatgt agacgccagg
//
LOCUS       DBNGNK010000284         3262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_406934,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000284 DBNGNK010000000
VERSION     DBNGNK010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_406934"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..375)
                     /locus_tag="AC150V_08290"
     CDS             complement(28..375)
                     /locus_tag="AC150V_08290"
                     /inference="COORDINATES: protein motif:HMM:NF049942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DF family (seleno)protein"
                     /protein_id="HIR3762959.1"
                     /translation="MNSKANCRAVVEILTFEGCPNHEPAIELVERVSRELKLDPEIRL
                     VNVPDDEAAQRLRFLGSPTIRVDGRDVDCNTEERDDYSLSCRVFRTEAGLAGQPEERW
                     VRDALLRAATTGE"
     gene            complement(579..938)
                     /locus_tag="AC150V_08295"
     CDS             complement(579..938)
                     /locus_tag="AC150V_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR3762960.1"
                     /translation="MKLTATPFRLPETPDRADLVAKYFRGLGDPTRLRILELLRERGE
                     LTVGELVEQLAIAQPKVSNHLACLRWCGFVETRRDHRTVYNRIADDRVAELIELGNAL
                     LADNEEHVGACCRIERL"
     gene            1042..1497
                     /locus_tag="AC150V_08300"
     CDS             1042..1497
                     /locus_tag="AC150V_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HIR3762961.1"
                     /translation="MASAATETRLEPLFEAELVHRHDADPVVAPAEGDGAMIGSGDGI
                     VSGSDLRGGIRWTLFEHVGETLCAMNPFAVIETDDGATLNIEGRGWARRPQAGDSLWA
                     VAATLSFSTDDDRYLWLNDTLGHWEGEFDSSGGRAHYRAYARRRPEEAA"
     gene            1494..2198
                     /locus_tag="AC150V_08305"
     CDS             1494..2198
                     /locus_tag="AC150V_08305"
                     /inference="COORDINATES: protein motif:HMM:NF015216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylmercury lyase family protein"
                     /protein_id="HIR3762962.1"
                     /translation="MSGTSSDASRGSTEVVVGIAAERAGCQRTAGLTEPDRELYRFIL
                     RTFAVGVTPGGDELRREAVRLGVEPGTALDRLAEADLAHYDRSSHSIAIAYPFSGRPT
                     EHQIHLGERRDVYAMCAVDALGIPYMLDQPAEVLSRDSLDGSEIRVRIDPAGETTWEP
                     ESAVVVAGATGMSGPSAETCCQFMDFFSSEDNARRYLREHPELRGDVLGMRRAIDAGR
                     TIFGGLLDGDNGSEVC"
     gene            2195..2674
                     /locus_tag="AC150V_08310"
     CDS             2195..2674
                     /locus_tag="AC150V_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762963.1"
                     /translation="MSSPGTALTAGANRSIGPIGTALRVCVAVALLFLAVTDVGGDLT
                     WSIDWWQILLGLVGFPLLLTSLQRLRLRYTQAPLRETSHLGFCLNLVIGAALVLFPPT
                     RAATLLFLGASLLVAAWRGYAGCESLAISNWLLRREDTVGCVLFSPIDEGEKRLTGR"
     gene            2731..2868
                     /locus_tag="AC150V_08315"
     CDS             2731..2868
                     /locus_tag="AC150V_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762964.1"
                     /translation="MSADTIRSAYVALGEGDVEPLVALMDPEMGWHGYRGWRFWRPRP
                     S"
     gene            3178..3262
                     /locus_tag="AC150V_08320"
     tRNA            3178..3262
                     /locus_tag="AC150V_08320"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3212..3214,aa:Ser,seq:tga)
BASE COUNT          533 a         1083 c         1138 g          508 t
ORIGIN      
        1 cattggggtg cctggcggac tccttcgcta ctcgccggtc gtcgcggcgc gcaagagcgc
       61 gtcgcgtacc cagcgctcct ccggctggcc ggccaggccg gcctcggtcc ggaagacgcg
      121 acaggagagc gagtagtcgt cccgctcctc ggtgttgcag tccacatccc ggccgtcgac
      181 gcggatcgtg ggggagccga ggaagcgcag acgctgggcc gcctcgtcgt ccggcacgtt
      241 gacgaggcga atctcgggat cgagcttgag ctcccggctc acgcgctcga cgagctcgat
      301 tgccggttcg tggttcgggc agccctcgaa cgtcagaatc tcgacgactg cgcgacagtt
      361 ggccttgctg ttcaactaga cctcccttcg tgggcgaacg ggcggcggga gcggattcga
      421 aacacaagga cgagggcgac caccgcgagg gcgaaagttc caagcacaga actgcaggcg
      481 atcgtgagcg cgacggcgcc gagtccggcg gctgcgagag ggagtgcggc gcagcaccgc
      541 aaccgcggcc agagcgaccc cgcctgccgt ctcgcgcatc agagccgctc gatccggcag
      601 caggccccga cgtgctcctc gttgtcggcg agcagcgcgt taccgagctc gatcagctcg
      661 gcgacgcggt cgtcggcgat gcggttgtag acggtgcggt ggtcgcggcg ggtctcgacg
      721 aagccgcacc agcggaggca ggcgaggtgg ttggagacct ttggttgtgc gatcgcgagt
      781 tgctcgacga gctcgccgac ggtcagctcg ccgcgctcac gcagtagctc gaggatgcga
      841 aggcgggtcg ggtcgccgag gccgcggaag tacttggcga cgagatcggc gcggtccggc
      901 gtttcgggaa gtcggaaggg tgtcgcggtc agcttcaata tgtctccttt ttgatataag
      961 gataatccaa tattaagcaa tcccgcaagc gcggaagcgg cgcatcggcg aggacgccgg
     1021 ccgcgtccaa ggaggtaagg catggcatcc gccgcgaccg aaacaaggct cgagcccctc
     1081 ttcgaggctg agctcgtgca ccggcacgac gccgacccgg ttgtcgctcc cgccgaaggc
     1141 gacggcgcga tgatcggaag cggcgacggg atcgtttcgg gctccgacct gcgcgggggc
     1201 atccgctgga cgctgttcga gcacgtcggc gagacgctct gcgcgatgaa cccgttcgcg
     1261 gtcatcgaga ctgacgacgg cgccacactc aacatcgagg ggcgtggctg ggctcggcgc
     1321 ccgcaggccg gagacagcct ctgggccgtc gccgccactc tcagcttctc aaccgatgac
     1381 gatcgctacc tctggctcaa cgacacgctc ggccactggg aaggcgagtt cgactcctcg
     1441 ggaggccgag cccactaccg tgcctacgcg cgacgccgtc cagaggaggc cgcatgagcg
     1501 ggacttcgag cgacgcgagc cgtggatcga ccgaggtcgt cgtcgggatc gcggcagaac
     1561 gagccggctg ccagaggacc gccgggctga ccgagcctga tcgcgagctc taccgcttca
     1621 tcctgcggac gttcgcggtg ggtgtgactc cgggaggcga cgagctacgg cgcgaggcgg
     1681 tgaggctggg cgtcgagccc gggactgcgc tcgaccggct cgcggaggcg gacctcgccc
     1741 actacgaccg gagcagccac agcatcgcga tcgcctaccc gttctcgggg cggcccaccg
     1801 agcaccaaat ccacctcggc gagaggcgcg atgtctacgc catgtgcgcg gtcgacgcgc
     1861 tgggcatccc gtacatgctc gaccagcctg cggaagtgct ctcgcgggac tcactcgacg
     1921 gcagcgagat ccgcgttcgt atcgacccag cgggggagac gacgtgggag cccgagagcg
     1981 cggtcgtcgt cgccggcgca acgggaatga gtggcccgag cgccgagacc tgctgtcagt
     2041 tcatggactt cttcagctcc gaggacaacg cgaggcggta cctgcgcgag catcccgagc
     2101 ttcgaggaga cgttctcggg atgagacgcg cgatcgacgc cggccgcacg atcttcggcg
     2161 gcctgctcga cggcgacaac ggaagcgagg tctgttgagc tctcccggga cggcactgac
     2221 cgcgggtgcg aatcggagca tcgggccgat cggcaccgcg ctacgggtgt gcgttgccgt
     2281 agcgcttctc tttctcgcgg tcaccgacgt cggcggcgac ctgacgtgga gcatcgattg
     2341 gtggcagatc ctcctgggcc tcgtcggctt ccctctccta ctcacgtccc tgcagcggct
     2401 ccggctgcgc tacacacagg ctccgctccg ggagaccagt cacctcggat tctgtctcaa
     2461 cctcgtaatc ggagctgcgc tcgtcctctt tcccccgacg cgggcagcga cgctgctctt
     2521 tctcggcgcc tcgctgctcg tcgccgcgtg gcgaggctac gccggctgcg agagcctcgc
     2581 catctcgaac tggctcctcc gacgcgaaga caccgtcggc tgcgtcctct tctccccgat
     2641 cgacgaaggc gaaaagcgcc tgaccggccg gtaaggccac acctgcccgc cacgcccggt
     2701 gagtccgagg ctgctagaac ctgagtggcg atgagcgcgg acaccatccg aagcgcgtac
     2761 gtggcgctcg gtgagggcga tgtcgagccc ctcgtcgcgt tgatggaccc tgagatgggc
     2821 tggcacggtt atcgggggtg gcgcttctgg cgtccgcggc cctcctgaca cgggccgagc
     2881 gaggcccgtg aggtcctcac gtcagccatc gagcacggac gacggagcgg cggacacgac
     2941 ccgcgcgtcg agctcgtcga gcagcatggc gagcaaactg ccgtcgtgat gtcgtgggcc
     3001 acatcgactg accgtcacga gatggcacaa gtcctgcaga tctccaacgg gaagatcgtc
     3061 cggatgcggg actacccgag ccggaagaag gcgctgcgtc ggctgcgccg agccgcttcg
     3121 ccgtagggtc caccgcatcg gcgttcctgc tcccagtccg gcatacactc aagccgcggc
     3181 ggggtgccgg agcggtcgaa cggggcggtc ttgaaaaccg ttgagccgag aggctccgtg
     3241 agttcgaatc tcacccccgc cg
//
LOCUS       DBNGNK010000285        12447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_692963,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000285 DBNGNK010000000
VERSION     DBNGNK010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12447
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_692963"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..174)
                     /locus_tag="AC150V_08325"
     CDS             complement(<1..174)
                     /locus_tag="AC150V_08325"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR3762965.1"
                     /translation="MKTRAGLFWEVGRPLEVAEIDLEEPGPDDVVVRMAAVGICGTDL
                     HQVKGDFQRPTPMV"
     gene            complement(179..1727)
                     /locus_tag="AC150V_08330"
                     /pseudo
     CDS             complement(179..1727)
                     /locus_tag="AC150V_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            2072..2401
                     /locus_tag="AC150V_08335"
     CDS             2072..2401
                     /locus_tag="AC150V_08335"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR3762966.1"
                     /translation="MEADYQHGLELIERGEYFAAHEVLEDVWREAAAEERDFYQGLVH
                     VAVAWYQAGRDNRRGCERQLEKAARRLSPYAPVHRGLDVADLVAQVRAAAAAYPDLPR
                     PRLHRAS"
     gene            2513..3232
                     /locus_tag="AC150V_08340"
     CDS             2513..3232
                     /locus_tag="AC150V_08340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR3762967.1"
                     /translation="MGDTVIFIPAWNEEQNLPSVLDELNSELPGRDVIVIDDGSTDGT
                     AEVAREHGADVVSFGYNRGLRVGIAEGYRQAHERGYAYCGRVDADGQHPAAELRRLLE
                     LVRSGDCDVAIGSRFASGDGYDVGRYEPSPARKLGIGVLQKSMHAVLRRPFRDPTSGL
                     TAANAMALPIMAEPYTSGAPEVEALIRLHDAGLAVEEVPVHMRERAGGESKLRGSKAV
                     KLVATVAATIIGYGLYRRLRR"
     gene            3229..3729
                     /locus_tag="AC150V_08345"
     CDS             3229..3729
                     /locus_tag="AC150V_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR3762968.1"
                     /translation="MSTSTGRVIVVLGYSKRGAASLDPVCRARLERAAEVSRPDDVVV
                     LSGWSRGGRGRPEAELMAEAWNAPVAEVVVDPDARSTVGNASNALNDVLRVHAREVVI
                     VTSAWHAPRARAAFRWLLRGTGVRVASASPAGRSWQASLRELLLWPLLPFQLEAARRS
                     SSRANS"
     gene            complement(3994..4743)
                     /locus_tag="AC150V_08350"
     CDS             complement(3994..4743)
                     /locus_tag="AC150V_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3762969.1"
                     /translation="MSLLKRLAALKEAEERGVLFTVVEGDGIGSNLLVLESGERLGEG
                     PEEAAAQFDELVRGGRNRLLELSDGSKVFAEWYGPPPRLLVYGAVDTAEALCRAANLL
                     GWTTIVADARAKFLTRERIPSADVLIGKWPEETFSEVAPDHQTAIVVLTHDDKFDEPA
                     LIGALETEAFYVGALGSRRNQERRRERLLEAGVAAESLDRIMGPCGLDIGADTQEETA
                     LSILAEILAVRARRTGGFLKVAKQRIHVEVE"
     gene            complement(4740..5051)
                     /locus_tag="AC150V_08355"
     CDS             complement(4740..5051)
                     /locus_tag="AC150V_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR3762970.1"
                     /translation="MKEILPEVERWKDEGEKVVVATVVATRRSAPRPVGTSLAVSESG
                     KMCGSVSGGCVESDVFESALEVMESGEPKLLSYGISDDLAWSVGLPCGGEIDVFVERI
                     E"
     gene            complement(5048..6184)
                     /locus_tag="AC150V_08360"
     CDS             complement(5048..6184)
                     /locus_tag="AC150V_08360"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3762971.1"
                     /translation="MADALVRHVVTFGRVLREVGIEVGPGRVIDALRGLDRVELTSQP
                     DVYWTLRQTLVSRHDELDLFDRAFAAWFLREPIYPEGRGAVPEPVSMRRIVQSTADAA
                     RADDEEDEGPDEPLELGSSHHELLREKDFAEMTPEEFERARRLIAAIARTRPKRASRR
                     RCADPRGDVLDMRRLVRRSLRAGGDPLERPYRARKEVPRKLVVLCDVSGSMESYTRAL
                     LLFLHAVVGTGRGVEAFAFGTRLSRLTPELATRDPDAALEKATEAVVDWGSGTRIGAS
                     LKEFNDVYGRRALSRGAVVVIVSDGWERQDPASVGREMARLSRAAYAVIWVNPLRGNP
                     EYQPLAGGMRAALPFVDRFLPGHNLQSLEELATVLAGIERRHAA"
     gene            complement(6190..7071)
                     /locus_tag="AC150V_08365"
     CDS             complement(6190..7071)
                     /locus_tag="AC150V_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014208547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3762972.1"
                     /translation="MRFETIDELEGALRDADYLPDRGLSTALFLALELEKPLLLEGEA
                     GVGKTEAAKALARAVDATLIRLQCYEGLDVQHAVYEWNYPRQLLHIRAAQEGTVSEDE
                     LFGAEFLIRRPLLEAIDSDEPVVLLIDEIDRADEEFEAFLLEVLSDFQITIPELGTIS
                     ARRRPAVILTSNRTRELHDALKRRALFHWIGHPSIEREIEIVKLRVPDIPERLASEAA
                     AFVRGLRGLDLAKPPGVAETIDWAHALAALGRREIDAEVVEQTLGSVLKYREDIEAVR
                     DDTLAALVDEARTAGTR"
     gene            7199..8104
                     /locus_tag="AC150V_08370"
     CDS             7199..8104
                     /locus_tag="AC150V_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3762973.1"
                     /translation="MAYEIKLLDLIDIELEASFLVLGRGMGTTARVKTWGYLIMGPDT
                     DPILVDTGASHPEIMERLGMKGIVTKEMTLRRQLAKHGVKINEVRWILHTHHHIDHAG
                     QDSQFSYKQTVITNRRELEYSASGIMGGQYPPEYVKHHIDRLHRPGAMRLLDLELSGP
                     DEIAPGIVCEAAGGHTEGSMNILVETKEGTACICGDVIYDVQNQIVDPIYQVLDHEPQ
                     PTGNHGTNKRQEKAAVKKALNSGVFCLPIHDWPARVESGRVVSRFVGDSVPGREVRVE
                     HRTTSETQKPGAGAEMWTPPLAAAP"
     gene            8140..9087
                     /locus_tag="AC150V_08375"
     CDS             8140..9087
                     /locus_tag="AC150V_08375"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3762974.1"
                     /translation="MEAVRAALVDALRDDDRVFLLGEDIGVFGGAFGVTAGLVDEFGS
                     DRVLDTPIAEEGFVGAALGAAWMGERPVVELQFADFATCPFDMIVTVGAKTHWRSGAA
                     LPVVIRAPSGGGVRGGPFHAGSPEGLFVGQPGLKVVCPGTVADAYGLLRAAIDDPNPV
                     LYFEHKALYRRLRAEKPEPGYRVPLGTAKIARAGTDATIVTYGAGVGLALEAAERVEA
                     ELEILDLRTVWPFDSDVVLGSLEKTSRVLLLQEASQSTGVAGLVLALIAREGFELLDA
                     PPALLAPPDTPVPFAPELEDAYLPSIDSTVGALEKLLAY"
     gene            9077..10102
                     /locus_tag="AC150V_08380"
     CDS             9077..10102
                     /locus_tag="AC150V_08380"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3762975.1"
                     /translation="MPTEPCVDLPAVRGEERLALFRLVLLQRLFEERALTLYRQGRIA
                     GSFYDGRGQEAVAAGAGLALGPDDVVCPLNRELACHFARGVTPADAFRNFLGRADGPT
                     FGRDGNMHFGAPEKGVFPLVSMLGDLVPVTVGAALAFKRRGEARVAMTFVGEGAFSVG
                     DTSEGLNLAGVWRVPAVFVVQSNRYSYSTPVERQMVNTNIAQRIYGGWSIPAERIDGS
                     DAIAVFDAVRAAVERARNGNGPQAVEALSLRVHGHAAHDDGSYVPAGVREAYEEHFDP
                     VERLAARLLADGLDHGEVDDLRAAARGEVEHGLSEAEASPAPDPATLEQGVYAERSSG
                     QGSSSQP"
     gene            10217..10405
                     /locus_tag="AC150V_08385"
     CDS             10217..10405
                     /locus_tag="AC150V_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762976.1"
                     /translation="MASNAAVPWRPLAWEPFDVALPETALAALDPLLAELERRRGQIE
                     ALGGYDLTPSGEITRVGR"
     gene            10504..11292
                     /locus_tag="AC150V_08390"
     CDS             10504..11292
                     /locus_tag="AC150V_08390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3762977.1"
                     /translation="MTQASEVLFPTSPGEAAAAFGDGTDVTVIGGGTIVLPEITYGRL
                     RPTKAVVLSRAGLAGVSRKGSRVTIGAATPVQDLVDLGAPVGPCAANVADVEIRSQGT
                     LGGNLCAGEGPEAPRGDLQGALLAVAAEITSAGAGGEQTESLEDFMPHRYERLILSVS
                     YEEPAAGAFVALDRPHTHDYTAVGVSAARLADGTVRVAATGVGWFGVRLPSAEAKADE
                     PGAAGEAALQDVSLHDDALASAWYRQKTLPILVARALTTLKEAA"
     gene            11289..11804
                     /locus_tag="AC150V_08395"
     CDS             11289..11804
                     /locus_tag="AC150V_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011457280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3762978.1"
                     /translation="MKLTVNGIEREIESSLLKPLLFVLREELGVTSPKAGCQQGGCGA
                     CTVLIDGEPRRSCLTAVGTIEGASITTVEGVGTPDHLAPIQQAFTHHYAAQCGFCTSG
                     YIMAAQAYLDGGGKDDTDAIKEALAGHVCRCTGYVKILDAVAAAARGDEFDLDVTAAG
                     QYTTMKSGGAE"
     gene            11801..>12447
                     /locus_tag="AC150V_08400"
     CDS             11801..>12447
                     /locus_tag="AC150V_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013482.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR3762979.1"
                     /translation="MKAVGARLPRYDGVGHVTGRTVFVDDVRVPGTLWVKALRSPVHR
                     ADIKRLDTSKAESAPGVGAVITWEDVPLLEYGHLSALGIPADEPLLAKDDVRYLGQPI
                     ALVAAESEEAAQAAVESIELDLDEQEPLFDVRKAFAKDAPKVHQWGNWYPHFEAEMDR
                     RQIRKGDIDRAFDQADSIIKGVYRPAAIEHVPLETQVCQVVPEPDGRLTIYSCTQA"
BASE COUNT         1935 a         4309 c         4237 g         1966 t
ORIGIN      
        1 caccatgggc gtcggccgct gaaagtcgcc cttgacctgg tgcagatcgg tcccgcagat
       61 gccgaccgcc gccatgcgga cgacgacgtc gtccgggccg ggttcctcga ggtcgatctc
      121 ggcgacctcg agtggccgcc cgacctccca gaagagtccg gccctcgtct tcaccgtcct
      181 acgctcccag cgctgcctgc accgatgctc cgttcgtcac ggtggagacg ttctgaagcg
      241 cgaagttgat cgacgcgttg tgccagtccg cattcatcgt cgagcagcag tcctcgggta
      301 cgaccatgaa gtagcccttg tccgcaccgg tgcgggctgt gtgctcgatc gacatgttcg
      361 tccacgcacc ggtgacgatg atcgtgtcgc ggccgagccc ggtcagcagc gtctcgagct
      421 tcgtgccctg ccaaccgctc atgcgcatct tctcgaccac gtgatctccg tcctgcggct
      481 cgagcccgtc cgccggagcg gccccccagg tgccgcgcac gaggccgttc gtgtccttga
      541 cgccctggaa gagcggcgcg ttcaacttca ggcccggcgc gccctcctcg acgatgtacc
      601 agacgtggat caccgcgacc cccttcgagc ggcaggcggc ggcgagtttc ttgacgttct
      661 cgacgacgtt ctgctccttt gcgtgttggg gcgacccgga ttccgcaaag gcgccgccgt
      721 cgatgatcac gtcgttctgc aggtcctgga tgaggagcgc gcagcggctc ggatcgagct
      781 tcagttcgtc gttcacggtc tctccttcct cgcccagccg gctagcccat gtagggctcg
      841 cggcgcgagc gcaccttggt gcgaatcgag tagagggacg tggagctcgg caggtacagc
      901 gtctgccagt cctcgccgcc ccaggtcagg ttgccgacgt tctccggaac ctcgatgacg
      961 ccgagatgct cgccggagga gctgatcagc caaacgccgc ccgggcccgt gacccagacg
     1021 ttgccccgct cgtcgcactt catcccgtcc ggaatgccct cctcgatcac gccggagccg
     1081 atgccctcga agaacatgcg gccgttcgag atcgagccgt ccgtgttcac gtcgtagacc
     1141 ttgatgagcg cattgggcgt gtcgttgatg tagagcagcg actcgtcggg cgagaagcag
     1201 aggccgttgg gcatctcgta ctcgtcgcga ccgacgacga gctcgagatc cccgcctccc
     1261 ggtgggatcc ggaagacgcc ctgccagccg aggtcgcgct cgcgctcctc accgaacacg
     1321 ggcatgcggc cgtaccacgg gtcggagaag tagatcgacc cgtccgaacg gacgacgacg
     1381 tcgttcgggc tgttcagctc cttgccctcg aagtgagagg cgatggtctc gcgcgcgccg
     1441 tcagggctct cgcggacgac tgtgctcgtc acgtgctcgc acacgagcag gttcagatct
     1501 gcctcgtacg tcagtccgtt gcacttgttc gaggggttca tcacctcgac gacggttccg
     1561 tccggtgtgt acttgcggcg gatgtccgcc ggcatgtccg agaagagcag gtagtgctcc
     1621 ttcggatgcc agatcgggcc ctcggtgaag gtgaagcctg tcgccaggcg ctcgactccc
     1681 gagccttcct cgacaaggtc gtagatcgcg ggcgatttcg cgtcgattcc cggcacgttg
     1741 atcccctttc ctggtgaaag acgtcgggtt cctggtgaaa gacgtcgggc cgactctaca
     1801 acggttgggg atggccggat gggcgccagg tgtcgcgggc tggagccccg tgatccgcgc
     1861 ttcgcaccag ctccccggag actcgtctct cgtcgcacgc accgaggtgg cgagactcag
     1921 cggatgggtg cacaatcact tcgcgcccgc gacgcatgcg ggcgcccgag gcgaagaggt
     1981 ctcccgcgct gtccaagacg caaatgctcg gtgagcggct gccggtgatt ccggactcgc
     2041 cgaccgcgat ccagtcgacg tcccgcatgt gatggaagcc gactaccaac acgggctgga
     2101 gctgatcgag cgcggcgagt acttcgccgc gcacgaggtc ctcgaggacg tctggcgcga
     2161 ggccgcggcc gaggagcgtg acttctatca gggactcgtc cacgtcgccg tcgcctggta
     2221 ccaggccggg cgggacaacc gacgtggttg tgagcgtcaa ctcgaaaagg cggcccgtcg
     2281 gctctctccc tatgcaccgg tccaccgcgg gctggacgtc gccgatctcg tcgcacaggt
     2341 tcgcgcggcg gccgccgcgt atcccgacct gccgagaccg cgactgcacc gcgcttccta
     2401 ggacgcacgt cggcgctcgg atgagccacg ctgagcacgg gcgctccgtc ggatgagagc
     2461 gttctgagca ccgtagacgg cttgtttccg ggcgattggt agggtcggcc cgatgggcga
     2521 cacggtcatc ttcattccgg cctggaacga ggagcagaat cttccgtcgg tgctcgacga
     2581 gctgaacagc gagctgccgg gaagggacgt gatcgtgatc gacgacgggt cgaccgacgg
     2641 gacagcggag gttgcccgcg agcacggcgc cgacgtcgtc tcgttcggct acaaccgcgg
     2701 tctccgtgtc gggatcgccg agggctaccg gcaggcgcac gagcgcggct acgcgtactg
     2761 cggacgggtc gatgccgacg gacaacaccc tgccgccgag ctgcgacggc tcctagagct
     2821 cgtgcgatcc ggcgactgcg acgtcgcaat cggttcgagg ttcgcctccg gggacggcta
     2881 cgacgtgggc cgttatgagc cctcgccggc gcggaagctc ggaatcggcg tgctgcagaa
     2941 gtccatgcat gccgtgctgc gccgaccgtt ccgcgacccg acgagcggac tgacggccgc
     3001 gaacgccatg gcgctcccga tcatggccga gccgtatacg agcggcgccc ccgaggtcga
     3061 ggccctgatc agacttcacg atgcagggct cgccgtcgag gaagtgccgg tccacatgcg
     3121 cgaacgggcg ggcggcgagt ccaagctccg cggatcgaag gcggtgaagc tcgtggccac
     3181 cgtcgctgcg acgatcatcg ggtacggcct gtaccgccga ctccgcaggt gagcaccagc
     3241 accggccggg tgatcgtcgt cctcggctac tcgaaacggg gggccgcgag cctcgacccc
     3301 gtctgccggg cgcggctcga gcgggcggcg gaggtctccc gccccgacga cgtcgttgtg
     3361 ctctccggct ggtcgcgggg cgggcgcggg cggccagagg ccgagctgat ggcggaggca
     3421 tggaatgcgc cggtcgcaga agtggtcgtc gaccccgacg cgcgctcgac cgttgggaac
     3481 gcctcgaacg ccttgaacga cgtcctccgc gtgcacgcgc gggaagtggt catcgtcacg
     3541 tccgcgtggc acgctccgag agcgagggcc gctttccgct ggctgctccg tggtaccggc
     3601 gtacgcgttg catccgcatc tccggcgggg aggtcgtggc aggcttcgct gcgcgagctg
     3661 ctcttgtggc cactgctgcc gttccagctc gaagcggcgc gccggtcgag cagccgcgcg
     3721 aactcgtgac ggcgcccgct gattgctggc cgcagagttc cgcgcgagct gatggcgccg
     3781 ggccggagcc cttcagcctt gttcgctccc gccgcgtctt tcgcacaggt cctgacatcc
     3841 agacacggcc cgtcgcgcgt ccgccgcgtt ccgcgatccc gctgcaggtc tatgaaccgg
     3901 actcttcggc cgaggccgaa tcggcctcgg cggacctcag aggtgggaca gtggccgccg
     3961 cggccagggg gtcgcgagcc gctgcggagt gggctactcg acctcgacgt ggatgcgctg
     4021 cttcgcgacc ttgaggaagc cgccggtacg gcgcgcccgc acggcgagga tctcggcgag
     4081 gatggagagt gccgtctcct cctgtgtgtc cgcgccgatg tcgagcccgc acgggcccat
     4141 gatccggtcg agcgactccg ctgcgacacc cgcctccaac agccgctcgc ggcggcgctc
     4201 ctgattgcgc ctcgacccga gcgccccgac gtagaaggcc tctgtctcca aggcgccgat
     4261 gagagccggc tcgtcgaact tgtcgtcgtg cgtcagcacc acgattgccg tctggtggtc
     4321 gggagccacc tcgctgaacg tctcctcggg ccacttgccg atcaggacgt ccgccgatgg
     4381 gatccgctcg cgcgtgagga acttcgcccg cgcgtcggcg acgatcgtcg tccagccgag
     4441 caggttggcc gctcggcaga gcgcttctgc cgtgtcgact gccccgtaga cgagcaggcg
     4501 cgggggcggc ccgtaccact ccgcgaacac cttcgatccg tcggagagct ccaggagacg
     4561 gttgcgcccc ccacgtacga gctcgtcgaa ctgcgcagct gcctcttccg ggccttcgcc
     4621 cagccgctcg cccgattcga ggacgaggag gttcgaccct atgccgtctc cctccaccac
     4681 ggtgaagagg acgccccgct cctccgcttc cttcagcgcg gccagccgct tgagaaggct
     4741 cactcgatgc gctccacgaa gacgtcgatc tcgccgccgc acgggaggcc gacgctccag
     4801 gcgaggtcgt ccgagatgcc gtaggagagc agcttcggct cgccggactc catcacctcg
     4861 agcgcgctct cgaacacgtc gctctcgacg cagccgcccg agacggagcc gcacatcttc
     4921 cccgactccg acacggcaag gctcgttccg acgggacgtg gcgcggaccg tcgcgtcgcg
     4981 acgaccgtgg cgacgaccac cttctcgccc tcgtccttcc agcgctcgac ctcgggcagg
     5041 atctccttca cgctgcgtgc ctcctttcga tgccggcgag cactgtcgcc agctcctcaa
     5101 gactctgcag attatggccg ggaagaaaac ggtcaacgaa gggcagcgcg gctcgcatcc
     5161 cgcccgcaag cggctggtac tcgggattgc cgcgcagcgg attcacccag atgacggcat
     5221 aggccgcccg cgagagcctg gccatctcgc ggccgaccga agccgggtcc tggcgctccc
     5281 acccgtcgga gacgatcacg acgacggcgc cgcgcgacag agcccgccgc ccatacacgt
     5341 cgttgaactc tttcagcgag gctccgatcc gcgtgccgct cccccagtcc accacggcct
     5401 ccgtcgcctt ctcgagcgcc gcgtcagggt cgcgcgtcgc gagctcgggc gtcaaccgag
     5461 agagcctggt gccgaacgcg aaagcctcca cgccccttcc cgtgccgacg accgcgtgca
     5521 gaaagagaag gagtgcccgt gtgtacgact ccatcgaccc ggagacgtcg cagaggacga
     5581 cgagcttgcg cggcacctct ttccgcgctc gatagggacg ctccagcggg tcgccgcccg
     5641 cacgcagcga gcggcgcacg agccggcgca tgtcgaggac gtcaccgcga ggatcggcgc
     5701 agcgacgacg cgacgcgcgc ttcggcctcg tgcgtgcgat cgccgcgatc aagcgacgcg
     5761 cacgctcgaa ctcctcgggc gtcatctcgg cgaagtcctt ttctcgcaac agctcgtgat
     5821 gcgacgagcc gagctccaac ggctcatcgg gaccctcgtc ttcctcgtcg tcggcccggg
     5881 cggcgtcggc agtggactgc acgatccgtc gcatcgacac cggttccggg acggcgcctc
     5941 gcccttcggg atagatcggc tcgcgcagaa accacgctgc gaacgctcga tcgaacaggt
     6001 cgagctcgtc gtggcgcgag acgagcgtct gccgaagcgt ccagtagacg tcgggctggc
     6061 tcgtgagctc gacgcggtcg aggccgcgca gagcgtcgat cacccgcccg ggaccgacct
     6121 cgatgccgac ctcgcgcagc acacgaccga acgtgacgac atggcgaacg agcgcgtcgg
     6181 ccatgaccgc taccgcgtgc cggcggtccg cgcctcgtcc acgagagcgg ccagcgtgtc
     6241 gtcccgcacc gcctcgatgt cctctcggta cttgagcacg gagccgaggg tctgctcgac
     6301 cacttccgcg tcgatctcgc ggcggccgag cgcagccagc gcgtgcgccc agtcgatcgt
     6361 ctcggcaaca cccggcggct tggccaggtc gaggccgcgc agaccgcgaa cgaaggcggc
     6421 ggcctccgag gcgagccgct ccggaatgtc cggtacccgc agcttcacga tctcgatctc
     6481 gcgctcgatc gacgggtggc cgatccagtg gaagagcgcg cgccgcttga gcgcgtcgtg
     6541 cagctctctc gtccggttcg aggtgaggat caccgccggc cgccgccgcg cgctgatcgt
     6601 gccgagctcc ggaatcgtga tctggaagtc ggagagcacc tcgagcagga atgcctcgaa
     6661 ctcctcgtcg gctcgatcga tctcgtcgat cagcagcacg accggctcgt cggagtcgat
     6721 cgcctcgagc agcggtcggc ggatcaggaa ctcggccccg aagagctcgt cttccgacac
     6781 cgtcccctcc tgcgccgccc ggatgtggag gagctggcgc ggatagttcc attcgtacac
     6841 cgcgtgctgg acgtcgaggc cctcgtaaca ctgcagccga atcagcgttg cgtcgactgc
     6901 gcgggcaagc gctttcgccg cctcggtctt gccgactccg gcctcgccct cgagcaggag
     6961 cggcttctcg agctcgagcg cgaggaagag cgcggtggac aggccccggt cggggaggta
     7021 gtccgcgtcg cggagcgcgc cctccagctc gtcgatcgtc tcgaatctca tggacggagc
     7081 ctagccccta cgtgtcagac ggcgcattcg cggagcgcgc gacgcccggt cgtctcgaga
     7141 cgtttgactg cggccctcat ctctgaacca atggagcctc accgaaagga gggccgtgat
     7201 ggcctatgag atcaagcttc tcgacctgat cgacatcgag ctcgaggcga gcttcctcgt
     7261 cctcgggcgc ggaatgggaa cgaccgcccg tgtgaagacc tgggggtacc tgatcatggg
     7321 gcccgacacc gacccgatac tcgtcgacac cggcgccagc catcccgaga tcatggagcg
     7381 gctcggcatg aaggggatcg tgacgaagga gatgaccctc cgtcgccagc tcgccaagca
     7441 cggcgtgaag atcaacgagg ttcgctggat cctccacacg catcaccaca tcgaccatgc
     7501 cggccaggac tcccagttct cgtacaagca gacggtgatc accaaccggc gcgagctcga
     7561 gtactcggcc tcgggaatca tgggcggcca gtatccaccc gagtacgtca agcaccacat
     7621 cgaccgcctg caccgtcccg gcgcaatgcg attgctggat ctcgagctgt ccggtcccga
     7681 cgagatcgcg cccggcatcg tctgcgaggc ggccggcggt cacacggagg gctccatgaa
     7741 catcctcgtc gagacgaaag agggaaccgc ctgtatctgc ggcgacgtca tctacgacgt
     7801 ccagaaccag atcgtcgacc ccatctacca ggtgctcgac cacgagccgc agccgacggg
     7861 caaccatggg acgaacaagc ggcaggagaa ggctgccgtc aagaaggcgc tcaactcggg
     7921 agtgttctgc ctgccgatcc acgactggcc cgccagggtg gagagcggcc gcgtcgtttc
     7981 ccgcttcgtg ggcgactccg tgccggggcg cgaggttcgc gtcgaacatc ggactacgag
     8041 cgagacgcag aagcccggcg ccggagcgga gatgtggacg ccaccgctgg ccgccgcgcc
     8101 gtgaccatct aggctctcac gatcgccgag atcacctact tggaggccgt tcgggcggcc
     8161 ctcgtcgacg cgctgcgcga cgacgaccgg gtgttcctgc tgggtgagga catcggggtg
     8221 ttcggcgggg cgttcggggt caccgccggt ctggtggacg agttcggcag cgatcgggtg
     8281 ctcgacacgc ccatcgccga ggaggggttc gtcggcgccg cgctgggagc tgcgtggatg
     8341 ggcgagcggc ccgtcgtcga gctccagttc gccgatttcg ccacgtgtcc cttcgacatg
     8401 atcgtgacgg tgggcgcaaa gacccactgg cgctcgggag cggcgctgcc ggtcgtgatc
     8461 cgcgccccct cgggcggcgg cgtgcgcggc ggcccgttcc acgccggctc gccggagggg
     8521 ctattcgtcg ggcagcccgg cctgaaggtc gtctgcccgg gcaccgtcgc cgatgcgtac
     8581 ggactcctgc gcgccgcgat cgacgatccg aatcccgttc tctacttcga gcacaaggcg
     8641 ctctaccgcc ggctgcgagc cgagaagcca gagcccggtt accgcgtccc gttgggcacg
     8701 gcgaagatcg cgagggcggg gaccgacgcg acgatcgtga cctacggcgc cggcgtcggc
     8761 ctcgcgctcg aggcggcgga acgcgtcgaa gccgagctcg agatcctgga tctgcgcacg
     8821 gtatggccat tcgactccga cgtcgtgctc ggatcgctcg agaagacctc gcgggtgctc
     8881 ctgctccagg aggcctcgca gtcgaccgga gtggcagggc tcgttctcgc gctgatcgcc
     8941 cgcgagggct tcgagttgct cgacgctccg ccggcactcc tcgcgccgcc cgatacgccg
     9001 gtgcccttcg cgccggagct cgaggacgcc tacctcccct cgatcgactc gaccgtcggt
     9061 gccctggaga agctccttgc ctactgagcc gtgcgtcgac ctcccagcgg tacgtggcga
     9121 agagcggctg gcgctcttcc gcctcgtgct gctccagcgg ctgttcgagg agcgggcact
     9181 gacgctctac cggcagggcc gcatcgcggg ctcgttctat gacggccgcg gccaggaagc
     9241 ggtcgccgcg ggagcggggc tcgcgctcgg gccggacgac gtcgtctgcc ctctcaaccg
     9301 tgagcttgcg tgccacttcg cgcggggggt gacgccggcc gacgcctttc gcaacttcct
     9361 cggtcgagcg gacgggccca ccttcggcag ggacggaaac atgcatttcg gagctcccga
     9421 gaagggggtg tttccgctcg tgtccatgct cggcgacctg gttccggtca cggtcggcgc
     9481 cgcgctcgcg ttcaagcgcc gtggcgaggc ccgtgtcgcc atgacattcg tcggcgaggg
     9541 cgcattctcg gtcggcgaca ccagtgaggg cctgaacctc gccggtgtct ggcgggtgcc
     9601 ggcggtgttc gtcgtgcagt cgaaccgcta ctcgtactcg acgccggtcg agcgccagat
     9661 ggtgaacacg aacatcgcgc agcggatcta cggcggctgg tcgattccgg ccgagcgcat
     9721 cgacggctcc gacgcgatcg ccgtgttcga tgccgtccga gccgccgtcg agcgggctcg
     9781 caacggaaat ggcccgcagg cggtcgaggc gctctcgctt cgcgtccacg gccacgctgc
     9841 gcacgacgac ggaagctacg tgccggccgg ggtgcgggag gcctacgagg agcacttcga
     9901 ccccgtcgag cggctcgccg cccgtctcct cgcggacggg ctcgatcacg gcgaggtcga
     9961 cgacctccgt gctgcggcgc ggggagaggt cgagcacgga ctttcggagg cggaggcgtc
    10021 tcccgccccg gatccggcga cgctggagca gggggtctac gcggagcgca gctccggcca
    10081 ggggtcgagt agccagccgt agcccagctt gccggcctgc gaacgcaggt cgctgacact
    10141 gccgtcgagg acgatgcgcc cgccgtcgag aacgaccaga cgccgcgcca acgcagtcgc
    10201 gtcgcggacg tcgtgcgtgg ccagcaacgc cgccgtgcca tggcgcccgc tggcctggga
    10261 gccgttcgac gtcgcgctcc cggagacggc tctcgccgcg ctcgatccgc tcctcgccga
    10321 gctggaacgc cgtcgcggcc agatcgaagc attgggcggc tacgacctca cgccgtccgg
    10381 agaaatcacc cgcgtaggcc gctgacgcgc tctgctcccg ccacgatcga ggatcgatcg
    10441 ccgcgcccgt ggccccggta ctagactcgc gggacagttc gaacttcgac cgaaggattg
    10501 tccatgacgc aagcttctga ggtcctgttc ccgacgtcgc ccggggaggc ggcggccgct
    10561 ttcggcgacg ggacggacgt gaccgtgatc gggggcggga cgatcgtgct gcctgagatc
    10621 acctatggac gactacggcc gacgaaggcc gtcgtgctca gccgcgccgg cctcgccggc
    10681 gtcagccgca aggggtcgcg ggtgacgatc ggagccgcaa cgcccgtgca ggatctcgtc
    10741 gatctcggcg ctcctgtcgg gccgtgcgcg gccaacgtcg ccgacgtcga gatccgttcc
    10801 cagggcacgc tcggcggcaa cctctgcgcg ggcgagggcc ccgaggctcc gcgaggcgac
    10861 ctgcagggcg cgctgctcgc ggtcgcagcc gagatcacct cggcaggcgc cggcggtgag
    10921 cagaccgagt cgctcgaaga cttcatgccg catcggtacg agcgcctcat cctctcggtc
    10981 tcgtatgagg agcctgcggc cggcgctttc gtggcgctcg atcgaccaca cacgcacgac
    11041 tacacggcgg tcggcgtctc tgcagcgcgc ctcgcggacg gaacagtccg ggtcgcggcg
    11101 accggtgtgg gctggttcgg tgtccggctg ccgtcggccg aggcgaaagc cgacgagccg
    11161 ggcgccgccg gagaggcagc cctgcaggat gtgtcactgc acgacgatgc tcttgcgtcg
    11221 gcgtggtata ggcagaagac cttgccgatt ctcgtcgctc gtgccctgac gacgctgaag
    11281 gaggccgcat gaagctcacg gtcaacggca tcgagcgcga gatcgagagc tcgctgctga
    11341 aaccgctcct gttcgtgcta cgcgaggagc tcggcgtgac gagcccgaag gcaggctgcc
    11401 agcagggagg ctgcggcgcc tgcacggtgc tcatcgacgg cgagccgcgc cgttcctgcc
    11461 tcacagcggt gggcacgatc gagggagctt cgatcacgac cgtcgaagga gtcgggacgc
    11521 cggatcatct ggcaccgatc cagcaggcgt tcacgcacca ctacgcggcg cagtgcggct
    11581 tctgcacgtc ggggtacatc atggccgcac aggcgtacct cgacggcggc ggcaaggatg
    11641 acacggacgc gatcaaggaa gcgctcgccg gacacgtctg ccgctgcacg ggctacgtga
    11701 agatcctcga cgcggtcgcg gcagcggcgc gcggggacga gttcgacctc gacgtcaccg
    11761 ccgcggggca gtacacgacg atgaagtctg gaggtgcaga gtgaaagcgg taggcgcccg
    11821 cctgccccgc tacgacgggg tgggccacgt tacgggccga accgtcttcg tcgacgacgt
    11881 gcgggtacct ggaacgctct gggtcaaggc attgcgatcg cccgtccatc gagccgacat
    11941 caagcgactc gacacgagca aggcggagtc cgcccccgga gtcggcgcgg tcatcacatg
    12001 ggaagacgtg ccgttgctcg agtacggcca tctctccgcg ctcggtatcc cggccgacga
    12061 gccgctcctg gccaaggacg acgttcgcta cctcggccag ccgatcgcgc tcgtcgcggc
    12121 ggagagcgaa gaggccgccc aggcggccgt cgagtcgatc gagctcgacc tcgacgagca
    12181 ggaacccctg ttcgacgtac gcaaggcgtt cgccaaggac gccccgaagg tgcaccagtg
    12241 gggcaactgg tatccccact tcgaggcaga gatggatcgg cgacagatcc gcaagggcga
    12301 catcgaccgg gccttcgatc aggccgactc gatcatcaag ggcgtctacc ggcccgcggc
    12361 gatcgagcat gtgccgctgg agacgcaggt ctgccaggtc gttcccgagc cggacggccg
    12421 cctgacgatc tattcgtgca cgcaggc
//
LOCUS       DBNGNK010000286         7457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1553716,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000286 DBNGNK010000000
VERSION     DBNGNK010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1553716"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..853)
                     /locus_tag="AC150V_08405"
     CDS             complement(20..853)
                     /locus_tag="AC150V_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3762980.1"
                     /translation="MPAFLLALVGAAAWGTGDFFGGISSRRAHVLTVLAVSQLFGLLG
                     IVVWLAVTREAWPGIGGAAPAAAAGVAGAIGLTALYRGLALGAMAVVAPISAAAPVVP
                     LAVDAINGITPGAVQWLGIVLVLGGIGVLAREPGDEPNRLAAGVGLALVAALGFGTFF
                     VLIDSAADESVPWAIGTARLTATLLAAAVALVVGISLTPAVGVFPAIAAAGLFDACGN
                     VAIAAATTRGATGIVAVGSSLYPIVTIVLARLVLGEAVDAARRAGALVALAGAALVAT
                     G"
     gene            complement(859..1698)
                     /locus_tag="AC150V_08410"
     CDS             complement(859..1698)
                     /locus_tag="AC150V_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3762981.1"
                     /translation="MTTIAELAEDRVVEGVFAVAAKRRLRTRAGAPYLAFELVDPSGR
                     IDARIWNDVELLDRRFEEGDAIRVLGRVERFGGKLQVQVRSLEAAPDEDPATLTPALR
                     RDAEELDGFLEFLGDEIAHPELNSLVASFLGDPSLREALRALPAAGADGHHGYAGGLL
                     EHTVGVATLCRETAQLHPRLRADLLLAAALLHDLGRARELGRGPVFKQTEEGRLLGHV
                     HLGLRMVEERAATLEAAVRAELLHAIATHHDRAGARTAEAAVLYHANQLDAQAATRPV
                     GDD"
     gene            1735..2832
                     /locus_tag="AC150V_08415"
     CDS             1735..2832
                     /locus_tag="AC150V_08415"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR3762982.1"
                     /translation="MADVRPFHAVRYSDPSPSVVAPPYDVVDPAGLAELRARHQYNVV
                     HLTLAESELDAGALYQEWLDRGVLLREERPAYWLLAQDFVGPDGAARQRQGIVVSLRL
                     EPYASGVVLPHERTHAGPKESRLRLLRAARAQLEPVFLLHEREAPFSVPESPPELEAH
                     GTRLWRLEDGGSVADFFAGRQLLMADGHHRYETALAFHEEDGTAASARMMAVLMSTSD
                     PGLEIFPTHRVFSGRPDIAPSGEKAADPRAALELLEAESYDRAAAVAYRSGSTTLVHG
                     SAGELDVELVDEFGVDGISYTSDWHDAVARVDESSADVAFLLRATRIEDVFERARRGT
                     VLPQKTTYFFPKLVSGLLFHPLEPDGSGDAP"
     gene            2829..3626
                     /locus_tag="AC150V_08420"
     CDS             2829..3626
                     /locus_tag="AC150V_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3762983.1"
                     /translation="MSPWLGLCRAAVADIETELDRLPTRLEREPVLRAGEGGDDTTAV
                     DAAAEDAVVARLEELGGSFVLVSEELGERVFGSGGPTRVVVDPIDGSVNAKRGIPFFS
                     LSLAVADGPTMDDVAFGFVHDFGSGEEWTAERGAGAFLDGERLGAVRPKDRIELLAFE
                     GTTAEYIAASAPAMVGVAERLRVMGSLALSLCHLAAGRLDGVCSLKPARSIDIAAGQL
                     LVRETGLAIDLFEAPPLAAAPLDLGQRSRVVAAASHALCSELAAALS"
     gene            complement(3752..4354)
                     /locus_tag="AC150V_08425"
     CDS             complement(3752..4354)
                     /locus_tag="AC150V_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762984.1"
                     /translation="MSIRRFLEWGGIAAGIVLVGFGAFTLFLGVDARNTVRDSIKQEN
                     ITFGSAEDPAVAQYARQWEGEQVQTGEQARAFAQIIRTHALEGSGGLTYAQMGRFLAA
                     DNPDDPAGTSDPEAALKDEEGSPVSNSTRNTWVTATSLSTALNVSFMAERLSLFSIMV
                     GIALVLAGLGFIILAVVSLGHVFQEEESRTEAPRASPAPA"
     gene            4510..5589
                     /locus_tag="AC150V_08430"
     CDS             4510..5589
                     /locus_tag="AC150V_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR3762985.1"
                     /translation="MDPDRDAILKALESVYDPELQRPVTELDMVRDVVVAGADVTVTI
                     ALTVAGCPLRSSFQDQVAKHVGAVPGVESVRLEFDVMSAEEKAALSTKLRGGRPEKSI
                     SLEPGTRVVAIASGKGGVGKSSLTASLAAALAARGEKVGVLDADVYGHSIPHMLGVQQ
                     RPIVVDKMIVPPVRGELKLMSIGFFLDENSPVMWRGPMLHRALEQFLSDVHWGELDTL
                     VVDMPPGTGDVSISLGQLLPKAEVLVVTTPQPAAQQVAVRAAKMAQQTGMRLLGVVEN
                     MTYLAGTGQEVFGAGGGDALAEEIGAPVLGRIPLDPALREAADAGTPVNEAAPGSDAA
                     VAIEELAAAVAASRLGAIRKPLTVI"
     gene            complement(5653..6510)
                     /locus_tag="AC150V_08435"
     CDS             complement(5653..6510)
                     /locus_tag="AC150V_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762986.1"
                     /translation="MRRTLAIALAALTALTLAGIAGAVLRSSGVHETSATFDAKVERY
                     ESRTCTGTDEKTYQIVKARYAGNAASGEEGLNGPIVLHVRSVYNATDGVGYVHGKLKI
                     RRGEDQRRGWGRFWAVNKSGQLDGFVVGGVNHRYARLLGNLTANYNAASDGGFSEGKI
                     GGGAAGNGAALLVGHRCNGAKPPKNSVKLLVKGTVEEKSAESIKIKPFDGGESKTCKI
                     TGDSPKTDGIDPGDHVKAECKTSDLVLTRIHEYRSKRDAVADRKADAEKKDGDEKQQN
                     DDEEKRGDD"
     gene            6639..7310
                     /locus_tag="AC150V_08440"
     CDS             6639..7310
                     /locus_tag="AC150V_08440"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3762987.1"
                     /translation="MRAAFFDLDRTVIARSSALALAGAFRQSGLLSRNQLLRAALLQI
                     RFSRFGASEDKGREMAERGMAVLDGVEVTRVREIVAAALEPALKPLVYRGALQLVGRH
                     RERGERTYLVSAALDEVVAQLAAELGFDGSLGSTCGSTDGVFSGRPEFLCYGTSKAEA
                     LRSLAVGESLDLSVSTAYSDSHTDLPFLEAVGRPVAVNPDRELRRVATARAWPIVRFR
                     ARAFA"
BASE COUNT         1039 a         2571 c         2668 g         1179 t
ORIGIN      
        1 gcccccgggt gcggtacggc tagccagtgg cgacgagcgc agcgccggcc agcgcgacga
       61 gggcgccggc ccgccgcgcg gcgtccaccg cctcccctag tacgagccga gcgaggacga
      121 tcgtcacgat cggatagagc gagctcccga cggcgacgat gccggtggcc ccgcgcgtcg
      181 ttgccgcggc gatggccacg ttgccgcatg cgtcgaagag gccggccgcc gcgatcgcgg
      241 ggaagacccc tacggccggg gtgagcgaga tgccgacgac gagggccacc gccgcggcga
      301 gcagcgtcgc ggtcaggcgg gcggtgccga tcgcccacgg cacgctttcg tcggcggcgg
      361 agtcgatcag gacgaagaag gtgccgaagc ccaaggcggc gacgagggcg aggccgaccc
      421 cggcggccag cctgttgggc tcgtctccgg gctcgcgggc gaggactccg atccccccga
      481 gcacgaggac gatgccgagc cactggacgg ctccgggtgt gatcccgttg atcgcgtcga
      541 cggcgagcgg tacgacgggc gccgcagccg agatcggcgc cacgaccgcc atcgctccta
      601 gagcaagccc tcggtagagg gcggtcagac cgatcgcgcc ggcgacacct gctgccgcgg
      661 cgggtgcggc gccgccgatt cccggccagg cctctcgggt cacggccagc cacacgacga
      721 tcccgagcag gccgaagagt tgcgacacgg cgaggacggt caggacgtgg gcgcgccgcg
      781 aggagatccc gccgaagaag tcgcccgtgc cccaggcggc ggcgcccacg agcgcgagca
      841 ggaacgcggg caccgtgcct agtcgtcgcc gacggggcgc gtggccgctt gcgcatcgag
      901 ctggttcgcg tggtagagga ccgcggcctc ggccgtgcgg gcacccgccc tgtcgtggtg
      961 cgtcgcgatc gcgtgcagca gctcggcacg tacggcggcc tcgagtgtcg ccgcccgctc
     1021 ctcgaccatc ctcaggccga ggtggacgtg ccccagcagc cggccctcct cggtctgctt
     1081 gaacaccggg ccccggccga gctctctggc tctcccgagg tcgtgcagga gcgccgccgc
     1141 cagcagcaag tcggcgcgca accgggggtg cagctgagca gtctcacggc agagcgtggc
     1201 gaccccgacg gtgtgttcga gcagcccgcc ggcgtagccg tggtgaccgt cggctccggc
     1261 cgcagggagc gccctgagcg cctcgcgaag ggagggatcc cccaggaagg aggcaacgag
     1321 cgagttgagc tcgggatgcg cgatctcgtc tccgaggaac tcaagaaagc cgtcgagctc
     1381 ctcggcgtcg cgccggagcg ccggcgtcag cgtcgccgga tcctcgtcgg gcgcggcctc
     1441 cagcgagcgc acttgcacct gcagcttccc gccgaagcgc tcgacccggc cgagaacgcg
     1501 aatcgcgtcg ccctcttcga agcgtcggtc gagcagctcc acgtcgttcc agatccgggc
     1561 gtcgatgcgg ccgctcggat cgacgagctc gaaggcgagg tagggggctc cggcgcgggt
     1621 gcggagtctc cgcttggcgg cgaccgcgaa caccccttcg acgacccggt cctcggcgag
     1681 ctcggcgatg gtggtcacgg cgaggagtct acgagcagag gtaggctcgc gcggatggcc
     1741 gacgtccgtc cgttccacgc tgttcgctac tcggacccga gcccgtcggt ggtggcgcca
     1801 ccgtacgacg tcgtcgatcc cgcgggactg gcggagctcc gcgcacgaca ccagtacaac
     1861 gtcgtgcatc tgaccctcgc agagtccgag ctggacgccg gcgccttgta ccaggagtgg
     1921 ctcgaccgtg gcgtgctcct gcgcgaggag cgaccggcgt actggctcct cgcacaggat
     1981 ttcgtcggcc ccgacggtgc ggcgcgccag cggcagggga tcgtcgtctc gctccggctc
     2041 gagccctacg cgtccggcgt cgtcctgccg cacgagcgaa ctcacgccgg accgaaggag
     2101 agccggctcc gcctactgcg tgccgcgcgg gcccagctcg aacccgtctt cctgctgcac
     2161 gagcgcgagg cgccgttctc ggtgccggag agcccgcccg agctcgaggc gcacgggacg
     2221 cggctgtggc ggctcgaaga cggcggaagc gtggccgatt tcttcgctgg cagacagctg
     2281 ctgatggcgg acggacacca tcgctacgag acggcgctgg cgttccacga ggaggatgga
     2341 acggcggcca gtgcccggat gatggccgtg ctgatgagca cgtcggatcc ggggctggag
     2401 atctttccca cccaccgcgt gttcagcggc agacccgaca tcgcgccgag cggcgagaag
     2461 gcggcggatc cccgtgctgc cctcgagctg ctcgaggcgg agtcgtacga ccgcgctgcc
     2521 gccgtcgcct atcgcagcgg gagcacgact ctggtccacg gctcggcggg cgagctcgac
     2581 gtcgagctcg tcgacgaatt cggggtggac ggaatctcct atacgtcgga ctggcacgat
     2641 gcggtcgctc gcgtggacga gtcgtcggcg gacgtcgcgt tcctgctgcg ggcgacacgg
     2701 atcgaggacg tcttcgagcg ggcccggcgc ggcacggtgc tgccgcagaa gaccacgtat
     2761 ttcttcccga agctggtctc ggggctcctc ttccaccccc tcgagccgga cggcagcggg
     2821 gacgccccgt gagcccgtgg ctggggctct gccgcgccgc cgtcgccgac atcgagactg
     2881 agctcgaccg gctgcccacg cggctcgagc gggagccggt gcttcgcgca ggcgaggggg
     2941 gagacgacac gaccgcggtc gacgctgcgg ccgaggacgc ggtcgtcgca cggctggagg
     3001 aactcggcgg ctccttcgtg ctcgtctccg aggagctcgg cgagcgcgtc ttcggctccg
     3061 gtggcccgac ccgggtcgtc gtcgacccga tcgacggctc ggtcaacgcg aaacggggca
     3121 ttccgttctt ctcgctctcg ctcgcggtgg cggacggccc gacgatggac gacgtcgctt
     3181 tcgggttcgt ccacgacttc ggctcgggcg aggaatggac ggccgagcgc ggcgcgggcg
     3241 cgtttctcga cggagagcgg ctcggtgccg tgcggcccaa ggaccggatc gagctgctgg
     3301 cattcgaagg gacgacggcc gagtacatcg ctgcgagcgc cccggcaatg gtgggcgtgg
     3361 ccgagcgact gcgagtgatg ggctcgctgg cgctctcgct ctgtcacctc gcggccgggc
     3421 ggctcgacgg cgtctgctcc ctcaagccgg cccggtcgat cgacatcgcc gcgggtcagc
     3481 tgctcgttcg tgagacgggg ctcgcgatcg acctcttcga ggcgccgccc ctcgccgccg
     3541 cgccgctcga tctcggccag cgctcacgcg tggttgcggc ggcgagccat gctctgtgca
     3601 gcgagctcgc agccgccctg agttgacgag ccggcacgcg gcatgaggaa tgccgagagg
     3661 cggctttcgc cgcccctcgt cattgctccg ggaggtcgga gcaaacctcg cgagcctgcc
     3721 ttcactcgcg gttgcgttgc gcctcgccgc tctaggcggg cgccgggctc gcgcgcggcg
     3781 cttccgtcct ggactcctcc tcctggaata cgtgaccgag ggacacgacc gcaaggatga
     3841 tgaagccgag gcccgcgagg acgagcgcga taccgaccat gatcgagaac agcgacagcc
     3901 gctcggccat gaagctgacg ttgagcgcgg tcgacagtga cgtcgccgtc acccaggtgt
     3961 tgcgggtcga gttcgagacc gggctgccct cctcgtcctt gagcgcggcc tcggggtcgc
     4021 tggtgccggc cgggtcgtcc gggttgtcgg cggcgaggaa acggcccatc tgcgcgtacg
     4081 tgaggccacc ggatccttcg agggcgtgcg tgcgaatgat ctgcgcaaac gcgcgcgcct
     4141 gctcgcccgt ttggacctgc tcgccttccc actgacgcgc gtactgggcg acggcgggat
     4201 cctccgccga gccgaaggtg atgttttcct gcttgatgct gtcgcgcacc gtgttgcggg
     4261 cgtcgacacc gagaaacagc gtgaacgctc cgaacccgac gagcacgatg ccggcggcga
     4321 tgccgcccca ctcgagaaaa cgccgaatac tcatgcttgc tctacctccc ggatataggg
     4381 tgaactgtcg ttgcgtctag tatcgactct gcggcggcga gcttgtatcg tgctataccg
     4441 ctctagtcag cggaaaacgc acgcgcttgt tacggagaat tacggagcag ttgatacgct
     4501 ggaacgacgg tggatccgga ccgcgacgcc atcctgaagg cgctcgagag cgtctacgac
     4561 cccgagctgc agcgcccggt gacggagctc gacatggtcc gggacgtcgt cgtggcgggt
     4621 gccgacgtga cggtcacgat cgcgcttacg gtcgccggct gtccactgcg ctcgtcgttt
     4681 caggatcagg tcgcgaagca cgtcggggcc gtccccgggg tcgagtccgt gcggctcgag
     4741 ttcgacgtga tgagcgccga ggagaaggcg gcgctctcga ccaagctgcg cggcggccgg
     4801 cccgagaagt cgatctcgct cgaacccggc acccgcgtcg tcgcgatcgc gtccggtaaa
     4861 ggcggcgtcg gcaagtcgag cctcaccgcg agcctcgcag cggcgctcgc ggcccgaggc
     4921 gagaaggtcg gcgtgctcga tgccgacgtc tacggccact cgatcccgca catgctcggc
     4981 gtccagcagc gcccgatcgt cgtggacaag atgatcgtgc cgcccgtgcg cggcgagctg
     5041 aagctgatgt cgatcggctt cttcctcgac gagaacagtc ccgtcatgtg gcgcgggccg
     5101 atgttgcacc gcgcgctcga gcagttcctc tccgacgtcc actggggtga gctcgacacg
     5161 ctggtcgtcg acatgccgcc gggtaccggt gacgtttcga tctcgctcgg ccagctgctc
     5221 ccgaaggccg aggtgcttgt cgtgacgacg ccgcagccgg ccgctcagca ggtggccgtg
     5281 cgggccgcca agatggcgca gcagacgggg atgcgcctgc tcggtgtcgt cgagaacatg
     5341 acctacctcg cgggcacggg ccaggaggtc ttcggggccg gcggcggcga cgcgctggcg
     5401 gaggagatcg gcgcgcccgt actggggcgg atcccgctcg accccgcgct ccgggaggcg
     5461 gcggatgcgg ggacgcccgt caacgaggcc gcgccgggct ccgatgccgc cgtcgcgatc
     5521 gaagagctgg ccgcggccgt cgctgcgtcg cgtctcggcg ccatccggaa gccgctgacc
     5581 gtcatctgat cgcgcggatc agcgagaagg gcgcccgcag gcgcccttct ctcacgcggc
     5641 cttccggggg acctagtcgt cgccgcgctt ctcttcgtcg tcgttctgct gcttctcgtc
     5701 gccgtccttc ttctcagcat ccgccttgcg gtcggcgacg gcgtcccgct tgctgcggta
     5761 ctcgtggatc cgcgtcagca cgaggtcgct ggtcttgcac tcggccttga cgtggtcgcc
     5821 gggatcgatc ccgtcggtct tcggcgagtc gccggtgatc ttgcacgtct tgctctcgcc
     5881 gccgtcgaac ggcttgatct tgatcgactc ggcgctcttc tcctcgaccg tgcccttgac
     5941 gagcagcttg accgagttct ttggcggctt ggcgccgttg cagcgatggc cgacgagcag
     6001 cgcagcaccg ttgccggccg cgccaccgcc gatcttgcct tcggagaagc caccgtccga
     6061 cgcggcgttg tagttcgccg tcaggttgcc gagcagccgg gcgtagcggt ggttgacgcc
     6121 gccgacgacg aagccgtcga gctggccgct cttgttgacc gcccagaacc tgccccagcc
     6181 gcggcgctgg tcctcgccgc gccgaatctt cagcttgccg tggacgtagc cgacgccgtc
     6241 ggtcgcgttg tagacgctcc tgacgtgcag gacgatcggg ccgttcagac cttcctcgcc
     6301 gctggcggcg ttccccgcgt aacgggcctt cacgatctgg tacgtcttct cgtccgtgcc
     6361 ggtgcaggtc cgcgactcgt agcgctcgac cttggcgtcg aatgtcgccg aggtctcgtg
     6421 gacgccggat gagcgcagaa ccgcgcccgc aatgcctgcc agggtcagcg cggtgagcgc
     6481 ggcgagtgcg attgcgagtg tgcgacgcat gtgtgtcctc ctgatctcgt tccccgagtt
     6541 cgtcccccgc tcagcagtgt cggccgccgg tgtttgggcg aatggcggtc cccgtaagca
     6601 tttcctcagc attctgtgaa gccgctaacc tcgacgcctt gcgggccgcc ttcttcgatc
     6661 tcgaccgcac ggtgatcgcc cgctcgagcg cgctcgccct tgccggcgcg ttccggcaaa
     6721 gcggcttgtt gagccgaaat cagctgctcc gggccgcgct cctgcaaatc cgcttctcac
     6781 gcttcggagc gtccgaagac aagggccggg agatggccga gcgagggatg gcggtgctgg
     6841 acggcgtgga agtcactcga gtgagggaga tcgtcgcggc tgcgctggag ccggccctca
     6901 agccgctcgt ctatcgcggc gcgctccagc tggtcgggcg acaccgcgaa cggggagagc
     6961 gcacatacct ggtatcggcc gccctggacg aggtcgtcgc gcagctcgcc gcggagctcg
     7021 gcttcgacgg gtcgctgggc tcgacgtgcg gcagcacgga cggcgtcttc tccggccggc
     7081 ccgagttcct ctgctacggc acgtccaagg cagaggcgct ccggagtctc gccgtcggcg
     7141 agagcctcga tctctcggtc tcgacggcgt actccgacag ccataccgat ctgcccttcc
     7201 tcgaggcggt cggtcgtccg gtcgcggtca acccggaccg cgagctgcgg cgggtcgcga
     7261 cagcgcgcgc ctggccgatc gtgcgctttc gcgcgcgtgc gttcgcctga cgagtcggtc
     7321 taggagccga ccgagggcag gccggctagc gccatcgcga gctctgcctc gtcgtagtgg
     7381 cggtcctcga gcttgcccgc cgcatgatcc atgtacgcgc ccatgtcgaa gtggccgtgg
     7441 ccgcagaggt tgaagag
//
LOCUS       DBNGNK010000287         8233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_264776,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000287 DBNGNK010000000
VERSION     DBNGNK010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_264776"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC150V_08445"
     CDS             complement(<1..917)
                     /locus_tag="AC150V_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3762988.1"
                     /translation="MNLTSREQARVANEDRVEPQPEHGERRLADPGLTDLSWRDWLAV
                     VRRAAKQASEHQITDRAAALAYYAFLALPAALLVAVGVFTLTASPGSVTAVLERLDTV
                     APRETIVLVEDALVRVTEQNGTGMALIGFGFVLGLWTASGAMTAVMRSLNATYEMEES
                     RGFVRQRLVAAAMFFFILVALILVVGLLVLGPHLSDWIGDATGASGFVTWVWWGAQWP
                     ILIGGLLLAFAGVLAFGPNVEHPRFRFVTIGAVVAVVVWLAASGLFAFYVSQFGSYNK
                     TWGSLAAVVIMLTWLWLSGLVLLFGAEINA"
     gene            1124..1309
                     /locus_tag="AC150V_08450"
     CDS             1124..1309
                     /locus_tag="AC150V_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762989.1"
                     /translation="MAAREDLPPPVQRWNELVAAARMRCAVCGAPWHEAGERWRLVAT
                     GDEAEPQHVLLCPSCAA"
     gene            complement(1401..3404)
                     /locus_tag="AC150V_08455"
     CDS             complement(1401..3404)
                     /locus_tag="AC150V_08455"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR3762990.1"
                     /translation="MALVPPSVGPIARICAPAALALLALSGALATAGVPLRGAGSVLF
                     YGALLAATVTIALRPVAVREERAAWLAFGLGTGLWAGSELFIGLALDRAERELPSVAD
                     VGHLGTYVCYSLGIVLLLRSRMRRVETGFWLDGLIVALSAAAVGAAVLVQPVLEADDS
                     DTAATLTRLAYPVAGVVLLALAVGAFSIAARGSRRTWLLLCGGLAVLALADGFYVLQT
                     LGDVYQPGALLDFLWPAALVLVALAAWSDDRAVDGSEGRPVPAVPIGGAIVAIAVVTA
                     GSVVSISGHAVALAAGALAAVVVRLLVTFQQIDSLLARTRVEADTDVLTELPNRRKLL
                     ADLGDAIERATSEAPHVLALFDLDGFKLYNDSYGHPAGDLLLARLGEQLAQTVGDRGV
                     AYRLGGDEFCLLAEAGPAETAVILDEAVRGLRAKGAGFEINCSYGAVSLPGEASDAPA
                     AIRLADERLYAEKRRRRSERYRTHEPLLQALYERAPDLRQHVRNVVELALAVGRELKL
                     GADEITDLELAAELHDIGKLAVPDEILLKAGPLTEREWEFILQHPLVGERILAASPTL
                     SHVGAVIRATHERWDGEGYPGQLAGDEIPLPSRIISACDAVYAMVEQRPHQEPLPLPE
                     ALAELERCAGSQFDPDVIAALMLVVRRGSSELPITGGVESTAA"
     gene            complement(3478..3750)
                     /locus_tag="AC150V_08460"
     CDS             complement(3478..3750)
                     /locus_tag="AC150V_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3762991.1"
                     /translation="MSEGLRAMLGFLEKLTLRPGEVTPADAEAVRQAGVSEEAIEDAI
                     HVCVSFVLINKLADAFGWRMLDEDVYDQRAAIALERGYVIPPQALD"
     gene            complement(3838..4323)
                     /locus_tag="AC150V_08465"
     CDS             complement(3838..4323)
                     /locus_tag="AC150V_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR3762992.1"
                     /translation="MADRYTDKVDKLRNTTLAGPAETSAELRQAVAQRSAEAGRLGEP
                     ETPVPDDLRPYVDKVALHAYKVVDADVERLKGDGYSEDEIFEITVSASLGAALEHMTE
                     ALGIDAPPGVTATLLYRPEFFGKPYTDYLHACSRGDSEWSVGERELFAAFVSKLNQCP
                     W"
     gene            4374..4463
                     /locus_tag="AC150V_08470"
     tRNA            4374..4463
                     /locus_tag="AC150V_08470"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4408..4410,aa:Ser,seq:gga)
     gene            complement(4503..5129)
                     /locus_tag="AC150V_08475"
     CDS             complement(4503..5129)
                     /locus_tag="AC150V_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HIR3762993.1"
                     /translation="MLIYSLTTGAKVVLLIVAISFIVFALVVSMWIPRRKQDFPGEKK
                     LSAFLTITAALFIVQIGAVWWATGQEAEEHGDEAVATETAPAEPGTEAETTEAETTET
                     ATTETETTQTETTETETSETEPAIPVGDPAAGRDVYASAGCGACHALADAGSNGAIGP
                     NLDETQPSYDLVVDRVTNGKGAMPAFADQLSENEIADVAAYVATASGS"
     gene            5226..6440
                     /locus_tag="AC150V_08480"
     CDS             5226..6440
                     /locus_tag="AC150V_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014164.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR3762994.1"
                     /translation="MSDERLERYARLAVEVGVNLSPGQDLRISAHPEHLPLVRAIAKA
                     AYERGAHYVETTYGDPHVLQARIAHAPEDELGWSPPWQVALIDHLAEEHGAFISITGD
                     PEPELLADLDGSRVAKARPKKLAERVLSATGDGRIVWTIIACPTAGWAEAVFGSPDTE
                     RLWEAVAVTTRLDEPDPVASWREHVDRLTTRAALLDERRFDAIRFSGPGTDLTVGLMP
                     ESRWMTAAEESVRGQSFVVNMPTEEVFTSPDRRRAEGVVRATMPLAVGGRIVRDLEVR
                     FSGGRAVEVNGSNGADFVRQQMQTDEGAASLGEIALVDGDSRVGQTGIVFLDTLFDEN
                     AACHIAYGDGIRTAVEGGIDLDPEALTALGCNSSTVHTDFMIGGPEVAVDGIEAGGAA
                     VPILRDNDWVLS"
     gene            6506..6946
                     /locus_tag="AC150V_08485"
     CDS             6506..6946
                     /locus_tag="AC150V_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3762995.1"
                     /translation="MTSRLVSVSTEASAADAVARMVEAGVGSVVVLAGSRLEGIFTER
                     DVLSLAAAGADLGATGVVDVMTRAVVTASPEDDPVAVAHLMADNNVRHLPVTVEGHVL
                     GIVGIREILRVLLERAYESHDTDAHNTARDLLRRPSLSSPAEGA"
     gene            6937..7008
                     /locus_tag="AC150V_08490"
     tRNA            6937..7008
                     /locus_tag="AC150V_08490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6969..6971,aa:Val,seq:cac)
     gene            complement(7337..7585)
                     /locus_tag="AC150V_08495"
     CDS             complement(7337..7585)
                     /locus_tag="AC150V_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3762996.1"
                     /translation="MLDSNVGTLYGAGTNEDEIYVVRTDDSLLFEGVARVEVFRDVLS
                     AEGTVRLRLYAYRAFMPDRHPESICAISGTGLATPSSA"
     gene            complement(7755..>8233)
                     /locus_tag="AC150V_08500"
     CDS             complement(7755..>8233)
                     /locus_tag="AC150V_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR3762997.1"
                     /translation="GAGLIPPVYLGELYAEVARPDRPFADAIGSRPLPDAGTTITVTK
                     VATGGAVGVQATENTTLTEQDPDVNSLTANVATIGGFVDLSLQALERSMPGMDAVVFN
                     DLRAAYDSLLDRQLLSGSGSNGQHQGIRGVASIGSVTYTAATPTGAELLPRLYDGV"
BASE COUNT         1332 a         2834 c         2833 g         1234 t
ORIGIN      
        1 gcgttgatct cggccccgaa gaggagcacg agcccactga gccacagcca cgtgagcatg
       61 atcacgaccg ccgcgagcga gccccaggtc ttgttgtagg agccgaactg gctcacgtag
      121 aaggcaaaga ggccggaagc agccagccag acgaccacgg cgacgaccgc gccgatcgtc
      181 acgaagcgga aacgaggatg ctcgacgttc gggccgaacg cgagaacgcc cgcgaacgcg
      241 agcaggaggc cgccgatcaa gatcggccac tgggcgcccc accagaccca cgtgacgaaa
      301 ccggaagcgc cggtcgcatc cccgatccag tccgagagat gcgggccgag cacgaggaga
      361 ccgaccacca ggatgagcgc gacgaggatg aagaagaaca tcgcggccgc caccagacgc
      421 tggcgtacga aaccccggct ttcctccatc tcgtatgtcg cgttcagcga gcgcatgaca
      481 gcggtcatcg caccgctcgc ggtccagagg ccaagcacga agccgaagcc gatcagcgcc
      541 atcccggtgc cgttctgctc cgtgacccgc acgagtgcat cctcgaccaa gacgatcgtc
      601 tcccgcgggg cgacggtgtc aaggcgttcc agcacggcgg ttacagatcc ggggctggca
      661 gtcaacgtga agacgccgac tgccacgagc agggctgccg gcagcgcgag aaaggcgtag
      721 taggcgagcg ccgccgcccg atcggtgatc tggtgctcgg atgcctgctt ggccgcccgt
      781 cgcacgactg cgagccagtc ccgccaggag aggtcggtga ggcccggatc ggcgagtcgc
      841 cgctcaccgt gctccggctg gggctcgacg cgatcctcgt ttgcaacgcg agcctgctcg
      901 cgggaagtga ggttcatcgg ctcagccttc cttgaaaaac agcactggcg acggcgcgag
      961 gcacactcat atctcgtgcc tacccggacg gcggcgctcg taaacctccg ggaagactcg
     1021 cctcgcgatc gagcacgccg tttccactcg agggcgctgc tcagtgcccg cgcaacggcc
     1081 caccctggga aaaacccccg caagaggtta ggatcggggg ccgatggccg cccgcgaaga
     1141 cttgccgccg cctgtccagc gctggaacga gctcgtcgcc gcggcacgta tgcgctgcgc
     1201 ggtctgcggt gcgccctggc acgaggcggg cgagcgctgg cgcctcgtcg caacgggcga
     1261 cgaggcggag ccgcagcacg tgctcctctg ccccagctgc gccgcctgac tcccgccggg
     1321 tgacgacgac ggccagagct tgcggacgct ccctgaattg tggggctgca cccggccacc
     1381 gagtgcccga tcgtgtgtcg tcaggctgct gtgctctcga caccgccggt gatcggcagc
     1441 tcggagcttc cacggcgcac aacgagcatg agtgctgcga tgacgtccgg gtcgaactgt
     1501 gagccggcgc agcgctcgag ctctgcgagc gcctctggca gcgggagcgg ctcctggtgc
     1561 gggcgttgct cgaccatggc atagacggcg tcacacgcgg agatgatgcg cgatgggagc
     1621 ggtatctcat ctccggcgag ctgccccgga tagccctcgc cgtcccagcg ctcgtgggtc
     1681 gcgcgaatga cggctccgac gtgtgacagc gtcggcgagg ccgccagaat ccgctcgccg
     1741 acaagcgggt gctgcaggat gaactcccac tcccgctcgg tgagcgggcc ggccttgagc
     1801 aggatctcgt cgggcactgc gagcttgccg atgtcgtgga gctcggccgc gagttcgaga
     1861 tcggtgatct cgtcggcgcc aagcttcagc tcgcgcccga ccgccagggc cagctcgacg
     1921 acgttgcgaa cgtgctggcg aaggtctggc gcgcgctcgt agagggcctg caggagcggc
     1981 tcgtgcgttc gatagcgctc cgatcggcgt cggcgcttct cggcgtagag ccgctcgtcg
     2041 gcgaggcgta tcgccgccgg agcgtcactc gcctcgcccg ggagcgaaac cgctccgtac
     2101 gagcagttga tctcgaaacc ggcgcccttg gcacgcagcc cccggacggc ctcgtcgaga
     2161 atgactgccg tctcagcggg cccggcctcg gcgagaaggc agaactcgtc gccgcccagg
     2221 cgataggcga caccccggtc gccgaccgtc tgcgcgagct gctcaccgag ccgggcgagc
     2281 aagaggtcac cggcggggtg gccatacgag tcgttgtaga gcttgaagcc gtcgaggtcg
     2341 aagagtgcga ggacgtgggg ggcttcggaa gtcgcgcgct cgatcgcgtc gccgagatcg
     2401 gcgagcagct tccgcctgtt gggcagctcg gtcagcacgt cggtgtcggc ctccactctc
     2461 gtgcgggcca gcagcgagtc gatctgctgg aaggtcacga gcaaccggac gaccactgcc
     2521 gcgagcgcgc cggcggccag tgccacggcg tgaccgctga tcgagacgac cgagccggcc
     2581 gtcacgactg cgatggccac gatcgctccg ccgatcggca cggccggcac ggggcgcccc
     2641 tctgagccgt cgaccgcccg atcgtctgac caggcggcga gcgccacgag cacgagggca
     2701 gccggccaga ggaagtcgag gagggcgccc ggctggtaga cgtcgcccaa cgtctgcagg
     2761 acgtagaagc cgtccgcaag cgcgagcaca gcaaggccgc cacagagcag aagccaggtt
     2821 cgtctcgagc cacgggcagc gatgctgaag gcgccgacgg cgagggccag gagcacaacg
     2881 cccgccaccg ggtaggccag gcgagtcagg gtggctgcgg tgtcgctgtc gtctgcctcg
     2941 agaaccggct ggacgagaac cgcagctcca actgcggcgg cgctgagcgc gacgatcaga
     3001 ccgtcgagcc agaagcccgt ctcgacccgt cgcatccgcg accggagcaa gaggacgatg
     3061 ccgaggctgt agcagacgta cgtaccgagg tggccgacgt ccgcgaccga gggcagctcg
     3121 cgctccgccc gatcgagcgc gagtccgatg aacagctcgc ttccggccca caggccggtg
     3181 ccgagcccga acgcgagcca ggccgcccgc tcctcgcgta cggcgacggg ccggagcgcg
     3241 atcgtgacgg tggcggcgag caaggcgccg tagaagagca cggaaccagc gccgcgcagc
     3301 ggcactccgg ccgtggcgag cgctcccgag agcgcgagca gcgcgagcgc cgccggcgcg
     3361 caaatacgcg cgatcggtcc gaccgagggc ggcacgagag ccacgaaagc atgatcggct
     3421 cgtgctcgcc gtcacttgaa cgggggattg tgcgaatcgc gctgcgaggc cgcgcggtca
     3481 gtccagcgcc tgcggcggga tcacgtagcc gcgctcgagc gcaatggcgg ctcgctggtc
     3541 gtacacgtcc tcgtccagca ttcgccagcc gaaggcgtcg gcgagcttgt tgatcaagac
     3601 gaagctgacg cacacgtgga tcgcgtcctc gatcgcctcc tcgctcacgc ccgcttgccg
     3661 gactgcttcg gcgtccgcgg gcgtcacctc gccgggtctg agcgtgagct tctcgaggaa
     3721 gccgagcatc gcgcgcaggc cttcgctcac cggggccgtt cgccagtcgg cgacaaccgc
     3781 atcgaccagc tctttgttac cgagcgcccg cgccgcggtc gccgaatgct ggcctattca
     3841 ccacggacac tggttcagct tggagacgaa ggcggcgaag agctcgcgct ccccgacgct
     3901 ccactccgag tcgccccgcg agcaggcgtg aaggtagtcg gtgtacggct tgccgaagaa
     3961 ctcgggccga tagaggagcg tcgcggtcac gcccggtggc gcatcgatcc cgagcgcctc
     4021 tgtcatgtgc tcgagcgcgg caccgagaga agcgctcacc gtgatctcga agatctcgtc
     4081 ctccgagtag ccgtcgccct tcaggcgctc gacgtcggca tccacgacct tgtaggcgtg
     4141 caaggcgacc ttgtcgacgt acggccggag atcgtccggc accggcgttt cgggctcgcc
     4201 gaggcgaccc gcttcggcag atcgttgcgc cacagcctgc cgtagctccg cgctcgtctc
     4261 cgcggggccg gccaacgtcg tatttcgcag cttgtcgacc ttgtccgtgt acctgtcggc
     4321 caccgaagac tgacgctatc accttcgagg ggcgctgtac gctgtacggg ctcggagagg
     4381 tgaccgagcg gccgaaggtg cgcgactgga aatcgcgtgg gtgcctaaca cgtgcctcgc
     4441 gggttcaaat cccgccctct ccgtcgcagc ggggagctcc accggggctc cccgtacccg
     4501 cttcagctcc ctgacgcggt cgcgacgtag gccgcgacgt cggcaatctc gttctcgctc
     4561 aactggtcgg cgaacgccgg catcgcgccc ttcccgttcg tgaccctgtc gacgacgagg
     4621 tcatacgagg gctgcgtctc gtcgaggttc gggccgatgg cgccgttcga gccggcatct
     4681 gccagcgcgt gacacgcacc gcaccccgcg gaggcgtaga cgtcgcggcc ggccgccgga
     4741 tccccaacgg gtatcgccgg ctcggtctcg ctcgtctcag tctcggtcgt ctcggtctgg
     4801 gtcgtctcgg tctcggtcgt cgccgtctca gtcgtctcgg cctcggtcgt ctcggcctcg
     4861 gttcccggtt cggcaggcgc ggtctcggtc gccaccgcct cgtcaccgtg ctcctcggcc
     4921 tcctggccgg tcgcccacca gacggcgccg atctggacga tgaagagcgc cgccgtgatc
     4981 gtcaggaagg cggagagctt cttctcgccc gggaagtcct gcttgcgccg cggaatccac
     5041 atcgacacga cgagcgcgaa gacgatgaag ctgatcgcga cgatcagcag cacgaccttg
     5101 gcaccggtcg tcagcgagta gatcagcacg cgcgcaagct accacggcgc tccgcgcgca
     5161 cgtgagagga gctgcagcga ggtaaaggcg acttgtcgcc gaccgtttca ataggcttgc
     5221 gcgccatgtc agacgagcgg ctcgagcgct acgcgaggct tgcggtagag gtcggggtga
     5281 acctttcacc cggccaagac ctgcgcatct ctgctcaccc ggagcacctg ccgttggtgc
     5341 gggcgattgc caaggccgcg tacgagcgtg gcgcccacta cgtcgagacc acgtatggcg
     5401 acccgcacgt tctccaggcg cgtatcgccc atgcgccgga ggacgagctc ggctggtcgc
     5461 cgccgtggca ggtcgcgctg atcgaccacc tcgcggagga gcacggcgcc ttcatctcga
     5521 tcaccggcga cccggagccc gagctgctgg cagacctcga cggctcgcgc gtcgcgaagg
     5581 cgcgcccgaa gaagctcgcc gagcgtgtcc tcagcgcaac cggcgacggg cggatcgtct
     5641 ggacgatcat cgcgtgtccc accgccggct gggccgaggc ggtgttcggc tcaccggaca
     5701 ccgaacggct ctgggaggcc gtcgcggtga ccacccggct cgacgagccc gaccctgtcg
     5761 cctcctggcg cgagcacgtc gatcgactca cgacgcgtgc agccctgctc gacgagcgcc
     5821 gcttcgacgc gatccgcttc agcgggcccg gcaccgacct cacggtaggc ctgatgcccg
     5881 agtcgcgctg gatgaccgcc gccgaggaga gcgttcgcgg ccagtcgttc gtcgtgaaca
     5941 tgcccaccga ggaggtgttc accagtcccg accgacgtcg cgcagagggc gtcgtgcgcg
     6001 caacgatgcc gctcgcggtc ggggggcgca tcgtccgcga cctcgaggtg cgcttctcgg
     6061 ggggccgtgc ggtcgaagtg aacggatcga atggagccga cttcgtccga cagcagatgc
     6121 agacggacga gggtgcagcc tcgctcggcg agatcgcgct cgtggacggc gactcccgcg
     6181 tcggccagac gggcatcgtc ttcctcgaca cgctgttcga cgagaacgcc gcgtgtcaca
     6241 tcgcgtacgg cgacggcatc cgcaccgccg tcgagggggg catcgacctc gatccggagg
     6301 cgctgacggc gctcggctgc aacagctcga ccgttcacac ggatttcatg atcggaggcc
     6361 ccgaagtcgc cgtcgacgga atcgaggcgg gtggggctgc agtcccgatc ctgcgcgaca
     6421 acgactgggt gctctcgtag acagcgcgct ggctttcgta gcaatcgtca cgtaagctct
     6481 gggtgtgccg acggcctccc acgtgatgac ctcgcgactg gtgtccgtga gcacggaggc
     6541 aagcgccgcg gacgcggtgg ctcggatggt cgaggcaggc gtggggtcgg ttgtcgtcct
     6601 cgccggcagc cggctggagg gaatcttcac cgagcgcgac gttctcagcc tcgccgctgc
     6661 aggtgccgat ctcggggcga ccggtgtcgt ggacgtgatg acgcgcgcgg tcgtcacggc
     6721 cagcccggag gacgacccgg ttgcagtcgc ccacttgatg gcggacaaca acgtacggca
     6781 cctacccgtc acggtcgagg gtcacgttct gggcatcgtc ggcatccggg agatcctgcg
     6841 agttctgctc gagcgggcgt acgagagcca cgacaccgac gcgcacaaca cggcgcgaga
     6901 ccttctccgg cggccgtcgc tatcctctcc cgccgagggc gcatagctca gcgggagagc
     6961 gctcgcttca cacgcgagag gtccctggtt cgatcccagg tgcgcccatt cggtaccgcc
     7021 tgaccatgcg agaaccgctt taaacggcgt gtcaggtgtg ccagaggtgc cggaaatgac
     7081 cgaccaatgc gtaccccggt gcgtacctcg ggattcgcca gaaaagggga cggcgggccc
     7141 cgccgttgcc agcgtgcccg cccccctcgt cgtccccttc agtgacgctt ggcgggcccg
     7201 gcaagggtgg tgttatccgg cccgcctctt gctctcgttc tagtcggttt cctctatgtc
     7261 gctagtccag attcgggcaa ccggcgagga gcgggccccg ccctgaggag cccgcccctt
     7321 ctcttccccg tcccttttag gcggagctag gtgtcgcgag gccggttccc gagatcgcgc
     7381 atatgctctc ggggtgccgg tcgggcatga acgctctgta ggcgtacagc cgcagccgca
     7441 ccgtgccttc cgccgagagc acgtcgcgga agacctcgac gcgggcgacg ccctcgaaca
     7501 gcagggagtc gtccgtccgc acgacgtaga tctcgtcctc gttcgtgccg gcgccgtaga
     7561 gggtgccgac gttggagtcg agcacggcgc ggaggccggc gaacgactcg acgagccggc
     7621 gttttgcgag ccgaccgcct gcatcagcga gtcctgttgg aacagcggga acgagagctc
     7681 cttgccgagc caggccgccc gccgcggatg cacgatgatc gcgtcggccc gcggtagcgg
     7741 gtggtggcga ccgtctacac cccgtcgtac aacctcggca acagctccgc cccggtcggc
     7801 gtcgccgccg tgtaggtgac gctgccgatc gaggcgacgc cgcggatgcc ttggtgctgc
     7861 ccgttcgagc cgctacccga cagcagctgc ctgtccagga gcgagtcgta agcggctcgc
     7921 agatcgttga agacgaccgc gtccatgccc ggcatcgacc gctcgagcgc ctgcaaggag
     7981 agatcgacga agccgccgat cgtcgccacg ttggcggtca ggctgttgac gtcgggatcc
     8041 tgctcggtga gggtcgtgtt ctcggttgcc tgcacgccga cagcaccgcc ggtcgcgacc
     8101 ttcgtcaccg taatcgtcgt gcccgcgtcc ggcagcggcc tcgagccgat cgcgtcggcg
     8161 aacggcctgt ccggccgtgc cacctccgca tacaactcgc cgagatagac gggagggatc
     8221 aagccggcac cgc
//
LOCUS       DBNGNK010000288         3949 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_479727,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000288 DBNGNK010000000
VERSION     DBNGNK010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3949)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3949
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_479727"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1087
                     /locus_tag="AC150V_08505"
     CDS             <1..1087
                     /locus_tag="AC150V_08505"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3762998.1"
                     /translation="LAWSDISMIVARALQGVGAAIMTPAALSIIMTTFKEGADRNKAL
                     GVWGAVGASGATVGLIVGGVLADTIGWEWIFLLNVPIGLAVIGLTPFLIGESRKSTAT
                     RSFDIAGAVSVTAGIALLVYAIVDANGAGWGSAQTIGLIAVSVAALTLFVVIEWRSKS
                     PLMPLRIFRLRSLAGSNAAGFMFGGAVFGMFFLITLYLQQVLGFSPLEAGLAWLATSL
                     TVLVFAMVVQGVVTKIGPKLPLVSGLVVAAVGMWLLSRVPADGSYLSDLLLAMVVTGA
                     GMGMAFVSMSIGALEGVQERDAGLASGLINTSQQVGAALGVAPTCWEVLISRSVPLSA
                     WQCSPRLRCRGRRKSSPLSRRPRKLLR"
     gene            complement(1332..1760)
                     /locus_tag="AC150V_08510"
     CDS             complement(1332..1760)
                     /locus_tag="AC150V_08510"
                     /inference="COORDINATES: protein motif:HMM:NF025384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HIR3762999.1"
                     /translation="MSTTAGTGPLGQRRGVAFVVILSFITLGIYTLYWAYKTQEEMKQ
                     HTGEGLGGVLGLVIWIVLTIVTYFTIPSEVGNIYAKAGRPKPLTGWTGLWGLLPIIGW
                     IIWFVRVQGNLNRYWESVPAGGPAAQAAPPAAPESTPPAS"
     gene            complement(1830..2075)
                     /locus_tag="AC150V_08515"
     CDS             complement(1830..2075)
                     /locus_tag="AC150V_08515"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR3763000.1"
                     /translation="MAVPDGWIEVDQALQREFAFDDFVAALDFVNRVGELAEAENHHP
                     DIAISYNRVTLRWWTHTAGGVTDRDRDLAVKSAALAA"
     gene            2114..2959
                     /locus_tag="AC150V_08520"
     CDS             2114..2959
                     /locus_tag="AC150V_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3763001.1"
                     /translation="MAVTARRQAATSRLARSRKVRPGRELLVEYGFRPLADLVVRGLL
                     PLGVPPPAVVLANATAGFLAAAAIWQQEFVAGALLLQLKTLLDNADGQLARAAGKTSE
                     FGRYLDTVSDLVVNAAVFAALGAVTGRWWLALGSFVALSFVLSVDFNLEALYRRARQD
                     TPPSGEQEKGAAVSALRWIYRLLLAPQDRLITAFSESRLARLTGDGVDQELRRRATLA
                     YYDDATASVLANIGLSTQLAALGVFLLAGAPGGYVWVPLGCLAVLPAIQLRRELRVRR
                     VLRAP"
     gene            2956..3672
                     /gene="tmpR"
                     /locus_tag="AC150V_08525"
     CDS             2956..3672
                     /gene="tmpR"
                     /locus_tag="AC150V_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydropteridine
                     reductase/dihydrofolate reductase TmpR"
                     /protein_id="HIR3763002.1"
                     /translation="MSRGSALVTGSARGIGRGIVLALAAEGFDVAVHYRSSADDAEQT
                     RAEAAGLGVRAVALQGDVTDLDRAAALVGEAHSQLGSLEVLVNNVGDYVFKPLLELEA
                     QEWTRMLESNLSSAFATSQAAIPLMRAAGCGRIVNLGYAGAQHLVARPSLAAYGIAKT
                     GLILLTRAIAKIEAANGITANVVAPGVIENSKTQPLAEIPAGRVGRIDEVAAAVVYLV
                     SPEAEYVTGQVLEVAGGWNV"
BASE COUNT          541 a         1304 c         1392 g          712 t
ORIGIN      
        1 cctcgcctgg tctgacatct cgatgatcgt cgcccgcgcg ctccagggag tcggcgcggc
       61 gatcatgact ccggcggcgt tgtcgatcat catgacgacc ttcaaggagg gagcagaccg
      121 caacaaggcg ctcggcgtct ggggcgccgt cggtgcctcg ggcgcaacgg tcggactgat
      181 cgtcggtggt gttctggcgg acacgatcgg ctgggagtgg atcttcctgc tcaacgtgcc
      241 catcgggctg gccgtcatcg ggctcacgcc gttcctgatc ggcgaaagcc gcaagtcgac
      301 cgccacacgg agcttcgaca tcgccggtgc agtgtcggtg acggcgggaa tcgcactgct
      361 cgtctacgcc atcgtcgatg cgaacggggc gggctggggg tcggcgcaga ccattggcct
      421 gatcgcggtc tccgtcgccg cgctcaccct gttcgtcgtg atcgaatggc ggtcgaagtc
      481 gccgctgatg ccgcttcgca tcttccggtt gcgctcgctc gcgggctcga acgccgccgg
      541 cttcatgttc gggggagcgg tcttcgggat gttcttcttg atcacgctct acctgcagca
      601 ggtactcggc ttctccccgc tcgaggcggg actcgcctgg ctggcgacgt cgctgacggt
      661 gctggtcttc gcgatggtcg tccagggtgt cgtgaccaag atcgggccga agctgcctct
      721 cgtctcgggc ctcgtcgtgg cggccgtcgg aatgtggctc ctttcccgtg tgccggcgga
      781 cggcagctat ctgtccgatc tcctgctcgc catggtcgtg accggggcag gaatgggaat
      841 ggccttcgtc tcgatgtcga tcggtgctct cgagggcgtt caggagcgcg acgccggact
      901 ggcgtcgggg ctgatcaaca cctcccagca ggtcggtgcc gctctcggcg tggcaccgac
      961 ctgctgggag gtgttgatca gcaggtcggt gccgctctcg gcgtggcagt gctctcctcg
     1021 gttgcgctgt cgaggacgga ggaagtcctc tccgctgagc cggcgacctc gcaagctgtt
     1081 gcgctgaccg aggggttcca gtcggcgctc ctcgtcgcag ctgcgttcgc actcgtcgga
     1141 gcggtgctcg ccttcggcct catccggcgc gggggaccgg ctcatcagga gatcccggtc
     1201 gccggcggcg gcccgctcga gcccgccacc gccgaggcgg acagcgagcc tacggcgtag
     1261 agaaaacgtt cctcgagcga gaagagggcc ggcgccgccg gccctcttcg tctctcgtcg
     1321 cggcggtacg actaggaggc cggcggcgtg ctctccggcg cggcaggcgg cgcagcctga
     1381 gctgccgggc cccccgccgg aaccgactcc cagtacctgt ttaggttgcc ctgtacccgt
     1441 acgaaccaga tgatccagcc gatgatgggg aggaggcccc aaaggcccgt ccagccggtg
     1501 agcggcttcg ggcggccggc tttcgcgtag atgtttccca cctcggacgg gatcgtgaag
     1561 tacgtcacga tcgtgaggac gatccagatg acgaggccga gaacgccgcc gaggccctcg
     1621 cccgtgtgct gcttcatctc ttcctgggtc ttgtaggccc agtacagcgt gtagatgccc
     1681 agcgtgatga agctgagaat cacgacgaac gcgactccgc gtctctgtcc gagcgggccc
     1741 gtgccggcgg ttgtgctcat gatgcctcct ctgcttgggg tcgacctcgc ggctcgaacc
     1801 gtaatcgagt cggcggccgg accgggaagt caggccgcca gagcggcgga cttcacggcg
     1861 agatcgcggt cacggtcggt cactccgccg gcggtgtgcg tccaccagcg gagcgtcacc
     1921 cggttgtagc tgatcgcgat gtccgggtga tggttctcgg cctccgccaa ctcgcccaca
     1981 cgattgacga agtcgagcgc tgcgacgaag tcgtcgaagg cgaactcccg ctggagcgct
     2041 tggtcgacct caatccatcc gtccggcacc gccacggccg gagtatgacg cgtcagtacg
     2101 atcgtcggaa gcgatggccg tcacggcgcg tagacaggcg gcgacctcac ggctcgcccg
     2161 ctctcgaaag gttcgtcctg gacgcgagct tctcgtcgag tacgggttcc gtccgttggc
     2221 ggacctcgtc gtgcgcggtc tgctgccgtt gggcgtcccg ccgcccgcgg tggtgctcgc
     2281 aaatgcgacg gccggctttc tcgcggcggc tgcgatctgg cagcaggagt tcgtggcggg
     2341 ggccctgctg ctgcagttga agacgctcct ggacaatgcg gacggacagc tggcccgcgc
     2401 tgcagggaag acctccgagt tcggccgcta cctcgacacc gtgtccgacc tcgtcgtcaa
     2461 cgcggccgtc ttcgccgcct tgggcgcggt gacggggcgc tggtggctgg cgctcgggtc
     2521 gtttgtggcg ctcagcttcg tgctcagtgt cgacttcaac ctcgaggcgc tctaccgccg
     2581 cgcccggcaa gacacgccgc cgtccggcga gcaggagaag ggggccgcgg tgtcggctct
     2641 gcggtggatc tatcggctcc tgcttgcgcc gcaggatcgc ttgatcacgg cgttctcgga
     2701 gtcgcggctg gcgcggctga ccggggacgg cgtcgaccag gagctgcgcc ggcgggccac
     2761 gctcgcgtac tacgacgacg cgacggcgag cgtgctcgcg aacatcgggc tctccaccca
     2821 gctcgcagcg ctgggcgtgt tcctgctggc aggcgctccc ggcggctacg tctgggttcc
     2881 gctgggctgc ctcgcggttc ttcctgcgat ccagctccgg cgcgagctgc gcgtgcgccg
     2941 ggtgctccga gcgccatgag tcgcggctcg gcgctcgtga ccggatccgc ccgtggaatc
     3001 gggcgcggca tcgtgctcgc gctcgcggcg gagggcttcg acgttgcggt tcactaccga
     3061 tcgtcagcag acgacgcgga gcagaccaga gccgaggccg ccgggctcgg cgtccgggcg
     3121 gttgcgctac agggtgacgt gacggatctc gaccgcgccg ccgcactcgt cggcgaggct
     3181 cactcccagc tcggctcgct cgaggtgctc gtcaacaacg tcggcgatta cgtgttcaag
     3241 cccctgctcg agctggaggc gcaagagtgg acgcgcatgc tcgagagcaa cctgagctcg
     3301 gccttcgcga cctcgcaggc cgccatcccg ctgatgcggg ccgcaggttg tggccgaatc
     3361 gtgaatctcg gctacgcggg tgcacagcac ctcgtcgcgc ggccgagcct cgccgcctac
     3421 ggaatcgcca agacgggctt gatcctcttg acgcgtgcga ttgcgaagat cgaggctgcg
     3481 aacgggatca ccgcgaacgt cgtcgcgccg ggtgtgatcg agaactcgaa aacgcagccg
     3541 ctcgccgaga tcccggccgg tcgcgtcggc aggatcgacg aggtcgccgc cgccgtcgtc
     3601 tacctcgtct cgcccgaggc ggagtacgtg acgggtcagg tgctcgaggt cgccggcggc
     3661 tggaatgtct gaccagaggc cggaagcgcc gcgaggagca agtaacagac tgttacttgc
     3721 tcctttccgg cctctgatct cggccgctcg cgggcggtaa gtggcacgtt gttacttggc
     3781 ccttcgttcg aggagaggcg ggtgccgggt catgccgcgc cgcgaagagt cagccgctgg
     3841 ccgttcatcg acgcggcggc atcggacgac acgtaggcga tcgcgttcgc gatctcctcg
     3901 gccggcgtga acgtcgagaa gtccttgtcg ggctcggcgt cgcgcatct
//
LOCUS       DBNGNK010000289         2390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_407707,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000289 DBNGNK010000000
VERSION     DBNGNK010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_407707"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="AC150V_08530"
     CDS             complement(<1..1011)
                     /locus_tag="AC150V_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HIR3763003.1"
                     /translation="MTAARRRAATPTPLLIPYGGETLPLDVQVPCRVLEPNHVDARPP
                     EEILEEALSAPSGGESLQEFLARSPRVLVVVNDASRPTPTATVLDAIGPELARHPEVT
                     FIIATGAHRAPTDDEHQFIFGSHYATWKDRIVVHDARRDEDMTYLGQSTNGTEMYVNR
                     IVSEIGNVVTIGSVEPHYFAGFTGGRKSLLPGVASYRTIEMNHRLAMSARARAVTLEG
                     NPVNEDMVDAMRVLSGLRVFSIQTVLTGDHRLYAATAGDLHESFDAAIQHARDVFCVR
                     IDEKADLVVTVAHYPMDIDLYQSQKALDNGKLALQDGGIMILVSKCRSGVGDPAFLEL
                     LGR"
     gene            complement(1066..1932)
                     /locus_tag="AC150V_08535"
     CDS             complement(1066..1932)
                     /locus_tag="AC150V_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR3763004.1"
                     /translation="MAAVLLGALAGVLFGALAVAVRFGLGRGGDPEVGAVVILGVGAV
                     VAWLVAIPSLGSLGDVSSLWPFLLIGALVPGASQILFIFAVRDAGPARALIVIGIAPL
                     LSVGLALLLLSEPFRPLLLVATAFVVAGGLVLVSEQAKPVAYKRIGAIFAVLCAILFA
                     IRDNAVRWAAEDVEAPALLAAAVSLVGAVAVVAVYLALARPERVRSGIRAAMRAFWPA
                     GVALGFAYVCLIVAFDRGRVSVVAPLNATQSLWAIAFAAAFVGAAELIGRRTVLAGVL
                     VVAGAVIVGVIR"
     gene            2008..>2390
                     /locus_tag="AC150V_08540"
     CDS             2008..>2390
                     /locus_tag="AC150V_08540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3763005.1"
                     /translation="MNVRLAVAGACALGFALGWNVTNVGAVAEPLAGAYGVGLGVIGL
                     VATAFFITHVALQIPGGRASDRFGARLTALAGLAIAVCANGLALIAPVLGFAIAGRLV
                     VGVGTGIGFIAGSGYVRAVGGSPFA"
BASE COUNT          386 a          832 c          840 g          332 t
ORIGIN      
        1 tcggccgagc agctcgagga aagccgggtc tccgaccccg ctacggcact tcgagacgag
       61 gatcatgatg cccccgtcct gtagcgcgag cttgccgttg tcgagcgcct tctgcgactg
      121 atacagatcg atgtccatcg ggtagtgcgc gacggtcacg acgaggtcgg ccttctcgtc
      181 gatccgaacg cagaacacgt cgcgcgcgtg ctggatcgcc gcgtcgaagg actcgtggag
      241 gtcgcccgcc gtcgcggcat agaggcggtg gtcgcccgtc agcacggtct ggatcgagaa
      301 gacgcgtagc ccgctcagca cgcgcatcgc gtcaaccatg tcctcgttga cggggttgcc
      361 ctcgagcgtc accgcacggg cgcgggcgct catcgcgagt cggtggttca tctcgatcgt
      421 ccggtacgaa gcgacgcccg ggagcagcga cttccggccc cccgtgaacc cggcgaagta
      481 gtgcggctcg acgctcccga tcgtgacgac attcccgatc tcggacacga tccggttgac
      541 gtacatctcg gtgccgttcg tcgactgccc gaggtacgtc atgtcctcgt ccctccgcgc
      601 gtcgtggacg acgatccggt ccttccaggt cgcgtagtgc gagccgaaga tgaactggtg
      661 ctcgtcgtcg gtcggcgctc gatgggcgcc cgtcgcgatg atgaatgtca cctccggatg
      721 ccgggcgagc tcggggccga tcgcgtcgag caccgtcgct gtcggcgtcg gacgcgacgc
      781 gtcgttcacg acgaccaaga ctcgcggcga acgggcgagg aactcctgta gcgattcgcc
      841 gccggagggg gcagagagcg cctcctcgag gatctcttcg ggcggcctgg cgtcgacgtg
      901 gttgggctcg agcacgcggc acgggacctg cacgtcaagc ggcagcgtct cccccccgta
      961 cgggatgagc agcggtgtcg gcgtcgccgc tcgtcggcgt gcggcggtca tctccggagc
     1021 ctatcctggc cgaggatcac gaacgccggc gacgcccgac gcggctcagc ggatgacgcc
     1081 gacgatcacc gcgccggcca cgacgagcac tcccgccagg acggttcggc ggccgatcag
     1141 ctcggcggcg ccgacgaacg ccgccgcgaa cgcgatcgcc cagagcgact gcgtcgcgtt
     1201 gagcggcgcc acgacgctga cgcgtccgcg gtcgaaggcg acgatcaggc acacgtaggc
     1261 gaagccgagc gccacgccgg cgggccagaa ggcgcgcatc gccgcgcgaa tgccggagcg
     1321 gacgcgctct gggcgggcca gcgcgaggta gacggcgacg acagcgacgg cacccaccag
     1381 cgagaccgcc gcggccagaa gggccggcgc ctcgacgtcc tcggctgccc accgcacggc
     1441 gttgtccctg atcgcgaaca ggatcgcgca caggaccgcg aatatcgccc cgatccgctt
     1501 gtacgccacg ggcttggcct gctcgctcac gagcacgagc cctccggcga cgacgaatgc
     1561 cgtcgccacg agaaggagcg ggcggaacgg ctcgctcagg aggagaagcg cgagcccgac
     1621 cgagagcagc ggtgcgatcc cgatcacgat cagcgctcgc gccggcccgg cgtcgcggac
     1681 ggcaaagatg aagaggatct gcgaggcgcc cgggacgagc gccccgatga ggaggaacgg
     1741 ccagaggcta gacacgtcac cgagcgaacc aagcgaggga atcgcgacca gccaggcgac
     1801 cacggctccc actccgagga tcacgaccgc gccgacctcg ggatcgcccc cccggcccag
     1861 cccgaatcgc accgcgacgg ccagcgcgcc gaacagcacg cccgcgagcg cacccagcag
     1921 caccgctgcc atgactgtct aggctcctcc gatgacgcgg gcgcttggtg cgggcacgag
     1981 aacgatcatc cccgaagtgg gccgctgatg aatgtccgtc tggcagttgc cggcgcgtgc
     2041 gcactgggct ttgcactcgg ctggaacgtg acgaacgtcg gcgcggtcgc cgagcccctt
     2101 gctggcgctt acggggtagg gctgggcgtc atcggcctcg tggcgacggc gttcttcatc
     2161 acacatgttg cgctccagat cccaggcggt cgggccagcg accggttcgg cgcacggctg
     2221 acggcactcg cggggctcgc gatcgcagtg tgcgcgaacg gcctcgcgct catagcgccc
     2281 gtgctgggct tcgcgatcgc cggccgcctc gtcgtcggag tcggaaccgg gatcgggttc
     2341 atcgccggca gcggctacgt ccgcgcggtg ggtggatcgc cgttcgcgca
//
LOCUS       DBNGNK010000290         6408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_765873,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000290 DBNGNK010000000
VERSION     DBNGNK010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_765873"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /gene="hisIE"
                     /locus_tag="AC150V_08545"
     CDS             complement(<1..556)
                     /gene="hisIE"
                     /locus_tag="AC150V_08545"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013268453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR3763006.1"
                     /translation="MAGPQLTPVIVQDATTDRVLMLAYADDEALRRTRATGEAWFWSR
                     SRGEYWRKGATSGNTMAVEEIRDDCDGDAVLYRVRPAGPACHTGSESCFAPWLWRVVA
                     ERARERPEGSYVAGLLAAGAAGAARKVGEEGVEAALAGAAESDQRLIEELADLWFHSY
                     VLLAARGLDPARVEEELKRRAAPPG"
     gene            complement(538..1278)
                     /gene="hisF"
                     /locus_tag="AC150V_08550"
     CDS             complement(538..1278)
                     /gene="hisF"
                     /locus_tag="AC150V_08550"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR3763007.1"
                     /translation="MVKKRVIPCLDVAGGRVVKGVRFENLRDMGDPIELATRYSELAA
                     DELVFLDITATVENRGPILRLVEAAAEELTIPFTVGGGVTALEDARGLLRAGADKVGV
                     NRAAFDDPSILSALADEFGSQAVVCAIDARAGEVVTHAGRTPRGRDAVEWAVEAVERG
                     AGEILLTSIDADGTRAGYDLELTASVAAAVEVPVIASGGAGEAKHLAEAFEVGAEAAL
                     IASIVHERPDRLPELKSELGEMGWPVRS"
     gene            complement(1260..1859)
                     /gene="hisH"
                     /locus_tag="AC150V_08555"
     CDS             complement(1260..1859)
                     /gene="hisH"
                     /locus_tag="AC150V_08555"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR3763008.1"
                     /translation="MKVVLADYGAGNLRSVCAAFSRAGAAPVVTTDATELREAPLAVI
                     AGVGHVESAARGLATGGLDEALRDRFANDRPTLGICVGLQLLFEASEEGGKGLGLLTG
                     PVVRLRARRVPHMGWNELRATNSSAVLDGLDGADVYFAHSFAAAPTGDVTVAVVEHDG
                     QVVAAVESGAVAGVQFHPERSGAAGAHLLENVLAWSRNV"
     gene            complement(1856..3481)
                     /locus_tag="AC150V_08560"
     CDS             complement(1856..3481)
                     /locus_tag="AC150V_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3763009.1"
                     /translation="MIRRSGREAVTNESSTAAEALALPLPENFRHYVWAPTTADIAER
                     HRLPTEAVLRFDQNTPSLPGVPQIPLAESFARLNEYPDGRYVDLRGAAAGYSGLQAEN
                     VVIGAGADDLIGVVARTFLSPGRRAAIDEPTYSLYRIVTGLQRAEAVGADDEADVVWI
                     CNPNNPTGEAVEPELIVERARERKDSVVVVDEAYVEFGARSVAPWVDELPNLIVLRTL
                     SKAFGFAALRVGYALARRDTAELLEQRRAPAPVSAPAARIAAAALRRPSFELAELAAE
                     RERVRGALIAAGLEVLPTVTNFVYLRGSESLADPLEARGLVVRRVPGGIRITLRTPPE
                     NDVILAALGARVPPRDGRSATVVRTTTETAIRLTLDLDGAGRVRVATGIGFLDHLLTL
                     AAFHAGFDLELLAGGDLSVDEHHTVEDVLAALGAALAEALREREGVARYGSATVPMDE
                     ARATAAVDLVRRPHAEIALVFTGDRVGALAPSLLPHALERFAVEAGCTVHVEAAGKDD
                     HHVAEAAFKALGQALRQACAPGGAGIRSTKGAA"
     gene            complement(3478..4656)
                     /gene="hisD"
                     /locus_tag="AC150V_08565"
     CDS             complement(3478..4656)
                     /gene="hisD"
                     /locus_tag="AC150V_08565"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR3763010.1"
                     /translation="MSAASLDEALVAVTPIVADVRGRGDAALIEWTERFDGSRPEGVR
                     VRPEQIERAQVDDEVLDALRRMIAAVRTFNDAQWPRDLALEAAPGVRSERRWRPLDSV
                     GICVPGGRSPLPSSLVMCAVPALVAGVPRIAVITPRPADVTLAVARELGIDEIYAVGG
                     AQGVAALAYGTESIAPVAKIVGPGSLYVTAAKLLVSTRVGIDLPAGPSEVVVIADASA
                     SPRACAANLAAQAEHGADSAALLLTDDPALSDAVATLVVDYDNVTCEVVESLDEALER
                     SEGIAPEHLELLVADPFPLLERVRNAGSVFVGAPAVLGDYAAGATHVLPTGGLARSSG
                     GLSLTDFLKPLQIVRATPEGLRGARAVVGPVARVEGLPLHAAALDRAVEALDGTEASA
                     "
     gene            complement(4653..5537)
                     /gene="hisG"
                     /locus_tag="AC150V_08570"
     CDS             complement(4653..5537)
                     /gene="hisG"
                     /locus_tag="AC150V_08570"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR3763011.1"
                     /translation="MSGFTRAGENGRLTLAVPAKGRMAEPSLKLCLDAGLSFEATERS
                     LVVPCANAPVDLLLVRPSDIPEYVQDGVVDCGITGANLVHEADADVVSLAELGFARCT
                     LEAAVPEDAPQKEIGELDGLRIATAYPASTRRLLADRGVSCELVPVSGSVEATPRLGL
                     ADAIVDLVSTGSTTSANGLRRIGSLLSSQAVLVGGRDALERRSEAIDQLELMLSGVVA
                     ARRRRYVMMNATVDSLPLIRSLLPSMGAPTVLPLADDGEIAVHAAVDADDVWQLLPEL
                     KQAGASSILVVPVERLVP"
     gene            complement(5534..5863)
                     /locus_tag="AC150V_08575"
     CDS             complement(5534..5863)
                     /locus_tag="AC150V_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HIR3763012.1"
                     /translation="MAKRNRADDIAEREIEPIPQLDSLADAFLALEDRDEVRRFLRDL
                     CTLPELEALTHRWQTAQLLEKGVPYVEIAERVPTSTATVTRVAQWLRHGAGGYRIALD
                     RAGGARR"
     gene            5888..>6408
                     /locus_tag="AC150V_08580"
     CDS             5888..>6408
                     /locus_tag="AC150V_08580"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR3763013.1"
                     /translation="MLARNTAFEVIPAIDVLEGRVVRLSQGRREKVTIEGGDPVSLAE
                     RYAAEGAVRLHLVDLDGAFVGKPSSGLLARIVAATPLPVQVGGGYRTLEAIDAAVADG
                     ADRVMVGTSALAPGFLEAATERFGDRLVVAVDVKDGRVAVDGWTTSSSLDPEALVQRC
                     VEAGVARLLVTST"
BASE COUNT          999 a         2287 c         2204 g          918 t
ORIGIN      
        1 cccccggggg ggccgcgcgg cgcttcagct cttcctcgac gcgcgccggg tcgagaccgc
       61 gcgcagcgag cagtacgtag ctgtggaacc agaggtccgc gagctcctcg atcagccgct
      121 gatccgattc cgcggctccg gccagggccg cctctacccc ctcctcaccg accttgcggg
      181 cggcgccggc tgcaccggca gcgagaaggc cggcgacgta ggagccctct ggtcgctcgc
      241 gcgcgcgctc ggcaacaacc cgccacagcc atggtgcgaa gcacgactcg gatccggtgt
      301 ggcaggccgg tcccgccggt cggacacggt agagcaccgc gtcaccgtcg caatcgtcgc
      361 ggatctcctc gacggccatg gtgttgccgg aggtcgcgcc cttacgccag tactcaccgc
      421 gcgagcggct ccagaaccac gcctcgccag tggcgcgcgt ccgccgcagc gcctcgtcgt
      481 ctgcataggc gagcatcaag acgcgatcgg tcgtcgcgtc ctgaacgatc accggcgtca
      541 gctgcggacc ggccatccca tctctcccaa ctccgacttg agctccggca ggcggtcggg
      601 gcgctcgtgg acgatggacg cgatcagagc ggcctccgcg ccgacctcga aggcctctgc
      661 gagatgcttt gcctcccccg cgccaccgga cgcgatgacg ggcacctcga ctgcagccgc
      721 aaccgaggcc gtcagctcga ggtcatagcc cgcccgcgtg ccgtcggcgt cgatcgaggt
      781 gagcaagatc tcgccggcgc cacgctcgac cgcctcgacg gcccattcga ccgcgtctcg
      841 accgcgggga gttcggccgg cgtgtgtcac gacctcgccg gcgcgcgcgt cgattgcgca
      901 cacgaccgcc tgcgacccga actcgtcggc gagcgccgag aggatggacg gatcgtcgaa
      961 cgccgcgcgg ttgacgccga ccttgtcggc tcccgctcga agcaatccgc gggcgtcctc
     1021 gagggccgtc acgccgcccc cgacggtgaa gggaatcgtc agttcctcgg cggccgcctc
     1081 gacgaggcgg agaatcggcc cacggttctc gaccgtggcc gtgatgtcga ggaagacgag
     1141 ctcgtcggcg gcgagctccg aataccgggt cgcgagctcg atcggatcac ccatgtcgcg
     1201 gaggttctcg aagcggactc ccttgacgac ccggccgcct gccacgtcca ggcagggaat
     1261 cacacgtttc ttgaccatgc cagcacgttc tcgaggaggt gagcacccgc tgcgccgctg
     1321 cgctccggat ggaactgcac tcccgcgacg gcgccggact cgaccgcggc aacgacctgg
     1381 ccgtcgtgct cgacaaccgc aaccgtgaca tcaccggtcg gagcagcggc aaagctatgc
     1441 gcgaagtaga catcggcacc gtcgaggccg tcgagcacgg cgctcgagtt cgtcgcacgc
     1501 agctcgttcc agcccatgtg cggcacgcgc cgggcgcgca gtcggacgac cggaccggtg
     1561 agcagaccca atcctttgcc cccctcctcg ctcgcctcga agaggagctg gagcccgacg
     1621 cagataccga gcgtggggcg atcgttcgcg aaccggtcgc gcagcgcctc gtcgaggccg
     1681 ccggttgcca ggccacgagc cgcggactcg acgtgaccga cgcccgcgat cacggccagc
     1741 ggagcttccc ggagctcggt cgcgtcggtg gtcacgaccg gtgccgctcc ggccctcgag
     1801 aaggccgcgc agacgctgcg caggttgccg gcgccgtaat cggcgagcac gaccttcacg
     1861 ctgcgccctt cgtcgatctg atgccggcgc cgccgggagc gcacgcttgc cgcagcgcct
     1921 ggccgagcgc cttgaacgcg gcttcggcga catggtgatc gtcctttccg gccgcttcga
     1981 cgtggacggt gcagccggct tcgacggcga accgctcgag ggcgtggggc aagaggctcg
     2041 gcgcgagcgc cccgacgcga tcccccgtga agacgagcgc gatctcggcg tgcggtcggc
     2101 ggacgagatc gacggccgcg gttgcccgcg cttcgtccat cggcaccgtt gccgatccat
     2161 agcgagcaac tccctcgcgc tcgcgcagcg cttcggcgag cgcggcaccg agtgccgcga
     2221 gaacgtcctc cacggtgtga tgctcgtcga cgctcaggtc gccgccggcc aagagctcga
     2281 gatcgaagcc ggcgtgaaag gcggcgaggg taaggaggtg gtcgagaaag ccaattccgg
     2341 tggcgaccct cacccgcccc gcaccgtcga ggtcgagggt cagccgaatc gcggtctcag
     2401 tcgtcgtccg cacgacggtc gccgatcgac catctcgcgg cggcacccgg gcgccgaggg
     2461 ccgcgaggat gacgtcgttc tcgggcggcg tccgcagcgt gatccggatg ccgccgggca
     2521 cgcgccggac tacgaggccc cgcgcctcta gcggatccgc gagactctcc gagccgcgga
     2581 ggtagacgaa attcgtgacc gtcggcagca cctccaaacc ggcggcgatg agcgctcctc
     2641 gaacacgctc gcgctcggct gcgagctcgg cgagctcgaa gctggggcgg cgcaacgctg
     2701 cggcggcgat acgcgccgct ggggccgaca caggcgcagg cgcccgtctc tgttccagca
     2761 gctccgcagt atctcgccga gccagcgcat agcccacgcg cagcgcggcg aagccgaagg
     2821 ctttggagag cgttcgcagc acgatcaggt tgggcagctc gtcgacccac ggggccacgg
     2881 agcgagcgcc gaactcgacg taggcttcgt cgacgacgac gaccgagtct tttcgctcgc
     2941 gcgcgcgctc cacgatcagt tccggctcga ctgcctcacc cgtcgggttg ttgggattgc
     3001 agatccacac gacgtcggcc tcgtcgtccg cgccgaccgc ttccgcacgc tgcaagccgg
     3061 tcacgatcct gtacagcgaa taggtcggct cgtcgatggc agcccggcgt ccgggggaga
     3121 gaaacgtccg cgcgacgacc ccgatgagat cgtcggcgcc ggcgccgatc acgacgttct
     3181 ctgcctgtaa gccggagtag ccggcagccg cgccgcgcag gtcgacgtag cgcccgtccg
     3241 gatactcgtt cagccgagcg aagctctcgg cgagcgggat ctgcggcacc ccggggagcg
     3301 agggcgtgtt ctggtcgaaa cggagcacgg cctcagtcgg cagacggtgg cgctccgcga
     3361 tgtcggccgt cgtcggcgcc cagacgtagt gacggaagtt ctccggcagg ggaagggcga
     3421 gcgcctccgc ggcggtactc gactcgttcg tgaccgcctc gcgaccgctg cgccggatca
     3481 tgcggatgcc tccgtgccgt cgagcgcctc gacggcgcga tcgagcgcgg ccgcgtgcag
     3541 aggcagtcct tcgacgcggg cgaccggccc gacgacagct ctcgcgccac gcagtccctc
     3601 gggggtcgcg cggacgatct gcaagggctt caggaagtcc gtgaggctga gccctccgga
     3661 ggagcgcgcg aggccgcccg tcggcagcac gtgcgtcgcg cctgccgcgt agtcgccgag
     3721 gacggccggt gctcccacga agaccgaccc ggcatttcgg acacgctcga gcagtgggaa
     3781 gggatccgcc acgagcagct ccagatgctc gggagcaatt ccctcggagc gctccagagc
     3841 ctcgtcgagc gactccacca cttcgcacgt gacgttgtca taatcgacaa caagagtggc
     3901 aaccgcgtcg gacagagccg gatcgtcggt caggagcagc gcggcgctgt ccgcgccgtg
     3961 ctccgcctgc gccgcgagat tggcggcaca ggctcgaggt gaagcggacg cgtcggcgat
     4021 caccacgacc tcgctggggc cggccggcag atctatcccc acgcgcgtcg agaccagcag
     4081 cttggccgcc gtcacgtaga ggctgcccgg cccgacgatc ttggcgacgg gggcgatgct
     4141 ctccgtcccg tatgcgagcg ccgcgacccc ctgcgcaccc cccaccgcgt agatctcatc
     4201 gatcccgagc tcgcgtgcca ctgcgagcgt gacatcggcg gggcgcggcg tgatgacggc
     4261 gatccgcggg actccggcta cgagcgccgg gaccgcgcac atcaccagcg aggacggcag
     4321 cggcgagcgg ccgcccggga cgcagatccc aacggagtcg agtggtcgcc agcgtcgctc
     4381 cgaacgaacc cccggagcgg cctcgagtgc cagatcacgg ggccactgcg catcgttgaa
     4441 cgtccgcacc gccgcgatca tgcgacgcag cgcgtcgagc acctcgtcgt cgacctgggc
     4501 gcgttcgatc tgctcgggac gcactcggac accctccggc cgagatccgt cgaagcgttc
     4561 cgtccattcg atcagagcgg cgtcgccgcg cccgcggacg tccgccacga tcggtgtcac
     4621 ggcgacgagc gcctcgtcga gcgaggcggc gctcacggga ccaggcgctc gaccggaacg
     4681 acgagaatcg acgaagcgcc cgcctgtttc agctcgggca ggagctgcca gacgtcgtcg
     4741 gcgtcgacgg ccgcgtgcac ggcgatctca ccgtcgtcag ccaaaggcag caccgtcggc
     4801 gcccccatgc tcggcagcag cgatcggatc agcggcaggg agtcgaccgt cgcgttcatc
     4861 atcacgtagc gtcgtcggcg cgcggcgacc acgcccgaga gcatgagctc gagttgatcg
     4921 atcgcctccg atcgacgctc cagcgcgtcg cgtccgccga cgaggacggc ctgcgacgac
     4981 aggagcgacc cgatccgccg cagcccgttc gcactggtcg tcgatccggt ggaaacgagg
     5041 tcgacgatcg cgtccgcaag accgagcctt ggcgtggcct ccaccgagcc ggacaccgga
     5101 accagctcgc aagagacgcc gcggtcggcg aggagtcgcc gggtcgacgc cggatatgcg
     5161 gttgcgatcc ggagcccgtc cagctcaccg atctccttct ggggcgcgtc ctcgggaaca
     5221 gccgcttcga gcgtgcaccg cgcgaagccg agctcggcga gcgagacgac gtcggcatcg
     5281 gcttcgtgca cgaggttcgc gccggtgatc ccgcagtcca cgacgccgtc ctgaacgtac
     5341 tctgggatgt cgctcggccg cacgagaagg aggtcgaccg gggcgttcgc gcagggcacc
     5401 acgagcgagc gctcggtcgc ctcgaagctc agaccggcgt ccaggcacaa cttcagcgac
     5461 ggctcggcca tgcgcccttt cgccggcacg gcaagcgtca gccggccgtt ctctcctgcc
     5521 ctcgtgaacc cgctcatcgt cttgcacctc cagcgcgatc gagggcgatc cggtagccgc
     5581 cggccccgtg ccggagccat tgcgctacgc gggtcacagt cgcggtcgag gtcggcaccc
     5641 gctctgcgat ctcgacgtag ggaaccccct tctccagaag ctgcgcggtc tgccagcgat
     5701 gggtcagcgc ctcgagctcc ggcagcgtgc acaggtcgcg gaggaaccgt cgcacctcgt
     5761 cccgatcctc gagagcgaga aaggcgtcgg cgagagagtc gagctgcgga atcggctcga
     5821 tctcgcgctc ggcaatgtca tctgctctgt ttcgcttcgc catgtgctcg catggtagca
     5881 tgttaacgtg ctagcacgca atactgcttt cgaagtcatt cccgcgatcg acgtgctcga
     5941 gggccgagtc gtccggctgt cacaaggccg ccgcgagaag gtcacgatcg agggcggaga
     6001 cccggtctcc ctggccgagc gttacgcggc ggagggtgcc gtacggcttc atctcgtaga
     6061 tctcgacggc gctttcgtcg ggaagccatc gtcgggccta ctggcgcgca tcgtggctgc
     6121 aaccccactg ccggttcagg tgggaggcgg ctacaggacg ctcgaagcga tcgacgccgc
     6181 cgtggccgac ggggccgacc gcgtcatggt cggaacgtct gccctggctc ctggcttcct
     6241 cgaggcggca accgagcgtt tcggagatcg gctcgtcgtc gccgtcgacg tcaaggacgg
     6301 ccgagtggcc gtcgacggct ggacgacgtc gtcgtcgctc gacccggagg cgctcgttca
     6361 gcggtgcgtc gaggccgggg tcgcgcggct gctggtgacg tcgaccag
//
LOCUS       DBNGNK010000291         4419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_408082,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000291 DBNGNK010000000
VERSION     DBNGNK010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4419
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_408082"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..703
                     /locus_tag="AC150V_08585"
     CDS             314..703
                     /locus_tag="AC150V_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763014.1"
                     /translation="MIIALTHAQEDAVLGAMRAIALAHGAAGVSAEDRATVIAAGRIV
                     LGRSEVEPDRIAPVAPLGLAAAMLSDDDARMACRMLAVMSLVDGRIDSDKVGLVQDFA
                     AELGVGEDYLKSWPRRRRTRSPPPPPA"
     gene            700..1227
                     /locus_tag="AC150V_08590"
     CDS             700..1227
                     /locus_tag="AC150V_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763015.1"
                     /translation="MIRKNAESFPELDHSRIDEAPLAAFLPYREGHDDPGLEARYLEL
                     EHAAPETFGRAFFDHFQSNGFRFPGHPDGLAEGFTTAHDTSHVLGGYSTGQQGELCVS
                     TFIGAMHPDHPMAAEVLPVIFSWHLGIKLNELAKSHTGPSIPAGSGRPGTAAPPPRWT
                     WRTPTGTSGPPLTSP"
     gene            1389..>4419
                     /locus_tag="AC150V_08595"
     CDS             1389..>4419
                     /locus_tag="AC150V_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3763016.1"
                     /translation="MPGDTSRFVGGPPARLEVRLLGAVEVVLDGRRRRAFSSLRLQRL
                     LALLALRSDLQHRSRLAFELWPDSAEGQARTNLRKLLHDLRRALPDVDEFVEIDNETV
                     VLTPTGPTDVDVWRFHDAVAAGDLELAARLYSGDLLPACYDDWVLAERARLRAEAHAA
                     LVRLAEGAAARGDHDATIRHAQRIIDLEPTDEVAVRMQMHAHLALGDRAAALRSYHRC
                     AEGLERELAVPPGEALRALYRQLRADTLDGAEPRGEDPAPVTESPFVGRGLELNRLDQ
                     AWRAAREGGAHLVVVTGEPGIGKSRLALELARRVRAEGHVVASARAYEAAGGLPWGPV
                     VDLLRSDALRSHIDTLDAVWRNELARLLPELHDPSRPPEPSPSGDLARRHRLFAAVSR
                     AVIGDRPRLMIIDDLQWCDAETIELIGYVVRSGRTAPVLIIGTLRSEEIPDHHPLVAL
                     VDALARDDAVTTVPLERLDEATTATLAARLGADGTVDPELAARLWSETEGNPLFVVEA
                     LRAGISPDGARPVLTPTMRAVLRARLSQLPDAARRLAEVAAVIGRPFSVGLMAAATGI
                     GEGELVDHLDELWHRRILRDQGLAYDFSHDKLRAVALEMVGPARRRRLHRAVAEAIAV
                     EFRDDIDAAGPQLAAHYDRAGMVEPAIDAYCTAGARAVAVSALDDAVTMFGRALSLLA
                     EVSPSPDRDALELDIRIALGSPLVAIDGYGSTGAHRLYARALALCRKLDRPVNPAILR
                     GLGLARLQGCRFGECSELGQALLDEEGHDPVAGTEGRYLLGVSAFWRGDLARARDQLE
                     GAIAAYDVSHRDEHLASYAQDPKAVCLVRLALVELWAGDAGRADETARSALEIAVDLD
                     HLMTLWYVNTYASILAAESEDLARLAELLGDAVRLRQRLPMRYLMIVGAALRGWLDVC
                     EGSTGGIEKIERSVAHSRTDGETLHLTYCLLLLARARGVVGELREGRAATREGLSWSH
                     SRDQRYLEAELWRVDGELAYRGGDGEAAAASLRSAIDV"
BASE COUNT          611 a         1589 c         1558 g          661 t
ORIGIN      
        1 ggccgcctca atcgactcgg cgagatcctc ggccgcctct agtccgcgac gggggctggg
       61 ggtcacggcg gggtcacagg accccccggc atccggcggt agtcgcggcg cccgtccgac
      121 gccgagatcc cgcatccgga ggacggagct gtactcggcg gcactcaccg ggctcaactc
      181 ataatccctg ggtcgcaggt tcgagtcctg ccgcgcccat cgacatagcc ggccgcacgc
      241 gcatccgggg cggggtctcc tcgggtcgta ccgcggcggc cggggcggac cgggaagacg
      301 ctagcgtggc cccatgatca tcgcgctgac gcatgcgcag gaggatgccg tcctcggggc
      361 gatgcgcgcg atcgccctcg cgcacggcgc cgcgggggtc agtgccgagg atcgggcgac
      421 cgtcatcgcc gcgggccgga tcgtcctggg acgcagcgag gtcgagccgg accgcatcgc
      481 accggtcgcg ccgttgggcc tggcggccgc gatgctgtcc gacgacgacg cgcggatggc
      541 gtgtcggatg ctcgcggtga tgtcgctcgt cgacgggcgc atcgactccg acaaggtcgg
      601 gctcgtgcag gacttcgctg ccgagctcgg ggtgggcgag gactacctca aatcctggcc
      661 gaggcggcgc aggacgagat ctccgccgcc gccgcctgca tgatccgcaa gaacgccgag
      721 agcttccccg agctcgacca cagcaggatc gacgaggctc cgctggccgc ctttctgccc
      781 taccgggagg gccacgacga ccccggtctc gaggctcgct atctcgagct ggagcacgcg
      841 gcgccggaga ccttcggccg ggccttcttc gaccacttcc agagcaacgg gttccgcttc
      901 ccgggtcacc ccgacgggct cgccgagggg ttcacgacgg cgcacgacac ctcgcacgtg
      961 ctcggcggct actccaccgg ccagcagggc gagctgtgcg tgtccacctt catcggcgcc
     1021 atgcaccccg accacccgat ggcggcggag gtgctcccgg tcatcttcag ctggcacctc
     1081 gggatcaagc tgaacgagct cgcgaagagc cacacgggcc cttcgatccc cgccggttct
     1141 ggacggcctg ggaccgcggc gccgccaccg cggtggacgt ggcggacccc gactgggact
     1201 tctgggccgc cgttgacgtc cccctgagcg agctgcgacg cgcctgccac gtccccccgg
     1261 tcgatccgga gctcctggcc tgacgccgcc cttcgccgga cgcgcgccgg gcggggaccg
     1321 gctcgtgatc gacaccccgg cggggaggga gcagactccc ggtgtgggac ctcgatcgga
     1381 ggagccacat gccgggcgac acaagcaggt tcgttggcgg accgccggcg cggttggagg
     1441 tccggctcct cggtgctgtc gaggtcgtct tggatgggcg gcgtcgtcgc gcgttcagct
     1501 ctctgcgcct gcaacgtctg ctggcgttgc tggcgttgcg gagtgacctc cagcaccgtt
     1561 cgcgactcgc gttcgagctc tggcccgact ccgccgaagg tcaggcccga accaacctga
     1621 gaaagctgct ccacgacctc cgtcgagcgc ttccggacgt cgatgagttc gtcgagatcg
     1681 acaacgagac cgtggtcctg actccgaccg ggccgaccga cgtcgacgtg tggaggttcc
     1741 acgacgccgt ggcggccggt gatctcgagc tggccgcgcg cctctattcg ggtgatctcc
     1801 tccccgcgtg ctacgacgac tgggtcctgg ccgagcgagc gaggctgcga gccgaggccc
     1861 atgccgccct cgtgcgactg gcggaagggg ctgccgcgcg gggcgaccac gacgccacca
     1921 tcaggcacgc ccagcggatc atcgacctgg agccgaccga cgaggtggcc gtgcgcatgc
     1981 agatgcacgc gcatctcgcg ctcggcgaca gggctgcggc gctgcgctcc taccaccggt
     2041 gcgcggaggg gctcgagcgc gagctcgccg tgccgccggg cgaggcgctc agggccctgt
     2101 accggcagct ccgggcggac accctcgatg gcgcggaacc gcggggcgag gacccggcac
     2161 ccgtcaccga gtcgccgttc gtgggccgcg ggctcgagtt gaaccggctc gaccaggcat
     2221 ggcgtgccgc tcgggagggt ggggcacacc tcgtggtggt gaccggggag ccgggtatcg
     2281 ggaagagccg cctcgcgctg gagctcgccc gccgcgttcg agcagaggga cacgtggtgg
     2341 cgtccgctcg tgcgtacgag gctgcgggcg gactgccgtg gggtcccgtc gtcgacctcc
     2401 tccgatcgga tgcgctccgc agccacatcg acacgctcga cgcggtctgg aggaacgagc
     2461 tcgctcgtct cctccccgag cttcacgacc cctcccggcc acccgagccg agtccgtcgg
     2521 gagacctggc ccggcgccat cggctcttcg ctgcggtgag ccgcgccgtc atcggcgacc
     2581 gaccacggct gatgatcatc gacgacctgc agtggtgtga cgccgagacc atcgagttga
     2641 tcgggtacgt cgtccgttcc gggcggacgg ctccggtgct gatcatcggc accctccgct
     2701 ccgaagagat cccggatcac catcccctcg tcgcactggt cgacgcgctg gcccgtgacg
     2761 acgcggtgac caccgtcccg ctcgagcggc tcgacgaagc caccaccgcg acgctcgcgg
     2821 ctcggctcgg cgcggacggc acggtcgatc cggagctggc ggcgcgactc tggagcgaga
     2881 ccgagggcaa cccgctcttc gtcgtcgagg cgcttcgagc cgggatctcc cccgacggag
     2941 ctcgaccagt gctcactccg acgatgaggg cggtgctgcg ggcccgcctg agccagctcc
     3001 ccgacgcggc tcggagactc gccgaggtgg cggcggtcat cggccgaccc ttctcggtgg
     3061 gtctgatggc cgcggccacc gggatcggcg agggcgagct cgtcgatcac ctcgacgagc
     3121 tctggcatcg gcggatcctc cgcgatcagg gcctcgccta cgatttcagt cacgacaagc
     3181 tacgggcggt cgccctggag atggtcggtc cggctcgtcg acgccgactc caccgggccg
     3241 tcgccgaggc gatcgccgtc gagttccgcg acgacatcga cgcggccggc ccgcagctcg
     3301 ccgcgcacta cgacagggcc ggcatggtcg aaccggccat cgatgcctac tgcacggcgg
     3361 gagcccgagc ggtcgcggtg tcggctctcg acgatgcggt caccatgttc gggcgagcgc
     3421 tctccctgct cgccgaggtg tcgccctcgc cggaccgcga tgcgctcgag ctcgacatcc
     3481 ggatcgcgct cgggtcaccc ctggtggcca tcgatggcta cggctccaca ggtgcgcacc
     3541 gcctctacgc gcgcgccctc gcgctctgcc gcaagctcga ccggcccgtg aacccggcga
     3601 tcctccgcgg tctcggcctc gcccggctcc agggctgccg cttcggcgag tgcagtgaac
     3661 tcggccaggc cctcctcgac gaggagggcc acgatccggt cgccgggaca gagggccgat
     3721 atctcctcgg cgtgagcgcg ttctggcggg gtgacctggc gagagcccgc gaccaactcg
     3781 aaggcgccat cgcggcctat gacgtgtccc atcgtgatga gcatctggcg tcgtacgccc
     3841 aggacccgaa ggcggtctgc ctcgtccggc tggccctcgt cgagctgtgg gccggagatg
     3901 ccggccgggc cgacgagaca gcccgatctg ctctcgagat cgccgtcgat ctcgatcatc
     3961 tgatgacgct gtggtacgtc aacacgtacg cgtcgatcct cgcagccgaa tccgaggatc
     4021 tcgcccggct cgccgagctc ctcggggacg ccgtgcgtct ccggcagcga ctcccgatgc
     4081 gctacctcat gatcgtcgga gcggcccttc ggggctggct agacgtctgc gagggatcga
     4141 ccgggggcat cgagaagatc gaaaggtcgg tcgcgcactc gcgcaccgat ggggagaccc
     4201 tccacctgac ctactgtctc ctcctgctcg ctcgggctcg tggtgtggtc ggcgagctcc
     4261 gcgaaggacg agccgccacg cgcgaggggc tctcgtggtc gcacagccgc gaccagcgct
     4321 acctcgaggc cgagctgtgg cgagtcgatg gtgagctggc gtatcgcggc ggagacggcg
     4381 aagccgctgc cgcttctctg cgcagtgcca tcgacgtcg
//
LOCUS       DBNGNK010000292         2123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_980955,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000292 DBNGNK010000000
VERSION     DBNGNK010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_980955"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="AC150V_08600"
     CDS             <1..1585
                     /locus_tag="AC150V_08600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3763017.1"
                     /translation="LCRIACDLIDCDRVSIFGLEDGYYRARFNHGNPPDIARFFERFR
                     VRADDPLVARAVEARSYVVVNEASGSRLMNQETARAARIRAIVVAPLLGADGSPLGFM
                     TAEYNDRLGRFSEQQSMLLLGAARIAAVLVLREQEQRRRGELERRVRSLERLEAIGRL
                     AGGVAHDFNNLLTVVLGNCEIVEAQVPEAAQPALAELRAAADRASALTQQLLAFGRRQ
                     ILEPRSVDVYALVESFDPLLRRLLPESIQIEIHGTREESFARVDPSEFEQVLMNLVLN
                     ARDAMPQGGRLTIETGSATLDEDYCREHPGALPGQYVVLAVSDTGVGIPSAVAEQIFE
                     PFFSTKPPSRGTGLGLATVYGVVQQSRGHVWVYSEPGKGTSFKVYLPRTDTSADETDA
                     ELPLVTSLGGTETVLVADDAEAVRRVARAALEAHGYTVIEASHPDEAAERARAHDGPI
                     HLLLTDVVMPGSSGRDLAERLLRERPELRVLYVSGYAENAIVHQGALDPGVAYLAKPF
                     TPTVLARRVREELDAQPSS"
     gene            1615..>2123
                     /locus_tag="AC150V_08605"
     CDS             1615..>2123
                     /locus_tag="AC150V_08605"
                     /inference="COORDINATES: protein motif:HMM:NF026257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR3763018.1"
                     /translation="MSDPSRPELELILCCSRLRVSPAIEERIRQLASPDIDWTRLVEL
                     ALANGTTPLLCQALGRAGPPGVPEEILEAFEVHADDNRRRNEHLAAVLLDVMGSLEEA
                     DISAIAFGGPVLAAFAYGDLSLRRADDPLALFVREADLQKSRDPLGRRGFRLAAEETP
                     TARRWIRDRG"
BASE COUNT          321 a          729 c          735 g          338 t
ORIGIN      
        1 actgtgtcgc atcgcctgcg atctgatcga ctgcgatcgc gtcagcatct tcgggctcga
       61 agacggctac taccgagcgc gcttcaacca cggcaacccg ccagacatcg cgcgcttttt
      121 cgagcggttc cgggtgcgcg ccgacgatcc gctcgtcgcg agagccgtcg aagcgcgcag
      181 ctacgtcgtg gtcaacgagg cttccggctc gcggttgatg aaccaggaaa ccgcgcgcgc
      241 cgccaggatc cgcgccatcg tcgtggcgcc gctgctgggc gccgacggct caccgctggg
      301 cttcatgacg gcagagtaca acgaccgtct ggggcgcttc tcggagcagc aatcgatgct
      361 cctgctgggc gctgcgcgca ttgcggccgt gctcgtgctt cgcgagcaag agcagaggcg
      421 gcgcggcgag ctcgagcggc gcgtccgcag cctggagcga ctggaggcga tcggccgcct
      481 ggcgggaggc gtcgcgcacg atttcaacaa cctgctcacc gtcgtcctgg gcaactgcga
      541 gatcgtcgag gcccaggtac ccgaggcggc ccaaccggcg ctcgctgagc tgcgggctgc
      601 cgccgaccgc gcctcggcgc tgacccagca gctgctggct ttcggccggc ggcagatcct
      661 cgagccccgc agcgtagacg tctacgcgct cgtcgagagt ttcgatcccc tgctgcggcg
      721 actgctgccc gagtcgatcc agatcgagat ccacgggacg cgagaggagt ccttcgcgcg
      781 agtcgatccg agcgagttcg agcaggtgtt gatgaatctg gtgctcaacg cgcgcgacgc
      841 gatgccgcag ggcggtcgcc tcaccatcga gaccggttcg gccactctcg acgaggacta
      901 ttgtcgagaa catccgggcg cattgccggg ccagtacgtc gtcctggccg tgagcgacac
      961 cggtgtgggg atcccgtcgg ccgttgccga gcagatcttc gagcccttct tctcgaccaa
     1021 gccgccgtcc cgcggcactg ggctcggact cgcgaccgtc tatggagtgg tgcagcagag
     1081 ccggggccac gtctgggtct acagcgagcc gggcaagggc acgagcttca aggtctatct
     1141 gccccggacc gacaccagcg ccgatgagac ggacgccgaa ctgccgctgg tgacgtcgct
     1201 cggagggacc gagacggtcc tggtggccga tgatgccgag gccgttcgac gtgtggcgag
     1261 agcggcgctc gaggcgcacg gctataccgt gatcgaggcc tctcatcccg acgaagccgc
     1321 cgaacgggct cgtgcccacg acggcccgat ccacctgctc ttgaccgacg tcgtgatgcc
     1381 cggatcgagc ggccgggatc tggccgagcg gctgctccgc gagcggccgg agcttcgcgt
     1441 cctgtacgtg tccggctacg ccgagaacgc gatcgtacac cagggtgcgt tggacccggg
     1501 cgtcgcctac ctcgccaagc ccttcacgcc cacggtgctg gcgcgccgcg ttcgcgagga
     1561 gctcgacgct cagccgtcct cctgacggca gcgcggttcg accccgcaac cgacatgtcc
     1621 gatccgagta ggcccgagct cgagttgatc ctgtgttgct cccggctccg cgtgagcccg
     1681 gcgatcgagg agcggatccg gcagctcgcg agtccggaca tcgactggac gcggctcgtc
     1741 gagctggccc tcgcgaacgg caccacaccg ctgctgtgcc aggcgctcgg tcgggcgggt
     1801 ccgccgggcg tgcccgagga gatcctcgaa gcgttcgagg tccacgcgga cgacaaccgc
     1861 cggcgcaacg agcatctcgc cgcggtactg ctcgatgtaa tggggtcgct ggaggaggcg
     1921 gacatctccg caattgcgtt cggggggccg gtactggcgg ccttcgcgta cggcgatctc
     1981 tcgctgcggc gcgccgacga cccgcttgcc ctcttcgttc gcgaagcgga cttgcagaag
     2041 tcccgcgacc cgctgggacg acggggtttt cgcctcgcag cggaagagac gcccaccgcg
     2101 cgtcgctgga tccgcgatcg ggg
//
LOCUS       DBNGNK010000293         6277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_766506,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000293 DBNGNK010000000
VERSION     DBNGNK010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_766506"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="AC150V_08610"
     CDS             complement(<1..651)
                     /locus_tag="AC150V_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3763019.1"
                     /translation="MPKLVDTTIRLLSQEPLVGRVPTGELLRIADILDGAGLACLEVS
                     GGGAFDAAVRRGVESPWERVRALDGRTKTPLGMALRGSFLVGSKPVSGDVVRRFVACA
                     AENGIDVFRLHDPLNDVSNLRDAGDAIVEAGKELHVGLVYSPGRTGEIDALVEQARRV
                     PELGASLVILNDPTDALLPHLTEELVQRLGETTGLPVGLYVQGAAGTGLLNAIVATR"
     gene            complement(713..1279)
                     /gene="efp"
                     /locus_tag="AC150V_08615"
     CDS             complement(713..1279)
                     /gene="efp"
                     /locus_tag="AC150V_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR3763020.1"
                     /translation="MADVVSTNQFKNGMHIEHKGSVWRIVEFQHVKPGKGGAFVRTKL
                     KNLDSGAVVDETFRAGEKFERVHTEVKTLQFLYDDGSDAHFMDQATFEQFALPVGEVE
                     EALEYTLPSATVQMLQVNGQPAGIQLPASVELSVSETEPGVKGDTVSNVTKAATLETG
                     AVVQVPLFVNIGDRIKVDPREARYISRA"
     gene            complement(1297..2334)
                     /locus_tag="AC150V_08620"
     CDS             complement(1297..2334)
                     /locus_tag="AC150V_08620"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3763021.1"
                     /translation="MRRIARLAGALEEPLLVTGSTNVRYLTGLASTNAALLVEPNRAT
                     LYSDFRYAEAAAEIEGIEFVELPRNLMAGLAEALAGRRIAFEAAHITYAGHQTLAAGG
                     VELVPSRGAVERLRAVKEPSEVDAIRRAAELSDQVFAELAEERFTGRTERELAWWIER
                     RFRERGAQGVSFPVVVASGAAGSRPHSVPGDRPIEAGTLVVVDAGCVVDDYCSDCTRT
                     FATGEISSELADAYALCLRAQLDGLSAVRPGVSGKDVDAASRTAIEAAGLGDRYRHGL
                     GHGVGLAVHEEPALRPESADVLESGNVVTVEPGIYFAGQAGVRIEDLVLVTGDGSERL
                     TRFPKELNTVE"
     gene            complement(2331..2762)
                     /locus_tag="AC150V_08625"
     CDS             complement(2331..2762)
                     /locus_tag="AC150V_08625"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR3763022.1"
                     /translation="MRIDVLNGVNLDLLGRRDPEHYGEASLQDLETRVYAWARELSVQ
                     VRCRQTNHEGEYVEWLHQALTGADALIVNPGAWTHYSWAIRDALEAFEGTVVEVHLSN
                     VDEREEWRRFSVLEGLASKRIVGIGIEGYRDALSYLVEGGP"
     gene            complement(2772..4175)
                     /locus_tag="AC150V_08630"
     CDS             complement(2772..4175)
                     /locus_tag="AC150V_08630"
                     /inference="COORDINATES: protein motif:HMM:NF025065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HIR3763023.1"
                     /translation="MGAGKTSLGRELARRLDREFIDLDELIEEREGATISELFAGRGE
                     SGFREAETRHAVDALSAPSLRVIALGGGALISEATREALRERAFTTWVEVDPEVAWKR
                     IEGTDRPLARDRASFFQLYESRRTLYEAAADGRARDSDGAILAAAGVHVQTGALAELA
                     SLLPGDWPVELVVDATVNGIHGVDAQLALGARDIAVHEVPAGEAAKTPAVLERLWRAL
                     RVGRDGAIVALGGGATTDVAGFAAASYLRGISWIPVPTTLVGQVDAAIGGKTAVDLPE
                     GKNLVGAFHWPARVIIDPAVLETLPERELRNGRAEVVKTGLLVGRPVWERPLLEQVRL
                     CAAFKSALCLCDPHDRGPRTQLNLGHTFAHALEASVGYELPHGEAVALGLLAAVRLSV
                     RHLGLDPAVAHEVEDVVGATAVRVDPDAAWAALTRDKKAVGGSPRLVLLEAPGRPVWG
                     VELPADDVRTALAELIA"
     gene            complement(4211..5302)
                     /locus_tag="AC150V_08635"
     CDS             complement(4211..5302)
                     /locus_tag="AC150V_08635"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR3763024.1"
                     /translation="MVLSLLTAGESHGPALVAILNGVPAGLSLDQDAINGDLRRRQQG
                     YGRSPRQKLESDEVEVLAGLRHGKTLGTPLALVVRNRDHKNWTWGMSPWPPEGDASGK
                     GTKPVTLPRPGHADLAGVLKYAHDDVRNALERASARHTAVHVAAGGVAKALLKEIGVT
                     VVGRAVEVGGASGDDAMRAATDAARKDRDTLGGVVEVTAAGVPPGLGTYAEKADRLDA
                     RLVGALAGIQAVKGVEVGEGFALARKRGSEAHDEIDPGPTRRTNRAGGIEAGISNGED
                     VVVRAAMKPLPTLMRPLDSVDLDTGEPAQALVERSDVQAVEALAVVAEAAVSWELARA
                     AKEKFGGDALVDFVAAYASYMERIGWDTR"
     gene            complement(5345..6091)
                     /locus_tag="AC150V_08640"
     CDS             complement(5345..6091)
                     /locus_tag="AC150V_08640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR3763025.1"
                     /translation="MIEIAGTTTLVGILGWPTSHSLSPRMQNAGFAALGLDWAYVPLP
                     TPPDRLHEAVAGLGAAGFAGANVTIPHKEHVMASCDELDDVAERAGSVNTLVFRDGQV
                     HGSSTDGAAVVDQVDAEGRTALVLGTGGAAKAIAAALRAAGADVVVTGRRDRDWPPSS
                     DGMELLVNATPVRDELIVEPQEWMQVVDLAYDRAGTPTALVAAAREAGCEVVVDGLDV
                     LLLQGAASFERWTGAPAPLAAMRSALRNGT"
BASE COUNT          951 a         2271 c         2108 g          947 t
ORIGIN      
        1 acgcgtcgcg acgatcgcgt tgaggagccc ggttcctgcg gcgccctgca cgtacagacc
       61 gacgggcagt cctgtcgtct cgcccagccg ttggacgagc tcctccgtca gatgcggcag
      121 gagggcgtcg gtcgggtcgt tcaggataac gagcgacgcc cccagctcag gcacgcgccg
      181 ggcctgctcg acaagggcgt cgatctcgcc ggtccggcct gggctgtaga cgagccccac
      241 gtggagctcc ttccctgcct ccacgatcgc gtctccagca tcgcgcaggt tcgagacgtc
      301 gttcaggggg tcgtggaggc ggaagacgtc gatgccgttc tctgccgcac aggccacgaa
      361 ccgacgcacg acgtcgccgg agaccggctt cgacccgacc agaaacgagc cgcgcagagc
      421 catcccgagc ggcgtcttcg tccgtccgtc cagcgcgcga acacgttccc acggactctc
      481 cacccctctg cgcacggcgg cgtcgaaggc accgccgccc gacacctcca ggcacgcaag
      541 acccgctccg tcgagaatgt ctgcgatgcg gagcagctcg cccgtcggca cccgcccgac
      601 gagcggctcc tgcgagagca gccggatcgt ggtatcgacc agcttcggca cgggcagacc
      661 ttagcgagtg ctgcgatgag agcaacacgg cgccgcgcgc gaagacggcg cgtcaggccc
      721 gggagatgta gcgggcctcg cgggggtcga ccttgatccg gtcgccgatg ttgacgaaca
      781 gggggacttg gacgactgcc ccggtctcga gcgtcgccgc cttggtcacg ttggagacgg
      841 tgtcgccctt cacaccgggt tcggtctcgc tcaccgacag ctcgacggag gcggggagct
      901 ggatccctgc gggctgcccg ttgacctgca gcatctggac tgtcgcggac ggcagggtgt
      961 actccagcgc ctcctcgacc tcgccaaccg gcagcgcgaa ctgctcgaac gtcgcctgat
     1021 ccatgaagtg ggcgtccgag ccgtcgtcgt agaggaactg cagggtcttg acctcggtgt
     1081 gcacgcgctc gaacttctcg ccggctcgaa acgtctcgtc gacgacggct cccgagtcga
     1141 gattcttgag cttcgtccgg acgaacgcgc cgcccttccc cggcttcacg tgctggaact
     1201 ccacgatccg ccacaccgag cccttgtgct cgatgtgcat tccgttcttg aactggttgg
     1261 tcgagacgac gtcggccata cggccgcgca gtgtactcac tcgaccgtgt tcagctcctt
     1321 ggggaaacgg gtcaggcgct cgctgccgtc gccggtcacg agcacgagat cctcgatacg
     1381 cacaccggcc tgcccggcga agtagatacc cggctcgacc gtgacgacgt tcccgctctc
     1441 gagcacgtcc gcggactcgg ggcggagggc cggctcttcg tggactgcaa ggccgacgcc
     1501 gtggccgagc ccgtgccggt accgatcgcc cagaccagcc gcctcgatag ccgtcctgga
     1561 ggccgcgtcg acatccttgc cgctgacgcc cgggcgcacg gcggacaggc cgtcgagttg
     1621 agcacgcaga cagagcgcgt aggcgtccgc gagttcagat gagatctctc ccgtcgcgaa
     1681 cgtacgcgta cagtccgagc agtagtcgtc gaccacgcag cctgcgtcga cgacgaccaa
     1741 cgtgcccgcc tcgatgggtc ggtcgccggg gacggagtgg ggacgcgagc ccgcagcacc
     1801 cgaagcgacg acgaccggaa acgagacccc ttgcgcgccc cgctcgcgaa agcgccgttc
     1861 gatccaccat gcgagctcac gctcggttcg tccggtgaag cgctcctctg ccagctccgc
     1921 aaagacctga tccgagagct cggccgcccg ccggattgcg tccacctcgc tcggctcctt
     1981 caccgcgcga agacgctcca cggcgccgcg gctcgggacg agctcgacgc cgcccgccgc
     2041 caacgtctgg tggcccgcgt acgtgatgtg cgcggcctcg aacgcgatgc gtcgccccgc
     2101 gagtgcttct gccagcccgg ccatgagatt gcgcggaagc tcgacgaact cgatgccctc
     2161 gatctccgcg gccgcctcgg catagcggaa gtcggagtac agggtcgccc tgttcggctc
     2221 gacgaggagc gcggcgttcg tgctcgcgag cccggtcagg tagcggacgt tagtcgagcc
     2281 ggtgacgaga agcggttctt cgagcgcgcc ggcgagtcgc gcgatgcgcc tcacgggcct
     2341 ccttcgacga gataggaaag cgcgtcgcga tagccctcga tgccgatacc cacgattcgc
     2401 ttcgaagcga gcccctcgag caccgaaaag cgccgccact cctcacgctc gtcgacgttc
     2461 gagaggtgca cctcgacgac ggtgccctcg aacgcctcta gcgcatcccg gatcgcccag
     2521 gagtaatgcg tccatgcgcc tgggttgacg atcagcgcgt cggcgcccgt cagggcttgg
     2581 tggagccact cgacgtactc gccctcgtgg ttcgtctggc gacagcgaac ctggacggac
     2641 agctcacgcg cccaggcgta gacgcgcgtc tcgagatcct ggagcgaggc ctcgccgtag
     2701 tgctccggat cgcgtcgccc gagcagatcg agattgacgc cgttgagcac gtcgatccgc
     2761 atcgaccgac cttacgcgat cagttcggcg agcgcggtgc ggacgtcgtc ggccggcaac
     2821 tcgacgcccc acacgggtcg gccgggcgcc tcgagcagaa cgagacgagg cgagccgccg
     2881 accgccttct tgtcacgcgt gagcgcagcc caagccgcat ccgggtcgac acggacggcc
     2941 gtcgcgccga caacgtcctc gacttcgtgc gcgacggcgg gatcgaggcc gaggtggcgc
     3001 acggagagcc gaaccgccgc gagcagcccg agagcgacgg cttcgccgtg cggcagctcg
     3061 tagccgaccg aggcctccag agcgtgggcg aatgtgtgac cgaggttgag ctgcgtgcgc
     3121 ggcccacggt cgtgtggatc gcagaggcac agcgccgact tgaacgcggc gcagagccgt
     3181 acctgctcca acagcggtcg ctcccagacc ggccgcccca ccagtagccc ggtcttgacg
     3241 acctccgcgc ggccgttccg gagctcccgc tcgggcagcg tctcgagcac cgcggggtcg
     3301 atgatcacgc gcgccggcca atggaacgcg ccgacgaggt tcttgccctc gggcaggtcg
     3361 accgccgtct tcccgccgat cgccgcgtcg acctgaccca cgagcgtggt cggcaccggg
     3421 atccacgaaa tcccgcgcag gtagctggcg gcggcgaaac cggcgacgtc ggtcgtcgct
     3481 cctccgccga gcgcgacgat tgctccgtcg cggccgacgc gaagcgcccg ccagagacgt
     3541 tccagcaccg cgggggtctt ggccgcctcg cctgcgggaa cctcgtgcac ggcaatgtcg
     3601 cgggctccca gcgccagctg cgcgtcgaca ccgtggatgc cgttcaccgt cgcgtccacc
     3661 acgagctcga cgggccaatc gccgggaaga agcgacgcga gctcggcgag cgcaccggtc
     3721 tggacgtgaa cgccggccgc cgcgaggatc gcgccgtcgc tgtcgcgcgc ccggccgtcg
     3781 gcggccgcct cgtagagcgt gcggcgggac tcgtacagct ggaagaacga agcgcgatcg
     3841 cgggcgaggg gccggtcggt cccctctatc cgcttccagg cgacctccgg gtcgacctcg
     3901 acccaagtcg tgaacgcccg ctcccgcagc gcttcccgcg ttgcctcgga gatcagggcg
     3961 ccgccgccga gcgcgatcac gcggagcgaa ggcgcagaga gtgcatcgac cgcatggcgg
     4021 gtctcggcct ctcggaatcc cgactctccc ctgcctgcga agagctcgga aatggtcgcg
     4081 ccttctcgct cctcgatcaa ctcgtcgagg tcgatgaact cacgatcgag ccgacgggcg
     4141 agctcacgac ccagcgacgt cttgccggcg cccatgaacc cggccagggc caggtgccgg
     4201 tcgagcgcgt tcagcgggtg tcccagccga tcctctccat gtacgaggcg taagccgcga
     4261 cgaagtcgac gagggcatcg ccgccgaact tctccttcgc ggcccgtgcc agctcccagg
     4321 agaccgccgc ctcggcgacg acggcaagcg cctccacggc ttggacatcg ctccgctcga
     4381 cgagcgcctg ggcgggctcg ccggtatcga ggtccacgga gtcgagcggt cgcatcagcg
     4441 tcggaagcgg cttcatcgcg gcgcggacga ccacgtcctc gccgttcgag atgccggcct
     4501 cgatcccccc ggcgcggttc gttcgcctcg tcgggcccgg atcgatctcg tcgtgagcct
     4561 cggatcctcg tttgcgagcg agggcgaacc cctcccccac ctcgactcct ttcaccgcct
     4621 ggatgcccgc gagcgcgccg acgagccgcg cgtcgaggcg atcggccttc tccgcgtacg
     4681 tgccgaggcc gggcggcact cccgcggcgg tcacctcgac gacgccgccg agggtgtcgc
     4741 ggtccttgcg ggcagcgtcc gtcgcggctc tcatggcgtc gtcaccgctc gccccgccga
     4801 cctcgacggc gcgcccgacg actgtgacgc cgatttcctt gagcagcgct ttggcgacgc
     4861 cgcccgcggc gacatgtacc gccgtgtgcc gggcactggc ccgctcgagc gcgttgcgaa
     4921 cgtcgtcatg tgcgtacttg agcacaccgg caaggtcggc atggccgggt cgcggcagtg
     4981 tcaccggctt tgtccctttg ccacttgcgt ctccctcggg cggccatggg ctcatgcccc
     5041 acgtccagtt cttgtggtcg cggttgcgga cgacgagcgc cagcggagtg ccgagcgtct
     5101 tcccgtgcct gagtcccgcc agcacctcga cctcgtcgct ctccagcttc tgccgcgggc
     5161 tgcggccgta cccctgctgg cgtcggcgta gatcgccgtt gatcgcgtcc tggtcgaggc
     5221 tcagcccggc gggtacgccg ttgaggatcg cgacgagtgc cggcccgtgg gactcgccgg
     5281 cggtgaggag gctgagaacc acgctcgaag cgtagatggg agggcgggcg gcaaggaacg
     5341 ggtgttacgt gccgttccgg agcgcggagc gcatcgctgc aagcggagcc ggcgcgcccg
     5401 tccagcgctc gaaggacgct gcgccctgca agagcagtac atcgagcccg tccaccacca
     5461 cctcgcaccc agcttcgcgg gcggccgcaa cgagcgctgt cggggtgccg gcccggtcgt
     5521 acgcgagatc gaccacctgc atccactcct gcggctcgac gatcagctcg tcgcggactg
     5581 gcgtcgcgtt gacgagaagc tccatgccgt cgctcgacgg cggccagtcc ctgtcacggc
     5641 gtcccgtgac gacgacgtcc gcgccggccg cgcgaagggc cgccgcgatc gccttcgcag
     5701 cgccacccgt gccgaggacg agcgcggttc tcccctcggc gtcgacctga tcgacgaccg
     5761 cggccccgtc cgtgctcgat ccgtgcacct gcccgtcgcg gaagaccaac gtgttcaccg
     5821 agcccgcccg ttctgccacg tcgtccagct cgtcgcacga cgccatgacg tgctccttgt
     5881 ggggaatcgt cacgttcgct ccggcgaagc ccgccgcgcc gagcccggcg acggcctcgt
     5941 ggagccggtc cggaggggtc ggcagcggca cgtacgccca gtcgagcccc agcgccgcga
     6001 agccggcgtt ctgcatcctt ggcgagaggg aatgggacgt cggccagccg aggatgccga
     6061 ccagcgtcgt cgtccctgcg atctcgatca tgtgggcagt atcgaccctc gccgcgcccg
     6121 catgtccaca gcggccatgt gggacctcag aggtgggaca cgtctttcgg gggaatacgc
     6181 cgtcacggct gtgggtatcg gccgcctagc tgtgggtgag tgaccacgcg cctgtggaag
     6241 aaagctcaat cgcaggcgta cccgaactcg cacacca
//
LOCUS       DBNGNK010000294         5680 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1627959,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000294 DBNGNK010000000
VERSION     DBNGNK010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1627959"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..556
                     /locus_tag="AC150V_08645"
     CDS             92..556
                     /locus_tag="AC150V_08645"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HIR3763026.1"
                     /translation="MGQRHPRSTLCERTRAWASLSLDSELSEFERALFQSHLERCAEC
                     ASFSADIASVTSELRAAPLEALPHPITLPARRRRFGGSALRVGAAAAVVVGALGLAGS
                     LSLPSDSTQEAPEVDVRTSEQLDDRLIREPRLDSLKPTPPADPGRKVLGLPL"
     gene            803..2236
                     /gene="atpD"
                     /locus_tag="AC150V_08650"
     CDS             803..2236
                     /gene="atpD"
                     /locus_tag="AC150V_08650"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR3763027.1"
                     /translation="MPDQSNGKNIGKIVEIKGVVLDAVFPDELPEILNALEIQVPKED
                     GSSTTLVAEVQQHLGDDRVRAVAMDSTDGLARGSDAVDTGVAISVPVGDQTLGRLWNV
                     IGDPIDEREEPLQGAERWPIHRDPPTFRDLSPTVEIFETGLKVVDLIAPYIKGGKVGL
                     FGGAGVGKTVLIMELIHNVANAHAGVSVFAGVGERTREGNDLWLEMSESGVIDKVALV
                     YGQMNEPPGARLRVALSGLTMAEYFREEGQDVLLFIDNIFRFVQAGSEVSALLGRMPS
                     AVGYQPTLATEMGQLQERITSTRTGSVTSVQAIYVPADDLTDPAPANTFAHLDATTVL
                     SRAIVEKGIYPAVDPLDSTSRALQPGIVADDHYATATAVQQILQRYKDLQDIIAILGM
                     DELTDEDKLVVARARKIERFLSQPNFVAEQFTGTPGKYVKLEDTIRGFRMIIDGECDE
                     LPETAFYMVGPIEEAFEKAREAEQVAA"
     gene            2244..2675
                     /gene="atpC"
                     /locus_tag="AC150V_08655"
     CDS             2244..2675
                     /gene="atpC"
                     /locus_tag="AC150V_08655"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR3763028.1"
                     /translation="MADVHPKFDVALVTPEGAVFDGEAEMLIVPGAAGEIGILARHAP
                     LVATLKAGSTRVHLNENEILEFATGPGFFKVELDRALALVDDAVNVKEIDDERARAQL
                     EAAKSELERVDAGDSAADRWQLEQRLKHAENQLSVSGRTTG"
     gene            2763..>5680
                     /gene="metH"
                     /locus_tag="AC150V_08660"
     CDS             2763..>5680
                     /gene="metH"
                     /locus_tag="AC150V_08660"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR3763029.1"
                     /translation="MTVAERLRDLLAERILVLDGSWGVLMHRENLSEEEYRGERFADH
                     PRDVRGNPDLLNLTRPDLVLDTHRTYLAAGADLTTTNTFTATSIGQADYGLETFAYEL
                     SREGARLARQACDEWSEQTPAQPRFVAGSVGPLNVSLSVSPRVDDPAFRAVTFDRVRE
                     SYAEQIRGLVDGGADLLLIETIFDTLNAKAAIAAALDVAPETPRWLSFTAIDRSGRNL
                     SGQTVEAFWISLEHAEPLIVGVNCSLGAHEMRPFLADLARVATTYVACHPNAGMPNAF
                     GEHDETPEITSALLREFAEDGLVNLVGGCCGTTPEHTGKIARAMDGLRPREIPTPIRR
                     PRFAGLEPFVVDDDTAFIVVGERSNVTGSARFRELVEGDDWQGAVDVALGQVRGGANL
                     LDVNMDADLLDGVQAMTTFLNLLASEPEAARLPVVVDSSRWEVIRAGLTCLQGKGIVN
                     SVSLKEGEEDFLAKAREVRRFGAGVVVMAFDESGQAETADRKVEILGRAYDLLIQEAG
                     FAPEDIVLDPNVLAVATGIEEHDLFAREFIDAVPRLKKRCPGALVSGGISNLSFSFRG
                     NAIVREAMHSVFLYHAISAGLDMGIVNAGQLAVYEDIPPDLLELVEDVVLARRPDATD
                     RLVAAAEGVRGEGVRREVDLTWREATVDARLSHALVHGVVDFVEEDTEEARRLSDRPL
                     DVIEGPLMDGMKIVGDLFGEGKMFLPQVVKSARVMKRAVAYLEPYMEAEKTTAERVGR
                     LVLATVKGDVHDIGKNIVGVVLGCNGYEVIDLGVMTPGDVILDTALENDCDIVGLSGL
                     ITPSLDEMVVVAKEMERRGLTLPLLVGGATTSRQHTAVRIAPEYSAPTVHVLDASRVA
                     GVVGALLDPARREQLAQENHAEQERLRGLHQDRARRPLLAYEEAVANRPAIDFRADEL
                     PAPAFTGVRRAEPSVAELRDYIDWTFFFHAWELRGRFPKILERPEARELYDDACRLLD
                     "
BASE COUNT          933 a         1937 c         1924 g          886 t
ORIGIN      
        1 cgacagccgc tttagcctct actgacgaaa tcttcaccgg tcgataatcg atcagaacta
       61 acctcttccg cggaggcgcg tcataaacga catggggcaa aggcacccga gatctactct
      121 gtgcgagcgc acgcgggcgt gggcttcgct ttccctcgac agcgagctgt ccgagttcga
      181 gcgcgcgctc tttcaatcgc acctcgagcg ctgcgccgaa tgcgcgtcct tcagcgccga
      241 catcgcttcg gtgacgagcg agcttcgcgc ggccccgctc gaggccttgc cccatccgat
      301 cacgcttccc gcgcggcgcc gtcgcttcgg tgggagcgct ttgcgcgtgg gcgccgccgc
      361 cgccgtcgtg gtcggggcgc tcggtctcgc agggtcactc tccctgccga gcgactcgac
      421 ccaagaagcg cccgaggtcg acgttcgcac ctcggagcag ctcgacgatc ggctcatccg
      481 tgagccgagg cttgactcgc tgaagccgac tcccccggcc gatcccggcc ggaaggtgct
      541 cgggctcccg ctgtagcggg tagcactccc agccgtcgag aggccccgag gctctacgct
      601 gtgggcatgc tcgcgcagcc ctcgacgatc acgtcgcact gctgacacct cgaccacgcc
      661 gccaggctgg cggcggcgct cgaacgagtc gaccccggcg gtacgagcgg tcgcgggcgg
      721 aggcgctcgg gtaggatacg ggcgcttttg cggcgcctgc gcgctgctcc tccatcagtc
      781 agcgaagcac tccaggagac ccatgcctga ccagtcgaac ggcaagaaca tcggcaagat
      841 cgtggagatc aagggcgttg tcctcgatgc cgtcttcccc gacgagctgc ccgagatcct
      901 caacgcgctc gagatccagg tcccgaagga agatggctcg tcgacgactc tcgtcgccga
      961 agtgcagcag catctcggcg atgaccgtgt ccgcgccgtg gcgatggact cgaccgacgg
     1021 tctcgcgcgc gggtcggacg cggtcgacac cggtgtggcg atctccgtcc cagtcggcga
     1081 ccagaccctc gggaggctct ggaacgtcat cggtgacccc atcgacgagc gcgaagagcc
     1141 tctacagggt gccgagcgct ggccgatcca ccgcgatcca ccgacctttc gcgacctctc
     1201 gcccaccgtc gagatcttcg agacggggct gaaggtcgtc gacctgatag ccccctacat
     1261 caagggcggc aaggtcggtc tcttcggtgg cgcgggtgtg ggcaagacgg tgctgatcat
     1321 ggagctgatc cacaacgtcg cgaacgcgca cgccggcgtc tcggtcttcg ccggggtcgg
     1381 cgagcgtacg cgcgagggca acgacctgtg gctcgagatg agcgagtccg gcgtgatcga
     1441 caaggtcgca ctcgtctacg ggcagatgaa cgagccgccg ggcgcccgcc tgcgggttgc
     1501 gctctcgggg ctcaccatgg ccgagtactt ccgtgaggaa gggcaggacg tgctgctctt
     1561 catcgacaac atcttccgct tcgtccaggc gggctcggag gtgtcggctc tgctgggccg
     1621 gatgccgagt gccgtcggct accagcccac gctggccacc gagatgggcc agctacagga
     1681 gcggatcacg tccacgcgca ctgggtcggt cacgtcagtg caggcgatct acgtccccgc
     1741 cgacgacctc accgatcccg cgcccgccaa caccttcgcg cacctcgacg ccacgaccgt
     1801 cctctcacgc gcgattgtcg agaagggcat ctatccggcc gtcgacccgc tcgactcgac
     1861 ctcccgtgcc ctccagccgg gcatcgtggc ggacgaccac tacgcgacgg cgacggcggt
     1921 gcagcagatc ctgcagcgct acaaggatct gcaggacatc atcgcgatcc tcggcatgga
     1981 cgagctgacg gacgaggaca agctcgtcgt ggcgcgcgcc cgcaagatcg agcgcttcct
     2041 ctcacagccg aacttcgtcg ccgagcagtt cacgggaacg ccgggcaagt acgtgaagct
     2101 ggaggacacg atccgcggct tcaggatgat catcgacggc gaatgcgacg agctgccaga
     2161 gacggccttc tacatggtcg gtccgatcga ggaggccttc gagaaagcgc gcgaggccga
     2221 gcaggtcgcc gcctagctca gacatggccg acgtccaccc gaagttcgac gtcgcgctcg
     2281 tgacgccgga aggcgcggtc ttcgacggcg aggccgagat gctgatcgtg cccggtgccg
     2341 ccggcgagat cggcatcctc gcccggcacg ccccgctcgt cgccacgctc aaagcgggat
     2401 cgacgcgggt gcacctcaac gagaacgaga tcctggagtt cgcgaccggg ccgggcttct
     2461 tcaaggtcga gctcgaccgc gcgctcgcgc tcgtcgacga tgccgtcaac gtgaaggaga
     2521 tcgacgacga acgggctcga gcacagctgg aggcggccaa gtccgagctc gagcgggtcg
     2581 acgccgggga ttcggcggcc gaccgctggc agctggagca gcgcctcaag cacgcagaga
     2641 accagctctc ggtctccggt cgcaccaccg ggtagccgcc ccaccgcggc gacatccacc
     2701 tgcgggcagc ttgccggccg gcaccactcg tgccgggaag ccccacgtct acgctttccc
     2761 gaatgaccgt tgccgaacgt ctacgcgatc ttctcgcaga gcgcattctc gttctcgacg
     2821 gctcgtgggg agtcctcatg caccgcgaga atctctcgga ggaggagtat cggggcgagc
     2881 gattcgccga tcacccgcga gacgtacgcg gcaacccgga tctgctcaac ctgacgcggc
     2941 cggacctcgt cctcgacacg caccgtacct accttgccgc cggtgcagac ctcaccacga
     3001 ccaacacgtt cacggcgacg tcgatcggtc aggcggacta cggtctcgag acctttgcgt
     3061 acgagctgag ccgcgaagga gcccgcctcg cgcggcaggc ctgcgacgag tggagcgagc
     3121 aaacgccggc gcagccacgc ttcgtcgcgg gctcggtcgg cccgctcaac gtctcgctct
     3181 ccgtctcgcc gcgcgtggac gatcccgcct tccgcgcggt caccttcgac cgggtgcgcg
     3241 aaagctatgc ggagcagata cgcggactgg tcgacggcgg agccgacctc ctcctgatcg
     3301 agacgatctt cgacacgctc aacgcgaagg ccgcgatcgc ggccgcgctc gacgttgccc
     3361 ccgagacgcc gcgctggctg tccttcaccg cgatcgacag gagcggccgc aacctgtccg
     3421 gccagacggt ggaggcgttc tggatctcgc tggagcacgc ggagccgctg atcgtcggcg
     3481 tgaactgctc gctcggcgcg cacgagatgc gccccttcct cgccgatctc gcgcgggtgg
     3541 ccacaacgta cgtcgcatgc catccgaacg ccggtatgcc gaacgccttc ggcgagcacg
     3601 acgagacccc ggagatcacg agcgcgcttc tgcgtgagtt cgccgaggac ggcctcgtca
     3661 acctcgtcgg gggctgctgc ggcacgacac ccgagcacac aggaaaaatc gcaagagcaa
     3721 tggacggtct gcggccgagg gagatcccga cgccgatccg caggccgcga ttcgctggcc
     3781 tcgagccgtt cgtcgtcgac gacgacaccg ctttcatcgt tgtgggcgag cggagcaacg
     3841 tgaccgggtc ggcccgcttc cgtgagctcg tggaggggga cgactggcag ggggccgtcg
     3901 acgtcgcgct cgggcaggta cggggcggag ccaatctcct cgacgtgaac atggacgccg
     3961 acctgctcga cggcgtgcag gcgatgacga ccttcctgaa cctgctcgcg tccgagccgg
     4021 aggcggcgcg tctgccggtg gtcgtcgaca gctcccgctg ggaggtcatc cgcgccgggc
     4081 tcacctgcct ccaggggaag ggcatcgtca actcggtgag cctcaaggag ggggaggagg
     4141 acttcctcgc caaggctcgc gaggtgcggc gcttcggggc gggcgtcgtc gtcatggcct
     4201 tcgacgagtc cggacaggcg gagacggcgg accgcaaggt cgagatcctc gggcgcgcct
     4261 acgacttgct catccaggag gccggcttcg cgccggagga catcgtcctc gacccgaacg
     4321 tcctcgcggt cgcgacgggg atcgaggagc acgatctctt cgccagggag ttcatcgacg
     4381 ccgtgccgcg cctgaagaag cgttgcccgg gcgcgctcgt ctccggtggc atctcgaacc
     4441 tctcgttctc gtttcgcggg aacgcaatcg tccgcgaggc gatgcactcg gtcttcctct
     4501 accacgcgat ctcggccgga ctggacatgg ggatcgtcaa cgccggtcag ctcgccgtgt
     4561 acgaggacat cccgccagat ctgctcgagc tcgtagagga cgtcgtgctg gcccggaggc
     4621 ccgacgcgac cgatcggctc gttgcggccg ctgagggggt tcgcggtgaa ggggtgcgtc
     4681 gcgaggtcga cctcacgtgg cgggaggcga ccgtcgacgc acgcctctcg cacgccctgg
     4741 tgcacggcgt cgtcgatttc gtggaggagg acaccgagga ggcccgtcgc ctctccgacc
     4801 gcccactgga cgtgatcgag gggccgttga tggacgggat gaagatcgtc ggtgatctct
     4861 tcggggaagg aaagatgttc ctgccgcagg tcgtcaagag cgcgcgcgtg atgaagcgcg
     4921 cggtcgcata cctcgagccg tacatggagg cggagaagac cacagccgag cgggtcgggc
     4981 gcctggtgct cgcgacggtg aagggcgacg tgcacgacat cgggaagaac atcgtcggtg
     5041 tcgtgctcgg ctgcaacggg tacgaggtga tcgacctcgg cgtgatgacg ccgggagacg
     5101 tcatcctcga taccgcgctc gagaacgact gcgatatcgt cggcctctcc ggcctgatca
     5161 cgccgtctct cgacgaaatg gtcgtcgtgg ccaaggagat ggagcgccgc ggcctcacac
     5221 tgccgctcct ggtcggtggt gcgacgacgt cgcgccagca cacggcggtg aggatcgcgc
     5281 ccgagtactc ggccccgacg gtgcacgtgc tcgatgcctc tcgagtcgcg ggagtggtcg
     5341 gggctcttct cgatccggcg cggcgggagc agttggcgca ggagaaccac gccgagcagg
     5401 agcggctgcg cggtctgcac caggatcgtg cgcggcgccc gctgctcgca tacgaggagg
     5461 ccgtcgccaa ccgtccagcg atcgacttcc gggccgacga gctgccggcg ccggcgttca
     5521 ccggcgttcg ccgggccgag ccgtcggtgg ccgagctacg cgactacatc gactggacgt
     5581 tcttcttcca cgcctgggag ctgcggggtc gcttccccaa gatcctcgaa cggccggagg
     5641 cgcgcgagct ctacgacgac gcctgccggc tgctcgacga
//
LOCUS       DBNGNK010000295         5601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1697374,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000295 DBNGNK010000000
VERSION     DBNGNK010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1697374"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="AC150V_08665"
     CDS             <1..898
                     /locus_tag="AC150V_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR3763030.1"
                     /translation="EAGAGAVRAAHQGRGGVVVVRALILLTAITVLGIGSAPAASSQV
                     AGSVRYLEAVQQSDGGFAEGGQPADAALTAWGALALAAGGGSDAARERALAFLRTHER
                     DETGGAALALEVLARAALGDRPDGLARRLRDHRPGTLVNETIWTILALRATEEQVPRS
                     YVKAVLASQHAAGGWSWARRGSPDSNDTAAAIQALRAVGVTGAPIKRALAALRGFQNR
                     DGGFGLVKGRASDVQSTAWAIQAFLAAGAKPGKPAFRYLSRLRRPDGSYRYSTAYGTT
                     PVWVTAQVVPALAGRPFPLAGR"
     gene            947..3109
                     /locus_tag="AC150V_08670"
     CDS             947..3109
                     /locus_tag="AC150V_08670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR3763031.1"
                     /translation="MSAALPTEYLADLNEPQREAVLAVEGPVLVVAGAGSGKTRVLTH
                     RVAHLIRACGVKPNEILAITFTNKAAGEMRERLERMLGHTARAIWILTFHSACGRILR
                     REAARLGYRSNFTIYDQADQVRVVKACLEELGRDPKRFTPRGIHVQISNAKNRLVGPD
                     EYMARVSSFYDQTVAEVYELYQRKLFESNAVDFDDMLMLTVDLLERFPEARGHWQRAF
                     RYVLVDEYQDTNHAQYRLLRLLAAEHGNVFAVGDPDQSIYAFRGADIRNILEFERDFE
                     GTRVIALEQNYRSTNAILSAANGVIEHNRGRKPKRLYSDLGEGEPVQVIEVEDEHAEA
                     RFVAAEIARLVGDGWSASELAVFYRTNAQSRVLEDVLVRQDVPYQVIGGPRFYERAEI
                     KDAVAYLTVLDNTQDAVAVMRIANRPRRGIGDTSIQRLVSHAETLGISLWEALADPGA
                     AGLATAPAKAVRGFHTLMQSLQSAAQELEIDELIEAVLERSGTIDALEAERTIEARGR
                     IENLQELVGVAREYRETRDEPTLSGFLQEISLVSDQDALRDDDTGVVTLMTIHNAKGL
                     EFRGVFVIGMEEGIFPHSRSIEDNEVEEERRLCYVGMTRAMERLTLTHAMARSLFGRR
                     DYNLASRFLDELPASVERERLRPSSWSGYAASPRQVAPQDPVDLPALQTGDSVEHGSL
                     GEGVVTRMEPGGIVTVRFAQDGTERRLMLEYAPLRKVG"
     gene            3109..4329
                     /locus_tag="AC150V_08675"
     CDS             3109..4329
                     /locus_tag="AC150V_08675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3763032.1"
                     /translation="MAFRVRRVRDLDEYRAALGVIGHYFGWNPSEEDAERFSRHLPVE
                     RMHAAWEGSEIVAGTGAIPFELTVPGGPAPCAGVTVVAVRPTHRRRGLMRRLMLAQLA
                     DIRERAEPIAALWASEETIYGRYGYGLAELLQIEMRLPREYTRLRGESDPVGTVRLVD
                     HDEALRTFPRVYERVRRNTVGFLSRSREWWDLRRLDDAPERRRGMGPLNRALLEIDGR
                     PAGYALYRVKSEFEGAVNMGHVQVIEALGVSAEATREIWRFALGVDWLGEVRTALLPL
                     DHALLQLVTHPNRMRWQIADGIWLRPIDVGAALAARSYSGDGRVTLEIAADPQFPDNV
                     QRYTIEGGDVRRTRRRPDVRLDVQALGSAYLGGFTFAALAAGGRVQEVTRGGLARADA
                     LFRTDRLPWTPELF"
     gene            4367..4816
                     /locus_tag="AC150V_08680"
     CDS             4367..4816
                     /locus_tag="AC150V_08680"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3763033.1"
                     /translation="MARFREVIGVSLPLEASFAYVADFTTAAEWDPGILESRAVDEGE
                     PRVGSRYDVVANFRGRSIPFRYEILQLEPGRRIVIRGEGDKATSDDTIEFEPAGPGTR
                     ISYEAELKMKGIWRAAEPFLGGTFATMGKKALHGLKEQLDRRGAEAA"
     gene            4813..5214
                     /locus_tag="AC150V_08685"
     CDS             4813..5214
                     /locus_tag="AC150V_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3763034.1"
                     /translation="MSDPRPGLTREWLSAAPIAYFEAVDRKDLDSALDFFAEDATFTV
                     QSAHLSFAGRDEIAGMFETFFADYETIRHDVTNLVVDERVGKVSTEQNCPHVREDGTP
                     ESVYTCNFFTVRDDGRFTRVIVWIDAASPLK"
     gene            5240..5312
                     /locus_tag="AC150V_08690"
     tRNA            5240..5312
                     /locus_tag="AC150V_08690"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5273..5275,aa:Val,seq:gac)
BASE COUNT          834 a         1891 c         1993 g          883 t
ORIGIN      
        1 tgaggcgggt gctggagcgg ttcgagcggc gcaccagggc cgcggtggtg tggtcgtagt
       61 gcgcgccctc atcctgttga cggctatcac cgtgctcggg atcgggtcgg cgccggccgc
      121 ttcgagccag gtagcgggct ccgtgcgcta cctcgaagcg gttcagcagt cggacggagg
      181 cttcgccgag ggcgggcagc ccgccgacgc ggcgttgacg gcctggggtg cgctcgcgct
      241 tgccgctgga ggcggatcgg acgccgcgcg tgagcgggcg ctcgccttcc tgcggacgca
      301 cgaacgcgac gagacgggcg gtgccgcgct tgcgctcgag gtgcttgcga gggctgcgct
      361 gggcgatcgg ccggacggtc tcgcgcggcg gctgcgcgac caccgacccg gcacgctcgt
      421 caacgagacg atctggacga tcctcgcgct gcgcgcgacc gaggagcagg ttccgcgctc
      481 ctatgtgaaa gccgtgctcg cgtctcagca cgctgcaggc ggctggtcgt gggcgaggcg
      541 tgggagcccc gactcgaacg acaccgcggc agcgatccag gcgctccgcg ccgtcggtgt
      601 cacgggcgcc ccgatcaagc gcgcgctcgc cgcgctccgc ggcttccaga accgggacgg
      661 tggcttcggg ctcgtcaagg ggcgtgcctc ggacgttcaa tcgaccgcgt gggcgatcca
      721 ggcgtttctc gccgccggtg ccaagcccgg caagccggcg ttccggtacc tgtcgcgcct
      781 caggcgcccg gacggcagct accgctattc gaccgcgtac ggaacgacgc cggtctgggt
      841 caccgcgcag gtcgtacccg ccctggcggg acgcccgttc ccgttggcgg gccgatgagc
      901 gagcatgacg cgggccgccc acggcggccc gggtagcatc ggcccgatgt cagcggcgct
      961 cccgaccgag tacctcgccg atctcaacga gccgcagcga gaggctgtgc tggccgtcga
     1021 gggcccggtg ctggtcgtcg ccggagccgg ttcgggcaag acgcgcgtgc tcacgcatcg
     1081 cgtcgcgcat ctgatccgcg cctgcggcgt gaagccgaac gagatcctcg ccatcacgtt
     1141 caccaacaag gcggccggcg agatgcgcga gcgcctggag cggatgctcg ggcacacggc
     1201 gcgcgccatc tggatcctca ccttccattc cgcgtgcgga cgcatcctga ggcgggaggc
     1261 ggcgcggctc ggctatcgct cgaacttcac catctacgac caggccgacc aggtgcgcgt
     1321 cgtgaaggcg tgtctcgagg agctggggcg tgatccgaag aggttcacgc cgcgcggaat
     1381 ccacgtccag atctcgaacg cgaagaaccg gctcgtcggg ccggacgagt acatggcccg
     1441 ggtctcctcg ttctacgacc agaccgtcgc cgaggtgtac gagctgtatc agcgcaagct
     1501 gttcgaatcc aacgcggtcg acttcgacga catgctgatg ctgaccgtcg atctgctcga
     1561 gcgcttcccg gaggcgcggg ggcactggca gagggccttc cgatacgtcc tcgtcgacga
     1621 gtaccaggac acgaaccacg cgcagtaccg gttgctgcgt ctgctcgccg ccgagcacgg
     1681 caacgtcttc gccgtgggcg accccgatca gtcgatctat gcgttcaggg gcgccgacat
     1741 ccgcaacatc ctcgagttcg agcgcgactt cgagggcacg cgggtgatcg ccctggagca
     1801 gaactaccgc tcgacgaacg cgatcctgtc ggccgccaac ggggtgatcg agcacaaccg
     1861 cgggcggaag ccgaagcgcc tctactccga cctcggagag ggcgagccgg tgcaggtgat
     1921 cgaggtcgag gacgagcacg ccgaggcgcg cttcgtcgcg gccgagatcg cccgtctcgt
     1981 cggggacggc tggtcggcga gcgagctcgc tgtcttctat cgcacgaacg cccagtcgcg
     2041 ggtgctcgag gacgttctcg tgcgccagga cgttccctac caggtgatcg gcgggccgcg
     2101 cttctacgag cgcgccgaga tcaaggacgc cgtggcgtac ctgacggtgc tcgacaacac
     2161 gcaggacgcg gtggctgtga tgcggatcgc aaaccgccct cgacgcggca tcggagacac
     2221 gtcgatccag cgcctcgtct cccacgccga gacgctcggc atctcgctct gggaggcgct
     2281 cgccgacccc ggggccgccg ggctcgccac tgcgcccgcg aaggcagtcc gcggcttcca
     2341 cacgttgatg cagtcgctcc agtcagcggc gcaggagctc gagatcgacg agctgatcga
     2401 ggccgtgctc gagcgctccg gcacgatcga cgcgctcgag gcggagcgga cgatcgaggc
     2461 ccgcgggcgg atcgagaacc tgcaggagct cgtcggggtc gcgcgcgagt accgggagac
     2521 gcgtgacgag ccgaccctct cggggtttct gcaggagatc tcgctcgtct ccgatcagga
     2581 tgccctgcgc gacgacgaca ccggcgtcgt cacgctgatg acgatccaca acgcaaaagg
     2641 tctcgagttc cgcggcgtct tcgtgatcgg gatggaggag ggcatctttc cccactcccg
     2701 ctccatcgag gacaacgagg tcgaggagga gcggcgcctc tgctacgtgg ggatgactcg
     2761 ggcgatggag cggctcacgc tgacccacgc gatggcgcgc tccctgttcg ggcggcgcga
     2821 ctacaacctt gcgtcgcgtt tcctcgacga gcttcccgcg tcggtcgagc gcgagcggct
     2881 gcggccgtcc tcgtggtcgg gctacgcggc ctcgccgcgg caggtcgctc cgcaggatcc
     2941 cgtcgacctg cctgcgctgc agaccggcga ctcggtcgag cacggctcgc ttggcgaagg
     3001 ggtcgttacc cggatggagc cgggcggcat cgtcaccgtg cggtttgcgc aggacgggac
     3061 ggagcgccgt ctgatgctcg agtacgcgcc gctgcggaag gtcggttgat ggcgttccgc
     3121 gtccgtcgcg tccgcgacct cgacgagtac cgtgcggcgc tcggcgtgat cggccactac
     3181 ttcggctgga acccgagcga ggaggacgcc gagcgtttct cgcgccacct gccggtcgag
     3241 cgaatgcacg cggcgtggga gggctcggaa atcgtggccg gcaccggcgc gatcccgttc
     3301 gagctgacgg ttcctggtgg cccggcgccc tgtgcaggcg tgactgtggt ggcggtccgg
     3361 ccgacgcatc gccgccgcgg cctgatgcga cggctgatgc tggcccagct ggcggacatc
     3421 cgtgagcgtg ccgagccgat cgcggcgctg tgggcctcgg aggagacgat ctacggccgc
     3481 tacggctacg gcctggccga gctgctgcag atcgagatgc ggctgcctcg cgagtacacg
     3541 cgtctacgcg gcgagagcga tccggtgggc acggtgcggc tcgtcgacca cgacgaggcc
     3601 cttcgcacgt ttccgcgcgt ctacgagcgc gttcgccgga acaccgtcgg cttcctctct
     3661 cgctcgcggg aatggtggga tctgcgccgc ctcgacgacg cgcccgagcg ccgccgcggg
     3721 atgggccccc tcaatcgggc gttgctcgag atcgacggac gaccggccgg ctacgccctc
     3781 taccgtgtca agagcgagtt cgagggcgcg gtgaatatgg gtcacgtcca ggtgatcgag
     3841 gcacttggcg tgtcggcgga ggcaacgcgc gagatctggc ggttcgcgct cggcgtcgac
     3901 tggctcggcg aggtgcggac ggcgctgctt cccctcgatc atgcgctgct ccagctcgtg
     3961 acgcacccca accggatgcg ctggcagatc gccgacggga tctggctgcg gccgatcgac
     4021 gtcggggccg ccctcgccgc gcgctcgtac tccggtgatg gtcgcgtgac gctcgagatc
     4081 gcggccgacc cgcagttccc cgacaacgtc cagcggtaca cgatcgaggg tggggacgtg
     4141 cgcaggaccc gtcggcgccc cgacgtgcga ctcgacgtcc aggcactcgg gtcggcctac
     4201 ctcggcggct tcacgttcgc cgcgctggct gccggcggcc gggtacagga ggtgacgcgt
     4261 ggcggcctcg cccgcgccga cgcgctcttc cgcaccgacc ggctgccgtg gacgcccgag
     4321 ctcttctaga catcggcagt tgcccgcccg gttgtaggat cgtgagatgg cgcgcttccg
     4381 cgaggtgatc ggcgtgtcgc tgccgctcga ggcatcgttc gcgtacgtcg ccgacttcac
     4441 gacggcggcg gagtgggatc ccggaatcct cgagtcgcga gccgtggacg agggcgagcc
     4501 gcgcgtcggc agccgctacg acgtcgtggc caactttcgc ggacgcagca tcccattccg
     4561 ttacgagatc ctccagctcg agcccggtcg gcgaatcgtg atccgcggcg aaggtgacaa
     4621 ggcgacctcg gacgacacga tcgagttcga gccggcgggc cccggtacga gaatcagcta
     4681 cgaggccgag ctgaagatga aaggcatctg gcgtgcggcg gagccgtttc tcggcggaac
     4741 cttcgcgacg atgggcaaga aggcgctcca cggtctgaag gaacagctcg accggcgtgg
     4801 cgccgaggcg gcgtgagcga cccgcggccg ggcctgaccc gcgagtggct ctccgcggcc
     4861 ccgatcgcct acttcgaggc cgtcgaccgc aaggatctcg actcggcgct cgacttcttc
     4921 gccgaggacg cgacgttcac ggttcagagc gctcacctga gcttcgccgg tcgcgacgag
     4981 atcgccggca tgttcgagac gttcttcgcc gactacgaga cgatccggca cgatgtcacc
     5041 aacctcgtcg tcgacgagcg ggtcgggaaa gtgtcgacgg agcagaactg cccgcacgtg
     5101 cgggaggacg gcacacccga gagcgtgtac acctgcaact tcttcaccgt ccgcgacgac
     5161 ggccggttca cgcgcgtgat cgtctggatc gacgccgcct cgccgctcaa gtaaccaccc
     5221 gctagaatcc ggctccccgg ggcggttagc tcagttggga gagcaccagc tcgacaagct
     5281 gggggtcact ggttcgagcc cagtaccgcc cacctccaaa acccgctgga aaacggcggg
     5341 ttttcttttg ggcgaagtgc gccgggaatg gtcgcctggt tgcaagatcg gccctggtgg
     5401 ttgcacttcg ggccgagtcc ctgggatccg gctcgcccga tggtcttctg gctatctaga
     5461 gagggtcatc cctccgcgcg tcgtcattct gaactggcgc ggttactcgc tgagcgcgtc
     5521 ggccgagtac acgtcctcca tgttcgaggc cggaagaact ccgagagcgt tggcagtggc
     5581 atcgacggct tctgccgcct c
//
LOCUS       DBNGNK010000296         3317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_482058,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000296 DBNGNK010000000
VERSION     DBNGNK010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_482058"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC150V_08695"
     CDS             <1..614
                     /locus_tag="AC150V_08695"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HIR3763035.1"
                     /translation="VAALLEYGVHTIVDLRMPSELEPDWPRTDGVTALHAPFCPDTSP
                     ADWAALDRVGLAAGDEVEATRAVYVEMLERSREYVATAVATVAAAAASGTVLVHCQGG
                     KDRTGLLSALLLELADVERAAIAADYAYSRVALAEHLDAWIDEASDDRERELRVLVGA
                     TPAPSMLGVLADLDDRYGGAHEFLLESGVHAEALTFLRALLRP"
     gene            611..1522
                     /locus_tag="AC150V_08700"
     CDS             611..1522
                     /locus_tag="AC150V_08700"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-dimethylallyltransferase"
                     /protein_id="HIR3763036.1"
                     /translation="MTAALRGVEVVAIFGPTAAGKSAVAEGLADLLGTDVVSADAMQV
                     YRGLAILTNQPSRPTRLVGIRALSDEMSVGEYADLAHAEIDSLVEAKGSAIVAGGTGL
                     YLRAAIADLEPPPAPTPEARRRWEAVYDSDARAAHALLAVRDPVAASLVHSNDRRRVV
                     RALELTELSASLVPGDDRLWSAETRRPTLVVGLDVPAEELDRRIAERTRTMFRLGVVD
                     EVNAACAGVVSKTAEKALGLREIADSSKEEAIELVISRTRRYAAYQRKWMRRVPGLVL
                     VDGDRATRDIVAEVAARAEAAEPYGSK"
     gene            1560..2087
                     /locus_tag="AC150V_08705"
     CDS             1560..2087
                     /locus_tag="AC150V_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763037.1"
                     /translation="MRKTLIALAAAAALAAAGCGGNGGGDSLSKEEYQAAVADVGQVL
                     EAAFDEIAQEATSISGDVGSLDEAGEAFDSLAASIDDGSEALEQAASDLDELDPPDEA
                     KDATGELANGLRALSGDLDELSAALEGGDFSRILQLGRELQEIASSDAGKQIEGAIEE
                     LKSLGFDVEGDGTEG"
     gene            2090..3226
                     /gene="dapF"
                     /locus_tag="AC150V_08710"
     CDS             2090..3226
                     /gene="dapF"
                     /locus_tag="AC150V_08710"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR3763038.1"
                     /translation="MRFAKWHALGNSYLIVERAGRTEYLDPETAASLCDPQRGIGADG
                     VLEVVTSDAESAEIVIWNPDGTTAEMSGNGTRIAAAWLMERSAATEAVIRSAGRAVHA
                     RRRDDGMIEQELGRVAVSPQESIDVYGNPVQVIPVNVGNPHAVLPCREPSREVLLRLG
                     PLVETHPRFPERTNVQLAHRDGPNDVSALVWERGAGETAASGSSAVAVAAAAVESDWC
                     RSPVTVHMPGGTLEVVLEDDSVTLIGPAEEICRGELRAGSGAEVGSIAWADLTVDDAD
                     RLRDFYAAVAGWEPHGVDMGGYEDWTMTASGQPVGGVCNARGPNEDLPPVWLLYITVP
                     DLAVALENARSRDGEVLREPEPGRPYAVLRDPAGAAFALFEARS"
     gene            complement(3223..>3317)
                     /locus_tag="AC150V_08715"
     CDS             complement(3223..>3317)
                     /locus_tag="AC150V_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008238929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR3763039.1"
                     /translation="QCALIVGDRSASTDLKTSLNETLREFEIAV"
BASE COUNT          531 a         1091 c         1206 g          489 t
ORIGIN      
        1 gcgtcgcggc gctcctcgag tacggcgtcc acacgattgt tgatctccgc atgccgagcg
       61 agctcgagcc ggactggccg cgcaccgacg gcgtcacggc tctgcacgcg ccgttctgcc
      121 ctgacacgtc gccggcggac tgggccgcgc tcgaccgtgt cggcctcgcg gcgggtgacg
      181 aggtcgaggc cacccgcgcc gtctacgtcg agatgctcga gcggagccgc gagtacgtgg
      241 cgacggcggt cgccactgtg gccgctgcgg cggcgagcgg taccgtgctc gttcattgcc
      301 agggcgggaa ggaccgtacc ggcctgctca gcgcgctctt gctcgagctc gccgacgtgg
      361 agcgggccgc gatcgcggcc gattacgcgt acagccgcgt ggcgctcgcc gagcacctcg
      421 acgcgtggat cgacgaggcg tccgacgacc gggagcgaga gctccgcgta ttggttggtg
      481 cgacgccggc accgtcgatg ctcggcgtgc tcgccgatct cgacgaccgc tacgggggcg
      541 cacacgagtt cctgctcgaa tccggtgtcc acgcagaggc actcacattc ctccgcgcgc
      601 tactgcggcc gtgaccgcgg cgttgcgagg tgtggaggtg gtggcgatct tcggccccac
      661 ggccgcggga aagtccgctg tcgccgaggg acttgccgac ctgctcggaa cggacgtcgt
      721 ctccgccgac gcgatgcagg tgtatcgagg tcttgccatc ctgaccaacc agccgtcgcg
      781 accgacgcga ctggtcggca tccgggcgct ctccgacgag atgtcggtgg gcgagtacgc
      841 ggatctggcc cacgccgaga tcgactcact ggtcgaagcg aaaggctcgg cgatcgtcgc
      901 cggcgggacg ggcctctacc tgcgggcggc gatcgcggat ctcgagcctc cgccggctcc
      961 gacgccggaa gcacgcaggc gttgggaggc cgtgtacgac tccgacgcgc gggccgcgca
     1021 cgcgctgctc gccgtgcgcg atccggtcgc ggcctcgctc gtgcactcga acgaccgccg
     1081 gcgggtagtc cgagcactcg agctaacgga gctgagtgcg tctctggttc ccggggacga
     1141 ccgtctctgg agcgcggaga cgcgccggcc gactctggtc gttgggctcg acgtgccggc
     1201 agaagagctc gaccgccgca tcgcggagcg cacacggacg atgttcaggc tcggagttgt
     1261 cgacgaagtg aacgcggcct gcgcgggcgt ggtatcgaag acggccgaga aggcactcgg
     1321 actgcgtgag atcgcagact caagcaaaga agaggcgatc gagttggtca tctcgcgcac
     1381 ccgacgctac gccgcatacc agcggaagtg gatgcggcga gtgccgggac tcgtgctcgt
     1441 ggacggagac cgcgccacgc gtgacatcgt cgctgaggtc gccgcgcggg ccgaggccgc
     1501 agagccatat ggctcgaagt agactcggcg cgccacgtca cgacagagag gggcaacccg
     1561 tgaggaagac cttgatcgcg cttgccgcgg cggcagcact cgcggcggcc ggctgcggcg
     1621 ggaacggcgg cggggactcg ctttcgaaag aggagtacca ggcggcggtg gccgacgtcg
     1681 gtcaggtgct cgaagctgcc ttcgacgaga tcgcccaaga ggcaacatcg atcagcggag
     1741 acgtcgggtc gctcgacgag gccggcgagg ctttcgattc gctggctgct tcgatcgatg
     1801 acggctccga agcgctcgag caggccgcaa gcgacctcga cgagctcgat cctcccgacg
     1861 aggcgaagga tgcaaccggc gagctcgcga acggcctgcg cgcactgtcg ggagacctcg
     1921 acgagctcag cgctgcactc gagggcgggg acttctcgcg gatactccag ctcggccgtg
     1981 agctccagga gatcgcgtcg tcagatgcgg gcaagcagat agaaggtgcg atcgaagagc
     2041 tgaagtcgct gggcttcgac gtcgaggggg acggaacgga gggctagagg tgcgcttcgc
     2101 caaatggcac gcgctcggca acagctacct gatcgtcgaa cgagcgggac ggaccgagta
     2161 cctcgacccg gagactgctg cgagcctgtg cgacccgcag cgcgggatcg gcgccgacgg
     2221 cgtgctcgag gtcgtcacct cggacgccga gtccgccgag atcgtcatct ggaatccaga
     2281 cggcacgacg gcggagatgt cgggcaacgg gacacgcatc gccgccgcgt ggctgatgga
     2341 gcgctcggct gcgacggagg cggtcatccg gtcggccggt cgagccgtac acgccaggcg
     2401 tcgcgacgac ggcatgatcg agcaagagct cggccgcgtc gccgtgtccc cgcaggagtc
     2461 gatcgacgtg tacggcaacc ccgtgcaggt gatcccggtg aacgtcggaa atccgcacgc
     2521 cgtgctgcct tgccgcgaac cgagccgcga ggtgttgcta cgcctcggtc cgttggtgga
     2581 gacgcatccc cgcttcccgg agcggacgaa cgtgcagctt gcgcaccggg acggaccgaa
     2641 cgacgtctcg gccctcgtct gggagcgggg ggcgggggag actgctgcgt ccggatcctc
     2701 ggccgtcgcg gtcgccgccg cagcggtcga gagcgactgg tgccgatctc ccgtcaccgt
     2761 gcacatgccc ggcggaacgc tcgaagtcgt gctcgaggac gattccgtga ccctcatcgg
     2821 cccggcggag gagatctgcc gcggggagct tcgcgccgga tcgggagcag aagtcgggtc
     2881 gatcgcctgg gccgacctga ccgtcgacga tgccgatcgg ctgcgtgact tctacgcggc
     2941 cgtcgccggc tgggagccgc atggtgtcga catgggcggg tacgaggact ggacgatgac
     3001 cgcctccggc cagccggtgg ggggtgtctg caacgcgcgc gggccaaacg aggacttgcc
     3061 tccagtctgg ctcctgtaca tcaccgttcc cgacctcgcc gtcgccctcg aaaacgcccg
     3121 tagccgggat ggtgaggtgc tacgcgagcc cgagccaggc cgaccgtacg cagtgctgcg
     3181 cgatccggca ggcgccgcct tcgctctctt cgaggcgcga agctagacgg cgatctcgaa
     3241 ctcgcgcagt gtctcgttca gcgacgtctt gagatccgtc gacgcactac ggtccccgac
     3301 gatcagcgca cactgga
//
LOCUS       DBNGNK010000297        13836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1340340,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000297 DBNGNK010000000
VERSION     DBNGNK010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 13836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13836
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1340340"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC150V_08720"
     CDS             <1..207
                     /locus_tag="AC150V_08720"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR3763040.1"
                     /translation="EVESLPFADASIDVVISNGVIDLVPDKDAVFSEIYRVLRPGGRI
                     QLADVTIQNPVSEEGRRDIDLWTG"
     gene            complement(456..1337)
                     /locus_tag="AC150V_08725"
     CDS             complement(456..1337)
                     /locus_tag="AC150V_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015622712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3763041.1"
                     /translation="MDVKGALFSLPDGRELRYRVTESERGASVLLLTGTPSAGIAPRA
                     AIAAAAERGLRLVTYSRPGYEDSTRLPERSVADCVPDVEALAVGLELEPLYVVGWSGG
                     GPHALACAALLPGRIRAAATLAGVAPWDADGLAWLDGMGPENVDEFRAALAGEKALRS
                     ALAEQRAELAQVTGDQVAEALGGLVADVDRSALDGELADYIATGFRKAVSTGIDGWVD
                     DDLAFARGWGFSLDAIGVPITVWQGDEDRMVPFAHGEWLSERVPGAAPRLLSGEGHIS
                     LVERFGDIVDDLVARGG"
     gene            1386..2477
                     /locus_tag="AC150V_08730"
     CDS             1386..2477
                     /locus_tag="AC150V_08730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763042.1"
                     /translation="MTAVATDAAAERRDALVERLFSSTIGAFDLAGVYVGERLGLYRI
                     LADQNPMTARELAGAAEIDERYALEWLEHQAASSILEAEETAGTWRFRLPEGHDEALL
                     DETSLNYVTPFSRLLVACLRPIDALLDAYRTGEGVAYGEYGADLHEGQAAFTRPMFLT
                     LLAQEWLPSVRDIHERLLADPPARVADVACGCGWSSIGMAQGYEKIHVDGLDLDEPSI
                     ELARRHLAATRLGDRVTFHARDAADPGLSGRFDLVTMFESLHDMSYPVRVLEACRGLL
                     ADGGVVLVGDELTGEAFDPQAGDLERLYYGFSILHCLPVGMVGDGAAGTGTVMRAETL
                     RRYASEAGFKTVEVAPIENDFWRFYLLRR"
     gene            2514..3215
                     /locus_tag="AC150V_08735"
     CDS             2514..3215
                     /locus_tag="AC150V_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR3763043.1"
                     /translation="MSAERHQLAVTERGESERGSRRVRRMRKEGLIPGVLYGDGHTRA
                     IVVEEQALRTALTTSGGLNAILDVIVDGEETPHYAVLKDYQQHPIRGTITHFDFHEVR
                     LDRAIQAVTIVHLVGESPGVKVGGVLQQVTRELNVEALPMEIPESIEASIEGLEIGDA
                     LRLEDLATIDGVIFLDDPHDTVIASVTAPRVEEEPEEEEEELEEGEEGELAEGEEGEP
                     TAEGEPAEGDQPEEE"
     gene            3228..3821
                     /gene="pth"
                     /locus_tag="AC150V_08740"
     CDS             3228..3821
                     /gene="pth"
                     /locus_tag="AC150V_08740"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR3763044.1"
                     /translation="MRLLRRGEPAPTLDLLVAGLGNPGPEHAADRHNVGWMVVDELAR
                     RREGSFKAKFRGRVADVRVGAAKLVLLKPETYMNESGASIRAATRFYKVPPEGLLVVH
                     DDVDLPVGRLQARLGGGLAGHNGLRSIAQELGSQEFLRLRIGVGRPERGDRRPVADYV
                     LSSFSSEDDVEAIVGRAADAVEALAADGLDEVQRRFN"
     gene            3870..6245
                     /locus_tag="AC150V_08745"
     CDS             3870..6245
                     /locus_tag="AC150V_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR3763045.1"
                     /translation="MDKTRAPAAEASPPLPADDLAVLESIERRVRWLATRIVDYANRE
                     RPKDDALKVGGHQASSASMVSLMTALYIVDLQATDRVSVKPHASPVLQALEYLLGRLD
                     RSYLTRLRDLGGLQPYPSRTKDPYPVDYSTGSVGLGAAAPLFGALADRYVQHRLGVGT
                     GGRFISLLGDAELDEGNVWEAVVEPHTRGLGNVLWIVDLNRQSLDRVIPIINATRLEA
                     QFAAAGWQVLTLKYGRRLRDAFARPNGDLLRRRIDEMPNQLYQSLFGASEEVATDVLL
                     RDLSGDDRDRLGTLLAEYAGRVGALVQDLGGHDLADILAALDAARGEPERPTVVFAYT
                     VKGYGLPIAGRPMNHSALLTGDQIDDLRADVGLSLDSEWDAFPPDSSEGRLIAQARAR
                     LDRSVRSAPKRVGVPATIGGEPPARASTQEAVGRLLLALSREEELAERIVTVSPDVSV
                     STNLGGWINKVGVFGLEDEPAYEGPDVQPLSWSVSSSGRHIELGISEMNLFLALGQLG
                     MTAEYQGERLFPIGTLYDPFVVRGLEALVYGAYSASRFVVLGTPSGVSLSREGGAHQS
                     TITPGIGTELPGVSYCEPAFARELEWQLLEGLSQMQGDDGDILYLRLSTKPIEQAPFT
                     ELCRMRGEDTVRADVLAGAYWLREPVVAEDAVVLATCGSMVPEVLQAADALEAEEGIT
                     AGVLCLSSPGRLYRDWRNARLVHLADLAAVRRPSHLERLVPPAVRGLPVVSVIDGASH
                     ALGWLGGALGTRVVTLGVDQFGQAGSQPAVYAAYGLSPDAIVTAALIALEP"
     gene            complement(6249..7034)
                     /locus_tag="AC150V_08750"
     CDS             complement(6249..7034)
                     /locus_tag="AC150V_08750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3763046.1"
                     /translation="MSELDRFRLDGKVALVAGGSGGIGVRLCGALADVGAGVAVVGRR
                     AEGLEAASRAVSEAGGEALAIAADMTKEDEVRRAVKEASTHFGRLDILVNAVGGGAGD
                     VLHAAEDYPEDAWDWIMDINLRSAFLTSREAARVMIEAGRGGRILNISSVRGQLGIHN
                     GFTAYVAAKGGMNALTRQHATEWAKHSITVNAISPTFVATAQVASILADDSFRESLLA
                     RIPLSRIAETDDLVGATLFFCSDASSFVTGQVLTLDGGLTACQ"
     gene            complement(7031..7474)
                     /locus_tag="AC150V_08755"
     CDS             complement(7031..7474)
                     /locus_tag="AC150V_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3763047.1"
                     /translation="MTIRVRVNGIDHELDVRADETLLTVLRRDLGLLSVRETCSIGAC
                     GTCTVLLDGEPVSGCLLLAPLAEGRSVETVEALDEHDPVGRAFADAHAYQCGWCTPGM
                     ILTAKQLLAENPSPTEKEITTAMSGNLCRCGSYVKIVDAVRRAAS"
     gene            complement(7471..9543)
                     /locus_tag="AC150V_08760"
     CDS             complement(7471..9543)
                     /locus_tag="AC150V_08760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3763048.1"
                     /translation="MTESSVPGSARYTVDLELPDMLHARIVRSPLPHGRVERVDVTAV
                     PPEVVTLTPDDIRGLAAYGAQLADQTILATDRVRFVGDPVAAVAAETPDEARAAAWLV
                     EVAAEELPAVFDPLEACAPDAPLIHERHAVSGSAAAYFGLRPQPDTNVCHVFSIRHGN
                     TEAAFAEANVIVERTYRTSGAQHAAMEPHATVARWQDGRLEVWTGTQTPFNVRSDLAG
                     IFGLEEEHVRIVVPPMGGSFGTKTFTRIEAITAALARKAEQPVKLVLDRDEEWLTNGR
                     HPSVVRVKLGARRDGTLVAKEAECWVDTGAYADCGPGVAQKLGFSVPGPYRIPNVRVD
                     SRCVYTNLPPNGAFRGYGAMQAVWASERCMDALAESLGMDPLELRLRNVLRDGDVYCT
                     GETVHDVHFDECLRAAAAAVDWESGRDGKGLCVLLKGMQTPSRAAIAIEAEHDGSYTL
                     RCATTEVGQGARRTLCVLAAAELGVEADRIRFPPPDTDAVPYDTRTTSSRSTYMMGRA
                     ITAAVQALRESDDGIGVGEIANEGGLDPHTGQGVASTHWHQGAAAAEVSVDEKTGRYE
                     IARLHAAVYAGRVVNRVGAELQNEGSMIMGLGTTLFEAVDFEDGNVTNANLSDYQVPA
                     FADVPAGMTHELIEHEGADVHGLGETAVPPVPAAVGNALASLGIDIPNVPITFEAVLD
                     AVEEREDS"
     gene            complement(9540..10331)
                     /locus_tag="AC150V_08765"
     CDS             complement(9540..10331)
                     /locus_tag="AC150V_08765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3763049.1"
                     /translation="MPTWLAPRTLDEALAAKAELGDEATVVAGGTFLAILLNQRLLAP
                     ESLLALRDVPGLDLIDAGAELELGALVTHRAVERAEQVRADWPALAYTFSVVASPRVR
                     NQATVGGVLADADYASDPPAMLAALGARAVVRSLRGEREVSIENLIVGHYETSLEPDE
                     IITSVRVPRPDRAAYRKFRSRSHEDRPCVSVAAAYRAGELRVAVGAVAERPQLFPEHC
                     ALGDPAEIGLRYAATIEPISDQRGSASYRRRVIAVEVRRAVEELS"
     gene            complement(10341..11153)
                     /locus_tag="AC150V_08770"
     CDS             complement(10341..11153)
                     /locus_tag="AC150V_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR3763050.1"
                     /translation="MTLLALSGSEAADVSRAGGKGASLAVMLARGFAVPPGFVVAAET
                     LEASVDGEALRALARALDHERAHALVLAAEPPREQIRRAYSELGGGRVAVRSSACAED
                     SEAASFAGQQETYLGVLGDDDVCARVVDCWASFFSERALFYRSQKGSLDDLAMAVVVQ
                     RMVEPDKAGVIFTVDPVQRRRDRMIVEAVLGLGEQVVSGEVTPDHYVVDRSGARKRER
                     LVGDGVLTDEELADLARLGAELEAHFGMPQDVEWAIAEGQVFLLQSRPVTTV"
     gene            complement(11150..11869)
                     /locus_tag="AC150V_08775"
     CDS             complement(11150..11869)
                     /locus_tag="AC150V_08775"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR3763051.1"
                     /translation="MTRLIDLSMPVHRDMLTFPRVPPPTLLMYESWEEFADRIGASEH
                     GADWLTASYLVIQNDHVGTHVDAVKHLRGPDAPGPEGIPLEYCYSDAVCLDFTHKESG
                     TWIMPAEIDEKLSEAGYTLKPKDIVLIHTGAGAYNTEERYRTDHCGMTAEATRHLIDQ
                     GVLMMGIDAITFDPPVWSMFEHKRFWEAHRVMIDTDYWHIENMMNLDQLPTHGFKLSV
                     LPIAWQGTTAAPVRAIAILDE"
     gene            complement(11919..13655)
                     /locus_tag="AC150V_08780"
     CDS             complement(11919..13655)
                     /locus_tag="AC150V_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR3763052.1"
                     /translation="MSTTASEPVLDSFLGSDEFPVEWAEGERELFWVLDDLHCPNPLS
                     PMFFDIGGWWLTCDHMFRRFGTPFASDWIAKNVNGYLYTAATAADPSVLAEGAEYEAR
                     YVPRSPRSADYAERIGAYMGAVLPHYAGNFLDWWSDRLRPEIERNFAYLDGYDMDRAD
                     LVELAVLLEDAIDIHDRHWKIHWMLNFAQFTATMGLSSAIERVKGEADPALVGRLQSS
                     VADRNWDSIEALWEMKNEIKDDAELRSAFEGLGAAEAMAALSASERGRGFLDERLTPY
                     RLEFGNKAIWSHEFVYPTWRENPAPILEAIRGYLETDYDYPATVRAVRDDLEAAKQEL
                     LHDVPAGEPRKELESALELSLRMNPLTPDHHFFIDQGTNARVRLVLVAMGRKLTEAGV
                     LGDPEDVVFLRYNELRALVGAPRAFDAAALVSDRRDAREAQASVRPPDWIGTATESQL
                     AFPYYSLWGFPEKFHRGPPEKAGEVHGLAASPGVVEGTARVVTSPDEFDSVQEGDIVV
                     CVMTNPGWVVLFTKIHGLVTDAGGVAAHPAVVSREFGIPAVVGTSNATAAIETGDRVR
                     VNGTTGVVEILG"
BASE COUNT         2132 a         4865 c         4670 g         2169 t
ORIGIN      
        1 gaggtcgaat cgcttccgtt cgccgacgcc agcatcgacg ttgtcatctc caacggcgtc
       61 atcgacctcg tccccgacaa ggacgccgtc ttctcggaga tctaccgggt gctccgtccg
      121 ggcggtcgca tccagctcgc cgacgtgacg atccagaacc ccgtgagcga ggagggtcgc
      181 agagacatcg atctctggac cggctgaatc gcaggcgctc tgctggaagc agagtacgaa
      241 gaggttctcg agcggcacgg attcgagcgg atcgagacag gcaacctcgt cgccacgtac
      301 atgcgatcca agttcgagga cacgcgtcgc aaggcgacca agtttggggc gaacggctca
      361 accgtcaagg ccttcaaacc gtcctgacgt cgcaggccgc ccgccggctt ccgggcggcg
      421 gagccgccct ccgccgtcgg ctggcgccgc ccgcttcagc cgccgcgcgc gaccaggtcg
      481 tcgacgatgt cgccgaagcg ctcgacgagc gagatgtgcc cctcgcccga gagaaggcgt
      541 ggcgcggcgc ccggaacgcg ctccgagagc cactcgccgt gggcgaaagg gaccatccga
      601 tcctcgtcgc cctgccatac ggtgatcggc acgccgatcg cgtcgagcga gaagccccag
      661 ccgcgcgcga aggcgaggtc atcgtcgacc cagccgtcga tgccggtcga aacggctttg
      721 cgaaatccgg tcgcgatgta gtcggccagc tcaccgtcga gggcagaccg gtcgacgtcg
      781 gcgacgaggc cgccgagcgc ctcggcgacc tggtcacccg tgacctgtgc cagctcggct
      841 cgctgctcgg ccaacgctga acgaagcgcc ttctcaccgg cgagcgcggc gcggaactcg
      901 tcgacgttct ccgggcccat gccgtcgagc catgccaagc cgtcagcgtc ccacggcgcg
      961 actcccgcga gcgtcgcggc ggctcgtatc cgcccgggaa ggagcgcggc gcaagccaac
     1021 gcgtgcggcc cgccgccgga ccagccgacg acgtagagag gctcgagctc gaggcccact
     1081 gccagtgcct cgacgtccgg cacgcagtcg gcgaccgagc gctcgggcag tcgggtcgag
     1141 tcctcgtacc cgggccggga gtacgtcacg aggcgtagcc cgcgctccgc agcagccgcg
     1201 atggcggcgc gcggggcgat tcccgccgag ggggtacccg tgaggagcag cacgctcgca
     1261 ccgcgctcgg actcggtgac tcgatagcgg agctccctgc cgtccggaag cgagaacaag
     1321 gcgcccttca cgtccaccgc acgatcctac gcgccggctg ggaaccgcca agaccgcatt
     1381 gtaggatgac cgccgtggca acggatgctg ccgccgagcg acgcgacgca ctcgtcgagc
     1441 ggttgttctc ctccacgatc ggtgcgttcg accttgccgg ggtctatgtc ggggagcgcc
     1501 tcggcctgta ccgcatactt gccgaccaga atccgatgac ggcccgcgag ttggcgggcg
     1561 cggccgagat cgacgagcgc tacgcgctgg agtggctcga gcaccaagcg gcctcgtcga
     1621 tcctcgaagc cgaggagacc gccgggacct ggcgctttcg ccttcccgaa ggccacgacg
     1681 aggcgctgct cgacgagacg agtctgaact acgtcacacc cttctcacgc ctcctcgtcg
     1741 cttgcctccg gccgatcgac gcgctactcg acgcctaccg aaccggcgag ggcgtcgcct
     1801 acggcgaata cggcgcggac ctgcacgaag gccaggccgc tttcacccgg ccgatgttcc
     1861 tgacgctgct ggcgcaggag tggctgccgt ccgtccggga catccacgag cgcctcctcg
     1921 ccgatccacc ggcgcgagtc gcagacgtcg cctgcggctg cggctggtcg agtatcggca
     1981 tggctcaggg atacgagaag atccacgtcg acggtctcga tctcgacgag ccgtcgatcg
     2041 agctcgcgcg gcgccatctc gctgcgacac ggcttggcga ccgcgtcacc ttccacgcac
     2101 gcgacgcggc tgacccggga ctctcgggcc gcttcgacct cgtcaccatg ttcgagtcgc
     2161 tgcacgacat gtcgtacccg gttcgtgtgt tggaagcgtg ccgcgggctg ctcgccgacg
     2221 gcggcgtcgt cctcgtcggt gacgagctga ccggcgaggc gttcgacccg caggcaggcg
     2281 atctcgagcg cctctactac ggcttcagca tccttcactg cctgccggtc ggcatggtgg
     2341 gcgacggtgc ggccggcacc gggaccgtga tgcgcgccga gacgcttcgg cgctacgcga
     2401 gtgaagcggg tttcaaaacc gtcgaggtcg cgccgatcga gaacgacttc tggcgcttct
     2461 acctcctccg gcggtagcga tccggacgat tcggtccgct aggcttcgcc gccgtgtcgg
     2521 ccgagaggca tcagctggcg gtgaccgagc ggggcgagtc ggaacgaggc tcgcgccgtg
     2581 ttcgccgtat gcgcaaagag ggattgatcc cgggggttct ctacggtgac gggcataccc
     2641 gcgccatcgt cgtcgaggag caggctcttc gcactgcgct gacgacgtcg ggtggtctca
     2701 acgccattct cgacgtcatc gtcgacggcg aggagacgcc gcactacgcc gtcctcaagg
     2761 actaccagca gcaccccatc cgcggcacca tcacgcactt cgacttccac gaagtccggc
     2821 tcgaccgggc gatccaggcg gtcaccatcg tccatctcgt cggcgagtca ccgggcgtga
     2881 aggtcggcgg cgtcctccag caggtcaccc gcgagctgaa cgtcgaggcg ctcccgatgg
     2941 agatccccga gtcgatcgaa gcctcgatcg agggcctcga gatcggtgac gccctccgcc
     3001 tcgaggactt ggccacgatc gacggagtca tcttcctcga tgatccgcac gacacggtca
     3061 tcgccagcgt cactgcacct cgtgtcgagg aagaacccga ggaagaggaa gaggagctcg
     3121 aggagggcga ggaaggcgag ctggccgagg gcgaggaggg cgagcccacc gccgagggcg
     3181 agcctgccga gggcgaccag cccgaagagg agtaggagaa ccgcgctatg cggctcctcc
     3241 gtcggggcga gccggctccg acgctcgatc tgctcgtcgc cggtctcggc aacccggggc
     3301 ccgagcacgc cgccgatcga cacaatgtcg gctggatggt cgtcgacgag ctggcgcgtc
     3361 gccgggaggg ctcgttcaag gcgaagtttc gcggccgcgt cgcagacgtc cgcgtcggcg
     3421 cggcgaaact cgtattgctg aagcccgaga cctacatgaa cgagtccggc gcctcgatcc
     3481 gggcggccac ccgcttctac aaggtcccgc ccgaaggtct cctggtcgtc catgacgacg
     3541 tcgatcttcc ggtgggtcgt ctccaagctc gactcggcgg cgggctcgcg ggtcacaacg
     3601 ggctgcggtc gatcgcgcag gagctcggct cgcaggaatt cctgcggttg cgtatcggcg
     3661 taggacggcc cgagcggggc gatcgccgac cggtggccga ctacgtcttg tcgtccttct
     3721 cttccgagga cgacgtcgaa gcaatcgtcg gccgagccgc cgacgcagtc gaggctctgg
     3781 ccgccgacgg cctcgacgag gtgcagcgcc gcttcaactg accgctaggc ggcagcacgt
     3841 cccgctctct tgcgacgtac gattcgtgcg tggacaaaac ccgggctccc gcggcagagg
     3901 cttcgccgcc tcttcccgcc gacgacctcg cggtgctcga gagcatcgag cgccgagtcc
     3961 gctggctcgc gacccgcatc gtcgactacg cgaaccgcga gcggccgaag gacgacgcgc
     4021 tgaaggtcgg cggccaccaa gcgtcgtcgg cctcgatggt ctccctgatg accgctctct
     4081 acatcgtcga cctgcaggcg accgatcgcg tttccgtcaa gccgcacgcc tcaccggtgc
     4141 tgcaggccct cgagtatctc ctcggccggc tcgaccgttc ctatctcacg cggctccgcg
     4201 acctcggcgg cctgcagccg tacccgagcc gtacgaagga tccgtacccc gtcgactact
     4261 cgaccggctc ggtcggcctc ggcgctgctg cgccgctgtt cggcgcgctc gccgaccggt
     4321 acgtccagca ccgactcggt gtcggcacgg gcggccgctt catctcgctg ctaggtgatg
     4381 ccgagctcga cgaggggaac gtctgggagg cggtcgtcga gccgcacacg cgcgggctcg
     4441 gcaacgtcct ctggatcgtc gacctgaacc ggcagagcct cgaccgcgtc atcccgatca
     4501 tcaacgcgac caggctggag gcccagttcg cggccgccgg ctggcaggtt ctcacgttga
     4561 agtacggacg tcgtctgcgc gacgcgttcg cccgcccgaa cggcgatctc cttcgccggc
     4621 gcatcgacga gatgccgaac cagctctacc agagcctctt cggagcctcg gaagaggtgg
     4681 cgacagacgt cctcctccgc gatctgtcgg gcgacgaccg agatcgactc ggcaccctgc
     4741 tcgccgagta cgcgggacgc gtcggcgcgc tcgtccagga tctcggcggg cacgacctcg
     4801 ccgatatcct cgcggcgctg gacgcggcgc gcggcgagcc agagcgtcca acagtcgtct
     4861 tcgcgtacac ggtgaaggga tacggcctcc cgatcgctgg ccgcccgatg aaccactcgg
     4921 cgctcctcac aggcgaccag atcgacgatc ttcgagcgga cgtgggcctg tcgctcgaca
     4981 gcgagtggga cgccttcccg cccgacagct ccgagggtcg cctaatcgcc caagcccgcg
     5041 cgcgtctcga ccgctcggtt cgctccgcgc cgaagcgtgt cggcgtcccg gcgacgatcg
     5101 gcggcgagcc acccgcccga gcgtcgacgc aggaggccgt tgggcgcctg ctcctcgcgc
     5161 tctcgcgcga ggaggagctc gccgagcgga tcgtcaccgt gtcgcccgac gtctcggtct
     5221 cgacgaacct cggcggctgg atcaacaagg tcggggtctt cggtctggag gacgagcccg
     5281 cctacgaggg tcccgacgtc cagcctctgt cctggagcgt gtcctcgagc ggccggcaca
     5341 tcgagctggg aatctccgag atgaacctct ttctcgcgct cgggcagctc ggcatgaccg
     5401 ccgagtacca gggcgagcgg ctcttcccga tcggcacgct ctacgaccca ttcgtcgtcc
     5461 gcggcctcga ggccctcgtg tacggcgcct actccgcgtc acggttcgtc gtgctgggca
     5521 cgccctcagg agtgtccctc tcgcgcgagg gcggtgccca ccagtcgacg atcacgcccg
     5581 gaatcggcac cgagctcccg ggcgtgtcct actgcgagcc ggcctttgcg cgcgagctcg
     5641 aatggcagct gctcgaagga ttgagccaga tgcaggggga cgacggcgac atcctctatc
     5701 tgcggctgtc gaccaagccg atcgagcagg cgccgttcac cgagctgtgc cggatgcgcg
     5761 gcgaggacac cgtgcgggcg gacgtcctag ccggtgcgta ctggttgcgc gaaccggtcg
     5821 tggcggagga cgcggtcgtg ctggcgacgt gtggctcgat ggttcccgaa gtcctgcagg
     5881 cggccgacgc cctcgaggcc gaggagggca tcacggccgg agtgctctgt ctctcgtcgc
     5941 cggggcgcct ctatcgcgac tggcgcaacg cccgcctcgt ccacctcgcc gatctggcgg
     6001 ccgtgcggcg accctcgcat ctcgagcgtc tcgttccgcc tgcggtgcgc ggccttcccg
     6061 tcgtcagcgt gatcgacggg gcgtcccacg ctcttggctg gctcggaggc gcgctcggca
     6121 cgcgcgttgt gacgctcggc gtcgaccagt tcggacaggc ggggagccag cccgccgtct
     6181 acgccgcgta cggcctctcg cccgacgcga tcgtcacggc agcgctgatc gcgctcgagc
     6241 cgtagccctc actggcaggc ggtcagcccg ccgtcgagcg tgagcacctg gccggtgacg
     6301 aacgacgagg cgtcggagca gaagaagagg gttgccccga cgaggtcgtc ggtctcggct
     6361 atccggctca gcggtatccg ggcgagcaga ctctcgcgga agctgtcgtc ggcgagaatc
     6421 gaggcgacct gcgcggtggc gacgaaggtc ggcgagatcg cgttcaccgt gatcgagtgc
     6481 ttcgcccact cggttgcatg ctggcgggtc agagcgttca ttccgccctt cgcggcgacg
     6541 tacgcggtga agccgttgtg gatgccgagc tggcctcgaa ccgacgagat gttgagtatc
     6601 cggccgccgc ggccggcttc gatcatcacg cgcgccgcct cgcgcgaggt cagaaaggcg
     6661 ctgcgtaggt tgatgtccat gatccagtcc cacgcgtcct cggggtagtc ctccgccgcg
     6721 tgcaggacat cgccggcacc gcctccaacc gcgttcacga gaatgtcgag gcggccgaaa
     6781 tgggtgctcg cctctttcac ggcacgccga acctcgtctt ccttcgtcat gtccgcggcg
     6841 atcgccagcg cttcaccacc cgcctccgac acagcccggc tcgccgcctc cagtccctcc
     6901 gccctgcgcc cgacgaccgc cacgccggcg cccacgtcgg cgagtgcgcc gcacaggcgg
     6961 acaccgatgc cccccgagcc ccccgccacc agagcgacct tcccgtccag gcgaaaacga
     7021 tccagctcgc tcacgatgca gctctccgca cggcgtcgac gatcttcacg taggaaccgc
     7081 agcggcacag gtttccggac atggctgtcg taatctcctt ttcggttggg gacgggttct
     7141 cagccagcag ctgcttggcc gtcaggatca ttccaggagt gcaccagccg cactggtagg
     7201 catgggcgtc ggcgaacgca cgcccaacag gatcgtgctc gtcgagcgcc tcgaccgtct
     7261 cgaccgagcg tccttccgcg aggggcgcca acagcaggca ccccgagacg ggttctccgt
     7321 cgagcagcac cgtgcaggtg ccgcacgcgc cgatcgagca ggtctcgcga accgacagca
     7381 gcccgaggtc gcgacggagg acggtgagga gcgtctcgtc ggcacgaacg tcgagctcgt
     7441 ggtcgatgcc gttgacacgg acgcgaatcg tcacgagtcc tcccgctctt ctactgcgtc
     7501 gagcaccgct tcgaaggtga tcggcacgtt cgggatgtcg atgccgagcg acgccagtgc
     7561 gttgccgacg gcggccggaa ccggcggcac agcggtctca ccaagcccgt gaacgtccgc
     7621 tccctcgtgc tcgatcagct cgtgcgtcat tcctgccgga acgtcggcga aggcgggtac
     7681 ctggtagtcg gagaggttgg cgttcgtcac gttgccgtcc tcgaagtcga cggcctcgaa
     7741 gagcgtcgtg ccgagtccca tgatcatcga cccctcgttc tggagctccg cccctaccct
     7801 gttgacgact cgaccggcgt acacggccgc atgcaagcga gcgatctcgt agcggccggt
     7861 cttctcgtcg acgctcacct cggcggcggc ggccccttgg tgccagtggg tcgaggcgac
     7921 accctgtccg gtgtgcgggt cgagcccgcc ttcgttcgcg atctcgccga cgccgatccc
     7981 gtcgtcgctc tcccgcaacg cctgcacagc ggcggtgatc gcacgtccca tcatgtacgt
     8041 ggaccggctc gacgtcgtgc gagtgtcgta cggcacggcg tcggtatcgg gcggcgggaa
     8101 ccggatccgg tcggcttcca ccccgagctc cgcggcggcg agcacgcaca gagtgcggcg
     8161 ggcaccctgg ccgacctccg tcgtggcgca gcgcagcgtg taggagccat cgtgctccgc
     8221 ttcgatcgcg attgccgccc tgctgggcgt ctgcattccc ttgagcagga cgcagaggcc
     8281 ctttccgtcg cgtcccgact cccagtcgac agccgcggcc gccgcccgca gacactcgtc
     8341 gaagtggacg tcgtgaaccg tctcgccggt gcagtagacg tcgccgtcgc gcaggacgtt
     8401 gcgcagtcgg agctccagtg ggtccatgcc gagcgactcg gcgagggcgt ccatgcaccg
     8461 ctccgaggcc cagacggcct gcatcgcgcc gtagccgcga aaggcgccgt tgggcggcag
     8521 gtttgtgtac acgcagcggg agtcgacgcg tacgttggga atccggtacg ggccgggcac
     8581 cgagaacccc agtttctgcg ccacacccgg gccgcagtcg gcgtaagcac cggtgtcgac
     8641 ccagcactcg gcctccttgg ccacgagcgt tccgtcgcgt cgggcgccga gcttgacccg
     8701 gacgacggag ggatggcgtc cgttcgtcag ccactcctcg tcgcggtcga ggacgagctt
     8761 gacgggctgc tccgcctttc gcgcgagagc cgctgtaatc gcctcgatcc gagtgaacgt
     8821 cttcgtgccg aaggatcccc ccatgggcgg cacgacgatg cgcacgtgct cctcttcgag
     8881 tccgaagatg ccggcgaggt cgctgcgcac gttgaacggc gtctgcgtgc cggtccacac
     8941 ctcgagacgg ccgtcctgcc agcgggcaac cgtcgcgtgc ggctccatcg cggcgtgctg
     9001 cgcgcccgag gtgcgatacg tgcgctcgac gatcacgttc gcctccgcga aggcggcctc
     9061 cgtgttgccg tggcggatcg agaagacgtg acatacgttc gtgtccggct gcggccgaag
     9121 cccgaaatac gcagcggcgc tgcccgacac cgcgtggcgc tcgtggatca ggggcgcatc
     9181 gggagcacat gcctccagcg ggtcgaagac cgccggcagc tcctcggctg cgacctcgac
     9241 gagccaggcg gcggcgcgcg cctcgtcggg cgtctccgca gccaccgcag cgacgggatc
     9301 gccgacgaac cgaacacggt cggtcgcgag gatcgtctgg tcggccaact gcgccccgta
     9361 agcggccagc ccgcggatgt cgtccggggt cagtgtgacg acctcggggg gcaccgccgt
     9421 gacgtcgacg cgctccaccc gaccgtgcgg caggggcgag cggacgatcc gggcgtggag
     9481 catgtccggc agctcgagat ccacggtgta ccgggccgag ccgggaacgg acgactcggt
     9541 cacgacaact cctcgaccgc gcgcctgacc tcgaccgcga tcaccctgcg tcggtacgag
     9601 gccgagccac gctgatccga gatcggctcg atcgtcgccg cataccgaag cccgatctcg
     9661 gccggatcgc ccagagcgca gtgctccggg aagagctgag gtcgctcggc aaccgctccc
     9721 acggcgaccc gcagctcgcc cgctcggtac gccgcagcga ccgagacgca ggggcggtcc
     9781 tcgtgcgagc gtgagcgaaa cttccggtac gccgcccgat ccgggcgcgg aacgcggacc
     9841 gatgtgatga tctcgtcggg ctcgagcgac gtctcgtagt gaccgacgat caggttttcg
     9901 atcgagacct ctcgctcgcc ccgcaggctt cgcacgactg cgcgcgcgcc gagggcggcc
     9961 agcatcgccg gcggatccga tgcgtagtca gcgtcggcga ggacgccgcc gacggtcgcc
    10021 tggttccgca cccgggggct cgcgacgacc gagaaggtgt aggccagtgc cggccagtcg
    10081 gcccgaacct gctccgctct ctccaccgcg cgatgcgtca cgagcgcgcc gagctcgagc
    10141 tcggcccctg cgtcgatcag gtcgagtccc ggcacgtcgc ggagggccag cagcgactca
    10201 ggcgccagaa ggcgttggtt caggaggatg gcgaggaacg ttccgccggc caccacggtg
    10261 gcctcgtctc ccagctcggc cttcgcagcg agagcctcgt cgagcgtccg gggcgcgagc
    10321 caggtcggca cggcagcccc tcagaccgtg gtcacgggcc gagactgcag cagaaagacc
    10381 tggccctccg cgatcgccca ctcgacgtcc tgcggcatgc cgaaatgcgc ctcgagctcc
    10441 gcccccagcc gggccaggtc cgccagctcc tcgtcggtga gcacgccgtc tccgacgagc
    10501 cgctctcgtt tgcgtgcccc gctccgatcg acgacatagt ggtcgggagt gacctcgccg
    10561 gagacgacct gctctcccag cccgagcacg gcctcgacga tcatgcgatc acggcgacgc
    10621 tgcaccgggt cgacggtgaa gatgacgcct gccttgtccg gttcgaccat tctctgcacg
    10681 acgacggcca tggcgagatc gtcgagcgag cccttctgcg atcgatagaa gagcgcacgc
    10741 tcggagaaga aggacgccca gcagtccacg acccgcgcgc agacgtcgtc gtcgccgagc
    10801 acgccgaggt aggtctcctg ctgcccggcg aagctcgcgg cctcggagtc ttcggcacag
    10861 gcggaggaac ggacggcgac ccgtcccccg ccgagctccg agtaggcccg gcggatctgt
    10921 tcccgcggcg gctccgcggc caggacgagc gcgtgggccc gctcgtggtc gagagcgcgg
    10981 gcaagggccc ggagggcctc cccgtcgacc gacgcctcga gcgtctcggc ggccacgacg
    11041 aatcccggcg gcacggcgaa gccgcgcgcc agcatcacgg ccaggctggc gcccttgccg
    11101 ccggcgcggc tgacgtccgc cgcctcggag ccgctgagcg cgagcagcgt cactcgtcca
    11161 ggatcgcgat cgcgcgaacc ggtgcggcgg tcgttccctg ccaggcgatg gggaggacgc
    11221 tgagcttgaa gccgtgcgtc ggcagctgat ccagattcat catgttctcg atgtgccagt
    11281 agtccgtgtc gatcatgacg cggtgcgctt cccagaagcg cttgtgctcg aacatcgacc
    11341 acaccggtgg atcgaaggtg atcgcatcga tccccatcat caagacgccc tggtcgatca
    11401 ggtgccttgt cgcctccgcc gtcatcccgc agtggtcagt gcggtaccgc tcctctgtgt
    11461 tgtacgcgcc cgcgccggtg tggatgagca cgatgtcctt cggcttgagc gtgtacccgg
    11521 cttcgctcag cttctcgtcg atctccgcgg gcatgatcca cgtcccgctc tccttgtgcg
    11581 tgaagtcgag gcacaccgcg tccgagtagc agtactcgag cgggataccc tccggtccgg
    11641 gagcgtcggg cccgcgcagg tgcttgacag cgtcgacatg ggtaccgacg tggtcgttct
    11701 ggatcacgag atagctcgcc gtgagccagt cggcgccgtg ctcggaggcg ccgatgcggt
    11761 ccgcgaactc ctcccagctc tcgtacatga gcagggtcgg cggagggacg cgtgggaacg
    11821 tgagcatgtc gcgatgcacc ggcatggaca aatcgatcag gcgggtcaat gcagctcctt
    11881 ccgttggccg cgggcgcggc agtccgcgac caccactatc acccgaggat ctcgacgacg
    11941 ccggtcgtgc cgttcacgcg cacgcggtcg ccggtctcga tcgcggccgt tgcgttcgac
    12001 gtgccgacca ccgccgggat gccgaactcg cgcgacacaa cggcggggtg ggcggcgacg
    12061 ccgccggcgt ccgtgacgag accgtggatc ttcgtgaaga ggacgaccca gcccgggttg
    12121 gtcatcacgc agacgacgat gtcgccctcc tgcacgctgt cgaactcgtc cggcgacgtc
    12181 acgacacggg ccgtcccttc gacgaccccg ggggaggcgg ccagcccgtg cacctcgccc
    12241 gccttctcgg gcggcccgcg atggaacttc tccgggaagc cccagagcga gtagtagggg
    12301 aaggcgagct gcgattcggt tgccgttccg atccagtcgg gcggacggac agatgcctga
    12361 gcctcgcggg cgtcccgccg gtccgagacg agcgcggccg cgtcgaaggc gcgcggcgct
    12421 ccgacgagcg cacgtagctc gttgtagcgg aggaacacca catcctcggg gtcgccgagg
    12481 actcccgcct cggtcagctt gcgtcccatt gcgacgagca cgagcctgac gcgtgcgttg
    12541 gttccctggt cgatgaagaa gtgatgatcg ggcgtcaagg ggttcatccg caacgagagc
    12601 tcgagcgcgg actccagctc cttgcgcggc tctcccgcgg gcacgtcgtg cagaagctcc
    12661 tgcttcgccg cctcgaggtc gtcgcgtacg gcgcgcacgg tggccgggta gtcgtagtcg
    12721 gtctcgaggt acccgcggat cgcctccagg atcggtgccg ggttctcgcg ccaggtcggg
    12781 tagacgaact cgtgcgacca gatcgccttg ttgccgaact cgagtcggta gggcgtgagc
    12841 cgctcgtcca ggaacccgcg gccgcgctcg gaggctgaca aggccgccat tgcctcggcg
    12901 gcgcccagcc cctcgaacgc agagcggagc tccgcgtcgt ccttgatctc gttcttcatc
    12961 tcccagaggg cctcgatcga gtcccagttt cgatccgcga cggagctctg gagtctgcct
    13021 accagggccg ggtcggcctc gcccttcacg cgctcgatgg ccgagctcag ccccatcgtc
    13081 gccgtgaact gagcgaagtt cagcatccag tgaatcttcc agtgtcgatc gtggatgtcg
    13141 atcgcgtcct cgagcaggac ggcgagctct acgaggtcgg cacgatccat gtcgtagccg
    13201 tcgaggtagg cgaagttccg ttctatctcc ggccggagcc ggtcgctcca ccagtcgagg
    13261 aagttgcccg cgtaatgcgg cagaacggcg cccatgtacg caccgattcg ctccgcgtag
    13321 tccgcgctcc tcggcgagcg ggggacgtac cgagcctcgt actcggcccc ctccgcaagc
    13381 acgctcggat cggcggccgt ggctgcggtg tagagatagc cgttgacgtt cttggcgatc
    13441 cagtcggagg cgaacggcgt accgaaccgg cggaacatgt ggtcgcacgt cagccaccag
    13501 ccgccgatgt cgaagaacat cggcgagagc gggttcgggc agtgaagatc atcgagaacc
    13561 cagaagagct cccgttcgcc ctcggcccac tcgaccggga actcgtcgct gccgagaaaa
    13621 ctgtcgagca cgggctcgct cgcagtcgtg gacatccgcg cctcctcgtc gtgtgcggca
    13681 atccttgacc ttttcgacgc gccaggtcaa acgggtacag tcgcgcgatg cggatcgaga
    13741 actccttcga ggtcgaggcg cctgccgaag ccgcttggga gctcctgctc gacgtgccga
    13801 gggtcgtcgt ctgcatgccc ggagccgagc tgacgg
//
LOCUS       DBNGNK010000298         4098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1340368,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000298 DBNGNK010000000
VERSION     DBNGNK010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1340368"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="AC150V_08785"
     CDS             <1..472
                     /locus_tag="AC150V_08785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763053.1"
                     /translation="LDRSPEMLAVARTRLDGRAVVIEGRAESLPFADDTFDHLTMTYL
                     LRYVDDPGAVLAELFRVVRPGGTVASLEFGVPRGLWRGAWELYVRIGLPIAGRLISPG
                     WYEVGRFLGSSIKDFYRELPLERQLALWRRAGALDVRFRRLSLGGGVVVWGRRA"
     gene            454..1185
                     /locus_tag="AC150V_08790"
     CDS             454..1185
                     /locus_tag="AC150V_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763054.1"
                     /translation="MGTTRMKPAFYALAPGGWRDYVTLLHLPYTAWHLSYVAVGGCLV
                     ATVSWQRLGWTLLAFALAVGIGAHAVDELAGRPLGTAIPGKVLVGLGAISLAGACAIG
                     LYGAATFNVWLLLFVGAGAFLVPAYGLELLGGRFHTDVVFALGWGAFPVVTGYFAGSG
                     EVRVVTFAAALWAFALSLAQRRLSTPVRRLRRRATGVEGVVEFADGTHEPLTREALVA
                     APEAALRLLTVATVAAAVALVAFRL"
     gene            1209..2120
                     /locus_tag="AC150V_08795"
     CDS             1209..2120
                     /locus_tag="AC150V_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763055.1"
                     /translation="MTVWLLAGLLAVAVVAVVALAFALRQARRRAMINEAAIEAGRAE
                     LTAAVEEATQAHLEEIRRTLARERAETASAVADDERRLAAERRAALLQQERQIADDLA
                     ERLALAEAQVEERLRTFGDDLDRALDQIGGQVVKLQQRQREAIGEVEARIEAEAAELG
                     STADEQRKAVLRLREELEQAANAAVTEALDELADQVAERRRTIDEISERLRAREEAIA
                     EGIERAESDARARLEVTLVEFERRQTERLERQVERDIERHAQAAVVAFDTRMREIRED
                     AAKRLERELERRADLLAREEFAARLEQ"
     gene            2224..2430
                     /locus_tag="AC150V_08800"
     CDS             2224..2430
                     /locus_tag="AC150V_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763056.1"
                     /translation="MAETTQKQTLGHDGEQAKVEGWRLHVLIEAGYPLPLAERLAASD
                     ADLHECVDLIAQGCSPATAAEILL"
     gene            2468..4036
                     /locus_tag="AC150V_08805"
     CDS             2468..4036
                     /locus_tag="AC150V_08805"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR3763057.1"
                     /translation="MNRTEMLERVKARSEPWDLVVVGGGATGVGIAVDAASRGYETLL
                     LEGHDFGKGASSRSTKLAHGGVRYLERGNLSLVVESLRERAIMRRNAPHVVSDLAFVV
                     PSYDWWEAPFYGLGLKVYDLLAGRDGFERSHILSREETLARLPTIRTDGLRGGVVYYD
                     GRFDDARLLVNLAATALEQGATLLNYAHVDAVTLGDDGFVNGVSGRDVDGGGARLEAR
                     ARVVVNATGVFADEVRRLVDPAAADLVAPSQGVHLVLDRSFLPGDTAVLVPHTSDGRV
                     LFAIPWKQRTLVGTTDTPLDEVSLEPRARAEEIDFLLETAACYLTRAPGRGDVKSVFA
                     GVRPLVKGSDKRTTAALSRDHLIHVDDTGLLTITGGKWTTYRKMAEECVDQAAALGGL
                     AELPCTTRELNVHGYHRHPRRFGDLAVYGSDARRLQELARSEPGLGDRLHPDLPALRA
                     EIVWAARAEMALTVEDALARRTRSLFLDAGAAIEAAPATAALLARELGRDEAWQRDQV
                     EAFGALASCYTLSP"
BASE COUNT          605 a         1424 c         1447 g          622 t
ORIGIN      
        1 cctggatcgc agccccgaga tgctcgccgt cgcacgcacg aggctggacg gccgtgccgt
       61 tgtgatcgag ggtagagccg agtcgcttcc gttcgcggac gacaccttcg atcacctcac
      121 catgacctat ctgctgaggt acgtcgacga tcctggcgcc gtcctcgccg agctgttccg
      181 ggtcgtgcga ccgggaggga cggtcgcctc gctcgagttc ggtgtgccgc gcggtctgtg
      241 gcgcggcgca tgggagctct acgtgcggat cggcctgccg atcgccggcc ggctgatttc
      301 tccgggctgg tacgaggtgg gacgctttct cggctcttcg atcaaagact tctatcgcga
      361 gctcccgctc gagcggcaac tcgcgctgtg gcggcgggcc ggcgcactcg acgtccggtt
      421 tcggcggctt agcctcggcg gcggggtcgt tgtgtgggga cgacgcgcat gaagcccgcc
      481 ttctacgcgc tcgctccagg cggctggcgc gactacgtca ccctcctgca tctgccctac
      541 accgcctggc acctctcgta cgtcgccgtc ggcggctgcc tcgtggccac cgtctcgtgg
      601 cagcgactcg ggtggacgct gctggcgttc gcgctcgccg tcggaatcgg cgcccacgcg
      661 gtcgacgagc tcgcgggccg gccgctcggc acggccattc ccggcaaggt gctcgtcggc
      721 ctcggcgcga tctcgctcgc cggggcatgc gcgatcggcc tctacggcgc ggcgaccttc
      781 aacgtctggc tccttctgtt cgtcggcgcg ggcgcgttcc tcgttcccgc ctacgggctc
      841 gagttgctcg gcggccgctt tcacacggac gtcgtgttcg cgctgggctg gggcgccttc
      901 ccggtggtga ccggctactt cgccggcagc ggagaggtgc gtgtcgtgac gttcgcggcc
      961 gcgctctggg cgttcgcgct gtcgctcgcg caacgccgcc tctcgactcc cgtcaggagg
     1021 ttgcggcgac gggcgacggg cgtggagggt gtggtcgaat tcgccgacgg cacacacgag
     1081 cctctcaccc gtgaggctct ggtcgccgct ccggaggcgg cgcttcgcct cttgacggtc
     1141 gccaccgtgg cggcggcggt cgcactcgtc gcgttccgtc tctagcggaa caggggtaga
     1201 ttgagctcgt gactgtatgg cttctcgccg gtctgctggc tgtggcggtg gtcgccgtcg
     1261 tggcactggc gttcgcgctc cggcaagcgc gccggcgcgc catgatcaac gaggcggcga
     1321 tcgaggccgg gcgggccgag ctcactgcgg ccgtcgagga ggcgacgcag gcgcacctgg
     1381 aggagattcg gcgcacgctc gcgcgagagc gggccgagac cgcatcggcg gtcgcggacg
     1441 acgagcgccg actcgcggcg gagcggcgcg ccgcgctgct ccagcaggaa cgacagatcg
     1501 ccgatgacct cgccgaaagg ctggccctcg cggaggccca ggtcgaggag cgcctgcgca
     1561 cgttcgggga cgacctcgat cgcgcgctcg accagatcgg cggccaggtc gtcaagctcc
     1621 agcagcgcca acgagaggcg atcggcgagg tcgaggcgcg catcgaggcc gaggccgccg
     1681 agctcgggtc gacagccgac gagcaacgga aggccgtcct acgccttcgg gaagagctcg
     1741 aacaagcggc gaacgcggct gtcaccgaag cgctcgacga gcttgctgac caggtcgccg
     1801 agcggcggcg cacgatcgac gagatcagcg agcgacttcg cgctcgggag gaagcgatcg
     1861 ccgaggggat cgagcgggcc gagagtgacg ccagagcgcg cctcgaggtg actctcgtcg
     1921 agttcgagcg ccgccagaca gagcgtctgg aacgccaggt cgagcgcgac atcgagcgtc
     1981 acgcgcaggc agccgtcgtg gcattcgaca cgaggatgcg cgagatccgc gaagatgccg
     2041 cgaaaagact ggagcgcgag ctcgagcgcc gtgccgacct gctggcacgt gaagagttcg
     2101 ccgccaggct cgagcagtag ggcggccgtt aatcccaccc ggccgggagg caaggtccac
     2161 ccgcacttca caaacaactc gcggcagggt tcgtcaaaga gaccacgaac atcccccgaa
     2221 ccgatggcag agacgaccca gaaacagacc ctcggacacg acggcgagca ggccaaggtc
     2281 gaaggctggc gtctgcacgt cctcatcgaa gccgggtatc cgctgccgtt ggccgagcgt
     2341 ctggccgcga gcgacgccga tctccacgag tgcgtcgatc tgatcgccca gggctgcagc
     2401 ccggcgacgg cagcagagat cctcctctga tcgcgtcgcc cggcgggcgg ctccgtaaga
     2461 tcggaccgtg aaccgcacgg agatgctcga gcgcgtgaag gcgcgcagcg agccgtggga
     2521 cctggtggtg gtcggcgggg gcgccacggg tgtgggcatc gcggtcgatg cggcttcccg
     2581 cggctacgag acgctcctgc tcgagggaca cgacttcggg aagggcgctt cgagtcgaag
     2641 caccaagctc gcgcacggcg gcgtccgcta cctggaacgc gggaacctgt cgctggtcgt
     2701 cgagtccctg cgcgagcggg cgatcatgcg tcgcaacgcg ccgcacgtcg tctccgatct
     2761 cgccttcgtc gttcccagct acgactggtg ggaggcgccc ttctacgggc tgggattgaa
     2821 ggtctacgac ctgctcgccg gccgggacgg cttcgagcgc tcccacatcc tctcgcgcga
     2881 ggagacgctc gcgcggcttc cgacgatccg caccgacggg ctgcgcggcg gcgtcgtcta
     2941 ctacgacggg cgctttgacg acgcccggct cctcgtcaac ctcgccgcaa ccgcgctcga
     3001 gcagggagcg acgctgctca actacgccca cgtcgacgcg gtaacgctcg gcgacgacgg
     3061 gttcgtgaac ggagtgagcg gccgcgacgt cgacggcggc ggtgctcggc tcgaggcgcg
     3121 ggcacgcgtc gtcgtgaacg ccaccggcgt cttcgccgac gaggtacgtc gcctcgtaga
     3181 cccggccgcg gccgacctcg tcgctcccag ccagggcgtg catctcgtcc tcgaccgctc
     3241 ctttctcccg ggcgacaccg cagtactggt gccgcacacg agcgacgggc gcgtgctgtt
     3301 cgcgattccg tggaagcaga ggacgctggt cggcacgacc gacactccgc tcgacgaggt
     3361 ttcgctcgag ccacgggccc gcgccgagga gatcgacttc ctgctggaga ccgccgcttg
     3421 ctacctgacc cgggcgcccg ggcgtggcga cgtgaagtcc gtcttcgcgg gcgttcggcc
     3481 gctcgtcaag ggctcagaca agcgaacaac agcagcactc tcccgcgacc acctcatcca
     3541 cgtcgacgac accggactcc tgaccatcac gggcgggaaa tggacgacat accggaagat
     3601 ggccgaggag tgtgtcgacc aggcggccgc gctcggcggc ctcgcggaac tcccctgcac
     3661 gacccgtgag ctcaacgtgc acggctacca ccgccacccc aggcgattcg gtgacctcgc
     3721 cgtctacggc tcggatgccc ggcggctgca agagctcgcc cggagcgagc ctggtctggg
     3781 ggaccgtctc caccccgatc tgcccgccct tcgcgcggag atcgtctggg cggcgcgcgc
     3841 cgagatggcg ctcaccgtgg aggacgcgct ggcccggcga acacgctcgc tctttctcga
     3901 cgccggcgcg gccatcgagg ccgcgccggc caccgcggct ctcctggccc gggagctcgg
     3961 gcgtgacgaa gcgtggcagc gcgaccaggt cgaggcgttc ggagcactcg ccagctgcta
     4021 cacgttgtca ccgtgaccac caaggtcgag tcgatccagt acggcgaccg caaggtcttc
     4081 accgtcgcgt ccttcaac
//
LOCUS       DBNGNK010000299         4527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1697586,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000299 DBNGNK010000000
VERSION     DBNGNK010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1697586"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="AC150V_08810"
     CDS             <1..448
                     /locus_tag="AC150V_08810"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR3763058.1"
                     /translation="FDHRPDRAAKKIHAGARFLQLQICYHAARLERFVQALHEAGLTR
                     RAAILPTVVLVRGAHSLRFMNEHVPGIDVPPDTVTHVERADDPSEAAYELALGQARHA
                     LSLPGVRGLHITDFRHDGAVSRLCRDLGIPPKEERGAYAHRSQLAV"
     gene            420..1280
                     /locus_tag="AC150V_08815"
     CDS             420..1280
                     /locus_tag="AC150V_08815"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR3763059.1"
                     /translation="MHTVLSSRSKEVVIGPDQPFCIIGERINPTGRKAFGEALRAGDL
                     SEVVKDVEDQVAMGAHMLDVNMGVPLADEAQLLASAITLVQEHTDLPICIDSSVVEAL
                     EAGLAAYEGKALVNSVTCEEERLERVLPLVKEHGAAVICLTNDETGIPQTAEERLEVC
                     SRLVDIVHGRYEIPLEDIVIDPLAMTVGADPQAVVTTLDTISRIRDRFELNMTLGASN
                     VSFGLPDRHALNAAFLVLASAAGLTSAIMDARTAQTVDACRAGDLLLGRDEWGMNWIA
                     AHRQAQGATA"
     gene            1277..3163
                     /locus_tag="AC150V_08820"
     CDS             1277..3163
                     /locus_tag="AC150V_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR3763060.1"
                     /translation="MTIAMEPPEHTDEPAEQPARVALRFEPSGAEVRVPPGVTVFDAA
                     SWNGIAIDSTCGGHGTCKKCRVRIADDVVPVSKLDARAFEESELDAGWRLACRAQAQH
                     DLLVDVPPLTTRPKAATMGVGRQVILRPALQKRYVELDEPSLEDQRTDFQRLCDGVDD
                     LELHADLDVLRHLPSVLRKSDYRVTCVVSDERLIGVEPGDTTGRCFAIAFDLGTTTVV
                     ATLLDVLTGTPVAVASMLNKQQPFGADVISRISATMLDPEALERLRRHAADTLQALAV
                     EVCEEGSVEPAEVYEVALAGNATMTQLALGIDPEPVGVAPFIMASQSYPDMAAAELGL
                     ELHPRARATVLPALGAYVGGDVVAGLLATGMTRDRRLRLFIDVGTNCEIALGSADEII
                     CTAAPAGPAFEAAQIRCGMRAADGAIEVVRIHDDEVELDVIGGVEPAGICGSGLVDTV
                     SELVRAGLLDASGRFVPDEDAAQTAPRLARRLVTRANGERVFVLHWGGTEGDHDESVY
                     LSQRDVRELQFAKAAIATGWRLLVEELGVDTSEIQQVLLAGSFGSYLSPASAVRIGLV
                     PKLPLPRIVSAGNVAGEGAKMTLLSLPERHAAAAMLDEVRYVELSDRTDFNDRFIEQL
                     AFPS"
     gene            3160..3807
                     /locus_tag="AC150V_08825"
     CDS             3160..3807
                     /locus_tag="AC150V_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR3763061.1"
                     /translation="MTVVLEDRARPRGNVRAKTLVLACGAVAREVLDVVRLNGWGHVD
                     VRCLPAKLHLVPAEIPGAVDRKLAEIAHLYDNVFVAYADCGTAGALDRVLARHGVERL
                     PGAHCYAFYAGRETFEEMHDEEPGTFYLTDFLVRHFDSLVVRALGLDRHPELVGDVFG
                     NYRRLVFLAQTDDSVLLERARAAAERLGLDFEHRRTGYGELQSSLRAAVGVVPVG"
     gene            3800..4111
                     /locus_tag="AC150V_08830"
     CDS             3800..4111
                     /locus_tag="AC150V_08830"
                     /inference="COORDINATES: protein motif:HMM:NF025142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIR3763062.1"
                     /translation="MGELTVISWRSIPAQVTATSGETTERAELSKRFMAAIDAAAMEA
                     GLVGMDEYLGEWRRATRPCGDDVAAEVAAELARLEQAYPQDELRRIVRSGGLDDPPSA
                     A"
     gene            4141..>4527
                     /locus_tag="AC150V_08835"
     CDS             4141..>4527
                     /locus_tag="AC150V_08835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3763063.1"
                     /translation="MPWDALSEEQQEIRELVRALARDRIAPRAAEIDESHEFPWDIVE
                     LFREHELFGLFFAEEWGGTGTGTLLSLVAIEEVSKVCATSGVILAVQELGSLGLKLGA
                     TREQQERYLPLLASGEWLTAYALTEAG"
BASE COUNT          666 a         1574 c         1573 g          714 t
ORIGIN      
        1 gttcgatcat cggccggacc gcgctgcgaa gaagatccac gcaggcgccc gcttcctcca
       61 gctgcagatc tgctaccacg cggctcggct cgagcgcttc gtccaggccc tgcacgaggc
      121 gggactcacc cgccgcgcgg cgatcctgcc gaccgtcgtg ctcgtccgtg gcgcacacag
      181 cctccgcttc atgaacgagc acgtccctgg catcgacgtt ccgcccgaca cggtcacgca
      241 cgtcgagcgg gcagacgatc cgtccgaggc cgcctacgag cttgcgctcg gtcaggcacg
      301 ccacgcgctg tcgctaccgg gcgtccgggg gctgcacatc accgatttcc gtcatgacgg
      361 tgccgtctcg aggctctgcc gcgatctcgg cattccgccc aaggaagaga gaggagcgta
      421 tgcacaccgt tctcagctcg cggtctaagg aggtcgtgat cggccccgac cagccgttct
      481 gcatcatcgg cgagcgcatc aacccgaccg gccgcaaggc gttcggagag gcgctccggg
      541 caggcgatct gtccgaggtc gtcaaagacg tggaggacca ggtcgctatg ggtgcccaca
      601 tgctcgacgt caacatgggg gtgccgctcg cagacgaggc ccagctgctc gcgagcgcga
      661 tcacgctggt gcaggagcac acggacctcc cgatctgtat cgattcttcc gtcgtggagg
      721 cgctcgaggc cggcctcgcc gcgtacgagg gcaaggcgct cgtcaactcg gtcacctgcg
      781 aggaggagcg actcgagcgc gtgctgccgc tcgtcaagga gcacggcgcg gccgtgatct
      841 gcctgaccaa cgacgagacg ggcattccgc agacggccga ggagcggctc gaggtctgca
      901 gccgcctcgt cgacatcgtg cacggtcgct acgagatccc gctcgaggac atcgtgatcg
      961 atccgctggc catgacagtc ggcgccgatc cgcaggcggt cgtgacgacg ctcgacacga
     1021 tcagtcgcat ccgcgaccgg ttcgagctca acatgacgct cggcgcgtcc aacgtctcgt
     1081 tcggtctgcc cgaccggcac gcgctcaatg ccgcgtttct cgtgcttgcg tccgccgctg
     1141 gcctgacgtc ggcgatcatg gacgcccgca cagcccagac cgtcgatgcc tgccgcgccg
     1201 gcgacctcct gctcgggcgc gacgagtggg gcatgaactg gatcgctgct caccgacagg
     1261 cgcagggggc cactgcgtga cgattgccat ggagcctccg gagcacaccg acgagccggc
     1321 cgagcagccg gcgcgcgtcg ctctgcgctt cgagccctcc ggcgcggagg tccgggttcc
     1381 gccgggcgtg accgtcttcg acgccgcctc ctggaacggg atcgcaatcg actccacgtg
     1441 cggcggccac ggcacctgca agaaatgtcg cgtccggatc gccgacgacg tcgtgcccgt
     1501 gagcaagctg gacgctcgcg ccttcgagga gtcggagctc gacgcggggt ggcgcctggc
     1561 gtgtcgcgcg caggcgcagc acgacctcct cgtcgacgtt ccgccgctca ccacccgacc
     1621 gaaggccgcg acgatgggcg tcggccgcca ggtgatcctc cggcctgcgc tccagaagcg
     1681 ctacgtcgag ctggacgagc cgtcgctcga ggatcagcgc accgacttcc agcgcctctg
     1741 cgacggggtc gacgatctcg agcttcacgc cgatctcgac gtgctgcgcc acctgccgag
     1801 cgtgctgcga aagtcggact accgggtgac ctgcgtcgtc tccgacgagc ggctcatcgg
     1861 tgtcgagccg ggtgacacga ccggtcgctg tttcgcgatc gcgttcgatc tcgggacgac
     1921 gaccgtcgtc gcgacgctgc tggacgtcct cacggggacg ccggtcgccg tcgcctccat
     1981 gctgaacaag cagcagccgt tcggagccga cgtcatttcg cgcatctccg ccacgatgct
     2041 cgacccggaa gcgctcgagc ggctgcggcg tcacgcggcc gacacgctgc aggccctcgc
     2101 cgtggaggtc tgcgaagagg gttctgtgga gccggcggaa gtgtacgagg tcgcgctggc
     2161 cggcaacgcg acgatgaccc agctggcgct cggcatcgac ccggagcccg tcggtgtcgc
     2221 ccccttcatc atggcttcgc agtcctaccc cgacatggcc gcggcagagc tcggcctcga
     2281 gctccacccg cgagcccgcg caaccgtcct gccggccctg ggggcctacg tcggcggtga
     2341 cgtcgtcgcc gggctgctcg cgaccggtat gacgcgcgat cgccggctgc gattgttcat
     2401 cgacgtcggc acgaactgcg agatcgcgct cggctccgcc gacgagatca tctgcaccgc
     2461 tgcgccggcc gggccggcct tcgaggcggc gcagatccgt tgcgggatgc gcgccgccga
     2521 cggtgcgatc gaagtcgtgc ggatccacga cgacgaggtc gagctcgacg tgatcggtgg
     2581 cgtcgagccc gcgggcatct gcgggtcggg cctcgtcgac acggtgtcgg agcttgtccg
     2641 cgcgggtctg ctcgacgcga gcgggcgctt cgtgccggac gaggacgcgg cgcagacggc
     2701 ccctcgactc gcccgcaggc tcgtcacccg cgccaacggc gagcgggtct tcgtgctgca
     2761 ttggggcggt acggagggtg accacgacga gtccgtctac ctgtcgcagc gcgacgtccg
     2821 cgagctgcag ttcgccaagg cggcgatcgc gaccggctgg cggctgctcg tcgaagagct
     2881 cggagtcgac acgtccgaga tccagcaggt gctgctggcg ggctccttcg gttcctacct
     2941 ctcgcctgcg agcgccgttc gcatcgggct cgtgccgaag ctgccgctgc cgcggatcgt
     3001 gtcggcgggc aacgtcgcgg gggagggggc gaagatgacg ttgctctcgc tccccgagcg
     3061 tcacgccgcg gcggcgatgc tcgacgaagt ccgctacgtg gagctctccg accgcacgga
     3121 tttcaacgat cgcttcatcg agcagctcgc gtttccgtcg tgaccgtggt gctggaagac
     3181 cgagcccgac cgcggggcaa tgtgcgagcg aaaacccttg tgctcgcctg cggcgccgtt
     3241 gcgcgcgagg tgttggacgt cgtccgcctg aacggatggg ggcacgtcga cgtgcgctgc
     3301 ctgccggcca agctccacct cgtgccggcg gagattcccg gtgcggtcga ccggaagctg
     3361 gccgagatcg cgcacctcta cgacaacgtg ttcgtcgcct acgcggactg cgggacggcg
     3421 ggcgccctcg accgcgttct cgcacgccac ggcgtcgagc gcctgccggg cgcccactgt
     3481 tacgcgttct acgccgggcg ggaaaccttc gaggagatgc acgacgaaga gcccggaacc
     3541 ttctacctga ccgatttcct cgtccggcac ttcgactcgc tcgtcgttcg cgctctaggg
     3601 ctcgaccggc atccggagct cgtcggcgac gtgttcggca actaccggcg ccttgtgttc
     3661 ctggcgcaga ccgacgacag cgtgctgctc gagcgcgcgc gagccgcagc tgaacggctc
     3721 gggctcgact tcgagcaccg gcgaacaggg tacggcgagc tccagtcctc gctccgcgct
     3781 gcagtgggag tcgtgccggt tgggtgagct gaccgtcatc tcctggcgga gcattcccgc
     3841 gcaggtgacg gccaccagcg gtgagacgac ggaacgagca gagctctcga agcgcttcat
     3901 ggccgcaatc gatgcggcag caatggaggc cgggctcgtg ggcatggacg agtatctcgg
     3961 cgagtggcgc cgcgccacgc ggccctgcgg agacgatgtg gcggccgagg tggccgccga
     4021 gctggcacgg ctcgagcagg cctacccgca ggacgagctg cggcgcatcg tccgctcagg
     4081 cggactggac gacccgccgt ctgccgcctg accgcccagc tcggcgccta cactttcgct
     4141 gtgccgtggg acgccctctc cgaggaacag caggagatcc gtgagctcgt gcgggcgctc
     4201 gcgcgcgacc ggatcgcgcc gagggccgcg gagatcgacg agtcgcacga gttcccctgg
     4261 gacatcgtcg agctcttccg tgagcacgag ttgttcgggc tcttcttcgc ggaggagtgg
     4321 ggcggaaccg gtaccggcac cctgttgtcg ctcgtggcca tcgaggaggt ttcgaaggtc
     4381 tgcgcgacga gtggcgtgat cctcgccgtg caggagctcg gctcgctcgg cctcaagttg
     4441 ggggccacgc gagagcagca ggagcgctac ctgccgctgc tcgcctcggg cgaatggctg
     4501 acggcgtacg ccctgaccga ggccggc
//
LOCUS       DBNGNK010000300         1730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_196575,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000300 DBNGNK010000000
VERSION     DBNGNK010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_196575"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..689)
                     /locus_tag="AC150V_08840"
     CDS             complement(75..689)
                     /locus_tag="AC150V_08840"
                     /inference="COORDINATES: protein motif:HMM:NF028235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3763064.1"
                     /translation="MGRTSDARERLIDAAFGLWFTRSYADVGVSEICEEAGVQKGSFY
                     HFFPSKTDLALAVIDDIWGRYRTDVAEPVLEDASTPPLDRLEILIERNYQFAVANKAA
                     TGHVWGCPVGNLAVELSTQEEAVRERLSELFGQWAGEFTQLLDEAVAAGDLPPRDTRV
                     AGLSLLAYVQGMTVLAKTGNDPDLIRTIGPTIVAVASAPAGVAA"
     gene            complement(756..>1730)
                     /locus_tag="AC150V_08845"
     CDS             complement(756..>1730)
                     /locus_tag="AC150V_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR3763065.1"
                     /translation="YLARTAVEMPSSHAVARGLGEVVVGEDPLAIDRLWQRMYEASSY
                     YGRAGVALHAISAIDMALWDLAGKAEGRPVSALLGGANAARLLVYASEVMPAGVEEVA
                     RIAHAAVEGGYGALKLGWGPLGTDADLDEALIRSARESLGPARLLMIDGGQAYTVESA
                     GELLRRVEDVELYWFEEPFWPDDYESYRRLADVAPVRLAAGEADSTIVPFRRLVEEGG
                     VNVLQPDLGRCGGFSVARRIAELAGARGVEVVPHCFSTGLLVAASLQFACTLDTPTLS
                     EYSVADSPLAGGLLAEPFVLEEGALAVPAGPGLGVELDEELVGTLRLG"
BASE COUNT          281 a          637 c          566 g          246 t
ORIGIN      
        1 tgttgccgca cggagcggct gttagttgac cagtcgtcta ttagaggtga aaaagaagag
       61 ggggtgcgcg cggctcatgc cgccaccccc gccggcgcgc tcgcaacggc gacgatcgtc
      121 ggcccgatcg tgcggatcaa atccgggtcg ttccccgtct tcgcgagcac ggtcatgccc
      181 tgcacgtagg cgaggagcga gagcccggcc acgcgcgtgt cgcgcggcgg caggtcgccg
      241 gcggcgacgg cctcgtccag cagctgcgtg aactcgccgg cccactggcc gaagagctcg
      301 ctcaaccgct cacggaccgc ttcctcctgc gtcgaaagct cgacggcgag gttcccgacg
      361 gggcatcccc acacgtgccc cgtcgccgcc ttgttggcga cggcgaactg gtagttccgc
      421 tcgatcagga tctccaaccg gtcgagcgga ggcgtgctcg cgtcctccag gaccggctcg
      481 gccacgtccg tccggtaccg cccccagatg tcgtcgatga ccgcgagggc gaggtcggtc
      541 ttggatggga agaagtggta gaaactcccc ttctgaacgc cggcctcctc gcagatctcg
      601 ctgacgccga cgtctgcgta gctccgtgtg aaccagagcc cgaaggcggc gtcgatcaac
      661 cgttcgcgtg cgtcacttgt ccgtcccata gttgactggt cggtacagta acacggtacg
      721 cccgccctcg tcaaggacgc cgccgagcgg cagactcagc cgagccgcag cgtgccgacg
      781 agctcctcgt cgagctcgac gccgagccca gggccggccg gcacggccag cgcgccttcc
      841 tctagcacga acggctcggc gaggagtccc cctgcgagcg gggagtccgc caccgagtac
      901 tcggacagcg tcggcgtgtc gagcgtgcag gcgaactgga gcgaggcggc gacgagcagc
      961 cccgtcgaga agcagtgcgg gacgacctcc acgcctcgcg cgccggccag ctcggcgatc
     1021 cgccgggcga cactgaaccc gccgcagcga ccgaggtcgg gctggagcac gttcacgcct
     1081 ccctcctcga cgagtcgccg gaaaggcacg atcgtcgagt cggcctcacc cgcggcgagc
     1141 cgcacgggag cgacgtctgc gagccgccgg tagctctcgt agtcgtcggg ccagaacggc
     1201 tcctcgaacc agtagagctc gacgtcctcg acacgccgaa gcagctcgcc cgcactctcg
     1261 accgtgtagg cctgaccccc atcgatcatc aacaatctcg ccggcccgag cgactcccgg
     1321 gcggagcgga tcagcgcctc gtcgagatcg gcatcggtgc cgagcgggcc ccagccgagc
     1381 ttgagcgccc cgtagccgcc ttcgacggcg gcatgcgcga tgcgcgcgac ctcctcgacc
     1441 cccgccggca tcacctcgct cgcatacaca agtagtcgcg ccgcgtttgc cccgccgagc
     1501 agcgcggaga ccggacgtcc ctctgctttc ccggcgagat cccacagagc catgtcgatc
     1561 gcgctgatcg cgtgcagggc gacgccggcg cgtccgtagt acgacgaggc ctcgtacatt
     1621 cgctgccaca ggcggtcgat cgcgagcggg tcttcaccca cgaccacctc cccgagccca
     1681 cgcgcaaccg cgtgcgacga cggcatctcc accgccgtgc gggcaaggta
//
LOCUS       DBNGNK010000301         5526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1484385,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000301 DBNGNK010000000
VERSION     DBNGNK010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1484385"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC150V_08850"
     CDS             <1..399
                     /locus_tag="AC150V_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763066.1"
                     /translation="QTREQHIRREKATSNITTNQTLLALGGLVYLSWLGPQGLRDVGE
                     ACVALGEYAKERIGLEPAFDRATFKEVAFRLPRPARDVIRAARERGVHPGYALGRDYA
                     GMDDVLLVAVTERRTAAEIDRLADVLAEVC"
     gene            399..1865
                     /gene="gcvPB"
                     /locus_tag="AC150V_08855"
     CDS             399..1865
                     /gene="gcvPB"
                     /locus_tag="AC150V_08855"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR3763067.1"
                     /translation="MQLLFERSRRGRRAGTVPEHGLPVPELPQELARSRPPRLPEISE
                     SELVRHFTALSERNFGIDTGFYPLGSCTMKHNPRVNERVVALPGFRDLHPHQEDEGAQ
                     GALELMWRLQATLTEIAGLPGLTLQPAAGSQGELTGLMLMRAYFADRGEGDRRDTVIT
                     ADTAHGTNPASVTMAGYQLEKVATDERGNIDLDDLRGKVSERTAGLMLTNPSTLGLFD
                     EGIEEIADIFHSQGALLYYDGANLNAIVGRSRPGDMGFDIVHYNLHKTFSQPHGGGGP
                     GAGPLAVRDMLEPYLPAPVVVRDGDVFRLDHDRPKSIGRVRGFAGPFGVFVRAYVFMR
                     AYGPSLREMSDDAVLNANYLLARLRDAYELPYDRHCMHEFVLSARQLKRAHGVTALDV
                     AKRLMDFGIHPPTIYFPLVVPEALMIEPTETEPKERLDAFVEAMQQIAREAALEPDKL
                     HAAPTTRSVRRLDEVGAARHPVVKYDFDEASDRVEAPS"
     gene            1943..2320
                     /locus_tag="AC150V_08860"
     CDS             1943..2320
                     /locus_tag="AC150V_08860"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3763068.1"
                     /translation="MNAPTLAVAHELVELSHELAQAVVRSDRDRLDSLLAEEFTLNGA
                     AGVLDRESFLEAAAGEYDIDEFAYEEIDPEVYGDTGVCVSRYRQEASFDGRDLSGTMH
                     VTDIWVRRDGRWQIVRRHASPTD"
     gene            complement(2322..2888)
                     /locus_tag="AC150V_08865"
     CDS             complement(2322..2888)
                     /locus_tag="AC150V_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020737915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR3763069.1"
                     /translation="MAKLFETLEPKLVDWIERQRMFFVGTAPANGGHVNVSPKGPIGA
                     LRILDETTVAYLDWIGSGVETISHLRENGRIVVMLCAFEGPPRIVRLHGHGTVHEPDS
                     ARFAELAPTFGLDDRADAHRSVIEVELERISDSCGYGVPLMEYQGERSQMLDWIGSQL
                     RKSPEALDEYARENNFASIDGLQGLTKR"
     gene            complement(2898..3896)
                     /gene="rlmN"
                     /locus_tag="AC150V_08870"
     CDS             complement(2898..3896)
                     /gene="rlmN"
                     /locus_tag="AC150V_08870"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR3763070.1"
                     /translation="MNLELLETTLAQRGEPGYRAHQVWEWTARGAAGYEEMTNLPAAL
                     RDDLSAAVQFSTLTLDTEAHSADGTRKALFRTTDGHSVEAVLMRYRDGRRSVCLSSQS
                     GCPLTCTFCATGAMSFGRNLSASEILDQVLHFRRVEPVDHVVFMGMGEPMMNLDDVLE
                     AARRLPAVGVTHRRTTISTVGWMPGLRRFVADVAEPIRLALSLHAADPELRRTLMPVE
                     ERYPLEEVLAECRRYVELRRRKVFVEYVMLAGVNDSPRHARELAALLDRSLFKVNLIP
                     YNPTGRFEGSSRDAIAAFKAVLDRARLPSTVRLTRGRDIAAACGQLAAEPRVAAAR"
     gene            3923..4123
                     /locus_tag="AC150V_08875"
     CDS             3923..4123
                     /locus_tag="AC150V_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763071.1"
                     /translation="MTEPTYVSRVRIERIRGPLRRADLPGDEEPTYFGVHDEVADHYG
                     VPRGSEAERATTLDHVVAAAAG"
     gene            4145..4411
                     /locus_tag="AC150V_08880"
                     /pseudo
     CDS             4145..4411
                     /locus_tag="AC150V_08880"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
     gene            4509..5171
                     /locus_tag="AC150V_08885"
     CDS             4509..5171
                     /locus_tag="AC150V_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR3763072.1"
                     /translation="MNSSTAQRTYPVTVEGELEEPLSRWLWLVKWLLLIPHFLILIVL
                     WIAFFVLTVIAFFAILFTGRYPRGIFNFNVGVLRWTWRVSFYGYSALGTDRYPPFMLD
                     DVPDYPARLEVAYPERLSRGLVLVKWWLLAIPHYLVIGTFQGGWGATHGLVGLLVLFV
                     AVALLFTTRYLSGVFPFLMGMNRWTFRVVAYAALMRDEYPPFRLDMGAKEPAGTLTAQ
                     SH"
     gene            5315..>5526
                     /locus_tag="AC150V_08890"
     CDS             5315..>5526
                     /locus_tag="AC150V_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HIR3763073.1"
                     /translation="MIDLRSDTATRPSPEMRAAIAAAQVGDEQKREDPSVTALQDRVA
                     ALLGQERAIFLPTATMANHIALKLHSR"
BASE COUNT          849 a         1897 c         1916 g          864 t
ORIGIN      
        1 cagacccgcg agcagcacat ccgtcgtgag aaggcaacct cgaacatcac gacgaaccag
       61 acgctgctcg cgctgggcgg gctcgtctac ctgagttggc tcggcccgca ggggctccgc
      121 gacgtgggcg aggcctgcgt cgcgctgggc gaatacgcga aggagcgcat tggtctggag
      181 ccggccttcg accgagcgac gttcaaggag gtggcgttcc ggctgccgcg gccggcccga
      241 gacgtgatcc gggccgcccg cgaacggggc gtgcatccgg gctacgcact cggacgggac
      301 tacgcgggca tggacgacgt cttgctcgtc gccgtgacgg agcggcgcac cgccgccgag
      361 atcgatcgtc tggcggacgt gctcgcggag gtctgctgat gcagcttctc ttcgagcgct
      421 cgcgccgcgg tcggcgcgcc gggacggtgc cggagcacgg gctgcccgtg cccgagctgc
      481 cacaggagct tgcgcggagc cggccccccc gcctgcccga gatctcggag tccgagctcg
      541 tccgccactt cacggctctg agcgagcgga acttcgggat cgacacgggc ttctacccgc
      601 tcggctcctg caccatgaag cacaacccgc gcgtgaacga gcgtgtcgtc gcgctgccgg
      661 ggttcaggga cctccatcct caccaggagg acgagggcgc gcagggtgcg ttggagctga
      721 tgtggcgcct gcaggcgacc ctgacggaga tcgccgggct gcccggccta acactccagc
      781 ctgcggcggg ctcgcagggc gagctgaccg ggctgatgtt gatgcgcgcc tactttgcgg
      841 accgcggcga gggcgaccgg cgcgacacgg tcatcacggc cgacacggcg cacggcacca
      901 accccgcgag cgtcacgatg gctggctacc agctcgagaa agtcgccacc gacgagcggg
      961 ggaacatcga cctcgacgac ctgcgtggga aggtgagcga gcggacggcg gggttgatgc
     1021 tgacgaaccc ctcgacgctg ggactcttcg acgaggggat cgaggagatc gccgacatct
     1081 tccactccca gggcgcgctc ctctactacg acggcgccaa cctgaacgcg atcgtcggtc
     1141 gctcccggcc cggcgacatg ggcttcgaca tcgtccacta caacctgcac aagacgttct
     1201 cgcagccgca cggcggcggt gggccgggcg ccggcccgct cgcggtgcga gacatgctcg
     1261 agccgtatct gccggcaccc gtagtcgtcc gtgacggcga cgtgttccgg ctcgaccacg
     1321 accgaccgaa gtcgatcggg agggtgcgcg gcttcgcagg ccccttcggc gtcttcgtcc
     1381 gtgcctacgt cttcatgcga gcgtacggcc ccagcctgcg cgagatgtcc gacgacgcgg
     1441 tgctgaacgc caactacctg ctggcccggc tacgggacgc gtacgagctc ccgtacgacc
     1501 ggcattgcat gcacgagttc gtcctctccg ccaggcagct gaagcgggcc cacggcgtca
     1561 cggcgctcga cgtggccaag cgcctgatgg acttcggcat ccatccgccc acgatctact
     1621 tcccgctcgt ggtgccggag gcgctgatga tcgagccgac cgagaccgag ccgaaggagc
     1681 ggctggacgc gttcgtcgaa gccatgcagc agatcgcgcg cgaagccgcc ttggagcccg
     1741 acaagctgca tgccgctccc accacgcgtt cggtgcgacg gctcgacgag gtcggtgccg
     1801 cgaggcatcc ggtcgtcaag tacgacttcg acgaggcctc agaccgggtc gaggcgccga
     1861 gctgagtgcg gccgtcatcc cgctcttgta cgatcgcctc tcgtgcgagc gctcgtggcc
     1921 gacaaggtga agacgcgctg acatgaacgc tcctacgctc gccgtcgccc acgaactcgt
     1981 cgagctgtcc cacgagctcg cgcaggccgt cgtccggagc gaccgagatc gcctggactc
     2041 cctgctggcc gaggaattca cgctcaacgg tgcagccggc gtcctcgacc gggagtcctt
     2101 cctcgaggcc gccgccggcg agtacgacat cgacgagttc gcctacgagg agatcgatcc
     2161 cgaggtctac ggggacactg gcgtgtgcgt ttctcgctac cggcaggagg cgagcttcga
     2221 cggccgcgac ctgagcggga cgatgcacgt caccgacatc tgggtgcggc gcgacggccg
     2281 ctggcagatc gtgcggcggc acgcgagccc cacagactga ctcagcgctt cgtcagcccc
     2341 tgcaggccgt cgatgctggc gaagttgttc tcccgcgcgt actcgtcgag cgcctcgggg
     2401 ctcttgcgca actggctgcc gatccagtcg agcatctgcg accgctcgcc ctggtactcc
     2461 atcagcggca cgccgtagcc gcacgagtcg ctgatccgct cgagctcgac ctcgatcacc
     2521 gaccggtgcg cgtcggctcg gtcgtccagg ccgaatgtcg gcgccagctc ggcgaaccgg
     2581 gcagagtcgg gctcgtggac ggttccgtgc ccgtggaggc ggacgattcg cggtgggccc
     2641 tcgaaggcac agagcatgac gacgatgcgc ccgttctcgc gcagatggga gatcgtctcg
     2701 acgccgctgc cgatccagtc gaggtaggcg accgttgtct cgtcgaggat ccgcagggcg
     2761 ccgatggggc ccttcggcga cacgttcacg tggccgccgt tcgccggggc cgttccgacg
     2821 aagaacatcc gctgccgctc gatccagtcg acgagcttcg gctcgagcgt ctcgaagagc
     2881 ttggccatcg cgacaggcta gcgggctgcg gcgacccgcg gctcggctgc gagctgcccg
     2941 caggcggcgg cgatgtcacg gccgcgggtg agccgcacgg tcgacgggag tcgggcgcga
     3001 tcgagaaccg ccttgaacgc tgcaatcgcg tcgcgcgagg agccttcgaa gcggccggtc
     3061 gggttgtagg ggatcaggtt gaccttgaac agcgagcggt cgaggagggc tgcgagctcg
     3121 cgcgcgtgcc gcgggctgtc gttgacgccg gccagcatca cgtactcgac gaagaccttg
     3181 cggcggcgca gctcgacgta gcgcctgcac tcggccagca cctcctcgag cgggtagcgc
     3241 tcctccaccg gcatcagcgt ccgccgcagc tccgggtcag ccgcgtggag cgagagagcg
     3301 agccggatcg gctcggccac gtcggcgacg aagcggcgca ggcccggcat ccacccgacc
     3361 gtcgagatgg tcgtgcgtcg gtgggtcacg ccgacggccg gcagccgccg cgcggcttcg
     3421 agcacgtcgt cgaggttcat catcggctcg cccatgccca tgaagacgac gtggtcgacg
     3481 ggctcgaccc ggcggaagtg gagaacctgg tcgaggattt ccgaggccga gagattgcgg
     3541 ccgaagctca tggcgccggt cgcgcagaac gtgcacgtga gggggcagcc cgactgggac
     3601 gagaggcaga ccgagcgcct tccgtcgcgg tagcgcatca gcaccgcctc gaccgagtgt
     3661 ccgtcagtcg tccggaacag cgccttgcgg gtgccgtcgg cggagtgggc ctcggtgtcg
     3721 agcgtgagcg tcgagaattg cacggcggcc gacagatcgt cacggagcgc tgccggcagg
     3781 ttcgtcatct cctcgtaccc ggcggctccg cgcgcggtcc actcccagac ctggtgtgcg
     3841 cggtaaccgg gctcgccgcg ctgggcgaga gtcgtctcca gcaattcgag gttcacgaca
     3901 acgagggtag tacgatcgtg tcgtgacaga gccgacatac gtgtcgcgcg tcaggatcga
     3961 gcgcatccgc ggtcccctgc gtcgcgccga tcttcccggc gacgaagagc ccacctattt
     4021 cggcgtccac gacgaggtcg ccgatcacta cggcgttccg cgggggagcg aagccgagcg
     4081 cgcaaccacg ctcgaccacg ttgtcgcggc cgctgcgggc tgactcaccg gcaccttcgg
     4141 gggcgcgctg gaagcgcgcc gcgtcgatgc gagcgagggg cggctgacgt cggaagcgga
     4201 gggcgacgtc gaggccgagg acggcgtgct ggtgatcaag cgcatccgcg tgcgctaccg
     4261 gctcgtggtc gacgacgaca cggacttgga agcggtcggg cgcgcattcg cccatcacat
     4321 gccgaagtgt ccggtctacc gttcgctcag ggacgcgatc gagatggaga ccgacctcga
     4381 gatcgtcgcc gagtcgcccg aaggcgcgta gagcccgcga taaggggtcg gtcgtcgggt
     4441 cgccgtgacc cgcgctgacg gttctcgctt gacagccctc ggtggtcacg aaaggctcag
     4501 taagccgcat gaactcctcg accgcgcagc gcacctatcc ggtcaccgtc gagggcgagc
     4561 tcgaggagcc gctctcgcgc tggctgtggc tggtcaaatg gctgctcctg atcccgcact
     4621 tcctgatcct gatcgtcctc tggatcgcgt tcttcgtgct cacggtcatc gcgttcttcg
     4681 ccatcctctt cacagggcgc tacccgcgcg ggatcttcaa cttcaacgtc ggcgtgctgc
     4741 ggtggacctg gagggtctcg ttctacggct acagcgcgct cggcaccgac cgctacccgc
     4801 cgttcatgct cgacgacgtg cccgactacc cggcacgact cgaggtcgcc tacccggagc
     4861 ggctctcgcg cgggctcgtc ctcgtcaagt ggtggctgct ggcgattccc cactacctgg
     4921 tgatcggtac cttccagggc ggctggggcg cgacgcacgg tctcgtcgga ctcctcgttc
     4981 tcttcgtcgc cgtcgcgctt ctcttcacga cgcggtatct cagcggcgtg ttccccttcc
     5041 tcatgggaat gaaccgctgg acgttccgtg tcgtcgccta cgcggccctg atgcgcgacg
     5101 agtacccgcc cttcaggctc gacatggggg caaaggagcc ggcgggcaca ctcacggcgc
     5161 aatcgcactg agcccgggcc gacggcgtag ctcgaccggt agccggcagc atttcgcacg
     5221 cgtccgctcc tgatcgctcc ggaatcctta cgggcctctc gctggcgcgc gccttggatg
     5281 ccgctggtac gcggactctg gttgaatcgc gcgagtgatc gacctccgct ccgacactgc
     5341 gacgcggccg agccccgaga tgcgcgcggc gattgcggcc gcccaggtgg gggacgagca
     5401 gaagcgcgag gacccgagcg tgacggcgct gcaggaccgg gtcgcggcgc tactcggcca
     5461 ggagcgggcg atcttcctcc cgacggccac gatggccaac cacatcgcgc tgaagctgca
     5521 ctcccg
//
LOCUS       DBNGNK010000302         3266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_624909,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000302 DBNGNK010000000
VERSION     DBNGNK010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_624909"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="AC150V_08895"
     CDS             complement(<1..179)
                     /locus_tag="AC150V_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR3763074.1"
                     /translation="MSGDAQRTIAVIGAALDLGQRRRGVDMGPSAIRYAGLDERLRSI
                     GLDVRDVGNVETAVQ"
     gene            417..489
                     /locus_tag="AC150V_08900"
     tRNA            417..489
                     /locus_tag="AC150V_08900"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:450..452,aa:Phe,seq:gaa)
     gene            493..1887
                     /locus_tag="AC150V_08905"
     CDS             493..1887
                     /locus_tag="AC150V_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR3763075.1"
                     /translation="MSLELAFLPATEQARLVRERQAAPVELVAAALERIERLEPQLNA
                     FVTLRAEEALVEARAAEQAPEAPFRGIPIGIKDLTATAGIRTTYSSRAFADNVPDFDA
                     VVVRRIRDAGFVVVGKTNTPEFGTTPFTESELNGACRNPWCLDLTPGGSSGGAAAAVA
                     AGLLPVAHGSDGGGSIRIPASCCGLFGLKPARGRVSSAPFPSLEGLGTSGPIARTVAD
                     AAALLDVLSGYEPGDPWWAPPPERPFAEEAELEPEPLRVAVTTQPPLDVAVEPECSAT
                     AGEAAELLAGLGHDVTEATPEWTTDLETFLVVWQLGPALFGVRDLELLTPINRMLAER
                     AAATSAVEYGYAVARLHTLARRVVGFWDRYDILVTPALALPPVPIGWQEEEPDPLEQL
                     FRGATFTPFTAIANLTGLPAASIPFAWNRAGLPLGVQLIGPPAGEALLLRVSAQLERA
                     RPWLDRRPPELDRA"
     gene            complement(1944..2762)
                     /locus_tag="AC150V_08910"
     CDS             complement(1944..2762)
                     /locus_tag="AC150V_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR3763076.1"
                     /translation="MFITFLTDFGLGDDFVGTCHGVIKRIAPEAQIIDITHGIPPQAV
                     LQGALMLANTIPYMPVGVHLAVVDPGVGGARRALALTDGEGRIFVGPDNGLLLPAADR
                     AGIVEARELTNERYWLDTISRTFHGRDIFAPVAAHLVRGVEPAQLGPPLAVDALVRLD
                     VPEPSFGDGSVEATMLYADSFGNIALNLTRDDVQQIGVVPGTRVELDLSGERYYAVSA
                     RTFADARPGDVILYEDSYRNMSVAISNGSAAAMLHPQPGRRIRIRTLGLGGRMR"
     gene            complement(2772..>3266)
                     /locus_tag="AC150V_08915"
     CDS             complement(2772..>3266)
                     /locus_tag="AC150V_08915"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR3763077.1"
                     /translation="YDWVVLTSAHGARELRRRMRGTPRRVAAIGSATAAAFGGADLVA
                     TVSTQEGLLEELPRPAGRVLFAGAEGARRLLAEELRADFVPLYRTAELRPDPAPEGDL
                     AVVASPSAARALASLPLRPPVVSIGPQTTLAARAAGLEVLAEAETQDVAGLLAAVERT
                     AEAS"
BASE COUNT          477 a         1187 c         1152 g          450 t
ORIGIN      
        1 tcctggacgg cagtctcgac gttgccgacg tcacgcacgt cgaggccgat ggaccgcagc
       61 cgctcgtcga gccccgcata gcggatagcc gacgggccca tgtcgactcc gcggcgtcgc
      121 tggccgagat ccagcgcagc gccgatgacc gcgatggtgc gctgagcgtc gccgctcacg
      181 ggccgaccct acccgagcgt gccggacatg ccccgcctct gaggaccgca cgagccgact
      241 cggacgcgtc cgtacgggac ggcgagccgc tcgcacgtgc gccgggcggc gaacagcggc
      301 cgcgtcccac cgagtcagac ggacctcaga ggtggggcac ggctcgaagg gccacctcca
      361 tcccggtggt cgacgccgat tcccggcgga accgcgccgc tacactgcga gaccgcggcg
      421 aggtagctca gctggtagag cacacggctg aaaaccgtgg tgtcgccggt tcaagtccgg
      481 ccctcgccat tcgtgagcct cgagctcgcc ttcctgccag caaccgagca ggcccggctc
      541 gtccgggagc ggcaggcagc gccggtcgag ctcgtcgcgg cggcgctcga gcgcatcgag
      601 cggctcgaac cgcagctgaa cgcgttcgtc accctgcggg ccgaggaggc tctcgtggaa
      661 gcccgggcgg cagagcaggc gccggaggcg ccgttccgag gcatcccgat cgggatcaag
      721 gacctgacgg ccaccgccgg gatccgcacc acctattcgt cgagagcgtt tgcggacaac
      781 gtgccggact tcgacgccgt cgtcgtccga agaatccgcg acgccggctt cgtcgtcgtc
      841 ggcaagacca acacgcccga gttcggcacg acgcccttca cagagtcaga gctgaacggc
      901 gcctgtcgca acccgtggtg cctcgacctc acacccggcg gatcgagcgg cggagcagcc
      961 gctgccgtcg cggccggcct gcttccggtc gcgcacggca gcgacggcgg cggctcgatc
     1021 cggatacccg cctcctgctg cggcctgttc ggcctgaaac ccgctcgcgg gcgggtgtca
     1081 tcggcgccgt tcccctccct cgagggtctc ggcacatcgg ggcccatcgc gcggacggtg
     1141 gcagatgcgg cggccctgct cgacgtgctc tccggctacg agccgggcga cccctggtgg
     1201 gcgccgccac ccgagcggcc gttcgccgag gaggcggagc tcgagcccga accactgcgc
     1261 gtggccgtca cgacccaacc cccgctcgac gtcgccgtcg aaccggaatg ctcggcgacc
     1321 gccggggagg ctgccgagct gctcgccggg ctcgggcacg acgtcacgga ggcgacgccg
     1381 gagtggacga ccgacctcga gacgttcctg gtggtctggc agctcggccc ggcgctcttc
     1441 ggcgtgcgcg acctcgagct gctgaccccg atcaatcgga tgctggccga gcgcgcggcg
     1501 gcaacgtcgg cggtcgagta cgggtatgcg gtagcccgcc ttcacacgct cgcacgtcgc
     1561 gtcgtcggct tctgggaccg ctacgacata ctggtcacgc cagcgctcgc actcccgccc
     1621 gtcccgatcg gctggcagga ggaggagccc gacccgctgg agcagctctt ccgaggggcg
     1681 acctttacgc cgttcacggc gattgcgaat ctcaccgggc tcccggccgc gtccatcccc
     1741 ttcgcgtgga accgggccgg cctgccgctc ggcgtccagc tgatcgggcc ccccgcgggc
     1801 gaagcgctcc tgcttcgggt ctccgcccag ctcgagcgtg cgcggccgtg gctcgaccgg
     1861 aggccccccg aactggatcg cgcgtagaag aagaggcgtc cggaccggct ggccgcgcca
     1921 cggacgcctc ctgtgtggcg accctaccgc atgcggccgc cgaggccaag cgtccgaatg
     1981 cggatccttc gcccgggctg cgggtgcagc atcgcggccg cactgccgtt cgagatcgcg
     2041 accgacatgt tccggtagct gtcttcgtag agaatgacgt caccgggccg tgcatccgcg
     2101 aacgtacgtg cgctgacggc gtagtagcgc tccccgctga gatcgagctc gacgcgcgtt
     2161 cccggcacga caccgatctg ctggacgtcg tctctcgtca ggttgagggc gatgttgccg
     2221 aagctgtcgg cgtacagcat cgtcgcctcg acggagccgt cgccgaaaga gggttccggg
     2281 acgtcgagtc ggacgagcgc gtcgacggcg agcggcggtc cgagctgagc gggctcgaca
     2341 ccgcggacga gatgcgcagc gaccggcgcg aagatgtcgc ggccgtggaa cgtgcgcgag
     2401 atggtgtcga gccagtaccg ctcgttcgta agctcccgcg cctcgacgat gcctgccctg
     2461 tccgccgctg gcagaagcag cccgttgtcg ggcccgacaa agattcgccc ttcgccgtct
     2521 gtgagcgcga gcgcccggcg cgcgccgccg acccccggat cgaccaccgc caggtgcacc
     2581 ccgaccggca tgtacgggat cgtgttcgcg agcatcaacg ccccctgcag caccgcctgc
     2641 ggcgggatcc cgtgcgtgat gtcgatgatc tgcgcttctg gggcgatccg cttgatcaca
     2701 ccgtggcacg tcccgacgaa gtcatcaccg agcccgaaat cggtgaggaa ggtgatgaac
     2761 atgtgcggac gctagctcgc ttcggccgtc cgctccaccg cggccagcag accggcgacg
     2821 tcctgggtct ctgcctcggc gagcacctcc agccccgcgg cgcgagcagc gagggtggtc
     2881 tgggggccga tcgacacgac cggcggacgc agcgggagtg aggcgagcgc gcgcgcggcg
     2941 gacggtgagg ctacgacggc gaggtcgccc tccggcgcgg gatcgggccg cagctcggcg
     3001 gtgcgataga gggggacgaa atcagcgcgg agctcctcgg ccagcagccg gcgggctccc
     3061 tcggcgccgg cgaagagcac gcgccctgcc ggccgcggga gctcttcgag cagaccctcc
     3121 tgggtcgaca cggtggcgac gaggtcggcg ccgccgaacg ctgcggcagt cgcactgcca
     3181 atcgcggcca cgcgccgcgg cgttccgcgc atccgccgcc ggagctcgcg cgcgccgtgt
     3241 gcgctggtca acacgaccca gtcgta
//
LOCUS       DBNGNK010000303         3354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_410727,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000303 DBNGNK010000000
VERSION     DBNGNK010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_410727"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1264
                     /locus_tag="AC150V_08920"
     CDS             131..1264
                     /locus_tag="AC150V_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763078.1"
                     /translation="MARGVLAAALAALALTLTACGGNGEGLVVGAVDDVVRSPDPNEA
                     AALLEELVEAGFDAVAITSLWDPGLTAPTEDELAVQRTVAEQAEQRGLRLFVAVYHRG
                     SATTPLRSEARAAFASYAAALAAQVPELDDLIVGNEPNLNRFWLPQFAEDGESAAPAA
                     YLALLAATYDAVKALSDVRVWGGALAPRGGDRPGGSRPTHSPTRFIEELGQAYRASGR
                     DEPVMDGFVHHPYPDNSQVPVDLPHPRTTSIGLADYDKLVALLGDAFDGTAQPGSDLP
                     LLYGELGIETTVPADKESLYEGVELNKPVSEEEQAEAYRRALELASCQPTVVGLLIFH
                     VRDEPRLEGWQSGVRWVDGAAKSSQEPVREAAIEAREGRLCEA"
     gene            complement(1388..1609)
                     /locus_tag="AC150V_08925"
     CDS             complement(1388..1609)
                     /locus_tag="AC150V_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763079.1"
                     /translation="MESTLTEFGLGEDVLRRELEEELLLAMRAEGDVPTVHAIAHAVA
                     RVIQLDHMKAAEQLRAAGIDLPSTEPRGR"
     gene            1776..2909
                     /locus_tag="AC150V_08930"
     CDS             1776..2909
                     /locus_tag="AC150V_08930"
                     /inference="COORDINATES: protein motif:HMM:NF037429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VLRF1 family aeRF1-type release factor"
                     /protein_id="HIR3763080.1"
                     /translation="MIRWSELRSSLLSLARVRDPVGILSVTVGPAPRAESNRTPDWQI
                     ALRNALAGVRQDVSDSSLLDRRLEEIEPRLDALHEPFSADQSYAGFVALESGDVTEFT
                     LPVRLGTDARVAAVPHLLPLLGVLEDEKPSGLVSASQNEIEVLESELGRITPLEAIDL
                     EPSVGDWWPEMKGPAAANPARAQQVVSQRDRYERKLASLYSRQLEEGCRALARVARRR
                     RWARAVLAGDHRVTGPLEESLAQSASTITTESNFSGLREADVRSRLRTELSRLVECEA
                     VERVVKAIADEDAGGPGVTGLEGTVAALARGRVACLAIDPGRMFPGTVDPEGSLTPDE
                     AGRDLTDWIVAAALAVDADVLPVAGEAATVLADKGGLGAALRW"
     gene            complement(2919..>3354)
                     /locus_tag="AC150V_08935"
     CDS             complement(2919..>3354)
                     /locus_tag="AC150V_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR3763081.1"
                     /translation="ALIDTRTGLVLLYTVFALPFTVWMMYTYFRQMPPSLEEAALVDG
                     CTRIQALRKIAWPLAKPGVVSAAAFAFIFSWTEFLFAVVLARDHAVTLPVALAGMAAS
                     QQGNLFGEASALTMVSLIPALLLGILLQRHLVRGLTLGAVEG"
BASE COUNT          539 a         1176 c         1135 g          504 t
ORIGIN      
        1 tacacgggag gcttcacctt ctcgcccttc gagaatcgcg ccgatttccg ggacgtcgga
       61 gaaggccgca tcgccgtgac ctgtccctaa gtcgcacgcg cgcggaggaa caggcggcta
      121 cgctcggaac gtggccagag gggttctcgc cgccgccctc gcagcgctgg cgctgaccct
      181 caccgcgtgc ggtggcaacg gcgagggtct cgtcgtcggc gcggtcgacg acgtcgtccg
      241 ctcacccgac ccgaacgagg ctgcggcgct gctcgaggag ctcgtcgagg cgggcttcga
      301 tgccgttgcg atcacgagcc tctgggatcc ggggctcacc gcccccaccg aggacgagct
      361 cgccgttcag cgcaccgtcg ccgagcaggc cgagcagcgc ggcctacggc tcttcgtcgc
      421 cgtctaccac cgcgggtcgg cgacgacgcc cctccggtcg gaggcgaggg cggctttcgc
      481 ctcgtacgcg gccgctctgg ccgcccaggt gccggagctc gacgacctga tcgtgggcaa
      541 cgagccgaac ctcaaccgct tctggttgcc gcagttcgcc gaggacggag agagcgcggc
      601 accggccgcc tacctcgcgc tcttggccgc gacctacgac gcggtcaaag ccctctcgga
      661 cgttcgtgtg tggggcggcg cactcgctcc cagaggcggt gaccgtccgg gcgggtcgcg
      721 gccgacccac tcgccgaccc gcttcatcga ggagctcggc caggcgtacc gcgcaagcgg
      781 ccgcgacgaa ccggtgatgg acggcttcgt tcaccacccg tacccggaca attcgcaagt
      841 acccgtcgat ttgccgcatc cgcgaacaac gtcgatcggc ctggccgact acgacaagct
      901 cgtcgcgctg ctcggcgatg ccttcgacgg caccgcccaa cccggctcgg atctgccgct
      961 cctctacggg gagctcggca tcgagaccac cgttccggcc gacaaggagt cgctgtacga
     1021 gggtgtggag ctgaacaagc cggtctcgga agaagagcag gcggaggcct atcgacgcgc
     1081 tctcgagctc gcctcctgcc agccgaccgt cgtcggcctt ctcatcttcc atgtccgcga
     1141 cgagccacgt ctcgaaggct ggcagtcagg ggtgcgctgg gtcgacggcg cagcaaagag
     1201 cagccaggag cccgtgcgtg aggccgcgat cgaggcgcgt gaggggcgtc tctgtgaggc
     1261 ctgaggtccc gatcgaaccc acctcgttgc gatcaccagg aacggcgaag accgcagcct
     1321 cgaaccgggt atcccgcctc ggcataccgt tcgacagtgc tccgcggctg aacgccgggg
     1381 agattcgtca gcgacctcgg ggctccgtgg aaggaaggtc gatccccgcc gcgcgaagct
     1441 gttccgcggc cttcatgtga tcgagctgga tcacacgtgc gaccgcgtgg gcgatcgcat
     1501 ggactgtggg gacgtcgccc tcggcgcgca tcgccaggag caactcttcc tcgagctccc
     1561 tgcgcaggac gtcctccccc agtccgaact cggtcaacgt cgactccatg tcgcacctcc
     1621 tcaacgtctc tctccagcat ggccgagccg gagggcgggc gcatcagggt caacccggac
     1681 tgggcgtacg gacgcgagat ccgtggccac cttcggatac ggcatcggtg gacgccctga
     1741 tggcggctgg ctgcgcgttg gagatcattg aagtgatgat tcgctggtct gagcttcgct
     1801 cgtcgcttct gagcctggcc cgggtccgcg acccggtcgg catcctgtcc gtcaccgtcg
     1861 gaccggcgcc acgagccgag tccaaccgca ctcccgactg gcagatcgcg ctgcgcaacg
     1921 cccttgccgg tgtccgtcag gacgtctccg attccagtct gctcgaccga cgcctggaag
     1981 agatcgaacc gaggctcgac gccctgcacg agccgttctc ggcagaccag agctatgcag
     2041 gcttcgtggc actcgagtcc ggagatgtga ccgagttcac gctccccgtt cgcctcggca
     2101 ccgatgcacg cgttgccgca gtccctcacc tgctccctct gctcggggtg ctcgaggacg
     2161 agaagccgtc gggactcgtc agcgcatcgc agaacgagat cgaggtgctg gagtccgagt
     2221 tggggcgcat cacacccctc gaggcgatcg acctcgagcc gtcggtcggc gactggtggc
     2281 cggagatgaa ggggccggcc gccgccaacc ctgcccgcgc tcagcaggtc gtcagccagc
     2341 gcgatcgcta cgagcgcaaa ctcgcgagcc tgtattcgcg ccagctcgag gaggggtgcc
     2401 gggccctcgc gcgagttgcc aggcgccgca gatgggcgag agccgtcctg gccggggatc
     2461 atcgcgtcac cggcccgctc gaagagtcgc tcgcccagag cgcttccacg atcacgaccg
     2521 agtccaattt cagcgggctc cgagaagcag acgttcggag ccggcttcgt acggagctct
     2581 cccgtctcgt cgagtgcgag gcagtcgagc gggtcgtgaa agcgatcgcg gacgaggacg
     2641 caggcggacc tggggtcacg ggcctcgaag ggacggttgc cgcacttgcc cgcgggcgcg
     2701 tcgcctgcct cgccatcgac ccagggcgaa tgtttcccgg cactgtcgac cccgagggaa
     2761 gccttacgcc tgacgaggcc ggacgcgatc tcaccgactg gatcgtcgcc gccgcgctcg
     2821 ccgtcgacgc cgacgtccta cccgtggcgg gagaggcagc caccgtcctc gctgacaagg
     2881 gcggcctcgg cgccgcattg cgctggtgac cgagtcgcct agccttcgac ggccccgagc
     2941 gtgaggccgc ggacgaggtg gcgttgcagc aggatcccga ggagcagcgc cgggatcagc
     3001 gacaccatcg tcagagcgct cgcctcgccg aagaggttgc cctgctgcga ggcggccatg
     3061 cccgccagag cgacgggcag cgtgacagcg tggtcgcggg cgagcacgac cgcgaacagg
     3121 aactcggtcc aggagaagat gaacgcgaac gccgccgccg agacgacgcc cggcttcgcc
     3181 aacggccagg cgatcttgcg caaggcctgg atccgcgtgc acccgtccac cagcgccgcc
     3241 tcctccagcg agggcggcat ctgccggaag tacgtgtaca tcatccagac ggtgaacggc
     3301 agcgcgaaca ccgtgtacag gagcacgagg ccggttcgcg tgtcgatcag ggcg
//
LOCUS       DBNGNK010000304         6698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_410806,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000304 DBNGNK010000000
VERSION     DBNGNK010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_410806"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC150V_08940"
     CDS             complement(<1..191)
                     /locus_tag="AC150V_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763082.1"
                     /translation="MSERRYLAPLPLGGEEVPWSKGLMARALAATGLAPTPSYELARR
                     IDAHLAERGADVLDLDEIS"
     gene            complement(188..1477)
                     /locus_tag="AC150V_08945"
     CDS             complement(188..1477)
                     /locus_tag="AC150V_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate synthetase"
                     /protein_id="HIR3763083.1"
                     /translation="MALIDGEHYPPVVRQALEAVSYEVVAAVLVGGGEKLREGADYGV
                     PLAADIDVAIAEHAPDVVLDLSDEPVLGPVERMELASRVLVQGLPYEGADFRFEAPEF
                     ADFELPSLAVIGTGKRVGKTAVTGHIARLLARDRRVVVVAMGRGGPPEPEVIRVAPTV
                     EELVALSRSGRHAASDHLETAALAGVETVGCRRCGGGLAGAVTVSNVLAGAAIAVELG
                     PDLVVFDGSGAALPPVAVRRRVVVVGAHQAVEVAAGYLNGYRHRLADLVVLTMAEEDS
                     GHGELTTAIRRLVRPEVAIIATVLRPRPMASVTGREVAFFGTAPPSAHPRIAAHLRDA
                     YGAEVVHVSGNLADRQALSDELPQVHADCFLVELKAAAVDVVAEEAMARGIDVVLAAN
                     DVVALPTEQDLDYELRRLAEEAGTADETEALAGGRRR"
     gene            complement(1507..2313)
                     /locus_tag="AC150V_08950"
     CDS             complement(1507..2313)
                     /locus_tag="AC150V_08950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3763084.1"
                     /translation="MSANAEVFRIAHLSDLHCGGPYFEPNLLERAISEINDSGPDMVV
                     CSGDLTTFGFKHEYVLARSYLDRIECQDLVVVPGNHDSRNVGYVHFETLFGKRNSVLH
                     KGGVTVVAVDSTEPDLDHGQIGRGRYEWIEEAFAAAPAELRIFVLHHHLLPVPGTGRE
                     RNVVYDAGDAIECLQRAGVHLVLSGHKHVPYAWRLEDLFVVNTGTVSSLRLRGNTRPC
                     YNMIEVAGRHVDIWRRHPFHGEERIIQFSTTTFEYEKYTGRIEREVTTRS"
     gene            2404..2772
                     /locus_tag="AC150V_08955"
     CDS             2404..2772
                     /locus_tag="AC150V_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK family nuclease"
                     /protein_id="HIR3763085.1"
                     /translation="MPGETQRFRAFRGVEPPRFANRIEVECAKILDYYGVPWEYEPRT
                     FVLERDVKGRVASAFTPDFYLPEQELFVEVTVMKQSLVTRKNRKLRELRRQYPEVRVK
                     LFYRRDIELLAQRYRLKLAS"
     gene            2769..3344
                     /locus_tag="AC150V_08960"
     CDS             2769..3344
                     /locus_tag="AC150V_08960"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043174 - nucleoside salvage [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR3763086.1"
                     /translation="MTEPTQTDAHRIGDVYLSRDEIADRVTELGSEVERDLGARDPLL
                     IAPLKSSTVFLADLSRAIRAPHSFDLVELADYAGGSGGGVRLLKDIDMPIKDRHVLVV
                     QDVIDTGLTLHFLLRTLRLRQPASLAAVTLLDRPYRRLVDDLPLRYVGFTVPDELFAG
                     YGLGLEERWRALPDIRFVRAEALPAGATEAA"
     gene            complement(3351..4637)
                     /locus_tag="AC150V_08965"
     CDS             complement(3351..4637)
                     /locus_tag="AC150V_08965"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3763087.1"
                     /translation="MARWPALAAVLIGVCLLAAPAGGDPGDEKQRVDAQIGELRGKIE
                     QAEQEQGVLTSEIAAVSAHVREVQAQVDSEQQRLVVLEGQLASERDKLVRLNRLFESQ
                     TEQLHAYEAEHHAAVEQLEERVRQIYMEDTPDALSFVLGATSFSDLINHVELLSDIGR
                     QDSRIAARVAQAERETREARAATSRTRAGVRKTTRMISARVDEQRALRDGLVASRDEL
                     IAAESEKQRTLAAVQSEHDHAVVDLGALEAESAALGAEIRAAQEAAAARAAEAAAAAA
                     AAAAAASPQPAPLSAPPPPQSAAASGSGVVAASGFIFPVSGPVVSGFGPRGGRMHEGI
                     DIIADAGTPVVASAAGTVIRSGWGSGYGNVVVIDHGNGLSTTYAHNSALAVGVGAVVG
                     QGTVIAFVGSTGNSSGPHVHLEFRINGVAVDPFGYL"
     gene            complement(4652..4996)
                     /locus_tag="AC150V_08970"
     CDS             complement(4652..4996)
                     /locus_tag="AC150V_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763088.1"
                     /translation="MTLALIIGALLAAGCVVVVALPYLREPKPQNDVLEQPDVLGKRQ
                     LELAEARDRALAALKELEFEHRAGKISDEDYRAQVGPLRRAAAQALRSLEPRTSTGEP
                     AAEQLPQGQPTE"
     gene            complement(4993..>6698)
                     /locus_tag="AC150V_08975"
     CDS             complement(4993..>6698)
                     /locus_tag="AC150V_08975"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR3763089.1"
                     /translation="WLLVLTAYGALAVTLGRRRAPGLVVWVVPVMAAIATGFAWLVVG
                     VASPFATQVAPADGFGLNPSLQNPYMATHPPMLYLGYVGLAVPFAFAMGALLAGRTDE
                     RWIVATRRWTLFAWVTLGLGQLIGAKWAYEEVGWGGYYAWDPVENAALMPWLAATAFL
                     HSVMIQEKRGMLKVWNVTLVSFAFCLSIFGTFITRSGIVNSIHSFTQSSIGAWFLSFI
                     IIAVVFSIAMIVYRLPMLRAETKLESLVSREATFLYNNLLLIALCLTILWGVAWPMLS
                     EAVRGEAVVVGRPYYDFFLRIFGLPLLFLMGIGPLIAWRRASLRSLAKTFAVPTAAAL
                     TAGVVLVSFGAGESIPGLIAYTFSAFVLATIALEFARGTRARRALSSESWPAALTSLI
                     GRNRRRYGGYIVHAAIVLLAVGVAGSSAYDSVREGRLESGQALEVGGYTLVYRGIEER
                     QAANATEVRAQFDVARGDRSLGSIEAGKNAYDVEQQVSNEAGIRSDYLTGEDLFVIAE
                     QVNTDGSVIVRVFVKPLVNLIWLAGIVFLFGSLITLWPDPREERRLVTRYREIGAPAE
                     G"
BASE COUNT         1090 a         2349 c         2221 g         1038 t
ORIGIN      
        1 tcgctgatct cgtcgaggtc gagcacgtcg gcgccgcgct ccgccaggtg ggcatcgatc
       61 cggcgcgcga gctcgtagga cggagtcggc gccaggccgg tcgccgcgag ggcgcgggcc
      121 atcaatccct tcgaccacgg cacctcctcc ccaccgagcg gcagcggagc cagatagcgc
      181 cgttcgctca tcggcggcgc cccccggcga gcgcctccgt ctcgtcggcc gtgccggcct
      241 cctcggccag acggcggagc tcgtagtcga ggtcctgctc cgtgggcagg gcgacgacgt
      301 cgttcgcggc gagcaccacg tcgatcccgc gagccatcgc ctcctcggcc accacgtcca
      361 ccgccgccgc cttcagctcg acgaggaagc agtcggcgtg aacctgcggc agctcgtcgg
      421 acagggcctg gcggtctgca aggttgcccg acacgtgcac cacctcggcc ccgtacgcat
      481 cacgcaggtg agcggcgatc cgcggatgcg ccgacggcgg agcggtgccg aagaaggcga
      541 cctcgcgacc ggtcaccgac gccatcgggc ggggcctcag caccgtcgcg atgattgcga
      601 cctccggccg gacgaggcgc ctgatcgccg tggtgagctc cccgtgcccc gaatcctcct
      661 cggccatcgt cagcacgacc aggtcggcga gtcggtgacg gtagccgttc aggtagccag
      721 cggccacttc gacggcctgg tgtgcgccca ccaccaccac ccggcgcctg accgccactg
      781 gcggcagcgc cgctccgctc ccgtcgaaga cgacgaggtc cgggccgagc tccactgcga
      841 tcgcagcccc cgcgaggacg ttcgagacgg tgacggcgcc ggcgagaccg ccgccacatc
      901 gccggcagcc gactgtctcg actccggcga gggcagccgt ctcgagatga tcggaggctg
      961 cgtgccgtcc gcttcgcgac agcgccacga gctcctcgac cgtgggagca acccggatga
     1021 cctccggctc gggcggaccg ccgcgcccca tggccacgac gaccacgcgc cggtcgcgtg
     1081 cgagcaggcg ggcgatgtgc cccgtcacgg ccgtcttgcc gacgcgcttc ccggtcccga
     1141 tgaccgcgag tgacgggagc tcgaagtcgg cgaactcagg ggcctcgaag cggaagtcgg
     1201 ccccctcgta gggcagcccc tgcacgagca cgcggctcgc cagctccatc cgctcgaccg
     1261 gccccagcac gggctcgtcg gagagatcga gcacgacgtc gggcgcgtgc tccgcgatcg
     1321 ccacatcgat gtcggccgca aggggaaccc cgtagtcggc gccttcccgg agcttctcgc
     1381 cgccgccgac gagcacggct gcgacgacct catacgagac cgcctcgagc gcctggcgca
     1441 cgacgggcgg gtagtgctcg ccgtcgatca gcgccactgc acgcgcctct cgttgcggcg
     1501 gccgactcac gaccgcgtcg tcacctcgcg ctcgatccgc cccgtgtact tctcgtactc
     1561 gaacgtggtc gtcgagaact ggatgatccg ctcctcgccg tggaacggat gccgccgcca
     1621 gatgtccacg tggcgaccgg cgacctcgat catgttgtag caggggcgcg tgttgcccct
     1681 cagccgcaat gaggaaacgg ttcccgtatt gacgacgaag agatcctcga gtcgccacgc
     1741 gtacggcacg tgcttgtggc cggacaagac caggtgcaca ccggctcgct gcaggcactc
     1801 gatcgcgtct ccggcgtcgt agacgacgtt ccgctccctc cctgtgcccg gcaccggcag
     1861 cagatggtgg tggagcacga agatcctgag ttcggccggc gccgccgcga acgcttcctc
     1921 gatccattcg tatcgacccc ggccgatctg accgtggtcg aggtcgggct cggtcgagtc
     1981 gaccgccacg acggtcacgc ctcccttgtg cagcaccgaa ttgcgcttcc cgaacagtgt
     2041 ctcgaagtgc acatagccga cgttgcggga atcgtgattc ccgggaacca caacgaggtc
     2101 ctggcattcg atcctgtcga ggtagctccg cgcgagcacg tactcgtgct tgaagccgaa
     2161 cgtcgtgagg tcgcccgagc acacgaccat gtcggggccg gagtcgttga tctcggagat
     2221 cgcgcgttcg agcagattcg gctcgaagta cggccccccg cagtgcaggt ctgagaggtg
     2281 cgcgatgcgg aagacctcgg cgttggcaga catggcggca gtgtagaggc ttcccgaaac
     2341 gcccttgccc gcggccggca gccgccgccg gcgtcgtccg ggtgcctcgc tagcgttgcc
     2401 atggtgccgg gcgaaaccca gcgcttccgg gcgttccgtg gtgtcgagcc accgcgtttt
     2461 gcgaaccgga tcgaggtcga gtgcgcgaag attctcgact actacggtgt gccgtgggag
     2521 tacgagcctc gtacattcgt gctcgagcgt gacgtgaagg gccgggtggc gagtgcgttc
     2581 acgcccgact tctacctgcc cgagcaggag cttttcgtcg aggtcacggt gatgaagcag
     2641 tcgctcgtga cccggaagaa ccgcaagttg cgggagctcc ggcgccagta tcccgaggtc
     2701 cgggtgaagc tcttctaccg aagggacatc gagctgctcg cccagcggta caggctgaag
     2761 ctcgcctcgt gaccgagccg actcagaccg acgcgcaccg gataggcgac gtctacctct
     2821 cgcgggacga gatcgccgac cgcgtcaccg aactcggcag cgaggtcgaa cgcgatctcg
     2881 gcgcccgcga cccgttgttg atcgcgcctc tgaagtccag cacggttttc ctcgccgacc
     2941 tctcgcgcgc gatccgagcc ccacattcgt tcgacctcgt cgagctggcc gactacgccg
     3001 gcggaagcgg cggcggcgtc cgtctcctga aggacatcga catgccgatc aaggatcgtc
     3061 atgtgctcgt cgtccaggac gtgatcgaca ccggtctcac actccacttc cttcttcgca
     3121 ccctgcggct gcggcagccc gccagcctcg ccgctgtgac gttgctcgac cgcccctacc
     3181 ggcgactcgt cgacgatctt ccgttgcgct acgtgggctt cactgtcccc gacgagctct
     3241 ttgcggggta cggcctcgga ctcgaggagc ggtggcgtgc tctgccggac atccgcttcg
     3301 tgcgggccga ggcgctgccg gcaggggcta ccgaagctgc ctagggccgc ttacagatag
     3361 ccgaacggat cgacggcgac accgttgatc cggaactcga gatgaacgtg cggaccgctc
     3421 gagtttccgg tgctgccgac gaaggcgatc accgttccct gcccgacgac ggccccgact
     3481 ccgaccgcca gcgcggaatt gtgcgcgtag gtcgtggaca agccgtttcc atggtcgatg
     3541 acgaccacgt ttccgtaccc gctgccccag cccgaccgga tcaccgtgcc cgccgcggaa
     3601 gccacaaccg gtgttcccgc gtcggcgatg atgtcgattc cctcgtgcat gcgtccgccg
     3661 cgcgggccaa agccgctcac gacggggccg gagaccggga agatgaaccc tgacgccgcg
     3721 acaactccag acccgctcgc agccgcggac tgtggcggag gtggcgcgga cagtggagcg
     3781 ggctgcgggg atgccgcagc agccgctgca gccgctgcgg cagctgcctc ggcggccctc
     3841 gcggcggcgg cttcctgggc cgcgcggatc tcggcgccca gcgccgcgct ctcggcctcg
     3901 agcgccccga gatcgacgac cgcgtggtcg tgctccgact gaaccgccgc cagcgttcgc
     3961 tgcttctcgc tttcggcggc gatcagctcg tcgcgactcg cgacgagacc gtcgcgcagc
     4021 gcccgctgct cgtccacccg cgccgagatc atccgggtcg tcttccgcac gccagccctt
     4081 gtgcgagagg tcgccgcgcg cgcctcccgc gtctcccgtt ccgcctgcgc gacgcgggcc
     4141 gcgatccgcg agtcctggcg accgatgtcg ctgagcagct cgacgtggtt gatcaagtct
     4201 gagaacgacg tggcgccgag gacgaaggac agggcgtcgg gcgtgtcctc catgtagatc
     4261 tggcgcaccc gctcctccag ctgctcgacc gcagcgtggt gctcggcttc gtaggcgtgg
     4321 agctgttcgg tttggctctc gaagagccgg ttcagccgca cgagcttgtc tctctcgctc
     4381 gcaagctgac cctccaggac aaccagacgc tgctgttcgg agtcgacctg agcctgcact
     4441 tcgcgcacat gcgcgctgac agcagcgatc tccgaggtca gcacgccttg ttcctgttcc
     4501 gcctgctcga tctttccgcg gagctcaccg atctgtgcat cgacccgctg cttctcgtcg
     4561 ccgggatcac cgccggcagg ggctgccagc agacagaccc cgatgaggac cgcagcaaga
     4621 gctggccagc gagccatcgg tgacactttc gctactccgt cggctgtccc tgcggcagct
     4681 gctcggcggc aggctcaccg gtcgaggttc gcggctcgag cgagcgaaga gcttgcgccg
     4741 ccgctcgtcg aaggggcccg acctgggcgc ggtagtcctc gtccgagatc ttgccggcac
     4801 ggtgctcgaa ctcgagctcc ttcagagccg ccagcgcacg gtcgcgtgcc tcggcgagct
     4861 ccagctgccg cttgccgagc acgtcgggct gctcgaggac gtcgttctgc ggcttcggct
     4921 cgcgcagata cggcagcgcg acgaccacca cgcagccggc ggcaaggaga gctccgatga
     4981 tgagagcgag cgtcaccctt ccgccggcgc gccgatctcg cgatagcgcg tgacgagccg
     5041 cctttcctcg cgcgggtcgg gccagagcgt gatcagcgag ccaaagagaa acacaattcc
     5101 cgcgagccag atcaggttca cgagcggctt cacgaagacg cgcacgatga ccgagccgtc
     5161 ggtgttcacc tgctccgcga tcacgaacaa atcctccccc gtcagatagt cggagcgtat
     5221 gcctgcctcg ttggaaacct gctgctcgac gtcgtaggcg ttcttccctg cttcgatgct
     5281 tccgaggctt cgatctcctc gggcaacgtc gaattgtgcg cgcacctccg tggcgttcgc
     5341 cgcttgccgc tcctcgattc ctctgtagac gagggtgtag ccgccgactt cgagcgcctg
     5401 gccgctctcg agccgccctt cccgaacgga gtcgtaggcg ctggagcccg cgaccccgac
     5461 ggcaagcagc acgatcgcgg cgtggacgat gtacccgccg tagcgccggc gattccgtcc
     5521 gatcaaggac gtgagcgcgg ccggccacga ttcgctcgac agcgctctcc gtgcgcgtgt
     5581 gccgcgggcg aactcgagag cgatcgtcgc caggacgaac gccgagaacg tgtacgcgat
     5641 gagtcccgga atcgactcgc cggccccgaa ggagacgagc acgactccgg ccgtgagcgc
     5701 ggccgccgta gggactgcga aggtcttcgc gaggctccgc aaggacgctc gccgccacgc
     5761 gatcagcggc ccgatgccca tcaggaagag gaggggtaag ccgaagatac ggaggaagaa
     5821 gtcgtagtag ggacggccga cgacgaccgc ctcgccgcgg acggcttccg agagcatggg
     5881 ccaagcgact ccccacagga tcgtgaggca gagagcgatc agcaacaggt tgttgtagag
     5941 gaacgtcgcc tcgcgcgaga cgagcgactc gagtttcgtc tcggcgcgga gcatgggcag
     6001 ccggtagacg atcatcgcga tggagaagac gaccgcgatg atgatgaagg acaagaacca
     6061 cgcgccgatc gagctctgcg tgaacgagtg aatggagttg acgatgcccg atcgcgtgat
     6121 gaacgtcccg aagatggaga ggcagaaggc gaagctgacg agcgtgacgt tccagacttt
     6181 cagcatgccg cgcttctcct gaatcatcac cgagtgcagg aacgcggtcg ccgcgagcca
     6241 cggcatcaga gcggcgttct cgactgggtc ccaggcgtag tagccgcccc agccgacctc
     6301 ctcgtacgcc cacttcgccc cgatcagctg gcccagcccg agcgtcaccc aggcgaagag
     6361 cgtccagcga cgcgtggcca cgatccagcg ctcgtcggtg cggccggcga gcagtgcccc
     6421 catcgcgaaa gcgaagggaa cggcgagccc cacatagccg agatagagca tcggcgggtg
     6481 ggtcgccata taggggttct gcaggcttgg attgagcccg aatccgtcgg caggcgcaac
     6541 ctgcgtggcg aatggacttg cgacaccgac gacgagccag gcgaatcccg tcgcgatggc
     6601 cgccatgacc ggtaccaccc agacgacgag gcccggcgcg cgccggcggc cgagggtgac
     6661 tgcgagcgcc ccgtaggcgg tgagaaccag caaccaca
//
LOCUS       DBNGNK010000305         7724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1845212,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000305 DBNGNK010000000
VERSION     DBNGNK010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1845212"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="AC150V_08980"
     CDS             complement(<1..221)
                     /locus_tag="AC150V_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763090.1"
                     /translation="MLAVVGIALLFLLPPPWNLFGVPVAIVGAITPVLFWHRRVRDIG
                     VQAGAETLVGATATVVAPCAPSGQVRFRG"
     gene            522..950
                     /locus_tag="AC150V_08985"
     CDS             522..950
                     /locus_tag="AC150V_08985"
                     /inference="COORDINATES: protein motif:HMM:NF049235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7482 domain-containing protein"
                     /protein_id="HIR3763091.1"
                     /translation="MKRLALLGALLVTAIGFAVPQLAGAQSEPVNTSVTHFAQPTTLG
                     YYRGKTVEYYDLGPVKLKAGNKVAPIWAFTNGANGQFNIIDTVPGQKSYTPLWGVRLV
                     TWKDGVTPRVLRSKTAVDKALKAGDVTVKAMPIVVNCPVL"
     gene            complement(1024..1968)
                     /locus_tag="AC150V_08990"
     CDS             complement(1024..1968)
                     /locus_tag="AC150V_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763092.1"
                     /translation="MSGARLIEGRAVRLCAFAAMLVALVLLGASQAAASQRVGLVDAS
                     EVVLRVDDRGAALVLYTTPAGVGRRVVASGGIDAAQPGSPQPKLDLDYAGGRGTWSFR
                     DHCRPYDGPLLAHLVAACRAPDGSYWALQRWQRVQPLRGVRPFRPEHSSFELRISHWS
                     GPLGELEVSPNWTYSGTMQGLFGRLTYRGVPQFGVRTPSASRSDAHARYVYIDTFNSP
                     YGPGWRREAAKVTHRGTGGFCYTFAPIPPPSGYPNEMPTVPGIGERHRVTVVGPGLTP
                     DLQWVGEGLGAFDPGADERFNAVFDRLLAGDRPCRSER"
     gene            2304..3359
                     /locus_tag="AC150V_08995"
     CDS             2304..3359
                     /locus_tag="AC150V_08995"
                     /inference="COORDINATES: protein motif:HMM:NF024724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3763093.1"
                     /translation="MIETADAYAETAHAGQVRKGTSIPYIEHPREVAGLLSQVGARDE
                     VVAAGLLHDTVEDTPATLDEIGDRFGAEVAEIVAAVTEDKTKSWERRKRYTITKLHSA
                     SEDVLLVTAADKVANARGMRKGGNEVWNRFRRPYPLQRRYYRGVVQALEARADGGPLG
                     TLTASLRVEVDAVFPRLDLPDLDALLPTVLRRATGRDSSIHGEEHWRAVAATGLALVE
                     HEPLADPLVVACFGLLHDTMRESDGFDPDHGRRSERFARELLAEGTLALADYQADALA
                     DALARHADGETTEAPTEGVCWDADRLHLPRIGIEPRRELLSTSIAALREAVAEASAVR
                     ADPPSWAASFERAFALS"
     gene            3473..4174
                     /locus_tag="AC150V_09000"
     CDS             3473..4174
                     /locus_tag="AC150V_09000"
                     /inference="COORDINATES: protein motif:HMM:NF018822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HIR3763094.1"
                     /translation="MPKAAAPLLALVAALALVAAGCGGGSDEGASAEELVAESAARTG
                     QLGSFRFTLDVQNVPITAEGLQITSAEGEVAVPDMAQADVTGNFAGVSLTTQVIAIGD
                     DVWLKNPLSGDWESIDVSTTPVALLDPAKGILGVMAGVKGARDIGSDELDGITVRKVE
                     GTAAAVAVAPLVATSASEGDVPVVLWIGEEDKLLRRVDVTGPVAGDEPEDALRSFEIS
                     RFDEPVTIEPPEGSG"
     gene            4195..4857
                     /locus_tag="AC150V_09005"
     CDS             4195..4857
                     /locus_tag="AC150V_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763095.1"
                     /translation="MPTLLAAAGVARSYGRRGARAPTDIELRAGEVVALVGPNGAGKS
                     TLLTMLAGALPPTRGTVLPPAPEVRVGWAPQRPALYGHLSAHENLVLFARLARLDDPG
                     AAATSSLERMSLPAGDQRASQLSVGNQQRLNLALALIGDPAVLLLDEPTASLDPAHRR
                     RLWELLEQLRGDGGSALLATHAFDEVERLADRVVVLLDGEVAFAGTPGDVVRAMPELA
                     GA"
     gene            4854..6197
                     /locus_tag="AC150V_09010"
     CDS             4854..6197
                     /locus_tag="AC150V_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763096.1"
                     /translation="MTAIGLLLGKDLRRLARSPLLLAALVVYPLLIALLVGLVVRYAG
                     ERPRIALVDEAGLPRTIQLGERRFDLRALFENAAEVDLARMSAEQAERELSTGRALAS
                     LTIPPDFTSKLRRPNESPRIVLRTSEDAIGTQAVEKMRALTYAINLKLQQAFIETNLD
                     YVDLLKRGGTGQLGESTVTIIGLQNAQKELERLAESSDPDVATAAAELAGFVEQVDGA
                     VEQVGEYLQATANPIELVTEEDAGRTWLLSAQVQAYALALALAFVAVLLGAASITSER
                     EERTLGRLARGLVGGTSLVAEKVLLVGGLGAGVGLLLALVFGVVVEIGGVSGGQPWQR
                     VPVLALGLVLAAGAFGALGVLLGTLARDASAAMLLALLVGLPVVLLGVIPAGTFALSD
                     VVSALVPFGHAVDFATAALYEDDPLGPILRAAAWLAGLTVAFAALARLSVRRLLV"
     gene            6244..6516
                     /locus_tag="AC150V_09015"
     CDS             6244..6516
                     /locus_tag="AC150V_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763097.1"
                     /translation="MCCLFLLAFGIGPRVALFFVWIFGDRVQDAFDSFAWPLLGLLFL
                     PWTTLLYVIVWQSGGLSGWGWFVVGLGVLLDLGSYLGRLGHRRAGF"
     gene            6857..>7724
                     /locus_tag="AC150V_09020"
     CDS             6857..>7724
                     /locus_tag="AC150V_09020"
                     /inference="COORDINATES: protein motif:HMM:NF045284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763098.1"
                     /translation="MSVAGLATGRDRLLRELLELISERLPPDRADLVGRFATLYGRRL
                     PAEAAETLTADELFGQVLGAFELADNRGVEPIAVRVFNPTLAGDGYTTVGSVIETNTP
                     DSPFLVDSVSEELDARELGIRLLLHPVLGIERDDAGRIAGVTSALQAESLESVMHFEV
                     DRHLAAPELSDLAAATLRILRDVQRAVGDFEPMRAKVATMIEAAAGSGARYEQDEIDE
                     TVAFLEWLDDDNFVYVGYREYEIVGEGAERAIRVKPGSGLGVLSDEATSTFAEPVLLS
                     KIDDDLRPRVEGG"
BASE COUNT         1114 a         2741 c         2717 g         1152 t
ORIGIN      
        1 tcgcccctga agcgcacctg gccgctcgga gcgcacggcg caaccaccgt cgccgtcgcg
       61 ccgacgagcg tctcggcgcc cgcctgcacg ccgatgtcgc gcacacgcct gtgccagaac
      121 agcaccggag tgatcgcgcc cacgatcgcg acgggcacgc cgaagagatt ccagggcggc
      181 ggaagcagga agagcagcgc gattccgacc acggcgagca tcaaatcgag agtagcgcgt
      241 ccgagggtga tcgcgttgtc tctcggctcc ccgtcctcca aagtccaacc gggatgcctg
      301 ctcggtcgag gaggctcagt tcgacgcttc gcttcgtcct cggcgacgtt cgagctggtc
      361 aagaagggcg cctgaaaacg gcctgttgtg ccgtccggcg atcgggctgt tcttacgggc
      421 gcatgaattc gagccacgtc accccgtgtt cagggccagg aaagagggcc gcaagaactt
      481 cctcctaccg tcggtgagtc ccaccactca ggaggaccgg aatgaagaga cttgcactcc
      541 tcggtgcgct gctcgtgacc gcgatcggct tcgccgtgcc gcagctcgcg ggcgcgcaga
      601 gcgagcccgt gaacacgtcc gtcacgcact tcgctcagcc gacgacgctc ggctactacc
      661 gcgggaagac ggtcgagtac tacgacctcg gcccggtgaa gctcaaggcc ggcaacaaag
      721 tggcgccgat ctgggccttc acgaacggtg cgaacggcca gttcaacatc atcgacaccg
      781 tgccgggcca gaagtcctac acgccgctct ggggcgtccg gctcgtcacc tggaaggatg
      841 gggtcacgcc gcgcgtcctg cgctcgaaga cggcggtcga caaagcgctg aaggccggcg
      901 acgtgacggt gaaggcgatg ccgatcgtcg tcaactgccc cgtgctctag cgctcccctc
      961 tacgcaccac cgaggcagtc ccgcagtcgc ggggctgcct cgccacggga cgcgagcagc
     1021 gcctcagcgc tcgctgcggc agggacgatc gccggcgagc agtcggtcga acacggcgtt
     1081 gaaccgctcg tctgcgcccg gatcgaacgc cccgagcccc tcgccgaccc actggagatc
     1141 cggcgtcagg cccgggccga cgacggtgac gcggtggcgc tcaccgattc ccggcaccgt
     1201 gggcatctcg ttcgggtagc cggaaggcgg cggaatcggc gcgaacgtgt aacagaagcc
     1261 gccggtgccc ctgtgtgtga ccttcgccgc ctcgcgcctc cagcccggcc cgtagggcga
     1321 gttgaaggtg tcgatgtaga cgtagcgcgc gtgggcgtcc gagcgggacg cagacggcgt
     1381 tcgcacgccg aactgcggca cgccgcggta ggtcaggcgg ccgaagagcc cctgcatcgt
     1441 gccgctgtag gtccagttcg gcgagacctc tagctccccg agcggtccgc tccagtgcga
     1501 gatccggagc tcgaagctcg aatgctccgg gcgaaagggg cgcactccgc ggagcggctg
     1561 cacccgctgc cagcgctgca gcgcccagta cgagccgtcc ggcgcccggc aggctgcaac
     1621 gaggtgcgcg agcagcggcc cgtcgtaggg gcgacagtgg tcgcggaagc tccacgtacc
     1681 gcgcccgccc gcgtagtcga ggtcgagctt cggctgcggg ctgcccggct gggcggcgtc
     1741 gatgcccccc gacgcgacca cgcgccggcc gaccccggcg ggggtcgtgt agaggacgag
     1801 cgctgcaccg cgatcgtcca ccctgaggac gacctccgac gcgtcgacga gccccacgcg
     1861 ctggctcgcg gccgcctgag aggcacccag gaggacgagc gcgaccagca tggcggcgaa
     1921 cgcgcacagg cgcaccgcgc gcccctcgat caggcgggct cctgacaacg gacgggaccc
     1981 taacatcccc cttcttgtcg gccgatcgac gcgggactgt agaccagacg ctctgatcgc
     2041 gacaactgcc acgagcggcg ggtgcgcggt actcggcccc gctgccgctc gattccttca
     2101 cgctcggctg gacgacgctg cggctctagg gcccggtcga gacgggcgtc gcggccgggg
     2161 ccgctgtcgg gagagggagc gaccccggtc gccacgcccg tcgattccgt cgtccgaacg
     2221 ggcgacgctc atgaccttcg tcacgaattg tgtcgcatcc ttccccgcac gtcgacacac
     2281 ggcccgtcgg gcaggatgca gaggtgatcg aaaccgcgga cgcctacgca gagactgccc
     2341 acgccggtca ggtgcgaaag gggacgtcca tcccctatat cgagcacccc cgcgaggttg
     2401 cgggactcct gagccaagtc ggcgcccgcg acgaggtcgt cgcggctgga ctgctgcacg
     2461 acaccgtcga ggacacgccg gcgacgctcg acgagatcgg cgatcgcttc ggtgccgagg
     2521 tagcggagat cgtggcggcg gtcaccgagg acaagacgaa aagctgggag cggcgcaagc
     2581 gatacacgat cacgaagctg cactcggcca gcgaggacgt gctgctcgtg acggccgccg
     2641 acaaggtggc gaatgcccgg ggaatgcgaa agggaggcaa cgaggtctgg aaccgctttc
     2701 gccgccccta ccctctgcag cggcgctact acaggggcgt cgtgcaggcg ctggaggcgc
     2761 gggcggacgg cggcccgctc ggcacgctca ccgcttcgct tcgagtcgag gtcgacgccg
     2821 tcttcccccg gctcgacctg ccggatctcg acgctctcct cccgaccgta ctgcgtcgcg
     2881 ccaccgggcg cgactcctcg atccacggcg aggagcactg gcgcgccgtc gccgctaccg
     2941 gtctcgccct agtcgagcac gagccgctcg ccgacccgct ggtggtcgcc tgcttcgggc
     3001 tactccacga cacgatgcgc gagagcgacg gcttcgatcc ggatcacggc cggcgctccg
     3061 agcgttttgc gcgcgaactg ctggccgagg gaacgctggc actggccgac taccaagccg
     3121 acgcgctcgc ggacgccctt gcgcgtcacg cggacggcga gacgacggag gcgcccaccg
     3181 aaggcgtgtg ctgggacgcc gaccgcctgc acctgccgcg gatcgggatc gagccaaggc
     3241 gggagcttct ctccacctcg atcgcggcgc ttcgcgaggc cgtcgccgaa gcgtcagcag
     3301 tgcgcgcgga cccgccgagc tgggcggcta gcttcgagcg cgccttcgct ctcagctgat
     3361 cgcaacagcg cgtcgcagca aggcgtctgc tccggaaccg cacgggacgc acggctccgc
     3421 ctcaccgccg ggagtcacgc cgccccgccc gggtggtttc attcagtgcg tcatgccgaa
     3481 agcagcagcg ccactcctcg cccttgtcgc cgccctcgcg ctggtcgcgg cgggctgcgg
     3541 cggcggcagc gacgagggcg cgtcggccga ggagctggtc gctgagagcg ccgcgcgcac
     3601 cggccagctc gggagctttc gcttcacgct ggatgtccag aacgtcccca tcactgccga
     3661 gggtctccag atcacctccg ccgaaggcga ggtcgccgtt ccggacatgg cgcaggccga
     3721 cgtcaccggc aacttcgcgg gtgtctcgct gacgacgcag gtgatcgcga tcggcgacga
     3781 cgtctggctg aagaatccac tcagcggcga ctgggaatcg atcgatgtca gcacgacgcc
     3841 cgtcgcgctc ctcgaccctg cgaagggaat cctcggcgtg atggcggggg tcaagggagc
     3901 gcgcgacatc ggcagcgacg agctcgacgg gatcacggtc cgcaaggtgg aaggcacggc
     3961 cgcggcagtt gccgttgcgc cactcgtcgc gacgtccgcg tccgagggcg acgtgcccgt
     4021 cgtcctctgg atcggcgagg aggacaagct gttgcggcgc gtggacgtca ccggccccgt
     4081 cgccggggac gagcccgagg atgcgcttcg ttcgttcgag atctcgcgct tcgacgagcc
     4141 ggtcacgatc gagccgccag agggaagcgg gtagtcgcgg cgacctcaac aggtgtgccg
     4201 acactcctgg ccgcagccgg ggtggcgcgg tcgtacggcc gccgcggcgc gcgcgcgccg
     4261 accgacatcg agctccgggc gggcgaggtc gtggcactcg tcggcccgaa cggggccggc
     4321 aagtcgacgc tgctgaccat gctggcgggc gcgctgcccc ccacacgcgg caccgtcctg
     4381 ccgcctgcgc cggaggtccg cgtcggctgg gcgccgcagc gccccgccct ctacggtcac
     4441 ctgtcggcac acgagaacct cgtcctcttc gcacggctcg cgcgcctgga cgatcccgga
     4501 gccgcggcca cctcgtcgct cgagcggatg agtcttccgg ccggagacca acgcgcctca
     4561 cagctctcgg tcggcaacca gcagcggctc aacctggcgc tcgcgctgat cggagacccg
     4621 gccgtccttc tgctcgacga gccgacggcc tcgctcgacc ccgcgcatcg acggcggctg
     4681 tgggaactgc tcgagcaact gcgcggcgac ggtggttcgg ctctgctggc cacgcacgcc
     4741 ttcgacgagg tcgagcgcct cgccgaccgc gtcgtcgtcc tgctcgacgg agaagtcgcc
     4801 ttcgccggta cgcccggtga tgtcgtcaga gcgatgccgg agctggcggg cgcatgaccg
     4861 cgatcggcct actgctcggc aaggacctgc ggcgcctcgc tcgctcgccc ctcctcctgg
     4921 ccgcccttgt cgtctatccg ctcctgatcg ccctgctcgt cggtctcgtc gtccgctacg
     4981 cgggggagcg acctcgcatc gcgctggtcg acgaggcggg cctgccgcgc acgatccagc
     5041 tcggcgagcg ccgcttcgac ctacgggcgc tcttcgagaa cgccgctgag gtcgacctcg
     5101 cgcgtatgtc ggccgagcag gccgagcggg agctctccac cggtcgagcg ctcgcctcgc
     5161 tcacgatccc gccggacttc acgagcaagc tccgccggcc gaacgagagc ccgcggatcg
     5221 tcctccgcac gagcgaggac gcgatcggga cgcaggccgt cgagaagatg cgtgcgctca
     5281 cctacgccat caacctgaag cttcagcagg cgttcatcga gacgaacctc gactacgtcg
     5341 acctgctgaa gcgcggcggc acggggcagc tgggcgagtc cacggtgacg atcatcgggc
     5401 tgcagaacgc ccagaaagag ctcgagcggc tggccgagtc gagcgatccc gacgtcgcga
     5461 cggcggcagc ggagctcgcc ggcttcgtcg agcaggtgga cggcgccgtc gagcaggtcg
     5521 gcgagtacct gcaggcgacg gcgaacccga tcgagctggt cacagaggaa gacgcggggc
     5581 gaacgtggct cctgtccgcg caggtgcagg catatgcgtt ggcgctggcg ctggccttcg
     5641 tcgccgtgct gctcggcgcc gcgtcgatca cgagcgagcg cgaggagcgg acgctcggcc
     5701 ggctcgcccg cgggcttgtc ggcggcacca gtctcgtcgc cgagaaggtg ctcctggtcg
     5761 gcgggctcgg cgccggcgtc gggctgctgc tggcgctcgt cttcggagtc gtcgtggaga
     5821 tcggcggtgt ctcgggtggg caaccctggc agcgcgtccc cgtcctcgcg ctcggcctcg
     5881 tcctcgcggc cggcgcgttc ggggctctcg gcgtgctgct cggcacgctc gcacgcgatg
     5941 cgagcgcggc aatgcttctg gcgttgctgg tcggtctgcc ggtcgtcttg ctcggagtca
     6001 tccccgcggg gacgttcgcg ctctcggacg tggtctctgc actcgtgccc tttgggcacg
     6061 cggtcgactt cgccacggcc gcgctgtacg aggacgatcc gctcgggccg atcctccggg
     6121 cggcggcgtg gctggcgggt ctcaccgtgg ccttcgcggc gctcgcacgg ctgtccgtgc
     6181 ggcgtttgct cgtctgacgg ttccgatttg accgcccgtc agccgcggtg agagggtcgc
     6241 tgcgtgtgct gcctcttcct gctcgccttc ggcatcgggc cgcgcgtcgc gctcttcttc
     6301 gtctggatct tcggcgaccg cgtgcaggac gcgttcgact cgttcgcctg gccgctgctc
     6361 ggactgctgt tcctgccgtg gacgacgctg ctctacgtca tcgtctggca gtcgggcgga
     6421 ctgagcggct ggggctggtt cgtcgtcgga ctcggcgtgc tgctcgatct ggggtcgtac
     6481 ctgggccggc tcggccaccg gcgcgccggc ttctagccga tccgcaggcg gggccggtag
     6541 cgcggcgggc cggtgaggga agacctgcgt cgccggccgg caacggccga agccgtcctc
     6601 cgcggccacg tggcccgctc gttcgcaccg gcgctcgccg cgtgggaatg gactcgttct
     6661 cgatctacat catcggccgc tcggcgctct cgccggcggc gtcgtgtacc agctcgcgcg
     6721 ccgctgatca gactcgcagc cgcttcgtgg gcgccgctgc gggcggccaa acggcgacct
     6781 gatcgacgga cgcccgagac accgtgctgc ggcgcgtgta cgctccgtgt gtgagctcgc
     6841 tcgtcgttcg ggtgagatga gcgtcgccgg tcttgctacc ggccgggatc gcctcctacg
     6901 ggagctgctg gagctcatct ccgaacgcct ccctcccgac cgcgccgatc tcgtcgggcg
     6961 cttcgccacg ctgtacgggc gccgactgcc ggccgaggcc gcggagacgc tgacggccga
     7021 cgagctcttc gggcaggtgc tcggcgcgtt cgagctcgcc gacaaccgag gagtcgagcc
     7081 gatcgccgtc cgcgtcttca accccacgct cgccggcgac ggctacacga ccgtcggctc
     7141 ggtgatcgag acgaacacgc cggactcgcc cttcctcgtc gactccgtgt cggaggagct
     7201 cgacgctcgc gagctcggga tccgacttct cctgcatccc gtcctcggca tcgagcgaga
     7261 cgacgcaggc cgcatcgccg gcgttacctc cgcgctgcag gccgagtcgc tcgagtccgt
     7321 catgcacttc gaggtcgacc ggcacctggc cgcgcccgag ctcagcgatc tcgccgccgc
     7381 gaccctccgg atccttcggg acgtccagcg cgcggtcggc gacttcgagc cgatgcgggc
     7441 gaaggtcgcg acgatgatcg aggcggccgc gggaagcggc gcgcgctacg agcaggacga
     7501 gatcgatgag accgtcgcct ttctggagtg gctggacgac gacaacttcg tgtacgtggg
     7561 ctacagggag tacgagatcg tgggcgaggg ggccgagcgg gcgattcgag tgaagcccgg
     7621 cagcgggctg ggagtgctca gcgacgaggc gacgtcgacg ttcgcggaac cggtcctcct
     7681 gagcaagatc gacgacgacc tccgtcctcg cgtcgagggc ggcg
//
LOCUS       DBNGNK010000306         3890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_913706,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000306 DBNGNK010000000
VERSION     DBNGNK010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_913706"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="AC150V_09025"
     CDS             complement(<1..1382)
                     /locus_tag="AC150V_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019040.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HIR3763099.1"
                     /translation="MSDDPQAREARAEIQALRCADCARSDSADPDARPVDMLPLPVDA
                     LAGFPAGVPRVRGTNRREFLRNGVLGFAAVYAAHALNWTSVFETALAEGASDPSNQLV
                     MLYLNGGLDGLNCVVPATSANYNAYRAARPTLYRELNAPSVPGRVGSTAVPGTGGELS
                     FANLVVSGAGNNGDAKGLDTLWGDGSGGAGSDVAYLPGMHFLPANGSHFEATDYIFAG
                     NLARMSTGWLGRWLDLYGSTTNPLQAVSIGSSLSKTIRTATAPVCSLPTAFGNFRFSV
                     SGVSSSQANVNDEVGRISAIPASTAEVAVSRSVFQTTVNVSNQLGSTSPPGDNPDYPD
                     SSLSDRLKTAATLLSANLGVKIVTIDWGSFDTHGDQATAMDPQITTLSRALAAFKNDL
                     AARGIEQRVTTVVFTEFGRRVGENGSVGTDHGAGGPCFVMGSAVRGGLAAGHPSVTSL
                     MNGNLGVTTD"
     gene            complement(1398..2714)
                     /locus_tag="AC150V_09030"
     CDS             complement(1398..2714)
                     /locus_tag="AC150V_09030"
                     /inference="COORDINATES: protein motif:HMM:NF020390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HIR3763100.1"
                     /translation="MLTLEMVDRLFWRAGFGASQADRARWSGRPLDDAVDWLLGTRGG
                     YVAPQATLGGQPIDRLAGTTDLVLDWVGRMVSAANPLVERLTFFWHRHWACSRADVDH
                     VFMVRQNDLFRRYSDLATNPSADFRSLAYEVGEDPAMLRFLNGELNRRGRPNENYARE
                     LMELFCLGVTDDSGNPNYTETDVRELSRALSGWTIDTSDSLNPRGVFNSSRFDNGSKT
                     FLGATGNFGARQAVDVVLDKPSHPSFLVRKLWSEFISGPPDAATLADLTSTYTGSGFR
                     IEPLLRKILMHPQLLASLAEPTLVKPPIVYVAGGFRQVAMNVQDSRPYSRLREMGQLP
                     FFPPNVSGWEYGTAFLTTNAALSRWDFASDLAGRISLTDVPGESPQAAFDRALASSGQ
                     PWLSAGTRTAMLDYASRAPANNSSNRRARQRVLMTMALGGPDAHVM"
     gene            complement(2915..>3890)
                     /locus_tag="AC150V_09035"
     CDS             complement(2915..>3890)
                     /locus_tag="AC150V_09035"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 26 protein"
                     /protein_id="HIR3763101.1"
                     /translation="MLALGLFAAVALPAAESKAATVAKKPGAAAKRAAAKPPALPKRC
                     TELGIYRDNPVAAFPSLTRGFGSHITTVSTYVTAGSALDPKLAALARARGLRLLVTWM
                     PDAGADDPDLPRFSLKYIIGGSLDADLRALARELKAARVPVLFRPMPEPNTPWYSWSG
                     LANGNTPSEYALAWKHVRKVVRKIAGPRVSFVWSPYARSIPDIPANSIERYFPGTRFV
                     DVVGASGYNFGAVRGLEWLQAKALFMPAYTAIQKLARKPFWITETGSTAAGGSKTRWI
                     ASLRALRTAMPKLRGLVWFDVKDPEGDFRLVGPALKTARSLLKGRCVV"
BASE COUNT          593 a         1328 c         1392 g          577 t
ORIGIN      
        1 aagtcggtcg tgacgcccaa gttcccgttc atcagcgacg tcacgcttgg atggccggcg
       61 gcgaggccgc ctcggacggc cgagcccatc acgaagcacg gcccgccggc gccgtggtcg
      121 gtgccgaccg agccgttctc gccgacgcgc cggccgaact cggtgaagac gacggtggtc
      181 acgcgctgct cgatgccgcg ggcagcgaga tcgttcttga acgccgccag cgcgcgtgag
      241 agcgtcgtga tctgcggatc catcgccgtg gcctggtcgc cgtgcgtgtc gaaggagccc
      301 cagtcgatgg tgacgatctt gacgccgagg ttggcagaga gcagcgtcgc agccgtcttc
      361 aaccgatcgg acagggagct gtccgggtag tcgggattgt cacccggcgg gctcgtcgag
      421 ccgagctggt tggagacgtt caccgtcgtc tggaacaccg acctgctcac cgctacctcg
      481 gccgtcgacg ccggaatcgc cgagatccgc cccacctcgt cgttgacgtt cgcctgcgac
      541 gagctgaccc cgctcacgga gaagcggaag ttcccgaagg ccgtcggcag agagcagacc
      601 ggagccgtcg cagtgcgaat cgtcttggag agcgaggagc cgatcgagac cgcctgcagc
      661 gggttcgtcg tggagccgta gaggtcgagc cagcgaccca gccagccggt gctcatgcgc
      721 gcaaggttgc cggcgaagat gtagtcggtc gcctcgaagt gcgatccgtt cgcgggaagg
      781 aagtgcatgc ccggcaggta ggcgacgtcg gagccggcgc cgccgctgcc gtccccccac
      841 agggtgtcga gccccttggc gtcgccgttg ttcccggccc ccgagacgac cagattggcg
      901 aacgacagct ctcccccggt gccgggcacc gcggtcgagc cgactcgccc cgggacggag
      961 ggcgcgttca gctcccggta cagggtgggt cgggccgcgc ggtaggcgtt gtagttcgcc
     1021 gacgtcgccg gcacgacgca gttgagcccg tccaggccgc cgttcaggta cagcatcacc
     1081 agctggttgg acgggtctga ggctccctcg gccaacgccg tctcgaagac gctcgtccag
     1141 ttgagggcgt gcgcggcgta gacggccgcg aagccgagca cgccgttgcg caggaactcc
     1201 cgccggttgg taccgcgcac gcgcggcacc ccggcgggga agcccgcgag ggcgtcgacc
     1261 ggcagcggca gcatgtcgac cggacgggcg tcaggatcgg cgctgtccga ccgggcgcag
     1321 tctgcgcaac gaagtgcctg gatctcggcc ctggcctctc gggcctgcgg gtcgtcgctc
     1381 atcgagcccc tctctcgtca catgacatgg gcatcgggac cgccgagggc catcgtcatc
     1441 agcacccgct gtcgtgcgcg gcggttggag ctgttgtttg caggggcgcg gctcgcgtag
     1501 tcgagcatgg cggtcctcgt gccggcgctt agccacggct ggccgctgct ggcgagagcg
     1561 cggtcgaacg cggcctgagg ggattcgccg ggaacgtcgg tcaggctgat ccggcctgcg
     1621 aggtcggagg cgaaatccca gcgggagagc gcggcgttcg tcgtcaggaa ggccgtcccg
     1681 tactcccagc cggagacgtt gggcgggaag aacggcaact gccccatctc ccggagccgc
     1741 gagtacggcc gcgaatcctg cacgttcatc gcgacctgcc ggaagccccc ggccacgtag
     1801 acgatcggag gcttgacgag cgtgggctcg gcgagcgagg cgagcagctg cgggtgcatc
     1861 aggatcttcc gcagaagcgg ctcgatccgg aagccgctgc cggtgtaggt cgacgtcagg
     1921 tccgcgagcg tcgccgcgtc cggaggcccc gagatgaact cggaccagag ctttcgcacc
     1981 aggaacgaag ggtggctcgg cttgtccagg acgacgtcca ccgcctgccg agcgccgaag
     2041 tttcccgtcg cgccgagaaa cgtcttcgac ccgttgtcga aacggctcga attgaacact
     2101 ccacgggggt tgagcgagtc gctcgtgtcg atcgtccagc ccgagagcgc ccgcgacagc
     2161 tcgcgtacgt cggtctccgt gtagttcggg ttgccggagt cgtcggtgac gccgaggcag
     2221 aagagctcca tcagctcccg cgcgtagttc tcgttcggcc ggccccgcct gttgagctct
     2281 ccattgagga agcggagcat ggccgggtcc tcgccgacct catatgccag gctccggaag
     2341 tcggcgctcg ggttcgtggc caggtcggaa tagcgtcgga acaggtcgtt ctgacgcacc
     2401 atgaacacat ggtcgacgtc ggcgcgcgag caggcccagt ggcggtgcca gaagaacgtg
     2461 aggcgctcca caaggggatt cgccgccgac accatgcgac cgacccagtc gaggacgagg
     2521 tcggtcgtcc ctgcgagccg gtcgatcggc tggccgccga gcgtcgcctg cggcgcgacg
     2581 tatccgcccc gtgtgccgag gagccagtcg acggcgtcgt ccagagggcg tccggaccac
     2641 ctcgcgcggt cggcctggct ggcgccgaag cccgctcgcc agaagagccg atcgaccatc
     2701 tcgagcgtga gcacgggccc gacgggcgcc ccgccgccgc cactccctcc gccgccaccg
     2761 ctgcttcctc cgccactgcc gcctccccct ccaccgccgc cgacgctgat cggcgggggt
     2821 gcgggcggcg cggcgggtgc aggctccgga ggcggcggga ccgtgagtcg cttccgccgc
     2881 ctgacgacgc ggcgcttcgg cgccccgacc gccatcagac gacacacctc cccttcagca
     2941 gcgaacgagc cgtcttcagc gccggcccga cgagccggaa gtccccctcc gggtctttca
     3001 cgtcgaacca gacgagcccg cgcagcttcg gcatcgcggt gcgcagcgcg cgcaaggagg
     3061 cgatccaacg ggtcttgctg ccgcccgctg ccgtcgagcc cgtctcggtg atccagaacg
     3121 gcttcctggc cagtttctgg atggcggtgt acgcgggcat gaagagcgcc tttgcctgga
     3181 gccactcgag cccacggacc gctccgaagt tgtagccgga agcgcccacg acgtcgacga
     3241 agcgcgtgcc ggggaagtag cgctcgatcg agttcgccgg gatgtccgga atgctccgcg
     3301 cgtacggact ccaaacgaag ctgacccgcg gaccggcgat cttccgcacg accttgcgca
     3361 cgtgcttcca cgcgagtgcg tattccgacg gggtgttccc gttcgcgagg cccgaccacg
     3421 agtaccacgg ggtgttcggc tccggcatcg gccggaagag cacgggcact cgggcggcct
     3481 tcagctcgcg cgcgagcgct cgcaggtcgg cgtcgagcga gccgccgatg atgtacttga
     3541 gcgagaagcg gggcaggtcg ggatcgtccg caccggcgtc gggcatccag gtcacgagca
     3601 ggcggagacc gcgggcgcgc gcgagcgccg cgagcttcgg atcgagcgcg ctgcccgctg
     3661 tgacgtatgt ggagacggtc gtgatgtggg agccgaagcc cctcgtcagc gacgggaagg
     3721 ctgcaaccgg gttgtcacgg tagatcccga gctcggtgca gcgcttcggc agcgccggcg
     3781 gcttggcggc agcccgcttc gcggccgcac ccggcttctt ggccaccgtc gcggccttgg
     3841 actcggcggc tggaagtgcg acggcggcga agagccccag cgcgagcatc
//
LOCUS       DBNGNK010000307         4197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1631261,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000307 DBNGNK010000000
VERSION     DBNGNK010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1631261"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1075
                     /locus_tag="AC150V_09040"
     CDS             11..1075
                     /locus_tag="AC150V_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR3763102.1"
                     /translation="MTELSIERGLRRVIGDGADVPDREVAGLDERDLLALYRSLVLLR
                     TYDERSVVYHRQGRIGTYAIFWNHEAMQAGSVHALADDDWLFPSYRESAIGILRGIPV
                     STILSWWRGHPTGWWNPADFGVASICVPIGTHVPHAAGFAWGSRLRGAGSVAIAYFGD
                     GATSEGAFHEGVNLAAVMRAPLILFCNNNQWAISTPLSAQTAAETLADKALGYGIPAI
                     RVDGADVLAVFEATAEAAERARAGGGPTFIEAVTYRAAPHGTADDPSSYVDAERVARQ
                     RRDECVGRFAGWLERAGLLSEELAAAVKEEALETMREGIVRAEAELPPDPQLVFEHAY
                     ADPPPSLARDLAELEGVIGR"
     gene            1072..2064
                     /locus_tag="AC150V_09045"
     CDS             1072..2064
                     /locus_tag="AC150V_09045"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3763103.1"
                     /translation="MTVVTATRELTLVDAVNDALHVELERDPSVMVIGEDVGRAGGVF
                     RATAGLLDRFGPDRCVDTPLAEAGILGSAVGLCLAGWRPVCEMQYDAFSYPCLDQLIT
                     HVGRYRWRTRGRMEFPITVRMPYGGGVRAPELHDDSPEAYYVHTPGVKVAIPATPADA
                     KGLLAAAIRDPDPVVVLEPKLVYRTARGPVPEGEHVVPLGKARVARKGSDLTLVAYGA
                     MVPVAQRAAAALAGEASVEVLDLRTLKPLDEDALLASAGKTGRVVLVQEAPRTCGYAA
                     ELAALLAEKAILDLRGPVLRVTGYDVPYPYWQLEDYYMPSVDRVLHAARRLLTF"
     gene            2075..3172
                     /locus_tag="AC150V_09050"
     CDS             2075..3172
                     /locus_tag="AC150V_09050"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3763104.1"
                     /translation="MAYEFRLPDLGEGLTEGEVARWLVGEGDVIAEDDPLVEIQTDKA
                     TVEIPSPAAGTVLKILVDEGGVAAVGSVLVVIGEEGETLLRPVAGPTVDEPKPPPITA
                     VESPAARRIAATPRVRRLADEHGVDLATLSGSGHGGRISEEDVLRAAARGESAEGRAV
                     PVRGIRRRIVEHLATSHREIPAVTFVEECDFTGVELARIVPLTLRATALALRDYPELN
                     ARLEGDEIRYLDRYDLGFAVQTGEGVLVPVVRSCASTGLDELAAEVERLADAARSGSL
                     TPDELRGSTFTVTSAGKLGGLFVTPLINHPEVGILGIHRIAQRPVVRDSEIVVRDIGH
                     VSVTFDHRVVDGARAAAFCLDVIGRLQAGEA"
     gene            3210..3641
                     /locus_tag="AC150V_09055"
     CDS             3210..3641
                     /locus_tag="AC150V_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763105.1"
                     /translation="MGDWYVIGIALGTGVAFGVLAAALLAWIKLGWAASTAVAVAAGL
                     VAGFVTNRWIGPEWVGPVAGVAGGLIGSVSGSVVARGAIRRGGTPGGLAFLLGSFSLM
                     IFLLALVPAVGFVVAVIVPIAAFRNYGRDTGKHAGLRTLAK"
     gene            3638..>4197
                     /locus_tag="AC150V_09060"
     CDS             3638..>4197
                     /locus_tag="AC150V_09060"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR3763106.1"
                     /translation="MTPRKLILVVIDGLSATTFEDAIDDGSLPTLGRLAEHGTYRRAV
                     STFPSLTPVCLSSIATGAHPDIHGIPHLVWYHRDERRIVEYGSSFGAMRAAGLGRSIR
                     DTLVNMNDAHLSPNAVTVFEALEDAGLVPAAVNFTAYRGRTEHHSTLPLFGGTVRAPK
                     RFFFYNLFESDATGAPLSWRNRKDGT"
BASE COUNT          591 a         1469 c         1467 g          670 t
ORIGIN      
        1 ccattaaggg gtgaccgagc tctcgatcga gcgcggactg cgacgcgtga tcggtgacgg
       61 cgccgacgtg ccggatcgcg aagtcgccgg cctcgacgag cgtgacctgc tcgcgctcta
      121 ccgctcgctc gtgctgcttc gtacatacga cgagcgctcc gtcgtctacc accggcaggg
      181 ccggatcggg acgtacgcca tcttctggaa ccacgaggcg atgcaggcgg ggtcggtgca
      241 cgccctcgcc gacgacgact ggctcttccc cagctaccgc gagtcggcga tcgggatcct
      301 ccgcggcatt cccgtctcca cgatcctctc ctggtggcgc gggcacccga ccggctggtg
      361 gaatcccgcc gacttcgggg tcgcctcgat ctgtgtcccg atcgggacgc acgttccgca
      421 cgcggcaggt ttcgcgtggg ggtcccggct ccggggtgcg ggcagcgtcg cgatcgccta
      481 cttcggcgac ggtgccacct ccgaaggtgc gttccacgaa ggcgtgaatc tcgctgccgt
      541 gatgcgggcg ccgctgatcc tcttttgcaa caacaaccag tgggcgatct cgaccccgct
      601 ctccgcccag acggcggcgg agacgttggc cgacaaggca ctcggctacg gcatccccgc
      661 gatccgcgtg gacggtgccg acgtcctcgc ggtcttcgag gcgaccgccg aggccgcgga
      721 gcgggcacgt gcgggcggcg ggcccacgtt cattgaagcc gtgacgtacc gcgcggcgcc
      781 gcacgggacg gcggacgacc cgagctccta cgtcgacgcc gaacgcgtcg cgcggcaacg
      841 gcgcgacgaa tgcgtcggac ggtttgcagg ctggctcgag cgggctgggc tgctcagcga
      901 ggagctcgcc gcggccgtga aggaggaagc gctcgagacg atgcgagagg gaatcgttcg
      961 tgcggaggcc gagctgcctc ccgacccgca gctcgtcttc gagcacgcct acgccgaccc
     1021 gcctccgtct cttgcccggg atctcgccga gctggagggg gtgatcggtc ggtgacggtc
     1081 gtcacggcga cccgtgagct cacgctcgtg gatgcggtca acgacgccct ccacgtcgag
     1141 ctcgaacgcg acccgtccgt gatggtgatc ggcgaggacg tcggtcgtgc cggcggcgtc
     1201 ttccgagcga cggccggtct cctcgaccgc ttcggccccg atcggtgcgt cgacacgccg
     1261 ctcgcggagg cggggatcct cggcagcgcg gtggggctct gcctggcggg atggcgtccg
     1321 gtctgcgaga tgcagtacga cgctttcagc tacccgtgcc tcgaccagct gatcactcac
     1381 gtgggccgct accggtggcg cacgcgcggc cggatggagt tccccatcac ggttcggatg
     1441 ccgtacgggg gcggcgtccg ggcgcccgag ctccacgacg actcgcccga ggcgtactac
     1501 gtccacactc cgggcgtgaa agtcgcgatt ccggcgactc cggcggatgc caaggggctc
     1561 cttgcggcgg cgatacgcga tccggacccg gtcgtcgtgc tcgagcccaa gctcgtctac
     1621 cggacggcac gcgggccggt tcccgaagga gagcacgtcg tcccgctcgg caaggcacgt
     1681 gtggcgcgca aggggtccga tctcacgctc gtcgcctacg gggcgatggt tcccgtcgcg
     1741 cagcgggccg ctgcagcgct cgcaggcgag gcgtcggtcg aggtcttgga ccttcgcacg
     1801 ctgaagcccc tcgacgagga cgcgttgctc gcgtccgccg gcaagacggg tcgcgtcgtc
     1861 ctcgtgcagg aggcgccgcg cacgtgcggc tacgcagccg agctcgccgc gctcctggca
     1921 gagaaggcga tcctcgacct gcgcggtccg gtgctgcgtg tcacgggcta cgacgttccc
     1981 tacccctact ggcagctcga ggactattac atgccatcgg tcgaccgcgt cctccatgcg
     2041 gcgcgaaggc tgctcacctt ctagcctccg accggtggcg tacgagttcc ggcttcccga
     2101 cctcggcgag ggcctcaccg agggcgaggt cgctcgctgg ctcgtgggcg aaggggacgt
     2161 gatcgccgag gacgatccgc tcgtcgagat ccagaccgac aaggccacgg tcgagatccc
     2221 gtcgccggcc gccgggacgg ttctcaagat cctcgtcgac gagggaggtg tggcggcggt
     2281 cgggtcggtc ctggtcgtga tcggagagga gggcgagacg ctcctgcgac ccgtcgccgg
     2341 gccgacagtc gatgagccga aacccccacc gatcactgcg gtcgagtccc cggccgctcg
     2401 ccgtatcgcc gccaccccac gcgtgcggcg ccttgcggat gagcacggcg tcgacctcgc
     2461 gactctctcg ggcagcggcc acggcggtcg gatcagcgag gaggacgtgc tccgagccgc
     2521 ggcacgaggg gagtccgccg aaggccgggc tgttccagtc agagggattc ggcgccggat
     2581 cgtcgagcac ctggcgacgt cgcaccggga gatcccggcc gtgaccttcg tcgaggaatg
     2641 cgacttcacc ggcgtcgagc ttgcgcgaat cgtaccgctg accctgcggg ccactgcact
     2701 cgctcttcgt gactaccccg agctgaacgc ccgtctcgaa ggcgacgaga tccgctacct
     2761 cgatcgctac gacctcggct tcgccgtcca gaccggcgaa ggcgtcctcg tgcccgtcgt
     2821 ccgctcctgc gcgtcgaccg ggctcgacga gttggccgcg gaggtcgagc gcctcgccga
     2881 cgcggcacgg agcgggagcc tcacgccgga cgagctacgc ggctcgacct tcaccgtcac
     2941 gagcgccggc aagctcggcg gtctgttcgt gacgccgctg atcaaccacc ccgaggtggg
     3001 catcctcggc atccaccgga ttgcgcagcg gccggtcgta cgtgacagcg agatcgtcgt
     3061 ccgggacatc ggacacgtct cagtcacctt cgaccatcgc gtcgtcgacg gtgcacgggc
     3121 cgcggccttc tgcctcgacg tgatcggtcg actgcaggcc ggcgaggcgt aggcccgccc
     3181 cgtctggggg ccactacact gcccggacga tgggtgactg gtacgtgatc ggaatcgcgc
     3241 tcggcaccgg cgtcgccttc ggcgtcctcg ccgcggcgct gctcgcgtgg atcaagctcg
     3301 gttgggcggc ctcgacggcc gtggcggtgg ccgccggact cgtggcaggc tttgtcacca
     3361 accggtggat cgggccggaa tgggtcggcc ccgtggccgg ggtcgcggga ggcctgatcg
     3421 gttcggtatc ggggagcgtc gtggcgcgcg gcgcgatccg gcgcgggggc acacccgggg
     3481 ggctggcctt cctgctcggc tcgttctcgc tgatgatctt tctgctagcg ctcgttccgg
     3541 cggtcggatt cgtcgttgcc gtgatcgtcc cgatcgctgc attccgcaat tacggacggg
     3601 acacgggcaa gcacgcgggt cttcgcacgc tggcgaagtg acgccccgca agctgatcct
     3661 cgtggtcatc gacgggctgt cggcgacgac cttcgaagac gcgatcgacg acggctcatt
     3721 gccgacgctc ggccggctcg ccgagcacgg cacgtatcgg cgcgccgtct ccaccttccc
     3781 ctcgctgacg cccgtgtgcc tttcgtcgat cgcgacaggc gcgcacccgg acatccacgg
     3841 cattccgcat ctcgtctggt accaccgcga cgaacggcga atcgtcgagt acggctcctc
     3901 ctttggcgcg atgcgagccg cagggctcgg ccgctcgatc cgcgacacgc tggtcaacat
     3961 gaacgacgcg cacctgagcc cgaacgcagt gaccgtcttc gaggcgctcg aggacgccgg
     4021 cctggtgccc gccgcggtga acttcaccgc ctaccgagga cgaacggagc atcacagcac
     4081 gctgccgctg ttcgggggga ccgtacgcgc gcccaagcgg ttcttcttct acaacctgtt
     4141 cgagtccgac gcgacaggag cgccgctctc ttggcgcaac cgcaaggacg ggacgat
//
LOCUS       DBNGNK010000308         3861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1199311,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000308 DBNGNK010000000
VERSION     DBNGNK010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1199311"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC150V_09065"
     CDS             <1..207
                     /locus_tag="AC150V_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3763107.1"
                     /translation="LERLGYVERHADPDDGRARIVKLTERGRALDAAVWRAGREVEKS
                     WRRLFGTRQWAMLNDALGKLIAAD"
     gene            300..908
                     /locus_tag="AC150V_09070"
     CDS             300..908
                     /locus_tag="AC150V_09070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR3763108.1"
                     /translation="MEFTEVLRRRGMVRNYTDEPVSRDAVERIVARARTAPSAGFSQG
                     VRMVVVTDAGTRSRIAELADEPYYVERGHEPWISRVPVHVVVAVREDDYHDRYREADK
                     LENGAEIEWPVPWWWVDAGKATMLLLLAAVDEGLGAGLFTLYPKESNDRLREHLRMPA
                     DISVVGVVAIGHPAPNPNAEKRRADLRRRRRPLENVVRWEHW"
     gene            complement(954..1349)
                     /locus_tag="AC150V_09075"
     CDS             complement(954..1349)
                     /locus_tag="AC150V_09075"
                     /inference="COORDINATES: protein motif:HMM:NF053729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP-binding domain-containing protein,
                     MshE-related"
                     /protein_id="HIR3763109.1"
                     /translation="MEAQSRSARLIGAIFVDMGVITDEQLAEALARQRTTGELIGEIV
                     VSKFGVSRLALASALAEQKAERDTRRAASEPEAEADGAVDAVGSLTSGELDAAIRSAL
                     APVRAEIEAVEACLASFRERLDALERHSA"
     gene            1589..3145
                     /locus_tag="AC150V_09080"
     CDS             1589..3145
                     /locus_tag="AC150V_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763110.1"
                     /translation="MRITGWAFACVMVAVVAGGASFAASSDTRYVPELRKSVAQVDAS
                     WREVAALDKSDQAAVAAAGKRLASAAKAACRTLRAYRDAQYRYGTDEEWQLMLQLLGR
                     LDEAGSKFGSLAAAARQAATPTQKSLAEQALRAYALRAARKQIAKWVKDERLSAILTT
                     GSLRTARQRLEKEIAARIRAEARRLSLQSVGVAISLDVDIRTQARRTAEQAAVRWLAK
                     TVLKASPNAIVVQIVGKPIVRFVATQLKRSLRNYKNVTSRTQRTLKGFDNRMAQLQRL
                     IDRPGASRLATVRRALADAQRAIDATRYLQNDLKKQGRDALLVALKRGEILLKARMRA
                     TRAVFLLDSKLARADLRKTADDVCALSREIESIGKKLPRSGKPPGAGGCTVALRADKG
                     KPGVTGQMTLVLRPNPALTNEVQAFVTISTTGAEGPKDDVPVIFKIWFYKKPGDPLIL
                     GQGDPRGLDFNLDAAAGRTTPQRVEGMFLNVSDRAVLAGSPFFVRLFVVAFDDKGSVG
                     TELEGLCRTG"
     gene            complement(3152..>3861)
                     /locus_tag="AC150V_09085"
     CDS             complement(3152..>3861)
                     /locus_tag="AC150V_09085"
                     /inference="COORDINATES: protein motif:HMM:NF039204.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5715 family protein"
                     /protein_id="HIR3763111.1"
                     /translation="IRNAGAWRLLSRLSDDSPTYYWRVLAALEIMRLHREDAELLGEL
                     AARHGAKASAEEVLHPLEQTERFEQPSDVEQAWRDGDLQPVPDEPSRLHFRIDPQMGE
                     LAGRLGKEPSLYRGLRPEALALLLYTAERVHALSGAAQPLRVTSTVRDDAYQQLLRRG
                     NREATGAYSLHTTGYAFDVLRRYESRAQAVAFQYVLDDLEARNLIAWVREPAAIHVTV
                     GPAAEALVPLLLAEERP"
BASE COUNT          646 a         1315 c         1362 g          538 t
ORIGIN      
        1 ctcgagcgcc tgggctacgt ggaacggcat gccgacccgg acgacgggcg cgcccgcatc
       61 gtcaagctga ccgagcgggg ccgagcgctc gacgcggccg tctggcgggc aggtcgggag
      121 gtcgagaagt cgtggcggcg gctcttcggc acacggcaat gggcgatgct caacgacgcg
      181 ctcgggaagc tgatcgcggc cgactgagcg gctccggcag agcctgatcg ccgccacgcg
      241 gacggctccg aaaaccggca cgtgatggaa ccctgcggac gctggataat gcgcgcgtcg
      301 tggagttcac ggaggtactt cgccgccgcg ggatggtgcg caactacacc gacgagcccg
      361 tttcacgcga cgcagtcgag cggatcgtcg cccgcgcccg cacagcgccg agcgccggct
      421 tcagccaggg cgtgcgcatg gtcgtcgtca ccgacgccgg aacccgcagt cgaatcgccg
      481 agctcgccga cgagccctac tacgtcgagc gcggccacga gccgtggatc tcccgagtgc
      541 cggtgcacgt cgtcgtcgca gtgcgcgagg acgactacca cgaccgctac cgggaggcgg
      601 acaagctcga gaatggcgct gagatcgagt ggcccgtgcc ctggtggtgg gtcgacgccg
      661 gcaaggcgac gatgctgctg ctgctcgccg cagtggacga gggtctcggc gccggcctct
      721 tcaccctcta cccgaaggag agcaacgacc ggctccgcga gcacctccgc atgcccgccg
      781 acatcagcgt cgtcggcgta gtggcgatcg gtcacccggc gccgaacccg aatgcggaga
      841 agaggagagc ggatctacgt cggcggcgaa ggcccctcga gaacgtcgtc cgctgggaac
      901 actggtagcc gagccggccg cgcagcgcat cgccgcgtag cagagcctgc tgttcaggca
      961 gagtgccgct cgagcgcgtc gagccgctcg cggaaggacg cgaggcacgc ctcgaccgcc
     1021 tcgatctcgg cacgcacggg agcgagtgct gagcggatcg cggcgtcgag ctccccggag
     1081 gtgagcgagc cgaccgcatc gacagctccg tccgcctcgg cttccggctc ggacgcagcg
     1141 cgcctagtat cccgctcggc cttctgctcg gcaagtgcgc tcgcgagcgc gagccgcgaa
     1201 acgccgaact tcgacaccac gatctcgccg atgagctcgc ccgtcgtgcg ctgtctcgcg
     1261 agcgcttcgg cgagctgctc gtcggtgatg acacccatgt cgacgaagat ggcgccgatc
     1321 aggcgagccg accgcgactg tgcttccatc cttcctctat cggcaatccc tcgtccgagg
     1381 catagggggc gccgctggcc ggccgtgcgc cgctcgcgga ttgccccatt ccgacgggta
     1441 gacgagcggc gggagagcct cgggccgtac gcccacgagg atgggcaacg gacttcacca
     1501 ggctggtctg ccgcgtactt cttccggtcg tggatcggca gatggaccgg atgcgcgggc
     1561 agccgcattg cgcaacggtg aaagagcaat gcggatcacc ggttgggcgt tcgcctgtgt
     1621 catggttgcg gtcgtcgccg gcggcgcgtc gttcgcagcg tcgtccgaca ctcgctacgt
     1681 cccagagctg cgcaagagcg tcgcacaggt ggacgcgagc tggcgcgagg tcgccgccct
     1741 cgacaagtcc gaccaggctg cagtcgcggc agccgggaag cgcctggcgt cagctgcgaa
     1801 ggcagcctgc cggacgcttc gcgcctaccg ggacgctcag taccgctacg gcaccgacga
     1861 ggagtggcag ctgatgctgc agctcctagg ccggctcgac gaggccggct cgaagttcgg
     1921 ctcgctcgcc gcagccgccc gccaggcggc aacaccgacg cagaagagcc tcgccgagca
     1981 agcgcttcgt gcgtacgccc tgcgggcggc ccgcaagcag atcgccaaat gggtcaagga
     2041 cgagcgcctg agtgcgatcc tgacgaccgg cagcctcagg accgcgaggc agcgtctcga
     2101 gaaggagatc gctgcacgga tacgggccga ggcgcgacgg ctctcgctcc agtcggtcgg
     2161 agtcgcgatc tcgctcgacg tcgacatcag gacacaggca cggcggaccg ccgagcaggc
     2221 cgcagtcagg tggcttgcca agaccgtcct caaggccagc cccaatgcga tcgtcgttca
     2281 gatcgtcggc aagccgatcg tgcgcttcgt ggccacccag ctgaagcggt cactccgcaa
     2341 ctacaagaac gtgacaagcc gcacgcaacg caccctgaaa ggcttcgaca accgtatggc
     2401 ccagctccag cggctgatcg atcgccccgg cgcgtcacgg cttgccaccg tgcggcgggc
     2461 tctggccgac gcccagcgcg cgatcgacgc cacccgctac ctgcagaacg atctgaagaa
     2521 gcaggggcgc gacgcgctgc tggtggcgct gaagcgaggc gagatcctgc tgaaggcgag
     2581 gatgcgcgcc actcgcgcgg tcttcctcct cgactcgaag ctcgcccggg ccgacctgcg
     2641 aaagaccgcc gacgacgtgt gcgcgctgag ccgcgagatc gagagcatcg gcaagaagct
     2701 gccgcggagc ggtaaaccgc ctggcgcggg agggtgcacg gtggcgttgc gagccgacaa
     2761 gggaaagcct ggcgtgaccg ggcagatgac gctcgtcctg agaccaaatc ccgcgctcac
     2821 caacgaggtg caggcgttcg tgacgatctc gacgaccggc gccgagggac cgaaggacga
     2881 cgtgccggtg atcttcaaga tctggttcta caagaagcca ggggatccgc tgatcctcgg
     2941 gcagggcgac ccgcgcggcc tcgacttcaa cctcgacgcc gcggcgggac gcacgacgcc
     3001 acagcgcgtc gagggcatgt tcctcaacgt ctcggaccgg gcagtcctcg caggctcgcc
     3061 gttcttcgtc cgcctcttcg tcgtcgcatt cgacgacaag ggaagcgtgg ggacggagct
     3121 cgagggcctc tgccggacgg gatagcgtcc cctagggcct ctcctcggcg agcagaaggg
     3181 gcacgagagc ctcggctgcg ggcccgacgg tgacgtggat cgccgccggc tcgcgcaccc
     3241 aggcgatcag gttgcgcgcc tcgaggtcgt cgagcacgta ctggaaggcg acggcctgag
     3301 cgcgggactc gtagcgacgc aggacgtcga acgcgtatcc ggtcgtgtgc agcgaatacg
     3361 cccccgtggc ctcacggttg ccgcgacgga ggagctgctg gtaggcatcg tcgcggacgg
     3421 tgctcgtcac ccgcagcggt tgcgccgctc cgctcagggc gtgcacgcgc tcggccgtgt
     3481 agaggagcag cgcgagggcc tcggggcgca gtccacggta gagagagggc tccttgccaa
     3541 gccgaccggc cagctcaccc atctgcggat cgatccggaa gtggagtcgt gacggctcgt
     3601 ccggcaccgg ctgcaggtcg ccgtcgcgcc aggcctgctc gacatcggac ggctgctcga
     3661 accgctccgt ctgctccagc ggatggagga cttcctcggc gctcgccttt gcgccgtgac
     3721 gtgcggccag ctcgccgagg agctcggcgt cctcccggtg caggcgcatg atctcgagcg
     3781 cggcgagcac gcgccagtag taggtcggcg agtcgtcaga gagtcgcgag agcagccgcc
     3841 aggcaccggc attgcggatc g
//
LOCUS       DBNGNK010000309         6970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1699131,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000309 DBNGNK010000000
VERSION     DBNGNK010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6970
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1699131"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC150V_09090"
     CDS             complement(<1..233)
                     /locus_tag="AC150V_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763112.1"
                     /translation="MPGRRWEMPTRDELPERPADPAVRQANLHRILRLFRPYRRLLSV
                     VFFLIVVAAVLGAIPAFLLREVLDVAIPEENVRL"
     gene            complement(254..796)
                     /locus_tag="AC150V_09095"
     CDS             complement(254..796)
                     /locus_tag="AC150V_09095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR3763113.1"
                     /translation="MRRAYRLEVRGQERVPSAGPVIVAANHESVLDPVVLGVAFPRVL
                     HFLAKEELWRVRPLTPLLDALGGIPVARGRGDLGALSRALDVLAAGHAVALFPAGGVR
                     RQGPWLRGAARLALASGAPLVPVRLLGTAAALSPGRVGFPRMAALIGEPIPVEQARPT
                     IAAAKDLTQRLRVAVASLTQ"
     gene            complement(832..1494)
                     /locus_tag="AC150V_09100"
     CDS             complement(832..1494)
                     /locus_tag="AC150V_09100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR3763114.1"
                     /translation="MRRLFPLPTGADVRIRNEAGVLAVVLVNGGTARAIPGTWSATSE
                     LLADELAPRHPDILFVEVRYRIKSWNELPSCMADGSAAVDLVLAEGVRTCMLVGFSMG
                     GAVSVGVAEHDGVSSVLGLAPWFPERLELDGLRGKRLDVIHGAWDRWLPGIPGVSPSS
                     SRVGFERALELGAEGTYTLVPRGLHGAALRSRSGRLVRLPGWRRWVELTGEALDRFRV
                     AA"
     gene            complement(1491..2081)
                     /locus_tag="AC150V_09105"
     CDS             complement(1491..2081)
                     /locus_tag="AC150V_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR3763115.1"
                     /translation="MRFAFFSLAVVAAGVAVYVLRGGDSAPRVSAGAVSLVGDSLNVG
                     IERYLPDELTGWRIQNHNEVGRATWQGVELVREQRERLARRLVVSLGTNDPAGDASGF
                     ATNVREVLAAAGPDRCVVWVTIHREGHAAFNDALRREAEQRRNLFLVDWAAMVRDHPE
                     WLAGDGIHATEAGYAKRAAAVAQAVEACRLVRVEPA"
     gene            2137..2970
                     /locus_tag="AC150V_09110"
     CDS             2137..2970
                     /locus_tag="AC150V_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR3763116.1"
                     /translation="MDTATSIDRFLASPSLSNATRRAYASDLRDFASWLRANGLRVGE
                     VDTRALAEYTSELGRGRRGLAPATIARRLSAVRSFLRFTLGPARVPEGAFAPRSRRRL
                     PDAPKLDEVERLLDGLRGEEPLARRNLALAELAYSCGLRSAEIVGLDLGDVDFEREAL
                     HVRGKGGKERVVPLGEAAAHALVVYLRDARPKLASGAEDALFLSARGRRLDTSVLRRL
                     VSNPHRLRHAYATHLLEGGADLRTIQELLGHSSLSTTQIYSHVDARRLRRVYDRSHPR
                     S"
     gene            3022..3933
                     /locus_tag="AC150V_09115"
     CDS             3022..3933
                     /locus_tag="AC150V_09115"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HIR3763117.1"
                     /translation="MSTSDPDVESYLLLLAARRSPRTVDAYRRDLRLLAQSRDGPVGD
                     ATPQELERWLAEMRAAGLAPSTVARRLAAARAYFRHQVLIGDRNDNPASELKPPRRPK
                     SLPRTLSPSESERLIEAAAGVTPRAMRDRSLVELLYGAGLRVSEAVGLSRSAVDLDRR
                     VVRVVGKGDKERVVPLGRPSAEALRRYLALGRPHLDRRHRPDLFLNARGGALTRAGAF
                     LILRRLAGKAGLEPERVHPHLLRHSFATHLLEGGADLRSVQEMLGHADLSTTERYTHV
                     SDKRRRDTYFRAHPHARRGPRRDEPRA"
     gene            complement(4400..5398)
                     /locus_tag="AC150V_09120"
     CDS             complement(4400..5398)
                     /locus_tag="AC150V_09120"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HIR3763118.1"
                     /translation="MVGCIGGGQLGRMLALAGINLGVSFRFLDPSPDACAGAVGELLV
                     GAYDDPALLDRLSAGAAAVTFEFENVPVDAARRVGAVPGPRSLEQGQDRLREKQLFRR
                     LGIPTARFGELADVGLPALVKKRRLGYDGKGQRLVEAAETLGPDELAEELVDFDRELS
                     IIAVRGRGGETDFYPLAENVHQSGILRVSRAPAAVGLQSEAEEIARRLLDALEHVGVL
                     AVELFEVGGRLLANEFAPRVHNSGHWTIEGSRTSQFENHLRAVLGLPLGSTETPEPCA
                     MVNLIGSLPPLPELLTLPEAHVHVYGKAALPGRKLGHVTLVGERAASAERVLALCR"
     gene            complement(5401..5865)
                     /gene="purE"
                     /locus_tag="AC150V_09125"
     CDS             complement(5401..5865)
                     /gene="purE"
                     /locus_tag="AC150V_09125"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR3763119.1"
                     /translation="MGSRSDWETMRHAADTLEQMGVPYERRVVSAHRTPDLLFEYAAG
                     AEARGLQVLIAGAGGAAHLPGMAASKSVLPVIGVPVESKALKGVDSLLSIVQMPAGVP
                     VGTVAIGRAGAVNAALLAAAILGRGDAGIRERLERFRAEQTQAVLDRPDPAG"
     gene            complement(5903..6400)
                     /locus_tag="AC150V_09130"
     CDS             complement(5903..6400)
                     /locus_tag="AC150V_09130"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR3763120.1"
                     /translation="MELHEAAALADEYAQRGLERELAALRAEWADEIEAAARNPDYRI
                     RGQAYRALAQFRFRQKLELLRRGLEDESPAARGSALIALESLSREHPGDVNQYRPQLH
                     ELAAHDSNVAVRRLAIVSLRNGSPQRDSIQLLDGIAENDDEGADVRKTARSVALFLTK
                     KSRTK"
     gene            complement(6400..>6970)
                     /locus_tag="AC150V_09135"
     CDS             complement(6400..>6970)
                     /locus_tag="AC150V_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR3763121.1"
                     /translation="TAEHGMNASTFTARVVASTGADCAACLSSAVGALSGPLHGGAPA
                     RVLPMLDAAAAADDVDSFVKDLLDSGERLMGFGHRVYRAEDPRARLLRRTAKELGSPR
                     FDVAEELERVALAELHARKPDRVLATNVEFWSAVVLDVAEVPPKLAPAMFGCSRTAGW
                     SAHIIEQKKLSRLVRPSARYVGPGSRPLP"
BASE COUNT         1110 a         2543 c         2378 g          939 t
ORIGIN      
        1 agcctgacgt tctcctccgg gatggcgacg tccaggacct cccggagcaa gaacgccggg
       61 atcgcaccaa gaacggcagc cacgacgatg aggaagaaga cgaccgagag caggcgccgg
      121 tacggacgga agaggcggag gatccggtgc aggttcgcct ggcgcaccgc cgggtcggcg
      181 gggcgctccg gcaactcgtc gcgcgtcggc atctcccagc gtcgcccggg cacccgaaca
      241 tggtgcctgt cgtctactgg gtaagcgaag cgacggcaac ccgcaggcgc tgggtgagat
      301 ccttggcggc ggcgatcgtc ggacgcgcct gctcgaccgg gatgggctcg ccgatgaggg
      361 ccgccatgcg gggaaaaccg acgcgtccag gcgacagcgc agcagccgtg ccgagcagcc
      421 gcacgggcac gagcggcgcg cccgaggcga gcgcgagccg ggccgcaccc cgcaaccagg
      481 gcccctgcct gcggacgccc cctgccggaa agagcgccac cgcgtgaccc gcggcgagga
      541 cgtccagcgc ccggctgagc gcacccagat cacctctgcc gcgcgcgacc ggtatccccc
      601 cgagcgcgtc gagcagcggc gtcagcgggc gcacgcgcca tagctcctcc ttggcgagga
      661 agtgcagcac gcgggggaac gcgacgccga gcacgacggg gtcgagcacc gactcgtggt
      721 ttgccgcgac gatgacgggc ccggcgctcg gaacccgctc ctggcctcgt acctcgagcc
      781 tgtaggcgcg gcgcacgatc ggcccgcaga gcgctcggag cgccgtatag gtcaggcggc
      841 cacccgaaac cggtcgagtg cctcgccggt cagttcgacc cagcgacgcc agccgggcag
      901 acggacgagc cgccccgatc tcgatcgcag ggccgcaccg tgcaggcccc gtggaacgag
      961 cgtgtacgtg ccctcggcgc cgagctcgag cgctcgttcg aaccccaccc gcgagctgct
     1021 cggactcaca ccggggatcc cgggcaacca gcggtcccag gcgccgtgga tgacgtcgag
     1081 ccgcttgcct ctcaacccgt cgagttccag ccgctcggga aaccaggggg caaggcccag
     1141 caccgacgac actccatcgt gctccgcgac gccgacggag acggctcccc ccatcgagaa
     1201 gcccaccagc atgcaggttc gaacgccttc ggcgaggacg aggtcgaccg ccgcggagcc
     1261 gtccgccatg cacgacggca gctcgttcca gctcttgatc cggtaccgca cctcgacgaa
     1321 gaggatgtcc gggtgtcgcg gcgcgagctc gtcggcgagc agctcgctcg ttgcgctcca
     1381 ggtgccgggg atggctcgcg cggttccacc gttgacaagg acgactgcca gcacacccgc
     1441 ctcgttgcgg atgcgtacgt cggcacccgt cggaagaggg aacagccgtc tcatgccggc
     1501 tcgacccgga cgagacgaca cgcctcgacg gcctgcgcga cggcggccgc acgcttcgcg
     1561 tagcccgcct cggtggcatg gattccgtcg ccggcgagcc actccggatg gtcacgcacc
     1621 atcgccgccc agtcgacgag gaagagattg cggcgctgct ccgcctcgcg gcgcagcgcg
     1681 tcgttgaacg cggcgtgtcc ttccctgtga atcgtcaccc atacgacaca gcgatccggg
     1741 cccgcggcgg cgagcacctc tcggacgttc gtcgcaaacc cagacgcatc tccggccggg
     1801 tcgttcgtcc cgaggctgac aacgagcctc cgggccaggc gctctcgctg ctcacgcacg
     1861 agctcgaccc cctgccacgt tgcacgcccg acctcgttgt ggttctggat gcgccagccc
     1921 gtcagctcgt cgggaaggta gcgctcgatc ccgacattga gagaatcccc gacgagcgag
     1981 accgcccccg ccgagaccct cggggctgaa tcgccacccc gcaggacgta gacggcgacc
     2041 ccggcggcga ccacggcgag agagaagaac gcaaagcgca ccgtggcatt gtggctggcg
     2101 gcaggaacgg cacgcggccg tcacgaacga cgccgtgtgg acaccgccac ctcgatcgac
     2161 cggttccttg cgagcccgtc gctcagcaac gccactcggc gtgcctacgc gtcggacctg
     2221 cgcgacttcg cgtcttggct ccgtgcgaat ggtctccgcg tcggcgaggt cgacacgcgt
     2281 gcgctcgccg agtacacgtc cgagctcggt cgcggccgtc gaggcctcgc cccggccacg
     2341 atcgcccggc gactctccgc tgtccgctcg ttcctgcgct ttacgctcgg gccggcgcgc
     2401 gtgccggagg gtgcgttcgc accgcgctcg cgtcgccggc tgccggacgc gccgaagctc
     2461 gacgaggtcg agcgtctgct cgacggcctt cgcggagaag agccactcgc acgtcgaaac
     2521 ctcgccctcg ccgagctcgc gtactcctgc ggactgcgat cggccgagat cgtgggactc
     2581 gacctcggtg acgtcgactt cgagcgcgag gcgctccacg tacgcggcaa ggggggcaag
     2641 gagcgggtcg tgcctctggg agaagctgca gcgcacgctc tcgtcgtcta ccttcgagac
     2701 gccagaccaa aactcgcgag cggcgccgaa gacgcccttt ttctctcggc ccgcggccgc
     2761 cgcctcgaca cgagtgtgct tcgccgactc gtctcgaacc cccaccggct ccggcacgcg
     2821 tatgcgacac acctgctcga gggaggagcc gacctgcgca cgatccaaga gctgctgggc
     2881 cattcctccc tctcgacgac ccagatctac agccacgtcg acgcgcgccg gctccgccgc
     2941 gtttacgacc gctcccaccc tcgttcgtga cacgagcaca agaggagcac gccgcgggcc
     3001 gccgaaacgg ttcaggcggc tgtgagcact agcgaccccg acgtcgagag ctacctcctc
     3061 ctgctggcgg cccggcgctc gccccgaacg gtcgatgcgt accggcgcga cctgcgtctg
     3121 ctcgcgcagt cgcgcgacgg gccggtcgga gacgccacgc cacaggagct cgagcgctgg
     3181 ctggccgaga tgcgcgccgc cgggctcgct ccctccacgg tcgcgcggcg actcgcggcc
     3241 gcgcgagcgt acttccggca tcaggtgctg atcggcgacc gcaacgacaa tcccgcctcc
     3301 gagctgaagc cgccgcgacg gccgaagtcc ctgccgagaa ccctctcccc ttccgagtcc
     3361 gaacgcctga tcgaggccgc tgccggcgtc accccccgtg cgatgcgcga ccgttcgctc
     3421 gtcgagctgc tctacggggc cgggctccgc gtctccgagg ccgtcggtct gagcaggtcg
     3481 gccgtggatc tcgatcgccg cgtggtgcgc gtcgtcggca agggcgacaa ggagcgagtc
     3541 gtgccgctgg gcaggccgag cgcggaggcg ctccgcagat acctggcact cggccggcct
     3601 cacctcgacc ggcggcaccg tcccgacctc ttcctgaacg cgcgtggcgg tgccctgacg
     3661 agggccggcg cgttcctgat cctccggcgg cttgcgggga aggcggggct cgagcccgag
     3721 cgggtgcatc ctcacctcct ccgccattcc ttcgcgacgc atctgctgga gggcggcgcc
     3781 gacctccgta gcgtccagga gatgctcggc cacgccgacc tgtcgaccac cgagcgttac
     3841 acccacgtct cggacaagcg tcggcgggac acctacttcc gcgctcatcc gcacgcgcgc
     3901 cgtggcccgc ggcgggacga accccgcgca tagactcgtc gaaaatacgg ttcgccgacg
     3961 aggaggtctt ccgcgagcaa gacacagacg cacggccagg gaattctggc ggcggagaac
     4021 gctgccaaac gcgaggagat catcgaggcg ctccggggcg acatccagga agagctcttg
     4081 cacgcccagc agttcgcgcg ccggatcaaa gaactctacg gcgtcgtccc cggctcgctc
     4141 gacttcgagg cccagcagac ctccctgcag ccgcccgagc tccagaccga catcgtgtac
     4201 gtgatccgcg gcgtgatcga ggccgagacc ggagcaatcg cccactacaa ccgcgtcatc
     4261 gaggctaccg acggccgtcg actgggtgac gcaggacatg gtcatcgcga tccttcaaga
     4321 cgaggaagga catcggcggc tcctcgaggg cttcctccgc gagtacgagg tcgagggcac
     4381 tgcctagcgg cagaaccgac tagcggcaga gcgcgagcac gcgttccgcc gacgccgcgc
     4441 gctccccgac gagcgtcacg tggccgagct tgcgcccggg aagagcagcc ttcccgtaga
     4501 cgtggacatg cgcctccggg agcgtgagca gctcggggag cggcggcagt gagccgatca
     4561 ggttcaccat cgcgcacggc tcgggcgtct ccgtggagcc gagcggcagt ccgagcacgg
     4621 cgcgcaggtg gttctcgaac tggctcgtgc gcgacccctc gatcgtccag tggccactgt
     4681 tgtgcacgcg gggggcgaac tcgttcgcga gcagccgccc accgacctcg aagagctcga
     4741 cggcgaggac acccacatgc tcgagcgcgt ccagaaggcg gcgtgcgatc tcctcggcct
     4801 cactctggag gcccaccgcc gccggagccc gagagacgcg gaggatgccg ctctgatgaa
     4861 cgttctcggc gagcggatag aagtcggtct cgccgccccg gccccgcacc gcgatgatcg
     4921 agagctcgcg gtcgaagtcc accagctcct cggccagctc gtcggggccg agcgtctctg
     4981 ccgcctcgac gagccgttgt cccttgccgt cgtagccgag tcgccgcttc ttcacgagcg
     5041 ccgggaggcc aacgtcggcc agctcgccga agcgcgctgt gggaatgccg aggcggcgga
     5101 aaagctgctt ttcgcgtaat cggtcctgac cttgctcgag cgagcgcggg cccggcacag
     5161 cgccgacgcg acgggccgcg tcgaccggaa cgttctcgaa ctcgaacgtc accgcagccg
     5221 ctcctgcgct cagccggtcg agcagagcgg ggtcgtcgta cgcaccgacg agtagctcac
     5281 ccaccgcgcc ggcacacgcg tccggagagg ggtcgaggaa gcggaaggag accccgaggt
     5341 tgattcccgc gagtgccagc atccgtccca gctggccgcc gccgatgcag ccgacgatcg
     5401 tcaaccagcc ggatcggggc ggtcgagcac cgcctgcgtc tgctcggcac ggaagcgctc
     5461 gagccgttcg cggatcccgg cgtcgccacg gccgaggatc gcagctgcca gaagagcggc
     5521 gttgacggcc cccgcgcgcc cgatcgcgac ggtgccgaca gggactccgg cgggcatctg
     5581 cacgatcgac aggagcgagt cgacgccctt cagcgccttc gactcgacgg gaacgccgat
     5641 caccggcagc acggacttcg acgccgccat gccggggagg tgagctgctc cgccggcgcc
     5701 ggcgatcagc acctggaggc cccgcgcctc ggcacccgcc gcgtactcga aaaggagatc
     5761 cggcgttcgg tgcgcggaga cgacgcgacg ctcgtatggg acgcccatct gctccaacgt
     5821 gtcggccgcg tgccgcatcg tctcccagtc ggagcgcgag cccatgatca ggccaacgag
     5881 cggctcagcc acggtgcgca ggctacttcg tccgcgactt cttcgtgagg aagagggcca
     5941 cgctccgagc cgtcttccgc acgtctgcgc cctcgtcgtc gttctcggcg attccgtcga
     6001 gcagctggat cgagtcgcgc tgcggcgacc cgtttcgcag gctgacgatc gcgaggcgcc
     6061 ggacggcgac gttcgaatcg tgggcggcga gctcgtgcag ctgcggccgg tactggttga
     6121 cgtcgccggg atgctcccgc gagagcgact cgagcgcgat cagggcggag ccgcgagcag
     6181 ccgggctctc gtcctcgaga ccgcgccgga gcaactcgag cttctgcctg aaacggaact
     6241 gagccagagc tcggtaggcc tgcccccgta tccgatagtc ggggttgcgg gccgcggcct
     6301 cgatttcgtc cgcccattcg gcgcggagcg cggcgagttc gcgctccaga ccgcgctgcg
     6361 cgtactcgtc cgcgagcgcc gcggcctcgt ggagctccac tacgggagcg ggcgcgagcc
     6421 cggcccgacg tagcgggcgg agggccggac gaggcgactg agcttcttct gctcgatgat
     6481 gtgcgccgac cagcctgccg tccgcgagca gccgaacatc gcgggcgcca gcttgggcgg
     6541 cacctcggcc acgtcgagca ccaccgccga ccagaactcc acgttcgtcg ccagaactcg
     6601 gtctggcttt cgcgcgtgca gctccgccag cgccacccgc tcgagctcct cggcgacgtc
     6661 gaaccgaggc gagcccagct ctttcgccgt gcgacggagc aagcgggcgc gcggatcctc
     6721 ggcccggtag acgcggtggc cgaagcccat cagccgctcg ccggaatcga gaagatcctt
     6781 gacgaaggaa tcgacgtcgt cggctgcggc ggccgcgtcg agcatcggca gaacacgggc
     6841 cggcgcacct ccgtgcagcg ggccggagag cgcaccgacc gcggaagaga ggcaggcggc
     6901 acagtctgcg cccgtggagg cgaccacacg tgcggtgaac gtcgaagcgt tcatcccgtg
     6961 ctcggcggtg
//
LOCUS       DBNGNK010000310         4190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1270462,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000310 DBNGNK010000000
VERSION     DBNGNK010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1270462"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="AC150V_09140"
     CDS             <1..381
                     /locus_tag="AC150V_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR3763122.1"
                     /translation="LLEGIGLLDKRDEYPSRLSGGQQQRVAIVRALAMEPRLLLLDEV
                     TSALDPELVAEVLEVVRELAAGGMTMLIATHEMGFARDVAHRVCFLDGGVILEQGTPE
                     QIFTAPREPRTEQFLQRIVEAGRL"
     gene            400..690
                     /locus_tag="AC150V_09145"
     CDS             400..690
                     /locus_tag="AC150V_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012393.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3763123.1"
                     /translation="MVQDPQRIDLSLEPLDDHLVVHPSDEESETRTGLIIPASTSTEC
                     QTGIVTAVGPEATGLEPGDKVLFPKDSGYDVRLSGTEVKVLRRSDLIARVHD"
     gene            755..846
                     /locus_tag="AC150V_09150"
     tRNA            755..846
                     /locus_tag="AC150V_09150"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:789..791,aa:Ser,seq:gct)
     gene            897..1487
                     /gene="recR"
                     /locus_tag="AC150V_09155"
     CDS             897..1487
                     /gene="recR"
                     /locus_tag="AC150V_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR3763124.1"
                     /translation="MLSPSVENLVAQLTRLPGIGARTAHRLAFHLLSVDKDEAHALAA
                     AIEEVKERVRFCRECGNLTEEELCAICTDQRRDRSVVCVVEHPVDVVSLERTHEYRGL
                     YHVLGGALSPLDGVEPGDIRIGELLHRVESGEVTEVIIATNPTMTGEATAGYLADKLR
                     GKARVTKLASGLPVGGDLEYADEVTLGRALAGRSEI"
     gene            1477..2208
                     /locus_tag="AC150V_09160"
     CDS             1477..2208
                     /locus_tag="AC150V_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR3763125.1"
                     /translation="MRSEEAIEPGFPVDASKTDSAVMSENDPAPSDALVVLKFGDSAV
                     ADSHRLKNVARCLVGEHERGRRVIGVLAAIGHTTDDLVALAHAVSPQPQPRELDMLVS
                     VGERVACALCAMAIIDLGHKAVSLTGSQAGIVTDTAHGDAHIVEVRGRRIEQALEADS
                     IVLVAGFQGVSTEHEVTTLGPCGSDITAIALASALGAASCEIVTDVAAVEELPLRSVE
                     FARDVGVTLRLRSPSQLGAGPLVAA"
     gene            2226..3188
                     /locus_tag="AC150V_09165"
     CDS             2226..3188
                     /locus_tag="AC150V_09165"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011934486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR3763126.1"
                     /translation="MSGSPRIGVIGATGAVGKITVELLRERGYENLRTFASARSAGST
                     LDGLVVEEATPDVLSAGDLDLCLFSVGTSASRELVPPASEAGAICVDKSSAYRLVDGY
                     PLVVPEVNGRRAIESLERDRIVANPNCCAIPLTCVLKPLHDLAGLRRVHVATYQSVSG
                     AGAQRMEALRNERSDDHDLLMDWSWEGDESDEESKLRAETRKILELPELSISATCVRV
                     PVLVGHSEAVWIEFAEDVSAAEAAAALRAAPSVQVLELPEFPTPRAATGIDDVLVGRI
                     RQDGPAGLALFLSSDNLRKGAALNAIQIAELLLHADRSVTSARG"
     gene            3219..3305
                     /locus_tag="AC150V_09170"
     tRNA            3219..3305
                     /locus_tag="AC150V_09170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3253..3255,aa:Ser,seq:cga)
     gene            3415..3822
                     /locus_tag="AC150V_09175"
     CDS             3415..3822
                     /locus_tag="AC150V_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR3763127.1"
                     /translation="MRRPFVVTAALAALLALIATAAAFGVGKQSQDVTVTLRDFKIVQ
                     KSRLPAGRTTIRFQNKGKFDHNYKVVYKSAGATKIVTKDIKPGKTATLKVNLKPGTYT
                     VLCTVFNGFHASQGMVKQFTVGKIDFDTGKWGT"
BASE COUNT          678 a         1402 c         1426 g          684 t
ORIGIN      
        1 ctgctcgagg gcatcgggct gctcgacaag cgcgacgagt acccgtcgcg gctctccggc
       61 ggacagcagc agcgcgtcgc gatcgtgcgc gcgctcgcga tggagccgcg gctgctgctg
      121 ctcgacgagg tgacgagcgc gctcgacccg gagctcgtcg ccgaggtgct cgaggtcgtg
      181 cgcgagctcg ctgcgggcgg tatgacgatg ctgatcgcca cgcacgagat gggcttcgcc
      241 cgcgacgtgg cgcatcgagt ctgcttcctc gacgggggcg tgatcctcga gcagggaacg
      301 cccgagcaga tcttcaccgc gccgcgcgag ccccgcaccg agcagttcct ccagcgcatc
      361 gtcgaggccg gtcggctgta gggttatctt tgtaggtccg tggttcagga tccgcaacgc
      421 atcgacctgt cactcgagcc gctcgacgat cacctcgtcg tgcatccctc cgacgaggaa
      481 tcggagaccc gcacggggct gatcattccc gcttcgactt cgacggagtg ccagacgggc
      541 atagtgacgg cggtcggccc ggaggcgacc ggcctcgagc cgggcgacaa ggtgttgttt
      601 ccgaaggact cgggctacga cgttcgcctg agcggtaccg aggtgaaggt tctccgccgc
      661 agcgacctga tcgcccgagt gcacgactag ccgacggtct gcgcaggggt cttccccccg
      721 gctccgaaac gtgccttgta tcctgtgacc ccagggagaa gtggccgagt ggctgaaggc
      781 ggcgccctgc taaggcgtta ggtggggaga atccctgcct cgagggttcg aatccctcct
      841 tctccgtctc cagttcccgc cgggggctgt ccgttccggc cgactacgct gctcccgtgc
      901 tcagcccctc ggtcgagaac ctcgtcgcgc agctgacccg gcttcccgga atcggagcgc
      961 gaaccgcaca ccggctcgcg ttccacctgc tctcggtgga caaggacgag gcgcacgcgc
     1021 tggccgctgc gatcgaggag gtcaaggagc gggttcgctt ctgccgcgag tgcggcaacc
     1081 tgacggagga ggagctgtgc gcgatctgca cggatcagcg ccgcgaccgc tccgttgtct
     1141 gcgtcgtcga gcatccggtc gacgtcgtct cgctcgagcg cacgcacgag taccgcggcc
     1201 tctaccacgt gctcggcgga gcgctgtctc ctctcgacgg tgtcgagccg ggcgacatcc
     1261 gcatcggcga gctgctgcat cgagtcgagt ccggcgaggt caccgaggtc atcatcgcca
     1321 cgaatccgac gatgaccggc gaggccaccg ccggctacct ggccgacaag ctccggggca
     1381 aagcccgagt gacgaagctc gcgagcgggc ttcccgtggg cggagacctc gagtacgcgg
     1441 acgaggtcac gctgggacgg gcgctcgcgg ggcgcagtga gatctgagga ggcgatcgag
     1501 ccgggattcc ccgtggatgc gtcgaagacc gactcagctg tcatgagcga gaacgacccc
     1561 gcaccgagcg acgcgctcgt cgtgctcaag ttcggcgaca gtgcggtcgc cgactcgcac
     1621 cgactgaaga acgtcgcgcg ctgcctcgtc ggcgagcacg agcgcgggcg cagggtgatc
     1681 ggcgtgctgg cggccatcgg ccacaccacg gacgacctcg tcgcgctggc tcacgccgtc
     1741 tctccgcagc ctcagccgcg cgagctcgac atgctcgtct cggtcggcga gcgtgtcgcg
     1801 tgcgctctct gcgcgatggc gatcatcgac ctcgggcaca aagcggtctc gctgaccgga
     1861 tcgcaggcgg gcatcgtgac cgacaccgcg cacggcgacg cccacatcgt cgaggtgcgc
     1921 ggtcgtcgca tcgagcaggc gctcgaagcc gactcgattg tcctcgtcgc cggcttccag
     1981 ggcgtgtcca ctgagcacga ggtgaccacg ctcggtccgt gcggctcgga tatcacggca
     2041 atcgcactcg cgtcggcgct cggcgcggcg tcatgcgaga tcgtcacgga cgtggcagcc
     2101 gtcgaggagt taccgctacg ctcggtggag ttcgcacgcg acgtcggcgt cacgttgcgg
     2161 ctgcggtcgc cgtcccagct cggcgccggg ccgctcgtgg cggcctagag aggagagcga
     2221 cgaaggtgag cgggagccca cgcatcggcg tcatcggcgc caccggcgcg gtcgggaaga
     2281 tcacggtgga gttgctgcgc gagcgcggct acgagaacct gaggacgttc gcctcggccc
     2341 gctccgccgg ctcgaccctg gacgggctcg tcgtcgagga agcgacgccc gacgtcctga
     2401 gcgcaggtga cctcgacctc tgtctctttt ccgtgggcac gtccgccagc cgcgagctgg
     2461 ttccgccggc gtccgaggcc ggcgcgatct gcgtcgacaa gtcgtccgcc tatcggctcg
     2521 tcgacggcta ccccctcgtg gttcccgagg tgaacggaag acgcgcgatc gagtccctcg
     2581 agcgcgaccg catcgtggcg aacccgaatt gctgcgcgat cccgctgacg tgcgtgctga
     2641 agccgctgca cgacctggcc ggcctccgtc gcgtccacgt cgccacgtac cagtcggtct
     2701 cgggagcagg agcgcaacgg atggaggcgc tgcggaacga gcgatcagac gaccacgact
     2761 tgctgatgga ctggtcctgg gagggagacg agtcggacga ggagtcgaag ctcagggccg
     2821 agacgcgaaa gatcctcgag ctgccggagc tctcgatcag tgcgacctgt gtccgtgtcc
     2881 cggttctggt cgggcacagc gaggccgtct ggatcgagtt cgccgaggac gtgtcggccg
     2941 ccgaggcggc ggccgcattg cgcgcggcgc cgtcggttca ggtcctcgag ctcccggagt
     3001 ttccgactcc gcgcgctgcg accgggatcg acgacgtgct cgtcggccgc atccgccagg
     3061 acgggcccgc gggtcttgcg ctcttcctct ccagcgacaa cctccgcaag ggagcggcgc
     3121 tcaacgcgat ccagatcgct gagctcttgc tccacgcgga tcgctcggtc acctccgccc
     3181 gcggctgaga ggcgcaccgg atacccttcg ccctcgccgg aggggtgccg gagcggtcga
     3241 acggggcggt ctcgaaaacc gttagacgtg caagcgtctc gagggttcaa atccctcccc
     3301 ctccgcctca gccccgtgcg gatcttcggc ggttttgtcc tggcatcgga aaccgttcga
     3361 tgcgggggtg gcgtatggac cggtaacggg attgcctatc gaaaaggagg gaacatgcgc
     3421 cgaccgttcg ttgtaacggc agcgctcgcg gccttgctgg cgctcatcgc gacagcagca
     3481 gccttcggtg ttggtaagca aagccaggac gttaccgtga ccctgcgtga cttcaagatc
     3541 gttcaaaagt cgaggctgcc ggccgggcga acgacgatca gattccagaa caagggcaag
     3601 ttcgaccaca actacaaggt cgtgtacaag tcggccggcg ccaccaagat cgtcaccaaa
     3661 gacatcaagc cgggcaagac cgccacgctc aaagtgaatc tcaagcccgg cacctacacg
     3721 gtgctctgca ctgtcttcaa cggtttccac gcgagccagg ggatggtgaa gcagttcacc
     3781 gtagggaaga tcgacttcga taccggcaag tggggcacat agcgtcttgc tcacctgtca
     3841 ggtgttgacg acctgaggat ttggtcgctg gacggtctcg gagggacgca gccccgaaga
     3901 gatcgtccgg cggtcgactc aagaaggccg ctcgaccgga tccgatcgga cctcccgttg
     3961 ctcgacccga agtcgttgga cgtgcaagcg tctcgagggt tcaaatccct ccccctccgc
     4021 ctcaaaccaa gccccggcga gtagcggcct ggagccgaat ccgcccagac cacggcgcag
     4081 tggccgaatc ggtgacgccg ttgctatgcc ctggtcgaga cgatgaggtc gtcttccgca
     4141 ggctcgccgc gccggagctc gcggctccgc tcggcctcgg cgttgatctc
//
LOCUS       DBNGNK010000311        11911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1486026,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000311 DBNGNK010000000
VERSION     DBNGNK010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 11911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11911
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1486026"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="AC150V_09180"
     CDS             complement(<1..747)
                     /locus_tag="AC150V_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010510869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR3763128.1"
                     /translation="MCGIAGIIYRDGAEHDVGVDMTRMLQSMKHRGPDSSGYALYRKP
                     EDDVIVMRYKLADANDPRDYEFAERLERHRREVEARLETLGAKLEEVDFDTEYAFRVR
                     MRYGGDLKAIADFVEDVPGAEVLSLGRALEIVKDLGDAETVSEQYHLEKIRGTHAIGH
                     VRMATESDVDISGAHPYWAYPFSDTAVVHNGQLTNYFHWRRRLERTGHRFQSECDSEI
                     IAVYLAEKMANGASLKEAMDQSLDELDGVFT"
     gene            complement(795..2192)
                     /locus_tag="AC150V_09185"
     CDS             complement(795..2192)
                     /locus_tag="AC150V_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR3763129.1"
                     /translation="MATDLEQFVQAEGRDELVKEVRRRIDAEGIKYIYYQFASVTGRI
                     MGKGVPAVYWERMAEKGFQLVYGATANLFTDRYDHYIGYGPEAAELVGIPEPETFQPL
                     PWDEKVARVWCTCFRNREEREDPGAYLTSDCRGNLRRAQAAFEEKTGLHLRAGTEPEM
                     MWLKLDEDGTPSVEGVTKPYCYHIDQFSELQPIIHKVVEYGQVMGLEMIQGDHEDAPG
                     QLELNFDFDRAEQTADNLTTFRQICKAVGRELGVFPCFMPKPFMGVSANGCHHTISLW
                     RGDENVFMPDTDDPQRPGQHGLWAIGGIIEHLGALTAVCSPTVNSYRRLWDTGFWAPV
                     FADWGYQNRTTALRVSGPGRFEYRSVDSAVNPYLSLASLIAAMADGIERKLDPGDPEE
                     RNIYEAMEAGKEVRRLPMTFGDALDALEADEVIRGALPGDMYRVFMHYKRDEWERHCA
                     HVSDWDVEEYLDVLP"
     gene            2305..2757
                     /locus_tag="AC150V_09190"
     CDS             2305..2757
                     /locus_tag="AC150V_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR3763130.1"
                     /translation="MNEPKAEHLTQSQREALEAELAELEGPRRAEAVQAIKTAREYGD
                     LSENFEYHAAKNEQGLLEARIRRLRHRLAHAVTVEHETDDHVGVGSIVEIADESGETM
                     EVEISAVGGVSPDSPLGSALLGAGVGDQVDVDAPRGAWQARVVAIRRS"
     gene            2881..3588
                     /gene="thyX"
                     /locus_tag="AC150V_09195"
     CDS             2881..3588
                     /gene="thyX"
                     /locus_tag="AC150V_09195"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR3763131.1"
                     /translation="MGVAEPIIADKTISVLDHGFVRLDSSMADDLSVVNAARVSFARR
                     RDEMDESDVGLVGFLLRERHGTPFEHNAFRFHIRCPIFVAREWFRHRIGSFNEFSMRY
                     AKATEDFYVPAAEDVRSQVGKPGSYSFETVDETVAEQTREEMEAVYARAYAAYERLTE
                     AGVARELARTVLPVGAYTEFYWTVNARALMNFVSLRNSETAQREIRYYAEACEVFLAE
                     HMPVTYAAFVANDRVAP"
     gene            complement(3616..4194)
                     /locus_tag="AC150V_09200"
     CDS             complement(3616..4194)
                     /locus_tag="AC150V_09200"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HIR3763132.1"
                     /translation="MSGPTFDELHGPAESPEERRRLERTHDLLVAAGAPPELPPGLQP
                     VPAETRLFPRRRRFALVAVAAALGAAMFGVGYLVGDQGDGRDPSRVFALAGTQAAPSA
                     RGSLTMFAADVAGNLAIELTIRGLPKLPAGETYELWLTKDGKPVARSGSFVVEDEETA
                     VVELNTPWQPTDEHGWAVMRHGTTEALVRSSA"
     gene            complement(4191..4769)
                     /locus_tag="AC150V_09205"
     CDS             complement(4191..4769)
                     /locus_tag="AC150V_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3763133.1"
                     /translation="MPPHAEAATIEVVYEALTDGELIERIAEHDRDAFDEVYRRYARA
                     VLGLAVRRLGDRGRAEDVTQETFAAVWRSAARYDPGKGPGAPWLYTIARNAIVDGHRR
                     APEPPVEARDVVSDTDGPPEQAEAAWSAFRVHRALETLPEHERKVIELAYWSGLSQSE
                     ISDFLNVPLGTVKTRVRTGLARMADILEEDVR"
     gene            complement(4870..5481)
                     /locus_tag="AC150V_09210"
     CDS             complement(4870..5481)
                     /locus_tag="AC150V_09210"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3763134.1"
                     /translation="MAYTVPDLPYPYEALEPQIDAETMQIHHDKHHAAYVEKLNAALD
                     GTEWMGRPIDAVLSNLEILPAEIQTAVRNNGGGHANHTLFWEIMSPTGGGEPGGDLAA
                     TIIDTFGAFDVMQRHVIDAGINRFGSGWAWLVWDGTGLAVVSTANQDSPVMDGSVPLL
                     GIDVWEHAYYLTYRNRRPDYLEAWWNVVDWNVVGERFAAARSS"
     gene            5559..6170
                     /locus_tag="AC150V_09215"
     CDS             5559..6170
                     /locus_tag="AC150V_09215"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR3763135.1"
                     /translation="MSTVEEITARPLKPAVLELDGISRTTIEAHHRLYQGYVGKRNEI
                     LGKLAEVDLDAANQVLSDLRTLKVELTFAIGGIKNHELYFEHLGGPGGDPDGPIRELL
                     ERDFGSVDAWRADLKATGMAGRGWAWTAYDRDERRLFNYIGDAQNTFPVWNATPLVAL
                     DVYEHAYFLDYQTDRAAYIDAFFANLDWRVINGWLDAYGVRRS"
     gene            6204..6812
                     /gene="plsY"
                     /locus_tag="AC150V_09220"
     CDS             6204..6812
                     /gene="plsY"
                     /locus_tag="AC150V_09220"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR3763136.1"
                     /translation="MITDVLLVAVGYVAGSMPFGYWLPLLARGEDIRRHGSGNVGASN
                     VFRAYGRSLGIPVALLDVLKGFAPTLLALQLGGEWVGVMAGAAAMLGHARPLFLRFQR
                     GGKMVATAGGVAFALAPLAAACCLGVWVAVFVLTRYASVASLATALALPLFCLLFGEP
                     WPTVVFAAAAFVAVAFVHRQNIRRLLAGEESRFELRRRRAQA"
     gene            complement(6818..7804)
                     /gene="mptA"
                     /locus_tag="AC150V_09225"
     CDS             complement(6818..7804)
                     /gene="mptA"
                     /locus_tag="AC150V_09225"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HIR3763137.1"
                     /translation="MSKHLHVEEDVQASTPDIQLSLSRAGVTGVQKAIRIRRGDAETL
                     MAAEIDCTVDLDRSQKGVHMSRFPELFDEAIDTVVLGEALLIEVLAEHIAAHIVERQG
                     ALRAETRIVARWPMRRETPVTGLPTQEMVSLIGIAAARPGHARRVVGVEATGINACPC
                     AQGLVRGRAAERLLDAGFEDGDIERVLDLVPIATHNQRGRGTLFVGTAEDLDAEQLVE
                     IVEQSMSAPVYELLKRPDELFVVEHAHLRPRFVEDSVRGALSDAIERFPGLDDGDFLL
                     SRQVNLETIHDHDVVAERWGTAGELRRELAGNVEPGGRAESQTTLEAWLDAV"
     gene            complement(7837..8238)
                     /locus_tag="AC150V_09230"
     CDS             complement(7837..8238)
                     /locus_tag="AC150V_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3763138.1"
                     /translation="MPVGIGALASRTRTVTTRDIELFTELTGDRNPLHYDAAAAGRSR
                     FGEIIVQGGVTSGLLNAVVAEDLPGPGSVFLHVDWKFTAPVRPGDEITAAVEVLEARR
                     DKPITKLRTTVTRQDGTVVLEGTAVVWTEPL"
     gene            complement(8249..9076)
                     /locus_tag="AC150V_09235"
     CDS             complement(8249..9076)
                     /locus_tag="AC150V_09235"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR3763139.1"
                     /translation="MPELPEVEAWVRELDPLVSQSPIERAGPAHIATLKTAEPPLAAL
                     DGRRLAGARRRGKNILFPTSDDDLVLRVHLMSAGRLRWLKAGAKGPKTPMFRLRFTTG
                     TELVLTEAGKKKRAGVWLLDPDRLEQELAHLGPDALELDDESLAEILTRERRRLHPLL
                     RDQRALAGIGRAHANEILWRAELSPFALSTDLDATGVRRLRTALVEDLTRALELREQG
                     KGDKDVYLVHKRLGDPCPRCEAPLARVDYEEHTIYYCPSCQTGGRVLKDRRLSRLLR"
     gene            9324..10421
                     /locus_tag="AC150V_09240"
     CDS             9324..10421
                     /locus_tag="AC150V_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3763140.1"
                     /translation="MLTVEIALPDVEELHKLVERGQEKGFLSYDEIVSSLEDVDLTKE
                     QVEDFYTYLIDHSIELVEGEQHKSPPHEQPALAEDEKPAPKLDLTVEPSLDSLRLYLR
                     EIGKVPLLTADQEVTLAKRIERGDMAAKTHMIEANLRLVVSIAKGYLGRGLSFLDLIQ
                     EGSLGLIRAVEKFDYRKGYKFSTYATWWIRQAVTRAIADKARTIRIPVHMVEKLNKVV
                     HIERQLVQRLGREPRPEEIAEELDMEPEEVREILRMSQLPVSLEKPIGEEEESSLGDF
                     VPDEQAESPFDTASLSLRREDVELALASLPERERKVIELRFGLCGTQPCTLEEVGRTF
                     GVTRERIRQIENNTLKRLESLPEAQALKDCV"
     gene            10531..11214
                     /locus_tag="AC150V_09245"
     CDS             10531..11214
                     /locus_tag="AC150V_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HIR3763141.1"
                     /translation="MNYDITRRVIAEFIGTFVLVIVVMGSLTVAGQSGLIGTAVAQGF
                     VLAILVSSLGHISGGHFNPAVTLGFLVTRRIEPIRGVAYAVAQFAGAIGAALLLNAMY
                     PAEANLDSTVTTVNETFSAFEGMILEGIFTFLLVWVYFATAVDKKGTFASIGGFGIGL
                     VLTVSVLVIGPITGSSINPARTFGPALVENVWSDAWVYYVGPLVGGALAAVLYTALYL
                     PRRAEGQAG"
     gene            complement(11279..>11911)
                     /locus_tag="AC150V_09250"
     CDS             complement(11279..>11911)
                     /locus_tag="AC150V_09250"
                     /inference="COORDINATES: protein motif:HMM:NF025210.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR3763142.1"
                     /translation="LEMSASGAKVLMLRSVELGRNHGVRIHARSTFSDEPGTWIEEES
                     RMEQPIVSAVTHSETDVVFTLGDIPDRPGVAALIFDVIAQTNVNVDTIMQNVVHGKAE
                     ISFSVPAEDVPTTRAAIETAQSELGAMSVEENHGLGKVSLVGAGMRSHPGVAAKMFRT
                     LADQGINLEMISTSPIKVSCMISRGEIPGAVRSLHAAFELDREPQAEPTI"
BASE COUNT         1823 a         4014 c         4038 g         2036 t
ORIGIN      
        1 cgtgaacacg ccgtcgagct cgtcgaggct ctggtccatc gcctccttca gcgacgcacc
       61 gttcgccatc ttctcggcca gatagaccgc aatgatctcg gagtcgcact cggactggaa
      121 ccggtgacct gtccgctcaa gccgtcgccg ccagtggaag tagttggtca gctggccgtt
      181 gtgcacgacg gcagtgtcgg agaaggggta ggcccagtac gggtgggcgc ccgagatgtc
      241 cacgtccgac tcggttgcca tgcggacgtg accgatcgcg tgcgtgcccc ggatcttctc
      301 gaggtggtac tgctcggaga ccgtctccgc gtcgccgaga tccttgacga tctcgagcgc
      361 gcggccgagc gagagcacct cggcgccggg aacgtcctcg acgaagtcgg cgatcgcctt
      421 gagatcgccg ccgtaccgca tccgcacgcg gaaggcgtac tcggtgtcga agtcgacctc
      481 ctcgagcttc gcgccgagcg tctcgaggcg ggcctcgacc tcgcgccggt gccgctccag
      541 tcgctccgcg aactcgtagt cgcgcgggtc gttggcgtcc gcaagcttgt accgcatgac
      601 gatgacgtcg tcctccggct tccgatacag ggcgtacccg ctcgagtcgg ggccgcggtg
      661 cttcatcgat tggagcatgc gggtcatatc caccccgacg tcgtgctccg ccccgtcccg
      721 gtagatgatt cctgcgatcc cgcacatagg cgtgtccttt cgccccctat tctctcctag
      781 ggcagcacgt ctctctaggg cagcacgtcg aggtactcct cgacgtccca gtcgctcacg
      841 tgggcgcagt ggcgctccca ttcgtcgcgc ttgtaatgca tgaagacacg gtacatgtcc
      901 ccgggcagag cgcccctgat cacttcgtcg gcctccagcg cgtcgagcgc gtcaccgaag
      961 gtcatcggca gcctcctgac ctccttgccc gcctccatcg cctcgtagat gttccgctcc
     1021 tcggggtcgc ctgggtcgag cttgcgctcg atcccgtcgg ccatggccgc gatgaggctc
     1081 gccagcgaca ggtacggatt gaccgccgag tcgaccgagc ggtattcgaa gcggccgggt
     1141 ccggacacgc gcagggcggt cgtccgattc tggtagcccc agtcggcgaa gacgggggcc
     1201 cagaagcctg tgtcccagag gcggcggtag gagttcacgg tcggcgagca gacggcggtg
     1261 agcgcgccga ggtgctcgat gatccccccg atcgcccaga ggccgtgctg gcccggcctc
     1321 tgcggatcgt cggtgtcggg catgaagacg ttctcgtcgc cacgccagag cgagatggtg
     1381 tggtggcagc cgttcgcgga cacgcccatg aagggcttcg gcatgaagca ggggaagacg
     1441 ccgagctcgc ggccgactgc cttgcagatc tgccggaagg tggtgaggtt gtccgccgtc
     1501 tgctcggcgc ggtcgaagtc gaagttgagc tcgagctggc cgggcgcgtc ctcgtgatcg
     1561 ccctggatca tctcgaggcc catcacctgc ccgtactcga cgaccttgtg gatgatcggc
     1621 tgcagctcgg agaactggtc gatgtggtag cagtacggct ttgtgacgcc ctcgaccgag
     1681 ggcgtgccgt cctcgtcgag cttgagccac atcatctctg gctcggtgcc ggcacgaagg
     1741 tgcaggcccg ttttctcctc gaaggccgcc tgcgcgcgcc tgaggttgcc ccggcagtcg
     1801 gacgtcaggt aggcgcctgg atcctcgcgc tcctcgcggt tgcggaagca ggtgcaccag
     1861 acgcgtgcga ccttctcgtc ccagggcaac ggctggaagg tctccggctc ggggatgccg
     1921 acgagctctg ccgcctccgg cccgtagccg atgtagtggt cgtaacggtc ggtgaagagg
     1981 ttcgcggtgg cgccgtagac gagctggaat cccttctcgg ccatccgttc ccaatacacc
     2041 gccggaactc ccttgcccat gatgcggccg gtgaccgacg cgaactggta gtagatgtac
     2101 ttgatccctt cggcgtcgat ccgccgtcgg acctccttca caagctcgtc gcgaccctcc
     2161 gcctggacga actgctcaag atccgtcgcc actccgatcc tccttccttg ctccggaatc
     2221 aatgcgaccg gagcgtcgtg atcgacccgt ggcgcgggag gatactacgg ctccggcggg
     2281 gcgaccgttg gctagccttg ccggatgaac gagccgaagg cagagcacct gacgcagtcg
     2341 cagcgggagg ctctggaggc cgagctcgcg gagctcgagg ggccgcgtcg cgcggaggcc
     2401 gtccaggcga tcaagaccgc ccgtgagtac ggcgacctgt cggagaactt cgagtaccac
     2461 gccgcgaaga acgagcaggg cctgctcgag gcaaggatcc gcaggctacg tcaccggctg
     2521 gcgcatgcgg tcacggtgga gcacgagacc gacgatcatg tcggcgtcgg ctctatcgtc
     2581 gagatcgccg acgagagcgg cgagacgatg gaggtcgaga tctcggccgt cggcggcgta
     2641 tcgccggact caccgctcgg cagtgcactg ctcggggccg gtgtgggcga ccaggtcgac
     2701 gtggacgcgc cgcggggcgc gtggcaggcg cgcgtcgtcg ccattcggcg ctcctagccc
     2761 gctcgctgct cgcttctcgg gcagggtatg tggcagttcg gtcacacagg tcctcagagg
     2821 cgggacgtgg ctgtttcggc cctagcggct ccggccggag gcggcggtag actcgctcgc
     2881 gtgggagttg cggagccgat catcgccgac aaaacgatca gcgtcctcga ccacggcttc
     2941 gtcaggctcg acagctcgat ggccgacgac ctgtcagtcg tcaacgcggc gagggtcagc
     3001 ttcgcccggc gccgcgacga gatggacgag tcggacgtcg ggctggtcgg cttcctcctg
     3061 cgagagcgcc acggcactcc cttcgagcac aacgcattcc gctttcacat ccgctgcccg
     3121 atcttcgttg cgcgcgaatg gttccggcac cggatcggct cgttcaacga gttcagcatg
     3181 cgatacgcga aagcgacgga ggacttctac gtccccgccg ccgaggacgt gcgatcccaa
     3241 gtcggcaagc cgggctcgta ctccttcgag acggtcgacg aaacggtcgc ggagcagacg
     3301 cgcgaggaga tggaggccgt ctacgcccgt gcctacgccg cctacgagcg gctcaccgag
     3361 gccggcgtcg cacgcgagct ggcccgcacg gttctgcccg tcggcgccta cacggagttc
     3421 tattggacgg tcaacgctcg tgcgctgatg aacttcgtct ccttgcgcaa ctcggagacg
     3481 gcgcagcgcg agattcgcta ctacgccgag gcgtgcgagg tcttcctcgc tgagcacatg
     3541 cctgtcacgt acgcggcgtt cgtcgccaac gaccgcgtgg ccccgtagcg cacttcacgt
     3601 tgtcggcacg tccgtctacg cggaagacct gacgagcgcc tcggtcgtgc cgtggcgcat
     3661 gaccgcccac ccgtgctcgt ccgtcggctg ccacggcgtg ttgagctcca cgaccgccgt
     3721 ctcttcgtcc tcgaccacga aggagccgct ccgggcgacc ggcttgccgt ccttcgtcaa
     3781 ccagagctcg tacgtctcgc ccgccggaag cttcggaagc cctcggatcg tgagctcgat
     3841 cgccaggttg ccggcgacgt cggccgcgaa catcgtgagc gagccacggg cgctcggcgc
     3901 ggcctgcgta cctgcaagcg cgaagacgcg gctcgggtct ctcccgtcac cctgatcccc
     3961 gacgagatag ccgacaccga acatcgcggc accgagcgcc gcggcgaccg cgacgagagc
     4021 gaagcgcctg cggcggggaa agaggcgagt ctccgccgga accggctgca gcccgggcgg
     4081 cagctcgggc ggtgcgcccg ccgccacgag gaggtcgtgc gtccgctcga gccgccggcg
     4141 ttcctccggc gactcggcag ggccgtggag ctcgtcgaac gtcggcccgc tcaccggacg
     4201 tcttcctcga ggatgtctgc catccgggcc agaccggttc gcacgcgcgt cttgaccgtg
     4261 ccgagcggca cgttgaggaa gtcggagatc tcgctctgcg agagtccgct ccagtaggcg
     4321 agctcgatga ccttccgctc gtgctccggc agagtctcga gcgctcggtg aactcgaaag
     4381 gcggaccagg ccgcctcggc ctgctccggc ggcccgtctg tgtcggagac gacgtcccgc
     4441 gcctccaccg gcggctcggg tgcccgcctg tggccgtcga cgatcgcgtt gcgggcgatc
     4501 gtgtagagcc atggagcccc ggggcccttc ccggggtcgt atcgcgcagc tgatcgccac
     4561 acggccgcga atgtctcttg cgtgacgtct tcggcccggc cgcggtcgcc gagtcgacgg
     4621 acggcgagcc cgagcacggc gcgcgcgtat cggcggtaga cctcgtcgaa cgcgtcgcgg
     4681 tcgtgctccg cgatgcgctc gatgagctcg ccgtcggtca gcgcctcgta cacgacctcg
     4741 atcgtggccg cttcggcatg cggcggcaag cgcatggcgt atgcacaacc catcggtgat
     4801 gcctacgctc ggcgtcgccg gtcggattgc cggtggcgcg caggtgggcc atgggatcga
     4861 gcgcggagcc taggacgacc gtgctgcggc gaagcgctcg ccgacgacgt tccagtcgac
     4921 gacgttccac catgcctcga gatagtcggg gcggcggttt cgatacgtca ggtagtacgc
     4981 gtgctcccag acgtcgattc cgagcagagg gacgctgccg tccatcacgg gactatcctg
     5041 gttcgctgtc gagacgacgg cgagcccggt gccgtcccag accagccacg cccaaccgct
     5101 cccgaagcgg ttgatccccg cgtcgatgac gtgccgctgc atcacgtcga acgcgccgaa
     5161 ggtgtcgatt atcgtggccg cgaggtcgcc gccgggctcg cctccgcccg tcgggctcat
     5221 gatctcccag aacagcgtgt ggttggcgtg gccgccgccg ttgttcctga ccgccgtctg
     5281 aatctcagcc ggcaggatct ccaggttgct gagcaccgcg tcgatcggcc ggcccatcca
     5341 ctcggtgccg tcgagggcgg cgttgagctt ctcgacgtag gcggcgtgat gcttgtcgtg
     5401 gtggatttgc atcgtctcgg cgtcgatctg gggctccagc gcctcgtacg ggtagggaag
     5461 gtcgggaacc gtatacgcca tcgtcagctc cttctcgagt tcatcggggt cgtctcaagg
     5521 ttatgctccg gcccgtccaa gtccgagcag gaggtgccat gtcgacagtc gaggagatca
     5581 ccgcccgtcc gctgaagccc gcagtgctgg agctcgacgg catctcgcga acgaccatcg
     5641 aggcccacca ccgcctctac cagggctacg tcgggaagcg gaacgagatc ctcgggaagc
     5701 tggccgaggt cgatctcgac gcggcgaacc aggtgctctc cgacctgcgc acactgaaag
     5761 tggaactcac gttcgcgatc ggtgggatca agaaccacga gctctacttc gagcatctcg
     5821 gcgggcccgg gggcgacccc gacggtccca tccgtgagct gctcgagcgc gatttcggat
     5881 cggtcgacgc atggcgcgcc gacctgaagg ccaccgggat ggccgggcgc ggctgggctt
     5941 ggactgcgta cgaccgggac gaacggcgcc tcttcaacta catcggcgac gcgcagaaca
     6001 cgttcccggt ctggaacgcg acccccctgg ttgcgctcga cgtctacgag catgcgtact
     6061 tcctcgacta tcagaccgac cgagccgcgt acatcgacgc cttcttcgcg aacctcgact
     6121 ggcgtgtgat caacggctgg ctcgacgcgt acggcgtccg caggtcgtag gctgacgcag
     6181 cccgccgccg cgcgggcccg gtcatgatca ccgacgttct cctcgtcgcc gtcggctacg
     6241 tcgccggctc catgcccttc gggtactggc tgccgctcct ggctcgcggc gaggacattc
     6301 gccggcacgg cagcggcaac gtcggcgcgt ccaacgtctt tcgcgcgtac gggcgatcgc
     6361 tcgggatccc agtcgccctg ctcgatgtgc tcaagggatt cgcgcccacg cttctggcgc
     6421 tacagctcgg cggcgagtgg gtcggtgtga tggccggcgc ggcggcgatg ctcgggcacg
     6481 cgcgcccgct cttccttcgc ttccagcgcg gcgggaagat ggtcgccacc gcgggcggcg
     6541 tggccttcgc cctcgcgccg ctcgccgccg cgtgctgtct cggcgtgtgg gtcgccgtct
     6601 tcgtcctgac gcggtacgca tcggtcgcct ctctggccac ggcgctcgcg cttcccctct
     6661 tctgcctcct cttcggcgag ccctggccca cggtcgtctt cgcagcggcc gcgttcgtcg
     6721 ccgttgcctt cgtgcatcga cagaacatcc gtcgtctgct cgccggcgag gagagtcgct
     6781 tcgagctccg tcggcggcgg gcgcaggcct gaacgcgcta gacggcgtcg agccaggctt
     6841 cgagagtcgt ctggctctcg gcccgtccgc cgggctcgac atttccggcg agctcgcggc
     6901 gaagctcgcc ggcggttccc cagcgctcgg ccacgacgtc gtgatcgtgg atcgtctcga
     6961 gattcacctg gcgagagagc aggaagtcgc cgtcgtcgag cccggggaag cgctcgatcg
     7021 cgtcgctcag cgcgccgcgg acggagtcct cgacgaagcg cgggcgcagg tgtgcatgct
     7081 cgacgacgaa gagctcgtcc ggtcgcttca acagctcgta gacgggagcg ctcatggact
     7141 gctcgacgat ctcgaccagc tgttctgcgt cgagatcctc ggccgtgccg acgaacagag
     7201 tgccgcggcc acgctgattg tgagtggcga tcgggacgag gtcgaggacg cgctcgatgt
     7261 cgccgtcttc gaagcctgcg tccagcagac gctcggcggc ccggccgcgc acgagcccct
     7321 gagcgcaggg gcaggcgttg atgccggttg cctccacgcc gacgacccgc cgcgcgtgac
     7381 cgggccgcgc cgcggcgatc ccgatcagcg agaccatctc ctgggtcggc aggcccgtca
     7441 ccggagtctc gcggcgcatc ggccaacgcg cgacgatccg cgtctcggcc cgaagtgcgc
     7501 cctgccgctc gacgatgtgg gcggcgatgt gctccgccag cacctcgatc agcagagcct
     7561 cgccgagcac aacagtgtcg atcgcctcgt cgaagagctc cgggaagcgc gacatgtgca
     7621 cgcctttctg actccggtcg aggtcgacgg tgcagtcgat ctcggccgcc atcagcgtct
     7681 cggcgtcgcc gcggcgaatg cggatcgcct tctgcacccc ggtgacgccc gcgcgggaga
     7741 gcgagagctg gatatcgggc gtcgaggctt ggacatcctc ctcgacgtgc aggtgcttgc
     7801 tcatcgggac acggacggta ccagcgcgcc gcggtgtcac agcggctcgg tccagacgac
     7861 ggccgttccc tcgagcacga ccgtcccgtc ctgtctggtg actgtcgtac gtagcttcgt
     7921 gatcggcttg tcgcgccgcg cctccaacac ctcgaccgcg gcggtgatct cgtcgcccgg
     7981 ccgaaccggg gcagtgaact tccagtcgac gtggagaaag acgctgccgg gaccgggcag
     8041 gtcctcggca acaacggcat tcaggagacc cgacgtcacg ccgccctgga cgatgatctc
     8101 gccgaagcgt gagcgcccgg ccgccgcggc gtcgtagtgg agtggattgc ggtcacccgt
     8161 gagctcggtg aagagctcga tgtcacgggt ggtgacggta cgggtgcggc ttgcgagggc
     8221 gccgatcccg actggcacgg ccacctctct acctcaacag ccgggacagc cgacggtctt
     8281 tcagcacccg cccgcccgtc tggcagctcg ggcagtagta gatcgtgtgc tcttcgtagt
     8341 cgacgcgggc gagcggcgcc tcgcaccgcg ggcacggatc gccgaggcgc ttgtggacga
     8401 gatagacgtc cttgtcaccc ttcccctgct cgcgaagctc cagcgcgcgc gtcaggtcct
     8461 cgaccaacgc tgtcctgagc cgcctgacgc cggtggcgtc gaggtccgtc gagagcgcga
     8521 acggcgagag ctccgcccgc cagaggatct cgttcgcgtg tgctcgcccg atgccggcga
     8581 gcgctcgctg gtcgcgaagc agtggatgca ggcggcgccg ctcccgcgtg agaatctccg
     8641 ccagcgactc gtcgtcgagc tccagcgcgt cggggccgag gtgcgcaagc tcttgttcca
     8701 ggcgatcggg gtcgagcagc cacacgcccg cacgcttctt ctttcccgcc tcggtgagaa
     8761 ccagctctgt gcccgtcgtg aagcgcagcc ggaacatcgg cgtcttcggc cccttggcgc
     8821 ctgccttcag ccagcgcagc cgcccggcgc tcatcagatg cacccgcaag acgaggtcgt
     8881 cgtcggacgt ggggaagagg atgttcttgc cgcggcgacg tgctccggcg agtcgcctgc
     8941 cgtcgagcgc tgcgagcggg ggctcggcgg tcttgagcgt tgcgatgtga gcgggcccgg
     9001 cgcgctcgat cgggctctgg gagacgagcg gatcgagctc tcggacccag gcctccacct
     9061 cgggcagctc gggcatcggt gggagcgtac cgcggggccg ctgtggaaaa acgggaactc
     9121 cggtcagccc caggctcgcg ccagaccggt aaacaccggc gaaaacgctc gggcgaccca
     9181 tgttaaggac gtgttcacaa atgtcagcga gatgtatagg attcgccgcc tgatggttcg
     9241 agccgggtga aacgcgtccg gctcgttgca cgttcggcag gtcagttccg tcaacccccg
     9301 agcggaagga gcaatccgag gcaatgctca ccgtcgagat cgccctccca gacgtcgagg
     9361 aactccacaa acttgtcgag cggggacaag agaaggggtt cctctcgtat gacgagatcg
     9421 tgtcctccct cgaggacgtc gatctcacca aggagcaggt cgaggacttc tacacgtacc
     9481 tgatcgacca ctcgatcgag ctcgtcgaag gcgagcagca caagagcccg ccccatgagc
     9541 agccggcact cgccgaggac gagaagccgg cgccgaagct cgacctgacg gtcgagccgt
     9601 cactcgactc gctccgcctc tatctccgtg agatcgggaa ggtgccgctg ctgacggcgg
     9661 accaggaggt cacgctggcg aaacgcatcg agcgcggtga catggcggcc aagacgcaca
     9721 tgatcgaggc gaacctgcga ctggtcgtct cgatcgccaa gggctatctc ggtcgcggtc
     9781 tcagcttcct cgacctgatc caggagggct ctctcgggct catccgggcc gtcgagaagt
     9841 tcgactaccg caagggctac aagttctcga cctacgcgac ctggtggatc cgtcaggcgg
     9901 tcacccgggc gatcgccgac aaggcgcgga cgatccgcat cccggtgcat atggtcgaga
     9961 agctcaacaa ggtcgtgcac atcgagaggc agctcgttca gcggctgggc cgtgagccgc
    10021 gaccggagga gatcgccgag gagctcgaca tggagcccga agaggtgcgc gagatcttgc
    10081 ggatgtcgca gctgcccgtg tcgctcgaga agccgatcgg cgaagaggaa gaatcgtcgc
    10141 taggggactt cgtcccggac gagcaggcgg agtcgccgtt cgacactgca tccctctcgt
    10201 tgcgacgcga ggacgtcgag ctggcgctgg cgtcgctgcc cgagcgcgag cgcaaggtga
    10261 tcgagcttcg cttcggactg tgcggcacgc agccgtgcac gctcgaggaa gtcgggcgta
    10321 ccttcggcgt gacgcgggag cggatccgcc agatcgagaa caacaccttg aagcggctgg
    10381 agtcgcttcc cgaggcgcag gcgctgaagg actgcgtcta ggcaagggct ggccccaacg
    10441 ggctgacgtc gggtaagggg ggccaagggg ctcccctcgc tcgttctacg ggctggaagg
    10501 ggtcacagca gggtcgccga ataggcggcc atgaactacg acatcacacg cagggtcatt
    10561 gcggagttca tcgggacgtt cgtgctcgtg atcgtcgtga tgggctccct cacggtcgcc
    10621 ggccagagcg gcctgatcgg gacggcagtc gcgcagggtt tcgtccttgc aatcctcgtc
    10681 tcgtcactcg gccacatctc gggcggtcac ttcaacccgg ccgtgacgct gggctttctg
    10741 gtcacgaggc gtatcgagcc gattcgcggt gtcgcctacg cggtcgccca gttcgccggt
    10801 gcgatcggtg cggctctgct cctcaacgcc atgtacccgg ctgaggcgaa cctcgactcg
    10861 acagtgacga ctgtgaacga gaccttcagt gctttcgagg gaatgatcct cgaagggatc
    10921 ttcacgttcc tgctcgtctg ggtctacttc gccaccgcag tcgacaagaa gggcacgttt
    10981 gcgtcgatcg gcggcttcgg catcggcctc gtgctgaccg tgtccgtgct cgtgatcgga
    11041 ccgatcaccg gctcctcgat caatccggcg cgcaccttcg ggcccgcact cgtcgagaac
    11101 gtctggagcg acgcctgggt ctactacgtc gggccgctcg tcggtggcgc tctggcggcc
    11161 gtgctctaca cagcgctcta cctgccgcgc cgcgctgagg ggcaggccgg ttgagtcggc
    11221 gatagcagtg cgggaggcgg cgcgccgctg ggcgcgccgc cgcccgcgcg gcgtcacgct
    11281 agatggtggg ctcggcctgc ggctcgcggt cgagctcgaa cgccgcgtgg agcgaccgca
    11341 ccgcaccggg gatctcccct cggctgatca tgcacgacac cttgatcggc gaggtcgaga
    11401 tcatctcgag gttgatcccc tggtcggcga gcgtgcggaa catcttggcc gccacgcccg
    11461 ggtgagatcg catgcccgca ccgacgaggg agaccttgcc gaggccgtga ttctcctcga
    11521 ccgacatggc gccgagctcg ctctgcgccg tttcgatcgc ggcacgggtc gtcggcacgt
    11581 cctcggccgg taccgagaac gagatctcgg ccttgccgtg gacgacgttc tgcatgatgg
    11641 tgtccacgtt gacgttggtc tgggcgatga cgtcgaagat cagtgccgcg acacccggtc
    11701 gatccgggat gtcgccgagc gtgaagacga cgtcggtctc gctgtgggta acagcggaga
    11761 cgatcggctg ttccatcctc gactcctcct cgatccacgt tcccggctcg tccgagaagg
    11821 tcgaccgagc gtggatgcgg actccgtggt tcctgccgag ctcgactgac ctgagcatca
    11881 gcaccttggc gcccgaggcc gacatctcga g
//
LOCUS       DBNGNK010000312         5285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_198274,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000312 DBNGNK010000000
VERSION     DBNGNK010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5285
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_198274"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..938)
                     /locus_tag="AC150V_09255"
     CDS             complement(105..938)
                     /locus_tag="AC150V_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763143.1"
                     /translation="MAAGDREEQEWRKRRRLVFWPLLFFVVLVGAMLPRALARGEPPP
                     TPTITGAPAAVATKTVAVFAFTGAEPFECALDDTAFVPCSSPVRYQGLDSGRHVFLVR
                     SRDGDGAARAAVHHWRIGAAPIPSSRPTQTAPRPAAPSPDIPPLPGTTVPVAETDGTF
                     SIRGTPAGPLAPGVARPLPLVVTNPNDFAIEVTSLTVTVAGGSSRPDCDGRVNVAVRQ
                     AGDGGSIRLTVSGGGSVQLPAQGVAAPIVRMRDLPSNQDSCKAARFSLLLDGAAVEAQ
                     P"
     gene            complement(942..1415)
                     /locus_tag="AC150V_09260"
     CDS             complement(942..1415)
                     /locus_tag="AC150V_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763144.1"
                     /translation="MRLAFASALIVLVAAVVVVPASGFWSLEGAFGLGSGDVETPAAV
                     VAEPATTTQALYPTGMAIGGVSLTLTNSNSIPVFVSQLALDAAQGTGGFAVDSAHAGC
                     DLTALTYTTQTNGGLGWTVPASSSLPLDLTNAISLSPAALDACQGASFTVYLKAG"
     gene            complement(1482..1946)
                     /locus_tag="AC150V_09265"
     CDS             complement(1482..1946)
                     /locus_tag="AC150V_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763145.1"
                     /translation="MHPDRKRRWLVLAILLVALAITAVAGYAFWTAGGSGTGSADVGT
                     TSSLVITQTSTVAGLRPGGPALTLSGDFDNPNAFSVRVNTISAALDAASLPVGCDPAW
                     FSIVGSPATNTQDVPSGTGVGAWSGISIQLTESGTNQDACKGATVSIDYSAT"
     gene            2400..>5285
                     /locus_tag="AC150V_09270"
     CDS             2400..>5285
                     /locus_tag="AC150V_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR3763146.1"
                     /translation="MIRWIVSSSVRLRVLVAAAAGALLVVGGFQLRDMRTDVLPEYGP
                     PIVEIQTEALGLSAEEVEQLITVPLEQDLLNGIAFLADIRSQSIPSLSRIILVFEPDT
                     DIFRARQVVAERLTQAHALPNVSKPPVMLQPQSSTNRVMIVAASSETVSPIELSVLAR
                     WTIVPRLVGVPGVANISVWGQRDRQLQVRVNPTRLRSQNVSLDDVVESVGNALWFSPL
                     TFVEASTPGTGGFIDTPNQRLGIHHVSPISTASDLAQVRIDETGNRALVLADVASVVE
                     DHQPLIGDAQATDGESLLFVVEKLPGADALDVTRGVESTIESFQLGLEGVEFDTTVYR
                     PASYLEEAIDNVTLLLILAAAVAAAAVCALFLRWRMAVITLVAIPIALVVATMVLQAL
                     GTTMNAIVLLGLTAAVSILIHDATVDVDSIARRLRRRGTERRSPESLVVEASSAVRLP
                     LVYATVAIALALTPLLFLNGVAGAFLPDVVAAYLVALAVSMLVALTLTPALSAILRPG
                     DPEDGGEPPLLRWLTAGYTRILSPVVRNPRLPLLAAGAVLLAAVATAPLLETSLLPRF
                     KETELLIRWNSPEGTSLPEMNRVTALASNELRSVAGVRNVGAHVGRAVTSDKAVGVNS
                     AELWVSIDRSADYESTIASVEAVVDGYPGFERQTATFANSRVDEVLTDIESDVVVRVY
                     GEELEILRGEAQKVRSAVAGVDGLDEVVVASPRVEPTVEIEVDLEAARRYGIKPGDVR
                     RTAATLVSGIQAGSLFEQQKVFDVVVWGTPETRGNLTDIRRLLIEAPNETYVRLEDVA
                     DVRVRPNPTVIERQAVSRYLDVQASLAGRNPTDVARDIERRVAGLAFPLGYHAEVLTQ
                     ERQNLWWVIGVAIGAAIGVLLLFHGLFDSWRLAALARAGAPIAGAGAVLGAIAAGGSL
                     SIASMIGLAAVCGLSLRFSSLSLDAFRTASPAEEEDAVRTFVRTAARP"
BASE COUNT          843 a         1780 c         1817 g          845 t
ORIGIN      
        1 ggcagttcct ccgctgccgg tcacgccggc gctccagtac gcgtacgcag gcactgcgac
       61 cgcgagcaac atcgcgatcg ccgcgacgag gagggcgcgc ggcctcacgg ctgcgcctcc
      121 accgcagccc cgtcgaggag cagcgagaag cgggcggcct tgcaggagtc ctggttcgag
      181 ggcaggtctc gcatgcgaac gatcggggct gccactccct gggcgggcag ctgcacgctg
      241 ccgccgccag agaccgtgag gcggatcgac ccgccgtcgc ccgcctgtcg tacggccacg
      301 ttcactcggc cgtcacagtc cggccgtgag cttcccccgg cgacggtgac ggtgagactc
      361 gtcacctcga tggcgaaatc gttcgggttc gtcacgacga gcgggagagg acgggcgacc
      421 ccaggtgcaa gcggcccggc cggagtgccg cggatcgaga acgtgccgtc ggtctcggcc
      481 acgggcacgg tcgtcccggg cagaggcggt atgtctggtg agggtgccgc tgggcgaggc
      541 gcggtctgcg tcgggcgcga ggaaggaatc ggggcggcgc cgatccgcca gtggtgcacg
      601 gccgcgcgcg cagcgccgtc cccgtcgcgg gagcgcacga ggaagacgtg gcgccccgag
      661 tccaggccct ggtaccgaac ggggctcgag cacggaacga aggcggtgtc atcgagcgcg
      721 cactcgaacg gctccgcccc cgtaaaggcg aagacggcga cggtcttcgt cgcaacggca
      781 gccggcgctc cggtgatcgt cggcgtaggc ggcggctcgc cccgcgcgag cgcgcgcggc
      841 agcatcgctc cgaccaggac gacgaagaac agcagcggcc agaaaacgag cctgcggcgc
      901 ttgcgccact cctgctcctc ccggtcaccg gcggccatgg tctagccggc tttcaggtag
      961 acggtgaagc tcgcgccctg gcacgcgtcg agagcggccg gcgagagcga gatcgcattc
     1021 gtcagatcga gcggcagcga gctggaagcc ggaacggtcc atccgaggcc tccgttcgtc
     1081 tgcgtcgtat aggtgagcgc ggtcagatcg cagcccgcgt gcgccgagtc gaccgcgaag
     1141 cctccggttc cctgcgcggc gtcgagcgcg agctgggaca cgaagacggg aatcgagttc
     1201 gaattggtca acgtcagcga gacgccgccg atcgccatgc ccgtcgggta cagggcctgg
     1261 gtcgtggtgg ccggctcggc gacgaccgcc gcgggcgtct ccacgtcccc ggagccgagg
     1321 ccgaaggcgc cttcgagact ccagaagccg ctggccggaa ccacaaccac cgcggcgacc
     1381 agaacgatga gagctgaggc gaatgccaga cgcatgccga ctcctcgacg tagagcgtgc
     1441 ggaggggcgg gagacgcccc tccgccgttg acatctgcga tctacgtcgc cgagtagtcg
     1501 atcgacaccg ttgcgccctt gcaggcgtcc tggttcgttc cggactcggt cagctggatg
     1561 ctgatgccgc tccaggctcc cacgcccgtg cccgagggca cgtcctgcgt gttggtcgcc
     1621 ggcgagccga cgatcgagaa ccacgccgga tcacagccga cgggcagcga tgccgcgtcg
     1681 agggctgcgc tgatggtgtt gacacgcacg ctgaacgcgt tcgggttgtc gaagtcgccg
     1741 ctcagagtga gagcgggtcc gccggggcga agcccagcga cggtgctcgt ctgggtgatg
     1801 accagcgagc tggtcgtgcc gacgtcggcc gacccggtgc cggagcctcc ggcagtccag
     1861 aatgcgtagc cggcaaccgc tgtgatcgcc agcgcgacga gcaggatggc aaggacgagc
     1921 cacctgcgct tccggtcggg atgcatgttc ctcctccaat gaggtaggga cggacaggta
     1981 cgtcccggcc tatcggcgcg gagaggatca ctcttgagag ggatggagcc gctccgggac
     2041 aagatccccc gatcgaggga gtcgcctcgg atcgttcgtt ccgccagtcg cgaaacgggg
     2101 ctgagtggcc agccgtggga gatcacgagt cagcggctcg ccgtgctaat cccaccattg
     2161 acaacgcgtc ggcatcttgg cgaccatggt cgaggttggc gaacatcggt cgaggtacgc
     2221 ggcgggggct ccgggagaga agacgtccca tctgcgccgc gtcgagcggg ggtggggaga
     2281 gttagcgagg cgacaaaacg ctgcagctgt agtgtccgag cgaccaaacc gtcagccacg
     2341 gcgggccgca tgcccgccgg ctttccgccg gcgcgtccac tccggccaga gctgaacgcg
     2401 tgatccgctg gatcgtctcg tcgagcgtcc gactgcgcgt gctcgtcgcg gcggcggccg
     2461 gagccctgct cgtcgtgggg ggcttccagc tcagagacat gcggaccgac gtgttgcccg
     2521 agtacggacc gccgatcgtc gagatccaga ccgaggccct ggggctctcg gcggaagagg
     2581 tcgagcagct gatcacggta ccgctcgagc aggacctgct caacgggatc gccttccttg
     2641 ctgacatccg ctctcagtcg attccgagtc tttcccggat catcctcgtc ttcgagccgg
     2701 acaccgacat cttccgagcc agacaggtgg tggccgagcg cctgacccaa gcgcacgccc
     2761 tgccgaacgt gtcgaagccg ccggtgatgc ttcaacctca gtcgtcgacg aaccgggtca
     2821 tgatcgtcgc cgcttcgtca gagaccgtct ccccgatcga gctctcggtg ctcgcgagat
     2881 ggacgatcgt cccgcggctc gtcggtgtac ctggagtagc gaacatctca gtctgggggc
     2941 aacgcgaccg gcagttgcag gtgcgagtga acccgacgcg cctgcgcagc cagaacgtgt
     3001 cgctcgatga cgtcgtggaa tcggtcggca atgcgctctg gttctcgccg ttgacattcg
     3061 tcgaggcctc gacgccggga accggcggct tcatcgacac accgaaccag cggctcggga
     3121 tccaccacgt gtctccgatc agcactgcat ccgatctcgc gcaggtgcgc atcgacgaga
     3181 caggcaaccg cgccctcgtg ctcgccgatg tcgcgtcggt cgtggaagat caccagcccc
     3241 tgatcgggga cgcccaggct actgacggag aaagccttct cttcgtcgtc gagaagctgc
     3301 cgggcgccga tgccctagac gtcactcggg gcgtcgagtc cacgatcgag tcattccagc
     3361 tcggtctcga gggagtcgag ttcgacacga ccgtctaccg cccggcgtcg tacttggagg
     3421 aggcgatcga caacgtcacc ctcctgctga ttctcgccgc cgcagtcgcg gccgccgcgg
     3481 tgtgtgcgct gttcctgaga tggcgcatgg ccgtcatcac gcttgtcgca attccgatcg
     3541 ccctcgtcgt cgcgacgatg gtgcttcagg cgctcggaac gacgatgaac gcgatcgtcc
     3601 tcttggggct caccgccgcg gtgagcatcc tcatacacga cgccaccgtc gacgtcgaca
     3661 gcatcgcgcg ccgccttcgc cggcgaggca ccgagcggag atcgcccgaa agcctcgtag
     3721 tcgaggcctc gtcggcggtc aggctgccgc tggtctacgc gacggtcgcg atcgcgctgg
     3781 ccctcacacc gctgctgttc ctcaacggtg tcgcgggtgc gttcctcccg gatgtggttg
     3841 ccgcctacct cgtggcgttg gctgtctcga tgctcgttgc gctgacactg accccggcgc
     3901 tcagcgccat ccttcgcccc ggcgatcccg aagacggggg cgagccccca ctcctgcgat
     3961 ggctcacggc tggctacaca cgcattctct ctccggttgt ccgcaatccg cggttgccgc
     4021 tactcgccgc cggcgccgtg ctgctggctg cggtcgctac cgccccgctc ctcgagacgt
     4081 cgctgctgcc gaggttcaag gagaccgagc tcctgattcg ctggaacagt ccggagggca
     4141 cgtccctgcc cgagatgaac cgggtcaccg ctctcgccag caacgagctg cgctctgtgg
     4201 ccggcgtccg caacgtcggc gcgcacgtcg gccgcgcagt gacctcggac aaggcggtcg
     4261 gcgtgaactc tgccgagctc tgggtgagca tcgatcgatc tgcggactac gaatcgacca
     4321 tcgcgtccgt cgaggcggtc gtcgacggct atcccggatt cgagcgtcag accgcgacct
     4381 tcgccaacag ccgagtcgac gaagtcctga cagatatcga gagcgacgtc gtcgtgcgcg
     4441 tctacggaga ggagctggag atcctccgcg gggaggcgca gaaggtgaga tccgctgtcg
     4501 caggtgtcga cggtctcgac gaggtggtcg tggcctcacc ccgtgtcgag ccgacggtgg
     4561 agatcgaggt cgatctcgaa gccgcccgcc ggtacggcat caagccgggt gacgttcgga
     4621 gaacggcagc gacgctcgtc tccgggatcc aagcgggcag cctcttcgag cagcagaagg
     4681 tgttcgacgt cgtcgtctgg ggaacaccgg aaacccgtgg gaacctcacc gacatccgac
     4741 ggctgctgat cgaggccccg aacgagacgt acgttcggct cgaagacgtc gcagacgttc
     4801 gcgtcaggcc caatccaacg gtgatcgagc gccaggctgt ctcacgctat ctcgatgtcc
     4861 aggcaagcct ggctgggcgc aatcccacgg acgtagcccg tgatatcgag cgtcgcgtgg
     4921 ccgggctggc gttcccgctc ggatatcacg ctgaggtgtt gacgcaggag cggcagaacc
     4981 tctggtgggt gatcggcgtg gcgatcggcg ccgcaatcgg cgtcctcctg ctgttccacg
     5041 ggctgttcga cagctggcgc ctcgccgccc ttgcgcgggc gggcgctccg atcgccggcg
     5101 cgggcgccgt cctcggcgca attgccgcgg gcggctcgct ctcgatcgcc tccatgatcg
     5161 gcctcgccgc cgtgtgcggg ctctccctcc gcttctcgag cctgtcgctc gatgccttcc
     5221 gaacggcgag tcccgccgaa gaagaagacg cagtccgaac ctttgttcgg acggctgcca
     5281 ggccg
//
LOCUS       DBNGNK010000313         6023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1846669,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000313 DBNGNK010000000
VERSION     DBNGNK010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1846669"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="AC150V_09275"
     CDS             complement(<1..122)
                     /locus_tag="AC150V_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HIR3763147.1"
                     /translation="MGEAIGDILPNAVGVAISPVPIIAVILMLFTKRARSNSLA"
     gene            213..1985
                     /locus_tag="AC150V_09280"
     CDS             213..1985
                     /locus_tag="AC150V_09280"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR3763148.1"
                     /translation="MSEPSAAPRKTSGNSPAYDRVRGMLARDEPVILDGAVATELERL
                     AQAGGELKNADDSGLWGTHALYHNPYAVLDVHRSYVAADIDVLTTDTWAILAAAELEA
                     GGLVGRPGTTHWMDIARLALRLARQAIDEADKTGEVAIAFSVNGDVDSEERRQTLELL
                     LRVLEDDPPDLLFLETMQLVREPLTDETVETALSTGLPVWLSFRRCRHGVCGVYGQHW
                     GGPEGDLFGRSARRFEELGVAALLVNCLPPSHIEGMLPWLRDFVDLPLGAYPNLGHYS
                     DAGWRFDERVGPGEFAELAAAWRGEGAQIIGGCCGTTPEHIAALRPRLADTKAGRPRS
                     VPAEIDREDAPGPPAEPWLDDAGRRLYPIPFPEIIREPDVFVPTQGSLLVWKHLWREG
                     VGPGKRCLDVGCGSGLLAIQLALNGADRVHGIDIQRQAVANTLANAFRNGVSDRVTGE
                     EADIYTYEPAERFDVVVASLYQMPVDPFEEYTGHRPLDYWGRNLFDHFTDLLPRLLAD
                     DGTAYVMQLSILSQVRTEELVRKAELSVRVLDLSFFPFTSVFEGNREQIERVEQLSDA
                     YHLTLGGDDYVVAYLLELTRSPNA"
     gene            2015..2080
                     /locus_tag="AC150V_09285"
                     /pseudo
     CDS             2015..2080
                     /locus_tag="AC150V_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024637.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            2229..2678
                     /locus_tag="AC150V_09290"
     CDS             2229..2678
                     /locus_tag="AC150V_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763149.1"
                     /translation="MVAGAQARTGHPDAEDVFGEWIEFARKVFAGNFAEVSHWIVDAS
                     FAAVLLAREEEVVEVLPEVPTRWHVAAIAVLRKDAMGAADVLAEIGCPGYEAEARAVA
                     ARSLAGKGHVAAADEQAGLACALYTGLSASAAIARLDAVLENATATG"
     gene            2789..3268
                     /locus_tag="AC150V_09295"
     CDS             2789..3268
                     /locus_tag="AC150V_09295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3763150.1"
                     /translation="MIPVSGIHHFSITCADADRSAAFYRDLFGLEVMSDREIPPGGFT
                     ERVTGVDGARVRIVHLHGHGLNFELLEYKEPRGDVRAREPNHAGSAHLCFVTDDMEAS
                     VAALRRAGVPFRSADGDAHKVDGGPNDGGSCVYVEDPDGNAVELVHLVRPWLGKGDS"
     gene            3268..4011
                     /locus_tag="AC150V_09300"
     CDS             3268..4011
                     /locus_tag="AC150V_09300"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3763151.1"
                     /translation="MDTGLAGRTAIVTGGARGIGLACAAALAAEGAKLVLADVDGPAA
                     EASADTLPGDAFGLTVDVTDPDGVNGMVDEAVSRHGGLDVLVTSAGIFHATPFDEITV
                     EEWDRIQAVNLKGTFLCAQAALRVMGPRQRGRIVTIASLAAQTGGLAAGASYAASKGG
                     VLSLTKSIARYAGPYGITANSINPGVIDSAMIASWPPGRQEATTEATSLQRIGTTAEV
                     AASVVWLASDAAAFVHGTHLDVNGGLLMD"
     gene            4033..4953
                     /locus_tag="AC150V_09305"
     CDS             4033..4953
                     /locus_tag="AC150V_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3763152.1"
                     /translation="MAQAKVCRGTWCMAFGFEPKMATMEQVVPVLAAFGYDGVELGGF
                     FDHCTVERFPDKASRTELKKWINDQGLEIAGIAPGPYGDLFRLPWATGSKEVYDEYIE
                     YFEGYLQLAADMEIPGMRVDPGAAGPLPYDADYDEIWDRVIKTFQHHAERGQEYGVAM
                     LWELESLQPFNKPSETVKILNDVGNPNFKLMYDTGHFQACGVIGHNHVQPAEQLPNQI
                     EFIKMLPAGSIGHMHMCDTDMNTHFNMFGTKSNFGEGIIDFDELMPVLAGAYDGEWWA
                     VDSIPMGSKAWQDTWDGLITLNDLLDKYVR"
     gene            4977..>6023
                     /locus_tag="AC150V_09310"
     CDS             4977..>6023
                     /locus_tag="AC150V_09310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR3763153.1"
                     /translation="MKAAVFYEAEDLRLEDVSEPEAGADEVVVKVLACGFCGSDIEYY
                     YGKSPVGTPDGKGPLVLGHEICGEVVQAGKLAEQYGLAEGDRVALNPVQSCNACNNCR
                     SGKLQFCENLTVLGVTMNGGFAEYVKTKIAHAYKLPEGLTDEQGAFVEMLSSAVYAVK
                     RAEIEPENFAVVWGPGPVGLSMVQLLKNEGARVGVAGTRDYRLDLAKTVGADFVWNVS
                     DESSSNYVDDLATAVQEANGGALADRALVATASVPANQQAVDVTGPGSTVVLMGLAGP
                     DDQISVKLLSNLYMDKTIRFSLLYPLQWPTTIKILEDAKVDTSEIITHSSDLDGIAPA
                     IERVLNREDDVIKTV"
BASE COUNT         1013 a         2065 c         2040 g          905 t
ORIGIN      
        1 aaggcaaggc tgttgctgcg cgcccgcttc gtgaagagca tgaggatcac ggcgatgatc
       61 ggaacgggac tgatcgccac tccgaccgcg ttcgggagga tgtcgccgat cgcctcgccc
      121 atgccgctgt cctttcgccg cgcccggcgg cgcaagctat cgcgcgcgga gtgaagccgc
      181 aatcacgggg tacgccgccg tacactcagc acgtgtccga gccgtcggcc gcgcctcgca
      241 agaccagcgg caactccccc gcgtacgacc gtgttcgcgg aatgctcgcg cgtgacgagc
      301 cggtgatcct cgacggcgcc gtggccacag agctggagcg gctcgcacaa gccggcggcg
      361 agctgaagaa cgccgacgac agcggcctct ggggaacgca cgctctctac cacaacccct
      421 acgccgtgct cgacgtccac cgcagctacg tcgccgccga catcgacgtc ctcaccaccg
      481 acacgtgggc gatcctcgcc gccgccgagc tggaggcggg cggcctggtc ggccggcccg
      541 gaaccacgca ctggatggac atcgcgcgcc tcgcgctccg cctcgcccgc caggcgatcg
      601 acgaggcaga caagacgggt gaggtcgcaa tcgccttctc ggtcaacggc gacgtcgaca
      661 gtgaggagcg acggcagacg ctcgagctgc tcctgcgggt gctcgaagac gacccgcccg
      721 acctgctctt cctcgagacg atgcagctcg tgcgcgagcc gctcacggac gagacggtcg
      781 agacggcgct ctcgacgggt cttcccgtct ggttgagctt caggcgctgc cgccacggcg
      841 tgtgcggcgt ctacggccag cactggggcg ggccggaggg cgatctcttc ggccgctcgg
      901 cccgcaggtt cgaggagctc ggcgtcgccg ccctgctcgt caactgtctt cccccgtcgc
      961 acatcgaggg catgctgccc tggctccgcg acttcgtcga tttgccgctc ggcgcctacc
     1021 cgaacctcgg ccactactcc gacgccggct ggcgcttcga cgagcgcgtc ggaccgggcg
     1081 agttcgccga actcgcggcg gcgtggaggg gcgaaggagc gcagatcatc ggcggctgct
     1141 gcggcacgac acccgagcac atcgccgcgc tacgaccccg gcttgccgac acgaaggccg
     1201 gaaggccacg ttccgtcccc gcggagatcg accgcgagga cgcgccgggc ccgcccgccg
     1261 agccgtggct cgacgacgcc ggccggcgcc tgtatccgat cccgttcccg gagatcatcc
     1321 gcgagcctga cgtcttcgtc ccgacgcagg ggagcctgct ggtctggaag cacctctggc
     1381 gggagggagt cggaccggga aagcgctgcc tcgacgtcgg ctgcggctcc gggctgctcg
     1441 cgatccagct cgccctgaac ggcgccgacc gggtgcacgg aatcgacatc cagcgccagg
     1501 cggtcgcgaa cacgctggcg aacgccttcc gaaacggcgt ctcggaccgg gtcaccggcg
     1561 aggaggcgga catctacacg tacgagccgg cagagcgctt cgacgtcgtc gtggcaagcc
     1621 tgtaccagat gccggtcgac ccgttcgagg agtacacggg acatcgaccg ctcgactact
     1681 ggggccggaa tctcttcgac cacttcaccg acctgctacc ccggctgctc gcagacgacg
     1741 gcacggccta cgtgatgcag ctgtcgatcc tctcgcaggt ccgcacggag gagctcgtcc
     1801 gcaaggccga gctctcggtg cgggtgctgg atctttcctt cttccccttc acctcggtgt
     1861 tcgagggcaa cagggagcag atcgagcgtg tcgagcagct ttccgacgcc taccacctga
     1921 ccctcggcgg cgacgactac gtcgtcgcgt acctgctcga gctgacccgc tctccgaacg
     1981 cctgagcgaa cgaccgagcc gccgtagact ccgcgtgctc gtctgcagct cctgcgggca
     2041 ggagaacccg gccggatcgc ggttctgcaa cgcctgcggg ctctccgccg tcgtcgacgc
     2101 cgacccggag ctcatcttct ggcgccaggg ccggtcgctc ccttacggcg agggggtcgc
     2161 attctgggcg ctcggcgaga tcgtgaaggc ccaggcgggc attctcgagt ccgacgacgg
     2221 ggcttgggat ggtggccggt gctcaagcac gtaccggcca tcccgatgcc gaggacgtct
     2281 tcggcgagtg gatcgagttc gcgcgcaagg tgttcgcggg caacttcgcc gaggtgtcgc
     2341 actggatcgt cgacgcctcg ttcgcggcgg tactgctcgc tcgcgaggag gaagtcgtcg
     2401 aagtcctacc cgaggtcccg acgcgctggc acgtggccgc aatcgccgtc ctccgcaagg
     2461 atgcgatggg cgcagcggac gtgttggcgg agatcggctg ccccgggtac gaagccgagg
     2521 cgcgggcggt cgccgcccgc tcgcttgccg ggaagggaca cgtcgcggct gccgatgagc
     2581 aggccggact ggcgtgcgcc ctctacacag gactcagcgc ctcggccgcg atcgcccgcc
     2641 tcgacgccgt gctcgagaac gccaccgcca ccggctgacc cgatccgcgc aggcgcgaca
     2701 gttgacgctg caaaccgtct gccactcgtg tagaaaacgt ttacagggac gtccccttcc
     2761 ccgggatcga aggaggagcc gaggcagcgt gatccccgtc agcggcatcc accacttctc
     2821 gatcacgtgc gccgacgccg atcggtcggc ggcgttctac cgggatctgt tcgggctgga
     2881 ggtgatgtcc gacagagaga ttccgcccgg cggattcacc gagcgcgtca cgggcgtcga
     2941 cggcgcccga gtgcggatcg tccacttgca cggccacggc ctcaacttcg agctgctcga
     3001 gtacaaggag ccgcgcggcg acgtcagagc ccgcgagccc aaccacgcgg gcagcgccca
     3061 tctctgcttc gtgaccgacg acatggaagc gtcggtcgct gcgctccgcc gggccggcgt
     3121 cccgttccgc tccgcggacg gggacgccca caaggtggac ggaggcccca acgacggcgg
     3181 cagctgcgtc tacgtcgagg atcccgacgg caatgcggtc gagctcgtgc accttgtccg
     3241 gccctggctc gggaagggcg actcgtgatg gatacgggtc tcgccggcag gaccgcgatc
     3301 gtcaccggcg gagcccgcgg catcgggctc gcctgcgcgg ccgcgctcgc ggctgagggc
     3361 gcgaagctcg tcttggccga cgtggacggc ccggccgcgg aagcctccgc cgacacgctc
     3421 ccaggcgacg ccttcggtct caccgtcgac gtgaccgacc cggacggcgt gaacgggatg
     3481 gtcgacgagg ccgtgtcccg acacggtggc ctcgacgtcc tcgtcacctc ggccggcatc
     3541 ttccatgcga caccattcga cgagatcacc gtcgaagagt gggaccggat ccaggccgtc
     3601 aacctgaaag gaactttcct ctgcgcccag gcggcgctgc gggtgatggg tccgcggcaa
     3661 cgcggacgga tagtgaccat cgcatctctc gcggcccaga cgggcgggct cgcggccggc
     3721 gcgtcgtacg cggcgtcgaa gggaggcgtg ctctcgctca ccaagtcgat cgcccgctac
     3781 gccgggccgt acggcatcac cgcgaacagc atcaaccccg gggtgatcga ttcggcgatg
     3841 atcgcctcgt ggccccccgg gcgacaggag gcgaccaccg aagcgacctc cctacagcga
     3901 atcggcacta ccgccgaggt ggctgcctca gtggtctggc tcgcctccga cgctgccgct
     3961 ttcgtccacg gcacgcacct ggacgtcaac ggcggactgc tcatggacta gttgcgcgaa
     4021 aggagaaaga agatggcaca agcaaaggtt tgccgaggca cgtggtgcat ggccttcggc
     4081 ttcgagccga agatggccac gatggagcag gtcgtcccgg tgctcgccgc attcggctat
     4141 gacggcgtcg agctgggcgg cttcttcgac cactgcacgg tcgagcgctt cccggacaag
     4201 gcgagccgga cggagctgaa gaagtggatc aacgaccagg ggctggagat agcgggcatc
     4261 gcgccgggcc cgtacggcga cctgttccgg ctgccgtggg cgacgggctc caaagaggtt
     4321 tacgacgagt acatcgagta cttcgagggc tacctgcagc tcgcggccga catggagata
     4381 cccggcatgc gcgtggatcc cggtgccgcc ggcccgttgc cgtacgacgc ggactacgac
     4441 gagatctggg accgcgtcat caagacgttc cagcaccacg ccgagcgcgg tcaggagtac
     4501 ggcgtggcca tgctctggga gctggagtcc ctgcagccgt tcaacaagcc gtcggagacg
     4561 gtgaagatcc tgaacgacgt cgggaacccc aacttcaagc tgatgtacga cacgggccac
     4621 ttccaggcct gcggcgtgat cggccacaac cacgtccagc ccgcagagca gctgccgaat
     4681 cagatcgagt tcatcaagat gctcccggcc gggtcgatcg gccacatgca catgtgcgac
     4741 accgacatga atacgcactt caacatgttc gggacgaagt cgaacttcgg cgagggcatc
     4801 atcgacttcg acgagctgat gcccgttctc gccggcgcct acgacggcga gtggtgggcc
     4861 gtagactcga tcccgatggg ctcgaaggcc tggcaggaca cgtgggacgg cctcatcacg
     4921 ctcaacgacc tgctcgacaa gtacgtccgc tagcgcggac ggctaggagg cacaccatga
     4981 aagcagcagt tttctacgag gccgaggacc tccggctcga ggacgtctcg gagccggagg
     5041 ccggcgccga cgaggtcgtc gtcaaggtgc tggcatgcgg gttctgcggc tcggacatcg
     5101 agtactacta cggcaagagc cccgtcggga cgcccgacgg caaggggccg ctcgtcctcg
     5161 gacacgagat ttgcggagag gtcgtccagg cggggaaact ggctgagcag tacgggttgg
     5221 ccgagggcga ccgcgtcgcc ctcaacccgg ttcagagctg caacgcctgc aacaactgcc
     5281 gctccggcaa gctgcagttc tgcgagaacc tcaccgtgct cggcgtgacg atgaacggcg
     5341 gcttcgccga gtacgtgaag acgaagatcg cgcacgcgta caagctgccc gaggggctga
     5401 cggacgagca gggggcgttc gtcgagatgc tctcgtcggc cgtctacgcg gtgaagcggg
     5461 ccgagatcga gcccgagaac ttcgcggtcg tctggggccc cggcccggtc ggtctctcga
     5521 tggtgcagct cctcaagaac gagggtgcca gggtcggtgt ggccggcacg cgggactacc
     5581 gcctcgacct cgcgaagacc gtgggcgcgg acttcgtctg gaacgtctcc gacgagtcgt
     5641 cgtcgaacta cgtcgacgac ctcgcaactg cggtgcaaga ggcgaacggc ggcgcgctcg
     5701 ccgaccgcgc gctggtggca accgcctcgg tgccggcgaa ccagcaggcc gtcgacgtga
     5761 cagggcccgg ctcgacggtc gtcctgatgg ggctcgctgg gccggacgac cagatcagcg
     5821 tgaagctgct ctccaacctc tacatggaca agacgatccg gttctcgctg ctctacccgc
     5881 tgcagtggcc gacgaccatc aagatcctgg aggatgccaa ggtcgacacc tccgagatca
     5941 tcacccactc gtccgacctc gacggcatcg cgcctgcaat cgaacgcgtg ctgaaccgcg
     6001 aggacgacgt gatcaagacc gtc
//
LOCUS       DBNGNK010000314         3564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1486917,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000314 DBNGNK010000000
VERSION     DBNGNK010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1486917"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="AC150V_09315"
     CDS             <1..387
                     /locus_tag="AC150V_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR3763154.1"
                     /translation="AVGIGAGAPRLGANDNFSASVRFGDGSVCTLTYTALGHRDHPKE
                     RLEVFADAMVLSLDDYRALTVSGAKSGGWKSTTQQKGHREELEALADALRDGGLWPIS
                     LAEQLAATRISFAVEERLRTPAQRLE"
     gene            384..1202
                     /locus_tag="AC150V_09320"
     CDS             384..1202
                     /locus_tag="AC150V_09320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763155.1"
                     /translation="MTETRHLDALVESMHGHRKKTLFFIDCLEQHAAASGRTPDQLSV
                     LELGCGNGTVVALPVAEQGFDVTGVDIHEPSIAAARESNDLANARFLTADAAAFEADR
                     PFDAVILSDVLEHVQNPHELLRHAVAQLQANGLVLISVPNGRGPYELEQRLVADGPFR
                     PLVRIAHEGLVSAARVKRALQRKPWPPPEEALPAYNYDSPHVQFFTRASLAALLADSK
                     LEVVRTGNGAWVGGDLTYHVFYAAPRLIDVSLAIADRLPARFVSSWHVACRRAS"
     gene            1212..2069
                     /locus_tag="AC150V_09325"
     CDS             1212..2069
                     /locus_tag="AC150V_09325"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763156.1"
                     /translation="MADVLAGEKQAAIEQWTADPCGASVAEGTLGTAAYFRSLVQARS
                     EYAPWMDEKLDYRGSAGLDVLDVGCGQGIDLFHYAVAGARPTGIDLTPRHVELSRAHL
                     SAVGLEATVAEGDAENLPFPDASFDRVSSNGVLHHTPDMPAALRELVRVLRPGGEARI
                     IVYNKSSFHYWLTQVLYIGLLRGALFRERSMAGVLSSGVEYSTIGARPLVRVYTRSRL
                     ASLLREAGFDGVRVSARHFHLSDTPFTYPLRRFRRLRNAEILDAVGRVGGWYLVGTGR
                     KPRANPAVR"
     gene            2169..>3564
                     /locus_tag="AC150V_09330"
     CDS             2169..>3564
                     /locus_tag="AC150V_09330"
                     /inference="COORDINATES: protein motif:HMM:NF028199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR3763157.1"
                     /translation="MLARRVADEARSEFERIAGPRRARRFDEAALLRATQAPSLTLLW
                     DEIHARPFPAVTEPARARAVLRRFPGEEDRVTALADAAIARRVDLLGSGPTELGKPVD
                     WLRDFKTGLSWPKGDVRAVDYADLSRPSDVKVPWELSRMQWLVPAAQAYLVTGDERYP
                     HEARELLEEWIAANPYGRTVNWAIAMESAMRILTWTYFFHCFGASEAWSAPDFRSGFL
                     RCLWLNAEFTARNLERSDVNGNHYDANAAGLVFAGLFFPGRRAEQWAATGWEMLVAEL
                     PRQVTPDGVDFEMATSYHRLVCELFLLPALYRSCFGLSVPDWYAERLGAMALFAAAYT
                     RSDGSSPFWGDADDARALPLGDQAVGDHRYLAGLVGIGLERPETLDCAAGPRTEAAWL
                     LGADAAATLPEREAQPIGSQGFEEGGVYVMRGLADHVFVDCGPVGLSGRGGHGHNDCL
                     SVDAVLAGAHLLTDC"
BASE COUNT          513 a         1266 c         1222 g          563 t
ORIGIN      
        1 gccgtgggga tcggcgcggg cgcgccgcgg ctcggcgcca acgacaactt ctccgcgagc
       61 gtgcgcttcg gcgacggctc ggtgtgcacg ctcacgtaca cggctctcgg ccatcgcgac
      121 catcccaagg agcggctgga agtcttcgcg gacgcgatgg tgctctcgct cgacgactat
      181 cgcgcgctta cggtctcggg cgccaagtcc ggcggttgga agtcgacgac ccagcagaag
      241 ggccaccgtg aagagctgga ggcgctcgcc gacgcgcttc gcgacggcgg gctatggccg
      301 atctcgctcg ccgagcagct cgcggcgacc cgcatcagct tcgcggtcga ggagcggctg
      361 cgcactccgg cgcagaggct cgagtgacgg agacgcgaca cctggatgca ctggtggagt
      421 cgatgcacgg gcaccgaaag aagacgctgt tcttcatcga ctgcctcgag cagcacgcag
      481 ccgcatcggg ccggacgccg gaccagctct ccgtgctcga gctgggctgc ggaaacggca
      541 ccgtcgtggc cctgccggtc gcggaacagg gcttcgacgt caccggagtc gacatccacg
      601 agccgtcgat cgcggcggct cgcgagagca acgatctcgc caacgcccgt ttcctcacgg
      661 ccgacgccgc cgccttcgag gcggacaggc ccttcgacgc ggtgatcctg tcggacgtcc
      721 tcgagcacgt ccagaacccg cacgagctcc ttcgacatgc ggtcgcccag ctgcaggcga
      781 acggcctcgt cctgatctcg gtgccgaacg ggcgagggcc ctacgagctc gagcagcgtc
      841 tcgtcgcgga cgggccgttc cggccgctcg tccggatcgc gcacgaaggg ctggtctcgg
      901 cggcccgcgt gaagcgggcg cttcagcgca agccgtggcc gccccccgaa gaggcgctcc
      961 ccgcctacaa ctacgactcg cctcacgtcc agttcttcac gcgggcgtcg ctcgccgccc
     1021 ttctggccga cagcaagctc gaggtcgtcc gcaccggcaa cggcgcctgg gtcggaggcg
     1081 acctgacgta ccacgtcttc tatgccgcac cccggctgat cgacgtttcg ctcgcaatcg
     1141 cggaccggct gcccgcccgg tttgtgtcga gctggcacgt cgcctgccgc cgggcgagct
     1201 agcatcgaca gatggccgac gttctcgccg gcgaaaagca ggccgcaata gagcagtgga
     1261 ccgccgatcc ctgcggagcg agcgttgcgg aaggcaccct cgggacggcc gcctacttcc
     1321 gaagcctcgt ccaggcgcgc tccgagtacg caccctggat ggacgagaag ctcgactacc
     1381 gaggctctgc cgggctcgac gtgctcgacg tcggctgcgg gcagggcatc gacctcttcc
     1441 actacgccgt cgcaggagcg cgaccgaccg gcatcgacct cacgccgcgg cacgtcgagc
     1501 tgtcccgtgc gcacttgtcc gccgtcggac tcgaggccac ggtcgccgag ggcgacgcgg
     1561 agaacctgcc gtttccggac gcgtcgttcg atcgcgtatc gagcaacggc gtcctgcacc
     1621 acacgcccga catgcccgcc gcgttgcgag agctggttcg cgttctccga cccggcggcg
     1681 aggcgcgcat catcgtctac aacaagagct cgttccacta ctggctgacg caggtcctgt
     1741 acatcgggct gctgcgcggc gccttgttcc gggagcgctc gatggccggt gtcctctcga
     1801 gcggagtcga atactcgacg atcggagccc ggccgctggt ccgggtgtac acgcgctcgc
     1861 ggctcgcctc gctcttgcgc gaagccggtt tcgacggcgt gcgcgtcagc gcgcggcact
     1921 tccacctcag cgacactccc ttcacctacc cgctgcggcg tttccgtcgc cttcgtaacg
     1981 cggagatcct cgacgcggtc ggtcgggtcg gcggctggta tctggtcggc acggggcgga
     2041 agccgagggc caacccggcc gtgcggtagg ccgacgcggc acgagaccac ttctggcccg
     2101 tgctaggctg gcccgccgtg gcgcggcacg cctcaagagc tcgccgcgcg ctgcgcatgc
     2161 ccccacgcgt gctcgcgcgg agggtcgccg acgaggcgcg atccgagttc gagcgaatcg
     2221 ccgggccccg ccgcgcgcga cgtttcgacg aggccgcact cctgcgtgcg acccaagcgc
     2281 cgtcactaac gctcctctgg gacgagatcc acgctcggcc gttccctgca gtgacggagc
     2341 ccgcgcgtgc gcgcgcagtt ctccggcggt tccctggtga ggaggatcgg gtcactgctc
     2401 tcgccgacgc cgcgatcgcg cgccgcgtcg acctcctcgg aagcggcccg acggagctcg
     2461 ggaaacccgt cgactggctg cgtgacttca agacgggact gagctggccg aagggggacg
     2521 ttcgcgccgt cgactacgcg gacctctcgc ggccgagcga cgtcaaggtt ccgtgggagc
     2581 tctcgcgtat gcagtggctc gttccggccg cccaggcgta tctcgtcacc ggtgacgagc
     2641 gctatccgca cgaggcacgg gaactgctcg aggagtggat cgccgccaat ccgtacggtc
     2701 gcaccgtcaa ctgggcgatc gcaatggagt cggcgatgcg aatcctcacc tggacgtact
     2761 tcttccattg cttcggagcc agtgaagcct ggtcggcacc cgacttccga agcggcttcc
     2821 tgcgctgcct ctggctcaat gccgagttca cggctcggaa cctcgagcgc tcggacgtga
     2881 acgggaacca ctacgacgcg aacgccgccg gactggtgtt cgccggcctc ttcttcccgg
     2941 gacggcgcgc cgagcaatgg gcggcgaccg gctgggagat gctcgtcgcg gaattgccgc
     3001 ggcaggtgac gccggacggc gtcgacttcg agatggcgac gtcgtaccac cggctcgtct
     3061 gcgagctgtt cttgctgccg gccctctacc gctcctgctt cgggctttcg gttcccgact
     3121 ggtacgccga gcgcctgggg gcgatggcac tgttcgcggc cgcgtacacc cgctccgacg
     3181 gctcgtcgcc gttctggggc gacgcggacg acgcgcgcgc gctgccactc ggcgatcaag
     3241 ccgtcgggga tcaccgctat ctggcaggtc tggtcggaat cggcctcgag cggcccgaga
     3301 cgctggattg tgcggccggc cctcgtactg aggcagcgtg gttgctcggc gccgacgcgg
     3361 cggcgacgct gccggagcgc gaggcccagc cgatcggctc tcagggattc gaggagggcg
     3421 gcgtctacgt catgcgaggg ctggccgatc acgtcttcgt ggactgcggt ccggtcggcc
     3481 tgtccggacg gggaggacac ggccacaacg actgtctctc ggtcgacgcc gtgctcgccg
     3541 gcgcccacct gctgaccgac tgcg
//
LOCUS       DBNGNK010000315         4179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1989866,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000315 DBNGNK010000000
VERSION     DBNGNK010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1989866"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="AC150V_09335"
     CDS             complement(<1..943)
                     /locus_tag="AC150V_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR3763158.1"
                     /translation="MPTAVREYVRALELDAYVVGGAVRDELLGIPHKDEDFLVPDMDH
                     AALRAALARHGRVDDMEVHGQIVGVRFQPDDRDVAALAPTGIELAPARAARSTGPGHR
                     DFSIVADGTVSIVEDMERRDFTVNAMALRLTTGELVDPFGGLAALGRRELRTVTPHSF
                     SEDPLRMLRALRLVSQLAFDLAGETLAQMRLHAARIRHVAPERIGGGLAADGHGELSR
                     LLLGSEPARALAIARDTGVLSVVIPEFAPAIGYELASPRQPFPLDGHILATVQGAADI
                     GASLPVRLAALLHDLGKPTATAPTSHAAEGARMAGTIL"
     gene            complement(1005..1388)
                     /locus_tag="AC150V_09340"
     CDS             complement(1005..1388)
                     /locus_tag="AC150V_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR3763159.1"
                     /translation="MEKTVVRTEAAPAPFQGAPYNQAITTDDLVFVAGQLGLDTSGTM
                     VGPGVVEQTEQVMRNIAAILEAAGSSINAVVKTTVFLVDLADFDAMNEVYARHVGDRP
                     PARSTIGVAALPAGGRVEIEAVAHR"
     gene            complement(1400..1888)
                     /locus_tag="AC150V_09345"
     CDS             complement(1400..1888)
                     /locus_tag="AC150V_09345"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012687.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HIR3763160.1"
                     /translation="MSGARVAAGSGVSNVSTGVSVLDHLLGLLAHAGKFDLSLEIEPD
                     DPEAEVDAAGDALGTAIAPLLTEGAHGFGTVPADEALATVAVEASGRPLVASNVDLGP
                     SRVGGLDTDLAARFLRGLADTTGLTIHVRLIEGEDTDHVLESIFKSLGVALAQAATAG
                     ER"
     gene            complement(1937..3217)
                     /gene="murA"
                     /locus_tag="AC150V_09350"
     CDS             complement(1937..3217)
                     /gene="murA"
                     /locus_tag="AC150V_09350"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR3763161.1"
                     /translation="MQSFVIEGGRPLSGSIRAAGNKNGALPVLAASVLASGEVRLSNV
                     PRIKDVETMVELLVDIGADAEWTGANELRIDSSGVHKADLDPELCREIRASFLLAGPL
                     LARFGRATVPPPGGDVIGRRRLDTHIHAFAELGVEVDLNGAYEMRANGLKGKRMFLDE
                     ASVMGTENAVMASVLAAGETVVGHAACEPHIQDLCRFLVSLGAHIEGIGSNILRIQGV
                     DRLEGGEYRICSDHIEVASFAALAAVAGGDVTIEDVRPDDLISIVPAFRKLGIEIDVR
                     EHTVVVPDGQELRVQDDLGDQIPKIESGIWPAFPADLTSIAVTVATQATGTILIFEKM
                     FESRLFFVDKLVSMGARIILCDPHRAVVTGPSKLYAQRLESPDIRAGMAMVIAALCAE
                     GVSTIGNIRQIDRGYEAIDERLRGLGASIERAEI"
     gene            3245..3823
                     /locus_tag="AC150V_09355"
     CDS             3245..3823
                     /locus_tag="AC150V_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3763162.1"
                     /translation="MDVTRDQAWATLTTYTKSESLQRHALAVEASVRWYARYYEEDEE
                     FWGCTALLHDFDYEIHPTLDQHPQDGAPILREEGYPEQLIEAVLSHAEHLEMPRDTQL
                     KRVLFACDELSGFVHACGLVRPTGIDGLEPKSVKKKLKQPSFAAGVHRDEVYAGAELL
                     GIELDEHIRHVVAALQPIAPELGLRTAESSSA"
     gene            complement(3834..>4179)
                     /locus_tag="AC150V_09360"
     CDS             complement(3834..>4179)
                     /locus_tag="AC150V_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763163.1"
                     /translation="GALRFEPGWIPAGALAGLLASLDLAPDWRFERAAETAARCREML
                     APLADVVPGHATLVSFRPPAGEDAASCVARLAESGVVVRELPGRELVRASCGWWTSES
                     DLERLARALAQQ"
BASE COUNT          674 a         1423 c         1436 g          646 t
ORIGIN      
        1 tgagaatcgt tcccgccatg cgtgcaccct ccgccgcgtg gctggtcggc gcggtagccg
       61 tcggcttgcc gagatcgtgc agaagtgcgg ccaggcgaac cgggagagag gcgccgatgt
      121 cggccgcacc ctgcacggtc gccaaaatgt gcccgtccag cgggaacggc tgtcgcggcg
      181 aggccagctc gtaaccgatt gcgggcgcga actccggaat cacgacgctg agcacgcctg
      241 tatcgcgcgc gatcgcgagc gcacgcgccg gctcggagcc gagcagcagt cgcgagagct
      301 cgccgtgccc atcggcggca aggccaccgc cgatccgctc gggtgcaacg tgccggattc
      361 gcgcggcgtg cagtcgcatc tgcgcgagcg tttcgccggc gaggtcgaac gcgagctgcg
      421 agacgaggcg aagcgcccgc agcattcgga gcggatcttc gctgaagctg tgcggcgtca
      481 ccgtgcgtag ctcgcggcgt ccgagcgcgg cgagaccacc gaaagggtcg acgagctcac
      541 ccgtagtcag tcgcagcgcc atcgcgttca cggtgaagtc gcggcgttcc atgtcctcca
      601 cgatcgagac cgtgccgtcc gccacgatcg agaagtcgcg gtgcccgggg ccggtgctcc
      661 gcgcggctcg agccggcgca agctcgattc ccgtcggggc gagggcggcg acgtcgcgat
      721 cgtccggctg gaaccgtacg ccgacgattt gaccgtgaac ctccatgtcg tccacccggc
      781 cgtgccgtgc gagtgcggct cgcagagccg catgatccat gtcggggacg aggaagtcct
      841 cgtccttgtg ggggatgccg agcagctcgt cgcgcacggc acccccgacg acgtaggcgt
      901 cgagttcgag cgctcggacg tattcgcgga cggcggtcgg catccgcatg attctgccgg
      961 tttcgggacg cacgtaacgg cttgtcacgt gcggacacgg cccgtcagcg gtgggctacg
     1021 gcctcgatct cgacccgccc tcccgcgggg agcgccgcga caccgatcgt cgagcgcgcc
     1081 ggcgggcgat cgccgacgtg acgcgcgtac acctcgttca tggcgtcgaa gtcggcgaga
     1141 tcgaccagga agaccgtggt cttgacgacc gcgttgatgg agctcccggc cgcttcgagg
     1201 atggcagcga tgtttcgcat gacctgctcg gtctgctcca cgacgcctgg gccaaccatc
     1261 gtcccgctcg tatcgagacc gagctgcccg gcgacgaaga cgaggtcgtc cgtcgtgatc
     1321 gcctggttgt agggagcgcc ctggaagggc gccggcgctg cttccgtccg tacgacggtc
     1381 ttctccatgc gtgctccttt cagcgttctc cggctgtggc ggcctgcgcg agcgccactc
     1441 cgagggactt gaagatcgac tcgaggacgt gatctgtgtc ttccccctcg atcagccgaa
     1501 cgtgaatagt caggcccgtc gtgtcggcga gcccgcggag gaaccgggcg gcgagatccg
     1561 tatccaggcc gcccacccgt gacgggccga gatcgacgtt cgacgccacg agcgggcgtc
     1621 ccgaggcctc gacggcgacc gtggcgagcg cctcgtcggc cggaaccgtg ccgaaaccgt
     1681 gggcgccctc cgtgagcaac ggcgcgatgg ctgtgccgag agcatctccg gccgcgtcga
     1741 cctcggcctc ggggtcgtcg ggctcgatct cgaggctcag gtcgaacttg cccgcgtggg
     1801 ccagcagacc cagcaggtgg tcgaggacgg agacgccggt ggagacgttc gagacaccag
     1861 agcccgcggc gacgcgcgca ccactcacgc cggggaatct actcctcgtg ggctgaaccg
     1921 cgaagctgcg ggtcgcctag atctctgcgc gctcgatact tgcgcccagg cctcgcaggc
     1981 gctcgtcgat cgcctcgtag ccccgatcga tctggcgaat gtttccgatc gtcgagactc
     2041 cctcggcgca gagcgccgcg atcaccatgg ccatgccggc gcgaatatcc gggctctcca
     2101 gccgctgcgc atacagcttc gagggaccgg tgaccaccgc ccgatgcggg tcgcagagaa
     2161 tgattctcgc acccatcgag acgagcttgt cgacaaagaa cagccggctc tcgaacatct
     2221 tctcgaagat cagaatcgtc cctgtagcct gcgtggcgac agtgacggcg atcgacgtga
     2281 ggtcggcggg gaacgcgggc cagattccgc tttcgatctt cgggatctgg tcgcccaggt
     2341 cgtcctggac tcggagctcc tgaccatcgg gcaccacgac cgtgtgctct ctcacgtcga
     2401 tctcgatgcc gagcttgcga aaggcgggaa cgatcgagat caggtcgtcg gggcggacgt
     2461 cttcgatcgt gacgtcgccg ccggcaacgg cggcaagagc agcgaagctt gcgacctcga
     2521 tgtgatccga gcagatccga tactcgcctc cctccagccg atcgacgccc tggatgcgaa
     2581 ggatgttcga gccgatccct tcgatgtgcg cgccgagcga gaccaagaag cggcagagat
     2641 cctgaatgtg cggctcgcac gcggcgtggc cgacgacggt ctctcccgcg gcgagcaccg
     2701 acgccatgac ggcgttctcc gtccccatca ccgaggcttc gtcgaggaac atccgcttgc
     2761 ccttgaggcc gttcgcgcgc atctcgtagg caccgttcag atcgacctcg actccgagct
     2821 cggcgaaggc gtggatgtgg gtgtcgagac gtcgtcttcc gatcacgtcg ccgcccggcg
     2881 gcggcacggt cgcccggccg aagcgggcca ggagcgggcc ggcgagcagg aacgacgcgc
     2941 ggatctcgcg gcagagctcg gggtcgaggt cggccttgtg gacgcccgac gagtcgattc
     3001 ggagctcatt cgcccccgtc cactccgcgt cggcgccgat gtccacgagc agctcgacca
     3061 tcgtctcgac gtccttgatc ctcggcacgt tggacagccg aacctcgcct gaggccagga
     3121 cggaagccgc gaggacggga agcgcgccgt tcttgttgcc ggccgcgcgg attgagccgg
     3181 aaagtggccg gccgccttcg atcacgaacg actgcatagg gcgggcggta tcgtaaccgc
     3241 ggcgatggac gtgacccgtg accaggcctg ggcgacgctc accacgtaca cgaagagcga
     3301 gtcgctccag cggcacgcac tcgccgtcga ggcctccgta cgctggtacg cgcgctacta
     3361 cgaggaggat gaggagttct ggggctgcac ggcgcttctc cacgacttcg actacgagat
     3421 ccaccccacg ctggatcagc atcctcagga cggcgcgccc atattgcgcg aggagggtta
     3481 tcccgagcag ctgatcgagg ctgtcctttc gcatgcggag cacctcgaga tgccccggga
     3541 cacgcagctg aagcgcgtcc tcttcgcgtg tgacgagctc tcgggcttcg tgcatgcgtg
     3601 cggactcgtg cgccccaccg gaatcgacgg cctcgagccg aagtcggtca agaagaagct
     3661 gaaacagccc tcttttgcgg ccggcgtgca tcgcgacgag gtctatgcgg gcgcagagct
     3721 gctcgggatc gagctcgacg agcacatccg gcacgttgtc gccgcactgc agccgatcgc
     3781 gcccgagctc ggactcagga cggccgagag ctcctcggcg taggcgatcg cgcctactgc
     3841 tgcgcgagtg cgcgcgctag ccgctcaagg tcgctttcgc tcgtccacca gccgcagctg
     3901 gcgcggacga gctctctgcc gggaagctcg cgcacgacga cgcccgactc ggccagtcgg
     3961 gcgacgcacg aggccgcgtc ctcgccggca ggcggccgga aggacaccag cgtcgcgtgg
     4021 ccgggcacga catcggcgag tggcgccagc atctcgcgac agcgcgccgc ggtctccgcg
     4081 gcccgctcga agcgccaatc cggcgcgagg tcgagcgatg ccagcaggcc ggcgagcgcg
     4141 ccggccggga tccagcccgg ctcgaagcga agcgcgccc
//
LOCUS       DBNGNK010000316         7206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1343518,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000316 DBNGNK010000000
VERSION     DBNGNK010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1343518"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="AC150V_09365"
     CDS             <1..1194
                     /locus_tag="AC150V_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3763164.1"
                     /translation="DLGLFATALAAAALFQTLLDLTVEESLTKYGFRYIAAEEWGKLR
                     RLFARALQAKLAGGLIAALVLALLAPAADALFDTDGLVVPMLVASLIPLVQASENVAT
                     SVLLLLGRYDLRGAYQSFSLALRLVGIFIGSRYGVAEAIAGIVVAQAVATVAISVVGV
                     AAFRRFPVAPQAPLADDRRGVISFVLKSSVATGLISLRTALAPLILGVVANPTQVGLL
                     RVAVAPQSGFAAASSRVRLILLTEQTRDWEHGRESHVMAGVTRYMKAALPIVGAAIPI
                     FWVTMPWLVKSVFGGEYGGAVDAARIVLLAAAIHLFLGWTKSFPTTIGRPGLRILTHG
                     IETVVLLPLVGWLGARWGVTGAAVAMLVSSLVFAFVWALLLVRIRAEVASRADRGGTP
                     GALVP"
     gene            1191..2327
                     /locus_tag="AC150V_09370"
     CDS             1191..2327
                     /locus_tag="AC150V_09370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3763165.1"
                     /translation="MKVLLVTGIWPPDVGGPASHAPALAEHLVAGGHAVEILTTAAGA
                     PEPQVYPVRWVSRALPGGVRHAAVALAVGRAAGRADVVYATSMIRRAAAGALAARRPL
                     VVKLVADEVFERSRRAGWFDGSLDEFQLEPGGLRVRALRRARLAALRQARHVFCPSAY
                     LRDTAVGWGLDAARLSVLPNPAPRLPPLPSTAAVRAELGLTGPTLAVAGRLTAQKALD
                     VALAALADVPDVALVVLGEGPERAFLEGRTAELGLGERVRFLGSGSRSDVLRLFRGCD
                     GALLCSAWENFPHTVVEALAVGTPVLATAVGGVPEVVRHDVNGLLVPPGDAAALAGTI
                     ARFFSEPGLRERLAAAAAPSVAPLEEERLLAEIAAELERAAGER"
     gene            2324..3448
                     /locus_tag="AC150V_09375"
     CDS             2324..3448
                     /locus_tag="AC150V_09375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3763166.1"
                     /translation="MKRRLLMVSRTRYALPLSTTLARKFDALSAQLDVRVLAARADGS
                     QGGDRRFQLFRPIRPRRLDGLAFYALLPYRVARELRRSDPDVVLVQGAQEAALALLGR
                     AVARVPAKVVVDVHGDPGAATRLYGSRARGLLSGLGDGMARLALRRADGVRTISGYTS
                     RLVRDEGVEPSATFPAFMDLEPFLVSAPQPLPVQPAALFVGVLERYKAVDVLADAWRR
                     AAPRVPGAVLHVVGRGTLTDVVEGLVADLPDRTRWSPVLSTAEVAHALDQATVLLLPS
                     RSEGLGRVVIEAFCRRRGVIGSSVGGIPDLIEDGVNGLLVPTEDAEALADAIVRVLSD
                     SELAERIGQAGHASVRAWTATPEEYAFRVRELVEAVAGRR"
     gene            3445..4464
                     /locus_tag="AC150V_09380"
     CDS             3445..4464
                     /locus_tag="AC150V_09380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3763167.1"
                     /translation="MRLVFVTQQVDPSSPVLGATVAKIRALAAGVDEVVVLADGAVDG
                     VLPENCQIRRFGARTKAGRGARFEAALAWELGRRPRPAAVIAHMCPIYAVLAAPLARP
                     LGVRILLWFTHWKTSHLLVAAERVSDSVITVERRSFPLASAKVVPIGHGIDLGRYSCD
                     EPPARQGLSLLALGRTSPAKGLDTVLRGVAMVPEARLLVRGPSLTDEERECRASLERL
                     ASELGVDDRFELGGPVPYEQVPALLATADGLVNNMRAGATDKVVYEAAASCRPVLASN
                     PALDTFLPAQLRFPRDDPGALADRVRELARADRRALGRELRDRVEREHSAEGWARRVV
                     EVARA"
     gene            4461..5564
                     /locus_tag="AC150V_09385"
     CDS             4461..5564
                     /locus_tag="AC150V_09385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3763168.1"
                     /translation="MTARVVLHAAKVSGISGAENHLLAVLPRLREAGFDVRFAMLHEG
                     EQGARDFADRLEAGGVPVADVRISRDLSPGAFRGLLALARRLEPALLHTHLVHADLYG
                     LAAGAVARVPVRVSTKHGFNPFRERPGFGTVDRAAARLAHVHIAISHGLAHYLASTEG
                     FSESAFEVVHYGIDAGPPPPPPPKRPSIACVGRLIPIKGHETLLRAVALARGKVPGLE
                     LEIAGGGPLESELRALVDALALADTVRFLGSVTPPQPVFERAAIVAVPSLGEGFGMVA
                     LEAMERGRAVIASDVCGLPEIDADGESGVLVPPGDAAALAAAIVALAAGPSRVAEMGA
                     SGRARALRDFTQERCVGRLVELYLGALSRAGVE"
     gene            complement(5528..6814)
                     /locus_tag="AC150V_09390"
     CDS             complement(5528..6814)
                     /locus_tag="AC150V_09390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR3763169.1"
                     /translation="MRRVHAYLLVALLAAVPRLAVLAVERGEILEAFVEKSDRFAQTF
                     VDSGTFGFLPDVPSAYTQPLYSFFLSALYWPFGRSWLVVGLAQTAIAVATALLVLELG
                     RRLRSLAVGVVAASIATLHPYVVWHDVHVNREILDGLIVVALALLALVAYERRSLPLA
                     AGAGAMCGLAILGNSRLLLLPFALAPFVAWRVRPGSRALAAGVVVVLAAALVVAPWAV
                     RNRVQVGCLAITTDARALWKANNPQTYDVLAEGRWIDDVAELPGAPPWPELAADLTLG
                     GMPTAVDECEQVVLYRDEVLAFWRDEPGEKARLAAQATRMLWSPTFTVEVDESSRNAA
                     VEFAKKTFEPAFMIALYALAIWGLFLAPSRYVALVLLLAAYNTLMAMVFAGTVRYRAP
                     FDALLAVLAAFALERAWTLVRRRRGYSTPARESAPR"
     gene            6843..>7206
                     /locus_tag="AC150V_09395"
     CDS             6843..>7206
                     /locus_tag="AC150V_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763170.1"
                     /translation="MGTTKRYAVVSCHVELLGDDRVWTRYRAFLESGHGFRIASLVRP
                     PDSAAGESEQQWLERARVLARLGPFGQHTHWTSPTHARPTGGGTGARVLREGAWLREH
                     GLEPTLFVGGGWYTDESVA"
BASE COUNT          912 a         2505 c         2656 g         1133 t
ORIGIN      
        1 gacttgggcc tctttgcgac ggcgctcgcg gccgccgctc tgttccagac gctgctcgac
       61 ctcacggtgg aggagtcgct gaccaagtac ggcttccgct acatcgccgc ggaggagtgg
      121 gggaagctga ggaggctgtt cgcgcgcgcg ctgcaggcga agctcgcggg cggactgatc
      181 gcggcgctcg tgctcgcgct tctcgcgccc gcggccgacg ccctgttcga caccgacggt
      241 ctggtcgtgc cgatgctcgt cgcctccctg atcccgctcg tgcaggcgtc cgagaacgtc
      301 gccacgtcgg tgctcctctt gctcgggcgc tacgacctgc gcggcgcata ccagagcttc
      361 tcgctcgccc tgcgactggt cggcatcttc atcgggagcc gctacggcgt cgcggaggca
      421 atcgcgggaa tcgtggtcgc acaggcggtc gccaccgtgg cgatcagcgt ggtgggcgtc
      481 gcggcgttcc gccggtttcc ggtcgcaccg caggcgccgc tcgcggacga ccgccgcggc
      541 gtgatctcct tcgtgctcaa gtcgagtgtc gccaccggtc tcatctcgct ccgaactgcg
      601 ctcgcgccgt tgatcctcgg tgtcgtcgcc aaccccacgc aggtggggct gctgcgcgtg
      661 gcggtggcgc cgcagagcgg cttcgccgcg gcctcgtccc gggtgcgcct gattctgttg
      721 acggaacaga cgcgtgactg ggagcacggg cgcgagagcc acgtgatggc gggcgtgacg
      781 cggtacatga aggcggcgct cccgatcgtc ggtgccgcga tcccgatctt ctgggtcacg
      841 atgccctggc tggtcaagag cgtcttcggg ggcgagtacg gcggtgccgt cgacgctgcc
      901 cggatcgtgc tgctcgccgc tgcgattcac ctgttcctcg gctggacgaa atcgttcccg
      961 accacgatcg gacggcccgg gttgcgcatt ctcacgcacg gcatcgagac agtggtgctg
     1021 ctaccgcttg tcggctggct cggcgcccgc tggggcgtga ccggcgccgc cgtcgcgatg
     1081 ctcgtctcga gtctcgtttt cgcgttcgtg tgggcgctgc tccttgtccg gattcgagcc
     1141 gaggtcgcca gccgcgccga tcgcggtggg actcccggtg cgctcgtccc gtgaaggtgc
     1201 tcctggtcac cgggatctgg ccgcccgacg tcggtggccc ggcaagccat gcgccggcat
     1261 tggcagagca cctcgtggca ggcgggcacg ccgtcgagat cctgacgacg gccgctgggg
     1321 cgccggagcc gcaggtgtat ccggttcggt gggtgtctcg ggccctgccg ggcggcgttc
     1381 gccacgccgc ggtggcgctg gcggtcggta gggccgccgg ccgcgccgac gtcgtctatg
     1441 cgacgagcat gatcaggcgg gccgcggctg gggccttggc tgcacggcgg cctcttgtcg
     1501 tcaagctggt cgccgacgag gtgttcgagc gctcgcggcg cgccggctgg ttcgacggca
     1561 gcctggacga gttccagctc gagcccggag gcctgcgcgt gcgggcactc cgccgcgcgc
     1621 gactcgccgc gctgcggcaa gcgcggcacg tcttctgccc gagtgcgtat ctccgcgaca
     1681 ccgccgtcgg ctggggactg gacgccgccc gcctgtccgt cctccccaac cccgccccgc
     1741 ggctgccgcc gcttccttcg accgccgccg tacgcgccga gctcggactc accggcccca
     1801 cgctcgccgt cgcaggcagg ttgaccgccc agaaggcgct cgacgtcgct cttgccgcgc
     1861 tcgcggacgt tccggacgtc gcgctcgtgg tgctcgggga gggaccggaa cgggctttcc
     1921 tcgaggggcg cacagccgag ctcggcctgg gcgagagggt tcgatttctc ggcagcggta
     1981 gccgctcgga cgtcctccga ctgttccgtg gctgcgatgg ggcgctgctg tgctccgcgt
     2041 gggagaactt cccgcacacg gtggtcgagg ccctggccgt cggcacgccg gttctcgcga
     2101 ccgcggttgg cggcgtgcca gaggtcgtgc gccacgacgt gaacggcctg ctggttccgc
     2161 ccggcgacgc cgctgccctg gccggcacga tcgcccgctt cttttccgag cccgggctcc
     2221 gtgagcgact cgcggctgct gccgccccct cggtcgctcc cctggaggag gagcgcctgc
     2281 tggccgagat cgcggcggag ctcgagcgag ctgcgggtga gcggtgaagc gacgcctcct
     2341 gatggtcagc aggactcggt atgcgctccc gctctcgacg acgctcgcga ggaagttcga
     2401 tgctctgtcg gcccagctcg acgtgcgggt gctggcggcg agggcggacg gcagccaggg
     2461 cggggaccgc aggttccagc tgtttcgacc gatccggccg cggcggctgg acgggcttgc
     2521 cttctacgcg ctgttgccct atcgcgtcgc tcgcgagcta cggcgctccg accccgacgt
     2581 cgttctcgtc caaggcgctc aggaggcggc tttggcgctg ctcgggcggg ccgtcgcccg
     2641 cgtcccggcg aaagtcgtcg tcgacgtcca cggcgacccg ggcgccgcca ctcgcctgta
     2701 cggctcgcgg gcgcgtgggc tgctctcggg gctcggcgac ggcatggcgc gtctggccct
     2761 gcgacgggca gacggcgttc gcacgatctc cgggtatacg agccggctcg tgcgcgacga
     2821 gggggtcgag ccgtcggcga cgtttcccgc cttcatggat ctcgagccgt tcctcgtcag
     2881 cgccccgcag ccccttcctg tgcagccggc cgccttgttc gtcggcgtcc tcgagcgcta
     2941 caaggcggtc gacgtgctcg ccgacgcgtg gcggcgcgca gccccgcggg tgccgggcgc
     3001 ggtgctccac gtcgtcggtc gcgggacgct gacggacgtc gtcgaaggac tggtcgccga
     3061 tctgcccgac cgaacgcgct ggtcacctgt gctctcgacg gccgaggtcg cgcacgcact
     3121 cgatcaggcg acggtgctgc tccttccgtc ccgctcggaa gggctcggcc gcgtggtgat
     3181 cgaggccttc tgtcgtcgtc ggggcgtgat cggctcgagc gtcggcggca tccctgacct
     3241 gatcgaggac ggcgtcaacg ggctgctcgt gcccacggag gacgccgagg cgctcgcaga
     3301 cgcgatcgtg cgagtcctct ccgactccga gctggcagag cggatcgggc aggcggggca
     3361 cgcgtccgta cgggcgtgga cggcgacgcc cgaggagtac gcattccggg tccgcgagct
     3421 ggtcgaggcg gtggccgggc gccggtgagg ctcgtcttcg tcacgcagca ggtcgacccg
     3481 tcgagcccgg tgctgggagc cacggtggcg aagatccgcg cgctcgcggc cggcgtcgac
     3541 gaggtagtcg tcctggccga cggcgcggtc gacggcgtgc tccccgagaa ctgccagatc
     3601 cgtcgcttcg gtgcgcgcac gaaggccgga cgtggcgcac gcttcgaggc tgcgctcgcc
     3661 tgggagctgg gtcggcgtcc ccgaccggcg gcggtgatcg cccacatgtg cccgatctat
     3721 gcggtgcttg cggctccttt ggctcgacct ctcggcgttc ggattctgct ctggttcacg
     3781 cactggaaga cgagccacct cctggtcgcg gccgagcggg tctcggactc cgtcatcacg
     3841 gtcgagaggc gttcgttccc gcttgcctcg gcaaaggtcg taccgatcgg gcacggcatc
     3901 gacctgggtc gctactcgtg cgacgagccg cccgcccgcc aaggcctgtc gctcctggcg
     3961 ctcgggcgta cgtcgccggc gaagggcctc gacacggtgc tgcgcggggt cgcaatggtt
     4021 cccgaagccc ggctgctggt cagagggccg tcgctcacgg atgaggagcg ggaatgccga
     4081 gcatccctcg agcggctggc ctcggagctt ggcgtcgacg accggttcga gctcggcggt
     4141 cccgtcccct acgagcaggt gccggcactg ctcgccacgg ctgatgggct cgtgaacaac
     4201 atgcgggccg gcgcgacgga caaggtggtc tacgaggctg cggcgtcctg ccggcccgtg
     4261 ctcgcctcga atccggctct cgacacgttc ctccccgctc agcttcgctt cccccgagac
     4321 gacccgggag cgctggccga tcgggtgcgg gaactcgccc gggccgatcg acgcgcactc
     4381 gggcgcgagc tgcgcgaccg ggtcgagcgc gagcactccg cggagggatg ggctcgacgt
     4441 gtcgtcgagg tggcaagggc atgacggcgc gggtcgtgct ccacgccgcc aaggtctccg
     4501 gcatctcggg ggccgagaat catctcctgg ccgtcctgcc ccggctgcgg gaggcgggtt
     4561 tcgacgtccg cttcgcgatg ctccacgagg gcgagcaggg ggcgcgcgac ttcgccgaca
     4621 ggctcgaggc aggtggcgtc ccggtggccg acgtccggat ctcgcgcgac ctctcgcccg
     4681 gtgcgtttcg gggcctcctc gctcttgcca ggcgcctcga gcctgcgctc ctccacacgc
     4741 acctcgtaca cgccgatctc tacggcctcg cagcaggggc cgtcgcgcgc gtgccggtgc
     4801 gcgtgagcac gaagcacggc ttcaatccct ttcgggagcg ccccggcttc ggcacggtcg
     4861 accgggccgc ggcgcggctc gcccacgtcc acattgcgat ctcgcacggg ctcgcccact
     4921 acctcgcgtc gaccgaagga ttctccgaga gcgcattcga ggtcgtccac tacggcatcg
     4981 acgcaggccc gccgccgccg ccgccgccga agcggccttc gatcgcttgc gtcggccgcc
     5041 tgatcccgat caagggtcac gagaccctgc tccgggcggt cgcgcttgcg cgtgggaagg
     5101 ttcccgggct cgagctggag atcgcaggcg gcggaccgct cgagtcggag ctccgcgctc
     5161 tcgtggacgc gctcgctttg gcggacaccg tccgattcct cggcagcgtg accccgccgc
     5221 aaccggtctt cgagcgcgcg gcgatcgtgg cggttccctc actcggcgag gggttcggca
     5281 tggtcgccct cgaggcgatg gagcgcggcc gagccgtcat tgcgagcgac gtgtgcgggc
     5341 tgcccgagat cgacgccgac ggcgagtcgg gagtccttgt gccgccgggc gacgcagcgg
     5401 ctcttgccgc cgcgatcgtc gcgctggcag cgggcccgtc gagggtggcc gagatgggcg
     5461 cgtccggacg cgctcgcgcg cttcgcgact tcacgcagga gcgctgcgtc gggcggctcg
     5521 tcgagctcta cctgggagcg ctctcccgag cgggcgtcga gtagccgcgc cggcgacgga
     5581 cgagcgtcca ggcgcgctcg agcgcgaagg cggccaggac ggcgagcagc gcgtcgaaag
     5641 gcgcccggta gcgaaccgtt ccggcgaaca ccatcgccat gagcgtgttg taggcggcga
     5701 ggagcagaac gagcgcgacg tagcggctcg gtgcgaggaa gagtccccag atcgcgagcg
     5761 cgtagagcgc gatcatgaac gcgggctcga aagtcttctt cgcgaactcg accgcggcgt
     5821 tccgcgacga ctcgtcgacc tcgaccgtga aggtcggcga ccagagcatg cgcgtcgcct
     5881 gagcggcgag gcgcgccttc tcgccgggct cgtcccgcca gaacgcgagc acctcgtccc
     5941 tgtagaggac gacctgctcg cattcgtcga ccgccgttgg cattccgcca agtgtcagat
     6001 cggcggcgag ctccggccag ggcggtgcgc cgggaagctc agccacgtcg tcgatccacc
     6061 tgccctcggc caggacgtcg tacgtctgcg ggttgttggc cttccagagg gctcgtgcgt
     6121 cggtcgtgat ggcgaggcag ccgacctgca cccggttgcg gacggcccac ggggccacga
     6181 cgagtgccgc ggcgagcact acgacgacac ccgccgcgag ggcccgcgac ccaggacgaa
     6241 cccgccaggc cacgaacggc gccagggcga acgggagcag caggagtcgg gagttgccga
     6301 ggatcgcgag gccgcacatc gctccggcgc ccgcggcgag cggcagggat ctgcgctcgt
     6361 aggcgacgag cgccagcagc gcgagcgcca cgacgatgag accgtcgagg atttcgcggt
     6421 tgacgtggac gtcgtgccac acgacgtagg ggtggagcgt cgcgatggaa gcggcgacga
     6481 cgccgacggc gagcgagcgg agccggcggc cgagctccag gacgaggagc gccgtggcga
     6541 cggcgatcgc ggtctgggcg agcccaacga cgagccacga ccgcccgaac ggccagtaga
     6601 gcgccgagag gaagaacgaa tacagcggct gtgtgtaggc cgatgggacg tcgggcagga
     6661 agccgaaggt gccgctgtcg acgaacgttt gcgcgaagcg gtcgcttttc tcgacgaatg
     6721 cctcgaggat ctccccgcgt tccaccgcga gcacggcgag tcgagggacg gcggcaagca
     6781 gcgctacgag cagatacgcg tgaacacggc gcacggacgg cctccgtgat atgcgtctgc
     6841 ctgtgggcac cacgaaacgg tacgcggtcg tctcctgcca cgtcgagctg ctcggagacg
     6901 atcgtgtctg gactcgctac cgggctttcc tcgagtcggg ccacgggttc cggatcgcgt
     6961 cgctcgtccg tccgccggat agcgcggctg gggagtcgga gcagcagtgg ctcgagcggg
     7021 cgcgcgttct ggcccgcctc ggccctttcg gccagcacac acattggacg agcccgacac
     7081 atgcgcgtcc cacgggcggg ggcacgggcg cacgcgttct gagagagggg gcgtggttgc
     7141 gcgagcacgg cctcgagccg acgctcttcg tcggcggcgg ctggtacacg gacgagtccg
     7201 tcgcgg
//
LOCUS       DBNGNK010000317         4678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1700569,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000317 DBNGNK010000000
VERSION     DBNGNK010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1700569"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /gene="pheT"
                     /locus_tag="AC150V_09400"
     CDS             complement(<1..1286)
                     /gene="pheT"
                     /locus_tag="AC150V_09400"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR3763171.1"
                     /translation="MRVPLTWLREYVETDASAEQIARRLAISALEVDRVIDMGVEDAA
                     GNLDAFLVGKVLEAGKHPNADKLQLCRVDVGEGEPRQIVCGAWNFGAGATVAVALPGA
                     RLPGADEPLAEVKLRGELSRGMILSERELKLGTDHSGVIVFDAGPEPGTPLVDALPIR
                     EQVLEVTPTLNRVDLLSVYGIAREVSALLGGELRPMPGEDPPILHPEWVDVRIDDAEG
                     CPRYIGRVFRKVLVGPSPLWLRARMHFAGMRAISNIVDITNYVMHALGSPIHAFDREK
                     LHDGRIVVRRAERGEEVRTLDGQLRRLDERDLLIADADRAVALAGIMGGLETEVSDGT
                     SEVLLEAANFEAISLLKTSERLGLRTDGSNRWEKGVDPHLAEPAAVFASQLIVELTGA
                     ELTGSADVHGDLPARPVVRLRPERADRVIGLSVPAE"
     gene            complement(1286..2281)
                     /gene="pheS"
                     /locus_tag="AC150V_09405"
     CDS             complement(1286..2281)
                     /gene="pheS"
                     /locus_tag="AC150V_09405"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006363086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR3763172.1"
                     /translation="MDARALEQEALAAVAAASSVDEIEAVRVEYLGRKSALKRALREV
                     RDRETGIALNAVRETLESAVADREQSLLRAELDHKLNEERVDVTLPGRPYPRGRLHLI
                     SQIRREVEDIFLGLGYTVVDGREVETTRYNFDGLNFPGGHPARSPLSTLFLDDETVLR
                     TETSPSQIRTMEAQDPPIYMVSLGRVYRRDTPDATHTPTFHQIEGLAVDEGITLGDLM
                     GTLDYLFKALFGEERRTEFRTHYFPFTEPSIEAYVSCHVCEGSGCTVCRHSGWIEVGG
                     AGVVDPALFEFVGYDPERYTGFAFGWGLERIAVLRHGIPDLRELWRNDLRLLRQF"
     gene            2402..3133
                     /locus_tag="AC150V_09410"
     CDS             2402..3133
                     /locus_tag="AC150V_09410"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763173.1"
                     /translation="MALYDAIAGIYDPWSKSVTEDIGFYVEEAVAAGGPVVELAVGTG
                     RIAVPTAKAGISVVGVDQSEAMLAVARAYAEREGVGEQIDLRVGDMRKPPVSERAPLV
                     TIPFRSLLHLADDRERICALRAARDLLEPGGRVIFDVFTPSSEDIAETNGRWLEREPG
                     IFERADWDEGARKLSLSVRGNDEEARMELYWLSAQEWRRLIDAAGMDVLHLYGWFDRR
                     PFDGGEDSIWIVGARDETRATSSPS"
     gene            complement(3114..>4678)
                     /locus_tag="AC150V_09415"
     CDS             complement(3114..>4678)
                     /locus_tag="AC150V_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HIR3763174.1"
                     /translation="VDPDDTSLLPQIIGLPGVGFFLAASSGSWSAFPSENLEYAFEWL
                     RCSGSSVGSCVAIAGANEDVYKATSSDAGSRIRVRVTASNGVAPPGIAVSPATGPVAA
                     AGGGGGVGGGAGADLIVLISASTSGGKVAFNVTARNLGNVRADGVTLTGGFAPGLVPT
                     SATTNRGSCASATSCAIGSLAPGESALVSMGAQAATTGSYKFTATVQSATPDVNPSNN
                     SLTVSAFVTASSGPPKATDAKVSGASGSASANAALKKVPPTLKAMKVKRTWVVDTKFS
                     LLSGAAKLQLTVTEDRDLEQLRLLKGSRLGPVRVKKNLVNKKPVTTLTFNAKRKGSFP
                     LRIVLPAKGFALAKSYVIRITATANGQSSTLDLGFRGSKLVQKKTPLSYGGKNLVAAS
                     KFFVPKDKAKVGVKSWVTKGKSNAQLRVLKGSRLGKTITKSARRQLGFSAAKRGSYPV
                     KMILPVTGFAGKLWYTMHVDSRPKNAAWTTYEIRFRASKALLNKLSRAVASAKKKTGS
                     KAGAKTVKKAKK"
BASE COUNT          716 a         1562 c         1617 g          783 t
ORIGIN      
        1 ttctctgcag gaacgctcag tccgatcacc cgatcggctc gctcgggccg gagccgtacg
       61 accggacgcg ccgggaggtc gccgtggacg tcggccgagc ccgtcagctc cgcccccgtc
      121 agctcgacga tcagctggct cgcgaagacg gccgccggct cggccagatg cgggtcgaca
      181 cccttctccc agcggttcga gccgtcggtg cgcagaccga ggcgctccga ggtcttgaga
      241 agggagatgg cctcgaagtt cgcggcttcg agcagaacct cgctggtccc gtcggagact
      301 tccgtctcga gtccgcccat gatcccggcc agcgcgaccg cgcggtcggc gtctgcgatc
      361 agcaggtcac gctcgtcgag tcggcgcaac tggccgtcga gcgttctgac ctcctcccca
      421 cgctcggcgc ggcgcacgac gatacggccg tcgtgaagct tctcgcgatc gaaggcgtgg
      481 atcggactgc cgagcgcgtg catgacgtag ttggtgatgt cgacgatgtt ggagatcgcg
      541 cgcatcccag cgaagtgcat gcgggcgcgg agccagagcg gcgagggccc gaccagaacc
      601 ttgcggaaga cccggccgat gtagcgcggg cacccctcgg cgtcgtcgat gcggacgtcg
      661 acccattcgg gatggagaat cggcggatct tcgccgggca tcggacgaag ctcgccgccg
      721 agcagcgccg acacctcgcg cgcgatcccg tagaccgaga gcagatcgac gcgattcaac
      781 gtcggcgtca cctcgagcac ctgctcccgg atggggagtg cgtcgacgag aggcgtcccg
      841 ggctcggggc cggcgtcgaa cacgatgact cccgagtggt cggtgccgag cttgagctcg
      901 cgctccgaga ggatcatccc gcgcgagagc tctccccgga gcttcacttc ggcgagcggc
      961 tcgtctgctc cgggcagcct ggccccgggc agcgcgaccg caacggtcgc gcccgcgccg
     1021 aagttccagg caccgcagac gatctgccgc ggctcgccct cgcccacgtc gacccggcag
     1081 agctgcagct tgtccgcgtt cggatgcttc cccgcctcga gcaccttgcc gaccaggaaa
     1141 gcgtcgaggt tgcccgcggc gtcctcgacg cccatgtcga tcacacggtc gacctcgagg
     1201 gcggagatcg cgagccggcg cgcgatctgc tcggcgctcg cgtcggtctc gacgtactcg
     1261 cgcagccatg tgagcggcac cctcatcaga attgcctcag cagtcgcagg tcgttgcgcc
     1321 agagctcacg cagatccggg atgccgtgcc ggagcaccgc gatgcgttcc aggccccagc
     1381 cgaacgcgaa gccggtgtat cgctcggggt cgtagccgac gaactcgaag agcgccggat
     1441 cgacgacgcc ggcgccgccg acctcgatcc agccggagtg ccggcagacc gtgcagccac
     1501 ttccctcgca gacgtgacag gagacgtacg cctcgatcga gggctcggtg aagggaaagt
     1561 aatgcgtgcg gaactcggtg cgccgctcct cgccgaagag cgccttgaag aggtagtcga
     1621 gcgtgcccat cagatccccc agcgtgatgc cctcgtcgac cgcaagaccc tcgatctggt
     1681 ggaacgtcgg cgtgtgggtg gcgtcgggcg tgtcgcggcg gtagacgcgt ccgagcgaca
     1741 ccatgtagat gggcgggtcc tgcgcttcca tcgtgcggat ctgcgacggc gaggtctccg
     1801 tccggagcac ggtctcgtcg tcgaggaaga gggtcgagag cggcgagcga gccgggtggc
     1861 cgcctggaaa gttgagcccg tcgaagttgt accgggtcgt ctcgacctcg cggccgtcga
     1921 cgaccgtgta gccgagcccg aggaagatgt cctcgacttc gcggcggatc tggctgatca
     1981 ggtggagacg gccgcgcggg tagggccggc cgggcagggt cacgtccaca cgctcttcgt
     2041 tcagcttgtg gtcgagctcc gcgcgaagca gcgactgctc ccggtcggct accgcgctct
     2101 cgagggtctc gcggaccgcg ttcagagcga tgcccgtctc acggtcgcgc acctcgcgga
     2161 gtgcccgctt gagcgcgctt tttcgtccga ggtactcgac ccggacggct tcgatctcgt
     2221 cgacggacga cgcggcggca acggcggcga gtgcctcctg ctcgagggcc cgggcgtcca
     2281 tcgggacagc gagcctagca accgctccgg cgcaagtaac agactgttac ttatgggcct
     2341 tcaacgtcga ccgagagccg aaggccgccg gtagcagtgt cttgcttgct gtaatcggca
     2401 ggtggccctg tacgacgcga tcgccggcat ctacgacccg tggagcaaga gcgtgaccga
     2461 ggacatcggc ttctacgtcg aggaggccgt cgccgccggc ggcccggtcg tcgagcttgc
     2521 ggtcggcacc ggcaggatcg ccgtgccgac ggcgaaggcg ggcatcagtg tcgtcggtgt
     2581 cgaccagtcc gaggcaatgc tcgccgtcgc ccgtgcctac gccgagcggg aaggcgtggg
     2641 cgagcaaatc gacctgaggg tgggcgacat gcgcaaaccg ccggtgtccg agcgcgctcc
     2701 gctcgtgacg atccccttcc gctccctgct ccacctcgcc gacgaccggg agcggatttg
     2761 cgccctccgc gccgcgaggg acctgctgga acccggcggc cgggtcatct tcgacgtctt
     2821 cacgccgagc tcggaggaca ttgccgagac aaacggccgt tggcttgagc gcgagccggg
     2881 catcttcgag cgcgcggact gggacgaggg cgcgcggaag ctgagcctgt cggtgcgcgg
     2941 gaacgacgag gaggcccgca tggagctcta ctggctgtcg gcgcaggagt ggcggcggct
     3001 gatcgatgcc gccgggatgg acgtgctgca cctgtacggc tggttcgacc ggcgcccgtt
     3061 cgacggcggc gaggactcga tctggatcgt cggcgcccgg gacgagacgc gggctacttc
     3121 ttcgccttct tgacggtctt ggcgccggcc ttcgagccgg tcttcttctt cgcggatgcc
     3181 accgctcgac tgagcttgtt cagcagcgcc ttcgatgccc tgaagcggat ctcgtaggtt
     3241 gtccaggcgg cgttcttcgg gcgggagtcg acgtgcatcg tgtaccagag cttgccggcg
     3301 aagcccgtca ccgggaggat catcttcacc gggtagctgc cgcgtttggc cgcgctgaag
     3361 ccgagctggc gacgagcaga cttcgtgatg gtcttgccga gccggcttcc cttgagcact
     3421 cgcagttggg cattgctctt gcccttcgtg acccatgact tcacaccgac cttggccttg
     3481 tccttgggca cgaagaactt ggacgcggcg acgaggttct tgcctccgta actgaggggc
     3541 gtcttcttct gcaccagctt gctgcctcgg aaaccgaggt cgagcgtcga gctctggccg
     3601 ttggcggtcg cggtgatgcg gatcacgtag ctcttggcga gcgcgaagcc tttggccggg
     3661 aggacgatcc gcaacgggaa actgcctttc ctcttcgcgt tgaacgtgag cgtcgtcacg
     3721 ggcttcttgt tcaccaggtt cttcttcacc ctgacgggcc cgagccggct gcccttgaga
     3781 agacggagct gctcgaggtc gcgatcctct gtcaccgtca gctgcagttt ggcggcaccc
     3841 gagagaagcg agaactttgt gtcgacgacc cacgtcctct tgaccttcat cgccttgagc
     3901 gtcggaggca ccttcttgag agccgcgttg gcgctggcgg atccgcttgc gcccgagacc
     3961 ttcgcatcgg tcgccttcgg cggaccgctg ctcgcggtca cgaatgccga gacggtcagg
     4021 ctgttgttcg acgggttcac gtccggggtt gcggactgca ccgtcgccgt gaacttgtac
     4081 gagcctgtgg tcgcagcctg ggctcccatc gacacgagcg ccgactcgcc gggagcgagc
     4141 gagccaatcg cgcaggacgt ggcgctcgca cacgagccgc ggttggtcgt ggccgacgtg
     4201 ggaacgagcc cgggagcgaa tcctcccgtg agcgtgacgc cgtcggcccg tacgttgccg
     4261 aggttcctcg ccgtcacgtt gaacgcgacc ttgccgcccg acgtcgacgc gctgatcagc
     4321 acgatcagat ccgctcccgc ccctcctccg acgccaccgc cgccgcctgc ggcggccact
     4381 gggccggtcg cgggtgagac tgcgatgccg ggaggcgcga cgccgttcga cgcggtcacg
     4441 cgcacccgga tgcgtgagcc ggcgtcggac gaggtggctt tgtagacatc ctcgttcgct
     4501 cccgcgatcg ccacacagga gccgacggag ctgcccgagc agcgcagcca ttcgaacgcg
     4561 tattcgaggt tctcgctcgg gaacgccgac caggatccac tcgaggcagc gaggaagaag
     4621 ccgactcccg gcaggccgat gatctgcggc agcagcgacg tgtcgtccgg atcgacga
//
LOCUS       DBNGNK010000318         3859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1919017,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000318 DBNGNK010000000
VERSION     DBNGNK010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1919017"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1444
                     /locus_tag="AC150V_09420"
     CDS             <1..1444
                     /locus_tag="AC150V_09420"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763175.1"
                     /translation="LAFFTRTRTGEVQSRIAGDIGGIETVVTSTATSVLSNVTTVLAT
                     IVAMVLLDWRLAVFALALLPLFVWLTKRVGDRRRKVTGERQASLADVSAIVQESLSVS
                     GILLGKTMGRSEDLAQRFEKESSRLAELEVRSRMTGRWMMAAIQMTFAIMPALVYWFA
                     GFTIAQGNAAITLGTLVAFTTLQARLFFPIGGLLGVQLEVHSSLALFDRIFDYLDQPV
                     DIAPGARSLAGARGEVAFDDVWFRYGGDAWTLEDVSFSIPAGTKTALVGETGSGKTTC
                     AYLVARLYDATEGAVTIDGVDVRELTFESLAATVGVVSQETYLFHASVRENLRFARPE
                     ATDAEIEEAARAAQVHELIATLSEGYDTVVGERGYRFSGGEKQRIAIARTILRNPPIL
                     VLDEATSALDTQTERLVQEALERLAEGRTTIAIAHRLSTVRDADQIVVLDRGRIAEIG
                     THDDLLAAGGRYAGLVRRDADTAIERQPIG"
     gene            1565..3136
                     /gene="pckA"
                     /locus_tag="AC150V_09425"
     CDS             1565..3136
                     /gene="pckA"
                     /locus_tag="AC150V_09425"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR3763176.1"
                     /translation="MVSLDEHGVQAGGRVIRNPTTPMLYEHALARGDGRLAAGGPLAV
                     DTGVHTGRSPKDKFVVREPQSESRIWWGAVNKPIAEDKFEGLRAKVIEHLAGQDPLYV
                     VDAFAGADPAHRICVRILTGSPYHALFSKTMFIDPSDQELASFEPTAVVFHAPEVEAD
                     PDTDGTRSSTFVCLHPTRDEVVIGGTFYAGEIKKSIFTVMNDRMPLEGVFPMHCSANV
                     GKDGRVAVFFGLSGTGKTTLSADPERSLIGDDEHGWGDNGVFNFEGGCYAKVIRLSAE
                     AEPDIFRTTHTFGTVLENVVLDERGVLDLDDDSKTENTRAAYELEQIGNSLPTKMAGH
                     PSAVVMLTADAFGILPPVARLSKDQAMYWFLSGFTAKLAGTEIGVTEPQPTFSSCFGA
                     PFLPQPPAVYAGMLGEKLDRHGASVWLVNTGWTGGAYGDGHRMPIDATRALLHAALGG
                     SLDGADFRIDPVFGFEVPVAVHGVDDALLDPRSTWKDSDAYDRKAQELAGMFRDNFAK
                     LEGVDPSLAAAGPAG"
     gene            3194..>3859
                     /locus_tag="AC150V_09430"
     CDS             3194..>3859
                     /locus_tag="AC150V_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR3763177.1"
                     /translation="MEVTHDVLVVGAGCAGMRAAIEAHDRGANVGVISKLHPTRSHSG
                     AAEGGINAALGNTAMDSPETHAFDTVKGSDYLGDQDAIEVFAAEAPGDIYQLEHWGAV
                     FSRNEEGKLDQRPFGAAGSPRTVFSADITGHVLIHVLYEQLMKRMAEGTKVYEEFFAW
                     RLVVEDGRCTGVICWDLVNGGLHYLTGKAVILATGGAGRLYRATTNAYACTGDGMAMA
                     LRAG"
BASE COUNT          637 a         1307 c         1307 g          608 t
ORIGIN      
        1 gctcgcgttc ttcacgcgaa cccgcacggg cgaggtgcag agccggatcg cgggcgatat
       61 cggcggcatc gagaccgtcg tcacgtcgac cgcgacgtcg gtgctgtcca acgtgaccac
      121 cgttctcgcg acgatcgtcg ccatggtgct gctcgactgg cgtctcgcag tcttcgcgct
      181 ggcgctgctg cccctcttcg tctggctgac gaagagggtc ggtgaccggc gccgcaaggt
      241 caccggcgag cgtcaggcct cgctggcaga cgtgtcggcg atcgtgcagg agtccctgtc
      301 cgtctccggg atcctgctgg gcaagaccat ggggcgaagc gaggatctcg cccagcgctt
      361 cgagaaggag tcgtcgcggc tcgcagagct cgaggtgcgg agccgcatga cgggccggtg
      421 gatgatggcc gcgatccaga tgaccttcgc gatcatgccc gcgctcgtct actggttcgc
      481 cggcttcacg atcgcgcaag ggaacgcggc catcacgctg ggcacgctgg tcgcgttcac
      541 aacactgcag gcacgcctct tcttcccgat cggcggtctt ctcggcgtcc agctcgaggt
      601 tcacagctcg ctcgccctct tcgaccggat cttcgactat ctcgaccagc cggtggacat
      661 cgcaccgggg gcgaggagcc tcgcgggcgc acgcggcgag gtcgcgttcg acgacgtgtg
      721 gttccgctac ggcggcgacg catggacact cgaggacgtg tcgttttcga ttccagcggg
      781 aacgaagacg gccctcgtcg gcgagaccgg ttccggtaag acgacctgcg cctacctcgt
      841 cgctcggctc tacgacgcga ccgagggcgc cgtgacgatc gacggggtgg acgtgcggga
      901 gttgacgttc gagtcactcg ccgccacggt gggcgtcgtc tcacaggaga cctatctctt
      961 ccacgcgtcg gttcgtgaga acctgcgctt cgcgcggccg gaggcgaccg atgccgagat
     1021 cgaggaggca gcgcgtgcgg cgcaggtgca cgagctgatc gccaccctgt cggagggcta
     1081 cgacaccgtg gtcggcgagc gcggctaccg cttctcggga ggcgagaaac agcggatcgc
     1141 gattgcgagg acgatcctcc gcaacccgcc cattctcgtc ctcgacgagg cgacttccgc
     1201 cctcgacact cagacggagc ggctcgtcca ggaggcgctc gagcggctgg ccgagggtcg
     1261 cacgacgatt gcgatcgccc atcggctctc caccgtgcgc gacgcggacc agatcgtggt
     1321 gctcgaccgc gggagaatcg ccgagatcgg cacgcatgac gacctgctcg ccgcgggtgg
     1381 gcgctatgca ggactcgtcc ggcgcgacgc cgacacggcg atcgaacgac agcccatcgg
     1441 ctgacggacg cgagcacgta ccccgctgcc atgtcaggcg cggcggacta cggtgtctgg
     1501 tgactgttgc gccgcaagcg actcgaaggg agcagtacgc tcgatgccta tgaccacgcc
     1561 cgagatggtg agcctcgacg aacacggtgt ccaagcaggg ggccgcgtga tccggaaccc
     1621 gacgacaccg atgctctacg agcacgccct ggcgcgaggg gacgggcgtc tggccgccgg
     1681 agggccgctc gcggtggaca ccggcgtgca taccggccgc tcgccgaagg acaagttcgt
     1741 cgtccgcgag ccccaatccg agagccgcat ctggtggggc gccgtcaaca agccgatcgc
     1801 ggaggacaag ttcgagggcc tacgcgcgaa ggtaatcgag catctggcgg gccaggaccc
     1861 cctgtacgtc gtcgacgcgt tcgcgggcgc cgatccggcg caccggatct gcgtgcgaat
     1921 cctcaccggc agtccgtatc acgcgctgtt ctcgaagacg atgttcatcg acccgagcga
     1981 tcaggaactg gcctccttcg agccgacggc cgtcgtcttc catgcccctg aggtggaggc
     2041 cgatccggac accgacggaa cacggtcgag cacgttcgtc tgcctgcatc cgacacgcga
     2101 cgaagtggtg atcggcggga cgttctacgc cggagagatc aagaagtcga tcttcacggt
     2161 gatgaacgac cgcatgccgc tcgagggcgt gttcccgatg cactgctcgg ccaacgtggg
     2221 caaagacggc cgggtggccg tgttcttcgg cctctccgga accggaaaga cgacgctgtc
     2281 ggccgatccc gagcgctcgc tcatcggcga cgacgagcac ggctggggcg acaacggcgt
     2341 cttcaacttc gagggcggtt gctacgccaa ggtcatccgg ctctcggctg aggcggagcc
     2401 ggacatcttc cgcaccaccc atacgttcgg gaccgtcctc gagaacgtcg tcctcgacga
     2461 gcgcggggtg ctcgacctcg acgacgactc gaagaccgag aacactcgtg ccgcctacga
     2521 gctcgagcag atagggaact cgctccccac gaagatggcc ggccatccct ctgcagtcgt
     2581 gatgctgacg gccgacgcgt tcggcatcct gccgccggtc gcccgcctgt cgaaggatca
     2641 ggcgatgtac tggttcctct cgggcttcac cgccaagctc gcggggacgg aaatcggcgt
     2701 gacggagccc cagccgacct tctcctcctg cttcggggct ccgttcctac cgcagccgcc
     2761 ggccgtgtac gccggcatgc tcggcgagaa gctcgaccgg cacggcgcgt ccgtctggct
     2821 cgtgaacaca gggtggaccg ggggtgcgta cggagacggc catcgcatgc cgatcgacgc
     2881 cacgcgtgcg ctcctgcacg cggcgctcgg cggcagcctc gatggcgccg atttccgcat
     2941 cgatccggtc ttcggcttcg aagtgccggt cgccgtgcac ggagtcgacg acgcgctgct
     3001 cgacccgcgt tccacctgga aggacagcga cgcctacgac cggaaggcgc aagagctggc
     3061 cggcatgttc cgggacaact ttgccaagct cgagggcgtc gatccgtcgc tcgccgcagc
     3121 cggccccgct ggctagcagc cagccatggt tggcgcgact cggcgcccgg ctcatcaggt
     3181 agcgtcatac ccgatggagg tgacccacga cgtccttgtc gtcggcgccg gctgtgccgg
     3241 catgcgcgcg gcgatcgagg cgcacgaccg cggtgcgaac gtcggcgtca tctcgaagct
     3301 gcaccctacc cgaagtcact ccggcgcggc cgagggcggc atcaacgcgg cgctcggaaa
     3361 cacggccatg gacagcccgg agacgcatgc cttcgacacc gtcaaaggct ccgactatct
     3421 cggcgatcag gacgcgatcg aggtcttcgc cgccgaggcg ccgggcgaca tctaccagct
     3481 cgagcactgg ggtgcagtct tctcgcgcaa cgaggagggc aagctcgacc agcggccgtt
     3541 cggcgcggcg ggctcgcccc gaacggtctt ctcggccgac atcaccggac acgtgctgat
     3601 ccatgtcctc tacgagcagc tgatgaagcg catggccgag ggaacgaagg tctacgagga
     3661 gttcttcgcc tggcgcctcg tcgtcgagga cggacgctgc accggtgtca tctgctggga
     3721 cctcgtcaac ggcggccttc actacctcac cgggaaagcg gtcatcctgg ccacaggggg
     3781 tgcggggcgg ctctaccgtg cgacgaccaa cgcctacgcc tgcaccggcg acggaatggc
     3841 aatggccctg cgcgccggc
//
LOCUS       DBNGNK010000319         6670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_200290,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000319 DBNGNK010000000
VERSION     DBNGNK010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_200290"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1394
                     /locus_tag="AC150V_09435"
     CDS             <1..1394
                     /locus_tag="AC150V_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012948399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR3763178.1"
                     /translation="AQEAHRRLGAELGKERVGLVTGEERVNEDAPVICSTVEMAPAAG
                     EVLVLDEVQWADDEERGSAWTRLLLAGDYRHILLLGALDALPLVRRAFPTAEIRVFER
                     KLPLEWIGERDLRSLTPGTVVVAFSRKAVLALAGEINRRHAGRVAVLYGAMPLASRRE
                     EIDRFLDGGADVCVATDVLGHGVNLPCETLLFAETTKFDGTARRDLLPWELAQIAGRA
                     GRFGLVERGHVGVLTGVPWADSDPSIVRSALQPHVPLPDGHLGYRVVDEARIRPRLAD
                     LGVDQPRELDAALAAWHRVALREWAHESWLAVESLQPIRMRLAAVQLRLDERRRRISL
                     ADTWTIVNAPVDDDHVELLAALALALAGDRAQRSLLAFLLDPARLRDATLEEAEEAGR
                     VASILRWFALQYAGVGGVTIERAAALEEAAAARVVSRLRVEVESPTVGRCRMCGSTCA
                     PWFPLCDRCYSRN"
     gene            1415..1879
                     /gene="smpB"
                     /locus_tag="AC150V_09440"
     CDS             1415..1879
                     /gene="smpB"
                     /locus_tag="AC150V_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017542494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR3763179.1"
                     /translation="MARAAGSKLIADNRRARYEYDLLERVEAGLVLTGTEVKSLREGR
                     VELGQAFVDFRGGEAWLVGSSIAEYAQGNIQNHEPGRDRKLLLHRRQIDSLYGQVSEK
                     GPTIVPTRLYFKNGKVKIELAVARGRERFDKRRAIAERDAKRQIERELRSRR"
     gene            complement(1941..2861)
                     /locus_tag="AC150V_09445"
     CDS             complement(1941..2861)
                     /locus_tag="AC150V_09445"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3763180.1"
                     /translation="MIDVREIRDEELPRWRELVAAVQPEQTPSVESYLDWRRQSEDMT
                     WLIATVDSQEVGAGLALVGWHTPPGVGIGEAYVAPEYRGQGVGSAIFRELARWVSERG
                     CIELESTVREDDPASVSWVERRGFREVGRSSILALDLTEIEEPVVETPEGVEIVAWAE
                     RPELATGMYQVAREAYPDVPGEKDTDVGTYEEWLSRDMQGSSDEPEGVFAAIADDEVV
                     GYAKLSFDSARPEVAHHDITGVKRGWRGRRIAAALKATEIAWAKRHGYERLETYNEER
                     NEPIRRLNERHGYRIEPGSVTFRAVILGPD"
     gene            2945..3406
                     /locus_tag="AC150V_09450"
     CDS             2945..3406
                     /locus_tag="AC150V_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3763181.1"
                     /translation="MRHVSLTVNGTPYSLDLEPRELLVYVLRERLGLTGTNVGCDTSA
                     CGSCTVLVDGEAVKSCTMLGVQAHGAEITTIEGLAANGELHPVQQAFHEHHALQCGYC
                     TPGMVMAAVSLIDTGAAGSEEEIRSGLEGNLCRCTGYHNIVAAVASAAGGK"
     gene            3408..5756
                     /locus_tag="AC150V_09455"
     CDS             3408..5756
                     /locus_tag="AC150V_09455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3763182.1"
                     /translation="MAATETFIGAPVLRKEDRSLLTGAGTFIDNLTLPGMVWMAVVRS
                     PHPHARVKNVNLDAARAVDGVVAAYSGADLRDDWSSGLPCAWPVTEDMKTPDHFPLTI
                     DEARYQGDGVAVVVADSRTAARDASELVEVDYEPLDVTVDVLESLAEGAPLVHNALGT
                     NECYVWELKPQDVDKAIADADVVVTRRYRQPRLIPNAIEPRGAVAQVGPTGELTMWSA
                     TQIPHVLRFGMSVVLGIPESKVRVIAPDVGGGFGSKLNVYAEEALVGALSARLGRPVK
                     WVEDRTEGYQATIHGRDVVHEMTFAAKKDGTITAVKVDMKAAMGAYLQLVTPGIPILG
                     AWLYAGPYNVPNYGVTITGVFTNTTPTDAYRGAGRPEATYVIERMIDALAAELGMDRL
                     EVRRKNFFSEFPAEMSSGLTIDSGDYHASLDRLVELLDLPAFEAEQQARRDRGDVKQL
                     GVGYSTYNEMCGLAPSRILGAVRYAAGGWESATVRFQPLGSVQIVTGTSPHGQGHETS
                     WAQIAADRLGVDVDDVEVLHGDTAVSQLGMDTYGSRSLAVGGVAVWQATGKVVEKARQ
                     IVAHQLEVAVDDLEYESGTFTVKGSPDQSMTVRDAALQAWIAHDLPDGMEPGMEETAT
                     FDPENFSWPGGAHAAIVEVDTETGDARLVRYVAVDDVGAVVNPQIVDGQVHGGVTQGI
                     AAALFEEGVYDDEGHLLTSNQLTYLVPSAAEVPSFELDRTESRATTNPMGVKGVGETG
                     AIAAAPAVINAVVDALSHLGVTDMALPATPERVWRAIEEAKQ"
     gene            5753..6595
                     /locus_tag="AC150V_09460"
     CDS             5753..6595
                     /locus_tag="AC150V_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3763183.1"
                     /translation="MIPAAFDYEVAESVDHAIELLGSGEDTALLAGGHSLIPAMRLRI
                     ARPARLVDIGRLSDLSYVRDGGDHIAIGALTRHKDVASSDVLRASCPIVSHTAGLIGD
                     PAVRHRGTIGGSLAHGDPAADLPAVTLALGAKFVVRGADGEREIPASEFFTGVFQTAA
                     SGGGMLTEIRVPHLGPSTGWSYQKYHRRAQDWATVGVAALVMRDNGNVVGASIALTNM
                     GATPIAATAAEVALSGSGAAGEAAAHAADGTEPASDQAASSEFRRHLARVLTRRALDE
                     ALAR"
BASE COUNT         1025 a         2276 c         2348 g         1021 t
ORIGIN      
        1 tcgcccagga ggctcatcgc cggctcggcg cagagctggg caaggagcgt gtggggctcg
       61 tcaccggcga ggaacgggtg aacgaggacg ctccggtgat ctgctccacc gtcgagatgg
      121 cccccgccgc gggcgaggtg ctcgtgctgg acgaggtgca atgggccgac gacgaggagc
      181 gaggctcggc ctggacgcgg ctcttgctcg ccggcgacta ccgccacatc ctgctgctcg
      241 gcgcgcttga cgccctgcct ctcgtccggc gcgcgtttcc gacggcggag atacgggtct
      301 tcgagcggaa gctgccgctc gagtggatcg gagagcgtga cctgcgctct ctgacgccgg
      361 gaaccgtcgt cgtcgccttc agccgcaagg ccgtgctcgc cctcgccggc gagatcaacc
      421 ggcgccacgc gggccgggta gcggttctct acggcgccat gcccctcgcg tcgcggcggg
      481 aggagatcga tcgcttcctg gacggcggcg ccgatgtctg cgtggccacc gacgtgctgg
      541 gccacggcgt caacctgccc tgcgagacgc tgctcttcgc agagacgacc aagttcgacg
      601 gcactgcgcg acgcgacctg ctgccctggg agcttgcaca gatcgcgggc cgggcaggcc
      661 gcttcggcct ggtcgaacgg ggtcacgtag gagtgctcac gggcgtgcct tgggcagact
      721 cggatccgtc gatcgtccgg tcggcacttc agccgcacgt gcctctgccc gatgggcatc
      781 tcggctaccg cgtggtcgac gaggcgcgga ttcgccctcg cctggcggat ctcggcgtcg
      841 accagccacg cgagctcgac gcggcgctcg cagcctggca ccgggttgcg ctgcgcgaat
      901 gggcgcacga gagctggctc gcggtcgagt ccctccagcc gatccgaatg cgcttggccg
      961 cagtgcagct gcgtctcgac gagcgcaggc gccgcatctc gctggcagac acgtggacga
     1021 tcgtgaacgc gccggtagac gacgaccacg tggagctctt ggccgccctg gcgctggcgc
     1081 tcgccggcga ccgcgcgcag cggtcgctgc tggctttcct tctcgacccg gcccggcttc
     1141 gcgacgccac gctcgaggaa gccgaggagg cggggagggt ggcgagcatc ctgcggtggt
     1201 tcgcgctgca gtacgcaggt gtcggtggcg tcaccatcga gcgggcggct gctctggagg
     1261 aggcggcggc agcccgggtg gtgtctcgcc tccgggtcga ggtcgagtcg ccgaccgtcg
     1321 gtcgctgccg aatgtgtggc agcacctgcg ccccgtggtt tccgctctgc gaccgctgct
     1381 actcgcggaa ctagcgctac ctacacttcc gcggatggca cgggccgccg gatcgaagtt
     1441 gatcgccgac aaccgtcgcg cgcgctacga gtacgacctg ctcgagcgag tcgaggcagg
     1501 cctcgtcctg accggcacgg aggtcaagtc gctgcgggag ggccgcgtcg agctcggcca
     1561 ggcgttcgtc gacttccgcg gcggcgaggc ctggctcgtc ggctcctcga tcgcggagta
     1621 cgcgcagggg aacatccaga atcacgagcc cgggcgcgac cggaagctgc tgctgcaccg
     1681 gcgccagatc gactcgctgt acgggcaggt gagcgagaag gggcccacga tcgtgccgac
     1741 ccgcctctat ttcaagaacg ggaaggtcaa gatcgagctc gcagtcgcgc gcggtcgcga
     1801 gcggttcgac aagcgccggg caatcgcgga gcgagacgca aaacgccaga tcgagcgcga
     1861 gctgcgctcg cgccgctagc cccgcggctc cgacgtgccg aagacgctcc gccgctcgat
     1921 agcggcgtag ctacgagtcc tcagtcggga cccaggatca cggcgcggaa ggtcaccgag
     1981 ccgggctcga tgcggtagcc gtggcgctcg ttgaggcgac ggatcggctc gttccgctcc
     2041 tcgttgtagg tctccagccg ctcgtagccg tgccgcttcg cccacgcgat ctcggttgcc
     2101 ttgagcgcgg cggcgatccg acggccccgc cacccgcgct tcacgcccgt gatgtcgtgg
     2161 tgcgccacct cgggtcgcgc cgagtcgaac gagagcttcg cgtagccgac gacctcgtca
     2221 tcggcgattg cggcgaagac gccttccggc tcgtcgctcg agccctgcat gtcgcgggag
     2281 agccactcct cgtaggtgcc gacgtcggtg tctttttctc cggggacgtc ggggtaggcc
     2341 tccctcgcga cctggtacat gcccgtcgcg agctcgggtc gctcggccca ggccacgatc
     2401 tcgacgccct ccggtgtctc cacaacgggc tcctcgatct ccgtcaggtc gagcgccagg
     2461 atcgagcttc gcccgacttc ccgaaagccg cgccgctcca cccagctgac gctggcaggg
     2521 tcgtcctcgc gaaccgttga ctcgagctcg atgcagccgc gctccgagac ccagcgggcg
     2581 agctcacgga agatcgccga gccgacaccc tgaccgcggt actcgggagc aacgtacgcc
     2641 tcgccgatac cgacacccgg cggcgtgtgc cagccgacga gcgccaggcc ggcgccgacc
     2701 tcctgactgt cgaccgtggc gatcagccac gtcatgtcct ccgactgccg tcgccagtcg
     2761 aggtagctct cgacggacgg agtctgctcg ggctggacgg ctgccacgag ctcgcgccag
     2821 cgcggcagct cctcgtcgcg gatctcgcgt acgtcgatca tggcgcgaag cgtaacgtgc
     2881 gggttttgtc cacatcggcg ggaggagtag gctcggttgt ccgaccgcga aaggaggccg
     2941 atccttgcga cacgtttcgc taaccgtcaa cggcacccca tattcgctcg atctcgagcc
     3001 gcgggagctg ctcgtttacg tccttcggga gcgcctcggg ctcacgggca cgaatgtcgg
     3061 ctgcgacaca tccgcgtgcg gctcttgcac ggtgctggtg gacggtgagg ctgtcaaatc
     3121 ctgcacgatg ctcggcgtcc aggctcatgg cgccgagatc acgacgatcg agggcctcgc
     3181 cgcaaacggt gagctgcacc ccgtgcagca ggccttccac gagcatcacg cgctccaatg
     3241 cggctactgc actcccggca tggtcatggc cgccgtttcg ctgatcgaca cgggcgccgc
     3301 cgggagcgaa gaggagatcc gctcaggtct cgagggcaat ctttgccgct gcaccgggta
     3361 tcacaacatc gtcgccgcgg tcgcctccgc ggcagggggg aagtgagatg gctgcgaccg
     3421 agacgttcat cggcgcgccg gttctccgca aggaggatcg ctccctgctc accggcgccg
     3481 gcaccttcat agacaacctg acgctgcccg gaatggtgtg gatggcggtc gtccgcagtc
     3541 cgcatccgca tgcgcgagtc aagaacgtga acctggacgc cgcccgcgcg gtcgacggcg
     3601 tcgtcgccgc atacagcggc gccgacctca gagacgactg gagctccggc ctgccgtgtg
     3661 cgtggccggt gaccgaggac atgaagactc cggatcactt cccgctcacg atcgacgagg
     3721 cgcggtacca gggcgacgga gttgccgtcg tcgtagcgga cagccgcacg gctgcgcgcg
     3781 acgcgtcgga gctggtcgag gtcgactacg agccactcga cgtcactgtc gacgtgctcg
     3841 agtcgctcgc ggagggcgcg ccgctcgtgc acaacgcact cggtacgaac gagtgctacg
     3901 tctgggagct caagccgcag gacgtcgaca aggcgatcgc ggacgcggac gtcgtcgtga
     3961 cgcggaggta ccggcagccg cgcctgatcc cgaacgcgat cgaaccgcgc ggcgccgtcg
     4021 cccaggtcgg cccgaccggc gagctgacga tgtggtcggc aacgcagatc ccgcacgtcc
     4081 ttcgttttgg gatgtcggtc gtgctcggga tccccgagtc gaaagtccgc gtgatcgcgc
     4141 ccgacgtcgg cggcggcttc ggctcgaagc tgaacgtgta cgcagaagag gcactcgtcg
     4201 gagcgctctc agcgcggctc ggccgcccgg tgaagtgggt cgaggaccgc acggagggct
     4261 atcaggcgac gatccacggc cgcgacgtcg tgcacgagat gacgttcgcc gcgaagaagg
     4321 acgggacgat cacggccgtg aaggtcgaca tgaaagcggc catgggcgca tacctgcagc
     4381 tcgtcacgcc cgggatcccg atcctcgggg cctggctgta cgcaggcccg tacaacgtcc
     4441 cgaactacgg cgtcaccatc acgggagtct tcacgaacac gacaccgacg gacgcgtacc
     4501 ggggggccgg caggcccgag gcgacgtacg tgatcgagag gatgatcgac gccctcgccg
     4561 ccgagctcgg catggatcgc ctggaggtgc ggagaaagaa cttcttctcc gagttcccgg
     4621 ctgagatgtc gtccggcctg acgatcgact ccggcgacta ccacgcctcg ctcgatcgtc
     4681 tcgtcgagct gctcgacctg ccggcgttcg aggcggagca gcaggcgcgg cgcgatcgcg
     4741 gggacgtcaa gcagctcggc gtcgggtact cgacgtacaa cgagatgtgc gggctcgccc
     4801 cctcgcggat cctcggcgcg gtgcgatacg cggctggcgg ctgggagagc gcaaccgttc
     4861 gtttccagcc cctcgggtcg gtgcagatcg tcacgggcac gtcgccccac ggccagggcc
     4921 acgagacgtc atgggcgcag atagcggccg accggctcgg cgtcgacgtc gacgacgtgg
     4981 aggtgctgca cggcgacacc gcggtgtccc agctgggaat ggacacgtac ggctctcgct
     5041 cgctcgccgt cggcggcgtc gccgtctggc aggcgaccgg gaaggtggtc gagaaggcgc
     5101 gacagatcgt cgcccaccag ctcgaggtgg cagtcgacga tctcgagtac gagagcggca
     5161 cgttcaccgt gaagggctcg cccgaccagt cgatgaccgt gcgagacgcg gctctgcagg
     5221 cgtggatcgc ccacgatctg cccgacggga tggagcccgg gatggaggaa acggcgacgt
     5281 tcgatccgga gaacttctct tggccgggcg gcgcgcatgc agcgatcgtc gaggtggata
     5341 ccgagactgg tgatgcacgg ctcgtgcgtt acgtcgcggt cgacgacgtt ggcgcggtgg
     5401 tgaacccgca gatcgtcgac ggccaggtac acggcggagt gacgcaaggc atcgccgccg
     5461 cactcttcga agagggggtg tacgacgacg agggccatct cctgacgtcg aaccagctga
     5521 cgtacctcgt gccctcggcg gccgaggttc cctccttcga gctcgaccgg acggagagta
     5581 gggcaacgac gaatccgatg ggcgtgaagg gggtggggga gacgggcgcc atcgccgccg
     5641 ccccggctgt catcaacgcg gttgtcgacg ctctctccca tctcggcgtc accgacatgg
     5701 cacttcccgc cacgccggag cgagtgtggc gcgcgatcga ggaggcgaaa cagtgatccc
     5761 tgcagcgttc gactacgagg tcgccgagag tgtcgatcac gcgatcgagc tgctcggctc
     5821 gggtgaagac accgcgctgc tcgcgggcgg gcactcgctg atcccggcga tgaggctccg
     5881 gattgcgcgg ccggcgcggc tcgtcgacat cggtcgtctc tccgacctct cctacgttcg
     5941 cgacggaggc gaccacatcg caatcggcgc tctcacccgc cacaaggacg tcgcctcgag
     6001 cgatgtcctg cgggcgagct gtccgatcgt ctcccacacc gcgggcctga tcggtgatcc
     6061 cgcggtgcgt caccgcggca cgatcggtgg atcgctcgcc cacggcgatc cagccgccga
     6121 tctaccggcg gtcacattgg cgctcggcgc gaagttcgtc gtccgcggag cagacggaga
     6181 gcgcgagatc cccgcctccg agttcttcac cggcgtcttc cagacggccg ctagcggggg
     6241 cgggatgctg accgagatcc gcgttcctca cctcgggccc tcgaccggct ggtcgtacca
     6301 gaagtaccac cggcgtgccc aggactgggc gacggtcggc gtggccgctc tcgtcatgcg
     6361 ggacaacggc aacgtcgtcg gtgcctcgat cgcgctcacc aacatgggcg cgacgccgat
     6421 cgccgcgacg gctgcagagg ttgcactgtc ggggagcgga gccgccgggg aagcggcggc
     6481 ccacgcggcg gacggtaccg agccggcgtc cgatcaggcg gccagttccg agttccggcg
     6541 gcacctcgcg cgggtgctca cccgccgggc gctcgatgag gcgctcgcgc gctgagaggc
     6601 gagctcctcg tccccgaaga ccgctccgcc gcggcgccct cgggcccgcg gcggagcacc
     6661 cacgcgacag
//
LOCUS       DBNGNK010000320        10250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1201422,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000320 DBNGNK010000000
VERSION     DBNGNK010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10250
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1201422"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..763
                     /locus_tag="AC150V_09465"
     CDS             143..763
                     /locus_tag="AC150V_09465"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR3763184.1"
                     /translation="MAERLTVGVAARLSRYLQVLTQAKKMGKDRISSQEIAEYTNINA
                     TQIRRDLSAFGKFGKRGVGYNIDSLLGEIRTILRTQGQHNIALVGAGRLGEAIASSPI
                     FAEHGITIAAVFDIDPAKVGSSINGVTVSSPDQLERIAHENNIIVGVLAVPAENAQIA
                     ADTLVGAGVKIIFNYSQALLDTPPDVQVHTSNPAVELLYALYFHLT"
     gene            781..2025
                     /locus_tag="AC150V_09470"
     CDS             781..2025
                     /locus_tag="AC150V_09470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR3763185.1"
                     /translation="MRIVLWHGYLLGGTGSNVYSRQLAREWSRAGHDVTVLSQEPHPE
                     LYDLGGATTVRPDVHGLLPVFVLDDYEGYTVKLVQDCSRDELERWVEHNVSAIRALSP
                     ADLVFANHVLLGGPVGAASGCRYAVKAHGSELEYSMRGNSELSEWGRESLAGAAAVYV
                     GSAHVRDVLEEVVGAVDRVYEVPPGVDVELWRPQPRERALEALVEEARRDRPNPANAN
                     ERLPDEGNADRLASFLAEDEPTVVYFGKLLYNKGVHVLLEAMRSVGARTVVVGFGDYR
                     EELEAMAGEGTLFTGPLEHRHLSHLLALADASVVPSIFPEAFGMVAAESAAAGCPPLV
                     ARHSGLAEVVAGLEAEYPSSLGHLASFRTGDVAELAAKLRKLLSLPDQDRHAVSEGAR
                     RAAVERWSWASVARDLLEPVGL"
     gene            2060..3229
                     /locus_tag="AC150V_09475"
     CDS             2060..3229
                     /locus_tag="AC150V_09475"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR3763186.1"
                     /translation="MGDSQRVSHDQLLREARERFESGRDFTIAVEEEFALLDPATHDL
                     VNRFEDVRAAVAGTPLEPHLVGELIASEVEVKTGSCDGFADVPGRIVERRAQLAALVE
                     PLGIELGTTGTHPWAEWKEQRIIDTPHYRRNDELLRYVVWRNNTFGLHVHVGIRGADR
                     AVRVHDALRLFLPELLAISASSPFVEAVNTGLHSARTQIFTRFFPRCGVPDAYGSWDA
                     FERYVRFLYETGSIDEHTQLWWSVRPHLAFPTVEIRICDIQPDAAESQALAALSASVV
                     ARIARAVDDNEPLPDVPPRLIEENFWRAIRSGLAGELIDFEQERSIPARARIEGLIEW
                     VRPAAEEIGAASWLAVPERNAAERQIARYEEGASLEEIYAEYVGVTERPVGSPAE"
     gene            3335..5383
                     /locus_tag="AC150V_09480"
     CDS             3335..5383
                     /locus_tag="AC150V_09480"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018077261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR3763187.1"
                     /translation="MEFFKDHAVLFALICSGVAIVYGLYLIYWLLSQPQGNERMREIA
                     RAIQEGADAYLRKQYTTIAAVAVAPFLLLGFYNQLGWGVACGFLIGAALSAAAGFIGM
                     NVAVRSNLRTAEAAREGVGPGLKVAFRAGSVTGLLVVGLGLLGVAGYYWVLTDWFGND
                     PDSAVFDLVGLAFGGSLISVFARLGGGIYTKAADVGADLVGKIEAGIPEDDPRNAAVI
                     ADNVGDNVGDCAGMAADLFETYAVTAVAVMLIGTSFNVAQELWLYPLALGGVSILASV
                     VGTFFARLGRGANAIMNALYKAVLVATVLSAAGFIPITMAYDGGTFSFWELYGPALIG
                     LAITFLLVAITEYYTGTRWHPVKLIARSSQTGHATNIISGLAVGMQATVLPVIVIAAG
                     IVGAYYAAGQELYGIGVAVMAQLSMTGLIVALDAYGPVTDNAGGIAEMADLPESVRGV
                     TDPLDAVGNTTKAVTKGYAIGSAGLAALILFDEYVRGLGDEGLDAVFSLENPWVIAGL
                     FIGGLMPFLFASLAMQAVGRVGGEVVEEVRRQFREDPGIMEGTSRPDYSRAISIVTGS
                     AIKEMLAPAMIPIVIPIVVGIIKPEMLGGLLIGTIVTGIFLAIAMTSGGGAWDNAKKL
                     IEDGAYGGKGSEAHAAAVTGDTVGDPYKDTAGPAINPMIKIANIVAILMIPIIASIHG
                     "
     gene            5460..5894
                     /locus_tag="AC150V_09485"
     CDS             5460..5894
                     /locus_tag="AC150V_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3763188.1"
                     /translation="MKVVVIGCGRVGSGVATQLAGEGWDVTVVDEKQEAFGRLSEEWH
                     GRTVVGHGMDIDILLEAGAEHADAAVVATNGDNTNLVVGQVLQKRFEIGCVVVRVLDP
                     ARADFYAQRGLRTVCPTQTAVSILTDEVRAIDPSPLEPSGAA"
     gene            5884..6564
                     /locus_tag="AC150V_09490"
     CDS             5884..6564
                     /locus_tag="AC150V_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR3763189.1"
                     /translation="MPRKGPSTALIAGGGKVGANLARSLLRMGHQVTMIEQARGRAVR
                     LRDEFGDSVRHGDATELYVLERAGIDAPPDLVLAVTGDDEDNLLIAQVAKEGYGVKKV
                     IARVNDPRNQEHFDLLGITETVCATAGILGLVEHEVPEHGLVRLLELRSDGLEVVEVQ
                     IDPDSPAAGKRVAGLKLPDSSRLISVMRHGRVEAAEDSTVLRPGDQVLALLRPEGEDA
                     LRRALVGT"
     gene            complement(6566..8128)
                     /locus_tag="AC150V_09495"
     CDS             complement(6566..8128)
                     /locus_tag="AC150V_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004572615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3763190.1"
                     /translation="MAAPDRGGGKAGADRAPRIGPPARFAVDIRGSAGLVGRLVKYLS
                     IATLFPLALALGYGESPWPFVGTLLIAGAVGLALERLGGSAGGVGFREGYFVVSLTWL
                     LAAAVGALPYLLSGEAQLANPVDAYFESMSGFTTTGSSVLTDIEGLDRSLLMWRQFTQ
                     WLGGMGIIVLAIAVLPRLRVGGRQLLETELPGPDVESLGERIRQTAQHLWLLYVALTA
                     ALALILTLVGVSGVDDRMGLYEAVAHAFTTMPTGGFGTFNRSVQPFSATTEWVLVLFM
                     VIAGINFALMYRAFARRRPALFARDEELRVYLLALAVGAAIVVGVLYTENIAGSHHAL
                     RHGAFQAVSIVTTTGYASLDFNGWTTLALITLVGLMFVGGSAGSTAGSVKVIRHLLLA
                     KTLRREVRLTVRPELVEPVRFNGSVVDEQTLRAVIGFALLYLGLFIAGAGVLALDSAI
                     QGPTLNPLQTIAAAATTLGNVGPGLGPAGPMDSFATFSDVSKLTLIALMWLGRLEVIP
                     VLVILTRRYWRV"
     gene            complement(8130..9590)
                     /locus_tag="AC150V_09500"
     CDS             complement(8130..9590)
                     /locus_tag="AC150V_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004970960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR3763191.1"
                     /translation="MGTLLKYLSFAALFPAAVALAYGEPPWPFLIAFAVGLASGYALE
                     RLGRATSSAVGSREGYLVVALTWLLAAFFGAIPLLLAGEAQLDRPIDALFEAMSGFTT
                     TGATLVTDIESLDHSVQMWRQFMQWLGGIGIIVLAIAVLPRLRVGGRQMLESELPGPE
                     VDQLGERIVRTAQRLWILYVSLTAAEALILSILGWTGLDDKMSVYEAIAHAFTTIPTG
                     GFSTENGSIGAFGAVTQWVIIGFMALAGVNFALIWRALVRRQPGAAARDDEFRLYAVL
                     LAVASSVLVVELATESLAGSDEPVRNGVFQAVSIMTGTGYATVDFASWTTLAFMTLVA
                     LMFVGGAAGSTTGSVKVVRHLLIGRSLRRELRQTVHPELVVPVRLNGSVIDERTLRAL
                     VSFTLLYVATFVIGAGVIAIDSAIQGPDLVAVDAISASAATLGNVGPALGPAGPLDSY
                     EPFSDVSTVAMTLLMWLGRLELIPVIVLLSRRYWRL"
     gene            complement(9665..>10250)
                     /locus_tag="AC150V_09505"
     CDS             complement(9665..>10250)
                     /locus_tag="AC150V_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR3763192.1"
                     /translation="ARTGVRLIEPSRERAREIAEELPSVRVYHATGLDPEFLERERIG
                     LTQAAVFAMREDAKNLYAATLARVHNVPFTIAISQQKEARDVYEMAGVDVTVDPRQVT
                     AEEIVRFAHDPRTQQIAMIEGDRFEVLDIATKPDSEYVGLRFRDMPIRGALIGAIVRD
                     GRALFPRGDDVLESGDRVIVFTESERVPEVERAL"
BASE COUNT         1682 a         3446 c         3538 g         1584 t
ORIGIN      
        1 aggcgtttac gttcgaaatc gaccccgacg agctgcgcga gcaactcgtc taggccggcc
       61 gcctgccggt tccctccgca gggatgggcg gggcgttggc agaatgtgac gcgttgtcac
      121 aaactcggag caaaggggag agatggcgga acggctgacg gtcggtgtcg cggcgcggct
      181 gagccggtac ctacaggtgc tgactcaggc gaagaagatg ggcaaggaca ggatttcctc
      241 acaggagatc gcggagtaca cgaacatcaa cgcgacccag atccggcgtg atctctccgc
      301 cttcgggaag ttcggcaagc ggggggtcgg ctacaacatc gactcgctcc tcggtgagat
      361 cagaacgatc ctgcgcacac agggtcagca caacattgcg ctcgtcggag caggaaggct
      421 cggcgaggcg atcgccagct cgccgatctt cgccgagcac gggatcacga tcgctgcggt
      481 cttcgacatc gatccggcga aggtgggatc gtcgatcaac ggcgtcacgg tcagctcgcc
      541 cgaccagctc gagcggatcg cgcacgagaa caacatcatc gtcggcgtac tcgccgtccc
      601 ggccgagaac gcacagatcg cggccgacac cttggtcggt gcgggagtca agatcatctt
      661 caactactcg caggccttac tcgacacacc ccctgacgtc caggtgcaca cctccaatcc
      721 cgcggtcgag cttctctacg cgctctactt ccacctgacg tagagggccg agctgccgtc
      781 gtgaggatcg tcctctggca cggctacctg ctcggcggca ccgggtccaa cgtctattcg
      841 cggcagcttg ctcgcgagtg gtcgcgggcg ggccacgacg tgacggtact gagccaggag
      901 ccgcatccgg agctctacga cctcggcggg gcgaccactg tgcggccgga cgtgcacggc
      961 ctgctgccgg tgttcgtcct cgacgactac gagggctaca ccgtgaagct cgtgcaggac
     1021 tgctcccgcg acgagctcga gcgctgggtg gagcacaacg tctcggcgat ccgcgcgctc
     1081 tcaccggccg atctggtctt cgccaatcac gtgctgctcg gcggtccggt cggtgctgcg
     1141 agcggctgcc gctatgcggt caaggcacac ggctccgagc tcgagtactc gatgcgaggc
     1201 aattcggagc tgtccgagtg gggacgcgag tcccttgccg gagcggctgc ggtctacgtc
     1261 ggctccgcgc acgtccgcga tgtgctcgag gaggtcgtcg gcgcggtcga ccgcgtgtac
     1321 gaggtgccgc cgggcgtgga cgtcgagctg tggcgcccgc agccgcgtga gcgcgcgctc
     1381 gaggcgctcg tcgaggaagc ccgccgcgac cggccgaacc ccgccaacgc caacgagcgt
     1441 ctcccagacg aaggcaacgc cgaccgcttg gcgtcgttcc tcgcagagga cgagcccacc
     1501 gtcgtctact tcggaaagct gctctataac aagggtgttc acgtcctgct cgaggccatg
     1561 cgatcggtcg gcgcgcgaac agtcgtcgtc ggcttcggcg actaccgaga ggagctcgag
     1621 gcaatggccg gcgagggcac gctgttcacc ggacccctcg agcaccgaca ccttagtcac
     1681 ctgctcgccc tcgccgacgc atccgtcgtc ccgtcgatct tccccgaggc gttcggcatg
     1741 gtggccgccg agtcggctgc ggccggctgc ccgccgctcg tcgcacgtca ctcgggtctc
     1801 gccgaggtcg ttgctggcct ggaggccgag tacccgtcca gtctcggcca tctggcgagc
     1861 ttccggacgg gagacgtggc cgagctcgcg gccaagctgc gcaaactgct gtcgcttccc
     1921 gaccaggacc ggcacgctgt ctcggaagga gcacggcggg cggcagtgga gcgctggagc
     1981 tgggccagcg tcgcgcgcga cctcttggag ccggtcggct tgtgatcgcg gccgcgttcc
     2041 gccgataacg ttccgcacgg tgggagactc acagcgcgtt tcgcacgacc aactccttcg
     2101 cgaagcgcgc gaacgattcg agtccggtcg ggacttcacg atcgccgtcg aggaagagtt
     2161 cgcgctcctc gacccggcga cacacgatct cgtgaaccgc ttcgaggacg tcagagccgc
     2221 cgtcgcaggc actccgctcg agcctcacct cgtcggtgag ctgatcgcgt cggaggtgga
     2281 ggtcaagacg ggttcctgcg acggtttcgc cgacgtccca gggcgcatcg tcgagcgacg
     2341 cgcacaactc gctgcactcg tcgagcccct cggaatcgag ctcggcacga ccgggacgca
     2401 tccctgggcg gaatggaaag agcagcgaat catcgatacg ccgcactacc gccggaacga
     2461 cgagctgctc cgttacgtcg tctggcgcaa caacaccttc gggctccacg tccacgtcgg
     2521 catcaggggc gccgaccgcg cggttcgggt gcacgacgcc ttgcgactct tcctgcccga
     2581 gctgcttgcg atctccgcca gctcaccctt cgtcgaggcc gtgaacaccg gcctccactc
     2641 ggcgcggacg cagatcttca cacgcttctt cccacgttgc ggcgtccccg acgcctacgg
     2701 cagctgggac gcattcgagc gctacgtgcg gttcctctac gagaccggat cgatcgacga
     2761 gcacacgcag ctgtggtgga gcgtgcggcc gcatctcgcc ttcccgaccg tcgagatccg
     2821 catctgcgac atccagccgg atgcggccga gagccaagcg ctcgccgcgc tctcggcgtc
     2881 ggtcgtcgcg cgtatcgcgc gggcggtcga cgacaacgag ccgctgccgg atgtcccgcc
     2941 ccgcctgatc gaggagaact tctggcgggc gatccgctcc gggctcgcgg gggagctgat
     3001 cgacttcgag caggagaggt cgattccggc acgggcgcgc atcgagggtc tgatcgagtg
     3061 ggtgcggccg gccgccgagg agatcggcgc ggcgtcctgg ctcgccgttc cggagcggaa
     3121 cgcggccgag cggcagatcg cccgttacga ggaaggcgcg tctctggagg agatctacgc
     3181 ggagtacgtc ggcgtcaccg agcgccccgt cggctcaccg gccgagtagc catgagcgcg
     3241 ctccccgggc gcgagtctga cgcttgtgat acataatccc ccgcgccgag ggcactcggc
     3301 gctcattttg tgacgggcag gatccggagg gcggatggaa ttcttcaaag accacgcggt
     3361 gttgttcgcg ctcatctgtt caggtgtcgc gatcgtctac ggcctctacc tcatctactg
     3421 gctgctcagc cagccgcagg ggaacgagcg catgcgcgag atcgcacgcg ccatccagga
     3481 gggagccgac gcgtacctgc gcaagcagta cacgacgatc gccgccgtcg cggtcgcgcc
     3541 gttcctcctg ctcggcttct acaaccagct cggctgggga gtcgcatgcg ggttcctcat
     3601 cggcgccgct ctctctgctg ctgccggttt catcggcatg aacgtcgcgg tgcgctcgaa
     3661 cctccgcacc gcagaggcgg ctcgtgaggg agtcgggccg ggcctcaagg ttgcctttcg
     3721 tgccgggtcc gtcacaggcc tgctcgtcgt cgggctgggg ctgctcggcg tggccggcta
     3781 ctactgggtt ctgacggact ggttcggcaa cgatccggac tccgccgtct tcgacctcgt
     3841 cgggctcgcg ttcggcggct cgctgatctc ggtcttcgcc cggctcggcg gcggcatcta
     3901 cacgaaggcg gccgacgtcg gtgccgacct cgtcggaaag atcgaggctg gtatcccgga
     3961 agacgacccg cgcaacgccg ccgtcatcgc ggacaacgtc ggtgacaacg tcggcgactg
     4021 cgccggcatg gccgccgacc tgttcgagac gtacgccgtg accgcggtcg ccgtcatgct
     4081 gatcgggacg tcattcaacg tggcccagga gctctggctc tacccgctcg cactcggcgg
     4141 cgtctcgatc ctggcgtcgg tggtcggcac gttcttcgcc cggctcggcc gcggcgcgaa
     4201 cgcgatcatg aacgccctgt acaaggccgt cctcgtggcg accgtcctgt ccgccgctgg
     4261 cttcatcccc atcacgatgg cctacgacgg cggcaccttc agcttctggg agctctacgg
     4321 gccggccctg atcgggctcg ccatcacctt cctgctcgtt gcgatcaccg agtactacac
     4381 ggggacgcgc tggcacccgg tgaagctgat cgcgcgctcg tcacagacgg gccatgcgac
     4441 gaacatcatc agcgggctgg ccgtcggcat gcaggcgacg gttctgcccg tcatcgtcat
     4501 cgccgccggc atcgtcggcg cctactacgc ggctggtcag gagctgtacg gaatcggcgt
     4561 cgcggtgatg gcgcagcttt cgatgacagg gctgatcgtt gcgctcgatg cctacgggcc
     4621 ggtgaccgac aacgccggcg gaatcgcaga gatggccgat ctgccggaat cggtgcgagg
     4681 cgtcaccgac ccgctcgatg ccgtgggcaa cacgacgaag gccgtgacca aggggtacgc
     4741 gatcggctcc gccggtctgg cggcgctgat cctcttcgac gagtacgtac gcgggctggg
     4801 cgacgaaggg ttggacgcgg tcttcagcct cgagaacccg tgggtgatag ccggtctctt
     4861 catcggcggc ttgatgccct tcctgttcgc ctcgttggcg atgcaggccg tcggccgtgt
     4921 cggcggcgag gtcgtcgagg aggtgcgacg tcagttccgc gaggacccgg gcatcatgga
     4981 gggcacgtcg cggccggatt actcgcgggc gatctcgatc gtcacggggt ctgcgatcaa
     5041 ggagatgctc gctccggcga tgattccgat tgtgatcccg atcgtggtgg ggatcatcaa
     5101 gccggagatg ctcggcggcc ttctcatcgg cacgatcgtg accggcatct tcctggcgat
     5161 cgcgatgacg tcgggcggcg gcgcgtggga caacgcgaag aagctgatcg aggacggcgc
     5221 gtacggcggc aagggctcgg aggcgcacgc cgccgccgtc accggcgaca cggtgggcga
     5281 cccgtacaag gacaccgccg gtccggcgat caacccgatg atcaagatcg cgaacatcgt
     5341 cgcgatcttg atgattccga tcatcgcctc gattcacggc tgacgcgtcg agcggggagg
     5401 gcggcggccg cgtgccggct ctccccgcta cgcgatgatg tcgttactct gagagcgacg
     5461 tgaaggtcgt cgtcatcgga tgtggccggg tcggctccgg cgttgcgacg cagctggccg
     5521 gcgagggctg ggacgtgacg gtcgtcgacg agaagcagga agcgttcgga cgcctctccg
     5581 aggagtggca cgggcgcacc gtcgtcggcc acggcatgga catcgacatc ctgctcgagg
     5641 cgggcgccga gcacgcggac gccgccgtcg tcgcgacgaa cggtgacaac accaatctgg
     5701 tcgtcgggca ggtgctgcag aaacggttcg agatcggctg cgtcgtcgtg cgcgtgctcg
     5761 atcccgcccg agccgacttc tacgcgcagc gcggcctgcg cacggtctgc cccactcaga
     5821 ctgccgtctc gatcctcacc gacgaggtgc gggcgatcga cccgagcccg ctggagccga
     5881 gcggtgccgc gtaagggccc gagcaccgcg ctcatcgcgg gcggcggcaa ggtcggcgcc
     5941 aacctggcgc gatcgcttct ccgcatgggg caccaggtga ccatgatcga gcaggcacgt
     6001 ggtcgagccg tccgcctgcg cgacgagttc ggcgacagcg tccgccacgg ggacgcgacc
     6061 gagctctatg tcctcgagcg ggccggcatc gacgctccgc ccgacctcgt cctcgccgtg
     6121 acgggtgacg acgaggacaa tctcctgatc gcccaggtcg cgaaggaggg ctacggcgtc
     6181 aagaaggtga tcgcccgcgt caacgacccg cgcaatcagg agcacttcga tctgctcggc
     6241 atcaccgaga cggtgtgcgc gacggcggga atcctggggc tcgtcgagca cgaggttccc
     6301 gagcacggcc tcgtccggct cctcgaactg cggagcgacg ggctcgaggt cgtcgaggtg
     6361 cagatcgatc ccgactcgcc ggcggccggc aagcgcgtgg cgggcctgaa gctccccgac
     6421 agctcgcggc tcatctccgt gatgcgccac ggtcgggtcg aggcggccga ggactcgacg
     6481 gttctgcgcc ccggcgacca ggtgctcgcg ctcctgcgcc cggagggcga ggacgcgttg
     6541 cggcgcgcgc tcgtcggaac ctgactcaca cgcgccagta gcgtcgggtc aggatgacca
     6601 ggaccggaat cacctccagt cgcccgagcc acatgagcgc gatcagggtg agcttggaga
     6661 cgtcgctgaa ggtcgcgaac gagtccatcg ggcccgccgg cccgagcccc gggccgacgt
     6721 tgccgagcgt ggtcgccgcg gccgcgatcg tttgcagcgg gttcagcgtc ggcccctgaa
     6781 tcgccgagtc gagcgccaga acccccgctc cggcgatgaa caggccgagg taaagcagcg
     6841 cgaagccgat gactgcgcgg agggtctgct cgtcaaccac cgagccgttg aagcgcaccg
     6901 gctcaacgag ctcgggtcgg accgtcagcc ggacctcgcg gcggagcgtc ttcgcgagca
     6961 gcaggtgccg gatcaccttc accgagcccg cggtcgagcc ggccgagccg cccacgaaca
     7021 tcaggccgac gagcgtgatc agcgccagtg tcgtccagcc gttgaagtcg agcgaggcgt
     7081 aaccggtcgt cgtcacgatc gacaccgcct ggaacgcgcc gtggcggagg gcgtggtggc
     7141 tgcctgcgat gttctccgtg taaagcactc cgaccacgat cgcggcgccg acggcgagtg
     7201 cgagcaggta gacgcggagc tcctcgtccc gggcgaagag cgccggccgg cgacgcgcaa
     7261 aggcccggta catgagcgcg aagttgatcc cggcgatcac catgaagagc accaagaccc
     7321 actccgtcgt cgcagagaag ggctgcacag agcggttgaa ggtcccgaag ccccccgtcg
     7381 gcatggtggt gaaggcgtgc gccacggcct cgtagaggcc catccggtcg tcgaccccgc
     7441 tgaccccgac cagggtgagg atcagggcca acgcggccgt cagggcgacg tacaggagcc
     7501 acaggtgctg ggccgtctgg cgaatccgct cgcccagtga ctcgacatcg ggtccgggca
     7561 gctctgtctc gaggagctgc cgcccgccga ctcgcaggcg cgggagcacc gcgatggcga
     7621 ggacgatgat ccccatccca cccagccatt gggtgaactg acgccacatg agcagcgacc
     7681 gatcgagccc ctcgatgtcc gtgaggacgg acgagccggt cgtggtgaac cccgacatcg
     7741 actcgaagta cgcgtcgacc ggattggcca gctgcgcctc tcccgagagc aggtagggca
     7801 gtgcgcccac cgcagcagcg agcagccagg tcagggagac gacgaagtaa ccctcacgga
     7861 atccgacgcc gcctgccgag cccccgagcc gctcgagtgc caggccgacc gccccggcga
     7921 tcagcagcgt gccgacgaac ggccacggcg actccccgta ccccagcgcg agggcgagag
     7981 ggaacagtgt ggcgatcgag aggtatttga cgagccggcc gacgaggccg gccgatcctc
     8041 ggatatcgac ggcgaaccgg gcaggcgggc cgattcgcgg ggcccggtcg gccccggcct
     8101 tcccgccccc gcggtccggt gcggccatct cacaacctcc agtagcggcg cgagaggagg
     8161 acgatcacgg gtatcagctc gagacggccg agccacatca gaagcgtcat cgcgacggtc
     8221 gagacatcgc tgaacggctc gtaggagtcg agcggaccgg ccgggcccag cgcgggaccg
     8281 acgttcccga gtgtcgcggc ggacgccgag atcgcgtcga cggcgaccag gtcgggacct
     8341 tgaatcgctg aatcgatggc gatcaccccg gctccgatca cgaaggtggc gacgtacagg
     8401 agggtgaaag acaccaacgc tcgcagcgtg cgctcgtcga tcacggaacc gttgaggcgc
     8461 accgggacga cgagctcagg gtggacggtc tggcgaagct cgcggcgcag cgagcgaccg
     8521 atcaagaggt gccggaccac cttgaccgat ccggtcgtcg aacccgcggc gcccccgacg
     8581 aacatcagag cgaccagcgt catgaatgcg agggtcgtcc agctcgcgaa gtcgaccgtc
     8641 gcgtagccgg tgccggtcat gatcgagacg gcctggaaga cgccgttccg aaccggctcg
     8701 tcgctccccg cgaggctctc cgtcgccagc tcgacgacga gaaccgagga ggcgacggcg
     8761 agcaagacgg cgtagaggcg gaactcgtcg tcgcgtgcgg ctgctccggg ttgacgtcgg
     8821 accagcgcgc gccagatcag cgcgaagttg acgcccgcga gcgccatgaa gccgatgatc
     8881 acccactgcg tgaccgcgcc gaacgcgccg atcgacccgt tctcggtcga gaagccaccg
     8941 gttgggatcg tcgtgaaggc gtgggcgatc gcctcgtaga cgctcatctt gtcgtcgagt
     9001 ccggtccacc cgaggatcga gagaatgagc gcctcggctg ccgtcaggga gacgtacagg
     9061 atccagaggc gttgcgccgt ccggacgatg cgctccccga gctggtcgac ctccgggccg
     9121 ggcaattcgg actcgagcat ctggcggccg ccgacgcgca ggcgtggcag cacggcgatc
     9181 gcgaggacga tgattccgat gccgccgagc cactgcatga actgacgcca catctgaacc
     9241 gagtgatcga gactttcgat gtcggtgacg agggtcgcgc cggtcgtcgt gaaacccgac
     9301 atcgcctcga acagggcatc gatcggccgg tcgagctgcg cctcgccggc cagcagtagt
     9361 gggatggcac cgaagaacgc ggcgagcagc caggtgagcg cgacgacgag gtagccttcg
     9421 cggctgccga cggcggagct cgtcgcgcgt ccgaggcgct ccaacgcgta gccggatgcg
     9481 aggccgactg cgaaggcgat caggaaaggc caggggggct ctccgtacgc gagcgccacc
     9541 gcggccggga agagcgctgc gaagctcagg tacttgagga gcgttcccac gaggccgagg
     9601 gccccgcgga tgtccacgcc gatcttgacc ggccgaccga tccggcgcgg cgcggtggcg
     9661 gcggtcacag cgcgcgttcg acttcgggta cgcgctccga ctctgtgaag acgatcaccc
     9721 gatctcctga ctccaggacg tcgtcgccgc gcgggaagag cgctctgccg tcacgcacga
     9781 ttgctccgat cagcgcaccc ctgatcggca tgtcccggaa gcgcaggccg acgtattcgc
     9841 tgtcgggctt cgtcgcgatg tcgagtacct cgaagcggtc gccttcgatc atcgcgatct
     9901 gctgtgtccg cgggtcgtgc gcgaagcgca cgatctcctc ggccgtcacc tgccgcgggt
     9961 cgacggtgac gtccacgccg gccatctcgt agacgtcgcg cgcctctttc tgctgggaga
    10021 tggcgatcgt gaacgggaca ttgtggacgc gcgcgagtgt cgccgcgtag aggttcttgg
    10081 cgtcctcccg catggcgaag actgcggcct gcgtcagccc gatccgctcg cgttcgagga
    10141 actccggatc gagcccggtg gcgtggtaga cgcgcacgct cggtagctcc tctgcgatct
    10201 cacgggctcg ttcgcgggac ggctcgatca ggcgcacgcc cgtcctcgcc
//
LOCUS       DBNGNK010000321         2139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1919923,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000321 DBNGNK010000000
VERSION     DBNGNK010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1919923"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..480)
                     /locus_tag="AC150V_09510"
     CDS             complement(202..480)
                     /locus_tag="AC150V_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763193.1"
                     /translation="MRELDPADVSRFLEHISREHERRQSRRKPEERKPVSQATLAAIG
                     IALELAARKAGSLAAALGIAAVGVATTIATSGSDRMNATERLLGSRLP"
     gene            complement(1050..>2139)
                     /locus_tag="AC150V_09515"
     CDS             complement(1050..>2139)
                     /locus_tag="AC150V_09515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006942477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR3763194.1"
                     /translation="AEQEEAVLTSEITAASSRVREVQGQVDAEQQRLYGLEAQLASER
                     GKLARLNERFESQTAQLRAFEAEHVTAVAHLERRVREIYMEDTPDVLSFVLGAVSFGD
                     LLDNIELLNDIGRQDARIAARVADGEQKTKKVRQETAETRVEVRATTLAVAAQVAEQR
                     AVRDRLVATRNELVAANDARERTLALVRADNANHAAEIDALQTESSALAAQIRAAQAA
                     ASSASSAASSAPVPLASSGSGISSSGFIFPVAGPVTSGFGPRGGRMHEGLDIIAASGT
                     PVVASASGTVIRSNYGSGYGNVVVIDHGNGLTTTYAHNSSLVASAGQTVSQGQLIAYV
                     GSTGNSSGPHVHLEFRINGTAVDPFGYL"
BASE COUNT          307 a          703 c          727 g          402 t
ORIGIN      
        1 gcaaggtcgt cgagttgtga acgcctccta aggccgtcta gctcccacgc cgggcggccc
       61 tcactccgag tgcgcacgcg ctgacgatga ggaggacgcc caggagcatg gggccgggcg
      121 cgtcgtcggc tcccccgagt ggtgagctcc gcggtcgcag agggctacgc agccgaaaat
      181 cctgtctcgc ggatccactc ctcacggaag ccgcgagccg agaagtcgct ccgtggcgtt
      241 catcctgtcg ctgccgctcg tggcgatggt cgtggcgacg ccgacggcgg cgatgccgag
      301 cgcggccgcg aggctccccg ccttcctcgc tgccagttcg agcgcaatac cgatagccgc
      361 cagcgtcgcc tgcgagaccg gcttgcgctc ctcgggcttc cgccgtgact gtctgcgctc
      421 gtgctcgcgg ctgatgtgct cgaggaagcg cgagacgtcc gccgggtcga gctcgcgcac
      481 cttcgtcttg ccgaaggtcg ccttccgctt agtcgagggt tcccttgagc acgcgcaggt
      541 actcgtcctt gcgccctgct gttcggagcg agccgagctt ctagttcgct caacttcgat
      601 ccagaagaag aactacaatt tgcaggaatt ttctgatgcg cccggcagga ttcgaacctg
      661 cgacccgcgg attagaagct caagacgcgg gtctcggctc gtctcggccg gtctggcgaa
      721 tcggcctgtt cacgccgcaa ccgcctccca cgccgtctgg cgtctcggct gatctcggtg
      781 ggtcgtgttt ccactctgtt gccacccccg agcgcgtcga ggagcgcggg ctcgggcacg
      841 tcgtcgtcga ggagatgcac gtagcggcgc agcgtgaagc ccgcgtcgga gtgcccgaga
      901 tgcgtcggtt tgcgacctag gcctttaggg ctcggtccag tgttcaagct cggttcctcg
      961 cccggggaga cggcatcctt cccctcggta gcgaaccaat cgtcctcggc gggcaagacg
     1021 cgttcggcgt ctccctcgcg tcggcgcatc tagaggtagc cgaaggggtc gacggcggtt
     1081 ccgttgatcc ggaattcgag atggacgtgt gggccgctcg agttgccggt gctgccgacg
     1141 taggcgatga gctggccttg tgagacggtc tggcccgcgc tggccacgag cgatgaattg
     1201 tgtgcgtacg ttgtcgtgag gccgtttccg tggtcgatga cgacgacgtt tccgtaaccg
     1261 ctgccgtagt tcgaccgaat gaccgtgccc gacgcagaag cgacgaccgg ggtgccggat
     1321 gcggcgatga tgtcgaggcc ttcgtgcata cgccctccgc gcgggccgaa gccgctcgtg
     1381 acgggtcccg cgacggggaa gatgaagccc gaggacgaga tcccggagcc gctcgaggca
     1441 agcggtactg gggcggagct ggccgctgaa gaggccgagg acgctgcggc ctgcgctgcc
     1501 ctgatctgcg ccgccagtgc ggagctctcc gtctgcagcg catcgatttc cgctgcgtga
     1561 ttcgcattgt cggctcgcac cagcgcgagt gtgcgctccc tggcgtcgtt cgccgcgacg
     1621 agctcgttcc gagttgcgac caagcgatct cggacagcgc gctgctcggc gacctgggcg
     1681 gcgaccgcca gcgtcgtcgc gcgcacttct acccgggttt cggcggtctc ctggcggacc
     1741 ttcttcgtct tctgttcccc atctgcgacg cgggcggcga tgcgtgcgtc ctggcgaccg
     1801 atgtcgttca gaagttcgat gttgtcgagg aggtccccga acgagacagc gcccagcacg
     1861 aaggagagaa catcgggagt gtcctccatg tagatctcac ggacacgtct ctccaaatgt
     1921 gcgacggcgg tgacatgctc ggcctcgaac gctctcagct gcgccgtctg gctctcgaag
     1981 cgctcgttga ggcgggcgag cttgccgcgc tcagaggcca gctgcgcttc gagcccgtag
     2041 agccgctgct gctcggcatc gacctgcccc tgcacttcgc ggacgcgtga gctggcggcg
     2101 gtgatctcgg aggtgagcac cgcctcctct tgctccgcc
//
LOCUS       DBNGNK010000322         1550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_916620,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000322 DBNGNK010000000
VERSION     DBNGNK010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_916620"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..491
                     /locus_tag="AC150V_09520"
     CDS             120..491
                     /locus_tag="AC150V_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763195.1"
                     /translation="MTTTASRNVRIEIAELATEAARSLDDVVEVRGGLGHSTSSAGER
                     VDGARCLAGRDGRYDVSVQVVARAVPLPALAERLRRRLSDACAERDLGDLLGQINVAV
                     ADVLLGGGGSAEGKPDPPLAG"
     gene            488..1084
                     /locus_tag="AC150V_09525"
     CDS             488..1084
                     /locus_tag="AC150V_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763196.1"
                     /translation="MSGSIVAETVTRVVAFIALVALALLGLTAAVFSIQAPGQPLSLG
                     ELAVHLELPALYQVVDDWTGRLEESGGLAGWSALGCSIAMLAGTALIGAALFPRRARF
                     VVVTDDEEGTLRYERQAVADLLAAEARRVSGVVDASVDVHARRWQTGGRAAVAVAAAH
                     GANRDQLATRVNGQLAPMAASLQLRLRTRCKGKTGWAG"
     gene            1081..>1550
                     /locus_tag="AC150V_09530"
     CDS             1081..>1550
                     /locus_tag="AC150V_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763197.1"
                     /translation="MIFRRLPRVESSALAGTGRLVALLIGLVVLWYGLAGLLLAFKVD
                     PDAVDAISGYRLAVDAAGALRAEDASALARALGAGLGLFVFASALFLAWALVPRRVRG
                     RRELPLDGGADGRLHLRPRAVERVAETATRRALGGAETDGRYEGDRLTIRVAITR"
BASE COUNT          214 a          508 c          571 g          257 t
ORIGIN      
        1 aacatccgcg aacgcgttat cagcaacgtg cacaacctga ccgggctccg cgtgacggag
       61 gtcgacattc aggtcgacga cgtccacgtg cccgaccagg acagcagcaa cgggccgcag
      121 tgaccacaac ggcctctcgc aacgttcgaa tcgagatcgc ggagctcgcg acggaagcgg
      181 cacggtcgct cgacgacgtg gtcgaggtgc ggggtgggct cgggcactcg acatcgagcg
      241 ccggcgaacg ggtggacgga gcccgatgtc tggccggtcg tgacggccgc tacgacgttt
      301 cggtgcaggt ggtcgcgaga gcggtgccgc tgccggcgct cgcagagcgc ctgcggcgcc
      361 ggctgtccga tgcgtgcgcc gagcgggatc tcggcgacct gctcggccag atcaacgttg
      421 ccgtggcgga cgttctgttg ggagggggcg ggtcggctga ggggaagccc gacccgcctc
      481 tcgccggatg agcggaagta tcgttgccga gacggttacg cgagtggtcg cgttcattgc
      541 gctcgtcgcg ctcgcgctgc tcgggctcac tgcggcggtc ttctcgatcc aagcgccggg
      601 ccagcctctg tcgctcggcg aactggccgt gcatctcgag cttcccgcgc tttatcaagt
      661 tgtcgacgac tggacgggtc ggctcgagga gtcgggtgga ctggcgggct ggtcggcgct
      721 cggctgcagc atcgcgatgc tcgcgggtac cgctctaata ggcgcagcgc tgtttccgcg
      781 acgagcccga ttcgtggtcg tcacagacga cgaggaggga acgctccgct acgagcgtca
      841 agccgtggca gacctgcttg ccgcggaggc tcggagagtg tccggcgtcg tggacgcgtc
      901 tgtggacgtc catgcccgcc gctggcaaac gggagggcgg gccgcggtcg ccgtcgccgc
      961 cgcgcacgga gccaaccgtg accaactggc cacccgtgtg aatggccagc ttgctccgat
     1021 ggcggcctca ctccagcttc gcctccggac gcgctgcaag ggcaagactg ggtgggcagg
     1081 atgatcttca ggcggctccc gcgagtcgag tcgtcggcgc tcgccgggac cgggcgcctc
     1141 gtcgcactgc tgatcgggct cgtcgtcctc tggtacgggc tcgccggcct tctactggct
     1201 ttcaaagtcg accccgacgc cgtcgatgcg atcagcgggt accgactcgc ggtcgacgcg
     1261 gccggtgctc tgcgtgccga ggacgcgtct gcgctcgcac gcgcccttgg agcagggctc
     1321 ggtctctttg tcttcgcgag cgcgctcttc ctggcatggg cgctcgtccc gcgccgggtt
     1381 cgcgggcgtc gtgagttgcc tctcgacggc ggggcggacg ggaggctgca ccttcgacct
     1441 cgcgcggtcg agcgcgtggc cgaaaccgca acgcgtcgcg cgctcggcgg tgccgagacc
     1501 gacggcaggt acgaggggga ccgcctgacg attcgggtcg cgatcacacg
//
LOCUS       DBNGNK010000323         4150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_272273,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000323 DBNGNK010000000
VERSION     DBNGNK010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_272273"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC150V_09535"
     CDS             <1..308
                     /locus_tag="AC150V_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HIR3763198.1"
                     /translation="HLWRAAPACSLVHDVAEGGLAIALAEAAFYSGIGAELDLPIDAT
                     VLFGEGGGQAIVALPPGRVEVDPTGSDVELRRIGVVGGETLLGVDVSELRAAWTAEA"
     gene            312..1694
                     /gene="purF"
                     /locus_tag="AC150V_09540"
     CDS             312..1694
                     /gene="purF"
                     /locus_tag="AC150V_09540"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR3763199.1"
                     /translation="MCGVFGIRSTERDVARLAYFGLFALQHRGQESAGIAVSEQGRLT
                     VLREMGLVAQVFDEDKLHALPGDVAIGHTRYSTTGSSHWDNAQPLVRHGAARTVAVGH
                     NGNLVNTAELRQELVAGGAALASTSDTEVIAALIAADPLPLPEAVAVAMARLEGAYSA
                     TVLAEHTLVAFRDPHGIRPLVLGRLGDDWVVASESCALDLIGAEAERDVRPGEVIWVD
                     AEGLHTLQALPSGRDAQCIFEHVYFARPDSKVGGVAVHAARVRMGERLAEEGPVEADL
                     VMPIPDSGTPAAIGFAKASGIPYEEGLIKNRYVGRTFIEPDQELRRQGIRLKFNPLEE
                     IAGRRIVIVDDSIVRGNTMRQLISMLFDARAEEVHVRVSSPPVVSPCYYGIDMADEAQ
                     LAAAHRSVEEMREHIGATSLHYLSVAGMQAATGLPEDAVCRACFTREYPTLVPAERNL
                     AKLRFEPASL"
     gene            complement(1691..2554)
                     /locus_tag="AC150V_09545"
     CDS             complement(1691..2554)
                     /locus_tag="AC150V_09545"
                     /inference="COORDINATES: protein motif:HMM:NF021214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR3763200.1"
                     /translation="MAATSLLTSWTLDPLQIAPIALIAAAYTLRVRTLGRRGQPVAGW
                     RIALFSLGIALLLGAVVSPVNAVGEEELVSFHMLQHILIGDLAPLCLLAGLTGPILQP
                     LLAVLPVNKLRVLAHPLVALPIWALNLYIWHIPFLYEAAVEYSLVHTIEHVSFFTAGI
                     VMWMPVLETLPAPEWFGTGAKLAYIVAVRMVETLLANIFLWSGDPFYSVYAQSGTFIG
                     ISPTADQALAGGILLLSGSLVTATAIAWLFLRMAQESEIRQQLLERGLDPRTVRRAVR
                     YGRWRELADES"
     gene            2586..3545
                     /gene="purM"
                     /locus_tag="AC150V_09550"
     CDS             2586..3545
                     /gene="purM"
                     /locus_tag="AC150V_09550"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR3763201.1"
                     /translation="MPSEEQTYAAAGVSLARADAVVERLRAAVESTRTPGVHGSFGAF
                     AGLYEIDDRRLLAASTDSVGSKLVLARRARRLRWCGMDLAAHCVNDVLTTGAEPLFFL
                     DYVAANRIDPEQVAELVEGAADVCRSAGCAIVGGETAELPGIYRAGELDFCGTAVGVV
                     DRERLVDGSRVAAGDCVIGFYSAGIHANGFSLVRSIVGDSDFDADLVLAPTRLYLDDV
                     RALRARADVRALAHVTGGGIVSNLGRALPKGLGAVIDPAAWRRLPVFDWLLSEGVDED
                     ELRRVFNIGIGYCAVVPETDVIDSDLLIGRVGETVRGVVWTDR"
     gene            3542..4084
                     /gene="purN"
                     /locus_tag="AC150V_09555"
     CDS             3542..4084
                     /gene="purN"
                     /locus_tag="AC150V_09555"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR3763202.1"
                     /translation="MIGVLVSGSGSNLQALLDAGLPVVAVASNVAGARGLERAAGAGV
                     DTNVFALADYADRDARDEAMADWLVDHDVSLVVCAGYMHLLRPCFLERFPDRVLNVHP
                     ALLPAFPGAHAVEDALAAGVGETGATVHLVDEGVDTGKVLLQEAVPVLAGDTADALHD
                     RIRAVEHRILPQVVRELVGQ"
BASE COUNT          615 a         1384 c         1496 g          655 t
ORIGIN      
        1 gccatctgtg gcgggccgcg ccggcctgct cgctggtgca cgacgtcgcc gaaggtggac
       61 tcgcgatcgc gctcgcagag gcggccttct actcggggat cggagccgag ctcgacctgc
      121 cgatcgatgc gaccgtcctc ttcggcgaag gcggcggcca ggcgatagtc gccttgccgc
      181 cgggccgggt cgaggtcgac ccgaccggca gcgatgtaga gctgcgccgc atcggcgtcg
      241 tcggcggaga gacgctcctc ggcgtcgacg tgtccgagct gcgggctgcc tggacggcgg
      301 aggcctaacc gatgtgcggc gtcttcggca tcaggtcgac ggagcgcgac gtggcacggc
      361 tcgcatactt cggcctcttc gccctgcagc accgcggcca ggagtccgcg ggcattgcgg
      421 tgtccgagca ggggcggctc accgtgctcc gtgagatggg gctggttgcg caggtgttcg
      481 acgaagacaa gctccacgcg ctgccgggcg acgtggcgat cggccacacg cgctactcga
      541 cgacgggctc ttcgcactgg gataacgcgc agccgctcgt tcgtcacggc gccgcccgca
      601 cggtcgcagt cggccacaac ggaaacctcg tcaatacggc cgaattgcga caggagctgg
      661 tggcgggcgg tgcggccctc gcgtcgacgt cggacactga ggtcatcgcc gccttgatcg
      721 cggccgatcc gctgcctcta cccgaggccg tggccgtcgc catggcgcgc ctcgaaggtg
      781 cctactccgc aacggttctc gcggagcaca ccctggtcgc gttccgcgat cctcacggca
      841 ttcgccccct cgtgctcggt cggctgggcg acgactgggt cgtggcgtcc gaaagctgcg
      901 ctctcgatct gatcggcgcg gaggcggagc gcgacgttcg ccccggcgag gttatctggg
      961 tcgacgctga agggctccac accctgcagg ccctgccctc ggggcgggat gctcagtgca
     1021 tcttcgagca cgtgtacttc gcccgaccgg actccaaggt cggcggtgtc gcggtgcacg
     1081 ccgctcgggt gcggatggga gagcggcttg ccgaggaggg gccggtcgag gccgatctcg
     1141 tgatgccgat ccccgactcc ggcacgccgg cggcgatcgg cttcgccaag gcgtcgggca
     1201 tcccttacga ggagggcctg atcaagaatc ggtatgtggg aaggacgttc atcgagcccg
     1261 accaggagct gcgccggcag gggatccggc tgaaattcaa ccccctcgag gagatcgcgg
     1321 gtcgccggat cgtgatcgtc gacgactcga tcgtgcgcgg caacacgatg cggcagctca
     1381 tctcgatgct cttcgacgcc cgcgccgagg aggtgcacgt tcgggtctcg tcgccgcccg
     1441 tcgtgtcgcc ctgctactac gggatcgaca tggcggacga ggcgcagctg gcggccgccc
     1501 accgctcggt cgaggagatg cgcgagcaca tcggcgcgac gtcgctccac tatctgtcgg
     1561 tcgcggggat gcaggccgcg accggattgc ccgaggatgc cgtctgccgg gcgtgcttca
     1621 cgcgcgagta cccgaccctc gtgcccgcag agcggaacct ggcaaaactc cgcttcgagc
     1681 cggcgagcct ctagctctcg tccgcgagct cccgccagcg gccgtagcga accgcgcgcc
     1741 ggaccgtgcg cgggtcgagt ccgcgctcga gcagctgctg gcggatctcg ctctcctggg
     1801 ccatccgcag gaagagccag gcgatcgcgg tcgcggttac gagcgagccg ctcagtagca
     1861 ggatgccgcc cgcgagtgcc tgatccgcgg tcggcgagat cccgatgaag gtgcccgact
     1921 gcgcatagac ggagtagaag gggtcaccag accagaggaa gatgttcgcg agcaacgtct
     1981 cgaccattcg gacagcgacg atgtacgcga gcttggcgcc ggtgccgaac cactccggtg
     2041 ccggcagcgt ctccagcacc ggcatccaca tcacgatccc tgccgtgaag aacgagacgt
     2101 gctcgatcgt atgcacgagc gagtactcga ccgcagcctc gtagaggaac ggtatgtgcc
     2161 agatatagag gttcagagcc cagatgggca gcgcgacgag tgggtgtgcc agtacgcgga
     2221 gcttgttcac gggaaggacg gcgaggagcg gctgcaggat cgggcccgtc aggcccgcga
     2281 ggagacagag cggggcgagg tcgccgatca ggatgtgctg cagcatgtgg aagctcacca
     2341 gctcctcctc gccgacggcg ttcacggggg agaccacggc gcccagaagg agggcgatgc
     2401 cgagcgagaa gagggcgatg cgccagccgg cgacgggctg accgcggcgc ccgagcgtcc
     2461 gcacccgcag cgtgtacgcg gccgcgatca gtgcgatcgg cgcgatctgc agcggatcga
     2521 gcgtccagct ggtcaggagc gacgtggcgg ccactcggtc actgtaggcc gcgtagactc
     2581 gacccgtgcc ctccgaggag cagacgtacg ccgctgcagg cgtgtcgctt gccagggcgg
     2641 atgccgtcgt cgagcgtctc cgggcggcgg tcgaatcgac ccggacaccg ggtgtgcacg
     2701 gctcgttcgg ggccttcgcc ggcctgtacg agatcgacga ccgtcgcctg ctcgccgcct
     2761 cgaccgactc ggtgggctcg aagctcgtgc tggcacgacg ggcaagacgg cttcgctggt
     2821 gcgggatgga tctggcagcc cactgcgtca acgacgtgct caccacaggt gccgagccgc
     2881 tcttcttcct cgactacgtg gccgcgaacc ggatcgatcc ggagcaggtc gccgagctcg
     2941 tggaaggggc cgccgacgtc tgccgctcgg ccggctgcgc gatcgtgggc ggagagaccg
     3001 cagagctgcc cggcatctac agggccggcg agctcgactt ctgcggcacc gccgtcggtg
     3061 tcgtcgatcg cgaacggctc gtcgacggct ctcgagttgc cgccggcgat tgtgtgatcg
     3121 gcttctactc cgctggtatc cacgccaacg gcttctcgct ggtgcgcagc atcgtcggcg
     3181 actccgactt cgacgccgat ctggttctcg cgccgacgcg cctctacctc gacgacgttc
     3241 gggcactccg ggcacgcgcc gacgttcggg ccctcgcgca cgtgaccggg ggaggcatcg
     3301 tcagcaacct cggacgcgcg ctgccgaagg gcctcggcgc ggtgatcgac ccggccgcct
     3361 ggcgacgcct tcccgtcttc gactggctgc tgtccgaagg agtcgacgag gacgagctgc
     3421 gccgggtctt caacatcggg atcggttact gcgcggtcgt gccggagacg gacgtcatcg
     3481 attcggatct cctcatcggg cgtgtcggcg agacggttcg gggcgtcgtg tggacggatc
     3541 ggtgatcggc gttctcgtca gcggcagcgg ctccaacctg caggcgttgc tcgatgccgg
     3601 gctgcccgtg gtggcggtcg cgtcgaacgt cgcgggggcc cgggggttgg agcgggcggc
     3661 cggggccggg gtcgacacga acgtcttcgc gctcgccgac tacgccgatc gagacgctcg
     3721 cgacgaggcg atggccgact ggctcgtcga ccacgacgtc agtctggtcg tgtgcgccgg
     3781 ctacatgcat ctgctgcggc cgtgcttcct ggagcgcttt cccgatcgcg tgctgaacgt
     3841 ccatcccgcg ctgctgccgg cttttccggg ggcccatgca gtcgaggacg cgctggcagc
     3901 aggggtgggc gagaccggcg ccaccgtgca cctcgtggac gaaggcgtcg acacgggaaa
     3961 ggtcctgctc caagaggccg tgccggtgct ggcgggcgac accgccgatg cactgcacga
     4021 ccgcattcgc gcggtcgagc accgcatcct ccctcaggtg gtgcgggagc tggtcgggca
     4081 atgaaagtcg agcgcgcgct cctctccgtc tacgacaagg acggcctggc cgccttcgcg
     4141 cagggtctgc
//
LOCUS       DBNGNK010000324         6711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_629268,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000324 DBNGNK010000000
VERSION     DBNGNK010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6711
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_629268"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..427
                     /locus_tag="AC150V_09560"
     CDS             <1..427
                     /locus_tag="AC150V_09560"
                     /inference="COORDINATES: protein motif:HMM:NF026109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3763203.1"
                     /translation="ARFDDPVLGASRRIEHWDNASYHGDLLGRGLAGEADAAYDHVSM
                     FFSDVFDFSFELYGDIAQHERTVTRGSFEEHDAVAAYLDANHRLVAALAVGQSEETTQ
                     TLEELIAARAALTEPAVLRDDSAAVDGAFSVPTRSGVGR"
     gene            547..804
                     /locus_tag="AC150V_09565"
     CDS             547..804
                     /locus_tag="AC150V_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009801022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7218 family protein"
                     /protein_id="HIR3763204.1"
                     /translation="MAKDHGPSIKDDERYERLREQGMGKEKAARIANTPRREAGRKGG
                     SAEPYEEWTKEELYDRAREIGIDGRSKLSKSGLINALRNGR"
     gene            922..1386
                     /locus_tag="AC150V_09570"
     CDS             922..1386
                     /locus_tag="AC150V_09570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR3763205.1"
                     /translation="MAIGTGDRAPEFDFEETYERRVRLSDFLGRSNVLLVFHPFAFTP
                     VCAEEAIDLQENIASFRHAETDIVFVSCDTSAARQAWRRELGAEYTFASDFWAHGANA
                     KAYGVFDESSGAAVRGTFLIDKDGVVIWSLVKDADSRRTEMVPESLEKLDDQ"
     gene            1427..2218
                     /locus_tag="AC150V_09575"
     CDS             1427..2218
                     /locus_tag="AC150V_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3763206.1"
                     /translation="MRELAVRRWGNEKAARVVFLHGVTSHGNHARLLAENWLAERYCV
                     IAPDLLGHGDSPYEPPWDLDTHVDAVLASVGSEPAAWIGHSFGARLAVEIAARERAAV
                     ERLVLLDPALLLPPHVALFAAEHARAERAYASFEEGIDRRFDESSLVSTPREVLKTEL
                     ADHLVRRGDGRWGYRYSQASVVAAYGEMASAPPSFDRVRMPTLLVLGERSYVPYDHLL
                     AAHRDALGDLLEVVLVPGGHTVLWDSLAETAAAVTAFLAASDVNG"
     gene            complement(2222..3574)
                     /locus_tag="AC150V_09580"
     CDS             complement(2222..3574)
                     /locus_tag="AC150V_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3763207.1"
                     /translation="MTTVETNPNLGAEIVADAKEFVLYSWSVPNQNQPIAVSGAEGRH
                     FWDYEGKRYLDFASQLVNVNIGHQHPKIIEAIKEQADRLCTIGPPMANESRSRLGRLL
                     AEVTPGDLSVSFFTNGGAEANENAIKLARLVTGRHKIVARYRSYHGATHGAVTLTGDP
                     RRWAVEPGLPGIVRAFDPYCYRCPCGQESACRVCTGAPHLEEILEYEGAPTVAAVVLE
                     TVTGTNGIIVPPDGYLQSVREVCDRHGILLILDEVMAGFGRTGKWFACDHWDVVPDVI
                     TVAKGINSGYVPIGAMIARGPIVDQIRDRFFPGGLTYAGHPLACASAVASIEAFREEG
                     VVENAAAIGDYFAGALAGLRERHPSIGDVRGLGCFWGLELVKNRDTREMLVPYNASGE
                     AAAPVARLAKAALERGLYLMTHWNVIMIVPPLTITLEEAEEGVAILDDVLAIADEYVA
                     "
     gene            3673..4920
                     /locus_tag="AC150V_09585"
     CDS             3673..4920
                     /locus_tag="AC150V_09585"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019008470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/carbamoylase family amidase"
                     /protein_id="HIR3763208.1"
                     /translation="MALDPSRVVAQLKELRELTGDDDGAQRVAWTDTWAKARAWLAEQ
                     LSDLPLEVERDQAANDWYTLRGESDRAVLIGGHMDSVPNGGWLDGSLNVLAGAEVLRR
                     IAGDGTPAVTVRLVDWADEEGARFGRSLIGSSAASGSMSDQDEMRKMSDRDGVSLPDA
                     LARFDVDLDSALEAHAQLASAAAYLELHIEQGPVLESMDLPLGAVLGTFGVERHKIIW
                     RGQAAHSGSTPMEQRRDALAGASKLALAIRDIAREAGGGAVCTSGDVVCRPGIVTSVV
                     ETAEQLLDQRHLDAETLAWMLSRAKELSARFAEEENIEVQWERLWNIEPLLFDETLVG
                     FCDEAITEVAGTSHKLPSGPLHDAAEVAKAGVPTVMMFAQSLRGLSHTKLEDTREEHI
                     ELSVAAMDGLAARTIDWVASGDG"
     gene            4949..6037
                     /locus_tag="AC150V_09590"
     CDS             4949..6037
                     /locus_tag="AC150V_09590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3763209.1"
                     /translation="MLGASVTWDVSNTGAVADTLATTYGVSLAVVGVLTTALFLTHLL
                     VQLPGGRLIDRLGARRIGLASLAIVAGGNALCLVGPSFELALAGRTIMGLGTGAGFVA
                     GVDLVRTGGGGAFGQGAYGGATMAGGGLAIMVVPALVGPLGWRAPYWTGLALALVAVG
                     PVVAGGPDAVTRHEGVRVAVLRDTRLLPLGAIQAATFGLAVVAGNWVVALLERLDVGR
                     GLAGAVGGLVLFAGIVTRPLGGWIVAHRPHASRPLVAASLVAGGLAVLVLATGPPVPV
                     AAVAALAAGLAAGLPFAAVFAATQRLRPDAPATAVGLVNGCAILAIVVLTPLAGLAFS
                     LPGEGRLAFGAIGVLWLLSLVALRRTPL"
     gene            6098..>6711
                     /locus_tag="AC150V_09595"
     CDS             6098..>6711
                     /locus_tag="AC150V_09595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763210.1"
                     /translation="MSELSEFKERQRAMWATGDYHSVSELISDVGKRIVARVGIRPGD
                     DVLDVACGSGNATIPAAAAGARAVGVDLTPELFESASAAAAAAGVEVEWIQGDAEELP
                     FDDASFDVVLSTFGCMFAPRQRVTAGELDRVLRPEGRLGLCNWRPDGTVGEFFGVVAS
                     YVPPSPLYESPPTLWGTEDHVRALFADSPIDFEFERTHVEVEFES"
BASE COUNT          974 a         2281 c         2400 g         1056 t
ORIGIN      
        1 cgcccgcttc gacgatccgg tgctgggcgc gagccgccgt atcgagcact gggacaacgc
       61 gagctatcac ggtgatctgc tcggccgcgg gctcgcgggc gaagcggatg ccgcgtacga
      121 tcacgtctcg atgttcttct cggacgtctt cgacttctcg ttcgagttgt acggggacat
      181 cgcgcagcac gagcgcaccg tcacgcgcgg cagcttcgag gagcacgacg ccgtcgccgc
      241 ctatctcgac gcgaaccatc ggctggtcgc cgcactcgcg gtcggccagt ccgaggagac
      301 gacgcagacg ctggaagagc tcatcgcggc ccgcgcggcc ctgacagagc cagccgtcct
      361 tcgcgacgac tccgccgcgg tcgacggcgc cttcagcgtg ccgacgcgca gcggcgttgg
      421 ccgttgagcg gaaagccagg gccggttgct ctggggacgg gtgccaaacc gcctccggat
      481 gcggaggacg cacgtttcgg gtacgcggcc gggtaaaccc ccgcggaatc gagaggaggc
      541 agaagaatgg cgaaggatca cggtccgtcc atcaaggacg atgagagata cgagcggctg
      601 cgcgagcagg gaatgggcaa ggagaaggcc gccaggatcg ccaacacccc ccggcgcgag
      661 gcgggccgca agggtggttc ggccgagccg tacgaggagt ggacgaaaga ggagctctac
      721 gaccgcgcga gggaaatcgg catcgacggc cggtcgaagc tctcgaagtc cggattgatc
      781 aacgccctcc ggaacggccg ctaggcgcgc tggcgctgac tgctcgcgat cgtcgggcga
      841 ccggccctca gtaccagggc accggacagc ctgcggagac gacggtacta tcgcccctcg
      901 gaagccaacg agaggattca cgtggcgatc gggacgggtg acagggcgcc cgagttcgac
      961 ttcgaggaga cctacgagag gcgcgtgcgc ctgtccgact tcctcgggcg cagcaacgtg
     1021 ctgctcgtgt tccacccgtt cgccttcacg cccgtctgtg cggaagaggc gatcgatctg
     1081 caggagaaca tcgcttcttt caggcacgcc gagacggaca tcgtcttcgt ctcctgcgac
     1141 acgtcggcgg cacggcaggc ctggcgtcgc gagctcggtg ccgagtacac gttcgcgtcc
     1201 gacttctggg cgcacggggc gaacgcgaag gcatacggcg tcttcgacga gtcttccggc
     1261 gctgccgtac gcggcacctt cctgatcgac aaggacgggg tggtgatctg gtcgctcgtc
     1321 aaggatgcgg actcgcgacg cacggagatg gtgccggagt cgctcgagaa gctcgacgac
     1381 cagtgatcgt gctcggcgga gccacgccgc cgcccggaac gtcgacatga gggagctcgc
     1441 ggtgcgccgt tggggcaacg agaaggccgc gcgggtcgtc ttcctgcacg gggtcacgag
     1501 ccacggcaat cacgcccgcc tcctcgcgga gaactggctc gccgagcgct actgcgtgat
     1561 cgcacccgat ctgctcggcc acggcgactc gccgtacgag ccgccgtggg acctcgacac
     1621 gcacgtcgac gccgtgctgg catctgtcgg aagcgagccg gccgcctgga tcggtcactc
     1681 cttcggcgcc cgcctcgccg ttgagatcgc tgcccgcgag cgggctgccg tcgagcggct
     1741 ggttctgctc gacccggcgc tcctcttgcc gccgcatgtg gccctgttcg cagccgagca
     1801 cgcacgagcc gagagggcgt acgcgtcgtt cgaagaggga atcgaccggc ggttcgacga
     1861 gagctccctc gtgagcacac cgcgggaggt gctcaagacc gagctggcag accatctcgt
     1921 ccgccgcggc gacggacgct gggggtaccg ctacagccag gcgtccgtcg tcgccgccta
     1981 cggcgagatg gcaagcgcgc cgccgagctt cgaccgcgtg cgtatgccga cactgctggt
     2041 actcggagaa cgctcctacg tcccgtacga tcatctgctc gcagcacacc gtgacgcact
     2101 cggcgacctg ctcgaggtcg tcctcgttcc cggtgggcac acggtgctct gggacagcct
     2161 ggccgagacc gctgccgcag tgaccgcgtt tctagccgcg tccgacgtga acggctagcc
     2221 gttacgcgac gtactcgtcc gcgatcgcga ggacgtcgtc gaggatggca acgccctcct
     2281 cggcctcctc caacgtgatc gtgagcggcg ggacgatcat gatcacgttc cagtgcgtca
     2341 tcaggtagag gccgcgctcc agcgccgcct tcgccagtcg cgcgaccggg gccgccgcct
     2401 cgccggaggc gttgtagggc acgagcatct cgcgagtgtc gcgattcttg accagctcga
     2461 gaccccagaa gcagccgagg ccgcgtacgt cgccgatcga cggatgccgc tctcgcagcc
     2521 cggccagcgc gccggcgaag tagtcgccga tggccgccgc gttctcgacg actccctcct
     2581 cccggaaggc ctcgatcgag gccacggccg acgcgcaggc gagcgggtgt cccgcgtacg
     2641 tgagtccacc ggggaagaag cggtcgcgga tctgatcgac gatcgggccg cgcgcgatca
     2701 tcgccccgat cgggacgtag ccggagttga ttccctttgc gacggtgatg acgtcgggga
     2761 cgacgtccca gtggtcgcag gcgaaccact tgcccgtgcg tccgaaaccg gccatcacct
     2821 cgtcgaggat cagcagaatc ccgtgccggt cgcacacctc gcgcacggac tggaggtagc
     2881 cgtcgggcgg aacaatgatc ccgttggtgc cggtcaccgt ctccagcacg accgcggcga
     2941 ctgtgggtgc gccctcgtac tcgaggatct cctccaggtg gggcgcgccg gtgcagacac
     3001 ggcaggccga ctcctgcccg caggggcagc ggtagcagta cggatcgaag gcgcggacga
     3061 tcccgggaag accgggctcc accgcccagc gccgcggatc gccggtgagg gtgaccgcgc
     3121 cgtgcgtcgc gccgtggtac gagcggtagc gggcgacgat cttgtggcgt ccggtgacca
     3181 gccgggcgag cttgatcgcg ttctcgttcg cctcggcgcc gccgttcgtg aagaaggaga
     3241 cggacaggtc accgggtgtg acctcggcga gcaagcggcc gaggcgggaa cgggactcgt
     3301 tcgccatcgg cggtccaatc gtgcagagac ggtcggcctg ctccttgatc gcctcgatga
     3361 tcttcgggtg ctggtggccg atgttgacgt tgacgagctg cgaggcgaag tcgagatagc
     3421 gcttcccttc gtagtcccag aagtggcgcc cttcagcccc cgagaccgcg atcggctggt
     3481 tctggttggg aaccgaccac gagtagagga cgaactcctt ggcgtcggcg acgatctccg
     3541 cgccgaggtt cggattggtc tcgactgtcg tcatctccga tctccttcgc tcgctcgccc
     3601 gcgatcgtag cgacgatcgg ctgcgctgga tgcgctttga acaccccggc ccggccggat
     3661 aggctcgtcc cgatggctct cgatccgtca cgcgtcgtcg cgcagctcaa ggagctccgc
     3721 gagctgaccg gcgacgacga cggcgcccag cgcgtcgcct ggaccgacac atgggcgaag
     3781 gctcgcgcat ggcttgccga gcagctctcg gatcttccgc tggaggtcga gcgcgatcag
     3841 gcggcgaacg actggtacac gctccggggc gagtccgacc gcgccgtcct gatcggaggc
     3901 cacatggact cggtcccgaa cggcggctgg ctcgacggct cgctcaacgt cctcgccggc
     3961 gcagaggttc tgcgccggat cgcgggggac ggcacgccgg cggttacggt gcgcctcgtc
     4021 gactgggccg acgaggaagg cgcccgcttc ggccgatcgc tgatcggctc gtcggcggcg
     4081 tcgggctcga tgagcgatca ggacgagatg cggaagatga gcgatcgcga cggcgtttcg
     4141 cttcccgatg cactggcgcg tttcgacgtc gatctcgaca gcgcgctcga ggcgcatgca
     4201 cagctggcct cggcggccgc gtacctcgag ctgcacatcg agcaggggcc cgtgctcgag
     4261 tcgatggatc tccccctggg cgccgtgctc ggcacgttcg gcgtggagcg gcacaagatc
     4321 atctggcgtg gccaggcggc ccattcgggg tcgacgccga tggagcagcg gcgggacgca
     4381 ctggcgggcg cgtccaagct cgccctggcg atcagggaca tcgcgcgcga ggcgggaggc
     4441 ggtgcggtgt gcacgtcggg cgacgtcgtc tgcaggccgg gaatcgtcac ctccgtcgtc
     4501 gagacggccg agcagctact cgaccagcgc catctcgacg cggagaccct cgcctggatg
     4561 ctctcccggg cgaaggagct gtccgcgcgg ttcgccgagg aagagaacat cgaggtccag
     4621 tgggagcggc tctggaacat cgagcccctc ctcttcgacg aaacactcgt cggcttctgc
     4681 gacgaggcga tcacggaggt agcgggtacg tcccacaagc tcccgtccgg cccgctgcac
     4741 gacgcggctg aggttgcgaa ggccggcgtg ccgacggtga tgatgttcgc ccagagcctg
     4801 cgcgggctct cccacaccaa gctcgaggac acccgcgaag agcacatcga gctctccgtc
     4861 gcggcgatgg acggcctggc ggctcgcacg atcgactggg tcgcgtccgg cgacggctag
     4921 ccggccgccg aggatcgtcg ccgggctggt gctcggcgcg agcgtcacct gggacgtctc
     4981 gaacacgggc gcggtcgccg acaccctcgc gacgacgtac ggcgtctcgc tcgccgtcgt
     5041 cggggtgttg acgaccgcgc tcttcctgac ccacctcctc gtccagctgc cgggtggccg
     5101 gctgatcgac cgcctcggag cgcgtcgcat cggcctcgcg tcgctcgcga tcgtggcggg
     5161 cggcaacgct ctctgtctcg tcggcccctc cttcgagctc gccctggccg gtcggacgat
     5221 catgggcctc ggcacgggag cgggcttcgt cgccggcgtg gacctggtcc gtacgggcgg
     5281 cggcggagca ttcgggcagg gcgcctacgg cggtgcgacg atggccggcg gcggtctggc
     5341 gatcatggtc gtccctgcgc tcgtcggccc cctcggctgg cgggcgccgt actggacggg
     5401 tctggcgctc gcactcgtcg cggtcggtcc cgtcgtcgcg ggcggccctg atgccgtcac
     5461 caggcacgag ggagtccgcg tcgcggtgct tcgcgatacc cgcctgctgc ccctcggcgc
     5521 gatccaggcg gcgacgttcg gcctggccgt cgtcgccggc aactgggtcg tcgcgctgct
     5581 cgagcggctc gacgtggggc gcgggctcgc gggcgccgtc ggcggcctcg tgctctttgc
     5641 cggaatcgtc acgaggccgc tcggtggttg gatcgtcgcg catcgcccgc acgcttcccg
     5701 gccgctcgtc gccgcgagcc tggtcgccgg ggggctcgcg gtgctcgtgc tcgcgaccgg
     5761 gccgccggtt cccgtcgccg ccgtggcggc gctcgccgcc gggctcgccg ccggcctgcc
     5821 gttcgccgcg gtcttcgccg caacgcagcg tctcaggccc gacgcgccgg cgaccgccgt
     5881 cgggctcgtc aacggctgtg cgattctcgc catcgtcgtg ctgacgccgc tggccggcct
     5941 tgccttctcg ctcccgggcg aggggcgtct ggcgttcggc gcgatcgggg tgctctggct
     6001 cctctcgctg gtcgcccttc ggcggacgcc gctctgagca ggcgttgtct cgacgcagcc
     6061 gcagcgctac ggtcctcgaa tccggaggga aggagccatg agcgagctat ccgagttcaa
     6121 ggaacgacag cgtgcgatgt gggcgacggg tgactaccac agcgtctccg agctgatctc
     6181 cgacgtcggg aaaaggatcg tcgctcgcgt cggcatccgg ccgggtgacg acgtcctcga
     6241 cgtcgcgtgc gggagcggta acgccaccat cccggccgcc gccgccgggg cgagagcggt
     6301 cggggtcgat ctgacgccgg agctgttcga gagcgcaagc gccgcggcgg cggcggccgg
     6361 agtggaggtc gagtggattc agggcgatgc ggaggagctc ccgttcgacg acgcgagctt
     6421 cgacgtggtt ctctccacct tcgggtgcat gttcgcgccc cggcagcgag tgaccgctgg
     6481 cgagctggat cgggtgctgc gtccggaagg gagactgggg ctgtgcaact ggcggccgga
     6541 cggcacggtc ggtgagttct tcggcgtggt cgcaagctac gtccccccct cgccgctgta
     6601 cgagtcgccg cccacgctct ggggaacgga ggatcacgtg cgtgccctct tcgccgacag
     6661 cccgatcgac ttcgagttcg aacgcactca cgtcgaggtc gagttcgagt c
//
LOCUS       DBNGNK010000325         4902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1131961,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000325 DBNGNK010000000
VERSION     DBNGNK010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4902
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1131961"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="AC150V_09600"
     CDS             complement(<1..1124)
                     /locus_tag="AC150V_09600"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR3763211.1"
                     /translation="MNQPELLYRRHEFLARAGRLVAAAAGGALLSDSAARAARTPADP
                     IAATAVRAAKRFAGTKLEFFPIWGWERADEEVSFAAQWTELTGVEVELVPPPAKDVFE
                     VDVRQIPDWAEKGWMEPLDRFIAKYRAAAGMPDYHPLTRSLAVYRGKAWGFVDDSDVW
                     PVFYRKDLFQDPRLRSTYRSIFRRDLRVPRTWEEFARTAQFITDQLSPAVYGTGMGRE
                     LVPDVEYASHLYFYFHFQRHGGRFFDPRTMRATINRPAGARAVRQMLNENDASIPGIE
                     KLDFVDIYTLWLQGRIAMLTTWPVLSRVSENVGRRGDALRFMPRSKVAGKVGYTVVGG
                     SALTTGGFAQAVARTSKHKEAAYLFAQWLTSPSISLARLEV"
     gene            complement(1121..3037)
                     /locus_tag="AC150V_09605"
     CDS             complement(1121..3037)
                     /locus_tag="AC150V_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR3763212.1"
                     /translation="MLRSPATEEGRLLLSSDLRPGSEFVGYRIEALLGRGGMSVVYRA
                     EDLALERKVALKLLAAQLAEDERFRARFQRESRLAASIDHPHIVPVYEAGEAQGQLFI
                     AMRYVEGTDLTKLLRDEGALAPERTLSLLDQLAGALDTAHERGLIHRDIKPSNVLLTR
                     QAGTEHCYLGDFGLTMSASERSGPPKEGQIEGTVDYLAPEVIEGEPADESSDLYALGC
                     LLYQSLTGEAPFRRDSELATLWAHVREEPPKLSERDPELPPELDEVIAKALAKDPAER
                     YATAGELQAAAAAALALDEPETSRRRWLIAGVAVLLAAALAAVLAVVFTGSDSGPSTT
                     PTLDITTGSIQHLDPQTNELVATIEAPGFSVIEAGGGSVWAANADANRIVRIDPETNR
                     VGQAIDGIVNPEVLAFGAGALWALNPVGGILTRIDPASGSVTRSISLPPGLLSAGIGG
                     LMRATEHNIWVSWITRRGNAARIDAQSGAVDIVQIGPDRSDFTIDFVPVDDSTLWVLA
                     PTAEGAAVSLLRVDPADPARVVTAVPGLHFGNAVFDGESIWVSLPFLGNVVARIEPRT
                     GRIRDRVQAGAFPGKIAVGGDALWVANWDEGTLARVDLATRTVVETIPIGPNPHAIAV
                     TEKGIWVAVRPDDL"
     gene            complement(3057..4607)
                     /locus_tag="AC150V_09610"
     CDS             complement(3057..4607)
                     /locus_tag="AC150V_09610"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR3763213.1"
                     /translation="MEFRILGPLEVLDDDGPVGIRGARLRALLAVLLLHANQFLSSDR
                     LLDEVWGEEPPRSGAIALRMRVAELRKKLGADRLETRSPGYALRVASDELDLWRFERL
                     VDAAAEAEPAEALALLDDALALWRGPALANVEHESFAEASVARLEELRLVARERRAAA
                     LVDVGRPEEAVEELRALVAEHPYREQLRADLMRALYKAGRQAEALAAFQDTRQMLVEE
                     LGIDPGPALQELERAILRQDPALAAETPTAGPARTVLIVSSTERGLDTLLALSTPLAS
                     GDGRELMLVRTVAAAELGDESERLASRRERLIASGAAARTAAFASQAPGRDLARLASE
                     QDADLILHEGELTPDVVALLEAASSDVALLPEVRAGGPLGPDRPVLVPFTGADHDWAG
                     VELAAWLARASDAPLVLAGRASEGEDASRLLASASLIVQRAVGINTSPLLVEPNAEGL
                     TAAAEGMGLVVFGLPPDWRRRGVGEVRLAVAQGTSVPTLLVRKGLRPGGLAPASSHTR
                     FTWTVQDA"
     gene            4690..>4902
                     /locus_tag="AC150V_09615"
     CDS             4690..>4902
                     /locus_tag="AC150V_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763214.1"
                     /translation="MENASRKEHTTGAATRFARRSVGPGGQAGRLVRASIAAAAVVLL
                     ALGAGCGGDRDHGAERLIEESTVRTAG"
BASE COUNT          737 a         1686 c         1720 g          759 t
ORIGIN      
        1 acctcgaggc gggcaagtga gatcgacggg ctcgtcagcc attgggcgaa gaggtacgcg
       61 gcctccttgt gcttggacgt gcgggcgacg gcctgcgcga aaccgcccgt ggtgagcgcg
      121 gagcccccga cgacggtgta gccgaccttg ccggcgacct tggatctcgg catgaaccgc
      181 agcgcgtcac ctcgtcggcc cacgttctcc gagacgcgac tgaggacggg ccaggtcgtg
      241 agcatcgcga tgcggccctg cagccagagc gtgtagatgt cgacgaagtc gagcttttcg
      301 atgcccggaa tcgatgcatc gttctcgttc agcatctgtc tgacggcccg cgcgcccgcc
      361 gggcggttga tcgtcgcccg catcgttctg gggtcgaaga agcgtccgcc gtgtcgttgg
      421 aagtggaagt agaagtagag atggcttgcg tattcgacgt cggggaccag ctcgcgcccc
      481 attcccgttc catagacggc cggagagagc tggtcggtga tgaactgcgc cgtccgggcg
      541 aattcctccc aggtccgcgg cacgcgcagg tcgcgcctga agatcgagcg gtacgtgcta
      601 cgaagccgag gatcctggaa caagtccttc cggtagaaga cgggccagac gtccgagtcg
      661 tcgacgaagc cccacgcctt gccccggtac accgcgaggg atcgcgtaag tgggtggtag
      721 tccggcattc ccgccgcggc ccgatacttg gcgatgaacc ggtcgagtgg ctccatccat
      781 cccttttccg cccaatcggg gatctggcgc acatcgactt cgaagacgtc cttggccggc
      841 gggggaacga gctcgacctc gactccggtc agctccgtcc actgcgccgc gaagctcacc
      901 tcttcgtccg cccgttccca gccccagatc gggaagaact ccagcttggt gcctgcgaac
      961 cgcttggccg cgcgaacggc agtggccgcg atcggatcgg ccggcgtcct tgccgcgcgg
     1021 gcggcgctgt ctgagagcaa tgcccctcct gcggccgctg cgacgagtct ccccgcccgg
     1081 gcgaggaact cgtgtcgccg atagaggagc tcaggctggt tcacaggtcg tccgggcgaa
     1141 cggcgaccca gatgcccttc tcggtgaccg cgatcgcgtg tggattcggg ccgattggaa
     1201 tcgtctcgac gacggtgcgc gtggcgaggt cgacacgcgc aagcgtgccc tcatcccagt
     1261 tcgcgaccca cagggcgtcg ccgccgacag cgatcttgcc ggggaaggcc cctgcctgaa
     1321 cgcgatcccg gatccgacca gtgcgcggct cgatccgggc aacgacattt cccaggaacg
     1381 gcaggctgac ccagatgctc tcgccgtcga agacggcatt cccgaagtgg aggccaggga
     1441 cagccgtgac gaccctggcc gggtcggccg gatccaccct gagcagcgag acggcagctc
     1501 cctcagctgt gggtgccagc acccacagcg tcgagtcgtc gaccggtacg aagtcgatcg
     1561 tgaagtctga cctgtcgggg ccgatctgaa cgatgtccac ggctccgctc tgcgcgtcga
     1621 tacgagcggc gtttccccgc cgcgttatcc aggacaccca gatattgtgc tcggttgcac
     1681 gcatgagccc gccgattccg gcgctcagta gtcctggcgg cagcgagata gagcgcgtca
     1741 cgctccccga cgcggggtcg attcgcgtga ggatgccgcc gaccgggttg agtgcccaga
     1801 gcgcgccggc gccgaaggcc aggacttcag ggttcacgat gccgtcgatg gcctgcccga
     1861 cccggttggt ttccgggtcg atccgcacga tccggttcgc gtccgcgttt gcggcccaga
     1921 ccgagccgcc gccagcctcg atcacggaga atccaggcgc ctcgatcgtc gcgacgagct
     1981 cgttggtctg cgggtcgagg tgctggatgg agccggtggt gatgtccagg gtcggcgttg
     2041 tgctcgggcc ggagtcgctg ccggtgaaga cgactgcgag caccgcggcg agggcggccg
     2101 cgaggaggac cgcgacaccg gcgatcagcc agcggcggcg ggaggtctcg ggctcgtcca
     2161 gagcgagcgc ggccgccgct gccgcctgca gctcgcccgc ggtcgcgtag cgttcggcgg
     2221 gatccttcgc cagggccttc gcgatcacct cgtcgagctc gggcggcaac tcggggtcgc
     2281 gctccgacag ctttggcggc tcctcccgga cgtgcgccca gagcgtggcc agctccgagt
     2341 cgcggcggaa gggagcctcc cccgtcagtg actggtagag gaggcagccg agcgcgtaga
     2401 ggtcagagct ctcatcggcg ggctctccct cgatcacctc gggcgcgagg tagtcgactg
     2461 tgccctcgat ctgaccctct ttcggcgggc cgctccgctc ggacgccgac atcgtcaggc
     2521 cgaagtcgcc gaggtagcag tgctccgttc ccgcctggcg ggtgaggagg acgttggacg
     2581 gcttgatgtc gcggtggatc aggccgcgct catgtgcggt gtcgagagcg ccggcgagct
     2641 ggtccaatag cgagagcgtc cgctccggcg caagcgctcc ttcgtcacgg aggagcttcg
     2701 tcaggtcggt gccctccacg taccgcatcg cgatgaagag ctgcccctgc gcctcgcccg
     2761 cctcgtagac ggggacgatg tgcgggtggt cgatcgaggc ggccagccgc gactcgcgct
     2821 ggaaccgcgc ccggaagcgc tcgtcttcgg cgagctgcgc cgccagcagc ttcagcgcaa
     2881 cctttcgctc caaggcgagg tcttccgctc ggtagacgac gctcatcccg ccgcgtccga
     2941 gcagagcctc gatccggtat ccgacgaact cgctgcctgg gcgcaggtcg gacgaaagga
     3001 gcagacgacc ctcctctgtt gcaggagagc gtaacagtcg gtactaggtg gtctggctac
     3061 gcgtcctgga ccgtccacgt gaaccgcgtg tggctcgagg cgggagcgag cccgccgggc
     3121 cgaaggccct tgcggacgag cagcgtcggc acggacgtgc cctgcgccac ggcaaggcga
     3181 acctcgccga caccacggcg gcgccagtcc gggggcagcc caaagacgac gagtcccatc
     3241 ccctcggccg ccgctgtgag cccctcagcg ttcggctcga cgaggagcgg tgaggtgttg
     3301 atgccgacgg cccgctggac gatcagtgac gcgctcgcga gcaggcggct cgcgtcttcg
     3361 ccctcgctcg cgcggccggc gagcacgagc ggggcgtcgc tggcccgcgc cagccatgcg
     3421 gccagctcca cgccggccca gtcgtgatcc gctccggtaa aggggacaag taccggtcga
     3481 tcggggccga gcggcccgcc ggcacgcacc tccggtagca gcgcgacgtc ggaggacgcc
     3541 gcctcgagca gtgcgaccac gtccggggtc agctctccct cgtggaggat gagatccgcg
     3601 tcctgctccg acgcgagacg tgcgaggtcg cggcccggcg cctgagaggc aaatgcggcg
     3661 gttcgcgcgg ccgcgcccga cgcgatcagg cgctcgcgtc tgctcgccag acgctcgctc
     3721 tcgtcaccca gctccgcggc ggcgactgtc cgcacgagca tcagctcgcg gccgtcaccg
     3781 cttgcgagcg gtgtcgagag cgcgagaagg gtgtcgaggc cacgctcggt cgacgacacg
     3841 atcaggaccg tccgcgcggg cccggccgtc ggcgtctcgg ccgccagcgc cgggtcctgc
     3901 cgcaggattg cccgctcgag ctcctgcagg gccggccccg ggtcgatgcc caactcctcc
     3961 acgagcatct gtcgcgtgtc ctgaaacgca gcgagcgcct cagcctggcg gccggccttg
     4021 tagagggctc gcatcaggtc ggctcgcaac tgctcccggt aggggtgctc ggcgaccagc
     4081 gcccgtagct cctcgacggc ctcctcgggt cgaccgacgt caacgagtgc agccgcgcgc
     4141 cgctcgcgtg cgacgagccg aagctcttcc agcctggcca ctgaggcttc cgcgaagctc
     4201 tcgtgctcga cgttcgccag cgcgggtccg cgccagaggg cgagcgcgtc gtcgagcagg
     4261 gcaagtgcct ctgccggctc cgcctcggct gcagcgtcga cgaggcgctc gaacctccag
     4321 aggtcgagct cgtcggacgc cacgcggagc gcgtagcccg gcgagcgagt ctcgagccgg
     4381 tcggcgccga gcttcttgcg cagttcggcg acacgcatcc gcagcgcgat cgcgccgctc
     4441 cgcggcggct cttcgcccca cacctcgtcg agcaaccggt cgctggacag aaattggttc
     4501 gcgtgcagga gcagcactgc gaggagagcg cgcaatcgcg ctcccctgat gcccacgggg
     4561 ccatcgtcgt cgagcacctc gaggggcccg aggatcctga attccacggc gatttcgacg
     4621 ttagcacggt ccgttacgag tgcgttatcc gcgcgttatt ggcccggcgg atcctgtgct
     4681 cgacgagcca tggagaacgc ctcacgcaag gagcacacaa ccggcgcagc gacgcgcttt
     4741 gcccgccgct cggtcggccc aggagggcag gccggccggc tcgtccgagc gagcattgca
     4801 gcggccgcgg tggtgctcct tgcgctcggg gcgggctgtg ggggcgatcg tgaccacggg
     4861 gccgagcgtt tgatcgagga gagcaccgtg cgcacagcgg gc
//
LOCUS       DBNGNK010000326         3841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1920633,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000326 DBNGNK010000000
VERSION     DBNGNK010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1920633"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="AC150V_09620"
     CDS             <1..836
                     /locus_tag="AC150V_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR3763215.1"
                     /translation="PGDSALVPSPSYPIHLEAPILAGASVTQVPMGYDQDLLENLADA
                     FERASPPPRVLVLSFPHNPTTATIELDEMERVVAFARERNVVVVHDFAYADIAFDGHV
                     PPSILQAAGAEQTAVELYSLTKGFSMAGWRVGFLVGNAEVVQALTRLKSWLDYGTFQP
                     IQIAAITAMNEAPDYPREVCETYRSRRDTLCDGLDRIGWSFPKPRGTMFVWAPIPERY
                     AEMGSLAFAEMLAREAKVAVSPGVGFGPGGEGHVRFALVENDHRIRQAVRGIRQTLTL
                     R"
     gene            complement(841..1311)
                     /locus_tag="AC150V_09625"
     CDS             complement(841..1311)
                     /locus_tag="AC150V_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3763216.1"
                     /translation="MSIPATDFDASLEFYVDVLGLERIPSPNFGFRVEWLRAGDLQVH
                     LYDRGGGDPPSIAQHFGFTVDDFMAVYRRVRARDVSEPQPYFDGVYVLPDGGVQMYTR
                     DPNGNLVELDHPDASVVDRDEVPELRDLADLNPQDETNLASTLFLAQRERQSRS"
     gene            1371..2639
                     /locus_tag="AC150V_09630"
     CDS             1371..2639
                     /locus_tag="AC150V_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR3763217.1"
                     /translation="MYERQLRLLHDRAAADDPLRIGLVGAGTFGQMLLAQARRLPGLD
                     VVAVADLDPSRARRALADAGWEPEGRVVDAAVGVIERAPEVVVEATGSPLAGIAHADL
                     AIQAGCHVVMVTVEADVLGGPLLAEWARAAGVVYSLAYGDQPALICELVGWARATGLD
                     VVCAGKGTKHLPSYHAITPETVWQSYGLTEEQVEAGGFSARMFTSFTDGTKSAVEMAA
                     VCNATGLVPQEEGLHFAPCAADALARTCVPGEAGGLLSRSGTVDVISCLGPDGSRVPG
                     DLRWGVFVVFSTPDARTADWLRAYGIQTSEDGRYGALFRPYHLVGLETTVSVLSVGLL
                     HEATGSPTGLRADVVAVAKRDLAAGEELDGEGGFTVYGGLVPREAARGLLPVGLAEGV
                     RLAEPVAAGSSVPLGALDHAPEHPVLALRR"
     gene            complement(2664..3410)
                     /gene="heR"
                     /locus_tag="AC150V_09635"
     CDS             complement(2664..3410)
                     /gene="heR"
                     /locus_tag="AC150V_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HIR3763218.1"
                     /translation="MNNSRLRAANLGLAVLHAGQAVVIVVIGASLSIPITAQWAEGPP
                     GSALSTTDTLFDLPVRWAVAAFLLMAALDHLLVASPGIVRWYERNLDRGINYARWIEY
                     SFSASLMVVLIATFAGVTDLRALIAVAGVNAAMILFGLLMERTNIGRERLDWLPFWFG
                     CIAGAVPWIVIGVSLVGSEIEGSVPGFVWGIFISLFLFFNSFAVNMWLQYRQVGPWRR
                     YVFGEWGYLVLSLTAKSLLAWQIYSGALAN"
     gene            complement(3440..>3841)
                     /locus_tag="AC150V_09640"
     CDS             complement(3440..>3841)
                     /locus_tag="AC150V_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009747177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR3763219.1"
                     /translation="GTYNGNPLVMAAARANLLEVMTPAAYEHLDALNERIVAGCQAVV
                     DEYRLPGYAIGVASKGCVTFSPVKITDYETFKEHQDEELCDLAWVWNMNRGIFMTPGR
                     EEEWTLSVTHTEADVDHFASVFEEMAQALTS"
BASE COUNT          605 a         1296 c         1353 g          587 t
ORIGIN      
        1 ctcccggcga ctcggcgctc gtcccgtctc cgagctatcc gattcacctg gaggctccga
       61 tcctcgccgg ggccagcgtg acccaggtgc cgatggggta cgaccaggat ctgctcgaga
      121 atctcgccga cgccttcgag cgggccagcc ccccgcctcg cgtgctcgtg ctctcgtttc
      181 cccacaaccc gacgaccgcg acgatcgagc tggacgagat ggagcgcgtc gtggcgttcg
      241 cgcgggaacg gaacgtcgtc gtcgtgcacg acttcgccta tgcggacatc gccttcgacg
      301 ggcacgtccc gccgtcgatc ctccaggccg ccggcgccga gcagacggcc gtcgagctct
      361 actcgctgac gaagggcttc tcgatggccg gctggcgggt cggcttcctc gtcggcaacg
      421 ccgaggtcgt ccaggccctg acccgcctga agagctggct cgactacggt accttccagc
      481 cgatccagat cgccgcgatc acggcgatga acgaggcacc cgactacccg cgggaggtct
      541 gcgagacgta ccggtcgcgg cgcgacacgc tctgcgacgg cctcgaccgg atcggctggt
      601 cgttcccgaa gccgcggggg acgatgttcg tgtgggcgcc gattcccgag cggtacgcag
      661 agatgggctc gctggcgttc gccgagatgc tcgcccgcga ggcaaaggtc gccgtctcgc
      721 cgggcgtcgg cttcggcccc ggcggcgaag ggcacgtccg cttcgcgctc gtcgagaacg
      781 accaccgcat ccggcaggcg gtgcgcggga tccggcaaac gctgacgttg cgctgagcgg
      841 ctacgagcgg ctctgccgct cgcgctgcgc gagaaacagc gtcgaggcca gattcgtctc
      901 gtcctgcggg ttgaggtcgg cgaggtcccg gagctccggg acctcgtccc ggtcgacgac
      961 gctcgcgtcg gggtggtcga gctcgacgag gttcccgttc gggtcgcgtg tgtacatctg
     1021 gacgccgccg tccggcagca cgtacacgcc gtcgaagtac ggctgcggtt cggagacgtc
     1081 gcgggcacga acgcgccggt agacggccat gaagtcgtcg accgtgaaac cgaagtgctg
     1141 ggcgatcgac ggcggatcgc cgccgccgcg gtcgtagagg tggacctgca ggtcgccggc
     1201 gcggagccat tcgacgcgga agccgaagtt gggtgaggga atgcgctcga ggccgagcac
     1261 atcgacatag aactcgagtg acgcatcgaa gtccgtcgcg ggaatgctca cgtggttcaa
     1321 gcctgtgacc cgcccagcca ttgtgagttc aatcctgaca ggatcgcctc gtgtacgagc
     1381 gccaactgcg actcctccat gatcgcgcgg ctgccgacga tccgctccgg atcggactcg
     1441 tgggggcggg taccttcggg cagatgctgc tcgcccaggc ccgcaggctg cccgggctcg
     1501 acgtcgtggc ggtcgccgat ctcgacccgt cgcgggcacg ccgcgctctc gccgacgcag
     1561 gctgggagcc ggagggacgc gtcgtcgacg ccgcggtggg cgtcatcgag cgcgcgcccg
     1621 aagtcgtcgt cgaggccacc ggctcgccgc tcgcgggaat cgcccacgcc gatctggcga
     1681 tccaggccgg ctgccacgtc gtgatggtca ccgtcgaggc ggacgtgctc ggggggccgc
     1741 tgctcgcgga gtgggcgaga gctgccggcg tcgtctacag cctcgcctac ggagaccagc
     1801 cggcgctgat ctgcgagctc gtcggctggg ctcgggcgac ggggctcgac gtcgtctgtg
     1861 ccgggaaggg gacgaagcac cttccctcct accatgcgat cacgcccgag acggtctggc
     1921 agagctacgg cctcaccgag gagcaggtcg aggcgggtgg attcagcgca cggatgttca
     1981 cctcgttcac agacgggacg aagtcggccg tcgagatggc cgcggtgtgc aacgcgaccg
     2041 ggctcgtgcc gcaggaggag gggctccatt tcgcgccgtg cgcggccgac gcgctcgccc
     2101 gcacttgcgt gcccggcgag gcaggcggcc tgctgtcgcg aagcggcacg gtcgatgtga
     2161 tctcgtgtct ggggcccgac ggcagccgcg tcccaggcga ccttcgctgg ggtgtcttcg
     2221 tcgtcttctc gacgcccgac gcacggacgg cggactggct gcgggcgtac ggcatccaga
     2281 cgagcgagga cggtcgctac ggagcgctct tccgccccta ccacctggtc ggcctcgaga
     2341 cgacggtcag cgtcttgtcg gtcggactcc tgcacgaggc gaccggctcc cccaccggcc
     2401 tccgtgccga cgtcgttgcg gtcgcgaagc gcgatctcgc agcgggcgag gaactcgacg
     2461 gcgaaggggg gttcaccgtc tacggcggac tggtcccgcg ggaggcggcg cgcgggctgc
     2521 tgcctgtcgg tctcgccgag ggggtcaggc tcgcagagcc tgtcgcagcc ggctcttccg
     2581 ttccactcgg cgcgctcgac cacgcacccg agcatccggt gctcgccctt cgacggtgac
     2641 ggccgcggca gcggcgtgac agctcagttg gcgagagcgc cggaatagat ctgccaggcg
     2701 agcaggctct tcgcggtcag gctgagaacg aggtaccccc actcgccgaa cacgtagcgg
     2761 cgccacgggc cgacctggcg gtactgcagc cacatgttga ccgcgaagct gttgaagaag
     2821 aggaagagcg agatgaagat cccccagacg aagcccggga cgctgccttc gatctcggag
     2881 ccgacgagcg agacgccgat gacgatccaa gggacggcgc ctgcgatgca gccgaaccag
     2941 aacgggagcc agtcgagccg ctcgcggccg atgttcgtcc gctccatgag caagccgaag
     3001 aggatcatcg ctgcgttcac tccggcgacc gcgatcagcg cccgcagatc cgtcacgccg
     3061 gcgaacgtcg cgatcaggac gaccatcaga gacgcgctga acgagtactc gatccagcgc
     3121 gcgtagttga tcccgcggtc gagattccgc tcgtaccagc ggacgatccc gggtgacgcg
     3181 acgagcaggt ggtcgagcgc ggccatcaga aggaacgccg cgacggccca gcgcacgggc
     3241 aggtcgaaga gcgtgtcggt tgtgctgagt gccgagccgg ggggcccctc ggcccactga
     3301 gccgtgatcg ggatcgagag cgacgcgccg atgacgacga tgacgacggc ctggccggcg
     3361 tgaagcaccg ccaggcccag gttcgcggca cggagacggg aattgttcac ggcccgatcc
     3421 tatacaaaat ctctacgaac tacgaggtga gcgcctgtgc catctcctcg aagaccgagg
     3481 caaagtggtc gacgtcggcc tcggtgtgcg tcaccgacag cgtccactcc tcctccctgc
     3541 ccggcgtcat gaagatgccg cggttcatgt tccagaccca cgccaggtcg cacaactcct
     3601 cgtcctggtg ctccttgaac gtctcgtagt cggtgatctt gaccggcgag aacgtgacgc
     3661 agcccttgga ggcgacgccg atcgcgtagc cgggaagccg gtactcgtcg acgacagcct
     3721 gacagccggc gacgatgcgc tcgttgagcg catcgagatg ctcgtacgcg gcgggggtca
     3781 tgacctccag caggtttgcc cgcgcggccg ccatcacgag cgggttgccg ttgtacgtgc
     3841 c
//
LOCUS       DBNGNK010000327         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1702789,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000327 DBNGNK010000000
VERSION     DBNGNK010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1702789"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..967)
                     /locus_tag="AC150V_09645"
     CDS             complement(104..967)
                     /locus_tag="AC150V_09645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR3763220.1"
                     /translation="MTSTAGSTTPSARAIGRIAVLTHGNPAQIGSALDRVHAVAQSAG
                     VDVLVSRDEAHKHGVDASKNEADGAELAIVLGGDGTMLRALTRYLGTGVPVIGVNFGR
                     VGFLSSISQEGLESGLARVFAGEYEVVELPTIELEVAGDRHVAVNDAVVSSGVLGRMV
                     ELDWSVGGEELGLVPCDGLICATPSGSTAYNLSNGGPLLMWGIDAMAITFVAPHSLHA
                     RPFVVPRGRDVVVWNRTADVPVSVLVDGHAVAEAAPDERVVVRLGAERSLLATLPEAT
                     FVRRYRTSFAP"
     gene            complement(930..1691)
                     /locus_tag="AC150V_09650"
     CDS             complement(930..1691)
                     /locus_tag="AC150V_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR3763221.1"
                     /translation="MKKRVDVLLHERGLAESRSQAQALVLAGLVEGIEKPGTQVDEQA
                     ELTVLSPPRFVSRGGEKLATGLDAFGIDVAGLDCLDVGASTGGFTDCLLQRGAARVIA
                     LDVGYGQLHPKLRDDPRVIVLERTNARDVRELPFPPELVTCDVSFISACVALPPLLAL
                     ARPGWQAVILVKPQFEAGRADAPKGVVRDPEVQRKVLESVAKAAIAWDGQVVGVVDSG
                     LPGPKGNREFFLHIVNQFEPTVLDDIDRWIDDAVG"
BASE COUNT          318 a          645 c          615 g          279 t
ORIGIN      
        1 ccgtgaaacg ggactaaaac gggactatcg cgggtttccg gccctctccc gacatgagaa
       61 ccccccggta atcgggggtt tctcatcggg cgtgattgct tgcctacgga gcaaagctcg
      121 tgcgatacct gcggacgaag gtcgcctccg gcagcgtcgc cagcagcgac cgttccgcgc
      181 cgagacggac gactacgcgc tcgtcgggcg ccgcttcggc gacggcgtgg ccgtcgacca
      241 gaacgctgac cggtacgtcg gccgtgcggt tccagacgac gacgtctcgc ccacgcggca
      301 cgacgaaggg ccgcgcgtgg agagagtgag gcgcaacgaa ggtgatcgcc atggcatcga
      361 tgccccacat gagcagcggc ccgccgttcg agaggttgta ggccgtcgag ccggacgggg
      421 tcgcacagat caagccgtcg cacgggacga gcccgagctc ttcgccccct accgaccagt
      481 cgagctcgac catgcgcccg agcacgccgc tcgagacgac cgcgtcgttc acggcgacgt
      541 gccggtcgcc cgcgacttcg agctcgatcg tcggcaactc gacgacctcg tactcgcctg
      601 cgaacacacg agcgagccca ctctccagtc cctcctgcga gatcgagctc aggaagccga
      661 cacgaccgaa gttcacgccg atgacgggaa cgccggtgcc gaggtagcgc gtcagggcgc
      721 gcagcatcgt cccgtcacct ccgagcacga ttgccagttc ggccccgtcc gcctcgttct
      781 tcgacgcatc gacgccgtgc ttatgcgcct cgtcccggga gacgaggacg tcgacgccgg
      841 ccgactgcgc cactgcatgc acgcggtcga gtgcagagcc gatctgcgcc gggttgccgt
      901 gcgtgaggac ggcgatgcgt ccgatcgccc tagccgacgg cgtcgtcgat ccagcggtcg
      961 atgtcatcga gaactgtcgg ttcgaactga ttgacgatgt ggaggaagaa ctcccggttt
     1021 cccttggggc cgggcaagcc agagtctacg acgccaacaa cctgcccatc ccacgcgatc
     1081 gcggctttcg caacgctctc gagtactttt cgttgcacct ctgggtctct cacgacgccc
     1141 ttcggcgcgt ccgcgcggcc tgcctcgaac tgcggcttga cgagaatcac ggcctgccag
     1201 cccggccgtg ccagcgcgag cagcggcggc agggcgacgc aggccgagat gaaggacacg
     1261 tcgcacgtga cgagctcggg aggaaacggg agctcgcgca cgtcacgcgc gttcgtccgc
     1321 tcgagcacga tcacccgcgg gtcgtcgcgc agcttcgggt gcagctggcc gtagccgacg
     1381 tcgagggcga tcacgcgcgc ggcgccgcgc tgcaacaggc agtcggtgaa cccgccggtg
     1441 gatgctccca catcgagaca atccagaccg gcgacgtcga tgccgaaggc gtcgagaccc
     1501 gtcgccagct tctcgccgcc acgggacacg aaccgcggtg gcgagagcac cgtgagctcc
     1561 gcttgctcgt ccacctgggt tcccggtttc tcgatgccct cgacgagacc ggcgagtacg
     1621 agcgcctgag cttgggaacg actctccgcc aggccgcgct cgtgtaagag aacatccacg
     1681 cgcttcttca cgagtcaagg ctagttgacc ggccggcgtc ctccggggcg gagaccggtc
     1741 ggcgatgaga cgcgagccgg tgtcggccgt cgcgcgggcg gtcgagagga atgcccagcc
     1801 cctgctcccg aacggcggcc tctaccgccg atggagtctc tgacacagac agggtgc
//
LOCUS       DBNGNK010000328         3145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_846782,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000328 DBNGNK010000000
VERSION     DBNGNK010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_846782"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="AC150V_09655"
     CDS             complement(<1..618)
                     /locus_tag="AC150V_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3763222.1"
                     /translation="MGRLLYRVGSLAARHHWFFLIGWLAVAAAVVVLILTYGSNTSDN
                     LSLPGTDSQAATDLLAARFPPQQNGASPVMFAVESGKVTDAANKQAIESSRAAIVKLP
                     NVFSATDPFSQQGQAQISKDGTIAFIPVLLGVSGDALTEEVAESVLDAAEPARKAGME
                     VAVGGPIGSELSEPTTEGSDLIGLIAAMLILTFAFGSIVAMGMPIL"
     gene            complement(767..1156)
                     /locus_tag="AC150V_09660"
     CDS             complement(767..1156)
                     /locus_tag="AC150V_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763223.1"
                     /translation="METSSGAPSSDPPALVNARLVQHPDGGSEFLLATTEAGDHVAFG
                     FEVESDAPGLVLVDPRLWLEAGEEGTPELSELRFEATEEAWADDWLPALLAHPVAVEW
                     LTRIKQQHPDAYESWFAQEDYEEGGAG"
     gene            complement(1211..2005)
                     /locus_tag="AC150V_09665"
     CDS             complement(1211..2005)
                     /locus_tag="AC150V_09665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR3763224.1"
                     /translation="MAERPTTIVQISDLHCGSPFFRPELLERAIVEINGLGPDIVVCT
                     GDLTTDGFRGEFREAKEYLDRIACETLVVVPGNHDSRNVGYVHFDDFFGHRGSVFHDE
                     RVSLVAVDSSEPDIDGGHIGRTRYGWIMEQFERPAQLRVFVLHHHLVAVPGTGRERNV
                     VSDAGDLLETLLRAKVDLVLCGHKHVPYAWRVEELFVVNAGTVSSLRLRGKTQPCYNI
                     VELAGGWVTVWLKYPFLGRERILRFSLETLEYEKTTERIDHEVTMH"
     gene            complement(2117..>3145)
                     /gene="fahA"
                     /locus_tag="AC150V_09670"
     CDS             complement(2117..>3145)
                     /gene="fahA"
                     /locus_tag="AC150V_09670"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - L-tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HIR3763225.1"
                     /translation="AGLGPAVEGTTLDRLLAAGRGAWEEMTGLALDALSGGAAPTHPL
                     AQVSSHLPFTVSDYVDFYSSLEHAANLGRMFRSDSDALLPNWLWLPVGYHGRSGTVVV
                     SGSDVVRPWGQSRADGESSPSYGASARLDFELELGFVVGTPSPLGVPVALEEFEEHVF
                     GVALLNDWSARGIQAWEYEPLGPFLGKSFQTSIAAWVTPLTLLADARVEGPVQEPEPL
                     AHLSGGRDWALDLVLQIELNGTVISRGNARGLYWTMPQQLAHVTSNGASLRTGDLLAS
                     GTISGAERGSEGSLIELTANGGEPLLLADGTTRSYLEDGDEVVLRGHGPGVVLGETRG
                     RIVPAVGD"
BASE COUNT          528 a         1087 c         1054 g          476 t
ORIGIN      
        1 cagaatcggc attcccatcg cgacgatcga tccgaacgcg aaggtgagga tgagcatcgc
       61 cgcaatcagc ccgatcagat cgctgccctc ggtcgtcggc tccgacagct cgctgccgat
      121 cgggccaccg accgcgacct ccattccggc cttgcgcgct ggctccgcgg cgtccaggac
      181 gctttcggcg acctcctccg tcagggcatc gccgctgacg ccgaggagca cgggaatgaa
      241 ggcgatagtg ccgtccttcg agatctgagc ctgaccctgc tggctgaagg ggtcggtcgc
      301 gctgaagacg ttcggcagct tcacgatcgc cgcccgcgac gactcgatcg cctgtttgtt
      361 cgcagcgtcc gtcaccttgc cgctctcgac ggcgaacatg accgggctgg cgccgttctg
      421 ctggggcggg aaccgtgcgg ccagcaggtc cgtcgcggct tggctgtcag ttccggggag
      481 gctgaggttg tcgctcgtgt tggacccgta ggtgaggatc aacaccacca ccgccgccgc
      541 cacggcaagc cacccgatca gaaagaacca gtggtgccgc gctgcaagcg accccacgcg
      601 atacagcagc cgccccactc gacctcccga ggttgtcgcc cacgatcacg cccggattct
      661 cgaatccaac gaaacactac aaagccgccg ccggacgagg caatcgggcg gctcccgagg
      721 gttcgcgatc agcgtcgctc gtcgcgaggt cccggccgtg gaaccctcaa ccggcgccac
      781 cctcctcgta gtcctcctgc gcgaaccagg actcgtaggc gtctgggtgc tgctgcttga
      841 tgcgcgtgag ccactcgacc gcgacggggt gggcgaggag cgcggggagc cagtcgtccg
      901 cccaggcctc ctcggtggct tcgaaccgca actcgctcag ctccggcgtc ccctcctccc
      961 ccgcctccag ccacagccgc ggatcgacga ggacgaggcc gggcgcgtcg ctctcgactt
     1021 cgaagccgaa cgcgacgtga tcgccggcct cggtcgtcgc gagcaggaac tcgctgccgc
     1081 cgtcggggtg ctgcacgaga cgcgcgttga cgagcgccgg ggggtcggaa ctcggagcgc
     1141 ccgaactcgt ttccacggcc ggacgataac gcggcctcgg tcgggctgcg tgaagccttc
     1201 gccacggcgc ctagtgcatt gtcacctcgt gatcgatccg ctcggttgtc ttctcgtact
     1261 cgagcgtctc gagcgagaag cggaggatgc gctcgcgtcc gagaaacggg tacttgagcc
     1321 aaaccgtcac ccagccgccg gcgagctcca cgatgttgta gcagggctgt gtctttcctc
     1381 tcaggcggag agaggagacc gtacccgcgt tgaccacgaa gagctcctct acgcgccagg
     1441 cgtacggaac gtgcttgtgc ccgcagagca cgagatcgac cttggcgcgc agcagagtct
     1501 cgaggagatc gccggcatcg gagacgacgt tgcgctctcg ccccgtcccc ggcactgcga
     1561 cgaggtggtg gtgcagcacg aacacgcgta actgggcagg acgctcgaac tgctccatga
     1621 tccagccgta gcgcgtgcgt ccgatgtgac ctccgtcgat gtccggctcg gaggagtcga
     1681 ccgcgacgag cgaaacgcgc tcgtcgtgga acacggagcc gcggtgtccg aagaagtcgt
     1741 cgaaatgcac gtagccgacg tttcgggagt cgtggttgcc cggcaccacg accagcgttt
     1801 cgcatgcgat ccggtcgagg tactccttcg cctcgcgaaa ctctccgcga aacccgtccg
     1861 tggtcagatc gccggtgcac acgacgatgt ccggcccgag accgttgatc tcgacgatcg
     1921 cccgctccag gagctcgggg cgaaaaaacg gcgagccgca atgaaggtct gagatctgga
     1981 cgatcgtcgt gggtcgctca gccatgtcta ggcagggatt cgaaccggca ctggatcccc
     2041 acttccgcag cgggcacgcg gacggtcgct ccggtcgggc tggtctgctc cattgcgaag
     2101 ggcaacaagc gtcaccttag tcgccgacgg ccgggacgat gcgcccacga gtctcgccga
     2161 gcacgacccc cgggccgtgt ccgcgcaaga cgacctcgtc tccgtcctcc aggtacgagc
     2221 gggtcgtgcc gtcggcgagg agcagcggct ccccgccgtt cgcggtcagc tcgatcagcg
     2281 agccctcgct gccgcgctcg gcgcccgaga tcgtgcccga ggccagcaga tcgccggtgc
     2341 gaagcgacgc tccgttggac gtgacgtgag cgagctgctg cggcatcgtc caatagaggc
     2401 cgcgggcgtt gccgcgcgag atcaccgtgc cgttgagctc gatctggagc actaggtcga
     2461 gggcccagtc gcgccctccc gacaggtgtg cgagcggctc gggctcctgc acagggccct
     2521 cgacacgagc gtccgcgagg agcgtgagcg gcgtcaccca ggctgcgatc gacgtctgga
     2581 aggacttgcc gaggaacggc ccgagcggct cgtactccca cgcctggatg ccgcgcgcgg
     2641 accagtcgtt gaggagcgcg acgccgaaga cgtgctcttc gaactcctcc agcgcgaccg
     2701 ggactccgag agggctcggc gtcccgacga cgaagccgag ctcgagctcg aagtcgagcc
     2761 gcgcactggc cccgtaggac ggcgacgact caccgtcggc gcgcgactgc ccccatggac
     2821 gcacgacgtc cgagcccgag acgacgacgg ttcccgagcg accgtgatag ccgaccggga
     2881 gccagagcca gttcggcagc agagcgtccg agtccgatcg gaacatgcgg ccgaggttgg
     2941 cggcatgctc gagcgacgag tagaagtcga cgtagtcgga gacggtgaac ggcagatgag
     3001 agctgacctg ggcgagcggg tgggtcggcg ccgccccacc ggatagcgcg tccagggcca
     3061 gccctgtcat ctcctcccag gcgccgcgac cggcggcgag caaccggtcg agtgtcgtcc
     3121 cctctacggc cggccccagc cccgc
//
LOCUS       DBNGNK010000329        10061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1992697,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000329 DBNGNK010000000
VERSION     DBNGNK010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1992697"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="AC150V_09675"
     CDS             complement(<1..582)
                     /locus_tag="AC150V_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HIR3763226.1"
                     /translation="MPPAGFWTDMLDPSWPELESAAPAELLRPARVELEAPARPETEP
                     RPTLESHGGYLVGVLLVPVALPEEDRLVFQEVDLVVTGEAVLTVRKSSGDDPPLDADV
                     IAEIVGASRASPGRFVRLLVDEVAERYLQAIDALEDEIDELEGGIGAWPSDRIQRRAV
                     GVRRAIIRLRRVVAPTRDAVRRVVDGRIEVAGAP"
     gene            829..3429
                     /locus_tag="AC150V_09680"
     CDS             829..3429
                     /locus_tag="AC150V_09680"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR3763227.1"
                     /translation="MRRRHAQGLVSSLVLSGSLLLVAACGLVLIGHERASILPAPAIP
                     WPLLGVGFVLAQLAVVRFSYRGLEGSASMGAFPLVLGLLFAPVWAVLAAGLVGTGIAF
                     VLRRESASEVVVRLAAFAAGAVTAVLVYDAVLGNAGTFDPRGLAAMCAAAASAGALGG
                     IASYLAVRLAEEDEGIAELALYVATGLAVAAAAASLALAAGALLVVDRYLALLLVPPV
                     ALVALAYRVFAASQGQRDRLEFLMSLTRAVNEAPRVEDAVLALLEHLRRRFDVSVGAM
                     TLLPAGDNESALLAQLGPSRSMRDVRRLDAVRSARVAAVVSSLEGAVRGKPSASPGAE
                     LQTLVDADLAEMAVVPVAGRTRTVGALVLANTAGEGGSFAKKDLAFLAEIGAHAGVAL
                     EAGRLEKSLEQLVHLKEQLHHTAYHDALTGLPNRALFNERLSHAVTRQARAPRPLAVL
                     FIDLDDFKAANDTYGHETGDWLLTSAAERLESSLRDEDSIARLGGDEFAILLEETPAD
                     EVPRIADRLVAELNRPFEIGGGEEINLSASIGVALSPGGRSADELLRNADVAMYRAKA
                     QGKARHNLYDEDMREKIFRPLQLKRRLEGAIARGELDLHFQPIYDLATRHMTGAEALL
                     RWNDEELGAVSPGEFISLAEETGLIVPMGRWALARACETARIWQRQAARAPWVSVNVS
                     ANQLEDRKFPDLVARTLADNGLDPSALVLEVTESVCLTDDGSAARAFAILQKLGVRIA
                     LDDFGSGYSSLGVLQGYTVSILKLDRTFIAPLHGERDTPLLREMLRMANSLNLLTVGE
                     GIERVEQLDRLKELGCRLGQGYLLSKPMEGVAVQALLGKQVVVRDSETPSGGRRPVAS
                     PSTQAQPAVS"
     gene            complement(3507..4841)
                     /locus_tag="AC150V_09685"
     CDS             complement(3507..4841)
                     /locus_tag="AC150V_09685"
                     /inference="COORDINATES: protein motif:HMM:NF016212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3763228.1"
                     /translation="MSQAVKNYRRVTPERMHDVRANARRLGVLYSVEVELSRLIGGWI
                     SRVPELPEKLALGLMLYEDADHAHELEARLVELMIRPADFEHLRRRSAPGLVALERAE
                     GPESFLASLFGVVKPALLADLRRHLEGCPPYVDEPTVRMLKRIIADQEEHIATGLALL
                     ADRGVAPAGPGADPLWDLFADDGGIVAGSFVGADPVPLTSPVWPAAVEKLSSGDPTPP
                     YPGDYDADMRRCIHDLVFSETEALDIFGRYVYEFHRLPWEFHRESARIAWDEARHVEL
                     LLNVLERYGGEIGEFPAKAPGYEEFVREPTTLRKILMVNVIAEGEVSTDTQTQHREAF
                     RQLGDELSAILKDYEMADEVVHGQFGVKWSRWLARESGEDYDEQLAAARRSLEEFKSS
                     HDDEGESEIPLVRLGADETGSRRVVNVAAKRLLGYSDEEIERMASSATTIDV"
     gene            complement(4864..5328)
                     /locus_tag="AC150V_09690"
     CDS             complement(4864..5328)
                     /locus_tag="AC150V_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3763229.1"
                     /translation="MGSETIVVGFDGSPGSEAALQWAVEEAEHRGAALKVVYVWHVPL
                     VVGLPEPGVLGRPVLSQPPLEGVIDDYQRRAEHVLDDALAELEGVEAERVLKEGNAAR
                     ELIEASADADLLVVGSRGHGGFTGLLLGSVSQQCAHHAQCPVVIVPPPEEAR"
     gene            5475..5681
                     /locus_tag="AC150V_09695"
     CDS             5475..5681
                     /locus_tag="AC150V_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018174172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR3763230.1"
                     /translation="MASGTVKWFNDAKGYGFITPDEGSKDLFVHHSNIAGEGFKSLTE
                     GARVEFEQREGEKGPEAINVATAA"
     gene            5760..5981
                     /gene="infA"
                     /locus_tag="AC150V_09700"
     CDS             5760..5981
                     /gene="infA"
                     /locus_tag="AC150V_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR3763231.1"
                     /translation="MAEKEEKLEMEGEIIESLRNRMFRIRLDNGHEIIGYTSGRMKRF
                     RIRMMLGDRVRVEVSPYDLNRGRIVYRMG"
     gene            6047..6256
                     /locus_tag="AC150V_09705"
     CDS             6047..6256
                     /locus_tag="AC150V_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763232.1"
                     /translation="MDIKVTLRTGNEYVLVPGNSERDVEDLFTELAAGRSQALRGWVP
                     VASEKGDRIVVRGDEIVELHLVGGD"
     gene            6266..6562
                     /locus_tag="AC150V_09710"
     CDS             6266..6562
                     /locus_tag="AC150V_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763233.1"
                     /translation="MATDAVGEEAGIAHRVASRRANERRAAAKNPPTDGQGLVEFACE
                     CITTDCERSVKVPLYVYERLVESGDQYLVQAAHHAFARHRTIVTLGLMRIEERA"
     gene            complement(6611..7021)
                     /locus_tag="AC150V_09715"
     CDS             complement(6611..7021)
                     /locus_tag="AC150V_09715"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR3763234.1"
                     /translation="MATEHEALVRRFYDEIVNERKLELIDELVSPDFVEHEEFPGLAA
                     GRDGIKEFFGIMLDAFPDMRFEVEDIITTEDTAVVRLRMRGTHQGTFMDIPATGKKID
                     VATIDWVRIRDGVVVEHWGVTDTGAMMQQLGVAG"
     gene            7184..7606
                     /locus_tag="AC150V_09720"
     CDS             7184..7606
                     /locus_tag="AC150V_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIR3763235.1"
                     /translation="MPHFENLPAAEFGFPGPLRDRLVAAILSGDKTSTTGLLEEYRRE
                     ATPVEEVGLRERVVDSEGRSVAVIETTEVEVKRLGDVDLTFAIDEGEGFATLDEWRAA
                     HVRFFTSAEMLAALGEPPVPIDDDTLVVCTRFRVVERL"
     gene            complement(7616..8524)
                     /locus_tag="AC150V_09725"
     CDS             complement(7616..8524)
                     /locus_tag="AC150V_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR3763236.1"
                     /translation="MAHEQVQPVPGDGPPGSLRASVGLVRRAVTRAFADEVTDSAAAV
                     AYYTFLAIPAVLLVAVGIFSLAAGPSAIERILERLEGVMPAEAIALIDDSLTRATESQ
                     TGPLLMIIVGLLLALWTSIGAMNALMRRLNHIYAREETRGFVKQRAVGLAMLAWAFLA
                     FALAFGFLVLGPHLSGWIGSALGIESAVTWIWWSAQWPILIAGLLAAVAGILALGPNT
                     DGRSGWRFLSLGSVITVIVFLLASGLFSLYVSRFGSYNKAWGSLAAVVILLTWLWLSA
                     LALLLGGAVNAESDRRAEPARTPTSR"
     gene            complement(8591..8821)
                     /locus_tag="AC150V_09730"
     CDS             complement(8591..8821)
                     /locus_tag="AC150V_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763237.1"
                     /translation="MSATPAKGGGEQIKMGLTDTIKGWFSKGAEAAGDAAGKATDVAG
                     DVAESAKDVGEQAVDKAKDVAGQGDDQADETP"
     gene            complement(8960..9157)
                     /locus_tag="AC150V_09735"
     CDS             complement(8960..9157)
                     /locus_tag="AC150V_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763238.1"
                     /translation="MPDEGRTTEEIRRDITAEREQLTQALADLREGIDAKRRPAAAVV
                     GAVATGLVVVAALNVARRLVG"
     gene            complement(9147..9593)
                     /locus_tag="AC150V_09740"
     CDS             complement(9147..9593)
                     /locus_tag="AC150V_09740"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR3763239.1"
                     /translation="MQDENASDRATTAAGESGSTARPRGIRASAKRAVEHARSLATLQ
                     RELARQELKHKGATVGAGAALAVAAGIFMLFAVGFGLAAVAAALALVVDWWLALLIVF
                     VLLLLLVLGLALASRALFREGTPLKPEQAIEEARLTKQALRGNRAG"
     gene            complement(9655..9936)
                     /locus_tag="AC150V_09745"
     CDS             complement(9655..9936)
                     /locus_tag="AC150V_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763240.1"
                     /translation="MAVDETEFAIALSRPAVGAGEVTFNVYNRGMDDHDLAVVDAGGN
                     LKVVAVPPGETRTLSVQLSAGTVKLYCSLFGGTPASHEDLGMVTYLDVR"
BASE COUNT         1587 a         3466 c         3438 g         1570 t
ORIGIN      
        1 cggcgcgccg gccacctcga tccgcccgtc caccacgcga cggactgcat cgcgggtcgg
       61 cgcgacgacg cggcgcagcc ggatgattgc gcgccgaact ccgaccgccc gtcgctgaat
      121 tcgatccgac ggccaggcgc cgatcccgcc ctcgagctcg tcgatctcgt cctcgagcgc
      181 atcgatcgcc tgaaggtagc gctcggcgac ctcgtcgacg aggagccgga cgaaccgtcc
      241 cgggctcgct ctcgaggccc cgacgatctc tgcgatcacg tcggcgtcga gcggcggatc
      301 gtcgcctgac gatttccgga ccgtgagaac cgcctcgccg gtaacgacga ggtcgacttc
      361 ctggaagacc aagcgatcct cctcaggaag tgcgaccggc acgaggagaa cgccgacgag
      421 gtagccgccg tggctttcga gcgtcggtcg cggctccgtc tccggtctcg ccggcgcctc
      481 gagctctacc ctggccggcc gcaggagctc ggctggcgca gccgactcga gctcgggcca
      541 cgacgggtcg agcatgtcag tccagaaccc cgctggaggc acccgccgag cctagcgcgg
      601 cgcccggctg ggccacggcg gcgcagcagg gtaccccgaa cggggcatgc cgcggggtgc
      661 catcgcctct caatgtgggt ggtgcgacaa gcgagtcgcc gacggcactg cggccggacc
      721 tacgcaggtc cggagaaggc gagcagcacg atgcgcggac acaggcgcct ggcaagcaaa
      781 ccgagacacg gagcacgtac gcctccgccg gaaggcgggt agctcgtcat gcgccgccgc
      841 cacgcacaag gtctcgtctc gtcgctggtg ctcagcggga gcctcctgct cgtcgccgcc
      901 tgcggcctcg tcctcatcgg ccacgaacgc gcctcgatcc tgccggcgcc ggcgatcccg
      961 tggccgctgc tcggggtcgg cttcgtcctc gcacagctcg ccgtggtgcg tttctcgtac
     1021 cgcggcctcg agggcagcgc ctcgatgggt gcattcccgc tcgtgctcgg tctgctcttc
     1081 gcgcccgtct gggcggtgct cgcagccggg ctcgtcggca ccggaatcgc gttcgtcctc
     1141 cgccgcgaga gcgcgagcga ggtcgtcgtc cgcctcgccg ccttcgcggc cggagcggtg
     1201 accgccgtcc tcgtctacga cgccgtgctg ggaaatgcgg gaaccttcga tccgcgtggg
     1261 ctcgctgcga tgtgcgctgc ggcggcgtcg gcgggtgctc ttggcggaat cgcgtcatac
     1321 ctcgcagtac gactcgcgga ggaagacgaa ggaatcgccg agctcgcgct gtacgtcgcg
     1381 acaggactcg cagtcgcggc cgcggccgcc tcgctcgctc tcgctgccgg cgctctgctg
     1441 gtcgtcgatc ggtaccttgc gctgctgctc gtccctccgg tggcgctcgt ggcgctcgca
     1501 tatcgcgtct tcgcggccag tcagggccag cgcgaccgac tggagttcct gatgtccctg
     1561 acacgcgcgg tgaacgaggc gcctcgggtc gaggacgcgg tgcttgccct gctcgagcac
     1621 ctgcgccggc gcttcgacgt cagcgtcggc gccatgacgc tgctcccggc aggcgacaac
     1681 gagtccgccc tgcttgctca gctcggccca tcacgctcga tgcgtgacgt gcggcgactg
     1741 gacgcggtgc gcagcgccag agtggcagcg gtcgtcagct cgctcgaagg agcggttcga
     1801 ggcaagccgt cggcgagccc gggagccgag cttcagacgc tcgtcgacgc cgatctggcc
     1861 gagatggctg tcgtgcccgt ggcagggagg actcggacgg tcggcgcgct cgtgctcgcc
     1921 aatacggccg gggaaggcgg gtcgttcgcg aagaaggatc tcgcgttcct ggcggagatc
     1981 ggagcgcatg ccggcgtcgc gctcgaggcg ggccgcctgg agaagtcgct cgagcagctc
     2041 gtccatctga aggagcagct gcaccacacc gcctaccacg acgcactcac cggacttccc
     2101 aatcgggcgc tcttcaacga gcggctctcg cacgccgtga cgcggcaggc tcgagcaccc
     2161 cggccgctcg ccgttctgtt catcgacctg gacgacttca aggccgccaa cgacacgtac
     2221 ggccacgaga cgggcgattg gcttctgacg tcggcggcgg aacgtctgga gtccagtctg
     2281 cgcgacgagg actcgatcgc gcggcttggc ggcgacgagt tcgcgatcct cctcgaggag
     2341 actcccgccg acgaggtacc gagaatcgcc gaccgtctcg tcgcggagct caaccggccc
     2401 ttcgagatcg gcggcggcga agagatcaat ctctcggcga gcatcggtgt ggcgctctcg
     2461 cccggaggcc gttctgccga cgagctgctt cggaacgcgg atgtcgcgat gtatcgggct
     2521 aaggcgcagg ggaaggcccg ccacaacctg tacgacgagg acatgcggga gaagatcttc
     2581 cggccgctcc agctgaagcg acgcctcgaa ggcgcgatcg cccgcggcga gctcgatctc
     2641 cacttccagc cgatctacga tctggcgacg cggcacatga cgggtgcaga ggccttgctg
     2701 cgctggaacg acgaggagct cggcgcggtg tcacccgggg agttcatctc cctggcggag
     2761 gagacaggac tgatcgtccc gatggggcgc tgggcgctcg cccgcgcgtg cgagacggca
     2821 cggatctggc agcggcaggc tgcgcgcgcg ccgtgggtga gcgtcaacgt ctcagccaat
     2881 cagctcgagg atcgcaagtt cccggacctg gtggcgcgaa ccctggcgga caacggcctc
     2941 gaccccagcg cgctcgtcct cgaggtcacg gaaagcgtgt gcctcaccga cgacggcagc
     3001 gcggcccggg cgttcgccat cctgcaaaag ctcggcgtcc ggatcgctct cgacgacttc
     3061 ggcagcggct actcgtcgct cggcgtgctc caggggtaca cggtctcgat cctgaagctc
     3121 gatcgcacct tcatcgcgcc gctgcatgga gagcgcgaca ccccgctgct tcgcgagatg
     3181 ctgcggatgg caaactcgct caatctgttg accgtcggcg aggggatcga gcgggtcgag
     3241 cagctcgacc ggctgaagga gctcggctgc cggctgggac agggctacct gctgagcaag
     3301 ccgatggaag gggtcgccgt gcaagccctg ctcggcaagc aggtcgtcgt ccgcgattcg
     3361 gaaacgccct cgggcggacg tcggccggta gcttcaccct ccacccaggc gcagcccgcg
     3421 gtcagctaga cagtcgcttc gctgcggcgg acgactgcgg ggctcgcgac actcctgcgg
     3481 ctctgatggg cacgggccgg tcgctgctag acgtcgatcg tggtcgccga cgacgccatc
     3541 cgctcgatct cctcgtcgct gtagccgagc aagcgcttgg ccgcgacgtt gacgacgcgg
     3601 cggcttccgg tctcgtcggc gccgaggcga acgagcggga tctccgactc gccctcgtca
     3661 tcgtggctgg acttgaactc ctcgagcgat ctgcgcgccg cggcgagctg ctcgtcgtag
     3721 tcctcgcccg actcccgcgc gagccagcgc gaccacttga cgccgaactg gccgtgcacg
     3781 acctcgtcgg ccatctcgta gtccttcagg atcgccgaga gctcgtcgcc gagctgccgg
     3841 aacgcctcgc ggtgctgtgt ctgggtgtcg gtggagacct cgccctcggc gatgacgttc
     3901 accatcagga tctttcgcag cgtggtcggc tcgcgcacga actcctcgta gccgggcgct
     3961 ttcgccggga actcgccgat ctcgccgccg tagcgctcga ggacgttgag caggagctcg
     4021 acgtgccgcg cctcgtccca cgcgatccgg gccgattcgc gatggaactc ccatggcagc
     4081 cggtggaact cgtagacgta gcgtccgaag atgtccagcg cctccgtctc gctgaaaacg
     4141 aggtcgtgga tgcagcgccg catgtctgcg tcgtagtcgc cgggataggg cggcgtcggg
     4201 tcgcccgagg agagcttctc gaccgccgcg ggccacacgg gcgaggtaag cggcaccggg
     4261 tcggcgccga cgaacgagcc cgcgacgatc ccgccgtcgt ctgcgaaaag atcccagagt
     4321 ggatcggcgc ccgggccggc cggcgctacg ccgcggtcgg ccagcagcgc taggccggtc
     4381 gcgatgtgct cttcctggtc ggcgatgatc cgcttcagca tccgcacggt cggctcgtcg
     4441 acgtacggtg gacagccctc gaggtggcgg cggaggtccg caagcagcgc aggcttcacg
     4501 accccgaaga gggaggcaag gaagctttcg ggcccctccg cccgctcgag cgccacgagc
     4561 cccggcgccg accgtcgacg caggtgctcg aagtcggccg gccggatcat cagctccacg
     4621 agtcgcgctt cgagctcgtg cgcgtgatct gcatcctcgt agagcatcag gccgagcgcc
     4681 agtttctcgg gcagctcggg caccctcgag atccagccgc cgatgaggcg agagagctcg
     4741 acctcgacgg agtagaggac accgagccgg cgcgcgttcg cgcgcacgtc gtgcatgcgc
     4801 tcgggcgtca ctcgacggta gttcttgaca gcctggctca tctcgacctc cctgactcct
     4861 tgttcatcgg gcctcttccg gtggaggcac gatgacgacc ggacactgcg cgtgatgcgc
     4921 gcactgttga ctgaccgagc cgagcaggag ccctgtgaac ccgccgtgac cccgcgatcc
     4981 gacgaccagg agatcggcat ccgccgacgc ctcgatcaac tcgcgggcag cgttgccctc
     5041 tttgagcacg cgctccgcct cgacgccttc gagctccgcg agtgcgtcgt cgagcacgtg
     5101 ctcggcccga cgctggtagt cgtcgattac cccctccaga ggcggctgcg acaggacggg
     5161 gcgcccgagc acaccgggct cgggcagccc gaccacgagg gggacatgcc agacgtagac
     5221 gaccttgagc gcggctcccc ggtgctcggc ctcctcgacc gcccactgca gcgcggcctc
     5281 cgagcccggc gagccgtcga agccgaccac gatcgtctcg gagcccacga gggcgagtct
     5341 acgacgatcc gaggctcccg ggcggctcgg cctgcgtctc cgctgctatg ctccgcgccg
     5401 tacaagagag cgggtccggt ctgcgcctgg cgctttcctt acacgttcgt gaggttgtag
     5461 gggaggtgtt tttcatggct agcggaaccg tcaagtggtt caacgatgcg aaggggtacg
     5521 gcttcatcac ccctgacgag ggttcgaagg atcttttcgt ccatcacagc aacatcgctg
     5581 gcgagggctt caagtcgctc accgagggcg cccgggtgga gttcgagcag cgcgagggcg
     5641 aaaagggccc tgaggcgatt aacgtcgcaa ccgccgccta gcacggccgc taccgcccgt
     5701 gatcgctccg gcgatcacgg gcgctgcgat cttcgacgat cgcacgaagg gagagggcta
     5761 tcgccgagaa ggaagagaaa ctcgagatgg aaggcgagat catcgagtct ctgcgcaacc
     5821 ggatgttccg gatccgtctc gacaacggac acgagatcat cggttacacg tccggccgca
     5881 tgaagagatt ccgtatcagg atgatgctcg gagaccgcgt acgagtcgag gtctcgccgt
     5941 acgacctcaa ccgggggcgg atcgtctaca ggatgggtta ggcgccgggc ggcacgacgt
     6001 cgcgcgggcg cacctgtgga accctcccag aagggtcgga aacaccgtgg acataaaagt
     6061 gacgcttcgc accgggaacg agtacgtcct cgtccccggc aacagcgagc gcgacgtaga
     6121 ggacctgttt accgagctgg ccgcggggcg atctcaggcg cttcggggct gggtacccgt
     6181 cgcgtccgag aaaggcgatc ggatcgtcgt tcgcggggac gagatcgtgg agctccatct
     6241 cgtcggcggc gattgagcgt gtcgcgtggc aactgatgcc gtcggcgagg aagcgggaat
     6301 cgcccaccgc gtcgcctccc ggcgcgccaa cgagcggcgg gccgcagcca agaacccgcc
     6361 gaccgacggg cagggcctcg tcgagttcgc ctgcgagtgc atcacgaccg actgcgaacg
     6421 ctcggtgaag gttccgctgt acgtgtacga gcgcctcgtc gaatcaggcg accagtacct
     6481 cgtgcaggct gcccatcatg ccttcgcgcg tcatcgcacg atcgtgactc tcggcctgat
     6541 gagaatcgaa gagcgggcgt gagcgacgcg ctcgcggagc cgggaacggt ggactagctc
     6601 ctccgctcgt tcagccggca actcccagct gctgcatcat cgccccggtg tcggtgacgc
     6661 cccagtgctc gacgacgacg ccgtctcgga tgcgcaccca atcgatggtc gccacgtcga
     6721 tcttcttgcc cgtcgccggg atgtccatga acgtgccctg gtgcgtgccg cgcatgcgga
     6781 ggcgcacaac cgctgtgtcc tctgtcgtga tgatgtcctc cacctcgaag cgcatgtccg
     6841 ggaacgcgtc gagcatgatc ccgaagaact cctttatccc atctctcccc gctgcgagcc
     6901 ccggaaactc ctcgtgctcg acgaagtccg gcgagacgag ctcgtcgatg agctccagct
     6961 ttcgctcgtt gacgatctcg tcatagaaac ggcgcaccag cgcctcgtgc tcggttgcca
     7021 ttcgacaccc tcccgtcgtc ttcttgcggg cgaccttacg acgcacggcg acgtctgaca
     7081 agaaggcgga gagcgatgag gctcgcggcc agaccgcccg cgttctgtgc gcgccgccgt
     7141 gagaccacgc gtgtgtccgg ctttcgccta cgctcgcagc gggatgcccc acttcgaaaa
     7201 tctccctgcc gccgagttcg gcttccccgg cccgctgcgc gatcggctcg tcgccgcgat
     7261 cctcagcggc gacaagacgt ccacgaccgg gctgctcgag gagtaccggc gcgaggcaac
     7321 gcccgtcgag gaggtgggcc ttcgtgagcg cgtcgtcgac tcggagggcc gcagcgtcgc
     7381 cgtgatcgag acgaccgagg tagaggtgaa gcggctgggc gacgtcgacc tcacgttcgc
     7441 gatcgacgag ggtgaggggt tcgcgacgct cgacgagtgg cgcgcggccc acgtccgttt
     7501 cttcacgtcg gccgagatgc tggccgccct cggcgagccg ccggttccga tcgacgacga
     7561 cacgctcgtg gtctgcactc gcttccgcgt ggtcgagcgc ctctagcggc gcgggtcagc
     7621 gcgacgttgg cgtgcgcgca ggctcagcgc ggcgatccga ctcggcgttg actgccccgc
     7681 cgaggagcag agcaagcgcg ctgagccaga gccacgtgag caggatcacg acggcggcga
     7741 gcgagcccca ggccttgttg tacgacccga acctgctcac gtagagcgag aacagcccgg
     7801 aggcgagcag aaagacgatg acggtgatga ccgagcccag gctgaggaag cgccaacccg
     7861 agcgcccgtc cgtgttcggg ccgagagcca gtatccccgc gacggcagcg agcagccccg
     7921 cgatcaggat cggccactgc gcgctccacc agatccacgt gaccgcgctc tcgatcccca
     7981 gcgccgagcc gatccagcct gacaggtgag ggccgagaac gaggaagccg aaggcgagcg
     8041 cgaaggcgag gaacgcccag gcgagcatcg cgagaccgac cgcgcgctgt ttgacgaagc
     8101 ccctcgtctc ctctcgcgcg taaatgtggt tcagccggcg catgagcgcg ttcatcgcgc
     8161 cgatcgacgt ccagagcgcg agcagaaggc cgacgatgat catcagcaga ggccccgtct
     8221 ggctctccgt cgcccgtgtg aggctgtcgt cgatgagggc aatcgcctct gcgggcatga
     8281 ctccctcgag ccgttcgagg attctctcga tcgcactagg ccccgcggcc agactgaaga
     8341 tcccgacggc gacgaggagc accgcgggga tggcgagaaa cgtgtagtac gcgacggccg
     8401 ccgccgagtc ggtgacctcg tcggcgaacg cgcgtgtaac ggcccttcgg actagcccga
     8461 ccgacgcgcg cagactgccg ggcgggccgt caccgggcac gggctgaacc tgctcgtgcg
     8521 ccacgctgcg atcctagata cgacccgcgc ggcgaggccg gccgcggcct cgcgggtggt
     8581 tcagcgacga ctagggagtt tcgtccgcct ggtcgtcccc ttggccggcg acgtccttcg
     8641 ccttgtccac cgcctgctcg ccgacgtctt tggccgactc ggccacgtct cccgccacat
     8701 ccgtcgcctt gccggcggcg tcgccggccg cttcggcgcc cttgctgaac caacccttga
     8761 ttgtgtccgt caagcccatt ttgatctgct cgcctccacc cttcgcgggc gtcgctgaca
     8821 tcgagcgagg aagcatatac gtgacttttg cgcgacgtgt cacgagagtc ggctcgacca
     8881 ggaagtgtct actcggagga cgccgctctt tccgtcgcac ctcaggctcg tccctgcgtg
     8941 gaaagccgcc cgcccgctct cacccgacaa ggcggcgggc aacgttgaga gccgccacga
     9001 ccacgagccc tgttgcaacc gcaccgacga cggcagctgc gggcctccgc ttcgcgtcga
     9061 ttccctcgcg gaggtcggcg agcgcctgcg tcagctgctc gcgctcggcc gtgatgtcgc
     9121 gacggatctc ctccgtcgtg cggccctcat ccggcacggt tgcctctcag agcctgcttc
     9181 gtcagacgcg cctcctcgat cgcctgctct ggcttgagcg gcgtgccttc gcggaagaga
     9241 gctcgcgatg ccaatgcaag gccgaggacg aggagaagga gcaggacgaa cacgatcagg
     9301 agcgcaagcc accagtcgac gacgagggcg agcgccgcgg ccacagcagc gagcccgaag
     9361 cccacggcga agagcatgaa gatgccggcc gcgacggcca gagccgcgcc cgcacccaca
     9421 gtcgcaccct tgtgcttcag ctcctgccga gcaagctccc tctgcagagt cgccagcgac
     9481 ctcgcgtgct cgacagcgcg cttcgcggag gcacgaatcc cgcggggacg ggctgtcgat
     9541 ccgctctctc ccgcagccgt ggttgcgcgg tctgacgcat tttcgtcctg catggagtct
     9601 ctatcctgac acgcggcccg cgcagcggga tcatgaacgg aagagcggcg accgtcagcg
     9661 aacgtcgagg tacgtgacca tcccgaggtc ctcgtggctc gcgggtgtgc cgccgaagag
     9721 cgagcagtag agcttcaccg tcccggcgct cagctggacg gagagagttc gcgtctctcc
     9781 cggcggaacg gcaacgacct tgaggttgcc gcccgcgtcg acgacggcga ggtcgtggtc
     9841 gtccatgcca cggttgtaga cgttgaaggt gacctcgccc gcgccgaccg ccgggcggga
     9901 gagcgcgatg gcgaactcgg tctcgtcgac ggccacgatc cgcggcagcg gcggcggcgg
     9961 cgggggcggg ggcggcggag ccgaaggggg tggctgaggc gccggcgggc tcgcaggctg
    10021 cccagggctc ggcggcggag caggaggggg cggctgcggc t
//
LOCUS       DBNGNK010000330         4291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1992704,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000330 DBNGNK010000000
VERSION     DBNGNK010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1992704"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="AC150V_09750"
     CDS             complement(<1..233)
                     /locus_tag="AC150V_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR3763241.1"
                     /translation="MSARRVTICDVGPRDGLQNDAKVLEPGVRAELVDRLAACGLPRV
                     EAVSFVHPKRVPQMAGAEEVVAAIARRPGVVYAG"
     gene            complement(230..1480)
                     /locus_tag="AC150V_09755"
     CDS             complement(230..1480)
                     /locus_tag="AC150V_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR3763242.1"
                     /translation="MPASDGDRPAAVEEARRIGPLAGIRVIELGTLLAGPFTGRLLGD
                     LGAEIIKVEAPGKPDPIREWGKARYEGRSLWWPVQSRNKKCITLNLRVERGQELLLEL
                     VKRADVVTENFRPGTLERWNLGFERMREVNPGIILARVSGYGQTGPYSKRAGFASVSE
                     AMGGIRHISGFPDQPPPRMHISLGDSLAGMFTAQGVLAALYWRDATGSGEGQVVDVSL
                     MEASFALLESTVPEYDRLGIVRGPQGTNLKGVAPSNIFKSADDKWIVIAANADNVFGR
                     LCEAIGRPELAEDPKFATHLARGDNQEEIEGIIADWAAERDAKEIDDVLNEAGVICGP
                     IYTIADIFEDAHYKERDMLLEHDDPEFGSYIGPGIVPKLTETPGAVRWSAPWDEGSHN
                     AEIYGDLLGLSDDELAGLREERVV"
     gene            complement(1467..2186)
                     /locus_tag="AC150V_09760"
     CDS             complement(1467..2186)
                     /locus_tag="AC150V_09760"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR3763243.1"
                     /translation="MRKVFAVDAPLSRPASAVAASVIREAIVDGRLEPGARLKEENLA
                     TELGISRTPIREALLLLQSEGLVEAFPNRGAFVRSYEIDELRDLYELRALLEGHAARR
                     AAERISDERIEELRASCGRFDALVRRADPPNLETVQELVRENLLFHSTILTAADNERL
                     AGMVRQVVAVPLVYQSYIWYSPDQARASAHAHHQLVHAFEQRDAEGAELLMRVHVQGA
                     RDVLADHVATAAHAARSDAGV"
     gene            complement(2239..>4291)
                     /locus_tag="AC150V_09765"
     CDS             complement(2239..>4291)
                     /locus_tag="AC150V_09765"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3763244.1"
                     /translation="IPDSDAQRATDLLEERFPAKAGDQAQLVFSAPDGLAQPDATAAV
                     LAAQKEAAAIPGVTEVGDLYAEQNGSLSDDGSIGFANVQFDQQAWDVPRGDVEKLKEA
                     TLAATEGSPVALEFTGPVIQDPPESGSSEVLGLFAALVVLLVVFGTLVAALIPIGLSL
                     VAVGTGLSVLAIASAFTNFNDVTPILATMIGLGVGIDYALFIVTRFRQALHDGHDPVA
                     AATIAGATSGRAVIFAGTTVAISISGLAVIGLDFITKLGVGASITVLTSVLLAVTLLP
                     AILALLGHRVDRLKVPLTGRRDDSREALADSRAARWGRRVTGDPWTYAITVLGVLVLL
                     AIPALTLVQLGASDNGTAPRSTTVRRAYDLLADGFGAGFNGPLLVAVDVRGDSQAAEG
                     LRSELQGVPGVADVPEPAFNDARDTALITVYPETSPQSEETQKLVERLRSDAVPAALA
                     GTEAEAYVGGLTAAFDDIADRIFERFGIFLLTVIGITFLVLTMAFRSVVIALMASVTT
                     ILSVLAAGGLLVLVFQEGIGIEAIGLDRTGPIESFLPVIVFAILFGLSMDYMVFLGSR
                     IREDYVHGHAAHLAVRHGVATIGRVIMAAATIMAVVFFAFLLNADRVTKEFGLALGAA
                     ILIDAFLVRLTLVPAVLWLLGDKAWYMPGWLDRILPRLTIESPVEDDERSEERPEPAS
                     A"
BASE COUNT          690 a         1486 c         1464 g          651 t
ORIGIN      
        1 cccgcgtaca cgacgccggg tcgccgcgcg atcgccgcca cgacctcttc ggcgccggcc
       61 atttgcggca cgcgcttcgg gtggacgaag ctgaccgcct cgacccgggg gaggccacac
      121 gccgccagcc ggtcgacgag ctcggcgcgc acgcccggct cgagcacctt ggcgtcgttc
      181 tgcagcccgt cgcgcgggcc cacgtcgcag atcgtcaccc gccgcgcgct cacacgaccc
      241 gctcctcccg tagcccagcg agctcgtcgt cggagagccc gagcaggtcg ccgtagatct
      301 cggcgttgtg gcttccctcg tcccaaggcg ccgaccagcg gaccgcgccc ggggtctcgg
      361 tcagcttcgg cacgatcccg gggccgatgt agctgccgaa ctccgggtca tcgtgctcga
      421 ggagcatgtc gcgctccttg tagtgagcgt cctcgaagat gtcggcgatc gtgtagatcg
      481 ggccgcagat gacgcccgcc tcgttcagga cgtcgtcgat ctccttcgcg tcccgctcgg
      541 cggcccagtc cgcgatgatc ccctcgatct cctcctggtt gtcgccgcgg gccagatggg
      601 ttgcgaactt cggatcctcc gcgagctccg gccgaccgat cgcctcgcag aggcgcccga
      661 agacgttgtc cgcgttcgcg gcgatgacga tccacttgtc gtcggccgac ttgaagatgt
      721 tcgacggagc gacgcccttg aggttcgtgc cctgcgggcc ccgcacgatg ccgagccggt
      781 cgtactcggg cacagtgctc tcgagcagcg cgaaggatgc ctccatcagc gagacgtcca
      841 cgacctggcc ttcgccgctg cccgtcgcgt cccgccagta gagcgcggcg aggactccct
      901 gcgccgtgaa catccccgcg agcgagtcgc ccagggagat gtgcattctg ggcggcggct
      961 gatcgggaaa gccgctgatg tggcggatac cgcccatggc ttccgacaca gaggcgaagc
     1021 ctgcccgctt cgagtacggg cccgtctgtc cgtagccgga caccctggcg aggatgatcc
     1081 cggggttcac ctcacgcatc cgctcgaagc cgaggttcca gcgttccagc gtgccggggc
     1141 ggaagttctc cgtcacgacg tccgcccgct tgacgagctc gagcagcagc tcctgtcccc
     1201 gctcgacacg aaggttgagc gtgatgcact tcttgttgcg cgactgcacg ggccaccaga
     1261 gcgatcggcc ctcgtagcgg gccttgcccc attcgcggat cgggtcgggc tttccgggcg
     1321 cctccacctt gatgatctct gcgccgaggt cgccgaggag tcggcccgtg aacgggccgg
     1381 cgagcagcgt gccgagctcg atcaccctga tccccgccag ggggccgatc cggcgggcct
     1441 cttcgaccgc cgccgggcgg tcgccgtcag acgccggcat cgctgcgtgc tgcatgcgcc
     1501 gcggttgcca cgtgatccgc gaggacgtcg cgggcgccct ggacgtgaac ccgcatcagg
     1561 agctctgccc cctctgcgtc cctctgctcg aacgcgtgaa ccagctgatg gtgggcatgc
     1621 gcggaggccc tcgcctgatc gggcgagtac cagatgtacg actggtagac gagcggcacg
     1681 gcgacgacct gcctgaccat acccgccaac cgttcgttgt ccgcggcagt aaggatcgtc
     1741 gagtggaaca ggaggttctc gcggaccagc tcctgcaccg tttcgaggtt gggcggatcc
     1801 gccctccgaa cgagggcgtc gaagcgcccg cagctcgcgc ggagctcctc gatccgctcg
     1861 tcgctgatcc gctcggcggc tcgtcgcgct gcgtggccct ccagcagcgc ccggagctcg
     1921 tacaggtcgc ggagctcgtc gatctcgtat gaccgtacga acgcgccgcg attcgggaac
     1981 gcttcgacaa ggccctcgga ctgcagcagg agcagcgcct cacggatggg tgtccggctg
     2041 atccccagct cggtggccag gttctcctcc ttcaggcgcg cgcccggctc caggcgaccg
     2101 tcgacgatcg cctcccgaat gacggaggcg gcgacagcgc tggccggccg cgagagcggc
     2161 gcgtcgacgg caaagacctt ccgcacgcga tcgaacatac agtatgcaat ccgcggcgac
     2221 cagcccggaa ccgctgaatc aagcggatgc gggctctggc cgctcctcgc tgcgctcgtc
     2281 gtcctcgacc ggggactcga tcgtcaaccg cggcaggatg cggtcgagcc agcccggcat
     2341 gtaccacgcc ttgtcgccca gcagccagag caccgccggg acgagagtga gccggacgag
     2401 aaaggcgtcg atcaagatcg ccgcgcccag tgccagcccg aactccttgg tcacgcggtc
     2461 ggcattgaga aggaaagcga agaagacgac ggccatgatc gtggccgccg ccatgatcac
     2521 gcgacctatc gtggcaacgc cgtggcgtac ggcgaggtgg gcggcgtgtc cgtgcacata
     2581 gtcctccctg atccgcgagc cgagaaacac catgtagtcc atggagagcc cgaacagaat
     2641 cgcgaagacg atgacgggca agaagctctc gatcggtccg gtgcgatcga gtccgatcgc
     2701 ctcgatgccg atgccctcct ggaagacgag tacgagtaga ccgccggcgg cgaggacgga
     2761 gaggatcgtc gtcaccgacg ccatcagggc gatcacaacg gagcgaaacg ccatcgtcag
     2821 gacgagaaag gtgatcccga tcaccgtcag caggaagatg ccgaaccgct cgaagatccg
     2881 gtcggcgatg tcgtcgaacg ctgcggtgag gccgcccacg taggcctcgg cctccgtgcc
     2941 ggccagcgcc gctggaacgg cgtcggagcg aaggcgctcg acgagcttct gcgtctcctc
     3001 cgactgcggc gacgtctccg ggtacacggt gatcaacgca gtgtcccggg cgtcgttgaa
     3061 tgcgggctcg ggcacgtcgg ccacaccggg aacgccctgg agctcgcttc gcagcccctc
     3121 ggctgcctgt gagtcgccac gcacgtcgac tgcgacgagg agcgggccgt tgaagccggc
     3181 gccgaagccg tcggccagca ggtcgtaggc cctgcgcacg gtcgtcgacc tgggcgcggt
     3241 gccgttgtcg gacgccccga gctgcaccag cgtcagcgcc ggaatcgcga gcagtaccag
     3301 cacgcccagg actgtgatcg catacgtcca gggatcgccc gtgacgcggc ggccccagcg
     3361 ggccgcgcgg gagtccgcga gcgcctcccg tgagtcgtct cgcctccccg tcagcggcac
     3421 cttgagcctg tcgaccctgt gaccgagcag ggccagaatc gcgggaagga gggtgaccgc
     3481 cagaagaacc gacgtgagca cggtgatcga ggcgccgact ccgagcttcg tgatgaagtc
     3541 gagtccgatg acggcaagtc cggaaatcga gatcgcgacc gtcgtgccgg cgaagatgac
     3601 ggctcggccc gacgtcgctc cggcgatcgt cgcggcggcg accggatcgt ggccgtcgtg
     3661 cagggcctgc cggaagcggg tgacgatgaa gagcgcgtag tcgattccga cgcccagacc
     3721 gatcatcgtg gcgaggatcg gcgtcacgtc gttgaagttc gtgaacgccg aggcgatggc
     3781 gaggacagac aacccggtcc cgacggcgac gagggaaagg ccgatcggga tgagcgccgc
     3841 cacgagcgtc ccgaagacga cgagcagcac gacgagtgcc gcgaacagtc cgagtacctc
     3901 gctcgagccg ctttcgggcg gatcctggat caccggcccg gtgaactcga gcgcgacggg
     3961 agagccctcc gtggcggcaa gcgtcgcttc cttcaacttc tccacgtcgc cgcgtgggac
     4021 gtcccaggcc tgctggtcga actggacgtt cgcgaacccg atcgacccgt cgtcggagag
     4081 ggagccgttc tgctccgcat agaggtcgcc gacctccgtc actcctggga tcgcggcggc
     4141 ctccttctgt gccgcgagta ctgccgccgt cgcgtcgggc tgcgccagac cgtccggggc
     4201 ggagaacacg agctgcgcct ggtcgcccgc cttcgcgggg aagcgctcct cgaggaggtc
     4261 ggtggcgcgc tgcgcgtcgg agtcggggat c
//
LOCUS       DBNGNK010000331         4319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1346343,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000331 DBNGNK010000000
VERSION     DBNGNK010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1346343"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..332)
                     /locus_tag="AC150V_09770"
     CDS             complement(18..332)
                     /locus_tag="AC150V_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019590.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0000423 - mitophagy [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HIR3763245.1"
                     /translation="MIVEQRDYHVHTGKLPELMKLYETEGIPIQQEVLGGLIGAFTTE
                     VGGLSTYTHLWRYESYDDRAERRAALLARDDWKAFLAKIQPMIHTQHNRLLVPTAFSP
                     IK"
     gene            complement(329..1795)
                     /locus_tag="AC150V_09775"
     CDS             complement(329..1795)
                     /locus_tag="AC150V_09775"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR3763246.1"
                     /translation="MTAVESPLLHRQAYVAGRWVDADSGETFPVVDPATGETLADVPR
                     MGAGETRRAIEAADAARPGWSGLLAKDRARLLRELAETMLDRKDDLSALLTLEQGKPL
                     SESHAEIAYAASFLEWFGEEAKRVYGDTIPPPLPGRRILVLKQPVGVTAGITPWNFPA
                     AMITRKAAPALAAGCPMVVKPAEQTPLSALAIADLAEQVGFPPGVFSVVTGDAADAPV
                     IGGELTSNPTVRKLGFTGSTEVGKLLMAQAARTVKKISLELGGNAPFLVFDDADLDVV
                     MEAFATCKFRNSGQTCISANRVLVQRGVFDDVADRLSQMASGLKVAPGFDEGAQIGPL
                     IDSAGLEKVEQHVADAVDRGGEVVVGGSAHALGGTFFEPTVLTGVTADARMSCEETFG
                     PVVGMIPFESEEQAVELANDTPFGLASYFFTRDLARAWRVGEALETGIVALNTGFIST
                     EVAPFGGVKESGIGREGSKYGIEEWLELKYFTMAGVGG"
     gene            1822..2199
                     /locus_tag="AC150V_09780"
     CDS             1822..2199
                     /locus_tag="AC150V_09780"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3763247.1"
                     /translation="MEPQFIRFDDVRPFELIDGVTGRPLFGESAMINLIEFEAGSTVP
                     QHSHPHEQLGIVLRGMQALVVNGVARELGPMEGYVLPGGVEHSAYCGPEGATVVDIFQ
                     PVREDYREQWEAGAAREGEEKES"
     gene            complement(2269..2553)
                     /gene="clpS"
                     /locus_tag="AC150V_09785"
     CDS             complement(2269..2553)
                     /gene="clpS"
                     /locus_tag="AC150V_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR3763248.1"
                     /translation="MEAPIEVEEPELGDKWVEERPWVVIVWDDPINLMSYVTMVFQKL
                     FGYSQSKATRLMLQVHNEGKAVVANGTREKAESDVSRLHAHGLWATMQQD"
     gene            2605..3087
                     /locus_tag="AC150V_09790"
     CDS             2605..3087
                     /locus_tag="AC150V_09790"
                     /inference="COORDINATES: protein motif:HMM:NF020989.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HIR3763249.1"
                     /translation="MPLPRPRIRRTRSGEIRVRIPTRERAILRELPHQLRRVLDSDDP
                     DLERLFPPAYPDDPVSNADFDRLVRSELVSGKQRALRVFEETVDAEQLDEEQLEAWIG
                     ALNDLRLVLGTRLGVDESTYSVPIDPHGPRAQEHALFGYLTWLQEQAVQARLAAPTWE
                     "
     gene            3118..3678
                     /locus_tag="AC150V_09795"
     CDS             3118..3678
                     /locus_tag="AC150V_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR3763250.1"
                     /translation="MLWLWILLGVLAVLLVLFVLGFNRLVRLRNEVHTGWANIEVQLE
                     RRADLIPNLVETVKAYAAHERETFQRVTEARAALVRAASPRAAAEADDILTAALGRLF
                     AVAEAYPELKASENFLRLQEDLTDTEDKIAAARRYYNATVLRYNTGIQSLPWVLVAGA
                     LGFRAREFFSADEDARAVPKVELTGR"
     gene            3688..>4319
                     /locus_tag="AC150V_09800"
     CDS             3688..>4319
                     /locus_tag="AC150V_09800"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR3763251.1"
                     /translation="MTLQEQIRANRFRSVFTVAGFFVLVAIVAAIVGLTLDIAIGAFA
                     FAGAMIYAAFAILRSRSMVSAMTGAHPLPPDEQRQLRRLVENVSIAAGLPVNPELRIV
                     DDPAPNAFASGLRPEKSFVGVTTGLLNTMPKRELEAVIAHEVGHIRNRDTYLMTMAAV
                     FVGVIALLADIGFRALAWGGGRRRGPIGIIAAVLGVVLAPYAAMLLRLVLS"
BASE COUNT          672 a         1500 c         1424 g          723 t
ORIGIN      
        1 gcccatacgc cgcagcgcta tttgatcgga gagaacgccg tcgggacgag cagccgattg
       61 tgctgcgtgt ggatcatcgg ctggatcttg gccaggaagg ccttccagtc gtcccgtgcc
      121 agcaacgctg cgcgccgttc cgcgcggtcg tcgtagctct cgtagcgcca gaggtgcgtg
      181 taggtcgaca gccctcccac ctcggtcgtg aacgcgccga tcagaccgcc gaggacttct
      241 tgctggatcg gaatgccctc ggtctcgtag agcttcatca gctccggcag cttgccggtg
      301 tggacgtggt agtcgcgctg ctcgacgatc atccgccgac gcccgccatc gtgaagtact
      361 tcagctccag ccattcctcg atcccgtact tcgacccctc gcgcccgatg ccggactcct
      421 tcacgccgcc gaagggggcg acctcggtgg agatgaagcc ggtgttgaga gcgacgatgc
      481 cggtctcgag agcctcgccg acgcgccagg cgcgggcgag atcgcgggtg aagaagtagg
      541 acgcgagccc gaacggagtg tcgttcgcca gctcgacggc ctgctcctcg ctctcgaacg
      601 ggatcatccc cacgaccggc ccgaaggtct cctcgcagga catgcgcgca tccgcagtga
      661 cgcccgtcag cacggtcggc tcgaagaacg tcccgccgag cgcgtgcgcg gagcccccca
      721 cgaccacctc gccgccgcga tcgacggcgt cggcgacgtg ctgctcgacc ttctcgagcc
      781 cggccgagtc gatcagcgga ccgatctgcg ctccctcgtc gaaccccggc gcgaccttga
      841 gccccgacgc catctgggag agtcggtcgg cgacgtcatc gaacacgccg cgctgcacca
      901 gcacgcggtt cgccgagatg cacgtctgcc cggagttccg gaacttgcac gtcgcgaacg
      961 cctccatcac aacgtcgagg tcggcgtcgt cgaagacaag gaagggcgcg ttgccgccga
     1021 gctccagcga gatcttcttc acggtccgcg ccgcctgcgc catgagcagc ttcccgacct
     1081 ccgtggagcc ggtgaagccg agcttccgga cggttgggtt ggatgtgagc tcgccgccga
     1141 tcacgggcgc gtccgcagcg tcacccgtca cgacgctgaa gactccgggc gggaagccga
     1201 cttgctcggc gaggtcggcg atcgccagcg cggagagcgg tgtctgctcg gccggtttga
     1261 cgaccatggg acaacccgcc gccagggccg gcgccgcctt ccgcgtgatc atcgccgcgg
     1321 ggaaattcca cggcgtgatg ccggcggtga cgcctacggg ctgcttgagg acgagaatgc
     1381 gcctgccggg aagcggcggc gggatcgtgt ctccgtacac ccgcttcgct tcctcgccga
     1441 accactcgag gaatgacgca gcgtacgcga tctcggcgtg cgactcgctg aggggcttgc
     1501 cttgctcgag tgtgaggagc gccgagagat cgtccttccg atccagcatc gtctcggcca
     1561 gctcgcggag cagccgggcg cggtcttttg ccagcaaacc gctccagccg gggcgagcgg
     1621 cgtcggcggc ctcgatcgcc ctccgcgtct cgccggcgcc catccggggc acgtcggcca
     1681 acgtctctcc ggtcgctgga tcgacgacag ggaatgtctc acccgagtcg gcatcgaccc
     1741 agcgtcccgc gacgtaggct tggcggtgga gcagcggcga ttcgactgcc gtcacaggac
     1801 agacgataat ccaagtcggg catggagccg cagttcatcc gcttcgacga cgtccgcccg
     1861 ttcgagctca tcgacggtgt gaccgggcgc cctctcttcg gcgagtcggc gatgatcaac
     1921 ctgatcgagt tcgaggcggg gtcgaccgtg ccgcagcatt cccatccaca tgagcagctc
     1981 ggcatcgtgc tccgcggtat gcaggcgctc gtcgtgaacg gcgtcgcgcg cgagctcggc
     2041 cctatggagg ggtacgtcct ccccggcggc gtcgagcact ccgcctactg cgggccggag
     2101 ggggcgaccg tcgtcgacat cttccagccc gtccgcgagg actaccgcga gcagtgggag
     2161 gccggcgcgg cgcgtgaggg cgaggagaag gagtcgtagc ggcctgctac cagcagcccc
     2221 cgaaactcag tgcgaggccg cacgagcaag taacagactg ttacttgctc agtcctgctg
     2281 catcgtcgcc cacaggccgt gggcgtgcaa gcgcgacacg tcggactccg ccttctcccg
     2341 cgtcccgttc gcgacgaccg ccttgccctc gttgtgcacc tgcagcatca gccgggtcgc
     2401 cttcgactgc gagtagccga agagcttctg aaacaccatc gtcacgtagc tcatgaggtt
     2461 gatcgggtcg tcccagacga tgacgaccca gggtcgctcc tcgacccact tatcgcccag
     2521 ctcgggctcc tcgacctcga tcggtgcttc gatcgtcttc gtgctcaccc cgtaattgtg
     2581 acgcgcgcgt aacgttcgac gtcggtgccc ttgccaagac cccggatccg acggacgcgc
     2641 tccggcgaga tcagagtgcg gattcccacc cgggagcggg ccatcctgcg cgagctcccg
     2701 caccagctgc ggcgcgtgct cgattctgac gaccccgatc tggagaggct cttcccaccg
     2761 gcgtaccccg acgacccggt gagcaacgca gatttcgatc ggctcgtgcg aagcgagctc
     2821 gtctcgggca agcagcgggc cctgcgcgtc ttcgaggaga cggtcgacgc agagcagctc
     2881 gacgaggagc agctggaagc gtggatcggg gctctgaacg acctccgcct cgtactgggg
     2941 acgcggctgg gcgtcgacga gagcacgtac tcggtcccga tcgacccgca cggcccccgc
     3001 gcacaggagc acgctctctt cgggtacctc acgtggctcc aagagcaggc ggtgcaagct
     3061 cgtctagccg ctccgacgtg ggaatgaccc ggccgccccg ccgtctaggc tctcaccgtg
     3121 ctctggctct ggatcttgct cggtgtcctg gcagtcctgc tggtgctctt cgtgctcggg
     3181 ttcaaccgcc tcgtccggct ccggaacgag gttcatacag ggtgggccaa catcgaggtg
     3241 cagctggagc ggagggccga cctcattccg aacctcgtcg agactgtgaa ggcctacgcc
     3301 gcgcacgaac gggagacgtt ccagcgggtg acggaagcgc gggcggcgct ggtgcgcgca
     3361 gcctctcccc gagcggctgc tgaggccgat gacatcctga ccgcggccct tggccgcctt
     3421 ttcgcagtgg ccgaggccta cccggagcta aaagcgtccg agaacttcct ccgactgcag
     3481 gaagacctca ccgacacgga ggacaagatc gccgccgcgc ggcgctacta caacgcgacg
     3541 gtgctgcgct acaacaccgg gatccagagc ctcccctggg tgctcgtagc gggcgcgctc
     3601 ggcttccgcg ctcgcgagtt cttctcggcc gacgaggatg cacgcgcagt gcctaaggtc
     3661 gagctcaccg gccggtagct cagccccgtg acgctccaag agcagatccg agcgaaccgg
     3721 ttccgcagcg tgttcacggt ggcgggcttc ttcgtcctgg tcgcgatcgt ggctgcgatc
     3781 gtcggactca cgctcgacat cgcgatcggc gcgttcgcct tcgccggcgc catgatctac
     3841 gcggcgttcg cgattctccg gtcgcgcagc atggtctcgg cgatgacggg tgcgcatccg
     3901 cttcccccgg acgagcagcg gcagctgcga cgcctcgtcg agaatgtgtc gatcgccgcg
     3961 gggctgccgg tgaaccccga gctccgcatc gtcgacgacc ctgctccgaa cgcctttgcc
     4021 agcggcctcc ggcccgagaa gagctttgtc ggcgtgacga ccggcctgct caacacgatg
     4081 cccaagcggg agctcgaggc ggtgatcgca cacgaggtcg ggcatatccg caaccgagac
     4141 acgtatctga tgacgatggc cgcggtcttc gtgggcgtga tcgcgctgct cgcagacatc
     4201 ggcttccgcg ctctcgcctg gggtgggggc cgccggcgcg ggccgatcgg gatcatcgcc
     4261 gccgtgctcg gtgtcgttct cgcgccctat gcggcgatgc tgctacgact cgtcctctc
//
LOCUS       DBNGNK010000332         6916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1777245,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000332 DBNGNK010000000
VERSION     DBNGNK010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6916
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1777245"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            607..2613
                     /gene="tkt"
                     /locus_tag="AC150V_09805"
     CDS             607..2613
                     /gene="tkt"
                     /locus_tag="AC150V_09805"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR3763252.1"
                     /translation="MSSDPGRLCIDTIRALAMDAVQKASAGHPGTAMALAPVAYLLYR
                     DVMRHNPGNPAWPGRDRFVLSAGHACVLQYTTLHLAGYDVTMDDLQHFRQWGSRTPGH
                     PELGHTPGIETTTGPLGQGFANGVGLAVAQRFLAERYNRPQHEIVDHRIYAICSDGDL
                     MEGITNEAASIAGHLGLGSLVYVYDDNHITIDGSTTLTFTTEDKGARFESYGWHVLHV
                     DDSEDLDALRGALDAAAAETQRPSLIVLRSHIAYPAPHAIDTAKAHGAPLGEDEVRRT
                     KEAMGFDPDVSFAVADDVRAHMAAVVERGAELERDWSDRFAAWSAAYPELANEWELDR
                     ARVPREGWLDALPEFPAGESVATRDAGKTVMSWLRDYTPTMIGGAADLVESTKTEFSG
                     GGVFARAHAGRNVPFGIREHGMGGIVNGIALEPAMLKPYGSTFLIFSDYMRPSVRLSA
                     LSELPVVWVWTHDSVGLGEDGPTHQPVEHHMALRAIPNLWYVRPGDANETAMAWRIAL
                     ERPDGPVAMALTRQKVPTLDRTEVQPAEGALRGAYTLGQRDEGDPDLILLATGSEVSI
                     ALEAARLVDANARVVSMPCWELFELQTADYRDEVLPPPVRARLSVEAGIGLGWERWVG
                     DHGDSVSIERFGASAPGERIFEELGFTASAVAERAAAVLDRVAA"
     gene            2673..3776
                     /gene="tal"
                     /locus_tag="AC150V_09810"
     CDS             2673..3776
                     /gene="tal"
                     /locus_tag="AC150V_09810"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR3763253.1"
                     /translation="MAQSRLHQLLAAGVSPWIDSLSREMLETGELERLMREDAIVGVT
                     SNPTIFQQALAEGDWYDEQLRQLLGTQDDPKEIFFALAREDIRDACDLMRPVWQSSDG
                     VDGYVSLEVDPGLAYEREATFDQAMRLHSEVDRPNLLVKIPATELGLGAIEDCIAAGK
                     SINVTLIFSLERYAAVVEAYLRGLERLVAHGGDPAPVRSVASFFVSRVDTEADRRLEE
                     VGNTELQGRLAIANARLAYEHFLGAFAGTRWDYLAGKGARPQRCLWASTSTKTPAYSD
                     VLYVEELIGPDTVNTMPAVTVAAFQDHGVVADTLREGVDEARELLADLAAAGVDYEDV
                     VAVLEAEGVQKFSDSFDSLLEGIAAKRQALTAA"
     gene            3773..5269
                     /gene="zwf"
                     /locus_tag="AC150V_09815"
     CDS             3773..5269
                     /gene="zwf"
                     /locus_tag="AC150V_09815"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR3763254.1"
                     /translation="MSNPLLEGLELGRRPDPCVIVVFGASGDLTKRKLFPALYALAYR
                     NLLPERFAVVAAARSEGTTRKFLAEMKRVVKEFGRDPFRAEVWERLAAGTHYVTADLA
                     ADGGEDALIATLARLDDERGTAGNRLAYLAVPPQAFEAVVRKVGENRSPDGWTRVIVE
                     KPFGTDLESARQLNELLDKWFSEGEIFRIDHYLGKETVQNMLALRFANGIFEPVWNRS
                     FVDHVQISVSESMGIEGRAAFYEQAGAVRDIFQNHLLQLVALTAMEPPIDFSAESVRN
                     EKVKVLRSLRTPAPRAVVRGQYGRGFVDGEEVPAYREEEGVAQGSVTETYVAGRLHVD
                     SWRWAGTPFYFRAGKRLAKRETSIAIQFQRAPHPPFASIAGEGLRPNVLLIKLQPNEG
                     ISLAIGAKVPGAGMTIRTVHLDFLYGGAFRTGLPEAYERLILDAMLGDATLFTRADEV
                     EEQWKLVDGMIAGWQRDRPSFPNYPAGTWGPATADELVHADGRSWRRH"
     gene            5275..6123
                     /locus_tag="AC150V_09820"
     CDS             5275..6123
                     /locus_tag="AC150V_09820"
                     /inference="COORDINATES: protein motif:HMM:NF021632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HIR3763255.1"
                     /translation="MGVVADVERALWQLRCRESGDDAFAPELRASTMTHVVWAPPDWL
                     ETAQATFAGLHERHPARTIFLVPQGGRGECEASAALEAAAVDELSREIYAEVIEIHLR
                     GVAARHPASVVRPLLLPDLPAFCRWRGEPRWTGGAFDEVVGVVDRLIVDSGEWRGLPA
                     SYGRLAARFDGPAVSDVAFARLHPWRVKLAQRWPGIRRAERLRVQGPRAEALLLAGWL
                     RSRLRRDVALTRRNADTVEAVWIDGEEVEPPSEPPGVAAELLSAELDVFGRDPVYEAA
                     ALRAAA"
     gene            complement(6120..6866)
                     /locus_tag="AC150V_09825"
     CDS             complement(6120..6866)
                     /locus_tag="AC150V_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR3763256.1"
                     /translation="MGPDLAFALELADAADTISLRRFRAGDLVIETKPDLTPVTDADR
                     AVEEAIRRRLAAERPDDGVLGEEYGDDGPSTRRWIIDPIDGTRNYSRGLPVWGTLIAL
                     EVDSAPVVGVVSAPALERRWWAERDGGAFAAGDSIQVSRVSRVEDAVVCLPAEHPLPL
                     LSERAWLPRGYGDFWAHMLVAEGAADGAIDAVGVHPWDLAPVQVIVEEAGGRYSDASG
                     ERRIDTRTGLTSNGLIHDELVAAAASLPGA"
BASE COUNT         1064 a         2369 c         2449 g         1034 t
ORIGIN      
        1 cgccgcaatc ccggtcacag acggtgagca ggtggatcgc ctttggcgaa caccacgacc
       61 tgagagaagc cgctcgtctg atcggcagcc tcggccctgg tcagacgcgg gcgaacctcg
      121 ccgtcggcac gacagccgga cgaagttcaa gcgagtgccg tccgtgaacg gcgtctacgg
      181 tgcctgcttc aatctcgaca ccggcgcgct ccgtctcatt cccgtgaacc tcgggtgcgt
      241 cgcgacgacc gagcggcgcg catcgtggtc gaagagcggc ccacagggca ggcgcggtcc
      301 ggagggtagg ccaggtccgc aaggcaaggc cggcccacag ggtgctcccg gcccacaggg
      361 cgctcccggc cccatcggtc aggttcagag gtcgggccgc aggcccagcc gacaagcagg
      421 gtgatcaggg acctgatcgg cggcgaaagg tcgcgcttcg agcctccttc ccgagcggcc
      481 ccggctcttg gaccgcagag gcgatcgtca cagccagcat cagcggcggc cgcaaggcca
      541 ccgtcagtgc ctacgcggtc tgcagcgcgt agcgcgtaga ggggccggcc cgttagggtt
      601 cgccgcgtga gcagcgaccc cggccgtcta tgcatcgaca cgatccgcgc cctcgcgatg
      661 gacgccgtcc agaaggcgag cgcgggccat cccgggacgg cgatggcgct cgcacccgtc
      721 gcatatctgc tctatcgcga cgtcatgcgg cacaaccccg gcaatcccgc ctggcccggc
      781 cgggaccgct tcgtcctgtc cgccggccac gcctgcgtgc tccagtacac gaccctgcac
      841 ctcgccggct acgacgtcac gatggacgac cttcagcact tccgccagtg gggctcccgc
      901 acgcccggcc atcccgagct cggtcacacg ccggggatcg agacgacgac aggcccgctc
      961 gggcagggct tcgcgaacgg cgtgggcctc gccgtcgccc agcgcttcct cgctgagcgc
     1021 tacaaccggc cgcaacacga gatcgtcgac catcggatct acgccatctg ctcggacggc
     1081 gacctgatgg aggggatcac caacgaggcg gcctcgatcg cgggccatct cggtctcggc
     1141 agtctcgtgt acgtgtacga cgacaaccac atcaccatcg acggctccac cacgctgacg
     1201 ttcaccaccg aggacaaggg agcccggttc gagtcgtacg gctggcacgt gctgcacgtc
     1261 gacgactcgg aagacctcga cgctttgcgc ggggcgctcg acgcggccgc ggcggagaca
     1321 cagcgcccgt cgctgatcgt cctccgcagc cacatcgcgt acccggcgcc gcacgcgatc
     1381 gacacggcca aggcgcacgg agctcccttg ggcgaggacg aggtgcggcg aacaaaggag
     1441 gcgatggggt tcgatcccga cgtgagcttc gccgtcgccg acgacgtccg agctcacatg
     1501 gcggctgtcg tcgagcgggg cgcggagctc gagcgggact ggagcgaccg ctttgcagcc
     1561 tggagcgccg cctaccccga gctcgcgaac gagtgggagc tcgaccgggc gcgagtgccc
     1621 cgcgagggct ggctcgacgc gctgccggag ttccccgccg gggagtcggt ggcgacgcgt
     1681 gacgcgggca agaccgtgat gagctggctc cgcgactaca cgccgacgat gatcggcggc
     1741 gccgccgatc tggtcgagtc gacgaagacc gagttcagcg ggggcggcgt cttcgcacga
     1801 gcccacgccg gccggaacgt ccccttcggc atccgggagc acgggatggg gggaatcgtc
     1861 aacggcatcg cgttggagcc ggcgatgctc aagccgtacg gctcgacgtt cttgatcttc
     1921 tccgactaca tgcggccctc cgtgcggctg tcggcgttgt cggaactgcc cgtggtctgg
     1981 gtctggacgc acgactcggt cgggctggga gaggacgggc cgactcatca gcccgtcgag
     2041 caccacatgg cactgcgcgc gatcccgaat ctctggtacg tacgccctgg cgacgcgaac
     2101 gagacggcga tggcttggcg gattgcactc gagcggcccg acggccccgt tgcaatggca
     2161 ctcacgcggc agaaggtgcc gacgctcgac cgcacagagg ttcagcccgc ggagggtgcg
     2221 ctccgcggcg catacaccct tgggcagcga gacgagggag accccgacct gattctgctg
     2281 gccacgggat cggaggtgtc gatcgcgctc gaagccgcgc gcctcgtcga cgccaacgca
     2341 cgtgtcgtgt cgatgccctg ctgggagctc ttcgagcttc agaccgccga ttaccgcgac
     2401 gaggtgttgc ccccgcccgt tcgggcgcga ctgtcggtcg aggcgggcat cgggctcggc
     2461 tgggagcgct gggtgggcga ccacggtgac tcggtctcga tcgagcgctt cggggcctcc
     2521 gcgccgggcg agcgcatctt cgaggagctc ggcttcacgg cgagtgcggt cgccgagcgt
     2581 gcagctgccg tactcgaccg cgtcgcggca tgatgtcgct gaacacgaga ccatgagcgc
     2641 ggccggtccg gtcgcgccga aaggctggtg acatggccca atcgagactg caccaactgc
     2701 tcgcggcggg cgtcagcccg tggatcgact cgctctcgcg cgagatgctc gagaccggag
     2761 agctggagcg cctcatgcgc gaagacgcga tcgtgggcgt cacgtccaac ccgacgatct
     2821 tccagcaggc gctcgcggag ggagactggt acgacgagca gctccggcaa ctgctcggga
     2881 cgcaggacga cccgaaggag atcttctttg ctctggcgcg cgaggacatc cgcgacgcct
     2941 gtgacctgat gcggcccgtc tggcagtcct cggacggcgt cgacggctac gtctcgctgg
     3001 aggtcgatcc cggtctcgcg tacgagcggg aggccacctt cgaccaggcg atgcggctcc
     3061 actccgaggt cgatcgtccg aacctcctcg tgaagatccc ggcaacggag ctcgggctcg
     3121 gggcgatcga ggactgcatt gcagcaggga agtcgatcaa cgtgacgctg atcttctcgc
     3181 tcgagcgcta cgcggcggtc gtggaagcct acttgcgcgg cctcgagcgt ctcgttgccc
     3241 acggcggcga cccggcgccg gttcgctcgg tggcaagctt cttcgtatcg cgcgtcgaca
     3301 ccgaggccga tcgccgcctg gaggaggtcg gcaacacgga gctgcagggg cgcctcgcca
     3361 tcgcaaacgc aagactcgcg tacgagcact tcctcggcgc cttcgccggg acgcgttggg
     3421 actacctggc cggcaaggga gcccggccgc agcggtgcct ctgggcatcg acctcgacca
     3481 agacccccgc ctacagcgac gtcctctacg tcgaggagct catcgggccc gacaccgtca
     3541 acacgatgcc ggccgtcaca gtcgcggctt tccaggacca cggcgtcgtc gcggacaccc
     3601 tgagagaggg tgtcgacgag gcgcgcgagc ttctcgccga cctcgcagcg gcgggagtcg
     3661 actacgagga cgtcgtcgcc gtgctcgagg ccgaaggcgt gcagaagttc tccgactcgt
     3721 tcgactcgct gctcgaggga attgccgcca agcggcaggc gctcacggcc gcgtgagcaa
     3781 cccgctgctg gaggggctgg agctcgggcg gcgtcccgat ccctgcgtga tcgtggtctt
     3841 cggtgcgtcc ggcgacctca ccaagcgaaa gctcttcccg gcgttgtacg cgctcgcgta
     3901 ccgcaacctg ctgccggagc gcttcgctgt cgtcgcagcc gcgcggagcg aaggaaccac
     3961 gcggaagttc ctcgccgaga tgaagcgcgt cgtcaaggag ttcggccgtg atccgttccg
     4021 tgccgaggtg tgggagcgcc tcgccgccgg cacgcactac gtcacggcgg atctggccgc
     4081 cgacggcggc gaagacgcgc tcatagcgac gcttgccagg ctcgacgacg agcgcggcac
     4141 ggccggaaac cgtctcgcct acctcgccgt tccgccgcag gcgttcgagg ccgtcgtccg
     4201 caaggtcggc gagaaccgca gtcccgacgg ctggacgagg gtgatcgtcg agaagccgtt
     4261 cggcaccgac ctcgagtccg cccgccagct gaacgagctg ctcgacaagt ggttctcgga
     4321 gggcgagatc ttccggatcg accattacct gggcaaggag acggtgcaga acatgcttgc
     4381 cctgcggttc gcgaacggca tcttcgagcc ggtctggaac cgctccttcg tcgaccacgt
     4441 gcagatcagc gtgtccgagt cgatggggat cgagggccgc gcggcgttct acgagcaggc
     4501 gggggcggtt cgcgacatct tccagaacca cctgctgcag ctggtcgcgc tgacggcgat
     4561 ggagcctccg atcgacttct ccgccgagtc ggtgcggaac gagaaggtga aggtgcttcg
     4621 gtcgctgcgc acgccggcgc cgcgggcagt cgtccgcggt cagtacgggc ggggctttgt
     4681 cgacggggaa gaggttcccg cataccggga ggaggagggc gtcgcgcagg gctcggtgac
     4741 ggagacgtac gttgcgggcc ggctgcacgt cgacagctgg cgctgggcgg gcaccccgtt
     4801 ctacttccgt gccggcaagc ggctggccaa acgcgagacg tcgatcgcga tccagttcca
     4861 gcgggcgccg catcctccgt tcgcttcgat cgccggagag ggcctgcgcc cgaacgtcct
     4921 gctgatcaag cttcagccga acgaggggat ctcgctggca atcggcgcga aagtgcccgg
     4981 agccggcatg acgatcagaa ccgtgcatct ggacttcctc tacggcggcg ccttccggac
     5041 cggcctgccg gaggcgtacg agcggctcat tctcgatgcc atgctcggcg acgcgaccct
     5101 cttcacccgt gcggacgagg tcgaggagca gtggaagctg gtcgacggga tgatcgcagg
     5161 ctggcagcgc gaccgcccga gctttccgaa ctatccggcg gggacgtggg ggccggcgac
     5221 cgccgacgag ctggtgcacg cggacggccg ctcctggcgg cgtcactgag cccgatggga
     5281 gtcgtcgccg atgtcgaacg cgctctgtgg cagctccgct gccgggaaag cggcgatgac
     5341 gcctttgcac ccgagctgcg cgccagcacg atgacacacg tcgtttgggc gccccccgat
     5401 tggctggaga cggcgcaggc gaccttcgcg ggactgcacg agcggcatcc ggcgcgcacg
     5461 atcttcctgg tgccgcaggg gggccggggt gagtgcgagg caagcgcagc tctcgaggcg
     5521 gccgccgtcg acgagctctc ccgtgagatc tacgcggagg tgatcgagat ccacctgcgt
     5581 ggggtcgccg ccaggcaccc ggcgtcggtc gtccggcccc tcctgctccc cgacctcccg
     5641 gcattctgcc gctggcgtgg cgagccccgc tggacaggcg gcgcgttcga cgaggtcgtc
     5701 ggggtcgtcg accgcctgat cgtcgactcg ggtgagtggc gcgggctgcc ggcgtcctac
     5761 ggtcgcctcg ccgcgcggtt cgacggccct gccgtctcgg acgtcgcctt cgccaggctc
     5821 cacccgtggc gcgtcaagct ggcccagcgc tggccgggta tccggcgggc agagcgactg
     5881 cgcgtccagg gcccgcgtgc ggaggcgctc ctcctggcgg gatggcttcg cagccgtctg
     5941 cgccgagacg tcgcgctgac gcggcggaac gccgataccg tcgaggcggt ctggatcgac
     6001 ggagaagagg tcgagccacc gtcggagccg ccgggcgttg cggccgagct cctgtcggcc
     6061 gagctcgacg tcttcgggcg tgatcccgtg tacgaagccg cagcgctgag agcagccgcc
     6121 taggcgccgg gaagtgaggc ggctgcggcg acgagctcgt cgtggatcag cccgttcgag
     6181 gtgagcccgg tgcgcgtgtc gatgcgccgc tcgcctgacg cgtcgctgta gcggccgccg
     6241 gcctcctcga cgatcacctg gacgggtgcg aggtcccagg gatggacgcc gacagcgtcg
     6301 atcgcaccat cggccgctcc ctccgcgacg agcatgtggg cccagaagtc gccgtagcct
     6361 cgcggcagcc aggcccgctc cgagaggagc ggaagcggat gctcggcggg caggcagacg
     6421 actgcgtctt cgactcggct caccctcgac acctggatcg agtcgcccgc agcgaacgcc
     6481 ccgccgtctc gctctgccca ccagcgccgt tcgagagcag gggccgacac gactcccacg
     6541 accggggcgg aatcaacctc gagcgcgatc agcgttcccc atacagggag cccacgcgag
     6601 tagtttcgtg tcccgtcgat ggggtcgatg atccatctgc gtgtcgacgg gccgtcgtca
     6661 ccgtactcct ctcccagcac gccgtcgtcc ggtcgctccg cagccagccg tcggcggatc
     6721 gcctcctcca ctgcccggtc tgcgtcggtg accggagtca ggtcgggttt ggtctcgatg
     6781 acgaggtcgc ccgcacggaa gcggcggagc gagatggtgt cggccgcgtc ggcgagctcc
     6841 agtgcgaagg cgaggtcggg ccccacgcgg caagcgtaga tggtcgttct acgaggagac
     6901 ggccggtcgg ccgggg
//
LOCUS       DBNGNK010000333         5233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_774072,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000333 DBNGNK010000000
VERSION     DBNGNK010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_774072"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="AC150V_09830"
     CDS             <1..485
                     /locus_tag="AC150V_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3763257.1"
                     /translation="GGGQSGTNLTALADVPLENFFAAGVQNRVVFVLTDGESAPIDEA
                     AIARAYGRQPKPTLVLVHVGGPGERVFDPEGRPEPAYTPVEQRAATPRRIASITGARL
                     FGEGEGAAAADHALAAIGAGGPTEQQARGLERRPLGAWALAAAVLPLAYLLRRRNADR
                     "
     gene            531..604
                     /locus_tag="AC150V_09835"
     tRNA            531..604
                     /locus_tag="AC150V_09835"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:565..567,aa:Met,seq:cat)
     gene            640..801
                     /gene="rpmG"
                     /locus_tag="AC150V_09840"
     CDS             640..801
                     /gene="rpmG"
                     /locus_tag="AC150V_09840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR3763258.1"
                     /translation="MATGVRVAITLACTECKRRNYQTQKSKRNTPDRVEFNKYCRWCG
                     RHTAHRETR"
     gene            807..1106
                     /gene="secE"
                     /locus_tag="AC150V_09845"
     CDS             807..1106
                     /gene="secE"
                     /locus_tag="AC150V_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR3763259.1"
                     /translation="MAKESRRQRRDARRAAGDDAVSASPAGQAAVEAPAAQSRRRGGF
                     IRESAAELQKVDWPNRNQVIQGTIVVIIACAVVGGYLWGVDQALRPFVREILLGQ"
     gene            1121..1654
                     /gene="nusG"
                     /locus_tag="AC150V_09850"
     CDS             1121..1654
                     /gene="nusG"
                     /locus_tag="AC150V_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR3763260.1"
                     /translation="MFNWYVINTYSGHENKVKANLEHRIESMNQTQRFRRVVVPTEQV
                     IETKDGQKVQAEKRVLPGYVLVNMDLNDDAWIVVKGTPGVTGFVGAGAKPVPLSQGEV
                     ERILQTGAAAGAPAPKAQVEFTLGESVKVTSGPLSDFDGEIVDVNADQQKLKVLVDIF
                     ERQVPVELGFDQVKKID"
     gene            1673..2098
                     /gene="rplK"
                     /locus_tag="AC150V_09855"
     CDS             1673..2098
                     /gene="rplK"
                     /locus_tag="AC150V_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR3763261.1"
                     /translation="MAKKVLTLIKLQIPGGQANPAPPVGPALGQHGVNIMEFTKAFNA
                     QTAHENGRIIPVEITVFEDRSFTFITKTPPAAVLIKEALGIEKGSGEPNIEKVGRLSQ
                     GQLRSIAEAKMPDLNARDVEQAMKVVAGTARSMGVEVDE"
     gene            2095..2826
                     /gene="rplA"
                     /locus_tag="AC150V_09860"
     CDS             2095..2826
                     /gene="rplA"
                     /locus_tag="AC150V_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR3763262.1"
                     /translation="MKRGKRYANSRAKIDRERVYSPVEAVRVLKSFGDTKFDETVEVH
                     FRLGLNVRHADEQLRGTLMLPHGTGQDVRIAVFAEGDKAREAEEAGADIIGSADLAQK
                     IEEGFLEFDVAIATPDQMGNVGKLGRVLGPRGLMPNPKTGTVTFEVAKAVQDAKAGKL
                     EYRTDRGANIHVAIGKKSFDEQKLVENYAALLDEIVRARPASAKGRYIRGITLTTTMG
                     PGIRIDPTRTKDITAEQEQEEAVPA"
     gene            2999..3796
                     /gene="rplJ"
                     /locus_tag="AC150V_09865"
     CDS             2999..3796
                     /gene="rplJ"
                     /locus_tag="AC150V_09865"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR3763263.1"
                     /translation="MLRADKERIVAELEEKLQATESLIVADYRGLSNAELADLRTKLL
                     ENGARFTVVKNSLARRAAETAGADDLLALIDGPTAIAFVEASGNTAAVAKVLVDTATQ
                     SKILAVRGGIMSGAPISEAEVESLAKLPPVDVLKGQLVGAIVAPLTQLAALLAAPLRD
                     LVGLVDARIAQLEEEGEGVETPAADAEPEDVAAEVAGAEEPAGEAAAADEQPAAETTS
                     AEEAAAGEAAPDEASEGGETEPEPAEPAAEADNTDTENTETDSNEEE"
     gene            3802..4191
                     /gene="rplL"
                     /locus_tag="AC150V_09870"
     CDS             3802..4191
                     /gene="rplL"
                     /locus_tag="AC150V_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018947939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR3763264.1"
                     /translation="MATDVKGVLDSLGNMTVLELVELKNAIEEEWGVTAAAPVAVAAP
                     GAAVPGGDGGAVAEEKDAFDVVLAETGGQKIAVIKVVRAATGLGLKEAKDIVDGAPNT
                     IKEGLPKEEADKLKGELEEAGATVELK"
     gene            4395..>5233
                     /locus_tag="AC150V_09875"
     CDS             4395..>5233
                     /locus_tag="AC150V_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR3763265.1"
                     /translation="MSSTAVAPRARKSFSRLKHVLDLPNLIDIQKASFRWFLEEGLRE
                     TIDDISPIEDYTGTLAVEFGDYEFGEPQFTIQECREKDLTYQAPLSMTVRFVNTETGE
                     IREQRVFMGDFPMMTEWGTFIINGTERVIVTQLVRSPGAYLMEPKDAAKQVFTANLMP
                     SRGSWLELEIDKKGIVYARIDRKRKLPITTLLRALPAEDPTTGFQLDTSSNEKILQLF
                     PSGTSYIARTLEKDPSTREEEALIEVFKKQRPGEPPTLDNARNLLRALFFDPKRYDLT
                     KVGR"
BASE COUNT          997 a         1736 c         1787 g          713 t
ORIGIN      
        1 gtggcggcgg ccagtccggc accaacctga ccgcgctcgc ggacgtcccg ctcgagaact
       61 tcttcgccgc gggtgttcag aaccgcgtgg tgttcgtgct caccgacggg gagagcgctc
      121 cgatcgacga agcggccatc gcccgcgcct acggcaggca gccgaagccg acgctcgtcc
      181 tcgttcacgt cggcgggccc ggcgagcgcg tgttcgaccc ggagggcagg cccgagccgg
      241 cgtatacgcc ggtcgagcag cgagcggcga cgccgcggcg aatcgcttcg atcaccggcg
      301 cccggctctt cggcgagggc gagggagccg ccgcggccga ccacgcgctc gcggcaatcg
      361 gcgcaggcgg accgaccgag cagcaggccc gcggcctcga aagacgccca ctgggcgcct
      421 gggccctggc cgccgccgtc ctgcccctcg cctacctgct gcgccggcga aatgcagatc
      481 gctgacggag gccgacgacg gaagaaccct tgctacactg cgacccgcgc ggcggcgtag
      541 ctcagttggt tagagcagcg gaatcataat ccgcgtgtcg taggttcgag tcctaccgcc
      601 gctactcgca cccttcgtcc gaacgagcac tcaagaacaa tggccacagg agtcagagtc
      661 gcaattacgc tcgcctgcac ggagtgcaag cgacgcaatt atcagaccca gaagtcgaag
      721 cggaacacgc ccgaccgggt cgagttcaac aagtactgcc gctggtgcgg tcgtcacacg
      781 gcgcatcggg agacgcgcta ggcggcatgg cgaaggaatc ccgcaggcag cgccgcgatg
      841 cgagacgtgc ggcgggcgac gacgccgtga gcgcgtcgcc ggccggccag gctgccgtcg
      901 aggcgccggc cgcccagagc cgccgacgcg gcggcttcat ccgggagtcc gccgccgagc
      961 tgcagaaggt cgactggccg aaccgcaacc aggtgatcca gggaacgatc gtcgtgatca
     1021 tcgcctgcgc ggtcgtgggc ggctatctct ggggtgtcga ccaggcgctc cggccgttcg
     1081 tgcgtgagat cctgctaggg caataggcga aggcgtcgcg atgttcaact ggtacgtgat
     1141 caacacctac tcggggcacg agaacaaggt caaggccaac ctcgagcacc gcatcgagtc
     1201 gatgaaccag acgcagcgct tccgtcgcgt cgtcgtcccc accgagcagg tcatcgagac
     1261 gaaggacggg cagaaggtgc aggccgagaa gcgtgtgttg ccgggctacg tcctcgtcaa
     1321 catggacctg aacgacgacg cctggatcgt cgtcaagggg acgcccggcg tgacggggtt
     1381 cgtcggcgca ggggccaagc ccgttccgct ttcgcaaggc gaggtcgagc ggatcctcca
     1441 gaccggagcc gccgccggcg ctcccgcgcc gaaggcgcag gtcgagttca ccctcggcga
     1501 gtcggtgaag gtaacctccg gcccgctctc cgacttcgac ggcgagatcg tggacgtcaa
     1561 cgccgatcaa cagaagctga aggtgcttgt cgacatcttc gagcggcagg ttcccgtcga
     1621 gctcggcttc gaccaggtga agaagatcga ctaggacggt tcgaccagcc agatggccaa
     1681 gaaggtactc acgctgataa agctccagat ccccggcggg caggcgaacc ctgcccctcc
     1741 ggtcggcccc gcgctcggac agcacggggt gaacatcatg gagttcacga aggccttcaa
     1801 cgcgcaaacg gcgcacgaga acggccgcat cattcctgtg gagatcacgg ttttcgagga
     1861 tcgctcgttc acgttcatca cgaagacccc gccggccgcg gtgctgatca aagaggcgct
     1921 cgggatcgag aagggctccg gcgagcccaa catcgagaag gtcgggaggc tgtcccaggg
     1981 acagctccga tccatcgccg aggccaagat gccggatctg aacgcacgcg acgtcgagca
     2041 ggcgatgaag gttgtcgcgg gaaccgcccg ctcgatggga gtggaggtgg acgagtgaag
     2101 cgcggcaagc gctacgcgaa ctcgcgggcg aagatcgacc gcgagcgtgt ctacagcccc
     2161 gtcgaggcgg tgcgcgtcct gaagagcttc ggcgacacga agttcgacga gaccgtcgaa
     2221 gtgcatttcc ggctcggact caacgtccgc catgcggacg agcagctgcg cggcacgctg
     2281 atgctgccgc acgggaccgg ccaggacgtc cgcatcgcag tgttcgccga gggcgacaag
     2341 gcgcgtgagg cggaagaggc aggcgccgac atcatcggct cagccgacct cgcacagaag
     2401 atcgaggagg gcttcctcga gttcgacgtc gccatcgcga cgcccgatca gatgggcaac
     2461 gtaggtaagc tcgggcgggt tctcggcccc cgggggctga tgccgaaccc caagacgggc
     2521 accgtgacct tcgaggtcgc gaaggcggtg caggacgcga aggccggcaa gctcgagtac
     2581 cgcaccgacc gcggcgccaa catccacgtt gcgatcggga agaagagctt cgacgagcag
     2641 aagctcgtcg agaactacgc ggcgctgctc gacgagatcg tgcgggcccg gccggcttcg
     2701 gcgaagggcc gctacatccg cggcatcacg ctgacgacga ccatggggcc cgggattcgc
     2761 atcgacccca cgcggacgaa ggacatcact gcggagcaag agcaagagga ggcggttccc
     2821 gcctagagaa gcaccggcaa cagcccgtcg ccacagacag ccggcggcca gcgcgtagcg
     2881 cattgctacg tgccggcaga agaccggccg aggcggcctc gacggtgaac aggcgtccgc
     2941 gccttccctc gagcccgcct gcgtgcgggc tcgaagcacg aaaggaggtg gagtaaggat
     3001 gctacgagct gacaaggaac gaatcgtggc cgagctggag gagaagctcc aggccacgga
     3061 gtccctcatc gtcgcggact accgcgggct gtcgaacgcc gagctcgccg acctgcgcac
     3121 gaaactgctc gagaacggcg cgcgcttcac tgtcgtcaag aactcgcttg cgcgacgtgc
     3181 tgcagagacg gccggcgcgg acgacctgct tgcactgata gacgggccga ccgcgatcgc
     3241 cttcgtcgag gcgagcggaa ataccgcggc cgtcgccaaa gtgctcgtcg acacggcgac
     3301 ccagtcgaag atcctcgccg ttcgcggtgg gatcatgtcc ggcgccccga tcagcgaggc
     3361 cgaggtcgag agcttggcga agctgccgcc cgtcgacgtg ctcaaggggc agctcgtggg
     3421 cgcgatcgtg gctccgctca cccagctggc ggccttgctc gcagcgccac tgcgtgatct
     3481 ggttgggctg gtcgacgccc ggatcgccca gctcgaggag gagggcgagg gtgtggagac
     3541 tccggcggcg gacgccgagc cggaggacgt cgccgccgaa gtggccggag ccgaagagcc
     3601 cgccggcgaa gcggccgccg ccgacgagca gcccgccgcc gagacgacct ccgcagagga
     3661 ggcggcagcg ggcgaggcgg caccggacga ggcatccgag ggcggggaaa ccgagccgga
     3721 gcctgccgag ccggctgcgg aagccgacaa caccgacacc gagaacaccg agaccgattc
     3781 gaacgaggag gagtaagcga catggcaacc gatgtgaagg gcgtcctcga ctcgctcggg
     3841 aacatgaccg ttctcgagct tgtcgagctc aagaacgcga tcgaggagga gtggggcgtc
     3901 acggcggccg ccccggtggc cgttgctgcc ccgggcgcgg ccgtgccggg tggtgacggc
     3961 ggcgccgtgg ccgaggagaa ggacgccttc gacgtcgtcc tggccgagac cggcgggcag
     4021 aagatcgccg tgatcaaagt cgtccgtgcc gccaccggcc tcgggctcaa ggaggccaag
     4081 gacatcgtcg acggcgctcc caacacgatc aaggaagggc ttcccaagga agaggccgac
     4141 aagctcaagg gcgagctcga ggaggcggga gccaccgtcg agctcaagta agccgctgac
     4201 gccgcccgcc cggcggaggg tctcgacgcc ctccgccggc gcccgctgct acccttaggc
     4261 gcacgaagcg acgtccgcag ccgcccgcgg ttgcgcgacg tcatctccgt gcgctatcct
     4321 tcggggttcc gcgagctccg ctcctataac tctgcccgct ccaccaatcg cacgccgcta
     4381 agggaggctg acacttgagc agcaccgctg tcgcgcctcg cgcgcgcaag agcttttcgc
     4441 gcctgaagca cgtcctcgac ctccccaacc tcatcgacat ccagaaggcc tcgttccggt
     4501 ggttcctgga ggagggcctg cgagagacga tcgacgacat ctcgccgatc gaggactaca
     4561 caggcacgct cgccgtcgag ttcggtgact acgagttcgg cgagccgcag ttcaccattc
     4621 aggagtgccg tgagaaggac ttgacctatc aggcccctct ctccatgacc gtccggttcg
     4681 tcaacaccga gaccggagag atccgcgaac agcgggtctt catgggcgac tttccgatga
     4741 tgaccgagtg ggggacgttc atcatcaacg gcaccgagcg cgtcatcgtg acgcagctcg
     4801 tccgcagccc cggcgcttac ctgatggagc cgaaggatgc ggcaaagcag gttttcacag
     4861 ccaacttgat gccgtcccgc ggctcgtggc tcgagctgga gatcgacaag aagggcatcg
     4921 tctacgcgcg gatcgaccgg aagcgcaagc tcccgatcac gacgcttctc cgcgcgctgc
     4981 ccgccgagga tcccacgacg ggcttccagc tcgacacgag ctcgaacgag aagatcctcc
     5041 agctcttccc ctcgggcacg tcctacatcg cccggacgct cgagaaggat ccgtcgaccc
     5101 gcgaagagga agctctgatc gaggtcttca agaagcagcg tcccggtgag ccgccgacgc
     5161 tggacaacgc tcggaacctc ctgcgcgccc tcttcttcga tccgaagcgc tacgacctga
     5221 ccaaggtcgg gcg
//
LOCUS       DBNGNK010000334         6506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1061824,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000334 DBNGNK010000000
VERSION     DBNGNK010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1061824"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="AC150V_09880"
     CDS             <1..665
                     /locus_tag="AC150V_09880"
                     /inference="COORDINATES: protein motif:HMM:NF039581.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3763266.1"
                     /translation="LATDMVYEYVTTTYQRASQLDAAALTASFAELEGEARAQLEADD
                     VPADRMLIQRIAECRYLGQGYELRVDVGPGEIDEAWVDKMRADFHDIHEREYSRRFED
                     SDIEIPNIRVRGIGLMPALETPEVEVGGEAPDEALSREGEAWFRVDGTLAKVPTRYYN
                     RAELKAGNRIDGPAIVLQYDSTTVIPPGISARVDGFGNIIIEVGASDEARALAESMEV
                     RA"
     gene            662..2575
                     /locus_tag="AC150V_09885"
     CDS             662..2575
                     /locus_tag="AC150V_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007256169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3763267.1"
                     /translation="MSVMEYEVGRKPGPPTRKRIEVDPITLRVLGGAFHAIAKEMAGV
                     LFRMSYSSIIRESEDLGAGIFDAEGRELCESDSTPMHIGSLPWYIRGFLHTLGGTDAI
                     EEGDVIVHNHPYLGASHTPDIAVAVPIFHEDELLGFAAVTAHVLDVGGSYPGINADAF
                     DVYAEAKLYNGLRWYRRGELNKDLDRMIFENVRTETMNRGDMNAMLAACELGRDRMLR
                     LVQRYGVETVMSAGYDWMDYSERMLRTEIEKIPDGEYGPVVGWLDDDARNRGQQLRVE
                     TKVIVEGDEITIDLTGSNAEVPTGFNVPFEGSLLVGAYYAVRTILLDEVMFPEHVPQN
                     DGVFRPVQVIAPKGSIFNPSFPRACFSRFTQVQRVVDNTILALADALPDKVTAGNSAG
                     IHFCAYSGFDETAGEYWLYLEVNEGSYGGRYGKDAMDSVDNLMANTRNNPIEELDMRF
                     PVRCDQYELRPEPAAPGRWRGGIGIIRRNRFLVDGVYSCEGDRQTDAPLGIFNGWDGL
                     VASCRKNPDTDGQEMLDAKVTGVPFAAGEFIEFREPNAAGYGDPLDRPAEQVREDVLD
                     DFTTVELARDAYGVVFADETTLEIDEEATERLRDELRKSPNGNSLTGYFEGRYPVENK
                     AVSVAGNQEFGIT"
     gene            2582..3394
                     /locus_tag="AC150V_09890"
     CDS             2582..3394
                     /locus_tag="AC150V_09890"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3763268.1"
                     /translation="MTDTGEESAHPKGREPSLEGRLRPIERPASTVPVRRVRKSYEQV
                     ADQLRELILTGQLEQGTRLPNETILAGDFGVSRATVREALRLLAAQNLIRTAKGAGGG
                     SYVTLPTVDHISEFLRANIGLLTDARNLTLDELIEARALLEVPAARLAARRRQEEDVV
                     RLESSIPADSLRLDTQSEFVQNAEFHTILIECCGNRLLYIAAQPLFSALQTSLARSRL
                     GRTFHTGVHAQHALIAEAIGAGDEDAAASEMTSHLEFLVPFYEKAWRDLRRR"
     gene            3370..3948
                     /locus_tag="AC150V_09895"
     CDS             3370..3948
                     /locus_tag="AC150V_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HIR3763269.1"
                     /translation="MARPEATVMAVPAPTGMIHGRFQPFHNGHLEYLRGAAALSVRLL
                     VGITNPDRARTKTESPAPGREADDANPFTYTERAEMITGVLRDEGLVDVLVIPFPVSQ
                     PDLWPDYVPDGTVHFLRVYDAWGEVKRSRLRAAGYEVVVLDPGTQKSLDGTEVRRLLA
                     AGRDWSALVPPATARVISPLLGSSAEDLRRPA"
     gene            3920..5209
                     /locus_tag="AC150V_09900"
     CDS             3920..5209
                     /locus_tag="AC150V_09900"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BPG-independent phosphoglycerate mutase family
                     protein"
                     /protein_id="HIR3763270.1"
                     /translation="MRKICVVLLDGLGDRAYPELGGRSANEAASTPVLDTLCARGSCG
                     LLWPLGPGRAPATEVAHWSMLGYAEDDFPGRAVLEAVGRGIAVRGDAVYAFAALRPGV
                     VRDGCIWLTGRAGAEDVADCLELWPADEEVAGIGFRCHPAGNSEAIVELVGPSADDRL
                     TDTEPFFHDRDPLLRPLALDDTAANAAAAVETWSREVNAGLREHRVNERRRADGRAEL
                     AAVALKWWGRPRAVPAFAQLHGLSGTVGAASPMFRGLATTLALDFIELPETGNPGADT
                     SARLAAAAERMERGATFVWLHLKAIDEAGHTNDPGERVRVLEAIDSALEPLLEPPFSD
                     AVVCVTGDHATPAVPNVIHTGDPVPVALAAPSVRADRVESFGELECAGGLLGRLRGAD
                     LMPLLLNAADRALYTGSRPTPIRGATGHPAAPEALRA"
     gene            5209..6168
                     /locus_tag="AC150V_09905"
     CDS             5209..6168
                     /locus_tag="AC150V_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR3763271.1"
                     /translation="MRVSFGIQGSGQLTDGVPDPGRYRAVAELAEELGYDSIWAGEHL
                     SFHNPILDIGVALTAFAAWTSRVRLGAGILLLPLRHPSLVAKQAASIDYLSGGRLLLG
                     VGVGGEGEKDFEAAGVPIAERGRRADESIAALRALFGPPPATFSGRFYSFAGVESAPP
                     ADQPQGPPSIVGGSSPAAQRRAGRLGDGWLPYMLSARRYGEGLRAVREHARESGRDPD
                     ALLPGVVLFALVGDDGDTTRADAREHLSRRYGMKFEPHHIEKLCIAGTPDECRARLDE
                     YVEAGVRELCFNPCAAGEEFLDQCRRLHAEVVEPTRRERGWTT"
     gene            6156..>6506
                     /locus_tag="AC150V_09910"
     CDS             6156..>6506
                     /locus_tag="AC150V_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3763272.1"
                     /translation="MDDVKPPKRLVPVLRRIERLYEREVRPREEALMHRLTNSREYLD
                     ADGKLAPELWEARREIMRASAEAGVYSLHLPKEIGGGGLGRHDMIYAEEQVYSYGVYL
                     NPALLAWSEGATPRL"
BASE COUNT         1046 a         2248 c         2261 g          951 t
ORIGIN      
        1 tgctcgcgac ggacatggtc tacgagtacg tgacgaccac gtatcagcgc gcctcccagc
       61 tggacgcggc ggcgctgacc gcctccttcg cggagctcga gggagaggcc agggcgcagc
      121 tggaagccga cgacgtcccc gccgaccgga tgctgatcca gcggatcgcc gagtgccgct
      181 acctcggcca gggctacgag ctgcgggtcg atgtcgggcc gggcgagatc gacgaagcct
      241 gggtcgacaa gatgcgagcc gacttccacg acatccacga gcgtgagtac tcgcgccggt
      301 tcgaggactc ggacatcgag atcccgaaca tccgcgtgcg cggcatcgga ttgatgccgg
      361 cgctcgagac acccgaggtc gaagtgggcg gagaggcgcc agacgaggcc ctcagccgtg
      421 aaggcgaagc ctggttccgc gtcgacggca cgctggcgaa ggtgccgacc cgctactaca
      481 accgggccga gctgaaagcc ggcaaccgta tcgacgggcc cgcgatcgtc ctccagtacg
      541 actcgacgac ggtgattccg ccggggatct cggcgcgggt cgacggtttc gggaacatca
      601 tcatcgaagt gggcgcgtcg gacgaggcgc gcgcactggc cgagagcatg gaggtgcggg
      661 catgagcgtc atggagtacg aggtcgggcg caagcctggg cccccgacgc ggaagcgcat
      721 cgaggtcgac ccgatcacgc ttcgcgtgct cggcggcgcc ttccacgcga tcgccaagga
      781 gatggcgggc gtcctcttcc gcatgtccta ttcgtcgatc atccgtgagt cggaggatct
      841 gggtgccggc atcttcgacg ccgaggggcg cgagctctgc gagtccgact cgacaccgat
      901 gcacatcggc tcgctgccct ggtacatccg tggcttcctg cacacgctcg gcggaacgga
      961 cgcgatcgag gagggggacg tgatcgtcca caaccacccc tacctgggcg cgtcgcacac
     1021 ccccgacatc gccgtcgcgg tgcccatctt ccacgaggac gagctgctcg gcttcgccgc
     1081 cgtcacggcg cacgtcctcg acgtcggcgg ctcgtacccg ggaatcaacg ccgacgcatt
     1141 cgacgtctat gccgaggcga agctctacaa cgggctgcgc tggtatcggc gcggcgagtt
     1201 gaacaaggat ctcgaccgga tgatcttcga gaacgtccgt accgagacga tgaaccgtgg
     1261 cgacatgaac gcgatgctcg ccgcatgcga gctggggcgg gaccggatgc tacggctcgt
     1321 gcagcgctac ggcgtcgaga ccgtgatgag cgcgggctac gactggatgg actactcgga
     1381 gcgaatgctg cggacggaga tcgagaagat cccggacggc gagtacgggc cggtcgtcgg
     1441 ctggctcgac gacgacgccc gcaaccgcgg acagcagctt cgcgtcgaga ccaaggtgat
     1501 cgtcgaaggc gacgagatca cgatcgacct gaccggatcg aacgcagagg tgccgaccgg
     1561 cttcaacgtg ccgttcgagg gctcgctcct cgtcggagcg tattacgcgg tacgaacgat
     1621 ccttctcgac gaggtgatgt ttcccgagca cgtgccgcag aacgacggcg tctttcgccc
     1681 cgtccaggtg atcgcgccga aaggatcgat cttcaacccg agttttccgc gcgcctgctt
     1741 ctcgcgcttc acccaggttc agcgtgtggt cgacaacacc atcctcgcgc tcgccgatgc
     1801 gctgccggac aaggtcaccg cgggcaactc tgcgggcatc cacttctgcg cctactcggg
     1861 cttcgacgag accgcggggg agtactggct ctacctcgag gtgaacgagg gctcgtacgg
     1921 cggccgctac ggcaaggacg cgatggactc cgtcgacaac ctgatggcga acacgcgcaa
     1981 caaccccatc gaggagctcg acatgcgctt cccggtgcgc tgcgaccagt acgagctgcg
     2041 gccggagccg gcggcgccgg gacggtggcg cggcgggatc gggatcatcc gtcgaaaccg
     2101 cttcctcgtc gacggcgtct actcgtgcga gggcgaccgc cagaccgacg cgccactcgg
     2161 gatcttcaac ggctgggacg ggctcgttgc gtcctgtcgg aagaacccgg acacagacgg
     2221 gcaggagatg ctcgacgcca aggtgacggg cgtgccgttc gcggcaggag agttcatcga
     2281 gttccgcgag ccgaacgcgg cgggctacgg cgacccactc gacagaccgg ccgagcaggt
     2341 gcgcgaggac gtgctcgacg acttcacgac agtcgagctg gcccgggacg cttacggggt
     2401 cgtcttcgcc gacgagacga cgttggaaat cgacgaggaa gcgacggaac gactgcgtga
     2461 cgagctacgc aagagcccga atggaaactc gttgaccggc tacttcgagg gccgctatcc
     2521 ggtcgagaac aaggccgtct cggtggccgg caaccaggag ttcggcatca cgtagcccgc
     2581 cgtgactgac accggcgagg aaagcgcgca cccgaagggc cgcgagccca gtctcgaggg
     2641 ccgcctgcgc ccgatcgagc gccccgcatc gacggtaccg gtccgccgag tccgcaagag
     2701 ctacgagcag gtcgcagacc agctccgcga gctgattctc acgggccagc tcgaacaggg
     2761 aacccggctc ccgaacgaga ccatcctcgc gggcgacttc ggcgtcagcc gcgcgaccgt
     2821 gcgggaggcg ttgcggctgc tggcggcgca gaacctgatc cgcactgcga agggagcggg
     2881 cggcggcagc tacgtcacgc tcccgacggt cgaccatatc tccgagttcc tccgcgcgaa
     2941 catcggcctg ctcaccgatg cccggaacct caccctggac gagctgatcg aggcacgggc
     3001 cctgctcgag gttccggcgg cccggctcgc agcgcggcgg agacaggagg aagacgtcgt
     3061 gcgcctggag agctcgatcc cggccgactc gctgcgcctc gacacgcagt ccgagttcgt
     3121 ccagaacgcc gagttccaca cgatcctgat cgagtgctgc ggcaaccggc tgctctacat
     3181 cgccgcgcag ccgctgttct cggcactgca gacgagcctc gcgcggtcgc ggctcggccg
     3241 cacgttccac accggcgtgc atgcacagca tgccctcatc gccgaggcga tcggcgcggg
     3301 cgacgaagac gcggctgcga gcgagatgac gagccacctc gagtttctcg tccccttcta
     3361 cgagaaggcg tggcgcgacc tgaggcgacg gtgatggccg tgcctgcccc aacagggatg
     3421 atccacggcc gtttccagcc gttccacaac ggtcatctcg agtacctgcg cggcgcggcc
     3481 gcactgtccg tacgcctcct ggtcgggatc acgaacccgg accgggccag gacgaagacg
     3541 gagtcgcccg ctccggggcg cgaggccgac gacgcaaacc cgttcaccta cacggagcgc
     3601 gccgagatga tcacgggcgt cctgcgcgac gagggcctcg tggacgtgct cgtcattccc
     3661 tttcccgtct cgcaacccga cctgtggccg gactacgtgc cggacggcac ggtgcacttc
     3721 ctgcgcgtgt acgacgcgtg gggtgaggtc aagcgttcgc gactccgggc cgccggttac
     3781 gaggtcgtcg tcctcgaccc cggcacccag aagtccctcg acgggacgga agtgcggcgc
     3841 ctgctcgcgg cgggtcgcga ctggagcgcg ctcgtccctc ctgcgaccgc ccgggtgatc
     3901 tcccctctcc tcggctccag tgcggaagat ctgcgtcgtc ctgcttgacg ggctcggcga
     3961 ccgcgcctac ccggagctcg gcgggcgcag cgcgaacgag gccgcctcga caccggtgct
     4021 cgacactctc tgcgcacggg gctcctgcgg tctgctctgg cccctcggcc cgggacgagc
     4081 acccgcaacc gaggtcgccc actggtcgat gctcggctac gccgaggacg acttccccgg
     4141 acgagccgtg ctcgaggcgg tgggacgagg cattgcagtg cgcggcgacg ccgtgtatgc
     4201 gttcgctgct ctccgtcccg gcgtcgtccg cgacggctgc atctggctca cgggccgagc
     4261 gggcgcagaa gacgtcgccg actgcctgga gctctggcct gcggacgagg aggtcgcggg
     4321 catcggtttc cgatgccacc cggccggcaa cagcgaggcg atcgtcgagc tcgtcgggcc
     4381 gtctgccgac gatcgcctga cggacacgga gcccttcttc cacgaccgcg atccgttgct
     4441 caggccgctt gcgctcgacg acaccgcggc gaacgcggcg gcggcggtcg agacatggag
     4501 ccgcgaagtc aacgcgggac tccgtgagca ccgcgtgaac gagcgacgcc gtgccgacgg
     4561 ccgagcggag ctcgcggccg tcgcgctgaa gtggtggggc cgacctaggg ccgtgcccgc
     4621 gttcgcgcag ctccatggcc tttcgggcac cgttggggcg gcgtcaccca tgttccgcgg
     4681 gctggcgacg acgctcgcac tcgatttcat cgagctccct gagacgggca atccgggtgc
     4741 ggacacgagc gcacggcttg ccgcggcggc cgaacgcatg gagcgcggag ccacgttcgt
     4801 ctggctgcat ctcaaggcga tcgacgaggc ggggcacacg aacgaccccg gcgagcgtgt
     4861 ccgcgtgctc gaggcgatcg actcggcgct cgagccgctc ctggagccgc cgttctcgga
     4921 tgccgtcgtc tgcgtcaccg gagaccacgc gacgcccgcg gtgccgaacg tgatccacac
     4981 cggcgacccg gtccccgttg cgctggccgc gccgagcgtg cgcgccgatc gagtcgagtc
     5041 cttcggcgag ctggaatgtg caggcgggct cctcggccgg ctccgcggcg ccgacctcat
     5101 gccgcttctg ctcaacgccg ccgaccgggc tctgtacacg gggtcgcgtc ccactccgat
     5161 ccgcggagcg accggccatc cggcggcgcc ggaggcgctc cgggcgtgat gcgcgtctcg
     5221 ttcggcatcc agggcagcgg ccagctgacg gacggcgtgc ctgatcctgg ccggtaccgc
     5281 gccgtagccg agctcgccga ggaactcggc tacgactcga tctgggcggg cgagcatctg
     5341 tcgttccaca acccgatcct cgacatcggc gtcgcactga ccgccttcgc ggcctggacg
     5401 agccgtgtcc ggctcggagc cggcatcttg ctgctgccgc tccgccaccc gagcctcgtc
     5461 gccaagcagg ccgcttcgat cgactacctc tccggcggcc ggctcctgct cggcgtcgga
     5521 gtgggcggcg agggcgaaaa ggacttcgag gccgccggag tcccgatcgc cgaacgcggc
     5581 cgccgcgccg acgagtcgat cgcggcgctt cgcgcgctgt tcggaccccc acccgccacg
     5641 ttctccggcc gcttctacag cttcgccggc gtcgagagcg cgccgccggc cgaccagccg
     5701 caggggccgc cgagcatcgt cggcggctcg tcacccgcag cgcagcgtcg ggcggggagg
     5761 ctgggcgacg gctggcttcc ctacatgctc tcggcacgac gctacggcga ggggcttcgg
     5821 gcggtgcgcg agcatgcgcg ggagtcgggc cgcgacccgg acgccctgct gcccggcgtc
     5881 gttctcttcg ccctcgtcgg cgacgacggc gacacgaccc gcgcggacgc ccgcgagcac
     5941 ctttcgcggc gctacggaat gaagttcgag ccacatcaca tcgagaagct ctgcatcgcg
     6001 ggcacgccgg acgagtgtcg cgcgcgactg gacgaatacg tggaggccgg cgtgcgcgag
     6061 ctgtgcttca acccctgcgc ggcaggtgag gagttcctcg accagtgccg ccgcctgcac
     6121 gccgaggtgg tcgagccgac gagaagggag cgtggatgga cgacgtgaag ccgccgaagc
     6181 ggctcgtgcc ggtcttgcgg cgaatcgagc gcctgtacga acgcgaggtt cgcccccgcg
     6241 aggaggcgct catgcaccgg ttgacgaaca gccgcgagta cctggacgcc gacggcaagc
     6301 tcgctcccga gctctgggag gcgcgccgcg agatcatgcg cgcatcggcc gaggccggtg
     6361 tctacagcct gcatttgccg aaagagatcg gcggcggcgg actgggccgg cacgacatga
     6421 tctacgccga ggagcaggtc tactcgtacg gcgtgtatct caaccccgca ctgctcgcgt
     6481 ggtccgaggg cgcgacgcct cggttg
//
LOCUS       DBNGNK010000335         3881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1274480,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000335 DBNGNK010000000
VERSION     DBNGNK010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1274480"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..567)
                     /locus_tag="AC150V_09915"
     CDS             complement(67..567)
                     /locus_tag="AC150V_09915"
                     /inference="COORDINATES: protein motif:HMM:NF025698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HIR3763273.1"
                     /translation="MKRDFVQAAHPYEDTQVIDARAPRTNQAVIGSLALIAVTVGPWW
                     LLALLALQLAVGLTLGRRYCLPCLLYFEVIQPRFGEGPLEDARPPRFANVVGVVFLTA
                     ASLSYAAGWATLGAVLGGLVAALALLAALTGFCAGCNAYKVGFVVTGRRFVSCPLPPR
                     PQSEAA"
     gene            630..1565
                     /locus_tag="AC150V_09920"
     CDS             630..1565
                     /locus_tag="AC150V_09920"
                     /inference="COORDINATES: protein motif:HMM:NF025229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HIR3763274.1"
                     /translation="MARYASAALVAALLLATGAAFAYTEQLKLTRSPILGTSVTKLFS
                     PVCECPTDVATISFRLRDEDRLTVEMIDRTGTPVRELVRRQAEPAGAVDLIWDGRDDA
                     GVVVPEGAYRPRVRLNDDRRTITLPNPIRVDVTPPRFERFSIRPRVFSPDGDGRNDRV
                     VARYRVSERARVSLFVDGVRRVLKRGSRRDGAISWFGRIGGESVAPGPYVLTLGARDL
                     AGNTSRRTAPRTVAARYVALGRKVIETRPNARFAVLVASDARRIRWQLGQRTGTGRPG
                     TLRLRAPSRPGRYTLTVTASGNSVRAAVFVRDLAG"
     gene            1562..3088
                     /locus_tag="AC150V_09925"
     CDS             1562..3088
                     /locus_tag="AC150V_09925"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR3763275.1"
                     /translation="MSPAEISALIGCGGLALLVLAPRRDLRVVGLVAWAAGLAGLVVY
                     LAPDTSTTRLAAAAVGGLVLAGAGGWLLNRFPYLLAFATLACLPARLPIKLGDEEANL
                     LVPLYGVVAAYAVALGWQLVVRKDDRSRELGPIAWPLALFVAWAGVAMLWADDLREGS
                     IFVGAFLLPFALLAVGFSRLPWRGRWLTWLWGALVATALAYATVGMFQWATRDVFWNP
                     KVIVGNAYLPFFRVNSIFWDPSIYGRYLAVGILTALAGLLLGGIRGKKLLGLYAVIGA
                     LWVGLFFTFSQSSYVALAVGVVVAAVVVWRWRAVVGATILVVLVVAGTLALPDVRDRL
                     IDEGRSGVNAVTSGRASLVGQGLRIAVGDPVGGVGTGGFKRAYADRVGLVGEDPKRAA
                     SHTTPVTVAAENGVVGLAFFAWLLAAALFATLRGLGSGFTSRVSFAVGLTLLAITVHS
                     LFYNAFFGDPMTWALFGLVGLAASVPRRATAEETSRAVPVPEPEPVREPQPAATGGAP
                     "
     gene            3085..3720
                     /locus_tag="AC150V_09930"
     CDS             3085..3720
                     /locus_tag="AC150V_09930"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3763276.1"
                     /translation="MIGGSKRALVLAPHTDDGEFGCGGTMARLVDEGCEVRYVAFSIA
                     TRSLPAGFPPDTLAQEVRGATAELGIPESALTVHDFDVRTFPEHRQDILELLVELWEE
                     WRPDVVFQPSHHDVHQDHQTIAQEGLRAFKRTTILGYEIPWNNYDFAYGCYVALAESH
                     IERKVAAVQRYASQQHRRYANADYIWNLARVHGTNVNREYAEVFQVYRIVA"
BASE COUNT          521 a         1380 c         1338 g          642 t
ORIGIN      
        1 gtgctcctca cgctgcgggg ccggtgcggc gggcgagcct tcgctcaccg cgccattatc
       61 cccgggttag gcggcttcgc tctgcggccg cggcggcagc gggcagctca cgaagcgccg
      121 gcccgtgacg acgaagccga ctttgtatgc gttgcacccg gcgcagaagc cggtgagcgc
      181 cgcgagcagg gcgagggctg cgacgagacc cccgagcacg gcgcccaagg tcgcccagcc
      241 agccgcgtac gagagggacg ccgcggtcaa gaacacgact cccacgacgt ttgcgaagcg
      301 cggcggccgg gcgtcctcga gcggtccctc gccgaagcga ggctggatca cctcgaagta
      361 cagaaggcag ggtaggcagt aacggcggcc gagcgtgagc cccacggcca gctgcagcgc
      421 gagcagggcg aggagccacc acggcccgac cgtcaccgcg atcagcgcga gcgagccgat
      481 caccgcctgg ttcgtgcgcg gggcgcgtgc gtcgatcacc tgcgtgtcct cgtaggggtg
      541 agcggcttgg acgaagtctc gcttcatttc gggaagtatt agcacactca gccattccgg
      601 acccgtcggg cggcttacaa tcgccggacg tggcccggta cgcgtcagcc gccctcgtcg
      661 cggcgctgct cctggcgacc ggcgcagcgt tcgcctacac cgagcagctc aagctgacga
      721 gaagcccgat cctgggcacg agcgtcacca agctcttctc gcccgtctgc gagtgcccca
      781 ccgacgtggc cacgatcagc ttccgcctgc gcgacgaaga ccggctcacg gtcgagatga
      841 tcgaccgcac gggcaccccg gtccgcgagc tggttcgccg gcaagccgag ccggcagggg
      901 ctgtcgatct catctgggac ggtcgcgacg acgcaggcgt cgtcgtgccg gagggggctt
      961 accgtccgcg ggtacgcctc aacgacgatc gccgcactat cacactgccg aatccgatcc
     1021 gggtcgacgt gactccgccg cgtttcgagc gcttctccat ccgcccgcgc gtcttctctc
     1081 cggacgggga cgggcgcaac gaccgtgtgg tcgcgcgcta ccgggtcagc gagcgggctc
     1141 gggtgtcgct gttcgtggac ggagtgcgcc gcgtcctcaa gcgcggcagc aggcgcgacg
     1201 gcgcgatcag ctggttcggg cgaatcggcg gcgagtccgt ggcgcccggg ccgtacgtcc
     1261 tcactctcgg cgcgcgagat ctcgccggca acacctcccg gcggacggcg ccgcgcacgg
     1321 tcgccgcccg ctacgtcgcg ctcggtcgca aggtgatcga gacgcggccc aatgcacgct
     1381 tcgccgtgct ggtcgccagc gacgcgcggc gcatccgctg gcagctcggg cagcggacgg
     1441 ggacgggccg gcccggcacg ctcaggctgc gggcgccctc caggccgggg cggtacacgc
     1501 tcacggtgac cgcgagcggg aacagcgtcc gggccgccgt gttcgtacgg gatctggccg
     1561 gatgagcccg gcagagatct cggccctgat cggctgcggc gggcttgcgc tcctcgtcct
     1621 cgccccgcgc cgtgatctgc gtgtcgtcgg gctcgtcgcc tgggccgctg gtctcgcagg
     1681 cctcgtcgtc tacctcgctc cggacacttc gacgactcgc ctcgcggcag cggcggtcgg
     1741 cggcctcgtc ttggccggcg ccggcggctg gctgctcaac cgcttcccgt atctgctcgc
     1801 gttcgcgacg ctcgcgtgcc tcccggcgcg cctcccgatc aagctcggcg acgaggaggc
     1861 caacctgctc gtgcctctct acggcgtcgt ggccgcctac gccgtcgctc tcggctggca
     1921 gctggtcgtc cgcaaggacg atcgttcgcg ggagcttggg ccgatcgcgt ggccgctcgc
     1981 gctcttcgtc gcctgggcgg gggtggcgat gctctgggcg gacgatctgc gtgaagggag
     2041 catcttcgtc ggcgcgttcc tactcccctt cgcgctgttg gccgtcggct tctcgcggtt
     2101 gccctggcgg gggcggtggc tgacgtggct ctggggcgcg ctcgtagcca cggcgctcgc
     2161 gtacgcgacg gtcgggatgt tccagtgggc gacccgcgac gtcttctgga atcccaaggt
     2221 gatcgtcggc aacgcgtacc tcccgttctt ccgcgtcaac tcgatcttct gggatccgtc
     2281 gatctacggg cggtacctcg ccgtcgggat cctcaccgct cttgccgggc tgctcctcgg
     2341 cggcatccgc gggaagaagc tcctcggtct ctatgccgtc atcggcgccc tctgggtcgg
     2401 cctcttcttc acgttctctc agtcgagcta cgtcgctctg gcagtcggcg tcgtcgtcgc
     2461 ggcggtcgtc gtctggcgct ggcgggccgt cgtaggcgcc acgatcctcg tcgttctcgt
     2521 cgtggccggg acgctcgcgc tgccggacgt ccgtgacagg ctgatcgacg aggggcggtc
     2581 gggtgtcaac gccgtcacca gcggtcgcgc ctcgctcgtc ggacaagggc tgcgcatcgc
     2641 cgtcggtgat cccgtcgggg gcgtcggcac gggtgggttc aagcgtgcgt acgccgaccg
     2701 ggtcgggctt gtcggcgaag acccgaagcg agccgcctcg catacgacgc ccgtgacggt
     2761 cgcggccgag aacggcgtcg tcgggctcgc ctttttcgcc tggctgctcg cggccgccct
     2821 cttcgcgacg ctgcgggggc tcggctcggg cttcacctcg cgcgtgtcgt tcgccgtcgg
     2881 ccttacgctt ctcgcgatca cggtgcactc gctcttctac aacgcctttt tcggcgaccc
     2941 gatgacctgg gcgctcttcg gcctcgtcgg ccttgcggcg agcgtccccc gacgcgccac
     3001 cgccgaggag acctcgcgag ccgttcccgt gcccgagccg gagcccgtcc gggagccaca
     3061 acccgccgcg accggaggag caccgtgatc ggaggctcca agcgtgcact cgtgctcgcg
     3121 ccgcacaccg acgacggcga gttcggatgt ggcggcacga tggcccgcct cgtagacgaa
     3181 gggtgcgagg tacgctacgt cgccttctcc atcgcgactc gctccctgcc ggcaggtttt
     3241 ccgcccgaca cgctcgcgca agaggtgcgc ggggcaaccg ccgagctggg catccccgag
     3301 tcggcgctca ccgtgcacga cttcgacgtc cgcaccttcc cggaacaccg gcaggacata
     3361 ctcgagctct tggtcgagct ctgggaggaa tggcggcccg acgtcgtctt ccagccctcg
     3421 caccacgatg tccaccagga tcatcagacg atcgcgcagg agggcctccg ggccttcaag
     3481 cggacgacga tcctcgggta cgagatcccg tggaacaact acgacttcgc gtacggctgc
     3541 tacgtggcgc tcgccgagtc gcacatcgag cgaaaggtcg ccgccgtcca gcggtacgcg
     3601 tcgcaacagc accggcgcta cgccaacgcc gactacatct ggaacctggc ccgagtgcac
     3661 ggcaccaacg tgaaccgcga gtacgcagag gtctttcagg tgtaccggat cgttgcatga
     3721 cgcacatgta agcgttttct cacgcagaaa acgcaaacgt ccgctcggcg ggaatacttg
     3781 gagggatgca ccgactctcg gtgagcatgc gatggtggct ggcgctggtc ttcgccgcca
     3841 tggtcgccat gaccgcagtc gtcgtggccc aggtgctcgc c
//
LOCUS       DBNGNK010000336         5060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_59637,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000336 DBNGNK010000000
VERSION     DBNGNK010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_59637"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..300
                     /locus_tag="AC150V_09935"
     CDS             82..300
                     /locus_tag="AC150V_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3763277.1"
                     /translation="MTLTTLVSGVNGTSGADLLLGTAGADNMTANAGNDCVLGGGGDD
                     TINGGAGTDVCIGGPGTDTFINCETQIQ"
     gene            complement(359..2299)
                     /locus_tag="AC150V_09940"
     CDS             complement(359..2299)
                     /locus_tag="AC150V_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR3763278.1"
                     /translation="MATTTQDELRIAEIPTDVEIDPVTLDIIEGALKSARFEMDAVLF
                     RSAMSPVIREQHDEFPMLTDPRGRMVVGQFGAYINEMMEEWDRGIYPGDVILTSDPFK
                     CSASISHTNDWLVLVPIFYEDELVGWSSQFGHQMDCGGPLPGSLPTGARTIFEEGIII
                     PPLKIIERGVPQEDVLKLIFNNVRLPEMNRADLFAIVAGCRAGEKRVVELCDRFGKET
                     YLAALQALLDRTYEAMRKLILVAIPEVPQTFVDYVDDDGLGNGPFKMQLTIWREGDQA
                     FFDWSGTDPQALGPVNFYLSEGMFKMFIGVYLIMVNDPQILFNDGFYPLLHVVMPKGS
                     LLNPRFPAALGCRTHALARLFDVLGGALVKQAPELNTASGYGTSPYMLYSGWDEHGDF
                     FYSMEILYGGIPGRPIGDGMDGHSWWPLFENIPTEYLEAYYPMRIDGYTTVTDSGGPG
                     FHRGGNGVEKRYVYLEPGEVSIHDDRWLTRPWGVLGGLPGARSSKLLVRADGTEEALP
                     SKCDQVQVEPGDMLVYRTAGGGGWKDRLDRDPAAVARDVSFGLVSSENAKDGYGVILA
                     DDGSVDEAATEKERERQRTERGEALAFDFGPSLEEILATCKEETGLDPPVPAKPLRWS
                     PLEPGDEALARVREGDGIPEPS"
     gene            complement(2308..4368)
                     /locus_tag="AC150V_09945"
     CDS             complement(2308..4368)
                     /locus_tag="AC150V_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3763279.1"
                     /translation="MAYRLGVDVGGTFTDLFLVGDSNGGAQYRVKTPSTPADPSEGVL
                     TGVQRICGEAGIEVGELQNILHGTTVATNAVLEAKGARVGLITTNGFKQILHLARSQT
                     PGPLAGWIIMIKPDPPASLADTREAVERMDARGETIVPVDEEQVEAIVRDLVESGVES
                     LTVGLINSYVNPAHEEQIGAIVERLYPDFPVTLSAHVLPEFREYERTLTACMNSYVRP
                     NVANYISELQSKLKGVGASSDVNILRSDAGLMTTTEATRNPIYGILSGPSGGVAGALY
                     VARKAGFENILTFDMGGTSTDVALCQNGEPTIGRETSISHFRIKVPSVNVHTVGAGGG
                     SIAHVPELTKALRVGPESAGADPGPAAYGRGGENPTVTDANVVVGHLPPQLLGGEMQL
                     DVDAARAAVQKVAEAMGLDSAEEAAEGILAIVNENMAGALRLVSVQRGHDPRDFALVA
                     YGGAGPLHANAVAEIMGSFPVLIPPAPGLLCAIGDLVADFRDEFAQTYIRVLPEADPG
                     DVATILETLGERATEWLDGEGIGKDARGIEYTADMRYHRQGYEIPVAIDGDEVKAHGL
                     TGLEERFNLLHEQLYGFRMEETACEIVNLRAVGFGAVPKPELPTGELGADDAAGAVLE
                     EHAILFEGENVPTKIYDRAKLAPGMRFDGPAIVTEFDSTTVVLPGYHARVDENYNILI
                     DRGS"
     gene            complement(4383..>5060)
                     /locus_tag="AC150V_09950"
     CDS             complement(4383..>5060)
                     /locus_tag="AC150V_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR3763280.1"
                     /translation="YAGLVLNERGYDRLVTTGLDEVHFAFAVSETFNEKNAGSAVEDT
                     VAAIERMLARTREDGIRATVTLGTSFGCPFEGAIDPGRVLELADRFVAAQADEIVLAD
                     TVGVGVPRQVRDLVGRATALGPPIGIHLHNTRSTGFANAYAALEAGATVFDASVGGIG
                     GCPFAPRATGNIATEDLVYLLHGEGVETGIDLGQLIEVAEWLEGVLERELEGQVYRAG
                     TFAPVAG"
BASE COUNT          775 a         1744 c         1716 g          825 t
ORIGIN      
        1 aacaccgtcc cgggctccag gggctcgaga ttcacgacgg cgatcagcgc aaacaccctc
       61 aaaccgtccg cctgcgccgc gatcacactc acgacgctcg tctccggcgt caacggcaca
      121 agcggcgcgg acctcctgct cggcaccgca ggcgcggaca acatgaccgc gaacgccggc
      181 aacgattgcg tcctcggcgg cggcggcgac gacacgatca acggcggcgc aggaaccgac
      241 gtctgcatcg gcggccccgg caccgacacg ttcatcaact gcgaaacgca aatccagtag
      301 ctgcggcgca gcgttcggaa atgtgcggcg gggcgggccg acgcccgccc cgccgacctc
      361 aggacggctc ggggatgccg tcgccctccc ggacgcgtgc caacgcctcg tcgccgggct
      421 cgagcggcga ccagcgcagc ggcttcgcgg gcaccggggg atcgaggcca gtctcctcct
      481 tgcaggtcgc gaggatctcc tcgagcgaag gcccgaagtc gaaggccagc gcctcgccgc
      541 gctcggtgcg ctgccgttcg cgctccttct ccgtcgcggc ctcgtcgacc gagccgtcgt
      601 cggcgaggat cacgccgtag ccgtccttcg cgttctcgct gctgacgagt ccgaacgaga
      661 cgtcgcgcgc gaccgctgcc ggatcgcgat ccaaacggtc cttccagccg cccccgccgg
      721 ccgtccggta cacgagcatg tcgccgggct cgacctgcac ctggtcgcac ttcgacggca
      781 gcgcttcctc ggttccatcg gcacggacga gaagcttcga ggagcgggca ccgggcaggc
      841 cgccgaggac gccccagggt cgggtcagcc agcggtcgtc gtggatcgag acctcgcccg
      901 gctcgaggta gacgtaccgc ttctcgactc cgttgccgcc tcgatggaag ccggggccgc
      961 cggagtcggt caccgtcgtg tagccgtcga tccgcatcgg gtagtacgcc tcgaggtact
     1021 cggtcgggat gttctcgaag agcggccacc aggagtgccc gtccatcccg tcgccgatcg
     1081 gccggccggg gatcccgccg tagaggatct ccatcgagta gaagaaatcc ccgtgctcgt
     1141 cccagcccga gtagagcatg tacgggctcg tgccgtagcc ggacgccgtg ttcagctcgg
     1201 gcgcctgctt gacgagtgcg ccgccgagga cgtcgaagag ccgtgccagc gcatgcgttc
     1261 ggcagccgag cgccgccggg aagcgcgggt tgagcaggct ccccttcggc atcaccacgt
     1321 gcaggagcgg gtagaagccg tcgttgaaga ggatctgagg gtcgttgacc atgatcaggt
     1381 agaccccgat gaacatcttg aacatcccct ccgagaggta gaagttcacg gggcccaagg
     1441 cctgtggatc ggttccggac cagtcgaaga aggcctggtc gccttcacgc cagatggtga
     1501 gctgcatctt gaacgggccg ttgccgaggc cgtcgtcgtc gacgtagtcg acgaacgtct
     1561 gcggcacctc ggggatcgcg acgaggatca gcttccgcat cgcctcgtac gtgcggtcga
     1621 ggagcgcctg cagcgcggcg aggtaggtct ccttgccgaa gcggtcgcac agctcgacga
     1681 cacgcttctc gcccgcccgg cagccggcga cgatggcgaa gaggtcggcg cggttcatct
     1741 ccggcaggcg gacgttgttg aagatcagct tcagcacgtc ctcctgcggc acgccccgct
     1801 cgatgatctt gagcggcggg atgatgatgc cctcctcgaa gatcgtccga gccccggtgg
     1861 gcagcgagcc cgggagcggc ccgccgcagt ccatctggtg gccgaactgg ctcgaccagc
     1921 cgaccagttc gtcctcgtag aagatcggga ccagcaccag ccagtcgttg gtgtgggaga
     1981 tcgaggccga gcacttgaag gggtcggacg tgaggattac gtcgccggga tagatgcccc
     2041 ggtcccactc ctccatcatc tcgttgatat aggcgccgaa ctggccgacg accatgcggc
     2101 cgcgcgggtc ggtgagcatt gggaactcgt cgtgctgttc acggatgacc gggctcatcg
     2161 ccgagcggaa gagcacggcg tccatctcga agcgggcgct cttcagtgcc ccctcgatga
     2221 tgtccagtgt gacggggtcg atctcgacgt cggtcgggat ctcagcgatc cggagctcgt
     2281 cctgtgtggt ggttgccatg gcgtgcctca gctcccccgg tcgatgagga tgttgtagtt
     2341 ctcgtccacc cgcgcgtggt agccgggcag gacgacggtc gtggagtcga actcggtgac
     2401 gattgccggg ccgtcgaagc gcatgcccgg agcgagcttc gcccggtcgt agatcttcgt
     2461 gggcacgttc tcgccttcga agaggatcgc gtgctcctcg aggacagccc cggctgcgtc
     2521 gtcggcgccg agctcgcccg tcggcagctc gggcttcggc accgcgccga acccgacggc
     2581 gcggagattg acgatctcgc aggccgtctc ctccatgcgg aagccgtaca gctgctcgtg
     2641 gagcaggttg aagcgctcct cgagcccggt gagcccgtgc gccttcacct cgtccccgtc
     2701 gatcgcgaca gggatctcgt atccctgccg gtggtagcgc atgtcggccg tgtactcgat
     2761 gcctcgcgcg tccttgccga tgccctcgcc gtctagccat tccgtggcgc gctcgcccag
     2821 cgtctcgagg atcgtcgcga cgtccccggg atctgcttcc gggagaacgc ggatgtacgt
     2881 ctgcgcgaat tcgtcccgga agtccgcgac gaggtcgccg atggcgcaga gcaggcccgg
     2941 ggccggcggg atgagcaccg ggaacgagcc catgatctcc gcgacggcgt tcgcgtgcag
     3001 agggcccgcg ccgccgtagg cgacgagagc gaagtcgcgg ggatcgtgtc cacgctggac
     3061 ggagacgaga cgcaacgcac cggccatgtt ctcgttcacg atcgcgagga tcccctcggc
     3121 agcctcctcc gccgagtcga ggcccatcgc ctcggccacc ttctgtaccg ccgcccgcgc
     3181 ggcgtcgacg tcgagctgca tctcgccgcc cagaagctgt ggcgggaggt ggcccacaac
     3241 gacgttcgcg tccgtgaccg tggggttctc gccgccgcgg ccgtaggcgg ccggccccgg
     3301 atcggcacct gccgactccg gccccacgcg gagcgccttg gtcagctctg ggacgtgggc
     3361 gatcgagccg ccgccggcgc cgactgtgtg cacgttcacc gacggcacct tgatgcggaa
     3421 gtggctgatc gacgtctcgc gcccgatcgt cggctcgccg ttctggcaga gcgcgacgtc
     3481 ggtcgaggtg ccgcccatgt cgaaggtgag gatgttctcg aatcccgcct tgcgcgccac
     3541 gtagagggct ccggcgacac cgcccgaagg gccggagagg atgccgtaga tcgggttgcg
     3601 cgtcgcctcc gtcgtggtca tcaggcccgc gtcggagcgg aggatgttga cgtcagagct
     3661 tgcgcccacc cctttcagct tcgactgcag ctcgctgatg tagttggcca cgttgggtcg
     3721 aacgtacgag ttcatacacg cggtcaacgt gcgctcgtac tcgcggaact ccggcagcac
     3781 gtgggcggac agggtcaccg ggaaatcggg gtagagacgc tctacgatcg ccccgatctg
     3841 ctcctcgtgc gcggggttga cgtaggagtt gatgaggccc acggtcagcg actcgacgcc
     3901 ggactcgacc aggtcgcgaa cgatcgcctc gacctgctcc tcgtcgacgg gcacgatcgt
     3961 ctcgccgcgc gcgtccatcc gctcgaccgc ctcgcgcgtg tccgcgaggg acgccggcgg
     4021 gtcgggcttg atcatgatga tccagccggc aaggggcccg ggtgtctgcg agcgcgccag
     4081 gtgcaggatc tgcttgaagc cgttcgtcgt gatgaggccc acgcgggcgc ccttcgcctc
     4141 gagcaccgcg ttcgtcgcca ctgtcgtccc gtgcaggatg ttctgcagct cgccgacctc
     4201 gattccggcc tcgccgcaga tccgctgaac cccggtgagc acgccctccg aggggtctgc
     4261 gggcgtcgac ggggtcttga cgcggtactg ggcgccgccg ttcgagtctc cgacgaggaa
     4321 caggtcggtg aacgttccac cgacgtcgac cccgagcctg tatgccatct tcttcctccg
     4381 tttcagcccg cgacgggagc aaaggtcccg gcacgataga cctgtccctc gagctctcgc
     4441 tcgagtacgc cttcgagcca ctcggcaacc tcgatcagct gcccgaggtc gatacccgtc
     4501 tcgacgccct cgccgtggag cagatagacg agatcctccg ttgcgatgtt gcccgtcgcg
     4561 cgcggcgcga aggggcaacc gccgatgccg ccgaccgagg cgtcgaacac cgtcgcgccg
     4621 gcctccagag ccgcgtaggc attggcgaaa ccggtgctcc gcgtgttgtg caggtggatc
     4681 ccgatcggcg gcccgagcgc ggtggccctg cccacgagat cgcgtacctg ccgcggtacg
     4741 ccgacgccca ccgtgtcggc gagcacgatc tcgtcggcct gcgcggcgac gaagcgatct
     4801 gccagctcga gcacgcgccc ggggtcgatc gcaccctcga acggacagcc gaacgacgta
     4861 ccgagcgtca cggtggcgcg gatgccgtcc tcgcgcgtgc gcgcgagcat gcgctcgatc
     4921 gccgcgacgg tgtcctcgac cgcgctgccc gcattcttct cgttgaacgt ctcggagacg
     4981 gcgaaggcga aatgcacctc atcgaggccg gtcgtcacga gccggtcgta gccgcgctcg
     5041 ttgagcacga gccccgcgta
//
LOCUS       DBNGNK010000337         7868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_202688,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000337 DBNGNK010000000
VERSION     DBNGNK010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_202688"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="AC150V_09955"
     CDS             complement(<1..141)
                     /locus_tag="AC150V_09955"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR3763281.1"
                     /translation="MTLVTLFVAEGCQLCREARAVLEELRSELAYELRVVDIDGDEEL
                     ERA"
     gene            complement(141..1436)
                     /locus_tag="AC150V_09960"
     CDS             complement(141..1436)
                     /locus_tag="AC150V_09960"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR3763282.1"
                     /translation="MSDLPGAARSGETAPSHLRENPFELARRQLYLVAETFGVDDDVV
                     RMLEQCKRCLEVSIPVVMDSGEVRVFEGHRVTHNDTRGPAKGGIRYHQDVTRDEVKAL
                     AMWMTWKCALTGVPFGGAKGGVVCNPKELSRGELERMTRRYTTEIINEIGPELDIPAP
                     DVGTDAQVMAWIFDTYSMNVGHSVLEVVTGKPLSIGGSQGRHDATARGCMNAIQALHA
                     KQGRSLAGLTFAIQGFGNVGANLARLLAEEGAMIVALSDSAGGVHASTGLDVTAAIAH
                     KEEAGELTGLANAEAVTNEELLELECDVLAPCALEQVITEENAGRVRAAVVAEGANGP
                     VTPAADEILEDRDIFVLPDVLANAGGVVVSYFEWVQGIQEWTWKEAEVDERLREIVTE
                     AFEEAWQTRDRHRTSMRMAAYGLGVQRVAEATTTRGIYP"
     gene            complement(1456..3999)
                     /locus_tag="AC150V_09965"
     CDS             complement(1456..3999)
                     /locus_tag="AC150V_09965"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3763283.1"
                     /translation="MPMPRRPDQLLFPVLALALAAAAVLLAGGGSGSSGPTPAPSAAG
                     WRTLLGDRPAPLLGQRQIVVLKAASLADRVARVGGVASEEQMRAWTRTAREKQQVVLN
                     RLAFKGAPISPEQSYVRVLNGFAASLDARSASLVERDAGVAGVYPVRAAYPAAADEEE
                     GLAAGRRAGIELPGFDGAGITIALLDTGVDRTHPYLRERLLDGIDVVDPGGDATARLH
                     PTVVGRQEQHGTELAGLIVGALGPVDLAGVAPGASLLPIRVAGWQPDATGGVAVYSRT
                     DQLLAGIEAAVDPDESGDSHDAARVAVVGVAEPFAAFAEGPLAQAASGAATLDTLVVA
                     PVGNDGLAGPSFGSASGPGGAPAALTVAAVDTRRESRSVHVFLRAGLGILLSGEQPLG
                     GAFGPTKSDTLPVVVVQKGRVEASEGPRGASAFFGDRGYSVVAGTAPLLPRGATSPEA
                     VRAAAVAGAGAILVDGPLPAGALSLDESVQVPILGIPSAVAKQIRAQRRAGTQVVVSI
                     GAPKLKVNPGAGAAAAFSSRGLGFDGGLKPELGAPGVGLATSAPGRMPGQGPRYGVVS
                     GSSAAAAVAGAAAALLAQARPDLDAVALKSALIGSAVRLRGETGATGAGMVDVEAAAA
                     TEVVAVPGAIGLPPETPSTEESSMPLTLRSVSSRPLDVVLSVDRNAAGTLLRVTPGKI
                     RLKPGKAVDVRVTVLPGTRPRPPSAMRGTIRIKPDDAPWTRVPWALALPVEGRPVLRD
                     VKLSRKVFKSDGSQAVILSLVAGRVDGTVAKPQVLALSQLDIQLLRDGKRLGRLARVR
                     NVLPGRYAFGLTGRDPAGNKLAEGIYELRVVGYPVSGAVGEARVSFTIR"
     gene            complement(3977..4942)
                     /gene="tsaD"
                     /locus_tag="AC150V_09970"
     CDS             complement(3977..4942)
                     /gene="tsaD"
                     /locus_tag="AC150V_09970"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR3763284.1"
                     /translation="MILGIETSCDETAAALVSTDGEVLSSVVASQAELHSRYGGVVPE
                     VASRRHLELVSPVVGETLNEAGVELGDVEKIAVTRGPGLVGALLVGLSAAKAIAWARR
                     LPLVPVDHLQGHVASLYLEPDPLEPPFVCLLATGGHTMVLAVRRRGHVELLGTTLDDA
                     AGEAFDKGARMLGLGYPGGPAIDRVAREGDAAAFSFPVARVEGLDFSFSGLKTALLYA
                     VRDLQPDELERCRADLAASYQAAILRALTTRLREAAATARLSRVAVVGGVAANSQLRA
                     ALPDAAFAPLPYCTDNAAMIASAARFVDPIPYPRYLGLDAYASAA"
     gene            complement(4939..5433)
                     /gene="rimI"
                     /locus_tag="AC150V_09975"
     CDS             complement(4939..5433)
                     /gene="rimI"
                     /locus_tag="AC150V_09975"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR3763285.1"
                     /translation="MNLEIRNLQLEDLDAIEMIERASYPAPWSRSMFASELAKPSSIS
                     VGACRRGELVGFLIISRYVDAWHVMNLAVAPPHRRLGVASTLLERLFDLTRDDPRRGY
                     TLEVRVSNIEAIQLYERHGFRSRGVRRGYYTDNREDALIMWRDPAEPAVPGATPAGGS
                     AESA"
     gene            complement(5430..6017)
                     /gene="tsaB"
                     /locus_tag="AC150V_09980"
     CDS             complement(5430..6017)
                     /gene="tsaB"
                     /locus_tag="AC150V_09980"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR3763286.1"
                     /translation="MLILAFDTATSVSTCALVADGEVLGERTSVPAALLEDVDAVLRR
                     GGAGAADIDGVAVGTGPGSFTSTRIGLAYARGLGIALRIPVAGVSTLDALAAGADGAF
                     PVVDARRGEVFIPGPLAVDPASLALEAGILCVGDGARRYRDVLEAMGANVPADDAAVH
                     LPLARHHAALAEHFGPAEAVEPIYVRAPDVDEAIA"
     gene            complement(6057..6506)
                     /gene="tsaE"
                     /locus_tag="AC150V_09985"
     CDS             complement(6057..6506)
                     /gene="tsaE"
                     /locus_tag="AC150V_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR3763287.1"
                     /translation="MRVEVVTTSAAATESLAGRLAARLRPGDVVTLSGELGSGKTTFV
                     RGACRALGVQGPVTSPTYTIGHRYTGRFDVSHLDLFRFADVSPAEWGDIEPYFDDALA
                     LVEWPEAGLGVLPAARVAVTLEHVDPTTRRIRLDAAEAALLQDLDAP"
     gene            complement(6510..7187)
                     /locus_tag="AC150V_09990"
     CDS             complement(6510..7187)
                     /locus_tag="AC150V_09990"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010936214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR3763288.1"
                     /translation="MSDAVDRARALNGYAAETSGCACCALSQGRTNVVFGDGNPDADL
                     MFVGEAPGFHEDRQGVPFVGQAGKLLDGLLERVGMTRADIYIANVLKCRPPGNRDPLP
                     EEIGSCEPHLFRQIELIQPRVVATLGNFATQLLSGRPHGITRVHGSEQHVTVAGATVL
                     LYPLYHPAAALYTPSMLSVLEEDFSRIPDLIRGAGRSDALPPPDDDRDRVLVAAGEPE
                     AVQLGLF"
     gene            complement(7224..>7868)
                     /locus_tag="AC150V_09995"
     CDS             complement(7224..>7868)
                     /locus_tag="AC150V_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR3763289.1"
                     /translation="SGAKVTTSTPSPGRLATEYERLRADGAAHVLSVHVDSRVSAVVD
                     AARLGAAECTAEVTVVDTGQTSFGVALCALEARRVLEAGGGSDEAVARSRWRAAAIEN
                     VFVVGEGVGGRIGVGGGTVLSFARGSPDPLKSASDEGEAVAAMTEHVASRSGSLGVAV
                     GHAHPALEASADVLADALGSLPTVGEVCRYRVGPSVAAHTGAWSFGAFFWPLDD"
BASE COUNT         1228 a         2812 c         2712 g         1116 t
ORIGIN      
        1 ggcgcgctcg agctcctcgt ctccgtcgat gtcgacgacg cggagctcgt aggcgagctc
       61 ggaacggagc tcctcgagca cggcgcgcgc ctcgcggcag agctgacagc cctcggcgac
      121 gaacaacgtc acgagcgtca tcagggatag atgccacgtg tggtcgtcgc ttctgcgact
      181 cgctgcacgc ccagcccgta ggccgccatg cgcatgctcg tgcggtgccg gtcacgcgtc
      241 tgccaggcct cttcaaatgc ctccgtcacg atctcacgca gacgctcgtc gacctcggct
      301 tccttccagg tccactcctg gatgccctgc acccattcga agtaggagac gacgaccccg
      361 ccggcattgg cgagcacgtc cggaaggacg aagatgtcgc gatcctcgag gatctcgtcg
      421 gcggcgggag tgaccgggcc gtttgccccc tccgcgacga ccgcggcccg gacgcgccca
      481 gcattctcct cggtgatcac ctgctcgagc gcacacggcg cgagcacatc gcactcgagc
      541 tccagcagct cttcgttcgt cacggcctcc gcgttcgcga gcccggtgag ttcacccgct
      601 tcttctttgt gcgcgatcgc cgccgtgacg tcgagcccgg tggaagcgtg gactccaccc
      661 gccgagtccg aaagcgccac gatcatcgcc ccctcctctg cgagcaggcg ggcgaggttg
      721 gcgcccacgt tgccgaagcc ttggatcgcg aaggtcagtc ctgcgagcga tcgcccctgc
      781 ttcgcgtgga gcgcctggat tgcgttcatg cacccgcgcg cggtcgcgtc gtggcggccc
      841 tgcgaccctc cgatcgagag aggcttgccc gtgacgacct cgagaaccga gtggccgacg
      901 ttcatcgaat aggtgtcgaa gatccacgcc atcacctgcg cgtcggtgcc gacgtccggg
      961 gcggggatgt cgagctcggg gccgatttcg ttgatgatct cggtcgtgta gcgacgggtc
     1021 atccgctcga gctctcctcg tgacagctct ttcgggttgc acacgacgcc gcccttggcg
     1081 ccgccgaacg ggacgccggt gagcgcgcat ttccacgtca tccacatggc cagcgccttg
     1141 acctcgtctc gtgtcacgtc ctggtggtag cgaatgccgc ctttggccgg cccgcgggtg
     1201 tcgttgtgcg ttacccgatg cccttcgaag acgcggactt cgccactgtc catgacgacg
     1261 gggatggaga cctcgaggca gcgcttgcac tgctcgagca tccgaacgac gtcgtcgtcc
     1321 acaccgaagg tctcggcgac cagatagagc tggcggcgcg cgagctcgaa cggattttcg
     1381 cggagatgcg acggggctgt ctcacccgat cgggcagctc ccggaaggtc gctcacgcgc
     1441 gccgagtata ggacgtcagc ggatcgtgaa agaaacccgc gcctcgccca cggcccccga
     1501 caccggatat ccgaccacgc gcagctcgta gatcccctcg gcgagcttgt tgccagccgg
     1561 atcgcgtcct gtcagcccga atgcgtaccg acccggtagg acgtttcgaa cccgtgcgag
     1621 tctgcccaga cgcttcccgt ctcgcagcag ctggatgtcg agctgcgaca gcgcgagcac
     1681 ttgcggcttc gcgaccgtgc cgtcgacgcg tcccgcgacc agcgagagaa tcaccgcttg
     1741 cgatccgtca gacttgaaga ctttgcgcga cagcttcacg tctcggagca ccggcctccc
     1801 ttcgaccggg agggcgagcg cccagggaac gcgcgtccac ggtgcgtcgt cgggcttgat
     1861 cctgatcgtg ccgcgcatcg cactcggcgg cctggggcgt gttcccggga ggacggtgac
     1921 ccgcacgtcg accgccttgc ccggcttcag cctgatcttt ccgggggtga ctcggaggag
     1981 cgtgccggcg gcgttccggt cgaccgagag gacgacatcg agaggcctcg acgacacgct
     2041 gcgcagcgtg agcggcatgc tcgactcctc cgtcgagggg gtttcaggag gaagcccgat
     2101 cgcgccgggc accgcgacga cctcggtggc ggcggcggct tcgacatcga ccattcccgc
     2161 tcccgtcgcg cccgtctctc cacgcagccg aacggccgat ccgatcagtg cgctcttcaa
     2221 ggcgacggca tcgagatcgg ggcgcgcctg cgcgaggagc gcggccgccg cgcccgcgac
     2281 tgcggcggcg gcgctcgagc ctgagacgac gccgtaacgt ggcccctgcc ccggcatccg
     2341 gcctggggcc gacgtcgcca gcccgacccc aggtgccccg agctcgggct tgagcccccc
     2401 gtcgaagccc agcccacgcg agctgaacgc ggctgccgca cccgcgccgg gattgacctt
     2461 cagcttcggc gcgccgatcg acacgacgac ctgggtgccc gcgcggcgct gagcgcggat
     2521 ctgttttgcc acggcggacg ggattcccag gatcggcacc tggaccgact cgtcgaggct
     2581 cagcgcgccg gcgggaagcg gcccgtcgac gagaatcgct cccgcacccg ccacggccgc
     2641 cgcccgtacc gcctcgggcg aggtcgcacc acgcggaagg agtggtgccg taccggccac
     2701 gacgctgtag ccccgatcgc cgaagaaggc cgacgcgccg cgcggcccct cggaggcttc
     2761 cacacggccc ttctgcacaa cgacaaccgg gagcgtgtcc gacttggtcg ggccgaaagc
     2821 gcctccgagc ggctgctcac cggacagtag aatgcccagt cccgcccgca ggaagacgtg
     2881 caccgaccgg ctctcccgcc gcgtgtcgac ggcagctacg gtcaatgcgg cgggagcgcc
     2941 ccccgggccg ctggcgcttc cgaagcttgg tcctgcgagc ccgtcgtttc cgaccggagc
     3001 gacgacgagg gtgtcgagcg tcgcagctcc ggacgctgcc tgggcaagcg ggccctcggc
     3061 gaaggcagcg aacggctcgg cgactccgac cacggcgact cgggcggcat cgtgggagtc
     3121 accgctctcg tccgggtcga ccgctgcctc gatccccgcc agaagctgat cagtacgcga
     3181 atagacggcg acgccacccg tcgcgtcggg ctgccagccg gcaacgcgaa tcgggagcag
     3241 cgacgcgccg ggcgccacac cggctaggtc gactgggccc agcgcgccga cgatgagacc
     3301 agccagctcc gtgccgtgct gctcctgcct gcccaccacc gtgggatgca gacgggccgt
     3361 cgcgtcgccg cctggatcga ccacgtcgat gccgtcgagc agccgctcgc ggagatacgg
     3421 atgcgtcctg tcgactccgg tgtccagcag cgcgatggtg atgcccgcgc cgtcgaaccc
     3481 aggcagctcg ataccggctc ggcgtccggc cgccaggcct tcctcctcgt cggcggccgc
     3541 cgggtacgcg gcgcgaaccg ggtagacgcc ggcgacgccg gcgtcccgct cgacgaggga
     3601 ggccgaccgt gcgtcgagcg atgcggcgaa cccgttgagc actcggacgt acgactgctc
     3661 cgggctgatc ggggctccct tgaatgcgag acggttgagt acgacctgct gtttttccct
     3721 cgccgtgcgt gtccaggccc gcatctgctc ctcggaggcg actcctccga cgcgcgcaac
     3781 gcggtcggcc agcgaagcgg ccttgagcac gacgatctgc ctctgcccga gcagcggtgc
     3841 gggccggtct cccagcaagg ttctccagcc ggcggcgctc ggagccgggg tgggcccgct
     3901 cgagccggat ccgccaccgg cgagcagcac cgccgcggct gcgagcgcga gggcaagcac
     3961 cgggaagaga agctgatcag gccgccgagg cataggcatc gagccccaga taccggggat
     4021 acgggatcgg gtcgacgaac cgggcggccg aggcgatcat cgccgcgttg tcggtgcagt
     4081 agggaagcgg cgcgaacgcc gcgtcgggta gtgcggcacg aagctgcgag ttggcggcga
     4141 cccctccgac taccgcaacc cgactcagcc tcgccgtggc ggccgcctcc cgaaggcgcg
     4201 tcgtgagcgc ccgcaggatc gcggcctggt acgaggcagc gaggtcggcg cggcaccgtt
     4261 cgagctcgtc cggttgcaga tcgcgcaccg cgtacagcaa ggcggtcttg aggcccgaga
     4321 acgagaagtc gagaccctcg accctcgcga ccgggaagga gaaggcagcg gcgtcgccct
     4381 ccctggcgac gcgatcgatc gcaggacccc ccgggtatcc gagacccagc atgcgcgcgc
     4441 ccttgtcgaa cgcttcaccg gccgcatcgt cgagcgtcgt gccgagcagc tcgacgtgtc
     4501 cgcgcctccg gacggcgagc accatggtgt ggccgccggt cgccaggagg cacacgaaag
     4561 ggggctcgag cgggtcgggc tccaggtaga gggacgcgac gtggccctgc aggtggtcga
     4621 cagggacgag cgggagtcgg cgtgcccacg cgatcgcctt ggccgcagaa aggccgacga
     4681 ggagcgcgcc gacgaggccc gggccgcggg tgacggcgat cttctcgacg tcgcccagct
     4741 ctacgcccgc ttcgttgagc gtttcgccca cgacgggaga gacaagctcg agatggcggc
     4801 gcgacgcaac ctcggggacg acgcccccat agcgggaatg gagctctgcc tgggaggcga
     4861 ccaccgacga gaggacctcg ccgtccgtgc tgaccagtgc cgccgcagtc tcgtcacacg
     4921 acgtctcgat tcccaggatc acgcgctctc cgcgcttcct ccggcgggcg tggcgcccgg
     4981 caccgcaggc tcggccggat cacgccacat gatcagcgca tcctcccgat tgtccgtgta
     5041 gtagccgcgg cgcaccccgc gggagcggaa gccgtgccgc tcgtagagct gaatcgcctc
     5101 gatgttcgac acgcgcacct ccagcgtgta cccgcgacgt gggtcgtccc tcgtcaggtc
     5161 gaagagccgc tcgagcaggg tggaggcgac ccccaggcga cggtggggcg gcgccaccgc
     5221 taggttcatg acgtgccagg catcgacgta tcgcgagatg atgaggaagc cgacgagctc
     5281 gccccggcgg cacgcaccga ctgagatcga gctcggcttg gccaactcgc tcgcgaacat
     5341 cgatcgcgac cagggcgccg gataggaggc acgctcgatc atctcgatcg cgtcgagatc
     5401 ctcgagctgc aggttccgaa tctcgaggtt cacgcgatcg cctcgtcgac gtcgggagcc
     5461 cgaacgtaga tcggctcgac agcctcggct gggccgaagt gctccgccaa cgcggcatgg
     5521 tgccgcgcca gcggcaggtg gacggctgcg tcgtcggcgg gaacgttcgc gcccatcgcc
     5581 tcgagcacgt ctcggtagcg acgggcgccg tcaccgacgc agagaatccc cgcctcgagc
     5641 gcgagcgacg caggatcgac ggcgagcggg cctgggatga acacctcgcc ccgccgcgcg
     5701 tcgacgaccg gaaaggcgcc gtccgctccg gcggccagcg catcgagcgt cgagaccccc
     5761 gcgacgggga tccgtagagc gatgccgagc ccgcgggcgt aggcgagacc gatccgggtc
     5821 gacgtgaagc tgcccggacc tgtgccgacg gcgacgccgt cgatgtccgc cgctcccgct
     5881 cctccccggc ggagcaccgc gtcgacgtcc tcgagaaggg cggccggaac cgaggtgcgc
     5941 tcaccgagaa cctctccgtc ggcgacgagg gcacaggtcg acaccgaggt cgcggtatcg
     6001 aacgccagga tcaacacggg cgacgcgctc gcgagccggc gggcgatgac ccgctctcaa
     6061 ggtgcatcca gatcttgtag caatgctgcc tcggcggcgt cgagccgtat ccggcgggtg
     6121 gtcggatcga cgtgctcgag cgtcactgcg acgcgagccg ccgggaggac gccgaggccg
     6181 gcctccggcc actcgaccag cgcgagcgcg tcgtcgaagt acggctcgat atctccccat
     6241 tccgccggcg agacgtccgc gaaacggaag aggtcgaggt ggctgacatc gaatcgtccc
     6301 gtataccgat gaccgatcgt gtacgtcggg ctcgtgacgg gcccttggac gccgagcgcg
     6361 cgacacgcgc cgcgcacgaa cgtcgtcttg ccgctcccga gctcgcccga gagcgtgacg
     6421 acgtcgccgg ggcgcagacg agcggcgagc cggccggcca gcgactcggt cgccgcagcc
     6481 gaggtggtga ccacctcgac cctcacgcct cagaagaggc cgagctgtac cgcctcgggc
     6541 tcgccggcgg caacgagcac ccggtcccga tcgtcgtccg ggggcgggag cgcgtccgaa
     6601 cggcccgcgc cgcggatcag gtcggggatc cggctgaagt cctcctcgag cacggagagc
     6661 atcgacggcg tgtagagggc ggcggctggg tgatagaggg ggtagaggag cacggtggcg
     6721 cctgcgacgg tgacgtgctg ctcgcttccg tgaacgcgtg tgatgccgtg cggccgcccg
     6781 ctgaggagct gggtcgcgaa gttcccgagg gtcgcgacga cccgcggctg gatcagctcg
     6841 atctggcgga acaggtgagg ctcgcacgag ccgatctcct cggggagtgg gtcgcgattc
     6901 cccggcggcc tgcatttcaa gacgttcgcg atgtaaatgt ccgcgcgggt catcccgacg
     6961 cgctcgagca gcccgtcgag gagcttcccg gcctgaccga cgaaaggaac accttgccgg
     7021 tcttcgtgaa agcccggcgc ctccccgacg aacatgaggt ccgcgtcggg attgccgtct
     7081 ccgaagacga cgttggtgcg accctgagaa agcgcgcaac atgcgcagcc ggaggtctcc
     7141 gccgcgtagc cgttcagcgc ccgcgctcga tcgacggcgt cgctcatgcg tgggcagtct
     7201 aggcagatat ccggacggct cgatcagtcg tccaacggcc agaagaacgc gccgaacgac
     7261 caggctcccg tgtgggcggc gacggaaggc ccgacccggt agcggcacac ctcgcccacc
     7321 gtcggcaggc tcccgagcgc gtcggcgaga acgtctgcgg atgcctcgag cgcggggtgc
     7381 gcgtggccga ccgcgactcc gagggagccg cttctgctcg ccacgtgctc cgtcattgcg
     7441 gcgaccgcct cgccctcgtc gctcgccgat ttgagcggat cgggagaccc tcgcgcgaag
     7501 ctgagcaccg ttcccccacc gacaccgatc cgaccgccga ccccctcccc gacgacgaag
     7561 acgttctcga tcgcggcggc ccgccatcga gaccgcgcga cggcctcgtc cgagccgcca
     7621 ccggcctcga gcacccgccg agcctcgagt gcgcaaagcg cgacgccgaa gctcgtctgc
     7681 cccgtgtcga cgaccgtgac ctcggcggtg cattcggcag cccccagtcg cgccgcgtcg
     7741 accaccgcag agaccctcga gtcgacgtgc accgacagca cgtgggccgc gccgtccgcg
     7801 cgtagacgct cgtactccgt cgcgagccgc cccggcgagg gcgtcgatgt cgtgactttg
     7861 gcgccgct
//
LOCUS       DBNGNK010000338         2639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1993647,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000338 DBNGNK010000000
VERSION     DBNGNK010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1993647"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="AC150V_10000"
     CDS             complement(<1..604)
                     /locus_tag="AC150V_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR3763290.1"
                     /translation="MRGLLQVLGDPQLAYPAVHVVGTNGKSTATRTIEALLLAEGLSV
                     GATVSPHVERWSERITVDGKEVDMEAVVGRVRDAAVALEATQFETITGAALAEFAHRR
                     VDAAVVEAGLGGRLDATNVLQTRVVLLTNVSREHTDVLGETLEQIAEEKLAVAHAGSL
                     VVLPDNTFATLVQARRVVIGGAREAAEAFLNRPIEAGVDVA"
     gene            complement(690..>2639)
                     /locus_tag="AC150V_10005"
     CDS             complement(690..>2639)
                     /locus_tag="AC150V_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012361.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR3763291.1"
                     /translation="LWGRGWIYRDNRIVNWCPYHHTAISDLEVVYEDMDDALTSLRYP
                     FADGDGTAGISVATVRPATILADVAVAVHPGDDRYAGAVGREVVVPYVEGRVPVIADE
                     AVDPEFGTGAVKVTPGHDPNDFEIGRRHGLPQPMVIGFDGRMTEDAGDLAGLTQEEAS
                     ARVLEWAKERRLVERRESYRHAVATCERCHTRIEPLISLQWWCAMAEAAKPAIAALRD
                     RRVRYHPESQHRFAIQSLEAAPEWCLSRQLWWGHQLPIWYCPDGHVTVQVDVPTACSE
                     CGSGELERDPDVLDTWFSSALWPYATLGWPDRTPELERYYPGDVNSTAREIIRLWENR
                     MIWTGLEVMGGVPFTDVIIHSTILAPDGRRMSKSLGTGIDPMDPIEQYGADATRYGLL
                     KISSTQDVRFSYGAIEEGRKLANKLWNVSRLIIAKAGSRSPALQPRELEERWILARLD
                     ATRREVEECWGRYDFATAMSALYHLTFDDFCDWYAEAIKPRLYDEEDDAVATALAALE
                     RLLALLHPVLPHVTEEIWSNLPDRTGRLIVAPWPEPNGRFAADIHALDRVQEAAQTFR
                     RSGIQTELATDDERRIFAAVVRPERARGDGDVEAERERLRKEVSRAEGMLANEKFVAN
                     APPEVVEAEREKLARYRRELEALGA"
BASE COUNT          398 a          934 c          886 g          421 t
ORIGIN      
        1 gggcgacgtc caccccggcc tcgatgggac gattcaggaa ggcctcggcg gcctctcgcg
       61 cgccgccgat gacgactcga cgggcttgga cgagcgtggc aaatgtgttg tccggtaaga
      121 caactaggct gcctgcatgc gcgacggcaa gcttctcctc cgcgatctgc tcgagcgtct
      181 cgcccagtac gtccgtgtgc tcgcgcgaga cgttcgtcag cagcacgacg cgggtctgca
      241 gcacgttcgt ggcgtcgagg cgtccaccca ggccggcctc gacgaccgcg gcgtcgactc
      301 gtctgtgggc gaactcagcg agcgcggcgc cggtgatggt ctcgaactgg gtcgcctcga
      361 gcgcgacggc cgcgtctcgc acgcgcccca cgacggcctc catgtcgact tcctttccgt
      421 cgaccgtgat tcgctcggac cagcgctcga cgtgcggcga cactgtcgcc ccgaccgaga
      481 gcccctcagc cagcaggagg gcctcgatcg tcctggtcgc cgtcgacttg ccgttcgtgc
      541 cgacgacgtg aacggcggga taggcgagct gaggatcgcc gagcacctgc aggagacctc
      601 gcatccgctc aaggccgaag ccgtctttcg gccaggggct cagaccggcg agccaggcga
      661 cagcagtctg cgccagcgct cctcctttcc tacgcgccga gagcttcgag ctcgcgccgg
      721 tagcgcgcga gcttctcccg ctcggcctcg acgacctcgg gcggcgcgtt cgccacgaac
      781 ttctcgttcg ccagcatgcc ttctgcgcgg gagacctcct tgcgcagccg ctcgcgctcc
      841 gcctcgacgt ccccgtcgcc gcgagcgcgt tccggccgga cgacggcggc gaagatccgc
      901 cgctcgtcat cggtcgcgag ctctgtctgg atgccgcttc ggcgaaacgt ctgggccgcc
      961 tcttggacgc ggtcgagcgc atggatgtcc gcagcgaacc ggccgttcgg ctcgggccac
     1021 ggcgccacga tgagcctgcc tgtgcggtcc ggcagattcg accagatctc ctccgtgacg
     1081 tgcggcagga cgggatgcag cagtgcgagc aggcgctcga gcgccgcgag cgcagtggcc
     1141 accgcgtcgt cctcctcgtc gtagaggcgc ggcttgatcg cctccgcata ccaatcgcag
     1201 aagtcgtcga aagtgaggtg gtacagggca ctcatcgcgg tcgcgaagtc gtagcgcccc
     1261 caacattcct cgacctcccg acgggtcgca tcgagacgag cgaggatcca gcgctcctcg
     1321 agctcacgcg gctggagcgc ggggctgcgg ctgccggcct tagcgatgat cagccgcgag
     1381 acgttccaga gcttgttcgc gagcttgcgg ccctcctcga tcgcgccgta cgagaagcgc
     1441 acgtcctgcg tcgaggagat cttcaacagg ccgtaccggg tcgcgtccgc cccgtactgc
     1501 tcgatcgggt ccatggggtc gatcccggtg ccgaggctct tcgacatccg tcgtccgtcc
     1561 ggcgcgagga tcgtggagtg aatgatcaca tctgtaaacg gaacgccgcc catgacctcc
     1621 aggccggtcc agatcatccg gttctcccac aggcggatga tctcgcgcgc cgtcgagttg
     1681 acgtcgcccg ggtagtaccg ctcgagctcc ggcgtgcggt cgggccagcc gagcgtcgcg
     1741 tagggccaga gggcggagga gaaccaggta tcgagcacgt ccgggtcgcg ctcgagctcg
     1801 cccgagccgc actcgctgca cgcggtcgga acgtcgacct gcaccgtgac gtgaccgtcg
     1861 gggcaatacc agatcggcag ctggtgcccc caccagagct gccgcgacag acaccattcg
     1921 ggggcagctt ccagggactg gatcgcgaag cgatgctgtg actccgggtg gtagcgaacg
     1981 cgtcgatcgc gcagcgccgc gatggccggt ttcgcggcct ccgccatggc gcaccaccac
     2041 tgcagcgaga tgagcggctc gatccgcgta tgacagcgct cgcacgtggc gacggcgtgc
     2101 cgatagctct cgcggcgctc gacgagccgg cgctcctttg cccactcgag cacacgcgcg
     2161 gaggcttcct cctgcgtgag gccagccagg tctcccgcgt cctcggtcat ccggccgtcg
     2221 aacccgatca ccatcggctg cggtagcccg tggcgccggc cgatctcgaa gtcgttcggg
     2281 tcgtggccgg gcgtgacctt gacggcgcca gtgccgaact cggggtcgac cgcctcatcg
     2341 gcgatcacgg gcacacggcc ctcgacgtag gggacgacga cctcacgccc aaccgcgccc
     2401 gcgtagcggt cgtcgcccgg gtgcacggcg acggcgacgt cggcaaggat cgtcgccggc
     2461 cgaaccgtcg caaccgagat gcccgccgtt ccgtcgccgt ccgcgaaggg gtaccgcaga
     2521 gacgtcagcg cgtcgtccat gtcttcgtac acgacctcga ggtcggagat cgcggtgtgg
     2581 tggtacgggc accagttgac gatgcggttg tcgcggtaaa tccacccccg cccccacaa
//
LOCUS       DBNGNK010000339         3748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_488692,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000339 DBNGNK010000000
VERSION     DBNGNK010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_488692"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="AC150V_10010"
     CDS             complement(<1..623)
                     /locus_tag="AC150V_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HIR3763292.1"
                     /translation="MTEPQPSPLRDVEQYAERFLASLADRAVGPQASVAELRARIGGP
                     MPATGRDARTVVRELVEALEPGVVASAGPRYFGFVTGGALPAALAADWITSVWDQNSF
                     SAAASPAAAAVEDVAGAWIAELLGIPRGSSFGFVTGTQAANLVALAAARDEMLRRAGW
                     DPGDGLAGAPQVQVIVGEARHVTVDRALRVLGIGRAGLRVAEADEHGR"
     gene            complement(620..1084)
                     /locus_tag="AC150V_10015"
     CDS             complement(620..1084)
                     /locus_tag="AC150V_10015"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR3763293.1"
                     /translation="MHTEPRIRVSAVLRWHGRILLIRHEKGDKEHWLLPGGGVKGGES
                     LTDALLRELAEETGLVGRGDELPVEGPVAIVDSISPGHLPSSKHVVHIIFAGDLAGSL
                     TQVVSQDDAVRGHRLFTPDELDSIAMHPPIQRFLRRWQPGDPCVYLGALWAR"
     gene            complement(1084..1524)
                     /gene="dut"
                     /locus_tag="AC150V_10020"
     CDS             complement(1084..1524)
                     /gene="dut"
                     /locus_tag="AC150V_10020"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019238587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR3763294.1"
                     /translation="MIELAVTRLRDEAVLPHSAYEGDAGLDLVACDRVKLEPGERRVV
                     GTGLAVAIPPGYAGFIQPRSGLAARHGIGVVNSPGLVDSGYRGELKVVLVNTDELESF
                     SVEPGMRIAQLVVLPVPAVAVVEVDELPESAERGVRGFGSSTHR"
     gene            complement(1521..2837)
                     /gene="hflX"
                     /locus_tag="AC150V_10025"
     CDS             complement(1521..2837)
                     /gene="hflX"
                     /locus_tag="AC150V_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR3763295.1"
                     /translation="MTTRQPDPAPGAEPEQGLIVAVLPKGADDDQELAELRDLARTAG
                     VEPVGELVQHRQHPDPRSYVGKGKLVELKDVYAESGAEVLLVDDEIDPTQQRALENAL
                     SARVVDRTQLILDIFAQHARSADGKLQVELAQLEYNLPRMRGMWQHLERLGGGVGTRG
                     PGETQLETDRRLANRRVSLLKRRLRQLAGQRATRRKERSRSITPTVALAGYTNVGKST
                     LLNALTGAGVSVNDQLFETLDPTTRGFDYEGRRYLVTDTVGFIRRLPTQLVEGFAATL
                     EETLVADLVLHVADASLAADRLQETISAVETVLHEIGADALQVELVLNKIDQVDALGR
                     RRLANRFPDAVQVSADTGEGLEELRGRVAELFANRFEEVRLLVPYADGAALSELYALG
                     APIDERTDTDDGVQIRARLPRHELARFAPYLVAGSAVEEQERRSAP"
     gene            2869..>3748
                     /locus_tag="AC150V_10030"
     CDS             2869..>3748
                     /locus_tag="AC150V_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3763296.1"
                     /translation="MHTADRLAARTLELVDIPSESRNEAAIRTHVRSLAGTALPEVFA
                     GEDAFLYAPERREGLPLVILAGHYDTVPAQGNVPGWLTDGAVHGCGASDMKGGLAVGL
                     ELVRDFAAAPPDRHDVGLIVFGKEELPPQFNPLPELFQGCELVHEAALVILLEPTDGQ
                     VHAGCLGNVNASVSFHGTSGHSARPWLAENAIEHAIEGLAPIVAHERREAVVGGLPFH
                     EVVSVTRLQAGIADNVIPDLAVAMLNYRYAPDRTPEEAEAHLRELIPPDAEIVIEGHS
                     PCGAVVTDTPLVTELVA"
BASE COUNT          571 a         1301 c         1283 g          593 t
ORIGIN      
        1 cgcccgtgct cgtcggcctc tgcgacgcgg aggccggcac ggccaatacc gagcacacgg
       61 agggcgcgat cgaccgtgac gtgcctggct tctcccacga tgacctggac ctgcggggcg
      121 cctgcgaggc cgtcgccggg atcccagccg gcgcgcctca gcatctcgtc ccgagcggcg
      181 gccagagcca cgagattcgc tgcctgtgtc ccggtcacga acccgaaaga cgatccgcgc
      241 gggatgccga gcagctcggc gatccaggct cccgcgacgt cctcgacggc cgccgccgcc
      301 ggcgacgcag cggccgagaa cgagttctga tcccatacgg acgtgatcca gtcggccgca
      361 agcgcggccg gcagcgctcc gccggtcacg aagccgaagt agcgcgggcc cgcgctggcc
      421 accactccag gctcgagtgc ctcgaccagc tcgcggacga ccgttcgtgc atcgcgaccg
      481 gtcgcgggca tcgggccgcc gatccgcgcg cgcagctcgg ccacgcttgc ctgcggcccc
      541 acggcacgat cggcgagaga ggcgagaaat cgctccgcgt actgctcgac gtcgcggagc
      601 gggctcggct ggggttccgt catcgcgccc agagcgcgcc gaggtagacg cacggatcgc
      661 cgggctgcca gcggcgtagg aaccgttgaa tcggggggtg catagcgatc gagtccagct
      721 cgtccggggt gaagagccgg tggccgcgca ccgcgtcgtc ctgggatacg acctgcgtca
      781 gcgatcccgc gaggtcgccg gcgaagatga tgtgtacgac gtgcttcgac gacggcaggt
      841 gcccgggcga gatggagtcg acgatcgcca ccgggccctc gaccggcagc tcgtcgcccc
      901 tcccgacgag cccggtctcc tccgccagct cgcgcaggag cgcatcggtg agactctcgc
      961 cccctttgac gcctccgccg ggcagtagcc agtgttcttt gtcgcctttc tcgtgcctga
     1021 tcaggagtat ccgtccgtgc cagcggagca ccgctgacac ccggattcgt ggttcggtat
     1081 gcatcagcga tgggtggacg agccgaagcc gcgcactcct cgctcggcgc tttccggcag
     1141 ctcgtcgact tccacgacgg cgacggcggg aacgggcagc acgacgagct gcgcgatccg
     1201 catcccaggc tcgacgctga acgattcgag ctcgtccgtg ttcacgagga cgaccttgag
     1261 ctcgccgcga tagccggagt cgacaaggcc gggagagttg acgaccccga tgccatgccg
     1321 ggcggcaagg ccagaccgtg gctggatgaa cccggcgtag cctggcggta tcgcaacggc
     1381 gaggccagtg ccgacgaccc ttcgctctcc cggctccagc ttcacgcggt cgcaggcgac
     1441 gagatcgagg cccgcgtctc cctcgtacgc ggagtgtggc agcacggctt cgtcacggag
     1501 ccgtgtgacc gccagctcga tcacggagcc gaccgtcgct cttgctcctc gaccgccgag
     1561 cccgcaacca gatagggagc gaagcgcgcg agctcgtgac ggggaagcct cgctcgaatc
     1621 tggacgccgt cgtcggtatc ggtgcgctcg tcgatcgggg cgccgagcgc gtagagctcc
     1681 gaaagggccg ccccatccgc gtagggcacg agcagtcgta cttcctcgaa ccgattggcg
     1741 aacagctcgg cgacccggcc gcgcagctcc tcgagccctt cgccggtgtc ggccgagacc
     1801 tggacggcgt cggggaaccg attcgcgagc cgtcgccggc cgagcgcgtc gacttgatcg
     1861 atcttgttga gcacgagctc cacctggagt gcgtccgccc cgatctcgtg cagaacggtc
     1921 tcgactgccg aaatcgtctc ctggagccga tccgccgcca gggaggcatc ggcgacatgg
     1981 aggacgagat ccgcgaccag ggtctcctcg agagtggctg cgaacccttc gacgagctgc
     2041 gtcggcagcc gccggatgaa gcccaccgtg tccgtgacga gatagcggcg cccctcgtag
     2101 tcgaagccac gtgtggtcgg gtcgagcgtc tcgaagagct ggtcgttgac cgagaccccc
     2161 gcaccggtca acgcgttcag cagagtcgac ttgccgacgt tcgtgtagcc ggcgagcgcg
     2221 accgtcgggg tgatcgaacg gctgcgctcc ttccggcgcg tggctcgttg tcccgccagc
     2281 tggcgcagac gtcgcttgag tagcgaaaca cgcctgttgg cgagccgccg gtctgtctcg
     2341 agctgcgtct cgcccgggcc tctggtgccg acgccgcccc cgaggcgctc caggtgctgc
     2401 cacatgcccc gcatccgcgg caggttgtat tcgagctggg caagctccac ttgcagcttc
     2461 ccgtcggcgc tccgtgcgtg ctgggcgaag atgtcgagaa tgagctgcgt tcggtcgacc
     2521 actcgtgctg acagggcatt ctcgagcgcc cgctgctggg tcgggtcgat ctcgtcgtcg
     2581 acgaggagca cctcggcccc cgactcggcg taaacgtcct tcaactccac gagcttgcct
     2641 tttccgacat agcttcgggg atcgggatgc tgccggtgct gtacgagctc acctacgggc
     2701 tcgacgccgg cggtgcgcgc cagatcgcga agctcggcaa gctcctggtc gtcgtcggcg
     2761 cctttcggca gcacggcgac gatgaggccc tgctccggct cggcgccggg tgcagggtcg
     2821 ggctgacgcg tggtcacccg gcggattatg cccgggtaag gtccggggat gcataccgcc
     2881 gaccggctcg ccgcccgcac gctcgagctt gtcgacatcc cctccgagag caggaacgag
     2941 gccgcgattc gcacgcatgt acgttcgctt gcgggcaccg cgctgcccga ggtcttcgcg
     3001 ggtgaggacg cgtttctgta tgcgccagaa cggcgagaag gccttccact ggtgattctc
     3061 gccggccatt acgacaccgt tcccgcccaa gggaacgtcc ccggatggtt gacggacggt
     3121 gcggtccacg gatgcggcgc gagcgacatg aagggcggac ttgcggtcgg gctcgagctc
     3181 gttcgcgact tcgctgccgc tccacctgat cggcacgacg tcggcctcat cgtcttcggc
     3241 aaggaggagc tcccgcccca gttcaatcct ctcccggagc tgttccaagg ctgtgagctc
     3301 gtccacgagg ccgcgctcgt catcctgctc gagcccacgg acgggcaggt tcacgcgggt
     3361 tgtctcggca acgtgaacgc gagcgtctcg ttccacggca cgagcgggca ctccgcgagg
     3421 ccgtggctcg cggagaacgc gatcgagcat gcgatcgagg gcttggcacc gatcgtggcg
     3481 cacgagcgcc gcgaagcggt ggtcggcggt cttccgtttc acgaggtcgt gagcgttacg
     3541 cggctgcagg cgggcatcgc cgacaacgtc atccccgatc tcgcggtcgc gatgctcaac
     3601 taccggtacg caccggaccg cacacccgag gaggcggagg cgcatctgcg ggagctcata
     3661 ccgccggacg ccgagatcgt catcgagggg cactccccgt gcggcgcggt tgtcaccgac
     3721 acgccgctcg tcaccgagct cgtcgcgg
//
LOCUS       DBNGNK010000340         5867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1420286,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000340 DBNGNK010000000
VERSION     DBNGNK010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1420286"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1988
                     /locus_tag="AC150V_10035"
     CDS             <1..1988
                     /locus_tag="AC150V_10035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3763297.1"
                     /translation="KIESRLRVLRHGSIVPALNAVAALRHAPRMDSAELEVLHAAAVE
                     GDGAARLLALHALAAQPDEASGAILLEGLYSDDPGRRQHAAWSLGDRAPEGAAVVGLL
                     SSLGRGGFEGMLAQLTLERWGAQEACLVSEALLGALRDEPAGPSRTRINETLGLMPEA
                     SASSERGLLAGTPLPTPSWSPRTSNGGLRVAQVLLQGRLDGRLEGAGAGDGGGLATLL
                     VSLSRSLAARGDVSEVVTFARAFVDDSLPDLYSELREPIGSGGSIERLRFGPHRYLGA
                     ASHWPYRVEIERELERAVHRVGGIDVAHLRFADAGTFAAARVFTRLGIPIVFTLAPDP
                     HAVLRDAESAGELSRESFPAADLDQHFVFRAWLVDWMVRNASALALLPRPSAEKELRT
                     LLGQPFERVEKARLRTIPEGIAVTESRAAESEIEAVAESAPLPASARDLADALVALPA
                     ERRGLPLLLSVGRFHRIKGLPLLVDTWAGDDELFARFNLVVVGGNLDRPTDEERAVLT
                     DIEEACRQRPRARVGLVMLGHRPNADVARLLQVTRLGLPGVAGERGVYVCPSRKEEFG
                     LAVLEAMAAGLPVVAPMAGGPPTYIDHGRNGFLADTTTAAGLAAGLHRAAAARFDDSL
                     SARARRLVADRFTIDAMAEELSSFYRDTAVTAEREAA"
     gene            1985..3343
                     /locus_tag="AC150V_10040"
     CDS             1985..3343
                     /locus_tag="AC150V_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3763298.1"
                     /translation="MTTLVVSPDYASHYLPLSAVASALADRGHDVVFATGPGLRDRVQ
                     ADGFEHAELSLGAGRNPGLARSNHDPGLSGFLDATKRGMVGTLTYQAEQRGRDLLWEP
                     ERVAERLSELVAELRPSFVVSDQLAYGASLGLRAIGQPYAAFLPGHPCQLPPRTEPFG
                     YPARRPTEIEATPEELARLRELCGKTAADFTATFNRTLHSLAPNATPVTDAFAAGSPW
                     LTLVNYPAELARRRRFPHRTTLVGSCVRRETGDAELDSLDRGDTPPRAYVALGSFLSA
                     RTDVLERVAAGLRLAGLEAVIATGVADPAVLGPIPSRWVVRPYVPQVAALRACDVVVC
                     HGGNNTVTEALAAGLPVVSLPFSTDQFAGAADLHEAGLGATVDPNAVTPATIRSAVER
                     LLEPPVRARAASIGQRLRGRPGARLSALLVERALPGTRTRARTRRAYPTARRLARAAR
                     SS"
     gene            3353..4828
                     /locus_tag="AC150V_10045"
     CDS             3353..4828
                     /locus_tag="AC150V_10045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3763299.1"
                     /translation="MSRDSLTRSRLTLLATALGSSLAFLDATVVVVALPRMEADLGLG
                     LAGQQWVVLGYSLALSSFLMVGGAVGDRAGLRRTFIVGVVAFALASLLCAVAPSPALL
                     IAGRFVQGVGGAALTTTSLALLRVVWADESGRAIGLWTSLTAVATVIGPPLGGVLVEF
                     ASWRWIFLINLPLAAVVVVLAVAGRTEREEPDARGRLDLVGSALAAAGLAGVTFALVE
                     ARERGLGEVLAAGLIGLAALVALVFWTLRAPAPLVPPRLLRRPGLAAANSVTFVVYAA
                     LGVNFLFLPIFLQYLGLSPTVSGLAFIPPSAALILLAPRFGRVSDRVGPRLPVAVGAG
                     TIGISALLLLPISAAEDVWVWGVASILLFSVGIAAIVAPITAAALSPAPANLAGIASG
                     LNQTVARVGGVLSVAAVGALAGWIYSRSGGLAATPFDPSTPEAARGAGVDAFRALVLV
                     SAGLAFAGAALALALLVGRRPVRDVADEVAVCPCPQAGPSA"
     gene            complement(4830..5390)
                     /locus_tag="AC150V_10050"
     CDS             complement(4830..5390)
                     /locus_tag="AC150V_10050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR3763300.1"
                     /translation="MNVSADLDSRFRDAAARAGLLDVGYDVIDSPIGPLLVAASDEGL
                     VRISFDSDPEHHLDQLARTAGPRVLRAPRAVADAHRELDDYFEGRRQAFELTLDLRGT
                     TEFTRNVLDELALVPYGETATYAELARRAGRPKAARAVGMVMNRNPIPIVLPCHRVVG
                     SSGSLVGYAGGLDVKEFLLRLEGVVR"
     gene            complement(5387..>5867)
                     /locus_tag="AC150V_10055"
     CDS             complement(5387..>5867)
                     /locus_tag="AC150V_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3763301.1"
                     /translation="RRLRGMMARVRVPPFELFYAEHRGAVLRLLRQRLGFEHADDAFQ
                     ETFLRALRAYDRLEHGEHLRAWVLTIAGRVAIDTWRRLPETAELPELAHHDARPAYEE
                     LGDLTGELPPTEQAAVVLRYGYDLDYEQIAAALSSSPDAARQAASSGVRRLRRRSAT"
BASE COUNT          701 a         2126 c         2085 g          955 t
ORIGIN      
        1 ccaagatcga gagtcggttg cgggttcttc ggcacggcag catcgtcccg gcgctgaacg
       61 ccgtcgcggc cctgcggcac gcgccgcgca tggacagcgc cgagctcgag gtgctccacg
      121 ccgccgctgt cgagggggac ggcgcggcgc ggctgctcgc tctccatgcg ctcgcagccc
      181 agcccgacga ggccagtggc gcgatcctgc tcgaggggct gtactcggac gatcccggcc
      241 gtcggcaaca tgccgcgtgg tcgctcggcg atcgtgcgcc ggagggggcc gcagtcgtgg
      301 gcctgctgag ctcgctgggt cgcggcgggt tcgagggcat gctcgcccag ctgacgctcg
      361 agcgctgggg cgcgcaggag gcctgcctgg tctccgaagc gctgctcggc gccctgcgcg
      421 acgagcccgc gggcccgtcg cgcacccgaa tcaacgagac gctggggctc atgccggaag
      481 cctcggcgtc gtcggaacgt gggctcctcg cggggactcc gctgccgacg cccagttggt
      541 cgccccgcac ctcgaacgga ggcctgcgtg tcgcccaggt cctgctccag ggtcgcctcg
      601 acgggcgact ggagggcgca ggcgccggag acgggggcgg gctcgccacc ctgctggtca
      661 gtctctcccg gtcgcttgcg gcgcgcggcg acgtgtccga ggtcgtgacc ttcgcgcggg
      721 cgttcgtcga cgactcgctc cccgacctct actcggaact gcgcgagccg atcggaagcg
      781 gcggctcgat cgagcggctc cgcttcgggc cgcaccgcta tctcggtgcc gcctcccatt
      841 ggccgtaccg ggtcgagatc gaacgcgagc tcgagcgtgc cgtccaccgt gtggggggga
      901 tcgacgtcgc acacctgcgt ttcgcggacg cgggtacgtt cgcggcggcg cgcgtcttca
      961 cgcggctcgg cattccgatc gtcttcaccc tcgcgcccga tccgcacgcg gtgctccggg
     1021 atgccgagtc ggccggtgag ctgagccgtg agtcgttccc ggccgccgac ctcgaccagc
     1081 acttcgtctt ccgggcctgg ctcgtcgact ggatggtgcg gaacgcttcc gcgctcgccc
     1141 ttctcccgcg tcctagcgca gagaaggagc tgaggacgct gctcggacag ccgttcgagc
     1201 gcgtcgagaa ggcgcggctt cgcactatcc cggagggcat cgccgtcacc gagtcgcggg
     1261 ccgcggagag cgagatcgaa gccgtcgccg agtctgcgcc gcttcccgcc tccgcgcgcg
     1321 atctggcgga tgcgctcgtc gcgctgcccg ccgagcgtcg cggcctgccg ctcctgctgt
     1381 cggtcggccg gtttcaccgg atcaagggac tgccgcttct cgtcgacacc tgggcgggtg
     1441 acgacgagct gttcgcgcgc ttcaacctcg tcgtcgtcgg cggcaacctc gatcggccca
     1501 ccgacgagga gcgcgccgtt ctgacggaca tcgaagaagc ctgtcggcag cgtccacggg
     1561 cgcgcgtagg gctggtcatg ctcggtcatc gcccgaacgc cgacgtcgcg cgcctgctgc
     1621 aggtgacgcg cctcggtctc cccggcgtcg ccggggagcg cggtgtctac gtgtgcccca
     1681 gtcgcaagga ggagttcggc ctcgccgtgc tggaggccat ggccgcgggt cttcccgtcg
     1741 tcgccccgat ggcgggtggt ccccccacct acatcgacca cggccgcaac ggtttcctcg
     1801 cggatacgac aacggcggca gggctggcgg ctgggctgca ccgggcggca gctgctcgct
     1861 tcgacgactc cctctcggcc cgcgcgagac ggctcgtggc ggaccgtttc acgatcgacg
     1921 ccatggcgga ggagctctct tccttctatc gcgacaccgc cgtcacggcc gagcgagagg
     1981 ccgcgtgacg accctcgtcg tcagcccgga ctacgcatcg cactaccttc cgctgtcggc
     2041 cgtggcatcg gcgctcgctg accgtggcca cgacgtcgtc ttcgccaccg ggcccggcct
     2101 gcgagaccgc gtgcaggcag acggattcga gcacgcggag ctctccctgg gagccggtcg
     2161 caacccgggt ctggctcgca gcaaccatga tcccggcctg agcggcttcc tggacgccac
     2221 caagcggggc atggtcggca cgctcaccta tcaggccgag cagcgagggc gcgacctgct
     2281 gtgggagccg gagcgcgtcg cggagcggct gtcggagctc gtcgcggagc tccggccgag
     2341 cttcgtcgtg tccgatcagc tcgcctacgg agccagtctg ggtctgcgcg cgatcggcca
     2401 gccgtacgcc gccttcctgc ccgggcaccc gtgccagctc ccgccccgaa ccgagccgtt
     2461 cggctacccg gcgcggcggc cgacggagat cgaagcgact cccgaggaac tcgcgcggct
     2521 tcgcgagctg tgcggcaaga cggcggcgga cttcaccgcg acgttcaacc gcacgctgca
     2581 cagcctcgct ccgaatgcga ctcccgtgac ggacgcgttc gccgcggggt cgccgtggct
     2641 gaccctcgtc aactatcccg ccgagctcgc gcggaggcga cggtttcccc accggacgac
     2701 gcttgtcggc tcgtgtgttc gacgcgagac tggcgacgca gagctcgact cgctcgatcg
     2761 aggcgatacg ccgccacgcg cgtacgtcgc gcttgggagc ttcctttcgg cgcggacgga
     2821 cgtgctggag cgcgttgccg caggcctgcg gctcgctggc ctagaggcgg tcatcgccac
     2881 cggcgtggca gatcctgcgg ttctggggcc gattccgtcg aggtgggtcg tccggccgta
     2941 cgtcccgcag gtcgccgcgc tgcgtgcctg cgacgtcgtc gtctgccacg ggggcaacaa
     3001 caccgtcacc gaagctctcg cggcgggcct tcccgttgtc tcgctgccgt tctccaccga
     3061 tcagttcgcg ggggcggcag acctccatga agccgggctc ggtgcgaccg tcgaccccaa
     3121 cgccgtcacc ccggccacca tccggagcgc tgtcgagcgg ctgctcgagc ccccggtgcg
     3181 agcgagagcg gcgtcgatcg ggcagcgatt gcggggccgg ccgggcgccc gactctcggc
     3241 gctgctcgtc gagcgggccc tgcccggtac gagaacacgc gctcgcacgc gccgcgcgta
     3301 tccgaccgca cggcgtctcg cgagggcggc tcggtcctcc tagcctgggg ctatgtcccg
     3361 ggactcgctc acgcgcagcc gcctcacact gctcgcgacc gcgctcggct cgtcgctcgc
     3421 gttcctcgac gctacggtcg tcgtcgtcgc gcttccgcgc atggaggccg acctcggact
     3481 cggcctcgca gggcagcagt gggtcgtgct cggctactcg ctcgcgttgt cgtcgttcct
     3541 gatggtcggc ggtgcggtcg gcgatcgagc gggcctgcgc cggacgttca tcgtcggcgt
     3601 cgtcgctttt gctctcgcct cgctcctctg cgcagtcgcg ccgtcacccg ccctgctgat
     3661 cgccggaagg ttcgtgcaag gagtgggagg ggcggccctg acgacgacca gcctggcgct
     3721 cctgcgcgtc gtctgggccg acgaatcggg ccgggcgatc ggcctgtgga cgtcgctcac
     3781 tgccgtcgcc accgtcatcg ggccgccgct gggcggcgtg ctcgtcgaat tcgcctcgtg
     3841 gcgctggatc ttcctcatca acctgccgct cgccgctgtc gtcgtcgtgc tcgccgtcgc
     3901 gggccggacg gagcgcgagg aaccagatgc tcgcggccgc ctcgaccttg tcggctcggc
     3961 cctggcggcg gcggggctcg cgggcgtgac gttcgctctc gtcgaggcgc gcgagcgcgg
     4021 gctcggagag gtgctcgccg ccggactgat cggccttgcg gcgctggtcg cgctcgtctt
     4081 ctggacgctg cgcgcgcctg cgccactcgt gccgccccgc ctgttgcgcc ggccggggtt
     4141 ggctgcggcg aacagcgtga ccttcgttgt ctacgcggcc ctcggcgtca acttcctctt
     4201 cctgccgata ttcctgcagt acctcggtct ctcgccgacg gtgtccgggc tcgccttcat
     4261 cccgccgagc gccgcgctga tcctgctcgc gccgcgcttc ggacgcgtct cggatcgagt
     4321 cgggcctcgc cttcctgtgg ctgtgggtgc cgggacgatc gggatcagcg cgctgctcct
     4381 gctcccgatc tcggcggccg aggacgtctg ggtgtggggt gtcgcaagca tcctcctctt
     4441 ctcggtcggg atcgcggcga tcgtcgctcc gatcaccgcc gccgctctct ctcccgctcc
     4501 cgcgaacctt gcggggatcg cgtcgggcct caaccagacc gtcgcccgag tgggcggcgt
     4561 cctctccgtg gctgccgtcg gcgccctcgc gggatggatc tactcgcgct cgggcggact
     4621 cgccgcgacg ccgttcgacc cgagtacgcc ggaggccgcg aggggcgcgg gggtcgacgc
     4681 cttcagagct ctcgtgctcg tcagcgcagg actcgccttc gccggtgcgg cgctggcgct
     4741 ggcactgctc gtgggtcgga gacccgtgcg cgacgtagcc gacgaggtag ccgtgtgccc
     4801 gtgccctcag gcgggcccga gcgcgtgagc tagcggacga cgccctccag cctgagcaag
     4861 aactccttga cgtcgagtcc gcccgcgtag ccgaccaggc tgcctgacga gccgaccacc
     4921 cgatggcagg ggagcacgat cgggatcggg ttgcggttca tgaccatccc cacggcccgc
     4981 gccgccttcg ggcggccggc ccgtcgagcg agttccgcgt acgtggccgt ctctccgtag
     5041 ggaaccagcg ccagctcgtc cagaacgttt ctcgtgaact ccgtcgttcc ccgcaggtcg
     5101 agcgtgagct cgaacgcctg gcgtcgcccc tcgaagtagt cgtcgagctc gcggtgcgcg
     5161 tcggcgacgg cgcgtggcgc gcgcagcaca cgcggcccgg cggtgcgggc gagctggtcg
     5221 aggtgatgct cggggtcgga gtcgaaggag atccgaacga ggccctcgtc gctggccgcg
     5281 acgagcagcg ggccgatcgg cgagtcgatc acgtcgtacc cgacgtcgag cagcccggcg
     5341 cgcgccgccg catcgcgaaa gcgcgagtcg agatcggcag agacgttcat gtcgcactcc
     5401 tccttcgaag gcggcggacc ccggacgacg ccgcctgtcg tgccgcgtct ggactcgacg
     5461 agagcgccgc cgcgatctgt tcgtagtcga ggtcgtagcc gtaacgcagg acgacggcgg
     5521 cctgctcggt cggcgggagt tcgcctgtca gatcgccgag ctcctcgtac gcgggtcgtg
     5581 cgtcgtggtg cgcaagctcg ggcagctcgg cggtctcggg cagtcgtcgc cacgtgtcga
     5641 tggcgacccg gccggcgatt gtgagcaccc acgcccgcag atgctcgccg tgctcgaggc
     5701 gatcgtaggc gcggagagcg cgcaggaagg tttcctgaaa cgcgtcgtcg gcgtgctcga
     5761 agccgagacg ttgccgcagg aggcgcagca ccgctccgcg atgctcggcg tagaacagct
     5821 cgaagggtgg gactctcacg cgtgccatca tccctctcaa ccgccgg
//
LOCUS       DBNGNK010000341         5941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_60471,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000341 DBNGNK010000000
VERSION     DBNGNK010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_60471"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="AC150V_10060"
     CDS             complement(<1..606)
                     /locus_tag="AC150V_10060"
                     /inference="COORDINATES: protein motif:HMM:NF017009.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE/PilB domain-containing protein"
                     /protein_id="HIR3763302.1"
                     /translation="MAQLRQNRLRTAGDTSVASRSDAAFGDGGFETIADLVAELVAAT
                     GLVPADRLAAARGRAGTGSLAQALADEGFAKPDGIARATARKHGLDLIDLSEERVSPD
                     AAELIPLQVLRRVVAIPVARIDDRLRVAVADPSNLHGMEELRLATRYSIELLIASREE
                     ISVELERLARQSEVVETQSYLDEIEVVADDEDDLEVEDGVSD"
     gene            complement(724..2082)
                     /locus_tag="AC150V_10065"
     CDS             complement(724..2082)
                     /locus_tag="AC150V_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763303.1"
                     /translation="MTRFRAIEQERGVAMIMVVMVGLLLTVLSLTIINLVRGESDRSA
                     QQIKRGASFQAAEAGIDDYVAKVVDDRLFYLHKVHEAESTRQEPLGSQTSAGSAWAYG
                     RQWTYPNGKDAWRQLPNGYEYNLQISPPDVGSQFIRIIATGRKAGSTTDVRSIETYIR
                     PSSLADFYRVVNGDVGWGSGATTNGKIYANGNVSHNGTAAASIYAENQISGSTTLING
                     AKKYDVDSSPTIRSQVKTPLNFSNFLTSFVDIERAAQIGGIHLDDPTKAAWKLVFNSN
                     GTVNIQACTQSGGNHVAQTSPNCGAATSYTVPANGAIYSAQNAIVSGNVKGRVTVASN
                     DNIIVADNIAYVQIGDDVLGLVAKNYVYIAWYVPSNFTWMASVLAQNGTWQGYPGCCS
                     PKKSLMTFTGSSATDDGGSFSGMFTNRNYSYEPALQYLSPPWFPTIDDAYTVLFFREL
                     PT"
     gene            complement(2079..2684)
                     /locus_tag="AC150V_10070"
     CDS             complement(2079..2684)
                     /locus_tag="AC150V_10070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PulJ/GspJ family protein"
                     /protein_id="HIR3763304.1"
                     /translation="MLSGRVRTLRRDGGFTLIEMLTAMVVLGFMFAAVAVVLSSMVRH
                     SDEVEDRSVLQSDARFTIDRLSVDLRQAYSGVDGVSPIESISPTGLTFLSPDRASPFH
                     LRRISYRLNNGKIERAMATSTDTDGAPWNIPPLGAWVKQVDSVTNAAPFTYYDSAGAV
                     TTVAADVSTVKVDLDLVTNSSPTRKLRYTTSVTVRSDVETS"
     gene            complement(2678..4270)
                     /locus_tag="AC150V_10075"
     CDS             complement(2678..4270)
                     /locus_tag="AC150V_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763305.1"
                     /translation="MSHLSLKLRRTRPATDQSGFTLVEAVMALVIFVGVAASLAGLLT
                     SAISATKVARERTVAEQAAMEQIESIRRMPYDSVGVVSGNPPGTIPATKAISVRGLQG
                     TAKTQVSFVADPTPTSYATSANYKRVTVVVTRNGDSKELARQVTYVSPPAQAPYGGIN
                     KAIVQAEVQDYALNTPIANATVNLLTGPSAPRTDTTDAVGKVTFPDLTPNPASGPQAY
                     YDLTASLTGYELFLDPIAQHFWVAPGQTVNPSLQLFKPATIEIVTKNAGGSPYTGPVT
                     VDIYSELTGESETITVTGGYKAVTSLVGKSLVPAVNYTVGGFAAPGPLCAPASTSYVP
                     DNYPTDLSTTFTLTLAPCPSGTLVAQVTSGGSPALGANVTVSDGPYSISQSATTDSTG
                     YASFTVPAGAGYNVTATLGAQNAQANNITVPVSGTVNVPLALSSPTASLTVHVTRNGS
                     NRSSATVELAGGPQSVNITHPSLTDGGGNVTFTNVPVGSGYTVTAYRCNHGAPDRGIV
                     TGATVSAPSTTVDVAFSGGNTC"
     gene            complement(4307..5050)
                     /locus_tag="AC150V_10080"
     CDS             complement(4307..5050)
                     /locus_tag="AC150V_10080"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR3763306.1"
                     /translation="MTSAALALAPALALGSFLNVVVARVPERKSLVAPRSACPNCESE
                     ISWRDNIPVLSYLLLRGRCRHCRSPITPLYPAVEIATAALIVGCVAVFGLTAYGALAA
                     AFCAVLVVLSAIDARQRIVPNRIVVPAAVVVLAARTAIDPSLEWLLAAAGAFLFFFLA
                     ALAYPAGLGMGDVKFAFLLGAMLGVSVISGLLIGLIAALLPSVVLLARHGSAARKMGI
                     PLVPFLSFGAIVALFAGNALTGGYTGLFS"
     gene            complement(5047..>5941)
                     /locus_tag="AC150V_10085"
     CDS             complement(5047..>5941)
                     /locus_tag="AC150V_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR3763307.1"
                     /translation="DSNQNVTLEELNVPAPELSQFRRLLSFREGIVVLTGPTGSGKTT
                     TLATMIDHINRSRRQHIVTVEDPIEVLHPDRGCIVNQREVGLDTASFEQALRRVLRQD
                     PDIILIGELRDTETAHTALQAAESGHLVFSTLHTLDCSETIGRMIEFFPPEKQQQIRS
                     ILAGTLKGVVSQRLLPRADGGRVPAIEVMVANARIEDLIRENRPDEIEDAIDSGEFYE
                     MQTFAQSLISLVLDGAVDREVAANAATNKHDFLVALEAAAKRREEDDAETGQGAQTSK
                     LPSAGGLRTGPAESLEGVRLR"
BASE COUNT          923 a         1877 c         2153 g          988 t
ORIGIN      
        1 gtcggagacg ccgtcctcga cctcgagatc gtcctcgtcg tcggcgacga cttcgatctc
       61 gtcgaggtag gactgggtct cgacgacctc ggattggcgc gcgaggcgct cgagctcgac
      121 cgagatctcc tcgcggctcg cgatgagcag ctcgatcgag tacctggtcg caagccggag
      181 ctcttccatc ccgtgcaggt tgctcgggtc ggcgacggcg acgcggaggc gatcgtcgat
      241 ccgggcgacc gggattgcca cgacccggcg caatacctgg agcgggatca gctcggcggc
      301 gtcgggcgag acgcgctctt cggacaggtc gatcaggtcg aggccgtgct tgcgcgcggt
      361 agcgcgggcg atgccatcgg gcttggcgaa accctcgtcg gccagcgcct gcgccagcga
      421 gcccgtaccg gcccgcccgc gcgccgctgc gaggcggtcg gccgggacga gcccggtggc
      481 ggcgacgagc tcggcgacca gatcggcgat ggtctcgaag ccaccgtctc cgaaggcggc
      541 gtcgctgcgc gaggcaacac tcgtgtcgcc cgcggttcgg agcctgtttt gcctcagctg
      601 cgccatctat ccctcaggcg aggtatcggc atcgcgcggc ccggccctta ggccctgaca
      661 ggccctgccg agaggtgacg cgagccccac cgcattcgaa ggccgccgac ccacgcgtcc
      721 ggatcaggtc ggcagctcgc ggaagaacag gacggtgtac gcgtcgtcga tcgtcgggaa
      781 ccacggcggc gagaggtact gcagcgcggg ctcgtacgag tagttgcggt tcgtgaacat
      841 tcccgagaag ctaccgccgt cgtctgtcgc cgacgagcct gtgaaggtca tcagcgactt
      901 cttcgggctg cagcaccccg ggtagccctg ccacgtgccg ttctgggcga gcacgctcgc
      961 catccaggtg aagttgctcg gcacgtacca ggcgatgtag acgtagttct tcgccacgag
     1021 ccccagcacg tcgtcgccga tctgcacgta cgcgatgttg tcggcgacga tgatgttgtc
     1081 gttcgaggcc acggtgacgc ggcccttcac attgccggag acgatcgcgt tctgggctga
     1141 gtagatggcc ccgttcgccg ggacggtgta gctcgtcgcc gcaccgcagt tcggcgaggt
     1201 ctgcgcgacg tgattgccgc ccgactgcgt acaggcctgg atgttgaccg tgccgttgga
     1261 gttgaagacg agcttccacg ccgccttcgt cgggtcgtcg aggtggattc cgccgatctg
     1321 tgccgctcgc tcgatgtcca cgaacgacgt gaggaagttg ctgaagttga gcggcgtctt
     1381 cacctggctg cggatggtcg ggctcgagtc gacgtcgtac ttcttcgcac cgttgatcag
     1441 cgtcgtggag ccgctgatct ggttctcggc gtagatgctc gcggcggcgg tgccgttatg
     1501 gctgacgttg ccgttcgcgt agatcttccc gttcgtcgtc gcgccggagc cccagcccac
     1561 gtcgccgttc acgacgcggt agaagtcggc cagcgacgac gggcggatgt acgtctcgat
     1621 cgaacggacg tcggtcgtgc tcccggcctt acgccccgtg gcgatgatgc ggatgaactg
     1681 agagccgacg tccgggggcg agatctggag gttgtactcg tagccgttcg ggagctgccg
     1741 ccaggcgtcc ttcccgttcg gatacgtcca ctgccggccg taggcccacg ccgagcctgc
     1801 gctcgtctgg gagccgagcg gctcctgccg cgtcgactcg gcctcgtgca ccttgtggag
     1861 gtagaagagc cggtcgtcca cgaccttcgc cacgtagtcg tcgatgcccg cctctgccgc
     1921 ctggaacgac gcgcctcgct tgatctgctg cgcgctgcgg tcggactcgc cgcggacgag
     1981 gttgatgatc gtgagcgaga gcaccgtcag gagcagtccg accatgacga ccatgatcat
     2041 cgcgacgccc cgctcttgct cgatcgcgcg gaagcgggtc acgacgtctc cacgtcggag
     2101 cgcacggtga cgctcgtggt gtaacgaagc ttgcgggtcg gcgagctgtt ggtgacgaga
     2161 tcgaggtcga ccttcaccgt gctcacgtcc gccgcgacag tcgtcacggc gccggcggag
     2221 tcgtagtagg tgaacggcgc ggcgttggtg accgagtcga cctgcttcac ccaagcgccg
     2281 agcggcggga tgttccacgg agccccgtcc gtatcggtgc tcgtggccat tgcgcgctcg
     2341 atcttgccgt tgttgagccg gtaggagatc cggcgcaggt ggaacgggct cgctcgatca
     2401 ggggacagga aggtgagccc cgtcgggctg atgctctcga tcggcgacac gccgtcgacg
     2461 ccggaatacg cctggcggag gtcgaccgag agccggtcga tagtgaagcg ggcgtccgac
     2521 tggaggacac tgcgatcctc gacctcgtcg ctgtggcgga ccatcgagct caggacgacc
     2581 gccacggcgg cgaacatgaa gccgagcacg accatcgccg tcagcatctc gatgagcgtg
     2641 aagccgccgt ctcggcgcaa cgtgcggacg cgcccgctca gcacgtgttg cctccggaga
     2701 acgcgacgtc cacggtggtc gacggcgccg aaacggtcgc gccggtcacg attccgcggt
     2761 ccggagcgcc gtggttgcag cgataggccg tgaccgtgta tccggagccg accgggacgt
     2821 tcgtgaacgt gacgttcccg ccaccgtcgg tcaggctcgg gtgagtgatg ttcacggact
     2881 gcggcccacc tgccagctcg accgtggcgc tcgaccggtt gctcccgtta cgcgtgacgt
     2941 ggacggtcag ggaggcggtc ggcgacgaaa gggcgagcgg gacgttcacg gtgccgctca
     3001 caggcacggt gatgttgttg gcctgcgcgt tctgggcgcc gagcgtcgcc gtcacgttgt
     3061 agccggcgcc ggccggcacc gtgaaggagg cgtaaccggt cgagtcggtc gtcgcggact
     3121 gcgagatcga gtaggggccg tcggagacgg tgacgttcgc gccgagcgca ggcgagccgc
     3181 ccgacgtgac ctgcgccacg agcgtgcccg aggggcacgg agccaacgtg agcgtgaacg
     3241 tcgtcgagag atccgtcgga tagttgtcgg gcacgtagct ggtggaggcg ggcgcgcaga
     3301 gcggcccggg agccgcgaag ccgccgaccg tgtagttgac ggcgggcacg agggacttgc
     3361 cgaccaggga ggtcacagcc ttgtagccgc ctgtcacggt gatggtctcg ctctcacccg
     3421 tgagctcgga gtagatgtcg accgtgaccg ggcccgtata ggggctgccg cccgcgttct
     3481 tggtcacgat ctcgatcgtc gccggcttga agagctgcag tgacgggttg accgtctgtc
     3541 ccggcgcaac ccagaagtgc tgcgcgatcg ggtcgaggaa gagctcgtag ccggtcaggc
     3601 tggcggtgag gtcgtagtaa gcctgtgggc cgctcgcggg gttgggcgtg agatccggga
     3661 acgttacttt cccgacggcg tccgtcgtgt cggtgcgcgg ggcgctgggg ccggtgagca
     3721 ggttcacggt ggcgttcgcg atcggagtgt tcagggcgta gtcctgaacc tctgcctgga
     3781 cgattgcctt gttgatgccg ccgtagggcg cctgtgcagg aggcgagacg tacgtgacct
     3841 ggcgcgcgag ctccttggag tcgccgttcc gtgtcacgac gaccgtgacg cgcttgtagt
     3901 tcgcggacgt cgcgtagctc gtcggggtcg ggtcggcgac gaacgacacc tgcgtcttgg
     3961 cggttccctg cagcccccgc acgctgatcg ccttcgtcgc cggaatcgtc cccggcgggt
     4021 tgccgctgac gacaccgacc gagtcgtacg gcatgcggcg gatcgactcg atctgctcca
     4081 tcgcggcctg ctccgcgacc gtgcgctccc gtgccacttt cgtggcgctg atcgcagacg
     4141 tgaggaggcc ggcaagggaa gccgcgacgc caacgaagat cacaagggcc atgaccgcct
     4201 cgaccagcgt gaatcccgac tgatcggtgg caggtcgcgt acgccgcagc ttgagggaga
     4261 gatggctcat gtcgcgtcgg tcactcgagg agccgtctca gaatggtcac gagaagaggc
     4321 ccgtgtatcc cccagtaagg gcattccccg cgaagagagc cacgattgcc ccgaatgaga
     4381 gaaacggcac gagcgggatg cccatcttgc gggcggcgga gccgtggcgg gcgagcagga
     4441 cgacgctggg aaggagcgcg gcgatgagcc cgatgagcag gccggagatg acgctcacgc
     4501 cgagcatggc gcccagcagg aaggcgaact tgacgtctcc catacccaga ccggccgggt
     4561 aggcgagggc ggcgaggaag aagaacagga aggcgcctgc ggccgcgagc agccactcga
     4621 ggctggggtc gatggccgtc cgtgcggcaa gcacgaccac cgccgcgggt acgacgatgc
     4681 ggttcgggac gattcgctga cgggcgtcga tggccgagag cacgacgagc acggcgcaga
     4741 aggcggccgc cagcgcgccg tacgcggtga gcccgaagac cgcgacgcac ccgacgatga
     4801 gggccgccgt cgcgatctcg acggccgggt agaggggagt gataggcgac cggcagtggc
     4861 ggcagcggcc gcggagcagc aggtacgaga gcacggggat gttgtcccgc cacgagatct
     4921 cgctctcgca gttggggcag gcggagcgcg gagcgacgag cgattttcgc tctggcaccc
     4981 gggcaacgac gacgttgagg aagctgccga gcgcgagggc cggcgccagc gcgagcgccg
     5041 ccgaggtcat cgcagacgga cgccttcgag tgactcggcg gggccggtgc gaagcccgcc
     5101 ggcggagggt agcttggacg tctgcgcgcc ttgtccggtc tccgcatcgt cttcctcgcg
     5161 tcgcttcgcg gcggcttcga gcgcgacgag gaaatcgtgc ttgttcgtcg cggcgttcgc
     5221 ggcgacctcg cggtcgaccg ccccgtcgag caccagcgag atgagcgact gggcgaacgt
     5281 ctgcatctcg tagaactcac ccgagtcgat tgcgtcttcg atctcgtccg gccgattctc
     5341 gcggatgaga tcctcgatgc gggcgttcgc gaccatcacc tcgatcgcgg gaactcggcc
     5401 gccgtctgcg cgcggcagta gccgctggct gacgacgccc ttgagcgtgc ccgcaaggat
     5461 cgagcgaatc tgctgctgct tctcgggcgg gaagaactcg atcatgcgac cgatggtctc
     5521 cgagcagtcc agcgtgtgca gcgtcgagaa gacgaggtgc ccggactccg ccgcctggag
     5581 cgccgtgtgg gcggtctcgg tgtcgcggag ctcgccgatc aggatgatgt ccgggtcctg
     5641 acggagaacg cgccgcagcg cctgctcgaa gctcgccgtg tcgaggccga cctcacgctg
     5701 gttgacgatg cagccgcgat cggggtgcag gacctcgatg gggtcctcca cggtgacgat
     5761 gtgctggcgc cgcgagcggt tgatgtggtc gatcatcgtc gcgagcgtcg tcgtcttccc
     5821 cgatccggtg ggcccggtga gcaccacgat gccctcacga aacgagagca gccgccggaa
     5881 ctgggaaagc tccggcgccg ggacgttcag ctcctcgagc gtgacgttct ggttcgaatc
     5941 g
//
LOCUS       DBNGNK010000342         3546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1851089,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000342 DBNGNK010000000
VERSION     DBNGNK010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1851089"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1994)
                     /gene="nuoL"
                     /locus_tag="AC150V_10090"
     CDS             complement(93..1994)
                     /gene="nuoL"
                     /locus_tag="AC150V_10090"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR3763308.1"
                     /translation="MIVGGWICLLAPLAGALLITVLGPLVSRRTAAFISIGTTFLAFA
                     GAMLAFVSMWARSPDERAGVSTAFTWLAAGNFEVGFSLLIDPLSIVMMLVVSGVGGLI
                     IWYSLGYMHGEDEERRYFAYMSLFVFAMLTLVEAGNFLFLLVGWGLVGLASYLLIGYY
                     HQRTEAVAAAKKAFVMNAIGDATMALAFFVLIWQVGTLDFGPVFEAAEGSDLSQTQLN
                     LVALGLLGGAVAKSAQIPLHTWLPDAMEGPTPVSALIHAATMVTAGVYLIARAHPIFE
                     AAPDIQALAAILGAVTLVFAGLVALVQQDIKRVIAYSTMSQIGYMFVGVGVGAYGFAM
                     YHLVTHAFFKALLFLGAGVVIHKLAGEQDIRKMGGLHRYMPFTHITFLIGALALIAIP
                     PLSGFWTKDAILASALATGSALGWFLYVMGLIGALLTGLYAFRLYWLVFRGEPSEFAL
                     EHVTETHGDGPREGPWSMLIPVGVLSVLATIGGFLDIPGAWEIFEEWVAQVAEPLELP
                     TVAEDYATSAVAVALGAIGIYVAWRAVSRGRDLVPWPQIEAVFERKFYADELYDALFY
                     RPAAMIAVRLRTDVETPVVEGSLTDIGEGAGEAASAVARAQTGYLRSYALVIALSVVV
                     LAIVFMAVR"
     gene            complement(1991..2299)
                     /gene="nuoK"
                     /locus_tag="AC150V_10095"
     CDS             complement(1991..2299)
                     /gene="nuoK"
                     /locus_tag="AC150V_10095"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR3763309.1"
                     /translation="MTGPGIPWYLAVAGILFATGTAGVLLRRSTLIVLLSLEIMLNSA
                     NLALVAFSRQFGAVDGQVFALGVMAVAAAEVTVGLGLIVAMARRRLPLDVDRFKELSG
                     "
     gene            complement(2296..2844)
                     /locus_tag="AC150V_10100"
     CDS             complement(2296..2844)
                     /locus_tag="AC150V_10100"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR3763310.1"
                     /translation="MGTFLVWLVWILAALGCIGAGVAVITFANPFYSALALIGNLASL
                     AVLFLLASAEFVAAAQVLVYAGAVMVMFLFVIAYLGGRADAPWAGGTRLFQVAAAGAA
                     AALLVVVVVAFAQWANDSLSDPADITRAFGSPAAIGELFLTDHLLAFEITSIILLVAA
                     IGGVVLGMSARDSAERDEEGER"
     gene            complement(2854..3348)
                     /gene="nuoI"
                     /locus_tag="AC150V_10105"
     CDS             complement(2854..3348)
                     /gene="nuoI"
                     /locus_tag="AC150V_10105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR3763311.1"
                     /translation="MSQPFGTLKGFGVTFRQLFRKPITQQYPEYKRAVYPRFRGRHQL
                     HRHENGLEKCVGCSLCAAACPSDCIRVVAAENTADDRVSAGERYARIYEINMSRCIFC
                     GYCELACPFDAITLGNDFELAEYSRDDLIYTKDMLLAEPIKRVPVRDADLYDTPVPAY
                     KTRS"
     gene            complement(3362..>3546)
                     /locus_tag="AC150V_10110"
     CDS             complement(3362..>3546)
                     /locus_tag="AC150V_10110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HIR3763312.1"
                     /translation="AFSGPIWVLVKLFGLLFVFIWIRTTVPRLRYDQLMRFGWKVLLP
                     VATLNAVVTAILVVAI"
BASE COUNT          683 a         1151 c         1260 g          452 t
ORIGIN      
        1 cagcgcgatc gaggcggcgg cctcgcgcgc catcggaacg agccagagca ccagcgcggc
       61 ggcaatggga aggacgatca gcagcgtggc catcagcgca ccgccatgaa cacgatcgcg
      121 aggacgacga cggacagcgc gatgacgagc gcgtacgagc ggaggtagcc ggtctgggcc
      181 ctggcgacgg ccgaagccgc ctcgcccgcc ccctcaccga tgtcggtcag ggaaccctcc
      241 acgacgggcg tctcgacgtc ggtgcgcagg cggacggcga tcatggcggc cggtcggtag
      301 aagagagcgt cgtagagctc gtctgcgtag aacttgcgct cgaagaccgc ctcgatctgg
      361 ggccacggca cgaggtcgcg tccgcgggac acggctctcc acgcgacgta gatcccgatc
      421 gctccgagcg cgacagccac ggcgctggtc gcgtagtcct cggcgactgt cggaagctcg
      481 agcggctccg cgacctgggc gacccattcc tcgaagatct cccatgcgcc cgggatgtcg
      541 aggaagccgc cgatcgtcgc cagcacggag agaacgccga ccgggatgag catcgaccac
      601 gggccctcgc gcggtccgtc accgtgagtc tccgtgacgt gctccagcgc gaactcggac
      661 ggctcgccac ggaagacgag ccagtacaga cggaacgcgt agaggccggt gagcagcgcc
      721 ccgatcaacc ccatcacgta caggaaccaa cccagggcgc ttcccgtcgc cagagccgag
      781 gcgagaatcg catccttcgt ccagaagccg gagagcggcg ggatcgcgat cagcgcgagc
      841 gccccgatca ggaacgtgat gtgggtgaac ggcatgtagc ggtgcagacc gcccatcttg
      901 cgaatgtcct gctcgcccgc gagcttgtgg atgacgacgc ccgctccgag gaagaggagc
      961 gccttgaaaa acgcgtgggt gacgaggtgg tacatggcga agccgtacgc accgacgccg
     1021 acgcccacga acatgtagcc gatctgcgac atcgtcgagt aggcgatcac gcgtttgatg
     1081 tcctgctgca cgagcgcgac aaggccggcg aagacaaggg tcacggcacc gaggatcgcg
     1141 gccagggcct ggatgtcggg ggcggcctcg aagatcgggt gagcgcgcgc gatcaggtag
     1201 acgccggcgg tcaccatcgt cgcggcgtga atcagcgcgg agacaggggt cgggccctcc
     1261 atcgcgtcgg gcagccatgt gtggagcggg atctgggccg acttcgcgac cgcgccgccc
     1321 agcaggccga gggcgacgag gttcagctgt gtctgggaga ggtcgctccc ctcggccgcc
     1381 tcgaagacgg gcccgaagtc gagcgtcccg acctgccaga tgaggacgaa gaacgcgagc
     1441 gccatcgtcg cgtcgccgat cgcgttcatc acgaacgcct tcttggcggc ggcgaccgcc
     1501 tcggtccgct ggtggtagta gccgatcagc agatacgaag ccaggccgac gaggccccag
     1561 ccgaccagca ggaagaggaa attccccgcc tcgacgagcg tcaacatcgc gaagacgaag
     1621 agcgacatgt aggcgaagta gcggcgctcc tcgtcctcgc cgtgcatgta gccgagcgag
     1681 taccagatga tgagtccgcc gacgcccgag acgaccagca tcatcacgat cgagagcggg
     1741 tcgatcagca gcgagaaccc gacctcgaag ttgccggccg ccagccacgt gaaggcggtc
     1801 gagacccccg cccgctcgtc cggagagcgc gcccacatcg agacgaaggc gagcatcgcg
     1861 ccggcgaacg cgaggaaggt cgtcccgatc gagatgaacg ctgcggtgcg ccgcgagacg
     1921 agcggaccga gcacggtgat cagcaaggcg ccggcgaggg gtgcgagcag gcagatccag
     1981 ccgccgacga tcacccggac agctccttga agcggtcgac gtcgagcggc agccggcgcc
     2041 gcgccatggc gacgatcagc ccgaggccga cggtgacctc agctgcggcg acggccatca
     2101 cgccgagcgc gaacacctgg ccgtccacgg cgccgaactg gcgcgagaac gcgaccagcg
     2161 cgaggttggc gctgttgagc atgatctcca gcgaaagcag gacgatcagc gtgctgcgcc
     2221 gcagcaggac gccggccgtg ccggtcgcga acaggatccc ggcgacggcg aggtaccagg
     2281 gaatgcccgg gccggtcacc gttcgccctc ctcgtcgcgt tcggccgagt ctctggcact
     2341 catgccgagc acgacgccgc cgattgcggc gacgagcagg atgatcgagg tgatctcgaa
     2401 ggcgagcagg tggtcggtca ggaacagctc accgatcgct gccgggctcc cgaaggcacg
     2461 ggtgatgtcg gcgggatcgg agaggctgtc gttggcccac tgggcgaacg cgacgaccac
     2521 cacgaccaga agtgccgccg ctgcgcccgc agcggcgacc tggaacaacc gtgttccccc
     2581 ggcccaggga gcgtcggctc gcccgcccag gtaggcgatc acgaagagga acatcaccat
     2641 taccgcgccg gcgtagacca gcacctgtgc ggcagcgacg aactccgcgg aagcgagcag
     2701 gaacagcacg gcgagcgagg cgaggttccc gatcagggcg agggcgctgt agaacgggtt
     2761 ggcgaaggtg atgacggcga cgccggcgcc gatgcagccg agtgcggcga ggatccagac
     2821 cagccagacg aggaaggtgc ccacccggcc tccctaagac ctcgtcttgt acgcgggcac
     2881 cggcgtgtcg tagaggtcgg cgtcgcggac gggcactcgc ttgatcggct ccgcgagcag
     2941 catgtccttc gtgtagatga ggtcgtcccg cgagtactcg gcgagctcga agtcgtttcc
     3001 gagcgtgatg gcgtcgaagg ggcaggcgag ctcgcagtag ccgcagaaga tgcagcggct
     3061 catgttgatc tcgtagatgc gcgcgtagcg ctcgcctgcc gagacacggt cgtcggcggt
     3121 gttctcggcg gccacgacgc ggatgcagtc ggaagggcag gcggccgcgc agagcgagca
     3181 gccgacgcac ttctccaggc cgttctcgtg ccggtgcagc tggtggcgac cacggaagcg
     3241 cgggtacacc gcgcgcttgt actcggggta ctgctgggtg atcggcttcc ggaacagctg
     3301 tctgaacgtg acgccgaagc ccttgagggt gccgaacggc tgactcatcg tggtgacccc
     3361 atcagatcgc caccacgagg atcgccgtga cgaccgcatt gagcgtcgcg accgggagca
     3421 gcaccttcca gccgaagcgc atcagctggt cgtagcgcag ccgcggcacg gtggtgcgga
     3481 tccagatgaa gacgaagagc aggccgaaga gcttgacgag gacccagatc gggcccgaga
     3541 acgccc
//
LOCUS       DBNGNK010000343         3727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1636532,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000343 DBNGNK010000000
VERSION     DBNGNK010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1636532"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC150V_10115"
     CDS             complement(<1..815)
                     /locus_tag="AC150V_10115"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3763313.1"
                     /translation="MRRHRTTRTGTLLALAAALLVVGPASATTPGSGGPPGEAPPTAG
                     LVDGEPIVFPLLSRVWYTDDFGDPRPNGSHEANDIKGDWRAPVLAAEAGRVKWWTTSA
                     RAGCMLYLYGKSGITYIYIHLNNDLTRKRDNRAGCKLGTAYAVKNGARVRAGQQIGFN
                     GDSGDAEGNYHLHFEVRPDDVATSPFPYLQIAHRLLVPTPTGRRFSAAFRGTPVSAGD
                     GVLELQVTSVRWWPGGRWTPIDASRIEVTVPPEADVDVALEEAVKGPARRTLAA"
     gene            932..1711
                     /locus_tag="AC150V_10120"
     CDS             932..1711
                     /locus_tag="AC150V_10120"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR3763314.1"
                     /translation="MKIAVCVKQVPDATVSKRIDPATNRLDRSGEAALNPTDVNAVEE
                     ALRVKEARGDGQVTVVSLGPSKAVESLRKALAMGADRAVLVADESAAGSDLVATSYAL
                     AKALEREQADLVLFGQQSSDADGAVLWAAVADRLRLPLISQVAELTLDGDKVTGKRQT
                     EHGYDVIAASLPAVVAVSDAINEPRYPSLKGIMGAKKKPAEELTLADLGVEAERSGEA
                     GSRTTVVALNPPPARGDTVKIEDDGSAAEKIVDYLAEKRLL"
     gene            1713..2666
                     /locus_tag="AC150V_10125"
     CDS             1713..2666
                     /locus_tag="AC150V_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR3763315.1"
                     /translation="MTDTLVFLEHHGDQLQKGGLGVLGKATSLGGEVAGVVLGEGVRD
                     VAASAGGAKVFVCDDPALGPPLPQPRVDALAALVEQTGAETILFASSVLAADVAAGLA
                     ARLDAGLNWDITDLDVSNGDLVAKRPALGDTVLVDAGWKGTPRLALVRSGTFDPPETA
                     GSSDVEEFTASFEDFSTRAVLVSQETEEASGPSIEDADVIVAGGRGLGSPEGFALCEE
                     LAQALGGAVAATRAVVDAGWYPYATQVGQTGKSVAPRLYIACGISGAIQHKVGMQSAG
                     TIVAINKDPNAPIFDFADLGVVGDLNEIVPKLAELVRARNG"
     gene            2672..>3727
                     /locus_tag="AC150V_10130"
     CDS             2672..>3727
                     /locus_tag="AC150V_10130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3763316.1"
                     /translation="MRPADFPPPFASAEAVGPPSDPPDERIEVGVLVVGGGPAGLSCA
                     IRLGQLIEEHPDVGERLGDVPVAVVDKGKQPGSHLLSGAVVNPRALRRLFKGRLQMED
                     IPNFGPVEEEAVYLLTEQKALRIPPPPTMRNHGNWIVSLAQLGSFLSEQAEELGAMVL
                     SETSAEKLLVEHGRVHGARTGDKGRGRDGAELGNFEPGSDITAQVTVLAEGTAGHLTL
                     AAIDRFGLQGQNPQIWALGVKEVWRVEKPLHNVVHTLGWPLRKRKRYREFGGSWIYPM
                     GEDMISLGFVAGLEYADVELSVHDLLQQFKTHPFMRKLLAGGQRVAWGAKTITEGGYL
                     SVPRKLNAPGLLLAGESA"
BASE COUNT          578 a         1251 c         1338 g          560 t
ORIGIN      
        1 gcggcgagcg tccgcctcgc cgggcccttg acggcctcct cgagggcaac gtccacatca
       61 gcctcgggag ggaccgtcac ctcgatgcgg ctcgcgtcga tcggcgtcca gcgacccccg
      121 ggccaccagc gtacgctcgt cacctgcagc tcgagcacgc cgtcgccggc agagaccggg
      181 gtgccgcgaa aagcggccga gaagcgcctg ccggtaggag tcgggacgag gaggcggtgg
      241 gcgatctgca ggtacgggaa ggggctcgtt gccacgtcgt cggggcggac ctcgaagtgg
      301 aggtggtagt tcccctcggc gtccccggag tcgccgttga agccgatctg ctgccccgcg
      361 cgcaccctcg cgccgttctt gaccgcgtag gccgtgccga gcttgcaccc ggctcggttg
      421 tctcgtttgc gggtcaggtc gttgttcagg tggatgtaga tataggtgat gccgctcttg
      481 ccgtagaggt agagcatgca gccggcgcgc gcggatgtcg tccaccattt cacccggcct
      541 gcctctgcgg cgagcaccgg tgcgcgccag tcgcccttga tgtcgttggc ctcgtgcgag
      601 ccgttcgggc gcgggtcgcc gaagtcgtcc gtgtaccaga ccctcgagag cagcgggaac
      661 acgatcggct ccccgtcgac cagaccggcg gtcggcggcg cctcgcccgg agggccgccg
      721 gatcccggcg tcgttgccga cgccggcccc acgacgagga gggcggcagc gagggcgaga
      781 agagtgcccg tgcgtgtcgt ccggtggcgt ctcatggctc cggtgcgcgg caacggaaga
      841 gcgtcaccct accagggcgc gaagcggcca acggcaccgt ggctctgcca accgacaccc
      901 ccgccgaggg gctgtcgtta gagtcttcgc cgtgaagatc gccgtctgtg tcaagcaagt
      961 tccggatgcc acggtctcga agcggatcga ccccgcgacc aaccggctcg atcgctcggg
     1021 cgaggctgcg ctgaatccca cggacgtgaa cgccgtcgag gaggcgctac gagtgaagga
     1081 ggctcgcggc gatggccagg taaccgttgt ctcgctgggg ccgtcgaagg cggtcgagtc
     1141 gttgcgcaag gcgctcgcga tgggcgccga ccgcgccgtt ctcgtggccg acgagagtgc
     1201 cgccggctcg gatctagtgg ccacgagcta tgcgctcgcc aaggcgctcg agcgagagca
     1261 ggccgacctc gtcctcttcg gccagcaatc gagcgacgcc gacggggctg tgctctgggc
     1321 ggcggtggcg gatcgcctgc gtctcccgct gatctctcag gtggcggagc tgaccctcga
     1381 cggcgacaag gtcaccggga agcgccagac cgagcacggc tacgacgtga tcgcggcctc
     1441 gctgcccgcg gtcgtggcgg tgtcggacgc gatcaacgag ccgcgctatc cgtcgctcaa
     1501 ggggatcatg ggggcgaaga agaagccggc agaagagctg acgctcgcag acctcggagt
     1561 cgaggccgag cgctccggcg aggcgggatc gaggacgacc gtcgtggccc tcaacccgcc
     1621 gccggcgaga ggcgacaccg tgaagatcga ggacgacggc agcgcagccg agaagatcgt
     1681 cgactacctc gccgagaagc ggctgctctg acatgaccga cacactcgtc tttctcgagc
     1741 accacggcga ccagctccag aagggcgggc tcggtgtact gggcaaagcc acgtcgctcg
     1801 gcggagaggt cgcgggcgtg gtgctcggcg agggcgtgcg cgatgtcgct gcgagcgcag
     1861 gcggcgcgaa ggtcttcgtc tgcgacgatc ctgcactcgg ccccccgctc ccacagccac
     1921 gtgtcgacgc gctcgcggca ctggtcgagc agacaggagc cgaaacgatc ctcttcgcca
     1981 gttcggtgct cgccgccgac gtcgcggccg ggctcgcggc ccggctcgac gccggattga
     2041 actgggacat caccgacctg gacgtctcga acggcgatct ggtcgcgaag cggccagcgc
     2101 tgggcgacac cgttctcgtc gacgcgggat ggaagggcac gccgcgcctc gcgctcgtcc
     2161 ggtcggggac gttcgacccg cccgaaacgg ccggtagctc ggacgtcgag gagttcaccg
     2221 cctccttcga ggacttctcg acgcgcgccg tgctcgtctc gcaggaaacc gaggaggcca
     2281 gcgggccttc gatcgaggac gccgacgtga tcgttgcggg gggtcgcggg ctcggcagcc
     2341 cggagggatt cgctctgtgc gaggagctcg ctcaggcgct cggtggagcg gtcgcggcga
     2401 cgcgggcggt cgtcgacgcc ggctggtacc cgtacgcgac gcaggtgggt cagaccggga
     2461 agtccgttgc gccgcgcctc tacatcgcct gcggcatctc gggcgccatc cagcacaagg
     2521 tcggcatgca gagcgctggc acgatcgtcg ccatcaacaa ggatccgaac gcgcccatct
     2581 tcgatttcgc ggacctaggc gtggtcggtg acctgaacga gatcgttccc aagctcgccg
     2641 agctcgtccg cgcccgtaac ggatgactga tgtgcggccg gcggacttcc cgccgccttt
     2701 tgccagcgcc gaagccgtcg gcccgccgag cgatccgccc gacgagcgca tcgaggtcgg
     2761 cgtcctcgtc gtcggcggcg ggccggccgg cctgtcctgt gcgatccgac tcggccagct
     2821 catcgaggag caccccgacg tcggcgagcg gctcggcgac gttccggtgg ccgtcgtcga
     2881 caaggggaag cagcctggat cgcatctgct ctccggcgcg gtcgtcaacc cgcgcgcgct
     2941 gcggcgcctc ttcaagggac ggctgcagat ggaggacatt ccgaacttcg gcccggtcga
     3001 agaggaggcc gtctacctgc tgaccgagca gaaggcactc cgcatcccac cgcctccgac
     3061 gatgcggaat cacggcaact ggatcgtctc gctcgcgcag ctgggctcct tcctctcgga
     3121 gcaggcagag gagctcggcg cgatggtgct gtccgagacg tcagccgaga agctgctcgt
     3181 cgagcacggg cgcgtgcacg gcgcgcgtac cggcgacaag ggtcgaggcc gcgacggggc
     3241 ggagctcggc aacttcgagc cgggctccga catcactgcg caggtcaccg ttctcgccga
     3301 gggcacggcc gggcatctca cgctcgcggc aatcgaccgc ttcggtctcc agggacagaa
     3361 cccgcagatc tgggcgctcg gcgtcaagga ggtctggagg gtcgagaagc cgctccacaa
     3421 tgtcgtccac acgctcggct ggccgctgcg caagcgcaag cgctaccgcg agttcggcgg
     3481 atcgtggatc tacccgatgg gggaggacat gatctcgctc gggttcgtcg caggcctcga
     3541 gtatgcggat gtcgagctct ccgtccacga tctgctgcag cagttcaaga cccatccctt
     3601 catgcgcaag ctgctcgccg gcggtcagcg agtcgcgtgg ggcgccaaga cgatcacgga
     3661 gggtgggtat ctgtcggtgc cgagaaagct gaacgcgccc gggctcctgc tcgcgggcga
     3721 gagcgcg
//
LOCUS       DBNGNK010000344         9256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_560499,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000344 DBNGNK010000000
VERSION     DBNGNK010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_560499"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2464)
                     /locus_tag="AC150V_10135"
     CDS             complement(<1..2464)
                     /locus_tag="AC150V_10135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR3763317.1"
                     /translation="MGLALVVGPAHAGKVALLLEQYVQALSADPWLIVPNRPDVERVE
                     RDLLHRSPVLFSGTIGTFDDLFREIAATDPARRPVATDAQRALAVRRTIGEARLNGLG
                     RSARSSGFADALETALGELESSLVEPDALPGDLAELHQGYLAELDRLGLWDRDVVRRR
                     AADRLSSDLDAWSGRPVFAYGFEDLTGAEWALLEALAGRSDVTVSLPYEPGRSAFASL
                     ARTAEDLSALAGERIIELPPRAAGAAPPALDHLERVLFVDDPPAPPPLGGAIRFLEGA
                     GARGTIELVAQQLIELLGAGTPADRIGIVCDSIERRRAPLESVLGPLEIPYGIEERIR
                     LAQTSLGRAFISLLRFAWLGGDRGDLFSFLRSPYSGLPRASVDYLEGRLRGRAVHEPA
                     RVVEEAERLRGAPLDVLAELRGERPPVDEARTVLRTFARRAYGLDEPPVGDEARADLS
                     ASETLLRLLDELDEWQSLDRQLHQDEVVAAVERATLAPALAGARGRIAVLDLMRARTR
                     DFDAVFVLGLEEGSLPRRGRPSPFLDDERRHALGGRLERPDQVSRDRYLFYTACTRAE
                     RRLYLVREATDDDGRAREPGPFWDEVTRLFTADDVRHATTRRSLSALTWTLEGAPSER
                     ERLRALARVAVDEPDDAYALAAANGWTRRLERATRAFSRPTRLQNPAVTGVLQARTSF
                     SVTELERFADCSSAWLVERVVQPKTIDAEPDPLLRGNIAHTTLSRFYGGLPKELGVER
                     VTTELAERALEFVRRCLDEALASGVRIDLTDLQRAELRQSLRRDLEAFVRAEAESELA
                     LVPRRFEVGFGSDRAAPELQRGL"
     gene            complement(2573..2770)
                     /locus_tag="AC150V_10140"
     CDS             complement(2573..2770)
                     /locus_tag="AC150V_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763318.1"
                     /translation="MARKTVLVCDACGNEVAEGKGAVMRVTYTDARRGAKQADLCDGC
                     AGGTPGVAVARRGRKPKSVTG"
     gene            2910..3983
                     /locus_tag="AC150V_10145"
     CDS             2910..3983
                     /locus_tag="AC150V_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3763319.1"
                     /translation="MDSRTIVVLEGDQTGQELLEEALRVLAADVIGVALSLPRFDLSL
                     ENRRATENAVVYEAAQAIREHGLGLKAATITPEQPGDVGSPNRILREEIDGKVIVRTG
                     RRIPGVVPFGGVHAPISVVRMAVGDAYGASEWREGEGENEVAFRTERIERRICHAVAE
                     FAFRQAERTGASVFGGPKYTVSPVYEGMLKEEMDAAAERHPDVPYEPQLIDATFALLI
                     SSSGSPLVIPALNRDGDILSDLVLPLFGSIAGSESLVVSFDDDYVPDAVMAEAAHGTA
                     PSLEGKDVANPMAMILAAAALLTHADEKACQTAGRAIRESALEAVSEGVKTPDLAGHA
                     STGEFTDDVIRRTRTKIEIWATL"
     gene            complement(4010..4588)
                     /locus_tag="AC150V_10150"
     CDS             complement(4010..4588)
                     /locus_tag="AC150V_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR3763320.1"
                     /translation="MTLATFSAVLQACDPELGLHAARVSVNAQAVARRLGWDDSQLGD
                     VAIGAALHDVGKMNTRPEILAKPGPLDDEEVAHVRAHPVEGAWLVAGVSSLRAALPFV
                     LFHHERWDGGGYPTGRARTEIPVEGRLLAVVDAFDAMTSPRPYREAMEPGEALFELDR
                     CAGSQFDPVITGAFIDAWHAGEVSPMQLAATA"
     gene            4643..5758
                     /locus_tag="AC150V_10155"
     CDS             4643..5758
                     /locus_tag="AC150V_10155"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR3763321.1"
                     /translation="MRVAVPRETAPGEHRVALIPETAGRLAGAGFDIVVEQGAGEAAG
                     FADSAYAGAGAEVSAPSALLEGAAGIVRVARPNSDEVAALAAGTVVVGFLAPLTDPEG
                     IERLRAQGAIAFAMESIPRITRAQPMDALSSQATVAGYKAVLLAADRMQKLFPLMMTA
                     AGTIPPARVLVLGAGVAGLQAIATARRLGALVSAFDVRPAVKEQVESLGATFLELGVR
                     AEETEGGYATELTAEEQAQQQAELEQHIADMDAVITTALVPGRPAPKLIPGAAVAAMR
                     DGSVIVDLAAEAGGNCELTEPGEAVRREGVTIVGFTNLPSMLASDASRLYSRNIAALL
                     GHLAPGGELELDFEDEITRGACVTGTPPGTGAGEEQT"
     gene            5755..6057
                     /locus_tag="AC150V_10160"
     CDS             5755..6057
                     /locus_tag="AC150V_10160"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR3763322.1"
                     /translation="MSTSLVLSVTIFALAAFLGFELIARVPTLLHTPLMSGTNAISGV
                     VIVGAILVLGSSDETLELVLAFVALVFATINVVGGFLVTDRMLEMFKRRPEDPPDS"
     gene            6054..7457
                     /locus_tag="AC150V_10165"
     CDS             6054..7457
                     /locus_tag="AC150V_10165"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017714837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR3763323.1"
                     /translation="MIAFSFSESVVDLAYVVAAGCFIIGIRMLSSPRTARLGNVVAAV
                     GMLVAVAFTLTLKEVDRYWLVALGIAAGVVIGIPAARLVRLTAMPQMVALLNGFGGGA
                     SAAVALAEFNRVATTPGSSDAQIVVASVFSALIGSLTFSGSLVAFSKLQELMTGRPIV
                     WPAQQYLNAGLGVAALGLGIAAGVTESRGLIVGMLVCALALGVLIVLPIGGADMPVVI
                     ALLNAASGLSAVGAGFVLSNNALIIGGALVGASGTLLSILMGRAMNRSMANVLFGAFG
                     QVQAGAGAAVVDDGRTVRATTPDDVAVMLAYARRVIVVPGYGLAVAQAQHDVRQLADL
                     LEEKGVDVRYAIHPVAGRMPGHMNVLLAEANVPYTQLYEMDSINSEFPQTDVALVIGA
                     NDVTNPAAREETSSPIYGMPILDVDKAASIVVLKRSMNPGFAGIDNALYLDPKTTMLF
                     GDAKESVAKLVTAVKTV"
     gene            complement(7470..7622)
                     /locus_tag="AC150V_10170"
     CDS             complement(7470..7622)
                     /locus_tag="AC150V_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763324.1"
                     /translation="MPLTYAYNALERVTAEAPLGARFWVDVTVVAIVTVLALALGAAT
                     LRRRTA"
     gene            complement(7644..7808)
                     /locus_tag="AC150V_10175"
     CDS             complement(7644..7808)
                     /locus_tag="AC150V_10175"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763325.1"
                     /translation="MALAIGNAVLGMSLGFFVSAFARSEFQAVQFMPAFVFPQLLLCG
                     LLVPREQMAG"
     gene            complement(7847..8056)
                     /locus_tag="AC150V_10180"
                     /pseudo
     CDS             complement(7847..8056)
                     /locus_tag="AC150V_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            8105..9040
                     /locus_tag="AC150V_10185"
     CDS             8105..9040
                     /locus_tag="AC150V_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3763326.1"
                     /translation="MIVPVFRAGDVERAVPPHEAYDAVREAFLAHARGEWTMQPKVYV
                     ANYPAGDLRAMPALGDGHALLKWIGSYPGNPARGLPTVAGVVVLSDAETGLVLALLDA
                     AAVTALRTAAAGVLAADVLGRPGAASLSVIGCGVNGAETVRMLRAQGRDVLIHDADAR
                     RQTAVAEALGATGVGRDDAVRADVVITVTPGREVVFPAGSLRDGQHVSLIGADGPGKA
                     EIAVEELARGHVFCDDWEQASHGGELTAAVAAGVLSRERVTELGAVLLRRAEGRRSAR
                     DITLFDSTGLAVQDLAIAKAAYAKADELDLQRIEL"
     gene            complement(9045..>9256)
                     /locus_tag="AC150V_10190"
     CDS             complement(9045..>9256)
                     /locus_tag="AC150V_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3763327.1"
                     /translation="RWAGIFGMVFDFLPVVGRVPGQPGLWVAGGYSGHGNVLGYACGA
                     LAARAISGVSDPLLALFEPARLLAQ"
BASE COUNT         1451 a         3198 c         3244 g         1363 t
ORIGIN      
        1 ggaggcctcg ctgcagctcc ggcgcggcgc ggtccgagcc gaagccgacc tcgaaacgcc
       61 tcggcacgag cgccagctcc gactcggcct cggcgcgcac gaaggcttcg aggtcacgcc
      121 gaagcgattg gcgcagctcg gcccgctgga gatcggtgag atcgatccgc acgccgctgg
      181 cgagggcttc atcgaggcag cgccggacga actcgagcgc gcgctcggcc agctccgttg
      241 tgacgcgctc gaccccgagc tctttcggca gtccgccgta gaagcgcgac agtgtcgtgt
      301 gggcgatgtt tccgcggaga agcggatcgg gctcggcgtc gatcgtcttc ggctggacga
      361 cacgctcgac gagccacgca gaggagcaat ccgcaaatcg ctcgagctca gtcaccgaga
      421 agctcgtgcg agcctgcaac acgcccgtca cggcggggtt ctggagccgg gtcggacgcg
      481 agaacgctcg agtcgcgcgt tccagtcgcc tcgtccagcc gttcgccgcg gcgagcgcat
      541 acgcatcgtc cggctcgtcg accgcgacgc gtgccagcgc acgcaggcgt tcgcgctcgc
      601 tgggcgcgcc ctcgagcgtc cacgtgagtg ccgacagcga ccggcgagtc gtcgcatgcc
      661 gcacgtcgtc ggccgtgaac agtcgggtca cctcgtccca gaacggcccc ggctcgcgcg
      721 ccctaccgtc gtcgtcggtc gcctcgcgga cgagatacag gcggcgctcc gcgcgcgtgc
      781 acgccgtgta gaagaggtac cggtcgcgac tcacctgatc gggacgctcg aggcgcccac
      841 ccagggcgtg ccgccgctcg tcgtcgagga aaggtgacgg gcgtccccgc ctgggcaggc
      901 tcccctcttc gaggccgagc acgaagacgg cgtcgaagtc gcgggtgcgc gcccgcatca
      961 ggtcgagaac ggcgatgcgc ccacgggcgc cggcgagagc cggtgccagc gttgcgcgct
     1021 cgacggccgc gacgacctcg tcctggtgca gctgacggtc gagcgactgc cactcgtcga
     1081 gctcgtcgag caggcgcaac agggtctcgg acgccgacag gtcggcgcga gcctcgtcgc
     1141 ccacgggcgg ctcgtcgaga ccgtaggcac gcctcgcgaa tgtgcgaagc accgtccgcg
     1201 cttcatcgac gggagggcgc tctcctcgga gctcggcgag gacgtccagc ggggcgccgc
     1261 gaagccgctc ggcctcctcc accacccgcg ccggctcgtg caccgcccgg ccgcgcagcc
     1321 gaccttcgag gtagtcgacg gatgcgcgcg gcaggcccga atacggcgag cgcaagaagg
     1381 agaacagatc gccacggtcg ccgccgagcc acgcgaaacg gagaagtgag atgaacgctc
     1441 tcccgagcga cgtctgggcg aggcggattc gctcctcgat tccgtaggga atctcgagcg
     1501 ggccgagcac cgactcgagc ggcgcccgcc ggcgctcgat cgagtcgcac acgattccga
     1561 tccggtccgc gggtgtccct gcaccgagca gctcgatgag ctgctgtgca acgagctcga
     1621 tcgtgccgcg ggcgcccgca ccctcgagga agcgaatcgc gcccccgagc ggcggcggcg
     1681 ccggcgggtc gtcgacgaag agcacgcgtt cgagatggtc gagtgccggt ggcgccgccc
     1741 cggcggcacg cggcggaagc tcgatgatcc tctcgccggc gagcgcggag aggtcttccg
     1801 ccgtccgcgc gagcgaggca aacgccgacc gtcccggctc gtacggcagc gagacggtga
     1861 catcggaacg gcccgccagc gcttcgagca gagcccactc ggcgccggtc aggtcttcga
     1921 aaccgtaggc gaagaccggg cgaccactcc aggcgtcgag atcgctggac agtcgatccg
     1981 cggctcgccg ccgcacgaca tcgcggtccc agagcccgag gcgatccagc tcggcaaggt
     2041 atccctggtg gagctcggcg aggtcgccgg ggagggcatc cggctcgacg aggctggact
     2101 cgagttctcc gagcgctgtc tccagcgcgt ccgcgaatcc gctgctgcgc gcggatcgac
     2161 cgagaccgtt gagacgcgcc tccccgatcg tccggcggac ggccagcgca cgttgcgcat
     2221 cggtggcgac aggacgccgc gccggatccg tcgcagcgat ctcccgaaag aggtcgtcga
     2281 acgtgccaat cgtgccggaa aacagaacgg gacttctgtg cagcaggtca cgctcgaccc
     2341 gctcgacgtc cggtcgattc ggcacgatca accagggatc ggccgaaagc gcctgcacgt
     2401 actgctcgag aagcagcgcg actttgccgg catgcgccgg cccgacgacg agcgcaaggc
     2461 ccattcgttc atcgtaatcc cgggctccga cggctcctct tacgccgcac gcaaagagcg
     2521 ggatttactc gcgcccgagc gggaggggcc tgcgagaagg ggctctgcgc tgctagccgg
     2581 tgaccgactt cggctttcgt ccccgccgcg cgaccgcgac gccgggcgta ccgcccgcac
     2641 agccatcaca gagatcggcc tgcttggctc cacgtctggc atccgtatag gtgacgcgca
     2701 tgaccgcgcc cttcccttcg gccacttcgt tgccgcacgc gtcgcacacg agcactgtct
     2761 ttctagccac tgtttgcctc ccgcgattcg atctagatcg gaaacatacc ctaatcaatt
     2821 ccggcgaatt tacgcaagcc tataacagtc cacaattgtc gctatttcaa cgaaggccgg
     2881 attgcgcgac gctgactaga atcgcccgaa tggactcgcg cacgatcgtc gtgctggaag
     2941 gcgaccaaac gggacaagag ttgctggagg aggcgctccg ggtgctggcg gccgacgtca
     3001 tcggcgtggc gctcagcctt ccccgtttcg acctctcact cgagaaccgc cgtgcgaccg
     3061 agaacgccgt cgtctacgaa gcggctcagg cgatccgcga gcacggactc ggtctgaagg
     3121 ccgcgacgat tacgccggag cagcccggcg acgtcggctc gcccaaccgg atcctgcgcg
     3181 aggagatcga cggcaaggtg atcgttcgga caggccgcag gattccgggc gtcgtaccct
     3241 tcggcggggt gcacgcgccg atctcggtcg tgcggatggc ggtcggcgac gcctacgggg
     3301 cgtccgagtg gcgcgagggc gaaggagaga acgaggttgc cttccgcacc gagcggatcg
     3361 agcgccgcat ctgtcacgcc gtggcggaat tcgcgttccg gcaggcggag cgcaccggcg
     3421 cgtccgtctt cggtggcccc aaatacaccg tcagccccgt ctacgaaggg atgctcaagg
     3481 aggagatgga tgcggcggcg gaacgccacc ccgacgtccc gtatgagccg cagctgatcg
     3541 acgcgacctt cgcgctcctg atctcgtcga gcggctcacc cctcgtgatt ccggcgctca
     3601 accgtgacgg agacatcctc tccgacctcg tcttgccgct gttcggctcg atcgcgggct
     3661 cggagtcgct cgtggtgtcc ttcgacgacg actacgtgcc cgacgcggtc atggccgagg
     3721 cggcacacgg gactgcgccc tcgctcgagg gcaaggacgt agcgaatccg atggcgatga
     3781 tccttgccgc cgcggcgctg ctcacacatg ccgacgagaa ggcatgccaa acggccgggc
     3841 gggcgattcg cgagtcggcc ctcgaagcgg tgtcggaagg cgtgaagacg ccggacctcg
     3901 ccggccacgc gagcaccggc gagttcacgg acgacgtgat ccgccgcacg cggacaaaga
     3961 tcgagatctg ggccacgctc tgagcccggg aaggacgcgc cgtgggctcc tacgcggtgg
     4021 ctgcgagctg catcggcgac acctcgccgg cgtgccacgc gtcgatgaag gcgccggtga
     4081 tcactggatc gaactggctg ccggcgcagc ggtcgagctc gaaaagcgcc tcgcccggtt
     4141 ccatggcctc gcgataggga cggggcgacg tcatcgcgtc gaatgcgtcc acgaccgcca
     4201 gcaaccgtcc ttcgaccggg atctcagttc tggcacggcc ggtcggatag ccgcctccgt
     4261 cccagcgctc gtgatggaag agcacgaacg ggagcgcggc gcgcagtgag ctgacaccgg
     4321 ccacgagcca cgcgccttcg acggggtgag cccgcacatg agccacctcc tcgtcgtcga
     4381 gcggaccggg cttggcaagg atctccggac gggtgttcat cttcccgacg tcgtggagtg
     4441 cggcgccgat cgcgacgtcg ccgagttgcg agtcgtccca gccgagccgc cgcgcgaccg
     4501 cttgggcgtt gaccgagacg cgggcggcgt gcagccccag ctctgggtcg cacgcctgca
     4561 gcacggccga gaaggtcgca agagtcatgc tgagtgacgt tcccctctca cgcgccgatc
     4621 ccttctcgta gagtcgtctc gtatgagagt ggctgtgcct cgggagactg cgcccggcga
     4681 gcatcgtgtt gctctgatcc ccgagacggc aggtcggctg gcgggggccg gcttcgacat
     4741 cgtcgtcgag cagggggcgg gcgaagctgc cggcttcgcc gactcggcct acgcgggcgc
     4801 gggcgcggag gtgagcgcgc cgtcggcgct gctcgagggc gccgcgggaa tcgtgcgggt
     4861 ggcccgaccg aacagcgacg aggtcgccgc ccttgccgcg ggcacggtcg tcgtcggctt
     4921 cctggcgccg ctcaccgatc cggagggcat cgagcgcctc cgcgcgcagg gcgcgatcgc
     4981 cttcgcgatg gagtccattc cgcgcatcac gcgcgctcag ccgatggatg cgctctcctc
     5041 gcaggcaacc gtggcgggat acaaggcagt ccttctggca gccgaccgca tgcagaagct
     5101 gtttccactc atgatgaccg ccgctggaac cattccgccc gcgcgtgtac tggtactcgg
     5161 ggcgggcgtt gcgggcctgc aggcgatagc cacggcccgc cgcctcgggg cgctggtctc
     5221 ggccttcgac gtccggccgg ccgtgaagga gcaggtcgag tctctcggcg cgaccttcct
     5281 cgagctgggg gtgcgggccg aagagacgga gggcggctac gccaccgagc tgacggccga
     5341 ggagcaggca cagcagcagg ccgagctcga gcagcacatt gccgacatgg acgcagtcat
     5401 caccacggca ctcgtgcccg ggaggcccgc ccccaagctg attcccggag ctgccgtcgc
     5461 ggcgatgcgg gacggctccg tgatcgtcga cctggcggca gaagccggcg gcaactgtga
     5521 gctgactgag ccgggcgagg cagtccgtcg cgaaggcgtc acgatcgtgg gcttcacgaa
     5581 cctcccctcc atgctggcca gcgacgcgag ccgtctctat tcgcggaaca tcgcagccct
     5641 gctcgggcac ctcgctcccg gcggcgagct cgagctcgac ttcgaggacg agatcacgag
     5701 gggcgcgtgc gtgacgggga cacctcccgg aacgggcgcc ggcgaggagc agacgtgagc
     5761 acgagcttgg tgctctcggt gaccatcttc gcgctggccg cgttcctcgg cttcgagctg
     5821 atcgcccgcg tgccgacgct gctgcataca ccgctgatgt cgggcacaaa cgccatctcg
     5881 ggcgtcgtga tcgtgggcgc aatcctcgtc ctcgggtcgt ccgacgagac gctggagctc
     5941 gtgctggcct ttgtcgccct ggtcttcgcg acgatcaacg tcgtcggggg cttcctcgtc
     6001 accgaccgga tgctcgagat gttcaagcgc cgtccagagg atcccccgga ctcgtgatcg
     6061 ccttctcctt ctccgagagc gtcgtcgacc tcgcctacgt cgtggccgcg ggctgcttca
     6121 tcatcggcat ccgcatgctg tcgtcaccgc gcaccgcgcg gctcggcaac gtggtcgccg
     6181 ccgtcggcat gctggtggcc gtcgccttca ccctgacgct gaaggaggtc gatcgctact
     6241 ggctggtcgc gctcgggatc gccgccggcg tggtgatcgg cattccggcc gcgcgcctcg
     6301 tccggctcac ggcgatgccg cagatggtcg cgctcctgaa cggcttcgga ggcggcgcct
     6361 cggcggccgt ggcgctggcc gagttcaacc gggtcgccac gacccccggc tcgtcggatg
     6421 cccagatcgt ggtcgcgtcg gtgttctcgg ccctcatcgg ctcgctcacc ttctcgggca
     6481 gcctcgtcgc gttctcgaag ctgcaggagc tgatgacggg acggccgatc gtgtggcccg
     6541 cgcagcagta cctgaacgcg gggctgggag tagcagccct cggactcgga atcgccgcgg
     6601 gggtgacgga gtctcgtggc ctgatcgtcg ggatgctcgt ctgcgcgctg gcgctgggcg
     6661 tcctgatcgt gctgccgatc ggcggcgcgg acatgcccgt cgtcatcgcc ctgctcaatg
     6721 ccgcgtcggg cctgagcgcg gtcggcgcag gcttcgtcct cagcaacaat gctctgatca
     6781 tcgggggtgc gctcgtcggc gcctcgggga cgctcctctc gatcctcatg gggcgcgcga
     6841 tgaaccgatc gatggccaac gtcctcttcg gtgcgttcgg ccaggtgcaa gcgggcgccg
     6901 gtgccgcggt cgtggacgac ggccgcacgg ttcgtgcgac gacgcccgac gacgtcgcgg
     6961 tcatgctcgc ctacgcgagg cgggtgatcg tcgtcccggg atacggcctc gctgtcgcgc
     7021 aggcgcaaca cgacgtccgc cagctcgccg accttctgga ggagaagggc gtcgacgtcc
     7081 gctacgcgat ccatcccgtc gcgggccgca tgccgggcca tatgaacgtc ctgctcgccg
     7141 aggcgaacgt cccctacacg cagctctacg agatggacag catcaactcg gaattcccac
     7201 agacggacgt cgcgctggtg atcggggcga acgacgtcac gaacccggca gcgcgggagg
     7261 agacgtccag cccgatctac gggatgccga ttctcgacgt cgacaaggcg gcgagcattg
     7321 tggtgctcaa gcggtcgatg aatccgggct tcgcgggcat cgacaatgcg ctgtatctag
     7381 acccgaagac gacgatgctc ttcggcgatg ccaaggagtc ggtcgccaag ctcgtcacgg
     7441 cggtcaagac cgtctagcgg aaacgccggt catgccgtgc ggcgtcgcag cgtcgccgca
     7501 ccgagggcta gtgcgagcac ggtgactatg gcgacgacgg tgacgtcgac ccagaaccgc
     7561 gcgccgagcg gcgcctcggc ggtcacgcgc tcgagggcgt tgtaggcata cgtcagcggc
     7621 atcgcggccg agatcgcctc gagctacccg gccatctgct cacgcggaac cagcagcccg
     7681 cacagcaaca gctgcgggaa gacgaaggcc ggcatgaact gcaccgcctg gaactcgctc
     7741 cgcgcaaacg cgctgacgaa gaacccgagc gacatcccga gcaccgcgtt cccgattgcg
     7801 agcgccacct ctcctacagg gcgcgccacc tgaaggtcga gtgcgccgag cccgcctggc
     7861 gaccgagaac ggtcacggcg ccggacgtgt caacctgtac gccgacgacg gcccgcatga
     7921 gcgtcgtctt gcggctgccg ctggggccga gcagcccagt caccagaccc ggctcgacct
     7981 cgagcgagac gccccgcagc gcctcggtgc gcccgcggac gacgcgcagg tcacggacga
     8041 cgaccgaagc agccacgcga cgattccacc acagctcacg atgggcgtgt cgtaggctgc
     8101 gggcgtgatc gtcccggtct ttcgtgcggg tgacgtcgag cgggcggtgc cgccgcacga
     8161 ggcctacgac gccgtgcgcg aggcgttcct cgcccacgca cgcggcgagt ggacgatgca
     8221 acccaaggtg tacgtcgcca actatcccgc gggcgacctg cgcgcgatgc ccgcacttgg
     8281 cgacgggcac gcgctgctca agtggatcgg ctcctatcct ggcaatccgg cgcggggcct
     8341 cccgaccgtg gcgggggtcg tggtgctttc cgacgccgag accgggctcg tgctcgcgtt
     8401 gctcgacgct gcggccgtga cggcactgcg gacggcagcg gcaggagtgc tcgcggctga
     8461 cgtgctgggc cgcccgggcg cggcgtcgct gtcagtgatc ggctgcgggg tgaacggcgc
     8521 cgagacggtt cggatgctgc gcgcgcaggg ccgcgacgtg ctgatccacg atgcagacgc
     8581 ccgccgccag accgccgtgg cggaggcgct gggagcgacc ggagtcggcc gcgacgacgc
     8641 agtccgcgcc gacgtggtca tcaccgtgac gccgggccgc gaagtcgtgt tcccggcggg
     8701 ctccctgcgg gacggtcaac atgtatctct gatcggagcc gacggccccg gcaaggcgga
     8761 gatcgcggtc gaggagcttg ctcgggggca cgtcttctgc gacgactggg agcaggcgag
     8821 ccacggcggt gagctgaccg ccgcagtagc ggccggcgtg ctgagtcgtg agcgggtcac
     8881 cgagctcggc gcggtgctcc tgcggcgcgc cgagggacgc cgcagcgccc gcgacatcac
     8941 gctcttcgat tccacggggc tggcggttca ggatctcgcg atcgcgaagg cggcctacgc
     9001 gaaggccgac gagttggatc tgcagcgaat cgagctctga gcgctcactg tgcgagcaga
     9061 cgcgccggct cgaagagcgc cagcagcggg tcggagacgc cgctgatcgc acgcgccgcg
     9121 agagctccgc acgcgtagcc cagcacgttg ccgtgacccg aatagccgcc ggcgacccag
     9181 aggccgggct gaccggggac gcgaccgaca acgggcagga agtcgaagac catgccgaag
     9241 attcccgccc agcgac
//
LOCUS       DBNGNK010000345         3210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1063117,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000345 DBNGNK010000000
VERSION     DBNGNK010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1063117"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="AC150V_10195"
     CDS             complement(<1..997)
                     /locus_tag="AC150V_10195"
                     /inference="COORDINATES: protein motif:HMM:NF015376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HIR3763328.1"
                     /translation="MATAGHRRLTSLGAFTLCLLAIAVLASAAGAGTRGTQSRSIGLP
                     WEGRLVGGVQLEQEGQYFYTWDGVQERSPNRPHRRYANERLISTLTSVLREHAAAFPN
                     APRVGIGDISRPRGGDFGVRFGGAGHASHQNGLDVDVYYPRRDKLERPPKKPKQIDRR
                     LAQDLVDRFVAAGAAKVFVGPNTGLSGPPAIVEELPAYHDNHMHVRLPGDGVSPALLG
                     ESTRGQPIRAFRLGAGPVKALVIGCIHGDECAGSVVATRLLHATPPDGGSIWIVQDLN
                     PDGHRAKRRTNARGVDLNRNFPGTWQSLATSGRTPASEVETKIAMRLIRRLRPEVT"
     gene            1114..1737
                     /locus_tag="AC150V_10200"
     CDS             1114..1737
                     /locus_tag="AC150V_10200"
                     /inference="COORDINATES: protein motif:HMM:NF021511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR3763329.1"
                     /translation="MSRRIGLPLVVVLKAMVIGLTALGAFGGLERFEEKGFGWRLVWY
                     PIAVLALPVAWRLFFRSRAYPYAADALLTLPFLIDVLGNVVNLYDTIAWWDDVNHFAN
                     WLFLSLGAGALLLRTQLGPLVIAGLVVGFGATLAIVWEIGEYATFLRTNEEELPTAYT
                     DTVADMGFGLLGSVLAALVIVLVARHRARAARDETQPTTNLALGSRA"
     gene            1763..2311
                     /locus_tag="AC150V_10205"
     CDS             1763..2311
                     /locus_tag="AC150V_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HIR3763330.1"
                     /translation="MAATRKIVVHLEETLSEGGLDVDPPTRKAVAAAVVTNPLAGRHE
                     PDLAVLEELGAAVSGRLATRAVEALSAEPEDVTAYGKAAIVGTDGELEHAAAILHPRF
                     GAPVRAAVGHGDDIIPSTKKVGGPGASITMPITNKDDIWSFDEMDAAELSIPDAPRPD
                     EVVICVAIAVGGRPHARVKKPG"
     gene            2311..2619
                     /locus_tag="AC150V_10210"
     CDS             2311..2619
                     /locus_tag="AC150V_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HIR3763331.1"
                     /translation="MFKAVILLTRAEGTTRDEFRNWWLGSHAELARGLPGLRRLVFNL
                     VETEGVPYDGVSELWFDSQEAFVAAYDSEHGKRVAADSLANVSRRERLFVDEHEIESG
                     "
     gene            2660..>3210
                     /locus_tag="AC150V_10215"
     CDS             2660..>3210
                     /locus_tag="AC150V_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763332.1"
                     /translation="MQNDGRESVPFSLLTADGERIELSYSSPHRAATHDEEVVRSELP
                     WFESGKPLRNWMLHETDFYDEVEQIWGRRWGAEGIGRLREVVVSRPTENETRPEYADQ
                     WQYYYSSRSGNADLGRLQAQFDEYYDVLERNGVRVHRIEPPVPAIGPYGYLKNLVTLA
                     GGGLVLKGGAIIHRMGLGSWQRGR"
BASE COUNT          507 a         1088 c         1106 g          509 t
ORIGIN      
        1 gcgtgacctc gggccggagg cggcgaatga ggcgcatcgc gatcttggtc tcgacctccg
       61 aggcaggcgt tctcccggag gtggcgagcg actgccaggt gccagggaag ttccggttca
      121 ggtcgacacc gcgcgcgttc gtccgtcgct ttgcccggtg gccgtcgggg ttcaggtctt
      181 gcacgatcca aatcgagccg ccgtctggcg gcgtagcgtg cagtagccgg gtggcgacga
      241 cgctgcccgc acattcgtcg ccgtggatgc agccgatgac gagcgccttc acgggtcccg
      301 cgcccagccg gaacgctcga atcggctgcc cgcgcgtgct ctcgccgagg agcgccgggc
      361 tgacgccgtc accggggaga cgcacgtgca tgtggttgtc gtggtacgcc gggagctcct
      421 ccacgatggc cggcgggccg ctgaggcctg tattcgggcc cacgaagacc ttggccgcgc
      481 cggcggccac gaagcggtcg acaagatcct gcgcgagccg acggtcgatc tgcttcggtt
      541 tcttcggcgg gcgttccagc ttgtcgcggc gcgggtagta gacgtcgacg tcgagcccgt
      601 tctggtgcga ggcgtggccg gcgccgccga aacgaacacc gaaatcgccg ccgcgcggtc
      661 gcgagatgtc gccgatgccg acacgcggcg cgttcggaaa ggcagccgca tgttcgcgca
      721 gaacgcttgt cagggtgctg atcaggcgct cgttcgcgta gcggcggtgc gggcggttgg
      781 gcgagcgctc ctgcacgccg tcccaggtgt agaagtactg cccttcctgc tccagttgga
      841 cgccgcccac gaggcggccc tcccagggga ggccgatcga gcgtgactgc gtgccgcgtg
      901 tccccgcgcc cgcagccgac gcgagcacgg cgatcgccag gagacagagc gtgaacgctc
      961 caagcgacgt aaggcggcgg tgacccgctg ttgccacccc gaaagcctac gcagggccgc
     1021 gaaccggccc gtcctgccac ctctcgcgac tactgacaca agatcagcgg gcgccccgat
     1081 gcgcgatcgg ccgcccagag ctacatgtgg actatgagta ggcgtattgg gcttccgctt
     1141 gtggtcgtcc tcaaggcgat ggtgatcggc ctgactgcgc tcggcgcatt cggcgggctg
     1201 gagcgcttcg aggagaaagg cttcggctgg cggctcgtct ggtacccgat cgccgttctc
     1261 gccctccctg tcgcctggcg actgttcttc cgcagccgag cctaccccta cgccgccgac
     1321 gcgttgctca ctctgccgtt cctgatcgat gttctcggca acgtcgtcaa cctctacgac
     1381 acgatcgcgt ggtgggacga cgtcaaccac ttcgcgaact ggctgttcct ctcactcggc
     1441 gccggcgcac tgctcttgcg gacgcagcta gggccgcttg tgatcgctgg gctcgtcgtc
     1501 gggttcgggg cgacgctcgc gatcgtgtgg gagatcggcg agtacgcgac gttccttcgc
     1561 acgaacgagg aggagctccc gacggcatac acggacacag tggccgacat gggcttcgga
     1621 ctcctcggct cggtcctcgc cgccctggtg atcgtgctcg tcgcccgcca ccgcgcccgt
     1681 gccgcaagag acgagacgca gccgacgacc aaccttgcac taggctctcg cgcataagcg
     1741 aactgcattg aaaggggagg gcgtggccgc gacccgcaag atcgtcgtgc acctcgaaga
     1801 gactttgagc gaaggcggac tggacgtcga tccacccact cggaaggcgg tcgccgctgc
     1861 ggtcgtcacg aatccgctcg caggccgcca cgagcctgac ctggccgttc tcgaggagct
     1921 cggcgcggct gtcagcggcc ggctcgcgac gcgcgccgtc gaggcgctca gcgcggagcc
     1981 cgaggacgtg accgcatacg ggaaggccgc gatcgtgggc acggacggcg agctcgagca
     2041 cgccgctgcg attctccacc ctcgcttcgg cgcacccgtc cgcgccgcgg tcggccacgg
     2101 cgacgacatc atcccctcga cgaagaaggt cggcggcccg ggtgcgtcga tcacaatgcc
     2161 gatcacgaac aaggacgaca tctggagctt cgacgagatg gacgccgccg agctctctat
     2221 cccagatgcg ccgcggcccg acgaggtcgt gatctgcgtc gcgatcgcgg tcggcggccg
     2281 cccgcacgca cgagtgaaga agccaggctg atgttcaagg cagtcatcct gctcacccgc
     2341 gccgaaggca cgacgcgcga cgagttccga aactggtggc tcgggtcgca cgccgagctc
     2401 gcacggggcc ttcccggtct ccgccgcctc gtcttcaacc tcgtcgagac ggagggcgtc
     2461 ccctacgacg gggtctccga gctctggttc gactcgcaag aggcgttcgt ggctgcctac
     2521 gacagcgagc acggcaagcg ggttgccgcc gactcgctcg cgaacgtgag ccgccgcgag
     2581 cggctgttcg tggacgagca cgagatcgaa tccggctgag cacttgcgta gcggcacggc
     2641 gccgtagcat gctcggacta tgcagaacga cggtcgcgaa tcggttccct tcagcctcct
     2701 cacggcggac ggcgagagga tcgagctctc ctactcgtca ccacatcggg ccgccacgca
     2761 cgacgaggag gtcgtgcgga gcgagctccc gtggttcgag tcaggcaagc cgctccgcaa
     2821 ctggatgctg cacgagaccg acttctacga cgaagtcgag cagatctggg gtcggcgctg
     2881 gggagcggag gggatcggcc ggctccgcga ggtcgtcgtc tcgcgtccga ccgagaacga
     2941 gacgcgtccg gagtacgccg accagtggca gtactactac tcgtcacggt cagggaacgc
     3001 cgacctcggc cgactgcaag cccagttcga cgagtactac gacgtgctcg agcggaacgg
     3061 cgtccgcgtg caccggatcg agccgccggt gccggcgatc gggccgtacg gctatctgaa
     3121 gaacctcgtc accctcgcag gcggcggact ggtgctcaag ggcggggcca tcatccaccg
     3181 catgggtctc ggctcctggc agcgtggccg
//
LOCUS       DBNGNK010000346        10709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_702977,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000346 DBNGNK010000000
VERSION     DBNGNK010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10709
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_702977"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..2322)
                     /locus_tag="AC150V_10220"
     CDS             complement(112..2322)
                     /locus_tag="AC150V_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3763333.1"
                     /translation="MRDLTGRLARASAAHPKRTLAAWGVAIVVSLAVIATLLGSSLTT
                     EGEMTNDPESYRAYDLIGQHFPFDPADVVNEVVIVRSADVKVDDPMFERAVVALAGEL
                     ESSGYAWQARTFYGTDDPSLVSPDRDAALIAVALTGEGEDKIGDVIATVDAAEKDGFE
                     TAITGEFTADHDYTELSEEDLRKGEFQFGLPAALIVLILVFGSLVAGLVPVLVALVSI
                     VVALALTALLGQAFELSIFVVNMLTGMGLALGIDYALFIVSRFREERNRGRDKLDAIA
                     VCGSTASRAVLFSGTAFVLAMVGMVLVPDTILRSLAAGAILVGIVSVVVALTLLPAVL
                     SLLGDRVNALRIPWLGRRIERSAGTEGALWSRAVRAVMRVPWVSAGLSVAVLLAVAIP
                     VLDFETGFSGIRTLPDRFAAKQGFVALEEEFGVATVDEVQVVVEGDVASPEISAGLAE
                     IAEQVEADEAFRGARVEVAPDGEAAVVRALVVGDSRDATSVAAVEAIRSDIAPSALSG
                     TDSRVLVTGETAETIDYRELTSSWLPIVFVFVLGLSFVLLAFAFRTIVMPVVAIALNL
                     LSVGAAYGLLLLVFVKGYGNEIFGFVQVEVIAAWLPLFLFAVLFGLSMDYTVFLLSRI
                     RERHAGGDPMKDAVAHGVASTARIVTGAALIIIIVFVGFAAGDQVEFQQMGFGVAVSL
                     LIDATLIRLVLMPAVLAVLGERAWYLPGWLEWLPHVEIEGHAGPRPPESAGPGS"
     gene            2405..3049
                     /locus_tag="AC150V_10225"
     CDS             2405..3049
                     /locus_tag="AC150V_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR3763334.1"
                     /translation="MKRGTATRERLLDAAITLFAEHGYAATTVGAIEEAAGLAPRSGA
                     LYQYFESKRALLDATLQREMATLAELPSVMSMLPLGDIRAELTLLARWNLQSLERRSR
                     LSRFIRREGDRLPADVREELYDQLVREPYGQLSEWLGDRASALGVHIEDPYALLVILV
                     ESMSSYRTLRERFGEVPGDVDDDRFVATWVELCLAYAVTRASPTGSDDVVESAS"
     gene            3046..3363
                     /locus_tag="AC150V_10230"
     CDS             3046..3363
                     /locus_tag="AC150V_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR3763335.1"
                     /translation="MSEQEKDVRKPAPAPKTPPKTDPAAADDQRDTLDWIRYGLIGLA
                     ILYALILLLLNSDRIKIDFLFFSANARLWILLLLTFVLGGIAGFVGARIYGREKPETK
                     RKS"
     gene            complement(3360..4145)
                     /locus_tag="AC150V_10235"
     CDS             complement(3360..4145)
                     /locus_tag="AC150V_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR3763336.1"
                     /translation="MKRTGLMLALVAAVVSVGADAKPRPTPRAAELVVALDLAGSGLQ
                     GGAVRGSQVVLAKGFEVDLARAVARKIGVKKVRFVNVPRERITAPGKKRWDFAIGQLS
                     GPVRGVEYSSPYLQKSHAVVVRRGLRKPTKLGALRGLQLCAEKRTSSATVVVGRVRPA
                     LPALYVADTAAMLRRVQTGACDAAVLETTELRSALAGKADRVGPVAGRIETNVSYAIA
                     LPKQSSLTQKVNEALKRLRADGTLARLRSEWLGGDPLRLPILR"
     gene            complement(4142..5200)
                     /locus_tag="AC150V_10240"
     CDS             complement(4142..5200)
                     /locus_tag="AC150V_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR3763337.1"
                     /translation="MLRLRANMRNIARSAALAAVAGVALVLLAPAANAAESAGTGVWF
                     VRDGALVSSERRLASISAAVRALVAGPTAKERAAGLRSAVPAGTRIREISVRRQVVTV
                     DLSARFAAGRNDAALASRIGQLVKTVGGLPGVRGVRVRIEGGIPIGLFPGYDLRRALT
                     PAAVKPEPRPGIREFQTLLTDLGFMGPGGVSGEFDDRTAVAVLAFQKWARLPRTSELD
                     PATARALLRSTRPNPAPYAAGQRVEVLLDRQLAMLVEGGRVLRTIHVSTGAYNRTPTG
                     TFQVFRKERMSWSVPFSVWMPWASYFVGGIAFHEYSSVPTYPASHGCIRVNRYDAPRL
                     YEFASYGTQVTVLSGVQA"
     gene            complement(5233..5709)
                     /locus_tag="AC150V_10245"
     CDS             complement(5233..5709)
                     /locus_tag="AC150V_10245"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0050896 - response to stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3763338.1"
                     /translation="MAGKIVVGVDGSDHGRAALRFAAEEAKVRGATLVAAHVWAFAPP
                     VAVGTTDVMAMPAGNLPAELDAERDVATRVLGESIEEALGSTPDIPIDRLLIEDAPGE
                     GLVRSAEDAALLVVGSRGRGSFASAVLGSVSHYVVQHSPCPVVIVRAEHDPHGAEA"
     gene            5777..6361
                     /locus_tag="AC150V_10250"
     CDS             5777..6361
                     /locus_tag="AC150V_10250"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR3763339.1"
                     /translation="MERRLIIGMTGASGMPYGVRLLQILRDVPDVETHLVMTEPAKLN
                     VLVETDLRTSDVEALADVVHSVKNISATIASGSVRTEGMIVAPCSMRTLASIVHSYAD
                     NLLVRAADVTLKERRRLVVMPRETPLSIGHCKLLYEASLQNVIVCPPMPGFYMQPKTI
                     EDLVDASVGRVLDLFDIDTGLVRRWAGIPGREED"
     gene            complement(6386..6832)
                     /locus_tag="AC150V_10255"
     CDS             complement(6386..6832)
                     /locus_tag="AC150V_10255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HIR3763340.1"
                     /translation="MKDVQQAATVVWQGSVARGVGELTGGSGALGPLAVDMPTRIGEA
                     AGKTTPEELLAAAQASCFVTALGSALAGKKLPPERLEVTATVTLALSGERPDIPSIEL
                     EVTGVVPGADQATFAAIVEETKADGCLISRVLTGGTVKISATSTLA"
     gene            6917..9607
                     /locus_tag="AC150V_10260"
     CDS             6917..9607
                     /locus_tag="AC150V_10260"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR3763341.1"
                     /translation="MSTPARHSTTAPSPSTGEAAQQTAERLGGVVESYRRLADVFHDV
                     LSEQSTDALLERIADTVGELIPHDDLAIYEADERRRELKAVFARGNWAEEVLADDPFA
                     FGDGITGRAVEQRKPLLANRAELDPGVRYVANTPREPESLIAVPLIARGRVKGTLNVY
                     RVGLKEFTYEEFRLAVRFGDAAALALDNAHIRASLEHQAQTDSLTGLYNHRAFHEHVR
                     LALVRASSAHESVAVVMLDLDNFKRVNDVYGHGSGDAVLAEIADHLRAGVRDTDAVCR
                     IGGEEFAIVVPGGTSDVARGLAERISERIEGAQFDVPDQVTVSSGVAVGPDHAANPRE
                     LIACAEVAMMTAKARGRGQVVVFDEESSARPDKVRNDDARSIAHLKMLQALSAKVARL
                     EDVAEIGEAVVTELRQLIDYHNCRVFLREGENLRLVAFHGDLSTCGGAKPVVRSTTVG
                     VGITGRVAATGEPALVGDAANCEYAHHIEGTEQIEESVLAVPLTQRTSVVGVIVVSKL
                     GLDQFDVDDQRLLEVMAGHVAGALVNARLYESTRREAEGAKALLGLSRELSASTDIEE
                     LGERVAAESARILGAEHASVWLPDAETGGLRCGGCWSTDAARRAAVSEMALPARVAER
                     LVDGREPHVIVPGDYENLVDVGALPLGSGVYAIAPFPVDDRWGAVAVAADTPGEYDAR
                     ALELLGEIADQTRLALGIALGFRILESTFLATVEALANALEAKDAATSFHARWITDMA
                     VRVGQELGLDQKGLKRVELGALLHDIGKIGIPSAILSKPGPLTAAERSLIETHPEVGE
                     RILEPIQQLADVRPVVRACHENWDGTGYPDGLKGDEIPLESRIIFACDAFYAMISDRP
                     YRPRLSVDEARRRLTDGASRQFDPSVVEVCLRILDELDEP"
     gene            complement(9728..10495)
                     /locus_tag="AC150V_10265"
     CDS             complement(9728..10495)
                     /locus_tag="AC150V_10265"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763342.1"
                     /translation="MRVLPVTRGVAWRTLKVVFTSPSLFLPAIAFPLFFFIAFAGGLA
                     QVENVPGFDYPSGYTGFQFVFVLLQSAAFGGVFTGFGIARDFEGGFAKRLLLASPSRT
                     GIVLGYMVAATCRWFVTASILTVVALVAGMEVFGSTSQVVGLYTLALLVNVTGFLWAG
                     GVAMRFRTIQAGPIMQMPVFLLLFFAPVYVPLTLLDGWIKGVATVNPITYLLESGRGF
                     LAGSPVYVGLAFALGLLLVLGFAFWARGGLRSAEAAG"
     gene            complement(10492..>10709)
                     /locus_tag="AC150V_10270"
     CDS             complement(10492..>10709)
                     /locus_tag="AC150V_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763343.1"
                     /translation="MNAPRDLLQVEWVRTLATINPVSYLIECVRSLIITGWDWKALGL
                     GFGIAIVIAVLSLALAAVALRRRLART"
BASE COUNT         1855 a         3681 c         3655 g         1518 t
ORIGIN      
        1 tcaacccttc gtccgtgtga atccggacga ggaacgcgtc ttgcgtgccg tcggcgcgcg
       61 tcgtgtcgac gtcgggcagc cgcaggacga tcggctcgac gcgcgtgatc ttcacgagcc
      121 tgggcccgcg ctctcgggcg gccggggccc ggcgtggccc tcgatctcga cgtgcggcag
      181 ccactcgagc cagccgggca ggtaccacgc gcgctcgccg agcaccgcga gcaccgccgg
      241 catcagcacc aggcggatca gcgtagcgtc gatgagcaac gagacggcga cgccgaatcc
      301 catctgctgg aactcgacct gatccccggc tgcgaagccc acgaagacaa tgatgatgat
      361 cagcgccgcg ccggtcacga tgcgcgccgt cgaggcgacg ccgtgtgcga cagcgtcctt
      421 catcggatcg ccaccggcat gccgttcgcg gatccggctg agcaggaaga ccgtgtagtc
      481 catcgacagc ccgaagagca ccgcgaagag gaacagcggc aaccaggcgg cgatcacctc
      541 gacctgcacg aagccgaaga tctcgttccc gtaccccttc acgaagacga gcagcaggag
      601 tccgtacgcg gccccgaccg acaagaggtt gagcgcgatc gcgaccacgg gcatcacgat
      661 cgtccggaat gcgaacgcga gcaagacgaa gctcagcccg aggacgaaga cgaacacgat
      721 cggcagccaa ctcgacgtca gctcgcggta gtcgatcgtc tccgccgtct cccccgtgac
      781 cagcacccga gaatcggtgc cggagagcgc ggacggcgcg atgtcggagc ggatcgcctc
      841 cacggccgca accgacgtcg cgtcgcggct gtcgccgacg acgagcgcgc ggacgaccgc
      901 cgcctcaccg tcgggtgcga cctcgacgcg cgcgccgcgg aaggcctcgt cggcctcgac
      961 ttgctcggcg atctcggcca ggcccgcact gatttcaggt gacgcgacat cgccttcgac
     1021 gacgacctgc acttcgtcca cggtggcgac gccgaactcc tcctcgagcg cgacgaagcc
     1081 ctgcttggcc gcaaagcggt cgggcagcgt gcggatgccc gagaagcccg tctcgaagtc
     1141 gagcaccggg atcgcaaccg cgagcaggac ggcgaccgac aggcccgccg aaacccaggg
     1201 aacacgcatc acggcgcgta cggcacgcga ccagagagcg ccctcggtgc ccgcgctccg
     1261 ctcgatccgg cgaccgagcc atgggatgcg tagtgcgttc acgcggtcgc cgaggaggct
     1321 gagcaccgcg ggcaagaggg tcagcgcgac cacgacggag acgatcccga cgagaatcgc
     1381 gcccgcagcg aggcttcgca ggatcgtgtc cggcacgagg accatcccga ccatcgcgag
     1441 cacgaaggcg gtgccgctga agaggactgc cctgctcgcg gtcgagccgc acacggcgat
     1501 cgcgtccagc ttgtcgcgcc cccggtttcg ctcctcacga aaacggctga cgatgaagag
     1561 cgcgtagtcg atgccgagcg cgagtcccat gccggtgagc atgttcacga cgaagatcga
     1621 gagctcgaaa gcctgaccga ggagcgcggt cagcgcgagg gcgaccacga tcgacacgag
     1681 agctaccagc accggcacga gtcccgcgac gagcgagccg aagacgagga tcaggacgat
     1741 cagggcggcc gggagcccga actggaactc gcccttccgc aagtcctcct cggatagctc
     1801 ggtgtagtcg tgatcggccg tgaactcgcc cgtgatcgcc gtctcgaagc cgtccttctc
     1861 ggccgcatcg acggtcgcga tcacgtcccc gatcttgtcc tccccctcgc cagtgagtgc
     1921 gaccgcgatc agcgccgcat cgcgatcggg ggagacgagc gacgggtcgt cggtgccgta
     1981 gaaggtgcgg gcctgccacg cgtagccgct gctctcgagc tctcctgcca gggcgacgac
     2041 tgcgcgctcg aacatcgggt cgtcgacctt cacgtcggcg gagcgcacga tcacgacctc
     2101 gttgaccacg tcggccggat cgaaggggaa gtgctgcccg atgagatcgt aggcgcggta
     2161 cgactccggg tcgttcgtca tctcgccctc ggtcgtcagt gacgagccaa gcagagtggc
     2221 gatcacggcg agcgagacga cgatcgcgac tccccacgcg gccaacgtgc gcttcggatg
     2281 cgcggcggac gcgcgcgcca acctgccggt caggtctctc atcggttccc ccgcacctgg
     2341 ttcatgcccg agcacgttat cacaacatct gtagtgagaa gatacactga tcgttgtgga
     2401 acgaatgaag cgaggtactg cgacccgcga gcgactgctc gacgccgcga tcacgctgtt
     2461 cgccgagcac ggctacgccg ccacgacggt cggtgcgatc gaggaggccg ccgggctggc
     2521 gccgcgatca ggcgccctgt accagtactt cgagagcaag cgcgcacttc tcgatgcaac
     2581 gctgcagcgt gagatggcaa cgcttgcgga gttgccgtcc gtgatgtcga tgcttccgct
     2641 gggcgacatc cgagccgagc tgacactgct cgctcgctgg aatctccagt ctctcgaacg
     2701 caggtctcga ctcagccgct tcatccggcg ggagggcgac cggcttccgg ccgacgtccg
     2761 tgaggagctc tacgaccaac tcgtcagaga gccgtacggg cagctctcgg agtggctcgg
     2821 cgatcgcgca agcgcgcttg gggttcacat cgaggatccg tatgcgctgc tcgtcatctt
     2881 ggtggagtcg atgagctcct atcggacgct gcgcgagcgc ttcggagagg tcccgggcga
     2941 cgtcgacgat gaccggttcg tcgctacctg ggtcgagcta tgcctcgcct atgccgtcac
     3001 ccgcgcgtcc ccgactgggt cggacgacgt tgtagagtct gcttcgtgag cgagcaggaa
     3061 aaagacgttc ggaagccggc cccggcgccc aagacgccac ccaagaccga tccggcggcc
     3121 gccgacgatc agcgggacac gctggactgg attcggtacg gcctgatcgg gctcgcgatc
     3181 ctctacgcgc tcatcctcct gctgctgaac agcgacagaa tcaagatcga cttcctcttc
     3241 ttcagcgcga acgcgcgcct ctggatcctc ctgctcctca ccttcgtgct cggcggcatc
     3301 gccggcttcg tcggggcgcg catctacggg cgggaaaagc ccgaaacgaa gcgtaagagc
     3361 tagcggagaa tcggcaggcg aagcgggtcg ccgccgagcc attcggagcg aagacgggca
     3421 agggtcccgt cggcgcgtag tcgcttcagc gcctcgttga ccttctgcgt cagcgagctc
     3481 tgcttcggca gcgcgatcgc gtacgagacg ttcgtctcga tgcgcccggc aaccggtccg
     3541 acgcgatctg ccttgccggc gagcgccgag cgcagttccg tcgtctcgag gacggccgca
     3601 tcgcaggccc ccgtctgcac acggcgaagc atggcggccg tgtcggcgac gtacagcgcc
     3661 ggcagcgccg ggcgaacgcg gcccacgacc accgtcgccg aggacgtccg cttctcggcg
     3721 cagagctgca gccctcgcaa cgcaccgagc ttcgtcggct tgcgcaggcc gcgccgcacg
     3781 acgacggcgt gggacttctg caggtagggc gaggagtact cgacgccgcg cacggggccg
     3841 ctgagctgac cgatggcgaa gtcccagcgc ttcttccccg gagccgtgat tcgctcgcgc
     3901 gggacgttga cgaatctgac cttcttgaca ccgatcttgc gcgccacggc gcgggcgagg
     3961 tcgacctcga agcccttcgc cagcacgacc tgcgagccgc gaacggcgcc cccctggagc
     4021 ccggagccgg ccagatcgag ggcgacgacc agctcggcgg cgcgcggcgt cggccgcggc
     4081 tttgcgtctg cgccgacgct cacgacggcg gcaaccagcg cgagcatcaa tccagtccgt
     4141 ttcatgcctg cactcccgac agaacggtga cctgcgttcc gtacgaagcg aactcgtaca
     4201 gacgaggcgc gtcgtagcgg ttgacgcgaa tgcagccatg ggacgccgga taggtcggca
     4261 ccgaggagta ctcgtggaag gcaattccgc ccacgaagta gctggcccag ggcatccaga
     4321 ccgagaaagg gacggaccag gacatccgct ccttgcggaa gacctggaac gttccggtcg
     4381 gcgttcggtt gtaggcgccg gtggaaacgt ggatcgtgcg gaggacccgt ccgccttcga
     4441 cgagcatcgc gagctgccgg tcgagcagga cttcgacccg ctggccggcc gcgtacgggg
     4501 ccgggttggg tcgcgtggaa cgaagcagcg cgcgggccgt ggccggatcc agctcgctgg
     4561 ttcgcggcag ccgggcccat ttctggaaag cgagcaccgc cacggccgtg cgatcgtcga
     4621 actcaccaga gacgccaccc gggcccatga agccgagatc ggtgagcagg gtctggaact
     4681 cacgaatccc cggccggggc tcgggcttca cggcggcagg tgtgagggcc cgccgcaggt
     4741 cgtagcccgg aaacaggccg atcgggatac cgccttcgat ccggacgcgg acgccccgca
     4801 cgcccggcag gccgcccact gtcttcacga gctggccgat ccgagaggcg agtgctgcgt
     4861 cattgcgtcc ggccgcaaag cgggcggaga ggtcgaccgt caccacctga cgtcgaaccg
     4921 agatctcacg gatgcgcgtc ccggccggca ccgccgagcg aaggcccgcc gcacgctcct
     4981 tcgccgtcgg gccggcgacg agcgcccgta cggccgcgga gatcgaagcg agccgtcgct
     5041 cggacgagac gagggcgccg tcgcgcacga accagacacc ggtgccggcg ctctcggccg
     5101 cgttcgctgc tggcgcgagg aggacaagcg cgacacccgc cacggcggcg agggccgccg
     5161 accgagcgat gttccgcatg ttcgcgcgca ggcgcaacat gctctccact atcggacgga
     5221 gcccttgaat ccttaggctt cggcgccgtg cgggtcgtgc tcggcccgga cgatcacgac
     5281 cgggcacgga gaatgctgaa cgacgtagtg actgacggag ccgagcacgg ccgacgcgaa
     5341 gctgccacgg ccccgcgagc cgacgacgag gagcgcagca tcctcggcgg aacgaacgag
     5401 gccctcgccg ggggcgtctt cgatcagcag gcgatcgatc ggaatgtcgg gagtcgatcc
     5461 cagggcttcc tcgatcgact ccccgagcac ccgggtggcg acgtcccgct cggcgtccag
     5521 ctcggccggc agatttcccg cgggcatcgc catcacgtcc gtcgtaccga ccgcaaccgg
     5581 cggagcgaag gcccagacat gggcggcaac cagcgtggca ccgcgtacct tggcctcctc
     5641 ggcggcgaag cggagcgcgg cacggccgtg gtcggagccg tcgacgccga caacaatctt
     5701 ccctgccacg gccgaagcct agctctcgcg gggcgagggc ggagaagatc ggccttcccc
     5761 tacgattgac cgtcagatgg agcggcgact catcatcgga atgaccgggg cgagtggcat
     5821 gccctacggt gtccgcctgc tccagatcct gcgcgatgtg cccgacgtcg agacccacct
     5881 cgtcatgacc gagcccgcga agctcaacgt cctcgtcgag accgatctcc gcacgagtga
     5941 cgtcgaggca ctggccgacg tcgtgcacag cgtcaagaac atctccgcga cgatcgccag
     6001 cggctctgtc aggacggagg gcatgatcgt cgcgccgtgc tcgatgcgca cgctcgcctc
     6061 gatcgtccac tcgtatgccg acaatctgct cgtccgggcc gccgacgtga cgctgaagga
     6121 acgccggcgt ctcgtggtca tgccgcgcga gacgcccctg tcgatcggcc attgcaagct
     6181 gctctacgag gcctcgttgc agaacgttat cgtctgcccg cccatgccag gcttctacat
     6241 gcagccgaag acgatcgagg atctcgtcga tgcctcggtc ggacgcgtgc tcgacctctt
     6301 cgatatcgac accggactcg tccggcgctg ggccggcatc cccggccgcg aagaggatta
     6361 acgacccgcc gcgctcaccc ggcgctcagg cgagggtgct cgtggccgag atcttcaccg
     6421 tgccgccggt gagcacccgg gagatgagac agccgtcagc cttcgtctcc tccacgatcg
     6481 ccgcgaaggt cgcctggtcg gcgcccggaa cgacacccgt cacctcgagc tcgatcgaag
     6541 ggatgtcggg ccgctcgccg gagagggcga gtgtcacggt cgcggtcacc tcgaggcgct
     6601 cgggcgggag cttcttgcca gcgagggccg agccgagcgc ggtcacgaag cagctggcct
     6661 gagcggctgc cagcagctcc tcgggcgtgg tctttccggc cgcttccccg atgcgggtgg
     6721 gcatgtcgac cgcgagcggg ccgagcgctc cgctgccgcc ggtgagctcg ccgacgccgc
     6781 gggccaccga gccctgccac acaaccgtcg ccgcttgctg cacgtccttc acgcctctcc
     6841 cttcgtcgcg ctgcgggtga aggacgttag cggcttaagt cgagccgcgc cggcgacgat
     6901 gagtgaccca agcccgatga gcacccctgc gcggcattcg acgacggctc cctccccttc
     6961 caccggagag gcggcccagc agaccgccga gcggctcggc ggagttgtcg agtcctaccg
     7021 tcgcctcgcc gacgtctttc acgacgtcct ctcggaacag agcaccgacg cgctgctgga
     7081 gaggatcgcc gataccgtcg gcgagctcat cccgcacgac gacctcgcga tctacgaggc
     7141 cgacgagcgc cgccgcgagc tgaaggccgt cttcgcacgt gggaactggg ccgaggaagt
     7201 gctggcggac gatccgttcg cgttcggcga cgggatcacc ggcagggcgg tagagcagcg
     7261 caagccgctg ctcgccaatc gggcagagct cgacccaggt gtccggtacg tcgccaacac
     7321 accgagggag cccgaatcgc tgatcgcggt gccgctgatc gcgcgcggac gggtcaaggg
     7381 gacgctgaac gtgtaccgcg tcggcctgaa ggagttcacg tacgaggagt tccgccttgc
     7441 cgtgcgcttc ggggacgcgg cggcgcttgc cctggacaac gcgcacatcc gggcgagcct
     7501 cgagcaccag gctcagacgg actctctcac gggcctctac aaccaccgcg cgttccacga
     7561 gcacgttcgg ctcgcgctcg tgcgtgcctc gtctgcgcac gagtcggtcg ctgtggtgat
     7621 gctcgatctc gacaacttca agcgcgtcaa cgacgtgtac gggcatggca gcggggacgc
     7681 cgtgctcgcc gagatcgccg accacctgcg tgcgggcgtg cgagacaccg acgccgtctg
     7741 tcggatcggc ggcgaggagt tcgccatcgt cgtgccgggc ggcacgtcgg acgtggcacg
     7801 cgggcttgcc gaacgcatct ctgagcggat cgaaggggcg cagttcgacg tcccggatca
     7861 ggtgacagtc tcctcgggcg tcgcggtggg ccccgaccac gcagccaacc cgcgcgagct
     7921 catagcctgc gccgaggtcg cgatgatgac ggccaaggcg aggggccgcg gccaggtcgt
     7981 cgtcttcgac gaggagtcgt cggcgcgccc ggacaaggtc aggaacgacg acgcgcgatc
     8041 gatcgcccat ctcaagatgc tgcaggccct gtccgccaag gttgcgcggc tcgaggatgt
     8101 cgcggagatc ggcgaagccg tcgtgaccga gctgcgccag ctgatcgact accacaactg
     8161 ccgcgtcttc ctgcgggaag gcgagaatct ccgtctggtc gccttccacg gcgatctctc
     8221 gacctgcggg ggcgcgaagc cggtcgtccg gtcgacgacc gtcggagtgg gcatcaccgg
     8281 acgtgtggca gcgaccggcg agccggccct tgtcggggac gcggcgaatt gcgagtacgc
     8341 ccaccacatc gaaggcaccg agcagatcga ggagtcggtt ctcgcggtgc cgctgacgca
     8401 gaggacgagc gtcgtcggcg taatcgtcgt gtcgaagctc ggtctcgatc agttcgacgt
     8461 agacgaccag cggctgctcg aggtcatggc gggtcacgtc gccggggctc tcgtcaacgc
     8521 tcgcctctac gagagcacgc gccgggaggc ggagggtgcg aaggccctgc tcggcctctc
     8581 ccgcgagctc tcggcgtcca ccgacatcga agagctcggc gagcgcgtcg cggccgagtc
     8641 cgcccggatc ctcggtgcgg agcacgcatc ggtctggctc cccgacgcgg agacaggcgg
     8701 tctgcgctgc ggcggctgct ggagcaccga tgcggcgcgc cgggcggcag tgtccgagat
     8761 ggcactaccg gcgagggttg ccgaacgcct ggtcgacggg cgcgagccgc acgtgatcgt
     8821 tcccggcgac tacgagaacc tggtcgacgt cggcgcgctg cctctgggga gcggcgtcta
     8881 cgcgatcgcc cccttcccgg tcgatgaccg ctggggtgcc gtcgccgtag cggccgacac
     8941 gccgggcgag tacgacgcga gggcgctcga gctgctcggc gagatcgccg accagacccg
     9001 gctggccctc ggcattgccc tcggctttcg aattctcgag tccacgtttc tggcgacggt
     9061 cgaggcgctg gccaatgccc tggaggcgaa ggatgccgcg acgtcgttcc acgcccgctg
     9121 gatcaccgac atggccgtgc gggtcggaca ggagctcggc ctcgaccaga agggcctgaa
     9181 acgggtcgag ctcggcgcgc tgctgcacga catcggcaag atcggcatcc cgtcggcgat
     9241 cctcagcaaa cccggtccac tgacagccgc ggagcggagc ctcatcgaga cgcacccgga
     9301 ggtcggcgag cgcatcctgg agccgatcca gcagctcgcg gacgtgcgcc ctgtcgtccg
     9361 agcctgccac gagaactggg acggcaccgg ctaccccgac gggctgaagg gggacgagat
     9421 cccgctggag tcccggatca tcttcgcgtg cgatgccttc tacgcgatga tcagcgaccg
     9481 accgtaccgg ccgcggctgt ccgtcgacga ggcgcgccgg aggctcaccg acggcgccag
     9541 taggcagttc gatcccagcg tggtcgaggt ctgccttcgc atcctcgacg agctcgacga
     9601 gccataggga agggagggga acccacggtt cccccacgag ccgcctccgc tcctcgccgg
     9661 aaacgcccgc gggacttcag cctgcggtga ttccgcctct gccgcatccc gaggcagccc
     9721 gtagtctcta gccggccgcc tccgccgagc gcaggccgcc gcgcgcccag aaggcaaagc
     9781 cgaggacgag taggaggccg agggcaaagg cgaggccgac gtagacgggg ctgcccgcca
     9841 gaaagccgcg accggactcg agcaggtacg tgatcgggtt gacggtcgcg acgcccttga
     9901 tccagccgtc caagagcgtg agcggcacat acacgggagc aaagaagaga agcaagaaga
     9961 ccggcatctg catgatcggg ccggcctgga tcgtccggaa cctcatcgcg accccgcctg
    10021 cccagaggaa gccggtcacg ttcacgagga gcgccagtgt gtagaggccg accacctggg
    10081 aggtcgagcc gaagacctcc atgccggcga ccagcgcgac gaccgtgagg atcgaggcgg
    10141 tcacgaacca ccggcacgtg gcggccacca tgtagccgag tacgatgccg gtacggctcg
    10201 gcgaggcgag caggagccgc ttcgcgaagc cgccctcgaa gtcccgagcg atgccgaagc
    10261 cggtgaagac gccgccgaaa gccgccgact gcagcaggac gaagacgaac tggaaccccg
    10321 tgtagccgga cgggtagtcg aaacccggaa cgttctccac ctgcgccagg cctcccgcga
    10381 aggcgatgaa gaagaagagc gggaacgcga tcgcaggaag gaagagcgag ggactggtga
    10441 agacgacttt gagcgtccgc cacgcgacgc cccgggtgac cggcagcact ctcatgtgcg
    10501 cgccagcctt cgacggaggg cgaccgctgc gagcgccagc gagaggactg cgatgacgat
    10561 cgcgataccg aacccgaggc cgagcgcctt ccagtcccac ccggtgatga tcaggcttcg
    10621 cacgcactcg atcaggtacg agaccgggtt gatcgtggcg agcgtccgga cccactccac
    10681 ctgcagcaag tcccggggcg cgttcatcg
//
LOCUS       DBNGNK010000347         6182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_417669,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000347 DBNGNK010000000
VERSION     DBNGNK010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_417669"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..465)
                     /locus_tag="AC150V_10275"
     CDS             complement(4..465)
                     /locus_tag="AC150V_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR3763344.1"
                     /translation="MSHVYCLECGFQNPEAANYCSRCGAFLRAESAGETTLSIAPDEV
                     GNEGVARDEQEVGRPALVVRSGGGRAGESFRASGPQTLIGRSPECDIFLDDVTVSRRH
                     AELVADGNRYTIRDLGSLNGTFVNRKRIESADLDDDDEVQIGKYRMAFLRR"
     gene            complement(462..1010)
                     /locus_tag="AC150V_10280"
     CDS             complement(462..1010)
                     /locus_tag="AC150V_10280"
                     /inference="COORDINATES: protein motif:HMM:NF046924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiG family protein"
                     /protein_id="HIR3763345.1"
                     /translation="MSNPSERQTVEPIPDLATISDDELDGMIRGLEEEEERISLRRRV
                     LHGQIDLLRAERNGRLREQVGSGEGETTDPGLVAATVVRDETAGSDEERPVPEEVDAL
                     PDLAALSTDELRALIRELDLEEDEMSLRRRVLHGRIDILRAERQLRARGSAAGEPSHV
                     DIDRLKEILAARLVARPSDEST"
     gene            complement(1047..3569)
                     /locus_tag="AC150V_10285"
     CDS             complement(1047..3569)
                     /locus_tag="AC150V_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR3763346.1"
                     /translation="MAGGEGTRLRPLTSNQPKPMVPIVGKPCIEHILELLRTHGFREI
                     VVTLAFMPQAIRGHFGDGEAMGLTIDYSIEEQPLGTAGSVALASHRLDDTFLVISGDA
                     LCDVDLTELVAAHRRTGAAVTIGLKSVENPLEFGIVVTDDEGRVERFLEKPSWGQVFS
                     DTINTGIYVIEPEVLRHIPTGQPHDFSKELFPLLLDMGRPIYGHVMDGYWQDIGNLDQ
                     YRQANFDALDGDVRLSIKGIRLRGNVWIGEGVNIDDVEHLEGPAYVGANCRIAPDASV
                     GAYTVLGQGVTLRERGRVARSIIDASTYIGRSALVEGAIVGRSCDIRNHVRVHEGVAI
                     GDEVTIGPEASIMPGVRVYPFKEVETGAQIHESLIWETRVSQRVFGVDGVTGLVNVDL
                     TPEDAVRLAGALGSALDSGARVVASRESPDACRMIQRAMIVGLTSAGVDVADLRISPS
                     AITRHVLKTQGLAAGVHVARSAVDPELVQIRVFEFPGTQISSSLQREVERHYTRRELR
                     RAAFEEIGETTYPTRVRESYAQDLLDTLDIAAIRDRGARIAIDYGYSAASFVLPLIVG
                     SLGVEAVTAHGFFAEAADGPSAPAPESATHVARIVTAIGADLGVVFDAGAERIVLVDE
                     TGTVIPPDRALLLYVKLLAATGRSGTIAVPVTVTSEVDALVADTQLDIVRTPHSLSDL
                     TRVAAGEQVVFAGSVSGGYAFPDVVPGYDAVTSLCKLLELLAAAGDQPLSHLVAALPR
                     PTLVRRSLACPWARKGLVMRLLNESLADRELDLLDGLKAYDSRGWVQVLPDPYEPVVH
                     VFAEGETDEESAALADELEGEITAIVEGEHVAARSEGVETSS"
     gene            complement(3581..4135)
                     /locus_tag="AC150V_10290"
     CDS             complement(3581..4135)
                     /locus_tag="AC150V_10290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR3763347.1"
                     /translation="MSTSTISAPRRQLPNALTILRFAAIPIFIVLYINAGDGAAWWAG
                     LVFAVAAATDQLDGWLARRWKVESEFGKVADPLADRLMIGVAAVLLWIDGRLPWPAAV
                     IVLGRDLLLVLGYRFVVPRGYDFEVSFLGKLATWVLYFSLLCVIVTEKGTAWPLWLFW
                     IGVALAVAAAVQYMLKARREVGRE"
     gene            4178..4567
                     /locus_tag="AC150V_10295"
     CDS             4178..4567
                     /locus_tag="AC150V_10295"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR3763348.1"
                     /translation="MHRWTSHTGEEQLEIEADTPAGVFAEAVDAFGRSVELAKDGAPA
                     AHAVHLEGREPAALLVQLLNELVFLADTEGFVPDRSELVLEDAVLRGRILGRSTTVRP
                     LVKAATYGGLAFEEFDGFWRARVVLDV"
     gene            4560..5978
                     /locus_tag="AC150V_10300"
     CDS             4560..5978
                     /locus_tag="AC150V_10300"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013905170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR3763349.1"
                     /translation="MSDLRQIDETLWEIPAESRADMRVPARVFADRDIVAAIEGDLSL
                     EQLQNVTTLPGIVGAAIAMPDIHQGYGFPVGGVAATELPDGVVSPGGVGYDINCGVRL
                     LSLPLSERDLGGRREALVHDISRSVPAGAGKHGVLELDDLTLDSVLSEGPRALPNVRP
                     RDIEHTESGGRLEGADPTAVSKRAHARGRAQLGTMGSGNHFVELQMIESVLDDRVASA
                     FGLVAGQVTVLIHSGSRGLGHQVCTDYVRQMDDAQERYGFALPDRQLACAPASSPEGA
                     AYLAAMAAAANFAWANRQVIADSVRAAIRSVLGDDAADATNQVYDVAHNVAKRERHLG
                     KDVLVHRKGATRAFPPGSTELPGEYATVGQPVFIPGSMGTASFVLVGEPAAMEKSFGT
                     VCHGAGRVLSRTAARKRISGHDLRRELEGRGIVVRSPSARGLAEEAPFAYKDVDAVVR
                     VVEQAGLARRVARLVPLGVVKG"
     gene            6008..>6182
                     /locus_tag="AC150V_10305"
     CDS             6008..>6182
                     /locus_tag="AC150V_10305"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR3763350.1"
                     /translation="MDVTETTFEAAVIERSRELPVIVDFWAEWCGPCHALTPVLESAV
                     SERSGAVELVKVDV"
BASE COUNT          970 a         2112 c         2094 g         1006 t
ORIGIN      
        1 tcgtcatcgg cgaaggaatg ccatccggta tttgccgatc tgcacctcgt cgtcatcgtc
       61 gaggtcggct gactcgattc gcttgcggtt gacaaatgtg ccgttcaggc tgcccaggtc
      121 gcggatcgtg tagcggttgc cgtcggcgac gagctctgcg tggcgccgcg acacggtgac
      181 gtcgtcgagg aagatgtcgc actccggcga gcggccgatc agcgtctgcg gcccggacgc
      241 gcggaagctc tcgccggcac gaccgccccc ggagcgcacg acgagagccg gccgcccgac
      301 ctcctgctcg tcgcgtgcga caccttcgtt gcctacctcg tcaggggcga tgctcagagt
      361 cgtctcgcct gcagactcgg cgcggagaaa agccccgcag cgcgagcagt agttcgcggc
      421 ttccgggttc tgaaagccgc actcgaggca gtagacgtgg ctcacgtcga ctcgtcgctc
      481 ggccgcgcga ccaagcgcgc ggcgaggatc tccttcaagc gatcgatatc gacgtgtgag
      541 ggctcgccgg cggccgagcc gcgggcgcga agctggcgct cggcgcggag gatgtcaatc
      601 cttccgtgca gcacgcggcg ccgcagcgac atctcgtcct cctcgagatc cagctccctg
      661 atcagcgcgc gaagctcgtc tgtcgagagc gcggcgagat cggggagcgc gtccacctcc
      721 tcgggcacgg ggcgctcttc gtcgctgccc gccgtctcgt cgcgcacgac ggttgcggca
      781 acgaggccgg ggtcggtcgt ctccccttcc ccgctgccca cctgctctcg caggcggccg
      841 ttccgctcgg cacggagcag gtcgatctgc ccgtggagca cccgacggcg cagcgagatc
      901 cgctcctcct cttcctcgag cccgcggatc atcccgtcca gctcgtcgtc ggagatcgtc
      961 gccagatcgg ggatcggttc cactgtctgc ctttcgctcg ggttgctcac ctcgattgaa
     1021 gcatcagccg aggccagggg tcaacctcaa cttgaggttt ctactccctc acttcgcgcc
     1081 gcgacgtgtt ctccctccac gatcgcggtg atctccccct cgagctcgtc cgccaaggcg
     1141 gcggactctt cgtccgtctc cccctcggcg aagacgtgca ccacgggctc gtacggatcg
     1201 ggtagcacct gcacccagcc acgcgaatcg taggccttca gtccgtcgag caggtcgagc
     1261 tcccgatcgg cgagcgactc gttgagaagg cgcatcacga ggcctttgcg cgcccagggg
     1321 caggcgagcg agcgccgcac caacgtcggg cgaggcagag ccgcgacgag gtgcgataag
     1381 ggctgatcgc cggcggccgc cagcagctcc agcagcttgc agaggctcgt gacggcgtcg
     1441 tagccgggta cgacgtcggg gaaggcgtag ccaccgctga ccgagccggc gaacacgacc
     1501 tgctcgccgg ctgcgacccg ggtgaggtcg ctgagcgagt gcggcgtgcg cacgatgtcg
     1561 agctgagtgt ccgcgacgag cgcgtcgacc tcactcgtca cggtgacggg aaccgcgatc
     1621 gttccgcttc tgccagttgc ggcgaggagc ttcacgtaga ggagcagcgc ccggtcgggt
     1681 ggaatgaccg tgccggtctc gtcgacgagc acgatccgct ctgcgcccgc gtcgaatacc
     1741 acacccaggt cggcgccgat cgccgtgacg atgcgggcca catgcgtggc gctctccgga
     1801 gcgggtgccg agggaccgtc ggccgcctcc gcgaagaatc cgtgcgccgt caccgcctcc
     1861 acccccagag agccgacgat cagcggcagc acgaacgagg ccgccgagta cccgtagtcg
     1921 atggcgatgc gcgccccacg gtcgcgtatg gcggcgatgt cgagcgtgtc gagcagatcc
     1981 tgcgcgtagc tttcgcgcac gcgggtcgga tacgtcgtct cgccgatctc ctcgaaagct
     2041 gcgcggcgaa gctcgcgccg cgtgtagtga cgctcgacct ctcgctgcag agaggacgag
     2101 atctgtgtgc cagggaactc gaacacgcga atctggacga gctccgggtc gaccgcactc
     2161 cgggccacgt gaacgcccgc ggccagccct tgcgtcttca ggacatgacg cgtgatcgcc
     2221 gacggcgata tccggaggtc cgcgacgtcg acgcccgccg acgtcagccc gacgatcatg
     2281 gcgcgctgga tcatcctgca ggcgtcgggg gactcgcggc tcgcgaccac gcgcgcgccg
     2341 ctgtcgagcg cacttcccag cgctcccgcc aggcgaaccg cgtcctccgg ggtgaggtcg
     2401 acgttgacga ggccggtgac gccgtcgacc ccgaagaccc gctgggacac ccgcgtctcc
     2461 cagatgaggc tctcgtggat ctgcgctccg gtctcgacct ccttgaacgg gtagacgcgg
     2521 actccgggca tgatgctcgc ctcgggtccg atcgtcacct cgtcgccgat tgccacgccc
     2581 tcgtgcactc gcacgtggtt ccggatgtcg cacgagcgac ccacgatcgc cccttccacg
     2641 agcgcgctgc ggccgatgta cgtcgaggcg tcgatgatcg atcgcgcgac ccggccgcgc
     2701 tcacgcagcg tgacgccctg gccgagaacg gtgtaggcac ccactgacgc gtcgggggca
     2761 atgcgacagt tcgcgccgac gtacgccggc ccctccaggt gctcgacgtc gtcgatgttg
     2821 accccttcgc cgatccagac gttcccgcgc aggcggattc ccttgatgga gagccgcacg
     2881 tcgccgtcga gtgcgtcgaa gttcgcctgt cgatactggt cgaggttgcc gatgtcctgc
     2941 cagtagccgt ccatcacgtg cccgtagatc ggacggccca tgtcgagcag gagcgggaat
     3001 agctccttcg agaagtcatg cggctgaccg gtcgggatgt ggcgaagcac ctccggctcg
     3061 atgacgtaga taccggtgtt gatcgtgtcc gagaagacct ggccccagga cggcttctcg
     3121 aggaagcgct cgactcgacc ttcgtcgtcg gtcaccacga tgccgaactc gagcgggttc
     3181 tcgacggact tgagaccgat ggtgacggcg gcgccggtgc ggcgatgcgc cgctaccagc
     3241 tcggtgagat cgacgtcaca cagcgcgtcg cccgagatga cgaggaacgt gtcgtccagc
     3301 cgatggctgg cgagcgcgac agaaccggct gttccgagcg gctgctcctc gatcgagtag
     3361 tcgatcgtga gccccatcgc ctccccgtcg ccgaagtggc cccggatcgc ctgcggcatg
     3421 aaggcgagcg tgacgacgat ctcgcggaag ccgtgcgtgc ggagcagctc gaggatgtgc
     3481 tctatgcacg gcttccccac tatcggaacc atgggcttcg gctgattcga cgtcaggggc
     3541 cgcagccgcg tcccttcgcc cccggccatc acgaccgctt tcactccctc cccacctccc
     3601 ggcgcgcctt cagcatgtac tgcaccgcgg cggcgaccgc gagcgcgacg ccgatccaga
     3661 acagccagag cggccacgcg gttcccttct cggtcacgat cacacagagc agcgagaagt
     3721 agagaaccca cgttgcgagc ttgccgagga agctcacctc gaagtcgtac ccgcgcggga
     3781 cgacgaagcg atatccgagt accaacagaa gatcgcgccc cagtacgatt accgccgcgg
     3841 gccacggcag acgcccgtcg atccagagta agactgctgc cactccgatc atcaagcggt
     3901 cggcgagcgg gtccgcgacc ttcccgaact ccgattcgac cttccaccgg cgggcgagcc
     3961 agccgtcgag ctggtcggtc gcggcggcaa cggcgaagac gagtcccgcc caccaggcgg
     4021 cgccgtcgcc cgcgttgatg tacagcacga tgaagatcgg tatcgcggcg aagcggagaa
     4081 tcgtcagcgc gttcggaagc tggcgcctcg gtgcggaaat cgtgctcgtg ctcactccgg
     4141 ccatcgtagt ggtgctagcg tcgtttcgtt ctttccgatg catcgctgga cgtctcacac
     4201 gggcgaggaa caactcgaga tcgaggcgga caccccggcc ggcgttttcg ccgaggccgt
     4261 cgatgccttc gggcggtcgg tggagctcgc gaaggacggt gcgccggctg cccacgccgt
     4321 ccacctcgag gggcgggagc ccgctgcgct cctcgttcag ctgctcaacg agctcgtctt
     4381 cctcgccgac accgagggct tcgtgcccga ccgatcggag ctcgtgctcg aggacgctgt
     4441 cctacgcggg cgaatcctcg gccgatcgac gaccgtgcgg ccgctcgtga aagccgcaac
     4501 gtacggcggt ctcgcgttcg aggagttcga tggcttctgg cgcgctcggg tcgtgctcga
     4561 tgtctgatct gcgccagatc gacgagacgc tctgggagat ccccgccgag tcacgcgcgg
     4621 acatgcgcgt gccggcgcgg gtcttcgccg atcgcgacat cgttgccgcg atcgagggcg
     4681 acctgtcgct cgagcagctg cagaacgtga caacactgcc ggggatcgtg ggcgcggcga
     4741 tcgcgatgcc cgacatccac cagggctacg gtttccccgt cggcggcgta gcggcgacgg
     4801 agcttcccga cggcgtcgtg tccccgggag gcgtcggcta cgacatcaac tgcggcgttc
     4861 ggctcctctc cctcccgctc tcggagagag acctgggtgg gcgacgcgaa gctttggtgc
     4921 acgacatctc gcgctccgtg ccggcgggag cgggcaagca cggagtgctg gagctggacg
     4981 acctgacgct ggactcggtt ctgagtgagg ggccgcgcgc attgccgaat gtccgcccgc
     5041 gggatatcga gcacaccgaa tcgggcgggc gcctcgaagg cgccgatccg acagccgtgt
     5101 cgaagcgggc gcatgcccgc gggcgagcgc aactgggaac catgggatcc ggcaaccact
     5161 tcgtcgagct ccagatgatc gagtcggttc tcgacgaccg agtcgcgtcg gccttcggcc
     5221 tcgtcgcggg gcaggtgacg gtgctcatcc actccggctc ccgtgggctc gggcatcagg
     5281 tctgcaccga ctacgtgcga cagatggacg acgcccagga acggtacggg ttcgccctgc
     5341 ccgaccgtca gctcgcgtgc gcccccgcct cgtccccgga aggagcggcc tacctcgccg
     5401 ctatggcggc tgctgcgaac ttcgcgtggg ccaatagaca ggtcatcgcc gacagtgtcc
     5461 gcgcggcgat tcgaagtgtt ctcggcgacg atgcggcgga cgccacgaac caggtgtacg
     5521 acgtcgccca caacgtcgcc aagagagagc gacatctcgg caaggacgtg ctggttcatc
     5581 gcaagggcgc gactcgtgcc ttcccgcccg gctcgaccga gcttcccggc gagtatgcga
     5641 cggtcggtca gccggtcttc atccccggca gcatggggac ggcgagcttc gtcctggtcg
     5701 gcgagcccgc cgcgatggag aagtccttcg gcaccgtgtg tcacggcgcc gggcgtgttc
     5761 tctcgcggac ggccgccagg aaacggatca gtggacacga cctccggcgg gaactggagg
     5821 ggcgcggcat cgtagtgagg agcccgtcgg cgcgcgggct cgccgaggag gcgccgttcg
     5881 cctacaagga cgtcgacgcc gttgtgcgcg tcgttgagca agccggtctc gcgcggcgcg
     5941 tcgcccgcct cgtgccgctc ggtgtggtca agggctgacg aacgccggca gcggctagat
     6001 tcaggctatg gatgtcactg agacaacgtt cgaggctgcg gtgatcgagc ggtcgcgcga
     6061 gcttcccgtg atcgtcgact tctgggccga gtggtgtggc ccctgccatg cgctgacacc
     6121 tgtgctcgaa agtgcggtca gcgagcgaag cggtgccgtc gagctggtga aggtcgacgt
     6181 cg
//
LOCUS       DBNGNK010000348         8310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_990315,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000348 DBNGNK010000000
VERSION     DBNGNK010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8310
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_990315"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="AC150V_10310"
     CDS             <1..555
                     /locus_tag="AC150V_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763351.1"
                     /translation="GAGSSASRAALIGAAAGSRAGTVTASGDGSTTSSCGDARSSTDG
                     GAGSDGAARTGGGGGGLDSGTAGIVVLAGGAGDTGSGEGASTGAGGVSGAACGEESVG
                     AGVGFGLTARVACWPCARAGDSSGCATPPGRPRNGASTGADDLTGGWGTGVSTAAATG
                     SWTRAGAAFGAAGGAVFAAGVAGG"
     gene            1021..1362
                     /locus_tag="AC150V_10315"
     CDS             1021..1362
                     /locus_tag="AC150V_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763352.1"
                     /translation="MAASASRHAIARDEARRLTAVPGVVPLSSYPLSATQGTDVGRAA
                     QAEPTAGALASAGGLSAAGGGRVELPAAVHCAASGMPEHVYQALVEVEYLEHGLYQPK
                     RASGLALGPHL"
     gene            1376..2128
                     /locus_tag="AC150V_10320"
     CDS             1376..2128
                     /locus_tag="AC150V_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR3763353.1"
                     /translation="MAMTGPGGTGAAAFHLRAIDRSRLYASIVEQILDGIESGAFRPG
                     TALPAERSLAADLGVSRGSVREAIRVLEHAGILEVRTGSGTFVVDPGPPKVALLRAQA
                     ALTGEHSPLDVMAARRAVEPACAELAAQRRQERDLELIRGALDAQARLVQEGEDAAAA
                     DLDFHLAVATATHNPVLLSLLERVAEIMRRPPWTELKHASRGGDRTGAERDVREHRAI
                     LAAVEERNTAEATVAMLVHLASVERDLLEHVD"
     gene            2212..3129
                     /locus_tag="AC150V_10325"
     CDS             2212..3129
                     /locus_tag="AC150V_10325"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR3763354.1"
                     /translation="MGPTDLRGAIPAIPTPFTADGREVDDDALRRIVRHVVDGGVHGI
                     MTTGGTGEFPHLSREERRRVTEVAASEASGAAPIYAGTAACATWEAIELMEEAAGAGA
                     DAAILTPPFYFGSPDEMLFQHFADVARAGVLPVVVYNNPLYTGNDLGPALIAALMELD
                     NVIGLKQSNDDLGQLVESLRLAEGSGASLCTGVDSQFYAALCVGAKGIFSTAASIVPA
                     QVVRLYDLVLAGDHDAAAEQHRLLQPLNRFLEYDPGYVSPTKEALELMGMSCGPVRRP
                     LPDFPEAERPALREALVALGALDASGVPA"
     gene            3126..4061
                     /locus_tag="AC150V_10330"
     CDS             3126..4061
                     /locus_tag="AC150V_10330"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3763355.1"
                     /translation="MRLVTFEHDGRISLGAVSGESVVDLRFPADMVAFVSGGDQSLAW
                     AESKLASPGDDVYPLGDVRLLAPLVPPVILNSGQNYWDHRDEKPEVDQQVPEFFLKTP
                     LAVIGPDEPVLYDRIVTNKLDYEVELGVVIGKPGRHIPVDRALEHVFGYTIANDITAR
                     DRQAVPHPEGGWEYALGPGKNFDTSAPLGPWIVTRDEIPDPQNLELRTYVNEEIRQSN
                     STAKMIWSVAEIVAFFSHFYTLQPGVLLETGTPGGTAWATDPEIGGKPFDRDDVVRKG
                     YLDVGDVVRVEIEGIGSLSNPIAAAPTPQSEGGAL"
     gene            4058..5446
                     /locus_tag="AC150V_10335"
     CDS             4058..5446
                     /locus_tag="AC150V_10335"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR3763356.1"
                     /translation="MTLLHPDAPTLFINCHLIDGHGGEPIENGAVKVEGNLITAVGRT
                     DDFGDQNGDLRIVDLAGKTLMPGLIEGHFHISFWGVRELPDLDLKLPAERTTVYAVKN
                     AELALRCGYTGVASAGALHRIDVTIRDMVKEGVIPGPRMAASGRDVCATSGMLDWNPS
                     FWKLGMDGLAIFADGVEEVRKAVRQNIHEGCDVIKLYVTGEGLLRPGVPPEETMYSFE
                     EIKVAVDEAHKRNRQIAAHVRGNDGVKLCVEAGVDVLEHATYADDEAVAMIAEKKDEM
                     FVVPGLGYHWGILEKGVACGIPPDVLAATEYQEEWELGCQAMDKLRRAGVRVIPGGDY
                     GFVWCPHGEYAKDIELFVTDMGFSPMEAIVASTKHGAELLRLEHECGTIEEGKCADIL
                     VVDGDPLQDIAILQERSKLALVMANGKIMVNTLGLAQELTHIPDFKMDIEHIQNANAV
                     APTMPTAAEVQH"
     gene            5529..6323
                     /locus_tag="AC150V_10340"
     CDS             5529..6323
                     /locus_tag="AC150V_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3763357.1"
                     /translation="MTDVEINGCRLSVQEHGSGAPVVLVHGLGGTAGEIWKHQLPELA
                     AAFRVVAFDLRGSGASEVTPGPYTIELLVDDLRALVEALGLGRIGLLGHSMGGSLVLS
                     YAARYPSDVTALVGVGAPASLPDAAREGLAARAEAVEAEGMAAVAETVATNGVSPTVR
                     ERRPDEFRDLIGMLERNDPNGYAAQCRALVGLDLTGQLAAITAPVLLVSGDKDGVSPP
                     TAPEATADLVTGGRFTLVDDCGHNLTVEQPEALAAAAWPFLREALA"
     gene            6436..6843
                     /locus_tag="AC150V_10345"
     CDS             6436..6843
                     /locus_tag="AC150V_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763358.1"
                     /translation="MGSGRLKRWAPASGTVYVLLFVVAFIVSGSDSGTTDEEIVSSYA
                     DSGNRISEIVAFFLVTVGLLFFVWFASTLRDRLKSVESEPKSLSALAFGAGLVSAALM
                     IGAMALFVATSFTVVDADEFVVDPNTARVLENA"
     gene            complement(7057..7130)
                     /locus_tag="AC150V_10350"
     tRNA            complement(7057..7130)
                     /locus_tag="AC150V_10350"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7094..7096),aa:Ile,seq:cat)
     gene            7239..7691
                     /locus_tag="AC150V_10355"
     CDS             7239..7691
                     /locus_tag="AC150V_10355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3763359.1"
                     /translation="MKTYQHEVQLRTAGGLTVTDITADVAEAVEASGIQNGICCVYSP
                     HTTCSVRVNEWESGFLEDFAVLLRRLVPAEHYYAHDDWDRRTENICPEDMEVGNGHAH
                     CMSMLLGPAGESIPVREGELCLGQWQRILFLELDRERDRRWLVQVVGV"
     gene            complement(7709..>8310)
                     /locus_tag="AC150V_10360"
     CDS             complement(7709..>8310)
                     /locus_tag="AC150V_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3763360.1"
                     /translation="EVDVASEDAVRTLMASAAEALGGLDVLYNNAGISPPEDASILDT
                     PVEAWERVQDVNAKGVFLCCKHGIPYLLDRGGGSVINVASFVAILGAATSQISYTASK
                     GAVLALSRELAVEFARQGVRVNALCPGPVETPLLRQIYGDDPAAFERRRVHLPMGRLA
                     RPREIVDVALFLASDEASYVTGAVVLADGGLTAAYVTPE"
BASE COUNT         1314 a         2843 c         2927 g         1226 t
ORIGIN      
        1 ggagccggtt cgtccgcgtc acgcgcagcg ctgatcggag ccgccgccgg ctcacgcgcc
       61 ggcacggtca cggcctccgg cgacggaagc acgacgtcgt cctgcggcga cgcgcgcagt
      121 agcacggacg gcggcgcggg cagcgacgga gccgcccgga caggcggggg cgggggcggg
      181 ctcgactcgg ggactgccgg gatcgtcgtg ctggcgggcg gtgcgggtga caccggctca
      241 ggggagggag ccagcacggg cgccggcggc gtttcgggcg cagcgtgcgg ggaggaaagc
      301 gtgggcgcgg gcgtcggctt cggtttgacc gcccgggtcg cctgctggcc ctgcgcccgt
      361 gcgggcgact cctcgggctg cgcgaccccg ccgggaaggc cgcgcaacgg ggcgagcacg
      421 ggcgcggacg acttgaccgg cggctgggga accggcgtct cgacggcggc ggcgacgggc
      481 tcgtggacga gggccggcgc agccttcggc gccgcggggg gagccgtctt cgccgccggt
      541 gtcgcgggcg gctgagtcgt ggcggcggcc ggagccggag tcgggcacga ggtcgcgggg
      601 acaccaccgt caccggctgc cgggaccgcg gcacaggcaa ccgggccccc ttcggtcggc
      661 ccggccagca ctggaacggg ctcgacgggc accggttcga cgggggtcgc cgccggatct
      721 aggggcgcgg gatcgaccgg ggccggatcg ttcggcgtcg caggcgggtc gaccggagct
      781 gcgactggat ccaccggcac gggctcagag ggggccggct ctgcaggtgc gggctctgca
      841 ggtgcgggct ctgcagaagc gggttcgacc ggcgccggct cgacgggcgg gggggccggc
      901 tcggaaggcg ggacggtcac accacctgtg cttcctgagt ccgtaggtgt cggctccgcc
      961 ggaggggtcg gggtcgactg gccgagcgcg accgcgggaa gaacgagcga agccccgacg
     1021 atggccgcga gcgccagcag gcatgcgatc gcgcgcgacg aagcgcggcg tctgacggct
     1081 gtgcccggag tggtccccct cagttcctac cccctttccg ccacacaggg tacggacgtc
     1141 ggccgggctg cgcaagccga accgacagca ggggcgctcg cgtcggccgg cgggctttcc
     1201 gccgccggcg gcggacgggt cgagctgccc gctgcggtgc attgcgcggc ctccggtatg
     1261 cccgagcacg tatatcaagc tctggtcgag gttgagtatc tcgagcacgg cttgtaccag
     1321 cccaagcgcg cgtccgggtt ggcgctcgga ccgcatctgt agcaggatct acggaatggc
     1381 gatgaccggc ccgggcggca cgggagcggc ggcattccac ctgcgcgcga tcgacaggtc
     1441 gcggctctac gcctcgatcg tcgagcagat cctcgatggg atcgagtcgg gagcatttcg
     1501 cccgggcacg gcgctcccgg ccgagcgatc cctcgccgcc gatctcggcg tcagccgagg
     1561 ctcggtgcgc gaggcgatcc gcgtgctcga gcacgcaggc atcctcgaag tacgcaccgg
     1621 aagcggcacg ttcgtcgtcg accccggccc gccgaaagtg gcgctgctac gggcgcaggc
     1681 cgccctcacc ggcgagcaca gcccgctcga cgtgatggct gcacggcggg ccgtcgagcc
     1741 ggcgtgcgcg gagctcgccg cgcagcgccg acaggaacgc gatctggagc tgatccgggg
     1801 cgcgctcgac gcacaggccc gcttggtgca ggaaggcgag gacgcggccg cggccgatct
     1861 cgacttccat ctcgcggtcg ccaccgcgac tcacaacccg gtgctgctgt cgctgctcga
     1921 acgggtcgcc gagatcatgc gccggccgcc ctggaccgag ctcaagcatg cctcgagagg
     1981 cggcgaccgg accggagcag agcgcgacgt gcgcgagcac cgcgcaatcc tggcggcggt
     2041 cgaggagcgg aacaccgcgg aggcgaccgt ggcgatgctg gttcacctcg cgtcagtcga
     2101 gcgtgacttg ctggagcacg ttgactaggc tctcatcggt tcggtggtcg gaccaatctc
     2161 ggcacgagac cggctacaac acgctgaccc gtcacgggag aaggaggcca agtgggaccg
     2221 actgacctgc ggggtgcgat ccccgccatt ccgaccccgt tcaccgcgga cggcagagag
     2281 gtcgacgacg acgcactccg acgcatcgtc cgccacgtcg tcgacggagg ggtgcacgga
     2341 atcatgacca cgggcgggac gggcgagttc ccgcacctct cgcgcgagga gcgccgacgc
     2401 gtcacagaag tcgccgcgtc cgaggcgtcc ggagccgcgc cgatctacgc agggaccgcc
     2461 gcctgcgcga cgtgggaggc gatcgagctg atggaggagg cggcgggcgc cggcgccgac
     2521 gcggccattc tcacaccgcc gttctacttc gggagtcccg acgagatgct cttccagcac
     2581 ttcgcagacg tcgcccgggc gggggtgctg ccggtcgtcg tctacaacaa ccccctctac
     2641 acgggtaacg acctcggccc ggcgctgatc gcggctctga tggagctcga caacgtgatc
     2701 ggtctcaagc agtcgaacga cgacctcggc cagctggtcg agtcgctcag actcgcggag
     2761 ggcagcggcg cgtcgctctg caccggcgtg gacagccagt tctacgcagc actctgcgtc
     2821 ggcgcgaagg gaatcttctc gaccgccgcc tcgatcgtcc cggcccaggt cgtacggctc
     2881 tacgacctcg tgctggcagg cgaccacgac gcggccgcgg agcagcacag gctgctgcag
     2941 ccgctcaatc gcttcctcga gtacgacccg ggctacgtct cgccgacgaa ggaagcgctg
     3001 gagctgatgg gcatgtcgtg cggccccgtc cgccggccgc tccccgactt cccggaggca
     3061 gagcgtcccg ccctgcggga ggccctcgtc gcgctcggcg ccctggacgc aagcggggtt
     3121 cccgcgtgag gctcgtcacc ttcgagcacg acggccggat ctcactcggc gcggtctccg
     3181 gcgagagcgt cgtcgatctc cgtttcccgg ccgacatggt ggcgtttgtc tcgggcggcg
     3241 atcagtcgct cgcctgggcg gagagcaagc tcgcgagccc gggcgacgac gtctacccgc
     3301 tcggtgacgt ccggctgctg gcgccgcttg ttcccccggt cattctcaac tcgggccaga
     3361 actactggga tcatcgcgac gagaagccgg aggtcgacca gcaggtgccc gagttcttcc
     3421 tcaagacgcc gctggcggtg atcggcccgg acgagccggt gctctacgac cggatcgtca
     3481 ccaacaagct cgactacgag gtggagctcg gcgtcgtcat cggcaagccg gggcgccaca
     3541 tccccgtcga tcgggcactc gagcacgtct tcggctacac gatcgcgaac gacatcacgg
     3601 cccgcgaccg ccaggccgtt ccccatccgg agggcggctg ggagtacgcg ctcggacccg
     3661 gcaagaactt cgacacctcg gcacccctcg gcccgtggat cgtcacgcgg gacgagatcc
     3721 cggatccgca gaacctcgag ctccgcacct acgtcaatga ggagattcgt cagagcaact
     3781 cgactgcgaa gatgatctgg agcgtcgcgg agatcgtggc gttcttctcc cacttctata
     3841 ccttgcagcc cggcgtcctg ctcgagacgg gcacaccggg cggcaccgcc tgggccaccg
     3901 accccgagat cggtggcaag ccgttcgacc gcgacgacgt cgtccgcaag ggctatctgg
     3961 acgtcggtga cgtcgtccgc gtggaaatcg aagggatcgg ctcactgtcg aacccgattg
     4021 ccgcggcgcc gacgccgcaa tccgaaggag gcgcgctgtg acactgctcc accccgacgc
     4081 accaacactc ttcatcaact gccatctgat cgacggccac ggcggagagc cgatcgagaa
     4141 cggcgcggtc aaggtcgaag gcaacctgat caccgccgtc ggccgcaccg acgacttcgg
     4201 cgaccagaac ggcgatctcc gcatcgtcga cctcgccggc aagacgctca tgcctggact
     4261 gatcgaaggg cacttccaca tctcgttctg gggtgtgcgc gagctgccag acctcgacct
     4321 caagctgcct gccgagcgca cgacggtcta cgcggtgaag aacgccgagc tcgccctgcg
     4381 ctgcggctac acgggcgtgg cgagcgcagg cgcgctgcac cgaatcgacg tgacgatccg
     4441 tgacatggtg aaggagggcg tcatccccgg tcctcgcatg gccgcttccg ggcgtgacgt
     4501 ctgcgccacg tcggggatgc tcgactggaa cccgtccttc tggaagctag gcatggacgg
     4561 cttggccatc ttcgcggacg gcgtcgaaga ggtgcgcaag gcggttcgcc agaacatcca
     4621 cgagggctgt gacgtgatca agctctacgt cacgggcgag ggcctgctcc gcccgggcgt
     4681 gccgccggaa gagacgatgt actccttcga ggagatcaaa gtcgccgtcg acgaggcgca
     4741 caagcgcaac cgtcagatcg ccgctcacgt gcggggcaac gacggcgtca agctctgcgt
     4801 cgaagcgggc gtcgacgtgc tcgagcacgc aacatatgcc gacgacgagg ccgtcgcgat
     4861 gatcgcggaa aagaaggacg agatgttcgt cgtccccggc cttggctacc actgggggat
     4921 cctcgagaag ggtgtcgcct gcggcatccc gcccgacgtg ctcgccgcaa ccgagtacca
     4981 ggaggagtgg gagctcggct gccaggcgat ggacaagctc cgccgggccg gagtgcgggt
     5041 catccccggc ggcgactacg gcttcgtctg gtgcccgcac ggcgagtacg cgaaggacat
     5101 cgagctcttc gtgaccgaca tgggcttctc gccgatggag gcgatcgtcg cctcgaccaa
     5161 gcacggcgcc gaactgctcc gcctcgagca cgagtgcggc actatcgagg agggcaagtg
     5221 cgccgatatc ctcgtggtcg acggagaccc gctccaggac attgcgatcc tgcaggagcg
     5281 ctcgaagctg gcgctggtga tggccaacgg gaagatcatg gtgaacacgc tcgggctcgc
     5341 ccaggagctg acccacatcc ccgacttcaa gatggacatc gagcacatcc agaacgcgaa
     5401 cgccgtggcg cccacgatgc cgacggcggc cgaagtccag cactgacgac cgcggccggt
     5461 gggaggccgg gcggtgtcgc ccggccgccc accggccggc tcagctgtcg atcgaaggga
     5521 ggacgagagt gaccgacgtg gagatcaacg gctgccggct gtcggtgcag gagcacggct
     5581 cgggcgcgcc ggtcgtgctc gtgcacgggc tcggcgggac cgcgggtgag atctggaagc
     5641 accagctccc cgaattggcc gccgctttcc gcgtcgtcgc gttcgacctg cgcgggtccg
     5701 gcgccagcga ggtgacaccc ggcccgtaca cgatcgagct gctcgtcgac gatctccgcg
     5761 ccctcgtgga agctctcggc ctcggccgga tcgggctcct cggccactcc atgggtggct
     5821 cgctcgtcct ctcctacgcg gctcgatatc ccagcgacgt gaccgctctc gtcggcgtcg
     5881 gagctccggc gtcgctccca gacgcggccc gcgagggcct ggccgcgcgc gccgaggccg
     5941 tcgaagccga gggtatggca gcggtcgccg agacggtcgc cacgaacggc gtctccccca
     6001 ccgtccgcga gcggcgcccg gacgagttcc gcgacctgat cgggatgctg gagcgcaacg
     6061 atccgaacgg ctacgcggcc cagtgccgcg cgctcgtcgg actcgacctc accgggcaac
     6121 ttgcggcgat caccgccccg gtgctgctcg tctcgggcga caaggacggc gtgtcgccgc
     6181 cgacggcacc cgaggcgacc gccgatctcg tcaccggcgg ccgtttcacg ctcgtggacg
     6241 actgcgggca caacctcacg gtggagcagc ccgaagcgct ggccgccgcg gcgtggccgt
     6301 tcctgcgcga ggcgctcgcg taggaaacgc cggcccctcg cccatatcgc cgcgagtacg
     6361 accgccttga cttgtttcta ttgatacagc attcgtgcgc tgctcgtggt tcgggttgtg
     6421 aggaggtgcg gaccgatggg cagcggtcgc ttgaagcgtt gggctccggc tagtgggacc
     6481 gtctacgtgc tgctcttcgt ggtcgcgttc atcgtcagcg gcagcgacag cggcaccacg
     6541 gacgaggaga tcgtctcctc ctacgcggac agcggaaatc gcatcagcga gatcgtcgct
     6601 ttcttcctcg tgacggttgg actcctcttc ttcgtttggt tcgcgagcac gcttcgcgat
     6661 cgactcaagt cggtcgagtc cgagccaaag agcctctccg cactcgcttt cggagccggt
     6721 ttggtgtctg ccgccttgat gatcggcgcg atggcgctct tcgtcgccac gtcgttcacg
     6781 gtcgtggacg ccgacgagtt cgtggtcgat cccaacacgg ctcgagtcct cgaaaacgct
     6841 tgatatctgc tcttcccccc tcgacgatgg tcgccacccc atcttcctgt tgtgggcttg
     6901 agttctggcc gttgcgggcg tcctgatcgt gcgggcgccg gcggccgagg cgggggctgg
     6961 cccgtccgcg acccgctaaa cgcagtccgt cgccacagcg acccgagctg caataggcat
     7021 cgatgtgaaa gccctgcgca agcagggctt tcactgtggg cccgcctgga ctcgaaccag
     7081 ggaccaacgg attatgagtc cgccgctcta accacctgag ctacgggccc gtccggctga
     7141 gactagctcc ttccacccca cgaggctccg cgcggcctga caaaatgcac gggccggggg
     7201 ccgagcgccc gaagcgacga gggagaaacg agggcagggt gaagacctac cagcacgagg
     7261 tgcagctgag gactgcaggc ggactcaccg tgaccgacat caccgccgac gtcgccgagg
     7321 ccgtcgaggc gagcggcatt cagaacggga tctgctgcgt ctactcgccc cacacaacgt
     7381 gctccgtccg tgtcaacgaa tgggagtcgg gcttcctcga ggatttcgcc gtcctgctcc
     7441 gccggctcgt tccggccgag cactactacg cgcacgacga ctgggatcgc cgaacggaga
     7501 acatctgccc cgaggatatg gaggtcggga acggtcatgc gcactgcatg tcgatgctgc
     7561 tcggaccggc cggcgagtcg attcccgtcc gcgagggaga gctctgcctc ggtcagtggc
     7621 aacggatctt gttcctcgag ctcgaccgcg agcgcgatcg gcgctggctc gtccaggtcg
     7681 tcggcgtctg agggcgaaga gagccgggct actcaggcgt gacgtaggcg gcggtcagcc
     7741 cgccgtcggc aagcaccacc gcgccggtga cgtaagacgc ctcgtcgctg gccaagaaga
     7801 gcgcaacgtc gacgatctcg cgcggccgtg cgagccgtcc catcggcagg tggacgcgcc
     7861 gccgctcgaa ggctgccggg tcgtcaccgt agatctgccg caagagtggc gtctccaccg
     7921 ggccggggca cagcgcgttg acccgcaccc cttgccgcgc gaactcaacc gcgagctctc
     7981 gactgagtgc gagcacggcc cccttcgagg ccgtgtacga gatctgcgac gtcgccgcac
     8041 cgaggatcgc gacgaaggac gcgacattga tcaccgagcc gccgccgcgg tcgagcagat
     8101 acgggatccc gtgcttgcag cagagaaaga ctcctttcgc attgacgtcc tgaacccgct
     8161 cccaggcctc gaccggcgtg tcgaggatcg aggcgtcctc gggcggggag atgccggcgt
     8221 tgttgtagag gacgtcgaga ccgcctagcg cctcggcggc cgacgccatc agggtgcgga
     8281 cagcatcctc gctcgcgacg tcgacctcga
//
LOCUS       DBNGNK010000349         5560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_489675,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000349 DBNGNK010000000
VERSION     DBNGNK010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_489675"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1087)
                     /locus_tag="AC150V_10365"
     CDS             complement(35..1087)
                     /locus_tag="AC150V_10365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR3763361.1"
                     /translation="MSDAQLPDEALALLVRGEREGALQLSELEELVERMELDDDQVGA
                     LYAEAETRGIALADDVGRASSSRTGYANGDLAGATTDSLQLFLNEIARYPLLTAPQEV
                     ELAKRIERGDQEAKDRMINSNLRLVVSIAKRYQGHGLSLLDLIQEGIIGLIRAVEKFD
                     WRRGYKFSTYATWWIRQAVQRGVANKARTIRIPVHVADLERKLARAERELVNRLGREP
                     TETELADASGIGVEEIRDVREVFRSVTSLDKPVGEGDGAALGDLIADSTAGPEVELDL
                     LLSETALVAALGRLSTREREVLTLRYGLSGGEPQSLGKIGEQIGLTRERVRQIEQEAL
                     SRLALMRELEAMSNAA"
     gene            complement(1172..1537)
                     /locus_tag="AC150V_10370"
     CDS             complement(1172..1537)
                     /locus_tag="AC150V_10370"
                     /inference="COORDINATES: protein motif:HMM:NF037170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3763362.1"
                     /translation="MANPVVHFEIIGSDDGALGGFYANLFDWKVNPVDGMDGYRMVDT
                     GHEGSIGGGIGASETGQSHVTFYVQVDDPQAYLDKAESLGDKTIVPVTEVPDIVTFAL
                     LADPEGHVVGVVKDDNGQQ"
     gene            complement(1635..>5560)
                     /locus_tag="AC150V_10375"
     CDS             complement(1635..>5560)
                     /locus_tag="AC150V_10375"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIR3763363.1"
                     /translation="LLLVSKTNRNATVHRRAKMDYIGVKSVSPDGAVLGELRMVGLFT
                     RKAYSEPAGRIPLLRLKLQRVLAAEDFVPGSHDHKAAVELIENFPLDELLSVDQQTLQ
                     RTVAELISLQEQQKVRLFAHRDLFDRSVSVLVALPRDRFNAPLRKRLQALFRERFHGS
                     SVDYHLSLGQSDQALIHFRIHVGHGAIPDVDYAALEHEVIALTRTWDDRLLERLVALH
                     GEEHGGELATKWSERLPDYYKSATDVYRAVIDIGHLEQLGGQQPFVVALRNERGQRPE
                     TRVHLYKTGGRANLSELMPGLEALGLVVVEEVPIAVEGDADIHIHNFGVVGPDGEAVD
                     VPALGELVANALGAVWRREAESDSLDRLIVLAGLTWPQVAVLRALRTYARRVSASFTL
                     EYENAAFAANPEIAADLIRFFELRFDPSRPRDEDAEAELRERIVEKLDSVPSLDHDRI
                     LRGYLQVIEAIVRTNAFRPGRRYLSFKIRSEQVPDVPKPYPLYEIFVYSPEMEGIHLR
                     FRQVARGGVRWSDRQEDYRTEILDLMKTQTTKNAVIVPSGSKGGFVLKREVPPQETRD
                     EVRRQYETFIRGLLDLTDSLADGEVVHPPDVRILDEADPYLVVAADRGTASFSDIANA
                     ISQEYRFWLDDAFASGGSTGYDHKKLGITARGTWESVKRHFRELGRDVMTDPIRVAGI
                     GDMSGDVFGNGMLYTEQIRLVAAFDHRHVFLDPTPDPITSFAERKRLFELGRSTWDDY
                     DRDLISRGGGIWPRSAKSISISEEAAEALGTEPGSVTPTELISTILRAPVDLLWNGGV
                     GTFVRATDETDAEVGDRANDAMRVTGRDVRALVVGEGGNLGFTQRGRIEYANAGGRIN
                     TDFIDNSGGVDCSDHEVNLKILLGLAMERGDLTRKQRNELLAESAEDVTRHVLYHNYL
                     QAQILSQKVAISPKRIREFEDVMRSLEDQGLLDRPIEYLPSSEEMAERERAGLGMARP
                     ELAVLLAYAKRSLKRWVLPSSLPDDPYLERDLRAYFPPRVAERFDDLIKEHPLRRDLI
                     ATIVSNDVVDSQGITFVWRFVAETGAEPPDVIRAYRIAREVTAAETRWADIEALDGVI
                     DPVVQNELMMGVDWLVEMTARWFVMNAPGADIGETVETMAQGFNELDFVLPELIPEAS
                     KVEREREAAGLVSRGAPETLARRNEYQPALTHGPDIVTVARGSDRSVTDVARAFFEAG
                     QSLHLDWLEDQVVALQPTSRWERVAFNTVVDDLLLVRREAVQKAIAACDDDTQTAEQA
                     LESYLASRPEAVGRLDRLVDQLDTEGINDLAVITVAVRQVRGAVA"
BASE COUNT          825 a         1889 c         1890 g          956 t
ORIGIN      
        1 ctcctccgcg gcgcctgagc gcccgcgggc gggctcaggc ggcgttgctc atcgcctcga
       61 gctcccgcat cagcgccagc cgggagagtg cctcctgctc gatctgacgc acccgctcgc
      121 gggtgagacc gatctgctcg ccgatcttgc cgagcgactg cggctcgccg ccgctcagcc
      181 cgtagcgcag agtgagcacc tcccgctcgc gcgtgctgag gcggccgagg gcggcgacga
      241 gggccgtttc gctgagcagg aggtcgagct ccacctccgg gccggccgtg ctgtcggcga
      301 tgaggtcacc gagcgcggca ccgtcgccct ccccgacggg cttgtccagg ctcgtcaccg
      361 atcggaagac ctctcgcacg tcgcggatct cttcgactcc gatcccggac gcgtcggcca
      421 gctccgtttc ggtgggctcg cggccgagcc ggttcaccag ctcccgctcg gcgcgcgcaa
      481 gcttccgctc caggtcggcg acgtgcaccg gtatgcggat cgtccgggcc ttgttcgcga
      541 cgccgcgttg cacggcctgc cggatccacc acgtggcgta cgtcgagaac ttgtagccgc
      601 gtcgccagtc gaacttctcg acagccctga tcaacccgat gatgccctcc tggatcagat
      661 cgagcagcga caagccgtgg ccctggtagc gcttcgcgat cgagacgacg agccggaggt
      721 tcgagttgat catccggtcc ttggcctcct ggtcgccgcg ctcgatgcgt ttcgccagct
      781 ccacctcctg cggtgcggtg agcagggggt accgcgcgat ctcgttcagg aagagctgca
      841 ggctgtcggt cgtggcgccg gcgagatcgc cattcgcgta gccggtgcgg gaactggaag
      901 cgcgtcccac gtcgtcggcg agagcgattc cgcgcgtctc cgcttccgcg tagagcgcgc
      961 cgacctgatc gtcgtcgagc tccatccgct ccacgagctc ctcgagctcg gagagctgta
     1021 gtgcgccctc ccgctcgccg cgaacgagaa gcgcgagagc ctcgtcgggt agctgcgcat
     1081 cgctcatgtc ctcacgatac ggcagcgggc ctggcttcgg cggagcggga gcctctggcg
     1141 ccacccgccc cacgcgaaga tgtgtctcgc tctactgctg tccgttgtcg tctttgacca
     1201 cgccgaccac gtgaccctcg gggtcggcca acagcgcgaa ggtgacaatg tccggaacct
     1261 cggtcaccgg gacgatcgtc ttgtcgccga gcgactcggc cttgtcgaga tacgcctgcg
     1321 gatcgtcgac ctgcacgtag aacgtcacgt gtgactggcc ggtctcggac gcgccgatgc
     1381 cgccgccgat cgagccctcg tggcccgtgt cgaccatgcg atagccgtcc atgccgtcga
     1441 ccgggttcac cttccagtcg aacaggttgg cgtagaagcc gccgagcgct ccgtcgtcgg
     1501 agccgatgat ctcgaagtgg actactgggt tggccattgg ggcctccttt gctcgtaaat
     1561 gggctctcca ccccggtaga cgaacgagac cgtggcaaat cgaccgtcgt tccgcatcgc
     1621 cgagcggggc ggactcaggc gacggcgccg cgcacctggc gcaccgccac ggtgatcacg
     1681 gcgaggtcgt tgatgccttc cgtgtcgagc tggtcgacga gcctgtcgag acgcccgacc
     1741 gcctccgggc gggaggcgag ataggactcg agcgcctgct ccgctgtctg ggtgtcgtcg
     1801 tcgcaggccg cgatcgcctt ctgcacggcc tcccgtcgca ccaggaggag gtcgtcgacg
     1861 acggtgttga aggcgacgcg ctcccatcgc gacgtaggct gcagtgcgac gacctgatct
     1921 tcgagccagt cgaggtgcag gctctgcccg gcctcgaaga aggcgcgcgc gacgtcggtc
     1981 acggagcggt cgctcccccg cgccacggtg acgatgtcgg ggccgtgtgt gagggcgggt
     2041 tggtactcgt tgcggcgcgc gagcgtctcg ggcgcgccgc gcgagaccag cccggcggcc
     2101 tctcgctcgc gctcgacctt cgaagcctct ggaatgagct ccgggagtac gaagtccagc
     2161 tcgttgaaac cctgtgccat ggtctccacc gtctcgccga tgtcggctcc cggtgcgttc
     2221 atcacgaacc agcgcgcggt catctcgacg agccagtcca ctcccatcat caactcgttc
     2281 tgcacgaccg ggtcgatgac gccgtcgagc gcctcgatgt cggcccagcg cgtctcggcc
     2341 gcggtcacct cgcgcgcgat gcggtacgcg cggatgacgt ccgggggctc ggcgcccgtc
     2401 tcggcgacga accgccagac gaacgtgatc ccctgtgagt cgacgacgtc gttcgagacg
     2461 atcgtcgcga tgaggtcgcg gcggagcgga tgctccttga tcaggtcgtc gaaccgctct
     2521 gcgacgcgcg gggggaagta cgcgcgtaga tcgcgttcga gatagggatc gtccgggagc
     2581 gaggacggaa gcacccatcg cttgaggctg cgctttgcgt acgccagcag cacggcgagc
     2641 tcgggtcgcg ccatcccgag cccggcccgc tcccgttcag ccatctcctc gctcgacggc
     2701 aggtactcga tcgggcggtc gagcagcccc tggtcctcga gcgaccgcat cacgtcctcg
     2761 aactcgcgga tccgcttcgg cgagatcgcg accttctgcg agaggatctg agcctgcagg
     2821 tagttgtggt acaggacgtg tcgtgtcacg tcctccgcgc tttctgcgag gagctcgttg
     2881 cgctgcttgc gggtgaggtc gcctcgctcc atcgccagac cgagcaggat cttgaggttc
     2941 acctcgtggt cggagcagtc gacgccgccg gagttgtcga tgaagtccgt gttgatcctc
     3001 ccgccggcgt ttgcatactc gatgcgtccg cgctgggtga aaccgaggtt gccgccctcg
     3061 ccgacgacga gcgcgcggac gtcccggccc gtgacgcgca tcgcatcgtt cgctcgatcg
     3121 ccgacctcgg cgtctgtctc gtcggtcgcg cggacgaagg tgccgacgcc gccgttccac
     3181 aggaggtcga cgggtgcgcg caagatcgtc gagatcaact ccgtcggggt caccgacccc
     3241 ggctccgttc ccagagcctc ggccgcctcc tccgagatgg agatgctctt ggcactccgt
     3301 ggccagatgc cgccgccccg tgagatgagg tctcggtcgt agtcgtccca ggtgctccgg
     3361 ccgagctcga acagccgctt ccgctccgcg aagctcgtga tcggatcggg cgtcgggtcg
     3421 aggaagacgt ggcggtggtc gaatgcggcc accaggcgga tctgctccgt gtagagcatg
     3481 ccgttgccga agacatcgcc cgacatgtcg ccgatgccgg cgacccggat cgggtccgtc
     3541 atgacgtcgc ggccgagctc gcggaagtgc cttttgaccg actcccacgt ccctcgcgcg
     3601 gtgatcccga gcttcttgtg gtcgtagccg gtggaaccac ccgaggcgaa cgcgtcgtcg
     3661 agccagaagc ggtattcctg cgagatcgcg ttcgcgatgt ccgagaacga cgctgtcccc
     3721 cggtcggcag cgacgacgag gtaggggtcg gcctcgtcga gaatccggac gtcgggcggg
     3781 tgcacgacct ccccgtcggc gaggctgtcg gtgaggtcga gcagacctcg gatgaaggtc
     3841 tcgtactgcc gccgcacctc gtctctcgtt tcctgtggag gcacctcgcg cttcagcacg
     3901 aacccgccct tggagccact cgggacgatc acggcgttct tcgtcgtctg cgtcttcatc
     3961 agatccagga tctccgtgcg gtagtcctcc tgccggtcgg accagcggac gccgccgcgc
     4021 gctacctgcc ggaagcggag atggatcccc tccatctcgg gcgagtagac gaagatctcg
     4081 tacaggggat acggcttcgg gacatcgggc acctgctcgg agcggatctt gaagctgagg
     4141 tagcgtcgcc cgggccggaa cgcgttcgtc cgcacaatcg cctcgatcac ctgcaggtag
     4201 ccgcgcagga tgcggtcgtg gtcgagtgac ggcaccgagt cgagcttctc cactatgcgc
     4261 tcccgcaact cggcctcggc gtcctcgtcg cgaggccgcg acggatcgaa ccggagctcg
     4321 aagaagcgaa tcaggtcggc ggcgatctcc gggttggccg cgaaggcagc gttctcgtac
     4381 tcgagcgtga aggaggcgct tacccggcgg gcgtaggtcc gcagcgcgcg caggacggcg
     4441 acctgcggcc aggtgagccc ggccagcacg atgaggcggt ccaaagagtc gctctcggcc
     4501 tcgcgtcgcc acacggcgcc cagcgcgttg gcgacgagct cgccgagcgc cgggacgtcc
     4561 acggcttctc cgtccgggcc cacgacgccg aagttgtgga tgtggatgtc cgcgtcgccc
     4621 tcgacggcga tgggaacctc ctcgacgacg accagcccca gcgcctccag cccgggcatc
     4681 agctccgaga ggttcgcgcg tccgccggtc ttgtagagat gcacccgtgt ctccggcctc
     4741 tggccccgct cgttccgaag ggcgaccacg aacggctgct gcccgccgag ctgctcgagg
     4801 tgaccgatgt cgatcacggc tcgatacacg tcggtcgcag acttgtagta gtcgggcagc
     4861 cgttccgacc acttggtcgc cagctcgccg ccgtgctcct cgccgtgcag cgcaacgaga
     4921 cgctcgagca ggcggtcgtc ccaggtccgg gtgagggcga tcacctcgtg ctcgagtgcc
     4981 gcgtagtcca cgtcggggat cgctccgtgc ccgacgtgga tgcggaagtg gatcaaggcc
     5041 tgatcggact ggccgagcga caggtggtag tcgacgctgg agccgtggaa gcgctcgcga
     5101 aacagcgcct ggaggcgctt ccgcagcggc gcgttgaagc ggtcgcgcgg cagggcgacg
     5161 agcaccgaga cactccggtc gaagaggtcg cggtgggcaa agagccgtac tttctgctgc
     5221 tcctgcaggg agatcagctc cgcgaccgtt ctctgcagag tttgttgatc gacggagagc
     5281 agctcgtcga gcgggaagtt ctcgatcagc tcgacggccg ccttgtgatc gtgcgagccg
     5341 ggaacgaagt cctcggccgc caggacccgc tgcagcttga gacgcaggag cggtatgcgc
     5401 ccggccggct ccgaataggc cttccgtgtg aacagcccga ccattcgcag ctcgcccagc
     5461 acggcgccgt cgggcgagac cgacttgacg ccgatgtagt ccatcttggc gcggcggtgg
     5521 acggtcgcgt tccggttggt cttggagacg aggagcagct
//
LOCUS       DBNGNK010000350         5616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1637396,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000350 DBNGNK010000000
VERSION     DBNGNK010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1637396"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /gene="gatA"
                     /locus_tag="AC150V_10380"
     CDS             <1..1216
                     /gene="gatA"
                     /locus_tag="AC150V_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR3763364.1"
                     /translation="STADVRTTCGSAFLENYVPPWDATVVARLRAAGSVFLGKTNTDE
                     FAMGSSTENSAYGPSRNPWDTERVPGGSGGGSASAVSAGLAPWALGSDTGGSIKQPSA
                     LCGNVGLRPTYGTVSRFGVVAFASSLDQVGPVARTVRDCALLYSIISGRDEADSTTVE
                     RPGPVRLPERDRLDGIRIGIPRELNEHPGIEPGVTAAVERALGLAQELGAELGECGLP
                     RSVEYGLPCYYLIAPAEASSNLARYDGVRYGPRADGDSYRDMVERSRNEGFGPEPKRR
                     IMLGTYALSAGYYDAYYGQAQKVRTLIVDEHRAAFEHFDVLASPTSPTVAFKIGERAA
                     DPLAMYASDLLTIPSCLAGLPGLSVPVGLSEGLPVGLQLIGPQFGENVLFDVGYALER
                     AIGFDTVPERLR"
     gene            1213..2622
                     /gene="gatB"
                     /locus_tag="AC150V_10385"
     CDS             1213..2622
                     /gene="gatB"
                     /locus_tag="AC150V_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR3763365.1"
                     /translation="MSWEPVIGLEIHVQLKTRTKMFCRCPVGFGAGENTQTCPVCLGF
                     PGALPVPNRRAIEWTLKLGLALGCEIAPHAVFARKNYFYPDLPKGYQISQYDLPSCIN
                     GKVIVPTSEGDHVIGIVRAHLEEDAAKTTHIGGRSGRIGGADYSLVDFNRGGTPLVEI
                     VTEPDLHSAEDAKRFLQLLRQTIVELGISDAEMEKGTLRVDANVSVRPVGSAELRTRA
                     ELKNMNSFNYVARGIEAEVERQIGIWESGGEVVQETLDYDAATGGLTPRRAKEEADDY
                     RYFPEPDLVPVEPSPDLVNALRAELPELPAARIRRIEEGIGLDRAVVLVTGGLDALWQ
                     ETVAAGADPGAAANLIANQLVGAGVAPASVSPAELAKLVEARDRIPRQALDEAIARAG
                     EPGFSADPYLAQEAVSDTDELGPVIEAILAANAEQVEQYRGGKEGLLGYFVGQVMKET
                     QGKANPRVVNELVREKLSG"
     gene            2657..3727
                     /locus_tag="AC150V_10390"
     CDS             2657..3727
                     /locus_tag="AC150V_10390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007456563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HIR3763366.1"
                     /translation="MALASGPAITTEELQLAARNHAMPLEALDWDLTPVGLHYLLIHF
                     DIPLVDVREWRLTVDGLVTKRLELTLDELRARPATDLVATMECAGNGRAMLEPRPISQ
                     PWLTGAVGTAHWRGVELASLLDEACPDDEAVEFVFTGLDRGVDGGIEQDYARSLTVAD
                     AMSSGAVLAYEMNGAPLQPQHGFPLRMVVPGWYGMTNVKWLTSIEAIAEPFDGYQNTS
                     AYRITRDENDPGRPVDRIQPRSLMRPPGIPHFETRSRLLRRGPVVLGGRAWSGKAPIA
                     RVDVSTDGGLTWQDADVRRDFDSPWAWCSWRATWEPQPGNHVLCCRAHDEAGNSQPLE
                     ADWNLGGYENNAVQRLPVVVAE"
     gene            3724..4140
                     /locus_tag="AC150V_10395"
     CDS             3724..4140
                     /locus_tag="AC150V_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR3763367.1"
                     /translation="MRHATDYLSFETPERRGYINITPQVEEFVSRSDISHGLCLVNPM
                     HITAAVFVNDDEQGLLADLDEWLERLAPHEPVSSYRHNVGEDNADAHLKRHLMSHQVV
                     LAITDGTLDLGPWEQVFYAEFDGRRRKRVVLKAIGD"
     gene            4175..4690
                     /locus_tag="AC150V_10400"
     CDS             4175..4690
                     /locus_tag="AC150V_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763368.1"
                     /translation="MRRGLVWAFTLPLVAGGVLGAHALAYAIVRAPAADFHGYLGHAP
                     QLIAVLATLAACTLAVQGRTRAPVPRDFVVLALAAFFAQEHLERFVHTGELPWLLTRP
                     EFLLGLILQLPAALLVWLLARFFLVFEPVASQRPPRLSTLSLGLLLRRYGFSQRPLCV
                     ALAARGPPVLP"
     gene            5033..5281
                     /locus_tag="AC150V_10405"
     CDS             5033..5281
                     /locus_tag="AC150V_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763369.1"
                     /translation="MTVRQGRVVGGIKRPKVQKGEQVLLVVKTDAGESVHLHGYDVDE
                     TVVAGKPTRITFEASIPGRFELELHNPDVLLADIQVNP"
     gene            5290..>5616
                     /locus_tag="AC150V_10410"
     CDS             5290..>5616
                     /locus_tag="AC150V_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fenitrothion hydrolase"
                     /protein_id="HIR3763370.1"
                     /translation="MEDVLAESRSMSFVAHGIGSIRDLPVPAWLFFWGAAVVLVVSFV
                     LLGVLWKQPLLARRAGRALPTGVSRLVLGPLRVALQVVSALLFLLVFVSARVGDTDPF
                     DNLAPTW"
BASE COUNT          839 a         1975 c         1900 g          902 t
ORIGIN      
        1 ctcgaccgcc gatgtccgca ccacctgcgg ttcggctttc ctggaaaact acgtcccccc
       61 ctgggacgcg acggtggtcg cgcggctccg cgcggccggc agcgtgtttc tgggcaagac
      121 caacaccgac gagttcgcga tgggctcctc caccgagaac tcggcctatg ggccgtcgcg
      181 caacccctgg gacacagagc gtgtccccgg aggatcgggt ggcggctccg cgtcggcggt
      241 gagcgccggc ctggcgccat gggcgctcgg ttcggacacc ggcggctcga tcaagcagcc
      301 gtcggcgctc tgcggcaacg tcggcctgcg gccgacgtac ggaaccgtct cgcgcttcgg
      361 cgtcgtcgcg ttcgcgtcga gtctcgacca ggtcgggccg gtcgcgcgga cggtgcgcga
      421 ctgtgcgctc ctctactcga tcatctcggg tcgcgacgag gccgactcca cgacggtcga
      481 gcggcccggg ccggtgcggc tccccgagcg tgacaggctg gacggaattc ggatcgggat
      541 cccgcgagag ctgaacgagc atcccggcat cgagcccggc gtcacagcgg cggtcgagcg
      601 ggcgctcggg ctcgcgcagg agctcggcgc agagctgggc gaatgcgggc tgccgcggtc
      661 agtcgagtac gggcttccct gctattacct gatcgcgccg gcggaggcgt cgtcgaacct
      721 ggcccgatac gacggcgtcc gctacgggcc gcgcgccgac ggcgacagct atcgggacat
      781 ggtggagcgg agccgcaacg agggcttcgg tccggagccc aagcgccgca tcatgcttgg
      841 cacctacgcg ctttctgccg gctactacga cgcgtactac ggccaggcgc agaaggtgag
      901 gacgctgatc gtcgacgagc acagggccgc cttcgagcac ttcgacgtgc ttgcttcgcc
      961 gacgtctccg accgtcgcgt tcaagatcgg tgagcgcgcc gcggatccac ttgcgatgta
     1021 tgcgtccgac ctcctgacga tcccgtcgtg cctagccggc ctgcccgggc tgagcgtccc
     1081 cgttggactc tccgagggtc tgccggtcgg cttgcagctg atcggcccgc agttcggcga
     1141 gaacgtcctc ttcgacgtcg gctacgcgct cgagcgggcg atcggcttcg acaccgttcc
     1201 ggagcgcctg cgatgagctg ggaaccggtc atcgggctgg agatccacgt ccagctgaag
     1261 acgcggacga agatgttctg tcgctgcccg gtcggcttcg gggcggggga gaacacgcag
     1321 acctgccccg tttgcctggg ctttcccgga gccctgccag tgccgaacag gcgggcgatc
     1381 gagtggacgc tcaagctcgg gctcgcgctc ggctgcgaga tcgcgccgca tgcagtcttc
     1441 gcgcgcaaga actacttcta tcccgatctc cccaagggct atcagatctc ccagtacgat
     1501 ctaccttctt gcataaacgg caaggtgata gtaccgacaa gcgaaggtga ccacgtgata
     1561 gggattgtga gggcgcacct cgaggaggac gccgccaaga cgactcacat cggcggtcgc
     1621 agcggacgca tcggcggcgc tgactactcg ctcgtggact tcaaccgcgg cggcacgccg
     1681 ctcgtcgaga tcgtgacgga gcccgacctg cactctgcgg aagacgcgaa acggttcctg
     1741 cagctgctcc gccagacgat cgtcgagctc ggcatctcgg atgccgagat ggagaaaggg
     1801 acactgcgtg tcgacgccaa cgtctctgtg cgcccggtcg gatcggccga gctccggacg
     1861 cgggccgagc tgaagaacat gaactccttc aactacgtgg cccgcggcat cgaagccgag
     1921 gtcgagcggc agatcggcat ctgggagtcc ggcggagaag tcgtccagga gacgctcgac
     1981 tacgacgcgg cgaccggcgg tctcaccccg cgccgcgcga aagaggaggc cgacgactac
     2041 cgctatttcc cggagcccga cctcgtcccc gtcgagccat ctcccgacct cgtgaatgcg
     2101 ctccgcgccg agctgccgga gctgccggcc gcgcgcatcc gccggatcga ggagggcatc
     2161 ggcctcgacc gtgccgtcgt gctcgtcacc ggcggcctgg acgctctctg gcaggagacc
     2221 gtggcggccg gtgccgatcc gggcgccgcc gcgaacctca tcgccaacca gctcgtcggc
     2281 gcgggcgtcg cgccggcatc ggtgtcgccc gccgagctgg cgaagctcgt cgaggcccgc
     2341 gaccgcatcc cgcgccaggc gctcgacgag gcgatcgccc gggcgggtga accggggttc
     2401 tcggccgacc cctatcttgc acaggaagca gtttccgaca ccgacgagct ggggccggtg
     2461 atcgaagcga tcctggccgc caacgccgag caggtcgagc agtaccgagg cggcaaggag
     2521 ggtttgctcg gctacttcgt cggccaggtg atgaaggaga cgcaggggaa ggccaacccg
     2581 cgcgtcgtca acgagctcgt gcgagagaag ctgtccggct agacgaacgg cgggggcctg
     2641 cgctacgttc ccggccgtgg cgctcgcctc cggtccggcc ataaccaccg aggagctcca
     2701 gctcgcggcg cgcaaccacg cgatgccgct cgaggcgctc gactgggacc tcaccccggt
     2761 gggtctccac tacctgctga tccacttcga catcccgctc gtcgacgtcc gcgagtggcg
     2821 actgaccgtc gacggactgg tgacgaagag gctggagctc acgctcgacg agctccgcgc
     2881 ccgcccggca accgatctgg tcgcgactat ggagtgcgcg ggaaacggta gagccatgct
     2941 cgaaccgcgc ccgatcagcc agccctggct cacgggcgcc gtcgggacgg cgcactggcg
     3001 tggcgtcgag ctcgcctcgc tcctcgacga ggcgtgtccc gacgacgagg cggtcgagtt
     3061 cgtcttcacg ggcctcgatc gtggcgtcga cgggggcatc gagcaggact acgcacggag
     3121 cctcaccgtc gcggacgcga tgagctccgg tgccgtgctc gcctacgaga tgaacggcgc
     3181 gccgctccag ccgcagcacg gcttcccgtt gcggatggtc gtccccgggt ggtacgggat
     3241 gaccaatgtc aagtggctga cgtcgatcga ggccatcgcc gagccgttcg acggctacca
     3301 gaacacttcc gcctaccgca tcacgcgcga cgagaacgac ccggggcgcc ccgtcgacag
     3361 gatccagcca cgctccctga tgcggccgcc cgggatccct catttcgaga ctcgctcacg
     3421 gctccttcgc cgaggaccgg ttgtgctcgg agggcgggcg tggtcgggga aggcgccgat
     3481 cgcccgcgtc gacgtgagca ccgacggcgg cctgacgtgg caggacgcag acgtgaggcg
     3541 ggacttcgac tcgccctggg cctggtgctc gtggagagcg acgtgggagc cgcagccggg
     3601 caaccacgtc ctctgctgcc gcgcccacga cgaggccggt aactctcagc cgctcgaagc
     3661 ggactggaat ctcggcggct acgagaacaa cgccgtgcag aggctgccgg tcgtcgtcgc
     3721 cgagtgaggc acgccaccga ttacctctcg ttcgagacgc cggagcgccg cggctacatc
     3781 aacatcaccc cgcaggtaga ggagttcgtc tcgcgttcgg acatctcgca cggcctctgt
     3841 ctcgtcaacc ccatgcacat caccgcggcc gtcttcgtga acgacgacga gcagggcctg
     3901 ctcgcggatc tggacgagtg gctcgagcga cttgctccgc atgagcctgt gtcgtcgtac
     3961 cgccacaacg tcggcgagga caatgcagac gcgcacctga agcggcatct catgtcgcat
     4021 caggtcgtcc tggcgatcac cgacgggacg ctcgacctgg gtccctggga gcaggtcttc
     4081 tacgcggagt tcgacggacg ccgacgcaag cgggtcgtgc tcaaggcgat cggcgactag
     4141 ccctaccgcg ccgcgcggat ggcattatcg gcagatgcgc cgggggctcg tctgggcgtt
     4201 caccctgccg ctcgtcgccg gcggggtgct cggcgctcac gcgctcgctt atgcgatcgt
     4261 gcgcgcgccg gctgcggact tccacggcta cctcgggcat gcgccgcagc tgatcgcggt
     4321 gcttgccacg cttgccgctt gcacgctcgc ggtgcaggga cgcacgcggg ctcccgtgcc
     4381 gcgggacttc gtcgtgctcg cgctcgccgc cttctttgcg caggaacacc tggaacggtt
     4441 cgtacacacg ggcgagctgc cctggttgct gactcggccg gagttcctgc tcggtctgat
     4501 cctgcagctc ccggcggcgc tcctggtgtg gctgctcgcg cgcttcttcc tcgtattcga
     4561 gccggttgca tcgcagcggc cgccccgtct ctcgacgctt tccctggggc ttctcttgcg
     4621 ccggtacggg ttctcccagc gtccgctgtg cgtcgccctt gcggcccgtg ggccacccgt
     4681 cctcccgtga gccgactaaa tcggctgccc gctgcagccc gactgaaccg gaggacgaca
     4741 gacgatgcga tcccgtaccg cgaaagcgct acttctctcc attctcggcg gcgtgctggt
     4801 cggcgcagcc gccgcgtgtg gaggtagcga cagctcagca ccgaccaccg cctcgcagac
     4861 gccgccgccg ccaccgtcaa cggcaccgac gccaccgccg ccgaccccgc cgccacctcc
     4921 tgcgccgccg ccgccgccga tcgagccgcc tcctccgcct ccgccccctc ccacgcgccc
     4981 gccaccgccg cccgctccgt caccggcgaa gccgaagccg cagacggtca ccgtgacggt
     5041 gcgccaggga cgggttgtcg gcggcatcaa gcggccgaag gtgcagaaag gcgagcaagt
     5101 gcttctcgtc gtgaagaccg acgctggcga gagcgtgcat ctccacggct acgacgtcga
     5161 cgagaccgtc gtcgcgggga agccgacgcg gatcacgttc gaggccagca ttcccggtcg
     5221 cttcgaactc gagctccaca accctgacgt gttgctcgcc gatatccaag tgaacccgta
     5281 gcagttccgg tggaggacgt tctggcagag agccggtcga tgagcttcgt cgcccacggc
     5341 atcgggagca tccgcgacct ccctgtgccg gcgtggctct tcttctgggg ggcggcggtc
     5401 gtgctcgtcg tgtctttcgt cctgctcggg gtgctctgga agcagccgct gctggcccga
     5461 cgcgcgggcc gggcgcttcc gacgggtgtc tcacgcctcg tcctcgggcc gcttcgggtt
     5521 gccttgcagg tggtctccgc gctgcttttc ctgctcgtct tcgtgtcggc gcgggtcggt
     5581 gacaccgatc cgttcgacaa ccttgcgcct acctgg
//
LOCUS       DBNGNK010000351         8420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_632331,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000351 DBNGNK010000000
VERSION     DBNGNK010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_632331"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1222)
                     /gene="cysS"
                     /locus_tag="AC150V_10415"
     CDS             complement(<1..1222)
                     /gene="cysS"
                     /locus_tag="AC150V_10415"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR3763371.1"
                     /translation="MRLTDTLTGELLELPPQPGPIGMYACGPTVYQRAHIGNARPFVV
                     FSWLRNWLRDRDYDVTFVHNITDVNDRIYEAAPGRSAELAVEATEWYLEDIAGLGLGM
                     PDELPLATETMPEIISLIAELVEGGHAYAVDGDVYFRVAGFPEYGRLSGQRPDQAEEQ
                     EPNPLKEDPRDFALWKANKPDEDTWWESPWGRGRPGWHIECSAMAEKFLGPAFEIHGG
                     GLDLVFPHHENEVAQSRAAGRPFARIWMHNGMLRFTGEKMSKSAGNVATIRDVLDEWG
                     AETALVFFMSAHWRKPIDFSETTMAAAAAQVETLRNALRGEVRAEGDWSTLVAALDDD
                     FNTAAALAIAHDWARAGAQVELRRLLALFGLASLAERAVAPPEIVDLADQRHLAREGG
                     DFAEADRLRDVIAAK"
     gene            1272..1574
                     /locus_tag="AC150V_10420"
     CDS             1272..1574
                     /locus_tag="AC150V_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA/TatB"
                     /protein_id="HIR3763372.1"
                     /translation="MPLGIGQWEILILAAVLVLVFGTSKVPRIARELGLGLREMKKTI
                     DDVDPRKPIKELQEPLDELDPRKPIRELDEPADREATTAKRPQSEAKSRPSRSSEN"
     gene            complement(1571..2623)
                     /locus_tag="AC150V_10425"
     CDS             complement(1571..2623)
                     /locus_tag="AC150V_10425"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF012951.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR3763373.1"
                     /translation="MSVRVRFAPSPTGSLHLGNALTAVANRRFADRRKGRMVLRIDDT
                     DPARSAAGGEQAILDDLAWLGVQYEEGPVRQSDRGELYAEAATRALAAGAATDDDGSV
                     RLEGTTLLRADGTATYHLASVADDLDLGITHIIRGSDHRPNEALQQRVAAALGGRLPA
                     VIHHGLLLGADGRKLSKRHGHVSLDDVREDGIPAQAVLSYLEELDLPRHDVMLDLARL
                     KRLSTDVIGAMADEQLADAAGAPVSVVPALRGARTLVEAREYARLVVSPEAVDLPADA
                     GATLERFAELRAGAGKDLDAAGAREIVRELKAVGGDLRTLRLALTGAPRGPELWAVVL
                     ALSNEEALSRARSQSG"
     gene            2679..3704
                     /locus_tag="AC150V_10430"
     CDS             2679..3704
                     /locus_tag="AC150V_10430"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR3763374.1"
                     /translation="MGVEVPSLDIERPGAALEYLRAHDRLDDDEHPEVTVLEGGVSSR
                     AVLVRSDRRAWVLKQALAKLRVPVDWFSDPLRVHREASALRWWHVLVPGAAPELVFED
                     AREHLVCMTAVAFPHDNWKTMLLAGRVEASHIDQFAQILGTVHARSAERLAELEDEFG
                     DRTYFETLRLEPYYGYAAEQVEPAAPFLHALQDDTRAVRDSLVHGDYSPKNVLIHGDR
                     LVLVDHEVSHLGDPAFDLGFSSAHLLSKAHHLPALRGAFLGAARRYWAGYAATTEDAP
                     WRAGLEERAVRHTIGCTLARVAGRSQLEYLRPEERERQRALVLPLLEAPPTTYGGLVD
                     AWEAALA"
     gene            3701..4945
                     /gene="eno"
                     /locus_tag="AC150V_10435"
     CDS             3701..4945
                     /gene="eno"
                     /locus_tag="AC150V_10435"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3763375.1"
                     /translation="MTATIESVSALEVLDSRGRPTVWASCTLAGGATGTASVPSGAST
                     GAAEAHELRDHDPRRYGGFGCRRAVAGVRGEIADALRGVRFDDQRELDEALCSLDGTS
                     TKSRLGANALLAVSVSFARAASAAEGVPLYAHAAALAGISTPALPRPLVNLFSGGMHA
                     GGQVSIQDVQLLVESASTVDEALAAVHSVYAEATAISAERFGMRLLRADEGGLAPPFR
                     SSDEMLECAIESVLRAGLTPGEDVALALDVAAAQLYADGRYVLDGRDLDSNGMVEQAA
                     TWLRDFPVVSLEDPLDQEDWAAWAELMGRAGDALLLGDDLLCTQTSRIRAAQERHAAN
                     ALLLKPNQVGTLTEAADALRAAREGDWAVVVSARSGDTEDDWLADLAVGWAADHIKIG
                     SITQSERLAKYNRLLAIEHELA"
     gene            4942..5688
                     /locus_tag="AC150V_10440"
     CDS             4942..5688
                     /locus_tag="AC150V_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009147228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HIR3763376.1"
                     /translation="MTGRLRPVARAAGGVVFESARDLAPIVVVVAFFQLVVLRQPFPN
                     LVGVVLGLAAVLAGLTLFLRGREMGLFPLGEAMAGAFARKGSLFWLLAFAFALGLATT
                     VAEPALIAVADEAARVRADELGVERSDPSVDQYALGLRATVAVSVGVALLVGVIRIVR
                     GWPIQWFIIVGYLVVVGLTQLAPEETVGIAYDSGAVTTSTITVPLVAALGVGLARSIR
                     GRNPLIDGFGLIAFASLFPIAFVLLYGIIV"
     gene            5685..6476
                     /locus_tag="AC150V_10445"
     CDS             5685..6476
                     /locus_tag="AC150V_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008045580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIR3763377.1"
                     /translation="MSGLVDILERVGTTLRDVLPIVFTILVFQLFVLRRRLPNPRRLA
                     IGFAYVLAGLTLFIVGLEEALFPIGRTMAEQLTDPEFLIGEAAAAAGDAPKWWDYYWV
                     YLFAMAIGFSTTVAEPSLIAVSLKAEEVSGGTVGSWPLRIAVAVGVSIAVALGALRIV
                     TGTPLYVYMIVGYVVVVLQTLVAPRAIVPLAYDSGGVTTSTVTVPLVAALGLGLATTV
                     PGRSPLIDGFGMIALAALFPMIAVMGYAQLTAIRQRMGTRREEAR"
     gene            6473..6823
                     /locus_tag="AC150V_10450"
     CDS             6473..6823
                     /locus_tag="AC150V_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017008042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR3763378.1"
                     /translation="MKFKGIIALVDDGVTDAVVDAARRSGATGCTIISSARGEGLAPP
                     KTFFGLDLEGHRDVVLFLVEEHLSRDILETVGTVGHFDDQVGTGIAFQFDIEDAVGLS
                     SQIETIQHEIEDQI"
     gene            6820..7233
                     /locus_tag="AC150V_10455"
     CDS             6820..7233
                     /locus_tag="AC150V_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004748984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR3763379.1"
                     /translation="MSVRDYVTVREVMTDDPHVIDGLATVREAMNRMRNESVNSLVID
                     RRHEGDEYGIVVVGDIAEHIIGHNRSPDRTNVYEIMSKPVVSLEADMNIKYAVRLLAR
                     FGLSRGIVLEKGRLIGIVTLRDLVFRYIPQVDDDS"
     gene            7336..7611
                     /locus_tag="AC150V_10460"
     CDS             7336..7611
                     /locus_tag="AC150V_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020628947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase"
                     /protein_id="HIR3763380.1"
                     /translation="MTVVRLFVRHSVNDFTTWRKAYDNFDDERAGMGVTGHAVYQAVD
                     NPNEVTVTHDFASLDDGRSFMSSPRLKEVMDAAGVVGEPQAWFANEA"
     gene            7736..8356
                     /locus_tag="AC150V_10465"
     CDS             7736..8356
                     /locus_tag="AC150V_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017883556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD family protein"
                     /protein_id="HIR3763381.1"
                     /translation="MLQQTQVERVIPRYRRWLDRWPHVSTLAAASPADVIREWQGLGY
                     NRRALNLHRAARTIDAGGWPDDLTELPGVGPYTAAAVRNFALGEAVLPIDVNVERVMR
                     RTQHRFGPAAAQALMDLGATVCLARVPKCDACPLAPSCPSRGVREEPRRRQGAFEGSF
                     RQRRARTLRLIAESPREIDGLDGAAVQALERDGLVVETDGVVSLPR"
BASE COUNT         1270 a         2944 c         2845 g         1361 t
ORIGIN      
        1 ccttcgcggc gatcacgtcc cgcagccgat cggcctcggc gaagtcgcct ccttcacgcg
       61 ccaggtggcg ctgatcggcg aggtcgacga tctcgggcgg agccactgcc cgctcggcga
      121 gcgaagcgag cccgaagagc gcgaggagac gccgcagctc gacctgagcg ccggcgcgcg
      181 cccagtcgtg tgcgatcgcg agcgccgccg cggtgttgaa gtcgtcgtcg agcgccgcga
      241 cgagagtcga ccagtctccc tcggctcgca cctcgccgcg gagcgcattc cgcaacgtct
      301 ccacctgcgc ggccgcggca gccatggtcg tttcggagaa gtcgatcggc ttgcgccagt
      361 gcgcgctcat gaagaagacg agggccgtct ctgcccccca ctcgtcgagc acgtcgcgaa
      421 tcgtggccac gttgcccgcc gacttcgaca tcttctcgcc agtgaagcga agcatcccgt
      481 tgtgcatcca gatgcgcgcg aagggacggc ccgccgcccg cgactgcgcc acctcgttct
      541 cgtggtgtgg gaagacgagg tcgaggccgc cgccgtggat ctcgaacgca gggccgagga
      601 acttctcggc catcgccgag cactcgatgt gccagcccgg acgcccgcgc ccccagggcg
      661 actcccacca cgtgtcctcg tcgggcttgt tcgccttcca gagcgcgaaa tcgcgagggt
      721 cttccttcag cgggttcggc tcctgctctt cggcctggtc gggacgctga ccggagagcc
      781 gaccgtactc cgggaagccg gcgacccgga agtagacgtc tccgtccacc gcgtaggcgt
      841 ggccgccctc gaccagctcc gcgatcagcg agatgatctc cggcatcgtc tccgtggcga
      901 gggggagctc gtcaggcatg ccgaggccaa ggcccgcgat gtcctccaga taccactctg
      961 tcgcctcgac cgccagctcg gcgctccgtc cgggcgcagc ctcgtagatc cggtcgttca
     1021 cgtcggtgat gttgtgcacg aacgtcacgt cgtagtcgcg atcgcgtagc caattgcgga
     1081 gccacgaaaa gacgacgaaa ggccgcgcgt tgccgatatg agcccgctgg tagacagtcg
     1141 gaccgcaggc atacatgccg atcgggcccg gctgcggcgg aagctcgagc agctcgccgg
     1201 tcagcgtgtc cgtgaggcgc atcgcgcgat cgtaccgccg cacttgcagg ctcctgcaac
     1261 aatgcagagc aatgccattg ggaatcggac agtgggagat cctcatcctc gcggcagttc
     1321 tcgtgctcgt gttcggcacg tcgaaagtgc cgcgtatcgc ccgagagctg gggctcggcc
     1381 tgcgagagat gaagaagacg attgacgacg tcgacccgcg taagcccatc aaggagctgc
     1441 aagagcccct cgacgagctc gatccgcgga agccgatcag agagctcgac gagccggccg
     1501 accgggaggc gaccaccgcc aagcgccctc agagcgaggc caagtctcgc cctagccgct
     1561 cgtccgagaa ctagccggac tgcgatcgcg ctcgggagag cgcttcctcg ttcgacagcg
     1621 cgagcacgac cgcccagagc tcgggccccc gcggagcccc ggtcaacgcg agtcgaagcg
     1681 tcctgagatc tccgcccact gccttgagct cacgcacgat ctcgcgagcg ccggcggcgt
     1741 cgagatcctt gccggcaccg gcccgcagct ccgcgaatcg ctcgagcgtc gcacccgcgt
     1801 cagcgggaag gtcgacggct tccggagaga cgaccagccg ggcgtactcc cgagcctcca
     1861 ccagtgttcg cgcgcctcgt aaggccggaa cgactgacac gggagcaccc gccgcgtcgg
     1921 cgagctgctc gtctgccatc gcaccgatca cgtccgtgga gagacgcttc agacgggcga
     1981 gatcgagcat gacgtcgtgg cgcgggagat cgagctcctc gaggtacgag agcacggcct
     2041 gcgccggaat gccgtcctcg cgaacatcgt cgagcgacac gtggccgtga cgtttggaga
     2101 gcttgcgacc gtcagcgccg agtaggagtc cgtgatggat caccgccggg agccgcccgc
     2161 cgagcgcggc ggcgacccgc tgttggagcg cttcgttcgg gcgatggtcc gagccgcgga
     2221 tgatgtgcgt gatgccgaga tcgagatcgt ccgcgaccga ggcaaggtgg tacgtcgcgg
     2281 tgccgtcggc tcggagcagg gtggtgccct cgagacgaac ggagccgtcg tcgtcggtgg
     2341 cggctccggc cgcgagggcc cgggtcgctg cttcggcgta gagctcgccg cgatcgctct
     2401 gccgcacggg cccctcctcg tactggacgc cgagccacgc aaggtcgtcg aggatcgcct
     2461 gctcaccccc cgccgcgctc cgggccgggt cggtgtcgtc gattcgcagc accatcctcc
     2521 ctttgcgtcg atcggcgaac cgccggttgg cgaccgcggt cagcgcgttg ccgagatgga
     2581 gtgatcccgt cgggctcggc gcgaagcgaa cgcggacgct cacccgtcca atctaccccg
     2641 ggcgcctgtc ttctcccgac cctcgtagac tcgctccggt gggagtagag gtgcccagcc
     2701 tcgacatcga gcgccctgga gcggccctcg aatacctccg cgcccacgac cgcctcgacg
     2761 acgacgagca tccggaggtg accgtcctcg agggtggagt ctcgagccgc gccgtgctcg
     2821 tccgttccga ccggcgcgcc tgggttctga aacaggcgct cgcgaagctt cgcgtgcccg
     2881 tcgactggtt cagcgatccg ctgcgggtcc accgcgaggc gagcgccctg cgctggtggc
     2941 acgtcctcgt acccggagcc gcaccggagc tcgtcttcga ggacgcccgc gagcatctcg
     3001 tctgcatgac ggctgtcgcc tttccgcacg acaactggaa gaccatgctc ctcgccgggc
     3061 gagtcgaggc gagccacatc gaccagttcg cgcagatact cggcaccgtg catgcacgaa
     3121 gcgccgagcg gctcgccgag ctcgaggacg agttcggcga ccgaacctac ttcgagacgt
     3181 tgcgactcga gccgtactac ggctacgcag ccgagcaggt cgagcccgcc gccccgttcc
     3241 tgcacgcgct gcaggacgat acgcgagcag tgcgggactc gctcgttcac ggcgactaca
     3301 gcccgaagaa cgtgttgatt cacggcgacc gcctcgttct cgtggaccat gaggtgtccc
     3361 atctcggcga tcccgccttc gacctcggct tctcgagcgc ccacctgctc tccaaggccc
     3421 atcacctacc ggctctgcga ggcgccttcc tcggcgccgc acggcgctac tgggccgggt
     3481 acgcagccac gaccgaggat gcgccctggc gggcgggact cgaggaacgg gcggtgcgcc
     3541 acacgatcgg ttgcacgctc gcccgcgtcg cgggccgctc gcagctggag tacctgcggc
     3601 cggaggaacg cgagcgccag cgcgcgctgg tactaccgct gctcgaggcg ccgccgacca
     3661 cctacggagg gctcgtcgac gcgtgggagg cggctctcgc gtgacagcca cgatcgagag
     3721 cgtgtcggcg cttgaggtcc tcgacagccg tggccgccca accgtctggg cttcctgcac
     3781 gctcgcgggc ggcgccaccg gcaccgcctc ggtgccctcc ggcgcctcga cgggcgccgc
     3841 cgaggcccat gagttgcggg atcacgaccc gcgccgctac ggcggcttcg ggtgccgtcg
     3901 cgcagtcgcc ggcgtgcggg gtgagatcgc cgacgccctg cgaggcgtgc gcttcgacga
     3961 ccagcgcgag ctcgacgaag cgctctgctc gctcgacgga acgtcgacga agagccgtct
     4021 gggggcaaac gcgcttctcg ccgtctccgt gtccttcgca cgcgcggcgt ctgcagcgga
     4081 aggagtcccg ctgtatgcgc acgcggcggc gctggccggg atctcgacgc ccgcgctgcc
     4141 gcgaccgctc gtcaacctct tctcaggcgg gatgcacgct ggcggccagg tctcgatcca
     4201 ggacgtccaa ctgctcgtgg agtcagcctc gaccgtggac gaggcgctcg cggccgtcca
     4261 ttccgtctac gcggaggcga cggcaatctc tgccgagcgc ttcggcatgc gactgcttcg
     4321 ggcggacgag ggcggcctgg ctccaccgtt tcgctcctcg gacgagatgc tcgagtgtgc
     4381 gatcgagtcc gtcctccggg ccggactgac acccggggag gatgtcgccc tcgcgctcga
     4441 cgtcgccgcc gctcagctct atgccgatgg gcgctacgtg ctcgacgggc gcgacctcga
     4501 ctcgaacggc atggtcgagc aggctgcgac ctggctgcgc gacttccccg tggtgagcct
     4561 cgaagatccg ctcgaccaag aagattgggc ggcctgggcg gagctcatgg gccgagccgg
     4621 tgacgcgctg cttctcggcg acgacctgct ctgcacacag acatcgcgga tcagagcggc
     4681 ccaggagcgg catgcggcga atgcgctcct gctgaagcca aaccaggtgg gcacgttgac
     4741 cgaggccgcc gacgccctgc gtgcggcacg tgaaggcgat tgggcagtcg tggtcagcgc
     4801 acgcagcggc gacaccgagg acgactggct tgcagacctg gctgtcggct gggccgccga
     4861 ccacatcaag atcggctcca tcacgcagtc ggagcgcctc gcgaagtaca accgcctgct
     4921 cgcgatcgag cacgagctcg catgaccggc cgcctgcgcc ccgtggcgcg agcggcaggc
     4981 ggcgtggtct tcgagagcgc ccgagatctc gccccgatcg tcgtcgtcgt cgccttcttc
     5041 cagctcgtcg tgctgcgaca gcccttcccg aacctcgtcg gcgtcgtgct cgggctcgcc
     5101 gccgtcctgg ccgggctgac cctgttcttg cgggggcgcg agatgggcct gttcccgctc
     5161 ggtgaggcca tggccggcgc cttcgcgcgc aagggaagcc tcttctggct gctcgccttc
     5221 gccttcgccc tcggcctcgc gacgacggtc gccgagcctg cgctgatcgc cgtcgcggac
     5281 gaggccgccc gcgtgcgggc ggacgagctc ggcgtcgagc gttccgaccc cagcgtggat
     5341 caatacgccc tcggcctgag ggcgaccgtt gccgtctcgg ttggagtggc gctcctcgtg
     5401 ggcgtgatcc ggatcgttcg cggctggccc atccagtggt tcatcatcgt cggttacctc
     5461 gtcgtcgtcg ggctgacaca gctggcccct gaggagaccg tcggcatcgc gtacgactcg
     5521 ggcgcggtca cgacatcgac gatcaccgtg ccgctcgtcg ccgcgctcgg tgtcggcctc
     5581 gctcgttcga tccgcggccg caatccgctg atcgacggct tcgggctgat tgccttcgcc
     5641 agccttttcc cgatcgcctt cgtccttctc tacggaatca tcgtgtgagc ggcctcgtcg
     5701 acatcctcga gcgggtcggg acgacgctgc gcgacgtcct tccgatcgtc ttcacgatct
     5761 tggtcttcca actcttcgtg ctgcggcgac gactgccaaa cccgcgccgg ctggcgatcg
     5821 gcttcgccta cgtcctggcc gggctcacgc tcttcatcgt cggcctggag gaagcgctct
     5881 tcccgatcgg gcggacgatg gccgagcagc tcacagatcc cgagttcctg atcggcgagg
     5941 cggccgccgc ggccggcgac gcgccgaaat ggtgggacta ctactgggtg tacctgttcg
     6001 cgatggcgat cggcttctcg accacagtcg ccgagccgtc gctgatcgcc gtctcgctca
     6061 aggcggagga ggtctccggc ggcaccgtcg gctcgtggcc gctgcggatc gccgttgccg
     6121 tcggagtcag catcgccgtt gccctcggcg cgctccgcat cgtcacgggc accccgctgt
     6181 acgtctatat gatcgtcggc tacgtcgtgg tcgtcctgca gacccttgtc gcccccaggg
     6241 ccatcgtccc gctggcatac gactcgggtg gcgtcaccac gtcgaccgtc accgtcccgc
     6301 tcgtggcggc tctcggcctc gggctcgcca ccaccgtgcc cgggcggagc cccctcatcg
     6361 acggcttcgg gatgattgct ctcgcggccc tgttcccgat gatcgcggtc atgggctacg
     6421 cacagttgac ggccatccgc caacggatgg gcacgcgccg cgaggaggca aggtgaaatt
     6481 caaggggatc atcgcgctgg tcgacgacgg cgtgaccgac gcggtcgtcg acgcggctcg
     6541 taggagcggt gcgaccggct gcaccatcat ctcgagcgcg cggggagaag gactggcacc
     6601 gccgaagacg ttcttcggtc tcgacctgga gggccaccgg gatgtcgtgc tgttcctcgt
     6661 cgaggagcat ctgagcaggg acattctcga gaccgtcgga acggtcggcc acttcgacga
     6721 ccaggtcgga accgggatcg cctttcagtt cgacatcgag gacgcagttg gtctctcctc
     6781 gcagatcgag accatccagc acgagatcga ggatcagata tgagtgtgcg cgattacgtc
     6841 accgtgcggg aggtcatgac ggacgacccg cacgtgatcg acggcctcgc caccgtgcgc
     6901 gaggcgatga acaggatgcg gaacgagagc gtgaattcgc tcgtgatcga ccggcgccac
     6961 gagggcgacg agtacggcat cgttgtcgtc ggagacatcg ccgagcacat catcggccac
     7021 aaccggtcgc ccgaccgcac gaacgtctac gagatcatgt ccaagcccgt tgtgagcctc
     7081 gaggccgaca tgaacatcaa gtacgcggtg cggctgctcg cccgcttcgg cctgtcacgg
     7141 ggcatcgtgc tcgagaaggg ccggctgatc gggatcgtca cgctccgcga cctcgtcttc
     7201 cgctacatcc cccaggtgga cgacgactcg tgaacgacaa ttcgggggcg tcatcgcagt
     7261 cctcgagtgg cggcgtcgcg ccgcgcgctg aggcgcgggt gtagggtcag cgaatcgacc
     7321 tcgacggagg aggaaatgac cgtggttcga ctcttcgtac gtcattcggt gaacgacttc
     7381 acaacctggc gaaaggccta cgacaacttc gacgacgagc gcgcgggtat gggcgtcacc
     7441 ggtcacgccg tctatcaggc ggtggacaac ccgaacgagg tcaccgtcac gcacgacttc
     7501 gcgagtctcg acgacggccg ctcgttcatg tcgtcgccgc gcctgaagga agtgatggac
     7561 gccgcggggg ttgtcggcga gccgcaggcc tggttcgcga acgaggcctg aggtcaccgc
     7621 ggcgcgaacg gccgccccgc tcgaccagct tctgctcgac tggttcggtc gcgcggggcg
     7681 agatctcccc tggcgtcgaa cgcacgaccc gtacgcgatt ctcgtcagcg aggtgatgct
     7741 gcagcagacg caggtcgagc gtgtgatccc ccgctatcga cgctggctcg accgctggcc
     7801 gcacgtgagc acgcttgcgg cggcctcgcc agccgacgtg attcgcgaat ggcaggggct
     7861 cgggtacaac cgtcgggcgc tcaacctcca ccgggccgcc cggacgatcg acgccggcgg
     7921 ctggcccgat gacctgaccg agcttcccgg agtgggcccg tatacggcgg cggccgtgcg
     7981 taacttcgct ctgggcgagg ctgtcctccc gatagacgtg aacgtcgagc gggtcatgcg
     8041 caggacgcag caccgtttcg gcccggccgc ggcgcaggcc ctgatggatc tcggggcgac
     8101 cgtctgcctg gcccgcgtcc cgaagtgcga cgcctgtccg ctcgctccct catgtccttc
     8161 gcgcggcgtc cgcgaggagc cgcgccgccg ccaaggtgcg ttcgaaggct cgttccggca
     8221 gcgacgcgcc cgcaccttgc gactgatcgc cgagtcgcct cgcgagatcg acgggctcga
     8281 cggggcggcg gttcaggcac tcgaacgcga cggcctggtc gtcgagacgg acggcgtcgt
     8341 ctcgcttccg cgttaacccc tccgtaaggc atcgtcgcgc acaatgcggg ggtggcaacc
     8401 gttctcgtcg tagacgacga
//
LOCUS       DBNGNK010000352         5947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_61549,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000352 DBNGNK010000000
VERSION     DBNGNK010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_61549"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1856)
                     /locus_tag="AC150V_10470"
     CDS             complement(9..1856)
                     /locus_tag="AC150V_10470"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF044997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR3763382.1"
                     /translation="MRRRGLVVLAVAALAIAGGCSGGESDSYLIEGCTRKADARSVAR
                     VWDEALLDAIRRDFPAPTVHARNLFHTSAAMWDAWAAYDDIADGYFVHEKHKAEDVRA
                     AREAALSYAAYRVLLHRYSLATGLEETFSQLVTTMESLCYRIDYTSREGDSPAALGNR
                     IAAAVIEQGRDDGSNEAQRYADPAYRPVNSPLVVAKAGAALRDPNRWQPLALAQQITQ
                     NGLVVPAQVQQFVGSQWGHVTGFALPRSPEGLPIDPGPPPALGEAATDAEYKAAAVEV
                     LRFSSMLDPDDGDVIDIGPGSRGNNRLGTNDGTGHPKNVRTGEPYEANPTLRADFGRV
                     IAEFWADGPDSETPPGHWNTLANAVSDSPEISARIGGEGERVDRLEWDVKLYFALNGA
                     LHDAAVSAWGAKAFYDYVRPISMIRYMGEKGQSSDPSAASYHARGLPLVPGLIEVVSE
                     KSRARGGRHQGLRVGEIAVRGWRGNPEDPTSERAGVGWIRAVDWVPYQRPTFVTPAFA
                     GYVSGHSTFSRAGAEVLSAFTGDPAFPGGLLEWTVPAGKLLHEQGPAEDVTLQWATYF
                     DAADEAGISRLHGGIHVRADDLEGRIMGATCGKDAWALARRYFDGSARA"
     gene            complement(1853..3571)
                     /locus_tag="AC150V_10475"
     CDS             complement(1853..3571)
                     /locus_tag="AC150V_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR3763383.1"
                     /translation="MRAAVLIRFVLALAAAALAVSACTSESTDESVDRAASRPPSDGF
                     ELRDVAVQAGLDFRHGAFRWETTPDPAAMMGGGVCWLDYDRDGWLDLFVVNSYAEQEL
                     GKWKENGGPPTTALFRNSEGTFSSVGDDAGASYPVRGNGCVAADLDLDGNTDLFVTTA
                     RENRLLWNEGDGTVREGARDAGVEAYGWHSGASVGDVNRDGWPDLFVAGYADPNNRKP
                     DANGGFPNTVLGVRDLLYLSDGGRDGGRVSFVEVGVEAGLEATGFEYGLGSLFSDFDL
                     DGDLDLYVANDTNPNRLYTNVPWPGGVEADPAGLGFRFEERAARAGVADPNAGMGVAA
                     GDYDGDGGIDLFVTNLRNQTHAVFRSAEPRGTGPTFTDEREVFGPPLGDSTGWGVSWA
                     DLDLDTDLDVVLFNGHVPLTDLLADSQSPLAFENLTAQGRVGRFADWGTETGLDALGL
                     LHGRGSAAADYDNDGDLDIAVNSVASPLVLLENTGPAGHWLEVGLAGFYPGARVTAVL
                     PNGRKLVRELQAGSSYLSSEDPRLNFGLGEAREVSRLTVRWPDGTETSLDMVPSDQLL
                     VVEKPE"
     gene            complement(3597..4892)
                     /locus_tag="AC150V_10480"
     CDS             complement(3597..4892)
                     /locus_tag="AC150V_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR3763384.1"
                     /translation="MTQENGEPTAPESPDTAAVEPGAAAAPGVADGGTQTLSQYAQLR
                     WERVKYGDLGSVPIVVGLIAIVITFAILNDTFLTTRNFTNLLLQMSWIATIAIGVVFV
                     LLIAEIDLSVGFVAGVGGVVTVLLLRPDNPGWPWWAAIFVALLCTTTIGFLQGVIVTK
                     AGVPSFVVTLAGFLIWSGVVLILTTSASKVGTIRIQNDVVNGIANDFLSPLAGWILAI
                     AVVILYALSQLRETFTRRARGLARKPPVLTALEIGGLALITLGAVYFLNEDRGVPQVA
                     LILIILLVFWSFVASRLRFGRHIYAIGGSAEAARRAGINVDRVKIMVFMISGLMAGVG
                     GIMLASRLRSVSSAFGGGNTLLLVIAAAVIGGTSLFGGQGRVVSALLGALVITSIENG
                     MNLLGLASGTRFVITGLVLLAAVLVDAYAKRRRTARGVA"
     gene            complement(4889..5668)
                     /locus_tag="AC150V_10485"
     CDS             complement(4889..5668)
                     /locus_tag="AC150V_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR3763385.1"
                     /translation="MADTPLLQCTGISKAFAQVQALYHVDFEVKAGEVMALVGDNGAG
                     KSTLIKGIAGIYPFDEGKVFFEGKETHIHGPRDAAKLGIEIVYQDLALADNLDVVANM
                     VLGREETRNGFVLDETRMEHASQKTLAELSVTTLQSVRNAVGGLSGGQRQAVAVAKAV
                     MWESKLVILDEPTAALGVAQTRQVLDLVRRLAERGLGVVVISHNLHDIFEVSDRITVL
                     RLGQRVALLQRAETTQQEVVEYITAGKLDRVPGMDDGVVEA"
     gene            complement(5755..>5947)
                     /locus_tag="AC150V_10490"
     CDS             complement(5755..>5947)
                     /locus_tag="AC150V_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763386.1"
                     /translation="INSEDGKPTDDPEGDGIVPYIALEPIGVTTENMTDTVIADGFRT
                     VEEICEGDVADTQWCQDNA"
BASE COUNT          931 a         2064 c         2012 g          940 t
ORIGIN      
        1 gctcgcagct acgcacgcgc cgagccgtcg aagtagcgcc tcgcgagcgc ccaggcatcc
       61 ttgccgcaag tcgcccccat gatgcgtccc tcgagatcgt cggcgcgcac gtggatgccg
      121 ccgtggagcc gcgagatgcc cgcctcgtcg gcggcgtcga agtaggtggc ccactgcaac
      181 gtgacgtcct ccgccgggcc ctgctcgtgg agcagctttc ctgcaggcac ggtccattcc
      241 aggagacccc ccgggaacgc cggatcaccg gtgaaggcgc tgagcacttc cgctccagcg
      301 cggctgaacg tgctgtgacc cgagacgtag ccggcgaacg ccggggtgac gaacgtcggc
      361 cgctggtagg gcacccaatc cactgcccga atccagccga cgccggctcg ctcggacgtc
      421 ggatcttccg gattcccgcg ccagcctcgc accgcgatct cgccgactcg gaggccctga
      481 tggcgcccac ctctcgccct cgacttctcc gataccacct cgatcagacc cggcacgagc
      541 ggcaggccgc gtgcgtggta ggacgccgcg gacggatcgc tcgactgccc cttctcgccc
      601 atgtagcgga tcatcgagat cggccgcacg tagtcgtaga aggccttcgc cccccaggcg
      661 gacacggccg cgtcgtggag ggcaccgttg agcgcgaagt acagcttgac gtcccattcc
      721 aaccggtcga cgcgctcgcc ctctcccccg atccgcgccg agatctccgg cgagtcggag
      781 acggcgttcg cgagcgtgtt ccagtgaccc ggcggcgtct ccgagtcggg gccgtcggcc
      841 cagaactcgg cgatcacacg cccgaagtcg gcgcgcagcg tcgggttggc ctcgtacggc
      901 tcgcccgtcc ggacgttctt cggatgcccc gtcccgtcgt tcgtgccgag tcggttgttc
      961 ccgcggctgc ccggaccgat gtcgatgacg tccccgtcgt ccgggtcgag catgctgctg
     1021 aagcgcagga cttcgacggc ggcggctttg tactcggcat cggtcgcggc ctcaccgaga
     1081 gccggcggcg ggccggggtc gatcggcagg ccttcaggag agcgcggcag cgcgaagccg
     1141 gtgacgtgcc cccactgcga gccgacgaac tgctgcacct gggcgggcac gacgaggccg
     1201 ttctgcgtga tctgctgtgc aagcgcgagc ggctgccagc ggttcgggtc gcgcagcgcg
     1261 gcgcccgcct tcgccaccac gagggggctg ttgacgggtc ggtaggcggg atcggcgtat
     1321 cgctgggctt cgttcgagcc gtcatcgcgt ccctgctcga tgacagcggc tgcgatgcgg
     1381 ttcccgagcg cggccggcga atctccctcc cgggaggtgt agtcgattcg atagcagagc
     1441 gattccatcg tcgtgacgag ctgcgagaag gtctcctcga gcccggtcgc aagcgagtag
     1501 cggtgcagca ggacccggta cgccgcgtag ctcagcgccg cctcgcgggc ggcccggacg
     1561 tcttccgcct tgtgcttctc gtggacgaag tagccgtcgg cgatgtcgtc gtaggcggcc
     1621 caggcgtccc acatcgccgc cgacgtgtgg aacaggtttc gggcatgcac cgtcggggcc
     1681 gggaagtcgc gacgaattgc gtcgaggagc gcctcgtccc agacgcgcgc gaccgatctc
     1741 gcgtctgcct tgcgggtgca gccctcgatc aggtacgagt cgctttcgcc gcccgagcag
     1801 ccgccggcga tcgcgagcgc ggcgacggcg agcacgacga gacccctccg cctcattcgg
     1861 gtttctccac cacgagcaac tggtcggagg gcaccatgtc caggcttgtc tcggtgccgt
     1921 cgggccagcg aaccgtcagc cgggagactt cgcgcgcctc gccgagcccg aagttgaggc
     1981 gaggatcctc ggacgacaga tagctgctgc cggcctgcag ctcgcgcacc agcttgcgac
     2041 cgttcggaag gacagcggtg acccgtgccc ccgggtagaa gcccgccagc ccgacctcga
     2101 gccagtgccc tgcggggccg gtgttctcca gcagcacgag cgggctcgcg accgagttga
     2161 ccgcgatatc gaggtcaccg tcgttgtcgt agtccgcagc cgcgctgccg cgcccgtgca
     2221 ggaggccgag ggcgtcgagc ccggtttccg tgccccagtc cgcgaaccgg ccaactctcc
     2281 cttgcgctgt caggttctcg aacgcgagcg gcgactgcga gtccgcgagg agatcggtca
     2341 gcggcacgtg gccgttgaag agcaccacgt ccaggtcggt gtccaggtcg agatccgccc
     2401 acgagacgcc ccagccggtg gaatcgccga ggggcgggcc gaacacctcg cgctcgtccg
     2461 tgaacgtggg gccggtaccg cgcggctcgg cgctgcgaaa gacggcgtgc gtctggttgc
     2521 gaaggttcgt gacgaagagg tcgatgccgc cgtcaccgtc atagtcgccg gctgctaccc
     2581 ccatgccggc gttggggtca gcgacaccgg cccgcgcggc gcgttcctcg aagcggaaac
     2641 ccagccccgc cgggtcggcc tctaccccgc ccggccacgg cacgttcgtg tacaggcggt
     2701 tggggttcgt gtcgttggct acgtagagat cgagatcgcc gtcgagatcg aagtccgaga
     2761 agagcgagcc gagcccgtat tcgaagccag tcgcctcgag tccggcctcc acgccgacct
     2821 cgacgaacga gacccgcccg ccgtcgcgcc cgccgtcgct caggtagagc aggtcacgga
     2881 caccgaggac ggtgttggga aagccaccgt tcgcgtcggg tttgcggttg ttcggatcgg
     2941 cgtagccggc gacgaagaga tcgggccagc cgtctcggtt cacatcgccg accgatgcac
     3001 cgctgtgcca gccgtacgcc tcgacgcccg cgtcgcgggc gccctcgcgg accgtgccgt
     3061 ctccctcgtt ccagaggagc cggttctcac gggccgtcgt gacgaagaga tccgtgttcc
     3121 cgtccaggtc gaggtctgcc gccacgcagc cgtttccacg cacgggatag ctcgcgccgg
     3181 catcgtcgcc gacgctgctg aacgtcccct ccgagttgcg gaagagcgca gtggtcggcg
     3241 gcccgccgtt ctccttccat ttcccgagct cctgctccgc gtacgagttg acgacgaaga
     3301 ggtcgagcca gccgtcgcgg tcgtagtcca gccagcagac gcctcccccc atcatcgccg
     3361 ccgggtccgg agtggtctcc cagcgaaatg cgccgtggcg gaagtcgagg cccgcctgca
     3421 cggcgacgtc cctcaactcg aagccgtcgc tcggcggtct gcttgcagcc cggtcgacgc
     3481 tctcgtcggt gctctccgac gtacaggcgc tgacggccag ggccgccgcg gccagagcca
     3541 gtacgaacct gatcaggact gctgcacgca cgaaaccccg caccgagggt acgtgctcaa
     3601 gcgactccgc gtgccgtacg gcggcgcttc gcgtaggcgt ccacgagtac cgcggcgagc
     3661 agaaccaggc ccgtgatcac gaaccgcgtg ccggacgcga gccccagcag gttcatgccg
     3721 ttctcgatcg aggtgatcac gagcgccccg aggagcgcgg agacgacgcg gccctgaccg
     3781 ccgaagaggc tggtgccgcc gatgacggcg gcggcgatga cgagcagcag cgtgttgccg
     3841 ccaccgaagg cgctcgacac cgagcgcagg cgggacgcga gcatgattcc cccgacaccg
     3901 gccatgaggc cgctgatcat gaagaccatg atcttgacgc ggtcgacgtt gatgcccgcg
     3961 cggcgggcgg cctcggcgct gccgccgatt gcgtagatat ggcggccgaa ccggagtcgc
     4021 gaggcgacga acgaccagaa gacgagcagg atgatgagga tcagtgccac ctgcggcacg
     4081 ccgcggtcct cgttgaggaa gtagacggcg cccagggtta tgagggcgag cccgccgatc
     4141 tccagcgccg tcagtaccgg cggcttgcgg gcgagcccgc gcgcccgccg cgtgaacgtc
     4201 tccctcagct gcgaaagcgc gtagaggatg acgacggcga tcgcgaggat ccagccggcg
     4261 aggggactga ggaagtcgtt cgcgatcccg ttcacgacgt cgttctggat ccggatcgtc
     4321 cccaccttcg atgcgctcgt cgtcaggatc aggacgacac cggaccagat gaggaacccg
     4381 gcaagcgtca cgacgaacga gggcaccccg gctttcgtga cgatcactcc ctgcaggaag
     4441 ccgatcgtcg tggtgcacag gagtgccacg aagatcgcgg cccaccacgg ccagcccggg
     4501 ttgtcgggtc gaaggaggag aaccgtgacg acgccgccta cgccggcaac gaatccgacc
     4561 gagagatcga tctcggcgat caggaggacg aacacgaccc cgatcgcaat cgtcgcgatc
     4621 cacgacatct ggagcagcag gttcgtgaag ttccgagtcg tgaggaaggt gtcgttgagg
     4681 atcgcgaagg tgatcacgat cgcgatcagc ccgacgacga tcggcaccga gccgagatcc
     4741 ccgtacttga cgcgctccca gcgcagctgg gcgtattgcg acagcgtctg cgtgccgccg
     4801 tctgcgaccc caggggcagc ggccgcgccc ggctccacgg cggcggtgtc ggggctctcc
     4861 ggagccgtgg gctctccgtt ctcctgcgtc acgcttccac cacgccgtcg tccatgccgg
     4921 gaacacggtc gagcttgccc gccgtgatgt actcgaccac ttcctgctgc gtcgtctcag
     4981 cgcgctggag cagggcgact cgctgtccga gccgcagcac ggtgatgcgg tccgagacct
     5041 cgaagatgtc gtgcaggttg tgcgagatca ccacgacccc gaggccgcgc tctgcgagtc
     5101 ggcgcacgag gtcgagcacc tgcctggtct gtgcgacgcc gagagcggcc gtcggctcat
     5161 cgaggatgac gagcttcgac tcccacatga cggccttggc gaccgcgacg gcctgccgct
     5221 gcccgcccga gagcccaccg accgcattcc tcacggattg cagcgtcgtg acggacagct
     5281 ccgccagcgt cttctgcgag gcatgctcca tcctcgtctc gtcgagcacg aacccgttgc
     5341 gggtctcctc gcgaccgagg accatgttgg ccacgacgtc gaggttgtcc gcgagcgcga
     5401 ggtcttggta cacgatctcg atcccgagct tggccgcgtc gcgggggccg tgaatgtggg
     5461 tctccttgcc ctcgaagaag accttgccct cgtcgaaggg atagatcccg gcgatgccct
     5521 tgatgagcgt cgacttgccg gcgccgttgt caccgacgag cgccatcacc tcgcccgcct
     5581 tgacctcgaa gtcgacgtgg taaagcgctt gcacctgcgc gaacgccttc gagatccccg
     5641 tgcactggag tagtggtgtg tcggccatcg ccccgtttcg gagagtcaga cgccgtgagg
     5701 gcgggggcgc ccaagtgggc gcccccgctc cggtgttgga tcacagatca acgatcaggc
     5761 gttgtcctgg caccactgtg tgtctgccac gtcgccctcg cagatctcct cgactgttcg
     5821 gaaaccgtcg gcgatgacgg tgtcggtcat gttctcggtc gtcacaccga tcggctcgag
     5881 cgcgatgtac ggcacgatgc cgtcaccctc ggggtcgtcg gtcggcttcc cgtcctcgga
     5941 attgatg
//
LOCUS       DBNGNK010000353         5813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_703855,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000353 DBNGNK010000000
VERSION     DBNGNK010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_703855"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..884
                     /locus_tag="AC150V_10495"
     CDS             99..884
                     /locus_tag="AC150V_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3763387.1"
                     /translation="MSTRLTYHGAASFEIVGPTHRILIDPFLSQNPLAPCAPDELETP
                     DVILVSHAAFDHYGDTPAIAKRTGAPVVCDAAVRAMLIDEGVSPDQVSATTWGIVVEA
                     AGLAIRPVECHHWSSGTLSDGRQVVGNPMAFIIETEPGVRIYHYGDTCIFDMRLIGEL
                     YRPTIGLLGCTLPRELSHMIPGPATFLTGEMDADEAARVAEMLGLDLAVACHYLAKDP
                     EVERFLELVPVHDSTGKRTALAPEVGETVVVEGGTYAIEGGSG"
     gene            881..1723
                     /locus_tag="AC150V_10500"
     CDS             881..1723
                     /locus_tag="AC150V_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3763388.1"
                     /translation="MRLVTYESGGRVSVGVRRDGDIVHAGYEDMLDLIADGPGGLDRA
                     AAAAASGPPVDGAKVLAPLRPGKVLCAGINYRSHGAEAGAAPQEPYFFAKLPNAVCGP
                     GDPIVVPTPETFTDWEVEFAFVIGTRAKHVSAASAMEHVYGYTLLHDVSARDVQFDWS
                     KRTQDSQITLGKNPDTFSPIGPELVTKDELPDPGNVRLATYVNGEQKQDGTTADWLFP
                     IPVLIEFLTQLVTLEPGDLVSTGTPAGVGYFLDPPERLQPGDEVTIEAEGIGRLTNPV
                     VAGW"
     gene            1741..2820
                     /locus_tag="AC150V_10505"
     CDS             1741..2820
                     /locus_tag="AC150V_10505"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3763389.1"
                     /translation="MDERIRVGIGSDLIAPDGSSLMAAALAEHFLPVDGLEWETFGEP
                     RAELAPDDVGDFDAVITLWQRFTPATFEGVERLATIARWGVGYDMVDVEACTDNDVLV
                     SITRDAVRRPVAEAILTYVLVLAKRVLSNDRLVRTARWDEKGDVLGLGLRERVLGTIG
                     VGNIGAELLGLVQPLGLARVLAVDPYVSGDTAAELGVELTDLDTLLAESDFVCVNCPL
                     TPDTRHLIGAAELRRMKPTAFLINTARGPIVDEPALVDALRRGQIGGAALDVFEQEPL
                     PADSPLIALDNVVLAPHAIAWTDELFALNGSGACANALAVLRGGVPAHVVNRDVIGRP
                     GFQKKLERLRGRWEMNNRSGREHDG"
     gene            2813..3679
                     /gene="panB"
                     /locus_tag="AC150V_10510"
     CDS             2813..3679
                     /gene="panB"
                     /locus_tag="AC150V_10510"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR3763390.1"
                     /translation="MADEPKKLTIFDLQAKAAAGEKVFQVTAVDYPTAQLVDRAGIEC
                     ILIGDSLGMTALGYASTVPVTMEEMVYHAKAISRAAKRAILVGDLPLGAYHASTADAV
                     DNAMRMVKEGGADVVKMEGGEDIVPMVEAVITAGIPVMGHIGLTPQLMSKLGGFRVQG
                     KSGAAGAQLVRDAVALERAGCFATVLEAIPDRVAALITERLEIPTIGIGAGPDCDGQN
                     LVLHDLVGLFDRFLPRFVKRYANVGEQIDQALQDFQAEVRDGAFPTAEHSFVMDDEEY
                     EQLLAEVSERVA"
     gene            3669..4019
                     /locus_tag="AC150V_10515"
     CDS             3669..4019
                     /locus_tag="AC150V_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3763391.1"
                     /translation="MSPERATVVGLRGAEPLELVPGVTMRPLFGEGAMLNLLEVEPGA
                     LVPAHDHPHEQLGYVLEGELVLEIDGVEHTLRAGDAYQIPGGTTHAAWSSGPCLVLDI
                     FQPVREDYRERAGG"
     gene            4016..4807
                     /locus_tag="AC150V_10520"
     CDS             4016..4807
                     /locus_tag="AC150V_10520"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR3763392.1"
                     /translation="MRANPVKEALARGDTVLGTMVTEFTTPGIARLAAAAGAEFVLLD
                     MEHTGYEYERMRGVIAAAEASDIVTLLRVPDSDYQFISRGLDIGAMGVMLASVGSTAE
                     AKAIAAAARFPPAGRRGFGLLLRRDEFEPEGLPATLAKVNESTMTLAQIETTAGLDAV
                     EEIAAVDGIDALWIGHFDLTASMGIPGEFTSDRFLAAVDRVIGAGRAHGKPAGIVCAS
                     VEEGADFLARGFRLLAFSIDILLYQDALAAGLAGLADARDGPRPG"
     gene            4804..>5813
                     /locus_tag="AC150V_10525"
     CDS             4804..>5813
                     /locus_tag="AC150V_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010588139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3763393.1"
                     /translation="MSVLRAGITRDIVGADGRLVFDLDALESAPGVETTLVSDADELT
                     AGDVADLDALVLFKPRVSAATLDGLGRLLLVARLGVGVDNVDVEACSASGVLVTTTPD
                     GVRRPMAAGAMAFLLALAHRLPEMDRHVREGGWQRFAHIGTGLEGRTLGIVGLGNIGR
                     DVARLAVPFGLRLVAADPYLASPVEGVELLELDELLATSDFVVVAAPLTPETHHLIDA
                     GRLARLRPSAYLINICRGPIVDQQALTEALREQRIAGAGLDVFETEPIEPGDALLSLD
                     NVILAPHAVGLTDEIFRLSGRSVSNSVLAVSRGEVPEHVLDRRALEHERVRELLAAQA
                     AAT"
BASE COUNT          888 a         1997 c         2047 g          881 t
ORIGIN      
        1 tcaaggccgc cgacgagcac ggggtaccgg ttccgatcaa ccgagctttc gtccagctcg
       61 tcaagggctg ggagtcgatg cgagggctcg gtggctaggt gagcacgcgg ctgacctacc
      121 acggcgctgc cagcttcgag atcgtcgggc cgacgcaccg gatcctgatc gacccttttc
      181 tgtcgcagaa cccgctggcg ccgtgcgcgc ccgacgagct cgagacgccc gacgtgatcc
      241 tcgtcagcca cgcggccttc gaccactacg gtgacacgcc cgcgatcgcc aagcgcacgg
      301 gtgcccctgt cgtctgcgac gcggcggtcc gagcgatgct gatcgacgag ggcgtctcgc
      361 ccgaccaggt cagcgccacg acctggggga tcgtcgtcga ggcggcgggt ctcgcgatcc
      421 gccccgtcga gtgccaccac tggtcgtcgg gaacgctctc ggacggacgg caggtcgtgg
      481 gaaacccgat ggccttcatc atcgagacgg agcccggtgt tcgcatctac cactacggcg
      541 acacgtgcat cttcgacatg cgcctgatcg gcgagctgta tcggccgacg atcggcctgc
      601 tcggctgcac gctgccgcgg gagctctcgc acatgattcc gggcccggcc acgttcctga
      661 ccggcgagat ggacgcggac gaggccgccc gcgtggccga gatgctcggg ctcgatctcg
      721 ccgtcgcctg ccactacctc gccaaggacc ccgaggtcga gcgctttctc gagctcgtgc
      781 ccgtgcacga ctcgaccggg aagcggacag ccctcgcgcc ggaagtcggc gagaccgtgg
      841 tcgtcgaagg aggcacgtac gcgatcgagg gcgggagcgg atgaggctcg tcacctacga
      901 gagcggcggt cgcgtgtcgg tcggagtccg ccgggacgga gacatcgtcc acgccggcta
      961 cgaggacatg ctcgacctga tcgcggacgg gcccggtggg ctggatcgtg ccgccgccgc
     1021 ggcggcgagc ggcccgccgg tcgacggtgc gaaggtgctc gcgccgctgc gacccggaaa
     1081 ggtcctctgc gcaggcatca actaccggag ccacggcgcg gaggcggggg cggcgccgca
     1141 agagccgtac ttcttcgcga agctcccgaa cgcggtgtgc gggccgggcg atccaatcgt
     1201 cgtcccgacg cccgagacct tcacggactg ggaggtcgag ttcgcgttcg tgatcgggac
     1261 gagggcgaag cacgtctcgg ccgcctctgc gatggagcac gtgtacggct acacgctgtt
     1321 gcacgacgtc agcgcccgcg acgtccagtt cgactggtcg aagcgcacac aggattccca
     1381 gatcactctc ggcaagaacc cggatacctt ttcgccgatc gggcccgagc tggtgaccaa
     1441 ggacgagctg ccggatccgg gcaacgtgcg gcttgcgaca tacgtgaacg gcgagcagaa
     1501 gcaggacggg acgaccgccg actggttgtt cccgatcccc gtcctgatcg agttcctgac
     1561 acagcttgtc accctcgagc cgggcgatct cgtgtcgact ggaacgcccg ccggagtcgg
     1621 ctacttcctc gaccctcccg agcgcctgca gccgggcgac gaggtgacca tcgaagccga
     1681 gggaatcggc cggctgacga accccgtcgt cgcgggctgg taggacgcca cgctcgggcc
     1741 gtggacgagc ggatccgtgt cggcatcggc agcgatctca tcgcacccga cggctcctcg
     1801 ttgatggccg ccgcgctcgc ggagcacttc ctaccggtcg acggcctcga gtgggagacg
     1861 ttcggcgagc cgcgggctga gctcgcgccc gacgatgtcg gcgacttcga cgccgtgatc
     1921 acgctctggc agcggttcac gccggcgacg ttcgaaggcg tcgagcggct ggcgacgatc
     1981 gctcgctggg gcgtgggtta cgacatggtg gacgtcgagg cctgcaccga caacgacgtg
     2041 ctcgtctcga tcacccgcga cgcggtccgc cggccggttg ccgaggcgat cctgacctac
     2101 gtcctcgtcc tcgccaagcg ggtcctttcg aacgaccggc tcgtccgcac ggcgcgttgg
     2161 gacgagaagg gagacgtgct cggcctcggg ctccgggaac gtgtgctcgg aacgatcggc
     2221 gtcgggaaca tcggcgcgga gctcctcggc ctcgtccaac cgctcggcct ggctcgcgtg
     2281 ctcgccgtcg acccgtacgt ctccggcgac acagccgccg agctcggtgt cgagctcacc
     2341 gacctcgaca cgctgctggc ggagtccgac ttcgtctgtg tcaactgccc gctgacgccc
     2401 gacacgcggc atctgatcgg ggcggccgag ctgcgacgga tgaagccgac ggcgttcctg
     2461 atcaacacgg cccgtggccc gatcgttgac gagcctgcgc ttgtcgacgc gctccggagg
     2521 gggcagatcg gaggagcggc gctcgacgtg ttcgagcagg agccgctgcc ggctgactcg
     2581 ccgttgatcg cgctcgacaa cgtcgtcctc gcgccgcacg cgatcgcgtg gacggacgag
     2641 ctgttcgcgc tcaacggcag cggcgcctgc gcgaacgcgc tcgccgtact tcgcggcggg
     2701 gttcccgcac acgtggtcaa ccgcgacgtg atcgggcgac ccgggttcca gaagaagctc
     2761 gagcgcctgc gcggtcgctg ggagatgaac aaccgatcag ggagggagca cgatggctga
     2821 cgaaccgaag aagctcacga tcttcgacct gcaggccaag gccgccgccg gcgagaaggt
     2881 cttccaagtc acagccgtcg actacccgac ggctcagctc gtcgaccgag cgggcatcga
     2941 atgcatcctc atcggcgact cgctcgggat gacagccctc ggctatgcga gcaccgttcc
     3001 cgtcacgatg gaagagatgg tctatcacgc gaaggccatc agtcgggcgg cgaagcgggc
     3061 gattctcgtt ggtgacctcc ctctcggcgc ataccacgcg agcacggccg acgcggtcga
     3121 caacgcgatg cggatggtca aggagggcgg cgccgacgtc gtgaagatgg agggcggcga
     3181 agacatcgtc ccgatggtcg aggcggtcat caccgccggg atccccgtca tgggccacat
     3241 cggcttgacg ccgcagctga tgtcgaagct cggcggcttc cgcgtccagg gcaagagcgg
     3301 cgccgccggg gcccagctcg tgcgtgacgc cgtcgcactc gagcgcgccg gttgcttcgc
     3361 gaccgtgctg gaggcgattc ccgaccgagt cgcggcgctg atcacggagc ggctcgagat
     3421 cccgacgatc ggaattggcg ccgggcccga ctgtgacggt cagaacctcg tcctgcacga
     3481 tctcgtcggc ctcttcgacc gctttctgcc gcgtttcgtg aagcgctacg cgaacgtcgg
     3541 cgagcagatc gaccaggcgc tgcaggactt ccaggcggag gtgcgggacg gcgctttccc
     3601 gactgcagag cacagcttcg tgatggacga cgaggagtac gagcagctgc tggcggaggt
     3661 gagcgagcgt gtcgcctgag cgagcgaccg tcgtcgggct gcgcggcgcc gagccgctgg
     3721 agctcgtgcc cggcgtgacc atgcgtcctc tcttcggcga gggcgcgatg ctcaacctgc
     3781 tcgaggtgga gccgggcgcc cttgtgcccg cgcacgacca cccgcacgag cagctcggct
     3841 acgtcctcga gggcgagctc gtgctggaga tcgacggcgt cgagcacacc ctccgggcgg
     3901 gcgacgcgta ccagatccca ggcggcacga cgcacgcggc ctggtcgagt ggcccctgcc
     3961 tcgtgctcga catcttccaa cccgtgcgcg aggactaccg ggagcgcgcc ggcggatgag
     4021 ggcgaacccg gtcaaggagg cgctcgcccg cggcgacacc gttctcggga cgatggtcac
     4081 ggaattcacg actccgggga tcgcacggct ggccgcagcc gcgggcgccg agttcgttct
     4141 cctcgacatg gagcacacgg gctacgagta cgagcggatg cggggcgtca tcgcagccgc
     4201 agaggcaagc gacatcgtca cgctactgcg tgttcccgac tccgactacc agttcatctc
     4261 gcgcgggctc gacatcggtg cgatgggcgt gatgctcgcg tccgtcggca gtacggccga
     4321 ggccaaggca atcgccgccg cggctcgctt tccgccggcg ggaagacgcg gcttcgggct
     4381 cctgctgcgg cgcgacgagt tcgagcccga gggactgccg gcgacgcttg ccaaggtgaa
     4441 cgagtcgacc atgacgctcg cccagatcga gacgacggcc gggctcgacg cggtggagga
     4501 gatcgcagcc gtcgacggga tcgacgcgct ctggatcggg catttcgacc tcacggcgtc
     4561 gatgggcata ccgggggaat tcacgagcga ccgcttcctg gccgccgtcg accgtgtgat
     4621 cggggccggc cgggcgcacg gcaagcctgc cggcatcgtc tgcgccagcg tcgaggaagg
     4681 cgcagatttc ctggccaggg gcttccggct gctcgccttc tcgatcgaca tcctcctcta
     4741 ccaggacgcg ctggcagcgg ggctggccgg actcgcggac gcacgcgacg ggccgcgccc
     4801 ggggtgagcg tgctccgggc aggcatcact cgggacatcg tcggcgcaga cgggcgtctc
     4861 gtcttcgatc tcgacgcgct cgagtccgcg cccggcgtcg agacgacgct tgtcagcgac
     4921 gcggacgaac tcactgcggg cgacgtcgct gacctcgacg cgctcgtcct gttcaagccg
     4981 cgcgtgtcgg ctgcgaccct cgacggtctc gggcgcctgc tgctcgtcgc acggctcggg
     5041 gtcggcgtcg acaacgtcga cgtcgaggcc tgctccgcga gcggcgtgct cgtcacgacg
     5101 acgcccgacg gcgtccgccg gccgatggcc gctggagcca tggcctttct gctcgctctg
     5161 gcgcaccggc tcccggagat ggaccgtcac gtccgagaag gtggctggca gcgcttcgcg
     5221 cacatcggca cggggctgga gggacggacg ctggggatcg tcggcctcgg caacatcgga
     5281 cgcgacgtcg cgcgcctggc cgtgccgttc gggctgcgcc tggtggcggc cgatccctac
     5341 ctggcgagcc ccgtggaggg cgtcgagctc ctagagctcg acgagctgtt ggctacctcc
     5401 gacttcgtcg tcgtcgccgc gccgctcaca cccgagacgc accacttgat cgacgccggg
     5461 cggctggctc gactccggcc gagcgcgtac ctgatcaaca tctgccgcgg gccgatcgtc
     5521 gaccagcagg cacttaccga ggcgctgcgc gagcagcgga tcgcgggcgc agggctggac
     5581 gtgttcgaga cagagccgat cgagcccggc gacgcgctcc tctcgctcga caacgtcatc
     5641 ctcgcgccgc acgccgtcgg cctcacggac gagatcttcc ggctctcggg tcgaagcgtc
     5701 tcgaacagcg tcctggccgt gtcccggggc gaggttccgg agcacgtgct cgaccgccgc
     5761 gcgctcgaac acgaacgcgt gcgcgagctt ctggcggccc aggccgccgc gac
//
LOCUS       DBNGNK010000354         5310 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_346452,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000354 DBNGNK010000000
VERSION     DBNGNK010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_346452"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="AC150V_10530"
     CDS             <1..535
                     /locus_tag="AC150V_10530"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--NADP reductase domain-containing
                     protein"
                     /protein_id="HIR3763394.1"
                     /translation="DGRVEEVEVARNELVASADGRIRAVQTDERETIPCGIVFRSIGY
                     HGLAIPGVPFDEGWGIIPNDAGRVIGEDGRPLPGLYCAGWIKRGPTGVIGTNKKDATE
                     TVEALLADAAAGTLRRQDEASAERVDDLLAERGIEVVSYAGWESIDAVERSRGEGQGR
                     PRVKLCTWDELLAAARR"
     gene            597..1922
                     /locus_tag="AC150V_10535"
     CDS             597..1922
                     /locus_tag="AC150V_10535"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012923158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR3763395.1"
                     /translation="MSEVTREQLWGGETEKAVANFPVSGEPIPIAVARWLARIKAAGA
                     RVNGELGLLDRDKADRIAASGDRVAAGEFDDQFPIDVFQTGSGTSSNMNANEVIAALA
                     GDDVHANDDVNMGQSSNDVFPSAVHLAALSEITHDLLPALELLAASLERKSFDFDDIV
                     KSGRTHLMDAVPVTLGQEFAGYAAQVRQGIARVDDSLPRLGQIPLGGTATGSGLNTHP
                     EFAARVRDLLSRETGLTIGAPVDHFEAQAARDGLVEASGALKVVAVSLTKVANDLRLM
                     GSGPRAGLSELLLPELQKGSSIMPGKVNPVIPEVVTQVGAQVIGNDAAITVGGLQGHF
                     ELNVFIPLMARNLLESIKLLAAASRLLSEKCVDGIEANREQCERYAELTLSAATALNP
                     YIGYDKATEIVKEAASSGRSLREVAREAGVDESVLDKALDYAAMAKPHA"
     gene            complement(1944..2135)
                     /locus_tag="AC150V_10540"
     CDS             complement(1944..2135)
                     /locus_tag="AC150V_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763396.1"
                     /translation="MAFKDKVRGWLKRDAGDKKGAEEESDDLARLEQKLDTQAGPSSY
                     ARPGNIDFEADQKPPKRGY"
     gene            complement(2168..3088)
                     /gene="lipA"
                     /locus_tag="AC150V_10545"
     CDS             complement(2168..3088)
                     /gene="lipA"
                     /locus_tag="AC150V_10545"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR3763397.1"
                     /translation="MQTSLPVADRPRRPDWMKVRAPSADGRYYDVKKLIQGAGLNTVC
                     EEARCPNIGECWGRGTATFQILGDTCTRACRYCYVHSGTPEAPPDPLEPLRLAQTAKL
                     MGLHHVVVTSVDRDDLPDRGAGHFAATVRALKAKLPLCSVEVLTPDFLGVEEAALGTV
                     LDARPEVFNHNIETVRRLHRRMRGAKASYDTALWLLARAKEIADYPVLTKSGIIVGLG
                     ETNEEVVETMRDLRANGVDVVTIGQYLQPSQKHAKIDRWVHPDEFRWLREQGEALGFG
                     SVFAGPLVRSSYRADEQRHAAETGRGAVAY"
     gene            complement(3136..3846)
                     /gene="lipB"
                     /locus_tag="AC150V_10550"
     CDS             complement(3136..3846)
                     /gene="lipB"
                     /locus_tag="AC150V_10550"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR3763398.1"
                     /translation="MSVGAYLLDLGVTPYGEAVAVQRSLAAAVSQRAIPETVIMLEHP
                     PVITLGRRTDEVELHVPDGVEVEIAETDRGGKSTFHGPGQLVCYPILDLNNHGRDVKQ
                     YVRDLEEAVIRALAAFELRGQRLEGLTGVWLTDPPRKIASIGVHVSRWVTTHGYALYV
                     ALDPAPFTDWITACGLDEARFTTMGLELGRTVTVADVRPAAAASLAEVFGLALEPLPL
                     DAGLGLWPEPVHEKLAAR"
     gene            complement(3843..4523)
                     /locus_tag="AC150V_10555"
     CDS             complement(3843..4523)
                     /locus_tag="AC150V_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006307 - DNA alkylation repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR3763399.1"
                     /translation="MGRRGLRRALRAHAEPLRDAEYAAGAQRYMKSSMPYLGVRMPDL
                     RRVWRELFREYPLDDREAFQSALHDLWDEADYREERYTALALLERYPVYVTLPLLERL
                     IVEGAWWDLVDTLARRVGELLRTDSSVAAEMRRWSHDRNMWKRRVSIICQLGFKEQTD
                     LDLLYACIEANVEDREFFIRKAIGWALRDYAWHDPREVDRYVRLHADTLSPLSHREAS
                     KNLARLLV"
     gene            complement(4505..>5310)
                     /locus_tag="AC150V_10560"
     CDS             complement(4505..>5310)
                     /locus_tag="AC150V_10560"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR3763400.1"
                     /translation="AAAPPAAAPAPPAEVPPPTPPKPAAADTEAGPGETVEPMTAMRR
                     GVMEHMRRSVDTSAHVTSGIEVDMSRIVAVRRKLKPEYEQSYSVNPTYLAFIARATVD
                     TLAEWPWINGEIRGETIVTRQFVNLGIAVALQDGKGLIVPVIRNAEGLNLLGMARAIA
                     DLAERARTKKLLPDDVQGGTFTITNPGGFGTFHGTPIINQPQAGILGTYAVVKRPWVV
                     QDELGADVIAIRPMMNLTLTYDHRLVDGAYAGAFLRDLRERLETWEDVG"
BASE COUNT          815 a         1787 c         1872 g          836 t
ORIGIN      
        1 cgacggccgt gtcgaggagg tcgaggttgc acgcaacgag ctcgtcgcgt ccgccgacgg
       61 acggatccgc gccgtccaga ccgacgagcg ggagaccatc ccgtgcggca tcgtctttcg
      121 cagcatcggc taccacgggc tcgcgattcc aggcgtgccg ttcgacgagg gctgggggat
      181 catccccaac gacgccggcc gcgtgatcgg cgaggacggc cggcccctgc ccggcctcta
      241 ctgcgccggc tggatcaagc gcggcccgac gggtgtgatc ggcacgaaca agaaggacgc
      301 gacggagacc gtcgaagcgc tgctcgccga cgcggcggcg ggaacgttgc gccggcagga
      361 cgaagcgagt gccgagcgcg tcgacgacct gctcgccgag cgcggcatcg aggtggtgtc
      421 gtacgccggc tgggagtcga tcgacgccgt cgagcgatcg cggggcgagg ggcaggggag
      481 gccgcgagtg aagctctgca cgtgggacga gctgctcgct gcggcccgcc gctagccaca
      541 gcggcggacg ctcgcacggc gccttcgcag atcgattgcc cgatagactg gggtctgtga
      601 gcgaagtcac gcgcgagcaa ctctgggggg gcgagaccga gaaagccgtt gcgaacttcc
      661 ctgtgtcggg tgagccgatc ccgatcgccg tcgcgcgttg gctggcgcgg atcaaggcgg
      721 ctggagcgcg ggtgaacgga gagctcgggc tgctcgaccg cgacaaggcc gacaggatcg
      781 cagcgtcggg cgaccgggtc gcggccggag agttcgacga tcagttcccg atcgacgtct
      841 tccagactgg ctcgggcacg tcctcgaaca tgaatgcgaa cgaggtgatc gcggcactcg
      901 ccggcgacga cgttcatgcg aacgacgacg tcaacatggg ccagtcgtcc aacgacgtct
      961 ttccgtctgc agtccatctt gccgcgctca gcgagatcac ccacgacctc ctgccggccc
     1021 tggagctcct cgccgcctcg ctcgagcgaa agtccttcga cttcgacgac atcgtcaagt
     1081 ccggtcgcac ccacttgatg gacgccgttc cggtgaccct cggacaggag ttcgccggct
     1141 acgcggcgca ggtgcgccag gggatcgcac gggtcgacga ctccctgcca cgcctcggcc
     1201 agatccccct cggcggcacg gcgacgggaa gcggcctgaa cacccacccc gagttcgcgg
     1261 cgcgggtacg cgacctgctc tcgcgtgaga caggactgac catcggagcg ccggtcgacc
     1321 acttcgaggc acaggccgcc cgagacggcc tcgtcgaggc atcgggcgcg ctgaaggtcg
     1381 tcgccgtctc tctgacgaag gtggcgaacg acctgcggct gatggggtcc gggccccggg
     1441 ctgggctctc ggagctcctc ctgcccgagc tgcagaaggg gagttcgatc atgcccggga
     1501 aggtgaaccc ggtgatcccg gaagtcgtga cgcaggtcgg tgcccaggtg atcggcaacg
     1561 acgccgctat caccgtcggc ggcctgcagg ggcacttcga gctgaacgtc ttcatcccgc
     1621 tgatggcccg caacctgctc gagtccatca agctccttgc cgcggcgtca cgcctgctgt
     1681 cggagaagtg cgttgacggg atcgaggcga accgcgagca gtgcgagcgc tacgcggagc
     1741 tcacgctgtc ggcggccacc gccttgaacc cgtacatcgg gtacgacaag gcgaccgaga
     1801 tcgtcaagga ggcggcgtcg agcggccggt cgttgcgcga ggtcgcccgc gaggccggcg
     1861 tcgacgagag cgtgctcgac aaggcgctcg actacgcggc gatggccaag ccgcacgcct
     1921 agccgggagg agctccggcc tttctagtag ccgcgcttcg gcggcttctg atccgcttcg
     1981 aagtcgatgt tgccaggccg tgcgtacgag ctcggtccgg cctgtgtgtc gagcttctgc
     2041 tcgaggcggg cgaggtcgtc cgactcttct tcggcgcctt tcttgtcgcc cgcgtcgcgc
     2101 ttcagccagc cacgcacttt gtccttgaac gccatggggg gaccgtagcg gttcgccctc
     2161 gcggcagcta gtaggcgacg gcgccgcggc ccgtctcggc ggcgtgcctc tgttcgtccg
     2221 cccggtacga ggaccgtacg agcgggcccg cgaagaccga gccgaagccg agcgcctcgc
     2281 cctgctcgcg cagccagcgg aactcgtcgg ggtgaaccca gcggtcgatc ttcgcgtgct
     2341 tctgcgacgg ctgcaggtac tgcccgatcg tgaccacgtc gaccccgttg gcgcggaggt
     2401 cgcgcattgt ctcgacaacc tcctcgttgg tctctcccag accgacgatg atccccgact
     2461 tcgtcaggac cggatagtcg gcgatctcct tggcgcgcgc cagcagccag agggcggtgt
     2521 cgtagctcgc cttagcaccg cgcatccggc gatggaggcg gcgcaccgtc tcgatgttgt
     2581 ggttgaagac ctccggccgc gcgtccagga cggtgccgag cgctgcctcc tcgacgccga
     2641 ggaagtcagg ggtcagaacc tcgaccgagc acagcgggag cttcgccttg agcgcccgca
     2701 cggtcgcggc gaagtggccg gcaccgcggt cgggcaggtc gtcgcggtcg accgacgtca
     2761 cgacgacgtg gtgcaaaccc atcagtttcg ccgtctgcgc gagccggagc ggctctagcg
     2821 gatcgggggg cgcttccgga gtccccgaat ggacgtagca gtagcggcag gcccgtgtac
     2881 aggtgtcgcc caggatctgg aacgtcgcgg tgccgcgtcc ccagcactcg ccgatgttcg
     2941 ggcagcgggc ctcttcgcag acggtgttca gccccgcgcc ctgaatcagc ttcttgacgt
     3001 cgtagtagcg tccgtccgcg ctcggggcgc gcaccttcat ccaatccggc cggcgaggac
     3061 ggtcggcgac gggtagcgag gtctgcatcg acaatcagtg tattggcgcc cggcgccgga
     3121 gcgcctcacc gtgtctcagc gcgcggcgag cttctcgtgc acgggctcgg gccagaggcc
     3181 gagaccggcg tcgaggggga ggggctccaa ggcgagtccg aagacctcgg ccaacgacgc
     3241 ggccgcagcg ggccgcacgt ctgccacggt caccgttcgg cccagttcga gccccatcgt
     3301 cgtgaaacgg gcctcgtcga gcccgcacgc agtgatccag tcggtgaacg gagccgggtc
     3361 gagggcgacg tagagcgcgt aaccgtgcgt cgtgacccag cgggacacgt gcacgccgat
     3421 gctggcgatc ttgcgtgggg gatcggtcag ccagacgccg gtcagcccct cgagccgctg
     3481 gcctcgaagc tcgaacgcgg ccagggcacg gatcacggcc tcctccagat cgcggacgta
     3541 ttgcttgaca tcgcgcccgt ggttgttgag gtcgagaatc ggatagcaga cgagctggcc
     3601 cggcccgtgg aacgtcgact tcccgccgcg gtcggtttcg gcgatctcga cctcgacgcc
     3661 gtcgggcacg tgcagctcga cctcgtcggt gcgccgtccg agcgtgatca cgggtgggtg
     3721 ctcgagcatg atcacggtct ccgggatcgc ccgctgcgaa acggcggcgg cgagagagcg
     3781 ctgcaccgca acggcctcac cgtacggagt cacgccgaga tcgagtagat acgcgccaac
     3841 gctcatacca ggagcctagc caggttcttc gacgcctccc tatggctgag cggcgacaga
     3901 gtgtcggcgt gcaaccgcac gtagcggtcg acctcccgcg ggtcgtgcca cgcgtagtcc
     3961 cggagcgccc agccgatcgc cttgcggatg aagaactcgc gatcctcgac gttcgcctcg
     4021 atacaggcgt agagcaggtc gaggtcggtc tgctccttga acccgagctg gcagatgatc
     4081 gagacacgtc gtttccacat gttccgatcg tggctccagc ggcgcatctc cgccgccacc
     4141 gaggagtcgg tcctgaggag ctccccgacg cggcgcgcga gtgtgtcgac gaggtcccac
     4201 caggcgcctt cgacgatcag gcgctcgagc agcggtagcg tcacgtacac cgggtatcgc
     4261 tccagcagcg cgagcgccgt gtagcgctcc tctcgatagt cggcctcgtc ccacaggtcg
     4321 tgcagcgcgg attggaacgc ctcgcggtcg tcgagcgggt actcgcgaaa gagctctcgc
     4381 cagacgcgtc ggagatccgg catgcggacg ccgagataag gcattgagct cttcatgtac
     4441 cgctgggccc ccgccgcgta ctcggcgtcc cgcagcggct cggcgtgggc gcgaagcgcc
     4501 cttctcagcc cacgtcttcc caggtctcga gccgctcgcg cagatcgcgg aggaaagcgc
     4561 ccgcgtaggc gccgtccacg agccgatggt cgtaggtcag ggtgaggttc atcatcggtc
     4621 gaatggcgat cacgtccgcg ccgagctcgt cctggacgac ccagggccgc ttgacgaccg
     4681 cataggtgcc gaggatcccc gcctgcggct ggttgatgat cggcgtgccg tggaaggtgc
     4741 cgaagccgcc ggggttcgtg atcgtgaagg ttccgccttg gacgtcgtct ggcagcagct
     4801 tcttcgtccg cgcccgctcg gccaggtcgg cgatcgcccg cgccatgccg aggaggttga
     4861 ggccctcggc gttccggatc acggggacga tgaggccctt gccgtcctgc agcgcgacgg
     4921 cgatgccgag attgacgaac tggcgggtca cgatcgtctc gccgcggatc tcgccgttga
     4981 tccagggcca ctcggccagc gtgtcgaccg tggcgcgcgc gatgaacgcg aggtaggtcg
     5041 ggttcaccga gtacgactgc tcgtactcgg gcttgagctt gcgccggacc gcgacgatcc
     5101 tcgacatgtc cacctcgatg ccgctcgtga cgtgggcaga ggtgtcgacc gagcgtcgca
     5161 tgtgctccat cacgccgcga cgcatcgcgg tcatcggctc gaccgtctcg cccgggcccg
     5221 cctcggtgtc ggctgcggcg ggcttcggcg gagtgggcgg cgggacctcg gccggcggcg
     5281 ccggagctgc ggccggcgga gccgcagcgg
//
LOCUS       DBNGNK010000355         4002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_62617,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000355 DBNGNK010000000
VERSION     DBNGNK010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_62617"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="AC150V_10565"
     CDS             <1..667
                     /locus_tag="AC150V_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763401.1"
                     /translation="CDTLVLTPGPWARDLWRLLDLPDELGGEPVFHYWEVQEGDHSQR
                     GATLEETAPVVHLDLHEPLLDREGTLLHPGPWGIYFRPSVSGGVTGGGLPTPLSPDCA
                     LDPYGPSHPDHGVGDPEQAAGFTAGLERALGRFGEDTWRSECFRAPMCFTADSHPLVD
                     WVRPNAYALIDSNHGFKLLALGRLAAAELVGGHAVELDPFRLERFARAAVHPVSASPY
                     PWT"
     gene            complement(664..957)
                     /locus_tag="AC150V_10570"
     CDS             complement(664..957)
                     /locus_tag="AC150V_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763402.1"
                     /translation="MSYRLWWRWNDGVHGHVWLSADDARRLADEMALQGCGALADRIE
                     SAEEELTIRPQEVAEALESISARPIALTDVALWEDWVRFLEGAEAKGGLLVRR"
     gene            complement(954..2060)
                     /locus_tag="AC150V_10575"
     CDS             complement(954..2060)
                     /locus_tag="AC150V_10575"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR3763403.1"
                     /translation="MSKETAFYPRLREMTDDWMDLFGYRAPSVVSDTQEEYRACREAA
                     ALMDFTMLRKVDIDGPGAQAFVNTIVARDVATLAPGQIAYGALCDEDGKMVDDCTVML
                     RSADSIRFCGANDRDYEIFRDKAPQGVEVREVTDATPHLCLQGPQSRAMLQGLTESDL
                     SNAAFPYYTFREDVEIAGVPAFMTRLGYTAELGYELWVERDRALELWDALIEALTPQG
                     MKVIGMVALDLFRIEGGFIIGGVEYDPSVSPYECGLGWSVALDKADVRGREGLLRDRD
                     AAELRLTSVELDGGGDAASGAPLSVDGVEVGLVTQAVEAPCLDGRTLGLAKIRKDLNV
                     PGTVVVARVDGSDVTGRVVEHPVYEKSRRKAKES"
     gene            complement(2095..>4002)
                     /locus_tag="AC150V_10580"
     CDS             complement(2095..>4002)
                     /locus_tag="AC150V_10580"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR3763404.1"
                     /translation="RRRDGVAGTAHLLVFYGFLVLFIGTAILAFQDDVAEPLFGWTFW
                     EGPFYLGYSLFLDVFGFALLVGLVLFAAKRLSGPPRLDYARVDGRPGDYDRRRYVSGD
                     WIFVGSLFFLGATGFLLEALRIAVDDPSFEKWAPLGWLFGQALRGIGVEGDGADTARF
                     GLWWVHGVAALAFVSAIPFTKAVHMLTGPASVATRNERSGRALDPLPDDPSPEEVGVG
                     VVRDLAPTYLLQLDACTKCGKCHAACPAAATGYPLSPRDLVLDLREQSEGTYGARSAM
                     GITPVYEPEAEIVGQTIRPETLWSCMQCMACVEICPVGIEHVPIINQLRRRLVDRGEL
                     DPSLQSTLETIYDSGNSFGEPKRKRGRWAKELEVKDARKEPVELLWFVGDYASLDPRN
                     SGISQTIARILGHAGVDFGILYEAERNAGNDVRRVGEEVLFLDLAENNIEAISGCEFE
                     RILTSDPHSFNTIRNEYPALGGEWDVVHHSQLLLELLESGAIAPAKTLDVRVTYHDPC
                     HLGRYNGVFDAPRNVLAAIGCELVEMPRNRSNSFCCGAGGGRIWMDELKEGDAPRPSE
                     SRIHEAVALREIKYFVTACPKDVTMYEDAIKTSGHEGEIELRELSELVYEALGLGTTP
                     APLGAGEAGGA"
BASE COUNT          599 a         1455 c         1348 g          600 t
ORIGIN      
        1 ctgtgacacg ctcgtcctca cgccgggccc atgggcccgc gacctctggc ggctcctcga
       61 cctgcccgac gagctcggtg gtgagcccgt gttccactac tgggaggtgc aggagggcga
      121 ccactcccag cgcggggcga ccctcgaaga gacggcgcct gtcgtccacc tcgacctgca
      181 cgagccgctg ctcgaccgcg aaggcacgct gctccacccc ggcccgtggg ggatctactt
      241 ccgtcccagc gtgtcgggcg gagtcacggg cggagggctc cccaccccgc tctcaccgga
      301 ctgcgcgctc gacccgtacg gcccgtcgca ccccgaccac ggcgtcggcg acccggagca
      361 ggcggccggg ttcactgcgg gcctcgagag ggcactcggg cgcttcggcg aggacacgtg
      421 gcgcagcgag tgcttccggg cgccgatgtg cttcacggcc gactcccatc cgctcgtcga
      481 ctgggtgcgg ccgaacgcct acgccctcat cgactcgaac cacggcttca agctgctggc
      541 gctcggaagg ctcgcggccg ccgaactcgt cggtggccat gcggtcgagc tcgacccgtt
      601 ccggctcgag cgcttcgcga gagccgccgt gcatccggtg tccgccagcc cctacccctg
      661 gacctagcgc cgaacgagca gcccaccctt cgcctcggcg ccctccagga agcgcaccca
      721 gtcctcccac agcgccacgt cggtgagcgc gatcggccga gccgagatgc tttcgagcgc
      781 ctcggcgacc tcctgcggcc ggatcgtcag ctcctcctcg gcggattcga tgcgatcggc
      841 aagcgcgccg cagccctgca acgccatctc gtctgcgagc cgccgcgcgt cgtcggccga
      901 cagccacacg tgcccgtgga cgccgtcgtt ccaacgccac cagagccggt agctcacgac
      961 tccttcgcct tgcggcgcga cttctcgtag acggggtgct cgacgacccg tccggtgaca
     1021 tccgacccgt cgacacgagc gaccacgacg gtccccggca cgttcaagtc cttgcggatc
     1081 ttcgccaggc cgagggttcg gccgtcgagg cagggggcct ccactgcctg ggtgacgagc
     1141 ccgacctcca cgccgtcgac cgacagcggc gcaccgctgg ccgcatcccc gccgccgtcg
     1201 agctcgacgc tcgtgaggcg aagctcggcc gcgtcacggt cgcgcaagag gccctcccgc
     1261 ccgcgcacgt cggccttgtc gagcgcgacc gaccagccga gcccgcactc gtacggcgag
     1321 acgctcgggt cgtactcgac gccgccgatg atgaacccgc cctcgatccg gaagagatcg
     1381 agcgcgacca tcccgatcac cttcatcccc tgcggcgtca gggcctcgat gagagcatcc
     1441 cacagctcga gggcacgatc ccgctcgacc cacagctcgt aaccgagctc ggccgtgtag
     1501 ccgaggcgcg tcatgaaggc cggcacgccc gcgatctcga cgtcctcccg gaacgtgtag
     1561 taggggaagg cggcgttgga gaggtcgctc tcggtcagcc cctggagcat ggcgcggctc
     1621 tgtggtccct gcaggcagag atggggcgtc gcgtcggtca cctcgcgcac ctcgactccc
     1681 tgcggcgcct tgtcgcggaa gatctcgtag tcgcggtcgt tcgccccgca gaaacgaatg
     1741 ctgtcggccg agcgcagcat cacagtgcag tcgtcgacca tcttcccgtc ctcgtcgcag
     1801 agcgccccgt aggcgatctg gccgggtgcc agcgtagcga cgtcgcgcgc gacgatcgtg
     1861 ttcacgaacg cctgcgcgcc cggcccgtcg atgtcgactt tgcggagcat cgtgaagtcc
     1921 atcagcgcgg ccgcttcgcg gcaggcgcgg tactcctcct gcgtgtccga gacgacggag
     1981 ggcgccctgt acccgaagag atccatccag tcgtcggtca tctcccgcag gcgcggatag
     2041 aacgcggtct ccttgctcac ggcgatgctc tcctttgtcg cggtccggcg aacactaagc
     2101 tcccccggcc tcccccgcac ccagcggcgc cggcgtcgtc ccaagcccga gcgcctcgta
     2161 gacgagctcg gagagctcgc gcagctcgat ctctccctcg tgcccggacg tcttgatcgc
     2221 gtcctcgtac atcgtcacgt ccttcgggca cgcggtgacg aagtatttga tctctcggag
     2281 tgcgaccgct tcgtggatcc ggctctcgct cgggcggggc gcgtcgccct ccttcagctc
     2341 gtccatccag atgcgtccgc cgcctgcgcc gcagcagaac gagttgctgc ggttgcgcgg
     2401 catctcgacg agctcgcagc cgatggcggc gagcacgttc cgcggggcgt cgaagacgcc
     2461 gttgtagcgg ccgaggtgac aggggtcgtg gtacgtgacg cggacgtcga gcgtcttcgc
     2521 gggagcgatc gcgccggact cgagcagctc gaggaggagc tgcgagtggt ggacgacgtc
     2581 ccattctccg ccgagcgccg ggtactcgtt gcggatcgtg ttgaaggagt gcgggtcgga
     2641 ggtgaggatg cgctcgaact cgcagcccga gatcgcctcg atgttgttct cggcgagatc
     2701 caagaacagg acctcctccc ccacccggcg gacgtcgttg ccggcgttgc gctccgcctc
     2761 gtacaggatt ccgaaatcga cacccgcgtg gccgaggatg cgggcgatcg tttgcgagat
     2821 gccgctgttg cgcgggtcga gcgatgcgta gtcgccgacg aaccagagca gctcgaccgg
     2881 ctccttccgc gcgtccttga cctcgagctc cttggcccag cgcccacgct tccgcttcgg
     2941 ctcgccgaac gagttgcccg agtcgtagat ggtctccagc gtcgactgga gcgacgggtc
     3001 gagctccccc cggtcgacga gccgacggcg cagctggttg atgatcggca cgtgctcgat
     3061 gccgaccggg cagatctcga cgcacgccat gcactgcatg cacgaccaca gcgtctccgg
     3121 ccggatcgtc tggccgacga tctcggcctc gggctcgtag acgggcgtga ttcccatcgc
     3181 ggagcgcgcc ccgtaggtgc cctccgactg ctcgcggagg tcgagcacga ggtcgcgggg
     3241 cgagagcggg tagccggtgg ccgccgcggg gcacgcggcg tggcacttgc cgcacttcgt
     3301 gcaggcgtcg agctggagga ggtaggtggg cgccagatcg cgcacgacgc cgactccgac
     3361 ctcctcgggg ctcgggtcgt cggggagcgg gtcgagcgcc cttcccgatc gttcgttccg
     3421 cgtcgccacg ctcgccgggc cggtgagcat gtgcactgcc ttcgtgaagg ggatggcgga
     3481 gacgaaggcc agcgccgcga cgccgtgcac ccaccacaat ccgaagcggg ccgtgtcggc
     3541 accgtccccc tccaccccga tgccccgcag cgcctgcccg aagagccatc ccagcggcgc
     3601 ccacttctcg aacgacgggt cgtccaccgc gatccggagc gcctcgagca ggaagccggt
     3661 ggcgccgagg aagaacagcg agccgacgaa gatccagtcg cccgacacgt agcggcgccg
     3721 gtcgtagtcc ccgggcctac cgtcgacccg cgcgtagtcg agccgcgggg gcccgctcag
     3781 ccgtttcgcg gcgaagagga ccagcccgac gaggagcgcg aagccgaaga cgtcgaggaa
     3841 gagcgagtag ccgaggtaga agggcccctc ccagaacgtc cagccgaaga gcggctcggc
     3901 gacgtcgtcc tggaaggcga ggatggcggt cccgatgaag aggacgagga agccgtagaa
     3961 gacgagcagg tgcgccgtcc ccgcgacccc gtcgcggcgc cg
//
LOCUS       DBNGNK010000356         4846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1780927,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000356 DBNGNK010000000
VERSION     DBNGNK010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1780927"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="AC150V_10585"
     CDS             <1..1493
                     /locus_tag="AC150V_10585"
                     /inference="COORDINATES: protein motif:HMM:NF044955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2207 family protein"
                     /protein_id="HIR3763405.1"
                     /translation="RYTITGLAVAYDDVVDVNLQVWGGEWPVGLSVLEANQEAPGQIV
                     RGWGHPVWIRGDVTLSGSRARLRAVSVPPEQFVELRTLIPRNAFSSTAGMRVESGNGL
                     DRIVAEEASDAAEYQRDRDRIDDAVAHPWRTALYLALLAIGPAFFVISLVFLLFGRER
                     GTDYDREYEQEPPTDTEPALVPTLLRQGGGAGSYEFTATLFDLVRRGVYESKPVTTKR
                     TIWGGLRTEEVADLELSPGTDTKLRAWERAVADVVDGVLDGGSARLSLFRDKIEDERS
                     TMAPRFTRFKSDVATAVGKLRWFSSTGVALLGLALVVFLLLGGILLWQAVDGWRTVFP
                     RWSDVVLLAIGVCSLVNAALVFLALLLRRLWRRRTPAAQAEAERWQAFRRYLSDFPRL
                     HEAPPATLDLWERYLVYGIAFGIADRVLQGAQLHMPEEIAAASTVYWISTGPDLGSGA
                     TQMAINDLASGFGEALAPPNSGGGGGGGGFSGGGGGGGGGGGGGAW"
     gene            complement(1512..2183)
                     /locus_tag="AC150V_10590"
     CDS             complement(1512..2183)
                     /locus_tag="AC150V_10590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR3763406.1"
                     /translation="MITSRDNAKLKLVRRLQRRRQRESTGLFAAEGEDLAAAAEAAGI
                     EPVELLVAGENVEAELLGGVSTLPHPARVIGVYRQADLPRGGRAVTLALWRVTDPGNV
                     GTLLRTADAFGAAVALSPGCADPLGPKALRASAGAVFRVPLSGWGSLGGHRVALVAHG
                     GTPLAELALAPPVTLLLGGEREGLPENIADCDKATISLPGDAESLNVAAAGAIALYEV
                     TRARA"
     gene            complement(2220..2594)
                     /gene="rplT"
                     /locus_tag="AC150V_10595"
     CDS             complement(2220..2594)
                     /gene="rplT"
                     /locus_tag="AC150V_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR3763407.1"
                     /translation="MPRVKRSVAAKKKRRKVLAQAKGYRGLKKSHYRYAKEQVDRSLS
                     YAYRDRRNRKRTMRSLWIVRINAAARANGLSYNRFISGVQRAGIELDRKSLADIAVND
                     PQAFTTIAERAKAALEAESKAA"
     gene            complement(2597..2731)
                     /locus_tag="AC150V_10600"
     CDS             complement(2597..2731)
                     /locus_tag="AC150V_10600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL35"
                     /protein_id="HIR3763408.1"
                     /translation="MRRRHAMKSHNLEKKSSKRRRGFRKEYEVAPSDRKSVEKLLGRR
                     "
     gene            complement(2673..3359)
                     /gene="infC"
                     /locus_tag="AC150V_10605"
                     /pseudo
     CDS             complement(2673..3359)
                     /gene="infC"
                     /locus_tag="AC150V_10605"
                     /inference="COORDINATES: protein motif:HMM:NF017049.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            complement(3547..>4846)
                     /gene="thrS"
                     /locus_tag="AC150V_10610"
     CDS             complement(3547..>4846)
                     /gene="thrS"
                     /locus_tag="AC150V_10610"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR3763409.1"
                     /translation="ERNTQLTRIYGTAFFSQADLDAYLERLEQARARDHRRLGRQLDL
                     FHFDEHSPGSPLWHPKGMVLYNELEAVRRRENERRGYLEVKTPLIYDKALWVTSGHWD
                     KFREDMFLIPGEDEDHAYGVKPMNCPGHMLLFGSQLRSYRDLPIRYAEAAPLHRNELA
                     GALHGLTRVRYVTQDDSHIFCTEEQVPAEVDGVIDFVRYLYGLFGMEAHAELSTRPEN
                     RLGTDEQWDHAEGALEDALRRHGLEYVVSPGEGTFYGPKIDLHMTDVLGRSWQMGTIQ
                     YDMQMPARFGLTYMGPDNAEHMPIVIHRALYGSFERFIAILIEHFAGAFPFWLAPVQL
                     RVIPVGEDHREDASAVADVVRRAGFRVEVDRRDETVGKRIRDAEVEKVPYVVVYGDRE
                     SEDSLALRVRGGEQVTSSLAALVDRFRDEAAVAGGGAASG"
BASE COUNT          698 a         1680 c         1646 g          822 t
ORIGIN      
        1 tccggtacac gatcaccggg ctcgccgtcg cctacgacga cgtcgtcgac gtcaacctcc
       61 aggtctgggg cggcgagtgg ccggtcgggc tgagcgtcct cgaggcgaac caggaggccc
      121 cggggcagat cgtccgcgga tggggccatc ccgtgtggat tcgcggcgac gtgacgctga
      181 gtgggagccg agcgaggctc cgcgccgtca gcgttccgcc ggagcagttc gtcgagctcc
      241 gcacgttgat accccggaat gccttctcct cgaccgcggg aatgcgcgtc gaaagcggga
      301 acggcctcga caggatcgtc gccgaagagg cctccgacgc cgccgagtac cagcgcgatc
      361 gcgatcgcat cgacgacgca gtcgcccatc cgtggcgaac agctctatac ctcgcgctcc
      421 tcgcgattgg ccccgcgttc ttcgtgatca gcctcgtctt cctcctcttc gggcgggagc
      481 gcggcaccga ctacgaccgc gagtacgagc aggagccgcc gaccgacacc gagcccgcgc
      541 tcgtgcccac cctgctcagg cagggcggcg gcgcggggtc gtacgagttc accgcgacgc
      601 tgttcgacct cgtccggcga ggcgtctacg agtcgaagcc ggtcacgacg aagcgcacga
      661 tctggggcgg cctccgcacc gaggaggtcg ccgatctcga gctgtcgccg ggaacggaca
      721 cgaagctccg ggcgtgggag cgcgcggtcg ccgacgtcgt cgacggcgta ctcgacggcg
      781 gcagcgcacg gctctcgctc ttccgcgaca agatcgagga cgagcgctcg acgatggcgc
      841 cgcggttcac ccgtttcaag agcgacgtcg caaccgcggt gggcaaactc cgctggttca
      901 gctcgaccgg cgtcgcgctg ctcggcctcg cactcgtcgt cttcttgctc ctgggaggca
      961 ttctgctctg gcaggccgtc gacggatggc gcaccgtctt cccgcgctgg agcgacgtcg
     1021 tgctgctggc gatcggcgtc tgctcgttgg tcaacgcggc gctcgtgttc ctcgccctgc
     1081 tcctgcggcg gctgtggcgg cgtcgcactc cggcggcgca agcggaggcc gagcgatggc
     1141 aggccttccg ccgctatctg agcgacttcc cacggctcca cgaggctcca cccgcaaccc
     1201 tcgacctctg ggagcgctac ctcgtctacg gcatcgcctt cgggatcgca gaccgggtgc
     1261 tgcagggagc gcagctgcac atgccggaag agattgccgc cgcgagcacc gtctactgga
     1321 tctcgaccgg gcccgacctg ggctccggcg ccactcagat ggcgatcaac gacctcgcct
     1381 cgggcttcgg cgaggcgctc gcgccgccga actccggcgg cgggggcggc ggaggcggct
     1441 tctcgggcgg gggcggcgga ggaggcgggg gcggcggcgg gggcgcctgg tagccgccgg
     1501 cccgcgccgc atcaggctcg agctcgggtg acctcgtaga gcgcgatcgc gccggcggcg
     1561 gcgacgttca gcgactcggc gtcgcccggc agcgagatgg tcgccttgtc gcagtctgcg
     1621 atgttctcgg gcagcccctc gcgctcgcca ccgaggagga gcgtcacggg cggcgcgagc
     1681 gcaagctctg caagcggcgt gccgccgtga gcgaccagcg cgacccggtg accgcccagc
     1741 gacccccagc cggagagcgg gacgcgaaag actgcccccg ccgaggcgcg caaggctttc
     1801 ggcccgagcg gatctgcgca ccccggagag agcgcgaccg ccgcgccgaa cgcatcggcc
     1861 gtccggagca gcgtgccgac gttccccggg tcggtgacgc gccatagagc gagcgtgacc
     1921 gcgcggccgc cgcgcggcag gtcggcctgc cggtacacgc cgatgacgcg agccggatgg
     1981 ggaagcgtcg agacaccgcc gagcagctcc gcctcgacgt tctcgcccgc gacgagcaac
     2041 tcgaccggct cgatccccgc cgcctcggca gcggccgcaa gatcctcccc ctcggcggcg
     2101 aagagacccg ttgactcacg ctgacgacgc cgctggagcc tgcggacgag cttgagcttc
     2161 gcgttgtccc gcgacgtgat cacgcgaggc gccgcgtcgg catgcggacc tactcgcggt
     2221 caggcggcct ttgactccgc ctcgagcgcc gccttggccc gctccgcgat cgtggtgaag
     2281 gcctgcggat cgttgaccgc gatgtcggcg agcgacttgc ggtcgagctc gatgcccgca
     2341 cgctgcacgc ccgagatgaa gcggttgtac gagagcccgt tcgcccgcgc cgcggcgttg
     2401 atgcgcacga tccagagcga gcgcatcgtt cgcttgcggt tacgccggtc gcggtaggcg
     2461 tagctgaggg agcggtcgac ctgctctttc gcgtagcggt agtgggactt cttcagccct
     2521 cggtagccct tggcctgcgc cagaaccttg cggcgcttct tcttggcggc gacggatcgc
     2581 ttgacgcgag gcatcgctac ctccgcccca ggagcttctc gaccgactta cggtcgctcg
     2641 gcgcgacctc gtattccttg cgaaagcccc gcctacgctt cgaggatttc ttctcgaggt
     2701 tgtggctctt catcgcgtga cgacggcgca gcttcccggt tccggtcagc ttgaacctct
     2761 tctgtgcccc gctcgccctc ttctgcttcg gcatccgttt tcactcctgc attcttcgtc
     2821 ggtcccagca acatcaccat gtttcggccg tcctggacgg gagggttctc gacctggccg
     2881 atgtccttga cgtcctcggc gagccgcatc agcaagtcgc gaccgcgctc ggggtggagg
     2941 ttctcgcgcc cccggaacat gatcgtcgcc ttgaccttcg cacgctggtt gaggaagcgg
     3001 atcacgtgcc cccgcttcgt ctcgtagtcg tgcttgccga tcttcggccg gaacttgatc
     3061 tctttgacgt tgacctggga ttggtgtttg cgggcgagct tggccttctg ttcctgctcg
     3121 tagcggtact tgccgtagtc catgaccctt gccacgggcg ggtcggcgtt cggtgcgacc
     3181 tcgacgaggt cgaggttctg cgcgtacgcc tactcgcggg cctcatcggt cgacttgata
     3241 ccgatctgcg acccgtccga gtcgacgagc ctgacgcggg ggacgcggat cttctcgttg
     3301 atccgatcct gctggacgac gggccgggtc ctgtcgacag gacggcggcg cggtctcaaa
     3361 ggctgctcac caagccgatg ctgttcctct ctctcgaacg acgaaaccgc tgcgcaggca
     3421 gcggcactct cctcgtcgtc tcgactcggt tgaacctccg gggcgcgtcg gcctggaagg
     3481 tgaggggcgt gtccgctcct gcttcagcag agtcggcatc gtagcagcct tgcacgccgg
     3541 gctcgctcaa cccgacgcag caccgcctcc ggccaccgcc gcctcgtcgc ggaagcggtc
     3601 gacgagcgca gcgaggctcg acgtcacctg ctcgccgcct cgcacgcgga gcgcgagcga
     3661 atcctcggac tcgcggtcgc cgtagaccac cacgtacgga accttttcca cttcggcgtc
     3721 gcggattcgc ttcccgacgg tctcgtcgcg ccggtcgacc tcgacccgga agcctgcgcg
     3781 ccgcacgacg tccgcgacgg cggacgcatc ctcgcggtga tcctcgccga ccggtatgac
     3841 gcgaagctgc accggcgcca gccagaaggg gaacgcaccg gcgaagtgct cgatcaggat
     3901 ggcgatgaag cgctcgaacg aaccgtagag cgcacggtgg atgacgatcg gcatgtgctc
     3961 ggcgttgtcc ggccccatgt acgtgagacc gaaccgcgcc ggcatctgca tgtcgtactg
     4021 gatcgttccc atctgccagg agcgtccgag cacgtccgtc atgtggagat cgatcttggg
     4081 cccgtagaac gttccctcgc ccgggctgac gacgtactcg agaccgtgac gacgcagcgc
     4141 gtcctcgagc gctccctccg cgtgatccca ctgctcgtcg gtgccgaggc gattctccgg
     4201 gcgtgtcgag agctcggcat gagcctccat gccgaagagt ccgtagaggt agcgaacgaa
     4261 gtcgatgacg ccgtcgacct ccgccggaac ctgctcctcg gtgcagaaga tgtgcgagtc
     4321 gtcctgcgtg acgtaacgga cgcgtgtcag cccgtggagg gcgccggcga gctcgttccg
     4381 gtgcagaggc gctgcctccg cgtagcggat cggcaggtcg cggtagctac ggagctggct
     4441 cccgaacaac agcatgtgcc cgggacagtt catcggcttg acgccgtagg cgtgatcctc
     4501 gtcctcgccg gggatgagga acatgtcctc ccggaacttg tcccagtggc ccgaggtgac
     4561 ccagagcgcc ttgtcgtaga tgagcggcgt cttgacctcg aggtagccgc gccgctcgtt
     4621 ctcgcgccgc cgcacggcct cgagctcgtt gtagagcacc attcccttcg ggtgccagag
     4681 cggagacccc ggcgagtgct cgtcgaagtg gaagaggtcg agctgtcgtc ccaaccgccg
     4741 gtggtctcgc gcgcgggcct gctctagccg ctccagatag gcatcgaggt cggcctgaga
     4801 gaagaaggcc gtcccgtaga tgcgagtcag ttgcgtgttg cgctcg
//
LOCUS       DBNGNK010000357         4673 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1781314,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000357 DBNGNK010000000
VERSION     DBNGNK010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1781314"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1371
                     /locus_tag="AC150V_10615"
     CDS             67..1371
                     /locus_tag="AC150V_10615"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR3763410.1"
                     /translation="MIDVPVALLGYGTVGAAVHRLLEESADEIERTTGHRLRVVRALV
                     RDTDKQRDQSPDGSVLTTDFAAIRDDPDIVVVAEVMGGLEPACPYVLELLESGRPVVT
                     ANKQLVAQRWPDLFAAAAEGGVQLRFEAAVAAAIPVIKVLRESLVVTNVHRVLGIVNG
                     TTNYILGEMEGGRSYAEALDEAQRLGYAEADPAEDVGGQDAGAKMAILATVAFGSRVP
                     LEWVDVKGIEHVTQEQVAAARALDLRIKLVGRATLIGDRVDVRVGPALVDTHHPLAAV
                     GGAFNAVMLQGDAIREITLEGPGAGGVETASAVVADLVSIVGTTGTGFLQSDPVRRTL
                     ERLPAGELPSPHYLRLEVEDRSGVLAVLSQKFADENVSVARLVQQPAGEGAAVLHVVT
                     HEAATGKVERALAAIGALPVSRGDATAMPVVSDRGVEELGWA"
     gene            1368..2333
                     /gene="thrC"
                     /locus_tag="AC150V_10620"
     CDS             1368..2333
                     /gene="thrC"
                     /locus_tag="AC150V_10620"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR3763411.1"
                     /translation="MTGSLIDRYRGSLPVTRRTPVVTLGEGSTPLLRADHLSARLGLE
                     LFLKWEAANPTGSYKDRGMTVAVSKAVEEGAEAVICASTGNTAASAAAYAARAGIPAL
                     VLVPEGAVSGAKVAQTRMLGAKVLEARGSFTEALAAVRALAERGRHALVNSLNPYRIE
                     GQKTAVFEIAEDLGRIPDAFVIPYGGGGNTSAYAKGLEELGSDATIVSAESSQRPTTL
                     ASAIRISDPVHAERVAGSGAQVVSIDDAEIIDAWRELAAVEGLFCEPSSAAGLAAVKK
                     GAVTGNVIVCTLTGHGLKDIAAAEKYAPRAEKVDPDPDAIARAAR"
     gene            2330..3145
                     /locus_tag="AC150V_10625"
     CDS             2330..3145
                     /locus_tag="AC150V_10625"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR3763412.1"
                     /translation="MIIRAPATSANVGPGFDCAAVALGLWNELELTDGEGVVVEGQGA
                     GELPETADNLAVRAYGLLADHGAKRFVFKNRIPLERGLGSSAAAIALGLVAAAPDATA
                     ERLLESGLELESHADNLAAALVGGVTLTWNGRIARIASTLPLSPVAVVPQGRTGTEQA
                     RAALPELVSHSDAAVTAARAALLGAGAVAADAELFAAALDDRLHEPYRPSKILDAVRS
                     DPPAGSRGATLSGSGPTVIVWADDVSRCEADLRERFTEHDVLPLTIPDHGALR"
     gene            3142..3858
                     /locus_tag="AC150V_10630"
     CDS             3142..3858
                     /locus_tag="AC150V_10630"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR3763413.1"
                     /translation="MIVLVDCRPSDAYRAGHVPGAAHLDPERDLTGEIGDVGRGRHPL
                     PDPESAAAAVGRAGVGAKSFVLAYDDGSGWAARCWWLLRHAGHDAAGTFDIRAYAGPL
                     DTGNDGAAGDFARESRFVEHMRNDDVIEAEEILDRLGDPSLLLLDARAEERWAGESEP
                     LDPVAGRIPGARNAFFQHPLPDGILDAAEIAVYCGSGVSACVTLQQLVLAGRPDARLY
                     PGSFSEWCRREGYPIEKGRL"
     gene            3909..>4673
                     /locus_tag="AC150V_10635"
     CDS             3909..>4673
                     /locus_tag="AC150V_10635"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR3763414.1"
                     /translation="MTEGIDRAGARSMLHAVGFTDEDLSKPIIGVATTWIETMPCNLN
                     QRDLAVDVKRGIREAGGTPMEFNTIAVSDGVSMGTPGMRASLVSREVIADSIELVARG
                     HMFDGLVCLVACDKTNPGAVMALARLDLPSVVLYSGSIAPGVYRGHEVSLGDVYEGIG
                     AYAAGKTSAEELHELALQACPGAGACGGQFTANTMSTMLEFLGLSPAGLNGIPALSKR
                     KKEAAEECGRLVMDLVRRDVRPSHIVSLDAIENAAAS"
BASE COUNT          701 a         1631 c         1670 g          671 t
ORIGIN      
        1 cgagaccgtg gggaaggagc tccgcgagct catgcaccgc gccggcgggg atccgacggt
       61 gcagccgtga tcgacgtccc ggtcgccctt ctgggctacg ggacggtcgg cgcggcggta
      121 caccgcctgc tggaggagag cgccgacgag atcgagcgca cgacgggcca ccggctgcgg
      181 gtcgtgcggg cgctcgtccg cgacacggac aagcagcgcg accagtcccc cgacgggagc
      241 gtgctgacaa ccgacttcgc cgcaatccgt gacgatcccg acatcgtcgt cgtcgccgag
      301 gtgatgggcg ggcttgagcc cgcctgtcca tacgtcctcg agctgctcga gagtggacgg
      361 ccggtcgtca cggcgaacaa gcagctcgtc gcgcagcgct ggcccgacct gttcgcggcg
      421 gccgccgagg gcggcgttca gctccgcttc gaagcggccg tcgcggcggc cataccggtc
      481 atcaaggtgc tccgggaatc gctcgtggtg acgaacgtcc accgcgtgct cggcattgtc
      541 aacggcacga cgaactacat cctcggcgag atggagggcg gtcgcagcta cgcggaggcg
      601 ctcgacgagg cgcagcgtct cggctacgcg gaggccgacc cggcggagga cgtcggcggc
      661 caggatgccg gggcgaagat ggcgatcctg gccacggtag cgttcggctc gcgcgtgccg
      721 ctcgagtggg tcgacgtgaa agggatcgag cacgtcacgc aggagcaggt cgcggccgct
      781 cgggcgctcg acctccgcat caagctcgtc ggacgggcca cgctgatcgg cgatcgggtc
      841 gacgtgcggg tcgggcccgc gctcgtcgac acccaccacc cgctcgccgc cgtcgggggc
      901 gctttcaacg ccgtcatgct ccagggcgac gcgatccggg agatcacgct cgagggcccc
      961 ggagccggcg gggtcgagac cgcctccgcc gtcgtcgccg acctcgtgtc gatcgtgggg
     1021 acgacgggaa ccggcttcct ccagagcgac cccgtccgac gaacactcga gcggcttccg
     1081 gccggcgagc tgccgtcgcc gcactacctg cggctcgagg tcgaggaccg ctccggcgtg
     1141 ctggccgtgc tctcgcagaa gttcgccgac gagaatgtct cggtcgcccg tctcgtgcag
     1201 caacccgccg gcgagggagc tgcagtgctg cacgtcgtca cgcacgaggc cgcgacggga
     1261 aaggtggaga gggcgctcgc ggcaatcggg gcgctccccg tctcgcgcgg cgacgcgacc
     1321 gcgatgcccg ttgtctctga ccgaggagtc gaggagctcg gctgggcgtg acaggctcgt
     1381 tgatcgaccg ttatcgtggc agcctgcccg tgacccggcg gacgccggtg gtcacgctcg
     1441 gcgaagggtc gacgccgctg ctgcgggcag atcacctgtc cgcacggctg ggtctggagc
     1501 tcttcctcaa gtgggaggca gcgaacccga cgggcagcta caaggaccgc ggcatgaccg
     1561 tcgccgtctc caaggccgtc gaagagggtg cggaagcggt gatctgcgcg tcgacgggga
     1621 acaccgcggc gtcggcagcc gcctatgcgg cacgggccgg catcccggcc ctggtgctcg
     1681 tcccggaggg cgccgtcagc ggggccaagg tcgctcagac acgcatgctc ggcgccaagg
     1741 tgctcgaagc gcgcgggagc ttcaccgagg cactggccgc cgtccgtgct ctggcagagc
     1801 gcggtcggca cgcgctggtc aactcgctga acccgtaccg catcgagggg cagaagacgg
     1861 cggtcttcga gatcgccgaa gacctcggcc ggatccccga cgcgttcgtc attccgtacg
     1921 gcggtggggg caacacctcc gcctacgcca agggcctcga ggagctcggg agcgacgcga
     1981 cgatcgtctc cgccgagtct tcgcaacggc cgacgacgct cgcatcggcg attcgcatct
     2041 ccgatcccgt tcatgcggag cgcgtggcag gcagcggagc gcaggtcgtg agcatcgacg
     2101 acgccgagat catcgatgcg tggcgcgagc tcgccgcagt cgaagggctc ttctgcgagc
     2161 cgtcgtcggc ggcggggctg gccgcggtga agaagggcgc ggtgaccggg aacgtcatcg
     2221 tatgcacgct gacgggccac ggcctcaagg acatcgcggc cgccgagaag tacgcgcctc
     2281 gggccgagaa ggtcgatccc gaccctgacg ccatcgcgcg cgcggcccga tgatcattcg
     2341 cgctcccgca acctcggcga acgtcggacc cgggttcgat tgcgcggccg tcgccctcgg
     2401 gctctggaac gagctcgagc tgaccgacgg agagggcgtg gtcgtcgagg gtcagggcgc
     2461 cggtgagctg cccgagaccg cggacaacct cgccgtccgg gcctacggac tgctcgccga
     2521 ccacggcgcc aaacggtttg tgttcaagaa caggattccg ctcgagcgcg gcttgggctc
     2581 gtcggcggcc gcgattgcac tcggcctcgt cgcggccgcg cccgacgcca cggccgagcg
     2641 cctgctcgag tcgggcctcg agctcgagag tcatgccgac aatctcgctg ctgcgctcgt
     2701 cggcggggtc acgctgacct ggaacgggcg gatcgcccgc atcgcctcga cgctgccgct
     2761 gagcccggtt gcggtggtac cacagggacg cacgggcacc gagcaggccc gcgcggcgct
     2821 gcccgagctc gtttcgcaca gtgacgccgc ggtgacggcc gcgcgggcgg cactcctcgg
     2881 cgcgggtgcg gtcgccgccg acgccgagct gttcgcggct gcgctcgatg accggctgca
     2941 tgagccctac cggccgtcga agatcctcga cgcggtccgc tccgacccgc cggcgggctc
     3001 gcgcggagcg accctgtccg gctccggccc gaccgtgatc gtgtgggccg acgacgttag
     3061 ccgctgcgag gcagacctgc gcgagcggtt caccgagcac gacgtgctcc cgctgacgat
     3121 ccccgatcac ggcgcactca gatgatcgtc ctggtcgact gtcgaccgtc cgatgcgtac
     3181 cgcgccggcc acgtccccgg agcggctcac ctcgatcccg agcgggatct gaccggcgag
     3241 atcggtgacg tcggccgtgg gcgccacccg ctgcccgacc ccgagtcggc tgcagccgcc
     3301 gtcggccggg cgggagtcgg ggccaagtcc ttcgtcttgg cctacgacga cggcagcggc
     3361 tgggccgcac gctgctggtg gctgctccgc cacgccggtc acgacgcggc gggaacgttc
     3421 gacattcgtg cgtacgctgg cccgctcgat accggcaacg acggcgcggc cggagacttc
     3481 gcgcgagaat cacggttcgt ggagcacatg cgcaacgacg acgtgatcga agccgaggag
     3541 atcctcgacc gcctgggcga cccgtcgctg ctcctgctcg acgcccgagc ggaggagcgc
     3601 tgggccgggg aatcggagcc gctcgatccg gtcgcaggcc ggattcccgg cgcgcgtaac
     3661 gccttcttcc agcacccgct cccggacggc attctcgacg ccgccgagat cgccgtctac
     3721 tgcggctcgg gcgtgagcgc ctgcgtgacg ctccagcaac tcgtcttggc gggtcgcccg
     3781 gatgcacgcc tctaccccgg ctccttcagc gaatggtgcc gccgcgaggg atatccaatc
     3841 gagaaaggac ggctgtgagc actccctatg acaagcccgt tgatcccgcg aagcgacaca
     3901 gcgcgaccat gacggagggc atcgaccgcg ccggcgcccg gtcgatgctc catgcggtgg
     3961 gtttcaccga cgaggatctc tcgaagccga tcatcggcgt ggcgacgacc tggatcgaga
     4021 cgatgccgtg caatctcaac cagcgcgacc tcgcggtcga cgtcaagcgc gggatccgcg
     4081 aggccggtgg aactccgatg gagttcaaca cgatcgcagt ctcggacggc gtctcgatgg
     4141 ggactccggg catgcgggca tcgctcgtgt cgcgcgaggt gatcgccgac tcgatcgagc
     4201 tcgtcgcccg tggccacatg ttcgacggac tcgtctgcct cgtcgcctgc gacaagacca
     4261 acccgggcgc cgtgatggct ctggcgcgcc tggacctccc gagcgtcgtc ctctacagcg
     4321 gctcgatcgc gcccggcgtc tatcgcggac acgaagtgtc cctcggggac gtctacgagg
     4381 ggatcggcgc ctacgcggca ggcaagacct cggccgaaga gctgcacgag ctggcgctac
     4441 aagcgtgccc gggcgccggc gcctgcggag gccagttcac cgcgaacacg atgtccacga
     4501 tgctcgagtt cctcgggctc agcccggccg gcctgaacgg aattccggcg ctctccaagc
     4561 ggaagaagga ggcggcggag gagtgcgggc gcctcgtcat ggatctcgtt cgccgtgatg
     4621 ttcgcccctc gcacatcgtc tcgctcgacg cgatcgagaa cgcagctgcc tcc
//
LOCUS       DBNGNK010000358         5720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1781403,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000358 DBNGNK010000000
VERSION     DBNGNK010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1781403"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="AC150V_10640"
     CDS             <1..441
                     /locus_tag="AC150V_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013988541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HIR3763415.1"
                     /translation="DFTTRFIELAGEVNQNMPYHCRSLVSQALNHGAGKSLKGSRVLI
                     LGVAYKADIADVRESPALKLIELLGNAGAEVAYHDPHVAELDEHGLRLTSAALEPADY
                     DAVVIVTAHSSIDYERLVDEAALVVDFRNATGRNDITSDKVWRL"
     gene            438..1415
                     /locus_tag="AC150V_10645"
     CDS             438..1415
                     /locus_tag="AC150V_10645"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3763416.1"
                     /translation="MSGVTVGIAGLGYWGPNLARNLEELAELAWLCDLDHDATARYSS
                     RHPAARTTPDFDEMLADDSLEAVVVSTPVPTHFELARRALHAGKHVLVEKPPAMRGSE
                     MDELVRLAVERDLVLMPGHLLLYHPGVRKLKELIEAGELGDVLCVYTNRQNLGIIRAD
                     ENALWSLGVHDLSVILWLVGEEPIEVVAHGRDFLQPGVEDVVFCYLRFPSGRVAHMHL
                     SWLDPHKMRKMTVVGTEKMVVFDDMELERKVTVYDKAPWQRAETYGEWQTRTGDIHIP
                     KVDTAEPLRLECQAFVELIRGDGDRRRVAEDGAMVVRALDRLTESLRAG"
     gene            1825..2052
                     /locus_tag="AC150V_10650"
     CDS             1825..2052
                     /locus_tag="AC150V_10650"
                     /inference="COORDINATES: protein motif:HMM:NF012360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HIR3763417.1"
                     /translation="MGRTDERFGTVAGPTIRRAARIGGGAVLCPGVEIGEEAFVGAGA
                     VVTKDVPPRVVVVGSPARILRDVPEDELLDG"
     gene            complement(2063..3703)
                     /locus_tag="AC150V_10655"
     CDS             complement(2063..3703)
                     /locus_tag="AC150V_10655"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR3763418.1"
                     /translation="MSKRLLILPVLVAALLASASADAARFAVGLSPDADASSVVEALE
                     RQGGRVENLSPLPALVVELPAGVAPAQLAGVRYVERLQERRLALNVNDPLAPRQWYLG
                     ANRAFDSWEVLPPLAAVRVAVIDSGVDLGHPELAKRVVAARSFVGGQPRDQQGHGTFV
                     AGIIAAETNNQMGIAGMAPGTELLVAKVVTADQTIPVEAEAKAIRWAVVNGARVINMS
                     LGGLRDPTDPARDTYSALEADAIAYATSQNVAIVAAVGNSDEAPTRPWRYASYPAALP
                     HVLGVSALSRAGESPEFSNRDAIFNDISAPGQEIVSTLPRSLTASARPACPEQGYSIC
                     GSDEYRSAEGTSFAAPQVSAAAAMLIATRPDLTSDQVTTVLSRSAVDVSAATGCQQCP
                     LGRDSLTGWGRLDVTGAVAALSGPLPTVDSLEPNDDAGEQAQPLWGAKRRIVATLDYW
                     DDQSDVYRIQLRRGERMFASLVGPKGTDTSLALWRPGTFAIDDLARQSFRLRIASRPG
                     PDEYLAHRSNATGFYYLQVKMTAEGSGAYRLTIVKRRR"
     gene            complement(3700..5082)
                     /locus_tag="AC150V_10660"
     CDS             complement(3700..5082)
                     /locus_tag="AC150V_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR3763419.1"
                     /translation="MTAARLSLAAAVLVAASAFVLGAGARPSAAPQGAVLFVLSGGGW
                     GHGVGMSQWGAFGQAKTGRKYGQILKHYYRGTTLAKVPGRKVRVLLADGARSLRISSA
                     TPFRVRAADGKVYRLAAGEITIGAALRLSVKGKPKKLPGPLRFRPGTGVPLALGGQAY
                     RGELVVAVEGKRLRAVNALSLQPYLMGVVAEEMPFDWPLEALKAQAVAARSYALARLV
                     KGKPFDLYADWRSQVYGGVGAEKPSTTRAVSETSGQVLLHRGKVATTFFHSTSGGRTA
                     AAADVYGPSFRPPYLRSVADPWDRDSPHHRWEPRALTPARLGKTLGLSSPVVDVETDK
                     ARSGHVTLVRFHTAAGGVHERSGNEVRDALALRSTWFRVGVLRFLQPAGKVKAGKPVR
                     LSGVARDVGSVELQRQLDGTWSRLRRLNPAADGTFRLVVRPGRTVRYRLATGEFAGAP
                     VTVRVAAAPR"
     gene            complement(5161..>5720)
                     /locus_tag="AC150V_10665"
     CDS             complement(5161..>5720)
                     /locus_tag="AC150V_10665"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR3763420.1"
                     /translation="KAGGDLQVLAYVRTYGVNASITRGATTYGPRQYPEKLVPLFVTN
                     ALDGHPLPLYGDGRQVREWLHADDHCAAIELVLREGAPGEAYNVGGEDFENREVTNRI
                     LELTGADDELVRHVEDRAGHDRRYALDDAKLRSLGWAPEHSFGELGLPQTVAWYRENR
                     DWWEPIKSGEYRAYYEQQYAARLRD"
BASE COUNT          894 a         2001 c         1970 g          855 t
ORIGIN      
        1 gacttcacga cccgcttcat cgagctcgcg ggtgaggtga accagaacat gccgtaccat
       61 tgccgctcgc tcgtctcgca ggcgctgaac cacggcgccg gcaagtcgct gaaagggtcg
      121 cgagtattga ttctcggtgt cgcctacaag gccgacatcg ccgacgtgcg ggagtcgccg
      181 gcgctcaagt tgatcgagct gctcggcaac gcgggggccg aagtcgccta tcacgacccg
      241 cacgtcgcgg aactggacga gcatggcctc cggctcacgt ccgccgcgct cgagcccgcc
      301 gactacgacg cggtggtgat cgtgaccgcg cactcgtcga tcgactacga acggctggtc
      361 gacgaggccg ctctcgtcgt cgacttccgc aacgccaccg gtcgcaacga catcacgagc
      421 gacaaggtct ggaggctttg agcggcgtca cggtcggcat cgccggcctc ggctactggg
      481 ggccgaacct cgcgcgaaat ctggaggagc tcgcggagct ggcgtggctc tgcgatctgg
      541 atcacgacgc cacggcgcgc tacagctccc gccaccccgc cgctcggacg acgcccgact
      601 tcgacgagat gctcgccgac gactcgctcg aagcggtcgt cgtgtcgacc ccggtaccga
      661 cccatttcga gctcgcccgc cgggcgctcc acgccggcaa gcacgttctc gtcgagaaac
      721 cgccggcgat gcgagggtcg gagatggacg agctggtgcg attggcggtc gagcgcgacc
      781 tcgttctcat gcccgggcac ctcctcctct accacccggg cgtgcgcaag ctgaaggagc
      841 tgatcgaggc gggagagctc ggcgacgtcc tctgcgtcta cacgaatcgg cagaacctcg
      901 ggatcatccg ggccgacgag aacgcgctct ggtcgcttgg cgtccacgac ctctcggtga
      961 tcctctggct cgtcggcgag gagccgatcg aggtcgtcgc gcacggccgc gacttcctgc
     1021 aaccgggcgt cgaggatgtc gtgttctgct atctgcgctt cccgtccggc agggttgcgc
     1081 acatgcacct ctcgtggctc gacccgcaca agatgcgaaa gatgaccgtc gtgggcaccg
     1141 agaagatggt cgtcttcgac gacatggagc tcgagcgaaa ggtgaccgtc tacgacaagg
     1201 ctccgtggca gcgtgccgag acgtacgggg agtggcagac acgcacgggc gacatccaca
     1261 ttcccaaggt cgacactgcg gagccgttgc ggctcgagtg ccaggccttc gtcgagctca
     1321 tccggggtga cggcgatcgc cgccgcgtgg ccgaggacgg cgcgatggtc gtccgggcgc
     1381 tcgaccgact gaccgaatcg ctccgtgccg gctgacgtcc acccgaccgc caccgtgcat
     1441 gcgggcaccg tgctcggcga gggtgtgaag gtactcgagg gcgcggtggt ggggaagcag
     1501 ccgaccctct cgccacgctc cacagccaag cgcgagccgc tcggcccgac cgttatcggc
     1561 gacggtagcg tggtctcgac aggggcgatc gtgttcgccg gctccacgat cggcgccaga
     1621 gtcattctcg gtgaccagtc gtgcgtgcgg gagcgggttt cgatcggaga cgacgtcgtg
     1681 gtcggccgag gatcactgat cgagaacgac acaacgatcg gcgcgttgac caagatccag
     1741 gccgatgcgt acatcaccgc ctactccacc ctggaggagc acgtcttcat cgcgccatgc
     1801 gtcgtcacga cgaacgacaa cctgatgggg cgcaccgatg agcgcttcgg caccgtcgcg
     1861 ggcccgacga tccgccgagc ggcacgcatc ggcgggggcg ccgtgttgtg ccccggcgtg
     1921 gagatcggcg aggaggcatt cgtgggtgcg ggcgctgtcg tgacgaagga cgtcccgcct
     1981 cgtgtcgtcg tcgtcggaag cccggctcga atcctgcgcg acgtccccga ggacgagctg
     2041 ctcgacggct agtgccggga cgctaccggc gccgcttgac gatcgtcagc cggtacgctc
     2101 cggagccctc ggccgtcatc ttgacctgaa ggtagtagaa gcccgttgcg ttcgaccggt
     2161 gcgcgaggta ctcgtcgggc cccggccgcg aagcgatgcg cagccggaac gactgccgcg
     2221 cgagatcgtc gatcgcgaag gtgccggggc gccacagggc cagtgaggtg tccgtcccct
     2281 tcggcccgac gagcgatgcg aacattcgct cgccgcgcct gagctgaatg cggtagacat
     2341 cgctctggtc gtcccagtag tcgagcgtcg ctacgatacg ccgcttcgcc ccccagagcg
     2401 gctgcgcttg ctctccggcg tcgtcgttgg gctcgagcga gtctacggtc ggcagcgggc
     2461 ccgacagggc ggcgacggct ccggtgacat ccaggcggcc ccagcccgtc agcgagtccc
     2521 gcccgagcgg gcattgctgg cagccggtgg cggccgacac gtcgaccgcg gaccgggaca
     2581 gcaccgtcgt gacctgatct gaggtcaagt ctgggcgtgt ggcgatgagc atcgcggcgg
     2641 cggcactgac ctggggggcg gcgaaggaag tgccctccgc agaccggtac tcgtcactgc
     2701 cgcagatcga atacccctgc tccggacagg ccggccgggc cgacgcggtc agcgagcgtg
     2761 gcagcgtcga gacgatctcc tggccgggcg ccgagatgtc gttgaagatc gcatcgcggt
     2821 tggagaactc gggcgactct cccgcccgcg agagcgcgct gactcccagg acgtgcggga
     2881 gcgccgccgg gtagctcgcg tatcgccacg ggcgcgtcgg cgcctcgtcg ctgttcccga
     2941 ccgccgcgac gatggcgacg ttctgcgacg tcgcgtacgc gatcgcgtcg gcctcgagcg
     3001 ccgagtacgt gtctcgcgcc ggatccgtgg ggtcgcgcag cccgccgagg ctcatgttga
     3061 tcacccgagc gccgttgacg accgcccagc ggatcgcctt cgcctcggcc tcgaccggaa
     3121 tcgtctgatc cgcagtcacg actttcgcga caaggagctc cgtgcccggc gccattccgg
     3181 caatacccat ctggttgttc gtctcggcgg cgatgatccc ggccacgaac gtgccgtggc
     3241 cttgctgatc gcgcggctga cccccgacga agctccgcgc agcgacgacc cgcttggcga
     3301 gctcgggatg tcccaggtcg acgcccgaat cgatgacggc cacccgcact gcggcgagtg
     3361 gcggcaacac ttcccacgag tcgaacgcgc ggttcgcccc tagataccac tgccgggggg
     3421 ccagcgggtc gttcacgttc agcgcaagac gacgctcctg caagcgctcg acataacgaa
     3481 cgcccgcgag ctgcgccggc gcaacccccg caggcagctc gacgacgagt gccgggagcg
     3541 gagacaggtt ctcgacgcgc cctccctgcc gctctagcgc ctcgacgacg ctcgacgcgt
     3601 cggcgtcggg cgaaagaccc accgcgaacc gcgccgcgtc ggcagaggcg gacgcgagga
     3661 gggccgcgac gaggacgggc aggatcagaa gtcgtttgct catctcggag ccgcagccac
     3721 ccgcaccgtg accggcgccc ccgcgaactc cccggtcgcg aggcgatacc gcacggtcct
     3781 gccgggacgg acgacgagcc gaaacgtccc gtctgccgcg gggttgaggc gtcgcagccg
     3841 ggaccaggtt ccgtcgagct gtcgctgcag ctcgacggac ccgacgtcgc gggcgacgcc
     3901 ggacaggcgg accggctttc ccgccttcac tttcccggcg ggctgcagga accggaggac
     3961 gccgacacgg aaccaggtcg aacggagcgc gagcgcgtcc cggacctcgt tgccgcttcg
     4021 ctcgtgaacg ccgcccgccg ccgtgtggaa gcgaacgagc gtcacgtggc ctgaccgtgc
     4081 cttgtcggtc tcgacgtcca cgaccggtga cgaaagaccg agcgtcttgc ccagccgcgc
     4141 gggcgtcagc gcgcgcggct cccagcgatg atgcggggaa tcgcggtccc agggatccgc
     4201 cacggaccgg aggtacggcg gccggaacga cggcccgtag acgtctgccg cggcggcggt
     4261 gcggccaccg gaagtcgagt ggaagaacgt cgtcgccacc ttgccccggt gcagcaggac
     4321 ctggccggat gtctcactga cggccctggt ggtgctcggc ttctcggcgc ctacgccgcc
     4381 gtagacctgg ctgcgccagt cggcgtagag atcgaacggc tttcccttca ccagcctggc
     4441 cagggcgtag gagcgggcgg cgacggcctg cgccttcagt gcctcgagcg gccagtcgaa
     4501 aggcatctcc tccgctacga ctcccatcag atacggctgc aggctgaggg cgttgaccgc
     4561 ccgcagccgc ttgccctcga cggcgacgac gagctcaccg cgataggcct gcccgccgag
     4621 cgcgagcggc acgcccgtcc cgggcctgaa tcgaagcggg ccggggagct tcttcggctt
     4681 gcccttgacg gacagcctca gcgcggcgcc gatcgtgatc tcgccggccg ccaggcggta
     4741 caccttgccg tctgcggccc gaacacggaa gggcgtagcg gacgagatcc gcaacgagcg
     4801 ggctccgtcc gcgagcagca cgcgcacctt cctacccggc accttcgcga gcgtcgttcc
     4861 ccggtagtag tgcttcagga tctggccgta cttccgcccg gtcttcgcct gaccgaaggc
     4921 gccccactgg ctcatcccga cgccgtggcc ccagccgcct ccgctcagca cgaagagcac
     4981 ggctccctgc ggcgcggcgg atggccgggc accggcgccg aggacgaagg ccgaggcggc
     5041 cacgagcacg gcggcagcaa gcgagaggcg ggcggcagtc acggcgacag ctcaggctag
     5101 ctgaggcctg taagcattcc tggcgaccgg cgcggcggtc gccgcacggc gcgcggagcc
     5161 tcaatcacgc agtcgcgccg catactgctg ctcgtagtac gcgcggtact cacccgactt
     5221 gatcggctcc caccagtcgc ggttctcccg gtaccacgcc accgtttgcg gcaggccgag
     5281 ctctccgaag gagtgctcgg gcgcccaccc gagagagcgg agctttgcgt cgtcgagggc
     5341 atagcgacgg tcgtgacctg ctcgatcctc gacatgccgg accagctcgt cgtcggcacc
     5401 ggtcagctcg aggatccggt tggtgacttc cctgttctcg aagtcctcgc cgcccacgtt
     5461 gtacgcctct ccgggcgcgc cttcgcgcag gacgagctcg atcgccgcgc agtggtcgtc
     5521 cgcgtgcagc cactcgcgca cctgacggcc gtcaccgtag agcggaagcg ggtgtccgtc
     5581 gagagcgttg gtgacgaaca ggggcacgag cttctccgga tactgccgcg gcccgtacgt
     5641 ggtcgcaccg cgggtgatcg acgcgttgac gccgtacgtg cgcacgtagg caagcacctg
     5701 caggtcgccg ccggccttcg
//
LOCUS       DBNGNK010000359         2834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1639322,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000359 DBNGNK010000000
VERSION     DBNGNK010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1639322"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC150V_10670"
     CDS             complement(<1..246)
                     /locus_tag="AC150V_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011739165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR3763421.1"
                     /translation="MSEAEVVPLPEPRPEERPVLVVDLGGQYSQLIARRVREARVYSE
                     LVGHGTSPEAIRARRPLAVILSGGPASVYAASAPRVDP"
     gene            complement(243..1730)
                     /gene="guaB"
                     /locus_tag="AC150V_10675"
     CDS             complement(243..1730)
                     /gene="guaB"
                     /locus_tag="AC150V_10675"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013276851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR3763422.1"
                     /translation="MELDFDREAKFGQEGLTFDDVLLVPAESAVLPNDVSTVTRLTRT
                     IAIEIPVVSAAMDTVTEARLAIALAREGGIGIVHRNLSIGDQVAEIDKVKRSEAGMIV
                     EPVTLPPEALVADALALMARYRVSGVPITDRAGVLVGILTNRDLRFGADPTQPVSAYM
                     TSRDLVTAPVGTTLAEAEAILGRHKVEKLPVVDADGLLKGLITVKDIQKKVKYPLASK
                     DERGRLRVGAAVGVGNDAIDRVAALAEAEVDVIVVDTAHGHSKGVLDVVRRIKDGWET
                     QVIAGNIATAAAAEAICDAGADGVKIGVGPGSICTTRVIAGVGMPQVTAIFDAAEVAQ
                     RYGVPIVADGGLTSSGDVAKAVAAGADTVMLGSMLAGTDESPGEVLLVQGERFKEYRG
                     MGSLGAMKARSFSKDRYFQGDVEDVDKLVPEGIEGRVPYKGPLAPIVYQLVGGLRQAM
                     GYCGAETIAAMQARARFVRITGAGLRESHPHDVTITKDSPNYRRL"
     gene            complement(1782..>2834)
                     /locus_tag="AC150V_10680"
     CDS             complement(1782..>2834)
                     /locus_tag="AC150V_10680"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIR3763423.1"
                     /translation="RRRERVKARKLGKGGLVRQENGPSPRRSGFLGTNVIVTVTLNAA
                     IDRTLTVPIFQLGHRHRASEALVLAGGKGINVARALKLLGVPVVATGLAGGRTGIRLV
                     EELTADAILNDFVRIQEESRTSTAVIDPTSGTHTEINEWGPRVTERELDMLREKLIYL
                     AGAAELVVLAGSLPRGVSEDFYADVIRELARSDVTVALDTEGEPLRLGLEAEPYLVTP
                     NQEEAEGVVGRELEDEEDFLMALDTLAERGARNVLLTLETGCYALFRIERQHQYFRAT
                     VPTLEGVSPLGSGDVLLAQFLAARRVEKPLDESLRDAVAAGAASVGEVGAGRFDPREA
                     NRLAEEVEVHELQPVS"
BASE COUNT          415 a         1002 c          988 g          429 t
ORIGIN      
        1 gggatcgacg cgcggggcgc tggccgcgta gacggatgcc ggcccgccgg acaggatgac
       61 agcgagcggg cggcgcgcac gaatcgcctc cgggctggtc ccgtgcccga cgagctccga
      121 atagacccgg gcctcacgga cgcggcgggc gatcagctgg ctgtactgcc cgccgaggtc
      181 gacgacgagc acgggtcgct cctcgggccg cggctcgggc agtgggacga cctcggcctc
      241 gctcacaggc gccggtagtt gggagagtcc ttggtgatcg tcacgtcgtg tgggtggctc
      301 tcgcgcagcc cggcgccggt gatgcgcacg aagcgggcgc gagcttgcat cgccgcgatc
      361 gtctcggcgc cgcagtagcc catcgcctgc cgcaggccgc cgacgagctg gtacacgatc
      421 ggcgccagcg gtcccttgta aggcacgcgc ccctcgatgc cttcggggac gagcttgtcg
      481 acgtcctcga cgtcgccctg gaagtagcgg tctttggaga acgagcgcgc cttcatcgcg
      541 ccgagcgagc ccatgccgcg gtactccttg aagcgctcgc cctggacgag cagcacctcg
      601 cccggcgact cgtccgtgcc tgcgagcatc gagccgagca tgacggtgtc ggcacccgcg
      661 gcgacggcct tggcgacgtc accggaggag gtcagcccac cgtcggcgac gatcgggacg
      721 ccgtagcgct gcgccacctc ggccgcatcg aagatcgccg tcacctgtgg catgccgaca
      781 ccggcgatga cgcgcgtcgt gcagatgctg cccggaccga caccgatctt gacgccgtcg
      841 gcgcccgcgt cgcagatcgc ctcggcggcc gcagcggtgg cgatgttgcc ggcgatgacc
      901 tgagtctccc acccgtcctt gatccggcgg acgacgtcga gcacgccctt ggagtgcccg
      961 tgcgccgtgt cgacgacgat cacgtcgact tccgcctccg cgagggcggc gacccggtcg
     1021 atcgcgtcgt tccccacgcc gaccgcggca ccgacgcgga gccggccccg ctcgtccttt
     1081 gaagcgagcg gatacttgac cttcttctga atgtccttga ccgtgatcag gcccttcaac
     1141 aggccgtccg cgtccacgac ggggagcttc tcgaccttgt ggcgccccag gatcgcctcg
     1201 gcctctgcga gcgtcgtccc caccggcgcc gtgacaagat cacggctcgt catgtaggcg
     1261 gagaccggct gggtcggatc ggcgccgaag cgcaggtcgc ggttagtgag aatcccgacg
     1321 agcacgcccg cgcgatccgt gatcggcacg ccggagacgc gatagcgggc catcagcgcc
     1381 agcgcgtcgg cgacgagcgc ctccggcggc agcgtgacgg gctcgacgat catgcccgcc
     1441 tcggagcgct tcaccttgtc gatctccgca acctgatcgc cgatcgagag gttgcggtgc
     1501 acgatgccga tcccgccctc gcgcgccagg gcgatcgcca gacgcgcctc ggtgaccgtg
     1561 tccatcgcgg ccgagacgac cgggatctcg atcgcgatcg tccgggtcag gcgcgtgacc
     1621 gtggagacgt cgttggggag caccgcggac tcggcgggca cgagcaggac gtcgtcgaag
     1681 gtcaggccct cttggccgaa cttcgcctcg cgatcgaaat cgagctccac gcggtggagt
     1741 gatcctacca gcgtgactcg acggcgacgg ccccggccgg gctatgagac gggctgcagc
     1801 tcgtgcacct cgacctcctc tgcgaggcgg ttggcctcgc gcgggtcgaa gcggccggcg
     1861 ccgacctcgc cgacgctcgc cgccccggcg gcgacggcgt cgcgcagcga ctcgtcgagc
     1921 ggcttctcca cgcgacgcgc ggcgaggaac tgggccagca gcacgtcgcc cgagccgagc
     1981 ggggagaccc cctcgagcgt cggcaccgtg gcgcggaagt actgatgctg gcgttcgatg
     2041 cggaacagcg cgtagcagcc agtctcgagc gtcagcagga cgttgcgcgc tcctcgctcc
     2101 gccagcgtgt cgagcgccat caggaagtcc tcctcgtcct cgagctcccg gccgacgact
     2161 ccctcggcct cttcctggtt cggcgtcacg aggtagggct cggcttcgag cccgaggcgg
     2221 agcggctcac cctccgtgtc gagggcgacg gtgacgtcgc tacgggccag ctcgcggatc
     2281 acgtctgcgt agaagtcttc gctcacccca cgcggcaacg agccggccag cacgacgagc
     2341 tcggcggcgc cggccagata gatgagcttt tcccgcagca tgtcgagctc gcgctcggtc
     2401 acccgcgggc cccactcgtt gatctcggtg tgcgtgcccg aggtcggatc gatcacggcg
     2461 gtggaggtac gcgactcctc ctggatgcgg acgaagtcat tgaggatcgc gtcggcggtg
     2521 agctcctcga cgaggcgtat gccggtccgg ccgcccgcga gaccggtcgc cacgacgggg
     2581 acgccgagca gcttcagcgc acgcgcgacg ttgatgccct tgccgccggc aagcacaagg
     2641 gcctcgctcg cccggtgccg atgaccgagc tggaagatcg ggacggtcag cgtccggtcg
     2701 atggcagcgt tcagggtgac ggtgacgatc acgttcgtcc ctaggaaacc agaccgacga
     2761 ggtgatgggc cgttctcttg gcgtaccagc ccacctttcc caagcttccg ggccttgaca
     2821 cgttctcggc ggcg
//
LOCUS       DBNGNK010000360         8353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_778365,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000360 DBNGNK010000000
VERSION     DBNGNK010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8353
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_778365"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="AC150V_10685"
     CDS             <1..496
                     /locus_tag="AC150V_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014165.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR3763424.1"
                     /translation="AVTRVRRTFVRVDADETSYGLHVILRFELEQEIIEGSVELADLP
                     EIWNARMKEFLGLDVPDDSHGVLQDVHWSGGGIGYFPTYALGNVISLQIWAAVRDALP
                     DVDDQIASGDFETLSLWLRDNLYSLGRKLTPKETLERVTGNGEIDPEPYLAYLRDKVL
                     ALAA"
     gene            493..915
                     /locus_tag="AC150V_10690"
     CDS             493..915
                     /locus_tag="AC150V_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763425.1"
                     /translation="MSVFREADSSAIRHALSFLEQDVPIELALGSGEEAVTVLVGARE
                     VDVGEETRKLVEAVTELSDRAVLTIDESPAEGRMLPATRIGEGLTYYGAPWGYELTTL
                     VGAIAECGRVDTPLSASSLAALAELDRDVALDVFVTPT"
     gene            928..1152
                     /locus_tag="AC150V_10695"
                     /pseudo
     CDS             928..1152
                     /locus_tag="AC150V_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024590.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
     gene            1163..2305
                     /locus_tag="AC150V_10700"
     CDS             1163..2305
                     /locus_tag="AC150V_10700"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR3763426.1"
                     /translation="MEPLERAQSLLDAYTLDLLAEMPAGFEAYDVHTHLGTDIDGMVG
                     ELDELLEIQQRVRISRSNVFCLDEPDRHPAFSAANDRTLGFAADAHGALVPFVRLDLN
                     ESPVEEAVRCLDAGARGIKLHPRAQAFLPDDPRLHPIFELAADRGVPILIHGGRGLPP
                     IAEGLAHLVDRYEPTLIIAHAGISDLAAMAHFFSGRPGVFFDTSVWSPIDLLDLYRLV
                     SPEQVLYASDYPYGRQPTSLLMTIRTARASGLEEQHVRAVLGESARRIADGEEALELT
                     PPRGRDSVEHPLTFARIHQYLSMASTLLWTGQGDTVGVLGLALNACDERSNGRREETE
                     RIRELLVATRDLWDLSAGAGNERLRSRTSRAAFRMLHLANVLAVTS"
     gene            complement(2500..3378)
                     /locus_tag="AC150V_10705"
     CDS             complement(2500..3378)
                     /locus_tag="AC150V_10705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR3763427.1"
                     /translation="MTTYARPATLEEALELLAEPTAAPLGGGTDLAGQIDRGIREPGV
                     LVDLQSLGLGEIFAREEGGVEIGATAALAEVAASAEVQAYESVVSAAAQAASPLLRNV
                     GTIAGNLCQHTRCWYYRGSEWNCWLGGGEICYAQIGDHRKHGLVAGECISAHPSDLAP
                     ALAACGAVVVVRSREGERELPLLQLYRTPTADNRSLLTLAPGELVVSIRLPSPPDRSI
                     YLRTGERRDFSFPLVSLAGARHGDTRRLVAAGVAPIPWELDSEDPLAELDGHPQSSWK
                     RDVLATLVVRAYEQLA"
     gene            complement(3375..5630)
                     /locus_tag="AC150V_10710"
     CDS             complement(3375..5630)
                     /locus_tag="AC150V_10710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR3763428.1"
                     /translation="MARLVRSQREMEGRFEDVWTLVDEEDDLETWPADEELALVGSPA
                     PRHDGIARAAGRVRYVVDVRLPGLLHAAVLRSPVAHARVENLDLQAALASPGVRAVLG
                     PDSEVGFEQRAPALVSEPEYAGQPIAVVAADSADHAEAGLRALALELEPLPHVVDLAE
                     ALREQRFTRDPQEETRGDVDAAFGEADVSVQLEIDTPDHLQTPLEPHAAVAVWDGSRL
                     TAWISTQGMFDARDGLAEAFGLARADVRVLCEYIGGAFGSKQGAGVEAILAAELARIT
                     DRPVRLVNDRHAEQLDGGRRARTRQTVRLGARRETGTLHAVEATAVIDQGRSGWVPPV
                     LVPALTLYRCDNVRAMTFPLKTNRRTQNAFRAPGVTEGTAVFEQAIDELAAALGLDPL
                     DFRRRNHVEVDQASGLPYSSKRLLACYDRAAELAGWEERAVLRRHHSDGRLRGMGCAT
                     QIWWGGGGPPAHATVRVDADARARVVTGIQDIGTGTLTAARIVAAEELGLPLEHVEVV
                     GGDTSHGVYGPVSGGSMTTPASLPPVRSAAAKVRKTLLELAAAVFEVAPDDLTIRGGR
                     VRSADGALDEALTEVTSKLGDATIDGSGSRGPNPDGVRVNTFGCQIAQIAVDPGLGEV
                     VVERVVAVHDVGRIINPLAAESQVEGGVLQGMAFALTEELVVDPTTGTPVNATLDDYK
                     LPTIADVPEIVVDFVDLPDPGLPNVGSRGLGEPPIVPTAAAIANAFADATGKRTKALP
                     LTRARVLEVLG"
     gene            complement(5630..6088)
                     /locus_tag="AC150V_10715"
     CDS             complement(5630..6088)
                     /locus_tag="AC150V_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR3763429.1"
                     /translation="MRITLDVNGGSYQVDVPAGRRLLAVLRDDLGLLGSKLACGEGSC
                     GACAVLLDDRPVASCLVVAPAAERHRIVTIEGLGADGATPLQEAFVAEDALQCGFCTP
                     GQIVTATALLARTPKPTREQIRSAMAGNLCRCGAYPKIERAILRAAETDG"
     gene            complement(6085..7122)
                     /locus_tag="AC150V_10720"
     CDS             complement(6085..7122)
                     /locus_tag="AC150V_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763430.1"
                     /translation="MSSDGPLVEVDDLRVWFPIKSGLVLDRHVGDVKAVDGVSLTIER
                     GETLGLVGESGCGKSTLGRTILRLYDPTSGRVVFDGTDITDLSERQLRPLRKRMQIVF
                     QDPFSSLNPRHSVGRMVAEPLRVHGVARGREAASRVRELLEVVGLPPDASARYPHEFS
                     GGQRQRIGLARALALNPEFLVCDEPVSALDVSIQAQIINLLEELQHELGLTYLFVAHD
                     LAVVQHISDRIAVMYLGKVVEVAPADDLYENPLHPYTITLLSSIPIPDPEIERQRRAI
                     RVEGDLPSPANPPDACRFHTRCPFAQPSRCADEEPLPRQVGGHLVACHYAEQIKAGEI
                     SARESAVVAPS"
     gene            complement(7119..8132)
                     /locus_tag="AC150V_10725"
     CDS             complement(7119..8132)
                     /locus_tag="AC150V_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763431.1"
                     /translation="MTAAPLLAVDDLRVEFSTTRGTVYAVNGASFHVAQGETLGLVGE
                     SGCGKSVTSLALLGILSPNGRVTAGTAMFESKDLLAQSDDELRQIRGKDIAMIFQDPM
                     TSMNPVLTVGRQIVEALETHLGLGGVEAQTRAAELLDQVGIPNARDRLRDYPHQFSGG
                     MRQRAMIAMALACEPKLLIADEPTTALDVTIQAQILDLLRELVADRETALLLITHDLG
                     VVSGMCERVHVMYAGTIVEKGSAAQVFAHPRHPYTLGLLQSVPRLDIGRRQALHPIEG
                     QPRSMLSPPTFCPFAPRCPRALERCTAELPRLEQLEAGQTAACFNVVPADAWQQIKLG
                     GVA"
     gene            complement(8129..>8353)
                     /locus_tag="AC150V_10730"
     CDS             complement(8129..>8353)
                     /locus_tag="AC150V_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease"
                     /protein_id="HIR3763432.1"
                     /translation="AIIDVAGLGFLGLGPQDPSTPEWGTMLTDTVRYLQTAPHLVFFP
                     GLAIVIVVLGFNLIGDGLREALDPKLGDNR"
BASE COUNT         1250 a         2896 c         2878 g         1329 t
ORIGIN      
        1 agcggtcacc cgcgtgcgcc ggacgttcgt ccgcgtcgac gccgacgaga cgagctacgg
       61 gctccacgtc atcctccgct tcgagctcga gcaggagatc atcgagggga gcgtcgaact
      121 ggcggatctg ccggagatct ggaacgcgcg aatgaaggag tttctcggcc tcgacgtccc
      181 ggacgactcc cacggagtcc tccaggacgt tcactggtcc ggcggtggaa tcggctactt
      241 cccgacctac gcactcggaa acgtgatctc gctccagatc tgggcggccg tccgggacgc
      301 actcccggac gtcgacgacc agatcgcgtc cggcgatttc gagacgctct cgctctggct
      361 gcgagacaac ctctattcat tgggccgcaa actgaccccg aaggagacgc tggaacgtgt
      421 gaccggaaac ggcgagatcg acccggagcc gtatctcgcc tatctccgtg acaaggtgct
      481 ggcgctcgct gcgtgagcgt gttccgggaa gccgactcga gcgcgatccg tcacgcgctg
      541 tctttcctcg agcaggacgt tccgatcgag cttgcgctcg gctccggcga ggaggcggtg
      601 acggtcctcg tcggcgcccg ggaggtcgac gtcggagaag agacgagaaa gctggtcgaa
      661 gccgtgaccg agctttctga ccgcgccgtc cttacgatcg acgagtcgcc ggcagagggg
      721 aggatgctgc ctgccacgcg catcggcgag ggtctcacct actatggcgc gccctgggga
      781 tacgagctca cgacgctcgt cggcgcaatt gcggaatgcg gccgggtcga cacgccgctg
      841 tccgcgtcgt cgctggcggc tctggccgag ctcgatcgcg acgtcgctct cgacgtcttc
      901 gtcaccccga cctgacccca ctgcccgccg gccgtgctgc tggccacgcg ttgcgcgctt
      961 gcctcggcac gggtctccgc ggcggcgatc gaggcgaccg cgtttccggg gcttgccggc
     1021 aggctcggcg tgctggaggt tccagccttc gccgtcgacg acacgctcgc ctggtcgggg
     1081 cgcctcggcg agtccgcctt cgtcgaacgc ctggtcgcgc tggccggttc cgattcggcg
     1141 acccccgcgt agactcaggt acgtggagcc gctcgagaga gcacagtcac tcctggacgc
     1201 gtacacgctc gacctgctcg ctgaaatgcc tgctgggttc gaggcgtacg acgtccacac
     1261 ccatctcggc accgacatcg acggcatggt cggcgagctg gacgagctgc tcgagatcca
     1321 gcagcgtgta cggatctccc ggtcgaacgt gttctgtctc gacgagcccg atcgtcaccc
     1381 ggcgttcagc gccgcgaacg accgcacgct cggcttcgcc gccgacgcac acggagcgct
     1441 cgtgccgttc gtcaggctcg acctcaacga gtcgccggtc gaggaggctg ttcgctgcct
     1501 ggacgcaggc gcccgcggga tcaagctcca tccgcgcgcg caggccttcc tgccggatga
     1561 tccgcggctg cacccgattt tcgagctcgc cgcggaccgc ggtgtcccga ttctcattca
     1621 cggtggacgc gggctccctc cgatcgccga gggattggcc catctggtcg accgttacga
     1681 gccgacgttg atcatcgccc acgcggggat ctcggacctc gctgcaatgg cccacttctt
     1741 cagcggccgg cccggtgtct tcttcgacac gtcggtgtgg agcccgatcg acctgctcga
     1801 tctgtaccgg ctcgtctcgc ccgaacaggt tctctacgcg tcggactacc cgtacggacg
     1861 gcagccgacg tcgttgctca tgacgatcag gacagcacgg gcgtccggcc tcgaggaaca
     1921 gcacgtgcgg gcggtcctcg gagagagcgc gcggcggatc gcggacgggg aggaggcgct
     1981 cgagctgacg ccgccgcggg gccgggacag cgtcgagcat cccctcacgt tcgcgcggat
     2041 ccatcagtac ctctcgatgg catctacgct cctctggacg ggacagggcg atacggtcgg
     2101 cgtcctcggt ctggcgctca acgcctgcga cgagcgctcc aacgggaggc gcgaggagac
     2161 ggaacggatc cgtgagctcc tcgtcgcaac gcgcgacctc tgggacctgt cggctggagc
     2221 gggcaacgag aggctccgct cccgtacgag tcgcgccgcg ttccggatgt tgcacctcgc
     2281 caacgtcctc gccgtcacct cctgacgccg ggtgctgttc atggctcgca cctggcccag
     2341 ccggggcttt gtcacggact cgcgccgctg gctccagccg cctttgcgtt cgacgctcct
     2401 gccgcgacgg cccttgtgga tgtttcgtca cacaggtcct cagaggtagg acgtggccgg
     2461 ttcggccctg gctccccggt ggagtcttag cgagtcgccc tacgccagct gctcgtaggc
     2521 acgcacgacc aacgtggcga gcacatcgcg cttccacgag ctctgggggt ggccgtcgag
     2581 ctcagcgagc gggtcttccg agtcgagctc ccacgggatg ggggcgacgc cggcggcgac
     2641 caggcgccgc gtgtccccgt ggcgcgcgcc ggcgagcgag acgagtggaa aggagaagtc
     2701 ccggcgctca cccgtgcgca gatagatcga gcggtcgggt ggcgaaggca gccggattga
     2761 cacgacgagc tcgccgggcg cgagagtcaa aagtgagcgg ttgtcggcgg tcggtgtgcg
     2821 gtacagctgg agcagcggca gctcgcgttc gccctcgcgt gaccggacga cgactaccgc
     2881 tccgcaggcg gccagcgcag gcgccaggtc ggaaggatga gccgagatgc actctcccgc
     2941 gaccaggccg tgcttgcggt ggtcgccgat ctgcgcatag cagatctccc cgcctccgag
     3001 ccagcagttc cactcgcttc cccggtaata ccagcagcgc gtgtgctggc ataggttccc
     3061 ggcgatcgtg ccgacgttgc ggagcagtgg cgaggccgcc tgcgccgcgg ccgagacgac
     3121 ggactcgtac gcctggacct cggcggaggc ggcgacctcg gcgagagcgg cggtggctcc
     3181 tatctcgaca ccgccttcct cccgtgcgaa aatttcgccg agcccgagcg actggaggtc
     3241 gacgagaacg cccggttcac ggatcccgcg gtcgatctgg ccggcgaggt cggtgccacc
     3301 gcctagcggc gcggctgtcg gctcggccag gagctcgagt gcctcttcga gtgtcgccgg
     3361 acgcgcgtac gtggtcaacc cagtacctcc agcacgcgcg ctctggtgag cggaagagct
     3421 ttcgtgcgct tcccggttgc gtccgcgaac gcgttcgcga tcgcggccgc ggtcggcacg
     3481 atcggcggct cgcccagccc tctcgaaccc acgttcggca ggccgggatc gggaagatcg
     3541 acgaagtcga cgacgatctc cgggacgtcg gcgatcgtag gcagtttgta gtcgtcgagc
     3601 gtcgcgttca cgggggtgcc ggtggtcggg tcgaccacga gttcttccgt cagcgcgaag
     3661 gccattcctt gcaggacgcc cccttcgacc tggctctcgg ccgccagcgg attgatgatc
     3721 cgaccgacgt cgtggacggc gaccacccgc tcgactacga cttccccgag gcccgggtcg
     3781 acggcgatct gcgcgatctg gcacccgaag gtgttcaccc ggacgccgtc cggattgggg
     3841 ccgcgcgagc ccgagccgtc gatcgtcgcg tctccgagct tcgacgtcac ctccgtcagc
     3901 gcctcgtcca gagcgccgtc ggccgagcgg acgcgaccgc ctctgatcgt gagatcgtct
     3961 ggcgcgacct cgaacaccgc cgcggcaagc tcgagcaacg tcttgcgcac cttggccgcc
     4021 gccgaacgca cgggtggaag cgacgccggt gtcgtcatcg agccccccga cacgggtccg
     4081 tagacgccgt ggctggtgtc cccgcccacc acctcgacgt gctcgagcgg cagccccaac
     4141 tcctccgcgg cgacgatccg cgcggccgtg agcgtgcccg tgccgatgtc ctggataccg
     4201 gtcacgactc gcgccctcgc gtccgcgtcg acccgcacgg ttgcgtgcgc cggcggcccg
     4261 cctccgcccc accagatctg cgttgcacag cccatgccgc gcaggcgccc gtcgctgtgg
     4321 tggcgtctga gcaccgcccg ctcctcccag cccgcgagct ctgcggctcg gtcgtagcag
     4381 gcgagcagtc gcttgctcga gtacggcaga ccgctcgcct ggtcgacctc gacgtgattc
     4441 cgccgccgga agtcgagcgg atccaggccg agggcggccg cgagctcgtc gatcgcctgc
     4501 tcgaagacgg cggtgccctc ggtcaccccc ggtgcgcgaa aggcgttctg cgtccggcgg
     4561 ttggtcttca gcggaaacgt catcgcccgc acgttgtcgc agcggtacag cgtcagtgcc
     4621 ggtacgagga ctggcggaac ccagccggag cggccctgat cgattaccgc ggtcgcctcg
     4681 acggcatgca acgttcccgt ctcccgccga gcgccgagac ggacggtctg ccgggtgcgc
     4741 gcccgcctgc cgccgtcgag ctgctcggcg tggcggtcgt tgacgagccg cacgggccgg
     4801 tcggtgatcc gggcgagctc ggctgcgagg atggcctcca cgccggcacc ctgcttcgag
     4861 ccgaaggcgc cgccgatgta ttcgcacagc actcgcacgt ctgccctggc gagcccgaaa
     4921 gcctcggcga ggccgtcgcg cgcgtcgaac atcccctgtg tcgagatcca cgcggtcagg
     4981 cgtgatccat cccagacggc cacggctgcg tgcggctcga gcggcgtctg caggtggtcg
     5041 ggtgtgtcga tctcgagctg caccgaaacg tccgcctccc cgaaagcggc atcgacgtca
     5101 ccgcgcgttt cctcctgcgg gtcgcgcgtg aaccgctgtt cccgcagcgc ctccgccagg
     5161 tcgacgacgt gcggaagcgg ctcgagctcg agcgcgagcg ctcgaagccc ggcttcggca
     5221 tggtcggccg agtctgccgc gacgaccgcg atgggctgcc ctgcgtactc gggctcggac
     5281 acgagcgcag gcgcacgttg ctcgaacccg acttccgagt cagggccgag gacagcccgc
     5341 acaccggggc ttgcaagcgc cgcctggaga tcgagattct cgacgcgcgc gtgcgcgacg
     5401 ggcgatcgga gaacggcggc gtgcagcagg cccggcagcc gcacatcgac gacgtagcgg
     5461 acacgacctg ccgctcgagc gatcccgtca tgccgtggcg ccggactgcc gacgagcgcg
     5521 agttcttcgt cagcgggcca ggtctcgaga tcatcctctt cgtcgacgag cgtccagacg
     5581 tcctcgaagc gaccttccat ctcccgttgt gagcggacga gtcgcgccat cagccgtccg
     5641 tctcagcggc gcggaggatc gcgcgctcga tcttcggata cgcgccgcag cggcagaggt
     5701 tgcccgccat tgccgaccgg atctgctcgc gcgtcggctt gggtgttcgg gcgagcaggg
     5761 ccgtcgccgt gacgatctgc cccggcgtgc agaagccgca ctggagagcg tcctcggcga
     5821 cgaatgcctc ttggagcggg gtcgcaccgt ccgccccaag tccttcgatc gtcacgattc
     5881 ggtgtcgttc tgccgcggga gcgacgacaa ggcacgatgc caccggtctg tcgtccagga
     5941 ggacggcgca cgcgccgcag gagccctcgc cgcacgcgag cttgctgccg agcaagccaa
     6001 ggtcgtcgcg cagcacggcg agcagccgtc gcccggcggg gacgtcgacc tgatagctgc
     6061 cgccgttgac gtcgagcgtg attctcacga cggtgcgaca acggcagact cgcgcgcgct
     6121 gatctccccg gctttgatct gctcagcgta gtggcacgcg acaagatgac ctcccacttg
     6181 ccgcgggagg ggctcctcgt cggcgcagcg cgacggctgc gcgaacgggc agcgcgtgtg
     6241 gaagcggcac gcgtcgggtg ggttcgcggg gctcggcaga tcgccctcga cccggatcgc
     6301 acgccgctgc cgctcgatct cggggtcggg gatcgggatc gaggagagga gcgtgatcgt
     6361 gtacgggtgg agcgggttct cgtacaggtc gtcggccggc gccacctcga cgaccttgcc
     6421 caggtacatg acggcgatcc ggtccgagat gtgctgcacg accgcgagat cgtgggccac
     6481 gaacaggtag gtgaggccga gctcgtgctg cagctcctcc aggaggttga tgatctgcgc
     6541 ctggatcgac acgtcgagcg ccgagacggg ctcgtcgcag acgaggaact cggggttcag
     6601 ggcgagagcg cgtgcgagcc cgatccgttg acgctgcccg cccgaaaact cgtgggggta
     6661 ccgcgcagag gcgtccggtg gcagcccgac cacctcgagc agctcgcgta cgcggctggc
     6721 tgcttccctg ccgcgtgcga cgccgtggac gcggagcggc tcggccacca tgcgtccgac
     6781 ggaatggcgg gggttgaggg acgagaacgg atcctggaag acgatctgca tccgcttgcg
     6841 cagcggccgc agctgccttt ccgagagatc cgtgatatcc gtgccgtcga agaccacccg
     6901 accgctggtc gggtcgtaga ggcgcaggat cgtgcggccc agagtcgact tgccgcaacc
     6961 cgactcgccg acgagtccca gcgtctcgcc gcgctcgatg gtcaaggaca cgccgtcgac
     7021 cgctttgacg tctccgacgt gccgatcgag cacgagcccg gacttgatcg gaaaccagac
     7081 ccgcaggtcg tcgacctcga cgagcggccc gtcgctgctc aagcgacccc gcccagcttg
     7141 atctgctgcc aggcgtcggc cggcaccacg ttgaagcagg ccgccgtctg acctgcttcg
     7201 agctgctcga gcctgggcag ctccgcggtg caccgttcga gtgcacgagg gcagcgcggc
     7261 gcgaaggggc agaaggtcgg cgggctcagc atgctccgag gctgtccctc gatcgggtgc
     7321 agcgcctgac gccggccgat gtcgagcctg gggacgctct ggagcagccc gagcgtgtag
     7381 gggtgacgcg gatgggcgaa cacctgcgcc gccgaccctt tctcgacgat cgtgcccgcg
     7441 tacatgacgt ggacgcgctc gcacatgccc gagacgacgc cgaggtcgtg ggtgatcagc
     7501 agcagcgccg tctcgcggtc ggcgacgagc tcacggagga gatcgaggat ctgtgcctgg
     7561 atcgtcacgt cgagggccgt cgtcggctcg tcggcgatca gcagcttcgg ctcacaggcg
     7621 agcgccatgg cgatcatcgc ccgctggcgc atgccgcccg agaactgatg cggatagtcg
     7681 cggagccggt ctcgcgcgtt cgggatgccg acctggtcga gcagctccgc cgccctcgtc
     7741 tgagcctcca ctccgcccag gcccaggtgc gtctccaacg cctcgacgat ctgacgcccc
     7801 acggtgagca ctggattcat ggacgtcatc gggtcttgga agatcatcgc gatgtccttc
     7861 ccgcggatct gtcgcaactc gtcgtccgat tgcgcgagca aatccttgct ctcgaacatc
     7921 gcggtccccg ctgtcacgcg cccgttcggg gacaggatcc cgagcagggc gagcgaggtg
     7981 acgctcttgc cgcagcccga ctcgcccacg agccccagcg tctcgccctg tgcgacgtgg
     8041 aacgaggctc cgttgaccgc gtagacggtg ccccgcgtcg tcgagaactc gacgcgcaga
     8101 tcgtcgaccg cgagcagtgg cgctgcggtc accgattgtc cccgagcttc ggatcgagag
     8161 cttcgcgcag tccgtcgccg atgaggttga agccgagcac gacgatgacg atcgcgaggc
     8221 cggggaagaa gacgaggtgc ggcgcagtct gcaggtagcg caccgtgtcg gtgagcatcg
     8281 tgccccactc cggggtcgac ggatcctgcg ggccgagccc gaggaagccg aggcccgcca
     8341 cgtcgatgat cgc
//
LOCUS       DBNGNK010000361         3841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_851725,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000361 DBNGNK010000000
VERSION     DBNGNK010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_851725"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /gene="ruvB"
                     /locus_tag="AC150V_10735"
     CDS             <1..779
                     /gene="ruvB"
                     /locus_tag="AC150V_10735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR3763433.1"
                     /translation="DVGLRAIAGPALERKGDMAAILTGLEARDVLFIDEIHRVNRAIE
                     EILYPALEDFRLDIVVGQGPAARTLTLDLPPFTLVGATTRTGLLTTPLRDRFGLTFRL
                     GYYDADQLTAIVRRSADILDVPIDDDAAREISRRARGTPRVANRILRRVRDVAEVRHD
                     GEITAPVAHEALELLEVDGEGLERIDRELLAAIAHKFEGGPVGLSTLAVALGEEPDTI
                     EEVYEPYLLQLGFLQRTPRGRVITELGRAHVGTTPAERLF"
     gene            812..1129
                     /locus_tag="AC150V_10740"
     CDS             812..1129
                     /locus_tag="AC150V_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763434.1"
                     /translation="MSTLLWTPGAAGPLDELIKRISRMVEAFATEYKLEQAEVRVELA
                     DGSHHQLAAISPEPGFGFFSFTPHAPDGEEPRRMVVPIGAVKFIEVSVPDPERPFGFS
                     ADG"
     gene            complement(1241..1699)
                     /locus_tag="AC150V_10745"
     CDS             complement(1241..1699)
                     /locus_tag="AC150V_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763435.1"
                     /translation="MPAEANYSSGDDYVVEFLGYRFGFAARDFEERVVSAAVRLGLVE
                     TNDLDGEEVGDLVELTADGRIAEARSGLGQYLVRHWERLALVDGESLVYWLRKLVFRG
                     AWLDHRVKEGLLDVSWDEDSGDFGYAEPKGGRALLELAPVPSWHALQFRR"
     gene            1723..1941
                     /locus_tag="AC150V_10750"
     CDS             1723..1941
                     /locus_tag="AC150V_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763436.1"
                     /translation="MRDKRLIAAMIGVVIAIWLIGAAITKYSLEQMAYYMPIAVVVIG
                     LTVGIVLLWAKVITEALRGRRGGREQRG"
     gene            2003..>3841
                     /gene="recJ"
                     /locus_tag="AC150V_10755"
     CDS             2003..>3841
                     /gene="recJ"
                     /locus_tag="AC150V_10755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR3763437.1"
                     /translation="MQDGRWTLRPCPYREVVALARELGISEITASVLVRRGYGDPSNA
                     ERFLAAGDPGHDPRLLGDMVSAVELLRRAAADGTAICVHGDYDVDGISATALAVLTLR
                     ELGADVRWHLPSRFEEGYGVSADTVAKLAAEGVGLLVTVDCGITAVDEIELAKSLGLA
                     VVVTEHHRPAERLPDCPVVATRPSDYPFPELCGTGVVHKLAQALLGREHPAVRSHLDL
                     VALATIADVVPLLDENRALAAAGLRALACTQKRGLQALMRTSGVDPATIDSGAVAFRL
                     APRINAAGRLGRPDLALELILSDDPAEAGRLAGELEMLNRERQAIEDRIQREAVKLVE
                     SWPDESRARRGYVLAAEDWHEGVIGIVASRLVERFHRPVVMIAGGEGEWKGSGRSIPS
                     FDLHNALSATSGHLERFGGHRAAAGLTISRDRVDGFAHAFAAHAATMLSDEDLSRTTP
                     IDAVVPATALTLGLAQELNRLAPFGLGNPDVTLLVAGCELTDVTPVGEGKHLRFRVRQ
                     DARDAGSAIAFGMGRELDRLEPGSSYDVAFRLKENRWNGTVAPQLVVRRIFESHEGYA
                     ALRARLAELWRAGESAWTPEARQIFAELALEPGGGKRQLLESETFRA"
BASE COUNT          568 a         1330 c         1345 g          598 t
ORIGIN      
        1 tggacgtggg gctccgggcg attgccgggc ccgctctcga gcgcaaagga gacatggccg
       61 cgatcctcac ggggctcgag gcgagagacg tgctcttcat cgatgagatc caccgggtca
      121 accgtgcgat cgaggagatc ctgtacccgg cgctggagga cttccgtctc gacatcgtcg
      181 tggggcaggg gcctgcagcg cgcacgctga cgctcgacct ccctccgttc accctcgtcg
      241 gcgcgacgac ccggacgggc cttctgacga cgccgctgcg cgaccggttc gggctcacat
      301 tccgtctcgg ctactacgac gccgatcagc tcaccgcgat tgtccgccgc tcggcggaca
      361 tcctggacgt gcccatcgac gacgacgccg cgcgcgagat ctcgcgtcgt gcgcgcggga
      421 cgccccgggt cgcgaacagg attctccggc gcgtccgtga cgtggccgag gttcggcatg
      481 acggcgagat cacggccccg gtggcacatg aggcgctcga gctgctggag gtcgacggcg
      541 aagggctgga gcgcatcgac agggagctgc tggctgcgat tgcccacaag ttcgagggcg
      601 gcccggtcgg gctctccacg cttgcagttg cactcggcga ggagcccgac acgatcgagg
      661 aggtctacga gccctacctt ctccaactcg gcttcctgca gcgcacaccg cgcggacgag
      721 tcatcacgga gctcgggcgg gcccacgtcg ggacgacgcc ggctgagcgg ctcttctgag
      781 cccaagtcgc agcgtcgcgt aagctctggg cgtgtcgacg ctgttgtgga cacccggcgc
      841 cgccggaccg ctcgacgagc tgatcaagcg gatctcgcgg atggtggaag ccttcgccac
      901 cgagtacaag ctcgagcagg cggaggtgcg cgtcgagctc gccgacgggt cgcaccatca
      961 gctcgcggcc atctcgccgg agccgggttt cggatttttc agcttcacgc cgcatgcgcc
     1021 cgacggagag gagccgcggc gcatggtcgt gccgatcggc gcggtcaagt tcatcgaggt
     1081 ctcggtaccc gatcccgaac ggcctttcgg gttctcggcg gacggctgac gtcgcccgtc
     1141 ggctgcgtcc ggaagctgtg ctgtttcgtg gcagctcggt aacagcggtc ctcagaggcg
     1201 gggcgtggcc gtttcggcct cggcctgcgg tgtctcggct ctagcgcctg aattgcagag
     1261 cgtgccagct cggaacgggc gcgagctcga ggagtgcgcg gccgcccttg ggctccgcgt
     1321 acccgaagtc cccggaatcc tcgtcccagg agacgtcgag gagaccctcc ttgacgcggt
     1381 gatcgagcca cgcgccgcgg aagacgagct tgcggagcca gtagacgaga gactcgccgt
     1441 cgacgagggc gagtctctcc cagtggcgta cgagatactg gccgaggccg ctgcgcgcct
     1501 cggcgatccg gccgtccgcc gtgagctcga cgaggtcgcc cacctcttcg ccgtcgagat
     1561 cgttcgtctc gacgagcccc agccgcacgg ccgccgagac gacgcgctcc tcgaaatcgc
     1621 gggccgcgaa gccaaaccgg tagccgagga actcgaccac gtagtcgtcg ccggagctgt
     1681 agttggcctc cgctggcacg cgcccaaggg tagcctcacg gtatgaggga caagcgtctc
     1741 atcgcggcga tgatcggggt cgtgatcgcg atctggctca tcggtgcggc gatcacgaag
     1801 tactcgctcg agcagatggc ctactacatg ccgatcgcgg tcgtcgtgat cgggctgacc
     1861 gtgggcatcg tgctgctctg ggcgaaggtc atcaccgagg cgctgcgtgg ccgccgaggc
     1921 ggccgcgagc agcgcgggta gtcgcccgac gcatcggacg caggcccact ccgtccgaca
     1981 ctccccgtag cctcgaagca cgatgcagga cggacggtgg actctccgtc cgtgcccgta
     2041 ccgagaggtt gtcgcgctgg cgcgggagct cgggatcagc gagatcaccg cgagcgtcct
     2101 cgtccggcgc gggtacggcg acccctcgaa cgcggagcgc tttctggccg ccggcgaccc
     2161 agggcacgat cctcgtctgc tcggcgacat ggtttcggcg gtcgagctgc tccgccgggc
     2221 ggcggcggac gggacggcga tctgcgtgca cggcgactac gacgtggacg gcatcagcgc
     2281 gaccgcgctc gccgtcctga ctctgcggga gctcggcgcg gacgtccgct ggcacctgcc
     2341 gagccggttc gaggagggct acggcgtctc ggcggacacc gtcgccaagc tcgccgccga
     2401 aggggtcggc cttctcgtca ccgtcgactg cggcatcacc gccgtcgacg agatcgagct
     2461 cgcgaagtcg ctcgggctcg ccgttgtcgt cacggagcat cacaggccgg cggagcgcct
     2521 cccggactgc cccgtcgtcg cgacccggcc gtccgactat ccgttcccgg agctctgcgg
     2581 cacaggcgtc gtccacaagc tcgcgcaggc gctcctcggc cgggagcatc cggccgtcag
     2641 gagccatctc gatctcgtcg ccctcgcgac catcgcggac gtcgtgccgc tcctcgacga
     2701 gaaccgcgcg ctcgcggcgg ccggactgcg tgcgctcgcg tgcacccaga agcgcggctt
     2761 gcaagcgctg atgcggacga gcggggtcga tccagcgact atcgacagcg gcgcagtcgc
     2821 cttccgcctg gcgccccgga tcaacgccgc cggcagactc ggccgtcctg atctcgccct
     2881 cgagctcatc ctcagcgacg accccgcgga ggccggccgc ctcgcgggcg aactcgagat
     2941 gctcaaccgt gagcgacagg ccatcgagga caggatccag cgcgaggcgg tcaagctcgt
     3001 cgagtcctgg ccggatgaga gccgcgcccg ccgcggatat gtcctcgccg cagaggactg
     3061 gcacgagggg gtgatcggca tcgtcgcctc gcgcctcgtc gagcgctttc accgccccgt
     3121 ggtgatgatc gctggcggcg agggagagtg gaagggctcc ggtcgctcga tcccgtcttt
     3181 cgacctccac aacgcgctct ccgccacgag cgggcatctg gagcgcttcg gcgggcatcg
     3241 agcggcggcc ggcctgacga tcagccgcga ccgggtcgac gggttcgccc acgccttcgc
     3301 cgctcatgcg gcgaccatgc tctccgacga ggatctgagc cgcacgactc cgatcgacgc
     3361 cgtggtgccc gcgaccgcgt tgacactcgg gcttgcccag gagctcaatc gcctcgcgcc
     3421 gttcgggctc ggcaatcccg acgtcactct gctcgttgcg ggctgtgagc tcaccgacgt
     3481 tacgccggtc ggagagggta agcatctccg ttttcgcgta cggcaggacg ctcgcgacgc
     3541 agggagcgcg atcgccttcg gaatgggtcg ggagctcgac cggctcgagc ccggctcgag
     3601 ctacgacgtt gccttccgcc tgaaagagaa ccggtggaac ggaacggttg cgccgcagct
     3661 cgtcgttcgc aggatcttcg agtcccacga gggctacgcg gcgcttcgcg ccagactggc
     3721 ggagctctgg cgggcgggcg agtcggcctg gactccggag gcgcgccaga tcttcgccga
     3781 gctggcgctg gaaccgggcg gcggcaagcg tcagctgctc gagtccgaga ccttcagagc
     3841 g
//
LOCUS       DBNGNK010000362         3867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1782186,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000362 DBNGNK010000000
VERSION     DBNGNK010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1782186"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="AC150V_10760"
     CDS             <1..971
                     /locus_tag="AC150V_10760"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR3763438.1"
                     /translation="ADVLAMAPQTNIGSSTPISATGEELGEDLRRKIVNDAAAYTAEL
                     AREHGRNAQAAEAMVRTAANFGAREALEKNVVDVVAPDVPTLLKELDGTTTVPKGLVL
                     NTAGADVERVQMSLWKSLLDLLIDPNLIAIMLSIGLIGIVVELWNPGLIFPGTVGAIS
                     LIVGLYGLQVLPVSIAGLLLMALAAAFFVAEAFVPSSGALTVAGAVMFVIGALMLFDP
                     AGEAYQVSLWVAIGIAVALALIVGVALSRVARSARRPVEVGLDRFVGEEGEIRRDGLV
                     FLEGELWRARSRDGEPLVPGERVRVEEVDDALELVVVGSSQSTEGS"
     gene            980..1777
                     /locus_tag="AC150V_10765"
     CDS             980..1777
                     /locus_tag="AC150V_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR3763439.1"
                     /translation="MSGALVALAAVVIVIVILLFAAIKVAREYERAIVFRLGRLLPEP
                     KGPGLFILIPIIDRMVKTDLRTVTLNVPPQEVITKDNVPVRVNAVAYFRIVEPKASVV
                     QVENFMAATSQISQTTLRSVLGQHMLDELLSERDKINAILQSIIDEATSPWGIKVSIV
                     EVKDVEIPGNMQRAMARQAEAERERRAKVIAAEGEYQASERLRDAAAVMSEESIALQL
                     RYLQTLVEIGASNNSTVVFPLPIDIVKPLLDLGAGNSKREIGEDSGG"
     gene            complement(1774..>3867)
                     /locus_tag="AC150V_10770"
     CDS             complement(1774..>3867)
                     /locus_tag="AC150V_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR3763440.1"
                     /translation="APNSAASRREPTVLPSRFPNLLVNGSAGIAVGMATNMPPHSLRE
                     TADAIVELIDKPDASVDDLMRHLKGPDFPTGAIVVGRSGIRDAYRTGRGRIVMRARAH
                     VEELRGGKSAIIVTELPYGVKKGGDSGVIHKIADLVREKVITEISDLQDYSDRTGMRI
                     QVELKREAIPQVVLNKLFKHTALQTTFGYNAVALADGVPRTLSLLELVQHYLDYQREV
                     VTRRSKYELRKAVERAHVLEGYLIALDNLDAVIDLIRGSEDTDAARHALMAQFSLSEV
                     QAQAILDLRLARLTGLARKEIEDEFADLQERIGELRAILGDPARIDGVIREELLEIKE
                     IYARDDERRTEIVAAEDELELEDLIAEEDMVIAITQSGYIKRLPVTAYREQRRGGIGV
                     MGMELKDGDYIEHLFVASTHDYILFFTTVGKVYRLKVHELPLGSRQAKGRAIVNLLPL
                     RQDEAVRAVIATRNFEEAKYLVFATKGGVVKKTEFKDYNTPLRADGIIAIKLREGDEL
                     IGVRLSDGQDDVLMVSRLGQAVRFHETDVRPMGRPASGVAGMKLRKGDEVIAVDIAED
                     DADLLVVTENGWGKRTRLTEYPVKGRGTMGVKTARLVEGKGRLVGARIVRDGYQMMLI
                     SDAGTVIRIPVEDVKRAGRATQGVIVMRPRDGEQVSALAPVTSADEDEPVADDPAADE
                     PATSEPDAGEPDAGD"
BASE COUNT          573 a         1356 c         1252 g          686 t
ORIGIN      
        1 cggcggatgt cctcgcgatg gcgccgcaga cgaacatcgg ctcgtcgacg ccgatctccg
       61 ccaccggtga ggagctcggc gaggatctcc gcagaaagat cgtcaacgat gccgcggcgt
      121 acacggccga gctcgcccgg gagcacggcc gcaatgcgca ggcggccgag gcgatggtcc
      181 gcaccgcggc gaacttcggc gcacgcgagg cgctcgagaa gaacgtcgtc gacgtcgtcg
      241 cgccggacgt ccccactctc ctgaaagagc tcgacgggac aacgaccgtt cccaagggcc
      301 tcgtgctgaa caccgccggg gcagacgtgg agcgggttca gatgtcgctc tggaagtcgc
      361 tgctcgacct gctgatcgat cccaacctga tcgccatcat gctctcgatc gggctgatcg
      421 gcatcgtcgt cgagctgtgg aacccgggtc tcatcttccc gggcacagtc ggggcgatct
      481 cgctcatcgt cggcctctac ggcctccagg ttctcccggt ctcgatcgcc gggctgctcc
      541 tgatggcgct ggccgccgcc ttcttcgtgg ccgaggcctt cgtcccctcc agcggggccc
      601 tgaccgtcgc cggggcggtg atgttcgtga tcggcgcgct catgctcttc gacccggccg
      661 gtgaggcgta tcaggtatcc ctgtgggtcg cgatcggcat cgcggttgct ctggcgctca
      721 tcgtcggcgt tgccttgtca cgcgtcgccc gctccgcccg gcgcccggtc gaggtcggcc
      781 tcgatcgctt cgtgggcgag gagggcgaga tccgccgtga cggactcgtc tttctcgagg
      841 gcgagctctg gcgcgctcgc tcgcgcgacg gggaaccgct cgtgccaggc gagcgcgtcc
      901 gcgtcgagga agtggacgac gcgctcgagc tcgtcgtcgt aggatcgtcg caatcaaccg
      961 aagggagctg aagcccgctg tgtccggagc actcgtagcc cttgccgcag tcgtcatcgt
     1021 catcgtcatc ctgctcttcg cggcgatcaa ggttgcccgc gagtacgaac gggcgatcgt
     1081 cttccgcctc ggtcgcctcc tgcccgagcc caaggggccg ggcctcttca tcctgatccc
     1141 catcatcgac cgaatggtga agaccgatct gcgcacagta accctgaacg tgccgccgca
     1201 agaggtaatc acgaaggaca acgtccccgt gcgggtgaac gcggttgcct acttccgcat
     1261 agtcgagccg aaggcgtccg tcgtgcaggt ggagaacttc atggccgcca cctcgcagat
     1321 ctcccagacg accctccgct cggtgctcgg tcagcacatg ctcgacgagc tgctctctga
     1381 gcgcgacaag atcaacgcga tcctgcagtc gatcatcgac gaggccacct ctccctgggg
     1441 catcaaggtc tcgatcgtcg aggtgaagga cgtcgagatc ccgggcaaca tgcagcgcgc
     1501 gatggcgcgc caggccgagg ccgagcgcga gcggcgtgcg aaggtcatcg ccgccgaggg
     1561 tgagtaccag gcctccgaga ggctacgcga cgccgccgcg gtcatgtcgg aggagtcgat
     1621 cgcccttcag ctgcgttacc tgcagacgct cgtcgagatc ggcgcctcga acaactccac
     1681 ggtcgtcttt ccgcttccga tcgacatcgt caagccgctc ctcgacctgg gcgccggcaa
     1741 ctcgaagcga gagatcggag aggactccgg gggctagtcg cccgcgtcag gctcgcccgc
     1801 gtcaggctcg ctcgtggcgg gctcgtccgc cgcggggtcg tccgcgaccg gctcgtcttc
     1861 gtcggcgctc gtcacgggcg cgagcgccga gacctgctcg ccgtcgcgtg gccgcatcac
     1921 gatcacgccc tgggtcgcgc gcccggcgcg cttcacgtcc tcgacgggga tgcggatgac
     1981 ggtgcctgcg tccgagatga gcatcatctg gtagccgtcc cgcacgatgc gggcgccgac
     2041 cagccgcccc ttcccctcga cgagcctcgc cgtcttgacg cccatcgtgc cgcgaccctt
     2101 gaccgggtac tccgtcagcc gcgtccgctt cccccagccg ttctcggtca cgacgagcag
     2161 gtcggcgtcg tcctcggcga tatcgacggc gatcacctcg tcgcccttgc gcagcttcat
     2221 ccccgcgacg ccggaggcgg gcctccccat cggccgcacg tccgtctcgt ggaaccggac
     2281 cgcctgaccg aggcgggaga ccatcagcac gtcgtcctgg ccgtcggaga ggcgaacgcc
     2341 gatcagctcg tcgccctcgc gaagcttgat cgcgatgatc ccatcggccc gaagtggcgt
     2401 gttgtagtcc ttgaactccg tcttcttgac gacgccgccc ttggttgcga agacgaggta
     2461 cttggcctcc tcgaagttgc gtgtcgcgat gacggcccgc acggcctcgt cctggcgcag
     2521 cggcaggagg ttcacgatcg ccctcccctt cgcctggcgc gagccgagcg gcaactcgtg
     2581 caccttcagc cggtacacct ttcccacggt cgtgaagaag aggatgtagt cgtgtgtcga
     2641 cgcgacgaag agatgctcga tgtagtcgcc gtccttgagc tccattccca tcacgccgat
     2701 cccgccgcgg cgctgctccc ggtaggcggt cacgggtagc cgcttgatgt agcccgactg
     2761 ggtgatcgca atgaccatgt cctcctcggc gatcagatcc tcgagctcga gctcgtcctc
     2821 cgcggcgacg atctccgtgc gccgctcgtc gtcgcgcgcg tagatctcct tgatctccag
     2881 cagctcctcg cggatcacgc cgtcgatgcg cgcaggatca ccaaggatcg cgcgcagctc
     2941 tccgatgcgc tcctgcagat cggcgaactc gtcctcgatc tccttgcgcg ccagtcccgt
     3001 cagccgcgcg agccgcaggt cgaggattgc ctgcgcctga acctcggaca gcgaaaactg
     3061 cgccatcagc gcgtggcgcg ccgcgtcggt gtcttccgat cctctgatca ggtcgatcac
     3121 ggcgtcgaga ttgtcgagag cgatcaggta gccctcgagc acgtgcgcgc gctcgaccgc
     3181 cttgcgcagc tcgtacttcg agcgtcgggt gacgacctcg cgctgatagt cgaggtagtg
     3241 ctgcaccagc tcgagcagag acagcgttcg cggcaccccg tcggcgagcg ccaccgcgtt
     3301 gtagccgaag gtcgtctgca gcgccgtgtg cttgaacagc ttgttgagga cgacttgcgg
     3361 aatcgcctcg cgcttcagct cgacctggat gcgcatgcca gtccggtccg agtagtcctg
     3421 caagtcggag atctcggtga tcaccttctc gcggaccagg tcggcgatct tgtggatcac
     3481 gccgctgtcg ccgcccttct tcacaccgta gggcaactcg gtgacgatga tggcgctctt
     3541 ccccccgcgg agctcctcga cgtgagccct ggcgcgcatg acgatccgcc cgcgtcccgt
     3601 gcggtaggcg tcccggatgc cggagcgccc gacgacgatc gctccggtcg ggaagtccgg
     3661 ccccttcaga tgacgcatca ggtcgtcgac gctcgcgtcg ggcttgtcga tcagctcgac
     3721 gatcgcgtcg gctgtctcgc gcaggctgtg cggcggcatg ttcgtcgcca tcccgaccgc
     3781 gatccccgcg gagccgttga ccaagaggtt cgggaagcgc gagggcagca ccgtcggctc
     3841 gcgccgcgac gcggcggagt tcggcgc
//
LOCUS       DBNGNK010000363         4553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_135022,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000363 DBNGNK010000000
VERSION     DBNGNK010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_135022"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /gene="eno"
                     /locus_tag="AC150V_10775"
     CDS             complement(<1..326)
                     /gene="eno"
                     /locus_tag="AC150V_10775"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969226.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR3763441.1"
                     /translation="MTAILEIRARQVLDSRGNPTVEVDVGLESGALGRALVPSGASTG
                     EHEAVELRDGGEPWLGKGVQRAVDHVNGEITDRLVGLDADEQRAVDVALVELDGTPTK
                     SRLGANA"
     gene            complement(340..1179)
                     /gene="mazG"
                     /locus_tag="AC150V_10780"
     CDS             complement(340..1179)
                     /gene="mazG"
                     /locus_tag="AC150V_10780"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR3763442.1"
                     /translation="MASADDTASERGLARALLELQELTHTLRRECPWDRQQTARTIVP
                     HTVEEAYEVADAATSGSAEELHDELGDLLFQVYFLALLLEERGEGDLGTIARSVRDKL
                     VRRHPHVFGDADVRTAGRVRERWEEIKTEQEGRSGVFHEIPDALPALLHARKVQRRAA
                     AVGYDWPDVDGPLAKLHEELEELADATTAAGPPAPESESDPGVFAEVGDVLFTVVNLA
                     RRLNVDPELALRATTHRFVRRVQEAERLAAEAGETWSELDLEAQDGYYEQAKVRLAPN
                     GDQ"
     gene            complement(1230..2255)
                     /locus_tag="AC150V_10785"
     CDS             complement(1230..2255)
                     /locus_tag="AC150V_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR3763443.1"
                     /translation="MTRRLLLSVSVLGLLAVLVAAGCGGSSGESVPEGGVASVDGVVI
                     TKEQLDELLARAKVSYEAQQQDFPKVGTSEYQALQTQATSFLVQRAQYDQKAEELGIA
                     VTDKEVDDRVAEVKEEFFGGNEKEFSKQLKQQGYTDESFRSDIHAQLVTDKLFEEVTK
                     GLKVTDADAKQYYNENKAQFDVPESRDVRHILVKTKKQANGLYSELKAGADFAALAKQ
                     HSLDPGSKDAGGQLTVTRGQTVKPFDTTAFLLPKDALSRPVKTEFGYHLIQPLSEVKP
                     ARTTPYKEVKAQIKQQLRDQKTNAAVTAWASDLQTEFEDKTAYGEGYAPPDTGGAEED
                     APAPSGG"
     gene            complement(2365..>4553)
                     /gene="mfd"
                     /locus_tag="AC150V_10790"
     CDS             complement(2365..>4553)
                     /gene="mfd"
                     /locus_tag="AC150V_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR3763444.1"
                     /translation="MLRKVEGTLLEPGEPLPREPSLRFAVAPARRGFVWRELGLVLMP
                     ETQVFRKRPPRADPRLGRSLASFADLRSGDFVVHEDHGVGKLLGFETREVAAVTRDYL
                     LVAFKGDDRLYLPHEQLGKLSKYVGADGSAPALSKLGGKAWQNLKARARASVRELAGE
                     LLSLYAQRQRAPGVAYDLENDLLEQLEASFPYRETQDQERAIEIVKEDLEAARPMDRL
                     VCGDVGFGKTEVAVRAAFAVAANSRQTLFLCPTTILAEQHWNTFRERFRDLPVTVEMV
                     SRFRRTAEVKSVLAEFSSGKVDVLIGTHRVLSRDVIPKNLGLVILDEEQRFGVAQKEL
                     LRSLRLEVDVLALSATPIPRTLHMSLSGLRDISIIETPPEGRRAIRTTVGEYDEELIT
                     AALMREHGRGGQSFYLHNRVETIEEAAAKLQQLCPELRFLVAHGQMAERNLEERMHSF
                     LAGDGDVLVSTTIIESGIDIPQANTLIVERADQLGLSQLYQIRGRVGRSDVTAHAYLF
                     YPDASELTPEARARLATLADHTELGAGFQIAMRDLEIRGAGDLLGAEQSGHVAALGFE
                     LYVEMLGEAVAELTGQARVVARPVRVDARVDAFVPASYIESEALKIDLHRRLALVESD
                     DELRELEAATEDRYGPLPEPVGNLFAIQEAKLKLARLGADYLVLRGGRATVGPIPLAS
                     HEIRALRAISETAVYSTAKREVSRRSDDLRGALELADAIVDARLAA"
BASE COUNT          694 a         1551 c         1528 g          780 t
ORIGIN      
        1 gcattcgcgc ccaggcgact cttcgtaggc gttccgtcga gctcgacgag agcgacgtcg
       61 acagcacgct gctcgtccgc gtcgagaccg acgagccggt ccgtgatctc gccgttcacg
      121 tgatcgacgg cgcgttgcac gcccttgcct agccagggct cgccgccgtc ccgaagctcg
      181 acggcctcgt gctcgccggt cgacgctccc gagggaacga gcgctcgccc gagcgcgcca
      241 gactcgagac cgacatcgac ctcgacggtc gggttaccgc gcgaatcgag cacctggcga
      301 gcgcggatct cgagaatcgc ggtcacagcg ctccgcagac tactggtcac cgttcggcgc
      361 gagtcgcacc ttcgcctgct cgtagtagcc gtcctgcgcc tcgaggtcga gctcgctcca
      421 cgtctcgccg gcctcggcag ccagccgctc ggcctcttgg acacggcgca cgaaccgatg
      481 cgtcgtggcc cggagcgcca gctcagggtc gacgttgagc ctacgcgcga gattcacgac
      541 ggtgaagagc acgtctccga cctcggcgaa cacacccggg tcggattcgc tctccggcgc
      601 tggcgggccc gcagcggtcg tcgcgtccgc cagctcctcc agctcctcgt ggagcttcgc
      661 cagtgggccg tcgacgtcgg gccagtcgta gccgaccgcg gccgcgcgac gctgcacctt
      721 gcgggcgtgc agaagcgcgg gcaacgcgtc cgggatctcg tggaagacac cgcttcgccc
      781 ctcctgctcg gtcttgatct cctcccagcg ctcacggacg cggccggcgg tgcggacatc
      841 cgcgtcaccg aagacatggg gatgccgacg cacgagcttg tcccggacgc tgcgggcgat
      901 cgtcccgaga tcaccctcgc cgcgctcctc gagcaggagc gccagaaagt agacctggaa
      961 aaggagatcg ccgagctcgt cgtgtagctc ctccgcggag cctgaggtcg ccgcgtcggc
     1021 cacttcgtag gcctcttcga cagtgtgcgg gacgatggtg cgggccgtct gctggcggtc
     1081 ccaggggcac tcccgccgca gcgtgtgggt cagctcctgc agctcgagca gcgcccgagc
     1141 gagtccgcgc tcgctagccg tgtcgtcagc ggaggccaga gggcaatgct atccggggcg
     1201 ctcgggctca cccagacgga aagggaggat tagccgccgg acggggcggg agcgtcttcc
     1261 tccgcgccgc cggtgtccgg cggtgcatag ccctcgccgt acgccgtctt gtcctcgaac
     1321 tcggtctgca gatcgctcgc ccaggctgtc actgcggcgt tggtcttctg gtcgcggagc
     1381 tgctgcttga tctgcgcctt gacctctttg taaggagtcg ttcgcgccgg cttcacctcg
     1441 gagagcggct ggatcaagtg gtagccgaac tcggtcttca ccggccgtga cagtgcgtct
     1501 ttcgggagca ggaaggcagt tgtgtcgaac ggcttcaccg tctgccctcg tgtcaccgtg
     1561 agctggccgc ccgcatcttt cgagccgggg tcgagcgagt gctgcttggc gagcgctgca
     1621 aaatctgcgc cggccttcag ctccgagtag aggccgttcg cctgcttctt cgtcttgacg
     1681 aggatatgcc gcacgtcgcg gctctctggg acgtcgaact gggccttgtt ctcgttgtag
     1741 tactgcttgg cgtcggcgtc ggtgaccttg aggcccttcg tcacctcttc gaacaacttg
     1801 tccgtcacga gctgcgcatg gatgtcggag cggaagctct cgtccgtgta gccctgctgc
     1861 ttcagctgct tggagaactc cttctcgttg ccgccgaaga actcttcctt cacttcagcc
     1921 acgcgatcgt cgacttcctt gtccgtgacg gcgatcccca gctcctcggc tttttggtcg
     1981 tactgggcgc gctggaccaa gaaggaggtg gcctgcgtct gcagggcttg gtactcactg
     2041 gtgccgacct tggggaagtc ctgctgctgc gcctcgtagg agaccttcgc ccgcgcgagc
     2101 agctcgtcga gctgctcctt ggtgatgacg acgccgtcga ccgaggcaac gccgccctcg
     2161 ggcacggatt cgccgctgct gccgccgcac ccggctgcga ccaggactgc gagcaggccc
     2221 agtacggaga cagacaggag taggcgccgc gtcatgctgc tctcacttat agacgagggt
     2281 gtgttgcgcc gaggttgagt ttcatgtgca tacggtacgg atcgccgatc gtgtcgctgt
     2341 gagcccgtaa aaagggccgc ccggtcaggc ggcgagacgc gcatcgacta tagcatcggc
     2401 cagctcgagc gctccgcgca gatcatctga gcgccgtgac acttcgcgct tggcggtgct
     2461 gtacacggcc gtttcggaga tcgcgcggag ggcgcggatc tcgtgcgagg cgagcggaat
     2521 cggaccgacg gtcgcacgcc cgccgcggag aacgaggtag tcggctccga gacgcgcgag
     2581 cttcagcttc gcctcctgga tcgcgaacag gttgcccacg ggctcgggca gcggcccgta
     2641 gcggtcttcg gtcgctgcct cgagctcccg aagctcgtcg tccgactcga ccagcgcgag
     2701 gcgacgatgc aggtcgatct tgagcgcctc cgactcgatg tagctggccg ggacgaacgc
     2761 gtccacgcga gcatcgaccc ggacgggcct ggcaacgacg cgggcttggc ccgtgagctc
     2821 cgccacagcc tcgccgagca tctcgacgta gagctcgaag ccgagcgccg cgacgtggcc
     2881 cgactgctcg gcgccgagga gatcgccggc gccccgaatc tcgagatcac gcatcgcgat
     2941 ctggaagccg gcaccgagct cggtgtgatc tgccagggtg gccagcctcg ctctcgcctc
     3001 cggcgtcagc tcggacgcgt cgggatagaa gaggtacgcg tgtgccgtca cgtccgagcg
     3061 gccgacgcga ccacggatct ggtacagctg gctcaggccg agctgatcgg cccgctcgac
     3121 gatcagcgtg ttcgcctggg ggatgtcgat gcccgactcg atgatcgtcg tcgagacgag
     3181 cacgtccccg tcgccggcga ggaaggaatg catccgctct tccaggttgc gctcggccat
     3241 ctgcccgtgc gcgacgagga agcgcagctc cggacacagc tgctgcagct tggccgccgc
     3301 ctcctcgatc gtctccacgc ggttgtggag atagaacgac tggccgcctc tgccgtgctc
     3361 acgcatcagc gcagcggtga tcagctcctc gtcgtactct ccaaccgtcg tacggatcgc
     3421 gcgccttccc tccggcggcg tctcgatgat cgagatgtcg cgcaggcccg acagtgacat
     3481 gtgcagggtg cgagggatgg gcgtggccga gagcgcgaga acgtcgacct cgagccgcag
     3541 cgagcgcaac agctccttct gcgcaacgcc gaagcgctgc tcctcgtcga ggatgaccag
     3601 gccgaggttc ttcgggatca catcgcgcga gagcacgcga tgcgttccga tgagcacgtc
     3661 gaccttgccg gacgagaact cggcgagcac gctcttgacc tccgcggtgc ggcggaagcg
     3721 cgacaccatc tcgaccgtca cgggaaggtc gcgaaagcgc tcgcgaaacg tgttccagtg
     3781 ttgctcggcc agaatcgtcg tcgggcacag aaacagcgtc tgcctgctgt tcgccgcaac
     3841 cgcgaaggcg gccctgacgg caacctccgt cttgccgaag ccgacgtcgc cgcaaacgag
     3901 tcggtccatc gggcgggccg cttcgaggtc ctccttgacg atctcgatcg cccgctcctg
     3961 atcctgcgtc tcgcggtacg ggaacgacgc ctcgagctgt tcgagtaagt cgttctcgag
     4021 gtcgtacgcg acgcccggcg cccgctgccg ctgcgcgtac agcgagagca gctcgcccgc
     4081 gagctcccgc accgaggcgc gggcccgtgc cttgaggttc tgccaggctt tgcccccgag
     4141 cttcgagagc gcgggagccg agccgtcggc gccgacgtac ttggagagct tgccgagctg
     4201 ctcgtgcggg agatagaggc ggtcgtctcc cttgaacgcg accaggaggt agtcacgcgt
     4261 taccgctgcg acctcccgcg tctcgaagcc gagcagcttg ccgacgccgt gatcctcgtg
     4321 gacgacgaag tcacccgagc ggagatctgc gaacgaggcg agcgaccgcc ccaggcgcgg
     4381 atccgcgcgc ggcggacgct tgcggaagac ctgcgtttcg ggcatcagga cgagcccgag
     4441 ctcgcgccag acgaagccgc gccgagcggg ggcgacggcg aagcggaggc tcggctcgcg
     4501 cgggagaggc tcgcccggct cgagcagcgt tccctcgacc ttccgcaaca tcg
//
LOCUS       DBNGNK010000364        10153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_852088,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000364 DBNGNK010000000
VERSION     DBNGNK010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10153
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_852088"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC150V_10795"
     CDS             complement(<1..452)
                     /locus_tag="AC150V_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017844556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR3763445.1"
                     /translation="MAKEILCAYGIDVDAVAGWLGSYGGEDSPDDISRGLFAGEVGTP
                     RLLDLFKRHDLTTTWFIPGHSIETVPRETEMVVEAGHEIGIHGYSHENPIAMSREQEE
                     TILLKCIDLVKDVSGKRPTGYVAPWWEFSPVTNELLLANGIKYDHSLM"
     gene            543..800
                     /locus_tag="AC150V_10800"
     CDS             543..800
                     /locus_tag="AC150V_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763446.1"
                     /translation="MLGNEHWAELEGGRRSRVFRVALLNRVLGHYGLSLGDWSGRVYV
                     LRDRKGRSTVVNDLGSLWVEAERLNGAPLDPLEPALLATLG"
     gene            793..1734
                     /locus_tag="AC150V_10805"
     CDS             793..1734
                     /locus_tag="AC150V_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CobW family GTP-binding protein"
                     /protein_id="HIR3763447.1"
                     /translation="MADAGRIPVSLVTGFLGSGKTTLISRLLTHPELADTAVIVNELG
                     EVAIDHHLIRRVDERTVLLGSGCVCCTLRGDLVDELRDLLDRRDRGEIPPFRRIVVET
                     TGVADPAPIVGTLVSEPFVRSHYELDGLVATVDAVNGLRSDESVRQVAAADSLVVTKV
                     DIADAGPLVTRLRRLNPTAEVIEASFGEVAPARLFGRPHRDPREVTTDGHGHENGVAA
                     IALFLDVQLDWTAFGIWLTMLLQARGSDVLRVKGLLNVGGDGPLLLNGVQHVVHPPEH
                     LDAWPDDDRRSRLVFIGRDLDAAVLEASLQAFDGASR"
     gene            complement(1902..2372)
                     /locus_tag="AC150V_10810"
     CDS             complement(1902..2372)
                     /locus_tag="AC150V_10810"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR3763448.1"
                     /translation="MRAPERILSDAGVAFVVHDHIPVATVAEILEALPFPAEQHVKTL
                     ALDAGGVLALASLRGSDRIRFGPLARALGTARDRLAPLPPERVRAELNLEPGGVCPLV
                     DREDVTCVVDRRVLDLERAFCGSGRADATLELDPSDLVAVSGAAVVDIAALPEA"
     gene            complement(2384..3547)
                     /locus_tag="AC150V_10815"
     CDS             complement(2384..3547)
                     /locus_tag="AC150V_10815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3763449.1"
                     /translation="MDFHLPEELDEIRVAVRELCAGFPGEYWRSLEPDTYPDEFVNAL
                     TENGWLAALVPEEYGGAGLSLTTASVILEEINASGGNAGACHAQMYTMGTILRHGSEE
                     QKQRYLPKIASGELRLQAFGVTEPTAGSDTTQIQTTATPADGGWLVNGQKIWTSRALQ
                     SDLMLLLARTTPADQVEKRTLGLSTFLVDMKRAQADGRMAIRPIKTLMNHATTEIFFD
                     DVFVDAEDLVGEEGMGFRYILDGMNAERVLIAAECIGDGRWFIEKATGYASERVVFGR
                     PIGANQGVQFPIAEAHAQIEAADLMRYKAAWLFEQGLPCGAEANMAKLLAAEASWQAA
                     NACLDTHGGFGFAEEYDVERKFRETRLYKVAPVSNNLILAYLGQHVLGMPRSY"
     gene            complement(3550..4731)
                     /locus_tag="AC150V_10820"
     CDS             complement(3550..4731)
                     /locus_tag="AC150V_10820"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3763450.1"
                     /translation="MSVVELTAAQAEIVAAVREFVDKDVVPVASDLEHRDEYPEELVR
                     TMVEMGLFGTTIPEEYGGLGLGLDTYAMIVAELSRGWVTLSGILNGSFMAATMIRLHG
                     TEAQRETYLPKLASGELRASFSMTEPHAGSDVQSIRTTAERDGDDYVVNGQKMWVTNG
                     WRSGIIMLLAKTDPSADPPHRGMTGFIIEKEPETNPPGLTIPMPGLKKLGYKGVESTE
                     LVFDGFRTPAASVLGGEEGVGLGFKYFMSGIEVGRVNVAARGVGVAQAALDDAIRYAQ
                     EREAFGKPIAHHQAVQLMIAKMATRAEAARLLTLQAAQRKASGERADLEAGMAKYFAT
                     EAAQDNALDCMRIHGGYGYSLEYRPERYYRDAPLLLIGEGSNEIQQLIIARRLLERHA
                     V"
     gene            complement(4728..6125)
                     /locus_tag="AC150V_10825"
     CDS             complement(4728..6125)
                     /locus_tag="AC150V_10825"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR3763451.1"
                     /translation="MATAAETMAGWAEHLSLDDVPKEVIEHAKLHVLDVLGCGLAAHA
                     TGVAGEGRALMAEQGGEAEATVIGLQDGLPAANAAFANAMLCHGLDFDDTHSDSVSHV
                     SAVVTPAALAAAESRSATGAAVLSAIVAGNEIVCRVGMAASGQFHARGFHPTAICGVF
                     GAAAAVAVLDGLDAETTTRALGIAGSFAAGIFAYLADGTPTKPMHPAWAAHGSHIAAG
                     LARLGAIGPHSVFEGKFGLYHAFLAAEEGEIDLASQLADLGSRWETPRIAYKPYPVCH
                     FMHGSLGAAAEAVAGRTFSADEIGEVVVMVPAAGVPLVLEPAAEKEVPRSEYDGKFSL
                     QYSVASQLVRGHVAVSDFTDEAITDPEVLAVAAKVRYEEKEYDSYPQAFPGATRVTLA
                     SGESFESDLAHQKGAPENPFSADEVRAKFRENAGMALPDAELAALEEAVLTLEQAADV
                     TAALAPLTLREVARR"
     gene            complement(6154..6681)
                     /locus_tag="AC150V_10830"
     CDS             complement(6154..6681)
                     /locus_tag="AC150V_10830"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009086395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR3763452.1"
                     /translation="MSATETAPKAWRGRFYEDFDAGDVFRSRLGRTITETDNIWFTCL
                     TLNTNQMHFNTPFTEGTRFGQPLVNSAFTLALVTGLTVPDTSENAAANLAWTDIKLPK
                     PVYIGDTLWAESEILDKRESKSNPNVGIVSMRCRGINQRNEVVIEFKRTFMVYKRDAP
                     EVADTFPETSEDWTV"
     gene            complement(6678..7553)
                     /locus_tag="AC150V_10835"
     CDS             complement(6678..7553)
                     /locus_tag="AC150V_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR3763453.1"
                     /translation="MTRSECLTYNHITSATVENLRSLLFAPGSDERKLDRALNSDADA
                     LIADLEDAVAPDQKDGARETVRRVLGAAPSSGVARVVRVNGADSTHFAADVSAIQDLP
                     LDAVVLPKASVEAVDMLGPDGPPVLALIESARGLRQSFEIATRPRVAALILGSADLGA
                     ELGLEPRRDGLELLYARSQLVVDSAAAGIRAPIDVVHLDISDDAGLEEEARLARSLGF
                     TGKTCIHPRQLPIVNSVFEPGEEEISWATSVLAAYDDGVREGRGAVSVGGEMIDLAVV
                     ERARRVLSLREGGRS"
     gene            7657..8685
                     /locus_tag="AC150V_10840"
     CDS             7657..8685
                     /locus_tag="AC150V_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR3763454.1"
                     /translation="MKAARFHEFGSSDVMQIEEIDDPAPGPGQVSIAIRASALNHLDV
                     DIREGVSRFPVEPPFTPGIEVAGEIDQVGEGVQDWQPGDRVNPYLMDTCGSCRFCRSG
                     RESLCLTPGFISFSTGGGYAEKLVCNERHLIRIPDNVSFEDAAALQVAFATSWHMLFT
                     RAGLQPGETVMINSVGSGIGSAAVQLAHRAGAFVIGNASSDEKLAKAAELGMDVGINH
                     ATEDVVARVMEITDEAGVDVVYEHVGGELFQKGLDSLGKDGRLVICGGHSGEVVPFDI
                     IPFFRMQRSVIGSFTYTSLEVEKCFDLASRGLVKPLVHTTFPLDQAKEAMEMMERREH
                     FGKIVLIP"
     gene            8713..>10153
                     /locus_tag="AC150V_10845"
     CDS             8713..>10153
                     /locus_tag="AC150V_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3763455.1"
                     /translation="MKRVGVDVGGTFTDLIFVDDESGVIQVHKLPTTPDDPSRGTVAG
                     IQEITAEADTEPAALDQVFHGTTIATNIVIEHNGATVGMITTEGYRDILHIARHKKPL
                     NFSNYQDLPWQRYPIVRRRYRLTVPERMTKDGTALVPLDEDKAREQVRRLKEAGVEAV
                     AVCFLFSFLNAEHEQRVADIVREEFPEAFLSVSSEVLPQYREYERFSTVCLNAFVGPK
                     VATYVGRLQDELTGLGVRTGVHLMTSASGVATPEAAVKRPVNLLMSGPVAGVVGGIWA
                     GKQAGYENVITLDVGGTSADIGLAQEGRLRMKHLLDTKVGPYQAMIPMADVDTIGAGG
                     GSIAYVDQGGIFRVGPRSAGAEPGPACYDKGGTEPTATDAMVNLGWLPAEDFLGGGMT
                     LRTELAEEAFAKGPAAALGMSVEEASMGAVQILTHSMVQSIEENSVRKGFDPRDFALV
                     AEGGAGPLFAAQIALEVGTPNVLVPPYPGV"
BASE COUNT         1600 a         3510 c         3392 g         1651 t
ORIGIN      
        1 tgcatgaggc tgtggtcgta cttgatcccg ttggcgagca gaagctcgtt cgtgacaggt
       61 gagaactccc accacggggc gacgtagccg gtcgggcgct tcccggagac gtccttcacg
      121 aggtcgatgc acttgagcag gatcgtctcc tcctgctctc tcgacatcgc gatcgggttt
      181 tcgtgcgagt acccgtggat gccgatctcg tggcccgcct cgacgaccat ctcggtctcg
      241 cgcgggaccg tctcgatcga gtggccgggg atgaaccagg tcgtcgtcag gtcatggcgc
      301 ttgaacaggt cgagcaagcg cggcgttccc acctcgccgg cgaacagccc gcgtgagatg
      361 tcgtccggcg agtcctcgcc gccgtacgag ccgagccatc cggcaacggc gtccacgtcg
      421 atgccgtacg cgcacaagat ctccttcgcc atcgctgctc actcctcggg gtcgcgtaaa
      481 tgggcttcga acctactacg cgggctgcta caacgccaaa gctgatgtgt tcgctgtgca
      541 atgtgctggg gaacgagcac tgggccgagc tcgagggggg ccgtcgctcg cgcgtgttcc
      601 gcgtggcgct gctcaatcgc gtcctcggtc actacggcct cagcctcggt gactggagcg
      661 ggcgcgtgta cgtgctccgc gaccgcaagg gccgctcgac ggtcgtgaac gacctcggct
      721 cgctctgggt agaggcggag cggctcaacg gcgccccgct cgaccctctc gagcccgcgc
      781 tactggcgac gcttggctga cgccggccgg atccccgtct cgctcgtcac gggcttcctc
      841 ggctcgggca agacgacgct gatctcacgg ctgcttacgc acccggagct cgccgacacc
      901 gccgtcatcg tcaacgagct cggcgaggtc gcgatcgacc atcacctcat tcgccgggtc
      961 gacgagcgga cggtgctgct ggggtccggc tgcgtctgct gcacgctgcg gggcgacctc
     1021 gtcgacgagc tgcgcgacct cctcgaccgg cgcgatcgcg gcgagatacc gccgttccgg
     1081 cggatcgtcg tggagaccac cggtgtcgcc gacccggccc cgatcgtcgg cacactggtg
     1141 tcggagccct tcgttcgcag ccactacgag ctcgacggac tcgtcgcgac ggtcgacgcc
     1201 gtgaacggtc tccgctccga cgagtccgtc cggcaggtcg cggccgctga ctcgctcgta
     1261 gtgacgaagg tcgacattgc cgacgccggg ccgctggtga cgcggctgcg tcggctcaat
     1321 ccgacggcgg aggttatcga ggcgtcgttc ggtgaggtgg cgccggcgcg gctgttcggg
     1381 cgcccgcacc gagacccacg tgaagtgaca accgacgggc acgggcacga gaacggtgtc
     1441 gctgcgatcg cgctcttcct cgacgttcag ctcgactgga ccgcattcgg gatctggctg
     1501 acgatgctgc tccaggcgcg cggctctgac gtccttcgcg tcaagggctt gctgaacgtc
     1561 ggtggcgacg ggccgctcct gctcaacggc gtgcagcacg tcgtgcaccc gccggagcat
     1621 ctcgacgcct ggccggacga cgatcggcgc tcgcgtctcg tcttcatcgg gcgcgatctc
     1681 gacgccgccg tgctggaggc gtcgctgcag gcgttcgacg gcgcgagccg ctagcgggcg
     1741 cccggggccg gcgaagcgcc gcttacgggc gtgtcttctt gcccgccgaa ggacacgtcg
     1801 gacctctgag gtccgctcac cgcgttcgga cctgtcgaac gactgcgcag tgcgcgaaac
     1861 gggttgtcgg gtcctcagag gtgggactgg gccgtaaggg actaggcctc ggggagcgcc
     1921 gcgatgtcga cgacggccgc tcccgagacc gcgaccaagt cgctcgggtc cagctcgagc
     1981 gtggcatcgg cgcgcccgga gccgcagaac gcgcgctcga ggtcgaggac gcggcgatcc
     2041 acgacgcagg tgacatcctc acggtcgacg agaggacaca cgcctcccgg ctcgagattg
     2101 agctctgctc gcacgcgctc cggcggcagc ggcgcaagcc ggtcgcgcgc ggtgccgaga
     2161 gcgcgggcga gcggcccgaa acggatcctg tcgctacccc gcagcgaggc gagcgcgagc
     2221 acgccgcccg cgtcgagcgc gagcgtcttg acgtgctgct cggccgggaa gggcagcgcc
     2281 tccaggatct cggcgacagt cgcgaccgga atgtgatcgt gcacgacgaa cgccactccg
     2341 gcgtccgaga ggatcctctc cggggctcgc acgtccccgc tcgctagtac gagcgaggca
     2401 tgccgaggac gtgctgtccc aggtacgcga ggatgaggtt gttcgagacg ggcgcgacct
     2461 tgtagaggcg cgtctcgcgg aacttgcgct cgacgtcgta ctcctccgcg aagccgaagc
     2521 cgccgtgcgt gtcgaggcag gcgttcgccg cctgccagga cgcctcggca gcgagcagct
     2581 ttgccatatt ggcctccgct ccgcagggaa gcccctgctc gaacagccac gccgccttgt
     2641 accgcatcag atcggcggcc tcgatctgcg cgtgcgcctc ggcgatcggg aactgcacgc
     2701 cctggttcgc gccgatcggc cgcccgaaga cgacgcgctc cgacgcgtag ccggtcgcct
     2761 tctcgatgaa ccagcgcccg tcgccgatgc actcggcggc gatgagcacc cgctcggcgt
     2821 tcatgccgtc gaggatgtac cggaagccca tcccctcctc gccaacgagg tcctccgcgt
     2881 ccacgaacac gtcgtcgaag aagatctcgg tcgtcgcgtg gttcatcagc gtcttgatcg
     2941 ggcggatcgc catcctcccg tccgcctggg cgcgcttcat gtcgacgagg aacgtcgaca
     3001 gaccgagcgt ccgcttctcg acctgatccg ctggggtcgt gcgcgcgagg agcagcatca
     3061 gatcggactg gagcgcgcgc gaagtccaga tcttctgacc gttcacgagc cagccgccgt
     3121 ccgccggcgt cgccgtcgtc tggatctgcg tcgtgtccga gcctgcggtc ggctccgtca
     3181 cgccaaaggc ctggagcctg agctcgccgg aggcgatctt cggcaggtag cgctgcttct
     3241 gctcctccga gccgtgccgc aggatcgttc ccatcgtgta catctgagcg tggcaggcac
     3301 cggcgttgcc ccccgacgcg ttgatctctt ccaggatgac gctcgccgtc gtcagcgata
     3361 ggcccgctcc gccgtactcc tcggggacga gggcggccag ccacccgttc tcggtcagcg
     3421 cattgacgaa ctcgtccgga taggtgtccg gctcgagtga gcgccagtac tcccccggga
     3481 agccggcgca cagctcccgc accgcgacac ggatctcatc cagctcctct gggaggtgaa
     3541 agtccatgct cagaccgcgt gccgctcgag gagccgccgg gcgatgatca gctgctggat
     3601 ctcgttcgag ccctcgccga tcagcagcag cggcgcgtcg cggtagtagc gctcgggccg
     3661 gtattcgagc gagtagccgt aaccaccgtg gatccgcatg cagtcgagcg cgttgtcctg
     3721 tgcggcctcg gtggcgaagt acttcgccat cccggcctcc aggtcggccc gctcgcctga
     3781 tgccttccgt tgggccgcct gcagcgtcag gagccgcgcc gcctccgcac gcgtcgccat
     3841 cttggcgatc atcagctgga ccgcctggtg atgggcgatg ggcttgccga acgcctcgcg
     3901 ctcctgcgcg tagcggatcg cgtcgtcgag agccgcctgc gccactccga caccacgcgc
     3961 tgcgacgttc acccgtccga cctcgatccc gctcatgaag tacttgaagc ccagtccgac
     4021 gccctcctcg cccccgagca cgctcgcggc cggcgtccgg aagccgtcga agaccagctc
     4081 ggtcgactca acgcccttgt agccgagctt cttcagcccc ggcatcggga tggtgaggcc
     4141 gggcgggtta gtctcgggct ccttctcgat gatgaagccc gtcatcccac ggtgcggcgg
     4201 atcggcggac ggatcggtct tggcgagcag catgatgatc cccgagcgcc agccgttcgt
     4261 gacccacatc ttctgtccgt tgacgacgta gtcgtcaccg tcccgctcgg ccgtggtgcg
     4321 aatcgactgg acgtccgagc cggcgtgggg ctccgtcatc gagaaggagg cacgaagctc
     4381 gcccgaggcg agcttgggca ggtaggtctc ccgctgcgcc tcggtcccgt gcaggcggat
     4441 catcgtcgcg gccatgaacg agccgttgag gatgccggag agcgtgaccc agccacgcga
     4501 gagctcggcg acgatcatcg cgtacgtgtc gaggccgagc ccgagaccgc cgtactcctc
     4561 cgggatcgtc gtcccgaaga ggcccatctc gaccatcgtc cggacgagct cctccggata
     4621 ctcgtcgcgg tgctcgaggt cggacgccac ggggacgaca tccttgtcga cgaactcccg
     4681 taccgcggcg acgatctccg cctgcgcggc ggtcagctcg acgacgctca tctgcgcgca
     4741 acctcccgga gcgtgagcgg cgcgagcgcg gcggtcacgt cggcggcctg ctcgagcgtg
     4801 agcacggcct cctcgagcgc cgccagctct gcgtcgggca gcgccatccc cgcgttctcg
     4861 cggaacttcg cccgcacctc gtccgcgctg aacgggttct ccggcgcacc cttctggtgt
     4921 gcgaggtcac tctcgaacga ctcgccgctc gcgagcgtca cccgcgtagc ccccgggaac
     4981 gcctgcggat agctgtcgta ctctttctcc tcgtaccgca ccttcgcggc cacggcgaga
     5041 acctcggggt cggtgatcgc ctcgtcggtg aagtcggaga cggcgacgtg gccacgcacg
     5101 agctgcgagg cgaccgagta ctgcagcgag aacttgccgt cgtactcgga gcgcggcacc
     5161 tccttctcgg cggccggttc caggacgagc ggaactcctg cggccggaac catgacgacg
     5221 acctcgccga tctcgtcggc cgagaacgtg cgtcccgcta cggcctcggc agccgccccg
     5281 agcgacccgt gcatgaagtg gcagaccgga tagggcttgt aggcgatgcg cggcgtctcc
     5341 cagcggctgc cgagatcggc aagctgcgag gccagatcga tctcgccttc ttccgccgcg
     5401 aggaacgcgt ggtagaggcc gaacttcccc tcgaacacgg agtgcgggcc gatcgctccg
     5461 agcctcgcca ggccggccgc gatatgcgac ccgtgggcgg cccacgcggg atgcatcggc
     5521 ttcgtcggcg tcccgtcggc gaggtaggcg aagatcccgg ccgcgaacga cccggcgatc
     5581 ccgagcgccc tggtcgtggt ttcggcgtcg agcccgtcga gcacggccac cgccgccgcc
     5641 gcgccgaaca cgccgcagat ggctgtcgga tggaagccgc gggcgtggaa ctgccccgag
     5701 gcggccatac cgacgcgaca gacgatctcg ttgccggcga cgatcgccga gagcaccgcg
     5761 gcgcccgtcg cagaacggga ctcggcggcg gcgagagcag ccggggtcac gaccgccgag
     5821 acatggctca ccgagtcgga atgggtgtcg tcgaagtcga gaccgtggca cagcatcgcg
     5881 ttcgcgaaag cagcattcgc ggccggcaaa ccgtcctgca ggccgatcac cgtggcctcc
     5941 gcctctcccc cctgctccgc catcagtgcc ctgccctcgc cggcgacccc cgtcgcatgc
     6001 gcggcaagcc cgcagccgag cacgtcgagg acgtggagct tggcgtgctc gattacctct
     6061 ttcgggacgt cgtcgagcga gaggtgctct gcccaacccg ccatcgtctc tgctgctgtc
     6121 gccatctctt cgtctccttc cggcccgtcc gcctcagacg gtccagtcct cgcttgtctc
     6181 cgggaatgtg tcggccacct cgggcgcatc gcgcttgtac accatgaacg tccgcttgaa
     6241 ctcgatcacg acctcgttcc gctgattgat gcctcgacac cgcatcgaga cgatgccgac
     6301 gttgggattg gacttcgact cacgcttgtc gaggatctcg ctctcggccc agagcgtgtc
     6361 accgatgtag accggcttgg gcagcttgat gtcggtccac gcgaggttcg cggccgcgtt
     6421 ctcgctcgtg tcgggaaccg tgagccccgt gacgagcgcc agagtgaaag cgctgttgac
     6481 gagtggctgg ccgaacctcg tgccctcggt gaacggcgtg ttgaagtgca tctggttcgt
     6541 gttcagcgtt aggcaggtga accagatgtt gtcggtctcg gtgatcgtcc gcccgagccg
     6601 cgagcgaaac acgtcacccg catcgaagtc ttcgtagaaa cgtcctcgcc acgcctttgg
     6661 agccgtctcg gtcgcactca tgaacgtcct ccttcccgta gtgaaagcac ccgccgcgcg
     6721 cgctcgacga ccgccagatc gatcatctcg cctccgaccg acacggcgcc ccggccctcg
     6781 cgcacgccgt cgtcgtaggc ggccagtacc gaggtcgccc aactgatctc ctcctcgccc
     6841 ggctcgaaga ccgagttgac gatcgggagc tggcgcgggt ggatgcaggt cttccccgtg
     6901 aagccgagcg agcgcgcaag gcgcgcttcc tcctcgaggc cggcgtcgtc cgagatgtcg
     6961 aggtggacga cgtcgatcgg ggcgcgaatt ccggccgccg cgctgtcgac gacgagctgc
     7021 gaacgggcgt agagaagctc gagaccatcg cggcgcggct cgagtccaag ctcggcgccg
     7081 aggtcggcag agccgaggat cagggcggcg acgcgcggac gagtcgcgat ctcgaacgac
     7141 tgccgcagcc cgcgcgccga ctcgatgagc gccagcacag gcgggccgtc cggcccgagc
     7201 atgtcgacgg cctcgaccga cgccttgggc aggacgaccg cgtcgagggg caggtcctgg
     7261 atcgcgctga cgtcagccgc gaagtgcgtc gaatcggctc cgttcacgcg aaccactcgc
     7321 gccacgccgg agctcggcgc cgcgccgagc acgcgtcgca ccgtctctcg cgcaccgtcc
     7381 ttctggtccg gcgcgacggc gtcctccaga tcagcgatga gcgcgtcggc gtccgaattc
     7441 agcgcacggt cgagctttcg ctcgtctgaa ccgggagcga agagaagcga gcggaggttt
     7501 tcgaccgtcg cggacgtgat gtggttatat gtcagacatt cggatcgtgt caaacggctt
     7561 caccgtgccc cgtggcgcgc accctgcttg acacgccttc gaaagtcaga cataaaaaca
     7621 ccccagtgaa cgcatgggag gcgggaggtc gatgccgtga aggctgcgcg attccacgag
     7681 ttcggcagct ctgacgtgat gcagatcgag gagatcgacg atccagcgcc cgggccgggc
     7741 caggtctcga ttgcgatccg agcgagcgca ctgaaccacc tggacgtcga catcagggag
     7801 ggcgtctccc gcttccccgt cgagcctccg ttcaccccgg ggatcgaagt cgccggcgag
     7861 atcgaccagg tcggcgaggg cgtacaggat tggcaacccg gcgatcgggt caacccgtac
     7921 ctcatggaca cctgcggcag ctgtcgattc tgccgctccg gacgcgagtc actctgtctg
     7981 acgccaggct tcatcagctt ctcgaccggc ggcggctacg cggagaagct cgtctgcaac
     8041 gagcgccacc tcatacggat cccggacaac gtctcgttcg aggacgcagc cgcgctgcag
     8101 gtcgccttcg caacctcgtg gcacatgctc ttcacgcgcg ccgggctcca gcccggcgag
     8161 acggtgatga tcaactccgt cggttctggc atcggatcgg cagccgtcca actcgcccac
     8221 cgcgcgggcg cattcgtgat cggcaacgcg tcgtcggacg agaagctcgc gaaagccgcg
     8281 gagctcggca tggacgtggg catcaaccac gcgaccgagg atgtcgtcgc ccgcgtgatg
     8341 gagatcaccg acgaggccgg cgtggacgtc gtctacgagc acgtcggcgg cgaactcttc
     8401 cagaaggggc tcgactcgct cgggaaggac ggcaggctcg tgatctgcgg gggccactcc
     8461 ggcgaagtgg tgcccttcga catcatcccg ttcttccgca tgcaacgcag cgtgatcggc
     8521 tcgttcacat acacgagcct cgaagtcgag aagtgcttcg atctcgcgtc gcgcggactc
     8581 gtcaagccac tcgtccacac gaccttcccg ctcgaccagg cgaaagaggc gatggagatg
     8641 atggagcgcc gcgagcactt cggcaagatc gttctcatcc cctagcccag tgaacctcaa
     8701 ggagagaagc gtatgaagcg tgttggggtc gacgtcggcg gcacgttcac cgacctgatc
     8761 ttcgtcgacg acgaatcggg cgtgatccag gtgcacaagc tcccgacgac gcccgacgat
     8821 ccctcgcgag gaaccgtggc ggggatccag gagatcactg cggaggcgga caccgagccg
     8881 gccgcactcg atcaggtgtt ccatggaacg acgatcgcga cgaacatcgt catcgagcac
     8941 aacggcgcga ccgtcgggat gatcaccact gagggctacc gcgacatcct gcacatcgcg
     9001 cggcacaaga agcccctgaa cttctcgaac taccaggatc tgccgtggca gcgctacccg
     9061 atcgtcaggc gccgctaccg cctcaccgtg ccggagcgca tgacgaagga cggcaccgcg
     9121 ctcgtgccgc tcgacgagga caaggcgcgt gaacaggttc ggcgcttgaa agaagccggc
     9181 gtcgaggcgg tggcagtctg cttcctgttc tcgttcctca acgccgagca cgagcagcgc
     9241 gtggccgaca tcgtgcggga ggagttcccg gaggctttcc tttccgtctc ctcggaggtc
     9301 ctgcctcagt accgcgaata cgagcgcttc tccaccgtct gtctcaacgc gttcgtcggc
     9361 ccgaaggtcg cgacgtacgt ggggcggttg caggacgagc tgaccggcct cggcgtgcgg
     9421 acgggcgtcc atctgatgac gtccgcaagc ggcgtggcga cgccggaggc cgccgtcaag
     9481 cgccccgtca acctgctcat gtccggcccg gtcgccggcg tggtcggcgg gatctgggcc
     9541 ggcaagcagg ccggctacga aaacgtcatc accctcgacg tcggcggcac gtcggccgac
     9601 atcggactgg cccaggaagg tcggctgcgg atgaagcacc tcctcgacac gaaggtaggt
     9661 ccgtatcagg cgatgatccc catggccgac gtcgacacga tcggcgccgg aggaggctcg
     9721 atcgcatacg tcgaccaggg cgggatcttc cgtgtcggtc cgcgctcggc cggggccgag
     9781 cccggaccgg cgtgctacga caagggtggc accgagccga cggcgaccga tgccatggtc
     9841 aacctcggct ggctgccggc cgaggacttc ctcggaggcg gcatgacgct caggacagag
     9901 ttggccgaag aggccttcgc gaaggggccc gctgccgcac tcggcatgtc ggtcgaggag
     9961 gcgtcgatgg gcgccgtcca gatcctgacg cactcgatgg tgcagtcgat cgaggagaac
    10021 tcggtgcgca agggcttcga cccacgcgac ttcgcgctcg tcgccgaagg cggcgcgggg
    10081 ccgttgttcg cggcccagat cgccctcgag gtcgggacgc ccaacgtgct cgtgccgccg
    10141 tacccgggcg tca
//
LOCUS       DBNGNK010000365         5386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_564273,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000365 DBNGNK010000000
VERSION     DBNGNK010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_564273"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..316)
                     /locus_tag="AC150V_10850"
     CDS             complement(38..316)
                     /locus_tag="AC150V_10850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020243.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR3763456.1"
                     /translation="MPSALVTGGSSGIGLAIARMLASEGYSLTLAARRVEPLEAAANE
                     LGAESVRADVTIEEDCARLVETHAGVFDGLDVLVNSAGVGIARPLADM"
     gene            471..1496
                     /gene="selD"
                     /locus_tag="AC150V_10855"
     CDS             471..1496
                     /gene="selD"
                     /locus_tag="AC150V_10855"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR3763457.1"
                     /translation="MSRTSQLTSLAQAGGCAAKYPAARLEQLLAGFAPAEAEDLLVGL
                     DPADDAAVYRLDDERALVFTIDFFPPVVDDPREFGAIAATNALNDVYAMGGMPLLALS
                     VAAFPEDLPPSLLGDMLAGAHDAVRAAGAILAGGHTIRDAEPKYGLAVVGTVHPAGVW
                     VKSGARPGDDLFLTKPLGTGLVLQARRESRDPPGALAAAIESMLELNRAAADALRTFS
                     PSAVTDVTGFGLAGHAYELADRSGVRAVIEADALPALPHALELAKAGIRTGGERRNRD
                     YAEPHMESSAPPELDALAYDPQTAGGLLIAVPADKSAVLQASFVGQGLQLWRIGRVES
                     GTGVVLA"
     gene            1564..2535
                     /locus_tag="AC150V_10860"
     CDS             1564..2535
                     /locus_tag="AC150V_10860"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR3763458.1"
                     /translation="MSRGGAKRRVSDRRFVQLSIASLLGLFVVVTSGAFVRLTDSGLG
                     CDNWPRCGDKPFPEKGFHAFVEFGNRLVAVVGIVFALAVWLAARRVEGLPRGVRLTAL
                     AAFLGTVAQIPLGGVTVILELHPLAVMSHFLLALVVVALAVIIALEAWSHARGHGAAI
                     GPNWWRLLASVGVVACLALVVTGAVATASGPHSGGEDIRRLGITVTDAVWVHVRATAV
                     YGIGLVVVGLWIVRHRARVPGLVWLGGLLLIVLLAQMLVGEIQYRNALPWGIVLVHVF
                     LGAAIWALTVAFVHSLWRPPLPLLSGAARKRTPAAPGRAQSGSVAVD"
     gene            2592..3491
                     /locus_tag="AC150V_10865"
     CDS             2592..3491
                     /locus_tag="AC150V_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HIR3763459.1"
                     /translation="MDTSELRLLERPTLERPVLIAAFRGWNDGGQGATLAAGYLSRHW
                     KASRFADIDPEGFIDFQTTRPQVKLDEGSTRKVVWPENTFHHARIPGLERDAVILTGV
                     EPNYRWRHFSDLVLSLARDLSVELVVTFGALLADVPHSRPAPVTGAATDLKLVEELGL
                     ELSRYEGPTGIVGVMLDACRRAELPAVSLWSAVPHYVSLAPSPRAARALCERLSALLD
                     VSIDLEELEQAEAKYADQVSEAVAADADTAAYVEELERRTDTLGELVDESTLPSGDSI
                     AAELTRFLRDREGRDEGPQKNKE"
     gene            complement(3493..>5386)
                     /locus_tag="AC150V_10870"
     CDS             complement(3493..>5386)
                     /locus_tag="AC150V_10870"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR3763460.1"
                     /translation="GAIEAGATATTANPITVQTYAWFARHGKSLSRTAYHLVICDEAH
                     TALGEKTSAAIRALPEPLYIGMTATEQLIAKQVSDVFPASVDDLPLADAARRGLIAPL
                     RCLRVPPAAAINSVPIVGGDFEERALADALDHQALNQAAASLYRDRFASTPGIVYAAG
                     VDHAYNLAKEFRAAGLQAEAVSGRTPPVRLAETLAAYERGEINVLINAQLLAEGWNSP
                     RATVCMHLAPTASRRVYQQRIGRIMRIHPRKEAGVVIDFVPKSATHSERVVSLHSLLD
                     SDFYREGARVTPAPRRRSQRRARRRLSPAPWLVPVTPDVARRLNVIQREWQRIDPKYL
                     DEDEQRFWATIAGRQIRFDQRAEFARKFAEGRAGKAALETFLATCAAENSNRRLRMTA
                     LADRVSTPVERADFDDLVTLVTQAPPWEKDRAAGVRILLRAIGDGKASAPEQILARWT
                     WRLARATRKTQDRRASQEFPEAKRLLGAVANSRGHRHEENVARLAKVAVALPLQIGAA
                     LLASAEGYTPRANGTLDRAREELGSIDAIAYALAENLPPPKAPGRKSRRRRRKRKAKA
                     QAARNENSGRQGASGPVEGDGRPGVDETAAGDGAAAKAEQRSAAGGAGGEPAAERGST
                     DGRPTT"
BASE COUNT          761 a         1859 c         1869 g          897 t
ORIGIN      
        1 ggagattcac ggcctgctgc aaatcccagt gcttcgtcta catgtcggcg aggggccggg
       61 cgatcccgac cccggcggag ttgacgagga catcgagtcc gtcgaagacg ccggcgtgcg
      121 tctcgaccag gcgcgcgcag tcctcctcga tggtgacgtc ggccctgacg ctctcggcgc
      181 cgagctcgtt cgctgccgcc tccagtggct cgacccgtcg tgccgcaagc gtgagcgagt
      241 agccctccga ggccagcatc cgcgctatcg caagcccgat tccggacgag ccgccggtga
      301 cgagggcgct cggcacggac acgattttct cacagacggc gcgacggccg ccgagggggt
      361 gacggccgtc gcgtcgtcag gggagaggag atagggggat gagtctctcc agcgcctgtg
      421 gttcgccgcg tcccgccttc ctccttctcg cctgcgtaca ctcatcgcac atgagtcgga
      481 cgtcacaact gacttcgctc gcccaagcgg gaggctgcgc cgccaaatac cctgccgcgc
      541 ggctggaaca gctgctcgcg ggtttcgcgc cggccgaggc ggaggatctg ctcgtcggcc
      601 tcgatcccgc cgacgacgcg gcggtgtacc gcctcgacga cgagcgggca ctcgtcttca
      661 ccatcgactt ctttccaccg gtggtggacg acccgcggga gttcggtgcg attgccgcca
      721 cgaacgccct caacgacgtc tacgcgatgg gtgggatgcc gctgctcgcg ctttcggtgg
      781 cggccttccc cgaggatctt ccaccgtcac tgctcggcga catgctcgcg ggagcgcacg
      841 acgccgtccg ggccgccggt gcgattcttg ctggcggcca cacgatcagg gacgctgaac
      901 cgaagtacgg cctcgccgtg gtcgggacgg tgcatccggc aggcgtctgg gtcaaaagtg
      961 gcgcgcggcc gggcgacgac ctcttcctga cgaagccgct cggaacgggg ctcgtgctgc
     1021 aggcacgccg cgagagtcgc gatccaccgg gcgccctcgc agcggcaatc gagtcgatgc
     1081 tggaactcaa ccgcgccgct gcggacgcgc tccgaacttt ttcgccatca gcggtcaccg
     1141 acgtcaccgg cttcggcttg gcaggccacg cgtacgagct cgccgaccgc agcggcgtcc
     1201 gcgcggtcat cgaggccgac gcactgcccg ctctccccca tgcgctggag ctcgcgaagg
     1261 ccggcattcg caccggcggg gagcgccgca accgagacta cgccgagccg cacatggaaa
     1321 gctccgcgcc accagagctc gacgctctcg cgtacgaccc acagactgcg ggggggctgc
     1381 tgatcgccgt acccgccgac aagagcgccg tgctgcaggc gtccttcgtc ggccaggggc
     1441 ttcagctctg gcggatcggg cgggttgaga gcgggacggg tgtcgtactc gcctagcgcc
     1501 ccgccatcgg gagctgcgcg aggacccgca agcggattcg cgacagggaa ctaaactcga
     1561 aacgtgagtc gcgggggcgc caaacgccgg gtctctgacc gccgttttgt ccagctctcc
     1621 atcgcgtcgc tcctgggtct cttcgtcgtt gtcacctcgg gtgcattcgt gcggctcacc
     1681 gactcgggac tcggctgcga caactggccg cgctgtggcg acaagccctt tccggagaag
     1741 ggattccacg cgttcgtcga gttcgggaac cgcctggtgg ccgtggtcgg gatcgtcttc
     1801 gcgctcgctg tctggctcgc tgcacgccgt gtggaggggc tgccacgggg tgttcgcctg
     1861 acggcgctcg ccgcctttct gggaacggtc gcccagatcc cgctcggcgg cgtaacggtc
     1921 attctcgagc ttcatccgtt ggcggtgatg tcgcacttcc tgctcgcgct ggtcgtcgtg
     1981 gccctggccg tgatcatcgc gctcgaggcc tggagtcacg cgcgcggcca tggtgcggcg
     2041 atcggaccga actggtggcg actgctcgca tcggtaggcg tggtcgcctg cctggccctt
     2101 gtcgtgaccg gcgcagtcgc aaccgcatcg ggaccacact ccggcggcga ggacatccgc
     2161 aggctcggga tcaccgtcac ggacgccgtc tgggtgcacg tgcgggcaac agccgtctac
     2221 ggcatcgggc tcgtcgtcgt cggactctgg atcgtgcgcc accgggcgcg ggtgccgggc
     2281 ctcgtctggc tcggcgggct gctgttgatc gttctgctcg cccagatgct cgtcggggag
     2341 atccagtacc gaaatgcact tccctggggg atcgtgctcg ttcacgtctt tctcggagcg
     2401 gcaatctggg cgctcacggt cgccttcgtt cattcgctct ggcgccctcc gctgccgctt
     2461 ctcagcggcg cggcccggaa acgcacgccc gcggcgcccg ggcgcgccca aagcggctcg
     2521 gtggccgtcg actagccggg atcgggaagc cggccggggc tggccagcgg gtgggcgact
     2581 aggcttcaac cgtggacacc agcgagcttc gccttctcga acgacccacg ctcgagcgac
     2641 cagttctgat cgcggcgttc cgcggctgga acgacggagg ccagggagcg acgcttgcag
     2701 ccggctacct gtcgcgacac tggaaggcct ctcgcttcgc cgacatcgac ccagagggct
     2761 tcatcgactt ccagacgact cggccacagg tcaagctcga cgagggatcg acgagaaagg
     2821 tcgtgtggcc cgagaacacg tttcaccacg cgcggattcc ggggcttgaa cgcgacgcgg
     2881 tcatcctcac cggcgtcgag cccaattacc gctggcgcca tttcagtgac ctggttctct
     2941 cgctcgcgcg tgatctgagc gtcgaactcg tcgtcacgtt cggcgcgctg ctcgcggacg
     3001 tcccgcacag ccgccccgct cccgtgaccg gtgccgcgac cgacctgaag ctcgtcgagg
     3061 agctcggact cgagctctcg cgctacgagg ggccgacggg catcgtcggc gtgatgctcg
     3121 acgcatgccg ccgggccgag ctacccgcgg tgagcttgtg gtcggcggtc ccgcactacg
     3181 tctcgctcgc gccgagcccg cgggctgccc gcgccctctg tgagcgtctg agcgcgctac
     3241 ttgacgtctc gatcgacctc gaggagctcg agcaggccga ggcgaagtac gccgaccagg
     3301 tcagcgaggc ggtcgcggca gacgccgata cggctgccta cgtcgaggag ctggagcgtc
     3361 gaaccgacac tctgggtgag ctggtcgacg agagcacgct cccgtcgggc gactcgatcg
     3421 cagccgagct gacccgcttc ctccgcgacc gcgaggggcg ggacgaaggt ccgcagaaaa
     3481 acaaggagta ggtcaggtcg tcggccgacc gtcggtcgag ccccgctcgg cggcgggctc
     3541 accgcccgcc ccgcctgcgg ctgaccgttg ttccgccttc gccgcggccc cgtctcctgc
     3601 ggcggtttca tcgactcccg gacgaccatc gccttcgacg ggcccggagg cgccctggcg
     3661 tccggagttc tcgttgcgtg ccgcctgcgc cttcgccttt cgctttcggc gacggcgccg
     3721 cgacttgcgc cccggcgcct tgggtggcgg caggttctct gccagcgcgt acgcgatggc
     3781 gtcgatcgag ccgagttcct cccgcgcgcg gtcgagcgtt ccgtttgcgc gcggcgtgta
     3841 gccctcggcc gaggcgagga gggccgcgcc gatctgcaac ggcagcgcca cggcaacctt
     3901 ggccagtctg gcgacgttct cctcgtggcg gtggccgcgc gagttcgcca ctgctccgag
     3961 caggcgcttc gcctcgggaa actcttgact ggcgcgacga tcctgcgtct ttcgggtcgc
     4021 gcgcgcgagc cgccatgtcc agcgcgcgag tatctgttcg ggcgcgctcg ccttaccgtc
     4081 gccgatcgca cggaggagta tgcggacgcc ggcggcccgg tccttctccc agggcggcgc
     4141 ctgtgtgacc agcgtgacga gatcgtcgaa gtcggcgcgt tcgaccgggg tcgagacacg
     4201 atccgcgagc gccgtcatcc ggagtcggcg attcgagttc tccgcggcac aggtggcgag
     4261 gaaggtctcg agcgcagcct tgccggctct cccctccgca aacttccgag cgaactcggc
     4321 gcgctggtcg aagcggatct ggcgcccggc gatggtcgcc cagaaacgct gctcgtcctc
     4381 gtcgaggtat ttgggatcga tccgctgcca ctcgcgttgg atcacgttca gtcgccgcgc
     4441 gacgtcgggc gtgaccggaa cgagccacgg tgccggcgag agccgacgcc tcgcccggcg
     4501 ctgagagcgt cgacggggag cgggggtcac ccgagcccct tctcggtaga agtcggagtc
     4561 gagcagagag tgcagggaga cgacgcgctc ggagtgtgtt gccgacttcg gaacgaagtc
     4621 gatcacgaca cccgcctcct tccgcgggtg gatccgcatg atgcgaccga tccgctgctg
     4681 gtagacccgt cgtgaggctg tcggcgccag atgcatgcag acggtcgcgc gcggagagtt
     4741 ccagccttcg gcaagcagct gcgcgttgat gaggacgttg atctcgccac gctcgtacgc
     4801 ggccagcgtc tcggcgagcc tgaccggcgg cgtccggccc gagaccgcct ccgcctgcag
     4861 cccggccgcc cggaactcct tggcgaggtt gtaggcgtgg tcgactccgg ccgcgtagac
     4921 gattccgggt gtagacgcaa accgatcgcg atagagactt gcggcagcct ggttcagcgc
     4981 ctggtggtcg agcgcgtcgg cgagggcgcg ctcctcgaaa tcgcctccga cgatcggaac
     5041 cgagttgatc gccgccgccg gcggaacacg gaggcagcgg agcggcgcaa tcagtccgcg
     5101 ccgggcggcg tcggcgagtg gaaggtcgtc caccgacgct gggaagacgt ccgagacctg
     5161 cttggcaatc agctgctccg tcgcggtcat cccgatgtag agcggctcgg gaagggcgcg
     5221 gatcgccgcg ctcgtcttct cgcccagagc cgtgtgggcc tcgtcacaga tcaccaggtg
     5281 atacgcggtg cggctcagcg actttccgtg gcgagcgaac caggcgtagg tctgcaccgt
     5341 gatcgggttg gccgtcgtgg ccgtcgcacc ggcctcgata gccccg
//
LOCUS       DBNGNK010000366         9315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1279352,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000366 DBNGNK010000000
VERSION     DBNGNK010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1279352"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC150V_10875"
     CDS             complement(<1..227)
                     /locus_tag="AC150V_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015234699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR3763461.1"
                     /translation="MTRAVVLSAVRTPIGRHGGGLAGVRPDDLAATAIAAAVERAGVE
                     PARIDDVFFGCANQAGEDNRNVARMAALLAGL"
     gene            complement(224..400)
                     /locus_tag="AC150V_10880"
     CDS             complement(224..400)
                     /locus_tag="AC150V_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002172718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HIR3763462.1"
                     /translation="MALELKPACERCQRRLPPDDVAFICSYECTFCAACVAELVGECP
                     NCGGELVRRPRRRP"
     gene            complement(466..1290)
                     /locus_tag="AC150V_10885"
     CDS             complement(466..1290)
                     /locus_tag="AC150V_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis family protein"
                     /protein_id="HIR3763463.1"
                     /translation="MLIRVGHSPDPDDAFMFWALTTDLVDRRGFEFEQVLQDIQTLNQ
                     WARAARLEVTALSLAAYPFVQQDYALLPHGASIGSGYGPVLVAREPLEVEELAGKEVV
                     VPGSLTTAALTLRLVLGDYRSRELPFEEIPEEISSGRAELGLLIHEGQLTFSDHGLFK
                     VLDLGEWWLLETGLPLPLGVNVARRDIGARLHDLSVVLADAIHCGLENRGEALDYALQ
                     FGRGIDVAVADRFIEMYVNELTQDYGDEGRKAVRELLERGEAIGAFPAPVTIDFVE"
     gene            1653..2462
                     /locus_tag="AC150V_10890"
     CDS             1653..2462
                     /locus_tag="AC150V_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015580887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein"
                     /protein_id="HIR3763464.1"
                     /translation="MTPFSDLFPPKPLIGMIHLPALPGAPRNESALEELVAFAVDEAG
                     KLERAGLDGAIVENVGDVPFFREDVPAATVAAMAIIVREVRAACALALGVNMLRNACT
                     QALSLAAVAGADFIRCNVVIGAYVTDQGIIQGCAAELARLRVTLDRDVLVLGDVHVKH
                     AHPLFDVPVEDAAADLAERGGADAVILSGARSPVPPTFERVERVRAAIDAPVLIGSGV
                     GSENAADYYALSDGLLLGEIDFKLDRVWGGASDETAYANAVSSCRSERPDT"
     gene            complement(2551..2934)
                     /locus_tag="AC150V_10895"
     CDS             complement(2551..2934)
                     /locus_tag="AC150V_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HIR3763465.1"
                     /translation="MIFAPAAMPTYALNFYSPTVADQLRTNRKTATIRLGDKSGKYRK
                     GMIVTVLVGVRYGQREKIFDAVIDKVEVKRLGDLSPREIEYDNPEIRRADELAHWLGQ
                     LYNREVTADDTVTVIRFSQIVGTWL"
     gene            complement(2967..3896)
                     /locus_tag="AC150V_10900"
     CDS             complement(2967..3896)
                     /locus_tag="AC150V_10900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR3763466.1"
                     /translation="MERVLVTGALGCLGAWVTRELTRGGAAVVGSDVGEDRSRLRALL
                     GDAELADVTLVTGDVTDLAALERLLAEHEITHVIHLAALQVPFCRENPPLGAAVNVVG
                     TTNLFEAVKRSTRVDRVVYASSMAVYGAADVGAENERTLPETHYGVYKVANEGTARIY
                     WQDDGVPSVGLRPYTVYGPTRDQGLTSTPTAAMLSASQGEPFHIPWGGVTWYQYARDV
                     AAAFIEAARAELDGARVYNLSGDRLHMSDVVAAIEAAVPSAAGSITFDDVQLPFPLDI
                     ETTRFAEEIAALQLTPFPEGVRETVAFGEKLAA"
     gene            complement(3912..4352)
                     /locus_tag="AC150V_10905"
     CDS             complement(3912..4352)
                     /locus_tag="AC150V_10905"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR3763467.1"
                     /translation="MRREFSAGGIVVRRMRSRWWLAAIRPRGKPEGTWTLPKGLIDEG
                     ETPVAAALRETSEETGLIARSVGKLGDSRYVYVWEGERVFKIVSFFLLRHRGGRIGEL
                     PAGMEVEVAEARWLPLDDAPRLLAYGGEREMARRAIDALTRAGT"
     gene            4503..4808
                     /gene="groES"
                     /locus_tag="AC150V_10910"
     CDS             4503..4808
                     /gene="groES"
                     /locus_tag="AC150V_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR3763468.1"
                     /translation="MNLQPLGDRLIVEVLEEEETTVSGIVLPDTAQEKPQRGKVLAVG
                     PGPRDEDGDYVKMDVEEGDEVIFSKYGGTEIKIGADEVLILRESDVLAKVVGDRAAV"
     gene            4834..6468
                     /gene="groL"
                     /locus_tag="AC150V_10915"
     CDS             4834..6468
                     /gene="groL"
                     /locus_tag="AC150V_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563800.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR3763469.1"
                     /translation="MAHKELKFDEAARRALERGVNTLADAVKVTLGPKGRYVVLDKKF
                     GAPTITNDGVTIAREIEVEDVFENQGAQLVKEVATATNDVAGDGTTTATVLAQAIVRE
                     GLKNVAAGANPMALKRGIEQAVESVVEDLKAQSTEVSGKEDIARVATISSREREIGDV
                     IADSIEKVGKDGVVNVEEGQTFGLDLEFTEGMQFDKGYLSPYMITDAERMEAVLDDPY
                     VLIANQKVGAVKDVLPVLEQVIQAGKPLLIVAEDVEGESLATIVVNKLRGTFTAVAVK
                     APGFGDRRKRMLEDIAILTGGEVITEEMGLKLENTKLSQLGRARKIVIDKDATTIIDG
                     AGDSDAIKGRIKQLKAEIETTDSDFDREKLQERLAKLSGGVAVVKVGAATETEMKEKK
                     HRVEDALQATRAALEEGIVPGGGVALLNAADAIKTSIDKLEGDERTGAQIIVRALEEP
                     LRQLAQNAGLEGSVVIDKVRSAKKGEGLNVDTGEIELLVKAGIIDPTMVTRSALQNAA
                     SIAKNILTTEAIVAEAPEKEAVGAGMPDMGGMGGMM"
     gene            complement(6527..7522)
                     /locus_tag="AC150V_10920"
     CDS             complement(6527..7522)
                     /locus_tag="AC150V_10920"
                     /inference="COORDINATES: protein motif:HMM:NF013117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR3763470.1"
                     /translation="MDATTFSLAAGARITQDEVAIDVTPCADDGVVCDWVYEATGSSW
                     AAESAAWLVSVPVTIILIVVVALVVNRIVRRLIHRTTESMASAARKGPPEFLASTQSR
                     ERAGERARALESILKATATAAIFVLATLLVLSNVGVNVVALLAGAGILGFALSFGAQS
                     LVKDVLAGISILIEDQYGIGDEVDIGYGPGTVEHVSLRSTAVRDYDGTIWYVPNGEIL
                     RVANFSQLWSRVRFDVRIAYESDLGRATEVIAAVAAELAAEQRFAGALREPPEPPIVQ
                     ELAPDAVVVRQIIWVRRPAVVPLERELRARTKEALQREGIGIGLPRHEISLVTAP"
     gene            complement(7809..8414)
                     /locus_tag="AC150V_10925"
     CDS             complement(7809..8414)
                     /locus_tag="AC150V_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020616122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HIR3763471.1"
                     /translation="MFRGRAEKKVRELGYEPSRLPPGQYLTEKWPVLHAGQVPTVDLA
                     TWDFRVFGAVENPITLSWEEFSRLESADVTLDIHCVTRWSRFDHAFSGVPWPVLAELV
                     RPRPGARFAIAHAEHDFTANVPLSRLEHEHALLATHSEGEPLALEHGGPLRLVVPGTY
                     FWKSAKWLRAIELAEEDRLGFWERYGYHNDADPFEEQRYSF"
     gene            8551..>9315
                     /gene="ygfZ"
                     /locus_tag="AC150V_10930"
     CDS             8551..>9315
                     /gene="ygfZ"
                     /locus_tag="AC150V_10930"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR3763472.1"
                     /translation="MSTSLRTATRPRGFLRVAGPDAASYLQRMVSNDVEALGPGEACE
                     ALLLTAKARVIAPMTVFRRGHEDFLLLTEPELAERVAGELRRFRFAAKAEIEHEAHDS
                     ALVLGEEPPEGAVPTADYGLPAYELLDVPAPRNANPIAEDELERLRILARTPRWGKEL
                     DDRVLPAEAGLDERAISFTKGCYPGQEPVARLHYRGHANRGLRVLSVEGDTSPEPDAE
                     LLFEGKSVGRVTSAVPDSDGAILALGYIRREVPSDAV"
BASE COUNT         1553 a         3183 c         3190 g         1389 t
ORIGIN      
        1 agaccggcga gcagtgcggc catgcgggcg acgttgcggt tgtcctcccc cgcttgattc
       61 gcacagccga agaagacgtc atcgatccgg gccggctcga cgccggctcg ctcgaccgcc
      121 gccgcgattg cggtcgcggc cagatcgtcc gggcgcaccc cggccagccc gccaccgtgg
      181 cgcccgatcg gcgtcctgac agcggagagg acgacggcgc gcgtcacggc cgccggcgag
      241 gtcgccggac gagctcgccg ccgcaattcg ggcactcgcc gacgagctca gcgacgcatg
      301 cggcgcagaa ggtgcattcg tagctgcaga tgaacgcgac gtcgtcgggc ggtagcctgc
      361 gctggcaccg ctcgcaggcc ggtttcagct cgagcgccat gcgcgcaggc tagtcgatcg
      421 cccgcccgct tcccacgcag gctcgacggc cggggtgcca cacgtctact cgacgaaatc
      481 gatcgtgacg ggagccggga aggcgccgat cgcctcgccg cgctccaaca gctctcgcac
      541 ggccttccgc ccttcgtcgc cgtagtcctg ggtcagctcg ttcacgtaca tctcgatgaa
      601 gcggtcggcc accgcgacat cgattccacg cccgaactgc aacgcgtagt cgagcgcctc
      661 gccgcggttc tccaaaccgc agtgaatcgc gtcggcgagc acgacggaga gatcgtggag
      721 gcgcgcgccg atgtcacgac gagcgacgtt gacaccaagc ggcaagggga ggcccgtctc
      781 gagcagccac cactccccga ggtcgagaac cttgaacagt ccgtgatccg agaacgtcag
      841 ctggccctcg tggatgagga gcccgagctc ggcccggccc gaggagatct cctccgggat
      901 ctcctcgaac ggcaactcgc gcgaccgata gtcacccagc accagccgca atgtgagcgc
      961 cgcggtggtg agcgaccctg gaaccacgac ttccttcccg gcaagctcct ccacctcaag
     1021 gggctcgcgt gcgaccagca cgggcccgta gccggagccg atgctcgcgc cgtgcggaag
     1081 cagcgcgtaa tcctgctgca cgaacgggta ggccgccagc gacagcgccg tcacctcgag
     1141 tcgagcggcg cgcgcccact ggttgagcgt ctggatgtcc tgcagcacct gctcgaactc
     1201 gaacccccgt ctgtccacga ggtcggttgt cagcgcccag aacatgaacg cgtcgtcagg
     1261 gtcgggactg tggccgacgc ggatcagcac gagcgcagga gcctaacgac ccttttcacg
     1321 cggcgaggag agaaccggcg gccgtagact cactcccatc gaaggatcac cgacctgagc
     1381 cgaccccgcc gcaactggat ccaggaagaa cgccgccgca cgctggggga ctgggtggcg
     1441 ttctgcctct cgtgcggcca cacgctgcgc tacttcgagc agttcgacca cgacctgccc
     1501 ggctcatgcc cgcagtgccg aggagagctg aggagcgagt gtccttcgtg tggtgcccgc
     1561 ttcgcgtcgg ccttccaggt cgaatgtgag gaatgcggtg cgccggtgcg tccgcccgac
     1621 ctgttcggtg ggccgatcag gaaggccggt cgctgacccc gttctccgat ctcttccccc
     1681 cgaagcccct gatcgggatg atccatctgc cggccctacc gggcgcaccg cggaacgagt
     1741 cggcgctcga ggagctggtc gccttcgccg tcgacgaggc cgggaagctc gagcgggcag
     1801 gactcgacgg cgcgatcgtc gagaacgtcg gcgacgtccc gttcttccgt gaggacgtcc
     1861 cggccgccac ggtggcggca atggcgatca tcgtccgcga ggtgcgggct gcctgcgcgc
     1921 tcgctctcgg cgtcaacatg ctccgtaacg cctgcaccca ggcgctctcg ctggcggcgg
     1981 tcgcaggagc cgacttcatc cgctgcaacg tcgtgatcgg cgcttacgtc accgaccagg
     2041 gcatcatcca gggctgcgcc gcggagctcg cacgtctccg agtcacactc gatcgcgacg
     2101 tcctcgtcct cggcgacgtg cacgtcaagc acgcgcatcc gctgttcgat gtccctgtcg
     2161 aggacgcggc ggcggatctg gccgagcggg gcggcgccga cgccgtgatc ctgtccggcg
     2221 cccgaagtcc ggttccgccc accttcgagc gggtcgagcg cgtccgcgct gcgatcgacg
     2281 ctcccgttct gatcggaagc ggagtcggca gcgagaacgc agccgactac tacgcgctct
     2341 ccgacggcct cctgctcggc gagatcgact tcaagctcga ccgggtctgg ggcggtgcga
     2401 gcgacgagac ggcctatgcg aacgccgtct cgagctgtcg ctccgagcgc ccggacacct
     2461 agccacgcgc ggtcggatac cgcccccggg ctgggtggcg cccggatcgc cgcacgaaac
     2521 cacggtcgcg cgcccgccgt tcccccacga tcaaagccag gtgccgacga tctgggagaa
     2581 gcggatcacg gtcaccgtgt cgtccgccgt gacctcccgg ttgtagagct gtccgagcca
     2641 gtgcgccagc tcgtctgcgc gcctgatctc ggggttgtcg tactcgatct cgcgcggcga
     2701 cagatcaccg agccgcttga cctccacctt gtcgatgacg gcgtcgaaga tcttctcgcg
     2761 ctgcccgtag cgaacaccta cgaggacggt cacgatcatg cccttgcggt acttgcccga
     2821 cttgtcgccg agtcggatgg tggcggtctt ccgattcgtg cggagctgat cggcgaccgt
     2881 cggcgagtag aagttgaggg cgtacgtcgg catcgcggct ggcgcgaaga tcatacgggc
     2941 cggatcggtt ttcgtcggcg atccggctac gccgcgagct tctcgccgaa cgcgacggtc
     3001 tcccgcacgc cctccgggaa gggtgtgagc tgcagcgcgg caatctcctc ggcgaagcgg
     3061 gtggtctcga tgtcgagcgg aaacgggagc tggacgtcgt cgaaggtgat ggatccggcc
     3121 gccgacggga ccgccgcctc gatcgccgca accacgtcgc tcatgtgcag acggtctccc
     3181 gacaggttgt agacccgcgc tccgtcgagc tcggcgcgcg cggcctcgat gaaggctgcg
     3241 gccacgtccc tggcgtactg gtaccaggtg accccgcccc acgggatatg gaacggctcg
     3301 ccctgcgagg cggacagcat cgccgccgtg ggcgtcgagg tgaggccctg atcgcgagtc
     3361 gggccgtaga ccgtgtaggg ccggaggccg acactcggca cgccgtcgtc ctgccagtag
     3421 atgcgcgcgg tgccctcgtt ggcgaccttg tagacgccgt agtgggtctc gggcagcgtt
     3481 ctctcgttct cggctccgac gtcggccgcg ccgtagaccg ccatgctgga cgcgtagacg
     3541 acgcggtcga cgcgcgtgct ccgcttgacg gcctcgaaga ggttcgtggt accgacgacg
     3601 ttcaccgcag ccccgagcgg cgggttctcc ctgcagaagg gcacctgtag cgccgcgagg
     3661 tggatcacgt gggtgatctc gtgctccgcg agcagccgtt cgagcgccgc gagatcggtg
     3721 acatcgccgg tgacgagcgt cacgtcggcg agctctgcgt cgcccagcaa cgctcgcagc
     3781 cgcgagcggt cctcgccgac gtcgctgccg acgacggccg cgccgccgcg ggtcagctca
     3841 cgcgtgaccc aggcgccgag gcagccgagc gcgccggtga cgagaacgcg ttccacgcgg
     3901 cgattctcgc atcacgtgcc tgcacgggtg agcgcatcga tcgcccgccg ggccatctcc
     3961 cgctcgccgc cgtaggcgag cagccgtggc gcgtcgtcga gcggcagcca ccgcgcctcg
     4021 gcgacctcga cctccatccc ggcgggcagc tcgccgatcc tgccgccgcg gtgccgaagc
     4081 aggaagaacg acacgatctt gaagacgcgc tcgccctccc agacgtagac gtaccgggag
     4141 tctccgagct tgccgaccga ccgtgcgatc aggccggtct cctcactcgt ctcgcggagc
     4201 gcggcggcga ccggcgtctc gccctcgtcg atcagtccct tcggcaaggt ccatgtgccc
     4261 tcgggcttgc cgcgcggtcg tatcgccgcg agccaccagc ggctgcgcat gcggcgcacg
     4321 acgatgccgc cggccgagaa ctcccgcctc atcttctaca catcagtcgc tctcaagatc
     4381 ttagtgcctt cagctaaggg tagggttccc ctgcgacgcc agctgtgcta ttgtcccgct
     4441 ggcactcagc aacggagagt gccagatccc tgtcgtaatc acagtccata ggaggaatgt
     4501 ggatgaattt gcagccgctg ggtgaccgcc tgatcgtgga agtcctcgag gaggaggaga
     4561 ccacggtgag cggcatcgtg ctgcccgaca ccgcccagga gaagccgcag cgcggcaagg
     4621 tcctggctgt cggccccggc ccgcgcgacg aggacgggga ctacgtcaag atggacgtcg
     4681 aggagggtga cgaggtcatc ttctccaagt acggcggcac cgagatcaag atcggcgccg
     4741 acgaagtcct gatcctgcgc gagtcggacg tcctggccaa ggtcgtcggc gaccgcgcgg
     4801 ccgtctaaga gaacacagga ggaaccccga gagatggctc acaaagagct caagttcgac
     4861 gaggccgcgc gccgcgcgct cgagcggggc gtcaacaccc tcgccgacgc cgtcaaggtg
     4921 acgctcggcc cgaagggccg ctacgtcgtg ctggacaaga agttcggcgc gcccacgatc
     4981 accaacgacg gcgtcacgat cgcccgcgag atcgaggtcg aggacgtctt cgagaatcag
     5041 ggcgcacagc tggtcaagga agtcgcgacg gcgacgaacg acgtcgccgg cgacggaacg
     5101 acgactgcca cggtcctcgc ccaggcaatc gtccgcgagg gcctgaagaa cgtcgcagcc
     5161 ggtgccaacc cgatggcgct gaagcggggc atcgagcagg ccgtcgagtc ggtcgtcgaa
     5221 gacctgaagg cgcagtcgac cgaggtgtcc ggcaaggagg acatcgcccg cgtcgccacc
     5281 atctcctcgc gcgagcggga gatcggcgac gtgatcgcgg actcgatcga gaaagtcggc
     5341 aaggacggcg tcgtgaacgt cgaggagggg cagacgttcg gcctcgacct cgagttcacc
     5401 gagggtatgc agttcgacaa gggctacctg tcgccgtaca tgatcaccga cgccgagcgc
     5461 atggaggccg tgctcgacga cccgtacgtc ctcatcgcca accagaaggt cggcgccgtg
     5521 aaggacgttc tgcccgtgct cgagcaggtc atccaggcgg gcaagccgct gctcatcgtg
     5581 gcggaggacg tcgagggcga gtcgcttgcg acgatcgtcg tcaacaagct gcgcggcacg
     5641 tttaccgccg tcgcggtcaa ggcaccgggc ttcggtgacc gccgcaagcg catgctggag
     5701 gacatcgcga tcctcaccgg cggcgaggtg atcaccgagg agatgggcct caagctcgag
     5761 aacacgaagc tctcgcagct cggtcgggcc cggaagatcg tcatcgacaa ggatgcgacc
     5821 acgatcatcg acggtgccgg cgactccgac gccatcaagg gtcggatcaa gcagctcaag
     5881 gcagagatcg agaccaccga ctccgacttc gaccgcgaga agctgcagga gcggctggcc
     5941 aagctctcgg gcggcgtcgc agtcgtgaag gtcggcgctg cgaccgagac cgagatgaag
     6001 gagaaaaagc accgcgtcga ggacgccctg caggccaccc gcgcggcgct cgaggagggc
     6061 atcgtgcccg gtggcggcgt cgcgctgctg aacgcggccg atgcgatcaa gacctccatc
     6121 gacaagctgg agggcgacga gcggaccggg gcgcagatca tcgtccgggc gctcgaggag
     6181 ccgctacgcc agcttgcgca gaatgctggc ctcgagggct cggtcgtcat cgacaaggtc
     6241 cgtagcgcca agaagggcga gggcctgaac gtcgacacgg gcgagatcga gcttctcgtc
     6301 aaggccggga tcatcgatcc gacgatggtc actcggtcgg cgctgcagaa cgccgcgtcg
     6361 atcgcgaaga acatcctgac caccgaggcg atcgtcgccg aggcgccgga gaaggaagct
     6421 gtcggcgccg gcatgccgga catgggcgga atgggtggaa tgatgtaacg agcccccggg
     6481 ctaatacccg tgcgaggggt cggcgagagc cggcccctcg tcgtttctag ggggccgtca
     6541 cgagcgagat ctcgtggcgc ggaagaccga ttccgatgcc ctcccgctgg agcgcctctt
     6601 tcgtccgagc gcgaagctcg cgctccaggg gcaccacggc gggcctgcgc acccagatga
     6661 tctgcctgac gacaaccgcg tcgggcgcga gctcctgcac gatcggcggc tccggcggtt
     6721 cccgcagcgc tccggcgaag cgctgctcgg cagcgagctc ggctgccacg gctgcgatca
     6781 cctcggtcgc ccgcccgaga tcggactcgt aggcgatgcg gacgtcgaag cgcacgcgcg
     6841 accacagctg cgagaagttc gcaacacgaa gaatctcgcc gttcggcacg taccagatcg
     6901 ttccgtcgta gtcgcgcacc gccgtcgagc gaaggctcac gtgctcgacc gttccgggtc
     6961 cgtagccgat atcgacctcg tctccgatcc catattggtc ctcgatcagg atcgagatcc
     7021 cggcgagcac gtccttgacg agcgactgcg cgccgaagct gagcgcgaag ccgaggatgc
     7081 ccgcgccggc gagcagggcg acgacgttca cgccgacgtt gctgagcacg agcagggtcg
     7141 cgaggacgaa gatggctgcc gtagcggtcg ccttcaggat gctctcgagt gcgcgggcac
     7201 gctcaccggc ccgttcgcgc gactgggtgg aggcgaggaa ctcgggcggg cccttccgtg
     7261 cggcgctcgc catcgactcc gtcgtgcggt ggatcaggcg gcgcacgatc ctgttcacga
     7321 caagcgccac gacgacgatc aggatgatcg ttaccgggac gctgacgagc caggccgcac
     7381 tctcggcagc ccatgagctc cccgtcgcct cgtacaccca gtcgcacact acgccgtcgt
     7441 cggcgcaagg ggtgacgtcg atcgcgacct cgtcctgagt gatccgggcg cctgcggcga
     7501 gggagaacgt cgtcgcgtcc atcccaggac agttcggggt acggcgccgt cgatcctgca
     7561 cggcagactg cgcggctggc cgccgggcga agcgcggctc cgcggccgca gggtgatgta
     7621 caggagagag gcgccccgaa acagtccccg aacgtgaaag cgaccgctgg ggggcggccg
     7681 ctttcctggg attggaactg ggggagggtg ggtgcaccta gtttcggcgg gaccggccgc
     7741 tgcctcgtcc ctcttccggg tgatcttcgc ctcactcgga cggggcggcc taccgtcccg
     7801 caccggcttc agaagctgta gcgctgctcc tcgaacggat cggcgtcgtt gtggtagccg
     7861 tagcgctccc agaagccgag ccggtcctcc tcggcgagct cgattgcccg aagccatttc
     7921 gcgctcttcc agaagtacgt gccgggaacg acgagacgga gcggcccgcc gtgctcgagc
     7981 gcaagcggct cgccttccga atgcgtggcg agcaaggcgt gctcgtgctc gagcctcgag
     8041 agtggaacat tcgccgtgaa gtcgtgctcg gcgtgcgcga tcgcaaaacg ggcccccggc
     8101 ctgggccgga cgagctcggc gagcaccggc caggggacgc cgctgaacgc atggtcgaag
     8161 cggctccagc gcgtcacgca atggatgtcg agcgtgacgt cggcgctctc gaggcgagag
     8221 aactcttccc agctcaacgt gatcgggttc tcgacggctc cgaagacgcg gaagtcccac
     8281 gtcgcgaggt cgacggtcgg cacctgcccc gcgtggagca cgggccactt ctccgtcaaa
     8341 tactggcccg gagggagcct tgacggctcg tagccgagct cccgcacctt cttctcggct
     8401 ctgcctctga acatgcgtcc cacagtagcc acgcccagga cgtgacgggc cgcctccctg
     8461 ctttgctccg ctcatacact gaggactgag cggttcggag cactcgtgaa gcgggacgct
     8521 cgctcgtagc gcgctcccta cactgaccgc atgagcacct cgctgcgcac ggcgacgcgg
     8581 ccacgcggct tcctgcgcgt ggccggtccg gacgcggctt cctacctgca gcgaatggtc
     8641 tcgaacgacg tcgaggcact ggggcccggc gaggcctgcg aggcactgct gctgaccgcg
     8701 aaggcgcgag tgatcgcgcc gatgacggtg ttccgacggg ggcacgagga cttcctcctg
     8761 ctgacggagc ccgagctcgc cgagcgtgtc gcgggcgagc tgaggcgctt tcgcttcgcc
     8821 gcgaaagcgg agatcgagca cgaggcgcac gactcggcgc tcgtgctcgg cgaagagcct
     8881 ccagaaggcg cggttccgac cgccgactac ggactaccgg cgtacgagct gctcgacgtg
     8941 cccgcgccac gcaacgccaa cccgatcgcg gaggacgagc tcgagcggct gcgcatcctc
     9001 gcgcgaacgc cgcgctgggg gaaggagctc gacgaccgcg tgctcccggc cgaggctggc
     9061 ctcgacgagc gggcgatcag cttcacgaag gggtgctatc cgggacagga gcccgtggcg
     9121 cggctccact accgcggcca cgcgaacaga ggcctgcgcg tcctctcggt cgagggagac
     9181 acgtcgcccg agcccgatgc cgagctgctg ttcgagggga agagcgtcgg ccgcgtcacg
     9241 agcgctgttc cggactccga cggggcgatc ttggcactcg gttacatccg gcgcgaggtg
     9301 ccctcggacg ccgtc
//
LOCUS       DBNGNK010000367         6499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_64833,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000367 DBNGNK010000000
VERSION     DBNGNK010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_64833"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="AC150V_10935"
     CDS             <1..187
                     /locus_tag="AC150V_10935"
                     /inference="COORDINATES: protein motif:HMM:NF012626.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR3763473.1"
                     /translation="VPTRVLEEVERVNEDLAGRGRVLVRPSGTEPVVRVLAEAQTEAE
                     AAALCARIAHLVGEDSG"
     gene            267..2099
                     /gene="glmS"
                     /locus_tag="AC150V_10940"
     CDS             267..2099
                     /gene="glmS"
                     /locus_tag="AC150V_10940"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR3763474.1"
                     /translation="MCGIIGYVGSREARPLLLQGLERLEYRGYDSAGIALLEGEGLRY
                     TRAVGNLQNLKYAVGDGESAATTGLGHTRWATHGGVTLQNAHPLAAEEPERLSVVLNG
                     IVENYRELRERLQAGGHAFSSETDAETVTHLIEEHYEGDLVEATRRAFNDLEGHFAFV
                     VIHRDHPGELVGARHQCPLVVGVTDGEAFFASNAAAFLSETRDVFFPDDGQIAHVTSA
                     GVVFLSAADGSAVENELVTLDWDEESAEKGGYETFMLKEIFEQPDAVAETIGDRLRHG
                     KLELEGLGMSDEQLCGLQRIVILSAGTSYHAGVASRYAIEEWARVPVEHDIASEWIYR
                     YPVLDENTLVIGISQSGETRDTISAMQLAREMGAHTVAITNMMGSQITREVDSVLYTR
                     AGIEVSVAASKTFTAQLALLFLVALKLAQVRETLPAGELEFIVNQVYSLPTKIRRFLE
                     GDHPVEEIARRFYDEPFFLYLGRNIGLPVALEGALKLKEISYIPTEAYSAGEMKHGPI
                     ALLGENTPVVVVATYHKRVYDKIVSNIQEVRARGAHVIAIATDGNEDIQHHADDVIYV
                     PKTPAFLQAALAIVPLQLLAYRIARLRGLNVDQPRNLAKTVTVE"
     gene            2185..2877
                     /locus_tag="AC150V_10945"
     CDS             2185..2877
                     /locus_tag="AC150V_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763475.1"
                     /translation="MAPRIVVIVLSTVLALAVAGSALARTPHDTTYKPVGRDVRTAKG
                     LLVKRADVPGFADAGAPDDRDATCSTYDPDLSGLVTTGEADGRYYSRTSASGALTVGS
                     QAALFRSAAHAGSYWKRAVTSKKITPCLSEVFRRGLPPGAKLSNVRTVPVAAASGALR
                     VHSWNLVGRVHVDGNVVPFVLEAVGLKRGRAISFVFAMSVGTPLAEQVRRVGAVASAK
                     LLRAKLGTAPAS"
     gene            complement(2903..4309)
                     /locus_tag="AC150V_10950"
     CDS             complement(2903..4309)
                     /locus_tag="AC150V_10950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR3763476.1"
                     /translation="MARYDLVIVGLGSAGFIATEVASALGLRVAAVEAARPGGDCLWT
                     GCVPSKALVAAAKAAYVMRTADRFGIPSVEPDVDLRAVWERIRSLQEEIASTDDSPER
                     FEQLGVELIQGRARLAGPTTVDVSGRVLDTRFVLLATGSRPAVPAVDGLAEARYLTSE
                     SLWFVDTPPRSVVVIGGGPMGVELAQGLNRLGVGTTLLQKGSRLLPRDEPELAEIVAR
                     KLAAEGVDVRLDADVRRVELDGGAKTVAARVGGDEHRWAVEEIIVATGRRPNVDGLGL
                     SELGIELTDRGVVTDDALRTSVDSVYAVGDVAGRHRFTHSAAYEASIALRNMFFPWRS
                     KANALIPWTTFTDPPLASAGLTAEAAVERYGARTVRVWRQDMVESDLARVDAEVDGAM
                     LLVTAKGRLVGAHVLAPAAGELMHELALAIDKRMRLRDLAFQVHIYPSVALSAQRLAG
                     FSMYELVRRLRVLVRRAR"
     gene            complement(4314..5429)
                     /locus_tag="AC150V_10955"
     CDS             complement(4314..5429)
                     /locus_tag="AC150V_10955"
                     /inference="COORDINATES: protein motif:HMM:NF022818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HIR3763477.1"
                     /translation="MSRRLIALLVAGFLLLLGLAGAGALLTIGNGQELSRAGDPTDEE
                     FDAIVERIDEVAPLVGLGEASPAPPDERFWDTNFDKALVPLTEFRSGGPPKDGIPALD
                     KPAFETAVSVRWLKNREPVIAVTVDGETRGYPLQIMTWHEIANDSIQGVPVAVTFCPL
                     CNTAIVFDRRVDGKTLDFGVSGKLRNSDLVMFDRQTESWWQQFEGKALVGDLAGKKLV
                     QLPARIVSWKEFRTTYPESRVLSRKTGHGRDYGRNPYTGYDDIASPPWYGARNDGDKR
                     LPPKERVVFLQVGSAAIAVPFSALAKKKLVRTTAAGKKIVIRWRGGIASALDSEKIWN
                     GRDVGAAQVTVDGRPAPFSEPFWFGVAAFRPDTRIVR"
     gene            complement(5419..>6499)
                     /locus_tag="AC150V_10960"
     CDS             complement(5419..>6499)
                     /locus_tag="AC150V_10960"
                     /inference="COORDINATES: protein motif:HMM:NF022818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HIR3763478.1"
                     /translation="GDTVEAEPGVAEPDLRRDLGVNAAFLRDFDPEALSVGAGIDVIP
                     AIDDPVFETPEEVTLVSPSDLVVGVEHDGDARAYPTKLLSLHEVVNDEIGGRPVVVTW
                     CPLCQTALAFDRRVDGETLRFGVSGWLYSANLLLFDRTSGSLWSQLLGGAVTGSYRGT
                     ELEPVPVVHETWEAWLAAHPDTKVLSVAADEFSEFFTDETIEATSRGEAISSAPYAEY
                     TQKVKTYFDREIDGIPGAERMLGVVAGPASRAYRLLTLQQRRVIEDTVGGTPIVVTWD
                     PEAVSANAFSRLLDDERVLSFRRDGDELVDAETGSRWSPRLGTALSGPLEGTRLERAP
                     ATFSYWFAWSRIYPGGSVYPASDEP"
BASE COUNT         1047 a         2193 c         2247 g         1012 t
ORIGIN      
        1 ggtgccgacg cgcgtgctgg aggaggtcga gcgggtcaac gaagacctcg ccggccgcgg
       61 ccgcgtgctc gtacggccct ccggtaccga gccggtcgtt cgcgtcctcg ccgaggccca
      121 aacggaggcc gaggcagctg ccctctgtgc taggatcgcg catctcgttg gagaggattc
      181 cgggtagacc tggcacgtct atcgacgttc aatgagtcag acccctcgtc cgcggcaggc
      241 ggggcagcga ggaggagtct cgaacaatgt gcgggatcat cggatatgtc ggttccaggg
      301 aggcgcggcc gctgctgctg caggggctgg agcggcttga gtaccgtggc tatgactcgg
      361 cggggatcgc tctgctggag ggcgaggggc ttcgttatac gcgggccgtg gggaatctcc
      421 agaacctgaa gtacgcggtc ggcgacggcg agtcggcggc tacgaccggg ttggggcata
      481 cgcgttgggc gacgcacggc ggggtcacgc tgcagaacgc gcatccgctg gcggccgagg
      541 agcccgagcg gctgtcggtc gtcttgaacg gcatcgtcga gaattaccgt gagctgcgcg
      601 agcgactgca ggcgggcggc cacgcgttct cgtccgagac cgacgcggag acggtcacgc
      661 atctgatcga ggagcattac gagggcgacc ttgtcgaggc gacgcgtcgt gctttcaacg
      721 acctggaggg ccatttcgcg ttcgtcgtga tccaccgcga ccatcccggg gagctcgtcg
      781 gggcacggca tcagtgccca ctcgtggtcg gtgtcacaga cggcgaggcg ttctttgcct
      841 cgaacgcggc ggcgttcctc agcgagaccc gcgacgtgtt cttccccgac gacgggcaga
      901 tcgcgcatgt gacgtcggcc ggggtcgtgt ttctgagcgc tgccgacggc tcggccgtcg
      961 agaacgagct cgtgacgctc gactgggacg aggagagcgc cgagaagggc ggctacgaga
     1021 cgttcatgct gaaggagatc ttcgagcagc cggatgcggt cgccgagacg atcggcgaca
     1081 ggctgcgcca cggcaagctc gagctggaag ggctggggat gagcgacgag cagctttgtg
     1141 ggctgcaacg catcgtcatt ctctcggccg gaacgtcgta tcacgccggt gtggcgagcc
     1201 gttacgcgat cgaggagtgg gcccgcgttc cggtggagca cgacatcgcg agcgagtgga
     1261 tctatcgcta cccggtgctc gacgagaaca cgctcgtcat cggcatcagc cagtcgggcg
     1321 agacgcgcga cacgatctcg gcgatgcagt tggcgcgcga gatgggcgcg cacaccgttg
     1381 cgatcacgaa catgatgggc tcgcagatca cccgcgaggt cgactcggtt ctgtacacgc
     1441 gcgccggcat cgaggtcagc gtcgccgcgt ccaagacctt caccgcgcag ctcgcgctcc
     1501 tcttcctcgt cgcgctcaag ctggcgcagg tcagggagac gctgccggcg ggcgagctcg
     1561 agttcatcgt caaccaggtg tactcgctgc cgacgaagat ccggcgcttc ctcgagggcg
     1621 accaccccgt ggaagagatc gcacgccggt tctacgacga gccgttcttc ctctacctcg
     1681 gccgtaacat cggcctgccc gtggcgctcg agggcgcgtt gaagctgaag gagatctcgt
     1741 acattccgac ggaggcgtac tcggccggcg agatgaagca cggcccgatc gcgctgctcg
     1801 gcgagaacac gccggtcgta gtcgtggcga cgtaccacaa gcgcgtctac gacaagatcg
     1861 tctcgaacat ccaggaggtt cgagcgcggg gcgcgcacgt gatcgcgatc gcgaccgacg
     1921 ggaacgagga catccagcac catgcggacg acgtcatcta cgtgccgaag acacccgcct
     1981 tcctgcaggc cgcactcgcg atcgtgccgt tgcagctgct cgcgtaccgg atcgcgcgcc
     2041 tgcgcggact gaacgtcgac cagccccgca acctcgccaa gaccgtcacc gtcgagtagc
     2101 cgcatcgcgc caacccgtcg agggcacgaa ggccccccag tagggggaat gcgccagccc
     2161 gcctgcgctc gtaccgtgga agagatggcc ccgcgaatcg tcgtcatcgt gctgtccacg
     2221 gtcctcgcgc tcgccgttgc agggtctgca ctcgctcgca cgccgcacga cacgacatac
     2281 aagcctgtcg gtcgcgacgt gcgcacggca aagggcctgc tcgtcaagcg cgcggacgtc
     2341 cccggcttcg ccgacgccgg cgcgccggac gaccgtgacg ccacctgctc gacgtacgac
     2401 cccgacctca gcgggctcgt gacgaccgga gaggccgacg ggcgctacta ctcgcgcacg
     2461 tccgcctcgg gagcgctcac cgtcggctcg caggctgcgc tcttcaggag cgccgcccac
     2521 gccggttcct actggaagcg cgcggtcacg agcaagaaga tcaccccgtg tctgagcgag
     2581 gtcttccgtc gcggccttcc gccgggcgcg aagctcagca acgtgcgaac ggtccccgtg
     2641 gccgccgcct cgggtgcgtt gcgcgtgcat tcctggaacc tggtcggccg ggtgcacgtc
     2701 gacggcaacg tcgtcccgtt cgtcctcgag gcagtcgggc tgaaacgcgg gcgcgcgatc
     2761 tccttcgtct tcgcgatgag cgtcggcacg ccgctcgccg aacaggtaag gcgggttggt
     2821 gcggtcgcca gcgccaagct gttgcgcgcg aagctcggca cagcccctgc ctcctagagg
     2881 cgcgcagcgc ggacgccgta cgctaccgcg ctcgccgtac gaggacgcgc agccggcgga
     2941 ccagctcgta catcgagaat ccggcgagcc gttgcgccga cagggcgacg ctcgggtaga
     3001 tgtggacttg gaaggcgaga tcccgcagcc gcattcgctt gtcgatcgcg agcgcgagct
     3061 cgtgcatcag ctcgccggcg gccggtgcaa ggacgtgggc gccaacgagg cggcccttcg
     3121 ccgtcacgag cagcatcgcg ccgtccacct ctgcgtcgac acgggcgaga tccgattcga
     3181 ccatgtcctg acgccacacc cgcaccgtcc tcgctccgta gcgctcgacg gcggcctctg
     3241 cggtcaagcc ggcgcttgcg agcggcgggt cggtgaaggt cgtccagggg atcaacgcgt
     3301 tcgctttcga gcgccagggg aagaacatgt tgcggagggc gatgctcgct tcgtaggcgg
     3361 ctgagtgcgt gaagcgatgg cgtccggcga cgtccccgac ggcgtagacg gagtccacgc
     3421 tcgtgcgcag ggcgtcgtcg gtgacgacac cgcggtcggt gagctcgatg ccgagctcgc
     3481 tcaggccgag tccgtccacg ttcgggcgtc gcccggttgc gacgatgatc tcttcgaccg
     3541 cccagcggtg ctcgtcgcct ccgacgcggg ccgccacggt cttggcgccg ccgtcgagct
     3601 cgacgcgccg gacgtcggca tcgagtcgaa cgtcgacccc ctcggccgcg agcttgcggg
     3661 ccacgatctc cgccagttcc ggctcgtcgc ggggcaggag ccgcgagccc ttctgcagca
     3721 gcgtcgttcc gacaccgagc cggttgaggc cctgcgccag ctcgacgccc atcggcccgc
     3781 cgccgatgac gacgacgcta cggggtgggg tatccacgaa ccagagcgat tcgctcgtca
     3841 aatagcgggc ctcggccagg ccgtcgacag ccggcactgc cggccggctg ccggtcgcca
     3901 acagaacgaa gcgcgtgtcg aggacgcgcc cggacacgtc gactgtcgtc ggtccggcga
     3961 gacgcgcgcg cccttggatc agctcgaccc cgagctgctc gaaccgctca gggctgtcgt
     4021 ccgtcgacgc tatctcctcc tggagcgagc ggatccgctc ccagaccgcc cggaggtcga
     4081 cgtcgggctc gacactcggg atgccgaatc ggtcggcggt ccgcatcacg taagccgcct
     4141 ttgccgcggc cacgagcgcc ttactcggaa cgcaacccgt ccagaggcag tctccgcccg
     4201 gtcgcgccgc ctcgacggcg gcgacccgca ggccgagcgc ggaagcgacc tcggtggcga
     4261 tgaagcccgc cgagcccaag ccgacgatca cgaggtcgta gcgagccaag cggttaacgg
     4321 acgatgcgcg tgtcgggtcg gaaggcggcc actccgaacc agaacggctc ggaaaagggt
     4381 gccggtcggc cgtcgaccgt cacttgcgcg gcaccgacat ctcggccgtt ccagatcttc
     4441 tcggagtcga gcgcggaggc gattccgcca cgccaacgga tgacgatctt cttgcctgcc
     4501 gcggtggtgc gcacgagctt cttcttcgcc agtgccgaga acggcaccgc gatcgcggcg
     4561 ctgccgacct gcaggaagac gacgcgctcc ttcggcggga gccgcttgtc gccgtcgttg
     4621 cgggctccgt accagggcgg ggacgcaatg tcgtcgtagc ccgtatacgg gttgcgtccg
     4681 tagtcgcgac catgacccgt ctttcgggaa agaaccctgc tttccggata cgtcgtccgg
     4741 aactccttcc aggagacgat gcgagccggg agctgcacga gcttcttccc ggcgaggtcg
     4801 ccgaccagcg ccttcccctc gaactgttgc caccacgact ccgtctggcg atcgaacatg
     4861 acgagatcgg agttgcggag ctttcccgag acgccgaaat cgagcgtttt cccgtcgact
     4921 ctgcgatcga acacgatcgc cgtgttacag agagggcaga acgtgacggc gaccgggacg
     4981 ccctggatcg agtcgttggc gatctcgtgc caggtcatga tctgcagcgg gtagccgcgg
     5041 gtctcgccgt cgacggtaac ggcgatgacg ggctcccggt tcttcagcca ccgcacgctc
     5101 accgccgttt cgaaagcggg cttgtcgagc gccggaatgc cgtccttggg tggcccgccg
     5161 gagcggaact ccgtcagcgg aaccagtgcc ttgtcgaagt tggtgtccca gaagcgttcg
     5221 tcgggcggtg ccggagacgc ttcgccgagg ccgacgagtg gcgcgacctc gtcgatccgc
     5281 tcgacgattg cgtcgaactc ctcgtcggtt gggtctccgg cccggctgag ctcctggccg
     5341 tttccgatcg tgagcagcgc ccctgcgccc gccaggccga gaaggagcag gaagccggcc
     5401 acgaggagcg cgatcagcct acggctcatc ggaagcaggg tagacggatc cccccgggta
     5461 gatcctgctc caggcgaacc agtaggagaa cgtcgccggc gctcgctcga gccgagtgcc
     5521 ttcgagtggc ccgctgagag ccgtgccgag ccgcggcgac cagcgtgagc cggtctccgc
     5581 gtcgaccagc tcgtcgccgt ctcggcggaa cgagagcacg cgctcgtcgt cgagcaagcg
     5641 tgagaaggca ttggccgata ccgcctccgg atcccaggtg acgacgatgg gcgtgccgcc
     5701 gaccgtgtcc tcgatcacgc gccgctgctg gagggtgagc aggcgatagg cgcgcgaggc
     5761 ggggccggcg accacgccga gcattcgctc ggcacccggg atcccgtcga tctcgcggtc
     5821 gaagtacgtc ttcaccttct gcgtgtactc ggcgtagggg gcggaggaaa tcgcctcgcc
     5881 ccgactggtc gcctcgatcg tctcgtcggt gaagaactca gagaactcgt cggccgcgac
     5941 ggacagcacc ttcgtatcgg ggtgtgcggc gagccacgcc tcccaagtct cgtgaacgac
     6001 gggcaccggc tcgagctcgg taccccggta cgagccggtc accgcgccgc ccagcagctg
     6061 cgaccagagg ctgcccgacg tccggtcgaa gaggagcagg ttggcgctgt agagccagcc
     6121 ggagaccccg aagcgtagcg tctcgccgtc cacccgccgg tcgaaggcga gcgccgtctg
     6181 gcagagcggg caccaggtga ccaccacggg gcgtccgcca atctcgtcgt tcaccacctc
     6241 gtgcagggag agcagcttcg tcgggtatgc gcgggcgtca ccgtcgtgct cgacgccgac
     6301 gacgaggtcg ctcggcgaga cgagcgtcac ctcctcgggg gtttcgaaga ccgggtcgtc
     6361 gattgcaggt atcacgtcga tcccggcgcc gaccgacaac gcttcaggat cgaagtcccg
     6421 gagaaacgcc gcgttcacgc cgagatcccg gcggagatcc ggttcggcga caccgggttc
     6481 cgcctcgacc gtgtcgccc
//
LOCUS       DBNGNK010000368         5215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_349396,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000368 DBNGNK010000000
VERSION     DBNGNK010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_349396"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="AC150V_10965"
     CDS             <1..948
                     /locus_tag="AC150V_10965"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR3763479.1"
                     /translation="PDRVESRLVPEAGFELDTFEVSGIPRELSRSLLAASWKAARAPA
                     ACSEILERRRPDVVLGGGGFVAGPMVLAARRRGIPAALTEADAHFGLANRLAAPLARR
                     VFLAYPIPGRDEPKYRVTGRPIPETHMGATRAEGRSRFGLPSEGDVVAVFGALAGARS
                     LNEMAVSAWGEDGPAVLHICGERDYESLRGRVGREGYVLVSQTDHFGAALAAADLAVS
                     RAGGTVWELAAAGTPAVLVPYPHATADHQTLNARYFARGGGAVLVPDEEARQIPRLVR
                     ELLAEPERLQAMRTGMLALARPDAAEEIAEELIDLARAS"
     gene            932..2122
                     /locus_tag="AC150V_10970"
     CDS             932..2122
                     /locus_tag="AC150V_10970"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR3763480.1"
                     /translation="MPEPPDRPLAGRRVYFVGIGGSGLSAYANIARAWGAEVAGWDAR
                     ETIFMETLADVAIDVGGEPSPPSGWEVVVSTAHTGRAVGVSRSEFLGQLVEQQPSIVV
                     GGAHGKTTTAAMIAFVLRELGRDPAWIIGGVVPQLGGNAGAGSGWLVVEGDESDRSIG
                     ALRPRIAVVTNIELDHHAAYGSEAELRQFFDDWLASVDDVVLGWTLEPSDLQLAVPGE
                     HNRRNAAAALAALELAGVARSEAGPALSRFAGVGRRFELVGERGGVAVYDDYGHNPSE
                     LAAALRTARERTERRLIAVYQPHVYERTRHLHSELAGALALADAAVVTDVMGARDAPR
                     DGVTGKLVLDRLPPRVRAGWAPTLDDAVALTLRWARPGDVVVTLGVGEPWKIARAVVE
                     GLPA"
     gene            2119..3009
                     /gene="murB"
                     /locus_tag="AC150V_10975"
     CDS             2119..3009
                     /gene="murB"
                     /locus_tag="AC150V_10975"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR3763481.1"
                     /translation="MTIGLEKDVSLSRLTTIGTGGPARAFCRPATLAELEEALHWAAE
                     RDLPVVTVGLGSNLLCADDGVDALVLRLTGELAEVQARSNLLIAGGGATNAVCLHRAR
                     AAGLGGFEFACAIPGTAGGGVWMNAGAYGSDWAAILVRALVATAGGTGWLAPDELGLS
                     YRHSELRHGQVVARVEYRLNPAPPDEIRATVADLVARRKSTQPTNKRTFGSVFKNPPE
                     MGAGRMLEECGLKGHRVGGAAISPVHANFIENAGGATSEDCLALMVEARRRAREAFGV
                     ELEHEVVLLGGLVLPPLTAR"
     gene            3042..3902
                     /locus_tag="AC150V_10980"
     CDS             3042..3902
                     /locus_tag="AC150V_10980"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR3763482.1"
                     /translation="MGTGTRVRTAPEPRPRASGVVVAFPGLDTEERAALARLVPSGRV
                     LLLAFLGAAVIVGGYLLARESPLFAVQRIEVDGATPAVTRQVTRALGELEGTSLLKVD
                     PAELERVVEAIPTIREAAFDRAFPNSLRVVVVPERAVAVARQGESSWLISDRGRVMHK
                     VAKGKRGALPRIWVARSVRLEAGHVAGGALMTAITAVAPLDTSRLLGRVGMVSSSAED
                     LTLRMRSGLRIKLGEPSEVRLKLAIAAKILPLLAPEAVYLDVSVPERPVAGASLNSLV
                     DGESLTSTTP"
     gene            4008..5084
                     /gene="ftsZ"
                     /locus_tag="AC150V_10985"
     CDS             4008..5084
                     /gene="ftsZ"
                     /locus_tag="AC150V_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR3763483.1"
                     /translation="MNSQGGNYLAVIKVVGVGGGGTNAVTRMVESGVSGVEFVAVNTD
                     AQALLMTDADVKIHIGSKATRGLGAGADPAVGLAAAQESRDELKEALKGADMIFVTAG
                     EGGGTGTGAAPIVAELGRELEALTVGVVTRPFGFEGRRRAEQAEVGIQDLRERVDTLI
                     VIENDRLLQVVEKKTPVTEAFRMADDILRQGVQGLTDLITVPGLVNLDFADVRTIMRD
                     AGSALMGIGTASGENRATEAANEAVSSPLLETTLDGATGVLLNVTGGSDIGLFEVNEA
                     AEVVTGHADPNANVIFGAVIDEAMGEEVRVTVIATGFGGRQRRRRRAEPASAVAAGAR
                     SSSRENVTVETATDIDIPSFLRDE"
BASE COUNT          730 a         1794 c         1923 g          768 t
ORIGIN      
        1 cccgaccgcg tcgagtcgcg gctggttcct gaagcgggct tcgagctgga cacgttcgag
       61 gtctccggca tcccgcggga gctgtcgcgt tcgctgctcg cggcgagctg gaaggcggcc
      121 agggcaccgg cggcgtgcag cgagatcctc gagcgacgcc gaccggacgt cgtgctcggc
      181 ggcggcggct tcgtcgcggg cccgatggtg ctcgccgccc ggcggcgcgg catcccggcc
      241 gcgttgaccg aggccgacgc gcacttcggg ctcgccaatc gcctggcggc gccgctcgcg
      301 cgccgtgtct tcctcgccta cccgataccc ggccgcgacg agccgaagta ccgagtcacc
      361 gggcggccca ttcccgagac gcacatggga gcgacgcgcg ccgagggccg ctcgaggttc
      421 ggcctgccct ccgagggcga cgtcgtcgcc gtcttcggtg cgctggccgg cgcgagatcg
      481 ctcaacgaga tggcggtgag cgcatggggg gaggacggcc cggccgtcct ccacatctgc
      541 ggcgagcgcg attacgagtc gttgcgggga cgcgtcggac gcgaggggta cgtgctggtg
      601 tcccagacgg atcacttcgg ggccgcgctg gccgcagccg acctcgccgt ctcccgggcg
      661 ggcggcaccg tctgggagct cgccgcggcc ggcacgcccg ccgtgctcgt tccctatccg
      721 cacgccacgg ccgaccatca gacgctgaac gcgcgctatt tcgcgcgtgg gggcggcgcg
      781 gtgctcgttc cggacgagga agctcgccag atcccccgcc tcgtacgtga gctgctcgcc
      841 gaaccagagc gtctgcaggc catgcgcacc gggatgctcg cgctggcccg gcccgacgcg
      901 gcggaggaga tcgccgagga gctgatcgac cttgcccgag cctcctgacc ggccactcgc
      961 cgggcgccgc gtctacttcg tcgggatcgg cggctcggga ctctcggcgt acgcgaacat
     1021 cgctcgcgcg tggggggcgg aggtcgccgg ctgggacgct cgcgagacaa tcttcatgga
     1081 gaccctggcg gacgtcgcga tcgacgtcgg cggcgagccg agcccgccga gcgggtggga
     1141 ggtcgtggtc tcgacggctc acaccggacg tgcagtcgga gtgagccggt cggagttcct
     1201 gggccagctc gtggaacagc agccttcgat cgtcgtcggc ggcgcgcatg gcaagacgac
     1261 cacggccgcg atgatcgcct tcgtgcttcg cgagctgggg cgcgacccag cctggatcat
     1321 cggcggtgtc gtcccgcaac tgggaggcaa cgccggggcc ggctcgggct ggctggtagt
     1381 tgagggggac gaatcggatc gttcgatcgg tgcgcttcgg ccccgcatcg ccgtcgtcac
     1441 gaacatcgag ctcgaccacc atgcagcgta cgggtcggaa gccgagctgc ggcagttctt
     1501 cgacgactgg ctcgccagcg tcgacgacgt cgttctcggg tggacgctcg agccgagcga
     1561 cctccagctc gcggttcccg gcgagcacaa ccgccgtaac gccgcggcgg cgctcgctgc
     1621 cctggagctg gcgggggtcg cccgctccga agccgggccg gcgctctcgc gcttcgccgg
     1681 ggtgggaagg cgcttcgagc tcgtcgggga acgtggcggc gtcgccgtgt acgacgacta
     1741 cgggcacaac ccgagcgagc tggcggccgc actgcgaacg gcgcgcgagc gaaccgagcg
     1801 ccggttgatc gccgtctacc aaccgcacgt gtacgagcgc acccggcacc tgcacagcga
     1861 gctcgccggc gctctcgctc tcgccgatgc cgcggtggtc acggacgtga tgggcgcacg
     1921 cgatgcacct cgcgacggcg tcacgggcaa gctcgtgctc gaccggctgc cgccgcgcgt
     1981 gcgtgccggc tgggctccca cgctggacga tgccgtcgct ctcacgctgc gctgggcccg
     2041 gcccggcgat gtcgtcgtca cgctcggagt cggggagccc tggaagattg cccgcgctgt
     2101 cgtggagggc ttgcccgcgt gacgatcggc ctggaaaagg atgtgagcct ctcgcggctg
     2161 acgacaatcg gcacgggcgg ccccgcacgg gccttctgtc gtccggccac cttggcggag
     2221 ctcgaggagg cgctccactg ggccgccgag cgcgatctgc cggtcgtcac cgtcgggctc
     2281 ggctccaacc tcctctgcgc cgacgatggt gtcgacgccc tcgtcttgcg cctcaccggg
     2341 gagctggcag aggtgcaggc gcgatccaac ctgctgatcg ccggcggggg tgccacgaac
     2401 gcggtctgcc tgcaccgagc ccgcgccgcc gggctcggcg ggttcgagtt cgcctgcgcg
     2461 atcccgggca cggcgggagg cggcgtctgg atgaacgccg gcgcctacgg cagcgactgg
     2521 gccgcgatcc tcgtccgtgc ccttgtcgcg accgcaggcg gaacgggttg gctggcgccc
     2581 gacgagctcg gcctctcgta ccggcactcg gagcttcggc acggccaggt ggtcgcccgg
     2641 gtcgagtacc ggctcaaccc ggcgccgccg gacgagatcc gggccacggt cgccgacctc
     2701 gtcgcgcggc gaaagtcgac gcagccgacc aacaagcgca cgttcggctc cgtcttcaag
     2761 aacccgccgg agatgggcgc cggtcggatg ctcgaagagt gtgggctgaa aggccaccgc
     2821 gtcggcggag cggcgatctc gcccgtgcac gcgaacttca tcgagaacgc cggcggtgcg
     2881 accagcgagg actgccttgc gctgatggtg gaagcgagga ggcgcgctcg cgaggccttc
     2941 ggcgtggagc tcgagcacga ggtcgtgctc ctcggcggac tcgtcctgcc gccgctcacg
     3001 gccaggtagg agccgcgtcc cacgcggcga agccttctgc aatgggaacc ggcacgcgcg
     3061 tgcgaacggc gcccgagccg cgaccgcgtg cgtctggcgt cgtcgtcgcg tttccgggcc
     3121 tcgataccga ggagcgtgcg gcgctcgccc ggctcgttcc atccggacgc gttcttctgc
     3181 tcgccttcct cggcgcggcg gtgatcgtgg gcggctatct actcgcccgc gagtcgcctc
     3241 tgttcgcggt gcagcggatc gaggtcgacg gtgcgactcc cgccgttacc cggcaggtca
     3301 cccgcgcgct cggtgagctc gaaggtacga gcctgctgaa ggtcgacccc gcggagctcg
     3361 agcgagtcgt cgaggcgatc ccgacgatcc gcgaggcggc cttcgatcgc gcattcccga
     3421 actcgctccg cgtcgtcgtg gtgccggagc gcgccgtggc cgtcgcgcgg caaggcgaaa
     3481 gctcctggtt gatctccgac cgcggtcgcg tcatgcacaa ggtggcgaag ggcaagcggg
     3541 gggcgctgcc ccgtatctgg gtggcgcgaa gcgtccggct cgaggccggg cacgtcgcgg
     3601 gcggcgcgct gatgactgcg atcaccgcgg tcgcgccgct cgacacgtcc cgtctgctgg
     3661 gccgagtcgg aatggtgtcc tcgagcgcgg aggatctgac gctccggatg cgctcgggcc
     3721 ttcgcatcaa gctcggcgag ccctcggagg tccggctcaa gctcgccatc gccgccaaga
     3781 tcctgccgct gctggcgccc gaggccgtct acctcgacgt cagcgttccg gagcgtccgg
     3841 tagcgggagc aagtctcaac tctctagttg acggtgagag tttgacctcg accacccctt
     3901 gagatttccg ttgacagagc cgacaaggag tacgtaccct ggctccgaat gcgttacgaa
     3961 tgaaaccctt agctcgatga gagcttgacg gtgaggcagg tcgagagatg aacagccaag
     4021 gcggaaacta cctcgcggtg atcaaggtgg tcggcgtggg cgggggcggc acgaacgccg
     4081 tcacgcgcat ggtcgagtcg ggtgtgtccg gcgtcgagtt cgtcgcggtc aacaccgacg
     4141 cacaggcgct cctgatgacg gacgccgacg tcaagatcca tatcggctcg aaggcgacgc
     4201 ggggccttgg cgcgggggct gatcccgccg tcggcttggc cgcggcgcag gagagccgcg
     4261 acgagctgaa ggaggcgctg aagggcgccg acatgatctt cgtcacggcc ggcgagggtg
     4321 gcggcaccgg taccggcgcc gcgccgatcg tcgcggagct cggccgcgag ctcgaggctc
     4381 ttacggtagg agtcgtgacg cgcccgttcg gcttcgaagg gcgccggcgc gccgagcagg
     4441 ccgaggtcgg tatccaggac ctgcgcgagc gggtggacac gctgatcgtg atcgagaacg
     4501 accgcctgct gcaggtcgtc gagaagaaga ccccggtcac cgaggcgttc cgcatggccg
     4561 acgacatcct gcggcagggc gtccagggcc tcaccgacct gatcacggtg ccggggctcg
     4621 tcaatctcga cttcgccgac gtgcgcacga tcatgcgcga cgccggctcg gcactgatgg
     4681 gaatcggcac cgcgtccggc gagaaccgcg ccaccgaggc cgccaacgaa gccgtctcgt
     4741 cgccgctgct ggagacgacg ctcgacgggg cgaccggggt gctactgaac gtgaccggcg
     4801 gcagtgacat cgggctcttc gaggtgaacg aggccgcgga ggtcgtgacc gggcacgccg
     4861 acccgaacgc aaacgtcatc ttcggggcgg ttatcgacga ggcgatgggc gaagaggtcc
     4921 gcgtgaccgt catcgcgacg ggcttcggtg gtcggcagcg gcgacgtcgc cgcgcagagc
     4981 ctgccagcgc cgtcgcagcc ggggcgcgtt cgagctcacg cgagaacgtc actgtcgaga
     5041 cggccaccga catcgatatt ccgtcgttcc tgcgggacga gtagcctcgc atcgcccagc
     5101 gcggcgtgcg gccggccccc gggggtagag tcgcaagcgt gcagacgctg ctccggacgc
     5161 cgctccacga gcgccacgtc gcagccgggg cccgcctggt gccgttcgcc ggctg
//
LOCUS       DBNGNK010000369         5382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1067123,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000369 DBNGNK010000000
VERSION     DBNGNK010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1067123"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="AC150V_10990"
     CDS             <1..1140
                     /locus_tag="AC150V_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR3763484.1"
                     /translation="LAREIAADILGYGPIEPFLRDDTVTEVMVNGFDRIYVEREGIIE
                     KTDGAFLDEEHLYRIIDRIVSQVGRRVDEASPMVDARLPDGSRVNAIIPPLSLNGPSL
                     TIRKFARDPYKIEDLIGFGTLTPKSVQFLEACVRGKLNVLISGGTGTGKTTTLNALSA
                     YVPGAERIVTIEDAAELQLQQPHVVRLESRPANIEGSGEIKIRDLVRNALRMRPDRII
                     VGEVRGAETLDMLQAMNTGHEGSLTTIHANSPRDALNRLETLVLMSGLELPLHAIREQ
                     VSSALDLIVHLTRLVDGSRRVSHITEVLRMESEVITLQDVFRAHPVDERAAEVDPHAL
                     LRPLESTGLQPHFQAKLAANGVVLPPEFYEPELLEFELATNGSEQ"
     gene            1137..2126
                     /locus_tag="AC150V_10995"
     CDS             1137..2126
                     /locus_tag="AC150V_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3763485.1"
                     /translation="MREAIYDTAWGPVAVAIAVGCLMLVATVVAFARPRGAWLRDRLG
                     AYDGPAGGSAGGGGHSDSPRIRLETLYGATQRRFDHTRLWRAAQRLVDRSDVSFSTAE
                     ALFFSAGLGLAAAVVAAIGGVNGLLIAVALLAGILIVPTIMWFKARRRLNQFDEQLAD
                     TLLTMAGSLKVGHSFNQSVQAIVDKGTPPTSVEFDRLLSETKLGRPMDDSLASMSERI
                     GSSDLEFVLMSVRIQRQVGGSLAGLFETAAETVRERQQFRRKVSALTAMGRASAYVLL
                     ALPFVTGFVITLVNPGYLAPLFTTQKGQVLLLVGVGLMGLGAFVLSRIVNVKG"
     gene            2140..3033
                     /locus_tag="AC150V_11000"
     CDS             2140..3033
                     /locus_tag="AC150V_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR3763486.1"
                     /translation="MLFLAFVCLGTALVLAAGALTQPRREQQASLRRAAAYGGPQSGG
                     ATVSAWGRASERHTGTLAKLATRVDPRASVDRVGARLVSAGMARSVTPQGFLATKVVL
                     AGAGVIAGGLFGFSAGESLRALLLAVGLGVGGFYAPDVYVTLRTRSRRERIQRELPDA
                     LDILAVSVEAGLGFDAALAKVAEHLRGPLVEELTLVLHELRVGETRANALRKFAKRVD
                     LPDVTSFAQAIIHADQLGSPLGKVLRVQAAESRERRQIAAEERAMKAPVKMILPTFFF
                     IFPAMFIIILAPALFRINEAF"
     gene            complement(3096..3662)
                     /locus_tag="AC150V_11005"
     CDS             complement(3096..3662)
                     /locus_tag="AC150V_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR3763487.1"
                     /translation="MGEAVTSPDEATSPETDVVDDRLAVWHREIPSLDLATEGIIARI
                     QSIAKYLERSMNGTLSKFDLSFGEWKVLGSLRYSGEPYRLSPGDLSSCMNLSSGAMTN
                     RLDRMEEAGLIRRLPDPDDRRGLQIELTEKGWQLWQDTVEVQAEKEALIASALGEGEK
                     RELNDLLRRLLNSLSEHLRSEQGHHSKP"
     gene            3781..3999
                     /locus_tag="AC150V_11010"
     CDS             3781..3999
                     /locus_tag="AC150V_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763488.1"
                     /translation="MLVFGPDVAQLLATEHNERLRDAAGGRGPGRFRRRLGSSLIAAG
                     RKLHPDADRLRTVGPPDSAGPRFTSALS"
     gene            complement(4131..4628)
                     /locus_tag="AC150V_11015"
     CDS             complement(4131..4628)
                     /locus_tag="AC150V_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR3763489.1"
                     /translation="MQGRVLVVYATKYGSTEEVAEEIAGVLREDGIEVELSRMREVRD
                     IEGYGLVVLGAPLYMGRLLREGRSFVRKHRSALARVPHAVFALGPVGQDEGVEDESRG
                     QLERALAKAPELDPVAVALFWGVIRPERMRFPYNRMPAGDWRDWDEIRRFARSLPVRA
                     LEKVS"
     gene            complement(4768..>5382)
                     /locus_tag="AC150V_11020"
     CDS             complement(4768..>5382)
                     /locus_tag="AC150V_11020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3763490.1"
                     /translation="ISRWDRMKDMVGVMQSFAEHVDPALGAHLVLAGPVVVGVTDDPE
                     GAEVLGEVQAAWEGLSGELRSRVHLACIGMGDIAANARVVNALQRHSAVVVQKSIAEG
                     FGLTVSEALWKRRPVVASAVGGIVDQISDGVEGFLVESLDYAAAGRAIQGLLEDMELR
                     VRMGESGRRRVVNEFLADRHLEQYAELLAVLLGGRAVDPGRPAR"
BASE COUNT          859 a         1894 c         1737 g          892 t
ORIGIN      
        1 ctcgcccgcg agatcgcggc cgacatcctc ggctacggcc cgatcgagcc tttcctgcgt
       61 gacgacaccg ttacggaggt catggtcaac gggttcgacc gcatctacgt cgagcgcgag
      121 ggcatcatcg agaagacgga cggggcgttc ctcgacgagg aacacctcta ccgcatcatc
      181 gaccgcatcg tctcgcaggt gggacggcgc gtcgacgagg cgtcgccgat ggtcgatgca
      241 cgactgccgg acggcagccg tgtcaacgcg atcatcccgc ctctctcgct gaacggcccg
      301 agcctgacga tccgcaagtt cgcccgcgac ccctacaaga tcgaggacct gatcggcttc
      361 ggcacgctca cgcccaagtc ggttcagttc ctcgaggcgt gcgtgcgagg caagttgaac
      421 gtgctcatct ccggcggtac cggcaccggt aagacgacga ccctgaacgc gctttcggcg
      481 tacgttccgg gcgccgagcg catcgtcacc atcgaggacg cagcggagct tcagctccag
      541 cagcctcacg tggtgaggct cgagtcccgg ccggcgaaca tcgaaggctc gggtgagatc
      601 aagatccgcg acctcgtccg caacgcactg cgcatgcggc ccgaccggat catcgtcggt
      661 gaggtacgtg gcgccgagac cctggacatg ctccaggcga tgaacacggg ccacgagggc
      721 tcgctgacga cgatccacgc gaactcgcct cgggacgcgc tcaacaggct cgagacgctc
      781 gtgttgatgt ccggactcga gcttccgctg cacgcgattc gcgagcaggt gtcgagcgca
      841 ctcgacctca tcgttcacct gacacggctc gtcgacggct cgcgacgcgt ctcccacatc
      901 acggaggtgc tgcggatgga gtccgaggtg atcacactgc aggacgtctt ccgggctcac
      961 ccggttgacg agcgtgcagc ggaagtcgat cctcacgcac tgctccggcc gctcgagagc
     1021 actggcctgc agccccactt ccaggccaag ctcgcagcga acggcgtcgt gctaccgccc
     1081 gagttctacg agcccgagct cctcgagttc gagctcgcga cgaacgggag cgagcaatga
     1141 gagaagccat ctatgacact gcctggggcc cggttgcggt cgcgatcgcc gtcggctgcc
     1201 tgatgctggt ggcgacggtg gtggcattcg cgcggcctcg cggtgcctgg ctccgcgatc
     1261 ggctcggtgc atacgacggg cctgcgggcg gctcggccgg cggcggaggg cacagcgaca
     1321 gcccgcgcat ccggctcgag acgctgtacg gagctacgca gcgccgcttc gaccacaccc
     1381 ggctctggcg tgcggcccag cgactggtcg atcgctcgga cgtgagcttc tccacagccg
     1441 aggcgctctt cttctcggcg ggtctggggc tcgcggcggc ggtcgtcgca gcgatcggcg
     1501 gcgtgaatgg actgctgatc gcggtcgcgc tcctggccgg catcctgatc gttccgacga
     1561 tcatgtggtt caaagcgaga cggcggctca atcagttcga cgagcaactg gccgacacgc
     1621 tcctcaccat ggcgggctcg ctcaaggtcg ggcacagctt caaccagagc gtccaggcga
     1681 tcgtggacaa gggcacgccg ccgacgagcg tggaattcga tcgtctcctc tcggagacga
     1741 agctcgggcg gccgatggac gactcgctgg caagcatgtc ggagcgaatc gggtcttccg
     1801 acctggagtt cgtcctgatg tcggtgcgga tccagcgtca ggtcggcggc agcctggcag
     1861 gcctgttcga gacggccgcc gagaccgtgc gcgagcggca gcagttccgc cgcaaggtct
     1921 ctgcgctcac cgcgatgggt cgagcttccg cctacgtcct gctcgcactg ccgttcgtga
     1981 ccggcttcgt gatcactctc gtcaaccccg gttacctcgc accgctgttc acgacgcaga
     2041 agggccaagt cctgctgctc gtcggagtcg gcctgatggg actcggtgca ttcgtcctca
     2101 gtcgaatcgt gaacgtgaag gggtgatgga accgtgctga tgcttttcct cgcgttcgtc
     2161 tgcctcggta ccgccctggt gctcgccgcc ggcgcgctga cgcagcctcg gcgcgagcag
     2221 caggcgtcgc tccgccgggc agccgcgtac ggcggacccc agtcgggcgg cgcaaccgtc
     2281 tccgcctggg gccgggcgtc cgagcggcac acgggtaccc tcgccaagct ggccacccga
     2341 gtcgacccgc gtgcgagcgt cgaccgcgtc ggcgcccggc tcgtcagcgc gggaatggcc
     2401 cgctccgtca caccgcaggg ctttctcgcc acgaaggtcg ttctcgcagg cgccggcgtc
     2461 atcgccggag ggctgttcgg cttcagcgcc ggcgagtctc tccgcgcgct attgctggcg
     2521 gtcggcctcg gcgtgggagg gttctacgca cctgacgtct atgtcaccct gcggacgcgc
     2581 tcgcggcggg aacggattca gcgtgagctg ccggacgcac tcgacatcct cgccgtcagc
     2641 gtcgaggccg gcctcgggtt cgacgcagcg ctggcgaagg tcgccgagca cctgcgtggc
     2701 ccgctggtag aggagctgac actcgtcctc cacgagctca gggtcggcga gacgcgggcg
     2761 aacgcgctcc gcaagttcgc caagcgggtg gacctccccg acgtgacctc gttcgctcag
     2821 gcgatcatcc acgcagatca gttggggagc ccgctcggca aggttctccg cgtgcaggcg
     2881 gcggagtcgc gtgagcgtcg gcagatcgcg gccgaagagc gcgcgatgaa ggcgcccgtg
     2941 aagatgatcc tcccgacgtt cttcttcatc ttcccggcga tgttcatcat cattctcgcg
     3001 ccggctctgt tccggatcaa cgaggccttc tagcgaattc cgatgtgcgc ggggcggctc
     3061 cggccgccct cgcgcgccac tcggattcgc ctcgatcagg gcttcgagtg gtggccctgt
     3121 tcggagcgca gatgctccga cagcgagttg agcagccgcc tcaagaggtc gttcagctcc
     3181 ctcttctcgc cctcgcccag cgccgacgcg atcagcgcct ctttctcggc ctgcacctcg
     3241 accgtgtcct gccagagctg ccagcccttt tcggtgagct cgatctgcaa gccgcgtcga
     3301 tcgtcgggat ccggtagccg ccggatcagg cccgcctcct ccatccggtc gagtcggttg
     3361 gtcatcgcgc cgctcgagag gttcatgcat gacgagaggt cacctggaga gagccggtat
     3421 ggctcgcccg aatagcgaag cgagccgagc accttccatt ctccgaagct gagatcgaac
     3481 ttggagaggg tgccgttcat cgagcgctcc aggtacttcg cgatcgactg gatgcgggcg
     3541 atgatcccct cggtcgcgag gtcgagcgac gggatctccc ggtgccagac ggccagccgg
     3601 tcgtcgacga cgtcggtctc gggcgaagtg gcttcgtcgg gggaagtgac ggcttctccc
     3661 atcggtcaga ttctcgcgtt cgagttactt gacgtcaaga cagtcactcg tgtaagctcc
     3721 atttcaagta gttcggctta aagatacttg gacttgaatg gtagctgaga ggaggacgcc
     3781 atgctcgtat tcggcccgga cgtcgcccag ctgctcgcaa ccgagcacaa cgagcggctc
     3841 cgggacgcag ccgggggccg cggaccgggc aggttccggc gccggctcgg ctcctcactg
     3901 atcgcggccg gccgcaaact gcaccccgac gcagaccggc tgcgcaccgt gggcccgccc
     3961 gactccgcgg gcccgcggtt tacctccgcc ctctcctgac gctggactcg aagccctcta
     4021 cgcttccgcg cgctgcgcgt tacggtttcg taccaccggt cctcagaggt aggacgtggc
     4081 cctttcgtcc gagaccccct gtccggtgcg aactcccggg ctcgacccgg ctagctcacc
     4141 ttctccagtg cccgaactgg aagcgaacgg gcgaagcggc ggatctcgtc ccagtcgcgc
     4201 cagtctcccg ccggcatccg gttgtagggg aagcgcatcc gctccggccg gatcaccccc
     4261 cagaagagcg cgaccgcgac ggggtcgagc tccggcgctt tggcgagagc ccgctcgagc
     4321 tgcccgcgcg attcgtcctc cacgccctcg tcctgaccga cgggcccgag cgcgaacacc
     4381 gcgtgcggca cgcgggcgag cgcacttcgg tgcttcctga cgaaactccg accctcgcga
     4441 agaaggcggc ccatgtagag cggcgccccc agcacgacga gtccatagcc ttcgatgtcg
     4501 cgcacctctc gcatgcgcga gagctcgacc tcgatgccgt cctcgcgaag cacgccggcg
     4561 atctcctcgg cgacttcctc ggtcgagccg tacttcgtcg cgtagacgac aagcactcgg
     4621 ccctgcatcg gcacctccct ctttcgctct tcttctcgga gcagaccaga gcaggaacgg
     4681 acgcgcatcg gcgcgaacgc ggagcgcggg cgcggccaag ccgcagcgga gccggggggt
     4741 ctccgcgagc cgagacctcc ggaaccgtca gcgagctggg cggccgggat ccacagctcg
     4801 gcccccgagc agaaccgcca acagctccgc gtactgctcg agatggcgat ccgcgagaaa
     4861 ctcgtttacc acacggcgcc gaccgctctc tcccatgcgc actcgcaact ccatgtcttc
     4921 gagcagaccc tgaatcgccc gacccgccgc ggcgtagtcg agtgactcga cgagaaaacc
     4981 ttccacgccg tcgctgatct ggtcgacgat ccctcccacc gcagacgcaa ccaccggccg
     5041 acgcttccag agcgcctcgg agaccgtcag cccgaacccc tcggcgatgc tcttctgcac
     5101 gacgacggcg gaatgccgct gcaacgcgtt cacgacacgt gcgttcgccg cgatgtcgcc
     5161 catcccgata caggccagat gcacgcggga gcgaagctcg ccggagaggc cctcccatgc
     5221 ggcctgtacc tcacccagca cctccgcacc ctcgggatcg tccgtcaccc cgaccaccac
     5281 cgggccagcg aggacgagat gcgcccccag ggcggggtcg acgtgctcgg cgaaggactg
     5341 catcacgccg accatgtcct tcatccggtc ccatcgcgag at
//
LOCUS       DBNGNK010000370         5699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_635866,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000370 DBNGNK010000000
VERSION     DBNGNK010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_635866"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1022
                     /locus_tag="AC150V_11025"
     CDS             228..1022
                     /locus_tag="AC150V_11025"
                     /inference="COORDINATES: protein motif:HMM:NF025702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HIR3763491.1"
                     /translation="MKRRFLLLVVAAVTSLAVAGIANAAAVTVVHGVPGLTVDVYVNG
                     ELTLEGFEYETVTDPLDLPAGDYEIDIRAAGEPADSDPALSGSATVGEDTNASIVAHL
                     DEGGNPALTVYVNDTSTIQAGDARLTVRHTAAAPAVDVLANGNPLFTNLANPDEQSAD
                     VPADTYSVAVAATGTTAPVIGPLDLGLDAGTAYFAYAVGSLDENNLNVLVQTISGLGG
                     SPSAVPSGTVGLASTSSGFPLWGIALLSLAAVGVVTSGLALARSRR"
     gene            1067..1810
                     /locus_tag="AC150V_11030"
     CDS             1067..1810
                     /locus_tag="AC150V_11030"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HIR3763492.1"
                     /translation="MRRLGFLILTTSAVVLAVVPVWWVASRPDTSAGSLSGLELIDPV
                     AVVSTPSATPRRANRSEAERLAEAHRPKPRVVVPVRSARVEDLRGKVGPGPVSVRIPD
                     LEIDASVVPVGVERETGEMELPPDNATVAWYRHGASPGLEGSAVLAGHVDYGEGRAVF
                     FRLAELEPGARILIRYRGGALKAFRVVARRLVGKHELPARIFSTRGRPLLTLVTCGGD
                     YDAEDRSYLGNTLVFAVPLGAEPGGGSAQ"
     gene            1822..3078
                     /gene="kynU"
                     /locus_tag="AC150V_11035"
     CDS             1822..3078
                     /gene="kynU"
                     /locus_tag="AC150V_11035"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007515816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIR3763493.1"
                     /translation="MSHVAALAEERAARLETEDPVRSLRSEFAVPPWPDGAHPEIAYF
                     AGNSLGLMPVGARSAVEAELDEWARLGVEAWFDAKEPWLEYAGSLRAPLAAMAGASTE
                     EVAVVHSLTVNLHLLLASFYRPSEARYRVLVETGPFPSDAHAVRTHLALRGVPDDAIL
                     TSELDDLEATIDRHGVSIALVLLSGLSYLSGELLDIRAITAQARRAGAVVGWDLAHVI
                     GNVPLALHDCGVDFAAWCHYKYVNAGPGAPAGIFVHERHGRELSTPRLAGWWGTDPDV
                     RFLMAPEFVPRLGAEGWAVSTPPIIAHAPLRVSLDLFARIGLDALRERSIRLTGYLEE
                     LLDVVAHSSRIALITPRAVERRGAQLSVSVRSARELSERLRREHGVVCDFREPDVLRL
                     APAPLYNTFEDCRRAAVALLDVVPAR"
     gene            complement(3082..3360)
                     /locus_tag="AC150V_11040"
     CDS             complement(3082..3360)
                     /locus_tag="AC150V_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763494.1"
                     /translation="MPQRPPPVATTQRRPPVALPSTVVTTSNAPGAVHWAIIAALGLG
                     LGLLVAAALPRRFIASMPAPFRDRAQLVAGVTGLTMAVGAFVALLTAA"
     gene            complement(4035..4808)
                     /locus_tag="AC150V_11045"
     CDS             complement(4035..4808)
                     /locus_tag="AC150V_11045"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763495.1"
                     /translation="MSRSISTAPAALYLHELRYQQLTFWRSRESAIFVFLFPLLIYAL
                     LGAVYDETIDGEPAADVLLAGLLGYGCANTAFAGLAIQLVIRRENGLLKRIRATPLPG
                     PTYVAALLSSVLLTFALQTVALILLGRLLYDASWPERPGSLVLAILLGVAAFAAMGVG
                     VAGLIRSAEGASAVVNVILLPMAFLSGAFGPTRDLPDWLQAIADVLPLRYFLDIVRGA
                     YIEGAGFFADPSAILIVAIWGVAGLLVAWRRFSWEPRER"
     gene            complement(4805..5659)
                     /locus_tag="AC150V_11050"
     CDS             complement(4805..5659)
                     /locus_tag="AC150V_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005310713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763496.1"
                     /translation="MPPAIAVHDLRKRYGELEALAGVSFEVEPGEVFGLLGPNGAGKT
                     TTVEILEGYRKRDGGEARVLGFDPGRAQRALRERIGVVLQQSELWPQLSVREVVQVFA
                     GYYRGRRDVDEVVALVGLDEKRSARVKTLSGGQKRRLDLAVALVGDPDVVFLDEPTTG
                     FDPAARRNAWDMIRSLRALGKTILLTTHYLDEAQQLADRVAVIVAGRIVELGTPSELI
                     GSTRKTEIRYRLDGQDVVIETEEPTATLNRVTAEALARGGELDHLEVRRPSLEDVYLR
                     LIDENGEE"
BASE COUNT          857 a         1942 c         1967 g          933 t
ORIGIN      
        1 ccagccctgc ggctccatga ccacgccgtg ggcgagctcg atagcgatcg acgtcatccc
       61 tcatacggat gatcggatcg ctgctttcgc gcgtccccct tcggagggga gcgctcgtgc
      121 cctggcatcc agtcggcgag gggatcaagt gccagataac tggacaaaat ttgtacatgt
      181 tgtgtacgat tcgttctatt cggtcaacga tctctaggag tacagcaatg aagcgcaggt
      241 ttcttcttct cgtcgtcgcc gctgtaacgt ctctcgcggt cgccggcatc gcgaacgccg
      301 ccgccgtcac cgtcgtccat ggagtgccag gtttgacggt cgacgtgtat gtcaacggtg
      361 aactcacgct cgagggcttc gaatacgaga ccgtcaccga cccgctcgac ctacccgccg
      421 gcgactacga gatcgacatt cgtgcggctg gcgagccggc ggactcggac ccagcccttt
      481 ccggcagtgc gacggtcggt gaagacacca acgcgtcgat cgtggcgcac ctcgacgagg
      541 gtggcaaccc ggccctcacc gtctacgtga acgacacgtc gaccatccag gcgggcgacg
      601 cccggttgac ggtcaggcat acagctgccg ctcccgccgt cgacgtgctt gcgaacggca
      661 atccgctctt cacgaacctc gcgaatcctg acgagcagtc ggccgacgtt cccgccgaca
      721 cctactcggt ggccgtcgcc gcgacaggga cgaccgcacc cgtcatcggc ccgctcgacc
      781 tcggcctcga cgcgggcacc gcctacttcg cgtacgcggt cggctcgctc gatgagaaca
      841 atctcaacgt cttggtgcag acgatcagcg gcctcggcgg gtcgccctcg gccgttccct
      901 cgggcactgt gggcctcgca tccacctcca gcgggttccc gctctggggc atcgcgctgc
      961 tgtcgctcgc ggccgtcggt gtcgtgacct ctggtctcgc cctcgcccgc tcccgacgct
     1021 gaccgtacga tcaaacggcg ggggcacatg cccccgccgc tcgccgatga gacgtctcgg
     1081 attcctgatc ctcactacaa gtgctgtcgt gctggcggtc gtccccgtct ggtgggtcgc
     1141 ctcccgaccc gacacctcgg ccggttcgct ctcgggcctc gagctgattg accctgtcgc
     1201 ggtcgtgtct acgccgtcgg caacgccgcg ccgggcgaac cgctccgaag cggagcgcct
     1261 cgcggaggcg catcgcccga agcctcgagt cgtggttccg gttcggagtg cccgtgtcga
     1321 ggacctgcgg ggcaaggtcg gcccgggtcc cgtgtccgtt cgcatcccgg acctcgagat
     1381 cgatgcgtcg gtcgtgccgg tcggcgtcga gcgcgagacc ggggagatgg agctgccgcc
     1441 cgacaacgcg acggtggcgt ggtaccggca cggtgcatca ccgggcctgg agggctccgc
     1501 cgtgctggca ggccacgtcg actacggcga gggtcgggca gtcttcttcc ggctggcgga
     1561 gctcgagccg ggagcgcgca tcctgattcg ctaccgaggc ggtgccttga aggcatttcg
     1621 cgtggtcgca cgccgcctcg ttggcaaaca cgagctgcct gcgcggatct tctcgacgcg
     1681 aggacgccca ctccttacgc tggtcacctg cggaggcgac tacgacgccg aagaccgcag
     1741 ctacctgggc aatacgctcg tgttcgcggt tccgctgggc gccgaacccg gcggcggaag
     1801 cgctcagtag gctcccgagt catgtctcac gttgccgccc tcgccgaaga acgtgcggct
     1861 cgtctcgaga cggaggatcc ggtccgctcg cttcgctcgg agttcgcggt tccgccctgg
     1921 ccggacggtg cccatcccga gatagcgtat ttcgcgggca actcgcttgg actcatgccg
     1981 gtcggcgctc gctcggcagt cgaggcggag ctcgacgagt gggcgagact cggcgtcgag
     2041 gcttggttcg acgcgaagga accctggctc gagtacgccg gctcgctgcg cgcgccgctt
     2101 gcggcaatgg cgggtgcctc gaccgaggag gtcgccgtcg tccactcgct gacggtcaat
     2161 ctgcacctgc tgctcgcctc gttctaccgg ccctccgagg cgcggtaccg cgtcctcgtc
     2221 gagactggtc ctttcccctc cgacgcgcac gccgtccgga cccatctcgc cttgcgaggc
     2281 gtgccggacg acgcgattct cacctcggag ctcgacgacc tcgaggcgac gatcgatcgg
     2341 cacggggtct cgatcgcgct cgtgctcctt tccggtctca gctacctctc cggcgagctc
     2401 ctcgacatcc gcgctataac ggcgcaggcg cgacgggccg gagcggtcgt cggctgggat
     2461 ctggcacacg taatcgggaa cgtcccgctc gcgctccacg actgcggtgt cgacttcgcg
     2521 gcgtggtgcc actacaagta tgtgaacgcg ggtcccggag cacccgcagg aatcttcgtg
     2581 cacgagcggc acggccgtga gctctcgacg ccccgcctcg ccgggtggtg gggaaccgac
     2641 cctgacgttc ggttcttgat ggccccggag ttcgtaccgc gtttgggcgc ggaaggctgg
     2701 gccgtctcga cgcctccgat catcgcgcat gcgccgctcc gagtctcgct cgatctgttc
     2761 gctcgaatcg ggctcgacgc gcttcgcgag cgctcgatcc ggctgaccgg ctacctggag
     2821 gagctgctcg acgtcgttgc tcacagcagt cggatcgcgc tgatcactcc gcgtgccgtc
     2881 gaacgccgag gcgcgcagct gtccgtgtcg gtgcgctcgg cacgtgagct gagcgagcgg
     2941 ctacgtcgag agcacggtgt cgtctgcgat ttccgtgagc cggacgtgct gcggctcgct
     3001 ccagctcccc tctacaacac cttcgaagac tgccggcgag ccgccgtggc attactcgac
     3061 gtcgtgccgg cgcgctgacg gtcaggcggc cgtgagcagg gcgacgaaag cgccgacggc
     3121 catcgtgagg ccggtgactc ctgccacgag ctgagcccgg tcgcggaacg gagcaggcat
     3181 cgaggcgatg aagcgtcgcg gcagagccgc tgcgacgagc aggcctagtc ccaggccgag
     3241 cgcggcgatg atcgcccagt gcacggcgcc cggtgcgttc gaggtggtga cgacggtcga
     3301 cggcagggcg accggcgggc ggcgctgagt cgtggcgacc ggcggcggcc gctgaggcac
     3361 cggaacgatt ccgactcctg tcgggccggc ccttccttcg cgggttgccg gcggcaggtt
     3421 cttcccagca atggcttttg ccttcgcggc tttcgctttg gcggctttcg ccttcgcggg
     3481 ttgctgagcc ccttttggcc tggccggcgt gggcggcggc ggggtcgcgg ctggcggcgc
     3541 gggcgggggt gggggtgggg gtggcggaga gccgggggcg ggcgccggcg accggggtgg
     3601 cggtgccggc ggcggagccg gtggcgaggc gggcgttgac ggagccccga ccggcgcaag
     3661 attcggcacc acggagccga ccggctgcgg cggcgccgga ggctgcgccg gaggctgcgg
     3721 cttcggtgcg ggtgaagatg gagccggcgg cggatcggcc accggaggca gcggggtttc
     3781 gatcgtctcc ggaggtggcg tcggagctgg cggaacggga tcggccaccg gcggaggtac
     3841 agtgcccgag cctcctgtgc ccacgggcgt cggggtcggt gtcgtcgtcg ctgcggtcgg
     3901 cacggctacc agcgccaggc cggcgatgag aaacacgatt gcccgccggg ggctcacgtg
     3961 ccggcctatg accaaccccc tcggttcgcg tgacgttctt caaccatcct atcgccggcg
     4021 tcgtgccgac gaagtcagcg ttctcgcggc tcccagctga agcggcgcca cgcgacgaga
     4081 agacctgcga cgccccagat cgcgacgatc aggatcgcgc tcggatcggc gaagaaaccg
     4141 gcgccctcga tgtacgcccc ccggacgatg tcgaggaagt accggagggg cagaacgtcg
     4201 gcgatcgcct ggagccagtc gggcagatca cgcgtcggcc cgaaagcgcc tgagagaaag
     4261 gccatcggca gcaagatcac gttgacgacc gcggaagcgc cctcggccga gcggatcagt
     4321 ccggcaacgc ccacacccat ggccgcaaac gcggccacgc ccagcaggat cgcgagcacg
     4381 agggatcccg gccgctcagg ccacgaagcg tcgtagagca ggcgcccgag caggatcagc
     4441 gcgaccgtct ggagtgcaaa ggtcagaagc accgagctca gaagcgccgc gacgtacgtc
     4501 ggtcccggca ggggagtcgc gcggatccgt ttcaggagcc cgttctcccg ccggatcacg
     4561 agctgaattg ccagacctgc gaaggcagtg ttggcgcagc catagcccag caggcccgcc
     4621 agcagcacat cagcggccgg ttctccgtcg atcgtctcgt cgtagacggc tccgagcagc
     4681 gcgtagatga gcaggggaaa gaggaagacg aagatggccg actcgcgact gcgccagaac
     4741 gtcagctgtt ggtagcgcag ctcgtgcagg tacagggcgg cgggagcagt ggatatcgag
     4801 cgactcattc ctcgccgttc tcgtcgatga gccgcaggta cacgtcctcg agtgacggtc
     4861 ggcgcacctc gaggtgatcg agctccccgc cgcgagccag cgcctcggcc gtgacccgat
     4921 tcagcgtcgc cgtcggctct tcggtctcga tcacgacatc ctggccgtcg agccggtacc
     4981 ggatctcggt cttccgcgtc gagccgatga gctcggacgg cgtgcccagt tcgacgatgc
     5041 gaccggcgac aatcacggcg acacggtcgg cgagctgctg tgcttcgtcg aggtaatgcg
     5101 tcgtcagaag gatcgtcttg ccgagggctc gcagcgatcg gatcatgtcc caggcgttgc
     5161 gtcgagcggc cggatcgaag cccgtcgtag gctcgtccag aaagacgacg tcgggatcgc
     5221 ctacgagggc cacggcgaga tcgagccggc gcttctgccc cccggagaga gtcttcaccc
     5281 gcgccgatcg cttctcgtcg agaccgacga gcgcgacgac ctcgtcgaca tcccgacgtc
     5341 cccggtagta gcctgcaaag acctgcacga cctctcgcac cgagagttgc ggccagagct
     5401 ccgactgctg gagaacgact ccgatccgct cccgaagggc ccgttgggcg cgacccggat
     5461 cgaagccgag gacgcgtgcc tcgccaccgt cccgcttgcg gtagccctcg aggatctcga
     5521 ccgtcgtggt cttgcccgcg ccgttcggtc cgaggaggcc aaagacctcc cccggctcga
     5581 cttcgaacga gacgcccgcg agcgcctcga gctctccgta acgcttgcgg agatcgtgga
     5641 cggcgatcgc gggcggcatg gccgccgcga tgctagacgc tgtcgcttcg tctcggcag
//
LOCUS       DBNGNK010000371         3681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1927562,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000371 DBNGNK010000000
VERSION     DBNGNK010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1927562"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..1327)
                     /locus_tag="AC150V_11055"
     CDS             complement(77..1327)
                     /locus_tag="AC150V_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR3763497.1"
                     /translation="MTTHHPGRPRRYRRREFLQRGAMGALGLSLAPSLLAACGGSDGG
                     ALEVDGIPLARPDNPLTLPIFDDNPPVESGLEPEAGPLRLYNWVDYVWPKTLKDFQKE
                     FGVKVEMTTFYTMDEAIAKIGSGTANFDVFWPTTDRISRLVYGKTLQPLNHDYLPNMS
                     NVWPAALDPFYDQGSQYTLPYTVFTTGIGYRVDDTGAGPQDLENPYDFYWESPKGKTF
                     LLDDSREAISMALLRNGNLDLNTEDPDQIEQAKEDLLSLIGAVNVKVSVEDYTKLPEG
                     AAGTHQAWSGSMIAAQWYVPEGDSVEVLRYWFPEDGVGPIGIDEMAIMKNAKSPVLAH
                     AFINYMLDNDVAYDNFAEFNGYQPPLVALDVDKLVTDEVVPANLDTSIVRESDFESGL
                     QELELSPDGQVLWQNAWAEFKAGL"
     gene            1481..1882
                     /locus_tag="AC150V_11060"
     CDS             1481..1882
                     /locus_tag="AC150V_11060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR3763498.1"
                     /translation="MQHEKRIEIRWRDVDAYKHVNNAVYATYLEECRDEWLEAVLGAV
                     DDLWDFVLARVAIDFRRELVQDDDAVVVSCTLERIGTSSVSTREEIRTEEGELAAEAA
                     AVLVARDPATGSSRPLREDELAALRAAAPGI"
     gene            1964..3130
                     /locus_tag="AC150V_11065"
     CDS             1964..3130
                     /locus_tag="AC150V_11065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3763499.1"
                     /translation="MIVPELPAEIRELKERVGRFVEAEVYPLESRIAEAGAIDHAEVD
                     VIRHKAREQGFAMLNMPVEHGGRGLSMLGQVALEEESGKATNGLGFAVVDRGPRELLE
                     IATPEQIRRYIEPVVHGEFREAWAVTEPGAGSDVGNMSTTAVRDGDSWVLNGEKWFVT
                     SEGEPGYYLVLAVADGEQALFIVEPGVPGFEVKRTPRFLHDPYLDHHSELLFRDCRVP
                     DGQRIPEGGGEGARDWFMVERLFIAARCCGAAERLLDDASAWAQEREAFGVPLSQHQG
                     VSFQLADSLTELMAARLLTYHAAYAFDTLADRRIVHGKVAMAKLYASEMAGRVADRAL
                     QVFGGRGYMVENPAARFYRELRVDRIWEGTSEIQRLIISRGLFKRGAAAYLGWD"
     gene            complement(3164..>3681)
                     /locus_tag="AC150V_11070"
     CDS             complement(3164..>3681)
                     /locus_tag="AC150V_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR3763500.1"
                     /translation="KALGPLHKSERGGVELQLEDAHALAAAFTRMAGRLHPRAYSVEH
                     MADTAEGIELIVGCKRDPRFGPIVLVGLGGVYAELIRDVAVALAPSGADEQAARLRSL
                     RGSPLLLGTRSRPALALEAAAEAAAALSRLSAERPLLAEVEVNPLLVTRDGVLGLDAR
                     AVVRVPSAHRA"
BASE COUNT          550 a         1203 c         1321 g          607 t
ORIGIN      
        1 cgagcagcca gacgatgccg ggaacggcgc tgaccggcca aaaccagcgc gggtagcgca
       61 cgtcttcgtt cgggggctag aggccggcct tgaactcggc ccaggcgttc tgccagagca
      121 cctgtccgtc cgggctcagc tccagctcct ggaggccgct ctcgaagtcc gactcgcgca
      181 cgatcgaggt gtcgaggttg gcgggcacga cctcgtcggt cacgagcttg tccacgtcga
      241 gagccacgag cggcggctgg tagccgttga actcggcgaa gttgtcgtac gcgacgtcgt
      301 tgtcgagcat gtagttgatg aacgcgtgcg cgagcaccgg gctcttggcg ttcttcatga
      361 tcgccatctc gtcgatcccg atcggcccga cgccgtcctc ggggaaccag tagcggagca
      421 cttcgacgct gtcgccttcg ggcacgtacc actgggccgc gatcatgctc ccggaccagg
      481 cctggtgggt tcccgccgct ccctcgggga gcttggtgta gtcctcgacc gacaccttca
      541 cgttcactgc tccgatcaag ctgagcaggt cttccttcgc ctgctcgatc tggtcggggt
      601 cctcggtgtt gagatcgagg ttcccgttgc ggagcaacgc catgctgatc gcctcgcgcg
      661 agtcgtcgag caggaaggtc ttgcctttcg ggctctccca gtagaagtcg tacgggttct
      721 cgaggtcctg cgggccggcg ccggtgtcgt cgacccgata gccgatgccg gtcgtgaaca
      781 ccgtgtaggg cagggtgtac tgagagccct ggtcgtagaa gggatccagc gccgccggcc
      841 agacgttgct catgttcggc aggtagtcgt ggttgagggg ctgcagcgtc ttgccgtaga
      901 cgaggcggga gatccggtcg gtcgtgggcc agaagacatc gaagttcgcg gtgcccgagc
      961 cgatcttcgc gatcgcctcg tccatcgtgt agaaggtcgt catctcgacc ttgacgccga
     1021 actccttctg gaagtccttc agcgtcttcg gccagacgta gtcgacccag ttgtagaggc
     1081 gcagcggacc ggcctcgggc tcgagcccgg actcgaccgg cgggttgtcg tcgaagatcg
     1141 ggagagtgag cgggttgtcg gggcgggcga gcgggatgcc gtcgacctcg agcgcgccgc
     1201 cgtcggagcc gccgcaggcg gccagcaggg agggagcgag cgagagcccc agggccccca
     1261 tcgcaccacg ttgcaggaac tcgcgccgcc tgtaccgtcg ggggcgaccc gggtgatgcg
     1321 tcgtcatcgg ccgatcctcc tcgtccgttc agcccggtaa gcctggtcac tgaagcagag
     1381 cgtcaccggt tggtcaactt ctccgttctc tctgagacgg cgtggcgcag atgcggatag
     1441 ctgcacggac gttgagaacg ccggggtagg atcgcgcccc gtgcagcacg agaagcgaat
     1501 cgagattcgc tggcgtgacg tcgacgccta caaacacgtc aacaacgccg tctacgccac
     1561 ctatctcgag gagtgccgcg acgagtggct ggaggcggtc ctgggtgcgg tcgacgacct
     1621 gtgggacttc gtactcgccc gggtggcgat cgatttccga cgcgagctcg tccaggacga
     1681 cgacgccgtc gtcgtcagct gcacactgga gcgaatcggc acctcgagcg tctcgacgag
     1741 ggaggagatc cgtacggagg agggcgagct ggcggccgag gctgccgccg ttctcgtcgc
     1801 gcgcgatccc gcaacgggca gctcgcggcc gctgcgggag gacgagctgg cggcgctccg
     1861 ggccgcggct ccgggcatct agggactcga gcccgcgccg tcgtctagat tcccgccgat
     1921 ccgggcgcgt cggcgcccgc ccatcccgcc aggaggttcc gtcgtgatcg tccccgagct
     1981 ccccgctgag atccgtgagc tgaaggagcg cgtcggccgc ttcgtcgaag cggaggtcta
     2041 tccactcgag tcgcgcatcg ccgaggcggg tgcgatcgac cacgccgagg tcgacgtgat
     2101 tcgacacaag gcgcgcgagc aaggtttcgc catgctcaac atgcccgtcg agcacggcgg
     2161 ccgtgggctc tcgatgctcg ggcaggttgc tctcgaggag gagtcgggca aggccaccaa
     2221 cggactcggt ttcgcggtcg tcgaccgcgg gccgcgcgag ctgctcgaga tcgcgacgcc
     2281 cgagcagatc cgccggtaca tcgagccggt cgtgcacggc gagtttcggg aggcgtgggc
     2341 cgtcaccgag cccggtgcgg gctcggacgt gggcaacatg tccacgacgg ccgtccgcga
     2401 cggcgactcc tgggtgctga acggcgagaa gtggttcgtc acgagcgagg gggagcccgg
     2461 ctactacctg gtgcttgccg tcgcagacgg cgagcaggcg cttttcatcg tcgagcccgg
     2521 ggtacccggc ttcgaggtca agcgaacgcc gcgcttcctg cacgacccgt acctcgacca
     2581 ccactcggag cttctcttcc gcgactgccg cgtaccggac gggcagcgca ttccggaggg
     2641 cggcggcgag ggggcgcggg actggttcat ggtcgagcgg cttttcatcg cggcgcgttg
     2701 ctgcggtgcg gccgagcgcc tgctcgacga cgcgagcgcc tgggcgcagg agcgcgaagc
     2761 gttcggcgtg cccctctcac agcatcaggg tgtttcgttc cagctcgcgg actcgctcac
     2821 ggagctgatg gctgcccgcc tgctcacgta ccacgccgca tacgctttcg acaccctcgc
     2881 cgaccggcgg atcgtccacg gcaaggtggc gatggcaaag ctctacgcct ccgagatggc
     2941 gggtcgtgtg gccgaccggg cgctccaggt gttcggtggc cgcggctaca tggtcgagaa
     3001 tccggcggcg cgcttctacc gcgaacttcg cgtcgatcgc atctgggagg ggacgagcga
     3061 gattcagcgg ctgatcatct cgcgcgggct cttcaagcgt ggggccgccg catatctcgg
     3121 ctgggactaa tgccgtcgcg ccggtcggcg cggaacgtcc gactcacgcc cggtgcgcgg
     3181 agggcacgcg gacgactgcg cgcgcgtcga gcccgaggac gccgtcgcgg gtgacgagga
     3241 gggggttgac ttcgacctcg gcgagtaggg ggcgctcggc gctgaggcgg gagagcgcgg
     3301 cggcggcctc tgccgcggcc tcgagcgcca gtgccggccg gcttctcgtg ccgagtagga
     3361 gcggcgagcc gcggagcgag cggagccgcg cggcttgctc gtcagctccc gacggggcga
     3421 gcgcgaccgc gacgtcgcgg atcagctcgg cgtagacgcc gcccaggccg acgaggacga
     3481 tcggcccgaa gcgagggtca cgcttgcagc cgacgatcag ctcgatcccc tccgctgtgt
     3541 ccgccatgtg ctcgacggag taggcccgcg ggtgcagacg gccagccatt cgcgtgaacg
     3601 ccgcggcgag cgcatgcgcg tcttccagct gcagctcgac tccaccccgc tccgacttgt
     3661 gcagcggacc gagcgccttg a
//
LOCUS       DBNGNK010000372         6075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1709327,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000372 DBNGNK010000000
VERSION     DBNGNK010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1709327"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="AC150V_11075"
     CDS             complement(<1..1097)
                     /locus_tag="AC150V_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR3763501.1"
                     /translation="MDAWTLAVIAGLVLVLASLSRWIEKSILTGPIVFLSAGLVFGNE
                     GLGWINLSGGDGSVRLLAEVTLALVLFLDASRINMRVLRRELGVPSRLLGIGLPLTLI
                     LGTLVALMFEEITFGEAFVLAVVLAPTDAALGLDVVTDRRLPSRVQQSLNVESGLNDG
                     LCVPLLFIAIAVAETEEGALAGSDAARIVIEEIGYGTLAGVAAGVVGAGALGFARRHR
                     LITQEWAQVIPLAAATLAFGIAAPIGGSGFIAAFVGGLVYGVLRTETRAEERHFLDVT
                     GHTLTAVTFVIFGAIIAGPALAGLDWRAAVYAVASLTVVRMLPVGLSLIGTGFRKQSV
                     AFTAWFGPRGLASLVFAVIVLEEANLPHLDLL"
     gene            complement(1153..2745)
                     /locus_tag="AC150V_11080"
     CDS             complement(1153..2745)
                     /locus_tag="AC150V_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR3763502.1"
                     /translation="MLEIILTREDANTDVAILIEWLADDGAEVNKGEPVCVVETTKAS
                     LEFEAPGDGTLVHLYDAGAEVELGRTIALVAESADELEQARSSRTAAPAEKAETAAPT
                     QRKATKKAVELAEKHGIALDEIDKQGFITAEDVEALVAAQAAEDGGPDPVFAGISTQN
                     VSLPASLRGEDEGAGVVDPALLELIASDPNRFRSLSSDEKVATYRQHGARIGEGVVLE
                     DGALVVAPRIVLGDRVRIGQGGSIVCAEVVAIGEVSHFGPGLDLRCRRAHLGAGIHCS
                     GSVQIGGGGHHDPWATLTIGDHTFLGSEAFVNVCRPVLIGSEVFLTMRSMIVTHNIGH
                     SVLEGFENRFAGVVLEDRSQVGLGTVVYAGCRIGREAIVASSSYVVSDIPEGSLAIGV
                     PAKAVGHAKRTLSRQQQTEASHRMLDELQELISLRGHHVEPLPEGRGFAVTGDTGRSH
                     VLLVDRLRASDSIPVAEAETVVLTLEYSGDQAPDGVAVIDLLGRQLHGGGGVVLDSVR
                     EFCRKRGIKLSPGPWRYPGGLI"
     gene            complement(2798..3820)
                     /locus_tag="AC150V_11085"
     CDS             complement(2798..3820)
                     /locus_tag="AC150V_11085"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR3763503.1"
                     /translation="MVQKRCVHVINEALHELLETRDNVYVLGEDVLDPYGGAFKVTQG
                     LSDRFPERVLTTPISEASLFGVAAGMAMRGKRPILEIMFGDFVALGYDQIVNGISKFR
                     EMFDEQVTVPLVVRTPMGGRRGYGPTHSQSLEKLLIGVPGIVVVAASECHDRGGLLAA
                     ATDDDRPVFLIENKLMYGRPTRRPVDGFVDELRCVESDGPYPALSFSGNDFTDSAATI
                     VTYGGMLPIVLDAVTELILEHEVFCEVVALSRLLPPDLDPVLESASRSGAVITAEEGG
                     LTGGYGAELAARIQEHAWDDLRAPVRRIAARDGIIPAAPGLEAKALPGVNDVVDAVAA
                     LAGVRS"
     gene            complement(3820..4752)
                     /locus_tag="AC150V_11090"
     CDS             complement(3820..4752)
                     /locus_tag="AC150V_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014690091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR3763504.1"
                     /translation="MATDTATDVAAARTGLLEQLYERMYFIRRFEETLLEMFAEGKLV
                     GTTHTYIGQEANAAGVIAHLEPERDVVFSNHRCHGHYLAFTGDAFGLLSEVMGKATGT
                     CGGKGGSQHLCKGNFYSNGVQGSIVPVSTGIALAEKEKRSGAVTTVFLGDGTLGQGAV
                     YECLNMASLWQLPLLFVVENNEYAQTTPRRLAFAGSIPERAAAFGVPTAELDTTDVLE
                     IHETAGRAVERVRTTGSPFFLVLNTYRFSPHSKGDDFRDPGEIDERRTRDPLRVSGAK
                     LGDAERIAIEESCEQRLAAIIAEAEAAPDAGGDL"
     gene            complement(4762..5952)
                     /locus_tag="AC150V_11095"
     CDS             complement(4762..5952)
                     /locus_tag="AC150V_11095"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase family protein"
                     /protein_id="HIR3763505.1"
                     /translation="MNLVVYVSDALRTDHVGCYGARHVNTKTIDELAAGGARFDQAIS
                     VAPWTCPSMTSMTTGIEPHHHGVLHWDRELDPGTPTLFTTAAAAGYQVASFVFDENYL
                     FKGWPEANVLGTTETLDGVIAWLRESRQEPFLLFVHSWATHMPYDILHTERKEWLAAK
                     EEIITGIQSNTADGLLTLHEGYARAVERQSEVLFASFLEELETLGLREQTVLAFTSDH
                     GESWGERFTEKDDVKGIYHMHGATLYEEVVQVPLILSAPGRVDPRTVWSQVRSTDLMP
                     TLLELTGIPIPELDGASLLPLLDGSEGGDRPAVISGTDMGALSQLAVRMPPWKLIHHV
                     ESGREEAYKLDVDPRETTSRPGDVPPELRERLYAELEGAERHELSEDEEATVAARLAD
                     LGYL"
BASE COUNT          935 a         2092 c         2103 g          945 t
ORIGIN      
        1 agcaggtcca ggtgcggcag gtttgcctcc tcgagcacga tgacggcgaa cacgagcgaa
       61 gcgaggccac gcggcccgaa ccacgccgtg aaggcgacgg actgcttgcg aaagcccgtg
      121 ccgatcaatg acaggccaac gggaagcatg cgaacgacgg tcaggctggc caccgcgtag
      181 acggccgcgc gccagtcgag cccggccagc gccggaccgg cgatgatcgc tccgaagatg
      241 acgaacgtca ccgccgtgag cgtgtggcct gtgacgtcga ggaagtggcg ttcctcggcg
      301 cgggtctcgg tgcggagaac gccgtagacg aggccaccga cgaaggcggc gatgaagccg
      361 ctgccgccga tcggtgcggc gatgccgaag gcgagggtgg cggctgcgag tggaatcacc
      421 tgtgcccact cctgggtgat gaggcggtgc ctgcgcgcga acccgagcgc acccgccccg
      481 acgactcccg cggcgactcc ggcgagcgtt ccgtagccga tctcctcgat cacgatgcgg
      541 gccgcatcgc tccccgcgag cgcgccctct tcggtctccg ccaccgcgat cgcgatgaac
      601 agcaagggca cgcagaggcc gtcgttgagc ccgctctcga cgttcaggct ctgctgcacc
      661 cgcgacggca ggcgccggtc ggtgaccacg tccaggccca gcgccgcgtc ggtgggagcg
      721 aggacgacag ccagcacaaa cgcctcgccg aaggtgatct cctcgaacat cagggcgacc
      781 agcgtgccga ggatgagcgt aagggggagg ccgatcccga gcaagcgtga agggacgccc
      841 agctctcgcc gcaggacgcg catgttgatg cgcgaggcgt cgaggaagag cacgagagcg
      901 agggtcacct cggcgagtag ccgcaccgac ccgtcgccgc cgctcaggtt gatccagccg
      961 agcccctcgt tgccgaagac cagccccgcg ctgaggaaga cgatgggccc ggtgaggatc
     1021 gacttctcga tccagcgcga cagagacgcc agcacgagaa cgaggccggc gatgaccgcg
     1081 agcgtccagg cgtccattgg gtgatcctct agcagacgcc ccgtgaggcg tcatcggtag
     1141 tacgcggaag ccctagatca ggccgccggg gtaacgccat gggccagggc tcaacttgat
     1201 gccgcgcttg cggcagaact cgcgcaccga gtcgagcacc acgccgccgc cgccgtggag
     1261 ctgccggccg agcaggtcga tcacggcgac cccgtcgggc gcctggtcgc ccgagtattc
     1321 gagcgtcagc accacggtct cggcctctgc gaccgggatc gagtcggacg ctcgcaggcg
     1381 atcgacgagc agcacgtgcg agcggcctgt gtccccggtc actgcgaagc cgcgtccttc
     1441 cggcagcggc tccacgtgat ggccgcggag cgagatcagc tcctgcagct cgtcgagcat
     1501 ccggtgtgac gcttcggtct gctgctggcg cgagagcgtt cgcttcgcat ggccgaccgc
     1561 cttggcgggc acgccgatcg cgagcgaccc ctccgggatg tcggagacga cgtaggagct
     1621 cgaggcgacg atcgcctcgc ggccgatacg acagcccgcg tagacgactg tgcccaatcc
     1681 gacctgcgag cggtcctcga ggacgacccc ggcaaagcgg ttctcgaacc cctcgagcac
     1741 cgaatggccg atgttgtgcg tcacgatcat cgaccgcatc gtcaggaaca cctcgctgcc
     1801 gatcaggacc ggccggcaca cgttcacgaa cgcctccgag cccaggaagg tgtggtcgcc
     1861 gatcgtcagg gtggcccacg ggtcgtgatg gccgccgcca ccgatctgaa ccgacccgct
     1921 gcagtgaatc ccggcgccga ggtgggcgcg gcggcagcgg aggtcgagtc ccggcccgaa
     1981 atggctgacc tcgccgatcg cgacgacctc ggcgcagacg atgctgccgc cctgtccgat
     2041 ccgcacccga tcgccgagga cgatccgcgg cgccactacc agcgcgccgt cctccagcac
     2101 gacgccctcg ccgatcctgg ctccgtgctg gcggtaggtg gcgactttct cgtccgagct
     2161 gagtgaccgg aagcggttgg gatcactcgc gatcagctcg agaagcgcgg ggtcgacgac
     2221 gccggcgccc tcgtcctcac ctcgaagcga tgccggcagc gacacgttct gggtcgagat
     2281 gccggcgaag acaggatccg ggccgccgtc ctcggcggcc tgcgccgcga cgagcgcctc
     2341 gacgtcctcg gcggtgatga agccctgctt gtcgatctca tcgagcgcga ttccgtgttt
     2401 ctcggcgagc tcgaccgcct tcttcgtcgc cttgcgctgc gtcggtgctg cggtctccgc
     2461 cttctccgcc ggcgcggcag ttcggctcga gcgggcctgc tcgagctcgt ctgcgctctc
     2521 ggccacgagc gcgatagtcc gtccgagctc gacctcggcg cccgcatcgt agaggtggac
     2581 gagcgttccg tcgcccgggg cctcgaactc gagcgacgcc ttcgtcgtct cgaccacgca
     2641 gacgggctcg cctttgttca cttcggcgcc gtcgtcggcc agccactcga tcaggatggc
     2701 gacgtctgtg ttcgcgtcct cgcgcgtgag gatgatctcg agcatgcgcc tggcacgtta
     2761 acccgcgccg ttcgcgcgag ccacgggttc ccgcacgcta actacgcacg cctgcgagtg
     2821 cggcgacggc gtcgaccacg tcgttgacgc cggggagagc cttggcctcc agaccgggtg
     2881 ccgccgggat gatgccgtcg cgggcggcga tgcggcgcac gggcgcgcgc agatcgtccc
     2941 acgcgtgctc ttggatgcga gcggcgagct ccgcgccgta gccgccggtc agcccgccct
     3001 cctcggcggt gatcaccgcg ccgctccggg aggcggactc cagtaccgga tcgaggtcgg
     3061 gaggcagcag ccgagagagc gcgacgacct cgcagaagac ctcgtgctcg aggatcagct
     3121 cggtcactgc gtcgagcacg atcggcagca tgccgccgta cgtgacgatc gtcgccgcag
     3181 agtcggtgaa gtcgttcccc gagaagctga gcgccgggta gggcccgtcg ctctccacgc
     3241 agcggagctc gtcgacgaag ccgtcgacgg gccgccgggt ggggcgcccg tacatgagct
     3301 tgttctcgat caggaagacg ggtcgatcgt catcggtcgc ggcggccagg agtccgccgc
     3361 ggtcgtggca ctcgctcgcc gcgacgacga cgatgccggg aacgccgatc agcagcttct
     3421 ccagcgactg gctgtgcgtc ggtccgtagc cccggcgccc gcccatcggc gttcggacga
     3481 cgagcggcac cgtcacctgc tcgtcgaaca tctcgcggaa cttcgagatg ccgttgacga
     3541 tctggtcgta gccgagagcg acgaagtcgc cgaacatgat ctcgaggatc gggcgcttgc
     3601 ctcgcatcgc catccccgca gcgacgccga agagggaggc ctcgctgatc ggcgtcgtca
     3661 gcacacgctc cgggaagcgg tcgctcagcc cctgcgtgac cttgaacgcg ccgccgtacg
     3721 ggtcgagcac gtcctcgccg aggacgtaga cgttgtcccg cgtctccagg agctcgtgca
     3781 gcgcctcgtt gatgacgtgg acgcagcgct tctgtaccac tacaagtccc cgccggcgtc
     3841 gggcgcggcc tcggcctcgg cgatgatcgc tgccaggcgc tgctcgcacg attcctcgat
     3901 cgcgatgcgt tcggcgtcgc cgagcttcgc gccgctcaca cggagcggat cgcgcgtgcg
     3961 ccgctcgtcg atctcgcccg ggtcgcggaa gtcgtcaccc ttggagtggg ggctgaagcg
     4021 gtacgtgttg agcacgagga agaaggggga gcccgtcgtc cgcacgcgct cgacggcccg
     4081 ccccgccgtc tcgtggatct cgagcacgtc cgtcgtgtcg agctcggccg tcggcacgcc
     4141 gaaggccgcg gcccgctcgg ggatcgaccc cgcgaaggcg aggcggcgcg gcgtcgtctg
     4201 cgcgtactcg ttgttctcga cgacgaacag aagcggcagc tgccagaggg aggccatgtt
     4261 caagcactcg tagacggcgc cctggccaag cgttccgtcg ccgaggaaga cggtcgtgac
     4321 agcgccgctt ctcttctctt tctccgcgag cgcgatcccc gtggagacgg ggacgatgct
     4381 cccctgcacg ccgttcgagt agaagttccc cttgcagagg tgctggctgc cgcccttgcc
     4441 gccgcacgtc ccggtcgcct tccccatcac ctcgctgagg aggccgaagg catcacccgt
     4501 gaaggcgaga tagtggccgt ggcagcggtg gttggagaag acgacgtcac gctcgggctc
     4561 gaggtgcgcg atcacgcccg cggcgttcgc ctcctggccg atgtaggtgt gcgtcgtacc
     4621 caccagcttg ccctcggcga acatctcgag cagcgtctcc tcgaagcggc ggatgaagta
     4681 catccgctcg tagagctgct cgagcaagcc cgtccgggcg gccgctacgt cagtggcggt
     4741 gtcagtggcc atgggcgaaa gttagaggta gccgaggtcg gcgagccgcg ctgcgaccgt
     4801 tgcctcctcg tcctcgctca gctcgtgccg ctcggcgccc tcgagctccg cgtacagccg
     4861 ctcgcgcagc tcgggcggga cgtcgcctgg gcggcttgtc gtctcgcgcg ggtcgacgtc
     4921 gagcttgtac gcctcctcgc gcccgctctc gacgtggtgg atcagcttcc agggcggcat
     4981 gcgcacggcg agctgggaga gcgctcccat gtcggtgccc gagatgacgg ccggccggtc
     5041 gccgccctcg ctgccatcca ggagcggcag cagcgaggct ccgtccagtt cggggatggg
     5101 gatgcctgtc agttcgagca gcgtcggcat gaggtcggtc gagcgcacct gcgaccacac
     5161 ggtgcgaggg tcgactcgtc cgggtgccga gaggatcaac ggcacctgga cgacctcctc
     5221 gtagagcgtt gcgccgtgca tgtggtagat gcccttcacg tcgtccttct cggtgaagcg
     5281 ctcgccccac gactcgccgt ggtcggaggt gaacgcgagc accgtctgct cacgcagtcc
     5341 gagcgtctcg agctcctcga ggaacgaggc gaagagcacc tcggactggc gctccaccgc
     5401 tcgagcgtag ccctcgtgca gggtgagcaa gccgtcggcg gtgttcgact ggatcccggt
     5461 gatgatctct tccttcgccg ccagccactc ctttcgctcg gtgtggagga tgtcgtaggg
     5521 catgtgggtc gcccagctgt gcacgaagag caggaacggc tcctggcggc tctcgcgcag
     5581 ccacgcgatg acgccatcga gcgtctccgt cgtcccgagc acgttcgcct ccggccaccc
     5641 cttgaacaga tagttctcgt cgaacacgaa gctcgccacc tggtagcccg cggcggccgc
     5701 ggtcgtgaac agggtgggag tgcccggatc gagctctcgg tcccagtgga gcacgccgtg
     5761 gtggtgcggc tcgatgccgg tcgtcatcga cgtcatcgag gggcacgtcc acggcgcgac
     5821 gctgatcgcc tggtcgaagc gggcgccgcc ggctgcaagc tcatcgatcg tcttcgtgtt
     5881 gacgtggcgc gccccatagc agccgacgtg gtcggtccgc agggcatccg agacgtagac
     5941 gacgaggttc acgggcgggg gccgattcta cctgcgcctc ggaggcttcc ccagagcagg
     6001 aaatccctgc tcagagcgct ctaggcggct gtgagggcgg ctgcgaggct cgtacctcgc
     6061 atgtcttccg gccgc
//
LOCUS       DBNGNK010000373         4810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1709390,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000373 DBNGNK010000000
VERSION     DBNGNK010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1709390"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="AC150V_11100"
     CDS             complement(<1..288)
                     /locus_tag="AC150V_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_245904974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR3763506.1"
                     /translation="MALPPRVQEALGELVGSAKEGLLALSVGVGLGVLAELLEEEVVD
                     VGGPKGKHDPERTAVRHGHESGEVTLGGRRVQVERPRARTADGAAEVRLATY"
     gene            515..2425
                     /gene="abc-f"
                     /locus_tag="AC150V_11105"
     CDS             515..2425
                     /gene="abc-f"
                     /locus_tag="AC150V_11105"
                     /inference="COORDINATES: protein motif:HMM:NF000355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HIR3763507.1"
                     /translation="MAVLIATSLRKELSGDPLFDRVSFTVRRGDRVALSGPNGAGKTT
                     LLRMIAGETSIQAGELAFTKGTRVALHDQRQPRRSDLSLREYALSGATDLVAMEDELR
                     RLERAMADGAHDATTMRRYGEAQARLEHAGGWAWRDRAAAALRGLGFREADLDRPLDT
                     FSGGELTRASLARALAGDPDLLLLDEPTNHLDVESLEWLERELTSLDAAVILVAHDRW
                     FLEAVTTAVLELEGGEGLFFKGPWHAWRLERAARAAAAAKTVERVGADITRLERFVER
                     FRYKKSKAKQAQAKLTQIGRLEKERNRAGGELASLSKKRRTLGFEFLKPERSGRIVVE
                     AEGLTLQAGTKMLLSESTLVIERGEHVALVGPNGSGKTTLLETVLGNKEPRAGRIRLG
                     HGVLPAYFSQHDVELDERGTVLECAQAMTGLQRPQAQTLLGRFLFSGWEAQKKPVTAL
                     SGGERRRLSLAVVVASGANLLMLDEPTNHLDLDSREALEAALEAFPGTILLVSHDRAL
                     LDAVAERTVAIEDGALHSYEGGWADLVRVRTESDEAAAAEPMIEPSREKPLRKQVKAS
                     PSNPEAIELRRIEGRIEELERRVGELESTLAGDWTNMDVVAAHRAARAELQQLLARWE
                     ELFEATQSSTSS"
     gene            2526..3047
                     /locus_tag="AC150V_11110"
     CDS             2526..3047
                     /locus_tag="AC150V_11110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR3763508.1"
                     /translation="MTLTKDAHATVEPATLAEAAELHLDDVFGYLLYLTRDRTLADDL
                     AGATFEKALRSWSRFDPRRGSARAWLLGIARTTALDWFRAEERRRRREQAAAESELHE
                     EGFLEGLSPALERALAELNAGDREIVALRIVLELDGEQAAGVLGISPTAVSTRLSRAL
                     KRLEEKVKNDDVG"
     gene            3034..4110
                     /locus_tag="AC150V_11115"
     CDS             3034..4110
                     /locus_tag="AC150V_11115"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR3763509.1"
                     /translation="MTSAELVSELRAAKPVAADALRERVRVIATTEPEPRPSFLERLR
                     LPRPVLVAVPVAAALALVAAGAIGFVSSDEAPEVSPPIAVESSTGTDFGEARTQKGSE
                     GEDATGAAAPPTAGVGAATAPRQGAVAPSTDRAQRYAASLNLEVENIDTLSEATQRAL
                     SITRSLGGHVVTVSFATAESGTASMTLRVPSDKAQDAVARLSQLGTIRAQQVQIDDLQ
                     ESIDALDAEIVGLREQIARLSTRLANPNLSDDARSVVRARLETARAELASLQASRRAT
                     AGEAALATVQLSLQTASESRVVPAPSRFDRALDEAVGVLAWEGIALLYAAIIAAPFVL
                     AGVAAFVVRRGVRRRDEERLLASS"
     gene            complement(4120..4695)
                     /locus_tag="AC150V_11120"
     CDS             complement(4120..4695)
                     /locus_tag="AC150V_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013829053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR3763510.1"
                     /translation="MAGRILIAEDETIIRLDLRELLERSGYVVCAEAKDGEEAVALAR
                     SEEPDLALLDVKMPRLDGIDAARRILAERPIPIVMVTAYGEDELVARAIEAGVFGYLV
                     KPFRESDLLPAIQTARARHEELLALRAEAESLSEALAARKAIERAKGLLMERDGLTEE
                     DAFKRLRKASQISGRPLKIVAEALIATLDPN"
BASE COUNT          759 a         1710 c         1647 g          694 t
ORIGIN      
        1 gtaggtggcc agacgcacct ccgctgcgcc gtctgcggtg cgagcgcgcg gccgctccac
       61 ctggacgcgc cggccgccga gcgtcacctc gcccgactcg tgaccgtgac ggaccgccgt
      121 gcgctcagga tcatgcttgc ctttcggccc gccgacgtcg acgacttctt cttcgagcag
      181 ctcggccagc acgcccaggc cgacgccgac gctcaaagcc aacagcccct ccttcgccga
      241 accgaccagc tccccgagcg cctcctgcac acgcggcggc agcgccaact cctcctgtcc
      301 gacctgctac agcgatacgg tcctcacggc gatcctcctg tctcgaacga cgacttcggc
      361 actcgccacg gtcgctcggc gaccggacga ggcgggagga ccgccacctc aagttctacg
      421 agctacggga caacctctac atgccccttg ggggagaact tgagagcgat tcccccggtc
      481 gagggatacg gtttcgcggt ctacgcttgc cgaaatggcc gtgctgatcg caacaagtct
      541 ccggaaggag ctgtcgggag acccgctctt cgaccgggtc tccttcacgg tgcgccgcgg
      601 cgaccgcgtc gcactgtccg ggccgaacgg cgccgggaag acgacgctgc tcaggatgat
      661 cgccggggaa acgtcgatcc aggccggcga gctcgcgttc acgaagggga cgagggtggc
      721 tcttcacgat cagcggcagc cgcggcgtag cgatctctcg ctgcgcgaat acgcgctctc
      781 cggcgcgacg gacctcgtcg ccatggagga cgagctgcgc cggctcgagc gggcgatggc
      841 ggacggggct catgacgcga cgacgatgcg tcgctacggc gaggcacagg cccgcctgga
      901 gcacgcgggc ggctgggcgt ggcgtgatcg ggccgccgca gcgctccgtg ggctcggctt
      961 tcgcgaggcc gacctcgatc gcccgctcga cacgttctcg ggcggcgagc tgacacgcgc
     1021 ctctctcgcc cgcgcgctcg cgggcgatcc cgatctcctg ctcctcgacg agccgacgaa
     1081 ccacctcgac gtcgagagcc tcgagtggct cgagcgtgag ctgacgtccc tcgatgctgc
     1141 tgtgatcctc gtcgcacacg accgctggtt cctcgaagcc gtgacgacgg ctgtactcga
     1201 gctcgaagga ggcgaggggc tcttcttcaa gggcccgtgg cacgcctggc ggctcgagag
     1261 ggccgcccgc gccgcggcgg cggcgaagac cgtcgagcgg gtcggagcgg acattacacg
     1321 cctcgagcgg ttcgtcgagc ggttccgcta caagaagagc aaggccaagc aagcacaggc
     1381 caagctgacg cagatcgggc ggctcgagaa agagcgcaac cgcgccggag gggagctcgc
     1441 cagcctcagc aagaagcggc ggacactcgg gttcgagttc ttgaagcccg agcgatcggg
     1501 acgaatcgtt gtcgaggcgg aagggctcac tctgcaggcg gggacgaaga tgctgttgag
     1561 cgagtcgact ctcgtgatcg agcgtggaga gcatgtcgcc ctggtcggcc cgaacggctc
     1621 gggcaagacg actctgctcg agacggtgct cgggaacaaa gagccgcgtg ccggccgcat
     1681 acggctcggg cacggcgtgc tgcctgctta cttctcgcag cacgacgtcg agctcgacga
     1741 gcgcggcacc gtgctcgagt gcgcccaggc gatgaccggc ctgcaaaggc ctcaggcaca
     1801 gacgttgctc ggccgcttcc tcttctccgg gtgggaggcg cagaagaaac cggtgacggc
     1861 gctgtcgggc ggcgagcggc gccggctctc actggcggtc gtcgtcgcgt ccggcgcgaa
     1921 cctgctcatg ctcgacgagc cgaccaacca cctagacctg gacagccgcg aggcactgga
     1981 agcggccctc gaggcgttcc cgggaacgat cctgctcgtc tcccacgacc gcgctctgct
     2041 cgacgcggtg gccgagcgaa cggtcgcgat cgaggacggc gcgctgcatt cctacgaggg
     2101 cggctgggca gacctcgtcc gcgtgcgaac cgagagcgac gaggcggccg cggcggagcc
     2161 gatgatcgag ccgtcgcgcg agaagcccct gcggaagcag gtgaaagcga gcccctccaa
     2221 tcccgaggct atcgagctga ggcgcatcga gggacgtatc gaagagctcg agcgacgggt
     2281 cggcgagctg gagtcgaccc tggcgggcga ctggacgaac atggacgtcg ttgccgcgca
     2341 tcgcgccgcc cgggccgagt tgcagcagct cctggcacgt tgggaggagc tgttcgaggc
     2401 gacgcagtcc tccacttcga gctgacagcc gccgatacag caagtaacag actgttgctt
     2461 gccgctcgag tgctgcgaga tgtgacgccg gaggggccgg gcggcgtatt gaggacgtgc
     2521 acgccatgac gctcaccaag gacgcacacg ccacggtcga gcccgccact ctggcggagg
     2581 cggccgagct gcacctcgac gacgtcttcg ggtacctcct gtacctgacc cgcgaccgaa
     2641 cgctcgccga cgacctcgcc ggcgcgacgt tcgagaaggc cctgcgaagc tggagccgct
     2701 tcgacccacg ccgcggcagc gcccgagcat ggctgctggg catcgcccgc accaccgcgc
     2761 tcgactggtt ccgcgcggag gaacgccggc ggcgccgcga gcaggcggcg gccgagagcg
     2821 agctccacga ggagggcttc ctcgaagggc tttctcccgc gctcgagcgg gcactcgccg
     2881 agctgaacgc cggtgaccgg gagatcgtcg cgctgcggat cgtgctcgag ctggacggtg
     2941 aacaggcggc tggcgtgctc ggcatcagcc cgacggcggt ctcgacccgc ctcagccggg
     3001 ccctcaaacg actcgaagag aaggtgaaga acgatgacgt cggctgaact ggtctccgaa
     3061 ctcagagccg ccaagcccgt cgcggcagac gcgctcagag aaagggtgcg ggtgatcgcg
     3121 acaacggagc ccgagccgcg tccgtccttc ctggagcggc tccggctccc gcggccggtg
     3181 ctcgttgccg ttcccgttgc cgccgccctc gctctggtgg ccgccggcgc gatcggcttc
     3241 gtcagctcgg acgaggcgcc ggaagtctcg ccgccgatcg cggtcgagag ctcgactggc
     3301 accgacttcg gcgaggctcg cacccagaag gggagcgagg gagaagacgc gaccggggcc
     3361 gccgccccgc ccaccgcggg tgtcggagcc gccacggcac caaggcaggg cgccgtcgct
     3421 ccctccaccg atcgcgccca gcgatacgcc gcctccttga atctcgaggt cgagaacatc
     3481 gacacccttt ccgaggcgac gcagcgtgcg ctctcgatca cccgctcgct cggcgggcac
     3541 gtcgtcaccg tctccttcgc gaccgccgag agcggtacgg cctcgatgac cctgcgcgtg
     3601 ccgagcgaca aggcgcagga cgctgtggct cgactctccc agctcggcac catccgcgcg
     3661 caacaggtgc agatcgacga tctccaggag agcatcgacg cgctagacgc cgagatcgta
     3721 gggctgcggg agcagatcgc ccgcctcagc acacggctgg ccaaccccaa cttgtccgac
     3781 gacgcgcgca gcgtcgtccg cgcccggctc gagacggcgc gggccgagct cgccagtctg
     3841 caggcctctc gccgcgcgac cgccggcgag gcggctctcg cgaccgtgca gctctcgctc
     3901 cagacggctt ccgagtcccg cgtcgtaccc gcgccgtcgc ggttcgaccg tgcgctcgac
     3961 gaggccgtcg gcgtcctcgc ctgggaaggc atcgcgctcc tctacgcggc gatcatcgcg
     4021 gctccattcg ttctcgcggg cgtggccgcc ttcgtcgtcc gccgcggcgt gcggcggcgc
     4081 gacgaggagc gcctgctggc ctcctcgtaa ggcgatgcct cagttcgggt cgagcgtggc
     4141 gatcagcgcc tccgccacga tcttgagcgg ccgacctgag atctggctcg ccttgcgcag
     4201 gcgcttgaag gcatcctcct ccgtcagtcc gtcgcgctcc atcagcaggc ccttcgcccg
     4261 ctcgatcgcc ttgcgcgcgg cgagcgcctc cgagagcgac tccgcctctg cgcgtagtgc
     4321 caacagctcc tcgtgccgcg cgcgcgccgt ctggatcgca ggcaagagat cggactcgcg
     4381 gaagggcttc acgaggtagc cgaacacgcc cgcctcgatc gcccgcgcga ctagctcgtc
     4441 ctcgccgtac gccgtgacca tcacgatcgg gatcggccgc tccgcaagaa tgcgccgggc
     4501 ggcgtcgata ccgtccaggc gcggcatctt cacatcgagg agggccaggt ccggctcttc
     4561 cgagcgcgcc agcgcgaccg cctcctcgcc gtccttggcc tcggcgcaga cgacgtagcc
     4621 ggatcgctcg agcagctcgc gcagatcgag gcggatgatc gtctcgtcct cggcgatgag
     4681 gattcgtccg gccatcggaa cagtgtgtca gcccggcacg caggccgacc cggaggggtc
     4741 tttactcgcg tgccgcctgc gaagactgcc actcgtcatg gagcgcccgg acgtcgccga
     4801 ttccttcgaa
//
LOCUS       DBNGNK010000374         6999 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1709414,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000374 DBNGNK010000000
VERSION     DBNGNK010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6999)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6999)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6999
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1709414"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC150V_11125"
     CDS             <1..322
                     /locus_tag="AC150V_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SSI family serine proteinase inhibitor"
                     /protein_id="HIR3763511.1"
                     /translation="ETSLTIVYGKDGGQPEPSEEWTLTCEPQGGTHPDPDAACARLAS
                     VGIGAFEPVPDGTACAEIYGGPQRAEVSGKLDGETIDATFERRNGCEIDRWDTLEEVL
                     PAAS"
     gene            complement(319..1125)
                     /locus_tag="AC150V_11130"
     CDS             complement(319..1125)
                     /locus_tag="AC150V_11130"
                     /EC_number="2.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016603 - glutaminyl-peptide
                     cyclotransferase activity [Evidence IEA];
                     GO_process: GO:0017186 - peptidyl-pyroglutamic acid
                     biosynthetic process, using glutaminyl-peptide
                     cyclotransferase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HIR3763512.1"
                     /translation="MRVLLVLGLTASCLCLTGAGAAADTVVAPKALKVQVLRSFPHDP
                     AAFTQGLELDGGRLIESTGLYGRSSLRIVGRTSGRVVQRRDLDARLFGEGVTVVDGRI
                     VQLTWKEQVAPVYERKTLRPLKSFRYQGEGWGICHDGRFLVTSDGSSTLTFRNPKNFR
                     PVRTVQVEVGGSAVVRAGLPSGPLSSLNELECVGKAVYANVWQTDVIVRIDSASGRVT
                     AVIVAAGLLPAASAAKAGVLNGIAYDSVRRVFLLTGKLWPRLYEVRFVRR"
     gene            1174..1824
                     /locus_tag="AC150V_11135"
     CDS             1174..1824
                     /locus_tag="AC150V_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763513.1"
                     /translation="MTDTACDFELPHYIELLENAQSAGYRFARFGEEPEPGDLFLRHD
                     IDLTLDAAVRMAELEAELGVTATYLLMTESIFYNLASPEGVAAIERIRELGHPVGLHA
                     VYPTVELDDRFDPVVAWHNPQPEYMSLPIDGVVNVMEERYFSPATYRSDSNQHWRAGC
                     PHADLRAGAFPWLQMLIHPAIWIYEGRTMGETMRSMLDAERERRLGQLPGDRIYLE"
     gene            1841..2203
                     /locus_tag="AC150V_11140"
     CDS             1841..2203
                     /locus_tag="AC150V_11140"
                     /inference="COORDINATES: protein motif:HMM:NF025444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4259 domain-containing protein"
                     /protein_id="HIR3763514.1"
                     /translation="MGTWGVGPFDNDDDDCLLHEALDEVADAGADEYIEAPWAAMAIA
                     AAEIVAAGSGKADPGLPEVAADWVRRTDGLLSDAHRLLALRAVENALSSELAVLWQDS
                     ADGAYWRARTDELRERLT"
     gene            2200..3147
                     /locus_tag="AC150V_11145"
     CDS             2200..3147
                     /locus_tag="AC150V_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR3763515.1"
                     /translation="MSRTDRPLRVVHCPVNTAGVPWSNVQALRARGVDATLVVFNRYK
                     LHPEADWSLERRGGLARQQLTQFRALAKLLPHTDVFHFYFGLTLVPQKIQYPLLRLFD
                     KRCVMHYLGRDMRHKSPAELAPGRKAGARIVGSYDARRWVPDAEVIPPGIGISQIAPS
                     PPPRETRPVILHAPSSRTRKGTEHVIAACEGLDADLELVEGLHHEEAFQRYRNADIVV
                     DQLNAGWYGLLAIECMALGKPVLGFLRADSTRWTAEGFGIEVPIVPVTAETLRERLRP
                     LVTDAAERRRLGAASRAYAERVHDIERTADRLLDVYARL"
     gene            3170..4639
                     /locus_tag="AC150V_11150"
     CDS             3170..4639
                     /locus_tag="AC150V_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR3763516.1"
                     /translation="MALAAQLRRLGSQSLVYGLGGLVSRILAVLLLPLYTRYLTPEDY
                     GRVETLVALVAVLTIALRLGIASAFFRFWFDAETPAEHRTVLRTSFWFTMSMATIGLA
                     LLILFADPISRLFFGTDDAADLVIASGVALWANMNYEQMTAVFRVEQRPGAFVAASLA
                     NVFLTIGATLLLVVALEQGALGVIVGNLSGTLLVYLVLLAYRREQLGLELSRPLLREM
                     NRFGLPLVPSALFLWATNFSDRFFLVTLADASEVGLYSVGVRIASAMVLLLTAFRMAW
                     PAFAYSIDDDGEAKRTYAYVLTYLVAISSWVALGLAVLSPWLVDWLAAPSFAEASRVV
                     GPLAFAAVALAGYIVLMIGIGRTKRTQFVWVVTGAGAAVNVALNLSLIPRYGMMGAAV
                     ATVAAFVTMFLAMAWWSQRVFPTPYQWRRVTTAVAAALVLAIGAKLLDLGFGLSLLVA
                     VAYPLVLLPLGFYLAPERRAIAQAITLRRRRGRPGAHAP"
     gene            complement(4593..5702)
                     /locus_tag="AC150V_11155"
     CDS             complement(4593..5702)
                     /locus_tag="AC150V_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3763517.1"
                     /translation="MTSGQLEILAIASVTAVACALVGVFLVLRRMSLISDAISHSILV
                     GIALGFFVTGSVTSPWLVVGATLVGVLTVALVELIHRTRLVKEDAAIGLVFPALFSAG
                     VILISRYADGVHLDIDAVLLGELAFAPFDRLELFGWDVGPVSLVVMGVILVVDALAIA
                     LFWKELKISTFDSALALALGLAPGFVHYGLMTLVSVTAVGAFNAVGSILVVALMIAPP
                     AAAYLLTDRLWVMTALACAIGAASAIVGYWIAYVLDASIAGSMAGAAGALFAAAWILA
                     PGRGLLAARRRRARQRTEFAQTMLAIHLANHEGAPDQERESAVEHLGDHLRWSPDFAQ
                     RIVRGAERRGLITRSNGRLELTERGRRAARDAVAA"
     gene            complement(5699..6802)
                     /locus_tag="AC150V_11160"
     CDS             complement(5699..6802)
                     /locus_tag="AC150V_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020611195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR3763518.1"
                     /translation="MASGALALLFDDYTLRVVALGAAVLGIVSGALGTFAVLRRQALL
                     GDAISHAALPGVALGYLAAGTKSSLALMVGAALAGFLGTLVVMWIVRTSRVKQDAALG
                     IVLAVSFGIGLVLLTFIQRRPDASQAGLDRFLFGQAATLLREDVITMAVVGGAALVVM
                     LAVWKQLKLLAFDPDYGATLGLRMRAVDVLFTGLLVVAIVIGLQTVGVVLMSAMVVAP
                     AAAARQWTNRLGGMVALSACFGALAGAVGAIASAEADRLPTGPTIVLVITAIVVLSLL
                     LAPQRGLVPAAVRRHLMRRRLGLYAVLSDLAALERQHPGEARGHSAAVIGVMEEVDAA
                     GVLGELERRGLVVREGDDAWLLTDSGRQVVSHT"
     gene            complement(6802..>6999)
                     /locus_tag="AC150V_11165"
     CDS             complement(6802..>6999)
                     /locus_tag="AC150V_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese ABC transporter ATP-binding protein"
                     /protein_id="HIR3763519.1"
                     /translation="RELRARGKTIVAVHHDLQTVTEYFDQVTLLNVRRIASGPVDEVF
                     TDENLRLTYGGRAAVFGTNGR"
BASE COUNT         1127 a         2451 c         2396 g         1025 t
ORIGIN      
        1 cgagacgtcg ctgacgatcg tctacgggaa ggacgggggc cagccggagc cgagtgagga
       61 atggaccctc acgtgcgagc cgcagggggg cacgcatcct gaccccgacg ccgcgtgcgc
      121 gcgtctcgca tcggtcggca tcggcgcgtt cgagccggtt ccggacggga cggcctgcgc
      181 cgagatctac ggcggcccgc agcgagccga ggtttcgggg aagctggacg gcgagaccat
      241 cgacgcaacg ttcgagcgcc gcaacggatg tgagatcgac cgctgggaca cgctcgaaga
      301 ggtgcttccg gcggcctcct agcggcggac gaaccgcacc tcgtacagac gcggccagag
      361 ctttccggta aggaggaaga cccgccgtac agagtcgtag gcgatcccgt tcaggacgcc
      421 cgccttcgcc gccgaggctg ccgggaggag gcccgcggcg acgatcacgg cggtgacgcg
      481 gccgctcgcg gaatcgatac ggacgatcac gtccgtctgc cacacattcg cgtaaacggc
      541 cttgccgacg cactcgagct cgttgagcga actgagtggc ccgctgggaa gtccggctcg
      601 gacgacagcc gagccgccga cctcgacctg gacggtccga accggccgga agttcttggg
      661 gttccggaat gtaagggtcg acgagccgtc gctcgtgacg aggaagcggc cgtcgtggca
      721 gatcccccac ccctcgccct ggtagcgaaa ggacttcagc ggacggagcg tcttccgctc
      781 gtagacgggc gcgacctgct ctttccaggt cagctgcacg atccggccgt cgaccacggt
      841 cacgccttcg ccgaacagcc gcgcatcgag atcgcgccgt tgcacgacgc gcccactggt
      901 gcggccgacg atacggaggc tcgatcgccc gtagaggccg gtgctctcga tcaggcggcc
      961 gccgtcaagc tccagaccct gcgtgaaggc agccgggtcg tgcgggaagg agcggagcac
     1021 ctgaaccttg agtgccttcg gtgcgacaac ggtgtcggcg gcggctccgg cgccggtcag
     1081 gcacagacag ctggcggtca gaccgagaac gaggagcacg cgcaccccca caaggtgcct
     1141 gaagagcccg tgccctcgct agagtcgccg ccgatgaccg acaccgcctg cgacttcgag
     1201 ctgccgcact acatcgagct cctcgagaac gcgcagtcgg ccggctaccg cttcgcacgc
     1261 ttcggcgagg aaccggagcc gggcgatctc tttctgcgcc acgacatcga cctcacgctc
     1321 gatgccgccg tccgcatggc ggagctcgag gcggagctgg gcgtcaccgc gacctatctg
     1381 ctgatgacgg agtcgatctt ctacaacctc gcctcgcccg agggggtcgc cgcgatcgag
     1441 cggatccggg agctcggcca cccggtcggc ctgcacgccg tctacccgac cgtcgagctc
     1501 gacgaccggt tcgatccggt cgtcgcctgg cacaaccccc agcccgagta catgtcgctc
     1561 ccgatcgacg gtgtcgtcaa cgtgatggaa gagcgctact tctcgcccgc gacctatcgc
     1621 tccgactcga atcagcactg gcgcgccggg tgcccgcacg ccgacctccg cgccggggcg
     1681 ttcccgtggc tccagatgct gatccatccc gcgatctgga tctacgaggg gcgcacgatg
     1741 ggagagacga tgcgctcgat gctcgacgcc gagagggaac gccggctcgg ccagttgccc
     1801 ggcgaccgga tctacctcga gtgagcagga tcgaacgctg atgggcacgt ggggcgtggg
     1861 cccgttcgac aacgacgacg acgactgctt gctccacgag gcgctcgacg aggtagccga
     1921 tgccggcgcg gacgagtaca tcgaggcccc gtgggcggcc atggcgattg cagccgccga
     1981 gatcgtcgct gcgggttcgg ggaaggccga tccggggctc cccgaggtgg ccgccgattg
     2041 ggtgcgccga accgacggcc tcctgtccga cgcacaccgt ctgctcgcgc ttcgcgcggt
     2101 cgagaacgcg ttgtcgtccg agctcgccgt cctctggcag gactccgcgg acggcgcgta
     2161 ctggcgggcg cgcaccgatg aattgcgcga gcggctgacg tgagccgcac ggatcggcct
     2221 ctgcgcgtcg tgcactgccc ggtcaacacg gcaggcgtgc cgtggtcgaa cgtccaggcg
     2281 ctccgcgcgc gcggcgtcga cgcgacgctc gtcgtcttca accgctacaa gctccatccc
     2341 gaggcggact ggtcgctcga gcgccgcggc ggacttgcgc gacagcagct cacgcagttc
     2401 cgagcgctgg ccaagctcct gccgcacacc gacgtgttcc acttctactt cgggctcacg
     2461 ctcgttccgc agaagatcca gtacccgctg ctgcgcctgt tcgacaagcg atgcgtaatg
     2521 cactacctcg gccgagacat gcgccacaag agccccgcag agctcgcgcc cggccgcaag
     2581 gcgggcgcgc gcatcgtcgg cagctacgac gccaggcgat gggttccgga tgcagaggtg
     2641 atcccgccgg gaatcggcat ctcgcagatc gcaccgtcac cgccgccgcg cgagacgcgt
     2701 cccgtcatcc tccatgcacc ttcgtcgcgc acgcggaagg gaaccgagca cgtgatcgcc
     2761 gcatgcgagg gcctcgacgc cgacctcgag ctggtcgagg gcctgcatca cgaggaggcc
     2821 ttccagcgct accggaacgc cgatatcgtc gtcgaccagc tgaacgcggg ctggtacggg
     2881 ctgctcgcga tcgagtgcat ggcgctcggc aagcccgttc tcggctttct gcgagcagac
     2941 tcgacccgct ggacggcgga gggattcggg atcgaggtac cgatcgtccc ggtcacggcc
     3001 gagacgctgc gcgagcggct ccgaccgctc gtcaccgacg ccgcagagcg acggcgactc
     3061 ggtgcagcat cccgcgcgta cgcagagcgg gtgcacgaca tcgagcggac ggcagaccgc
     3121 ctgctcgacg tctacgctcg gctttgatgc ccgcgcagga ccgcgtgtag tggcgctcgc
     3181 cgcgcagctc aggcggctgg gctcgcagtc gctcgtctac ggcctcggcg ggctcgtctc
     3241 gcgaatcctc gcggtcctgc tgctaccgct ctacacgcgg tatctgacgc ccgaggacta
     3301 cgggagggtc gagacgttgg tggcgctcgt cgcggtgctg acgatcgccc tcaggttggg
     3361 gatcgccagc gcgttcttcc gcttctggtt cgacgcggag acgccggccg agcaccgcac
     3421 cgtcctgcgc acgtccttct ggttcacgat gtcgatggcc acgatcgggc tggcacttct
     3481 gatcctcttc gccgatccga tctcgcggct cttcttcggc accgacgacg ccgccgacct
     3541 cgtgatcgcg tccggggtcg cactctgggc caacatgaac tacgagcaga tgacagctgt
     3601 cttccgggtc gagcagcgtc cgggcgcgtt cgtcgcggcg agcctcgcca acgtcttcct
     3661 gaccatcggc gcaacgcttc tgctcgtcgt cgcgctcgag cagggcgccc tgggcgtcat
     3721 cgtcgggaac ctctcgggca cgctgctcgt gtacctcgtc ctactcgcct accggcgcga
     3781 gcagcttggc ctggagcttt cacgtccgct gttgcgggag atgaaccgct tcgggctgcc
     3841 gctcgttcca tccgctctgt tcctctgggc gacgaacttc tccgaccgct tctttctcgt
     3901 cacgctggcg gatgcctccg aggtcggtct gtactcggtc ggcgtgagga tcgcgtccgc
     3961 gatggtgctc ctgctcacgg ccttccggat ggcctggccc gcgttcgcgt actcgatcga
     4021 cgacgacggg gaggccaagc gcacgtacgc ctacgtcctc acctacctgg tggcgatctc
     4081 ctcctgggtc gcactcggac tggcagtcct ctctccctgg ctcgtcgact ggctcgccgc
     4141 gccctcgttc gccgaggcgt cgcgtgtcgt cggcccgctc gcgttcgcag ccgtcgccct
     4201 ggccggctac atcgtcctga tgatcggcat cgggcgcacg aaacggacgc agttcgtctg
     4261 ggtcgtcacc ggcgccggag ctgccgtcaa cgtcgcgctc aacctgagcc tgatcccgcg
     4321 ctacgggatg atgggtgccg ccgtcgcgac ggtcgcagcg ttcgtgacga tgttcctcgc
     4381 catggcctgg tggtcgcagc gggtcttccc gacgccgtac cagtggcgcc gcgtcacgac
     4441 cgccgtcgct gccgccctcg tcttggcgat cggcgccaag ctgctcgacc tcgggttcgg
     4501 gctctccctg ctcgtcgccg tcgcctaccc gctcgtcctg ctgccgctcg gcttctacct
     4561 cgcgcccgag cgccgggcga tcgcgcaggc gatcacgctg cgacggcgtc gcgggcggcc
     4621 cggcgcccac gctccgtgag ctcgagccga ccgttgctgc gggtgatcag cccgcgccgc
     4681 tcggcaccgc gcacgatgcg ctgagcgaag tccggcgacc agcgcaggtg atcgcccaga
     4741 tgctcgaccg cgctctcgcg ttcctggtcg ggcgcgcctt cgtggttggc aaggtgaatg
     4801 gcgagcatgg tctgggcgaa ctccgtccgc tgcctggcgc gacggcgtcg cgcggcgagc
     4861 agtccccggc caggagcgag gatccacgcc gccgcgaaga gggcgccggc agcccccgcc
     4921 atcgacccgg cgatcgacgc gtcgaggaca tacgcgatcc agtagcccac gatcgccgac
     4981 gcggcgccga tggcacacgc gagcgccgtc atgacccaca gtcgatccgt cagcaggtag
     5041 gcggcggccg gaggcgcgat catcagcgcc accacgagga tcgagccgac cgcgttgaac
     5101 gcaccgacgg cggtgacgga gacgagggtc atgaggccgt agtgaacgaa cccgggcgcg
     5161 agcccgagcg cgagtgcgag cgccgagtcg aacgtcgaga tcttcagctc cttccagaac
     5221 agcgcgatgg cgagagcgtc gaccacgagg atgacgccca tgacgacgag cgagacgggg
     5281 cccacgtccc agccgaagag ctcgagacgg tcgaacggcg cgaaggcgag ctccccgagc
     5341 aggactgcgt cgatgtcgag gtggacgccg tccgcgtagc ggctgatcaa gatcacgccg
     5401 gccgagaaga gtgccgggaa gacgagcccg atcgccgcgt cctccttgac gaggcgggtg
     5461 cgatggatga gctcgacaag ggcgacggtc aacacgccga cgagtgtcgc cccgacgacg
     5521 agccagggag acgtgaccga gccggtgacg aagaagccca gcgcgatccc gaccaggatc
     5581 gagtggctga tcgcgtcgga gatgagggac atccgccgca gcacgagaaa gactccgacg
     5641 agcgcacagg cgacggcagt caccgacgcg atggcgagga tctcgagttg gcccgacgtc
     5701 acgtgtgcga caccacctga cgcccgctgt cggtgagaag ccaggcgtcg tctccctcgc
     5761 ggacgaccaa tccgcgtcgc tcgagctccc cgaggacgcc ggcagcgtca acctcctcca
     5821 tcacgccgat cacggccgca gagtggcctc gagcctcacc cgggtgctgg cgctcgagcg
     5881 cggccagatc cgacaagacg gcgtagaggc cgagccgtcg acgcatgagg tggcgccgga
     5941 cggccgccgg cacgagaccg cgctgcggcg cgagaagcag gctgagaacc acgatcgcgg
     6001 taatgacgag gacgatcgtg gggccggtcg gcagccgatc tgcttccgcc gaggcaatcg
     6061 cgccgactgc ccctgcgagc gccccgaagc atgccgaaag agccaccatg ccgccgagcc
     6121 ggttcgtcca ctgacgcgct gcggcggcgg gcgcgacgac catcgcgctc atcaggacga
     6181 cgccgaccgt ctgcagcccg atcacgatcg ccacgacgag caggccggtg aagagcacgt
     6241 cgacggcgcg catccgcagg ccgagcgtgg cgccgtagtc gggatcgaag gcgagcagct
     6301 tcagctgttt ccagaccgcg agcatgacta cgagcgcggc gccgccgacc acggccatcg
     6361 tgatcacgtc ctcccgaagc agggtggcgg cctggccgaa gaggaagcgg tcgagccccg
     6421 cttgggacgc gtcgggacgc cgctggatga acgtgagcag cacgaggccg atgccgaagg
     6481 agaccgcgag aacgattccg agggcggcgt cctgcttcac acgtgacgtg cggacgatcc
     6541 acatcacgac gagcgtgccg agaaagcccg cgagagccgc gcccaccatg agcgcgagcg
     6601 acgacttcgt tcccgccgcg aggtagccga gcgcgacgcc cggcagcgcg gcgtgcgaga
     6661 tcgcgtcgcc gagcagggcc tggcggcgga gaacggcgaa cgtgccgagc gcgccgctga
     6721 cgatgccgag caccgcagcc ccgagcgcga cgacgcggag agtgtagtcg tcgaacagca
     6781 gtgcgagcgc cccgctcgcc atcagcgccc gttcgtgccg aagacggccg cacggccgcc
     6841 gtaggtcaag cgcaggttct cgtcggtgaa gacctcgtcg accggtccag acgcgatccg
     6901 ccgcacgttc aggagcgtca cctggtcgaa gtactcggta accgtctgga ggtcgtggtg
     6961 gacggcgacg atcgtcttcc cgcgcgcccg cagctcacg
//
LOCUS       DBNGNK010000375         4187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_853408,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000375 DBNGNK010000000
VERSION     DBNGNK010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_853408"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /gene="murJ"
                     /locus_tag="AC150V_11170"
     CDS             complement(<1..1356)
                     /gene="murJ"
                     /locus_tag="AC150V_11170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR3763520.1"
                     /translation="MEEPGQGRRLARSTAFFAAATGLSRLLGLVREIVTAYFFGVTGR
                     INAFTVAYQLPNLFRALVADMALTGAFVPIFSELLTKGDRARAWRFASTIFWLTLLGL
                     SGLTALLVLIAPLLIRPFGDPGGDFDLAVGLSRVLYPTIVLLGLTGIVVGILNAYDNF
                     TVPALTPVFWNLAIIFGLLIGIPAADGESAELYVYAFAVLVATLIQFLLPLPWLKGLD
                     GRLRFVVDWRDPLVRRFFALMLPVALTLGLVNVNAVVDTLFASRLIDPEKAPTAIDKA
                     FRLYMLPQGMFSVAVATVLFPLLSRLAARDELGEFRRTVDLGLRQIGFLLIPASFVTA
                     VLVEPIVRLVYERGAFTEADTPAVSGALAAFAIGLTFNGWLLMLTRAFYSLQSNWIPT
                     TVALGTVGLNAALDAVFYRLGIWGIPLATSVVNILGVAVLMIAMSRRVGIEPFGQTLA
                     SL"
     gene            1489..1755
                     /gene="rpsT"
                     /locus_tag="AC150V_11175"
     CDS             1489..1755
                     /gene="rpsT"
                     /locus_tag="AC150V_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR3763521.1"
                     /translation="MANIKQQKKRIRLAARQRQENLRYRSTVKTLTRRLEDAVHDGDA
                     ETVSREHRELVRSIDRASSQGALHQNAAARKKSQAARLVAGTSD"
     gene            complement(1768..2757)
                     /gene="holA"
                     /locus_tag="AC150V_11180"
     CDS             complement(1768..2757)
                     /gene="holA"
                     /locus_tag="AC150V_11180"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR3763522.1"
                     /translation="MSESPELKAVYLLTGSDRPKIQRALRRLRARVGEDGVEHLSAAD
                     SSGADAAAACNALGLFGGEARLVVVDGVEAWKKTDVDDLVSYLADPAPGTVLALVAAE
                     LKADGPLAKACAKHGQVLTYSVQKRELQKWVAEQFKLAGARAEPDACAALLHLVGDDP
                     QALATEVQKLATWADGEPIGDREVEQLVAATAETPTFTMTDAWAKRDRAEALEVSEAV
                     FEREGKPPRDTAPRLLGALTGHLARLQRLKRLADQGVPVRDAASQLRMHPFYAKKVAA
                     QAEGFSYEELHDATVRLAELDLALKGGSRLAPELELQRALIDLGREAGDSRPT"
     gene            complement(2754..>4187)
                     /locus_tag="AC150V_11185"
     CDS             complement(2754..>4187)
                     /locus_tag="AC150V_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR3763523.1"
                     /translation="VRAGVAGVLASLAWLAARPRDRWHFLALGALVLLVWTPAMLLDP
                     GFQLSFAAVAAILLSVGRLRQFHAGVPLPWRIVELAGIAAVCGAATAPILWLQFGSVP
                     LWTVPANALAEPAMPLLLGFGLAAAVVNPFSSSAAAALAMLAGWCAAWLAFCARLIAG
                     LPYAETTSPLPLLALAGAAPTIVLLRRASRRLRRRALALGGFALSLTVALVWSVGGPP
                     AIAPPAGLRVSFLDVGQGDATLLQVPEGSVLVDQGPPEAGITDRLRRLNVRSLSALVL
                     THPQRDHVGGAADVLRSLPVEAVLEPALSFESAEDDSALAAARSRGVGILVGRAGQSF
                     RLGRLRLDVLWPDGPGPPSTDPNENAIVLLASYGRVEVLLTADVESHVTRRLPVGAVD
                     VLKVAHHGSRDDGLGSQLRALRPSVAVVSAGAENEYGHPHETTLRALSAVPGLDVYRT
                     DLDGDVVVESNGRAITVRTTRGVRSVG"
BASE COUNT          769 a         1402 c         1481 g          535 t
ORIGIN      
        1 gagtgaggcg agggtctggc cgaacggctc gatgcccacc cgacgagaca ttgcgatcat
       61 gagaacggcc acgccgagga tgttgacgac cgacgtggcg agggggatcc cccagatccc
      121 gagccgatag aagacggcgt cgagcgcggc gttcagtccg accgtcccca aggcgacggt
      181 ggtcgggatc caattcgact gcaggctgta gaaggctcgg gtgagcatca gaagccagcc
      241 gttgaacgtc aagccgatcg cgaacgccgc cagcgcgccg gagacagcag gtgtgtccgc
      301 ctccgtgaag gcgccgcgct cgtagacgag ccgaacgatc ggctcgacca ggacggctgt
      361 gacgaagctg gcgggaatca gcaggaaccc gatctggcgg agacccaggt cgaccgtgcg
      421 gcggaactcg ccgagctcgt cacgggccgc gagccgagac agcagcggga agagtacggt
      481 cgccaccgcg acggagaaca tgccctgtgg aagcatgtag aggcgaaagg ccttgtcgat
      541 cgccgtcggc gccttctcgg gatcgatcag ccgcgacgcg aacagcgtgt cgacgactgc
      601 gttcacgttg accagcccga gcgtcaacgc gacgggcagc atcagcgcga agaagcgtcg
      661 cacgagcgga tcacgccagt cgacgacgaa acgtaagcgt ccgtccagcc ctttgagcca
      721 cgggaggggc agaaggaact ggatcaacgt cgccacgagt accgcgaagg cgtagacgta
      781 gagctcggcg ctctctccgt cagcggccgg gatgccgatc agcagaccga agatgatcgc
      841 caggttccag aagacggggg tcaaggcagg aaccgtgaag ttgtcgtagg cgttgaggat
      901 cccgacgacg atccctgtca gaccgagcag cacgatcgtc gggtagagga ctcgtgacag
      961 accgacggcg aggtcgaagt cgccacccgg atcgccgaag gggcggatca ggagcggcgc
     1021 gatcagcacc agcagtgctg tcagaccgct gaggccgagc agggtgagcc agaagatcgt
     1081 ggacgcgaac cgccatgccc gcgctcggtc gcccttcgtc agcagctcgc tgaagatcgg
     1141 gacgaacgcc cccgtcaggg ccatgtctgc gacaagcgcg cgaaagagat tcggcagctg
     1201 gtacgcgaca gtgaaggcgt tgattctgcc cgtcacgccg aagaaatagg ccgtcacgat
     1261 ctcgcggacg agaccgagca gccgagacag accggtcgcc gccgcgaaga acgccgtcga
     1321 acgggcgaga cggcgtccct gaccgggctc ctccacgtcg gtcgagtata ggaggggcgg
     1381 ccgaaggtgc ctgcgagcgc gggcctgcgg cgagtcccgc cggaaccctc tggggacgcc
     1441 gctctgctac gatccggcgg cccgcgcaag cgggcgtttc gacgctctat ggccaacatc
     1501 aagcaacaga agaagcgcat ccgactcgct gctcggcagc gtcaggagaa cctgcgctac
     1561 cgctcgacgg tgaaaactct gacccgccgc ctcgaggatg cagtgcacga cggcgacgcg
     1621 gagacggtct cccgcgagca ccgcgagctc gttcgttcga tcgaccgcgc gtcgagtcaa
     1681 ggcgcgctcc atcagaacgc ggcggcccgc aagaagtcgc aggctgcccg gctcgtcgcc
     1741 ggtacctccg actagaagag gggcagtcta ggtgggccgg ctgtcgccgg cctcgcgacc
     1801 caggtcgatc agagcacgct gcaactcgag ctcgggcgcg agccgactgc ctcccttgag
     1861 agcgagatcg agctcggcga gacggaccgt cgcgtcgtgg agctcctcgt aggagaaccc
     1921 ctcggcctgc gcagccacct tcttggcata gaaggggtgc attcgcagtt gagacgcggc
     1981 gtcgcgcacg ggcactccct ggtcggcgag tcgcttgaga cgctggagcc gggcgaggtg
     2041 tccggtcagg gcccccagca gccgcggagc cgtatcccgc gggggctttc cctctcgctc
     2101 gaagaccgcc tcgctcacct cgagcgcttc ggcgcgatcg cgcttcgccc aggcatcggt
     2161 catcgtgaag gtcggcgtct cggcggtcgc ggcgacgagc tgttccacct ctcgatcgcc
     2221 gatcggctcg ccgtcggccc aggtcgccag cttctgcacc tcggtcgcga gcgcctgggg
     2281 atcatccccg acgagatgga gcaacgccgc acatgcgtcc ggctcagccc gcgcaccggc
     2341 aagcttgaat tgctcggcga cccacttctg gagctctcgc ttctgtaccg agtaggtgag
     2401 gacctgcccg tgcttggcac acgccttggc cagcggcccg tcagccttga gctccgcggc
     2461 cacgagggcg aggacggttc ccggagcggg atcggcgaga taggagacaa ggtcgtccac
     2521 gtcggttttc ttccaggcct cgacgccgtc gacgacgacc aggcgggcct caccgccgaa
     2581 cagcccgagc gcgttgcagg ctgccgcagc gtccgcaccg gaggaatcgg cagccgaaag
     2641 gtgctcgacc ccgtcttccc cgacgcgggc gcggagacgg cgcaacgctc gctggatctt
     2701 cggtcggtca ctaccggtga gcaggtagac ggccttaagc tccggcgact cactcacccg
     2761 acggagcgta ctccacgggt cgttcggacg gtgatcgccc gtccattcga ttcgacgacg
     2821 acgtcgccgt ccaagtccgt tcggtacacg tccagcccgg gaaccgcgct tagcgctcgc
     2881 agggtcgtct cgtgcgggtg gccgtactcg ttctcagcgc cggcagagac gaccgcgaca
     2941 ctcgggcgaa gagctctgag ctgggagccc agcccgtcgt cgcgcgagcc gtgatgggcg
     3001 actttgagga cgtccaccgc gccgaccggg agccgccgag tgacgtgcga ttcgacgtcg
     3061 gcggtgagga gcacctcgac gcggccatag gaggcgagca ggacgatcgc gttctcgttc
     3121 gggtcggtgc tcggcgggcc gggcccgtcg ggccacagga cgtccaggcg gagccgcccg
     3181 agccgaaagg attgccccgc ccgtccgacg aggatcccga caccgcggga gcgagccgcg
     3241 gcgagagcgg agtcgtcctc ggcgctctcg aacgacagcg cgggctcgag cacggcctcg
     3301 accggcagcg accggaggac gtcggcggca ccgccgacat gatcgcgctg ggggtgagtc
     3361 agaacgagcg cggacaggct ccggacattc agccggcgga gccggtcggt gatgcctgcc
     3421 tcgggtggcc cttgatcgac tagcaccgac ccttcgggca cctgcaacaa ggtcgcgtcc
     3481 ccctgaccca cgtcgaggaa gctgacgcgc aatccggccg ggggagcgat cgcgggcggg
     3541 ccacccacgc tccagacgag ggcgaccgtg agcgacaacg caaaccctcc cagtgcgagc
     3601 gctcgacgcc ggaggcgccg agatgctcgt cggagcagga cgatcgtggg cgccgccccc
     3661 gcgagcgcca gtagcggcag cggcgacgtc gtctcggcgt aggggagccc cgcgatgagc
     3721 cgcgcgcaga aagccagcca ggctgcacac cagcccgcga gcatggcgag cgccgccgcg
     3781 gccgacgacg agaacgggtt cacgacggca gccgcgagcc cgaacccgag cagcagcggc
     3841 atcgccggct cggcgagcgc gttcgccggg actgtccaga gcggcaccga gccgaactgg
     3901 agccagagga tcggggccgt cgcggcgccg cagacggcgg cgattccagc cagctccacg
     3961 atccgccacg gcagcggaac gcccgcgtgg aactgccgga gccgcccgac gctgaggagg
     4021 atcgcagcaa cggcggcgaa cgagagctgg aatcccgggt cgagcagcat cgcgggcgtc
     4081 cagacgagaa ggacgagagc gccgagggcg aggaaatgcc acctgtcccg cggacgcgcc
     4141 gcgagccagg cgagggatgc gagaactcct gcgacgccgg cccgcac
//
LOCUS       DBNGNK010000376         3902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_636453,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000376 DBNGNK010000000
VERSION     DBNGNK010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_636453"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC150V_11190"
     CDS             <1..332
                     /locus_tag="AC150V_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763524.1"
                     /translation="SDLIGREIEIRSEPLVDAADEPVPPDVALGVFASREGAVWTTAS
                     CSLTDPGALLAISDEPRGNWIKVLIGIGILGVVLLFSVRRLRRRQLQQAAQATSGNGG
                     PPDGDPA"
     gene            413..1249
                     /locus_tag="AC150V_11195"
     CDS             413..1249
                     /locus_tag="AC150V_11195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3763525.1"
                     /translation="MTFAHVLVVGAGQMGGGIAQVVAASGREVSLHDAMPGAVARALE
                     AMGRSLAKLAEKGGPDPEATLARVRAVDELAPADLMIEAVVEDLDVKEKVFRAADELL
                     PPSAVLASNTSSIPITSLAALTSRPDRVIGMHFFNPVPVLQLVEVIRAVQTSDETAAA
                     ITGLAEELGKVPAEARDFPGFVSNRILMPFVNEAAYALMEGVAEAEAIDTIAKLGFAH
                     PIGPLALADLIGLDTCVAIMEVLHEGFGDQKYAPCPLLRQYVAAGRLGRKSGRGFYSY
                     DG"
     gene            complement(1246..2430)
                     /locus_tag="AC150V_11200"
     CDS             complement(1246..2430)
                     /locus_tag="AC150V_11200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR3763526.1"
                     /translation="MAIGYAALAVMLVGSWSVVASRTSSITPFLFACLFATAFLAERI
                     ALPLSPRGWYTVSTPVVVLTGLLGGPAAGALSGAATALGDGDAAWRRRVAFGGLDAVR
                     GFAAGLIGLMPVAGAVGAGMRAALAVSCALLLNAAGRLVIHQARRIGQPLAFRRGALA
                     DALEAVAAVPILALILQSYEAHGAVLVVLALVGLLVALWLATEAFSRSRAELEAEQEL
                     ARTDPLTGAPNRRALEEELARVYARVMRGERAAAVLVLDVDHFKLINDEHGWDGGDAV
                     LVELVSRLGEGLRDTDLLARRGGEEFAVVATGVDSPAAVRRLAESLRLRAGDAPLGQN
                     IDVTISVGAALLDESVEPAAAERRANVALAQAKTTRNTVVVWDELEASASTAPSLTPA
                     TT"
     gene            2638..3804
                     /locus_tag="AC150V_11205"
     CDS             2638..3804
                     /locus_tag="AC150V_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR3763527.1"
                     /translation="MDFTLTPEQREIQVLAREVADAEIAPNAAAWDRERRFPAELVAK
                     LGPLGLMGVCVPEEYGGAGADFLSYILVLEELSRADAGVGVTVAVHTSAVTLPILMFG
                     TDEQRSRFVPPLARGETLGAFALTESESGSDAGSLKTAAVPVGDGWRISVAKQWITNG
                     THAGTFVLFARTDPEASGSRGVSCFLLDREHVEITREEEKLGLNSSSTADLRLVDAFV
                     GRDRLLHEERRGFHVAMATLDGGRIGIAAQAVGIAQAAFDLARGYALERHQFGRRIAD
                     NQAIQFKLADMGTEIDAARLLTYRAAALKQQGLPHTEQGAKAKLFASEMARRQTAEAI
                     QILGGYGYTKEFPAERYYRDAKVTEIYEGTSEVQRIVIARHILERAERSVAASG"
     gene            3785..>3902
                     /locus_tag="AC150V_11210"
     CDS             3785..>3902
                     /locus_tag="AC150V_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017536381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR3763528.1"
                     /translation="MLPRPADEPVRLTRIYTRSGDAGETSLGDGSRTSKLDLR"
BASE COUNT          604 a         1367 c         1365 g          566 t
ORIGIN      
        1 cttccgatct gatcgggcgc gagatcgaga ttcggtccga gccgctcgtc gacgcggccg
       61 acgagcccgt gccgcccgac gtcgcgctcg gcgtcttcgc gtcccgggag ggagctgtct
      121 ggacgactgc ctcctgcagc ctgaccgacc ctggggccct gctcgcgatc tccgacgagc
      181 cgcgcggaaa ctggatcaag gttctgatcg gcatcgggat cctcggcgtt gtgctccttt
      241 tctccgtccg gcgcctgcgg cgccgtcagc tgcagcaagc ggcgcaggcg acgtcgggca
      301 acggcgggcc gcccgacggc gaccccgcct agcgcgggcc cacccgtgtc gttgcctcgc
      361 cccgtcttgc gccggcggga acgggctccg ggccggatgt agggtgccgc cgatgacatt
      421 cgcgcacgtc ctggtggtcg gggcaggtca gatgggcggc ggtatcgccc aggtcgtcgc
      481 ggcctcggga cgcgaggtct cgcttcatga tgcgatgccc ggggcggttg cgcgtgcact
      541 cgaagcgatg ggtcgatccc tcgccaagct cgcggagaag ggcgggcccg accccgaggc
      601 cacgcttgct cgagttcggg cagtggacga gctcgccccg gccgatctca tgatcgaggc
      661 tgtcgtcgag gatctggacg tgaaagagaa ggtcttccgc gcagcggacg agttgctccc
      721 gccgagcgcg gtgctcgcct cgaacacgtc gtcgattccg atcacgtcgc tcgctgccct
      781 gacgagtcgg cccgaccgcg tcatcggcat gcacttcttc aacccggttc cggtgctcca
      841 gctcgtggag gtgatccgcg ccgtccagac atccgacgag accgccgcgg ccatcaccgg
      901 ccttgccgaa gagctgggta aagtcccggc cgaggcgcgc gactttcccg gttttgtctc
      961 gaaccggatc ctgatgccct tcgtgaacga ggcggcgtac gcgctcatgg agggcgtcgc
     1021 cgaggcggag gcgatcgaca ccattgcgaa gctcggcttc gcgcatccga tcgggccact
     1081 cgctctcgcc gacctgatcg gactcgacac gtgcgtggcg atcatggagg tgctccacga
     1141 aggcttcggc gaccagaagt acgcgccgtg tccgctgctg cggcagtacg tcgccgcggg
     1201 gcgtctcgga aggaagagcg gtcgcggctt ctacagctac gacggctagg tcgtcgccgg
     1261 ggtcagcgac ggagccgtcg acgcgctcgc ctcgagctcg tcccagacga cgacggtgtt
     1321 acgcgtcgtc ttggcctggg cgagggcgac gttcgcccgc cgttcggccg ccgccggctc
     1381 gacggactcg tccagcagcg ccgccccgac ggagatcgtg acgtcgatgt tctggccgag
     1441 cggagcatcg cccgcccgaa ggcggaggct ctccgcgagc cgccgcacgg cggccggcga
     1501 atcgacgcct gtggcgacca cggcgaactc ctcgccgccc cgtcgtgcca gaagatcggt
     1561 gtcgcgcagc ccttcgccga ggcggctcac gagctcgacg agcacggcat cgccgccgtc
     1621 ccaaccgtgt tcgtcgttga tgagcttgaa gtgatcgacg tcgagtacca acacggccgc
     1681 ggcccgctcg ccccgcatga cgcgggcgta gacgcgggcc aactcctcct cgagcgcgcg
     1741 gcggttcggc gcgccggtga gcggatcggt tcgggcgagc tcctgctcgg cctcgagctc
     1801 cgcccggctt cgactgaagg cctccgtcgc gagccagaga gcaacgagta gtccgacgag
     1861 cgcgagaacg accaggacgg cgccgtgcgc ctcgtagctc tggaggatca gcgcgagaat
     1921 cggcactgcc gccaccgcct ccagggcgtc ggccagagct ccgcgccgga acgcgagggg
     1981 ctgcccgatc cgccgcgcct ggtggatgac gagccggccg gcggcgttca agaggagcgc
     2041 gcagctcacg gcgagcgcgg cccgcattcc tgcgcccacc gcgccggcga ccggcatcaa
     2101 tccgatcagg cccgcggcga acccgcgaac ggcgtccagc ccgccgaatg cgacgcgacg
     2161 gcgccacgct gcatcgccgt ctcccagcgc tgtcgccgcc ccggacaacg cgcctgccgc
     2221 gggcccgccc agcaggcccg tgaggacgac gaccggcgtc gacaccgtgt accagccccg
     2281 cggggagagc ggcagcgcga tccgctctgc gaggaaggca gtggcgaaca ggcaggcgaa
     2341 cagaaacggt gtgatcgagg acgtgcggct cgcgaccacg ctccacgagc cgacgagcat
     2401 caccgcgagc gcggcgtacc cgattgccac ccaggtcgcg ctgtctggac ttctcgaccc
     2461 gccggtacgg ccggcggatc ccgtgctcgg acccactaca ggcaccccct cacaattcag
     2521 agccgcactg ggtcctgcag atcttctgcc gagggccggt acgggcgcat ccctcggccg
     2581 ggggacgggc gggcgaggat ttccgctcag agcgcacgcc gtagacttgg tcgcggtatg
     2641 gacttcactc tcacgcccga gcaaagagag atccaggtgc tcgcgcgcga ggtcgccgac
     2701 gccgagatcg ctccgaacgc ggcagcgtgg gatcgcgaac gtcgcttccc ggcggagctg
     2761 gtcgcgaagc tcggaccgct gggactcatg ggggtctgcg tgccggagga atacgggggc
     2821 gccggcgccg acttcctctc gtacatcctc gtcctcgagg agttgtcgcg cgccgacgcg
     2881 ggagtgggcg tgacagtcgc cgtccacaca agcgcggtga cgctgccgat cctcatgttc
     2941 ggcacggacg agcagcgcag ccgcttcgtc ccgccgctcg ctcgtggaga gacgctcggc
     3001 gcgttcgccc tgaccgagtc ggaatccggc tcggatgccg gctcgctcaa gaccgcagcc
     3061 gtccccgttg gcgacggctg gcggatcagc gtcgcgaagc agtggatcac gaacgggacg
     3121 cacgcgggaa cgttcgtgct cttcgcgcga accgatcccg aggcgtccgg gtcacgcggc
     3181 gtgtcctgtt tcctgctcga tcgagagcac gtcgagatca cgcgcgagga ggagaagctg
     3241 ggcttgaact cctcgtcgac ggccgacctc cgcctggtcg acgccttcgt cggacgcgac
     3301 cgactcctcc acgaggaacg gcgggggttc catgtcgcga tggcgacgct ggacggcggc
     3361 cgcatcggca tcgccgcaca ggcggtcggg atcgcgcagg cggcgttcga tctcgcgcgg
     3421 ggctatgcgc tcgaacggca ccagttcggg cgccgcatcg ccgacaacca ggcgatccag
     3481 ttcaagctgg ccgacatggg aaccgagatc gacgcggccc ggctgctcac ctaccgcgca
     3541 gccgcgctca agcagcaggg tctcccgcac acggaacagg gggcgaaggc gaagctgttc
     3601 gcgtccgaga tggcgcgccg gcagacggcc gaagcgatcc agatcctggg cggctacggc
     3661 tacacgaagg agttcccggc agagcgctac taccgcgatg ccaaggtcac ggagatctac
     3721 gagggaacga gcgaggtgca gcgaatcgtc attgcccggc acatcctcga gcgcgcggag
     3781 agatctgttg ccgcgtccgg ctgacgagcc cgttcgactc acgcgcatct acacccgcag
     3841 cggtgatgcc ggcgagacgt cgctcggcga cggcagcagg acgtccaagc tcgacctgcg
     3901 ta
//
LOCUS       DBNGNK010000377         3137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_565612,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000377 DBNGNK010000000
VERSION     DBNGNK010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_565612"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..1156
                     /locus_tag="AC150V_11215"
     CDS             215..1156
                     /locus_tag="AC150V_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012407.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR3763529.1"
                     /translation="MIYERYRIACLSGNGIGPEVMAEASRALAHVSHLHGFGVDEIHP
                     PFGAEAVARFGHPLPAATRRATLDADAVLVAGASEPALHAVRAELDVAASVIRVLRED
                     GGGLTLFAPLYDEAAAWTIARAFSSARAASGSLVSVGISSDWRRLVDAATERNDGVDV
                     VDLALADALHALTPGSDDLGVVLTERALADALAEAPSRERDARRMRASGCLSPVGPGL
                     FGPAHGSARDIAGQGVANPSEMLLAASLMLGEGLGRRAGAQTLETSLAEALRRPARTP
                     DLAGPGKAATTREFVDVVLDLLSTVRRDTEFALGGAS"
     gene            1153..2886
                     /gene="ilvB"
                     /locus_tag="AC150V_11220"
     CDS             1153..2886
                     /gene="ilvB"
                     /locus_tag="AC150V_11220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR3763530.1"
                     /translation="MSRMTGADAILRSLEAEGVDVMFGIPGGAILPTYDAIARGTTVR
                     HVLARHEQGAGHMAQGYARASGRPGVALATSGPGATNLVTPIADAWMDSTPLVCITGQ
                     VRTHLIGTDAFQECDITGITLPIVKHSWLVQDVRDIPQAMKAAFHVARTGRCGPVLVD
                     VPRDVQEAEFDFHYPASVDLPGWRPPAKVHPLQIREAAKRIADAEKPVLYVGGGTING
                     DACAELLELAEAGGLPVITTLMGKGAFPETHPLHFGWPGMHGPKWSNLAMNSCDLLVA
                     IGARFDDRVTGKLSAFAPGATVVHLDIDPAEIGKLRAADIPVVGPLKRALQELAGKVA
                     GHRADGVPAPDAWVQQIADWRDEFPLRYGPSRDYLKPQTVVERLQSLIAGDDVIVTTG
                     VGQHQMWAMQYILSERPRSFISSGGLGTMGYGIPAAIGAKAARPEATVVCVDGDGCFQ
                     MTAQELATAALEGLPIVVVLINNGFLGMVTQWQDMFFSERRSHVHLTDSVPDYAKLAE
                     AFGGVGITVRTEDELGPALEEALASPRTVVVDCRVEPDEQCFPMIPAGAAALDMVEYT
                     DEDSVELKESA"
     gene            2883..>3137
                     /gene="ilvN"
                     /locus_tag="AC150V_11225"
     CDS             2883..>3137
                     /gene="ilvN"
                     /locus_tag="AC150V_11225"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011430944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR3763531.1"
                     /translation="MKHTLSVLVEDKPGALARISTMFARRGFNIDSLAVGPTERPGVS
                     SITLRVDCSQHSLEQIEKQIHKLVNVLRVTELVPGEAVERE"
BASE COUNT          493 a         1105 c         1082 g          457 t
ORIGIN      
        1 cggccgcggc atcggcatcg agctcctgct gattctcccc cgctaggggg agacatcgaa
       61 caagcgccgg aggcgcagac gccgagaaat tgcaccacag gtgcgcccgc aggcgtgccg
      121 ataacgattc gaagggaaac gacgagcgag tgacgaccgc cagacaccga gacacagagc
      181 caccggcgac gcgccgtgca gccgaggagc gcccgtgatc tacgagcgct accgcatcgc
      241 ctgcctgtcc gggaacggga tcggtccgga ggtgatggcc gaggccagcc gtgctctggc
      301 gcatgtctcg caccttcacg gcttcggcgt cgacgagatc catccgccgt tcggagccga
      361 ggcggtcgcc cgcttcgggc acccgctgcc cgccgcgacc cggcgcgcga ctctcgacgc
      421 cgacgcggtg ctcgttgcgg gagccagtga gccggcgctg cacgccgtga gggccgagct
      481 cgacgtggct gcatcggtga ttcgagttct tcgcgaggac ggcggcgggc tcacgctgtt
      541 cgcgccgctc tacgacgagg cggccgcctg gacgatcgcg cgcgccttct catcggcccg
      601 cgccgccagc gggagcctcg tctccgtcgg aatctccagc gactggcgga ggctcgtcga
      661 tgctgcgacc gagcgaaacg acggcgtcga cgtcgtcgat ctcgcactcg ctgacgcgct
      721 gcatgcgctg acgccgggat cggacgacct gggcgtcgtt ctgacagagc gcgccttggc
      781 cgatgcgctc gcagaagccc cgtcgcggga acgagacgct cgacgcatgc gcgcatccgg
      841 ctgtctctct cccgtcggcc ccggcctctt cgggcccgct cacgggtctg cgcgcgacat
      901 cgcggggcag ggcgtcgcca acccgagcga gatgctgctc gcggcctcac tgatgctcgg
      961 agagggcctg ggacgccgcg cgggcgcgca gaccctcgag acgagtctcg ccgaggcgct
     1021 gcgccggccg gcgcgaacgc cggacctcgc cgggcccggc aaggctgcga cgacgcgcga
     1081 gttcgtcgac gtggtgctcg acctgctgtc taccgtacgc cgtgacaccg agttcgcgct
     1141 gggaggcgcc tcatgagccg catgacagga gcagacgcga tcctccgctc gctcgaggca
     1201 gagggagtcg acgtgatgtt cggcatcccc gggggcgcga tcctgccgac ctacgacgca
     1261 atcgcccgtg gcacaacggt gcggcacgtg ctcgcccgcc acgagcaggg cgccggccac
     1321 atggcgcagg gctacgctcg agcctccggc cggccgggcg tcgcgctcgc gacgtcggga
     1381 ccgggcgcca cgaacctcgt cacgccaatc gcggacgcgt ggatggactc gacgccgctg
     1441 gtctgcatca cggggcaggt gcgtacgcac ctgatcggca ccgacgcctt tcaggagtgc
     1501 gatatcaccg gcatcacgct cccgatcgtc aagcactcgt ggctcgtgca ggacgtccgc
     1561 gacatcccgc aggcgatgaa ggccgctttc catgtcgctc ggacgggtcg ctgcggaccg
     1621 gtactcgtcg atgttccacg cgacgtccag gaggccgagt tcgacttcca ctaccccgct
     1681 tccgtcgacc tccccggctg gcggccccct gcgaaggtgc atccgctgca gatccgcgaa
     1741 gcggccaaga gaatcgccga cgccgagaag cccgtcctgt acgtgggcgg cgggacgatc
     1801 aacggcgacg cgtgcgcgga actcctcgaa ctggccgagg cgggtggcct gcccgtgatc
     1861 acgacgctga tgggcaaagg cgcgttcccg gagacccacc cgctccactt cgggtggccg
     1921 ggcatgcatg gacccaagtg gtcgaacctg gcgatgaaca gctgtgatct cctcgtcgcg
     1981 atcggcgccc gcttcgacga ccgggtcacc ggcaagctct cggccttcgc gcccggcgcg
     2041 acagtcgtcc atctcgacat cgacccggcc gagatcggca agcttcgcgc agccgacatt
     2101 ccggtcgtgg ggcccctgaa gcgggcgctg caggagctcg ccggcaaggt ggcgggccac
     2161 agggcagacg gcgtcccggc tcccgatgcc tgggttcagc agatcgccga ctggcgcgac
     2221 gagttcccgc tccgctacgg gccctcccgc gactacctca agccgcagac cgtcgtcgag
     2281 cggctgcagt cgctgatcgc cggcgacgac gtgatcgtca cgaccggcgt cgggcagcac
     2341 cagatgtggg cgatgcagta catcctctcg gagcggccgc gctcgttcat ctcatccggc
     2401 ggcctcggga cgatgggcta cgggatcccc gcggcgatcg gcgccaaggc cgctcggccc
     2461 gaggcaaccg tcgtctgcgt cgacggggac ggctgcttcc agatgaccgc gcaggagctg
     2521 gcgacggcgg cgctcgaggg gctcccgatc gtcgtcgtcc tgatcaacaa cggcttcctc
     2581 gggatggtta cgcagtggca ggacatgttc ttcagcgagc ggcgctcaca cgtgcacctc
     2641 acagactcgg tgccggacta cgcaaagctg gccgaggcgt tcggcggagt gggcatcacg
     2701 gtgcggaccg aggacgagct cggcccggca ctcgaagagg cactcgcctc gccgaggact
     2761 gtcgtcgtcg actgccgcgt cgagccggac gagcaatgct tcccgatgat cccggccggc
     2821 gccgcagctc tggacatggt cgagtacacg gacgaagact cggtcgagct gaaggagtcc
     2881 gcatgaagca cacgctgtcg gttctcgtcg aggacaagcc gggggcgctc gcccgcatct
     2941 ccacgatgtt cgcgcgacgc gggttcaaca tcgactcact cgcggtcgga cccaccgagc
     3001 gaccgggcgt ctcgtcgatc accctgcgcg tcgactgctc gcagcactcg ctcgagcaga
     3061 tcgagaagca gatccacaag ctggtgaacg tcctccgcgt caccgagctg gttcccgggg
     3121 aggccgtgga gcgtgag
//
LOCUS       DBNGNK010000378         4936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_565631,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000378 DBNGNK010000000
VERSION     DBNGNK010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_565631"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1479)
                     /locus_tag="AC150V_11230"
     CDS             complement(127..1479)
                     /locus_tag="AC150V_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR3763532.1"
                     /translation="MLDPKVCKAYDVRGIYPTELDEAGAYAIGRAYVEHFEPRVIAVG
                     RDMRLAAPAMAAATIDGAADGGADVVDIGMVGTEMVYNTVDELGLDGGICVTASHNPK
                     EYTGMKIVRRGALPVGGDSGLNDVRDRALAGFGPVTRRGEVREEDVFPSFVDKVLSFV
                     EPTAIGPLRVVIDAANGMAGAMLPPVLERLPGIDAVCCHFDPDGSFPNHEPNPLLPEN
                     REFIVEQTRTENADLGVAYDGDADRCFFVDDTGEFVPGDFTTALLAESMLAKEADGDG
                     RVIYDVRASWAVPRTIESAGGVPLVNRVGHAFIKHRMRKEGAVFGGEVSAHYYFRDFA
                     QADSGVVPFLLMLELISERGQKLSEILAPYRDRYFLTGEINTPVADVPLKLQELKERY
                     AAEGGRVSHLDGISVDFDDWHFNVRPSNTEPLLRLNLEALSRATMEQKREEVLALIRA
                     "
     gene            1541..4471
                     /locus_tag="AC150V_11235"
     CDS             1541..4471
                     /locus_tag="AC150V_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR3763533.1"
                     /translation="MERKLAAVLFVDIVGSTELVAGLDPEVARRRVASFFDRVSACIS
                     VHGGTVEKFAGDAVMAAFGIPHAHEDDAERAARAALAIRDAVRALELEVRIGIEAGEV
                     VTGGADSPFATGGPFNLAARLQAEAEPGQILLGPAAHSLTRGRMTTEPLGSRELRGFA
                     QPIDVWRLLGAPDDAGRRMSVTAPFIGRDAELDLLENTWTRAVRDRRAQLFTIYGEPG
                     VGKSRVAAEFVDSVEGATVLVGRCLPYGEGITYWPLAEMVKLAAGISDDDPADEAMEK
                     LRACCGEDAVADLLALAAGVLEAVSGERSAQEIAWAAEEWAGELASAQPLVLGFEDIH
                     WAEEPLLDLIEHLADRVRSVPLLILCLARPELLDVRPNWGGGRLRATAIELEPLVSEE
                     RETLVDALLGDHELAPDVRRRLVEKTEGNPLFLEETVRMLLEDGEQAPERIPDTVQAL
                     IAARIDGLPREAKDVLQRGAVIGRVFWEGAVAALAPGSDDVAEALEELVDREFLTLEA
                     RSSISAEEAYRFKHVLIREVAYAGIAKGDRAVLHEGFAEWLKERAVEEMIEIRAYHLD
                     EAARLSEELEGSVPPELAAEAAAALERAGRRALAREANRPARNLLRRAVELEPTLERR
                     YDAAKAAWRLADLPTVSDEMVQVRRLAAAEGDRRIEGAALTALAEVALLRDADLPRGR
                     ELVEEALELLGEEPSENRFDALEMRAMVGWWLGDLDENERYARLALDVGRALGRRDLE
                     ASALDYLAGSSIVRLDLETGSELLASAIELSTASGNPVARAWSLHTQSRICELQGDLD
                     EAERVAEEARELFAQAEVSWALGRVLNRLGWIARWRGDLKTSERRFRDSIQLMKGIED
                     RGTLCESQRSLAELLVQLGRLEEAEQLALEARTTVGGHDQNSRATTRMALGIVRAAQG
                     RYEEAEALLREALEIIEHSDAKLVRAEVLAAMIAFLHERGRAADAAGYEAELAALRST
                     SVPA"
     gene            complement(4423..>4936)
                     /locus_tag="AC150V_11240"
     CDS             complement(4423..>4936)
                     /locus_tag="AC150V_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR3763534.1"
                     /translation="REEALDAVVEQLGTLRERLEQKSRAVPFGVEVMGRVRDLGSLDD
                     VLQIARRTGWVRPVLDFAHMHATSDGAYLDEEPFATALAAVDELLADGAAFHIHFSDI
                     AFANRNETKHLPYGAGTLRAEPLRDALARFDRPATVISESPDEASSQAIREILYAGTD
                     VERSAASSAS"
BASE COUNT          758 a         1663 c         1776 g          739 t
ORIGIN      
        1 gctccggcgc ctcggtcacg gtgcgagcgg tccgcgagcg atcgacgtcg agaattcgtc
       61 gtacgccgaa catcctcgcc cggatctccg tgatgtctgc agcagtgtcg tcatggcagc
      121 ccccggctag gcgcggatca gggcgagcac ctcttcacgc ttctgctcca tcgtcgcccg
      181 cgaaagcgcc tcgaggttga gacgcagaag cggctccgtg ttcgacggcc tgacgttgaa
      241 gtgccagtcg tcgaagtcga ccgagatgcc gtcgaggtgg gacacacggc cgccctcggc
      301 ggcgtaccgc tccttcagct cctggagctt cagcgggaca tccgccaccg gcgtgttgat
      361 ctctcccgtc aggaagtagc ggtcgcggta cggagcgagg atctccgaga gcttctgccc
      421 ccgttcggag atcagctcga gcatgagcag gaagggaacg acgcccgaat ccgcctgcgc
      481 gaagtcgcgg aagtagtaat gggccgagac ctcacctccg aagacggcgc cctccttgcg
      541 catgcggtgc ttgatgaagg cgtggccgac gcggttcacg agcggcactc caccggccga
      601 ctcgatcgtc cgcggcaccg cccagctggc gcgcacgtcg tagatcacgc gtccatcccc
      661 gtcggcctcc ttggcgagca tcgactcggc caggagcgcc gtagtgaaat cgccgggaac
      721 gaactcgcct gtgtcgtcga cgaagaaaca ccgatccgcg tcgccgtcgt aggcgacgcc
      781 cagatcggca ttttccgtcc gggtctgctc gacgatgaac tcccggtttt ccggcagcag
      841 cgggttcggc tcgtgattcg ggaagctgcc gtcgggatcg aagtggcagc agacggcgtc
      901 gataccggga aggcgctcga ggacgggggg gagcatcgct ccggccatcc cgttggccgc
      961 gtcgatgacc acccgcagcg ggccgatagc cgtcggctcg acgaacgaga gcaccttgtc
     1021 gacgaaactc gggaagacgt cctcctcccg cacctcgccg cgcctggtca ccggtccgaa
     1081 gccggcgaga gcgcggtcgc ggacgtcgtt caagcccgag tcgccgccca cgggcagggc
     1141 gccacggcgg acgatcttca tgcccgtgta ctccttcggg ttgtgcgagg cggtcacgca
     1201 gatgccgccg tccaggccca gctcgtcgac cgtgttgtag accatctccg ttccgaccat
     1261 cccgatgtcg acgacgtcgg cgccgccgtc cgcagcgccg tcgatcgtcg cagcggccat
     1321 cgcgggagcg gcgagccgca tgtcccgccc gacggcgatc acacgaggct cgaagtgctc
     1381 gacataagcc cgtccgatcg cgtaggcgcc ggcctcgtcg agctcggtgg gatagatgcc
     1441 gcgcacgtcg taggccttgc agacctttgg gtccaacacg gtcctgatgc tatcccggcc
     1501 ggcctctgac agcgccacag gcggccctac acgtctgtgc gtggagcgca agctagcggc
     1561 cgtgctgttc gtcgacatcg tcggctcgac cgagctggtc gccggtttgg acccggaggt
     1621 ggcgcgccgt cgcgtggcga gcttcttcga ccgggtctcg gcctgcatca gcgttcacgg
     1681 cgggacggtc gagaagttcg ccggcgacgc cgtcatggcc gccttcggga tcccgcacgc
     1741 gcacgaggac gacgcggagc gggcggcgcg ggccgcgctg gcgatccgcg acgcggttcg
     1801 cgcgctcgag ctcgaagtca ggatcggcat cgaggcggga gaggtcgtga cgggcggcgc
     1861 cgactcgccg tttgccaccg gcggcccgtt caatctcgcc gcgcgcttgc aggcggaggc
     1921 cgagcccggc cagatcctgc tcggtcccgc cgcgcacagt ctgacgcgcg gccgcatgac
     1981 gaccgagccg ctcgggtcgc gggaactgcg cggcttcgca caaccgatcg acgtctggcg
     2041 acttctcggc gcgcccgacg acgcgggacg tcggatgagc gttaccgcgc ccttcatcgg
     2101 gcgtgacgcc gagctggact tgctcgagaa cacgtggacg cgggccgtcc gtgaccggcg
     2161 ggcgcagctc ttcaccatct acggcgagcc cggcgtgggg aagagccggg tcgcagccga
     2221 gttcgtcgac tcggtcgagg gggcgaccgt gctcgtggga cgctgcctcc cgtacggcga
     2281 gggcatcacc tactggccgc tcgccgagat ggtaaagctg gccgccggga tctcggacga
     2341 cgatccggcc gacgaggcga tggagaagct ccgcgcctgc tgcggagaag acgcggtcgc
     2401 ggatctgctc gctcttgccg cgggcgtgct cgaggcagtg tcaggcgagc gaagcgcgca
     2461 ggagatcgcc tgggcggccg aggagtgggc cggcgagctt gcgtccgcac agccactcgt
     2521 gctcggcttc gaggacatcc actgggcgga ggagcccctc ctcgacctga tcgagcatct
     2581 cgcggaccgc gttcgctctg ttcccctgct catcctgtgc ctggcgcggc cggagctgct
     2641 cgacgtgcgc cccaactggg gcggcggtcg gctgcgcgcg accgcgatcg agctcgagcc
     2701 gctcgtctcg gaggagcgcg agacgctcgt tgacgcgctg ctgggcgacc acgagctggc
     2761 acccgacgtc cgccgccggc tggtggagaa gaccgagggc aacccactct tcctcgagga
     2821 aacggtgcgc atgctgctgg aggacggcga gcaggcgccc gagcgcattc ccgacaccgt
     2881 tcaggctctg atcgcggcca ggatcgacgg cctcccacgg gaggcgaaag acgtcctcca
     2941 gcgcggcgcc gtgatcggcc gcgtcttctg ggagggcgcc gtggccgcgc tcgcgcccgg
     3001 ttcggatgat gtcgccgagg ccctcgagga actcgtcgac cgtgaattcc tcacgctcga
     3061 ggcccgctcg tccatctcgg cggaggaagc gtaccgcttc aagcacgtgc tgatccgcga
     3121 ggtggcctac gcggggatcg cgaaggggga ccgcgcggtg ctccacgagg gtttcgccga
     3181 gtggctgaag gaacgggccg tggaggagat gatcgagatc cgcgcctacc atctcgacga
     3241 ggccgcgcgc ctgagcgaag agctcgaagg cagcgtgccg cccgagctgg cggccgaggc
     3301 ggccgctgct ctcgagcgtg cgggacggcg ggccctcgcc agggaggcga atcgaccggc
     3361 gcgcaacctg ctgcgccgcg ccgtcgagct cgagccgaca ttggagcggc ggtacgacgc
     3421 ggccaaggcg gcatggcggc tcgccgatct gcccaccgtc tctgacgaga tggtgcaagt
     3481 gcgccggctc gccgcggccg agggtgaccg gcggatcgaa ggggccgcct tgaccgcgct
     3541 cgcggaggtc gcgctcctcc gcgatgccga tcttccacgc gggcgtgagc tcgtcgagga
     3601 ggccctcgaa ctcctcggtg aggagccgtc cgagaaccga ttcgatgcac tcgagatgcg
     3661 ggcgatggtc ggctggtggc tcggcgacct cgacgagaac gagcgctacg cccggctcgc
     3721 actggacgtc ggtcgagcgc tggggcggcg cgatctcgag gccagtgcgc tggactacct
     3781 cgccggcagc tccatcgtcc gcctcgacct cgagacggga tcggagttgc tggcctcggc
     3841 gatagagctc agtaccgcga gcggcaaccc tgtcgcgcgg gcctggtcgc ttcacacgca
     3901 gtcccggatc tgcgagctcc aaggcgacct cgacgaggcc gagcgtgtgg ccgaggaggc
     3961 gcgtgagctg ttcgctcaag ccgaggtcag ctgggcgctc gggcgcgtgc tgaaccggct
     4021 cggctggatc gcccgctggc gcggcgacct caagacgtca gagcgaaggt tccgcgactc
     4081 gatccagctg atgaagggaa tcgaggatcg tggcacgctt tgtgagagcc agcgaagcct
     4141 cgcagagctc ctggtgcagc tcgggcggct cgaggaagca gagcagctgg cgctggaggc
     4201 ccgcaccact gtcggggggc acgaccagaa ctcgcgagcg acgacgcgca tggcgctcgg
     4261 gatcgtccgc gccgcccagg ggcggtacga ggaggccgag gccctgttac gagaggcgct
     4321 ggaaatcatc gaacacagcg acgcgaagct cgtgcgagcc gaagtgctgg cggcgatgat
     4381 cgccttcctg cacgagcggg ggcgtgctgc cgacgcagcc ggctacgagg cggagctggc
     4441 ggcgctgcgc tcgacgtcgg taccggcgta gaggatctcg cggatcgcct ggctcgacgc
     4501 ctcgtccggg gactccgaga tgaccgtcgc cgggcgatcg aaccgcgcca gtgcgtcgcg
     4561 gagcggctcc gcccggaggg tgcccgcgcc gtacggcaga tgcttcgtct cgtttcggtt
     4621 cgcgaaggcg atgtccgaga agtggatgtg gaacgcggcg ccgtctgcga ggagctcgtc
     4681 gacggcggcg agagcggtcg caaagggttc ctcatccaga taggcgccgt cgctcgtcgc
     4741 gtgcatgtga gcgaagtcga gcaccgggcg cacccagccc gtgcgacgcg ctatctggag
     4801 cacgtcgtcg agcgagccga gatcgcgcac acgacccatc acctcgacac cgaagggaac
     4861 agctcggctc ttctgctcga ggcgctcgcg gagtgtgccg agctgctcga ccacggcgtc
     4921 gagcgcctcc tcacgc
//
LOCUS       DBNGNK010000379         4224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1210010,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000379 DBNGNK010000000
VERSION     DBNGNK010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1210010"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC150V_11245"
     CDS             complement(<1..378)
                     /locus_tag="AC150V_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763535.1"
                     /translation="MRIDFLTPQAALVGLAAALPLLALALAARRADRARAAVGLGSAG
                     LGPYLGPAVALAALGVLVAVAVSQPILQRRDAVLVRTDAAAFVVVDTSRSMLAAAGPD
                     AVTRLVRARRAALQIREKLADVPV"
     gene            complement(380..991)
                     /locus_tag="AC150V_11250"
     CDS             complement(380..991)
                     /locus_tag="AC150V_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763536.1"
                     /translation="MSRRCVPVVVAAVLLSLAAAAGLAAFEAWRWTAHASAEDRRLAR
                     ELVPAGAWAERGGFAADVLGAADDVAFRRAIVQFRRGRGDFFGGEKTIEQVVSSVEAG
                     LGLAAVARGDAPAERRSAALNLLAILTGEVAVFENDSETRIRAAATQLRQAIRLNPAN
                     DAAKANLELLLRLTGGGDTPVEGTGGFGGLGEDAAAGAGGSGY"
     gene            complement(988..1845)
                     /locus_tag="AC150V_11255"
     CDS             complement(988..1845)
                     /locus_tag="AC150V_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR3763537.1"
                     /translation="MKRLVLADARGLARGGRRTTALRVGSALVLAGLLAAVVGLLARG
                     ETGEPPLDEAGRTTLLVIDVSSSIQPQVYEQIGATLDRAMAEGGKFGVVLFSDASYEM
                     LPPGTDAAELDGLRRYFTPLQDVPTGVQTVSVGRTRFAKPPWHESLARGTRISSGLVL
                     ARSVLRREGVANGKVVLVSDLETEFFDIAQTGEIIAAYAAERLPLRVVALSPSPEDKR
                     IFTRLLGDAGSIDVASAPAEGERDAAALPPVPFPLVLAVLGVALGLALAANEHLLARL
                     PLRTRGAER"
     gene            complement(1842..2714)
                     /locus_tag="AC150V_11260"
     CDS             complement(1842..2714)
                     /locus_tag="AC150V_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763538.1"
                     /translation="MRGALSALAIALGAGLLIAAATGRGDDVSLPAVSEVRAQGSLDA
                     DGARFGDPVIGRLDVLLPRDEVDPGSVRLRADFAPFRLVRPPSVERLVSGPAALVRYR
                     FELECLTTACLPSRDGRGLVRPSAAVSFADAGGAPRTLAVAWPAVGTASWHGGAGVDP
                     QWRSGLERLPAYDYLLPPRTTVLLFALVAAALTVAAIAILFPSIRRALPKARPATDRR
                     SLLERALATVRSAAGRDDPAERRRALDLLSRELRTRRRVSEAGDASHLAWSRAEPEGG
                     EMEEIADRVERAGA"
     gene            complement(2711..3631)
                     /locus_tag="AC150V_11265"
     CDS             complement(2711..3631)
                     /locus_tag="AC150V_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR3763539.1"
                     /translation="MSAAPHSVRDAFPLVPRHRLGGLELGPFRSARRGAGSDPAGSRP
                     YEPGDDVRLIDWAASARLSAARQADEFVVTERYAEQAPRIVVLSDRRPGMALYRAPWL
                     SKPAVVATCERLIGDSAFRVRGLVAALHFVGSDPRWSPPTGNPLVWQAREQQDGYTAE
                     PGLLAEGIERIAHSRAMTAGSFLFVLSDFLDPVPEETWRLALARDWDVVPVVIRDPTW
                     EASFPVEVGGLVLPLQDPATGEHSLVRLSKLEAEQRREHHETRRATLLGDLVELGLEP
                     IELGSDDPEAVFGAFLEWASRRLAPKGRAW"
     gene            complement(3638..>4224)
                     /locus_tag="AC150V_11270"
     CDS             complement(3638..>4224)
                     /locus_tag="AC150V_11270"
                     /inference="COORDINATES: protein motif:HMM:NF036831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3763540.1"
                     /translation="GGATRRLPRPFLLLATENPIEHEGTFPLPEAQLDRFFLKTALGY
                     PGLDDERRIVTEQRHGHPLGELQPAVGLEELQQLQDAVEDVYVDRLLVDWLLQLVRGT
                     RALEGVALGASVRATLALERAARAWALINGRPYVVPEDVEQLFLPVVGHRILFTPAYL
                     VELRREGPQAALERFRAACFAAAPRPEPSPVRPV"
BASE COUNT          658 a         1548 c         1499 g          519 t
ORIGIN      
        1 caccggcacg tcggcgagct tctctctgat ctgcagcgcc gcccggcggg cgcgaacgag
       61 acgggtcacg gcatccgggc cggccgccgc cagcatcgag cggctggtgt ccacgacgac
      121 gaaggccgcc gcgtccgtgc gcacgagaac cgcgtcgcgc cgctggagga tcggctgcga
      181 gacggcgacg gcgacgagga ctccgagcgc cgcgagtgcc acggccggcc cgagataagg
      241 ccccaaaccg gcggacccga gccccactgc ggcccgagct ctgtcggcgc gccgcgcggc
      301 gagagcgagc gcgagcaagg gaagcgcagc cgccagaccg acgagcgcag cttgcggcgt
      361 gaggaagtcg atccgcacgt cagtacccgc tcccgccagc cccggcggcc gcatcctcgc
      421 cgaggccgcc gaagcctccg gtgccctcga ccggcgtgtc tccgccgcct gtcagccgca
      481 ggagcagctc caggttcgcc ttggccgcat cgttggcggg attcagacga atggcctgac
      541 gcagctgcgt cgcggccgcg cggatccgcg tctccgagtc gttctcgaag accgcgacct
      601 cacctgtgag gatggccagc aggttcaagg cagccgaccg tcgctcggct ggagcgtcac
      661 cccgggcgac cgctgcgagc ccgagtcccg cctcgaccga cgagaccact tgctcgatcg
      721 tcttttcgcc gccgaagaag tcgccccgcc cgcgccggaa ctggacaatc gcacggcgaa
      781 atgccacgtc gtcggcggcg ccgagaacgt ccgcggcgaa tccgccccgt tccgcccagg
      841 cgccggccgg gacgagctcc cgcgcaagcc gcctgtcctc ggccgatgca tgcgccgtcc
      901 agcgccaggc ttcgaaggcg gccaggccgg ctgccgccgc gagcgacagg aggactgccg
      961 caacgactac cgggacgcag cggcgactca ccgctccgct ccccgggtgc gcaacggcag
     1021 gcgcgcgagt aggtgctcgt tcgccgcgag cgccagccca agggcgacgc cgagcacagc
     1081 cagcacgagc gggaacggga ccggcgggag cgcggcggca tcacgctccc cttcggccgg
     1141 agcagacgct acgtcgatcg agcccgcatc acccaggaga cgtgtgaaga tccgcttgtc
     1201 ctcggggctc ggcgacagcg cgacgacgcg cagcggcagc ctttccgccg cgtacgctgc
     1261 gatgatctcc ccggtctgcg cgatgtcgaa gaactcggtc tcgagatcgc tgacgagtac
     1321 cactttcccg ttcgcgactc cctctcgccg caggacggaa cgcgcgagca cgagtccaga
     1381 cgagatccgc gtcccgcgcg ccagggattc gtgccacggg ggcttggcaa agcgcgtgcg
     1441 cccgacggag accgtctgca cgccggttgg cacgtcctgg agcggagtga agtagcgccg
     1501 gagcccgtcg agctccgcgg cgtcggttcc gggcgggagc atctcgtagg aggcatcgga
     1561 gaagagcacg actccgaact tcccgccctc ggccatcgcc cggtcgagcg tggcgccgat
     1621 ctgctcgtac acctgcggct ggatgctcga cgacacgtcg atgacgagaa gggtggtgcg
     1681 gccggcctcg tcgagtggcg gctcgccggt ctcaccgcgc gccaggagcc cgacgacggc
     1741 cgcgagcagt cccgcaagca cgagcgccga cccgacgcga agggcggtgg tgcgccgccc
     1801 cccgcgcgca agtccccgtg catctgcaag cacgaggcgc ttcacgcccc agccctctcg
     1861 acccgatccg cgatctcttc catctccccg ccctccggct cggctcgcga ccaggcaaga
     1921 tgactcgcgt cgccagcctc cgagacgcgc cgtcgagtac gcagctcgcg cgagagcagg
     1981 tcgagagcac gcctgcgttc tgcggggtcg tcacgcccgg ctgcagaccg aacggtcgcc
     2041 agggcacgct cgagcaagct ccggcggtcg gtggccggtc gcgccttggg aagtgcgcgc
     2101 cggatgctcg gaaagaggat cgcgatcgcc gcgaccgtga gcgcggcggc gacgagcgcg
     2161 aaaaggagca ccgtcgtgcg cggcggcagc aggtagtcgt aggccggaag ccgttcgagc
     2221 cccgagcgcc attgcggatc gacgcccgcg cccccgtgcc aggacgcggt gccaacggca
     2281 ggccacgcca cggcgagggt tcgcggggcg ccgccggcgt cggcgaagct gaccgctgcg
     2341 ctcgggcgca cgagccccct gccgtcccgt gatgggaggc atgcggttgt caggcactcc
     2401 aactcgaagc ggtaccgcac gagcgcggcg gggccgctga cgagacgctc cacactgggg
     2461 gggcggacga gccggaaggg agcgaagtcg gcccgcagcc tcacgctgcc tgggtcgact
     2521 tcgtcccgtg gcaggaggac atcgagtcgc ccgatcacgg gatctccgaa ccgggcgccg
     2581 tccgcatcga ggctgccttg agcacgcact tccgagacgg cgggcagaga aacgtcgtcg
     2641 ccgcgtccgg tcgccgccgc gatcagcagc cccgcgccga gcgcgatcgc gagcgcggag
     2701 agcgcccccc tcaccacgca cgccccttgg gagccagccg tctcgacgcc cattcgagga
     2761 acgcgccgaa gacggcttcg ggatcgtcgc tgccgagctc gatgggttcg aggccgagct
     2821 ccacgaggtc tcccaggagc gtcgcccggc gcgtctcgtg gtgctcgcgc cgctgctccg
     2881 cctcgagctt gctcagtcgc acgagcgagt gctcgcccgt tgcggggtcc tgcagcggca
     2941 gcacgaggcc gccgacctcc acggggaagc tcgcctccca ggtcggatca cgaatcacga
     3001 ccgggacaac gtcccagtcg cgcgcgagtg cgaggcgcca ggtctcctcg ggaaccgggt
     3061 cgaggaagtc cgagagcacg aacagaaagc ttccggccgt catcgcccgg gagtgggcga
     3121 tccgctcgat cccctcggcg agcagccccg gctctgccgt gtagccgtcc tgctgctcgc
     3181 gcgcctgcca cacgagcgga ttcccggtcg gcggcgacca tcgcggatca gagccgacga
     3241 agtgcagcgc ggcgacgagt ccccggactc tgaacgcgct gtcgccgatc agccgctcgc
     3301 acgtcgccac tacggccggc ttggacagcc acggcgcccg atacagcgcc attccgggtc
     3361 ggcggtccga gagtacgacg attcgcggcg cctgctccgc atagcgctcg gtgacgacga
     3421 attcgtccgc ttgacgcgcc gccgagaggc gcgctgacgc cgcccagtcg atcagcctga
     3481 cgtcgtcgcc gggttcgtag ggacgcgagc cggcgggatc ggagcccgcg ccgcgccgcg
     3541 cgctgcggaa cggtcccagc tccaacccgc cgagccggtg ccgcggaaca aggggaaagg
     3601 cgtcgcggac cgaatggggc gccgcgctca ccaggcttca gacgggtcga acgggcgacg
     3661 gctccggcct cggcgcggcc gcgaagcacg cggcacgaaa ccgctcgagc gccgcttgcg
     3721 gcccttcacg acgcagctcg accaggtagg ccggcgtgaa gaggatccgg tgtccgacga
     3781 ccgggaggaa gagctgttcg acgtcctccg ggacgacgta gggccgcccg ttgatgagcg
     3841 cccacgcacg cgcggcccgc tcgagtgcga gcgtcgcgcg gaccgaggcg ccgagcgcga
     3901 cgccctcgag cgcgcgagtg ccgcgaacga gctgcagcag ccagtcgacg agcagccggt
     3961 cgacgtagac gtcctcgacg gcgtcctgga gctgctgtag ctcctccagg ccgaccgcgg
     4021 gctgcagctc gccgagcgga tgcccatggc gctgctcggt cacgatgcgg cgctcgtcgt
     4081 ccagtcccgg atagccgagt gccgtcttca ggaagaagcg gtcgagctgg gcttcgggca
     4141 gcgggaacgt cccctcgtgc tcgatcgggt tctcggtcgc gaggagcagg aacggcctcg
     4201 gcagacgccg cgtcgcgcca ccta
//
LOCUS       DBNGNK010000380         3078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1569477,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000380 DBNGNK010000000
VERSION     DBNGNK010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1569477"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="AC150V_11275"
     CDS             complement(<1..408)
                     /locus_tag="AC150V_11275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763541.1"
                     /translation="MRLADAVSIRSRRRKLRLFLDEMQPGAETTVLDVGVDEIGFGGE
                     GGETGCATHNFFEELYPWPGSITAVGLHEGAGFRDRLPDIRYVQADACDLPFADKSFD
                     LYFSNAVIEHVGGAERQRRLVAEAVRVAHRVFIT"
     gene            616..1320
                     /locus_tag="AC150V_11280"
     CDS             616..1320
                     /locus_tag="AC150V_11280"
                     /EC_number="3.2.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase family protein"
                     /protein_id="HIR3763542.1"
                     /translation="MTIRASDAWALDRAHFMLALDDDTAEFHRRFSRDPLLGPSTRAL
                     VGYRPLRLATVAHAALRALCGQLIEARRARAIERSILRASGSASPTQDELARLAPVEL
                     RRRGLAMSRATALARLYRTVDLERLRHVPTPAVVTRLTREPSVGPWSCGVIALKGLGR
                     YDHGIVGDLALMKLTSSLRDRWVEPWETAELLQPYGEWQGLAGVFLLAGWRRGLVPGA
                     DADRARAARVRSRHAA"
     gene            1350..2174
                     /gene="proC"
                     /locus_tag="AC150V_11285"
     CDS             1350..2174
                     /gene="proC"
                     /locus_tag="AC150V_11285"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR3763543.1"
                     /translation="MAIDPRRIALLGGGKIGGALIAGLLSSGWRAPADIVATARREER
                     IAELSELYAIETTLSNAEAVSGAALIVIAVKPQDIEVLLGEVGGLVSVDQTVLSIAAA
                     IPTAAIERHLAPGVPVVRAMPNTPSTVHEGVAGVCPGAHADDEHLTLAEEALAHLGAV
                     VRVPERQMDAVTAVSGSGPAYFALLAEAMIEAGILLGLSREISTQLVVQTMLGTAKQL
                     RDEKVHPVELREMVTSPGGTTIAAIRELESAGVRAAFLNAIQAAMDRSKELAAGEP"
     gene            2178..2849
                     /gene="pgl"
                     /locus_tag="AC150V_11290"
     CDS             2178..2849
                     /gene="pgl"
                     /locus_tag="AC150V_11290"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR3763544.1"
                     /translation="MDDPLEVRVLDDPVETAAELLAEVARGGGHIALSGGSTPAPAYE
                     QAAELAPDWSQVEVWFGDDRAVAPDDERSNLLLVERHLLSRLERAPAAVHRIHGEIGA
                     AAAAAAYEAELDGRALDLALNGVGPDGHTASLFPSSPGLRERTRQVIAAEPGFEPFVD
                     RVTLTPPAFARVGVLVYLVTGPLKAEPARRAFVEPPDESVPASVVRGLKTLALLDRAA
                     GSQLG"
     gene            complement(2891..>3078)
                     /locus_tag="AC150V_11295"
     CDS             complement(2891..>3078)
                     /locus_tag="AC150V_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763545.1"
                     /translation="CRRAVKAVLRSFEERDAFLEDVAVADTVLVLERLAARRGIHIAL
                     RSSLLPSRPGGTPAHDM"
BASE COUNT          466 a         1069 c         1091 g          452 t
ORIGIN      
        1 cgtgatgaag acgcggtgag cgacgcgcac cgcctcggcc acgagccgtc gctggcgctc
       61 ggcgccgccg acgtgctcga tcaccgcatt cgagaagtag aggtcgaagg acttgtccgc
      121 gaagggcaag tcgcaggcgt cagcctgcac gtagcggatg tcgggaagac ggtcacggaa
      181 gcccgcaccc tcgtggagcc cgacggccgt gatcgagccg ggccacgggt acagctcctc
      241 gaagaagttg tgcgtcgcac aacccgtctc gcctccctcg cccccgaagc cgatctcgtc
      301 gacaccgacg tccagcacgg tggtctcggc gccgggctgc atctcgtcga ggaagaggcg
      361 cagcttgcgc cggcgtgagc ggatcgacac ggcgtctgcg agcctcactc gggcgacggt
      421 accggcccga caagcgccct gcacgaagcc gacagggacg tttccggcgg gagcagtagg
      481 ctgccccgcg tggctgaagc gaccgtccgc ccccggggcc cttatcggct ccgcctgatg
      541 cgccaccgcg gacgctggtc cgcctcactg cccggcggcc gatcggcctc cgcctggcag
      601 agcactgacg gggcggtgac gatacgtgcc agcgacgcgt gggctctcga ccgcgcacac
      661 ttcatgctgg cgctcgacga cgacacagcc gagttccatc ggcgcttctc gcgcgatcca
      721 ctgctcgggc cgtcgactcg cgccctcgtc ggttaccgac cgctgcgcct cgcaacggtc
      781 gcgcacgcgg cgctgcgggc gctctgcggc cagctgatcg aggcacgacg cgcacgtgcg
      841 atcgagaggt cgatcctccg tgcgtcggga tccgcgagcc cgacgcagga cgagctcgca
      901 cggctcgcgc cagtcgagct ccgacgccga ggtctggcga tgagccgtgc aactgcgctg
      961 gcgcggctgt accgcacagt cgatctcgag cggcttcgcc acgtaccgac gccggcagtc
     1021 gtgacgagac tgacacgcga gccgagcgtc ggcccatggt cgtgcggcgt gatcgcgctc
     1081 aagggactcg gccgctacga ccacggcatc gtcggcgacc tggctctgat gaagctgacc
     1141 tcgagcctgc gcgatcgctg ggtggagcca tgggagacgg ctgagctgct ccagccgtac
     1201 ggcgagtggc aggggctcgc cggtgtgttt ctcctggcgg gctggcggcg cggcctcgtc
     1261 cctggcgccg acgccgatcg agcccgcgcc gcccgcgtgc gttcacgcca cgcggcataa
     1321 gggcggcagc gaggccgtag actccgatcg tggctatcga tccccgcaga atcgcgctcc
     1381 tcggaggcgg caagatcggc ggcgcgctga tcgctggcct actgagctcg ggctggcggg
     1441 cgccggcgga catcgtcgcg acggctcggc gagaggagag aatcgcggag ctgagcgagc
     1501 tatacgcgat cgagacgacg ctctcgaacg ccgaggcggt gtcgggcgcg gcgttgatcg
     1561 tgatcgcagt caagccgcag gacatcgagg tcttgctggg cgaggtcggc ggactggtct
     1621 cggtcgacca gacggttctc tcgatcgccg ccgcaattcc gacggcggcg atcgaacggc
     1681 acttggcacc gggagtgccc gtcgtgcggg cgatgccaaa cacaccgtcg accgtccacg
     1741 agggcgtggc cggcgtgtgc cccggcgccc atgccgacga cgagcacctc actctggccg
     1801 aggaagcgct cgcccacctc ggcgctgtgg ttcgcgtccc cgagcggcag atggacgccg
     1861 tgacagcggt gtcgggatcc ggacctgcgt atttcgcgtt gctcgccgaa gcgatgatcg
     1921 aggccggcat tctgctcgga ctctcgcgcg agatctccac ccagctcgtc gttcagacga
     1981 tgctcggtac agcgaagcag ctgcgcgacg agaaggttca cccggtcgag ctcagggaga
     2041 tggtgacgtc gcccggtgga acgacgatcg ccgccatccg tgagctcgag tccgccggcg
     2101 ttcgggcggc gttcctgaac gcgattcagg cggcgatgga ccgatccaag gagctcgccg
     2161 cgggcgagcc ctagacggtg gacgatccgc tcgaggttcg cgtcctcgac gatcccgtcg
     2221 agacggccgc cgagctgctc gcagaggtcg cgcggggcgg gggccacatc gccttgagcg
     2281 gcggctcgac cccggcaccg gcgtacgagc aggccgccga gctggctccg gactggtcgc
     2341 aggtcgaagt ctggttcggc gacgatcgtg cggtcgcccc tgacgacgaa cgctcgaacc
     2401 tcttgctcgt cgagcggcat ctgctcagca gactggagcg cgctcccgcg gccgttcatc
     2461 ggatacatgg tgagatcggg gcggcagcgg ccgccgccgc ctacgaggca gagctcgacg
     2521 gcagagcgct cgatctcgcc ctgaacggcg tgggccccga cgggcacaca gcctcgctct
     2581 ttccgtcctc gccggggctc agggagcgca cgcgacaagt gatagcggcc gaacctggct
     2641 tcgagccgtt cgtcgaccgc gtcacgttga cgccgcccgc attcgcgcgc gtcggcgtgc
     2701 tcgtctatct cgtcacaggc ccactgaagg cagagccggc ccgccgggcc ttcgtcgagc
     2761 cacccgacga atcggttccg gcgagcgtgg tgcgtggtct gaagacgctt gccttgctcg
     2821 atcgagccgc cggctcacag ctcggctagc aggaggccgg cgccgcccgc cggcctccga
     2881 cgccgaatcc tcacatatcg tgggccggcg tgccgcccgg acgggacggc aggagcgacg
     2941 accgcagagc gatgtggatt ccccgtctcg ccgccagtcg ctcgaggacg agcacggtgt
     3001 cggcgacggc cacgtcttcg aggaatgcgt cgcgctcctc gaacgagcgg agaacggctt
     3061 tgacggcccg gcggcacg
//
LOCUS       DBNGNK010000381         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1497570,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000381 DBNGNK010000000
VERSION     DBNGNK010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1497570"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC150V_11300"
     CDS             complement(<1..632)
                     /locus_tag="AC150V_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR3763546.1"
                     /translation="METWTRFVLRHRWLVIVGTAVVFVAGGWAAQQLSELLTNRFTLP
                     GTDTERAEKILEGEFGQKSAGSFTLVARTDGDAESIVPAVEDAAVRAADEVPSGRFVG
                     VFPLSDTVVSAQIVSELDPADAKGYTDDMRRAIGTIDGADVFVTGQAATEFDLDPVFE
                     EDLQKGEFMIALPIALILLLFTFGTLAFLIPFVFAAVTIPTTLGLVWVFA"
     gene            complement(702..848)
                     /locus_tag="AC150V_11305"
     CDS             complement(702..848)
                     /locus_tag="AC150V_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763547.1"
                     /translation="MHRLKQALAVAGLALGALLYLWVAAVRAVPGVKRRKRARRAARA
                     RPNG"
     gene            968..1462
                     /locus_tag="AC150V_11310"
     CDS             968..1462
                     /locus_tag="AC150V_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763548.1"
                     /translation="MAKTTGDKLADSFENLKPYIERALTDDDFRNNVRTALTAAGAVY
                     GDVSGKNGVSAATKLAGDKKTQQNLRRAFEELNEAGYRLKGKKKSKKGKKTVLLVGFI
                     VGLFYNPWTGPQTRQWLLDKIAGGDDLQPLESWDTGETAASFEATDESAGSAADTAKA
                     DGDS"
     gene            complement(1473..>2441)
                     /locus_tag="AC150V_11315"
     CDS             complement(1473..>2441)
                     /locus_tag="AC150V_11315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR3763549.1"
                     /translation="GLPAGSEIVIGAVEATAEAELVSEAPQPPAKPVAPTSPTSGAPG
                     ATGTGPAGAAGTVPPGPTAPATPKQPTPPPIPPTPVDLNEPGRSRAGGAPTSLVRPVP
                     AGVVSRLSAGGYVFPVFGPASFGDSFGAPRGAYVGGWHHGEDIFAPAGAPILAVASGT
                     IFSVGWNQYGGWRLWLRDQLGNQFYYAHLSAYSPLAVDGREVKAGDVLGFMGTTGDAE
                     FSPPHLHFEIHPVNLLALDYDGVVAPYPYLIAWRRAEDVSFDAGRKYLPDDGPARGRN
                     VAPQVGAVLLQAADISSASGLEPGALGRTLDGGGAVAPTVAVPSAG"
BASE COUNT          447 a          812 c          873 g          309 t
ORIGIN      
        1 ttggcaaaca cccacaccag cccgagggtg gtcgggatcg tgacggcggc gaacacgaag
       61 gggatgagaa acgcgagcgt gccgaaggtg aacagcagca ggatcagcgc tatcggcagg
      121 gcgatcatga actcgccctt ctgcaggtcc tcctcgaaca cagggtcgag atcgaactcg
      181 gtcgccgcct gcccggtgac gaacacgtcc gctccgtcga tcgttccgat cgcccgccgc
      241 atgtcgtctg tgtagccctt cgcgtcggca gggtcgagct ccgacacgat ctgagcgctg
      301 acgacggtgt ccgagagcgg aaacacgccc acgaagcggc cggacggcac ctcgtcggcc
      361 gcacgcacag ctgcgtcctc gacagccggc acgatcgact cggcgtctcc gtcggtgcgg
      421 gcgaccaggg tgaacgaacc ggccgacttc tggccgaact cgccctcgag gatcttctct
      481 gcccgctccg tatcggttcc gggaagcgtg aaccggttgg tcagcagctc ggagagctgt
      541 tgcgccgccc agccacccgc cacgaagacg accgcggtgc cgacgatgac gagccaccgg
      601 tggcgaagta cgaaacgagt ccaggtttcc atgcgacggc gaggcggacg ggactcagct
      661 caaggtaaca acaggcccga gccctccggt gcggtagcgc ctcagccgtt gggccgggcg
      721 cgggccgccc ggcgggctcg cttgcgccgc ttcacaccgg gaacggcgcg aacggcggca
      781 acccagagat agagaagcgc gccgagcgcg agcccggcga ccgcgagagc ctgcttgagg
      841 cggtgcacgc gggcatccta atcgcctcgc ccacagccct cgagcggccg cgtcaacgtg
      901 ccgctgtgcg caatcatccg gtaatacttc cggggtcagg gaatttcaga ggaaaggacg
      961 gccgggaatg gccaagacca caggtgacaa actcgccgac agcttcgaga acctgaagcc
     1021 ctacatcgag cgggcgctca ccgacgacga cttccgtaac aacgtgcgca cggcactcac
     1081 ggcggccggc gcggtgtacg gcgacgtctc gggcaagaac ggggtgtcgg ccgcgacgaa
     1141 gctcgccggt gacaagaaga cgcaacagaa cctgcgccgc gcgttcgaag agctcaacga
     1201 ggccggctac cggctgaagg gcaagaagaa gtcgaagaag gggaagaaga ccgtcctcct
     1261 cgtcggtttc atcgtgggac tcttctacaa cccgtggacc gggccccaga cccgtcagtg
     1321 gctgctggac aagatcgcag gcggagacga cctccagccg ctcgagtcat gggacacggg
     1381 cgagacggcg gcgtcgttcg aggccaccga cgagagcgca gggtcagcag ccgacacggc
     1441 gaaggccgac ggcgacagct gacgcattcc gcctagccgg ccgacggaac agccacggtc
     1501 ggagcgaccg caccaccacc gtcgagtgtg cgcccgagcg ctcccggctc gagcccgctc
     1561 gcgctggaga tgtctgcagc ttggagcagg acggcgccga cctggggcgc gacgttccgt
     1621 ccgcgcgcgg ggccgtcgtc gggcaggtac ttgcgcccgg catcgaacga gacgtcttcg
     1681 gctcgtcgcc aggcgatgag gtacgggtag ggggcgacga cgccgtcgta gtccagtgcg
     1741 aggagattga ccggatggat ctcgaagtgg aggtgcggcg gcgagaactc ggcatccccg
     1801 gtcgtcccca tgaagcccag gacgtcaccc gccttgacct cgcgcccgtc gaccgccagc
     1861 ggcgagtagg ccgagagatg cgcgtagtag aactggtttc cgagctggtc ccgtagccac
     1921 agacgccacc cgccgtactg gttccagccg accgagaaga tcgtccctga ggcgacggcg
     1981 agaatcggag cgcccgccgg agcgaagatg tcttcgccgt ggtgccagcc gccgacgtag
     2041 gcgccgcggg gggccccgaa ggagtcccca aacgaagcgg gcccgaagac gggaaagacg
     2101 tatccgccgg cagagagccg cgagacgacg ccggctggga ccgggcggac gagcgacgtc
     2161 ggcgctcctc ccgcccggga gcggcccggc tcgttgagat cgacgggcgt ggggggaatc
     2221 ggcggtggag tgggctgctt cggagtcgcg ggcgccgtcg ggcccggcgg caccgtcccg
     2281 gcggcacccg caggacctgt gccggtcgcg ccgggagcgc cggacgtcgg cgaggtcggc
     2341 gcaacgggct tggcgggcgg ctgcggagcc tcggacacga gctcggcctc ggcggttgcc
     2401 tcgacggcgc cgatcacgat ctcgctaccg gcgggaagcc c
//
LOCUS       DBNGNK010000382         8454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_854749,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000382 DBNGNK010000000
VERSION     DBNGNK010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8454
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_854749"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..716
                     /gene="pdxH"
                     /locus_tag="AC150V_11320"
     CDS             120..716
                     /gene="pdxH"
                     /locus_tag="AC150V_11320"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR3763550.1"
                     /translation="MDAADLDPDPYRQFTAWLGDAGASGIRWPEAMTLATADATGVPS
                     ARVVLLRGHDERGLTFYTNRDSRKGDELAENPQAAVVFYWDVLDRQVRAEGRVEALSD
                     TDSAAYFQTRPRGSRIAAWASPQSRPLADRDELEKLFADAEARFEGEVDVPLPPFWGG
                     YRLVPVVFEFWQGGAHRLHDRIRYERAGGRWSLIRLGP"
     gene            complement(721..1830)
                     /locus_tag="AC150V_11325"
     CDS             complement(721..1830)
                     /locus_tag="AC150V_11325"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR3763551.1"
                     /translation="MAFLLLVAGVAAGARFGMGDRSSAPKQAAPPAKAAAGAAIAAAP
                     QPGLTPLPVEIRGVHVTGPLASLPGRLKSYVGLKRFGLNTIQLDIKDEGGEVGFVPAD
                     VPLASKAGAARSYYDPKQVASMAKKNGVYLVGRVVTFQDPALARARPDLAIRRPDGSV
                     WTTYGGLGWVNPYDRRVWAYSVSIAAAAARAGFDEIMFDYVRFPSDGDIRSATYPGKT
                     SQPRGRLIADFVAYARKKLKPYGVRISTALFGLAATRDLGIGQVPRLISKSTDTIYPM
                     SYPSLYSDGELGVDDHEATPGETVFRTLSHYRKQLRGRNVQLVSWIQDFSGFTPVQVQ
                     AQIDAARLQGVKGFLLWNADGEYTTQALAPPREIP"
     gene            1997..2566
                     /gene="dcd"
                     /locus_tag="AC150V_11330"
     CDS             1997..2566
                     /gene="dcd"
                     /locus_tag="AC150V_11330"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004022432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR3763552.1"
                     /translation="MVLSDGAIRREIGSGRIRIEPYDPTLMQPSSLDVRVDRYFRVFR
                     NSRYPYIDVRQAQEELTELVEADDDPFILHPGEFVLGSTLERIGLADDLVARLEGKSS
                     LGRLGLLIHSTAGFIDPGWDGHVTLELSNVANLPITIYPGMRIGQLSFMRLSEAAEQP
                     YGSDQLGSKYQGQRGPTPSRYYRNFESGE"
     gene            2563..3453
                     /locus_tag="AC150V_11335"
     CDS             2563..3453
                     /locus_tag="AC150V_11335"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR3763553.1"
                     /translation="MIVLVTGAAGFVGRSTVRELSKRGHRVRAMVRDLAGATALDDID
                     CELVRGDVTDPESLAAAAGGCDVVVNLVAILTGKPADFERVIVGGTANTLAAAAEGGA
                     SRFVQMSALGTSETTKNLVPYYGSKWDAEQTVAASPLPHTILRPSFVFGPDGGALGTF
                     LRIARLAPVTPIVGPGTQRIQPIWIEDLASIIASCVEGATDGLVELGGPDVVTWNEFW
                     QLLKDELGSSRPSLHVPFWFLRPQALLLERLPNPPLTRDQLKMLDAGDNVADPNDAER
                     FEIELVPLSEQLRRTIELSG"
     gene            complement(3804..4391)
                     /locus_tag="AC150V_11340"
     CDS             complement(3804..4391)
                     /locus_tag="AC150V_11340"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR3763554.1"
                     /translation="MLDDRVRAVLARLEAENAHEREEGVARELRSRQVSPTTGRFLFA
                     LVAANAGCEVLEIGGSRGCSAIWLAAGARILGGRVLSLEHDPRKCEAWRRNVGEAGLS
                     EWAELREGDALRELDRIEDGFDLVFLDAEKEDYEALFALVRPKLEAGGLIVADNVLSH
                     ADTLGAYSSARQADASLVSVTIPLDRGLEVTAVLR"
     gene            complement(4421..4738)
                     /locus_tag="AC150V_11345"
     CDS             complement(4421..4738)
                     /locus_tag="AC150V_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR3763555.1"
                     /translation="MPEHEVAPLSELEPGSITIVRVDGRDIGVYNCGGDLYAIEDRCS
                     HDDGPLCEGEWEPAECVAICPRHGARFDIRTGAALTLPAYIPVDTFPVRVRGEDGVVV
                     VDV"
     gene            complement(4747..5181)
                     /gene="sufU"
                     /locus_tag="AC150V_11350"
     CDS             complement(4747..5181)
                     /gene="sufU"
                     /locus_tag="AC150V_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR3763556.1"
                     /translation="MSEFEQLYRELILDHYKSPRNHGLLEPADASAEGENPLCGDEII
                     VSVRFGESGRIAEIGFDGRGCAISQAATSMLTDIVKGRTAEEVAAMPKDELLDEIGIP
                     LTPVRLKCALLGLGVLKVALHKAKGTPLPEEWGTASNEITVN"
     gene            complement(5178..6422)
                     /locus_tag="AC150V_11355"
     CDS             complement(5178..6422)
                     /locus_tag="AC150V_11355"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIR3763557.1"
                     /translation="MSVTSATTLDARQLRADFAVFEELVGGKPIAYLDSASSTQKPRQ
                     VLDRMRRFYEHEYANVHRGVYRLAERATEGYEGARRTVAAFVNAPTEREVVFTRSATE
                     ALNLVAYAWGLDNLGPDDVVVVTELEHHANFVPWQYIASRTGARFRHIPIDDVGELQL
                     EALGAIEREGRVKVVATGLVSNSLGTINPVERLAAWAHERDAIMVVDAAQAAPHRAVD
                     VQALGCDFLAFSSHKLCGPTGAGALWGRRELLEEMSPFNLGGEMIRSVALDRTSFNEI
                     PHKFEAGTPAIAEAVGFGAAIDYISGIGLDAIEQHEHALLEQALGQFGELDFVTVYGP
                     PAERRAGIVSFNVDGVHPHDVAQILDWSGIAVRAGHHCTQPLMTRLGVAATTRASFYL
                     YSLPEEVDRLVEGLHKVKRSLG"
     gene            complement(6453..>8454)
                     /locus_tag="AC150V_11360"
     CDS             complement(6453..>8454)
                     /locus_tag="AC150V_11360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR3763558.1"
                     /translation="ESDPVRRDVGEGVLSGSFVVEGAGSYEVTAVGPDSYAERVAGVA
                     KEFRHPRSPLEQALNRLLYLLVAIMVPLGALLIAALIQHEIETRQAVETAVAGIVTLV
                     PEGLILLASLTFAAAAVRMARQGALAQQLNAVESLASVDVVCLDKTGTLTESEPRLVA
                     LHPAPGVDEQSVRDAFGRYAAASEDRNATLDAIDAALGAVAVPPAGSVPFSSARRWSA
                     LELAGTRYVLGAPDVVSPALLSDLVVAEQDKGRRVVAIGTTIAALPDDPTGAPPELTP
                     LGIAVLAEGIRANARETVSYLLDEGIELKVLSGDAPETVGSIATDVGLPLRISPKDGR
                     ALPEGDFELAELARMVTVIGRIDPEGKRRVVESLRDQGFYVAMVGDGINDVPALKASR
                     LAVAQGSGTQMAKSVSDVVLLSGDFGTLPQMISQGRQILRNVQRVSKLFVTKSVFAAF
                     LILSIGMTAEEYPLLPRHLTLAAALAVGIPAFFLALAPSHGPWRIEGFMRDVWRFAVP
                     AGVAAGMGVLVSYLVSKNVFDLGLPEARTAATTVLTVIGLYLIVVLEATTDRRSAWVG
                     TLVSVLLAVYVAVVFIPFTRHFFDLAVPTLGGLLAILLGVIVAVGFLVLTSARYVPVP
                     VRAREGLRARRAQEAPYAEPAPGATDAHTEGADEGTPGGQPV"
BASE COUNT         1372 a         2923 c         2886 g         1273 t
ORIGIN      
        1 gagcagctcc cgcgagcgcg gtcccgggat ctcggtccgg atctcgatgg cgcgcagggc
       61 agacaccgcg ttgagcgtag ctggaagggc tgaagaactc gtaggcttgc gcccggaaca
      121 tggacgccgc cgacctcgac ccggatccct accgtcagtt caccgcctgg ctgggcgacg
      181 ccggggcttc gggcatccgc tggcccgagg cgatgacgct cgcgaccgcc gacgcgacgg
      241 gcgtaccgtc ggcgcgagtc gtcctcctcc gcggccacga cgagcgcggc ctcacgttct
      301 acacgaaccg cgacagccgc aagggcgacg agctggccga gaacccgcaa gcggccgtcg
      361 tcttctactg ggacgttctc gaccggcagg tgcgggcgga gggacgcgtc gaggcgctta
      421 gcgacaccga ctcggctgcc tacttccaaa cccgcccaag ggggagccgc atcgccgcct
      481 gggcgtcgcc ccagtctcga cccctcgccg accgagatga gctggagaag ctgttcgccg
      541 acgcggaggc acgcttcgag ggcgaagtcg atgtgcctct accgcccttc tggggcggct
      601 accgactcgt cccggtcgtg ttcgagttct ggcagggcgg agcgcaccgc ctgcacgatc
      661 ggatccgcta cgagcgagcc ggcggccgct ggtcgctgat acgactcggg ccgtagcccg
      721 ctacgggatc tcgcgcggtg gcgccagcgc ctgcgtggtg tactcgccgt cggcgttcca
      781 gagcaggaac cccttgaccc cctggagccg ggcggcgtcg atctgcgcct gcacctggac
      841 gggcgtgaag cccgagaagt cctgaatcca cgagacgagc tgcacgttcc gtccgcggag
      901 ctgcttgcga tagtgcgaga gcgtccggaa gaccgtctcg cccggggtgg cctcgtggtc
      961 gtcgacgccc agctcgccgt ccgagtacag cgagggatag gacatcgggt agatcgtgtc
     1021 cgtgctcttc gagatcaggc gcggcacctg tccgattccg aggtcacgcg tcgccgcgag
     1081 tccgaacagc gccgtcgaga ttcgaactcc gtacggcttg agcttcttcc gcgcgtacgc
     1141 gacgaagtcc gcgatcagcc tcccgcgagg ctgagaggtc ttgcccgggt aggtcgcgga
     1201 gcggatgtcc ccgtcggacg gaaagcggac gtagtcgaac atgatctcgt cgaatccggc
     1261 tcgagcagcg gcagcggcga tcgagaccga gtaggcccac actcgacggt cgtagggatt
     1321 cacccagccg agccccccgt acgtcgtcca cacggatccg tcgggccgcc tgatcgcgag
     1381 gtcgggccgt gcccgcgcca gcgccggatc ctggaaggtc acgacccggc cgaccaggta
     1441 cacgccgttc ttcttggcca tgctcgcgac ctgcttcggg tcgtagtacg accgcgccgc
     1501 gccggccttc gaggcgagtg ggacgtcggc gggcacgaac ccgacttcgc cgccctcgtc
     1561 cttgatgtcg agctggatcg tgttcaagcc gaagcgcttc aagcccacgt agctcttgag
     1621 acgccccggc agggaggcga gcgggccggt gacgtggacg ccgcggatct cgacgggaag
     1681 cggcgtgagg ccgggctgcg gcgccgcggc gatggccgcg cccgctgccg ccttggcggg
     1741 tggagcagcc tgcttcggcg cggacgagcg atcgcccatt ccgaaccggg caccggcagc
     1801 gacgcccgcc accaggagca gaaacgccaa cagaactgct gcgcgacgac gccggcgacg
     1861 acgccggatg gcttcccgac gcgagcgaaa acgctcgttc gggtcgattg gcccatagag
     1921 catggacacc cggccccgga gcgtaccgca ttggagcctg ctgaaggcgc ggtgcgacac
     1981 acgtacgata cgggcgatgg tgctctcgga cggcgcaatc aggcgggaaa tcggctctgg
     2041 ccgcatccgc atcgagccct acgaccccac gttgatgcag ccgtccagcc tcgatgttcg
     2101 cgtcgatcgc tactttcgcg tgtttcgcaa ctcccgctac ccctacatcg acgtgcgcca
     2161 ggcgcaggaa gagctgaccg agctggtcga ggcggacgac gaccccttta tcctgcatcc
     2221 cggtgagttc gtcttgggct cgacgctcga gcgcatcggg cttgccgacg acctcgtagc
     2281 ccgcctcgag gggaagagct cgctgggtcg cctcggcctc ctgatccact cgactgcggg
     2341 gttcatcgac cccggctggg acggccacgt gacgctggag ctctcgaacg tggcgaacct
     2401 cccgattacg atctaccccg ggatgcgtat cggccagctg tcgttcatga ggctctcgga
     2461 ggcggcggag cagccgtacg gctccgacca gctcggctcg aagtaccagg ggcagcgcgg
     2521 accgaccccg agccgctact accggaactt cgagagcggc gaatgatcgt cctcgtgacc
     2581 ggcgcggcgg gcttcgtggg ccggagtacg gtgcgagagc tctccaaacg cggacatcgc
     2641 gtgcgggcga tggtgcgcga cctcgccgga gcgacggcgc tcgacgacat cgactgcgag
     2701 ctggtgcgcg gcgacgtcac cgacccggag agcctggcgg ccgcggcggg cgggtgcgac
     2761 gtcgtcgtca acctcgtcgc cattctcacc ggcaagccgg cggacttcga gcgcgtgatc
     2821 gtcggaggca cggccaacac gctggcggca gccgccgagg gcggggcgag ccgcttcgtc
     2881 cagatgagcg cactcggcac cagcgagacg acgaagaacc tcgtgcccta ctacgggtcg
     2941 aagtgggacg cggaacagac ggtggcagcg tcgccgctgc cacacacgat cctacgcccg
     3001 agcttcgtgt tcggccccga cggcggcgcg ctcgggacgt tcctccgcat tgcccgtctg
     3061 gcgccggtca cgccgatcgt cggcccggga acgcagagga tccagcccat ctggatcgag
     3121 gatctcgctt cgatcatcgc ctcctgcgtg gagggtgcaa ccgacggatt ggtggagctc
     3181 ggcggccccg acgtggtgac gtggaacgag ttctggcagc tcctcaagga cgagctcgga
     3241 tcgagtcgcc cctcgctcca tgttcctttc tggttcctgc ggccgcaagc cctgctcctc
     3301 gagcgactac cgaacccgcc cttgacgcgc gaccagctga agatgctcga cgcgggcgac
     3361 aacgtggcgg acccgaacga cgccgagcga ttcgagatcg agctcgtacc gctctccgag
     3421 cagctccggc ggacgatcga gctctcgggc tagcggctcg caggcgcccg acgagaactc
     3481 gcctcggacg agtcgtgctc gcaaaagaag atccggaaag agaaaggggc cgcccctgcg
     3541 gacggccccg tttccggtag ccagcgttgg agtggtagag gcgcttcctg cccgctgccc
     3601 gaaggtggcc ggcggcgcga aaacgcgagt cccctacgcg tggctatggc ggttccccag
     3661 tgggtctcgg ggacacgcct ctacccgctg gtacctccac tgccggtact acaggaatct
     3721 gtcactaccg gaccacctcc tttccgcggt acgcgcactg tagccgttcg tccggccgtc
     3781 gtgaagtgtt cagcgaatgc gcgtcagcga agcaccgcgg tgacctccag cccgcggtcg
     3841 agcgggatcg tgacgctcac gagcgaggcg tccgcctggc gtgcgctcga gtacgcgccg
     3901 agcgtgtccg cgtgagagag cacgttgtcg gcgacgatta gcccgcccgc ctcgagcttg
     3961 ggccgcacga gcgcgaagag cgcctcgtag tcctccttct cggcgtcgag gaagacgaga
     4021 tcgaagccgt cttcgatcct gtcgagctca cggagggcat cgccttcgcg gagctctgcc
     4081 cattcagaaa gccccgcttc gccgacattg cgccgccacg cctcgcactt gcgcggatcg
     4141 tgctccaggg agaggacacg gccaccgaga atccgcgcgc ctgccgccag ccagatcgcg
     4201 gagcagcccc gcgacccgcc gatctccagc acctcgcacc cggcgttcgc cgccacgagc
     4261 gcgaatagga agcgcccggt cgtcgggctg acctggcggg aacgaagctc tctcgccacg
     4321 ccctcctccc gctcgtgcgc gttctcggcc tcgagacggg cgagcacggc acgaacacgg
     4381 tcgtcgagca cagcgcgacc ctagcgagtc ccgctctcgc tcagacgtcg acgacgacga
     4441 cgccgtcctc gccccgcacg cgcacgggaa aggtgtcgac aggaatgtaa gcgggcagcg
     4501 tcaaggcagc tccggtgcgg atgtcgaagc gcgcaccgtg acgcgggcag atcgcgacgc
     4561 attcggccgg ctcccactca ccctcgcaca gcggtccgtc gtcgtgcgag cagcgatcct
     4621 cgatcgcgta caggtcgccg ccgcagttgt agacaccgat gtcccggccg tcgacgcgaa
     4681 cgatcgtgat ggaaccgggc tccagctctg acaggggcgc aacctcgtgc tcgggcacag
     4741 tgtcgcctag ttgacggtga tctcgttgga cgccgtgccc cactcctccg gcaagggcgt
     4801 ccccttcgcc ttgtgcagcg cgaccttgag cacgcccagg cccagcagcg cgcacttgag
     4861 gcgcaccggg gtgagcggga tcccgatctc gtcgaggagc tcgtccttcg gcatcgctgc
     4921 cacctcctcc gccgtgcggc ccttgacgat gtcggtcagc atcgacgtcg cggcctggct
     4981 gatggcgcag ccgcgaccgt cgaagccgat ctccgcgatc cgaccgctct cgccgaagcg
     5041 caccgagacg ataatctcgt cgccgcagag cgggttctcg ccctcggccg aagcgtcggc
     5101 cggttcgagc agcccgtggt ttcgcgggct cttgtagtga tcgaggatca gctcccggta
     5161 gagctgctcg aactcactca cccaagcgac ctcttcacct tgtgcaggcc ctcgaccagc
     5221 ctgtcgacct cctccggaag ggagtagagg tagaagctcg cccgcgtcgt ggcggcgacg
     5281 ccgagcctcg tcatcagggg ctgcgtgcag tggtgaccgg cccgaaccgc gatcccgctc
     5341 cagtcgagga tctgcgccac gtcgtgcgga tgcactccgt cgacgttgaa cgagacgatc
     5401 cccgcacgcc gctcggccgg cggcccgtac accgtgacga agtccagctc gccgaactgc
     5461 ccgagcgcct gctcgagcag cgcgtgctcg tgctgctcga tcgcgtcgag cccgatgccg
     5521 gagatgtagt cgatcgccgc gccgaagccg accgcctcgg cgatagcggg tgtgcccgct
     5581 tcgaacttgt gggggatctc gttgaagctc gtgcggtcga gcgccaccga gcggatcatc
     5641 tctccgccga ggttgaacgg cgacatctcc tcgagcaatt cccgtcgtcc ccagagagca
     5701 cccgcgccgg taggcccgca gagcttgtgc gacgagaagg cgaggaagtc gcagcccaga
     5761 gcctgaacgt cgacggcgcg gtgcggagcc gcctgtgctg catcgacgac catgatcgcg
     5821 tcacgctcgt gggcccaggc ggcgaggcgc tcgacggggt tgatcgtgcc gagcgagttc
     5881 gagacgaggc ccgtggccac gaccttgaca cgaccctccc gctcgatcgc gccaagggcc
     5941 tcgagctgca gctcgccgac gtcgtcgatc gggatgtggc ggaaacgtgc tcccgtcctg
     6001 ctggcgatgt actgccacgg aacgaagttg gcgtggtgct cgagctccgt gacgacgacg
     6061 acgtcgtcgg ggccgagatt gtccagcccc caggcgtagg cgacgaggtt gagagcctcg
     6121 gtcgcgctgc gggtgaagac cacctcgcgc tcggtgggag cgttgacgaa cgcagcgacc
     6181 gtccgtcgcg cgccttcata gccctcggtc gcccgctccg cgagtcggta gacgccgcga
     6241 tggacgttcg cgtactcgtg ctcgtagaac cggcgcatcc gatcgagcac ctggcggggc
     6301 ttctgggtcg aggacgccga gtcgaggtac gcgatcggct tgccgcccac gagctcctcg
     6361 aagaccgcga agtcggctcg aagctgacgc gcgtcgaggg tggtggcgct ggttacggac
     6421 acggtttcat cgtaccggcg cccggtcgcg gctcagaccg gctggccgcc cggagttccc
     6481 tcgtctgctc cctcggtgtg tgcgtctgtc gcccccggtg cgggttccgc gtacggcgcc
     6541 tcctgcgcgc ggcgggcccg caggccctcg cgcgccctga ccggcacagg tacgtagcgc
     6601 gcagacgtca ggacgaggaa gccgacagcg acgatcacgc cgagcaggat cgcgagcagc
     6661 ccgccgaggg tgggcactgc gaggtcgaag aagtgccgtg tgaacgggat gaagacgacc
     6721 gcgacgtaaa cggcgagcag aacggagacg agggttccca cccaggcact gcgccggtcg
     6781 gttgtcgcct cgagcacaac gatcaagtaa aggccgatga cggtcaggac tgtcgtcgcc
     6841 gccgtccggg cttcgggcag accgagatcg aacacgttct tcgagacgag gtagctgacc
     6901 aacacgccca tccccgccgc gactccggcc ggcacggcga aacgccacac gtcacgcatg
     6961 aagccttcga tccgccatgg cccgtgactc ggcgccagtg ccaggaagaa agccgggatg
     7021 ccgacggcaa gcgcggccgc gagcgtgagg tggcgcggca agagcgggta ctcctccgcc
     7081 gtcatgccga tcgacaagat caggaacgcg gcgaacacgg acttcgtgac gaagagcttc
     7141 gagacccgct gaacgttgcg caggatctgg cggccctgcg agatcatctg cgggagggtg
     7201 ccgaagtcgc cggagagcag caccacgtcc gagaccgact tggccatctg cgtgccggat
     7261 ccctgggcga ccgccagtcg cgaggccttc agcgcgggaa cgtcgttgat cccgtctccg
     7321 accatcgcga cgtagaaccc ctggtcgcgc aacgactcca ccacacgacg cttgccctcc
     7381 ggatcgatcc gaccgatcac cgtgaccatc cgtgccagct cggcgagctc gaagtcgcct
     7441 tccggcagcg ctcgcccgtc cttcggcgag atgcgcagcg gcagtccgac gtcggtcgcg
     7501 atcgagccga ccgtctcggg cgcatcgccg gagagcacct tgagctcgat accttcgtcg
     7561 agcaggtacg agactgtctc ccgtgcgttc gcgcggatgc cctccgcgag cacggcgata
     7621 ccgagcggtg tcagctcggg gggcgcgccg gtcgggtcgt ccggcagcgc ggcgatggtg
     7681 gtgccgatcg cgacgacgcg acggcccttg tcctgctcgg caaccacgag gtcggagagc
     7741 aatgcagggc tcacgacgtc cggcgccccg agcacgtagc gcgtgccggc cagctccaga
     7801 gcgctccacc gtcgcgccga cgagaacggc accgagccgg cgggcgggac ggcgacggcg
     7861 ccgagagcgg cgtcgatggc gtcgagggtg gcgttgcgat cttctgacgc cgccgcgtag
     7921 cgaccgaatg cgtctcgcac gctctgctcg tcgacgcccg gagccgggtg cagagcaacc
     7981 aggcgtggct cggactcagt cagcgttccc gtcttgtcga ggcagacgac gtcgacggag
     8041 gcgagcgact cgaccgcgtt cagctgctgc gccagtgcgc cctggcgcgc catccgcacg
     8101 gcggcggccg caaaggtgag cgaggccagc aggatcaggc cctcgggtac cagagtgacg
     8161 atgccggcca ccgcagtctc gacagcctgt cgggtctcga tctcgtgctg gatcagggct
     8221 gcgatgagga gcgcgccgag cggcaccatg atggcgacga gcaggtagag aagccggttg
     8281 agcgcctgct cgaggggcga tctcgggtga cggaattcct tggcgacgcc cgctacccgt
     8341 tcggcgtagc tgtcaggtcc gaccgcagtc acctcgtagc tgccggcgcc ctcgacgacg
     8401 aaggagcccg acagcactcc ttcgccgacg tcacgccgta ccgggtcgga ctcg
//
LOCUS       DBNGNK010000383         3136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1498000,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000383 DBNGNK010000000
VERSION     DBNGNK010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1498000"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="AC150V_11365"
     CDS             complement(<1..1281)
                     /locus_tag="AC150V_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763559.1"
                     /translation="MLLRFLLLLCGVVLVGAAARGGAAESDCVPRASAAYSKRVAAAL
                     GSGRDLWGEALLRAGSGPSFAGAAGRLAPILYARGPQDRLPTASGVYYLAFSMPRGSA
                     GAAEVMLHVADGSEILARRTTGASVEVSVGAMGREVYGSCPTRLAAAQLAEGGLPILR
                     TAYRDESGVRYTQESFAARAEGKLASFLRLTVDSDTAVVLRVGSLSLDVSPETAQTVY
                     VSWVGSGRPARIDADAYGAARASVDAYWRGRLAQGARIEVPEERLQDAQRALLSRALS
                     LTWRYSIGNAYEQFSFPETIDSARVVGEYGFAAVERAILLAALPARPTPYPNWKKGEK
                     LLGFALHYRRYRDRATLTRVTPTLRRFVTELERQLRPNGLLPRERYSSDIPDQVYGLH
                     AQARVWQGLREIAVAWSDAGRPELSARARKLAVRL"
     gene            1305..2516
                     /locus_tag="AC150V_11370"
     CDS             1305..2516
                     /locus_tag="AC150V_11370"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR3763560.1"
                     /translation="MKTVLIVGAGRYQRAVIRRAGELGLRVVAVDRNPDAPGLPEADL
                     ARVVDFSDPEAVLAGISDLEIDGVTTVQAERAVPMVATIAEALDLPGIGAETAHLMTH
                     KAAMREALARAGVPQPRFTTLRKAADARRAAEQVGFPAVLKPGAGSGQHGVFRVSSAS
                     QVEAHLDETIAVSPGQDAVLEEYVDGTEMNGIVIVRDGEVLSVILCDRLRPPGPSFGV
                     SWIHLYPPVVEGEQLVESERVAVDAVRALGLSSGIAFPQLIATPDGRIVVVEVAARIG
                     GLMAEMIRHALGIDILDVQLRIALGEEVPDELVRNRFQQPVAVRFLTAEPGPLPTGRV
                     TRVGPLDEVLAQPGVVEAALYVEEGEVIRPVMVISDRRGYIIAIGDTSGEALERAEAA
                     ARLVEVEVEAG"
     gene            2516..>3136
                     /locus_tag="AC150V_11375"
     CDS             2516..>3136
                     /locus_tag="AC150V_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763561.1"
                     /translation="MGTGRDSRCDFSLEHYRELLEAANAGGYQFAGFDRQPEPGLLFI
                     RHDVDLSLEAAVRMAEVEADVGAWSTWCLMTRSVFYNLDSEQGEWAIGRLRELGGRIA
                     HHAVHPHVDLDERFEPVVAWHNPDLDYLTDPINGATNPMSPPWYDPPHYRSDSNQHWH
                     YRGREGLCPHGELERGELDWLHLLIHPAIWAYDGATMKDTMESFVAA"
BASE COUNT          495 a         1106 c         1096 g          439 t
ORIGIN      
        1 gagacgcacg gccagcttcc gggcgcgggc ggaaagctcg gggcgccctg catccgacca
       61 ggcgacggcg atctcgcgca gcccctgcca cacgcgcgcc tgcgcgtgca gtccgtaaac
      121 ctgatcggga atgtccgagg agtagcgctc gcgtggcagc aaaccgttcg ggcggagttg
      181 ccgctcgagc tcggtgacga agcggcggag cgtaggcgtt acgcgcgtga gcgtcgcgcg
      241 gtctcggtac cgccgatagt ggagtgcgaa gccgagcagc ttctcgccct tcttccagtt
      301 cggatacggc gtcgggcgcg caggcagggc ggcgagcagg atggcccgct cgacggccgc
      361 gaagccgtac tcgcccacga cgcgcgcgct gtcgatcgtc tcgggaaacg aaaactgctc
      421 gtaggcattg ccgatgctgt agcgccaggt gagtgagagg gcacggctga gtagcgcacg
      481 ctgggcgtcc tgcagccgct cttcgggcac ctcgatccgc gccccttgcg cgaggcgccc
      541 acgccagtac gcgtcgaccg aggcccgcgc cgcgccgtag gcgtcggcgt cgatccgcgc
      601 cggtcgtccc gaccccaccc aagagacata gacggtctgt gccgtctccg gtgacacgtc
      661 gagcgagaga gaacccacgc ggagcaccac cgccgtgtcc gaatccacgg tgagacgcag
      721 gaagctcgcg agctttccct ccgcacgcgc cgcgaacgac tcctgcgtgt agcgaacacc
      781 ggactcgtcc cgataggcgg tgcggagtat cggcaacccg ccctcggcga gctgtgccgc
      841 cgcaaggcgg gtcgggcacg acccgtagac ctcgcgcccc atcgcaccca cggagacctc
      901 gaccgaggcg cccgtcgtac ggcgggccag aatctcgctt ccgtcggcaa cgtggagcat
      961 cacttcggcc gctccggccg agccacgcgg catcgagaag gccaggtagt agacaccgga
     1021 ggccgtcggc agccgatcct gcgggcctcg cgcgtagaga atgggcgcca gccgccccgc
     1081 agcgcctgca aacgacgggc cgcttccggc gcggaggagc gcctcgcccc agagatcgcg
     1141 cccggagccg agcgccgccg ccacgcgttt cgagtaggcg gccgaggcgc gcgggacgca
     1201 gtcggattct gctgcaccac cgcgcgccgc tgcgccgacg agaacgacgc cacacaggag
     1261 caggaggaac cgcagaagca tgctctcagt agcatcgcag cacgatgaag acagtcctga
     1321 tcgtcggagc cggccggtac cagcgcgccg tcatcaggcg ggccggtgag ctcgggctgc
     1381 gagtcgtcgc cgtcgaccgc aatccggatg caccggggct gcccgaggcc gacctcgcgc
     1441 gagtggtcga cttctccgat cccgaggccg tcctggccgg gatcagcgac ctggagatcg
     1501 acggcgtgac gacagttcag gccgagcgcg ccgttccgat ggtggcgacg atcgcggagg
     1561 ccctcgacct gccgggcatc ggcgccgaga ccgcgcacct gatgacccac aaggcggcga
     1621 tgcgcgaagc actcgcccgg gccggggtgc cgcagccgcg gttcacgacc ctgcggaagg
     1681 cggccgacgc tcgccgtgcg gcggagcagg tcgggttccc cgccgtcctg aagccaggcg
     1741 ccggcagcgg gcagcacggc gtcttccgcg tgagctccgc ctcgcaggtc gaggcgcatc
     1801 tcgacgagac aatcgctgtc tcgcccggcc aggacgcagt cctcgaggag tacgtcgacg
     1861 gcaccgagat gaacgggatc gtgatcgtgc gcgacggaga ggtgctttcg gtgatcctct
     1921 gcgaccgcct ccgccctccc gggccgagct tcggcgtgag ctggatccac ctctacccac
     1981 ctgtcgtcga gggcgagcag ctggtcgagt cggagcgggt cgcggtcgat gccgtacgag
     2041 cgctcgggct ctccagcggc atcgccttcc cgcagctgat cgcgacgccc gacgggcgga
     2101 tcgtcgtggt cgaggtcgcc gcccgcatcg gcggcctgat ggcggagatg atccgccatg
     2161 cgctcggcat cgacatcctc gacgtccagc tccgaatcgc gctcggtgag gaggttccgg
     2221 acgagctcgt gcggaaccgc tttcagcagc ccgtcgcagt ccgctttctg accgccgaac
     2281 cggggccgct cccgaccggc cgcgtcactc gcgtcgggcc gctcgacgag gtgctcgccc
     2341 agccaggtgt ggtcgaggcg gcgctgtacg tcgaggaggg cgaggtgatc cgcccggtga
     2401 tggtgatctc cgaccgccgc ggctacatca tcgccatcgg cgacacgagt ggcgaggcgc
     2461 tcgagcgcgc cgaggccgct gcccggctgg tcgaggtcga ggtcgaggct ggctgatggg
     2521 aaccgggcgc gacagtcgct gcgacttctc gctcgagcac taccgcgagc tgctcgaggc
     2581 cgcgaacgcg ggcggctatc agttcgcggg cttcgaccgg cagccggagc cgggtctgct
     2641 cttcatccgc cacgacgtcg acctctcgct ggaggcggcg gtgcgcatgg cagaggtcga
     2701 ggcagacgtc ggcgcttggt cgacgtggtg cctgatgacc cgatcggtct tctacaacct
     2761 cgactcggag cagggcgagt gggcgatcgg tcgcctgagg gagctgggcg gtcgcatcgc
     2821 ccatcacgcc gtccaccccc acgtcgatct cgacgagcgc ttcgagcccg tggtcgcctg
     2881 gcacaacccc gacctcgact acctgaccga cccgatcaac ggtgcaacga acccgatgag
     2941 cccgccctgg tacgacccgc cgcattaccg atcggactcg aaccagcatt ggcactaccg
     3001 cggccgcgaa ggcctctgtc cgcacggcga gctcgaacgc ggcgagctcg actggctcca
     3061 tctgctgatc catcccgcca tctgggcata cgacggcgcc acgatgaagg acacgatgga
     3121 gtcgtttgtc gccgcc
//
LOCUS       DBNGNK010000384         2439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1141125,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000384 DBNGNK010000000
VERSION     DBNGNK010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1141125"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC150V_11380"
     CDS             <1..195
                     /locus_tag="AC150V_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR3763562.1"
                     /translation="VTVTGTVSGAVDDPFGNTKLAFDLTAVVNRHDFGLSWNAPLPGG
                     GSILGDEVTLRGKLFFVKEA"
     gene            198..737
                     /locus_tag="AC150V_11385"
     CDS             198..737
                     /locus_tag="AC150V_11385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017886493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR3763563.1"
                     /translation="MRILALSGSLRRDSYNTDLLRLAAAEAPPGVAIDVFEGLREVPP
                     FDADEETTPGPAVVSLREAIGSADALLVATPEYNGSAPGVLKNALDWASRPAGGSALS
                     GIPTAVVGASPGRFGAAWAQADLRKVLGIAGSRVVDVELAVAKAHERLREPDDDLRAD
                     VKRVVDTLVQEGRALAEAA"
     gene            complement(742..1929)
                     /locus_tag="AC150V_11390"
     CDS             complement(742..1929)
                     /locus_tag="AC150V_11390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763564.1"
                     /translation="MRRPFVLALLVLGLVVLLAGCGGGGAGDGGSDQSGGSLAPAAGT
                     DFHPVAGDFEPNDQKLATCDPDQADYYSCLEQGFGNLAFEKGGKAAMKRFAADIAASE
                     DVERGCHRIAHTIGSATLARHEDDVGQAFADGDSTCWSGFYHGILERALKGATEESVL
                     VARLRELCAGVLEGPDRFIAYQCVHGLGHGLMIRSGLDLPGSLEMCTRLETEWEQISC
                     DGGVFMENFNTSYGIKSRFLKEDDPIYPCTAVKERHKLYCYLQITDNILALNGWDFPA
                     AALTCLDAEPNWVATCFQSYGRSSSGTARNDLELLNRYCGMVVPQWRGECIYGAVRDI
                     VSNDAHGDRALVYCNSVNALYAPRCFYGLGTIIHDLDGLDWCNRAPAEYRPNCRLRDT
                     GLG"
     gene            1971..2420
                     /locus_tag="AC150V_11395"
     CDS             1971..2420
                     /locus_tag="AC150V_11395"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR3763565.1"
                     /translation="MKVTMLLADYAVVADDKLTVVGGGWSIIGPEPTPFGIGILVHVP
                     WDQANTRHTMRLELLDSDGDPVLGRDEGDESTEPEPIVFLDEMSFEVGRPPGIKPGTP
                     LEVMIAVNSAPLTLEPGGRYEWRLSIDGESRDDWRVAFSVREDDAQL"
BASE COUNT          404 a          807 c          835 g          393 t
ORIGIN      
        1 gtcacggtga cgggaaccgt ctcgggggcc gtcgacgatc cgttcggcaa cacgaagctc
       61 gcgttcgacc tgaccgccgt cgtcaaccgt cacgacttcg gcctttcctg gaacgcgcct
      121 cttcccggcg gaggctcgat cctcggagac gaggtcacgc tccgcggcaa gctcttcttc
      181 gtgaaggagg cttgacgatg cgtatcctcg ctctctccgg aagcctccgg cgcgactcgt
      241 acaacaccga tctgcttcgc ctggcggcgg ccgaggcgcc gccgggagtg gcgatcgatg
      301 tgttcgaagg tctccgcgag gtgccgccgt tcgacgccga tgaggagacc accccggggc
      361 cggccgtcgt ctcgctgcgc gaggcgatcg gctcggctga cgcgctgctc gtagcgacac
      421 ccgaatacaa cggctccgct ccgggcgtgc tgaagaacgc actcgactgg gcctcgcgcc
      481 cggccggagg cagtgcgttg agcggaatcc cgacggcggt cgtcggcgcg agccccggcc
      541 gcttcggcgc cgcctgggcg caagccgatc tccgcaaggt gctcgggatc gcggggagtc
      601 gcgtcgtcga cgtcgagctc gcggttgcga aggcccacga acgactgcgc gagccggacg
      661 acgacctgcg cgcggacgtg aagcgcgtcg tcgacacgct cgtgcaggag ggcagggctc
      721 tggccgaagc cgcctgagcg gctagccgag ccctgtgtcg cgcagacggc agttcggccg
      781 gtactccgcc ggggcacgat tacaccagtc gagcccgtcc aggtcgtgga tgatcgtgcc
      841 gagcccgtag aagcaccggg gtgcatagag ggcgttgacc gagttgcagt acacgagtgc
      901 tctatcgccg tgtgcgtcgt tggagacgat gtctcgaacc gcaccgtaaa tgcactcgcc
      961 gcgccactgg ggaacgacca tgccgcaata ccggttcagc aactccaggt cgttccgcgc
     1021 agtgcccgag gagcttcgcc cataggactg gaagcaagtc gcaacccagt tcggctctgc
     1081 gtcgaggcac gtcagcgcgg ccgccggaaa atcccagccg ttcagggcga ggatgttgtc
     1141 ggtgatctgc agatagcagt agagcttgtg gcgctccttc acggcggtac agggatagat
     1201 cgggtcgtcc tccttcagga aacgcgactt gatgccgtac gaggtgttga agttctccat
     1261 gaagacgccg ccgtcgcagg aaatctgttc ccactcggtc tcgagccgtg tgcacatctc
     1321 gagcgatccg gggaggtcga gcccgcttcg gatcatcagg ccatggccga gtccgtgcac
     1381 gcactgatag gcgatgaagc gatcgggccc ctcgagcacg cctgcacaca gctcacgcag
     1441 gcgggcgacg aggacgctct cctccgttgc ccccttcaac gctcgctcga ggatgccgtg
     1501 gtagaagccc gaccagcacg tcgagtcgcc gtcggcgaac gcctggccga cgtcgtcctc
     1561 gtgccgcgcg agagtggccg agccgatcgt gtgtgcgatc cggtgacaac cgcgctcgac
     1621 gtcctcgctc gcggcgatgt ccgcggcgaa gcgcttcatc gccgccttgc cgcccttctc
     1681 gaaggcaagg tttccgaacc cctgctcgag gcagctgtag tagtcggcct ggtcgggatc
     1741 acacgtcgcg agcttctggt cgttcggctc gaaatcgcct gccactggat ggaagtcggt
     1801 acccgcggcc ggggcgaggg agccgccgct ctggtcggag ccaccgtcgc cggcgccccc
     1861 accgccgcaa ccggcgagga ggacgacgag cccaagaacg agcagggcga gaacgaacgg
     1921 ccttctcata ccccacaggg taggtacggt tccgccccga ggctagtggg atgaaggtga
     1981 cgatgctgct tgcggactac gccgtcgtgg ccgacgacaa gctgaccgtc gtcggcggcg
     2041 ggtggtcgat catcggcccc gagccgacac cgttcgggat cggcatcctc gtacacgtcc
     2101 cgtgggacca ggcgaacacg cgtcatacga tgcgcctcga acttctcgac tccgacggag
     2161 acccggtgct tgggagggac gagggcgacg agagtacgga gcccgagccc atcgtcttcc
     2221 tcgacgagat gtccttcgag gtcgggcgtc cgcccgggat caagccgggc acgccgctgg
     2281 aggtcatgat cgcggtcaat tcggcaccgc tgacgctgga gccgggcggg cgctacgagt
     2341 ggcgcctctc gatcgacggc gagtcacggg acgactggcg agtcgcgttc tccgttcgcg
     2401 aggacgacgc ccagctgtag acgggcgcgt cacgagccg
//
LOCUS       DBNGNK010000385         2260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1929470,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000385 DBNGNK010000000
VERSION     DBNGNK010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1929470"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="AC150V_11400"
     CDS             complement(<1..485)
                     /locus_tag="AC150V_11400"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR3763566.1"
                     /translation="MNSALYVGTVMHARRSPSENVFRYPVYMTLLDLDELAELDRNLP
                     LFGWNRSAVTAYRDTDHVDVRSLLDRNGIDVGPGGRIEVLTNLSVLGYVFNPVSFWWC
                     RRADGELAAVVAEVSNTFGETHPYVLLPGGGDAPARRGWQTDKRLHVSPVMSMEPDDT
                     GG"
     gene            complement(482..1675)
                     /locus_tag="AC150V_11405"
     CDS             complement(482..1675)
                     /locus_tag="AC150V_11405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3763567.1"
                     /translation="MTIAIVGTGIAGLGAAYALSRAQDVELFEARADAGGHTRTLRHS
                     GLALDTGFIVHNEINYPGLTRLLHELEIPTQQSEMSFSVECSCGVAWSSRRPWRAGPR
                     LLVEILRFLHSAERADASGRTIDGFLDDERYSESFRWHYLVPMTAALWSAAPGSALQL
                     PAPFVISFFRNHGMLGLRRHHWRTVVGGSATYVRALLERIGGRLHTRLPVRSVERVGG
                     GIELRAGDEPARRFDAVVFAVPAPEALGLLADASDDEQRLLGAFQTTTNETVLHTDSR
                     LLPRRKGDRASWNYQSPACRTPAPQPTVTYSLNRLQRLDAGEEYCVTLNRTAEIATES
                     VLATYQDSHPQMTLESEAARTELHRLNGLRNTAFAGAWQGYGFHEDGLESGLRAAASL
                     GARRP"
     gene            1723..>2260
                     /locus_tag="AC150V_11410"
     CDS             1723..>2260
                     /locus_tag="AC150V_11410"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR3763568.1"
                     /translation="MTDVTVGTVRGRTSRASRAVTLAAVVIPPLGLLSAAGLLWGVAL
                     SWVDVVLFVVFYVFCGLGITVGYHRLFSHKSFETSPWLRGSLAILGAMTTQGPVTQWV
                     TDHRKHHALSDRAGDPHSPHASHGDGTRGRVRGFVHAHVGWLFTTKGMERGEHYGRDL
                     YADPVVRVIDRLYFAWVAL"
BASE COUNT          347 a          741 c          796 g          376 t
ORIGIN      
        1 ccccccgtgt cgtccggctc catcgacatg acgggagaga cgtgaaggcg cttgtccgtc
       61 tgccatccgc gccgggccgg cgcgtcgccg ccgccgggaa gcaggacgta cggatgcgtc
      121 tctccaaagg tgttgctgac ctcggcgacg actgcggcga gctcgccgtc ggcgcgccgg
      181 caccaccaga aggagaccgg gttgaagacg tagccgagaa cgctcaggtt ggtgagcacc
      241 tcgatccggc cgccggggcc gacgtcgatg ccgttgcggt cgaggagcga ccggacgtcg
      301 acgtgatctg tgtcgcggta ggctgtcacg gcacttcggt tccagccgaa gagcggcagg
      361 ttgcggtcga gctccgcgag ctcgtcgagg tcgagcagcg tcatgtagac cgggtagcgg
      421 aacacgttct cgctcggaga ccgacgcgcg tgcatgacag tcccgacgta gagggcggag
      481 ttcacggtcg tcgtgctccc agcgaagccg cggcccgcaa ccccgactcg aggccgtcct
      541 cgtggaagcc gtacccctgc caggctcctg cgaaggcggt gtttctcagc ccgttgagcc
      601 ggtggagctc cgtccgagcc gcctcgctct cgagtgtcat ctgcggatga gagtcttggt
      661 aggtcgcgag cacggactcc gtcgcgatct cggcagtgcg attcagggtg acgcagtatt
      721 cctcgcccgc gtcgagtcgt tgcagtcggt tgagcgaata ggtgaccgtg ggctgcggcg
      781 ccggcgttcg acaggccggc gattggtagt tccacgaggc gcgatcgccc ttccggcggg
      841 gaagaaggcg gctgtcggtg tggagcacgg tctcgttggt cgtcgtctgg aaggctccca
      901 gcagccgctg ttcgtcgtcg gacgcgtcgg ccagcaaccc gagcgcctcg ggtgctggca
      961 cggcgaagac gaccgcgtcg aatctgcggg ccggctcgtc gccggcgcgc aactcgattc
     1021 cgccgcccac ccgctccacc gagcggacgg gtagccgggt gtgcaggcgt cctccgatcc
     1081 gctcgagcag cgctcggacg taggtggcgc tgcctccgac cacagtccgc cagtggtggc
     1141 ggcggagccc gagcatgccg tggttgcgaa agaacgagat cacgaaaggc gccggcagct
     1201 gtagcgcgga accgggcgct gccgaccaga gcgcggccgt catcggaacg aggtagtgcc
     1261 aacggaagga ctccgagtag cgctcgtcgt cgaggaagcc gtcgatcgtc cttccggaag
     1321 cgtccgcccg ctcggcgctg tgcaggaagc gcaggatctc cacgagcaag cgcgggcccg
     1381 cccgccacgg tcgtctgctc gaccaggcga cgccgcacga gcactcgacg gagaacgaca
     1441 tctcggactg ctgcgtcggg atctcgagct cgtgcaagag acgggtcagg cccgggtagt
     1501 tgatctcgtt gtgaacgatg aagccggtgt cgagggccag gccggaatgc cgcagagtgc
     1561 gcgtgtgtcc gcccgcgtcc gctctcgctt cgaacaattc gacgtcctgt gcgcgagaga
     1621 gggcgtaagc ggcgcccagt ccggcgatcc ctgtgcccac gatcgctatg gtcacgtgac
     1681 cgtgctccaa gggccgtcgc cgacagagat ctccgtcaga acgtgacaga cgtcaccgtc
     1741 ggaaccgtgc gcggccgcac gagccgggcc agcagagcgg tcacgctcgc cgcggtcgtc
     1801 attcctccgc tcgggctgct ctccgccgcg ggcctgctct ggggcgtcgc cctttcgtgg
     1861 gtcgacgtcg tcctgttcgt cgtgttctac gtgttctgcg ggttggggat caccgttggc
     1921 taccaccgtc tcttcagcca caagagcttc gagacgagcc catggctccg tgggtcgctc
     1981 gcaatcctcg gcgcgatgac gactcaaggg cccgtgacgc agtgggtgac cgaccatcgg
     2041 aagcaccatg cgctctccga ccgcgcgggc gatccccatt ctccccacgc ctcgcacggc
     2101 gacgggacac gggggcgcgt cagggggttc gtccatgcgc acgtcggctg gctgttcacg
     2161 acgaagggaa tggagcgcgg cgagcactac ggtcgggatc tgtacgcgga tcctgtcgtc
     2221 cgggtgatcg accgtctcta cttcgcttgg gtggcgctca
//
LOCUS       DBNGNK010000386         3801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1711620,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000386 DBNGNK010000000
VERSION     DBNGNK010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1711620"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="AC150V_11415"
     CDS             <1..307
                     /locus_tag="AC150V_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003082803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HIR3763569.1"
                     /translation="GWETIVAAYDRLLELQPSPVVRLNRAVAVALAGRVEEGLDEIDA
                     IHELDGYHLFHSARADLLRRLERREEAGREYHRAFELAGNEAERRFFERRLRELGEH"
     gene            complement(284..727)
                     /locus_tag="AC150V_11420"
     CDS             complement(284..727)
                     /locus_tag="AC150V_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006922234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG cluster 4 domain-containing protein"
                     /protein_id="HIR3763570.1"
                     /translation="MRTRQVSVIGSGSRHEPAAEAVGRLLAERGCTVVTGGLDEVMAA
                     AMRGAKEAGGTTLAILPGESADAANRWADHVVVTGIGHARNLAVAASGDAVVAVGGRW
                     GTMTEIGFAMRLGRPVVTLGRPVVEGVPHAASPEEAVELVLAQLA"
     gene            747..2009
                     /locus_tag="AC150V_11425"
     CDS             747..2009
                     /locus_tag="AC150V_11425"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR3763571.1"
                     /translation="MSVRREARGGTLIAGGGYAGGTLAWRLGRRGCTIVSPESSMLFA
                     PLLPEAASGTLEPRHVVVPLRQLCRDAEFVLGRVESVDLEGHSVVVATDTGPRTIGWR
                     RLVLAVGSISRHAAVPGLAEHSMSFKDLPDAIALRNHVLRQLELASAAGDAHTACRHL
                     TFVFIGAGYAGVEGLAELSDLVDDAAPYYPHLDGLPRRWVLVDHAPKILHEIPTGLGD
                     YAARELRRRGVEIRVGTALASVDADGVVLSDATRIDTATVVWTAGVQANPVLTSWRLP
                     LDELGRVKVDAFLRVEGREDVWALGDCAAVPNRMTPGEVDPPTSQHALRQARALTRNL
                     TGEERPYGYRMLGQVATLGRYKGIADVLGLRFRRFPGWFITRTYHLYQLPLLSRKARV
                     VTDWTTSLLFRRDIAELSMLTRSRDRER"
     gene            2006..2659
                     /locus_tag="AC150V_11430"
     CDS             2006..2659
                     /locus_tag="AC150V_11430"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3763572.1"
                     /translation="MSGCDISALYGLRLTTPRLELRLGSGDELVDLGRLAEKGVHPPE
                     EMPFAVAWTDGIGTPGFLDSFLEHHRGRLDDWSPENWTLNLLVWEGSALVGTQALIGG
                     RFARERTVDTGSWLGRRFQRRGLGTEMRAAVLEFAFRELGAVRANSGWLEGNDASRGV
                     SARLGYREVGTVTQSPRGEPVVEHTVRLERAEWRCPISVGVSGFPPCRHLFGLSESP"
     gene            complement(2643..>3801)
                     /gene="ligD"
                     /locus_tag="AC150V_11435"
     CDS             complement(2643..>3801)
                     /gene="ligD"
                     /locus_tag="AC150V_11435"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR3763573.1"
                     /translation="GFSDAEIERVAGMLRPLERDDAPFADAPKMPRKGGEVVWLEPVL
                     VADVEFAEWTRKGRLRAPVYQGLRDDKPATTVRREREPVPQEIRRGRKTLRVSNQDKL
                     FWPDEGITKADLLAYYRDVAPVLLPHLRGRPFTMKRYPDGWQGKHFFQKDAPSHTPEW
                     VKTVPFSATTRDTRERRIVDYVLVDDELTLLWVVSMGCIDLNTWYSRADRPDRPDWVL
                     FDLDPSEGVGFAETIEVAQLVKEALDLLELESFAKTSGSRGIHVLVPIARRHTYEDSR
                     EFSSIVAGAIARAHPGLATTEWVKSKRRGVLIDANQNGAGKTIASVYSVRPRAGAPVS
                     TPLVWDEVRPGLEPTAFTMETVLDRVAQHGDLFAGVLSVKQSLGKALRAIR"
BASE COUNT          530 a         1384 c         1283 g          604 t
ORIGIN      
        1 cggctgggag acgatcgtgg ccgcgtacga tcgtctgctc gagctccagc cctcgccggt
       61 cgttcgcctg aaccgagcgg tcgccgtcgc actggccggg cgcgtcgagg aagggctcga
      121 tgagatcgac gccatccacg agctcgacgg gtatcacctg tttcactcgg cgcgagccga
      181 tctcttgcgc cgcctggagc gccgagagga agccggtcgc gagtatcacc gcgccttcga
      241 gctcgcggga aacgaggcgg agcgtcgctt cttcgaacgc cgcctacgcg agctgggcga
      301 gcactagctc gacagcctcc tcggggctcg ccgcgtgcgg taccccctcc acaaccggcc
      361 gcccgagggt cacgaccggg cgcccgagcc gcatcgcgaa gccgatctcc gtcatcgtcc
      421 cccaacgtcc cccgaccgcg acgacggcgt cgcccgatgc tgcgacggcc aggttccggg
      481 cgtggccgat gcccgtgacc acgacgtggt ccgcccagcg gttcgcggcg tctgcgctct
      541 ccccgggcag gatcgcgagg gtcgtcccgc cggcctcctt cgcccctcgc atcgcagccg
      601 ccatcacctc gtcgaggccg cctgtcacga cggtgcagcc gcgctcggcg aggagtcggc
      661 cgactgcttc ggccgcgggc tcgtgcctgc tgccgctgcc gatcaccgag acttgccgcg
      721 tccgcacacg tgcgatgcta ggcgccatgt cggtacggcg cgaggctcgc gggggaacgc
      781 tgatcgccgg gggcggctac gccggcggca cgctggcatg gcggctcggt cgtcgcggct
      841 gcacgatcgt cagcccagag agctccatgc tcttcgcgcc gcttctgccc gaggcggcgt
      901 cgggaacgct cgagccccgc cacgtcgtgg tgccactacg gcagctgtgt cgggacgccg
      961 agttcgtcct cggccgggtg gagagcgtcg acctcgaggg acactccgtc gtggtcgcca
     1021 cggacaccgg gccacgaacg atcggctggc ggcgactcgt cctcgcggtg ggatcgatct
     1081 cccgccacgc ggccgtgccc ggactcgccg agcattccat gagcttcaag gatctccccg
     1141 acgcgatcgc gctccgcaac cacgtcctcc gccagctgga gctggccagc gccgccggcg
     1201 acgcgcacac ggcgtgtcgg cacctcacct tcgtcttcat cggggcgggc tacgccggcg
     1261 tcgaggggct ggcggagctc tctgacctcg ttgacgatgc cgcgccctac tatccgcacc
     1321 tcgacggctt gcctcgacgg tgggtgctcg tcgaccacgc gccgaagatc ctccacgaga
     1381 tccctacggg actcggcgac tacgccgcgc gcgagctccg ccgacgcggg gtcgagattc
     1441 gcgtcggcac tgcgctcgcg tccgtcgacg ccgacggagt ggtgctgtcg gacgcgacga
     1501 gaatcgacac cgcgacggtg gtgtggacgg ccggcgtgca ggcgaatccc gtgctgacct
     1561 cgtggcggct cccgctcgac gagctcggcc gcgtcaaggt cgatgcgttc ctccgggtcg
     1621 aggggcggga agacgtctgg gcgctcggcg actgtgcggc ggtgccgaac cggatgacgc
     1681 ccggtgaggt cgacccaccc acctcgcagc acgccctccg ccaggcccgg gctctgacga
     1741 gaaacctcac gggcgaagag cgcccgtacg gctaccgcat gctcggacag gtggctacgc
     1801 tcggccgcta caaggggatc gcagacgtgc tgggcctccg ttttcgccgc tttccaggct
     1861 ggttcatcac gcgcacctac cacctgtacc agcttcccct gctctcccgg aaggcccgtg
     1921 tcgtcaccga ctggacgacg agccttctct tccggcgcga catcgccgag ctgtcgatgc
     1981 tgacccgctc gcgcgaccga gagcggtgag cggctgcgac atatccgctc tctacgggct
     2041 gcgcctgacg acaccacgcc tggagctacg gctcggctcg ggcgacgagc tcgtggactt
     2101 ggggagactg gcggagaagg gtgttcatcc tccagaggag atgcccttcg cggtcgcctg
     2161 gacggacggg atcggcacac caggctttct ggactccttt ctcgagcacc atcgcggccg
     2221 cctcgacgac tggtcgcccg agaactggac gctcaacctc ctcgtctggg agggctccgc
     2281 gctcgtcggc acccaggcat tgatcggcgg ccgcttcgcc cgcgagcgca ccgtcgacac
     2341 gggctcgtgg ctcggccgac gcttccagcg gcgcgggctg ggcacggaga tgcgcgcggc
     2401 ggtgctcgag ttcgccttcc gcgagctcgg ggcggtgcgc gcgaactccg ggtggctcga
     2461 ggggaacgac gcctcccgag gcgtgtcggc gagactcggc taccgggagg tcggcaccgt
     2521 gacacagagc ccccgcggcg agcccgtcgt cgagcacacg gtgcggctgg aacgcgcgga
     2581 gtggcgctgc cccatcagcg tcggcgtctc cggcttccca ccctgccggc acctgttcgg
     2641 gctcagcgaa tcgccctgag cgccttgccg agcgactgct tgacggagag cacgcccgca
     2701 aacaggtcgc cgtgctgggc aacccggtcg agaacggtct ccatcgtgaa cgccgtgggc
     2761 tccagtccgg gccgcacttc gtcccacacc aacggcgtcg agaccggtgc tccggcccgc
     2821 ggtcgcaccg agtagaccga ggcgatcgtc ttaccggctc cgttttggtt tgcatcgatg
     2881 agtacgccgc gtcgtttgct cttgacccac tccgtggttg ccagccccgg gtgggcccgc
     2941 gcgatcgcgc cggcgacgat cgacgagaac tcgcgggagt cctcgtacgt gtggcgtctc
     3001 gctatcggca cgagcacgtg gattccccgc gacccgctcg tcttcgcgaa gctctcgagc
     3061 tcgagcaggt cgagcgcctc cttcacgagc tgtgccacct cgatcgtctc cgcgaagccg
     3121 acaccctccg acgggtcgag gtcgaacagg acccagtcgg gccgatccgg gcggtcggca
     3181 cgcgagtacc aggtgttgag gtcgatgcag cccatgctca cgacccacag cagggtgagc
     3241 tcgtcgtcga ccagcacgta gtcgacgatc cgccgctccc tcgtgtcccg cgtcgttgcc
     3301 gagaagggca ccgtcttcac ccactccggc gtgtgggagg gcgcgtcctt ctggaagaag
     3361 tgcttgccct gccagccgtc ggggtagcgc ttcatcgtga agggccgacc gcgcaggtgc
     3421 ggcagtagca ccggtgccac atcccggtag taggcgagca ggtccgcctt cgtgatcccc
     3481 tcgtcgggcc agaacagctt gtcctggttc gagacgcgca gcgtcttgcg gccgcggcgg
     3541 atctcctgcg ggaccggctc tcgctctcgc cgcacggtcg tggccggctt gtcgtcccgt
     3601 aggccctgat agacgggggc ccggagccga cccttgcgcg tccactccgc gaactcgacg
     3661 tccgcgacga gcaccggctc gagccagacg acctcaccac ccttcctcgg catcttgggc
     3721 gcgtccgcaa acggagcgtc gtcccgctcg agcggacgca gcatacctgc gactcgttcg
     3781 atctccgcat cgctgaaccc g
//
LOCUS       DBNGNK010000387         3661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_352239,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000387 DBNGNK010000000
VERSION     DBNGNK010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_352239"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..456)
                     /locus_tag="AC150V_11440"
     CDS             complement(16..456)
                     /locus_tag="AC150V_11440"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR3763574.1"
                     /translation="MARAYTSTVIDAPAKDVWGHVRDFNGLPEWTGGLIAKSEIEEGK
                     EGDQVGAIRSFTLPDGTHFRERLLSHSDAQRAYSYNFEKTPFDVDSYHATLKVTPITD
                     GDRSFVEWWTTFDCNRDQIDHWEDFFANEIFQNALNAVKDHFAS"
     gene            complement(496..2166)
                     /locus_tag="AC150V_11445"
     CDS             complement(496..2166)
                     /locus_tag="AC150V_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3763575.1"
                     /translation="MSRSGGRILVDQLALHGADLAFGVPGESYIPVLDAVRDSPVRYV
                     TCRHEVGAANMADAYGKLTGRPGLCFVTRGPGATHASCGVHTAFQDSTPLILFIGQVA
                     RRSLDREAFQEIDYREMFAPMAKWVAQVMEAARIPELVSRAFHVATSGRPGPVVLALP
                     EDVLAERGDVSDSAPFESAQGRPGARELERMTEHLSGAARPLVVVGGHPWTRQAHADM
                     TAFCEASDLPVAASFRCQDYVDNASPSYAGHLTLGLDPKLATRVREADVLLVVGARLG
                     DVTTSGYTLLDVPRPRQTLIHVHAGAEELGRVYRPDVAIVSGPPQFAEAARALAPLDP
                     SAWRDETRQARADYLEYLRHQRHERALDMGDVMAYLRDRLPDDAVVTNGAGNFSVWAH
                     RFYEFRTFRTQLAPTSGAMGYGVPAAIAAKLVRPDRIVVAFAGDGDFLMTGQELATAV
                     QVGAAIVVLVINNGMYGTIRMHQERQFPGRVSATDLANPDFAAYAQSFGASGETVERT
                     EDFEPAFERALAVGGPALIELRVDPEAITPRQTIAEIRDAAREAQRTT"
     gene            complement(2163..2711)
                     /locus_tag="AC150V_11450"
     CDS             complement(2163..2711)
                     /locus_tag="AC150V_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3763576.1"
                     /translation="MRLSLYEFETGAETIGGHRVVYDVANDTASFGAAKVEGHALVWQ
                     LDESEDEAEGALLSHDVHLDPFAEWIVRCDRIDFPVGGIAYLHTHPGPGIRYLLHGEI
                     EINTEGRSTFYGPGGAWFEAGPDPVYAAASQHHDTAFVRVMVLPAQWEGKRTVSYVNP
                     EDADLPKLQRATVYFDHRIDLT"
     gene            complement(2942..>3661)
                     /locus_tag="AC150V_11455"
     CDS             complement(2942..>3661)
                     /locus_tag="AC150V_11455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3763577.1"
                     /translation="IVTGGAKGIGRAIADGLAAEGARIVIADLAGAEEAASSFADGVG
                     LSVDVADEEAVATMVQETTERCGSIDILVNNAGLYASLAMRPFTEIPLAEWRQVMDVN
                     VASMFLTCRAVVPVMRRQGGGNIVNISSGTPFRGVPFLLHYVTSKGAIVAFTRALAKE
                     LGKDGVRVNCVAPGFTMSDGVKEHPEVIEKLREVSVAARTIQRDQEPEDVVGAVVYFA
                     SPAAGFVTGQTMVIDGGQYFH"
BASE COUNT          558 a         1231 c         1271 g          601 t
ORIGIN      
        1 ccttgccggc ccgctctacg aggcgaagtg gtctttcacg gcgttcaggg cgttctggaa
       61 gatctcgttc gcgaagaagt cctcccagtg gtcgatctga tcacggttgc agtcgaatgt
      121 cgtccaccat tcgacgaagc ttcggtcgcc gtcggtgatc ggggtgacct tgagcgtcgc
      181 gtggtagctg tccacgtcga acggggtctt ctcgaagttg tacgagtagg cgcgctgcgc
      241 gtcggagtgg ctcaggagcc gctcgcggaa gtgcgtcccg tccggcagcg tgaagctgcg
      301 gatcgcgccc acctggtcgc cttcctttcc ctcctcgatc tcgctcttcg cgatcaggcc
      361 cccggtccat tccgggaggc cgttgaagtc gcgcacgtgt ccccatacgt ccttcgccgg
      421 tgcgtcgatc acggtgctgg tgtacgccct cgccatcgtg ctcctttccg aatgagtagc
      481 tccgctgcgg cgatcctacg tcgttcgctg cgcctcgcgg gccgcgtcgc ggatctcggc
      541 gatcgtctgt ctgggcgtga tcgcctctgg gtcgacacgt agctcgatca gggctgggcc
      601 gcccacagcg agtgccctct cgaatgcggg ctcgaagtcc tccgttcgct cgacggtctc
      661 gccggaggcg ccgaaagatt gggcataggc cgcgaagtcg ggattggcga gatccgtagc
      721 gctgacacgc ccgggaaact ggcgctcctg gtgcatgcgg atcgtgccgt acatgccgtt
      781 gttgatcacg agcacgacga tcgcggctcc gacctgcacc gccgtcgcca gctcctggcc
      841 ggtcatcagg aagtcgccgt cgccggcgaa ggcgacgacg atcctgtcgg ggcggacgag
      901 cttcgccgcg atcgccgccg ggacgccgta gcccatggcg ccgctcgtcg gggcgagctg
      961 cgtgcggaac gtgcggaact cgtagaagcg gtgggcccag accgagaagt tgccggcccc
     1021 gttggtgacg accgcgtcgt cgggaagcct gtcgcgcagg tacgccatca cgtcgcccat
     1081 gtcgagcgcc cgctcgtggc gttggtggcg caggtactcg aggtagtcgg cgcgcgcctg
     1141 ccgggtctcg tcccgccacg ccgacgggtc gagcggcgcg agggcacgcg ccgcctccgc
     1201 gaattgcggc ggcccgctca cgatcgcgac gtcgggccgg tacacgcggc cgagctcctc
     1261 tgcccccgca tggacgtgga tcagcgtctg tctcggacgc gggacgtcga ggagcgtgta
     1321 gccggacgtc gtgacgtctc ccaaccgcgc cccgacgacc agcaggacgt ctgcctcccg
     1381 cacacgggtt gccagcttcg ggtcgagccc gagggtgagg tgcccggcgt acgagggtga
     1441 cgcgttgtcg acgtagtcct ggcagcggaa ggaggccgcg acgggcagat cgctcgcctc
     1501 gcagaaggca gtcatgtctg cgtgcgcctg ccgcgtccag gggtggccac cgacgacgac
     1561 gagtggtcgc gccgcgccac tcagatgctc ggtcatgcgc tcgagctcgc gcgcgccagg
     1621 acggccctgt gccgactcga agggcgctga gtcggaaacg tctccccgtt cggccagcac
     1681 gtcttccggc agcgccagca cgaccgggcc cggtcgaccc gaggtcgcaa cgtggaacgc
     1741 acgggagacg agctccggaa tccgcgccgc ttccatcacc tgggcgaccc acttggccat
     1801 gggagcgaac atctcccggt agtcgatctc ctggaaggcc tctcggtcga gtgagcggcg
     1861 ggccacctgc ccgatgaaga ggatcagcgg cgtcgagtcc tggaaggcgg tgtgcacgcc
     1921 gcaggaggcg tgcgtcgcgc cggggccgcg ggtgacgaag cagagccccg gacggccggt
     1981 cagcttgccg tacgcgtccg ccatgttggc cgctcccacc tcgtgccggc aggtgacata
     2041 acggacaggc gagtcgcgca cggcgtcgag aaccgggatg tagctctcgc ccggaacgcc
     2101 gaatgccaag tcggcgccgt gcagcgcgag ctggtcgacg aggatccgcc cgcccgatcg
     2161 gctcacgtca gatcgatacg gtgatcgaag tagaccgtcg cccgctgcag cttcgggagg
     2221 tctgcatctt ctgggttgac gtagctgacc gtccgctttc cctcccactg ggcggggaga
     2281 accatcacgc ggacgaacgc cgtgtcatgg tgctgtgaag cggctgcgta gacggggtcg
     2341 ggacccgcct cgaaccaggc gcccccgggc ccgtagaacg tcgagcggcc ctcggtgttg
     2401 atctcgatct cgccgtgcag gaggtagcgg atgccggggc cgggatgagt gtgcaggtac
     2461 gcgatcccgc ccacgggaaa gtcgatccga tcgcagcgga cgatccactc ggcaaacggg
     2521 tcgagatgga cgtcgtgcga gaggagcgcc ccctccgcct cgtcctcgct ctcgtccagc
     2581 tgccacacga gcgcatggcc ctcgaccttt gcagcgccga aggatgccgt gtcgttggcg
     2641 acgtcgtaga cgacgcggtg gccgccgatc gtctcggcgc cggtctcgaa ctcgtacaga
     2701 gagaggcgca cggaacgagc gtatcgcggc attcgacgtg aggcggcggc cacccgcaac
     2761 ggcggcggcc accctcaacg agggccagat cggccacgtc ccgcctcgga ggaccggtgt
     2821 gacgagattg cgacgaaacg ctctcggctt gagcgcgggc gaagcccggc ctgcctcgcc
     2881 tcgctccgat gcacatgcga gaggaggagg ctcgtggggg aacaggggtt cccccacgtc
     2941 actagtggaa gtactggccg ccgtctatga ccatcgtctg tcccgtcacg aacccggccg
     3001 cgggcgacgc gaagtagacg acggcaccga ccacgtcttc cggctcctgg tcgcgctgaa
     3061 tcgtgcgggc ggcgaccgag acctcgcgca gcttctcgat tacctcgggg tgctccttga
     3121 cgccgtcgga catggtgaag ccgggggcga cgcagttgac gcgcacgccg tccttgccga
     3181 gctccttggc gagcgcgcgc gtaaacgcga cgatcgctcc cttgctggtg acgtagtgga
     3241 ggaggaaggg gacgccgcga aacggggtgc cggacgagat gttcacgatg ttgccgccgc
     3301 cctggcggcg catcacgggt acgaccgccc gacacgtgag gaacatgctg gccacgttca
     3361 cgtccatcac ctggcgccat tcggcaaggg gtatttcggt gaacgggcgc atcgccaatg
     3421 aggcgtagag cccggcgttg ttgacgagga tgtcgatcga gccgcatctc tcggtcgtct
     3481 cctgcaccat cgtcgccaca gcctcctcgt cggccacgtc gacggacagc ccgaccccgt
     3541 cggcaaaaga gctcgccgcc tcctcggctc ccgcgaggtc ggcgatcacg atcctggctc
     3601 cttccgccgc gagtccgtcg gcgatggcgc ggccgatgcc cttggcgcca ccggtgacga
     3661 t
//
LOCUS       DBNGNK010000388        10334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_211200,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000388 DBNGNK010000000
VERSION     DBNGNK010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_211200"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2179
                     /locus_tag="AC150V_11460"
     CDS             <1..2179
                     /locus_tag="AC150V_11460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3763578.1"
                     /translation="GPAGLTAAQDLAEEGYAVHVYELTAKLGGYMTHGIPAFRCPPEI
                     FQEDIDRMLHRCPGITVHLNTGLGEGVTLDELKVRHEAVLLTLGAWWGKGLRLDGPAS
                     AERVVDGVTFLRRINDGERPELPSSVVVVGAGDVAMDACRAALRLPGCKDVKVLYRRG
                     PDEIPARRDELEGALEEGVDLRYNVQPVAVLNGERPFSLRCIETELGAPGEDGRRLPV
                     DIAGSEHEVDCGLVILATGQKTVSDHLAERGLMDGEKIHTDWESMRTADPQVFAAGDG
                     AFGPSTIVTAMYHGHRAAYYVKAFLEGRESPLPYRTPFKTRRVPVAQDAQWEEFNREQ
                     QAFHGVGENPIAFPEIESTYDEEAARREAARCYRCDAETGSADYSVRSREDIFTMART
                     KPADGRKQRALFTRRLTLAGDRHFAPATATFDDLVFLPANLSRLVIDPYRDACNVRTK
                     LGEVLELGSPLLAAGFDGAPDEVRGAIAHGVAAQELAYIGRRPLGDVPWLQLVVAGED
                     QPDPAAAAVIGASADEQLASAPGPAREGQLVGLAARSNELRDVIPFALERGHDLLVLE
                     GNSRISGSWPELAGPPDLTAVRDAIKILRELDREEDIALLWFGGVRSGTDVAKLVALG
                     VSAATVGVSLGLAVGGQVQDGTLAFYGDIAAEERGEKADLFLNALRTEASIMPRCTGK
                     TDIHNLEPEDVRSISLTTAAAAGVPLAGENPRRAAESVTGDAD"
     gene            complement(2160..2963)
                     /locus_tag="AC150V_11465"
     CDS             complement(2160..2963)
                     /locus_tag="AC150V_11465"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR3763579.1"
                     /translation="MARTGQPSRGRVTSAGRSIAVLDALAEGGTLGTNEIARRTGSAP
                     STVSRQLGTLLEGGLVEFVEETGHYRLGLRLVQLANAVLARLDVRNVARPHLEALVDD
                     AGETATLSVPGDREAITVDFVASDRYVQGVTRLGRPSIGHATAAGKVMLADGVRSLQS
                     GELTAYTERTITDRTALAAELRRVRERGWAEAYEEREPGLNAIAAPIWSARGELEAIV
                     ALQGPIPRFGRRAARRALPALLRCAADVSAELGWIADRGRSRAVSPRRP"
     gene            3016..3270
                     /locus_tag="AC150V_11470"
     CDS             3016..3270
                     /locus_tag="AC150V_11470"
                     /inference="COORDINATES: protein motif:HMM:NF016179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HIR3763580.1"
                     /translation="MSMLVTCPNCGPREVEELRNAGEATRRPAVTPTARELNTYIYFR
                     ENAAGYLREWWFCRVCEDWFLAERNTVTNDVRTTWMPESA"
     gene            3267..5924
                     /locus_tag="AC150V_11475"
     CDS             3267..5924
                     /locus_tag="AC150V_11475"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR3763581.1"
                     /translation="MSRLGERPGERIDRGRSLSFTFDGKPYQGFEGDTIGSALYAAGR
                     RIFSRSFKYHRPRGLQCCVGHCANCQMTVDGIPNVRVCTTPLREGSVVAGQNTVGSLD
                     RDLMSVTDKLGGPFTPPGFYYKTFIRPRRLWPLYEKVLRNAAGLGPLDPQGERPDRVD
                     VEYRHADVVVIGGGQAGLEAAAEAADRGESVIVVDEGPEVGGELLCDPQGAETARALS
                     DRARAAGAELLSPATAIGIFEQNLVPVAHGNLLLKIRATQVVVAGGVVEQPLVFPGND
                     LAGVVLPSAVRRLVNYWSILPGERAVVVTADDRGLRAAHDLQSAGAEVLEVVDLRQQQ
                     VTNLEVQGRKGRVSQIHLRDTWVKCDLVVMSGSPQPNTKLLAQAGARVEYDESRGIFV
                     PSELPASVAAVGSVTGEVGEPAVPAPILGHLGDKCFVCFCEDQTTKDLKYAIAEGFSS
                     IELSKRYTTVTMGPCQGRLCAVNAIRVHAKQNGVDENTIGTTTARPPYTPVTLGLLAG
                     RPHEPAKRTSIHHRHKDLGAEMMWAGPWRRPFAYGDDPGDEAQNVHRSLGLIDVSTLG
                     KLLVRGPDAGAFLDRVYPNRFSDLKLGRIRYGVLTTDAGRIMDDGTVARLGEELYYVT
                     TTSSGASAVYQWLTWWNAIWEMDVDFVDITGAIAAVNVAGPNARTLMERVADIDMSNE
                     AFTYLDAKHCGVAGVPTLALRIGFVGELGYELHFPSPHGEYLWDTLLERGDDLGARPF
                     GLEPQRILRLEKGHVIVGQDTDSESNLLEAAMPWIFKADKPDFVGKWGTQQVQLRGLD
                     WMLVGFESPSGAVPLEGGQVLVDGQSAGRVTSARFSAELGKVIGLAVIPNELAVDGGA
                     FDVQVDGRHVPMRVHLGPFFDPEGERLKA"
     gene            5921..6529
                     /locus_tag="AC150V_11480"
     CDS             5921..6529
                     /locus_tag="AC150V_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763582.1"
                     /translation="MSELAFISPGRAVDYRDMHPVARSPLDRRQRDAGATFEERDGWL
                     VPVSMPHEQEYLGRVGIADLSYLTKLEVRPAGEALEGEDIVWYEISPRRALVFCKPSD
                     EAGVREQLGSRATVDVTAALGILMVAGPEAPTLIRRLTHLHHFPSGGDVAHITAHVLE
                     EGGIYWIVFPQEEGHYLWEVAVDRALPLGGGPVGVDALGATA"
     gene            6526..7740
                     /locus_tag="AC150V_11485"
     CDS             6526..7740
                     /locus_tag="AC150V_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR3763583.1"
                     /translation="MKDVFFRKEILRTPAELKRHYDVVIVGGGSHGLATAYYLATQHG
                     ITDVAILEKSYIGSGAAGRNTTIIRSNYRTPEGAAFYKESVALYERLSTELDFNLMFS
                     QHGHFTLAHSDRSLVVQQERAEVNKLLGIDSRVIDAAEVKELCPQISISRDVTWPILG
                     ALYHPPGGIIRHDAVVWGFAKQVGRAGIDIHQGVEVTGFDIRGGKVHGVRTTQGDISC
                     QVAVSATAGWSSIVADMAGIPLPITTSILQAFVTEPVKPFLDKIIVSSQMHIYVSQTD
                     RGEFLIGAEVEPYPTYSNKGTFPFVEYACRHALELFPQLHATRILRHWTGLCDMSPDY
                     SPIMGVTEIDGFIVDCGWGTYGFKAAPITGLTLAELIATGEPPELIRPFTLERFYTDT
                     LVSELAAAAVSH"
     gene            7767..9422
                     /locus_tag="AC150V_11490"
     CDS             7767..9422
                     /locus_tag="AC150V_11490"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR3763584.1"
                     /translation="MLSSLEIAQAASLRPITDVAADAGIEPDELDAYGRHKAKVDLSV
                     LDRLAGKPDAKLINVTAITPTPAGEGKTTTSVSLTQGLGLLGRRPVLCLREASLGPVF
                     GIKGGAAGGGYAQVGPMEDLNLHFTGDIHAITAANNLLSAMIDAHLLHGNELGIDPLS
                     ISWRRCLDMNDRSLRDVATGLGGRVNGYPRQTGFDITAASEVMGLVAVARDLADLRER
                     LGAITVGATFEGEPVTAEQIKAAGSMAVLLKDAIKPNLVQTLEGQPAFVHCGPFANIA
                     HGNNSLVADRVALKLGDYMITESGFASDMGMEKFFDITCRMGGLRPSAVVLVATVKAL
                     KHHGGDIDGGLAELETGAANLARHIGIIKGFGLEAVVGVNRFPTDSDEELETVRRLAL
                     EHGAHAAEINEAFQRGGEGARALAEAVVDAAEQPNEFDFAYPSDASIEEKIRAIATKV
                     YGADGVELLPAAKQAAERFEHAGLGSLPICMAKTHLSLSHDPALRNAPTGFTVPVRDL
                     RPYTGAGWIVALCGDMMTMPGLGKAPAAFAIDVDSTGDTVGLF"
     gene            9545..10006
                     /locus_tag="AC150V_11495"
     CDS             9545..10006
                     /locus_tag="AC150V_11495"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR3763585.1"
                     /translation="MRILVAYDGFEQSRPALEEAVRIALTVSPGIEGVRAPTEVIVLS
                     VVPPPEQMPNPGGVAGMRPHAVDDVAEAHRFLKERGIESEMKITHGKPADEILREATE
                     RKCDRIVMGTRGLGKMGRLLQGSVSTKVTKNAPCTVVIATAGKMERIEPSG"
     gene            complement(10051..10275)
                     /locus_tag="AC150V_11500"
     CDS             complement(10051..10275)
                     /locus_tag="AC150V_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763586.1"
                     /translation="MPVLLSCGLCQVKAVDGIVSRSSWGHIPTNPHGELHACPVCLEK
                     YPNEWQQRVLAANPAEASGSAPKKDPWGRS"
BASE COUNT         1686 a         3538 c         3530 g         1580 t
ORIGIN      
        1 cgggccggcg gggctgacgg cggcccagga tctcgccgag gagggctacg cggtgcacgt
       61 ctacgagctg accgcgaagc tcggcggcta catgacgcac ggcatcccgg cgttccgctg
      121 tccgcccgag atcttccagg aggacatcga ccggatgctg caccgatgtc cgggcatcac
      181 ggtgcacctg aacaccggtc tcggagaggg tgtcacgctg gacgagctga aggtgcgcca
      241 cgaggcggtg ctgctgacgc tcggtgcgtg gtggggcaag ggtctccgtc tcgacggacc
      301 cgcctccgcc gagcgggtgg tcgacggggt cacgtttctg cggcggatca acgacggcga
      361 gcggcccgag ctgccctcgt cggtcgtcgt cgtcggcgcc ggcgacgtcg cgatggacgc
      421 ctgccgagcc gcgctccgcc tgccgggctg caaggacgtc aaggtgctgt accgtcgcgg
      481 gcccgacgag atcccggcac ggcgggacga gctcgaaggc gcgctcgaag agggagtcga
      541 cctgcgctac aacgtgcaac ccgtcgcggt gctcaacggc gagcgtccct tctcgctgcg
      601 ctgcatcgag acggagctcg gcgcgccggg tgaagacgga cggcgtctcc ccgtcgacat
      661 cgcgggttcg gagcacgaag tcgactgcgg cctggtgatc ctcgcaacgg gccagaagac
      721 ggtctccgac catctggccg aacgcggctt gatggacggc gagaagatcc acaccgactg
      781 ggagtcgatg cgcactgccg acccgcaggt cttcgccgcg ggcgacggag ccttcgggcc
      841 gtcgacgatc gtcaccgcca tgtatcacgg tcatcgtgcc gcgtactacg tcaaggcgtt
      901 cctcgagggt cgcgagagcc cgcttcccta tcgcacaccc ttcaagacac gccgggtgcc
      961 cgtggcgcaa gatgcgcagt gggaggagtt caatcgcgag cagcaggcct tccacggagt
     1021 cggcgagaac ccaattgcct ttcctgagat cgagtccacc tacgatgagg aagcagcacg
     1081 ccgcgaggcc gctcgctgct accgctgcga cgcggagacc ggctcggccg attacagcgt
     1141 ccggtcgcgc gaggacatct tcacgatggc gcgcacgaag ccggccgacg ggcgaaagca
     1201 gcgggcgctg ttcacgcggc ggttgacgct cgccggcgac cggcacttcg ctccggcaac
     1261 cgcgactttc gacgatctcg tcttcctgcc ggcgaacctc tcccgtctgg tgatcgatcc
     1321 gtaccgtgac gcctgcaacg tccgcacgaa gctgggtgag gtgctcgagc tcggctcgcc
     1381 gcttctcgct gccggcttcg acggcgcgcc cgacgaggtg cggggcgcga tcgcgcacgg
     1441 cgtcgcggcg caggagcttg cgtacatcgg gcgccggccg ctcggagatg tgccctggct
     1501 gcagctcgtc gtcgcgggcg aggatcagcc cgatccggcc gcggcggccg tgatcggagc
     1561 atcggccgac gagcagctgg cgagtgcgcc cgggcccgcc cgcgagggcc agctcgtcgg
     1621 gctcgcggcg cggtcgaacg agcttcgcga cgtcattccg ttcgctctcg agcgcggcca
     1681 cgatctgctc gttctcgagg gaaactcacg catctccggg tcctggccgg aactcgccgg
     1741 accgcccgat ctgaccgccg tcagggatgc gatcaagatc ctgcgcgagc tcgatcggga
     1801 ggaagacatc gccctcctct ggttcggggg cgtccgctcg ggcaccgacg tggcgaagct
     1861 cgtcgcactc ggagtctcgg ccgccaccgt cggcgtctcg ctcggactcg ccgtcggcgg
     1921 acaagtgcag gacggaacgc tcgccttcta cggcgacatc gcagccgagg agcgaggcga
     1981 gaaagccgac ctcttcctga atgcgcttcg caccgaagcc tcgatcatgc cccgctgcac
     2041 gggcaagacc gatatccaca atctggagcc cgaagatgtc cgctcgatct cgctcacaac
     2101 ggccgcggcc gccggtgtgc ctctggcagg ggagaacccg aggcgtgccg ccgagtcggt
     2161 cacgggcgac gcggactgac agctcggctc cggcctcggt cggcaatcca gcccaactcg
     2221 gccgagacgt cggcagcgca gcggagcagt gcggggagcg cgcggcgcgc cgcgcgacgg
     2281 ccgaagcgcg ggatcggccc ttgcagcgcg acgatcgcct cgagctcgcc ccgcgccgac
     2341 cagatcggtg cggcgatcgc gttcagcccc ggctcgcgct cctcgtacgc ctccgcccaa
     2401 ccacgttcgc gcacccgccg gagctccgcc gcgagggcgg tacggtctgt gatcgtccgc
     2461 tcggtgtagg cggtgagctc cccggactgc agggaccgca cgccgtcggc gagcatcacc
     2521 tttccggccg ccgtcgcatg accgatcgag gggcgcccga gtcgcgtcac gccctgcaca
     2581 tagcggtcgc tcgccacgaa gtcgacggta atcgcctcac ggtcgccggg aacggagagc
     2641 gtcgcggtct cgcccgcgtc gtcgaccagc gcctcgaggt gcggccgcgc gacgttccgg
     2701 acgtcgaggc gcgcgagcac tgcattcgcc agctgaacca gccggaggcc caggcgatag
     2761 tggccggtct cctcgacgaa ttcgacgaga ccgccctcga gcagcgtgcc gagctggcgc
     2821 gagaccgtgc tcggagcgct cccggttcgc cgtgcgatct cattcgtgcc gagcgtgcct
     2881 ccctcggcga gcgcatcgag gactgcgatg gatcggccgg ccgacgtgac ccgccctcgt
     2941 gacggttgac ccgtgcgcgc cacgcacggt agtattgcgc atcccagcat tccattgcaa
     3001 ataacggaat aagcgatgtc aatgctcgtc acctgcccga actgcggtcc tcgcgaggtc
     3061 gaggagctcc gcaacgccgg cgaggcgacg cggcggccgg ctgtcacgcc gaccgcgcgc
     3121 gagctgaaca cctacatcta cttccgtgag aacgccgccg gctacctgcg cgaatggtgg
     3181 ttctgccgcg tatgcgagga ctggttcctg gcagagcgca acacggtcac gaacgacgtg
     3241 cgcacgacgt ggatgcccga gagcgcatga gccgtctggg cgaacgaccc ggcgagcgga
     3301 tcgaccgagg acggtctctc tccttcacct tcgacggtaa gccgtaccag ggattcgagg
     3361 gcgacaccat cggctcggcg ctgtacgcgg cgggacggcg catcttctca cgcagcttca
     3421 agtaccaccg cccgcgtgga ctgcagtgct gcgtcggtca ctgcgcgaac tgccagatga
     3481 ccgtcgacgg gatccccaac gtacgggtct gcaccacgcc gttgcgggaa ggctcggtcg
     3541 tcgccggcca gaacaccgtc ggctcgctcg accgcgacct gatgagcgtg accgacaagc
     3601 tgggcggccc cttcacgccc ccgggcttct actacaagac cttcatccgt ccacgcaggc
     3661 tctggccgct ctacgagaag gtgctgcgaa acgccgccgg actcggcccg ctggatccgc
     3721 agggagagcg gcccgaccgg gtagacgtcg agtaccgtca cgccgacgtc gtcgtgatcg
     3781 gcggcgggca ggcggggctc gaggcggcgg ccgaggccgc ggaccgcggc gaatccgtca
     3841 tcgtcgtcga cgagggtcct gaggtcggcg gcgagctgct gtgtgatccg cagggcgccg
     3901 agacggcgcg tgccctgagc gaccgcgcgc gcgccgccgg cgcagagctc ctctcgcctg
     3961 cgacggcgat cgggatcttc gagcagaacc tcgttcccgt cgctcacggc aacctcctct
     4021 tgaagatccg cgcgacgcag gtcgtggtcg cgggcggtgt cgtcgagcag cccctcgtgt
     4081 tcccgggcaa cgatctcgcc ggcgtcgtgc tgccgtccgc ggtgcgccgg ctcgtcaact
     4141 actggtcgat ccttcccggc gagcgggctg ttgtcgtcac cgccgacgac cgaggactcc
     4201 gtgcggccca cgacctgcag agcgcgggcg ccgaggtgct cgaggtcgtc gacctccgac
     4261 agcagcaggt gacaaacctc gaggtgcagg gccggaaggg aagggtcagc cagatccatc
     4321 tgcgcgacac gtgggtcaag tgcgatttgg tcgtcatgtc cggcagcccg cagcccaaca
     4381 cgaagctgct cgcgcaggcg ggcgcccgcg tggagtacga cgagtcgcgc ggcatcttcg
     4441 tcccgtcgga gcttccggcg tctgtcgcgg ccgtcggctc ggtcacgggt gaggtcggcg
     4501 agccagccgt gccggcgccc atcctcggcc acctgggcga caagtgcttt gtctgcttct
     4561 gcgaagacca gacgacgaag gacctcaagt acgcgatcgc ggagggcttc tcctcgatcg
     4621 agctctccaa gcgctacacg acggtgacga tgggcccctg ccagggccgg ctctgcgccg
     4681 tcaacgcgat ccgcgtccac gcgaagcaga acggcgtcga cgagaacacg atcgggacga
     4741 cgaccgcacg cccgccgtac acgcccgtca ccctcggcct tctcgccggg cgcccgcacg
     4801 agcccgcgaa acgcacgtcg atccaccacc gtcacaagga tctgggcgcc gagatgatgt
     4861 gggcaggccc ctggcggcgc cccttcgcgt acggcgacga cccgggcgac gaggcgcaga
     4921 acgtccaccg ctcgctcggg ctgatcgacg tatcgacgct cggcaagctc ctcgtgcgcg
     4981 ggccggatgc cggtgccttc ctcgaccgcg tgtacccgaa ccggttctcg gacctgaagc
     5041 tcggccgcat ccgctacggc gtcctcacga ccgacgcggg gcggatcatg gatgacggca
     5101 ccgtcgcgcg actcggcgaa gagctctact acgtcaccac gacctcttcc ggggcgtccg
     5161 ccgtctacca gtggctgacg tggtggaacg cgatctggga aatggacgtc gacttcgtcg
     5221 acatcacggg cgcgatcgcc gccgtcaacg tggccggccc gaatgcccgc acattgatgg
     5281 aacgcgtcgc cgatatcgac atgtcgaacg aggcgttcac ctacttggac gccaagcact
     5341 gcggcgtcgc gggagtaccg acgctcgcgc tgcggatcgg ctttgtcggg gagctcggct
     5401 acgagctgca cttcccgagt ccgcacggcg aatacctctg ggacactctg ctggagcgcg
     5461 gtgacgatct gggagcacgt cccttcgggc tcgagccgca gcgcatcctg cggctcgaaa
     5521 agggccacgt gatcgtcggg caagacaccg attccgagtc gaacctgctc gaggcggcga
     5581 tgccctggat cttcaaagcc gacaagccag acttcgtggg caagtggggg acgcagcagg
     5641 ttcagttgcg agggctcgac tggatgctcg tcgggttcga gagtccgagc ggcgccgtcc
     5701 ccctcgaagg cggccaggtc ctcgtcgacg gtcagtcggc cgggcgtgtc acaagcgccc
     5761 gcttcagcgc agagttgggc aaggtgatcg gcctcgccgt catccccaac gagctggccg
     5821 tggacggcgg ggctttcgac gtacaggtcg acggccgaca cgtgccgatg cgggttcatc
     5881 tcgggccctt cttcgacccc gagggggagc gattgaaggc gtgagcgagc ttgcgttcat
     5941 ctcgcccggc cgtgccgtcg actatcgcga catgcatccc gtcgcccgct cgccgctcga
     6001 tcgccgccag cgcgacgccg gagcaacgtt cgaggagcgc gacggctggc tcgtaccggt
     6061 gtcgatgccc cacgagcagg agtacctcgg acgggtcggc atcgcggatc tctcatacct
     6121 gacgaaactc gaggttcgcc ccgcgggcga ggcgctcgag ggcgaggaca tcgtctggta
     6181 cgagatctcg ccgcggcgcg cgctcgtgtt ctgcaagcct tccgacgagg cgggtgtccg
     6241 ggagcagctc gggagtcggg caaccgtcga tgtcaccgcc gcattaggca tcctcatggt
     6301 tgccgggccc gaggctccga ctctgatccg gcggctcacg cacctccacc acttcccctc
     6361 gggcggcgac gtggcgcaca tcactgccca tgtgctcgag gaaggcggga tctattggat
     6421 cgtcttcccg caggaggaag gccactacct gtgggaggtc gcggtcgacc gcgccctgcc
     6481 gctcggcggc ggcccggtcg gggtcgacgc gctgggagcg acggcgtgaa ggacgtcttc
     6541 tttcgcaaag agattctgcg tacgccggcc gagctcaagc gtcactacga cgtcgtcatc
     6601 gtcggtggcg gctcgcacgg gctcgccact gcctactacc tcgccaccca gcacgggatc
     6661 acagacgtcg ccatcctcga gaagagctac atcggctcgg gcgccgcggg tcgcaacacc
     6721 acgatcatcc gctcgaatta ccgcacgccc gaaggggccg cgttctacaa ggagagcgtc
     6781 gccctctacg agaggctctc gaccgagctc gacttcaacc tcatgttctc gcagcacggg
     6841 catttcaccc ttgcgcactc ggaccgctcg ctggtcgttc agcaggagcg cgcggaggtc
     6901 aacaagctgc tcggcatcga cagccgcgtg atcgatgcag ctgaggtgaa ggagctctgc
     6961 ccgcagatca gcatctcgcg cgacgtcacc tggccgatcc tcggggccct ctaccacccg
     7021 ccgggcggca tcatccggca cgacgcggtc gtctggggct tcgcgaagca ggtcggccga
     7081 gccggaatcg acatccacca gggggtcgag gtcaccggct tcgacatccg cgggggcaag
     7141 gtgcacggcg tgcgcactac gcagggcgac atctcctgcc aggtcgcggt gagcgcgacc
     7201 gccggctggt cgagcatcgt cgccgacatg gcggggatcc cgctcccgat caccacgagc
     7261 atcctgcagg cattcgtcac cgagccggtg aagccgttcc tggacaagat catcgtctcg
     7321 tcccaaatgc acatctacgt ctcgcagacc gaccggggtg agttcctgat cggcgccgag
     7381 gtcgagccgt acccgaccta ctcgaacaag ggaacctttc cgttcgtgga gtacgcatgt
     7441 cgccacgcgc tcgagctctt tccgcagctg cacgcgacgc gaatccttcg ccactggacc
     7501 ggtctctgcg acatgagccc ggattacagc ccgatcatgg gggttaccga gatcgacggc
     7561 ttcatcgtcg actgcggctg gggcacctac ggcttcaaag ccgccccgat caccgggctg
     7621 acgctcgccg agctgatcgc gacgggcgag ccgccggagc tgatccggcc gttcacgctg
     7681 gagcgcttct ataccgacac gctcgtttcc gagctcgcgg ccgccgccgt gagccactga
     7741 cagagaacac gggaggagtt cggcacatgc tttcgagctt ggagatcgca caggccgcgt
     7801 cgctgcggcc catcaccgac gtggcagcgg acgccggcat cgagcccgac gagctcgacg
     7861 cctacggtcg ccacaaggcg aaagtcgatc tctcggttct cgaccggctc gccggcaagc
     7921 ccgacgcgaa gttgatcaac gtcaccgcga tcacgccgac gccggcgggc gagggaaaga
     7981 cgacgacgtc cgtgtcgttg acgcagggcc tcgggctcct cggtcgtaga ccggtgctct
     8041 gcttgcgcga ggcctcgctc gggccggtct tcgggatcaa gggcggcgcg gcgggcggcg
     8101 gctacgcaca ggttgggccg atggaggacc tgaacctcca cttcaccggc gacattcacg
     8161 ccatcaccgc cgcgaacaat cttctgtcgg cgatgatcga cgcccacctg ttgcacggaa
     8221 acgagctcgg gatcgatccg ctgtcgatct cgtggcgccg ctgtctcgac atgaacgacc
     8281 gctcgctgcg tgacgtcgcg acgggcctcg gcggacgcgt gaacggctac ccgcggcaga
     8341 cgggcttcga catcactgcg gcgagcgagg tcatgggtct cgtcgcggta gcgcgcgatc
     8401 tcgccgatct ccgtgagcgg ctcggcgcga tcacggtcgg cgccacgttc gaaggagagc
     8461 ccgtcacggc cgagcagatc aaggcggccg gctcgatggc ggtgcttctg aaagacgcga
     8521 tcaagccgaa cctcgttcag acgctcgagg ggcagcccgc cttcgtgcat tgcggcccgt
     8581 tcgcgaacat cgcgcacggc aacaactcgc tggtcgccga ccgcgtcgcg ctcaagctcg
     8641 gcgactacat gatcaccgag tcgggcttcg cctccgacat ggggatggag aagttcttcg
     8701 acatcacgtg ccggatgggc ggcctgcgtc ccagcgccgt cgtcctggtg gcgaccgtga
     8761 aggccctgaa gcatcacggc ggtgacatcg acggcggcct agcggagctc gagacgggcg
     8821 ccgccaacct cgcccgccac atcgggatca tcaagggctt cggcctggag gcggtggtcg
     8881 gcgtgaaccg cttcccgacc gacagcgacg aggagctcga gacggtacgg aggctcgcgc
     8941 tggagcacgg cgcccacgcg gccgagatca acgaggcgtt ccagcgaggc ggagaaggcg
     9001 cgcgggcact cgccgaagcg gtcgtcgacg ccgccgagca gccgaacgag ttcgacttcg
     9061 cctatccgag cgacgcgtcg atcgaggaga agatacgtgc catcgcgacg aaggtgtacg
     9121 gggccgacgg cgtcgagttg ctgccggcgg cgaagcaggc ggcggagcgg ttcgagcacg
     9181 cggggctggg cagtctcccc atctgcatgg cgaagaccca tctgtcgctc tcgcacgacc
     9241 cggccctcag gaacgctcca accgggttca ccgtgccggt gcgtgatcta cggccgtaca
     9301 ccggtgccgg ttggatcgtg gcgctctgcg gcgacatgat gacgatgccg ggcctcggca
     9361 aggcgcccgc cgccttcgcg atagacgtcg actcgaccgg ggacacggtc gggctcttct
     9421 agcggagtcc ccgacggggt agggtcacgg caaagccgaa tcgagacccg tggttccccc
     9481 gaggggagcg cggatccgtc tcgccaagct tctgggaaca tccccgctgg aaggaggttg
     9541 cggcatgagg attctcgtcg cgtacgacgg attcgagcag agccggcccg cgctggagga
     9601 ggcggttcgg atcgccctca cggtaagccc gggaatcgaa ggcgtgcgcg ctccgacgga
     9661 ggtcatcgtc ctcagcgtgg tgccgccgcc cgagcagatg ccgaaccccg gcggcgtcgc
     9721 cgggatgcgc ccccacgcgg tcgacgatgt cgccgaggcc catcgcttcc tcaaggagcg
     9781 gggcatcgag tcggaaatga agattacgca cgggaagcct gccgacgaga tcctccgcga
     9841 ggcgaccgag cgcaagtgcg accggatcgt catgggaacg agggggctgg ggaaaatggg
     9901 gcggctgctg cagggcagcg tcagcaccaa ggtcaccaag aacgcgccgt gcaccgtcgt
     9961 catcgccacc gccgggaaga tggagcggat cgagccgtcc ggctgaagag ctgagaggag
    10021 ctggcccgcg cggggacgac tccgagtgtc tcagctacgg ccccagggat ccttcttcgg
    10081 cgcgctcccg cttgcctccg cggggttcgc cgcgagcacc cgctgctgcc attcgttcgg
    10141 gtatttctcg aggcagaccg ggcacgcgtg gagctcgccg tgtggattcg tggggatgtg
    10201 accccaagag gagcgggaca ctatgccgtc gacagccttg acctggcata gaccgcagct
    10261 caggagaacc ggcatctccg aactataggg accgtgtccc tgcgacgacg accaagttca
    10321 cgctttcgat tgtc
//
LOCUS       DBNGNK010000389         6600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_710393,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000389 DBNGNK010000000
VERSION     DBNGNK010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_710393"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="AC150V_11505"
     CDS             <1..1205
                     /locus_tag="AC150V_11505"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007725498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR3763587.1"
                     /translation="LIASENFTWPAVLEAVGSVPTNKYAEGYPGRRYYGGCEVVDEME
                     TLAIDRAKELFGAEHANVQPHAGAQANMAVYMAALQPGDTILSLRLDHGGHLTHGLTV
                     NFSGRLYTIGHYGVSRETNVVDYDEVRALAVEHRPRLIVCGGSAYPRTVESWRFREIA
                     DEVGALLMTDMAHFSGLVAAGIHPNPVEHSDFVTSTTHKTLAGPRSGFILCRAEHAQA
                     VDRAVFPGTQGGPLMHAIAAKATCFKLAATHAFREYQLQVRKNADALAAGLTESGLDV
                     LTGGTDTHLLQLDLRRTDWTGKDAEERLGDVGITCNRNTVPYDERPPTVASGVRLGTP
                     APTMRGFDEDDFSEVARIIAGALSPAGDVTSLAARSAALCEKRPLYPGFRGYPSFRAD
                     GGAGIEAQ"
     gene            1202..1858
                     /gene="upp"
                     /locus_tag="AC150V_11510"
     CDS             1202..1858
                     /gene="upp"
                     /locus_tag="AC150V_11510"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR3763588.1"
                     /translation="MTSVHASVSAGTVVEVDHPLVQHKLALLRDVTTTTHMFRQLVNE
                     LTLLITYEATKELADEEVEIETPLEWTTARRISGKKVAVCPILRAGVGMLDGVLSLLP
                     GARVGFIGLYRNEETLQPVEYYVKLPSDVADRDVLLLDPMLATGNSTAAAVATLKEAG
                     ASSIRLIALIAAPEGIARIHADHPDVSIIVAAIDRGLNEKGYIVPGLGDAGDRLYGTK
                     "
     gene            1868..3013
                     /locus_tag="AC150V_11515"
     CDS             1868..3013
                     /locus_tag="AC150V_11515"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR3763589.1"
                     /translation="MLEQLGDHPEVLYGAAVAFVVVVLLTPAVGGMARLLGVVDRPDG
                     ARRLNRRPVPRLGGLAIFLGIIVPALAFLELSEQMRGILLGAAVACVVGAIDDFRGLA
                     PVPKLAGQILAAGIVVASGVWIDRFTFPVAGIVELPAYVGMPLTVLWIVAVMNMVNFL
                     DGMDGLAAGVCAISGVTFAVLALSLGKADAAILAAVVTGACLGFLRHNFFPARIFMGD
                     SGALVLGFVLASVSVAGLVKTASTVVLFLPLLVLAVPILDTSFVVAKRLKYRRPVMTA
                     DRSHLHHRFVDIGFSQRRAALTMWAWCGTLAAAALATNYIPFRAGGSWHLWETVAITA
                     IALVALAFSVYVVYLLEIVKLANPRVRRRLAERTASLDSELETRRSA"
     gene            3087..3392
                     /locus_tag="AC150V_11520"
     CDS             3087..3392
                     /locus_tag="AC150V_11520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR3763590.1"
                     /translation="MIVVRFKVRCQPGKARAVMDALADVIEPSRALAGVVSFDIAHDL
                     LDPNAFIAIEVFEDRAALDRQESLDEVGRALAVLGESLAEAPEATIFHVSASEPHGG"
     gene            complement(3406..4122)
                     /locus_tag="AC150V_11525"
     CDS             complement(3406..4122)
                     /locus_tag="AC150V_11525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3763591.1"
                     /translation="MERVVLVHGSVFGGRMTWAAQRRGLRDRFDLVVIERPGFPPNPP
                     VERVDFEQDAEVVCAALEPPSHLVGHSYGGVGCLLAAGRCAERVLSLTVLEPPALDVA
                     RGKPAADTFIREGEEWWATGPVDDPEAFLRGFLRYVGSGYDPPSPLPPDLERGATALI
                     DERPPWEARIPLERLAEASFPVLVVSGDHHAAFDAVCDVLEDRLGAQRAVLSGYGHVP
                     QSHPDFNDVLADFVEQSARP"
     gene            4214..4408
                     /locus_tag="AC150V_11530"
     CDS             4214..4408
                     /locus_tag="AC150V_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763592.1"
                     /translation="MGPHSLATASGLLLGTTGAAMVVGALLGWAAGSWGVGLLIGAVV
                     GIPLAVFVVYKVYSRAGVTA"
     gene            4405..4824
                     /locus_tag="AC150V_11535"
     CDS             4405..4824
                     /locus_tag="AC150V_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763593.1"
                     /translation="MTRAASDTRSRGLFSTPRPVPGRLLPALAGTVLIVLALPVFAIA
                     GWPLSGWALAAVLWVAGQGLALLLTHLHTRSGNLAASGVAGIGMSFRALIVGIPLVAV
                     TVADARVGLAAVVLYVLAFTLELAVGLVSYFGSETRT"
     gene            4821..5714
                     /gene="atpB"
                     /locus_tag="AC150V_11540"
     CDS             4821..5714
                     /gene="atpB"
                     /locus_tag="AC150V_11540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR3763594.1"
                     /translation="MKRLGALALALWLALPSLALAAEGAEEETKFDPAHEWEYKAWIP
                     IEIGPLDLSINKPVAYLLLGALLTCLLGIVTMRVKMRRTPDGPDRKQALGEQIYEIAQ
                     VQVAEQGLPSRAIGRWFPYVATLMLFIWVVNMIGFIPLPLSDEKFTIAGIELPTLAIF
                     AATSTLSVTLALALMTFVFTHVEGIRANGVWTYFKSWIPEVPKVMYPLIIPLEILGQF
                     MRLISLSVRLYANMLAGHMLILTFLGLIFVLESLALVVIAIPAATVFYLFEVVIVVSI
                     QAYIFAALSAIYIGSAIEPEH"
     gene            5746..6015
                     /locus_tag="AC150V_11545"
     CDS             5746..6015
                     /locus_tag="AC150V_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR3763595.1"
                     /translation="MLSSILLLAQTTGDVTDLGKAIALALGIGLGSLGAGIGIGIIFG
                     NMISAVARQPELRGELQGIQWLGFALTEAVVFYGLIGGLLAFVLV"
     gene            6020..6574
                     /gene="atpF"
                     /locus_tag="AC150V_11550"
     CDS             6020..6574
                     /gene="atpF"
                     /locus_tag="AC150V_11550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR3763596.1"
                     /translation="MSPFLLVAAEEEASGGLISVVPGLMIWTLITFAITFFVLKRYAF
                     GPIQKTIDTRRDRIAEAVEEADKARVEAAELLEQHRALIAEAKAESGEILTEARKVAD
                     AQMARAKEEVEEERQRRLEETRKQIAAETARAIDQIRSEVAGLTVEATEAVVGKVLDS
                     ADQRRLIAEAIEGLDFSALEQRRN"
BASE COUNT         1010 a         2270 c         2199 g         1121 t
ORIGIN      
        1 agctgatcgc atcggagaac ttcacgtggc cggcggtgct cgaggcggtc gggagcgttc
       61 ccacgaacaa gtacgccgag ggctacccgg gacgccggta ctacggcgga tgcgaagtcg
      121 tcgacgagat ggagacgctg gcgatcgacc gtgcgaagga gctgtttggc gccgagcacg
      181 ccaacgtgca gccgcacgcg ggcgcccagg cgaacatggc cgtctacatg gcggccttgc
      241 agccgggaga cacgatcttg tctctccgtc tcgaccacgg cgggcatttg acccacgggc
      301 tcacggtcaa cttctctggt cggctataca cgatcggcca ctacggcgtc tcccgcgaga
      361 ccaacgtcgt cgactacgac gaggttcggg cccttgcggt cgagcatcgt ccccgtctga
      421 tcgtctgcgg aggctcggcc tatccgcgca cggtggagtc gtggcgcttc cgagagattg
      481 ccgacgaggt cggcgccctg ctgatgacgg acatggcgca cttctcgggg ctcgtcgccg
      541 cggggattca tccaaacccc gtcgagcact ccgacttcgt cacctccacg acccacaaga
      601 cgctcgccgg cccgcgttcg gggttcatcc tctgtcgggc agagcacgcc caggcggtcg
      661 accgcgcggt attcccgggc acgcagggcg ggcctctcat gcacgcaatc gcagcaaagg
      721 cgacctgctt caaactcgcc gcgacccatg ccttccgtga gtaccagctg caggtgcgga
      781 agaacgcgga cgcgctcgcg gccggcttga cagagagcgg tctcgacgtg ctcacaggcg
      841 gcaccgacac ccatctgctg cagctcgacc tccgacgcac cgactggaca gggaaggacg
      901 ccgaggagcg cctaggcgac gtcggaatca cctgcaatcg caacacggtg ccctacgacg
      961 agcggccgcc cacagtcgca tccggcgtcc gcctgggaac acccgctccg acgatgcgag
     1021 gcttcgacga ggacgatttc agcgaggtgg cgaggatcat cgccggcgcg ctctccccgg
     1081 caggcgacgt cacgtcgctg gccgcccgca gcgccgcgct ctgcgagaag cggccgctct
     1141 atcccggatt ccgcggctac ccgtccttcc gcgccgacgg gggcgccgga atcgaggcac
     1201 aatgacctcc gtgcacgcgt ccgtctccgc cggaacggtc gtcgaggtcg accatccgct
     1261 ggttcagcac aagctggcgc tcctccgaga cgtcacgacg acgacacaca tgttccggca
     1321 gctcgtcaac gagctgacgt tgctgatcac ttacgaggcg acgaaggagc tcgccgacga
     1381 ggaggtcgag atcgagacgc cgctcgagtg gacgacggcg agaaggatct ccggcaagaa
     1441 ggtcgccgtc tgccccatcc tgcgcgccgg cgtcgggatg ctcgacggcg tcctgtcact
     1501 tcttccgggc gcacgcgtcg gcttcatcgg gctctaccgg aacgaagaga cgctgcagcc
     1561 cgtcgagtac tacgtgaagc ttccgagcga cgtcgccgat cgtgacgtcc tgttgctgga
     1621 cccgatgctg gcaaccggga actcgaccgc ggcagccgtc gcgacgctca aggaggcggg
     1681 cgccagctcg atccggttga tcgcgctgat cgccgcccca gagggcatcg cccgaatcca
     1741 cgccgatcac ccggacgtct cgatcatcgt cgccgccatc gaccggggcc tcaacgagaa
     1801 gggctacatc gtgcccgggc tcggcgacgc aggcgacagg ctgtacggga cgaagtaggc
     1861 cttcgtcatg ctcgagcagc tcggcgatca ccccgaagtt ctctacgggg ctgccgtcgc
     1921 gttcgtcgtc gtcgtgctgc tcacaccggc ggtcggcggc atggcccggc tcctcggtgt
     1981 cgtagaccgg cccgacggcg ctcgccggct caaccgccgt ccggtgccgc gtctcggcgg
     2041 gcttgcgatt ttcctcggca tcatcgtccc cgcgctcgcg tttctcgagc tgtcggagca
     2101 gatgcggggg atcctgctcg gggccgcggt cgcgtgcgtc gtcggcgcga tcgacgactt
     2161 ccgcggtctc gccccggtgc cgaagctcgc cggacagatc ctcgcagcgg gaatcgtcgt
     2221 tgcctcgggc gtctggatcg accgcttcac gttccccgtc gcgggaatcg tcgagctgcc
     2281 ggcgtacgtc ggcatgcctc tgaccgtcct ctggatcgtg gccgtcatga acatggtcaa
     2341 cttcctcgac ggcatggacg gcctcgcggc cggagtgtgc gcgatctcgg gtgtcacctt
     2401 cgccgtattg gcactctcgc tcggcaaggc ggacgccgcg atcctggctg cggtcgtgac
     2461 cggagcctgt ctcggcttcc tccggcacaa tttcttcccc gcacgcatct tcatgggcga
     2521 ctccggtgcg ctcgtgctcg gcttcgtgct ggcctctgtc tccgtcgccg gactcgtgaa
     2581 gacggcgtcg accgtcgtcc tgttcctgcc gctcctggtg ctcgcggtgc cgatcctcga
     2641 cacgtccttc gtcgtcgcga agcggctcaa gtaccggcgc cccgtgatga ccgcagaccg
     2701 ctcccatctc catcatcgct tcgtcgacat cggcttcagc cagcgccggg ccgctctcac
     2761 gatgtgggcc tggtgcggca ccctggcggc ggccgcactc gcgacgaact acatcccgtt
     2821 ccgggcaggc ggaagctggc atctgtggga gacggttgcc atcaccgcca tcgcgctcgt
     2881 cgccctcgcc ttctcggtgt acgtcgtcta cctgctcgag atcgtcaagc tcgccaaccc
     2941 gcgcgtgcgg cgtcggctcg cggagcgcac ggcgagcctc gattcggagt tggagacacg
     3001 gcgctcggca tgaacgcgac cgagagcgtc ggcgttgacg ggagtggggg gccggcggcg
     3061 acctcaaggc tgctggggac acggcgatga tcgtcgtccg tttcaaggtg cgatgccagc
     3121 cgggcaaggc tcgggcggtg atggatgccc tcgcggacgt catcgagccg agccgagcgc
     3181 tcgccggggt cgtcagcttc gacatcgcgc acgacctcct cgacccgaat gcgttcatcg
     3241 ccatcgaggt cttcgaggat cgggccgcac tcgaccgcca ggagtcgctg gatgaagtcg
     3301 gaagggcact cgccgtgctc ggcgagtccc tcgccgaggc gcccgaggcg acgatcttcc
     3361 acgtttcggc ctccgagccg cacggaggct gacggccgaa tccggctacg ggcgtgccga
     3421 ctgctcgacg aagtcggcga gcacgtcgtt gaagtccggg tgcgactggg ggacgtgccc
     3481 gtatccggac aagacggccc tctgcgcgcc gaggcggtcc tcgagcacgt cgcaaacggc
     3541 gtcgaacgcg gcgtggtggt ctccggagac gacgagtacg ggaaacgatg cctcggcgag
     3601 cctctcgagg gggattcgcg cctcccacgg cggtcgctcg tcgatcaaag ctgtcgcgcc
     3661 ccgctccagg tcgggtggca ggggcgaggg tgggtcgtag cccgaaccga cgtagcggag
     3721 aaagcccctc aggaacgcct ccgggtcgtc gaccggtccg gttgcccacc actcctcgcc
     3781 ctcgcggatg aacgtgtcgg cagcgggctt gccacgcgcg acgtccagcg cgggcggctc
     3841 gagcacggtg agcgacagca cacgctccgc gcagcgccca gccgcaagca ggcagccgac
     3901 gccgccgtag gagtggccga cgaggtggga gggaggctcg agagcggcgc agacgacctc
     3961 ggcgtcctgt tcgaagtcga ctcgctcgac gggcgggttg ggcgggaagc ccgggcgctc
     4021 gatgacgacg aggtcgaagc ggtcacggag tccgcggcgc tgtgcagccc acgtcatccg
     4081 gcccccgaag acgctgccgt gcacgagcac cacgcgctcc acgaaggcat cctagtcgcg
     4141 taacgaagtg ccctcgattc cgcacgaaac gtggaatcag actgttacaa ggtggtggta
     4201 tgctccgctc cggatgggac cgcactccct cgcgacggcc tccgggctcc tgctcggtac
     4261 gacgggtgct gccatggtcg tcggcgcgct cctcggctgg gccgccggaa gctggggggt
     4321 cggcctgctg atcggcgccg ttgtcggcat cccgcttgcg gtcttcgtcg tgtacaaggt
     4381 gtattcccgg gcaggagtga cggcgtgact cgggccgcct ccgatacgcg ctcgcgggga
     4441 ctcttctcga ctccgcgccc ggttcccggc aggctcctgc ccgcgctcgc cggaactgtt
     4501 ctgatcgtgc tcgcgctgcc cgtgttcgcg atcgccggct ggcccctctc cggctgggcg
     4561 ctcgcggccg ttctctgggt cgccggccag gggctcgcgc tcctactgac gcacctccac
     4621 acgcgtagcg gtaacctcgc cgcctcaggc gtggcgggca tcggaatgag ctttcgtgcg
     4681 ttgatcgtcg gtataccgct cgtcgcggtg acggttgcgg acgcgcgggt cggcctggca
     4741 gcggtggttc tctacgtgct cgcattcacg ctggagctcg cggtcggtct cgtgagctac
     4801 ttcggctcgg agacgcggac gtgaagaggc tcggcgcgct cgctctcgcc ctgtggctcg
     4861 cgctgccgtc gctcgcgctc gcagccgagg gcgccgagga ggagacgaaa ttcgatccgg
     4921 cccacgagtg ggagtacaag gcatggatac cgatcgagat cgggccgctc gacctctcga
     4981 tcaacaagcc cgttgcctat ctgctcctcg gcgccctcct gacctgcctg ctgggcatcg
     5041 tcacgatgcg cgtcaagatg cggcgtacgc ccgacgggcc cgaccgcaag caggcgctcg
     5101 gagagcagat ctacgaaatc gcccaggtac aggtcgccga gcaggggctg ccgtcgaggg
     5161 cgatcggacg ctggttcccc tacgtcgcga ccctgatgct cttcatctgg gtcgtcaaca
     5221 tgatcggctt catcccgcta ccgctctcgg acgagaagtt cacgatcgcc gggatcgagc
     5281 tgccgacgct cgccatcttc gccgcgacct cgacgctctc cgtcacgctc gccctcgcgc
     5341 tgatgacgtt cgtcttcacg cacgtcgaag ggatccgtgc caacggtgtc tggacgtact
     5401 tcaagagctg gatccccgag gtgccgaagg tgatgtaccc gctcatcatt ccgctcgaga
     5461 tcctggggca gttcatgcgg ctcatcagcc tctcggtccg gctctacgcg aacatgctcg
     5521 ccgggcacat gctgatcctc accttcctcg ggctcatctt cgtgctcgag agcctggccc
     5581 tggtcgtgat cgccattccg gccgcgacgg tcttctacct cttcgaggtc gtgatcgtcg
     5641 tctccatcca ggcatatatc ttcgctgccc tgtccgccat ctacatcggc tcggcgatcg
     5701 agccggaaca ctgacctgta ggaacgaagg agggaacccg tcccaatgct gtcgtccatc
     5761 ctcttgctcg ctcagaccac gggcgacgtc accgacctcg gaaaggccat cgcgctcgcg
     5821 ctcggcatcg gcctcggctc gctcggcgca ggcatcggca tcggcatcat cttcggcaac
     5881 atgatctcgg cggtcgcgcg ccagcccgag ctccgcggtg agctccaggg catccagtgg
     5941 ctcggcttcg cactcaccga ggcagtcgtc ttctacggcc tcatcggcgg cctgctcgcc
     6001 ttcgtcctcg tctgagcaga tgtcgccgtt cttgctcgtc gccgcagaag aagaggcctc
     6061 cgggggtctg atctcggtcg tccccgggct catgatctgg acgctgatca cgttcgcgat
     6121 cacgttcttc gtactcaagc ggtacgcgtt cggaccgatc cagaagacca tcgacacgcg
     6181 ccgcgatcgg atcgccgagg ccgtcgagga ggcggacaaa gcacgagtcg aggctgccga
     6241 gttgctcgag cagcatcgtg cgttgatcgc cgaggccaag gccgagtcgg gagagatcct
     6301 caccgaggcc cgcaaggtgg cagacgccca gatggcgcgg gcgaaagagg aggtcgagga
     6361 ggagcgccag cggaggctcg aggagacccg caagcagatc gccgccgaga cggctcgtgc
     6421 catcgaccag atccgctccg aggtcgccgg gctgaccgtc gaggcgaccg aggcggtcgt
     6481 cggcaaggtg ctcgacagcg cggatcaacg gcgtctgatc gccgaggcga tcgaggggct
     6541 cgacttctcg gcgctggagc agcggaggaa ctaggggccg ccgtgccgat cgctgaacgc
//
LOCUS       DBNGNK010000390        14402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1930405,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000390 DBNGNK010000000
VERSION     DBNGNK010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1930405"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /gene="narI"
                     /locus_tag="AC150V_11555"
     CDS             <1..520
                     /gene="narI"
                     /locus_tag="AC150V_11555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR3763597.1"
                     /translation="ILGILIPRSWTEAVGIDDRIYHWLAVVAGGAAGLAVLGGFAILV
                     FRRLRIDRVRATTLRSDNILYPLLALTIVTGVLATFWGSAVDDYAYRETVSPYFRGIF
                     SFRPDWQLMLNAPLIFRLHVVSAFVLYAVWPFSRLVHVWSVPVTYLGRAQILYRSREG
                     RVAVDRRSAARN"
     gene            complement(523..981)
                     /locus_tag="AC150V_11560"
     CDS             complement(523..981)
                     /locus_tag="AC150V_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR3763598.1"
                     /translation="MKRHPALVPLSHDHHHGLVQARRLRTAGDSGDGRRCAEQARAFL
                     RFYNDETLGHFREEEELLFPLALREGEEPPELLVQALVDHVRIHRHVAELRAAVEAGS
                     VDPELVSAVGSRLEAHIRLEERELFPLIEEIAGGDELASLSIPPREQPLV"
     gene            complement(1032..1763)
                     /gene="cofE"
                     /locus_tag="AC150V_11565"
     CDS             complement(1032..1763)
                     /gene="cofE"
                     /locus_tag="AC150V_11565"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR3763599.1"
                     /translation="MTPLRGIPELADGDDLAAEIAAAAERAGGLREHDVVVVAQKAVS
                     KVEGRVVSLDAIEPSPRAIERAGPEGDARQVEVILRESEEVVRSRPPLVIARTRHGFV
                     CASAGVDASNAPAPETVVLLPDDPDASAARLREGLVSLTGIDVGVIVSDSFGRAWRQG
                     TTDVALGVAGLAALRDLRGSSDGTGHHMLRTTQIAVADEIAGAAELVMGKAGGIPAAV
                     VRGVSAAGQGSGTDLVMPRDRDMFS"
     gene            complement(1778..2398)
                     /locus_tag="AC150V_11570"
     CDS             complement(1778..2398)
                     /locus_tag="AC150V_11570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR3763600.1"
                     /translation="MKIALIGGTGSFGKALAVRLREAGHDVVIGSRDAERAAEVAAGL
                     GVSGATNAEAAASAEFVVLTTNADGAVDTARALREAIGSTPVLSVAAELEFTKSGVRP
                     TAHATSIAQRIQDELAGPVVAGLHSLAAANVGGDEAPDEDALVCGDDPEAKAAALEVA
                     TAITGGRVLDAGPLASARALEGLTAVIVNVNKRYKAHAGIRFTGLN"
     gene            complement(2395..3306)
                     /gene="cofD"
                     /locus_tag="AC150V_11575"
     CDS             complement(2395..3306)
                     /gene="cofD"
                     /locus_tag="AC150V_11575"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR3763601.1"
                     /translation="MKVALLSGGVGGARFARGLVQVLAPGELTIIGNVGDDVEVMGLH
                     VSPDLDSLLYALAGLNDETRGWGRAGETWNALDAASAWGGEDWFALGDLDIGLHLVRT
                     HALRSGETLSAVTKRLCERANLPADLVPATDDRLRTLVHTPAGVFGFQRWFVQRGHAD
                     DVDGLSFEGAAAARAVPRGLDALAAADVILIAPSNPYVSIRPILAVRELAEALERRHV
                     PCLAVSPLVGGEAVKGPAGRMLSRMAGGTEPRHLAALYEGLIDVLVIDERDMPAAAAV
                     SLVATQTLMTDADASRRLAEVVLEAAA"
     gene            complement(3303..3887)
                     /locus_tag="AC150V_11580"
     CDS             complement(3303..3887)
                     /locus_tag="AC150V_11580"
                     /inference="COORDINATES: protein motif:HMM:NF014085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HIR3763602.1"
                     /translation="MLSIVVPFRAGGKTRLPQEIRRDASLAMLGDVVESALAVANTWV
                     VTSDEAAGYVAARQGARILDDPGHGQGAAVAAALAGGSGAVLVVNADVPRVRPSDLNA
                     LAFPARAGAMALVEARDGTTNALALPAPEAFAPLYGPGSAAQFRAHAEALGLVHHDLA
                     LRNLQDDVDTLADLERIGRRAGTRTRAVLLAVTG"
     gene            complement(4065..4355)
                     /locus_tag="AC150V_11585"
     CDS             complement(4065..4355)
                     /locus_tag="AC150V_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763603.1"
                     /translation="MSYKTCPEWPELMELAPDLQFKHMSVAAAQLPVEVLTTVSHVSL
                     DVVEICCDQEHHVYNAAHTDPEVAAALRDTQSHWFEVNEWATSGPGHSSHAA"
     gene            complement(4352..5557)
                     /locus_tag="AC150V_11590"
     CDS             complement(4352..5557)
                     /locus_tag="AC150V_11590"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763604.1"
                     /translation="MRRGAISVDGVSRRFVVRARDTRTLKELFVARGRTGGEEVWALR
                     DVSLDIAPGEAVGLIGRNGSGKTTLLRVIAEIIRPTKGRIAVGGRVGSLLELGAGFHP
                     DFTGRENVYLNGSIQGLRRAEIRERFDEIVSFAELEHAIDRPVRTYSSGMYMRLGFAI
                     AAFLDADILLLDEVFAVGDEAFQRKCFGRIFEFKQGGGTIVFVSHDAGAVERLCDRAV
                     LLQVGSVAYDGPAHEAIVRYRRQLAEETDPEERGAGLTEWGTGEATITAAVLSGADGA
                     AREQFLAGERLQLHVRLEAHESVAAPRLQLELRDDSGLLVAADDVDTAGLGWDGGEGL
                     GLLLDVPELPLSFGRFHLRLGLTDATGERLLHWLDDALVFLVYPDGAGRGLVRLEGKW
                     TADTNENVR"
     gene            complement(5562..6029)
                     /locus_tag="AC150V_11595"
     CDS             complement(5562..6029)
                     /locus_tag="AC150V_11595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3763605.1"
                     /translation="MSVRLRALGDDELSGWLADSQAGYADDMVRNARLSPADAERKAE
                     EDFANLLPEGLATPGAALYAIEADGEVIGSLFFAERSRDGRRVGWLYEIRVDEELRGR
                     GLGRRAMELFEDEARARGLDRFELNVFGGSDVARRLYRSRGFDEIAVTMGKDL"
     gene            complement(6029..7057)
                     /gene="hemE"
                     /locus_tag="AC150V_11600"
     CDS             complement(6029..7057)
                     /gene="hemE"
                     /locus_tag="AC150V_11600"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016576822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR3763606.1"
                     /translation="MAASLLVRAARGEPVERTPIWFMRQAGRSLSEYRAIRERHSFFE
                     VAHTPELCAEVTLQPVRRHDVDAAVLFADIMTPVLGMGVNVELVEGVGPVVDPPIRSA
                     ADVARLRVPEPEEAFAPVLEAIRLVRAELPEEKAVLGFCGGPFTVAGYLVEGRPSREL
                     SRTKALMLGEPELWGALLERLSDVFAAYVEAKAGAGADAIQLFDSWAGALSPDHYRQY
                     VAPWSSAVLRAVDVPTIHFGTGTATLLPAMAAAGGDVIGLDWRIPLDEGRALVGGGPV
                     QGNLDPAVLLADWDVVEREALEVLDRAGGTGHIFNLGHGVLPATNPESLTRLVELVRE
                     RTEAPAAA"
     gene            7079..7903
                     /locus_tag="AC150V_11605"
     CDS             7079..7903
                     /locus_tag="AC150V_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763607.1"
                     /translation="MEPVGEHPVPAGPLAPRWLAHDVGSPRAGVLGSARLRLENAGTA
                     TWRSRGKLGMQLAYHWLDPLGNPIVWDGIRTALPRPIEPGETIELDAPLLAPRPPGSY
                     VLAFDFLEEGHFWFEELGIRRLELPIAVAPRISERRLAVVVHGGVDPETDAALEAQEE
                     SLVDEGAVATAHLVGGAVPAPDWSRRVLDLHAEGYAAVGGAVEPEERRLRKRLKPWAP
                     GGGRSPGFGHPLLLPSLLAGEEPGEHEGLPAFEGEDALFDGRIVIRLRRRSGRRPA"
     gene            complement(7866..8666)
                     /locus_tag="AC150V_11610"
     CDS             complement(7866..8666)
                     /locus_tag="AC150V_11610"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763608.1"
                     /translation="MSVYADLFRYRELFGSLFRRDLRAKYKGSVLGLAWSLAHPLMLM
                     AVYLVVFSVLWKATATDFDNYWLFLLCGLPAWVFFATSLQSSSRSLLENVNLIRKVRF
                     PRQLVPLSTVATQLVSFVVMLAIVIVLSLAFQADARGGVWMAVPVAVVIVAFVGGFSL
                     MVASANAAFRDVEHIVAALLLPWFFLTPILYRLEDLPGAAAHPWLVDLLHWGNPLTPA
                     IESLRTPLFYGDAPPTADFVYLCVATVVFMLLGALVFRQVDDRIAAEA"
     gene            8725..10542
                     /locus_tag="AC150V_11615"
     CDS             8725..10542
                     /locus_tag="AC150V_11615"
                     /inference="COORDINATES: protein motif:HMM:NF025104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR3763609.1"
                     /translation="MEAAIAIAALPVSALVLWALLRTSAGRRLVAVPTGERWHARATP
                     TFGGLGIYAGLAAGIGLAVATGAVEASSELAGVAAGASLLFVAGVVDDVRHLSPIVKL
                     GAQFAAAGIAIASGLTVELVGNDILATIIALVWLVGITNAFNLLDNMDGLAATLATIA
                     CGYFAIAAATDDPVNQLVLVIALALGAACLGFLPFNLRPGGAAAVFMGDSGSQVIGFT
                     LASLALASSWTVAGTTAATVLLPLLVLAIPILDTSLVTIVRLIERRPVTQGGTDHTSH
                     RLVYYGLSEGKAVGLLALIAIALGATAVAYNILDNARITAIGVLVTFAILVQFASFLS
                     DLEERQRSGETGEEPSLLQALFSQPRRLLEILLDFVLICASFLASYLLIVGGEGGEWQ
                     RGTFLAALPILLGLRFLAFTALGVYRRVWRFAGPVDLAMIALGCVLSGAAALGVLVAI
                     RPLDDFPWQVFVLDAVLCSVVVAGSRLIFRAAALFHARRSVETRARVLVVGAGRFGRS
                     VARELQETPGTRVVGFLDDNPAVRRRRVRGVMVIGGTHEIERALERAHPDEVLVTIPD
                     APGDRLDRIVEASRAAGVPCRLVRRQTEVPPTLVETNVS"
     gene            complement(12419..13270)
                     /locus_tag="AC150V_11620"
     CDS             complement(12419..13270)
                     /locus_tag="AC150V_11620"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763610.1"
                     /translation="MTKADQYSRKAAGWTDQQYADADAYLEHRAALVIALGRRLEARS
                     EVLDLACGDGGLGERLMARGLSYRGVDATPEMVAAARTRLGDAVPLELGDLNDYAPPR
                     PVAATTLFRALYYARDRRSFFRRVRSYTAVKLVFDLNPRQYPVEEVLDDLYAAGFVDV
                     VVRPFFVPQNVALPRLLAALLRGLERSGPVARVLLRFRFSYLVAASIGTTSNATTAPS
                     SRRSLRLESSSAGTKVGPIRTSMRHGPGGGRKTTGFTSQALMTNRSSSVTRPIAGCLS
                     TTAAVPI"
     gene            complement(13267..14025)
                     /locus_tag="AC150V_11625"
     CDS             complement(13267..14025)
                     /locus_tag="AC150V_11625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3763611.1"
                     /translation="MSVSAVVVSHGHAAELTRLLPALAPQVDELVVIANVEGSVGTVA
                     PGTRVLANARPRTFAANVNQGVAATGGEFVLVANPDAVPEVGAVAVLNRFADEHPRCG
                     IAGPQLLWPDGTWQPSRRRFPTVRGTLERRTPLRLLRPPYQRQVEHYHLDERPERPVR
                     ADWLLGAFLLQRREMLEEIGGWDAGYRHYVEDIDLCYRAAQAGWERWYVPEAVVRHAY
                     AAVIDHRFLSRHTLWHARGMARFIRKHPERLGAL"
     gene            complement(14022..>14402)
                     /locus_tag="AC150V_11630"
     CDS             complement(14022..>14402)
                     /locus_tag="AC150V_11630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR3763612.1"
                     /translation="KDSALAAQLRRGGARLLGYVDDERLAALYREAVSLVQASRYEGF
                     GLPVLEAMACGCPVVTVPEPALLEVAGDAAVVVAEAALGEGIGRAMAERESLVAAGLE
                     RARAFSWQSTAERTVAAYREVLGA"
BASE COUNT         2174 a         5153 c         4921 g         2154 t
ORIGIN      
        1 catcctcggc atcctcattc cccgctcctg gacggaggcc gtcggaatcg acgaccgcat
       61 ctatcactgg ctcgcggtgg tggcgggcgg agcggcgggc ctggccgtcc tcggcggctt
      121 cgcgatcctc gtgttccgtc gcctgcgcat cgaccgagtc cgtgcgacga cgctccggag
      181 cgacaacatc ctctacccct tgcttgcgct caccatcgtc acgggagtgc tggcgacctt
      241 ctggggctcg gccgtcgacg actacgccta ccgggagacg gtcagcccgt acttccgggg
      301 catcttctcc ttccggcccg actggcagct catgctgaac gctcccctca tcttccgact
      361 gcacgtggtg tcggccttcg tgctgtacgc agtctggccg ttctcgcgac tcgtgcacgt
      421 ctggagcgtg cccgtcacct atctcggtcg agcgcagatc ctctaccgca gccgcgaggg
      481 tcgagtcgcc gtcgaccggc gctcggccgc tcgcaactga cctcacacga gcggctgctc
      541 tctaggcggg atcgagagcg aagcgagctc atcgcctccc gcgatctcct caatgagcgg
      601 gaagagctct cgctcctcga gacggatgtg cgcttcgagc cggctaccga cggccgagac
      661 gagctccggg tcgaccgatc cggcttcgac cgctgctcgg agctctgcga cgtgccggtg
      721 gatcctgacg tggtcgacga gcgcttgaac gagcagctcg ggcggctcct cgccctctcg
      781 tagagcgagg ggaaagagca gctcctcctc ctcacggaag tggccgagcg tctcgtcgtt
      841 gtagaagcga aggaacgccc gagcctgctc cgcgcatcgc cgcccgtccc cggaatcgcc
      901 ggccgtccgg agccgacgag cctgaacaag gccgtggtga tgatcgtgcg aaagcgggac
      961 gagggccgga tgtcgcttca cggagcgagt ctagagcggg ccggccgact gtcggaggtc
     1021 gagccgagcg ctcagctgaa catgtcgcgg tcgcgaggca tgacgaggtc ggtaccgctg
     1081 ccctggccgg cggccgagac gccgcgcacg acggccgccg gtatgccgcc cgcctttccc
     1141 atcacgagtt cggcggcgcc ggcaatctca tccgcgactg ctatctgcgt cgtgcggagc
     1201 atgtgatgcc ccgtgccgtc actgctgccg cgcaggtcgc ggagcgcggc gagaccggcg
     1261 acaccgagcg ccacatcggt cgtcccctgg cgccaggcac ggccgaacga gtcgctgacg
     1321 atcacgccga cgtcgatgcc tgtcaggctc acgaggcctt cgcggagccg cgcggcggag
     1381 gcgtccgggt cgtcggggag gagcacgact gtctccggcg cgggggcgtt cgatgcatcg
     1441 acgccggccg aggcgcagac gaagccgtgg cgggtccgcg cgatgaccaa cggcggccgt
     1501 gagcgcacca cctcttcgct ctctcgcaag atcacctcga cctggcgcgc atcgccctcg
     1561 gggccggcac gctcgatcgc gcgcggcgag ggttcgatcg cgtccagact gaccacgcgg
     1621 ccctcgacct tggagacggc cttctgcgcg acgacgacca cgtcatgctc gcgcaggccg
     1681 ccggcgcgct ccgccgcagc cgcgatctcg gcggcgaggt cgtccccgtc tgcgagctcg
     1741 gggatcccgc gcagcggggt cactcgcacc tcgccggtca attgagcccg gtgaaccgga
     1801 tcccggcgtg tgccttgtac cgcttgttca cgttgacgat tacggcggtg aggccctcga
     1861 gcgcccgcgc gctcgcgagc ggccctgcgt cgaggacacg accgccggtg atcgccgtcg
     1921 cgacctcgag cgcggcggcc tttgcctccg gatcgtctcc gcacaccagc gcatcctcgt
     1981 ccggtgcctc gtcgccgccg acgttcgccg cggcgagcga atggagaccc gctacgaccg
     2041 gccccgccag ctcgtcctga atgcgctgcg cgatcgacgt cgcgtgggca gtggggcgga
     2101 cgccgctctt cgtgaactcg agctcggcgg cgaccgagag caccggcgtg gagccgatcg
     2161 cctcgcggag cgcgcgggcg gtatcgacgg cgccgtccgc gttggtcgtg aggacgacga
     2221 actcggcgct cgcggcagct tcggcgttcg tggcacccga gaccccgagg ccggctgcga
     2281 cctccgccgc ccgctcggcg tcgcgcgagc cgatcacgac atcatgccca gcctcgcgga
     2341 ggcgaaccgc gagtgccttg ccgaagctcc cggtgccacc gatgagcgcg attttcacgc
     2401 ggcagcctcc aggacgacct cggccaggcg tcgcgacgcg tccgcatcgg tcatcaacgt
     2461 ctgcgtggcc accagcgata cggcagccgc cgcaggcatg tctctctcgt cgatcaccag
     2521 gacgtcgatc agcccctcgt acagggctgc aaggtgacgt ggctcagtgc cgccggccat
     2581 gcgcgagagc atacggccag cgggcccctt gacggcttca ccgcccacca gcgggctgac
     2641 agcgaggcag ggaacatgcc gccgctcgag cgcttcggcg agctcgcgaa cggcgaggat
     2701 cggccggatg ctgacatagg ggttgctggg agcgatcagg atgacgtcgg ccgcggccag
     2761 ggcgtcgagt ccccgaggca cggcgcgagc cgccgctgca ccctcgaaag agagaccgtc
     2821 gacgtcgtcg gcgtggccgc gctgcacgaa ccaccgctgg aagccgaaga cgcccgccgg
     2881 cgtgtggacg agcgtgcgca gtcgatcgtc tgtcgcggga acgagatcgg ccgggaggtt
     2941 tgcccgctcg cagagccgct tggtgaccgc tgaaagcgtt tcccccgacc gcagcgcgtg
     3001 cgtccggacg aggtggagcc cgatgtcgag atcaccgagc gcgaaccagt cctcgccgcc
     3061 ccacgcactc gcggcgtcga gagcattcca cgtctctccc gctcggcccc aaccgcgggt
     3121 ctcgtcgttc aggcccgcga gggcgtagag cagtgagtcg agatccggcg agacgtggag
     3181 ccccatgacc tcgacgtcgt ccccgacgtt gccgatgatc gtcagctcgc cgggagcgag
     3241 cacttggacc agacctcgtg cgaaacgtgc gccgccgacg ccgccggaca ggagagcgac
     3301 tttcatcccg ttacggcgag aaggactgcg cgggtgcggg ttccggcgcg gcgcccgatc
     3361 cgctccaggt cggccagcgt gtcgacgtcg tcctggaggt tgcgcagagc gaggtcgtgg
     3421 tgaacgagac cgagcgcttc ggcatgcgct ctgaattgcg cggcgctgcc cggcccgtag
     3481 agcggcgcga aggcttccgg cgcgggtagc gccagggcgt tcgtcgtccc gtcccgcgct
     3541 tcgacgagcg ccatggcacc ggcgcgagcg gggaaggcga gggcgttcag gtcggatggg
     3601 cggacgcgcg gaacgtcggc gttgacgacg agaaccgcgc cggagccacc tgcgagggca
     3661 gccgcgacgg ccgcgccctg cccgtgaccc gggtcgtcga ggatgcgggc gccctgccgc
     3721 gcggcgacgt acccagccgc ttcgtcggac gtgacgaccc aggtgttcgc caccgccagc
     3781 gctgattcga cgacgtcgcc gagcatcgcc agcgacgcgt cacggcggat ctcctgcggc
     3841 agccgggtct tccccccggc gcgaaagggc acgacgatcg acagcacccc gacagcctac
     3901 gctcccgccg cgtccagtca cctgggggca ggaagtcaca caattgttgc ggttcgcccg
     3961 cggccgaacc ggccatgtca ggggacagtc cctccgtttc cggccggttc tggtggcggc
     4021 cgttcaggcg gcgcgcgggc cgtcagcgac gccgcatgat ggcctcaggc ggcgtgagac
     4081 gagtgaccgg gccccgaggt cgcccactcg ttgacctcga accagtgcga ctgcgtgtcg
     4141 cgaagagcgg ccgcgacctc agggtcggtg tgcgccgcgt tgtagacgtg gtgctcctga
     4201 tcgcagcaga tctcgacaac gtcgagcgag acgtggctca ccgtcgtcaa cacctcgacc
     4261 ggcagctgcg ccgccgcgac gctcatgtgc ttgaactgga gatcgggcgc gagctccatc
     4321 agctcgggcc actccggaca ggtcttgtag ctcatcggac gttctcgttc gtgtccgccg
     4381 tccacttccc ttcgagccgg acgagtccgc gcccggctcc atcagggtag acgaggaaga
     4441 ccaacgcatc gtccagccag tggagcagcc tttcgccggt cgcgtcggtc agaccgaggc
     4501 ggaggtggaa gcggccgaag gacagaggca gttctggtac gtcgagcagc aaaccgaggc
     4561 cctcgccacc gtcccagccc agccctgcag tgtcgacgtc gtcggcggcc acgagcagcc
     4621 ccgagtcgtc gcgaagctcg agctgcaggc gcggcgccgc aacggactcg tgcgcttcca
     4681 gcctgacatg gagctgcagc cgctctccgg ctaagaactg ctcgcgtgcc gcaccgtctg
     4741 ccccggacaa cactgccgcc gtgatcgtcg cctcgcccgt gccccactcg gtgagcccgg
     4801 cgcctcgctc ctcgggatct gtctcctccg ctagctgcct gcggtatcgc acgatcgcct
     4861 cgtgcgcggg gccgtcgtag gcgacgctcc ccacctgcag gagtaccgcg cggtcgcaca
     4921 accgctcgac ggcgcccgcg tcatgggaga cgaagacgat ggtgccgccg ccctgcttga
     4981 actcgaagat acgcccgaag cacttccgct ggaaggcctc gtcaccgacc gcgaagacct
     5041 cgtcgaggag gaggatgtct gcatcgagga aggctgcgat ggcgaacccg agccgcatgt
     5101 acatccccga cgagtacgtg cgcaccggcc ggtcgatcgc gtgctcgagc tcggcgaacg
     5161 agacgatctc gtcgaagcgc tcacggatct ccgcccgacg caggccctgg atggagccgt
     5221 tgaggtagac gttctcgcgc cctgtgaagt cggggtggaa acccgccccg agctcgagga
     5281 gcgatcccac gcgcccaccg acggcgatcc gccccttcgt cggccggatg atctcggcga
     5341 taacacgcag gagcgtcgtc ttgcccgacc cgtttcggcc gatcagcccg acggcctcac
     5401 ccggcgcgat gtcgagggag acgtcgcgga gggcccagac ctcctccccg ccggtgcgtc
     5461 cccgcgccac gaacagctcc ttgagcgtcc gcgtgtcccg tgcgcgcacg acgaagcgcc
     5521 gcgagactcc gtcgaccgag atcgccccgc gtctcaatcg ctcacagatc ctttcccatc
     5581 gtcaccgcaa tctcgtcgaa tccgcgcgac cggtaaaggc gtcgcgcgac gtcgctgccg
     5641 ccgaacacgt tcagctcgaa acgatcgagt cctcgcgccc tcgcctcgtc ctcgaacagc
     5701 tccatcgcgc gccggccgag gccccggccg cgcagctcct cgtcgactcg gatctcgtac
     5761 agccatccga cccgacgccc gtcccgactc cgctctgcga agaacagact gccgatcacc
     5821 tcgccgtccg cctcgatcgc gtagagggct gcgcccggcg tcgcgagacc ctccggcagc
     5881 aggttcgcga agtcctcctc ggccttgcgc tcggcgtcgg cgggactcag acgcgcgttt
     5941 cgcaccatgt cgtccgcgta ccccgcctgc gagtccgcga gccagcccga cagctcgtcg
     6001 tcgcccaacg cgcgaagtcg aacgctcatc aggcagccgc cggcgcctcg gtgcgctcac
     6061 gcacgagctc caccagccgc gtcaggctct ccggattcgt cgccggcagg accccgtgcc
     6121 cgagattgaa gatgtgcccc gtgccgccgg cccggtcgag cacctcgagg gcctcgcgct
     6181 cgacgacgtc ccagtcggcg agcagcaccg cgggatcgag gtttccctgc accggcccgc
     6241 cgcctaccag cgcgcgccct tcgtccaggg ggatacgcca gtccaggccg atcacgtcgc
     6301 cgcccgccgc cgccatggcc ggcaggagcg tcgccgtccc cgtgccgaag tggatagtcg
     6361 gcacgtccac cgcgcggagc accgccgagg accagggagc cacgtactgc cggtagtgat
     6421 cgggagagag ggcaccggcc cacgagtcga aaagctggat ggcgtcggcg cccgccccgg
     6481 cctttgcctc tacataggcg gcgaacacgt cgctcagacg ttccagcaac gccccccaca
     6541 gctcgggctc accgagcatg agtgccttgg tgcgcgagag ctcgcggctc ggccttccct
     6601 cgaccagata gcccgcaacg gtgaagggcc cgccgcagaa gccgagcacg gctttctcct
     6661 cgggcagctc cgcccgcacc agccggatcg cctcgagcac gggcgcgaac gcctcctccg
     6721 gctcgggcac gcgtaggcgc gcaacgtcgg ccgcgctgcg aatgggcggg tcgaccacgg
     6781 ggccgacacc ctccacgagc tcgacgttca ctcccatgcc gagcaccggg gtcatgatgt
     6841 ccgcgaagag caccgccgcg tcgacgtcgt ggcggcgtac gggctgcaga gtcacctccg
     6901 cgcacagctc aggcgtgtgc gccacctcga agaaggagtg acgctcgcgg atcgcgcggt
     6961 actcggaaag cgaccggccc gcctggcgca tgaaccagat gggcgtccgc tcgaccggct
     7021 caccccgcgc cgcgcgtacg agaagcgacg cagccacaga gctagactag cccgcgccgt
     7081 ggagcccgtc ggtgagcatc ccgtccccgc aggcccactc gctcctcgct ggttggccca
     7141 cgacgtgggc agcccgcggg cgggagtgct gggcagcgcg cggctccggc tggagaacgc
     7201 gggcaccgcg acgtggcgtt cgcgggggaa actcggcatg cagctcgcgt atcactggct
     7261 cgatccactc ggcaacccga tcgtgtggga cgggatcaga accgcgctgc cgcgcccgat
     7321 cgagccggga gagacgatcg agctcgacgc acctctgctc gcgccgcgac cccccggttc
     7381 ctacgtgctc gccttcgact tcctcgagga gggccatttc tggttcgaag agctcggcat
     7441 ccggagactc gagctgccga tcgcagtggc gccgcgaatc tccgaacggc gcctcgcggt
     7501 tgtcgtgcac ggaggcgtcg acccggagac cgacgccgcg ctcgaggctc aggaggagag
     7561 cctggtcgac gagggcgccg tcgccacagc gcatctcgta ggcggcgccg tccccgcccc
     7621 ggactggtca cgccgtgtgc tcgacctgca cgcggagggc tacgcggcgg tggggggcgc
     7681 cgtcgagccg gaggagcgtc gactccgcaa gcgcttgaaa ccgtgggcgc cgggcggcgg
     7741 gcgaagcccc ggcttcggcc atccgctcct gcttccctcg ctcctcgccg gcgaggagcc
     7801 cggagagcac gagggcctgc ccgccttcga aggagaggac gccctgttcg atgggcgaat
     7861 cgtgatcagg cttcggcggc gatccggtcg tcgacctgcc tgaagacgag cgcgcccagc
     7921 agcatgaaga cgacggtcgc cacgcagagg tagacgaaat cggccgtcgg gggcgcgtcg
     7981 ccgtagaaga gcggcgtgcg gagagattcg atcgccggcg tgaggggatt gccccagtgc
     8041 aggagatcga cgagccacgg gtgcgccgcc gcgccgggca gatcctcgag ccggtagagg
     8101 atcggcgtga ggaagaacca ggggagaagc agcgccgcga cgatgtgctc gacgtcacgg
     8161 aaggcggcgt tcgcagaggc gaccatgagc gagaacccgc cgacgaaggc gacaatcacg
     8221 accgcgaccg gaaccgccat ccacacaccg ccccgtgcat cggcctgaaa ggcgagcgac
     8281 agcacgatca cgatcgccag catcacgacg aacgagacga gctgggtggc aaccgtcgac
     8341 agcggtacga gctgacgcgg gaagcggacc ttgcggatca ggttgacgtt ctcgagcagg
     8401 ctgcgcgacg agctctgcag cgacgtggcg aagaagaccc aggccggcag gccgcagagc
     8461 aggaacagcc agtagttgtc gaagtcggtc gccgtcgcct tccagagcac cgagaagacg
     8521 accaggtaca ccgccatcag catcagcggg tgcgcaagcg accaggcgag cccgaggacc
     8581 gaccccttgt acttcgcccg caggtcgcgc cggaagaggc tgccgaagag ctcgcggtag
     8641 cggaagagat cggcgtagac gctcattgtg cgcttcatgt aagcaagagc accaggcgac
     8701 cgggcgctgc tctacgcttt cggcgtggaa gcggccatcg cgatcgccgc cttgcccgtt
     8761 tcggcgctcg ttctgtgggc gctcctccgg acgtccgcgg ggcgccggct cgtggcggtg
     8821 ccaaccggag agcgctggca cgcgcgtgcc actccgacct tcggtggcct ggggatctac
     8881 gcggggctgg ctgcaggaat cggcctggcc gtcgccaccg gcgcggtcga ggcgagctcg
     8941 gagctagcgg gcgtcgcggc cggcgcgtcg ctgctcttcg tcgcgggggt cgtcgacgat
     9001 gtccgccacc tgagcccgat cgtcaagctc ggcgcccagt tcgcggcggc cgggatcgcg
     9061 atcgcaagcg ggctcaccgt ggagctcgtc gggaacgaca ttctggcgac gatcatcgcg
     9121 ctcgtctggc tggtgggcat cacgaacgcc ttcaacctgc tcgacaacat ggacggtctc
     9181 gccgccaccc tcgcgacgat cgcctgcggc tacttcgcta tcgcggccgc cacggacgac
     9241 cccgtcaacc agctcgtgct cgtaatcgcg ctcgctctgg gcgccgcgtg cctcggcttt
     9301 cttccgttca atcttcgacc gggcggtgcc gcggccgtct tcatgggcga ctcggggagc
     9361 caggtgatcg gcttcacgct cgcctcgctc gcgctcgcct cgagctggac cgtcgccgga
     9421 acgaccgcgg cgaccgtact gctgccgctg ctcgtgctgg cgattccgat cctcgacacg
     9481 tcgctcgtca cgatcgtgcg gctgatcgag cgacgacccg taacccaagg cggcacggac
     9541 cacacctcac accgcctcgt ctactacggc ctctccgagg ggaaggcggt cgggctcctc
     9601 gccctgatcg cgatcgccct gggcgcgacc gcggtcgcgt acaacatcct cgacaacgca
     9661 cggatcacgg cgatcggtgt cctcgtcacc ttcgcgatcc tggtgcagtt tgcgtcgttc
     9721 ctctccgacc tcgaggaacg ccagcgaagc ggcgagaccg gcgaagagcc gtccctgctg
     9781 caggctctct tctctcagcc tcgccggctg ctcgagatcc tgctcgactt cgtgctcatc
     9841 tgcgcgtcgt tcctcgcctc ctacctgctg attgtgggcg gcgaaggggg cgaatggcaa
     9901 cgcgggacgt tcctcgctgc gctgcccatc ctgctcggtc tccgcttcct cgcctttacc
     9961 gcgctcggcg tttaccgtcg cgtgtggcgg ttcgccggcc ccgtcgacct cgcaatgatc
    10021 gcactcggct gcgtgctctc gggtgcggcg gcgctcggcg tgctcgtggc gatccgcccc
    10081 ctcgacgact tcccctggca ggtcttcgta ctggatgcgg tcctctgctc ggtggtcgtc
    10141 gccggctcgc gtctgatctt ccgagccgct gcgctcttcc atgcgcggcg cagcgtggaa
    10201 acgcgcgcga gggtgctcgt ggtcggtgcg gggcgcttcg ggcgaagcgt cgcacgcgag
    10261 ctccaggaga cgccgggaac gcgggtcgtc ggcttcctcg acgacaatcc ggccgttcgc
    10321 cgccgccgcg tccgcggcgt gatggtgatc ggcggcacgc acgagatcga gcgggcgctc
    10381 gagcgagcgc atcccgacga ggtgctcgtc acgatccccg acgcgccggg cgaccggctc
    10441 gatcgcatcg tcgaggcgag tcgcgcggca ggggtaccct gtcggctcgt gcgccgccag
    10501 acggaagtcc ccccgacgct ggtcgagacg aacgtttcgt gaccgtctcc gcactgtccg
    10561 agcgcgtagg gcgcaggact gcggtcgtgt cgctcggcgt cgcctacctc gccttcgcga
    10621 tcctctacgg gtggcaggcc tggcgccgcg tgacaccaac ggtcttcacc gacgagctcg
    10681 agttcgcgca ggtgtcgcgg gcgatctcgg agacgggcag cccggcgcga cggggcgagg
    10741 ggttcgaggc tggcctgtac gagtacatcg tcgcccctgc gtggtggctc gactcggtct
    10801 cggacagcta cggggtgatc aagctggtgg gggtggccgc gatgacggct gcgatcttcc
    10861 ccgcctacgg gctcgcgcgg ctcgccgtct cgcgcccgct ggcgctcttc gcggccgtgc
    10921 tcaccgtcgc cgccccggcg ctcgtgtatg cgccgtttct cgtcggggag ccgctcgctt
    10981 atccggcgtt cacgctcggc ctgttcctga tcgccaggtg gatcgcgcgt cccagctggt
    11041 atcgcttcgg cattgcactc gtgggctgtg cgttcggcat ggccgtgcga acgcagctcg
    11101 ccgttctctt cgcggcgctc ttcctcgctc ttgtcgcggt cgcctggcgc accgagcgga
    11161 tggagcgcta ccgcgccacg tggacgcgct gggactgggc cggagtggtg acgatcgcgg
    11221 tcggcggcgt gctggcgttc tcggccttca tgggtcatcg ctccgagagc tggtacgtat
    11281 cgacgggctt cgagaagcag aagatgtacg agttcggcat ctgggccacg ggagccctcg
    11341 ggatcggcat cggcctgctg ccactgatcg ccgggctggc cgccctcgtc acgccgaagg
    11401 gtgagacact gacagacgag cgtcgcgctt tcgtcaccgc ggccgtcgcg gccatcgcag
    11461 ctgtcgcctt ctactcggct gtcaaggcga cgttcgtgtc gatcgtctgg tcgacgatcg
    11521 tcgcggagcg caacatgatc tacctcgtgc ccgtgctggc cggcggcacc gcgctatttc
    11581 tcgagcgccg ggccgcgcgc tggtgggcag tcgtcgcggc cggcctcttc gcggtctacc
    11641 tggtgcgaac gacaccgctg aagatcgacc tctaccccta ctacgaggca cacgggctcg
    11701 cgatcgccgc cttcgcaaac cgaatcttca tctggccggc ggcgacgatc gagaacgctc
    11761 tgaccatcgt cgccggcgtg gcgaccctcc tgctcctcgc cgtcggagca cagcactggc
    11821 ggagagccgc gactgtgctc ggcaccgtcg ccgttgcgtt cacgatcggc tggtccctca
    11881 ccaccgagat ctacggggcg gagagcgagc gtgacctctc gaaccggatt gcgcagaacc
    11941 tcccgacgcc cgcgaactgg ctcgacgagg cgacgggtgg ggagtcaacc gtgtttctcg
    12001 gccagcacct ctccgatccg accggcgtct ggctgctcga gttctggaac cgctcggtcg
    12061 agaaggtctg gagcaccgac ggatcagcgc ccggcccggg gccgattctc acaccggacc
    12121 tggcgatcac cgacgggacg ctgactccga gcccggagac cgagtgggcg gtgaccgcga
    12181 acggagtcac cctgcgggct gaccagcgcc tggccctggg gacgacctac ctctacaagc
    12241 tcgacggccc gctacaggtc aagttcgcgc aggacggcgt ctactccgac ggctggatgg
    12301 gacggaaggc ctcctactcg cgctacgacg gcggcgatct cgggccgaca ctggccaagg
    12361 tgagtgtctc gcgcaccgcc tggtgcaccg acaccgacgt gcccggcaac gtcgtcgttc
    12421 agatcggcac cgccgccgtg gtcgacaagc agcccgcgat cggccgagtc acggacgagg
    12481 agcggttcgt catcaaagcc tgcgaggtga agcccgtcgt cttccggccg ccgcccgggc
    12541 cgtggcgcat cgaggtccgg atagggccga ccttcgtgcc cgccgagctc gactccagcc
    12601 tcaacgaccg gcgcgagctc ggagccgtcg tcgcgttcga cgtcgttccg atcgacgctg
    12661 cgacgaggta gctgaaccga aagcggagga gcaccctggc caccgggccg ctgcgctcga
    12721 ggccccgtag cagggcggcg agcagccgcg gcagggccac gttctgcggc acgaagaaag
    12781 gccgcaccac gacgtcgacg aatccggccg cgtacagatc gtcgagcacc tcctcgaccg
    12841 ggtactggcg cgggttcaga tcgaacacga gcttcacggc cgtatacgag cggacgcgcc
    12901 ggaagaagct gcgccggtcg cgagcgtagt agagcgcgcg aaacaacgtc gtcgcggcca
    12961 cgggccgcgg cggagcgtag tcgttgaggt cgccgagctc gagcggcact gcgtcaccga
    13021 ggcgagtgcg cgccgctgcg accatctccg gcgttgcgtc gacgccacgg tagctcaagc
    13081 cccgcgccat cagccgctcg ccgagccccc cgtcgccgca ggcaaggtcg agaacctcag
    13141 agcgagcttc gagccggcgc ccgagggcga tgacgagcgc cgctcgatgc tcgaggtacg
    13201 catccgcatc cgcgtactgc tggtcggtcc agccggcagc cttccgtgag tactgatcgg
    13261 ccttcgtcac agcgcaccca ggcgctcggg gtgcttgcgg atgaagcggg ccatgcccct
    13321 tgcgtgccag agagtatggc gactgaggaa gcgatggtcg atgacggcgg cgtacgcgtg
    13381 cctgaccacc gcttcgggca cgtaccagcg ctcccaaccc gcttgcgccg ctcggtagca
    13441 gaggtcgatg tcctcgacgt agtgacggta gccggcgtcc cagccgccga tctcctcgag
    13501 catctcgcgt cgctgtagca ggaaggcacc gagcagccaa tcggcccgga cggggcgctc
    13561 gggtcgctcg tcaaggtggt agtgctcgac ctgccgctgg taggggggcc tgagcagacg
    13621 caacggcgtg cggcgctcca atgtgccccg aacggtcggg aagcgccggc gcgacggctg
    13681 ccacgtgccg tccggccaga gaagttgcgg gccggcgatc ccgcagcgag gatgctcgtc
    13741 cgcgaaccgg ttcaggacgg cgaccgcgcc gacttcgggc acggcgtcgg gattggcgac
    13801 gagcacgaac tcgccgccgg tagcagcgac accctggttg acgttcgcgg cgaacgtgcg
    13861 cggccgggcg ttcgccaaca cccgcgtccc cggcgcaacc gtcccgaccg agccctcgac
    13921 gttggcgatc acgacgagct cgtccacctg aggagcgagc gccggcaaca gccgagtgag
    13981 ctccgcagcg tgcccgtgcg agacgacgac cgcggacacg ctcacgcgcc gagcacctcc
    14041 cgataggcgg caaccgtgcg ctccgccgtc gattgccagc tgaacgcgcg agcccgctcc
    14101 agcccggcgg cgacgaggct ctcccgctcg gccatcgctc gaccgatccc ctcgccgagc
    14161 gccgcctcgg ccacgacgac ggcggcatcg ccggcgactt cgagcagcgc cggctccgga
    14221 acggtgacga cgggacagcc gcaggccatg gcctcgagca ccggcaggcc gaatccctcg
    14281 tagcgcgagg cctgcacgag gctgacagcc tcccggtaga gggccgccag gcgctcgtcg
    14341 tccacgtagc cgagcaagcg ggcgccgcca cggcgcagct gtgcggcaag ggcactgtcc
    14401 tt
//
LOCUS       DBNGNK010000391         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1712922,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000391 DBNGNK010000000
VERSION     DBNGNK010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1712922"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="AC150V_11635"
     CDS             complement(<1..878)
                     /locus_tag="AC150V_11635"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR3763613.1"
                     /translation="MLTLPSGQDAFEEIEEYLRSHGFFRPGAEDLSADLYLGYGLSDP
                     LRRAPAAAPAEPCPIPLAACAVRPAGYVAHNGNRETSGARRTSEALELGAWEPTWPAD
                     DYAAAIEAVRAAIRRGDVYQVNLVQHLVAGFDGTPSALASRLARLNPAPPRTFSGDGW
                     GVVSASPERFLARRGRRVWTEPIKGTRPLGRALELQSSAKDAAEHVMIVDLERNDLSR
                     VCEAGSVRWPELMAVRSLAGVEHMVSTVEGTLRAGVGLAELLAATFPGGSVTGAPKIA
                     AIEHIAALEAVGRGAS"
     gene            complement(881..2182)
                     /locus_tag="AC150V_11640"
     CDS             complement(881..2182)
                     /locus_tag="AC150V_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR3763614.1"
                     /translation="MTLREEATELLRALLRVNTANPPGNESRAVEVLRDYFALAGIPT
                     TLPVRDPGRASIVACLPGGAGPSLVLLSHTDTVTADPTEWERDPWSGDLVDGEIWGRG
                     ALDMKGQVAAAAVAFASLWREGFRPSGDVLFVSTADEEVGVPEGQVSPGLQWLAGEHP
                     ELVRGDLALNEGGGDRVVLGERVYYLCSVAEKMSAPFVLRVHGRSGHASMPGIADNAL
                     VKASRYVEALDRLVPDRVLIPEVQRLLELVLGEEPPLGEALERARALHPLAGDLVEPL
                     LSLTLSPTMIEASRQRNVIPAVCEVTVDCRLLPGMTPEEVEPAIRAALGNGRYELEWI
                     ERWGGTRSPVDSPLWGALADFVASIEPGAQLAPICSPGFTDSHWLREQFDTVAYGFFP
                     LRAMDPQVAARLVHSADERIAVDDLELAVDAYRYVATHLGE"
     gene            complement(2182..>2433)
                     /locus_tag="AC150V_11645"
     CDS             complement(2182..>2433)
                     /locus_tag="AC150V_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HIR3763615.1"
                     /translation="AELYVHGRDHEELYQLAESLIELDEWVQAWRVRHYRVVARIIGD
                     EVIGTQGTPVEVLGRLIHRVFYPELWAVRNELTALSKRT"
BASE COUNT          403 a          875 c          831 g          324 t
ORIGIN      
        1 atcgaagcgc cgcgtccgac ggcctcgagc gccgcgatgt gctcgatcgc agcgatcttg
       61 ggagcgcctg tcaccgaacc gcccggaaag gtcgcggcaa gaagctcggc aagcccgacg
      121 ccagcacgaa gggtgccctc caccgtcgag accatatgct cgacacccgc gagcgagcgc
      181 acggccatca gctcgggcca gcgaacgcta cccgcctcgc agacgcgcga gaggtcgttg
      241 cgctcgagat cgacgatcat gacgtgctcg gcggcgtcct tcgccgacga ctgcagctcc
      301 aacgcccgcc cgagcggccg tgtccccttg atcggctccg tccacacccg gcgcccgcgc
      361 cgcgcgagga agcgctccgg agaggctgac acgacacccc aaccgtcacc ggaaaacgtc
      421 cgtgggggtg ccggattgag acgggccagc cgggaggcga gcgcggacgg cgttccatcg
      481 aatccggcca cgagatgctg tacgaggttg acctggtaga cgtcgccgcg ccggatcgcg
      541 gcgcggacag cttcgatcgc cgcggcgtag tcgtccgcgg gccaggtcgg ctcccacgct
      601 ccgagctcca gggcctccga cgtccgccgc gcaccgctcg tctctctgtt gccattatgg
      661 gcaacatacc cggccggacg gacggcacag gccgcgagcg ggatcgggca gggctccgcc
      721 ggcgccgccg ccggtgctcg ccgcagcggg tcggagagcc cgtagcccag atagagatcg
      781 gcggagagat cctccgcacc gggcctgaag aagccatgcg accgcaggta ctcctcgatc
      841 tcttcgaagg cgtcctggcc cgacgggagc gtgagcatcg tcactcgccc agatgcgtcg
      901 ccacgtagcg gtaggcgtcg acggcgagct cgaggtcgtc gacggcgatc cgttcgtctg
      961 cggagtggac gagccgcgca gcgacctgcg gatccatcgc acgcaacggg aagaacccgt
     1021 atgcgacggt gtcgaactgc tctcgcagcc aatggctgtc ggtgaagccc gggctgcaga
     1081 tcggagcgag ttgcgcacca ggctcgatgc tggcgacgaa gtccgcgagc gccccccaga
     1141 gcggcgagtc gacaggtgac cgcgtcccgc cccagcgctc gatccactcg agctcgtagc
     1201 gtccgttgcc gagtgccgct ctgatcgccg gctcgacttc ctccggcgtc atccccggca
     1261 agaggcgaca gtcgaccgtc acctcgcaca cggccgggat gacgttccgc tgacgcgacg
     1321 cctcgatcat ggtcggggac agggtcagcg acagcagcgg ctcgacgagg tcgcccgcca
     1381 gcggatgaag cgcgcgcgcc cgctcaagtg cctcgccgag cggcggctcc tcccccagga
     1441 cgagctcgag caggcgttgc acctccggga tgagaacgcg atccgggaca aggcgatcga
     1501 gcgcctccac gtagcgagag gccttgacga gcgcgttgtc ggcgatgccg ggcatcgacg
     1561 cgtggccgct cctgccgtgc acgcgcagga cgaacggcgc gctcatcttc tcggcaaccg
     1621 agcagaggta gtagacacgc tccccgagaa cgacgcggtc gccaccgcct tcgttcagag
     1681 cgaggtcgcc acggacgagc tcggggtgct cccccgcgag ccactgcagg ccgggtgaga
     1741 cctgaccctc gggcacgccg acctcctcgt cggcggtgga gacgaagaga acgtcgccgg
     1801 acggccggaa gccctcccgc cagagcgacg cgaacgcgac cgcggcggcg gccacctgcc
     1861 ccttcatgtc cagcgcgccg cgaccccaga tctccccgtc gacgaggtcg cccgaccacg
     1921 gatcgcgttc ccactcggtc ggatcggccg tcaccgtgtc ggtatggctg agcagaacga
     1981 gggacggccc cgcgccgccc ggaagacacg cgacgatgct cgcccgtccg gggtcgcgaa
     2041 ccggaagcgt ggtcgggatg cccgcgaggg cgaagtagtc ccgcagcacc tctacggcac
     2101 gcgactcgtt cccgggcgga ttcgccgtat tcacccgcag gagcgcccgc aggagctcgg
     2161 tcgcctcctc tcggagggtc atcacgtgcg cttcgaaagg gcggtcagct cgttgcggac
     2221 ggcccagagc tccgggtaga agacgcggtg gatgagccgg ccgagcacct cgaccggcgt
     2281 cccctgcgtc ccgatcacct cgtcgccgat tatgcgggcg acgactcggt agtggcgcac
     2341 gcgccaggcc tgaacccact cgtcgagctc gatcagcgac tcggcgagct ggtagagctc
     2401 ctcgtggtcg cgaccgtgga cgtacagctc cgc
//
LOCUS       DBNGNK010000392         2391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_928378,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000392 DBNGNK010000000
VERSION     DBNGNK010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_928378"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1881)
                     /locus_tag="AC150V_11650"
     CDS             complement(<1..1881)
                     /locus_tag="AC150V_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR3763616.1"
                     /translation="MASIDLAEVPVHEANELLRRHGEQGDDVELLNPDARHHLGVGLT
                     HPINVRIRGSAGYFCAGLTDGPRFEVDGNVGWGLADNMLGGSVVVRGNAGAIAGVAVR
                     GAEVVVHGNIGSRAAQVMKEGTLCCAGNASFMAGYLMYGGTLIICGDSGEQVGQDMAG
                     GTIFVGGEIVSLGTDAEVADITAEDEESVRSFLERYGIGFAGGFKKIVNAGKNLHYPR
                     TEPRVRVVPFFVGSERTDYWNDKVQEDIAVKAQIGRYRIRGYGAARPLPHLGDISFRR
                     DLAAVDSDPDVVEKVELATSLGGRFGAKPIELSMPVMIAPMSYGALSKSTKIALAKAS
                     ARSDICDNTGEGGLIPEVREAAKRVVVQCLGGRLGWSVHAMRQADGIEIYISQGAKPG
                     LGGQLMAKKVTEDIARLRGIPAGIDLRSPSRHPDVLGADDLVIKVEEFREATGWEKPV
                     SVKLGAGRVRDDIKIAYKDGFDYVCLDGVQGSTGAAGNEVLEFVGIPTLSAIMEALDA
                     LEEIEASGMPLVLMGGIKDGIDTAKAIALGAHAVALGTSAIIAGGCIACLQCAVGQCV
                     VGIATQDPVYEARYDIEAESWNIHRYLESVRWQLASVVRAHGYSSVYDLSRTDLVALT
                     PEA"
     gene            complement(1863..>2391)
                     /locus_tag="AC150V_11655"
     CDS             complement(1863..>2391)
                     /locus_tag="AC150V_11655"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR3763617.1"
                     /translation="AHDVDDVYHVAGFTGTHGIGHVRLATESDVTPDRSHPFWATGFA
                     DVAIVHNGQITNYWKMRRRLERRGMEFHTDNDSELIAVYLAEKLEQGVSLSDALEQAI
                     GDLDGTFSFLVSTKDEIGYAKDRLAAKPMVMIESDDLVAIASEEVSLNKLFPGSSLQT
                     NEPAPGTFCTWRRST"
BASE COUNT          363 a          826 c          796 g          406 t
ORIGIN      
        1 cgcctcgggc gtcagggcga cgaggtccgt gcgcgagagg tcgtagaccg aggagtagcc
       61 gtgcgcccgc acgaccgacg ccagctgcca gcggacggat tccaggtagc ggtggatgtt
      121 ccacgactcg gcctcgatgt cgtagcgggc ctcgtagacg gggtcctggg tcgcgatgcc
      181 gacgacgcac tggccgacgg cgcactgcag gcaggcgatg cagccgccgg caatgatcgc
      241 gctcgttccg agagcgactg cgtgggcgcc gagggcgatc gccttcgcag tgtcgatccc
      301 gtccttgatc ccgcccatga gcacgagcgg catcccgctc gcttcgatct cctcgagcgc
      361 gtccagcgcc tccatgatcg cggacagcgt cgggatgccg acgaactcca gcacctcgtt
      421 gccggcggca ccggtcgacc cctgcacccc gtcgaggcag acatagtcga agccgtcctt
      481 gtacgcgatc ttgatgtcgt cacggacgcg accggccccg agcttgacgg agacaggctt
      541 ctcccagccg gtcgcctcgc ggaactcctc gactttgatc accagatcgt ccgcgccgag
      601 cacgtcgggg tgtcgcgaag gcgagcgcag gtcgatgccc gcggggatgc cacgaaggcg
      661 agcgatgtct tcggtcacct tcttcgccat cagctggcct cccaggcccg gtttggcgcc
      721 ttgcgagatg tagatctcga tcccgtcggc ctggcgcatc gcgtgcacgc tccagccgag
      781 ccgcccgccg aggcactgca cgacgactcg cttcgccgcc tcccgcacct cggggatcag
      841 gccaccctca ccggtgttgt cgcagatgtc cgagcgcgcc gatgccttcg cgagcgcgat
      901 cttggtcgac ttggaaagcg ccccgtagct catcggggcg atcatcaccg gcatcgagag
      961 ctcgatcggc ttggcgccga agcggccgcc gagcgacgtc gcgagctcga ccttctcgac
     1021 gacgtccggg tcggagtcga cggcggccag gtcgcggcgg aacgagatat cgcctagatg
     1081 cggcagcggc cgggcagcgc cgtagcctcg gattcggtag cggccgatct gcgccttcac
     1141 ggcgatgtcc tcctgcacct tgtcgttcca gtagtcggtg cgctccgagc cgacgaagaa
     1201 cggcaccacc cgcactcgcg gctcagtgcg cgggtagtgg agattcttgc ccgcgttgac
     1261 gatcttcttg aagccgccgg cgaagccgat tccgtagcgc tccaggaacg agcgcacgct
     1321 ctcctcgtcc tccgcggtga tgtcggccac ctcggcgtcg gtgccgagcg agacgatctc
     1381 gccgccgacg aagatcgtgc cgcccgccat gtcctgtccg acctgctcac ccgagtcgcc
     1441 gcagatgatc agtgtgcccc cgtacatgag gtagccggcc atgaagctgg cgttgccggc
     1501 gcagcagagc gtcccctcct tcatcacctg ggccgctcgc gagccgatgt tcccgtgcac
     1561 gacaacctcg gccccgcgca ccgcgactcc ggcgatggcg cctgcgtttc cgcgcaccac
     1621 gacgctgccg ccgagcatgt tgtcggccag gccccagccg acgttgccgt cgacctcgaa
     1681 gcgcgggccg tcggtcaggc ccgcgcagaa atacccggcg gagccacgga tccgcacgtt
     1741 gatcggatgg gtcagcccga cgcctagatg atggcgggcg tcggggttca gcagctcgac
     1801 gtcgtcgccc tgctccccgt ggcggcgaag cagctcgttc gcctcgtgga ccggaacctc
     1861 ggctaggtcg atcgacgcca cgtgcagaag gttcccggcg ccggctcgtt ggtctggagc
     1921 gagctgcccg ggaagagctt attgagtgac acctcttcgg atgcgatcgc gacgagatcg
     1981 tcgctctcga tcatcaccat cggcttggcg gcgaggcggt ccttcgcgta gccgatctcg
     2041 tccttcgtcg agacgagaaa ggagaacgtg ccgtcgaggt cgccgatcgc ctgctcgagc
     2101 gcgtccgaga gcgatacgcc ctgctcgagc ttctcggcca gatagacggc gatcagttcc
     2161 gagtcgttgt cggtgtggaa ctccatgccg cggcgttcga ggcgacgccg catcttccag
     2221 tagttagtga tctggccgtt gtggacgatg gcgacgtccg cgaagcctgt cgcccagaac
     2281 gggtgcgacc ggtcgggcgt cacgtcggac tcggttgcca ggcgaacgtg gccgatcccg
     2341 tgcgtgcctg tgaagcccgc gacgtggtac acgtcgtcga cgtcgtgcgc g
//
LOCUS       DBNGNK010000393         2829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1500544,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000393 DBNGNK010000000
VERSION     DBNGNK010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1500544"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="AC150V_11660"
     CDS             complement(<1..526)
                     /locus_tag="AC150V_11660"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR3763618.1"
                     /translation="MPQRLAVNDRGRDPIPRSLRVAAGVSWRLLLLVALLIVVGLAVS
                     RLRLVLVPAFVAVLGAAFLGPPTRRLRAFGLPPALAALATMLAAMLVVAGIIAALSPQ
                     VASDIGETEISLTSVRDDARTWLVKSPFGLSGSQVDRLFDRVERELRKKESVLVTGAF
                     SGALVAVEVFAGVLL"
     gene            complement(776..1285)
                     /locus_tag="AC150V_11665"
     CDS             complement(776..1285)
                     /locus_tag="AC150V_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017733728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR3763619.1"
                     /translation="MEELNSLEDVFLEQLADLRSAEQQLVAALPKVAAAASSDELRSA
                     FEEHLEQTRGHVGRLEKIIADLPATAPGEHCEGMEGLIREGEQLLAKPGDAGSKDAAL
                     IAAAQRIEHYEIAAYGTARTLADELGYEDAKDLLAKTLDEEADADERLTRIATGGLLS
                     AGVNRAATG"
     gene            complement(1373..2329)
                     /locus_tag="AC150V_11670"
     CDS             complement(1373..2329)
                     /locus_tag="AC150V_11670"
                     /inference="COORDINATES: protein motif:HMM:NF012573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HIR3763620.1"
                     /translation="MRVKSQWLIFVGVLVGIIVAAAPSTAAKQQQRCTERKTGTGQAD
                     ILPGTPGGDRIVGGAGNDQISGQLGNDCLDGGPGADQLNGGAGDDELNGRDGADDLSG
                     GPGADRLDGGGGFDFLSGGLGADRVMGMAGPDVLSGGEGRDSLHGGLGDDFLSGGLAG
                     DELFGDAGNDLLTGGQGGDRMDGGSGSDEMNGGPGPDIMFGNTGDDDMTGGSSNDQVN
                     GGAGDDEITGGAGADRLVGGGGVDYLTGGLGNDLLSGGAGADRLDGGPGVNQYNAGPG
                     DDLVNSVNGVPETVNCGTGNDLVYADATDTVRSCEDVNRIAR"
     gene            2605..>2829
                     /locus_tag="AC150V_11675"
     CDS             2605..>2829
                     /locus_tag="AC150V_11675"
                     /inference="COORDINATES: protein motif:HMM:NF015721.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HIR3763621.1"
                     /translation="MTDSGTTSIADVVVEKIAGLAVSEVGGVHSTGSTRQRALASVTD
                     AVGLGDGDRRGVDVEVGEYETAIDLEIVVEY"
BASE COUNT          445 a          997 c          931 g          456 t
ORIGIN      
        1 caagcagaac cccggcgaag acctcgactg cgacgagcgc accggagaag gcgcccgtga
       61 ccagcacgct ctccttcttg cggagctcgc gttccactcg gtcgaacagg cggtctacct
      121 ggcttccgga cagcccgaac gggcttttaa cgagccacgt ccgggcatcg tcgcgcacgc
      181 tggtgaggct gatctccgtc tctccgatgt cggaggccac ctgaggcgac agcgcggcaa
      241 tgattcccgc gacgacgagc atggcggcga gcatcgtcgc aagagccgcg agggccggcg
      301 gaaggccaaa cgctctcagc cgacgcgtcg gaggcccgag gaaagccgca cccagcacgg
      361 cgacgaaggc cggcacgagg acgagccgca acctcgacac ggcgaggccg acgacgatca
      421 gcagcgcgac gaggagcagc aggcgccaac tcactccagc ggcaacccgc agcgagcgag
      481 ggatgggatc tctgccgcgg tcgttcacgg cgagcctctg aggcatcttc gcggcacctc
      541 gtctgaatac ccggaacggc gcgcttcgaa tcgtcctcgc gctcgcgccg agcgaggctg
      601 gaaggacgcc gtcacggtgc gcaaggggac gcccctgctg cgagaagaga cggacgagcc
      661 cacaagccgc tgagccggcc gacgcgcagt ccgcggccta gccgcggcgt gcggcgcaag
      721 ggccgcggcc ccggtcgttg actcgaagcc gcggcccaca cgtcgcgtcg ttccgtcagc
      781 cggtcgcggc cctgttgaca ccggcgctca gaaggccacc ggtggcgatc ctcgtcagcc
      841 gctcgtctgc gtctgcttcc tcgtcgagcg tcttggccaa gaggtctttg gcgtcttcgt
      901 agccgagctc gtccgcgagg gttcgcgctg tcccgtaggc agcgatttcg tagtgctcga
      961 tccgctgcgc ggcggcaatg agtgcggcat ccttcgatcc cgcgtcaccg ggcttggcga
     1021 ggagttgctc gccctcccgg atcagcccct ccattccttc acagtgctcg ccgggagctg
     1081 tcgcagggag atccgcgatg atcttctcca gccggcccac gtgaccgcgc gtctgctcga
     1141 ggtgctcctc gaacgcactt cgcagctcat ctgagctcgc cgcggcggcc accttaggga
     1201 gcgccgcgac gagctgctgc tcggccgacc gcaggtcggc caactgctcc aggaagacgt
     1261 cctcgaggga gttcagctct tccatgattc ctccttacgt cgtggtcgat ttcgtcgctc
     1321 gatcgaaccg attccgatgc gagcgaacac gattcgtcgc tcggcccgcc ggctaccggg
     1381 cgatgcggtt gacgtcctcg cagctccgga cggtgtcagt cgcgtccgcg tagacgaggt
     1441 cgttaccggt gccgcaattg acggtctcgg gcacaccgtt caccgagttg acgagatcgt
     1501 cgcctgggcc ggcgttgtac tggttcacac cggggccgcc gtcgaggcga tcggcgcccg
     1561 cgccaccgga gagcaggtcg ttcccgagac ctcccgtgag gtagtcgaca ccgccgccgc
     1621 cgacgagccg gtcggcgccg gcgcctccgg tgatctcgtc gtcaccggca ccgccgttca
     1681 cctgatcgtt gctggagcca ccggtcatgt cgtcatcgcc ggtgttgccg aacatgatgt
     1741 cgggccccgg gccgccgttc atctcgtcgc tgccggagcc gccgtccatc ctgtcgccgc
     1801 cctgaccacc cgtcagcagg tcgttgccgg cgtcgccgaa gagctcgtcg ccggctaagc
     1861 cgcccgacag gaagtcgtcg ccgagaccgc cgtgcaggct gtccctgccc tcgccgccgg
     1921 aaagcacgtc gggaccggcc attcccatga cacgatcggc gccgaggcct ccagagagaa
     1981 agtcgaagcc gccgccaccg tcgagtcggt ccgccccggg gccgccggat aggtcgtccg
     2041 ctccatcacg gccgttcagc tcgtcgtcgc ccgcgccgcc gttcagctga tcagcgccgg
     2101 ggccgccgtc gaggcaatcg ttgccgagct ggcctgagat ctgatcgttg ccggccccgc
     2161 cgacgatccg gtctcccccg ggagtcccgg gcaggatgtc ggcttggccc gtgccggtct
     2221 tcctctctgt gcagcgttgc tgctgcttcg ccgctgtcga cggcgccgcc gcgacgatga
     2281 tgccgacgag gacgcccacg aagatcaacc actgtgactt cacgcgcatg tcgtccacct
     2341 ccgatggatc tcgttgcctg ctgggtggac tacccggccc cctgccgttg aaaacatgag
     2401 ccgtcgttcc cgccggcccc caaaatggat tgcaggtaat cggaacgggc tcgcgagcag
     2461 tcggctcgcc gggtgcgccc gtttagcgag cccggtgtcc gggtacgcag cgtctacaca
     2521 gtcgcacgaa cgtgcccaac tcccagggaa aggaatctca tggcaagcac aagcttggct
     2581 tctagctcca gcgcgaacgc tctcgtgact gacagcggaa cgacgagcat cgcggacgtc
     2641 gtcgtcgaga agatcgcagg tctcgctgta tccgaggtag gcggcgtgca ctccacgggt
     2701 tcgacgcgac agcgggcgct cgcatccgtc accgacgccg tcgggctcgg ggacggcgac
     2761 aggcgcggcg tggacgtcga ggtcggcgag tacgagacgg caatcgatct cgagatcgtg
     2821 gtcgagtac
//
LOCUS       DBNGNK010000394         5304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_570407,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000394 DBNGNK010000000
VERSION     DBNGNK010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5304
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_570407"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="AC150V_11680"
     CDS             complement(<1..517)
                     /locus_tag="AC150V_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3763622.1"
                     /translation="MSLAVEQLELGPIGTNCYIVRAELGAPEAVVVDPSGDAATLRLR
                     IAEMGSSCAAILLTHGHWDHLVGVADLAEGTEAPVYMPAAERVLLDEPAAFTPPGVTV
                     RGYTPDVELEGGETIEAAGIRFDVLSVPGHSPGHVAYRAEGCLFSGDVLFAGSVGRTD
                     LPGGDWDTLLAS"
     gene            568..903
                     /locus_tag="AC150V_11685"
     CDS             568..903
                     /locus_tag="AC150V_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR3763623.1"
                     /translation="MLSWDLTAIETPGGTVSPHVLFSDDSRAVLIRIAPGQALGDHQV
                     RERAFVCVVEGSVTVVCGEDEFEAGVGTLVTFDPGERHSVRSATGARILLLLAPWPAP
                     DHYGPAEAG"
     gene            complement(915..1301)
                     /locus_tag="AC150V_11690"
     CDS             complement(915..1301)
                     /locus_tag="AC150V_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763624.1"
                     /translation="MATRKQRKRRAKGQRHEYVWEDSEGNVIDPAEVRKNGDGRTEKD
                     KPASKPQRGRRTVQPPSWQRSTKRGLIFGPIFFLIVLLLSGGNLSITGALLNAILLLA
                     VFIPFSYVLDKLVYRQFQKRQAKGEQ"
     gene            complement(1333..1611)
                     /locus_tag="AC150V_11695"
     CDS             complement(1333..1611)
                     /locus_tag="AC150V_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763625.1"
                     /translation="MEAAPPKIPKHPYRDSAVFHGALAGLLVVVAWLTGGGIGRALVV
                     GLIYFVVATSWAWWRFRRRIEREEAAAAEEQAVTKTTDQTAGDGIGDR"
     gene            complement(1739..2623)
                     /gene="ftcD"
                     /locus_tag="AC150V_11700"
     CDS             complement(1739..2623)
                     /gene="ftcD"
                     /locus_tag="AC150V_11700"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HIR3763626.1"
                     /translation="MLPLEAVPNVSEGRDRTVVDAIAAAFSSSARLLDVHVDPDHNRS
                     VFTLTGGDESLVDSLVAGVARTKELVDLEQQEGVHPRVGAADVVPLVAVSPDSMERAR
                     TAASAVGERLGAELELPVFLYGEAGGGRRPAFFRRGGPHELQRRIDEGELTPDFGPER
                     LDPGAGAVLVGARRPLIAFNVDLVTDDVEIARAIAAAVREADGGLPTVLALGLELPGS
                     GRVQVSMNLEDWEATPPHRVVRRIGELAAEHGVKLAGSELVGLIPAGAATAAAAASLG
                     LRSLDPDQVLELRLLDES"
     gene            2645..2806
                     /locus_tag="AC150V_11705"
     CDS             2645..2806
                     /locus_tag="AC150V_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763627.1"
                     /translation="MDAGSYDSLASLQCANRGCRLCAAAGFPIASLPVFEGHAGQRAY
                     LFGQAPGVV"
     gene            2822..3280
                     /locus_tag="AC150V_11710"
                     /pseudo
     CDS             2822..3280
                     /locus_tag="AC150V_11710"
                     /inference="COORDINATES: protein motif:HMM:NF015146.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
     gene            3291..4025
                     /locus_tag="AC150V_11715"
     CDS             3291..4025
                     /locus_tag="AC150V_11715"
                     /inference="COORDINATES: protein motif:HMM:NF051612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7847 domain-containing protein"
                     /protein_id="HIR3763628.1"
                     /translation="MTVSNILGRSWDLYTRFFARFFVLALVVFLITGLANAIIAQIFD
                     RGSTAGGLLLSLVATAVILVGTYWVQGALVFAVQDARDGSFDTSNEDLLGKVRPYLGT
                     LIVAGILAGFGVAIGIVLLIVPGLILLTWWALIVPVIVLEQRGVIESFGRSRELVRGH
                     GWTVFAIVVIAAIISGVASGAIQLLLSFLPAFLEAWLGSAIAGAVVAPFMAIAVTLMY
                     FNLSEAESMPEVVATETKDSEPEPTA"
     gene            4072..4950
                     /locus_tag="AC150V_11720"
     CDS             4072..4950
                     /locus_tag="AC150V_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR3763629.1"
                     /translation="MDLDRETEHRIDEPVSPEALRETRLSPADAVAAMRIRLPVRGNR
                     KLRRALERANADEELKAWWHVSNVNAVVRLEINDHSWVHIQIVANIALKLLRQLTKHG
                     VEPGVVADYGLEDDDAEVIVVLAALTHCVGMSIHRRGHEDFSLFLSEPKLRELLDGVY
                     EEPTLTVIVSEVLQAITSHRADGEPLSLEAGILRVADALDMAKGRSRIPFERGRVSMH
                     SLSAAAIDEVKIGDGEEKPVQIEVLMNNSSGLFQVDELLKAKLQGSGLEPHVEVIAHI
                     DTADEKRLVPLYRLES"
     gene            5003..5073
                     /locus_tag="AC150V_11725"
     tRNA            5003..5073
                     /locus_tag="AC150V_11725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5035..5037,aa:Gln,seq:ctg)
BASE COUNT          806 a         1786 c         1826 g          886 t
ORIGIN      
        1 tcgacgcgag cagcgtgtcc cagtcgcccc cgggcaggtc cgttcgcccg acagatccgg
       61 cgaacagcac gtcacccgag aagaggcagc cctcggcgcg gtaggcgacg tggcccgggg
      121 agtgccctgg gacagagaga acgtcgaaac ggatgccggc ggcctctatc gtctcgccgc
      181 cctccagctc gacgtcgggt gtgtagccac gcacggtgac gcccggcggc gtgaacgctg
      241 cgggttcatc gagcagaacg cgttctgcgg ccggcatgta gaccggcgcc tcggttccct
      301 cggctagatc ggcaacgccc acgaggtgat cccagtggcc gtgggtgagc aggatcgcgg
      361 cacagctcga tcccatttcc gcgatgcgga ggcgcagcgt cgcggcgtcg ccgctgggat
      421 cgacgacgac tgcctccggc gcgcccagct cagcccgcac gatgtagcaa ttcgtcccga
      481 tcgggccgag ctcgagttgt tcgactgcga ggctcacggc gtcgagtcta gtggtctctc
      541 cgggaggcaa cgactacggt tcgctgagtg ctgtcgtggg atctgaccgc gatcgagacg
      601 cccggcggga cggtgtcccc gcacgtcctc ttctcggacg actcgcgcgc cgtgctcatc
      661 cgtattgcgc ctggccaggc gctcggcgat caccaggtgc gggagcgtgc attcgtctgc
      721 gtcgtcgaag gatcggtcac cgtcgtgtgc ggggaagacg agttcgaagc cggcgtcggc
      781 acgctggtca cattcgatcc cggtgagcgg cattccgtgc gcagcgccac gggcgcacgc
      841 attctcctcc tgctcgcgcc gtggccggcg ccggaccact acggtccggc cgaggccggc
      901 tagtcagccg gccgttactg ctcgcccttc gcctgccgct tctggaactg gcggtagacg
      961 agcttgtcga gcacgtagct gaaggggatg aagacggcga gcagcaggat cgcgttgagg
     1021 agcgcgcccg tgatcgagag gtttccgccg ctcagcagca agacgatgag gaagaagatc
     1081 ggcccgaaga tgagcccacg cttcgtcgag cgctgccagg acggtggctg aacggtgcgg
     1141 cggccgcgtt gtggcttcga tgccggcttg tccttctcgg tgcggccgtc cccgttcttc
     1201 cgtacctcgg ccgggtcgat cacgttccct tccgagtcct cccagacgta ctcgtgccgc
     1261 tgtcccttcg cacgccgctt tcgctgcttg cgggtcgcca tgtgtcgatt atgcccgacg
     1321 atcgaagccg gctcagcgat cacctattcc gtctccggcc gtctgatcgg tcgtcttggt
     1381 caccgcctgc tcctcggcgg ccgccgcctc ctcgcgctcg atccgccgcc ggaagcgcca
     1441 ccaggcccag ctcgtggcga cgacgaagta gatcagcccg acgacgagcg cccgcccgat
     1501 acccccaccc gtcagccagg cgaccacgac cagcagaccg gcgagcgcac cgtgaaagac
     1561 ggcgctgtct cggtagggat gtttcggaat cttgggcggt gccgcctcca aacggacacg
     1621 tcgtcgcggc tgggtctcat gcttcgcgga cctgcgcttc ggtggctggc gcgccatcag
     1681 gcgcgggcgc gagtgtggga tgcacgaggc gtggacaccg ccgcgagtgt attcgcggtc
     1741 agctctcgtc gagcagcctg agctcgagta cctggtccgg atcgagggag cgcagcccca
     1801 gcgacgcggc cgcggccgca gtcgcggcgc cagccgggat caagccgacc agctcggagc
     1861 cggcgagctt cacgccgtgc tcggcggcga gctcgccgat ccgccggacg acccgatgcg
     1921 ggggagtcgc ctcccagtcc tcgaggttca tgctcacctg aacccggccc gatccgggca
     1981 gctcgagacc gagcgccagt acagtgggca ggcccccgtc cgcctcccgc accgccgccg
     2041 cgatcgctcg ggcgatctcg acgtcgtccg tcaccaggtc gacgttgaag gcgatcagcg
     2101 gacggcgcgc gccgacgagc acggcgccgg cacctggatc gagccgctcc ggcccgaagt
     2161 ccggcgtgag ctcaccctcg tcgattcgcc gctggagctc gtgcgggccg ccccgacgga
     2221 agaacgccgg gcggcgccct ccgccggcct cgccgtacag gaacaccggc aactcgagct
     2281 cggcaccgag gcgctcgccg acggcgctgg cggccgtccg cgcacgctcc atgctgtcgg
     2341 gtgagacggc gacgagcggg acgacgtccg cggcaccgac gcgcgggtgc actccctcct
     2401 gctgctcgag atcgacgagc tccttcgtcc gcgcgacacc ggccacgagc gagtcgacga
     2461 gggactcatc gccgcccgtg agcgtgaaga ccgagcgatt gtgatccggg tcgacgtgga
     2521 catcgagcag gcgggcggat gacgagaacg cggcggcgat ggcgtcgacg accgtgcggt
     2581 cacgtccttc cgacacgttc gggactgcct ccaacgggag cacgacgcgt actctacggc
     2641 gctcgtggac gcgggctcgt acgactcact ggcatcgctg cagtgcgcga accgggggtg
     2701 ccggctctgc gccgccgcgg ggtttcccat cgcgtcgctc cccgtcttcg aggggcatgc
     2761 cgggcagcgc gcctacctat tcggccaggc tcccggggtc gtctaagggg aggagcgacg
     2821 accctggcgg ggccgggcgg ggaagacgct acggcgctgg ctccgcctgg aagaagacgc
     2881 gttctacgcg accttctact gcgcgtcggt cacgcgttgc tacccgggcc gggcggcctc
     2941 gggtcgtggc gaccgcacgc cggaagcggc ggagcaggct ctctgcgcag cgtggcgtga
     3001 gggggagctt cggctgctgc agccggacct gatcgtgacg gtcggtgggc tggccgcgcg
     3061 ccgattcctc ggcgtggagc gactgaccga cgcagtcggc aggagtttcg tgcggtcggc
     3121 tgcggtggtc attccgctcc cccacccctc gggcgcgagc ggctggctca acgacccgga
     3181 gaacaaggcg cggctcggaa aggcgctcac acacgtcagg cgtgagttgg cctggcttgc
     3241 cgagaccaac accgagcagg gcagccggtc actgtcgtag gatcacgttt gtgacggtct
     3301 cgaacatcct tggacgctcg tgggatctct acacgcgctt cttcgcgcgc ttcttcgtac
     3361 tcgcgctcgt cgtgttcctg ataacgggcc tagccaacgc gatcatcgcg cagatcttcg
     3421 accgcggttc gaccgcgggc ggattgctgc tgagcctcgt cgcaaccgcc gtgatcctcg
     3481 tcggcaccta ctgggttcag ggcgcactgg tcttcgcggt gcaggacgcc cgcgacggat
     3541 ccttcgacac ctccaacgag gacctgctcg gaaaggtccg cccctacctc ggaacgctca
     3601 tcgtggctgg gattctcgcc ggcttcggcg tagcgatcgg aatcgtcttg ctgatcgtcc
     3661 cgggactgat cctcctgacg tggtgggcgc tgatcgtgcc ggtgatcgtg ctcgagcaga
     3721 gaggcgtgat cgagtcgttc ggacgctcgc gggagctcgt ccgcggccat ggctggacag
     3781 tgttcgcgat cgtcgtgatc gccgccataa tctcgggcgt cgccagcggt gcgattcagc
     3841 tcctcctcag cttcctgccg gcatttctcg aggcttggct cgggagcgcg atcgccggcg
     3901 ccgtcgtcgc cccgttcatg gccatcgctg tgacgttgat gtacttcaac ctctccgaag
     3961 ccgagtcgat gcccgaggta gtcgcgacgg agacgaagga ttcggagccg gagccgaccg
     4021 cctagaaccg ccgggtcgag gatcggacgc cacccacagt aggcttcagg cgtggatctc
     4081 gaccgcgaga ccgagcatcg catcgacgag cccgtctcgc ccgaagccct ccgcgagacg
     4141 cgactcagtc cggcggacgc cgtcgcggcg atgcggattc ggctacccgt ccgcggcaat
     4201 cgcaagctgc gccgggcgct cgagcgcgcc aacgccgatg aggagctcaa ggcctggtgg
     4261 cacgtctcga acgtcaacgc cgtcgtccga ctcgagatca acgaccactc gtgggtgcac
     4321 atccagatcg tcgcgaacat cgctctgaag ctcctgcgac agctgacgaa gcacggcgtc
     4381 gagccgggcg tcgtcgccga ctacgggctg gaggacgatg acgccgaggt gatcgtcgtc
     4441 ctcgcggcgc tcacacactg tgtcgggatg tcgatccacc gccgcggaca cgaggacttc
     4501 agcctgttcc tctccgagcc caagcttcgt gagctgctgg acggcgtcta cgaggagccg
     4561 acgctgacgg tgatcgtgtc ggaggtgctg caggcgatca ccagccatcg cgcagacggc
     4621 gagcccctgt cgctcgaggc aggcatcctg cgcgtcgccg acgccctcga catggcaaag
     4681 ggccggtcgc gcatcccctt cgagcgtggc cgggtgtcga tgcactccct gtcggccgcg
     4741 gcgatcgacg aggtgaagat cggagacggc gaggagaagc cggtgcagat cgaggtcctg
     4801 atgaacaact cgtccggtct gttccaggtc gacgagctgc tgaaggccaa gctgcagggg
     4861 tcgggtctgg agccgcacgt cgaggtgatc gcccatatcg acaccgccga cgagaagcgg
     4921 ctcgtgcccc tgtaccgact cgagtcgtag acgttcggcg actgtcggcc ctcaggggca
     4981 gacgctagac tcagacgagc cctgcggggt cgtctaatgg taggacgctg gattctggct
     5041 ccagtagtca aggttcgagt ccttgccccg cagctcgaaa gcggcgattt gcaggatctt
     5101 tctaggtagc gaccgcgggt ggcaacggcg atgtggtcac ttgtggtcca ttgtaaagcg
     5161 tgttacttca cattgtaaag cgactcattt tgggacaccc ccacgggttg cgtgtggtgt
     5221 ggcggcgtcc gccgtcgacg cgcggccatg ggggtgccgc taggtggctt ccggtgcgcg
     5281 cttctctgga tgaccggccg gagt
//
LOCUS       DBNGNK010000395         7623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_712725,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000395 DBNGNK010000000
VERSION     DBNGNK010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 7623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7623
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_712725"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC150V_11730"
     CDS             <1..350
                     /locus_tag="AC150V_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763630.1"
                     /translation="IVHELDLELDLDASAPSLVALAAVMTDEEPPAELERRSAAAAAA
                     AWDDGLRGALGDAPAYRRQAERDASVAVDVAPHELALPARESALARAAVDLAAQRPDS
                     AQDVVPVCRPSAR"
     gene            complement(347..787)
                     /locus_tag="AC150V_11735"
     CDS             complement(347..787)
                     /locus_tag="AC150V_11735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF047262.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HIR3763631.1"
                     /translation="MKPAATWLEPWPILAGPHPTVAEARRLAEDGITLFLDLTEPGEE
                     EPYAHAVERHLRISIEDHTAPEDESVISMALDAIDAEIGADGAVYVHCWWGCGRTGLV
                     LGCWLRRLGHDSEDALARIATLRGPGCPEMLVQVETVRAWAPDR"
     gene            complement(784..957)
                     /locus_tag="AC150V_11740"
     CDS             complement(784..957)
                     /locus_tag="AC150V_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763632.1"
                     /translation="MRVVNGAAGPLVELRLVSPAAARTLGVPIGLRRLAVAVDHPERL
                     LTELGAVAGEELS"
     gene            1074..1673
                     /locus_tag="AC150V_11745"
     CDS             1074..1673
                     /locus_tag="AC150V_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR3763633.1"
                     /translation="METEQVIVPLPVDAPLWERVHTVAPLVLVGTLEPDGSPDLAPKH
                     MAMPLGWSDRFCFVCTPRHSTYGNAVERGAFTVSFPTPAQVVGTSLAATLREADGSKP
                     ALAALSTIPAQEVEGVLVEGAYLFLECVFDRTVDFQDASLVIGRIVAAAADERALRSG
                     ERDDSDVVRTMPLLAYLPPGRFTVIDDSRAFPYPADFGV"
     gene            1663..2844
                     /locus_tag="AC150V_11750"
     CDS             1663..2844
                     /locus_tag="AC150V_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013304784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3763634.1"
                     /translation="MASDDALDGVDPAALLGWLRERTEEICHLLERLAAAESPSVVPE
                     SLRESYAILATEFEEVGYDVRPIPGERVGDHLYARPHGRRRSGGYQLLIGHMDTVWPV
                     GTLSVMPVRREGDVLYGPGVADMKGGLALMAWALRALAAHGVEPDITPVVLVTSDEEI
                     GSVESLPWLHRLAAGAVRAYVLEAPLGATGKLKTSRKGTGWFRVGVRGRAAHAGVDPE
                     AGHSAILELSHQIQRLFALNDPDTGITVNVGTIDGGLRPNVVAPEASAVVDVRVPTVA
                     AGERLEAAITGLEPVMPGVTIAIEGRMSRPPLEPTPRNRVLWHTAEKAAAMLGIPLEE
                     AAVGGASDGNYTSMHTATLDGLGAVGVGAHAHDERVLVPSLPERAALLALLLLAPREG
                     L"
     gene            2841..3896
                     /locus_tag="AC150V_11755"
     CDS             2841..3896
                     /locus_tag="AC150V_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HIR3763635.1"
                     /translation="MNEPVSQLPRQSTVLLPRGVVVGSIARNADFAGATVAPYPREEW
                     ATGDFVVCRVLDRLGRPHDIESTTGRMVEVISDDLVVGALGVRSATLEAMGDWRATGD
                     DLRMHALTRAGVMGLATSATPFARPKLVPLVYQGHVLLDGDKATMSGFVRHVDQLDLE
                     APVVIVIGTSMSAGKTTTVITVVRRLIRMGLRVAGTKVTGVARYREILGMADAGADPV
                     ADFVDAGLPSTICEPDVYRAALEHVLSRLAAQSPDVVVVECGASPLEPYNVQIAEDVL
                     RANVRCSILCASDPYAVVGVAQAHAVEPDLVAGRATSTDAGIALCERLAGVRGLNLLD
                     PDSHPALDQILADKLDL"
     gene            3992..4765
                     /locus_tag="AC150V_11760"
     CDS             3992..4765
                     /locus_tag="AC150V_11760"
                     /inference="COORDINATES: protein motif:HMM:NF037170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3763636.1"
                     /translation="MPVRSSYAPGTPCCADVSSPDIDSAVAFYGGLFGWSDFRAPEPE
                     AGGYTMFRQGEHDVAAVMTTQEGGPPPKWGTYISTDDVGAAAKKVRAAGGTVLVEPMD
                     VMEAGRMAVAQDSAGAVFCMWQPRQSGGATLVNEPVSMSWNELATRDAKAASAFYGEV
                     FGWKSEAMPDAPFEYHMQKVNGDPVAGIMEMPQEAADVPPNWMVYFAVEDTDDTIAKA
                     SELGGSLLNGPIETPFGPMAVLADPAGAVFSVIALTQTQ"
     gene            4884..5333
                     /locus_tag="AC150V_11765"
     CDS             4884..5333
                     /locus_tag="AC150V_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763637.1"
                     /translation="MGRTRAGRPASRADAGPVRDFDFWLGEWDVTWGDGETGTSSVYS
                     DFDDRVIVESFDGRPSSEVQGMSLSTFDDAAGVWRQAWVDSRGAFTVVSGSRTGDVME
                     LRTESGPLRRARWHDVTADSFTWTLERSSDGGASWETVRRLQYTRVL"
     gene            5390..>7623
                     /gene="dnaE"
                     /locus_tag="AC150V_11770"
     CDS             5390..>7623
                     /gene="dnaE"
                     /locus_tag="AC150V_11770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR3763638.1"
                     /translation="MGSPFVHLHVHSEYSILDGACRVPALAAKAATLGMPAVSLTDHG
                     SLAGAIDLYREARRQGIKPVIGCEVYVVDERSVQQKGHAHLTLLAASNEGYRNLIKLC
                     SLGYLEGYYYRPRVDWELLERHADGIIALSGCLSGRVSRAFAEHRPKDAEAELDRLVQ
                     LFGKDDVYVELQNAGLPIQQEILPQLVALAEHRSLPLVATGDVHYLDAGDAASHEALL
                     CIQSGDSLKNPNHWRFETNEFYFKSPDEMALDFPGHDEALRRTLEVAERCNVEIELGQ
                     ILLPAFPVPDDRDSFDYLVELCETGLARRYERVTPELNDRLRFELMTIREMGFADYFL
                     IVSDFVGFAKKNGIGVGPGRGSAAGSLVAYALEITDIDPIRYELLFERFLNPGRKSMP
                     DIDIDFAVEGRERVINYVAEKYGRDRVAQIITFSTMAARAAIRDAGRVLEVPYGTVDR
                     IAKLVPEGPGQTLEDALKPGAELRRAVDDDPIAKEIVELAQPLEGLTRADSIHAAGVV
                     IGAEPLIEVVPLQQKGGDQEVVTQLKMGDVEALGLLKMDFLGLRNLDVIDEAVRLVGG
                     SLDIASVPLTDTRTYEMLGRGESTGVFQFESSGMREALRQVRPTEFEDLIALVALYRP
                     GPMAYIPTYAARKNGREAVTFPDPRLEPITGPTYGICIYQEQYMEIAKQLAGFSPAEA
                     DDLRKAIGKKIHSLMASLKEKFIDGAVAKETTPAVARSLWEDMEKAQDYSFNKSHAAC
                     YALIA"
BASE COUNT         1145 a         2688 c         2621 g         1169 t
ORIGIN      
        1 gaatcgtcca cgagctcgac ctcgagctcg atctcgacgc gagtgcgcca tcgctcgtcg
       61 cactcgcggc cgtgatgacg gacgaggagc cgcctgcgga gctcgagcgc cggtccgccg
      121 ctgcggcagc ggcggcatgg gatgacggcc tgcgcggggc gttgggggac gcccccgcgt
      181 accgccgcca ggcggaacgc gacgcgtcgg tcgcagtcga cgtggcgccg cacgagcttg
      241 cgctcccggc acgcgagagc gcgctcgccc gcgccgcggt cgacctcgcc gcgcagcggc
      301 ccgacagtgc gcaggacgtc gtccccgtgt gtcgcccgtc ggctcgctag cggtcaggcg
      361 cccaggcgcg gacggtctcg acctgcacca gcatctcggg gcagccgggc ccgcgcagag
      421 tcgcgatgcg cgccagcgcg tcctcggagt cgtggcccag gcgcctcaac cagcacccca
      481 agacgagacc cgttcggccg cagccccacc agcagtggac gtagacggcc ccgtcggcgc
      541 cgatctcggc gtcgattgcg tccagggcca tgctgatgac cgactcgtcc tcgggagcgg
      601 tgtgatcctc gatcgagatc cggagatggc gctcgacggc gtgcgcgtac ggctcctctt
      661 cccccggctc cgtcagatcg agaaacagcg tgatgccgtc ctcggccagc cggcgggctt
      721 cagccaccgt ggggtgcggt ccggccagaa tgggccacgg ctcgagccag gtggccgcag
      781 gcttcacgaa agctcctccc cggccactgc tccgagctcc gtcagcaacc gctcgggatg
      841 gtcgacggcg acggccagcc gccgcagtcc aatcggtacg ccaagcgttc gtgccgcagc
      901 cggcgatacg agtcgtagct cgaccagcgg gccggccgct ccgttcacga cgcgcacgct
      961 tccagactag tttctccgac ggagacacgt tggcgtcccc tcccaacttc cacgcaggtc
     1021 gatcagtgct tccgccgatg ccggacgaag cgtccggccg ccagacttgg agggtggaaa
     1081 cagagcaggt gatcgttccg ctgcctgtcg acgcgccgct gtgggagcgc gtccacacgg
     1141 tcgcgccgct cgttctcgtc gggacgctcg agccggacgg ctcgcccgac ctggccccga
     1201 aacacatggc catgccgctc ggatggagcg accgcttctg cttcgtctgt acgccgcggc
     1261 actctacgta cgggaacgcg gtcgagcgcg gcgccttcac ggtcagcttc ccgacgcccg
     1321 cgcaggtcgt agggacgagc ctcgccgcga cgctgcggga agcggacggc tcgaagccag
     1381 cgctcgccgc gctgtcgacc attccggccc aggaagtcga gggagtgctc gtcgagggtg
     1441 cctacctctt cctcgagtgc gtgttcgatc ggacggtcga cttccaggac gccagcctcg
     1501 tgatcggccg catcgtcgcc gccgcagccg acgagcgtgc cctccggtcg ggcgagcgcg
     1561 acgactcgga cgtcgtccgc accatgcctc tcctcgcgta tctgcccccc ggacggttca
     1621 cggtgatcga tgacagccgc gcattcccgt atccagccga ctttggcgtc tgacgacgct
     1681 ctcgacggag tcgatcccgc cgcccttctg ggctggctcc gcgagaggac cgaagagatc
     1741 tgccatttgc tcgagcgtct cgctgcggca gagtcgccct cggttgttcc agagtcgctc
     1801 cgcgagtcgt acgcgatcct cgcgaccgag ttcgaggagg tcggatacga cgtccgcccg
     1861 atcccgggcg agcgtgtagg cgatcatctc tacgcccgtc cgcacggcag gcgccgcagc
     1921 ggcggctacc agctgctgat cggccacatg gacaccgtct ggccagtcgg cacgctctcc
     1981 gtgatgccgg tgcgccgtga gggcgacgtg ctctacggac cgggcgtcgc cgatatgaag
     2041 ggcgggctcg ccctcatggc ctgggcgctg cgcgcgctcg ccgcccacgg ggtggagccc
     2101 gacatcacgc cggtggtcct cgtcaccagc gacgaggaga tcggcagcgt cgaatcgctc
     2161 ccctggctgc accggctggc ggccggcgcg gtacgggcgt acgtgctcga agcgccgctc
     2221 ggcgcgaccg ggaagctgaa gacgtcgagg aaggggaccg gctggttccg cgtcggcgtc
     2281 cggggacgcg ccgcccatgc cggcgtcgat cccgaggccg gccactccgc catcctcgag
     2341 ctgtcccatc agatccagcg gctgttcgcg ctcaacgacc ccgacacggg gatcaccgtg
     2401 aacgtcggca caatcgacgg tgggctgagg ccgaacgtcg tcgcgccgga ggcgagcgcc
     2461 gtcgtcgacg tgcgcgttcc cacggtggct gcgggagagc gtctcgaagc ggcgatcacg
     2521 gggctggagc cggtcatgcc gggcgtcacg atcgcgatcg aggggcgcat gtcgcgaccg
     2581 ccgctcgagc cgacacctcg caaccgagtc ctctggcata cggccgagaa ggcggccgcc
     2641 atgctcggca tcccgctgga ggaggccgcg gtgggaggcg cctcggacgg caactacacg
     2701 agcatgcaca ccgccacgct cgacggtctc ggcgcggtcg gcgtcggtgc gcacgcgcac
     2761 gacgagcgcg tcctggtgcc gagcctgcca gagcgcgcgg ccctgctcgc gctcctcctc
     2821 ctcgctccgc gggaggggtt gtgaacgagc ccgtctcgca gcttccgcgc cagtcgacgg
     2881 tgctcctgcc gcgcggcgtc gtcgtcggca gcattgcgcg gaacgccgac ttcgcgggtg
     2941 ccaccgtcgc cccgtatccg agggaagaat gggcgaccgg cgacttcgtc gtctgtcgcg
     3001 tcctcgatcg gctgggccga ccgcacgaca tcgagtcgac gacgggcagg atggtcgagg
     3061 tgatctccga cgacctcgtc gtcggggcgc tcggcgtgcg cagcgcgacc ctcgaggcga
     3121 tgggcgactg gcgagcgacg ggcgacgacc tgcggatgca cgcgctcacg cgcgccgggg
     3181 tgatgggcct ggcgacgtcg gcgacaccgt tcgcgcgccc caagctcgtg ccgctcgtct
     3241 accagggtca cgtccttctg gacggtgaca aggcgacgat gagcggcttc gtgcgccacg
     3301 tcgaccagct cgatctcgag gcccctgtcg tgatcgtgat cggcacctcg atgagcgcgg
     3361 ggaagaccac gaccgtgatc accgtcgtcc ggcgcctgat ccggatgggg cttcgggtcg
     3421 ccgggacgaa ggtgaccggt gtggcgcgct accgcgagat cctcggcatg gcggacgcag
     3481 gggccgatcc cgtcgccgac ttcgtcgacg ccgggctccc gtccacgatc tgcgagcccg
     3541 acgtctatcg ggctgcgctc gagcacgtgc tctcgcggct ggcagcccag agcccggacg
     3601 tcgtcgtcgt cgagtgcggc gcgtcgcccc tcgagccgta caacgtccag atcgccgagg
     3661 acgttctccg cgcgaacgtg cgctgctcga tcctgtgcgc gtcggacccg tatgccgtcg
     3721 tgggcgtcgc gcaggcccac gccgtcgagc ccgacctcgt cgcgggccgg gcgacatcga
     3781 ccgacgccgg gatcgcgctg tgcgagcggc tggccggcgt ccgcgggctc aatctcctgg
     3841 atcccgactc ccaccccgcg ctcgaccaga tcctggccga caagctcgac ctctagattc
     3901 ccggccgcca gctagacctt cacccaaccg caacctcgcg ctcgttatcg tcggcaccgt
     3961 tgctcccccc cgacccgaaa ggagcctcac gatgccagtt cgttcttcct acgcccctgg
     4021 gacaccgtgt tgtgccgacg tctcctcccc agacatcgac tcggcggtcg ccttctacgg
     4081 tgggctcttc ggctggagcg acttccgcgc gccggagccc gaggcaggcg gctacacgat
     4141 gttccggcag ggcgagcacg acgtcgccgc cgttatgacg acgcaggagg gcgggccgcc
     4201 gccgaagtgg ggcacctaca tctcgaccga cgacgtgggc gccgccgcaa agaaggtccg
     4261 cgctgcgggc ggtacggtgc tcgtcgagcc gatggatgtg atggaggcgg gtcggatggc
     4321 ggtcgcgcag gacagcgcgg gcgcggtgtt ttgcatgtgg cagccaaggc agagcggtgg
     4381 cgcgacgctc gtcaacgagc ccgtcagcat gtcctggaac gagctcgcca cgcgtgacgc
     4441 caaggctgcg agcgccttct acggcgaggt cttcggctgg aagtcggagg cgatgcccga
     4501 cgcccccttc gaataccaca tgcagaaggt caacggagac ccggtggcgg gcatcatgga
     4561 gatgccgcag gaagcggcgg acgtgccccc caactggatg gtctacttcg cggtcgagga
     4621 caccgacgac acgatcgcga aggcctcgga gctcggtggc tcgctcctca acgggccgat
     4681 cgaaacgccc ttcggaccca tggctgtcct cgccgacccg gcgggcgccg tgttctcggt
     4741 gatcgcgctg acacagacgc agtgaccgct cggctggacg gcgacaacgc cgtcgccctc
     4801 gtcgcccgtt aagcgcgtgt tgcggagcgg ttgcggctgc gtgaatacgc tgtcagatcg
     4861 tcacttcgag cgtctgaacc ggcatggggc ggacgcgggc agggcggcct gcctcccggg
     4921 ccgatgccgg gcctgttcgc gacttcgact tctggctcgg cgaatgggac gtcacctggg
     4981 gtgacggcga gaccggcacg agctccgtct actccgactt cgacgatcgg gtgatcgtgg
     5041 agagcttcga cggccggccc tcgtccgagg tccagggcat gtcgctgtcc acgttcgacg
     5101 acgcggccgg cgtgtggcgc caggcctggg tcgacagccg cggcgcgttc accgtggtgt
     5161 cgggctcgcg cacgggtgac gtgatggagc tgcgaacgga gagcggcccg ctgcgacgcg
     5221 cccgctggca cgacgtcacg gcggactcgt tcacctggac gctcgagcgc tcgtccgacg
     5281 gtggggcgag ctgggagacc gtccgccggc tccagtacac gcgggttctc tgacgcgact
     5341 ggccgcgccc ggtgaggccg tcgggcctga gcagtacgat ggccctcccg tgggttcgcc
     5401 cttcgtccac ctccacgtcc actccgagta ttcgatcctc gacggggcct gccgagtccc
     5461 cgcgctcgcc gcgaaggcgg cgacgctggg gatgccggcc gtctcgctca ccgatcacgg
     5521 ctcgctggcg ggggcgatcg acctctaccg cgaggcgcga cggcagggca tcaagccggt
     5581 catcggctgc gaggtgtacg tcgtcgacga gcgctcggtg caacagaagg ggcatgctca
     5641 tctgaccctg ctcgccgcgt cgaacgaggg gtaccggaat ctgatcaagc tctgctcgct
     5701 cgggtatctc gagggctact actaccgtcc gcgcgtggac tgggagctgt tggagcggca
     5761 cgcagacggc atcatcgccc tctcgggctg cctctccggc cgcgtgagtc gcgctttcgc
     5821 cgagcaccgg cccaaggacg cggaggccga gctggaccgg ctcgtgcagc tgttcgggaa
     5881 ggacgacgtc tacgtcgagc tgcagaacgc cggcctgccg atccagcagg agatcctgcc
     5941 ccagctcgtc gcgctcgccg agcatcggtc gctgccgctc gtcgccacgg gagacgtgca
     6001 ctacctggac gccggcgacg ccgcctcgca cgaggcgctc ctgtgcatcc agtccggcga
     6061 ctcgctgaag aacccgaacc actggcgctt cgagacgaac gagttctact tcaagtcacc
     6121 ggacgagatg gccctcgact ttcccggcca cgacgaagcg ttgcgccgga cgctcgaggt
     6181 cgccgagcgc tgcaacgtcg agatcgagct cgggcagata ctgctgccgg ccttcccggt
     6241 gccggacgac cgcgactcgt tcgactacct cgtcgagctc tgcgagacgg gcctcgcccg
     6301 ccgctacgag cgcgtgacgc ccgagctgaa cgatcggctt cgcttcgagc tgatgacgat
     6361 ccgtgagatg ggcttcgccg actacttcct gatcgtctcc gacttcgtcg gcttcgcgaa
     6421 gaagaacggc atcggcgtcg gccccggccg ggggtccgcg gcgggctcgc tcgtcgccta
     6481 cgccctcgag atcaccgaca tcgacccgat ccgctacgag cttctcttcg agcgcttcct
     6541 caaccccggc cgcaagtcga tgcccgacat cgacatcgac ttcgctgtcg aggggcgcga
     6601 gcgggtcatc aactacgtcg ccgagaagta cggccgcgac cgggtcgcgc agatcatcac
     6661 cttctcgacg atggctgcgc gtgcggcgat ccgcgacgcc ggaagggtgc tcgaggttcc
     6721 ctacgggacc gtcgaccgga tcgccaagct cgtgccggag ggtccggggc agaccctcga
     6781 ggacgcgctc aagcccggcg ccgagctgcg acgcgcggtc gacgacgacc cgatcgccaa
     6841 ggagatcgtc gagctcgcgc aaccgctcga gggtctcacc cgggcggact cgattcatgc
     6901 ggcaggcgtc gtgatcggcg cagagccgct gatcgaggtc gtcccgctcc agcagaaggg
     6961 cggcgaccag gaggtcgtca cccagctgaa gatgggagac gtcgaggcgc tcggcctgct
     7021 gaagatggat tttctcggcc tgcgcaacct cgacgtgatc gacgaggccg tgcgactcgt
     7081 cggtgggtca ctggacatcg cctcggtccc gctgaccgac acgcgaacgt acgagatgct
     7141 cggccgagga gagtcgaccg gcgtcttcca gttcgagtcg tccggaatgc gcgaggcgct
     7201 ccggcaggtg cgccccaccg agttcgaaga cctgatcgcc ctcgtcgccc tctatcgccc
     7261 gggcccgatg gcctacatcc ccacctacgc cgcgcgcaag aacgggcgcg aggcagtcac
     7321 cttccccgat ccgcggctcg agccgatcac cggccccacg tacgggatct gcatctacca
     7381 ggagcagtac atggagatcg cgaagcagct cgccggcttc tcgccggccg aggcggacga
     7441 tctccgcaag gcgatcggaa agaagattca ctcgctgatg gcatccctga aggagaagtt
     7501 catcgacggc gctgttgcca aggagacgac accggccgtg gcccggtcct tgtgggaaga
     7561 catggagaag gcgcaggact actcgttcaa caagtcgcac gcagcctgct acgcgctgat
     7621 cgc
//
LOCUS       DBNGNK010000396         3203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_427357,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000396 DBNGNK010000000
VERSION     DBNGNK010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_427357"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(287..3187)
                     /gene="carB"
                     /locus_tag="AC150V_11775"
     CDS             complement(287..3187)
                     /gene="carB"
                     /locus_tag="AC150V_11775"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR3763639.1"
                     /translation="MTDPGVADRTYIEPLDLEGVADVLRVERPDAVLPTLGGQTALNL
                     AIALHESGILAELGVELLGARVDAIRRAEDREQFRAAVQSCGLHVPESMIVTSSAELE
                     DVAVPAVVRPAFTLGGHGGGFAETSEQLHRQVERGLRESPIGQVLVEESVRGWDEFEL
                     EVMRDRNDNVVVVCSIENLDPMGVHTGDSVTVAPQMTLPDEAYQELRDAAAAVIRAVG
                     VETGGSNIQFARSRETGDVRVIEMNPRVSRSSALASKATGYPIAKVAAKLAVGYTLDE
                     IPNDLTKTTPASFEPTLDYVVVKFPRFAFEKFPGADRALGTQMKSVGEAMGIGRTFTE
                     AFLKAFGSRELEPGAPTPWATLDDVPEGVHPWFLDQLEYAKRELASRDLVRAKRAVWG
                     DDSIAGAWGTNGEDVRHQRYALGVRPSYRRVDSCGGEVAAESNYYYSTWGEEDESLPP
                     ATRPRVTIIGSGPNRIGQGIEFDYCCVHAAQTFRALGYEAVMVNCNPETVSTDYDTSD
                     RLYFEPLSPEEVLAVLDREQPDGVVTQFGGQTPLRLARWVEAAGFKIMGTPHAAIDLA
                     EDRERFGALLGELGLRCPPWAIVQGPDQALAAAREIGYPALVRPSYVLGGRAMRVCYG
                     EEQLREAIEAVRGPVLLDRFIENAIEIDVDALCDGDDVYIGAVMQHVEEAGVHSGDSA
                     CVLPPQALTLSNALEVEHVVRRLGPALGVVGLLNVQLAVADATVYILEANPRASRTVP
                     FASKATGVNLVEAACRIAVGATIGELELPPERPPTEVSVKAAVLPFSRFPGADPVLGP
                     EMRSTGEVMASARDLPTAFAKAERAAGRPLPNSGTAFLSVRDSDKPAVVPVAAALAGL
                     GFGLLATEGTATTLRAAGLAVEEIAKVAQAAEEEETVVDLVRRGRCDLIVNTPQGSGA
                     RADGYLIREAALVARVPCITTLSGAAAAVHAIANARTERALSLQERHAATPS"
BASE COUNT          480 a         1158 c         1112 g          453 t
ORIGIN      
        1 ctgcaaagcg ctcggcttcc cggaccgatc ggatcgatca ggaaggccag ctcgcccgga
       61 ggcgccaggc agagcgacat cggacgcggc agcacccgcc ccggcgcctc gagcatgaag
      121 aactgcccgg gctccccagg ctcgactttc ccgcgacgca cgcggagtag cgtgtaccgg
      181 ccgactcgct ccgcgcccga gaccgagagg cgctgccgcc tgctccggca ggatgcgctc
      241 aagccgggcg ccgggactcg ggccggccca tcttcggcgg ctcgcgtcac gacggcgtcg
      301 cggcgtggcg ttcctggagc gagagggcgc gctcggtgcg cgcattcgcg atcgcgtgga
      361 cggcggccgc ggcacccgag agcgtcgtga tgcacggaac gcgagcgacg agcgccgcct
      421 cgcggatgag atacccgtcg gcgcgggcgc ccgagccctg cggggtgttc acgatgaggt
      481 cgcagcggcc gcggcgcacg aggtcgacga ccgtctcctc ctcctctgcc gcctgcgcga
      541 ccttggctat ctcctcgacg gccagccccg ccgcccgcag cgtcgttgcc gtgccctcgg
      601 tggcgagcag cccgaagccg aggccggcca gcgcggctgc gaccgggacg acggcgggct
      661 tgtccgagtc gcgaacggac aggaacgccg tacccgagtt cggcaagggg cgccctgccg
      721 cgcgctccgc cttcgcgaag gccgtcggca gatcgcgggc gctcgccatc acttcgccgg
      781 tcgagcgcat ctcgggaccc agaaccgggt ccgcgccggg aaagcgcgag aacggcagca
      841 cggcagcttt tacgctgacc tccgtcggag ggcgctcagg ggggagctcc agctcgccga
      901 tcgtggcgcc gaccgcgatc cgacaggcag cctccacgag gttcacgccc gtcgccttgc
      961 tggcgaacgg caccgttctg gatgcgcgcg gattggcctc caggatgtag acggtcgcgt
     1021 cggccacggc gagctgaacg ttgagcagcc cgacgacgcc gagcgccggc ccgagccggc
     1081 ggacgacatg ctcgacctcg agggcattcg agagcgtgag cgcctgcggc ggcaacacgc
     1141 acgcagagtc tcccgagtgg acgccggcct cctcgacgtg ctgcatcacc gcgccgatgt
     1201 acacgtcgtc gccgtcgcag agcgcgtcga catcgatctc gatcgcgttt tcgatgaatc
     1261 ggtcgaggag caccgggcca cgcaccgcct cgatcgcctc acggagctgc tcctccccgt
     1321 agcagacgcg catcgcgcgt ccgccgagga cgtaagacgg acggacgagc gccgggtagc
     1381 cgatctcgcg ggcggcggca agagcctgat cgggcccctg cacgatcgcc cagggcgggc
     1441 agcggaggcc gagctcgccc aggagcgctc cgaaccgctc gcggtcttcg gcgaggtcga
     1501 tggcggcatg aggagtcccc atgatcttga agcccgcggc ctcgacccag cgcgcgaggc
     1561 gcagcggcgt ctggccgccg aactgcgtga ccacgccgtc gggctgctcg cgatcgagga
     1621 ctgccagcac ctcctcgggt gagagcggct cgaagtacag gcgatccgat gtgtcgtagt
     1681 cggtcgagac cgtctcgggg ttgcagttga ccatcacggc ctcgtagccg agcgcacgga
     1741 aggtctgcgc cgcatggacg cagcagtagt cgaactcgat cccctgcccg atccggttcg
     1801 ggccggagcc gatgatggtc acgcgcggcc gggtcgcggg cggcaggctc tcgtcctctt
     1861 ccccccacgt cgagtagtag tagttggact ccgccgccac ctcgccgccg caggagtcga
     1921 cccgccggta gctggggcga acgccgagcg cgtagcgctg atggcgcacg tcctcgccgt
     1981 tcgtgcccca ggcgccggcg atcgagtcgt cgccccaaac ggcccgcttg gcgcgcacga
     2041 gatcgcggga ggcgagctct cgcttcgcgt actcgagctg gtccaggaac cacggatgca
     2101 cgccctcggg aacgtcgtcg agcgtcgccc acggagtcgg cgcgccgggc tcgagctccc
     2161 gcgagccgaa cgccttcagg aacgcctcgg tgaacgtccg cccgatgccc atcgcctcgc
     2221 ccacggactt catctgcgtg ccgagggcgc ggtcggcgcc ggggaacttc tcgaaggcga
     2281 agcgcgggaa cttgacgacc acgtagtcca gggtgggctc gaagctcgcc ggggtcgtct
     2341 tcgtcagatc gttcgggatc tcgtcgagcg tgtagcccac cgccagcttg gcggccacct
     2401 tcgcgatcgg gtaccccgtc gccttcgacg cgagcgccga ggagcgcgat acacgcgggt
     2461 tcatctcgat tacccgcacg tcgccggtct cgcgggagcg cgcgaactgg atgttcgacc
     2521 ccccggtctc gacgccgaca gctcggatca ccgccgcggc agcgtcgcgc agctcctgat
     2581 aggcctcgtc gggcagcgtc atctgaggcg cgaccgtcac cgagtcaccg gtgtgcacgc
     2641 ccatcgggtc gaggttctcg atcgagcaca caaccacgac gttgtcgttg cgatcccgca
     2701 tcacctcgag ctcgaactcg tcccagccgc ggaccgactc ctccaccagc acctgcccga
     2761 tcgggctctc gcgcagaccg cgctcgacct gccggtgcag ctgctccgac gtctcggcga
     2821 accccccgcc gtggccgccc agcgtgaacg cgggacggac gacggccggt accgccacgt
     2881 cttccagctc ggcgctggag gtgacgatca tcgattcggg gacgtgcaat ccgcagctct
     2941 ggacggcggc gcgaaactgc tctcgatcct cggcgcggcg aattgcgtca acgcgcgctc
     3001 ccagcagctc gacgccgagc tcggcgagga tcccgctctc gtgcagcgca atggcgagat
     3061 tcagcgccgt ctgaccgccc aacgtcggga ggacggcgtc gggtcgctcg acgcgcagca
     3121 cgtcggcgac gccttcgaga tcgagcggct cgatgtacgt gcggtcggcg acgccggggt
     3181 cggtcatgat cgtcgccgga ttc
//
LOCUS       DBNGNK010000397         3529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_570713,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000397 DBNGNK010000000
VERSION     DBNGNK010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_570713"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1603
                     /locus_tag="AC150V_11780"
     CDS             <1..1603
                     /locus_tag="AC150V_11780"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR3763640.1"
                     /translation="IGIAVFLAGAVLLGLEIVASRVVAPFFGSSLFVWGALIGVVLAG
                     LSIGYWIGGALADRFPTPYLLIGVLAVSAGLVLAIPLIDEWVLERVVAWDPGPRANPL
                     VATILLFGLPSVILGGVSPIAVKLVARSLERVGRTAGRLFAVSTAGSIAGTFATAFWL
                     VPELGTDQVLAVGALALLVAASVVALGQRLLAPTFGLAAAAAGAVVIVIALAPDTGGT
                     LEGAAAKNWSPLYRLREAQTPSQLDPASVSADGFTVREAKETRYHRLVVADDEESRYL
                     RFDSSFQSGMYLDDPYKTRFTYTDYLNLGVAYKPDAKRVLFIGLGGGSAPKRMWRDFP
                     GMEITAVELDPDVAEAAYRWFKLPRDERLRVESGDGRRFLAANEERWDVIAIDAFFSD
                     AIPFHMATREFVELAAERLEPGGVIVTNVIGSVTGESSRLLRSLARTYATVFPTVAAH
                     PVYFKGDEDPESIRNVILLATATPLPTKAVLQTRWDAVRAAATSAPNLTRAINDRRDA
                     EIQTNDVPLLTDDYAPTDALLLLFG"
     gene            complement(1698..2480)
                     /locus_tag="AC150V_11785"
     CDS             complement(1698..2480)
                     /locus_tag="AC150V_11785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR3763641.1"
                     /translation="MALDLDGTLLRSDRSVSPRTRGALARAQATGAHIVLVTARSPRS
                     ARDIAADLALDGWAVCANGATIYDLDEDAIVEHRPLDTGVAHALVRSLRELVEGVVFG
                     WESEMTFGSEPDYELLRSPEWPRPEGSFPPADPLSFPGPFTKVIARSPVTELHDLHAT
                     AVGVARGEAEVTLAGDAFVELLDREATKGNALERLAARLGVAAADAVAFGDQLVDISM
                     LAWAGHSVAPANARPEVRQAAAEVSPSNDEDGVALVLERLFA"
     gene            complement(2505..3527)
                     /locus_tag="AC150V_11790"
     CDS             complement(2505..3527)
                     /locus_tag="AC150V_11790"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3763642.1"
                     /translation="MAVLGILTAGGDCPGLNAVIRAVVARATAGSETEVLGILDGWDG
                     LLTGRTRRLDRDDVRGILQRGGTILGTSRRDPYVHADGYESVRRTIVRNGIEALVVVG
                     GDGTLRTALRLQEEGLQIVGVPKTIDNDIAGTDVTFGFNTAVQIATDAIDRLTTTAEA
                     HDRIMLVEVMGRSKGWIAVYAGIAAGVDAILIPEVPYDLTEITRIIQSRHRGGRHYSV
                     VVVAEGVTPPDGDVPSERDAFGFERLGGVAHRLAPVLEEMTGFETRVTVLGHIQRGGT
                     PTAFDRVLATRFGTAAADLAAAGRFGTMVAARGTQIVAVPLAEACAETRGVPRELHDV
                     AKTFFG"
BASE COUNT          493 a         1261 c         1220 g          555 t
ORIGIN      
        1 gatcggaatc gccgtcttcc tcgcgggtgc ggttctcctc ggcctcgaga tcgtcgcgag
       61 ccgtgtcgtc gctcccttct tcgggagctc gttgttcgtc tggggcgcgc tcatcggcgt
      121 cgtgctcgca gggctctcga tcggctactg gatcggtggc gctctggccg atcgcttccc
      181 gacgccgtac ctgctgatcg gtgtgctggc cgtctcggca ggactcgtcc tcgccatacc
      241 tctgatcgac gagtgggtgc tcgaacgggt cgtcgcctgg gatcccgggc cgagggcgaa
      301 cccgctcgtg gcgacgatcc tgctcttcgg cctgccgtcg gtcatcctag gcggcgtctc
      361 gccgatcgcc gtgaagctcg tcgcgcggtc gctggagcgg gtcgggcgga cagccggcag
      421 gctgttcgcc gtctccaccg ccgggagcat cgccgggacg ttcgctaccg ccttctggct
      481 cgtccccgag ctcggcaccg accaggttct cgccgtgggc gcgctcgcct tgctcgtggc
      541 ggcgtcggtg gtcgcactcg ggcagcgtct cctggcgccc accttcgggc ttgcggccgc
      601 cgccgcaggc gcagtcgtga tcgtgatcgc actcgctccg gacacgggag gaacgctcga
      661 gggtgctgct gcgaagaact ggtcgccgct ctaccgcctg cgcgaggcgc agacgccgtc
      721 ccagctcgat ccggcctcgg tctcggccga cggcttcacc gtccgggagg cgaaggagac
      781 gcgctaccac cggctcgtcg tcgccgacga cgaggagtca cgctacctcc gcttcgacag
      841 ctcgttccag agcggcatgt acctcgacga tccgtacaag acgcgcttca cctacaccga
      901 ctacctgaac ctcggagtcg cctacaagcc cgatgcgaag cgcgtgctct tcatcggttt
      961 gggaggtggg tcggcgccga agcgcatgtg gcgcgacttc cccggcatgg agatcacggc
     1021 ggtcgagctc gaccctgacg tcgcagaggc cgcgtaccgg tggttcaagc tgccgcggga
     1081 cgagcggctg cgcgtcgagt ccggcgacgg gagacgcttc ctcgccgcca acgaggagcg
     1141 ctgggacgtg atcgcgatcg atgcgttctt ctccgacgcg atcccctttc acatggccac
     1201 gcgcgagttc gtggagctgg ccgccgagcg gctggagccg ggcggcgtga tcgtgacgaa
     1261 cgtgatcgga tccgtgaccg gcgaatcgtc gcggctgctc cgctcgctgg cccgcaccta
     1321 tgccacggtc ttcccgaccg tggctgctca ccccgtctac ttcaagggcg acgaggatcc
     1381 cgagtcgatc cgcaacgtga tcttgctcgc gaccgcgacg cctcttccga cgaaggccgt
     1441 gctgcagacg cgctgggacg cggtgcgcgc agcggcgacg agcgcaccca acctcacccg
     1501 cgccatcaac gaccgccgcg acgccgagat ccagacgaac gacgtgccgt tgctgacgga
     1561 cgactacgcg ccgaccgacg cgctcctgct gctcttcggc tgacggtcga ccctcacggc
     1621 gagcctgttg gcaaccgccg gctttgcccc ctggtacagc cgccctctgc cgcgacgacg
     1681 ccagcgcgcc cgtctgtcta ggcgaacagc cgctcgagca cgagggccac gccgtcctcg
     1741 tcgttcgacg ggctcacctc ggcggccgcc tgcctgacct ccgggcgcgc gttcgcgggc
     1801 gcgacggagt ggccggccca ggcgagcatc gagatgtcga ccagctggtc gccgaacgcc
     1861 acggcgtcgg ctgccgccac accgagtcgg gccgcgagtc gctcgagggc gtttcccttc
     1921 gttgcttccc gatcgagcag ctcgacgaac gcgtcgcccg cgagcgtgac ctcggcctcg
     1981 ccccgagcaa cgccgacggc ggtcgcgtgc aggtcgtgca gctcggtgac cggtgagcgc
     2041 gcgatcactt tcgtgaacgg ccccggaaac gaaagcgggt cggcaggcgg gaacgagccc
     2101 tcgggcctgg gccattcggg agagcgcagc agctcgtagt cgggctcgct gccgaacgtc
     2161 atctccgact cccagccgaa gacgacgcct tcgaccagct cccgcagcga ccgcacgagc
     2221 gcatgcgcga cgccggtgtc gagcggccgg tgctcgacga tggcgtcctc atcgaggtcg
     2281 tagatcgtcg ccccgttcgc gcacaccgcc cagccgtcga gagcaaggtc ggccgcgatg
     2341 tcccgagcgc tgcgcgggga gcgggcagtg acgagcacga tgtgcgcccc ggtcgcctgc
     2401 gcgcgagcga gggcgccgcg ggtccgcggc gagacgctgc ggtcggagcg gagcagcgtt
     2461 ccgtcgaggt cgagcgccac gagccgaggg gcggaagaca cggtctagcc gaagaacgtc
     2521 ttcgccacgt cgtgcagctc ccttgggacg ccccgagtct cggcgcaggc ctccgcgagg
     2581 gggacggcga cgatctgggt gccgcgggcg gccaccatcg tcccgaagcg gccagcggcg
     2641 gcgaggtccg cagccgccgt gccgaagcga gtcgcaagca cgcggtcgaa ggcagtcggt
     2701 gtgccgccgc gctggatgtg cccgagcacg gtcacgcgcg tctcgaagcc ggtcatctcc
     2761 tcgagcacgg gcgcgaggcg atgcgcgacg ccgcctaggc gctcgaagcc gaacgcgtcg
     2821 cgctccgacg gcacgtcccc gtcagggggc gtcacgcctt ctgcgacgac aacgacggag
     2881 tagtgccgcc cgccgcggtg gcgagactgg atgatccgcg tgatctcggt gaggtcgtac
     2941 ggcacctcgg ggatcaggat cgcgtccacg cccgcggcga tcccggcgta caccgcgatc
     3001 cagcccttcg aacggcccat cacctcgacc agcatgatcc gatcgtgtgc ctcagcggtc
     3061 gtggtcagcc ggtcgatggc gtccgtcgcg atctgaaccg ccgtgttgaa gccgaacgtc
     3121 acgtcggtgc ccgcgatgtc gttgtcgatc gtcttcggca ctccgacgat ctggagtccc
     3181 tcctcctgga ggcggagcgc ggtgcggaga gttccgtcgc cgcctaccac gaccaatgcc
     3241 tcgatgccgt tccgaacgat cgtcctccgt acgctctcgt agccgtcggc gtggacgtac
     3301 gggtcgcgtc tggaggtgcc gaggatcgtg ccgccgcgct ggaggatgcc tctcacgtcg
     3361 tcgcgatcga gccggcgcgt ccgcccggtc aggagcccgt cccagccgtc gagaatgccg
     3421 aggacctcgg tctccgagcc ggccgtggca cgggcgacga ccgcacggat gacggcgttc
     3481 agcccaggac agtccccgcc cgcggtgagg atccccagga cggccattt
//
LOCUS       DBNGNK010000398         3751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_499456,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000398 DBNGNK010000000
VERSION     DBNGNK010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_499456"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1173
                     /locus_tag="AC150V_11795"
     CDS             <1..1173
                     /locus_tag="AC150V_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012341.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enolase-like domain-containing protein"
                     /protein_id="HIR3763643.1"
                     /translation="YADPRSSGKYEINHFPAGGVGGSIDCINSTLRGELVGYDAADQE
                     GIDRLLVDLDGTERFEVIGGNTAEAVSMAGAKAAAASLGVPLYRYAAVDGAPAVPHQM
                     PNIIGGGATMGDAGWRGRTPDLQDHVVLPVGCGSTYEEMERVAEVFHRVGALLREADP
                     HFAGGRDEEYCWLPGLDDVVCLEVLERACAQVAGNHGIAFRLGLDVGAADLWDESEEA
                     YLYAREGVKRTRGEHAAHLARLIERFDLFYVEDAFFEDHVELYVEQMESFGGRLLVAG
                     DDLLAGDLDRLERMAGLGAVNAAVVKLNMAGTVTRTRRFVEACRDTGLATIGSCRTYD
                     SPDDTLADLVVAWGSNVYKCGSPAGGEHAAKYNRYVRIDEELGEAPSFAEFPGVGP"
     gene            1170..2147
                     /locus_tag="AC150V_11800"
     CDS             1170..2147
                     /locus_tag="AC150V_11800"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR3763644.1"
                     /translation="MSDVLYLAPRHLDSLDLDPTSVVDLLEEVFRHKARGAAEMPPKI
                     FFQMEGDRFYSAMASCCPPLGFAGCKWQSGDPANPSRGMPYVQGLFVLSEHETGRMVA
                     VIDAGWITGKRTAAASALVARYQAREGAEELALLGCGLQGRAHLRALAAELPGLSRCR
                     VFDVVPEVQTAFVEDLDGRCNGLRVRGAADAESAVRGADVVIPGGPIRVRRQPTIVSD
                     WVEPGALVVTIDYDSYVTDECIAAMDLVLTDDRGQIESARRTEGKFLGVDRIDAEIAE
                     MVATGRGRRQTDEQRILSFNLGIALEDVAVAAEVYRRAREGGAGIPLRP"
     gene            2203..2589
                     /locus_tag="AC150V_11805"
     CDS             2203..2589
                     /locus_tag="AC150V_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763645.1"
                     /translation="MGGNLRFAAFIVVAFVVFVFVLRLALRARSRRPPAGRVYGTALV
                     VVVGGMLFARLGAQAGQPWWIYYTLPALVTILVPPVVFRMDRRETLVYLVLAFTSSPL
                     IHVVFSFFLGWKEYMPFLEVPAIWEL"
     gene            2803..3024
                     /locus_tag="AC150V_11810"
     CDS             2803..3024
                     /locus_tag="AC150V_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763646.1"
                     /translation="MNLKLNGAFRGEVNHSAWVDQIRSIPSRLCPSEEGLALFAAQPL
                     AVEGSSYAHVVKGCDRCRERLQQLLLSSS"
     gene            complement(3134..3571)
                     /locus_tag="AC150V_11815"
     CDS             complement(3134..3571)
                     /locus_tag="AC150V_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009583435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR3763647.1"
                     /translation="MRHIQRASWVVLALVLAAAASAQSDHEAVERAVLDYVEAVEQGR
                     PELIERGVHPELAKFGFYREAPGSEYEIYPMTFEALVELAANMKKEGHVPDDATHSVE
                     IFEVLDQTASVKLTAFWGIDYMHLAKYDGEWKIVQVLWQSPPP"
BASE COUNT          545 a         1259 c         1333 g          614 t
ORIGIN      
        1 tacgccgatc cgcgcagctc cgggaagtac gagatcaacc actttccggc cggaggggtg
       61 gggggctcga tcgactgcat caactcgacc ctgcgcggcg agctcgtcgg gtacgacgcc
      121 gcggaccagg aggggatcga ccggctcctc gtcgacctcg acggaacgga gcgcttcgag
      181 gtgatcggcg gcaacaccgc cgaggccgtc tcgatggcgg gcgccaaggc ggcggccgcc
      241 agcctcggcg tgccgctcta tcgctatgcc gccgtcgacg gcgcaccggc ggttccgcac
      301 cagatgccca acatcatcgg gggcggcgcc accatgggcg acgcgggctg gaggggcagg
      361 acgcccgacc tccaggacca cgtcgtcctg ccggtcggct gcggatccac ctacgaagag
      421 atggagcggg tcgcggaggt cttccaccgc gtcggtgcgc tgctgcggga ggccgatcct
      481 cacttcgcgg gcggacggga cgaggagtac tgctggttgc ccggactcga cgacgtcgtc
      541 tgcctggagg tcctcgagcg agcgtgcgcg caggtggcgg gaaaccacgg tatcgccttt
      601 cgcctcggcc tcgacgtcgg cgcggccgat ctctgggacg agtccgaaga ggcgtacctc
      661 tatgcccgcg agggcgtcaa gcggacgcgc ggcgagcacg ccgcgcacct tgcccgcttg
      721 atcgagcgct tcgacctctt ctacgtcgag gacgccttct tcgaggatca cgtcgagctc
      781 tacgtcgagc agatggagag cttcgggggc cgtctgctgg tcgcgggcga cgatctgctg
      841 gcgggcgatc tcgaccggct cgagcggatg gccgggctgg gagccgtcaa cgccgcggtg
      901 gtcaagctca acatggcggg caccgtcacg cggacgcgac gattcgtcga ggcgtgccgg
      961 gacacgggcc tcgccaccat cggctcctgt cgcacctacg acagccccga cgatacgctg
     1021 gcggatctcg tcgtcgcgtg gggctcgaac gtctacaaat gcggctcgcc ggcgggaggc
     1081 gagcacgccg ccaagtacaa tcgctacgtc cgtatcgacg aggagctggg cgaagctccg
     1141 agcttcgcgg agtttcccgg ggtgggacct tgagcgacgt tctctatctc gcccctcgtc
     1201 acctcgactc gctcgatctc gacccgacgt cggtcgtcga tctgctcgag gaggtcttcc
     1261 gccacaaggc gcgcggcgcc gcggagatgc cgcccaagat cttctttcag atggagggcg
     1321 atcgcttcta cagcgccatg gcgagctgct gcccgcccct cggcttcgct ggctgcaagt
     1381 ggcagagcgg cgacccggcg aatccgtctc ggggaatgcc ctacgtccag ggcctgttcg
     1441 tcttgagcga gcacgagacg gggcggatgg tggcggtgat cgacgccggg tggatcaccg
     1501 gcaaacggac ggcggcggcc tcggcgctgg tcgcccgcta ccaggctcgc gagggggccg
     1561 aggagctcgc tctcctgggc tgcggcctac aggggcgcgc tcatctgaga gctctcgcgg
     1621 cggagctccc cggcctctcg aggtgccgcg tcttcgacgt cgtccccgag gtccagacgg
     1681 cgttcgtcga ggacctcgac gggcgctgca acggcttgcg cgtacgcggg gcggccgacg
     1741 cggagtccgc ggttcgggga gcggacgtcg tcattccggg aggaccgatc cgggtccggc
     1801 gccagccgac gatcgtctcc gactgggtcg agcccggggc gctcgtcgtc acgatcgact
     1861 acgactccta cgtcactgac gagtgcatcg cggcgatgga cctggtcctg accgacgatc
     1921 gcggccagat cgagagcgcc cggcgcacgg agggcaagtt cctcggtgtg gatcggatcg
     1981 acgccgagat cgcggagatg gtcgccacgg gaagagggcg caggcagacc gacgagcagc
     2041 gcatcctgtc gttcaatctg ggcatcgcgc tggaggacgt ggcggtcgcc gcggaggtct
     2101 atcgccgcgc gcgcgagggc ggcgccggaa tcccgttgcg gccctgacct acccgatgcg
     2161 cccgcgcagt gccagctccg tcgcgacccg gacgaggtga gcatgggcgg caatctccgg
     2221 ttcgccgcgt tcatcgtcgt ggcgtttgtc gtcttcgtgt tcgtcctgcg gctggcgctg
     2281 cgggcgcgta gccgccgccc tccggccggc cgggtgtacg gcacggcgct cgtcgtggtg
     2341 gtcggcggca tgctgttcgc gcgcctgggg gcgcaggccg gacaaccctg gtggatctac
     2401 tacacgctac cggcgctcgt gaccatcctt gtgcctccgg tcgtgttccg catggaccga
     2461 cgcgagaccc tggtgtacct ggtgctcgcc ttcacctcgt cgccgctgat ccacgtcgtc
     2521 ttctcgttct tcctcggatg gaaggagtac atgccgtttc tggaggtccc ggcgatctgg
     2581 gagctctgag gcctgggacg agtggtggac cgacgagcga tccggcaagc acccgaagcc
     2641 ccgagccggt cggctcgagc cctcatcccg aggcttcgct ttcgaccgag tcgctggctg
     2701 tccggcttgc ggacccgccg aaggatcggg gcgcctggcg cgtctaatct catgacagcg
     2761 ctccgggtcc ggcacttgac acacaggcag cgggaggccg gcatgaactt gaagctgaac
     2821 ggagcgtttc gcggcgaagt caaccactcg gcctgggtcg accagatccg cagcatcccc
     2881 tcgcgactct gcccgagcga ggagggtctg gcgctcttcg ccgcgcagcc gctcgccgtc
     2941 gagggcagca gctacgctca cgtcgtcaag ggctgtgacc gctgcagaga gcgcctgcag
     3001 cagctcttgc tcagcagctc ctagggcccg gcggccaccg ttcgggctcg ccaccgagac
     3061 cggtcgctga gtcgccgtgg gtgccggccg cgccccgcgt ccaaggagac gcgaaccgtc
     3121 ggtctcgtcg tgcctacgga ggtggcgact gccagagcac ctggacgatc ttccactcgc
     3181 cgtcgtactt ggccaggtgc atgtagtcga tgccccagaa ggcggtcagc ttgacgctgg
     3241 cggtctggtc gagcacctcg aagatctcga ccgagtgggt ggcgtcgtcg ggcacgtgac
     3301 cctccttctt catgttcgcc gccagctcga cgagagcctc gaaggtcatg gggtagatct
     3361 cgtattcgga cccgggcgcc tcgcggtaga agccgaactt ggcgagctcc gggtgcacgc
     3421 cgcgctcgat gagctcggga cgaccctgct cgaccgcctc gacgtagtcg agcacggcgc
     3481 gctcgacggc ctcgtggtca ctctgggcgg acgccgccgc cgcgaggacg agcgcgagga
     3541 cgacccagga cgctctctgt atgtgtcgca tcgcgccgcg gattctatac cctcggcgat
     3601 gcctccgctg gaggtggggg acgggcgccc gtcggcagtg ccgaagccgg aaacgtcgac
     3661 ggcggatgtc ggtcgactgg aagcgtgaca ggtgcccgtc cccgtctagt gcacctcgta
     3721 gctcagcagc aggcgcaggt tgaccagctc g
//
LOCUS       DBNGNK010000399         2588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1430846,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000399 DBNGNK010000000
VERSION     DBNGNK010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1430846"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="AC150V_11820"
     CDS             <1..245
                     /locus_tag="AC150V_11820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763648.1"
                     /translation="GQTLASLGRIVAASVLAAAAGLLGWWIIDDLLGRGLGAQILSVG
                     GGLAAAAAVYLGAARLLGVRELRALLTLLRGRSDAR"
     gene            249..2036
                     /gene="lepA"
                     /locus_tag="AC150V_11825"
     CDS             249..2036
                     /gene="lepA"
                     /locus_tag="AC150V_11825"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR3763649.1"
                     /translation="MDQSRIRNFSIIAHIDHGKSTLADRILQLTDAVSEREMRDQLLD
                     SMDLERERGITIKAQAVRVAWKGHELNLIDTPGHVDFTYEVSRSLQACEGALLVIDAA
                     QGVEAQTVANAYLAIENDLEIIPVVNKIDLPQADADAAAAEVCELIGGAPEDVLRISA
                     KTGAGVEAVLDAVVERIPPPAGESEAAPRALIFDSSYDQYRGVLAFVRVVDGNMGSRD
                     TLRAMALGTKFEVEELGFMSPAMTAVEALTAGEVGYVVTGLKDVSRLRVGDTLTASKG
                     GAEHALPGYKDVKPMVFAGLFPTDSDDYPELREALERLKLNDAALFYEPETSQALGFG
                     FRCGFLGLLHMEIVRERLEREFSLDLLVTAPSVAYRVQTATGDLVEVHNPADMPREVE
                     SVEEPYIDASVIVPKQYVGDVMQLCTERRGRFDHMEYLSEERVMLSYELPLGEIVLDY
                     YDQLKSRTRGYASFDYDLAGFQEGQLSRVDILVGGEQIDALSLIVHRDAAYERGRQLV
                     DRLKEEIPRQMFDVPIQAAIGSRVIARETVKAKRKDVLAKCYGGDITRKRKLLERQKK
                     GKKRMKQVGGVEVPQEAFLAVLNLDGQKR"
     gene            2033..>2588
                     /locus_tag="AC150V_11830"
     CDS             2033..>2588
                     /locus_tag="AC150V_11830"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3763650.1"
                     /translation="MSERALVEHLYLHLPFCAHRCGYCDFVTAVGRRDEHGGYVDALL
                     TELDLERELLAESPATIFLGGGTPTFTEPAALARLLAALPRSAELTVEANPETVTPGL
                     AALLAREGVSRVSLGAQSFQPHLLRILERRAGPDDVRRAVHILRDAGFDNISLDLLYG
                     VPGQSSADLDRDLTEALALAPEHLS"
BASE COUNT          416 a          872 c          880 g          420 t
ORIGIN      
        1 tcggccagac cctcgcctca ctcggccgca tcgtcgccgc gtcggtgctg gcggcggcgg
       61 cgggtctgct ggggtggtgg atcatcgacg atctactcgg tcgcggactc ggcgcgcaga
      121 tcctctcggt cggcggtggg ctcgccgccg ccgccgccgt gtatctgggc gcggcccgtc
      181 tcctcggcgt gcgcgagctg cgcgcgttgc taacgttgct gcgcgggcgc tccgatgccc
      241 gttgaggcat ggaccagagc cgcatccgca acttctcgat catcgcgcac atcgaccacg
      301 gcaagtcgac gctcgccgat cgcatcctgc agctgaccga cgccgtcagc gagcgggaga
      361 tgcgcgatca gctgctcgac tccatggatc tcgagcgcga gcgcgggatc acgatcaagg
      421 cccaagccgt ccgggtggcg tggaagggcc acgagctgaa cctgatcgat acgcccggac
      481 acgtggattt cacctacgag gtgagtcgat ctctacaggc ctgtgagggc gcgctgctcg
      541 tcatcgacgc cgcccagggc gtcgaggctc agaccgtcgc caacgcctac ctcgcgatcg
      601 agaacgacct cgagatcatt ccggtcgtga acaagatcga cctgccgcag gctgatgcgg
      661 acgccgctgc agccgaggtc tgcgagctga tcggcggcgc tccggaggac gtgcttcgca
      721 tctccgccaa gacgggtgcc ggcgtggagg ccgtgctcga cgccgtcgtc gaacggattc
      781 caccacctgc cggggagtcc gaagcggcgc cgcgggcgct gatcttcgac tcgtcgtatg
      841 accagtaccg cggcgttctc gccttcgtgc gcgtggtcga cgggaacatg ggctcacgcg
      901 acacgctccg tgcgatggcg ctcggcacga agttcgaggt ggaggagctc ggcttcatgt
      961 cgccggccat gactgcggtc gaggcgctca cggccggcga ggtggggtac gtcgtcacgg
     1021 ggctgaagga cgtctcgcgg ctccgcgtag gggacacgct caccgcgagc aagggaggag
     1081 ccgagcacgc gcttcccggc tacaaggacg tcaagccgat ggtgttcgcc gggctctttc
     1141 ccaccgactc ggacgactac cccgagctgc gcgaggccct ggagcggctg aagctcaacg
     1201 acgcggcgct cttctacgag cccgagacgt cgcaggcgct cggcttcggc ttccgctgcg
     1261 gcttcctcgg gctcctgcac atggagatcg tgcgcgagcg gctcgagcgc gagttcagtc
     1321 tcgatctcct cgtgacggcg cccagcgttg cctatcgagt gcagacggca actggagact
     1381 tggtcgaagt gcacaacccg gccgacatgc cgcgcgaggt ggagagcgtg gaggagccgt
     1441 acatcgacgc atcggtcatc gtcccgaagc agtacgtcgg cgacgtgatg cagctctgca
     1501 ccgagcggcg cggacgcttc gaccacatgg agtacctctc ggaggaacgc gtgatgctct
     1561 cgtacgagct cccactcggc gagatcgtgc tcgactatta cgaccagctg aagtcgcgga
     1621 cgcgcgggta cgccagcttc gactacgacc tcgccggttt ccaggagggc cagctcagcc
     1681 gggtcgacat tctcgtcggc ggcgagcaga tcgacgcgct ctcgctgatc gtgcatcgag
     1741 acgcggccta cgagcgaggc cggcagctcg tcgatcgcct gaaggaggag ataccgcgcc
     1801 agatgttcga cgtgccgatt caggcggcta tcggctcgcg ggtgatcgcg cgcgaaaccg
     1861 tcaaggcgaa gcgcaaggac gtgctcgcga agtgctacgg cggcgacatc acgcgcaagc
     1921 gcaagttgct ggagcggcag aagaagggga agaagcggat gaagcaggtc ggaggcgtcg
     1981 aggttccgca agaagccttc ctcgcggtac tcaacctcga cgggcagaag cgatgagcga
     2041 gcgtgccctc gtggagcacc tctacctcca cctcccgttc tgcgctcatc gctgcggcta
     2101 ctgcgacttc gtgacggctg tcggtcgccg cgacgaacac ggcgggtacg tcgacgccct
     2161 cctgacagag ctcgacctcg agcgagagct gctcgccgag tcccccgcca ccatctttct
     2221 cggcgggggc acgccgacct tcacggagcc ggccgcgctc gcccggctgc tggcggcgct
     2281 gccgcgctcc gccgagttga cggtcgaagc caatcccgag accgtgaccc ccgggctggc
     2341 tgcattgctc gcgcgcgagg gtgtctcgag ggtctcgctc ggagcgcaga gcttccagcc
     2401 gcacctgctt cggatactcg agcgacgagc aggcccggac gacgtccggc gggccgtgca
     2461 cattttgcgc gatgccggtt ttgacaacat ctcgctcgac cttctctacg gcgtgcccgg
     2521 ccagagctcc gccgacctcg atcgcgacct gaccgaggcg ctcgcgctcg cgcccgagca
     2581 cctctcgt
//
LOCUS       DBNGNK010000400         3921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_858455,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000400 DBNGNK010000000
VERSION     DBNGNK010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_858455"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1073
                     /locus_tag="AC150V_11835"
     CDS             186..1073
                     /locus_tag="AC150V_11835"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR3763651.1"
                     /translation="MKTMRILSIGWLAGCAALWAGESVDRTIPVRGDVEVRIENIAGS
                     VEVEVWGQSEVRVVAELGDDVEGLDIDADDDEVRIEVEIPDRSGRRRNRDIDSDVKVW
                     VPADASVEVETVSASIDVRDVGGALDLESVSGSIEASATSDNADLATVSGNIEFEGSA
                     TAVDAETVSGRVRLSGVSRNVDVSTVSGSVKVAASAIERADFESVSGSIEFRADLAVG
                     ARVDFSSHSGNIELFLPAGTAASFEIETFAGDIRSDFGGEARRTSRYAPGRELYHSTG
                     SDAKISVETFSGNVYLGKD"
     gene            1213..2712
                     /locus_tag="AC150V_11840"
     CDS             1213..2712
                     /locus_tag="AC150V_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR3763652.1"
                     /translation="MPRPPVPVNELHEIELLVRSFQPFLVLETVELDRADVLLEHLAD
                     GLGLPYFRWTRATGLRRQGERGPIYGTEDLAVALDHVAASKLEALYDFRDAEEAFEER
                     ALLSKLRELHAPLCGHRGALVFTGSGFVIPESFAPLINVVRLSPPSDEEYRRYLSALL
                     EEVRERIPVTVDLDSGQVAELIGHLRGLTLMEVRKIVTKVLVVDGRLGAGDLDRVVRE
                     KRDIVERTGVLEYFPAEEGLEAIAGLQALKGWLASRAAAFRDSRRASAYGLTPPRGVL
                     LAGVQGCGKSLSAKAVARAWGLPLIRLDPARLYNKFVGESEKNLRLAIETAERLAPIV
                     LWIDEIEKALAPASEGDGGVSRRLFGTFLTWLQEKRQPVFVVATANDVSRLPPELTRK
                     GRFDEIFFVDLPGAEDRRGILSLHLARRGRDPSGFDLERLVEATEGFSGADIEQAIVS
                     SLYAAFHAGAELSTDRLGEEIARTRPISVTRAESIAALRDWAEGRCVPA"
     gene            complement(2815..>3921)
                     /locus_tag="AC150V_11845"
     CDS             complement(2815..>3921)
                     /locus_tag="AC150V_11845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009023011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR3763653.1"
                     /translation="WPDLGFGYTGQTGYYIFTDRGGQRIERAVLGIGGPLLERSGAYD
                     YFGSADELADFVRARDPRVIGLNISRQIPGADGLSHSGYLEIVGVLGEPFSERIVSAE
                     KLVSDFRSRRVASEIAAFAEAGEISRQIAERALSNEVISPGVTTLEEVAWWIRDRLFE
                     RGLDLSFGMPSIYITGPEGIEAISDERIIQRGDLMMIDWGVGLMNFYTDMKRIAYVLR
                     EGETEAPAGLQHAFQRGLEARAVVRRAIRAGITAKQAEEEIYQALEAAGFRRIGFNQP
                     TDDPESTDVVIGCHSVGNSGHGIGPSIAFFNPKRITFEIKPSNLLSIELFAYTAAAEW
                     GGAKVRIPLEDDAVVTARGVEWLYPANERILLVR"
BASE COUNT          592 a         1314 c         1398 g          617 t
ORIGIN      
        1 cctggagatc atcgatcgag ccatcggaga gatctatcag gcgctcgagg aggacccgga
       61 cagcctgacg ctcgagctgc tgctggcgtc cacccatcag ctggaggtgg agttcctcaa
      121 acaggtcaat gtgctctagg agtgcgcgaa tcgcgcccgc ggagcttccc taaggagtga
      181 acgtcatgaa gacaatgagg attctgtcga tcggatggct ggcgggctgc gctgccctct
      241 gggcgggtga gtccgtcgac cggacgattc ccgtgcgcgg cgacgtcgag gtccgaatcg
      301 agaacatcgc cggctcggtc gaggtcgagg tctggggcca gtcggaagtc cgcgtggtgg
      361 ccgagctggg cgacgacgtc gagggcctcg acatcgacgc cgacgacgac gaggtccgta
      421 tcgaggttga gattcccgac cgttccgggc gtcgccgcaa ccgagacatc gattccgacg
      481 tcaaggtgtg ggtgccggct gacgcctccg tcgaggtcga gacggtgagc gcctcgatcg
      541 acgtgcggga cgtgggcggc gcgctggacc tggagtcggt cagcggctcg atcgaggcga
      601 gtgcgacgtc cgacaacgcc gatctggcga cggtgagcgg caacatcgag ttcgagggct
      661 cggccaccgc ggtcgatgcc gaaaccgtga gcggccgcgt gcgtctcagc ggcgtgtcga
      721 ggaacgtcga cgtctcgacg gtgagcggca gcgtcaaggt cgcggctagc gccatcgagc
      781 gcgccgattt cgagtcggtg tcgggctcga tcgagttccg ggccgatctc gccgttggtg
      841 cccgcgtcga cttcagcagc cacagtggca acatcgagct cttcctgccg gccgggacgg
      901 cggcgagctt cgagatcgag acctttgccg gcgatatccg ctccgacttc ggtggcgagg
      961 cacgtcggac cagccgctac gcgccgggcc gcgagctcta ccactcgacc ggctcggacg
     1021 ccaagatctc ggtcgagacc ttcagcggca acgtctatct gggcaaggac tgacgcggag
     1081 ctgcccggca gacctgcggg gccggtgcct cctggcgccg gccctccctg cgggcgtcgg
     1141 ccgcggcgtg gctccggagc gccggtccgc cggcgagcga cgggcggtaa cggctacaat
     1201 ccgcccgacg acatgccgcg cccgcccgtt ccggtcaacg agctgcacga gatcgagctg
     1261 ctcgtgcgct cgtttcagcc gttcctcgtc ctggagacgg tggagctcga tcgagccgac
     1321 gtgctgctcg agcatctcgc cgacgggctc gggctgccgt actttcgctg gacccgcgcc
     1381 accggactgc gccgccaggg cgagcgcggc ccgatctatg gcaccgaaga tctcgcggtg
     1441 gccctcgatc acgtggcggc ctcgaagctc gaggcgctct acgactttcg cgacgcggag
     1501 gaggccttcg aggagcgggc cctgctctcc aagctgcgtg agctccacgc accgctctgc
     1561 ggccaccggg gcgcgctggt gttcaccggt agcggtttcg tgattcctga atcgttcgcg
     1621 ccgctcatca acgtcgtgcg gctgtcgcca ccgagcgacg aagagtatcg gcggtacctc
     1681 tcggctctgc tcgaggaggt ccgcgagcgc attcccgtca ccgtcgacct cgacagcggg
     1741 caggtcgcgg agctcatcgg ccacctgcgc ggcctgaccc tgatggaggt gcgcaagatc
     1801 gtcaccaagg tcctggtggt cgacggccgg ctgggcgccg gcgatctcga tcgcgtcgtt
     1861 cgcgagaagc gcgacatcgt cgagcgcacc ggggtgctcg agtacttccc cgccgaagag
     1921 ggtctcgaag cgatcgccgg gctccaggcg ctcaagggct ggctcgcgtc gcgagccgcg
     1981 gccttccggg acagccgacg ggcgagcgcc tacgggctga ctccgccgcg gggcgtgctg
     2041 ctcgccggtg tccaggggtg cggcaagagc ctgagcgcca aggccgtggc gcgagcctgg
     2101 ggcctgccgc tgatccggct ggatccggcg cggctctaca acaagttcgt cggcgagtcg
     2161 gagaagaacc tgcgtctcgc catcgagacg gcggagcggc tggcgccgat cgttctctgg
     2221 atcgacgaga tcgagaaggc gctggcgccg gcgagcgagg gcgacggtgg cgtctcccgg
     2281 cgtctgttcg gcacctttct gacctggctg caggagaagc ggcagccggt cttcgtggtg
     2341 gcgacggcca acgacgtctc ccgcctgccg cccgagctga cgcgcaaggg ccgtttcgac
     2401 gagatcttct tcgtcgatct tccgggtgcc gaggaccgcc gagggatcct gtcgctgcac
     2461 ctggcgcgcc gcggtcgtga cccgtcgggg ttcgatctcg agcggctcgt cgaggcgacc
     2521 gaaggcttca gcggcgccga catcgagcag gcgatcgtct cgtcgctcta cgccgccttc
     2581 catgccggcg ccgagctcag caccgaccgg ctcggcgagg agatcgcgcg cacccgtccg
     2641 atctcggtca cccgcgccga gtcgatcgcc gcgctccgcg actgggccga gggacgctgc
     2701 gtcccggcct gacgggtctg ccgtccgata gggcacaacc cctggagaag ccgggctcga
     2761 ctcggcccgg acggccgtgc cgcctgcaac caggggcgga ggaaagctgc gcccctagcg
     2821 gaccagcagg atgcgctcgt tcgccgggta gagccactcc actccccgcg ccgtcacgac
     2881 ggcgtcgtcc tcgagcggga tgcggacctt ggcgccgccc cactcggcgg cggccgtata
     2941 ggcgaagagc tcgatcgaca ggaggttcga gggcttgatc tcgaaggtga tgcgcttggg
     3001 gttgaagaag gcgatcgagg ggccgatgcc gtgccccgag ttgcccaccg agtggcagcc
     3061 gatgacgacg tcggtcgact ccgggtcgtc ggtcggctgg ttgaagccga tgcggcggaa
     3121 tccggccgcc tcgagcgcct ggtagatctc ctcctcggcc tgcttggccg tgatcccggc
     3181 gcggatcgcg cggcgcacga ccgcgcgcgc ctcgaggccc ctctggaagg cgtgctggag
     3241 cccggccgga gcttcggtct cgccctcccg caggacgtag gcgatgcgct tcatgtcggt
     3301 gtagaagttc atcaggccga cgccccagtc gatcatcatg aggtcgccgc gctggatgat
     3361 gcgctcgtcg gagatggctt cgatgccctc gggtccggtg atgtagatcg acggcatgcc
     3421 gaaggacaga tccagcccgc gctcgaagag acggtcgcgg atccaccagg cgacctcctc
     3481 gagcgtcgtc acgccgggcg agatgacctc gttcgagagc gcccgctcgg cgatctgacg
     3541 cgagatctcc ccggcctcgg cgaaagcggc gatctcggat gcgacccgtc gcgagcggaa
     3601 gtccgacacc agcttctcgg ccgagacgat gcgctccgag aaaggctcgc cgagcacccc
     3661 gacgatctcg agatagccgc tgtgagacag cccgtcggcg ccgggaatct ggcgcgagat
     3721 gttgagcccg atgacccgag ggtcgcgcgc gcgcacgaag tccgccagct cgtcggccga
     3781 gccgaagtag tcgtaggcgc cgctcctctc gagcagtggc ccgccgatgc ccagcaccgc
     3841 ccgctcgatg cgctggccgc cccggtcggt gaagatgtag taaccggtct gccccgtgta
     3901 gccgaagccg agatcgggcc a
//
LOCUS       DBNGNK010000401         8947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_713903,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000401 DBNGNK010000000
VERSION     DBNGNK010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_713903"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1367)
                     /gene="miaB"
                     /locus_tag="AC150V_11850"
     CDS             complement(45..1367)
                     /gene="miaB"
                     /locus_tag="AC150V_11850"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR3763654.1"
                     /translation="MKRYHVTTFGCQMNAHDSERIKGMLESIGLGEAVKPEAADVLVF
                     NTCTIREKPDRKLASYLGQAAARKRENPEVVVAVGGCYAEAQREQIFERYPFVDVAFG
                     PGSIPHLAEWIGAGGVGVSRGRFGAHEHFAAELPASRERRFQAWVQVSMGCNSVCSYC
                     IVPAVRGREQSRRPGDVVAEVTRLAGEGVREITLLGQNVNSWGRDLAPEIKTDFSELL
                     RACDAVEGIDRIRFTSPHPKDFRPPLIKALAECASVCEHVHLPLQSGSSRLLKAMRRT
                     YDSERYLRLVEALRAAVPDLALGTDLIVGFPGETEEDFFQTVKLVEEVGFDSAFTFIF
                     SPRSGTEAAELPQQVSYETKRERLERLVEVVQRIAATRNAERIGGVEEVLVEGASRTD
                     PALLRGRTRRNTTVNFTGDAAPGELVDVLIESSTSTTLRGRAHVYAAA"
     gene            complement(1507..3039)
                     /gene="rny"
                     /locus_tag="AC150V_11855"
     CDS             complement(1507..3039)
                     /gene="rny"
                     /locus_tag="AC150V_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR3763655.1"
                     /translation="MLTVVALIVGGVVGAGLAFLWLRTATGSRLRRAQDERRQLMDEA
                     EREADALRREAQIEAREQAVKLRTEIEQEVGDRRLEIAKIEERISQTQAEADAKLTEL
                     ARREQGMADRETHLKQMQEELKVARDDAVGELQRISAMTVGEAKAQLLDRALDLIRHE
                     LARDVRQMEEEARADARRRARALVADSLQRVAASHTAETTVTVVELPTDDMKGRIIGR
                     EGRNIRALEHLTGVDFIIDDTPHAVVLSSFDGIRREIARMTVEKLIEDGRIHPARIEE
                     TYYASKAELDEYVLQAGQQAVFEANCGEFHEELIKVLGRLRYRTSYGQNVLKHTLEVV
                     HLAGIMASELGASVKTAKRAALLHDLGKAMTHEVEGSHAEISAQLARRYGESQGVVHA
                     IEAHHYEEQPQTVEAVLLIAADAISASRPGARGESLEHYIRRLSQLEEIAAAKPGVAK
                     AYALQAGREIRVMVEPTEVDDDGAVLLSHEIAREIEDQLEYPGQVKVIVIRESRAVEV
                     AK"
     gene            complement(3280..3876)
                     /locus_tag="AC150V_11860"
     CDS             complement(3280..3876)
                     /locus_tag="AC150V_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763656.1"
                     /translation="MTVPTVTALREHGSRRVAVELGGRPWRVLPVEVVARTGLRVGLQ
                     LEREHLRLLRHELRAAEVDTAAARLLRHREHTTESLDERLAGRGFDRARRAEAVARLE
                     AAGLVDNRRFAFERARLLSDRDAGDRMIEADLAAHGVSSALRREAIAALDPEADRATK
                     IVARRGAGAKTLRRLASRGFGEDSLEAHIAASWERAVP"
     gene            complement(3916..5004)
                     /gene="recA"
                     /locus_tag="AC150V_11865"
     CDS             complement(3916..5004)
                     /gene="recA"
                     /locus_tag="AC150V_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004945402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR3763657.1"
                     /translation="MNREEALDVALGQIERQFGKGSVMRMSDQAQVAIAAISTGSLSL
                     DLALGIGGLPRGRVVEVFGPESSGKTTLVYHVMAEAQRRGGICAFVDAEHSMDPSYAR
                     RIGVNIDELLVSQPDTGEQALEIAELLIRSGALDVVAVDSVAALTPKAEIEGEMGDSH
                     VGLQARLMSQALRKLTANLNKTKTICVFINQLREKIGVMFGSPETTPGGRALKFYSSV
                     RLDIRRIETLKDGAEAFGNRVRVKVVKNKVAPPFKQAEFDIIYGSGISWEGSVLDVAL
                     ERKVVQKSGSYFSYGDERLGQGRQNATAFLGEHPDIVQAILAHIQAEVPSEQVVSARL
                     LPVLPDSAEGIEAVPAEAEAEAEAAAKG"
     gene            complement(5039..5581)
                     /locus_tag="AC150V_11870"
     CDS             complement(5039..5581)
                     /locus_tag="AC150V_11870"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: protein motif:HMM:NF024952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HIR3763658.1"
                     /translation="MADDDTLRLFLALRLPSETIDELVRWGDAALPAARRVPREHLHV
                     TLAFLGARLADELPSIVGALRAAAAAAAPFLLEPLRYRETRSVGMVVLDDPSGEAALL
                     AADLHGRLERLHVYRPEKRRWLPHVTVLRFRERPRLDPPPPALGRFAPSDAAAFLSRL
                     HPSGARYELLETAQLGSRST"
     gene            complement(5596..6867)
                     /locus_tag="AC150V_11875"
     CDS             complement(5596..6867)
                     /locus_tag="AC150V_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HIR3763659.1"
                     /translation="MTRPRAAIVVTGSELVRGERSDRNGPFFAGEALRIGLEPWRITI
                     VGDAPADLLAALEEGLGADVMLVSGGLGPTHDDRTVELVAAATGRGLVVDSGLEAEIE
                     GVSRMVADRLGRPYADFAHGVRKQATLPDGAISLGLAGTAPGLVVDAGTCVIVVLPGP
                     PRELQRLWPAALETDAVRRVLARTAPPGRRVLRFFGASESAVAKALAEAGGDGDGVEV
                     TICARDFEIHVDLLVQPGADKRADELAKALAAPLERYLFTQDERSIEEIVLELCRARG
                     LTLATAESCTGGLVAARLTGVPGSSDVFRGSVVAYADEVKEHELGVSQELIARHGAVS
                     AEVAAAMAAGVRQRLAADVGVAVTGVAGPGGGTPEKPVGLVHLHASGPNGEKEHRFEF
                     PGDRDWIRTRTSVSALHLVRRLLTQSRPERV"
     gene            complement(6864..7574)
                     /locus_tag="AC150V_11880"
     CDS             complement(6864..7574)
                     /locus_tag="AC150V_11880"
                     /inference="COORDINATES: protein motif:HMM:NF024855.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HIR3763660.1"
                     /translation="MFEIGSSLREARVRQGLDLEELEQRTKIRRKYLRALEEEQFEQL
                     PGHTYVRGFLRTYADALDLDGQLYADEYSTRFVGGEDDAAPLDRAPTSRVGRQRERRE
                     SRVLVLALVVIGVATALVFVAWKFGGPETERIPGLAQAGAGSGSSNAAPLAKLEVRAP
                     RGDSFMEVRAGSRAGKPLYRGTIQQGQVQRFERRSLYVRLGSPANLALKLNGNRIELP
                     SGKTFVVSPAGVQTAASS"
     gene            complement(7612..7887)
                     /locus_tag="AC150V_11885"
     CDS             complement(7612..7887)
                     /locus_tag="AC150V_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763661.1"
                     /translation="MPAAVLATRYSETYELLHAGFAIPLGFALGLVAVLAARSVRRLD
                     ERSVGPTRGLGAARLGRILGVVGMCLAGTAAISVGVYGLLEWAGSRG"
     gene            complement(7955..8941)
                     /locus_tag="AC150V_11890"
     CDS             complement(7955..8941)
                     /locus_tag="AC150V_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763662.1"
                     /translation="MIGMAIGYLATVSSVGKLEAVVVWGALFASMLRFATPLVFASLG
                     GLFSERSGVVNIGLEGMMLMGAFFAVWGSDVTGSWVLGLLIGMLAGGIAAFVHAFFSV
                     TLRSDQIVGGTAINFLALGVTGYLFVDIYGTAGTPTDLSTIPDVSLGFWDSIPGIGNF
                     LDDVLGQLNLMIWIAIIMVFVVYVVVFKTPIGLRIRSVGEHPKAADTVGINVYATRYA
                     AVTMSGVLAACGGAYISLGFVDGFTENMIAGRGFIALAALIFGNWRPFGLAAACLLFG
                     FSSALARALQEYSTAVGTLFEALPYVLTLIAVAGVIGRSIPPAAIGRPYVKS"
BASE COUNT         1423 a         3178 c         2932 g         1414 t
ORIGIN      
        1 cgttcaggca tccctcccag acgagctcgc gcgcgctcac gcgcctacgc cgccgcgtag
       61 acgtgcgcgc ggccgcggag cgtcgtcgac gtcgagcttt cgatcaggac gtccaccaac
      121 tccccggggg cggcatcgcc cgtaaagttc accgtcgtgt tccggcgcgt gcgtccgcgc
      181 aacagtgctg gatccgtgcg gctcgcgccc tcgaccagca cttcttcgac tccgccgatc
      241 cgctctgcat tgcgcgtagc ggcgatcctc tgcacgacct cgacgagacg ctcgagccgt
      301 tcacgcttcg tctcgtacga gacctgctgc ggcagctcgg cggcctccgt cccactccgt
      361 ggcgagaaga tgaaggtgaa ggcgctgtcg aagccgactt cctctacgag cttcaccgtc
      421 tggaagaagt cttcctccgt ctcgccggga aagccgacga tcaggtcggt cccgagcgcg
      481 agatccggca cggccgcacg cagcgcctcg acgagcctca ggtagcgctc gctgtcgtag
      541 gtgcgtctca tcgccttcag gaggcgcgac gagccggatt gcaggggcag atggacgtgc
      601 tcgcagacgc tcgcacattc cgccagcgcc ttgatcagcg gcggacggaa gtccttcggg
      661 tgcggactcg tgaagcgaat gcggtcgatt ccttccaccg catcgcaggc ccgcagcaac
      721 tcgctgaagt ccgtcttgat ctccggagcc agatctcgtc cccatgagtt gacgttctgt
      781 cccagcagag tgatctctcg aacaccctcc ccggccagtc gtgtcacctc cgcgacgacg
      841 tcaccggggc ggcggctctg ctcccgtccg cgcaccgccg gcacgatgca gtacgagcac
      901 accgagttgc accccatcga cacctgcacc cacgcctgaa agcggcgctc gcgcgacgcg
      961 ggcagctcag cggcgaagtg ctcgtgcgct ccgaagcgac ctctgctgac ccccacgccg
     1021 cctgcgccga tccactcggc cagatgcggg atcgaccccg gtccgaaagc gacgtcgacg
     1081 aaggggtagc gctcgaagat ctgctcccgc tgtgcctccg cgtagcagcc gccgaccgcg
     1141 acgaccactt ccgggttctc acgcttccgc gccgccgcct gcccgaggta cgaggccagc
     1201 ttccggtccg gcttctctct gatcgtgcag gtgttgaaca cgagaacgtc ggccgcttcc
     1261 ggcttcaccg cctccccgag cccgattgac tcgagcatcc ccttgattcg ctcggagtcg
     1321 tgggcgttca tctggcaccc gaacgtcgtg acgtgatacc gcttcacggc aggccagtgt
     1381 agccgggagc ctcgccggcc tctggcgctc aggcgtgccg gctgttcgcc cagatcgtcc
     1441 agccgaggat cggcccgaac gggaccacgc cgacggcgac ggcgagcacg gcagtctcga
     1501 acgtgctcat ttcgctacct ccacggcgcg ggactcgcga atcacgatca ctttgacctg
     1561 acccgggtac tccagctgat cctcgatctc cctcgcgatc tcgtgcgaca gcaggacggc
     1621 gccgtcgtcg tccacctctg tgggttccac catcacccgg atctcccggc ccgcttgcag
     1681 cgcgtaggcc ttcgcgacgc ccggcttggc cgcggcgatc tcctcgagct gcgacagccg
     1741 ccggatgtag tgctcgaggc tttcgccgcg tgcgcccggg cgcgatgctg aaatcgcgtc
     1801 cgcggcgatc agaagcaccg cttcgacagt ctgcggctgc tcttcgtagt ggtgcgcctc
     1861 gatcgcgtgc acgactccct gcgactcgcc gtagcgtcga gcgagctgcg ccgagatctc
     1921 ggcgtgtgag ccctccacct cgtgcgtcat cgccttgccg agatcgtgca gcagcgcggc
     1981 ccgcttggcg gtcttcaccg aggcgccgag ctccgacgcc atgattcccg ccagatggac
     2041 gacctcgagc gtgtgcttga ggacgttctg accgtagctc gttcggtagc ggagccggcc
     2101 gagcaccttg atgagctctt cgtggaactc accacagttg gcttcgaaca ccgcctgctg
     2161 gccggcctgc aggacatatt cgtcgagctc agccttcgag gcatagtagg tctcctcgat
     2221 ccgggccggg tgaatccggc cgtcctcgat cagcttctcg accgtcatgc gggcgatctc
     2281 gcggcggatt ccgtcgaacg acgagaggac gaccgcgtgc ggcgtgtcgt cgatgatgaa
     2341 gtcgacaccc gtgaggtgtt cgagcgcccg tatgttgcga ccctcgcgcc cgatgatgcg
     2401 ccccttcata tcgtcggtcg gcagctcgac gaccgtcacc gtcgtctcgg ccgtgtgact
     2461 agcggccacg cgctggagcg agtcggcgac cagcgcgcga gcgcggcgcc gtgcgtcggc
     2521 tcgagcttcc tcctccatct gcctcacgtc acgcgcgagc tcgtgcctga tcaggtcgag
     2581 ggcgcgatcg agaagctgtg ccttggcctc cccgaccgtc atcgccgaga tccgctgcag
     2641 ctcgccgacc gcatcgtcgc gggccacttt gagctcttcc tgcatctgct tcaggtgcgt
     2701 ctcgcggtcg gccatgccct gctctcggcg cgcgagctcg gtcaacttcg cgtcggcctc
     2761 cgcctgggtc tggctgatcc gctcctcgat cttcgcgatc tcaagccgcc ggtcaccgac
     2821 ttcctgctcg atctcggtgc gcagcttgac ggcttgctcg cgtgcctcga tctgggcctc
     2881 gcgtctcagc gcatccgctt cgcgctccgc ttcgtccatc agctgccggc gctcgtcctg
     2941 ggcccttcgc agcctggagc cggtggctgt gcggagccag aggaaggcga gtcccgcgcc
     3001 gacgacgcca ccgactatca gcgcgactac cgtgagcatt ccggctcctc gagacgaaga
     3061 ctcgattgcc gcggcttcgc cacggctgct cacacccggt cacttcgcct caagaggcga
     3121 atccggaacc ctcggtgcta cgtcagtgcg tcggccctgt cggagccctc cggggcggcc
     3181 ggcggaatcg tgatagtcga cgctgcgtag agattgaagt cgctgacatg accgacgttt
     3241 cagaactgct tcgagtggtg aaatgtgggt gaagcatctc tatggtaccg ctctctccca
     3301 cgacgctgca atgtgcgcct cgagcgagtc ctcgccgaag ccgcgcgaag ccagacgccg
     3361 cagggtcttg gcaccggcgc cgcgcctcgc gacgatcttg gtagcgcgat cagcctccgg
     3421 gtcgagggct gcgatcgcct cgcgtcggag cgcgctgctc acgccatgcg cggccagatc
     3481 ggcctcgatc atgcgatcgc cggcgtcgcg gtcggagagc agccgcgccc gctcgaaggc
     3541 aaaacgccgg ttgtcgacca gccccgcggc ctcgaggcgg gcgactgcct ccgcgcgccg
     3601 cgcccggtcg aaccctcgcc cggccaaacg ctcgtcgagt gactccgtcg tgtgctctcg
     3661 atgtcgcagc aacctggcgg cagctgtatc gacctccgct gcccgcagct catgccggag
     3721 tagacgaagg tgttcgcgct cgagctgcag cccgacgcgg agtcccgtgc gagcgacaac
     3781 ctcgaccggg agcacccgcc acgggcggcc gccgagctcg accgcgacac gccgtgatcc
     3841 gtgctcgcga agcgccgtca cggtcgggac cgtcactgcg caacccgcag cttggcagac
     3901 atccctcgac ggacgttatc ccttcgccgc cgcttccgct tccgcttccg cctccgccgg
     3961 aacagcttcg atcccctcgg cggagtcggg cagcaccggc agcaggcgcg ccgacacgac
     4021 ttgctcggat ggcacctccg cctgaatgtg cgcgaggatg gcctgcacga tgtcggggtg
     4081 ctcgccgagg aacgcggtgg cgttctgccg cccctggccg agccgttcgt cgccgtagct
     4141 gaagtaggag cccgacttct gcacgacctt ccgctcgagg gcgacgtcca agacactgcc
     4201 ctcccaggag atgcccgagc cgtagatgat gtcgaactcg gcctgcttga aggggggagc
     4261 caccttgttc ttcaccacct tgacgcggac gcggtttccg aacgcctccg cgccgtcctt
     4321 gagcgtctcg atgcggcgga tgtccagtcg gacggacgag tagaacttga gcgcacggcc
     4381 gccaggcgtc gtctcggggg agccgaacat cacgccgatc ttctcgcgca gctggttgat
     4441 gaagacacag atggtcttgg tcttgttgag gttggctgtc agcttgcgca gcgcctggct
     4501 catcaggcga gcctgcagac cgacgtggct gtctcccatc tcgccctcga tctccgcctt
     4561 cggcgtgagc gctgcgactg agtcgacggc gacgacgtcg agagccccgg aacggatcag
     4621 gagctcggcg atctcgagcg cctgctcccc cgtgtcgggc tgcgagacga gcagctcgtc
     4681 gatgttgacg ccgattcggc gcgcgtacga agggtccatc gagtgctcgg cgtcgacgaa
     4741 ggcgcaaatg ccgccacgtc gttgtgcctc ggccatgacg tggtagacga gtgtcgtctt
     4801 gcccgacgac tccgggccga agacctcgac gaccctgccc cgtggtagtc caccgattcc
     4861 gagtgcgagg tccaacgaga gcgatccggt cgaaatcgcc gcgatcgcga cctgcgcctg
     4921 gtcgctcatg cgcatcaccg atcccttgcc gaactgtcgc tcgatctggc cgagtgctac
     4981 gtcgagtgct tcctcgcgat tcatctggtg gtcctcctag cgcatctcgt gtcgttcgtc
     5041 acgtcgatcg tgatcccagt tgtgcggtct ccagcagttc atagcgtgcc cccgacgggt
     5101 gcagccgaga cagaaaagca gctgcgtcgg acggcgcgaa ccgtcccagc gcgggtggcg
     5161 gcgggtcgag tcgcgggcgc tcgcggaagc gcagcacggt cacgtgagga agccaccgcc
     5221 gcttctccgg tcggtagacg tggagtcgct ccagccgccc atgcaggtcc gcggcaagca
     5281 gcgcggcctc tcccgaagga tcatcgagca cgaccatgcc gacgctgcga gtctctcggt
     5341 agcgaagcgg ctcgagcaga aacggcgccg cggccgccgc ggcagcgcgc agcgcgccca
     5401 cgatcgacgg cagctcgtcg gccaggcggg caccgaggaa tgcaagcgtg acgtgaaggt
     5461 gctcccgcgg cacacgccga gctgcgggga gcgccgcatc accccaccgc acgagctcgt
     5521 cgatggtctc tgaaggaagc cgaagagcga gaaagagccg cagcgtgtca tcgtccgcca
     5581 cgctagcggg acggctcata cgcgttcggg ccggctttgt gtcaagagac ggcgcaccag
     5641 atggagcgcc gataccgacg tccgcgttcg aatccagtcg cggtcgcccg ggaactcgaa
     5701 ccggtgctcc ttctcgccgt tcgggcccga cgcgtggagg tggacgagcc cgaccggttt
     5761 ctcgggtgtg ccgccgcccg gcccagcgac ccctgtcacg gcgacaccga cgtcggccgc
     5821 caggcgctga cggacgcccg cggccatagc ggccgccacc tcggccgaaa ccgctccgtg
     5881 gcgcgcgatc agctcctgcg aaaccccgag ctcgtgctcc ttcacctcgt ccgcgtacgc
     5941 gacgaccgag ccgcggaaga cgtcgctcga ccccggaacg ccggtcagcc tcgccgccac
     6001 cagacccccg gtgcaggact cggcggtcgc gagggtcagc ccacgtgcgc ggcaaagctc
     6061 gagcacgatt tcctcgatcg accgctcgtc ctgagtgaac aggtaccgct ccagcggcgc
     6121 ggcgagcgcc tttgcgagct cgtcggcgcg cttgtccgct cccggctgga cgagcaggtc
     6181 gacatggatc tcgaaatcgc gggcgcagat cgtgacctcc accccgtcac cgtcgcctcc
     6241 tgcttcggca agcgctttcg caaccgccga ctcgctcgcg ccgaagaagc ggagcacgcg
     6301 gcggcccggc ggtgccgtgc gcgcgagaac gcggcgcacg gcgtcggtct cgagagccgc
     6361 cggccagagt ctctgcagct ctcgcggcgg gcccggcagc acgacgatca cgcaagtccc
     6421 tgcgtcgacg acgaggcccg gagctgtgcc ggccaggccg agcgagatcg ccccatcggg
     6481 cagtgtcgcc tgcttccgga cgccgtgcgc gaagtcggca tatgggcgac ccagccggtc
     6541 ggcgaccatc cgcgaaacgc cttcgatctc ggcctccagc ccgctgtcca cgaccagacc
     6601 gcgcccggtg gcggccgcga cgagctcgac cgtgcggtcg tcgtgtgtcg ggccgagacc
     6661 gcccgagacg agcatcacat cggcgccgag tccttcttcg agcgcggcga gcagatccgc
     6721 gggcgcgtcc ccgacgatgg tgatccgcca cggctcgagg ccgatccgga gcgcctcgcc
     6781 agcgaagaac gggccgttgc ggtcgctccg ctcaccgcgc acgagctcgc tgccggtgac
     6841 gacgatggcg gcccgcggcc gcgtcaagag gaggcggccg tctgcacccc ggccggactg
     6901 acgacgaacg tcttgccgct cggcagctcg atgcggttcc cgttgagctt cagggccagg
     6961 ttcgcgggag agccaagtcg aacgtagagg ctcctacgtt cgaaccgctg cacctgcccc
     7021 tgctgaatcg tgccgcggta cagtggcttc ccggctcgtg agcccgcacg tacctccatg
     7081 aacgagtcgc cgcgcggggc gcgcacttcg agcttcgcga gcggcgccgc gttcgagctg
     7141 ccggagccgg cgccggcctg ggccagaccg ggaatgcgtt ccgtctccgg tccgccgaac
     7201 ttccaggcca cgaagacgag ggcggtcgcg accccgatga cgacgagcgc gagaacgagc
     7261 acgcgcgact cgcgtcgctc ccgctgccgc ccgacacgcg atgtcggcgc gcgatcgagc
     7321 ggggcggcgt cgtcctctcc gccgacgaag cgtgtgctgt attcgtcggc gtacagctgg
     7381 ccgtcgaggt cgagcgcgtc cgcgtacgtc cgcaagaagc ccctgacgta cgtgtggccc
     7441 ggcagctgct cgaactgctc ttcctcgagc gcccgcagat acttgcggcg gatcttcgtc
     7501 cgctgctcga gctcctccag gtcgagaccc tggcgaacac gcgcctcgcg caggctgctg
     7561 ccgatctcga acatgaaccg gtcagtctag tcgcatacgg cgagcgggcg gtcaccctcg
     7621 actgccggcc cactccagca ggccgtacac gcccacggag atggccgccg tcccagccag
     7681 gcacatgccg accactccga ggatgcggcc gaggcgggcc gctcccaggc cgcgcgtggg
     7741 cccgaccgac cgctcgtcga gccgcctgac cgagcgggcc gcgagcacgg ccacgagtcc
     7801 gagagcaaag ccaagaggga tggcgaagcc ggcgtggaga agctcgtacg tctcggagta
     7861 gcgggtggcg aggacggccg ccggcatcgt cgccgtcgag gcggcgccga gaagcaccga
     7921 aaggacggcc cgcccaaccc ctggggccgc ctcgctagct cttgacgtaa gggcgaccaa
     7981 tggctgcagg tgggatcgag cggccgatca ctccggcgac ggcgatcagc gtcaagacgt
     8041 agggcagcgc ttcgaacagc gtcccgaccg cggttgagta ctcctgcaga gcacgagcga
     8101 gggcgctcga gaacccgaac agcagacacg cggccgcgag gccgaagggc cgccaatttc
     8161 cgaagatcag cgcggcgaga gcaatgaagc cacgccccgc aatcatgttc tcggtgaagc
     8221 cgtcgacgaa gccgagtgag atgtaggcgc caccgcaagc cgcgagcacg cccgacatcg
     8281 tcacggccgc gtaccgggtg gcgtagacgt tgatccccac cgtgtcggcg gccttcgggt
     8341 gctctccgac ggagcggatg cgcaggccga tcggcgtctt gaagacgacg acgtacacga
     8401 cgaagaccat gatgatcgcg atccagatca tgaggttgag ctggccgagc acatcgtcga
     8461 ggaagttgcc gatgcccggt atcgagtccc agaagccgag gctcacgtcg gggatcgtgg
     8521 agaggtccgt cggggtcccg gcggtgccgt agatgtccac gaagaggtac ccggtgacgc
     8581 cgagcgcgag gaagttgatc gcggtacctc ccacgatctg atccgagcgc agcgtgaccg
     8641 agaagaatgc gtggacgaag gcggcgatgc cgccggcgag catgccgatc agcaacccga
     8701 gcacccagct gccggtcacg tcggagcccc agacggcgaa gaaggcgccc atgagcatca
     8761 tgccctcgag cccgatgttc acgacgccgg agcgctcgga gaagaggcca ccgagcgagg
     8821 caaagacgag cggcgtcgcg aagcggagca tcgatgcgaa gagcgcgccc cagacgacga
     8881 ccgcctcgag cttgccgacg ctggagaccg tggcgagata cccgatcgcc atgccgatca
     8941 cgccggc
//
LOCUS       DBNGNK010000402        14465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_930821,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000402 DBNGNK010000000
VERSION     DBNGNK010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 14465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14465
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_930821"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..830
                     /locus_tag="AC150V_11895"
     CDS             66..830
                     /locus_tag="AC150V_11895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR3763663.1"
                     /translation="MAQLDLTRFDAWLFDLDGVLTDTARIHSASWKTMFDEYLRARAE
                     ATGEPFAEFTQDDYLAYVDGKPRPAGVRDFLASRGISLPEGSLDDPPGEETVNGLGNR
                     KNLLVTGAIEAGEVEIYPASVDVLRMLRDTGAKTALVSSSRNAVAVIEAAGIDALFDI
                     RVDGVVAAELGLRGKPAPDTFLAAAERLDTMPERSVVVEDAVSGVQAGRAGGFGLVVG
                     IARHGNEDELRDAGAHRVVTDLSELTFVSTHGRSEQ"
     gene            827..3247
                     /locus_tag="AC150V_11900"
     CDS             827..3247
                     /locus_tag="AC150V_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HIR3763664.1"
                     /translation="MKPRRPVTPPPSRIYPVDHWAIVERELDLDRVPVTEALFALSNG
                     YIGMRATPEEGRPTFENGTYLNGVYENWEILYGESAHGFARTGQAIVNVADAKTIKLY
                     VDDEFFNPQSSEIVRWERRLNMRAGTLDREVVWQTPAGKHVQIVSRRLVSFAERHLAA
                     MWYEVTLLDGEEPIVISSELVVPSHDDLERDETELEDPRRGTVLPGRVLKPLLHVDRG
                     LRAVLVHRTAKSGIVTSCGMDHTIETTCEYRTYSKSTERFGQVVFEVDGRPGETIKLQ
                     KYAAYHHSFSASPEQLASRADWTLDRAKRHGFAAIAADHEAFAAGFWERSDVEVGLDQ
                     EMWENGGWLQQAIRFNLFHLLQATARADGAGVPAKGLTGAAYDGHYFWDSEIYVLPFL
                     VYTSPEAARNLLLHRYNQLGAARERARLLNQRGAKYPWRTINGEEASAYYAAGTAQYH
                     INADIAYALRRYVEVSGDTEFLADKGAEILVETARLWADLGFHSNRLEGQFVIHGVTG
                     PDEYTTVVNNNTFTNLMARENLSYAADVVEWLQREEPDHYEELVARTGLAPNELAVWR
                     RAADGMFIPFDERLGIHPQDDDFLDKEMWDFESTPLDAYPLLLHYHPLVIYRYQVIKQ
                     PDMVLAMFLLPDAFTPEQKRRNFTYYDSLTTGDSSLSAPIQAIMAIELGRAELAREYV
                     AHGVLMDLADVHGNARDGCHIAAMGGSWMIVTYGIAGLRDHDGRLEFDPRMPAGWRQL
                     RFRLQHQGRTLLVELSPETASYTLLDGDDLTLRHAGDELVLTRDEPTAERACHPELAP
                     AAGARSGK"
     gene            3271..3954
                     /locus_tag="AC150V_11905"
     CDS             3271..3954
                     /locus_tag="AC150V_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007572520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR3763665.1"
                     /translation="MGSFGLYLLFLIPPMIIGFIIQGRLNRTVKEQMQVAVSSGLTGR
                     QVAQRILEHNNLEVPVDTSPGGPLSDHYDPRHKSVHLSEPVYRESTVASVAIAAHEVG
                     HALQHAKGYSMFKVRSAMWPAVSFASKAWIFILLAGAFAGVLGLVQFAVVLFAVVVVF
                     QLVTLPVEFDASRRAMVQLADLGIVTESERAGAKRVLSGAAMTYVAAALAALSQLAYY
                     ALIFLGNRS"
     gene            3985..5154
                     /locus_tag="AC150V_11910"
     CDS             3985..5154
                     /locus_tag="AC150V_11910"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018832186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR3763666.1"
                     /translation="MRPIVFLIRAGAVVALLAVVFSLLDRDEPVQATSASAVESSAEA
                     AESQAEAQAAKKSAAAAAAKKKRASVKTPKSIVTIAAVGDIVMGNTPNLPPDGGRSFF
                     SDVQSELAADIVLGNLEGTLTTASGSKCGAGSQNCYAFKSPPAYARWLKRAGFTVLNL
                     ANNHAFDFGKAGLTETIQALEQQGLSHTGRPGQITYQQVGDASVAVVGFAPYPWAQSL
                     TDIAAAKQLVQKAAAKSDVVIVTFHGGAEGSSKQHVRPGTEFFLGENRGNLVKFSRAV
                     VDSGADVVIGHGPHVLRGMEWYKGRLIAYSLGNFAGYKVFSLGGPLSVSGILRLTLRG
                     DGAFETGILVPTRLVGAGLPALDPAEAAHGVVRNLSKEDFGSRRVKVAATGVLGR"
     gene            5162..5560
                     /locus_tag="AC150V_11915"
     CDS             5162..5560
                     /locus_tag="AC150V_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763667.1"
                     /translation="MAQLSAAARVSVCMTRSLRLTDLALVLGLLFLALVLVARHGAAP
                     ASTGPVGPLSVSEATSFFSNRPLAVRGFLVERDGRPLLCAGPACKGARLWVEGFRGRP
                     SDRAVLMIGVVDDRRITLVRLPRQERSARF"
     gene            5633..6988
                     /locus_tag="AC150V_11920"
     CDS             5633..6988
                     /locus_tag="AC150V_11920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR3763668.1"
                     /translation="MSEREFDVVVIGAGAPGEVAAGRLAERGLEVAIVEDRLVGGECS
                     FYACMPSKALLRPVELEAEVGRVPGLSAGPLDAAAVLARRDEVISGLDDSGQIPWLAE
                     RDITLVRGRGVLDGERRVRVGDELLVARRSVIVATGSAPLVPAIQGLVESAPWTNEEG
                     TTAKEIPARLAVLGGGPVAVELAQAWAALGSRVTLLNRGDRLLRREEPFAGEHVREGL
                     EAAGVDVRLSTVAARVSRNGSYELELTDGSSLDADVLLVAAGRVPRTDGIGLESVGLS
                     GGDWLSVDDSMRVPGYDWLYAVGDVNGRALLTHMGKYEARLAADVVLGASARVRWASA
                     DSPRVVFTDPQVGSVGLTLARAEEEGRPVKAVDVGTNANAGGSFVGRGAVGTSRLVVD
                     EDRGTVVGATFTGPEIAEALHAATIAVVAEVPLDDLWHAVPSFPTRSEVWLKLLEAYG
                     L"
     gene            complement(7000..7437)
                     /locus_tag="AC150V_11925"
     CDS             complement(7000..7437)
                     /locus_tag="AC150V_11925"
                     /inference="COORDINATES: protein motif:HMM:NF028004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HIR3763669.1"
                     /translation="MRLKAVIVVLVLGMFAAAAVVATAQTTNGLPKYTKNYKKWPKLN
                     KKLIRGGSSAHQGVKNVHASKKQNRKTKKFPNGTVIVKSIATPGKKGLPALVAVMRKV
                     KGNWQWREYSLSGKRYSRLAIPTSVCTGCHVGAKRKDYVFTAR"
     gene            7576..7866
                     /locus_tag="AC150V_11930"
     CDS             7576..7866
                     /locus_tag="AC150V_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763670.1"
                     /translation="MSEVVGREIERAEPKTARRPWRSLVIEALGPLTALAGVVWAIAQ
                     PYRIVFLHPDGKGFYDYLAQPPLLVVLVGLIFSLTIGRGLLKDLDGGNGSTR"
     gene            7850..8428
                     /locus_tag="AC150V_11935"
     CDS             7850..8428
                     /locus_tag="AC150V_11935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763671.1"
                     /translation="MAPRAEIVHWLFAAGFLLLGLVLLAEAVVGQGVWRKRRWRVYVW
                     PSIAFGLGVLLWPVAVFFTSSTLHMLAHTLWAEVALIAGAVQLAVAHGKLTSRWWNLT
                     MSAAMFASGVAFLIHEQNGWLFSRAAFLHHALGWVLVVAALVPLASTLQPRSNVWRAG
                     FGFTWIVIAVLLFADRDTAPIFGHLSDLAGGP"
     gene            8425..9756
                     /locus_tag="AC150V_11940"
     CDS             8425..9756
                     /locus_tag="AC150V_11940"
                     /inference="COORDINATES: protein motif:HMM:NF017262.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC/CopD family protein"
                     /protein_id="HIR3763672.1"
                     /translation="MRRLLALALALACVVLVSPSAASAHATLSWTTPATQASLDGVPN
                     EVQLHFDQSVTATETAITVYAVDGTVVSEPAFQPADKRDVIARVSGLERDSAYTVRWR
                     ATSADGHTVAGVYTFGIGVPAPPPTEAYGASGPSFSDDVARWAYFATLAVLLGGLGMR
                     LLVLRGPLPARLERRFYALIGIAALANVNVGVVAFLLRAEEALQLSIVDLLYGDLSPI
                     ATQTRFGHAFVAMTLGFAWVSALIALAWISDRLVLLWAAFVVTLALASGLSLSGHSAL
                     EPNSTVLTKSADWLHLVAASLWVGGVITLAVCVWPVAPELRKRAFLGFARLAMVLMAA
                     VVLAGTYLSIARMPAIADLWETSYGQVLLLKVGLVCLALAWGAVHHFVVRPRIERGRA
                     SGTIRRSLVGEGLVALAVLLVAAVLTNGEPPPTEPATGAEQATAAEGGPQR"
     gene            complement(9747..10196)
                     /locus_tag="AC150V_11945"
     CDS             complement(9747..10196)
                     /locus_tag="AC150V_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR3763673.1"
                     /translation="MASAPRVQAPGYYHVFTRATGGGAYFVDDGDRHVFRELLRRTAQ
                     QLDWQLHAYCLMTTHYHAVIEIRHPNLSRGMQRINSRYVRYFNERWSRFGSLVATRFK
                     SHVIEDEHYFAVVCRYVLMNPVTADLCRSPEGWPWSGGLVARDMSYL"
     gene            complement(10312..11295)
                     /locus_tag="AC150V_11950"
     CDS             complement(10312..11295)
                     /locus_tag="AC150V_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012237532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR3763674.1"
                     /translation="MRAMVLERAREPLRRVDLLEPAPAAGELLLRVVACGVCRTDLHI
                     VDGELDRPKLPLVPGHQIVGEVVADGSAASTSSGARRVGVPWLGWTCGRCSYCRAGAE
                     NLCDRARFTGYDIDGGFAEFAVADERFCFEIPAGYPDLQAAPLLCAGLIGYRSLRAAG
                     ERERLGLYGFGAAAHIVCQVAGWEGRRVFALTRPGDEAAQEFALSLGAAWAGPTEPGP
                     PEELDAAIIFAPAGELVPRALGAVRKGGVVVCAGIHMSDIPSFPYELLWGERTLRSVA
                     NLTRRDGEEFLSLAPQVPVRTEVEPFPLDAANVALDRLRAGDVRGAVALVP"
     gene            11404..13068
                     /locus_tag="AC150V_11955"
     CDS             11404..13068
                     /locus_tag="AC150V_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR3763675.1"
                     /translation="MPARADDTRVLHQIVSAVASTLELEEVLAAVVRLLSEGGGVHAC
                     FVYLLDERGERLVLRAAPGPYRHHEGDVALHRGEGLAWWAIEHGEPAFIRESALADPR
                     VKYVPELEEERYQSLLAVPVFSRDGDPIGVITAHTEAPREFSDAEADFLVTCASLVAG
                     AIENARHYEETRGRVEELEQLTELAEALAAAESVADVLAEGVRRARVLLGARACHLYV
                     LDASTEELERSASDPEVGRAPERIGLADLGPELARGGRSSRLSIPLVADGELLGLLVA
                     EKSARVELARAIGSQIAVALKKALLIERLAEKNLIKDFFEELAAGRARGELEGRAVQL
                     GCDLGQPHVVLIAEPPDSDLERSLRALGESCLLDRRDDVLRAVVRVPPAGADALLDRL
                     RRIHGGLERPVAVGVSSVCVGEESYANGFEEARQALIGTAVSGSGPSVTSYEELGPYK
                     YLLKVALDGGVRDATVDAVTRIAEYDERRGSSLVTTLEEFLRRRGNISATADALYVHP
                     NTLRQRLRRIGELSGLDLRRDDWLMVEIAVKMVRLEHALGTGRADP"
     gene            13157..14440
                     /gene="glnT"
                     /locus_tag="AC150V_11960"
     CDS             13157..14440
                     /gene="glnT"
                     /locus_tag="AC150V_11960"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III glutamate--ammonia ligase"
                     /protein_id="HIR3763676.1"
                     /translation="MADAQPTVDSVWKEIQQRGIEFAFAQFVDMYARPSAKLVPIRSK
                     SDLEGLVEDGAGFAGFAAGEIGQEPSDPDIAAIPDLASFTPVPWQPNLARFACDVTVD
                     GDSWAYCPRTILRRAVAAAEEKGYEFKFGCELEYFLVRQLDDGSIEIADRYDTLEKPC
                     YDMAGLTRRYDFLTTVSRYCNDLGWGNYANDHEDANGQFEQNFTFDHALTTCDRAIFF
                     RYMVHVLAEQNDMIATFMPKPFSHLTGNGCHFHMSLWDGETNLFLDEADPRGLGLSEL
                     AYNFIGGLKKHARSYSAFTAPTVNSYKRLKVGTTVSGATWSPVWISYGYNNRTQMLRI
                     PGPGRVEDRTVDGSCNPYLAAAAVLAAGIDGIEHGLDAGEPNTENLYRVPYDELRGRG
                     LEALPASLLAATDELQASDVMRAAFGPGRDEYYVE"
BASE COUNT         2213 a         4940 c         4939 g         2373 t
ORIGIN      
        1 tgctcgccgc gcgcgcttcc tgacgcacga cacccgccgc gaccgcaaga atgcgcgccg
       61 atcccgtggc ccagctcgac ctcacccgct tcgacgcctg gctcttcgac ctcgacggag
      121 tcctgacgga cacggcgcgg atccactctg cgtcctggaa gacgatgttc gacgaatacc
      181 tgcgcgcccg ggccgaggcg acgggcgagc cgttcgccga gttcacgcag gacgactacc
      241 tcgcctacgt cgacggcaag ccgcggcccg cgggcgtccg cgatttcctt gcctcgcggg
      301 ggatctcgtt gccggagggc tctctcgacg acccgccggg cgaagagacc gtcaacgggc
      361 tcggcaaccg gaagaacctc ctcgtgaccg gcgcaatcga ggccggcgag gtggagatct
      421 accccgcttc ggtcgatgta ctccgcatgc tccgcgacac cggcgcgaag acggctctcg
      481 tctcgtccag caggaacgcg gtcgccgtga tcgaagcggc gggaatcgac gccctcttcg
      541 acatccgggt cgacggggtc gtggcggcgg agctcggcct ccgtggcaag ccggctcccg
      601 acacgttcct cgccgccgcc gagcggctcg acacgatgcc cgagcgctcc gtggtcgtgg
      661 aggacgccgt gtccggggtt caggccggca gggcgggcgg cttcgggctg gtggtgggca
      721 ttgcccggca cgggaacgag gacgaactgc gcgatgccgg cgctcacaga gtcgtaacag
      781 acctttcgga gttgacgttc gtctctacgc atggaaggtc agaacagtga aacctcgtcg
      841 cccggtcacc ccgccaccgt ccaggatcta ccccgtcgat cactgggcga tcgtagagcg
      901 ggagctcgac ctcgaccgtg ttccggtcac ggaggcgctc ttcgccctct cgaacggcta
      961 catcgggatg cgcgcgaccc ccgaggaggg acggcccacg ttcgagaacg gcacctacct
     1021 caacggcgtg tacgagaact gggagatcct ctacggcgag tcggcgcacg gcttcgccag
     1081 aaccggtcag gcgatcgtga acgtcgccga cgcgaagacg atcaagctct acgtcgacga
     1141 cgagttcttc aatccacaga gctcggagat cgtccgctgg gagcgccgcc tcaacatgcg
     1201 tgcgggcaca ctcgaccgtg aggtcgtctg gcagacgccg gccgggaagc atgtccagat
     1261 cgtgtcccgt cggctcgtct cgttcgccga gcgtcatctg gcggcgatgt ggtacgaggt
     1321 gacgctgctc gacggcgagg agccgatcgt gatctcgagc gagctcgtcg tgccgtcaca
     1381 tgacgacctc gagcgcgacg agaccgagct cgaggacccg cgccgcggca cagtactccc
     1441 cggtcgcgtc ctgaagccgc tcctgcacgt cgatcgcggt ctacgagcgg tgctggtgca
     1501 ccggacggcg aagagcggca tcgtgaccag ctgcggcatg gaccacacga tcgagacaac
     1561 ctgcgagtac cgcacttact cgaagtcgac ggagcgcttc ggacaggttg tcttcgaggt
     1621 cgacggccgg ccgggtgaga cgatcaagct gcagaagtac gccgcctacc accactcctt
     1681 cagcgcgtcg ccggagcagc tggcctcgcg tgccgactgg acgctcgatc gcgccaagcg
     1741 ccacggtttt gccgcgatcg cggccgacca cgaggccttc gcggccggct tctgggagcg
     1801 cagcgacgtg gaggtcggac tcgaccagga gatgtgggag aacggtggct ggctgcagca
     1861 ggcgatccgg ttcaatctct tccacctcct gcaggcgacc gcccgcgcgg acggcgccgg
     1921 cgtgcccgcc aagggactta cgggtgcggc ctacgacggc cactacttct gggacagcga
     1981 gatctacgtg ctgcccttcc tcgtctacac ctcgccggag gcggcgcgga acctcctgct
     2041 ccaccgctac aaccagctcg gagccgcccg tgagcgcgcc cgcttgctca atcagcgcgg
     2101 cgcaaagtac ccgtggcgga cgatcaacgg cgaggaggca tccgcgtact acgcggccgg
     2161 tacggcccag taccacatca acgccgacat cgcctacgcg cttcgcaggt acgtcgaggt
     2221 cagcggcgac accgagttcc tggccgataa gggtgccgag atcctcgtcg agacggcgcg
     2281 actgtgggca gacctcggct tccactccaa ccgcctcgag ggtcagttcg tcattcacgg
     2341 cgtgaccggc ccggacgagt acacgaccgt ggtcaacaac aacacgttca cgaacctgat
     2401 ggcccgggag aacctcagct acgcggcgga cgtcgtggag tggctgcagc gtgaagagcc
     2461 ggatcattac gaggagctcg tcgcgcggac cggtctcgct ccgaacgagc tggccgtctg
     2521 gcgtcgggcg gccgacggga tgttcattcc cttcgacgag cgtctcggca tccatccgca
     2581 ggacgatgac ttcctcgaca aggagatgtg ggacttcgag tcgaccccgt tggacgccta
     2641 tccgctcctt ctccactatc acccgctggt gatctaccgc taccaggtga tcaagcagcc
     2701 ggacatggtg ctcgcgatgt tcctgctgcc cgacgcgttc acacccgagc agaagcggcg
     2761 caacttcacc tactacgact cgctgacgac cggcgactca tcgctgtcgg ctccgatcca
     2821 ggcgatcatg gcgatcgagc tgggccgtgc cgagctggcg cgggagtacg tagcccacgg
     2881 cgtgctgatg gatctcgcgg acgtgcacgg gaacgcccgc gacggctgtc acatcgcggc
     2941 gatgggcggc tcctggatga tcgtcaccta cggcatcgcc gggctgcggg accacgacgg
     3001 ccggctcgag ttcgatccgc gcatgcccgc aggctggcgt cagcttcgtt tccggttgca
     3061 gcaccaaggc cggacgctgc tggtggagct gtcccccgag acggcgtcct acacgctgct
     3121 cgacggcgac gacctgactc tgcggcacgc cggcgacgag ctcgtcctca cccgcgacga
     3181 gccgaccgca gaacgggcct gccaccccga gctcgcgcct gccgcagggg cccgcagcgg
     3241 caaatagtgc tggtgctact ctgatccgac atgggtagct tcggtctgta cctgctgttc
     3301 ctgatcccgc cgatgatcat cggcttcatc atccagggac gcctaaatcg caccgttaag
     3361 gagcagatgc aggtggccgt ctcgagcggt ctgacgggcc gccaggtcgc tcagcgaatc
     3421 ctcgagcaca acaacctcga ggtgcccgtc gacacgtcgc cgggagggcc gctgtccgac
     3481 cactacgacc cacggcacaa gtccgtgcac ctctccgagc ccgtctaccg ggagagcacc
     3541 gtcgcctccg tcgcgatcgc cgcgcacgag gtcggccacg cgctgcagca cgcgaagggc
     3601 tactcgatgt tcaaggtgcg ctcggcgatg tggcctgccg tctcgttcgc gtcgaaggca
     3661 tggatcttca tcctgctcgc gggagcgttc gccggcgtac tcggcctcgt ccagttcgcg
     3721 gtcgtcctct tcgccgtggt cgtcgtcttc cagctcgtga cgctgcccgt cgagttcgac
     3781 gcgtcacgcc gcgcgatggt gcagctggcc gacctcggca tcgtcaccga gagcgaacgg
     3841 gccggagcga agcgggttct ctcgggcgcg gcgatgacct acgtcgcggc ggcgctggct
     3901 gctctctcgc agctcgccta ctacgccctc atcttcctgg gcaaccgcag ctaggggatc
     3961 cgtcggccgc gccggcgcga gggcatgagg ccgatcgtct ttctcatccg cgcgggagca
     4021 gtcgtggcgc tcctcgccgt cgtcttctcc ttgctcgatc gcgacgagcc ggtgcaggcc
     4081 acgtcggcga gcgccgtcga gtcgtcggcc gaggcggcgg agtcgcaggc agaggcgcag
     4141 gcggcgaaga agtccgcagc cgccgccgcg gcaaagaaga agcgggcgag cgtgaagacc
     4201 cccaaatcga tagtcaccat cgccgccgtg ggcgacatcg tgatgggcaa cacaccgaac
     4261 ctgcccccgg acggagggcg gagcttcttc tccgacgtcc agagcgagct cgccgccgac
     4321 atcgtgctgg gcaatctcga agggaccctg acgacggcgt cgggctcgaa gtgcggcgcc
     4381 ggcagccaga actgctacgc gttcaagtcg ccgccggcgt acgcgcgttg gctgaagcgg
     4441 gccggcttca ccgtgctcaa cctggcgaac aaccacgcgt tcgacttcgg caaggccggc
     4501 ttgaccgaga cgatccaggc gctggagcag cagggcctga gccacaccgg ccggcccggc
     4561 cagatcacct accagcaggt cggggacgcc agcgtcgccg tcgtcgggtt cgcgccctac
     4621 ccctgggcgc agagcctgac cgacatcgcg gcggccaagc agctcgtgca gaaggccgcc
     4681 gccaagtccg acgtcgtgat cgtgacgttc cacggaggcg ctgaggggtc gtcgaagcag
     4741 cacgtcaggc cgggaaccga gttcttcctg ggcgagaaca gagggaacct cgtcaagttc
     4801 tcgcgcgccg tcgtcgactc gggtgcggat gtcgtgatcg gccacgggcc gcacgtcctt
     4861 cgcgggatgg agtggtacaa ggggcgcctg atcgcctact cgctcggcaa cttcgccggt
     4921 tacaaggtgt tctcgctcgg cggaccgctg agcgtgagcg gcatcttgcg cctcacgctg
     4981 cggggagacg gagcgttcga gaccggcatc ctcgtcccca cccggctggt cggtgcgggg
     5041 cttcccgcgc tcgacccggc cgaggcggcc cacggcgtcg tccgtaacct ctccaaggaa
     5101 gacttcgggt cgcgccgcgt caaggtcgcc gctacggggg tgctcggacg ctgaccgcct
     5161 gttggcgcaa ctctcggcgg ctgcgcgcgt ctcagtctgc atgacgcggt cgctccggct
     5221 gaccgatctc gcgctcgtcc tcggcctgct tttcctcgcc ctcgtgctcg tcgcacggca
     5281 cggcgcggct cccgcctcga cggggcccgt aggtccgctc agcgtgtccg aggcgacgtc
     5341 gttcttcagc aatcggcccc tcgccgtccg cggcttcctc gtcgagcgag acgggcggcc
     5401 gctcctgtgc gcagggcccg cgtgcaaggg tgcccggctc tgggtcgagg gcttccgcgg
     5461 ccgtcccagc gatcgcgcgg tgctcatgat cggcgtcgtc gacgaccggc gaatcactct
     5521 cgtccgcctc ccccgtcagg agcgaagcgc ccgtttctag cgtccgaggc gaccgtgcag
     5581 cccggcctca ctctgcaaga ctccgacgca tgtccgagct gggagggagc gggtgagcga
     5641 gcgggagttc gacgtcgtcg tgatcggggc cggtgccccg ggggaggtcg ctgcgggccg
     5701 gctcgcagag cgtggcctcg aggtcgcgat cgtggaggac aggctggttg gaggtgagtg
     5761 ctcgttctac gcgtgcatgc cttcgaaggc gctgttgcgt ccggtcgagt tggaggctga
     5821 ggtcggccgc gtgccgggat tgtccgcagg tccgctcgat gcagccgccg tcctcgcccg
     5881 tcgcgacgaa gtcatctcgg gcctcgacga ctccgggcag ataccttggc tcgccgaacg
     5941 ggacatcacg ctcgttcgtg ggcgcggcgt cctggacggc gaacggcggg tgcgagtggg
     6001 cgacgagctg ctcgtagcgc gccggtccgt catcgtcgcc accggcagcg cgccgctcgt
     6061 tccggctata caggggctgg tcgagtcggc accgtggacg aatgaggagg gaacgactgc
     6121 gaaggagatt ccggctcgcc tggccgtgct ggggggtggt ccggtcgcgg tggagctcgc
     6181 ccaagcgtgg gcggctctcg gctcgcgggt aaccctgctc aaccggggcg atcggctgct
     6241 gcgccgagag gagccgttcg ccggcgagca cgtccgcgag gggttggaag ccgctggggt
     6301 cgacgtccgc ctctcgaccg tggccgcccg cgtctcgcgg aacggctcgt acgagctcga
     6361 gctgaccgat ggatcgtcgc tcgacgccga cgtgctcctc gtcgccgcgg ggcgggtgcc
     6421 gcggacggac ggcatcgggc tcgagtcggt cggactctcg ggcggcgact ggctctccgt
     6481 cgacgacagc atgcgggtac cgggatacga ctggctgtat gcggtcggtg acgtcaacgg
     6541 acgcgcgctg ctcacacaca tgggcaagta cgaggccagg ctggcggccg acgtcgtact
     6601 cggcgcgagc gctcgcgtcc gctgggcctc cgccgactcg ccgcgcgtcg tcttcaccga
     6661 tccacaggtc ggatccgtcg gcctgaccct ggcgcgggcc gaggaggaag gtcgtccggt
     6721 caaggccgtc gacgtcggca ccaacgccaa cgccgggggc tcgttcgtcg gccggggggc
     6781 cgtcgggacg tcgaggctgg tcgtcgacga ggatcgcggc actgttgtcg gcgcgacgtt
     6841 cacggggccg gagatcgccg aggccctgca cgcggcgacc attgcggttg tggcggaggt
     6901 gcccctcgac gacctgtggc acgcggtgcc gtcgttcccg acgcgcagcg aggtctggct
     6961 gaagctgctc gaggcctacg gcctgtaggg ggcccctgcc tacctggcgg tgaagacgta
     7021 gtccttccgc ttcgcgccga cgtggcagcc cgtgcagacg gaggtcggga tcgccaaccg
     7081 cgagtagcgc ttgcccgaca gggagtactc gcgccactgc cagttgccct tcacctttcg
     7141 catgacggcg accaacgccg gaaggccctt ctttccgggc gtcgcgatcg acttcacgat
     7201 cacggtgccg ttcgggaact tcttcgtctt ccggttctgc ttcttgctcg cgtggacgtt
     7261 cttgacgcct tgatgcgcgc tcgatccacc cctgatcagc ttcttgttca gcttcggcca
     7321 cttcttgtag ttcttggtgt acttggggag cccgttggtc gtctgggccg tggccacgac
     7381 ggcggcggcc gcgaacatgc cgagcacgag gacgacgatg acggccttga ggcgcatctc
     7441 ggtgaatgat ggcgggcaag gacggacgga gccagggtcg aattcttaaa tcgccttacg
     7501 tttgcgaccg tgcgttctcc ggtgcacact tcgacatgtc ccgagagagt cgacaactgg
     7561 gtggggtgaa caagcatgag cgaagtcgtc ggcagggaga tcgagcgggc cgagccgaag
     7621 acggcgcgcc gcccgtggcg ttccctcgtg atcgaggcgc tggggccgtt gaccgcactc
     7681 gccggagtgg tgtgggcgat cgcgcagccc tatcgcatcg tgttcctgca tcccgacggc
     7741 aagggcttct acgactacct cgcgcagccg ccgctgctgg tcgtgttggt cggtctgatc
     7801 ttctcgttga cgatcggccg aggactgctc aaggatctcg acggaggcaa tggctccacg
     7861 cgctgagatc gtccactggc tcttcgcggc gggctttctc ctcctcgggc tcgtcctgct
     7921 ggcagaggca gtcgtcggac agggggtctg gcgcaagcgc cgatggcggg tgtacgtctg
     7981 gccgtccatc gcgttcggcc taggcgtact gctctggccg gtcgcggtct tcttcacgag
     8041 ctcgacgctg cacatgctcg cccacaccct gtgggcggag gttgcgttga tcgccggtgc
     8101 cgtccagctt gccgtcgcgc acggaaagct gacgagccgc tggtggaacc tgacgatgtc
     8161 ggccgcgatg ttcgcgtccg gcgtcgcgtt cttgattcac gagcagaacg gctggctctt
     8221 ctcacgtgcc gcgttcctcc accacgccct cggctgggtg ctcgtggtcg cggcgctcgt
     8281 acctctggcc tcgacgctgc agccgcgcag caacgtctgg cgagccggct tcggcttcac
     8341 ctggatcgtc atcgcggtgc tgctcttcgc cgaccgcgac acggcaccga tcttcggcca
     8401 tctctccgac ctcgccggag gaccgtgagg cgtctactcg ccctcgccct cgctttggcg
     8461 tgcgtcgtgc tggtctcgcc gagcgcggcg tcggcgcatg cgacgctcag ctggacgaca
     8521 cccgcgacgc aggcgagcct cgacggcgtg ccgaatgagg tacagctgca cttcgaccag
     8581 tccgtcacgg ctacggagac ggcgatcacg gtgtacgcgg tcgacgggac tgtggtatcg
     8641 gagcccgcct tccagccggc ggacaagcgg gacgtgatcg cccgcgtcag cggcctggag
     8701 cgggactccg cgtacaccgt ccgttggcgg gcaacctccg ccgacgggca taccgtcgcg
     8761 ggggtctaca cgttcggcat cggcgtgccg gccccgccgc ccaccgaagc ctacggcgca
     8821 tccggcccga gcttctccga cgacgtcgcc cgttgggcgt acttcgccac gctcgcagtc
     8881 ttgctgggcg ggttggggat gaggctgctc gtgctgcgcg ggcccctgcc ggctcggctg
     8941 gagcggcgct tctacgcgtt gatcgggatc gccgcgctgg ccaacgtcaa cgtcggtgta
     9001 gtcgcctttc tcctgcgtgc ggaggaggcg ctgcagctgt cgatcgtcga cctcctctac
     9061 ggcgacctct cgccgatcgc gacacagacc cggttcggcc acgcgttcgt cgcgatgacg
     9121 ctcgggtttg cctgggtctc cgccctcatc gctctcgcgt ggatctccga ccggctcgtg
     9181 ctcctctggg cggcgttcgt cgtgacgctt gcgcttgcgt ccgggctgtc gctctcgggg
     9241 cactcggctc tcgagccgaa ctccaccgtg ttgaccaagt ccgccgactg gctgcacctc
     9301 gtcgccgcct cactgtgggt cggtggcgtg atcacgctcg ccgtgtgtgt atggcccgtc
     9361 gcgccggagc tccggaagcg ggcgttcctc gggttcgccc gtctggcgat ggtgctgatg
     9421 gcggcggtag tccttgccgg aacgtatctc agcattgcgc gcatgccggc gatagctgat
     9481 ctctgggaga cgagctacgg ccaggtgctg cttctcaaag tcggactggt gtgcctcgcc
     9541 ctggcgtggg gagcggtgca ccacttcgtc gtccggcccc ggatcgagcg ggggcgtgcc
     9601 tctggaacga tccgtcgcag cctcgtcggc gagggcctgg tcgctctcgc cgtgctgctc
     9661 gtcgccgccg tcctcacaaa cggcgagccg ccccccaccg agccggccac cggggctgag
     9721 caggcgaccg cggcagaagg gggacctcag aggtaggaca tgtccctggc gaccaggccg
     9781 ccgctccaag gccagccttc cgggcttctg cacaagtcgg ctgtcacagg gttcatgagc
     9841 acgtagcggc agacgacagc gaagtagtgt tcatcttcga tcacgtgtga tttgaagcgc
     9901 gtcgcgacga gcgagccaaa tcgtgaccag cgctcgttga agtagcggac gtacctcgag
     9961 ttgatgcgct gcatcccgcg cgagagattc gggtgtcgta tctcgatcac cgcgtgatag
    10021 tgcgtcgtca tgaggcagta ggcgtgcagc tgccagtcga gctgttgtgc cgtgcgccgc
    10081 agcagctcgc gaaacacatg gcggtcgccg tcgtcgacga agtaggcgcc gcccccggtc
    10141 gctcgcgtga agacgtggta gtagccgggt gcctgaactc ggggcgcgct cgccatcgat
    10201 cgaccgtaag gggtcgatct cgcgcaccga gcgctccggt tgagaattca cacttgcgac
    10261 acgcttgagc gaggaggccg ttactcaggt cctcagaggt tgggcacggc tctacgggac
    10321 gagggcgaca gcgccgcgga cgtcgccggc gcgcagccgg tcgagtgcca cgttcgcggc
    10381 gtcgagcggg aagggctcaa cctccgtccg cacgggaacc tgcggcgcga ggctgaggaa
    10441 ctcctcaccg tcgcggcgcg tgagattggc gacggagcgg agcgtgcgct ctccccacaa
    10501 cagctcgtac ggaaaggacg ggatgtcgct catatggatt cccgcgcaca cgaccacacc
    10561 cccctttcgc accgcgccca gcgctcgagg gacgagctcg cccgcgggag cgaagatgat
    10621 cgcagcgtcg agctcctccg gggggccggg ctcggtcggc ccagcccagg ctgctccgag
    10681 cgacaacgcg aactcctgcg ccgcctcgtc gcccggccgc gtcaacgcga atacccgccg
    10741 accctcccac ccggcaacct ggcagacgat gtgcgcggcg gcgccgaagc cgtacaggcc
    10801 aagccgctcg cgttcccccg ccgcgcggag cgagcggtac ccgatgaggc cggcacagag
    10861 aagcggtgcc gcctgcagat cggggtaccc ggcgggaatc tcgaagcaga agcgctcgtc
    10921 cgcgacggcg aactcggcga agccgccgtc gatgtcgtac ccggtgaagc gggctcgatc
    10981 gcagaggttc tcagcaccgg cccggcagta cgagcaccgg ccgcacgtcc agccgagcca
    11041 ggggacaccc acgcgacgtg cgccgctcga cgtcgaggcc gcactgccgt ccgccacgac
    11101 ctcaccgacg atctgatggc cgggcacgag gggcagcttc ggccgatcga gctcgccgtc
    11161 gacgatgtga agatcggtcc gacacacacc gcaggccacg actcggagga gcagctcgcc
    11221 cgcagccggc gccggctcaa gcaggtcgac acgtcggagc ggctcgcggg ctcgctccag
    11281 caccatcgcc ctcatgcggc gctgttgtgg cggccggtca ctcccaccga tcctgtccga
    11341 aggagtcgcg cttcgcaacc caggcgcttt acagccctcg tagagcgatc gtacgatcag
    11401 ccaatgccgg cacgtgccga cgacactcgg gtgctccacc agatcgtctc ggcggtcgcc
    11461 tcgacactcg agctggagga ggtgctggcg gcggttgtgc gcctcctttc cgaggggggc
    11521 ggtgtccatg cctgtttcgt ctacctgctc gacgagcgcg gcgagcgcct cgtgctccga
    11581 gccgctccag gcccctaccg gcaccacgag ggcgacgtcg ccctccaccg cggcgagggg
    11641 ctcgcgtggt gggcgatcga gcacggcgag cccgcattca tccgcgagag cgcgctcgcc
    11701 gatccgcgtg tgaaatacgt cccggagctc gaggaggagc gctaccagtc gctgctcgcc
    11761 gtgccggtgt tcagccgaga cggcgacccg atcggagtca tcacggccca caccgaggcg
    11821 ccgcgggagt tcagcgacgc cgaggccgac ttcctcgtca cgtgcgcatc cctcgtcgcg
    11881 ggcgcgatcg agaacgcccg tcactacgag gagacgcggg ggcgcgtgga agagctcgag
    11941 cagctgacgg agctggcgga agctctcgcc gcagcggaga gtgttgccga cgtcctcgcg
    12001 gagggagtac gccgtgcgcg cgtgctcctc ggcgcgcggg cctgccatct ctacgtgctg
    12061 gacgcgtcga ccgaggagct cgagcggagc gcctcggatc ctgaggtcgg ccgggcgccg
    12121 gagcggatcg gcctcgcgga tctcggcccc gagctggccc gcggcggtcg ttcgtcgcgg
    12181 ctctcgatcc cgctcgtcgc ggacggtgag ctcctcggac tgctcgtcgc cgagaagagc
    12241 gcccgtgtcg agcttgcgcg ggcgatcggc agccagatcg cggtcgccct gaagaaggca
    12301 ctgctgatcg agcgcctggc cgagaagaac ctgatcaagg acttcttcga ggagttggcg
    12361 gccggacggg cccgaggtga gctcgagggc cgggccgtcc agcttggttg cgacctgggg
    12421 cagccgcacg tcgtcctcat cgcagagcca cccgattccg atctcgagcg gtcgctgcgc
    12481 gcgctcggcg agtcctgcct gctcgaccga cgtgacgacg tccttcgcgc cgtcgtccgc
    12541 gtccctccgg ccggggccga cgccctgctc gaccgactca gacgcattca cgggggtctg
    12601 gagcggccgg tcgcggtcgg cgtctcgagc gtgtgcgtag gagaggagag ctatgcgaac
    12661 ggcttcgagg aggctcggca ggcgctgatc ggcacagccg tcagcgggag cgggccgtcc
    12721 gtgacgagct acgaggagct cggcccctac aagtacctcc tcaaggtggc gctcgacggc
    12781 ggcgttcgtg acgcgacggt cgacgccgtg actcggatcg ccgagtacga cgagcggcgc
    12841 ggctcgtcgc tcgtcaccac actcgaggag ttcctccgac ggcgcggcaa catctcggcc
    12901 accgccgacg cgctctacgt ccaccccaat acgctccgcc agcggctccg ccgcatcggc
    12961 gagctgtccg gactcgacct ccgccgcgac gattggctga tggtcgagat cgcggtcaag
    13021 atggttcgcc tggagcacgc actgggcacc ggccgcgccg atccatagct ccaccgcgag
    13081 gtgtgtgccg gcacaccctg gctccccggc aacggcgatg gtagaagatg caacccacct
    13141 cgagggagga gatgccatgg ccgatgccca gccgacagtc gattcggtct ggaaagagat
    13201 ccagcagcgg ggaatcgagt tcgccttcgc ccagttcgtg gacatgtacg cgcggccgag
    13261 cgccaaactg gtgccgatca ggtcgaagtc cgatctggag ggcctggtcg aggacggagc
    13321 cgggttcgcg ggcttcgcgg cgggggagat cggtcaagag ccgagcgatc ccgacatcgc
    13381 agcgatccca gatctggcca gcttcacacc ggtgccatgg cagccgaacc tcgcccgttt
    13441 cgcgtgtgac gtcacggtcg acggagattc ctgggcgtac tgtccccgca cgatcctgcg
    13501 gcgcgcggtc gccgcggcgg aggagaaggg gtacgagttc aagttcggct gcgagctcga
    13561 gtacttcctg gtgcggcagc tggacgacgg ctcgatcgag atcgcagacc gctacgacac
    13621 gctcgagaag ccctgctacg acatggctgg tctgactcgc cgctacgact tcctgacgac
    13681 ggtctcccgc tactgcaacg accttggctg gggcaactac gccaacgacc acgaggacgc
    13741 gaacgggcag ttcgagcaga acttcacttt cgaccacgcg ctcacgacct gcgaccgtgc
    13801 gatcttcttc cgctacatgg tgcacgtgct cgcggagcag aacgacatga tcgccacgtt
    13861 catgcccaag ccgttctccc acctgaccgg caacggctgc cacttccaca tgtcgctctg
    13921 ggacggcgaa acgaatctct tcctcgacga ggctgatccc cgcgggctcg ggctctccga
    13981 gctggcatac aacttcatcg gcggcctcaa gaagcacgcc cgctcctaca gcgctttcac
    14041 tgccccgacc gtcaactcgt acaagcgcct gaaggtcggg acgacggtgt ccggtgccac
    14101 ctggtcgccg gtctggatca gctacggcta caacaaccgc actcagatgc tgcggattcc
    14161 ggggcccggc agggtcgagg accgaacagt cgacggctcg tgcaacccct atctcgccgc
    14221 agctgcggtt ctcgccgcgg gcatcgacgg gatcgagcac ggcctcgacg caggcgagcc
    14281 gaacaccgag aacctctacc gggtgcccta cgacgagctg cggggtcgcg ggctcgaggc
    14341 gctgcccgcg agcctgctcg ccgcaaccga cgaactccag gcgagcgatg tgatgcgtgc
    14401 ggccttcggg cccggacgag acgagtacta cgtagaataa tacataatcg tcaataggtc
    14461 tgagt
//
LOCUS       DBNGNK010000403         3298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1933964,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000403 DBNGNK010000000
VERSION     DBNGNK010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1933964"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="AC150V_11965"
     CDS             complement(<1..465)
                     /locus_tag="AC150V_11965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763677.1"
                     /translation="MRRALAIGVLTLLAGALLVGCGGSGGESTPLPSTTPAPPQRAEL
                     GWVERHEEDDESVEFRVDALEVTEDGWRAEVEITNQTSADYALGDPDASLDRRFGLML
                     FKTGDLRELEQRNEADDLPGLRRAQSFDPPLPLVIDSGSSWSGTISARGALAA"
     gene            509..1654
                     /gene="sucC"
                     /locus_tag="AC150V_11970"
     CDS             509..1654
                     /gene="sucC"
                     /locus_tag="AC150V_11970"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR3763678.1"
                     /translation="MDLYEYQGKKLFARFGIPVSEGRLATSPTEAGAAAQELAGGPVV
                     VKAQVLTVGRGKAGGIKLAENSREAQERAKEILGLDIRGHVVHQLWVERASDIAKEYY
                     LSITFDRGAKKALFMFTTKGGVDIEEVAATDPDALVRLHVDPLEGFKPYQSRRLIYEA
                     GVEDVGEQKQIAAIVGKLYDAFVGCDAMLCEINPLIVTPEGEVRALDSKFTIDDNALF
                     RHPELAGMRDLAAADPLEALAREKGVTYVKLDGEVGVLGNGAGLTMSTVDVVTFAGGR
                     PANFCDLGGGGDAQGVVDALEVITSDEQVRAIFFNIFGGITRGDEVARGILQALSDIS
                     IDHPIVVRLDGTNAEEGRSILADAAPANLHVEPTMLDAARKAVELSG"
     gene            1651..2289
                     /locus_tag="AC150V_11975"
     CDS             1651..2289
                     /locus_tag="AC150V_11975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763679.1"
                     /translation="MSDWSARAQAYIDSDAHREGDDLDALVAWAAGAKTGLDVATGGG
                     HVARRLREAGLEVVTCDPAPGMKPDVICTAESLPFADGAFDVVACRTAAHHFQDVRAA
                     VREMARVAARHVLLVDTVNMGDQMEEAERLRDPSHVRNYTDAEWRDVVLEAGLGVDEV
                     RFFEHTFDLQAWLDRTGCEGETAERVVTLLGERAAGGRITLDKIAIRAVKGY"
     gene            2301..3170
                     /gene="sucD"
                     /locus_tag="AC150V_11980"
     CDS             2301..3170
                     /gene="sucD"
                     /locus_tag="AC150V_11980"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR3763680.1"
                     /translation="MAIIVDSATKLVVQGLTGSEGRFHGLRNRSYGTLVVAGVTPGKG
                     GQDVDGIPVFDTVAAAVAEHGANTAMVFVPAPFAADAVYESVDAGIATVICITEGVPA
                     HEMLRLHAYIREKGVTMIGPNCPGVLSPGQANVGIIPAEIFRAGAVGLVSRSGTLTYQ
                     IGHELTQLGLGNSTIVGIGGDPIVGSSFIDVLARFEGDAQTEYVVLVGEIGGDEEEKA
                     AAFIQEEMSKPVVAYIAGFTAPPGKTMGHAGAIISGSSGTAQAKKDALEAKGIRVGTT
                     PTETAEIVAEIAG"
BASE COUNT          536 a         1084 c         1173 g          505 t
ORIGIN      
        1 agcggcgagc gctccacgag ccgagatcgt ccccgaccac gacgatcccg aatcgatgac
       61 aaggggcagg ggcgggtcga agctctgagc gcgccgcagt cccgggaggt cgtccgcctc
      121 gttgcgctgc tccagttcgc ggagatctcc ggtcttgaag agcatgaggc cgaagcgccg
      181 atcgagggac gcgtcgggat cgccgagcgc gtagtcggcc gaggtttggt ttgtgatctc
      241 gacctcggcg cgccagccgt cctcggtcac ctcgagcgcg tcgacgcgga actcaacgct
      301 ctcgtcgtcc tcctcgtggc gctcgaccca gccgagctcg gctcgctgag gcggcgccgg
      361 cgtggtgctc gggagcgggg tgctctcgcc gccgctgccg ccgcagccga cgagcagcgc
      421 cccggccagc agggtgagaa ctccgatcgc gagcgcgcgt cgcatgcggg aatgctcgca
      481 caagcctgct ggtaggctcg cgtgcgcctt ggatctctac gaataccagg gcaagaagct
      541 cttcgcgcgc ttcgggattc ccgtgtcgga gggacggctt gccacgagcc cgaccgaggc
      601 tggcgcggct gcccaggagc tcgccggtgg gccggtggtc gtgaaggctc aggtgctcac
      661 ggtgggccgc ggcaaggccg gcggcatcaa gctggcggaa aattcgcgtg aggcgcagga
      721 acgtgcgaag gagatcctcg ggctcgacat ccgcggccac gtcgtgcacc agctctgggt
      781 ggagcgggcg tcggacatcg cgaaggagta ctacctgtcg atcacgttcg accgcggcgc
      841 caagaaggcc ctcttcatgt tcacgacgaa gggcggcgtc gacatcgagg aggtcgcggc
      901 gaccgacccc gacgcactgg tgcggctcca cgtcgacccg ctcgagggct tcaagccgta
      961 ccagtcccgc cggttgatct acgaagccgg cgtcgaggac gtcggcgagc agaagcagat
     1021 cgcagcgatc gtcgggaagc tctacgacgc gtttgtcggc tgtgacgcaa tgctctgcga
     1081 gatcaacccg ctgatcgtca caccggaggg cgaggtccgt gcgctggact cgaagttcac
     1141 gatcgatgac aacgcactct tccgtcatcc cgagcttgcg gggatgcgcg atcttgccgc
     1201 cgccgatccg ctcgaggccc tggcccgcga gaagggcgtc acgtacgtca agctcgacgg
     1261 tgaggtcggc gtgctcggaa acggggccgg gttgacgatg tcgacggtag acgtcgtcac
     1321 gttcgccggc gggaggcctg cgaacttctg cgatctgggg ggcggcggcg acgcgcaggg
     1381 agtggtcgac gcgctcgagg tcatcacgag cgacgaacag gtgcgggcga tcttcttcaa
     1441 catcttcggg ggcatcactc ggggagacga ggtcgcacgg ggcatcctgc aggcgctgag
     1501 cgatatctcg atcgaccatc cgatcgtcgt tcgcctggac ggcacgaacg ccgaagaggg
     1561 ccgatcgatt cttgccgacg cggcgcccgc caacctgcac gtcgagccca cgatgctcga
     1621 tgccgccagg aaggcggtgg agctgtcggg atgagcgatt ggtcggcccg cgcccaggcc
     1681 tatatcgact cggacgccca ccgcgagggc gatgacctcg acgccctcgt cgcgtgggcg
     1741 gccggtgcaa agaccggcct ggacgtcgcg accggcggag gtcacgtcgc ccgccggttg
     1801 cgcgaggcag gcttggaggt cgtcacctgc gatcccgcgc ccggcatgaa gcccgatgtg
     1861 atctgcacgg cagagagcct tccgttcgcc gacggcgcct tcgacgtcgt cgcctgccgc
     1921 accgccgcgc atcacttcca ggacgtccgg gccgccgtcc gcgagatggc gcgcgtcgcc
     1981 gcgcgtcacg tcctgctcgt cgacacggtg aacatgggcg accagatgga agaggccgaa
     2041 cggctccgcg acccctcgca cgtccggaac tacacggacg cggaatggag agatgtcgtc
     2101 ctcgaggcag gtctcggtgt cgacgaggtc cgtttcttcg agcacacctt cgacctccag
     2161 gcctggctcg accgcacggg ttgcgagggc gagacggccg agcgcgtcgt caccttgttg
     2221 ggcgagcgcg ccgcaggtgg gcgaatcacg ctcgacaaga tcgcgatcag ggcggtgaag
     2281 gggtactagc ccgcgaggac gtggccatca tcgtcgacag cgcaacgaag ctcgtcgtcc
     2341 agggtctcac cggcagcgaa ggtcgcttcc acggcctccg caaccggtcg tacggaacgc
     2401 tggtcgtggc gggagtcacg ccgggcaagg gcgggcagga cgtcgacggg attcccgtct
     2461 tcgacaccgt cgccgcggct gtcgcggaac acggggcgaa cacggcgatg gtgttcgtgc
     2521 ctgctccctt cgcggccgac gccgtctacg agtcagtcga tgccggaatc gccacggtga
     2581 tctgcatcac ggagggagtg cccgcccacg agatgctccg gctgcacgcc tacatccgag
     2641 agaagggcgt cacgatgatc ggcccgaact gcccgggcgt gctgtcgccg gggcaggcga
     2701 acgtcggcat cattcccgcc gagatctttc gcgcgggggc ggtcggtctc gtctcccgct
     2761 cgggcacgct cacctaccag atcggccacg agctgacgca actcggcctc ggcaactcga
     2821 ccatcgtcgg catcggcggc gatccgatcg tcggctcttc gttcatcgac gtgctggcgc
     2881 gcttcgaggg cgacgcacag acggagtacg tcgtcctcgt gggagagatc ggcggcgacg
     2941 aggaggagaa ggccgccgcc ttcattcagg aggagatgtc aaagccggtg gtcgcctata
     3001 tcgcggggtt caccgcgccg ccgggcaaga cgatgggcca tgcgggcgcg atcatctcgg
     3061 gctcgtccgg caccgcccag gcgaagaagg acgcgctcga ggcgaagggc attcgggtcg
     3121 ggaccacgcc gaccgagacc gccgagatcg tcgcggagat cgctggctga tgcccctccg
     3181 gcccgagccg gtacccgatc tcgactggag tccggagcgg gctcgcgcct tcgccgacca
     3241 gacggtcgac ctctgggagg agttgctgac gcgactccga gacgctccgg tcgccggt
//
LOCUS       DBNGNK010000404        27962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1502840,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000404 DBNGNK010000000
VERSION     DBNGNK010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 27962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 27962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27962
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1502840"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="AC150V_11985"
     CDS             complement(<1..1237)
                     /locus_tag="AC150V_11985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR3763681.1"
                     /translation="MAGRVSQTAQLPASPDEQSFPVGRNIALLAACLTSLSGMLQLAA
                     AVATITLVLVTGVESILGLGPAIFLAAGALAAFPAGRAMDRVGRMPVIATGFLVGAAS
                     CAVTALGCATASAALVVLGLVGVGITSGTILLSRAAAADMVPPPRKAHAISLVLFGAL
                     FGALLGPLVFRPLFAGKEIELDSLIVPWLAAGAISLVGLALALAVRPDPRTIAIALDT
                     GESGGVSTAPLTEILRRPGVLVALAATVASFAVMVSVMNLTGYIIVGNDHPQADVFTV
                     ISVHIVGMYALVLFVGALVERIGRRTAAVAGLVLMAASTLPLWWAGSILWMSVSLFGL
                     GLGWSLSYVASAAELVTRAAPSERGRLVGFTDLVAGLLGAALALLGGVALTQIGVAAV
                     AIGATALVAAPALVIAWRRP"
     gene            1236..2012
                     /locus_tag="AC150V_11990"
     CDS             1236..2012
                     /locus_tag="AC150V_11990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HIR3763682.1"
                     /translation="MACILLDIDGVLHVSGEAIPGSVDAVAELRKSGHSLRFVTNNST
                     LPRAALADELRSLGFELDEEEIQTTPRTAAGELAGKRVFALVMPPVVADLEGLELSAM
                     DVEAVLIGGCDETLEPNQVFTYMNLARAFAEIQAGAEFYCLHKNKWWQTRRGPMLDAG
                     AFVAGLEYATGVEATVVGKPSPAYFASALDALEAEPELTWMVTDDIEQDVRGAQLFGM
                     RTVLLRTGKFRPETLEASGVTPDVVLSSLAQFPDWLERHL"
     gene            2012..2389
                     /locus_tag="AC150V_11995"
     CDS             2012..2389
                     /locus_tag="AC150V_11995"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR3763683.1"
                     /translation="MTAPGALRVGMDLIEIERIRRALDRPGFRQRCFTEAEQAYCESK
                     RNSAESYAARFAGKEAVGKALGCGVYFTWKEIEIAGRPKPGVRLFGKTAAWAEKVEAG
                     RIDLSMTHSRELAAAIAVVQEKA"
     gene            2386..3744
                     /locus_tag="AC150V_12000"
     CDS             2386..3744
                     /locus_tag="AC150V_12000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR3763684.1"
                     /translation="MILEPLYTAAEMRAAEERYPDYPESIPELMERAGRALAEESMHA
                     FPGVRRIAVVCGGGSNGGDGRVAARVLREAGLEAEETDVLDGADVVIDALFGTGFRGA
                     PRPEAAALIDRINSAGVPVVACDLPSGVNATTGEVEGAAVRASLTVSFHAPKVGLVVA
                     PGRRHAGRVVVADIGLEDTSTAATRATPEVLSLVPRRSAVDSKYSAGTVLVVGGSPGM
                     TGAAALAATAALRADAGYVILAVPAVSLAVLEILTLEPVKIGFDWESGVEAVLAAAER
                     AGAVALGPGLGRSGEARGLVRALLERLERPVVVDADALYRLEPLERAAPTVLTPHAGE
                     LARLLETTSSEVDAARLDAARQAAERFGAVCLLKGADTIVASPDAGPIVCDHGPPSLA
                     TAGTGDVLTGVVAAFLAKGVEPRLAAAAAATAHGLAAASIQHQAGVVAGDVAAALPAV
                     LS"
     gene            3749..4612
                     /locus_tag="AC150V_12005"
     CDS             3749..4612
                     /locus_tag="AC150V_12005"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3763685.1"
                     /translation="MRVEILGSGGATTTPRPGCTCRVCVEARKQGPPHSRTGPSAFVH
                     GPNVLFDTPEESKLQLDRAGIGEISACFYSHWHPDHTMGRRVFETRNGDFRAWPPEAK
                     RQLTTDVYLPQQVAADFRERLGAMDHLEFMQERGWVRIRELSDGDTVTIGGVEIRPFR
                     VAEDYVYAFELRGGGRRLLLAMDELNNWTPPRAVRGCDLAVIPMGICEFDPLTGQRRI
                     APEHPILRFEATHDETLEIVDLLRADRVVLSHIEEIDGLTVDDLHDLETRLRDGGRAV
                     TFAYDGLIVDL"
     gene            4653..5672
                     /gene="alr"
                     /locus_tag="AC150V_12010"
     CDS             4653..5672
                     /gene="alr"
                     /locus_tag="AC150V_12010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR3763686.1"
                     /translation="MARSAFTIDLGAVRHNVRALLDILDGAELWAVVKAEGYGHGAID
                     VGRAALQAGAHALCVATVPEAVALRASLREPRILVMGPSTSAEVAQAREARLELAVVG
                     GPLPEGVPVHVKLDTGMGRWGLAELPALGPQVVGLMSHFASADSDAAFSRRQLERFAA
                     ATASHARLTRHIANSAGALTLPDARLDAVRCGIALYGVSPFGGDPADHGLRPVMRWES
                     ELAHTRLLAAGESTGYGRTFVARRPTWIGLVPVGYADGFRRDMTGTEVSVGGERCPVV
                     GTVSMDVVAVELPAERPAGTVVTLLGDGITAERHAAVADTIGYEILCGMNAKAPRATR
                     EVVGE"
     gene            5665..6345
                     /locus_tag="AC150V_12015"
     CDS             5665..6345
                     /locus_tag="AC150V_12015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR3763687.1"
                     /translation="MSDHVQRRLALQERRAEALAARTERMLAPFTGRERALDAGCGAG
                     ALSFAIAPHVAEVVGIDRDEGLVAAARERAPANCTFVDGDLLSLPFEWGEFDLSGCAR
                     VLHHVRRPELVMAEIARVTRPGGRILIVDQLASVDPVAAIEHDRFERARDRSHNRLLP
                     DTDIRSLLDMNDLVVLENEVEHEQRDVDSYLELAGIPEDQRERLRRMAPADVYDVEIG
                     WYVARKRG"
     gene            complement(6581..7375)
                     /locus_tag="AC150V_12020"
     CDS             complement(6581..7375)
                     /locus_tag="AC150V_12020"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3763688.1"
                     /translation="MSATPTRRVAVVTGASSGIGAATARLLSTRGWHCLLMARRQEPL
                     SALAAEIGGEVEVCDISDRAAVDAAAERIRLRHPALHLLVNNAGFLVRGSFAEVDLDL
                     VESAMQVNYLGGVWATRAFLPGLRASAADGDAHIVNVASIGGTVAFAPAGAYSASKHA
                     QVAFSRSLRAVLAGSGIDVHTVMPGFVTTPGFPHPSLFRTRLGRRFVVGPEETARAIV
                     SAVERGRAEVVVPWFPYRLGAVAQALFPTLTTRVLAVARYPGDEPR"
     gene            complement(7387..8145)
                     /locus_tag="AC150V_12025"
     CDS             complement(7387..8145)
                     /locus_tag="AC150V_12025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR3763689.1"
                     /translation="MTGASSGIGEATARELARRGWRCVLVARREERLRALAAEIEGEA
                     APCDLADRDALAALAASLVHRHPSLHLLVNNAGIPARGTVLDLPSEAIERVLAVNFSS
                     GVRLTRLLLPSLRTAAATGSAHVVNVASVAGTMAFAQAGAYAASKHAQVAFSRALRST
                     LWPEGIEVHTVLPGFVETEGFPQRAMLAHQLGRRIVVRPPHVARAIVRAVEAGRAEVV
                     VPWFPYRLAGLVQAILPAVAARMARRTIGPSRPL"
     gene            complement(8151..9026)
                     /locus_tag="AC150V_12030"
     CDS             complement(8151..9026)
                     /locus_tag="AC150V_12030"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR3763690.1"
                     /translation="MRAYAHDLFTYPLPEGHRFPLGKYRLVRERAERLSGVEGHAARA
                     AVDDELERVHDDHYLGRVARGGLSRREELALGLPWSPELVERAKRSTGATVQAAEAAL
                     ADGVAMNLGGGTHHAFADAGRGYCVFNDVVTARRALTGRVRRVLVLDLDVHQGDGTHA
                     LLAADADAFTCSVNGFRNYPFKRVPGDLEVELVDGTGDDAYLEALGALVPQALARSRP
                     ELCFYLAGADPYAGDRLGRLALSIEGLRRRDELVRDALRAAGVPICVTLAGGYAEDIR
                     DTVEINLGTVRTFSA"
     gene            9057..9836
                     /locus_tag="AC150V_12035"
     CDS             9057..9836
                     /locus_tag="AC150V_12035"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR3763691.1"
                     /translation="MAGRLGGKQALVLGVANKRSIAWAIARRLHEEGAELAFTYQGER
                     IEKTVRDLAATVDGKLVTECDVRSDDDIERVFAETADVFGGGLDVLVHSVAFAAAEDL
                     EGRFADTPRDRFWMALDISAYSLVACARAAEPLMEARGGGSILTMTYLGGERAVPHYN
                     VMGVAKATLDSSVRYLAWDLGKKGIRVNAVSAGPVRTLAARSIAGFTTMETMFEERAP
                     LHRHIDADDVGAAAAYLLSEDARNVSGTTLYVDSGYHAMGM"
     gene            complement(9869..10840)
                     /locus_tag="AC150V_12040"
     CDS             complement(9869..10840)
                     /locus_tag="AC150V_12040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR3763692.1"
                     /translation="MNLPDGYRGRVPTSDDLVAVAEVLQADDVDDSGKIVLDEHFVHD
                     EWSRVGFDLAVDAWVVTDTNGTIVGYGQAALAERALVVSWGVVHPEHRGRGIGSAVLH
                     WIEMRASQQLAGLRSARFRHAVNASDPTAAALLAARGLRPIRHFWHLQIDLQEASEPE
                     PAAEGILIAGISSPGDLRAVHAVLDDAFADHWGHEPEPFDRWTETQTASPSHDTTLWL
                     LATDDERPVGALTANVWGERGWIDSLGVLAPYRGRGIGAALLRRSFATFADRGVGEVA
                     LSVDADNPTGATTLYERAGMRVVWRWDLWERPWVETPGPSSPANGPS"
     gene            complement(10878..11582)
                     /locus_tag="AC150V_12045"
     CDS             complement(10878..11582)
                     /locus_tag="AC150V_12045"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR3763693.1"
                     /translation="MRVLGVGAHPDDLEILCGGTLARYVQEGHEVVMCHGTKGNRGSF
                     EHTSEKIAEIRGREARRAAEICGAEYVSLEFDDAEVNAADRGQQIAVIDVIREARPDV
                     IITHFPDDYMGDHNEISRLVFDCSFYAALPLYETGKPYHTEIPPIFYMETIMGHGFQP
                     TEFVDVSGTIETKVAMLEAHQSQLTWLKQHNDVDIVEEMKTMTRFRGQQCAVEYAEGF
                     RPCLVHLRGRTTRVLP"
     gene            11708..12064
                     /locus_tag="AC150V_12050"
     CDS             11708..12064
                     /locus_tag="AC150V_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3763694.1"
                     /translation="MTSFDRSRFDHIGLITTEQMPGESWVEATRVWVTSPRDHPWNVE
                     FLRFEPDSPVTGPLRTEPHVAYRVDDVHEAIRGHDVLAEPFDVGDGFLTVAFVQYQGA
                     VIEFMQYSNPEEEGWF"
     gene            12055..12849
                     /locus_tag="AC150V_12055"
     CDS             12055..12849
                     /locus_tag="AC150V_12055"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763695.1"
                     /translation="MVLSENGRPKIEIRGATKIYEAKTGPVHALEGFTLDVRDGELVA
                     LCGLSGCGKTTLLWSVMGLHPLTAGEVLLDGRPVTGPRPDSIGIMFQEANLLPWRSVR
                     KNIEYPFQIRKQPVDARHIDSLLEEVGLVGFDASYPRELSGGMQQRAAIVRALAQDPE
                     VLLMDEPFGALDAFTRDEMNLLLMRLWSESSKTVVFVTHNISEAIFLSDRVVVMTPRP
                     GRLAKVFDIDLPRPRNVDMTFEPEFIELVKEIKAVVETGKPRVEVG"
     gene            12846..13718
                     /locus_tag="AC150V_12060"
     CDS             12846..13718
                     /locus_tag="AC150V_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR3763696.1"
                     /translation="MSDAPRELQDQIPSFEKDDAPAGGHGIELGGMTSVRGAKSALEI
                     GTIIAIGVIVFFAVEGLLRLANTPAYVFPKPGTVLTALGDDFWLQYGHHLSTTAFEFL
                     LGYLIGSTIGLVLAAVITQRPFVEKVVAPYVIILVVTPMLALVPFLRVSIIQGSSQWP
                     IVVAVALASGPMVLINSATGFRRTDLAKIALARSYGASTFQVFRTIRFPLALPMIIVG
                     YMVGAIFGLLTAIGGEMVSASEKGGLGRQLIYFSSLIQMEKFGAVILIIAALGVSIYV
                     VFYLIGKRWASWEA"
     gene            13763..14956
                     /locus_tag="AC150V_12065"
     CDS             13763..14956
                     /locus_tag="AC150V_12065"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR3763697.1"
                     /translation="MEDKIVIEELTSEGYDRREFLKRSAAASAAVAAVGTLGVEAASA
                     GSNQKLDTVRWVTPRGTLEVMDDYNIVVPMKMGWFKKLGINVKLFPGDASGNLPQVAA
                     NTYDMGYASPGVLTSSIDAGVPVTSIWSQIPGQVFDFVLPADSKITHPRQLKGKTISV
                     FNAGWIPIVNPMLVEVGVDPKTVKFREFGAQWNQAVALKLVDAGLSWEGLRAQLNGMS
                     AIFGAGGGFKFLVGQEWGSKLPSNSYQVRSKDLDDPKKVDIYTRFLTGVVMGFEFARY
                     NPRAAAQITYEQYPGLQKVITPQVAVDSLLELHKGYSASRRMAPGKYGFHYTKQWDKY
                     LGFIKDFDQTKKRLKVSEVLTNSFVNPANAKADRKTARREAVAYRKKLTGTFKNTTIP
                     KGTKL"
     gene            15026..15820
                     /locus_tag="AC150V_12070"
     CDS             15026..15820
                     /locus_tag="AC150V_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR3763698.1"
                     /translation="MANALAGHTNSYHTYGHDEALAGIAEAGYRYVELSAVPGWTEHV
                     DLSTPPAETRKKLEGYGLEAVSLSGHSDLTTPDGTEHGIAAVRWAADYGIPIVNTAVG
                     GHQSADENEAAFLANIGALADAADAAGVVVALEIHGDIMASSDVTIPLMEKIGRDSIK
                     VNYDTANVEYYSGEKAVDDLPKIIPYLAHVHLKETTGGKENWNFPAVGEGSVDFSRVL
                     EILSDAGYDGPYSVEIEFTGEPWPPLEEVNRSMRASREHLQTLGLS"
     gene            15849..16682
                     /locus_tag="AC150V_12075"
     CDS             15849..16682
                     /locus_tag="AC150V_12075"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR3763699.1"
                     /translation="MARSIFMPEMTNLEVRTYLEDGGTTVLVPVGSTEDHGDHGPLWT
                     DVYIPLEIAKRAAPDLDAIVGAPIPFGIAHDHRGAHGLVHLTMDTFFGLVRDVCRSLA
                     DAGFRKIVLLNGHYCNSHALEFAVARCHDELPEGTRVYPFPYWAGLAPEEAEQYLSGS
                     AGIHANVGETSIVLAINEELCDMENVRDFTPELPELKTNPLALLDPVFLATPGSFWSL
                     LEDGGGVWGSPSESTAEKGEEFLGWCARAVVNVVHDMDALHDQLEPRYLRSRRPQGAA
                     R"
     gene            16669..17463
                     /locus_tag="AC150V_12080"
     CDS             16669..17463
                     /locus_tag="AC150V_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3763700.1"
                     /translation="MPHAELDDRRVYYEEHGSGDPVLLVNGLGADHTTWGLQTEYLKE
                     SFRVVVFDNPGIGQTEGPSGPYTTELFGDVAAALLRYLGIERAHVVGASMGGMIAQQI
                     AARHPALVRSLVLHCSWWKADSYTQALIRSWQTLARATGILDLSRQIWLWVFAPGFYE
                     EQPDTFAELERQIRESPSQTVEEFCNQAEACLSHPTLEQVAQIEAPTLIGVGETDILT
                     PPAHSRALHERIPGSVLHIWPGMGHAPFWEIPEQFNRLNRDFLEAH"
     gene            17463..18461
                     /locus_tag="AC150V_12085"
     CDS             17463..18461
                     /locus_tag="AC150V_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR3763701.1"
                     /translation="MISLAILGGGFMGSAHAANFKALGDRVRVKTVASRTTERAAAIA
                     ETVGAEITDDLDAAIADPAVDVIDICLPTPLHREYTERAIAAGKHVFLEKPIALTLED
                     ADAIVSAAAGSDRVFMVGMVLRFWPEYVELQRLVAAGELGRPISVVASRLSPPADWND
                     WMADRSQSGGTPVDLMIHDIDQMNWLLGTPRTVYATEPTPGHVHAVVEYDGASGAAEG
                     SMCMPRSYPFSSLIRVLCEDGVAEYAFSAAPIEGEGNIGESSSARGLRLFPVDGDPRT
                     VAVESADPWGPELEEFVSCVEQGRQPEQGTGEQATLALRVALAASRSLESGVPEVV"
     gene            18461..19783
                     /locus_tag="AC150V_12090"
     CDS             18461..19783
                     /locus_tag="AC150V_12090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR3763702.1"
                     /translation="MNAAVERVGARINALRGEAVALLEELVRVNSVNPEFAGVQRADV
                     IGGETRVNEILRERYEQAGLETHWVAPDPERKNLVGVRKGSGGGRSVALNGHVDTVPP
                     VEPESWVNGSPWNPEIRDGRLYGIGSTDMKGSDAAMWLVAQALEDEGAELAGDLLLHS
                     VIGEETMQHDLGTSAVVEAGFGADAAIVTEPTSIPKPLTVSPIAAGNWYFRIHVEGRS
                     THCGNRALAIRPGGPGDAIGVNALEKAVRIVSSLQELERQWGLTKSHPYFMPGFFSLL
                     PGVFYSDPGLDIPFYFSNRAEIAYSLWYPPDETAEDARAEIEEYVLTGSRLDPWLRAH
                     PPRFEWINNWPAASTPWEDPIVQTMAHAHERSAGMTIPPPSPQHPVAFGAASDASFYE
                     AAGIPAVVFGPGDVRIAHCRDESVDLDEVVAAAHSLAACVIEWCDGRS"
     gene            19770..21476
                     /locus_tag="AC150V_12095"
     CDS             19770..21476
                     /locus_tag="AC150V_12095"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR3763703.1"
                     /translation="MAGARPWRAICQALAAENVEYVFGLPGNPELLYNDLYDFPQVKP
                     VLVRMETSAVFLAMAYARVSGRAGVVHASPGPGMANLVPGLLEAHFACSPLVCIVSGA
                     DRAHEGAGGFQDTPALEMVRPITKWTVRIDLPERTTWTMQRAFAVARNGKPGPVFVEV
                     PSDVAGMTAEIPDYRRLHSPLRARGDPAAIADAVRLLEDAERPVLWAGGGVGLSEAEA
                     ELLALAEALDAPVVTTPSGRGSISEEHRLGFGVVGLYRTESSARPVDEADAIVYVGTR
                     LEEFQSGLGRYLPPAAKSIQIDVDPFEIERGVTVDVGIAGDAKLVLRELADAVRPIEG
                     RAWTGDLAAFRADFEGRVEVECAPNGGALKTKQIVHGLNAVFDDGFILVNENGGQDLW
                     SYYCPYLKVTTHRGCVAPAEQTVMGLGVAGSVGAKLARPEAPVVCVTGDGAFQMYMKE
                     IPTAVQHGAPVLWVVLDNAGLHWVKWIAAATGEKYIAVDFEAQPDLVGVAQASGAHAE
                     RIEAPGELLDALARAKAAMEQGMPAVLVCGIDTWDYPQGFVDFHREMWGLELPARERS
                     TA"
     gene            21836..21979
                     /locus_tag="AC150V_12100"
                     /pseudo
     CDS             21836..21979
                     /locus_tag="AC150V_12100"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
     gene            complement(22086..22394)
                     /locus_tag="AC150V_12105"
     CDS             complement(22086..22394)
                     /locus_tag="AC150V_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763704.1"
                     /translation="MVARVARFEGVNFEGAVETMGEAEALIRPMMEGLAGYRGYAHLV
                     SREGEALAITFFDSAANAEAAEQTFDEEMPRKLGDLFERWEGRRASAGIYSVLADSRG
                     "
     gene            complement(22498..22785)
                     /locus_tag="AC150V_12110"
     CDS             complement(22498..22785)
                     /locus_tag="AC150V_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR3763705.1"
                     /translation="MHGYTHDKDALTKRLRRIEGQVRGLERMVEEDRYCIDILTQIAA
                     VETALEQVGAKLLEDHVNHCVAGALASGDDAEITEKTGELLDAVQRFVKTR"
     gene            complement(22785..24965)
                     /locus_tag="AC150V_12115"
     CDS             complement(22785..24965)
                     /locus_tag="AC150V_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016884789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR3763706.1"
                     /translation="MKTATPEAPERVQLAIEGMTCASCATRIERKLGKIEGVEATVNY
                     ATETAAVSYDPSAVSLDELVQSVESIGYRAALEGGAEEDTDALAVLRRKLVVAVALTA
                     PVVLLAMVPQVQFDGWEWAALALTTPVVFWSGLQFHRAALLNARHGAATMDTLISVGT
                     LAAWTWSTVVLLAGIDSHSYFEVAAVITTLILVGRFLETRARSRSGAAIRSLLELGAK
                     EARVLREGAEVLVPSDELQVGDVFVVRPGEKIATDGVVVEGFSAVDQSMLTGEPVPVE
                     VVASDEVAGATLNTSGRILVRATKVGADTALAQITRLVAEAQAGKAPIQRLVDRVSSV
                     FVPVVLAISLVTLAGWLAITGDASAGVTAAVAVLIIACPCALGLATPTALMVGTGRGA
                     QLGILIRGPETLERTRQISTIVLDKTGTITEGRMWVADLAPAPGVDRSELLALVGAAE
                     AASEHPIAQAIAAQALGELGEQADVESFTSRAGLGVEAIVEGHAVVVGRPVMLAAWGL
                     ELPTELAEEIGRAEAAGRTVVAAAIDGRPAGVFVVADRLKPTSSEAIEELKALGLTPV
                     LLTGDNDRAARAVGAEVGIENVLAEVLPHEKSNEITRLQRNGEVVAMVGDGVNDAPAL
                     AQSDLGLAIGTGTDVAIEASDITLVSGDLRGAADAIRLSRRTLRTIKGNLFWAFGYNV
                     AAIPLAVAGLLNPMIAAAAMAFSSVFVVTNSLRLRRFHSLREGT"
     gene            complement(24962..25162)
                     /locus_tag="AC150V_12120"
     CDS             complement(24962..25162)
                     /locus_tag="AC150V_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR3763707.1"
                     /translation="METLIYSVPAMSCEHCVAAVRGEISGLPGVSEVEVDLESKRVEV
                     HGPALDDAAIRAAIDEAGYEAT"
     gene            complement(25200..25607)
                     /locus_tag="AC150V_12125"
     CDS             complement(25200..25607)
                     /locus_tag="AC150V_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3763708.1"
                     /translation="MTGSTAPDSKPRRLISGHSPYEPIVGYSRAVVAGDRVYVAGTAP
                     IPQDGAPPPEDSYEQARLCLQIIEEALVRAGSGLEHVVRTRVFVTDVTHWDGVSRAHG
                     EVFGEIRPANTTIVAALLDPAWKVEIEVEAALP"
     gene            complement(25604..25987)
                     /locus_tag="AC150V_12130"
     CDS             complement(25604..25987)
                     /locus_tag="AC150V_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006090280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR3763709.1"
                     /translation="MERRLVASGSPYEPIMGFSRAVRVGPHVHVAGTGPNMEDGGEPP
                     TDAYGQTKRCPEIVLAALEEAGARPEDVVRTRIYATSADDFQDVARAHGEVFAEVRPA
                     NAFMVVSGFADARWKVELEAEAIVP"
     gene            26007..26813
                     /locus_tag="AC150V_12135"
     CDS             26007..26813
                     /locus_tag="AC150V_12135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR3763710.1"
                     /translation="MDALRQVDDWDVGFAAAGVTRVGGVVAAHGGRRQMVRLASVSKV
                     VTALATLVAAEEGVIDLDEPAGPPGATVRHLLAHASGLPFEGHVVLGSPARGRIYSNE
                     GFRVLGEHLARRAEMPVAEYIRAAVVVPLGLELDPTGHPGAGMHGHLDDVLALGREFL
                     APTLVAPETFAEATQVQFPGLAGVLPGFGRQATNDWGLGFDLKDAKSPHWTGERNSPL
                     TFGHFGGSGTFLWVDPSVGVSCAALTERPFGDWAKAAWPALSDAVLAELA"
     gene            complement(26810..27538)
                     /locus_tag="AC150V_12140"
     CDS             complement(26810..27538)
                     /locus_tag="AC150V_12140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR3763711.1"
                     /translation="MRSVLLVHAGIADARMWRPQITALEAAGFRAVAPDLRGFGTRAL
                     EAGRFSHVDDVRAALGGCPAAVVGSSFGGRVALELALTAPSLVERLVLIAPGLDGWEW
                     SAEVTSGWEEEEAALERGDLGAAADSNLRLWLDRGQDVDPGVRSLVREMVLRSFELQL
                     PVGDAAEDTWPASGASERLDRLATPTLVLIGEMDTLDMQSIAARIAAEAQNATLRRIP
                     GAAHLPSLERPDLVNRLLLDFLEV"
     gene            complement(27535..>27962)
                     /locus_tag="AC150V_12145"
     CDS             complement(27535..>27962)
                     /locus_tag="AC150V_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR3763712.1"
                     /translation="RGGSPSPLPYPAVSPQHVPASITELSRVGLLAELPGEQLTRLAG
                     RMRREDVAPGTAIISEGEPGDRFFVLLSGVAAVSQTDRGARGMIRPGEYFGEVAPALG
                     VARTATVTAMTPCVIASCDRETFEELILPLFSDRGGDAA"
BASE COUNT         4385 a         9557 c         9762 g         4258 t
ORIGIN      
        1 cagggcggcg ccacgcgatc accagtgcgg gcgcggcgac gagcgcggtc gcgccgatcg
       61 cgaccgctgc aacgccgatc tgggtcagcg cgacccctcc gagcagtgcc aatgcggcgc
      121 cgagcagtcc cgccacgaga tccgtgaaac ccacgagtcg gccgcgctcg gacggggccg
      181 cccgcgtcac gagctcggcg gccgacgcga cgtacgagag gctccagccg agtccgagtc
      241 cgaacaacga gaccgacatc cagaggatgc ttccggccca ccacagcggc agcgtcgaag
      301 ccgccatcag caccaggccg gccacggcgg ccgtacggcg accgatgcgc tcaacgaggg
      361 cgccgacgaa caggacgagg gcgtacatcc cgacgatgtg cacgctgatc acggtgaaca
      421 cgtccgcctg gggatggtcg ttgccgacga tgatgtagcc ggtcaggttc atgacgctga
      481 ccatgacggc gaagctcgcg accgtggcgg cgagtgccac gaggacgccg ggccggcgca
      541 ggatctcggt gagaggggcc gtcgagacgc cgccgctctc ccccgtgtcg agtgcgatcg
      601 cgatcgtccg agggtcgggt cgcacggcga gcgcgagcgc caggccgacg agactgatcg
      661 cgcccgctgc gagccacgga acgatcaggc tgtcgagctc tatttccttg ccggcgaaga
      721 gcggccgaaa gacgagtggg ccgagcagtg cgccgaagag ggccccgaac aggacgagcg
      781 agatcgcatg tgcctttcgc ggcggcggca ccatatcggc cgcggcggcg cgggagagca
      841 ggatcgtccc gctggtgatg ccgactccga cgagtccgag gacgaccaac gcggcggaag
      901 ccgtcgcaca gccgagcgcc gtcacggcgc agctggcggc gccgaccagg aagccggtcg
      961 cgatcacggg catgcgcccg acgcgatcca tcgcccgccc cgcgggaaac gcggccagcg
     1021 cgcccgcagc cagaaagatc gcgggtccta gaccgaggat cgactccacg cccgtgacga
     1081 ggacgagcgt gatcgtggcg actgccgcgg ctagctgcaa catccccgag aggctcgtca
     1141 ggcaggccgc gagcagcgcg atgtttcggc ccaccgggaa gctctgctcg tccggggatg
     1201 ccggaagctg cgccgtttgc gagactcttc cggccatggc ctgcattctg ctcgacatcg
     1261 acggtgtgct gcacgtctcg ggtgaagcca ttcccggctc ggttgacgcg gtggccgagc
     1321 tacggaaaag cggccactcg ctccgtttcg tgacgaacaa ctcgacgctg ccgcgggcgg
     1381 cgctcgcgga cgagcttcgc agtctcggct tcgagctcga cgaggaggag atccagacga
     1441 caccgcgcac cgccgccggc gagcttgcgg ggaagcgggt cttcgccctt gtgatgcctc
     1501 cggtcgtagc cgatctcgaa gggctcgagc tgtccgcgat ggacgtcgaa gcggtgctga
     1561 tcggcggctg tgacgagacg ctcgagccga accaggtctt tacgtacatg aatcttgcgc
     1621 gggcgttcgc cgagatccag gcgggggccg agttctactg cctgcacaag aacaagtggt
     1681 ggcagacccg gcgcggcccg atgctcgacg ccggagcctt cgtcgcgggg ctcgagtacg
     1741 ccaccggcgt cgaggcgacc gtcgtcggca agccctctcc cgcgtacttc gcttccgctc
     1801 tcgacgcgct ggaggcggaa cccgagctga cgtggatggt caccgacgac atcgagcagg
     1861 acgtgcgcgg cgcgcagctc ttcgggatgc gaaccgtgct gttgcgcacg ggaaagttcc
     1921 ggccggagac gctcgaagcc tccggcgtga cgccggacgt cgtgctcagc tcgctcgcgc
     1981 agtttcccga ctggctcgaa cggcacctct gatgacggct ccgggcgcgc ttcgcgtcgg
     2041 gatggacctg atcgagatcg agcggatccg gagagcgttg gatcggcccg gctttcggca
     2101 gcggtgcttc acggaggccg agcaggccta ctgcgaatcg aagcgcaact ccgccgagag
     2161 ctacgcggcc cgattcgcgg gcaaggaagc ggtcggcaag gccctcggct gcggcgtgta
     2221 cttcacgtgg aaggagatcg aaatcgcggg tcgccccaag ccgggcgtgc gtctcttcgg
     2281 gaaaaccgcc gcgtgggccg agaaggtcga ggccggccga atcgacctct cgatgacgca
     2341 ctcgcgtgag ctggcggccg cgatcgcagt cgtccaggag aaggcgtgat tctcgagccc
     2401 ctctatacgg ctgccgagat gcgcgcggcc gaagagcgct accccgacta cccggagtcg
     2461 atcccggagt tgatggagcg cgccggacgg gcgctcgcgg aagagtcgat gcacgccttc
     2521 cccggtgttc ggcgcatcgc ggtcgtctgc ggcggcggct ccaatggggg cgacggccgt
     2581 gttgcggcgc gagttctgcg cgaggcgggc ctggaggccg aggagacgga cgtgctcgac
     2641 ggcgcagatg tcgtgatcga tgccctcttc ggaaccggtt tccgcggagc gccgaggccg
     2701 gaagcggcgg cgttgatcga tcggatcaac tcggccggcg ttcctgtcgt cgcctgcgat
     2761 ctgccttcgg gcgttaacgc gacgaccggc gaggtggagg gcgccgccgt gcgcgcttcg
     2821 ctcacggtga gcttccatgc gcccaaggtc ggtctcgtcg tcgctcccgg ccgccgccac
     2881 gcgggccggg tcgtcgtcgc cgacatcggc ctcgaggaca cctcgaccgc ggcgaccaga
     2941 gcgacccccg aggtgctctc gctcgtgccg cgtcgcagcg ccgtcgattc caagtactcg
     3001 gccggaaccg tgctcgtcgt gggtggttcg ccggggatga ccggtgcagc cgccctggcg
     3061 gcgaccgcgg cactccgtgc cgatgccggc tacgtgattc tggcagtgcc cgccgtctcg
     3121 ctcgcggtgc tggagatcct cacgctcgag ccggtcaaga tcggattcga ctgggaatca
     3181 ggcgtcgagg cggtgctcgc tgccgcagag cgcgccggcg ccgtcgccct cggacccggc
     3241 ctcggccgct ccggcgaggc acgcgggctc gtgcgggcgc tgctcgagcg gctcgagcga
     3301 ccggtcgtcg tggacgccga cgcgctctac cgcctcgagc ccctcgagcg cgccgcgccg
     3361 acggtgctga cgccccacgc gggcgagctg gcccggttgc tcgagacgac gtcgtcggag
     3421 gtcgatgctg cgcggctcga cgccgcgcgg caggcagcgg agcgcttcgg agccgtctgc
     3481 ctcctgaagg gagccgacac gatcgtggcc tcgcccgatg cgggcccgat cgtctgcgat
     3541 cacgggccgc cttcgctcgc gacggccggg acgggtgacg tcctgaccgg ggtggttgcc
     3601 gcgttcctcg ccaagggcgt cgagcccagg ttggcggcgg cggccgccgc gaccgcgcac
     3661 ggtctcgcgg ccgcctccat ccagcatcag gcgggagtcg tcgcgggcga cgtcgcagcg
     3721 gcgttgccgg cagtgctctc ctgacgtcgt gcgcgtcgag attctcggct cgggtggagc
     3781 gaccacgaca ccgcggccgg gttgcacctg ccgggtctgt gtcgaggcgc ggaagcaggg
     3841 cccgccgcac tcacgcaccg gcccgagcgc gttcgtgcat gggcccaacg tgctcttcga
     3901 cacgcccgag gagtcgaagc tgcagctcga ccgcgccgga ataggcgaga tctcggcctg
     3961 cttctactcg cactggcatc ccgaccacac catgggccgg cgcgtcttcg aaacgcgcaa
     4021 cggcgatttt cgcgcctggc ctcccgaggc gaagcgtcag ctgacgaccg acgtctacct
     4081 gccgcagcag gtggcagccg atttccggga gcgtctcgga gcgatggacc atctcgagtt
     4141 catgcaggag cgcggctggg tgcggatccg cgagctgagc gacggcgaca cggtcacgat
     4201 cggcggcgtc gagatccgac cgttccgggt cgccgaggac tacgtctacg ccttcgagct
     4261 acgaggcggc gggcgccggc tgctgctcgc gatggacgag ctcaacaact ggacgccgcc
     4321 gcgcgcggtg aggggatgcg acctcgccgt gatcccgatg ggaatctgcg agttcgatcc
     4381 gctcaccgga cagcggcgca tagctccgga gcatcccatt ctccgcttcg aggcaacgca
     4441 cgacgagacg ctcgagatcg tcgacttgct ccgcgccgac cgggtcgttc tcagccacat
     4501 cgaggagata gacggtctca cggtcgacga cctgcacgac ctggagaccc gcctacgcga
     4561 cggggggcgc gcggtgacgt tcgcgtacga cgggttgatc gtcgatctct agaaacggcc
     4621 gttccaacga cggcgtagcg gagaatagag acgtggcccg ctctgcgttc acgatcgacc
     4681 tcggcgccgt acgccacaac gtgcgggcac tcctcgacat cctcgacggg gcggagctct
     4741 gggccgtggt gaaggccgaa gggtacgggc acggggcgat cgacgtcggc agggcggcgt
     4801 tgcaggcggg cgcgcacgca ctctgcgtcg ccaccgtgcc cgaggcagtc gccctgcgcg
     4861 cttctcttcg cgaacctcgc attctcgtca tgggcccgag cacctcggcc gaggtcgcgc
     4921 aggcgaggga ggcgcgcctg gagctcgcgg tggtcggcgg gccgctgccg gagggggttc
     4981 ccgttcacgt caagctcgac accggcatgg gacggtgggg cctcgccgag ctgcccgcgc
     5041 tggggccaca ggtcgtcggg ctgatgagcc acttcgcctc ggcggactcg gatgcggcgt
     5101 tctcccgtcg ccagctcgag cggtttgccg ccgccactgc atcgcatgcg cggcttaccc
     5161 ggcacatcgc caatagcgcc ggtgcactca cactccccga cgccaggctc gatgccgttc
     5221 ggtgcggcat cgccctttac ggcgtctctc ccttcggagg cgatccggcc gatcacggcc
     5281 tccggccggt gatgcggtgg gagtccgagc tcgcgcacac gcgcctcctc gcagcgggag
     5341 agagcaccgg ctacggccgc acctttgtcg cgcgacggcc gacatggatc ggactcgtcc
     5401 cggtcggcta tgcggacggg ttccggcgcg acatgaccgg aaccgaggtg agcgtcggcg
     5461 gcgagcgttg cccggtggtc ggaaccgtgt cgatggacgt ggtcgcggtc gagctgcccg
     5521 ccgagcgacc cgcgggcacg gtcgtgacgt tgctcggcga cggaatcacg gctgagcgcc
     5581 atgcagccgt ggcagacacg atcggatacg agatcctgtg tggtatgaac gcgaaggccc
     5641 cacgcgcgac cagggaggtc gtaggtgagt gatcacgtcc aacggcggtt ggccctccag
     5701 gagcgacggg ccgaggcgct tgcagcaagg accgagcgga tgctcgcccc gttcaccggg
     5761 cgagagcgcg cgctcgatgc cggctgcggt gccggcgcgc tctcgttcgc catcgccccg
     5821 cacgtcgccg aggtcgtcgg gatcgaccgc gacgagggcc tggtcgcagc cgcccgcgaa
     5881 cgagcaccgg ccaactgcac gttcgtggac ggcgatctcc tttcgctccc gttcgagtgg
     5941 ggtgagttcg acctgtccgg ctgtgcgcgc gttctgcatc acgttaggcg gcctgagctc
     6001 gtgatggcgg agatagcgcg agtgactcgc ccgggcggtc ggatcctgat cgttgaccag
     6061 ctcgcctccg tcgatccggt agccgcgatc gagcacgacc gattcgagcg tgcccgcgac
     6121 cggtcgcaca accgcctgct gcccgacacc gatatccggt cgctcttgga catgaacgac
     6181 ctcgtggtgc tggagaacga ggtcgagcac gagcagcgtg acgtcgactc gtacctcgag
     6241 ctcgcgggga tacccgagga tcagcgggag cgacttcgcc ggatggcgcc ggcagacgtc
     6301 tacgacgtcg agatcggctg gtacgtcgcg cgcaagcgcg gctaggtatc cgcgtctctc
     6361 cccaccgtgg cgtcccgccg ggaacgattg tcaccatttc ggcacgtgtg ggccgcggcc
     6421 aagagggcca tacgagggga cagtcccccg gacttggctc gggaaggccc tcggggagca
     6481 attgtcgctc tttcgacaca gaccacgtgt ccacttgcgg ccgggttctc cgcgcgtctg
     6541 gggtcacggc cagcgcacgt gagcgcccgc ggcggtggcc ctagcggggt tcgtcgcccg
     6601 ggtatcgggc cacggcgagg acgcgcgtcg tcagggtcgg gaagagagcc tgagcgacgg
     6661 cgcccagccg gtacgggaac caggggacga ccacctccgc acggccccgc tccacggcgg
     6721 agacgattgc gcgcgcagtc tcctccggcc ccacgacgaa gcgccgcccg agtcgcgttc
     6781 ggaagagcga cgggtgcggg aagcccggcg tcgtcacgaa gcccggcatc acggtgtgaa
     6841 cgtcgatccc gcttcccgcg agcactgcgc gcagcgagcg cgagaacgcg acctgcgcgt
     6901 gcttcgacgc cgagtacgca ccggccggcg cgaaggcaac cgtgccgccg atcgaggcga
     6961 cgttgacgat gtgtgcgtca ccgtctgccg ccgaggctcg caggccgggg aggaacgcgc
     7021 gggttgccca cacaccgccg aggtagttga cctgcatcgc gctctcgacg agatcgagat
     7081 cgacctccgc gaagctgccc cgcacgagga agcccgcgtt gttgacgagg agatgcagcg
     7141 ccgggtggcg gagacggatt cgctccgctg cagcgtcgac ggccgcacgg tcggagatgt
     7201 cgcagacctc tacctcgcca ccgatctccg ctgcgagagc gctgagcggc tcctggcgtc
     7261 gagccatcag caggcagtgc cagccacgag tggagagcag gcgggcggtc gccgcgccga
     7321 tgcccgagga ggcgccggtc acgacggcca cccggcgggt cggcgtggcg ctcacgaggc
     7381 ggcagcctac agcggtcggg atggccctat cgtgcggcgt gccatcctgg ccgccacggc
     7441 gggcaggatg gcctgaacga gcccggccag ccggtagggg aaccagggga cgaccacctc
     7501 ggcgcgtccg gcttccactg cgcggacgat tgcacgtgcg acgtgcggag gccggacgac
     7561 gatccggcgt ccgagctgat gagccagcat ggcccgctgc ggaaaccctt cggtctcgac
     7621 gaagccgggc aacaccgtgt gcacctcgat tccctccggc cagagcgtcg agcgcagcgc
     7681 ccgggagaag gcgacctgcg cgtgcttgga cgcggcgtac gctccggctt gggcgaacgc
     7741 catcgtgccc gcaaccgacg cgacgttgac gacgtgcgcg ctcccggttg ccgcagccgt
     7801 acgcaggctc ggcagcaaca gtcgggtcaa gcgcacaccg ctcgagaagt tgacggccag
     7861 cacccgctcg atcgcctctg acgggaggtc gagcaccgtt ccccgcgccg ggatacccgc
     7921 gttgttgacg agcaggtgca gcgacgggtg gcggtggacg agagaggcag ccagcgccgc
     7981 cagtgcgtcg cggtcggcca ggtcgcacgg tgccgcctcg ccttcgatct cggccgccag
     8041 cgcccgaagc cgctcttcgc gccgggcgac gagcacgcac cgccaacccc tgcgtgccag
     8101 ctcgcgggcg gtcgcctcgc cgatccccga cgaggccccg gtcacaacgg ctatgcgctg
     8161 aacgtccgca ccgtcccgag gttgatctcg accgtgtcac ggatgtcctc ggcgtaccct
     8221 ccggccagcg tgacgcagat cggaaccccc gccgcgcgaa gcgcgtcgcg cacgagctcg
     8281 tcccggcggc gcagcccctc gatcgagagc gccaggcgtc cgagacggtc acccgcgtag
     8341 ggatctgctc ccgccaggta gaagcagagc tcgggccgcg atcgagcgag cgcctgcggt
     8401 acgagcgctc cgagcgcctc gaggtatgcg tcgtcgcctg tcccgtccac gagctcgacc
     8461 tccagatcgc ccggtacccg cttgaacggg tagttcctga agccgttgac cgagcacgtg
     8521 aacgcatcgg cgtctgcggc cagcagcgcg tgcgtgccgt cgccctggtg gacgtcgaga
     8581 tcgagcacca gcacacgtcg cacacgaccc gtgagcgccc gccgagccgt cacgacgtcg
     8641 ttgaacacgc agtagcctcg cccggcgtcg gcgaacgcat gatgcgtccc gccgccgagg
     8701 ttcatggcaa cgccgtccgc cagggccgcc tccgcagcct gaaccgtagc gccggtcgac
     8761 ctcttcgccc gctcgacgag ctcaggcgac cacggcagtc cgagcgcgag ctcctcgcgg
     8821 cgcgagagcc ccccgcgcgc gacgcggccc agatagtggt cgtcgtggac gcgctcgagc
     8881 tcgtcgtcga cggctgccct tgcagcgtga ccctcgacgc cggagagtcg ctcggccctc
     8941 tccctcacga gccggtactt cccgagcggg aatcggtggc cctcgggcag gggataggtg
     9001 aacaggtcgt gcgcgtacgc ccgcacagcg cggccaacct accgtaggat cgcgccgtgg
     9061 ccgggagact tggcgggaag caggcgctcg tgctcggcgt cgccaacaag cggtcgatcg
     9121 cgtgggcgat tgcccgccgc ctccacgaag agggcgcaga gctcgcgttc acctatcaag
     9181 gcgagcgaat cgagaaaacg gttcgcgatc tcgcagccac cgtcgacggc aagctggtca
     9241 ccgagtgcga cgtccgctcc gacgacgaca tcgagcgggt cttcgccgag acggccgacg
     9301 tcttcggcgg cggactcgac gtgctcgtgc actcggtcgc cttcgccgcc gcggaggatc
     9361 tcgagggccg cttcgccgac actccgcgcg accgcttctg gatggccctc gacatcagcg
     9421 cctactcgct cgtcgcatgc gcccgtgccg ccgagcctct gatggaggcg cggggcggcg
     9481 gttcgatcct cacgatgacc tatctcggcg gcgagcgcgc cgtgccccac tacaacgtga
     9541 tgggggtcgc caaggcgacc ctcgactcct ccgtgcggta cctcgcatgg gatctcggca
     9601 agaagggcat ccgcgtcaac gccgtctcgg cggggccggt gcgcaccctc gctgcccgct
     9661 cgatcgccgg cttcacgacg atggagacga tgttcgagga acgggcgccg ctgcaccgcc
     9721 acatcgacgc ggacgacgtc ggcgctgcgg ctgcgtacct gttgtcggag gatgcccgga
     9781 acgtcagcgg gaccacgctg tacgtcgact ccggctacca cgcgatgggc atgtagggga
     9841 tcagcgcccg ccgtctcctg cttggaggct aggaggggcc gttcgccggc gaggaaggcc
     9901 caggggtctc gacccacggg cgctcccaga ggtcccagcg ccagacgact cgcattcctg
     9961 cacgctcgta cagcgtcgtg gcgccggtcg ggttgtccgc gtcgacgctc agggcgacct
    10021 cgccgacgcc gcgatcggcg aacgtcgcga acgagcgtcg gagaagtgcc gccccgatcc
    10081 cgcgaccgcg atacggggcc agaacgccca gggagtcgat ccagccgcgc tctccccaga
    10141 cgttcgcggt cagggcaccc accggccgct cgtcgtccgt cgcgagcagc cagagcgtgg
    10201 tgtcgtggct cgggctcgcc gtctgcgtct cggtccagcg atcgaacggc tccggctcgt
    10261 ggccccagtg gtcggcgaac gcgtcgtcga ggactgcgtg aacggcccga agatcgccgg
    10321 gcgagctgat gccggcgatc aggatgcctt ccgctgccgg ttcgggctcg gacgcctcct
    10381 gcaggtcgat ctgcaggtgc cagaagtggc gtatcgggcg caggccccga gcggcgagca
    10441 acgccgccgc agtcgggtcg ctcgcgttga ccgcatggcg gaagcgggcg gaacggagcc
    10501 cggccagctg ctgcgacgca cgcatctcga tccagtgcag cacggcggag ccgatgcccc
    10561 gcccgcggtg ctcggggtgc acgacacccc aggacacaac cagggcccgc tccgcgagcg
    10621 ctgcctgccc gtagccgacg atcgtcccgt tcgtgtcggt gacgacccag gcgtccacgg
    10681 cgaggtcgaa gcccacccgg ctccactcgt cgtggacaaa gtgctcgtcg aggacgatct
    10741 ttccgctgtc gtcgacgtcg tcggcctgca ggacctcggc gacagccacg aggtcgtccg
    10801 aggtcggaac ccgtcctcgg tagccgtccg ggaggttcac gccgggaacg tacccgcggc
    10861 cgggctttgc cgagggatca gggaagcacg cgcgtggtgc gtccacgcag gtgcacgagg
    10921 cacggccgga acccctccgc atattcgacc gcgcactgct ggccgcggaa gcgcgtcatc
    10981 gtcttcatct cctcgacgat gtctacgtcg ttgtgctgct tgagccacgt gagctggctc
    11041 tgatgcgcct cgagcatcgc caccttggtc tcgatcgtgc ccgacacgtc gacgaactcg
    11101 gtcggctgga agccgtgccc catgatcgtc tccatgtaga agatcggcgg gatctccgtg
    11161 tggtacggct tgccggtctc gtacagcggc agggcggcgt agaagctgca gtcgaagacg
    11221 aggcgggaga tctcgttgtg gtcgcccatg tagtcgtcgg ggaagtgggt gatgatcacg
    11281 tcgggccgcg cctcgcggat gacgtcgatc acggcgatct gctggccgcg gtcggcggcg
    11341 ttgacctcgg cgtcgtcgaa ttcgagcgag acgtactcgg cgccgcagat ctcggctgcc
    11401 ctcctcgcct ctcgacctct gatctcggcg atcttctccg aggtgtgctc gaagctgcct
    11461 cgattgcctt tcgtgccgtg acacatgacg acctcgtggc cctcctgcac gtagcgggcg
    11521 agcgttccgc cgcagaggat ctccaggtcg tccgggtggg cgccgacgcc gagaactcgc
    11581 atcaggcgca gaccataacc gcgtctggcg cgcagatgtc cattgacgcg aggtctgggg
    11641 caatgctaag aagctttctt accggtagac cagggaatcc cgaaatcgac atgagcacag
    11701 ccacgacgtg acgtccttcg accggtctcg gttcgaccat atcggcctga tcaccaccga
    11761 gcagatgccc ggggagagct gggtcgaagc gacccgcgta tgggtgacga gcccgcgcga
    11821 ccatccgtgg aacgtcgagt tcctgcgctt cgagcccgac agccccgtca cgggcccatt
    11881 gcgcaccgag ccgcacgtcg cctatcgcgt cgacgacgtg cacgaggcga tccgcggcca
    11941 cgacgtactc gccgagccgt tcgatgtcgg cgacggcttc ctgacggtcg ccttcgttca
    12001 gtaccaggga gcggtgatcg agttcatgca atactcgaac cccgaggaag agggttggtt
    12061 ctgagcgaga acggtcgccc gaagatcgag attcgaggcg cgacgaagat ctacgaggcc
    12121 aagaccggcc cggtgcacgc gctggaaggg ttcacgctcg acgtgcgcga cggcgagctc
    12181 gtcgctctct gcgggctgag cggctgcggg aagaccacgt tgctctggtc ggtgatgggg
    12241 ctgcacccgt tgaccgcggg cgaggtcctg ctcgacgggc gaccggttac cggtccgcgc
    12301 cccgactcga tcgggatcat gttccaggag gcaaacctcc tgccgtggcg gagcgtccgc
    12361 aagaacatcg agtacccctt ccagatcagg aagcagcccg tcgacgcgcg gcacatcgac
    12421 tctctcctcg aggaggtcgg gctggtcggc ttcgacgcct cctatccccg cgagctctcc
    12481 gggggaatgc aacagcgcgc ggcgatcgtg cgggcgctcg cccaggatcc cgaggtcttg
    12541 ctgatggacg agccgttcgg cgcgctggac gccttcaccc gtgacgagat gaacctcctg
    12601 ctgatgcgcc tctggtctga gagcagcaag acggtcgtgt tcgtcacgca caacatcagc
    12661 gaggcgatct tcctctccga ccgcgtcgtc gtgatgacgc cgcgtcctgg acggctggcc
    12721 aaagtcttcg acatcgacct cccgcgcccc cgcaacgtcg acatgacgtt cgagcccgag
    12781 ttcattgagc tcgtcaagga gatcaaggcg gttgtcgaga ccggcaagcc gagagtggaa
    12841 gtcggatgag cgacgcgccg cgagagctcc aggaccagat cccgagcttc gagaaggacg
    12901 acgcaccggc gggcgggcac gggatcgagc tcggcggcat gacctctgtc cgcggcgcga
    12961 agagcgcgct cgagatcggg acgatcatcg ccattggggt gatcgtgttc ttcgctgtcg
    13021 aaggtcttct gcggctcgcg aacacgcccg catacgtgtt cccgaagccc ggcacggtcc
    13081 tgaccgcgct cggcgacgac ttctggctcc aatacgggca tcacctctcg acgacggcct
    13141 tcgagttcct gctcggctac ctgatcggct cgacgatcgg actcgtgctc gcagccgtga
    13201 ttacgcagag gccgttcgtg gagaaggtgg tcgctccgta cgtgatcatc ctcgtcgtca
    13261 cgccgatgct ggcgctcgtc cccttcctga gggtgagcat catccaaggg agcagtcagt
    13321 ggccgatcgt cgtggcggtg gcgctcgcgt ccgggccgat ggtgctgatc aactcggcga
    13381 cgggctttcg gcggaccgac ctcgccaaga tcgcattggc acgctcatac ggtgccagca
    13441 cgttccaggt gttccgcacg atccgcttcc cgctcgcgct gccgatgatc atcgtcggct
    13501 acatggtcgg tgccatcttc ggcctgctga cggcgatcgg cggggagatg gtgtcggcca
    13561 gcgagaaggg cgggctcggc cgccagctca tctacttctc gtcgctgatc cagatggaga
    13621 agttcggcgc ggtgatcctg atcatcgccg cacttggagt ttccatctat gtcgtgttct
    13681 acttgatcgg caagcgctgg gcgagctggg aggcttagcg gatttgaccc cggacgacgt
    13741 caacgcaggg aagggaggaa ggatggagga caagatcgtc atcgaagagc tcacttccga
    13801 gggctacgat cgtcgagagt tcctcaagcg cagcgccgcg gcaagcgcag cagtggccgc
    13861 ggtcgggacg ctcggcgtcg aggcggcgtc ggccggctcg aaccagaagc tcgacacggt
    13921 tcggtgggtc acgccgcgcg gcacgctcga ggtgatggac gactacaaca tcgtcgtccc
    13981 gatgaagatg gggtggttca agaagctcgg gatcaatgtc aagctgttcc cgggcgacgc
    14041 atcggggaac ctgccgcagg tcgcggccaa cacctacgac atgggctacg cctccccggg
    14101 cgttctgacg tcctcgatcg acgcgggcgt tccggtcacg tcgatctggt cacagatccc
    14161 cgggcaggtg ttcgacttcg tcctgccggc ggacagcaag atcacgcacc cgcggcaact
    14221 gaagggcaag acgatctccg tcttcaacgc cgggtggatt ccgatcgtca acccgatgct
    14281 ggtcgaggtc ggcgtcgatc cgaagacggt caagttccgc gagttcggtg cccagtggaa
    14341 ccaggccgtc gcgctcaagc tggtcgacgc cgggctctcc tgggaggggc tgcgggcgca
    14401 gctcaacggg atgtccgcca tcttcggcgc ggggggaggc ttcaagttcc tcgtcggtca
    14461 ggagtggggc tcgaagctgc cctcgaacag ctaccaggtg cggtcgaagg atctcgacga
    14521 ccccaagaag gtcgacatct acacgcgctt cctcacgggc gtcgtgatgg gcttcgagtt
    14581 cgcccgctac aacccgcggg cggcggcgca gatcacctac gagcagtacc cggggctgca
    14641 gaaggtcatc acgccgcagg tggcggtgga ctcgctgctc gagctccaca aggggtacag
    14701 cgcctcgcgg cggatggcgc cgggcaagta cggcttccac tacacgaagc agtgggacaa
    14761 gtacctcggc ttcatcaagg acttcgacca gacgaagaag cggctcaagg tgagcgaggt
    14821 cctgacgaac agcttcgtca acccggccaa cgccaaggcc gacaggaaga cggcgcgccg
    14881 ggaagcagtg gcctacagga agaagctaac cgggaccttc aagaacacga cgatcccgaa
    14941 gggcacgaag ctctagaagc actgccacga atcggtgtcg aggcgccccg ctccaggggc
    15001 gcctcgcgcc tgaagggagg acgagatggc gaatgcgcta gccgggcaca cgaactcgta
    15061 ccacacctat ggccacgacg aggccctcgc cggcatcgcc gaagcggggt accgctacgt
    15121 cgagctttca gccgtccccg gctggaccga gcacgtcgac ctgtcgactc ctcccgccga
    15181 gacgcgcaag aagctggagg gctacgggct cgaggccgtc agcctctccg ggcactccga
    15241 cctgacgact cccgacggta ccgagcacgg gatcgcagcg gtgcgctggg ccgccgacta
    15301 cgggattccg atcgtcaaca cggccgtggg tggccatcag tccgcagacg agaacgaggc
    15361 ggcgttcctc gccaacatcg gtgcgctcgc cgacgcggcc gacgccgccg gagtcgtcgt
    15421 cgcgctcgag atccacggtg acatcatggc gtcgagcgac gtcacgatcc cgctgatgga
    15481 gaagatcggc cgcgactcga tcaaggtcaa ctacgacacc gccaacgtcg agtactactc
    15541 gggggagaag gcggtcgacg atctacccaa gatcattccc tatctcgccc acgtccacct
    15601 caaggagacg acgggcggca aggagaactg gaacttcccc gccgtcggtg agggatcggt
    15661 cgacttcagc cgcgtgctcg agatcctgag cgacgccgga tacgacggcc cgtactcggt
    15721 cgagatcgag ttcacgggag agccgtggcc gccgctcgag gaggtcaacc gcagcatgag
    15781 ggcctcccgc gagcatctgc agacgctcgg cctttcctga gaaaaaacgg cgcaagagag
    15841 gaggcggcat ggctcgctcg atcttcatgc ccgagatgac gaacctcgag gtgaggacgt
    15901 acctcgagga cggtgggacg acggttctcg tcccggtcgg ctcgaccgag gaccacggtg
    15961 accacggccc gctctggacg gacgtctaca tcccgctcga gatcgccaag cgggcagcgc
    16021 ccgacctcga cgcgatcgtc ggcgcgccga tccccttcgg gatcgcccac gaccaccgcg
    16081 gcgcacacgg gctcgtccac ctgacgatgg acaccttctt cggcctggtg cgggacgtgt
    16141 gccgttcgct cgcggatgcc ggcttccgca agatcgtcct gctgaacggc cactactgca
    16201 actcgcacgc gctggagttc gccgtggcgc gctgccacga cgagcttccg gaggggacgc
    16261 gggtataccc attcccctac tgggcggggc tcgcgccgga ggaggcggag cagtacctgt
    16321 cgggaagcgc cggcatccac gccaacgtcg gcgagacgtc gatcgtgctc gcgatcaacg
    16381 aagagctctg cgacatggag aacgtgcgcg acttcacgcc ggagctcccg gagctcaaga
    16441 cgaacccgct cgcgctgctc gatccggtgt tcctcgcgac accgggatcc ttctggtcgc
    16501 tgctcgagga cggcggtggg gtgtggggca gcccgagcga gtcgacggcg gagaagggcg
    16561 aggagttcct cggctggtgc gctcgcgccg tcgtgaacgt cgtccacgac atggacgcgc
    16621 tgcacgacca gctcgagccg agatatctcc ggagtcgaag gccccagggt gccgcacgct
    16681 gagctcgacg accgccgcgt ctactacgag gagcacggct cgggcgatcc cgtcctgctc
    16741 gtcaacggcc tcggcgccga tcacacgacc tggggattgc agaccgagta cctgaaggag
    16801 tccttccggg tggtcgtctt cgacaatccc ggcatcgggc aaacggaagg gccgtcggga
    16861 ccgtatacga cggagctctt cggcgacgtc gccgccgcgc tgctccgcta cctcggaatc
    16921 gagcgcgccc acgtcgtcgg tgcatcgatg ggagggatga tcgcgcagca gatcgccgcg
    16981 cgtcatcccg cgctcgtccg ctcgctcgtt ctgcactgct cctggtggaa agcggattcg
    17041 tacacgcagg cgctgatccg cagctggcag acgctcgcgc gcgcgacggg gatactcgac
    17101 ctctcccgcc agatctggct gtgggtcttc gcgccggggt tctacgagga gcagccggac
    17161 acgtttgccg agctcgagcg tcagatcagg gagagcccgt ctcagacggt ggaggagttc
    17221 tgcaaccagg ccgaggcgtg tctcagccat ccgacgctgg agcaggtggc gcagatcgag
    17281 gcgcccacgc tgatcggcgt cggggaaacg gacatcctca cgcctcctgc gcattcgcgt
    17341 gccctgcacg agcggatccc ggggtccgtg ctgcacatct ggcccggcat gggccacgcg
    17401 ccgttctggg agattccgga gcagttcaat cggctcaacc gcgacttcct ggaggctcac
    17461 tgatgatctc gcttgcgatt ctcggcggcg gcttcatggg ctccgcccac gctgcgaact
    17521 tcaaggctct cggcgaccgc gtccgggtga agacggtcgc gtcgcgcacg acggagcgcg
    17581 cagcggcgat cgccgagact gtgggggcgg agatcaccga cgatctcgat gcggcgatcg
    17641 ccgatccggc cgtcgatgtg atcgacatct gcctcccgac gccgctgcac cgcgagtaca
    17701 cggagcgggc gatcgctgcg ggcaagcacg tcttcctcga gaagccgatc gcgctcactc
    17761 tcgaggatgc ggacgcgatc gtctcggctg ctgccggcag tgacagggtc ttcatggtcg
    17821 ggatggtgct tcgcttctgg cccgagtacg tcgagctgca gcgtctggtg gcggcaggcg
    17881 aactgggccg cccgatctcg gtcgtcgcgt cgcggctctc gccgcctgcc gactggaacg
    17941 actggatggc cgaccgatcc cagtccggcg gtacaccggt cgacctgatg atccacgaca
    18001 tcgaccagat gaactggctg ctcgggacgc cccgcaccgt ctacgcgacg gagccgaccc
    18061 ccggtcacgt ccacgccgtt gtcgagtacg acggcgcatc cggtgcggcc gaggggagca
    18121 tgtgcatgcc gcgctcatat ccgttctcgt cgctgatcag ggtgctgtgc gaggacggcg
    18181 tggccgaata cgccttctcg gctgcgccga tcgagggcga gggcaacatc ggcgagtcgt
    18241 cctcggcccg cgggcttcgc ctcttcccgg tcgacggcga cccccgtacg gtcgcagtgg
    18301 agagcgccga tccgtggggc cccgagctgg aggagttcgt ctcctgcgtc gagcagggta
    18361 ggcagcccga gcagggcacg ggggagcagg cgaccctcgc gctacgtgtc gcgctcgcgg
    18421 cgagtcgctc gctcgagagc ggagtgccgg aagtggtgtg atgaacgcgg cggtcgagcg
    18481 agtcggtgct cggatcaacg cgctgcgcgg cgaggcggtc gcgctgctcg aggagctcgt
    18541 tcgggtcaac agcgtgaatc ccgagttcgc gggcgttcag cgcgccgacg tgatcggggg
    18601 cgagacgcgt gtcaacgaga tcctgcgtga gcgctacgag caggcggggc tggagacgca
    18661 ctgggtcgca cccgatcccg agcggaagaa tctcgtcggt gtgcggaagg gaagcggcgg
    18721 cgggcggtcg gtggcgctca acggtcacgt cgacacggtg ccgccggtcg agccggagag
    18781 ctgggtcaac ggcagcccgt ggaatcccga gatccgcgac ggccgcctgt acggcatcgg
    18841 ctcgacggac atgaagggat ccgacgccgc gatgtggctc gtggcgcagg cgctcgagga
    18901 cgagggcgcc gagctcgccg gcgacctcct gctgcactcg gtgatcggcg aggagacgat
    18961 gcagcacgat ctcggaacgt ccgcggtggt cgaggccggt ttcggagcgg acgcggcgat
    19021 cgtgacggag ccgacgtcga tcccgaagcc gctgaccgtc tcaccgatcg cggccggcaa
    19081 ctggtacttc cgcattcacg tcgaggggcg gagtacgcac tgcggaaacc gcgcccttgc
    19141 gatccgcccg ggcgggcccg gtgacgcgat cggcgtcaac gctctcgaga aggcggtcag
    19201 gatcgtgagc tcgctccagg agctggagcg gcagtggggg ctgacgaaga gccatcccta
    19261 cttcatgccc gggttcttct cgctgctgcc gggggtcttc tactccgatc cggggctcga
    19321 catccccttc tacttctcga accgggccga gatcgcgtac tcgctctggt acccgccgga
    19381 cgagacagcc gaggacgcgc gcgccgagat cgaggagtac gtcctgaccg gtagcaggct
    19441 cgacccctgg ctgcgcgcgc acccgccgcg cttcgagtgg atcaacaact ggccagccgc
    19501 ctccactccc tgggaggatc cgatcgtgca gacgatggcg catgcccatg aacgctcggc
    19561 gggcatgacg atcccgcccc cgagcccgca gcacccggtc gcattcggtg cagcgtcgga
    19621 tgcgtcgttc tacgaggctg cggggattcc ggcagtcgtc ttcggccccg gcgacgtccg
    19681 gatcgcgcat tgtcgggacg agagcgtcga cctcgacgag gtcgtcgccg cggcgcactc
    19741 gctcgccgcc tgtgtgattg agtggtgcga tggcaggagc tagaccctgg cgtgcgatct
    19801 gccaggcgct cgctgccgag aacgtggagt acgtcttcgg actgccgggg aaccccgagc
    19861 tgctctacaa cgacctctac gacttcccgc aggtgaagcc ggtgctcgtc cgcatggaga
    19921 cgtcggctgt cttcctggcg atggcgtacg ctcgggtatc cggccgggcc ggggtggtgc
    19981 acgcgagccc ggggccggga atggcgaacc tcgtgccggg cctgctcgag gcccatttcg
    20041 cctgcagccc gctggtgtgc atcgtctcgg gtgccgatcg cgcacacgag ggcgccggcg
    20101 gattccagga cacgccggcg ctggagatgg tccgaccgat caccaagtgg accgttcgca
    20161 tcgacctccc ggagcgcacg acctggacga tgcagcgagc gttcgccgtc gcgcggaacg
    20221 ggaagcccgg gccggtgttc gtagaggtgc cctccgacgt ggcgggcatg acggccgaga
    20281 tccccgacta ccgacgcctg cactccccgc tccgtgcccg cggtgacccc gctgccatcg
    20341 ccgacgcagt ccgcctgctc gaagacgccg agcggccagt gctctgggcc ggcggcggcg
    20401 tagggctctc cgaggccgag gctgagctac tcgccctcgc cgaggccctg gacgccccgg
    20461 tggtcacgac cccgtcgggc cgcggctcga tctcggagga gcatcggctc ggcttcggcg
    20521 tcgtgggcct gtaccggacc gagtcgagcg cgaggccagt cgacgaggcc gacgcgatcg
    20581 tgtacgtcgg cacccgcctg gaggagttcc aatcggggct cggccgctat ctgccgcccg
    20641 ccgcgaaatc gatacagatc gacgtggacc cgttcgagat cgagcgaggg gtgaccgtgg
    20701 acgtgggcat tgccggcgac gcgaagctcg tcctccgcga gctcgcggac gccgtgcggc
    20761 cgatcgaggg tcgggcctgg acgggcgatt tggccgcctt ccgggcggac ttcgagggcc
    20821 gagtcgaggt ggagtgcgct ccgaacggcg gcgccctcaa gacgaagcag atcgtgcacg
    20881 ggctgaacgc cgtcttcgac gacggcttca tcctggtcaa cgagaacggc ggtcaggatc
    20941 tctggtccta ctactgcccg tacctcaagg tgacgaccca ccgtggctgc gtcgcccccg
    21001 ccgagcagac ggtgatgggt ctcggcgtcg ctggttcggt gggtgcgaag ctcgctcggc
    21061 ccgaggcgcc cgtcgtgtgc gtcacaggcg acggggcctt ccagatgtat atgaaggaga
    21121 tcccgaccgc cgtgcagcac ggcgcgccgg tgctctgggt agtgctcgac aacgccggcc
    21181 tgcactgggt gaagtggatc gccgccgcga cgggcgagaa gtacatcgcc gtcgacttcg
    21241 aggcgcaacc cgatctcgtg ggggtcgcgc aggcgagcgg cgctcacgca gagcggatcg
    21301 aggcgcccgg cgagctgctc gacgcactcg cccgcgcgaa ggcggcgatg gagcagggga
    21361 tgccggcggt gctcgtctgc gggatcgaca cgtgggacta cccgcagggc ttcgtcgact
    21421 tccatcgaga gatgtgggga ctggagcttc ccgctcgaga aaggagcacg gcatgagttg
    21481 gatccagacc gggtcgagca tctgcttcaa gccgtacacg ctcgaggagg ccctgagcgg
    21541 cctcgccgcg gcgggattcg aaacgtcgag atcggcaccg tcaaggactt tctgccccat
    21601 ctcgacccgg acaacctttg gcccgcggag atcgccgacg ccgagcgcct gctgaaccag
    21661 cacggccttc gctgtgtctc gatgagcggt cacgcgcctc tccacacgga ggagggtctg
    21721 gcccggctca cgcgtgtgct cgaggccggc acggccctcg gcatcctcgt gctgaacacc
    21781 ttcacgagcg acgccgagac gcccggcacc cgagacggac gtcaagcacg gcctcgggcg
    21841 ctacggccac gtgcatctga aggatcagcg cggcggcaag ggcgcgctcg acttcccgcc
    21901 actcggcgac ggcgagatcg accttcccag catccttcgc gacctggccg acgcgggctt
    21961 cggcgggccg gtcagcatgg agatcgagta cgttatccgc cttggcagga gtgcgtcgag
    22021 aacgccaggc ggagcaaggc gtggtgggac gcgctgcgga tctgagcgct cggggctgct
    22081 ctcgatcagc cgcgggagtc ggcgagcacc gagtagatcc cggccgaggc gcgacggccc
    22141 tcccagcgct cgaacaggtc gccgagcttg cgcggcatct cctcgtcgaa cgtctgctcc
    22201 gcggcctccg cgttcgcggc cgagtcgaag aacgtgatcg ccagcgcctc cccctcccgc
    22261 gagacgaggt gggcatagcc tcggtagccg gcgaggcctt ccatcatcgg gcggatgagc
    22321 gcctcagcct cacccatcgt ctccacagcc ccctcgaagt tgacgccctc gaaccgtgca
    22381 acgcgggcaa ccatggctcc tcctcactgt aggtcgtgct atgcggaggc gacgccccca
    22441 ggacgccgcg tagcttgagt ccccgcggcg ggctggtcaa gaaggccggc cgatcgacta
    22501 gcgcgtcttc acgaagcgct gcaccgcgtc gagcagctcg cccgtcttct cggtgatctc
    22561 cgcatcgtca cccgacgcga gagcgcccgc gacgcagtga ttgacgtgat cctcgagcag
    22621 cttggcaccg acctgctcga gcgcggtctc cacggccgcg atctgggtga ggatgtcgat
    22681 gcagtagcga tcctcctcca ccatccgctc gagtccgcgc acctgcccct cgatccgccg
    22741 gagacgcttg gtcagagcat ccttgtcgtg ggtgtagccg tgcatcaggt tccctcccgg
    22801 aggctgtgga agcgccgcag gcgcaagctg tttgtgacga cgaacacgct cgagaaagcc
    22861 atcgcggcgg ccgcgatcat cggattgagc aggcccgcga cggcgagcgg gatcgccgcg
    22921 acgttatagc cgaaggccca gaagaggttt cccttgatcg tgcgcagggt gcgccgtgag
    22981 agtcggatgg cgtctgccgc cccgcgcagg tcgcccgaca ccagcgtgat gtccgaggct
    23041 tcgatggcga cgtcggtgcc ggtgccgatc gcgagtccca ggtcggactg ggccagggcg
    23101 ggcgcgtcgt tgaccccgtc gcccaccatc gctaccacct cgccgttccg ctggaggcgc
    23161 gttatctcgt tgctcttctc gtgcggcagc acttcggcca ggacgttttc gatcccgacc
    23221 tcggcgccga cggcgcgcgc ggctcggtcg ttgtcgccgg tgagaaggac gggggtcagg
    23281 ccgagtgcct tcagctcctc gattgcctcg ctcgacgtcg gcttgagacg gtcggcgacg
    23341 acgaacacgc ccgccggccg gccgtcgatc gcggctgcga ccaccgtccg gcctgcggct
    23401 tccgcgcgcc cgatctcctc tgcgagctcg gtgggaagct cgaggcccca tgccgcgagc
    23461 atcacgggcc gcccgacgac gaccgcgtga ccctccacga ttgcttccac gccgagcccg
    23521 gcccggctcg tgaacgactc tacgtcagct tgctcgccca gctctcccag cgcttgagcc
    23581 gcgatcgcct gcgcgatcgg atgctcgctc gcggcttccg cggccccgac gagcgcgagc
    23641 agctccgacc gatcgacgcc gggtgcagga gcgaggtccg ccacccacat gcgaccctcg
    23701 gtgatcgtac ccgtcttgtc gagcacgatc gtggagatct ggcgtgtgcg ctcgagcgtc
    23761 tctgggcctc tgatcaggat cccgagctgc gcgccgcggc ccgtcccgac catcagcgcg
    23821 gtcggcgtcg ccagcccgag cgcgcaaggg cacgcgatga tcaacaccgc cacggccgcc
    23881 gtgacaccgg cggaggcgtc acccgtgatg gcgagccacc ccgcaagcgt caccagggag
    23941 atcgcgagca cgaccggaac gaagacggac gagacccggt cgacgagccg ctggatcgga
    24001 gctttgccgg cctgtgcctc ggcgacgagc cgggtgatct gggccagggc ggtgtcggcg
    24061 ccgaccttgg tcgctcgcac caggatgcgg cccgaggtgt tgagcgtcgc gccggccact
    24121 tcgtcgctcg cgacgacctc gaccggcacc ggctcacctg tgagcatcga ctggtcgacg
    24181 gccgagaagc cctcgacgac gacaccgtct gtggcgatct tctcgcccgg ccgcacgacg
    24241 aagacgtcgc ccacctgcag ctcgtcgctg ggcacgagca cctccgcgcc ctcccgcagg
    24301 acgcgagcct ccttcgcgcc gagctcgagc aacgagcgga tcgccgcgcc cgagcggctg
    24361 cgggcacgcg tctcgaggaa gcggccgacg aggatcaggg tcgtgatcac cgcggcgacc
    24421 tcgaagtacg agtgcgagtc gattccggcg aggagcacga ccgtcgacca cgtccacgct
    24481 gccagagttc cgaccgagat cagcgtgtcc atggttgcgg cgccgtgccg ggcgttgagc
    24541 agcgctgcac gatggaactg gaggccgctc cagaagacga ccggtgtcgt cagcgccagt
    24601 gccgcccatt cccaaccgtc gaactgaact tgcggcacca tcgcgagcag gacgacggga
    24661 gccgtcagag cgacggcgac gacgagcttt cgccgtagca ccgccagggc gtcggtgtct
    24721 tcctcggctc cgccctcgag cgccgcgcgg tagccgatgc tctccacgct ctggacgagc
    24781 tcgtcgagcg agacggcgga tgggtcgtac gagacggccg ccgtctctgt cgcatagttg
    24841 acagtcgcct cgacgccctc gatcttgccg agcttgcgct cgatgcgcgt cgcgcacgag
    24901 gcgcacgtca tgccctcgat ggcgagctgg acgcgctccg gtgcttcggg cgtggcggtc
    24961 ttcacgtcgc ctcgtagccg gcctcgtcga ttgccgcccg gattgccgcg tcatcgagcg
    25021 ccgggccgtg cacctcgacc cgcttgctct cgaggtcgac ctcgacctcc gacactccgg
    25081 ggaggccgga gatctcgccg cgcacggcgg cgacgcagtg ctcgcagctc atggcgggaa
    25141 cggagtagat gagcgtctcc atgcctgtga ttatacccta ctggggtata tgtgtaactt
    25201 cagggaagcg cggcctcgac ttcgatttcg accttccagg ccgggtcgag cagcgccgcg
    25261 acgatcgtcg tgttcgcggg cctgatctcg ccgaacactt cgccgtgcgc ccgcgagacg
    25321 ccgtcccagt gggtcacgtc ggtgacgaag acgcgcgtcc gaacgacgtg ctcgaggccg
    25381 gagcccgccc gaacgagcgc ttcttcgatg atctgcaggc agagccgcgc ctgctcgtac
    25441 gagtcctccg gaggcggcgc tccgtcctgc gggatcggtg ccgtcccggc aacgtagacg
    25501 cggtcaccgg cgacgacggc ccgcgagtaa ccgacgatcg gctcataggg cgagtgaccc
    25561 gaaatcagtc gccgcggctt cgagtccggt gccgtcgagc ctgtcacggg acgatcgcct
    25621 ccgcctcgag ctcgaccttc cagcgggcat cggcgaagcc ggagaccacc atgaaggcgt
    25681 tcgcgggcct gacctccgcg aagacctcgc cgtgggccct cgcgacgtcc tggaagtcgt
    25741 cggcgctggt cgcgtagatg cgtgtgcgga cgacgtcctc cgggcgagcg cccgcctcct
    25801 ccagtgcggc caggacaatt tcggggcaac gcttcgtctg accgtacgcg tcggttgggg
    25861 gctcgccgcc gtcctccatg ttcggcccgg tgcccgccac gtggacgtgc ggcccgacgc
    25921 gcacggcgcg cgagaagccc atgatcggct cgtacggcga gccggaggcg acgaggcggc
    25981 gctccaccgg gctagtatcg ccgcccgtgg acgccctgcg ccaggtcgac gactgggacg
    26041 tcggcttcgc ggcggcggga gtcacccgtg tcggtggcgt ggtcgccgcg cacggcgggc
    26101 ggcggcagat ggttcgcctc gcgtccgtgt ccaaggttgt gacggcgctg gcgactctcg
    26161 tcgccgccga ggagggcgtg atcgacctcg acgagccggc gggaccgccg ggcgcgacgg
    26221 tgcggcatct cctggcgcac gcgtctggac tgccgttcga aggacacgtc gtgctcggct
    26281 ctcccgcccg cgggcgcatc tactccaacg agggcttccg cgtgctcggc gagcatctgg
    26341 ccaggcgggc agagatgccg gtagccgagt acatccgggc cgccgtcgtc gtgccgctcg
    26401 ggctcgagct cgatccgacc gggcatcccg gcgcgggcat gcacggccat ctcgacgacg
    26461 tgctggcgct cggcagggaa ttcctcgcgc cgacgcttgt cgcgcccgag accttcgccg
    26521 aggcgaccca ggtgcagttt ccaggcctcg ccggcgtcct gccgggcttc ggccggcagg
    26581 cgacgaacga ctggggcctc ggcttcgact tgaaggacgc gaagtcgccg cactggacgg
    26641 gcgagcggaa ctcgccgctg acgttcggcc acttcggggg cagcgggacg ttcctctggg
    26701 tcgacccgtc tgtgggcgtc tcttgcgccg cgctgaccga acgccccttc ggtgactggg
    26761 cgaaggcggc gtggcccgcg ctctcggacg cagtcttggc cgagctcgcc tagacctcga
    26821 gaaagtccag cagcagacga ttcacgaggt cgggccgctc gaggctcggc aggtgggcgg
    26881 ctcccgggat gcgccggagc gtcgcgtttt gggcttctgc tgcgatccgc gccgcgatcg
    26941 actgcatgtc gagcgtgtcc atctcgccga tcagcacgag cgtcggtgtc gccaggcggt
    27001 cgagacgctc gctcgcgccc gacgccggcc acgtgtcctc ggcggcgtca ccgaccggca
    27061 gctgcagctc gaacgagcgc agcaccatct cccgcacgag cgaccggacc ccggggtcga
    27121 cgtcctgccc acggtcgagc cagaggcgta ggttcgagtc ggccgcggcc ccgaggtcgc
    27181 cgcgctcgag agcggcctcc tcttcctccc agccggacgt tacctcggcc gaccactccc
    27241 acccgtcgag cccgggggcg atgaggacga gccgctccac caaggaagga gcggtcagag
    27301 caagctcgag cgcgacgcgg ccgccgaaag agctccccac gactgcggcg gggcagccgc
    27361 cgagcgccgc ccgcacgtcg tccacgtgcg agaaccgccc cgcctcgagc gcacgcgtgc
    27421 cgaaaccccg aaggtcgggc gcgacggcac gaaatcccgc tgcctcgaga gccgtgatct
    27481 gagggcgcca catccgcgcg tcggctatgc ccgcgtggac gagcaggacg ctcctcaagc
    27541 ggcgtcgccc ccacggtcgc tgaacagggg gaggatgagc tcctcgaacg tctcgcggtc
    27601 gcagctcgcg atcacacacg gcgtcatcgc ggtcaccgtt gcggtcctgg cgacgccgag
    27661 ggccggagcg acctcgccga agtactcacc cggcctgatc atgccccgtg cgccacggtc
    27721 ggtctggctc accgccgcaa caccggaaag gagtacgaag aagcggtctc cgggctcgcc
    27781 ctcgctgatg atcgcggtgc cgggcgcaac gtcctcgcgg cgcatccgcc cggcgaggcg
    27841 cgtgagctgc tccccgggca gctcggcgag gaggccgacc cgcgagagct cggttatcga
    27901 agccggcacg tgctgggggc tcacagcggg gtacggtagc ggcgatggcg atcctccacg
    27961 gc
//
LOCUS       DBNGNK010000405         3530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_500931,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000405 DBNGNK010000000
VERSION     DBNGNK010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_500931"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..2891)
                     /locus_tag="AC150V_12150"
     CDS             complement(114..2891)
                     /locus_tag="AC150V_12150"
                     /inference="COORDINATES: protein motif:HMM:NF053442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR3763713.1"
                     /translation="MTLSRLSILAPGALVLTLIAPARSAAEPTRLLRQPALSAEHVAF
                     VYAGDVWIVGRDGGDARRLTATPAVESDTHFSPDGRSIAFTSNRHGNPEVHVVPIEGG
                     HPKRLTWYPATATVRGWTSDGSRVLYASSRATAPSAFYRLWTVPAGGGASEIVPTPWG
                     ADGSYSPEGDRMIVDRVSRSETEWRHYRGGQNSSLTLLDLTDLAETHLPNERTIDVQP
                     LWLGDTIYFLTDRDWTMNVWTYDPASAALAQLTHFTDVDVKWLNGHDGTLVLEREGWI
                     HLFDVESGELERLDITVRGDFPWARPAWVEVGDAVRTAAISPTGKRAVFEARGEIFTV
                     PAEKGDARNLSRSSGAADRAPVWSPDGGRIAWVSDDGDGYELLIADQGGRSEPRRLSI
                     GESKMVWETTWSPDGEHIAFVDDDVRVRVLDVASGELTTADVGGINIERGRMGLVWSP
                     DSRWLAYAKTFPNSFRRVVVWSLASGEATPLSDPMADAVSPSWDRDGRYLYFLASTDL
                     ALLSGWANTSVRQAPDPTYAVYGAVLRAEDPTPFEPESDEEPVEENEGEVEDEEEDGK
                     DRKRNKGKKKTESGDGEEAEDEGDAVEVRIDLDGIERRTLALPMPVSTYRATWAGPAG
                     SVFIGEAPASDGSGLTLHKFTLEEREAEVFLENASRVSISNDGRKMLYGSAPGRWAIV
                     GTDSPPEAGGGALDLSLRMRLDRRTEWRQIFDEAWHYQRDFFYDPDHHGRDWDAVYER
                     YSPLVAWVRHRADLTYLLDQMNGELSVGHSFVFGGDYPEVEESAIGLLGADLVATPSS
                     DGGRWRIERIYTFESWNPEVDAPLDRPGLEVAEGDYLLAIDGIELTARDEPYRLLDGT
                     AGRQTILHVNDTPSMEGARAVTVEPVDSESELRRRAWVEDNRRRVDELSGGKLAYVWV
                     PNTSTRGVTSV"
     gene            2967..3449
                     /locus_tag="AC150V_12155"
     CDS             2967..3449
                     /locus_tag="AC150V_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763714.1"
                     /translation="MSASSPDDFDFLLGSWRVRSRRLVTRLAGSDEWVEFPARLEGGG
                     KILGGLALIDQFKTELDGEAFEGLSLRVFDPATGRWTIYWADTARPRLTEQVVGGFEN
                     GVGTFHGEEVFEGRTVELRCVWSRITPASARWEQAYFDEKRDGWETNWVMDFERTEDA
                     "
BASE COUNT          520 a         1129 c         1311 g          570 t
ORIGIN      
        1 cggagaaagt ttttgttccg acatttttcg aacttccgga gaaaatgttt tccagccaat
       61 gtttcacaaa atgagaagaa gaaaaacaaa aataagtcct cttgagaatt tgtttaaact
      121 gaggtgacgc cccgggtcga ggtgttgggg acccagacgt aggccagctt tccgcccgag
      181 agctcatcga cgcgccgccg gttgtcctcg acccaggcgc gacggcgcag ctcgctctcg
      241 ctgtcgaccg gctcgacggt gacggcgcgg gcgccctcca tggagggcgt gtcgttgacg
      301 tgcaggatgg tctgtcggcc ggcggtgcca tcgaggaggc gatagggctc gtcccgggcg
      361 gtgagctcga tgccgtcgat ggcgaggaga tagtcgccct cggcgacctc gaggccgggc
      421 cggtcgagcg gtgcgtcgac ctctgggttc cagctctcga aggtgtagat gcgctcgatc
      481 ctccagcgcc cgccgtcgga ggacggggtg gcgacgaggt cggcgccgag caggccgatc
      541 gcgctctcct cgacctccgg atagtcgccg ccgaagacga aactgtgccc gaccgagagc
      601 tcgccgttca tctggtcgag cagataggtg aggtcggcgc ggtggcgcac ccaggcgacc
      661 agcggcgagt accgctcgta gacggcgtcc cagtcccgcc cgtggtggtc ggggtcgtag
      721 aagaagtcgc gctggtagtg ccaggcctcg tcgaagatct ggcgccactc cgttcggcgg
      781 tcgagacgca tgcgcagcga gagatcgagg gcgccgccac cggcctccgg agggctgtcg
      841 gtcccgacga tcgcccaccg cccgggcgcc gacccataga gcatcttccg gccgtcgttc
      901 gagatcgaca cgcgcgaggc gttctcgagg aagacctccg cctcgcgctc ctcgagggtg
      961 aacttgtgga gcgtcaggcc ggagccgtcg ctcgctgggg cctcgccgat gaagacgctg
     1021 cccgcggggc ctgcccaggt ggcgcggtag gtggagaccg gcatcggcag ggcgagcgtg
     1081 cggcgctcga taccgtcgag gtcgatgcgg acctcgaccg cgtcgccttc gtcctcggct
     1141 tcttccccgt cgccggactc ggtcttcttc tttcccttgt tgcgcttccg gtccttgccg
     1201 tcctcttcct cgtcctcaac ctcgccttcg ttctcctcca cgggctcctc gtcgctctcg
     1261 ggctcgaacg gggtcggatc ctcggcccgc aacacggcgc catagacggc gtaggtcggg
     1321 tccggcgcct ggcggacgct ggtgttggcc cagccggaga gcagcgccag gtcggtggag
     1381 gccaggaagt agaggtagcg gccgtctcgg tcccaggagg gggaaacggc gtcggccatg
     1441 gggtcggaca gtggcgtggc ctcgcccgag gcgagcgacc agacgacgac gcggcggaag
     1501 ctgttgggga aggtcttggc gtaggcgagc cagcgggagt cgggcgacca gacgagcccc
     1561 atgcggccac gctcgatgtt gatgccgccg acgtcggcgg tcgtgagctc gccgcttgcg
     1621 acgtcgagca cccggacgcg cacgtcgtcg tcgacgaaag cgatgtgctc gccgtccggc
     1681 gaccaggtgg tctcccagac catcttggac tcgccgatcg acaggcgccg cggctcggag
     1741 cggccgccct ggtcggcgat caggagctcg tagccgtcgc cgtcgtcgga gacccaggcg
     1801 atccgtccgc catcgggcga ccagaccggg gcgcgatcgg cggcgccgga gctgcggctg
     1861 aggttgcgcg cgtcgccctt ctcggccggc acggtgaaga tctcgccgcg cgcctcgaag
     1921 acggcgcgct tgccggtggg cgagatggcc gccgtgcgga ctgcgtcgcc gacctccacc
     1981 caggcggggc gcgcccaggg gaagtcgccg cgcacggtga tgtcgaggcg ctcgagctcg
     2041 cccgactcga cgtcgaacag gtggatccag ccctcgcgct cgagcaccag ggtaccgtcg
     2101 tggccgttca gccacttgac gtcgacgtcg gtgaagtgcg tgagctgggc gagcgccgcc
     2161 gacgcgggat cgtaggtcca gacgttcatc gtccagtcgc ggtcggtgag gaagtagatg
     2221 gtgtcgccca gccacagcgg ctgcacgtcg atggtgcgct cgttgggcag gtgggtctcg
     2281 gcgaggtcgg tcaggtcgag cagggtcaga gaggagttct gtccgccgcg gtagtggcgc
     2341 cactcggtct ccgagcggga gacgcggtcg acgatcatgc ggtccccctc gggggagtac
     2401 gagccgtcgg ctccccaggg cgtcgggacg atctccgatg ccccgccgcc tgccggcacg
     2461 gtccacagtc ggtagaaggc gctcggcgcg gtggctcggc tcgaggcgta gaggacgcgg
     2521 gagccgtcgc tcgtccagcc gcgaaccgtc gccgtcgccg ggtaccaggt gagccgtttc
     2581 ggatggccgc cctcgatcgg cacgacgtgg acctccgggt tgccatggcg attcgacgtg
     2641 aaggcgatcg agcggccgtc gggcgagaaa tgcgtgtcgc tctcgacggc gggcgtcgcg
     2701 gtcaggcgcc gagcgtcgcc gccgtcccgg ccgacgatcc agacgtcacc ggcgtagacg
     2761 aaggcgacgt gctcggcgct caacgcgggc tggcgcagga gacgggtggg ctcggccgcc
     2821 gatcgcgccg gggcgatcaa ggtcagaacg agcgcgccag gcgcgagaat gctcagccga
     2881 ctgagagtca tggcgggtct ccgtggcgga tccaggggat gtctcggcat tctaccggct
     2941 cgggctcgag cggtagactg acgccggtgt cagcctcgag ccctgacgat ttcgattttc
     3001 tcctcggcag ctggcgggtg cggagccgcc ggctggtgac ccgtttggcg ggctccgacg
     3061 agtgggtcga gttccccgcc aggctcgagg gcggcgggaa gatcctcggc gggctggcgc
     3121 tgatcgatca gttcaagacc gagctcgacg gcgaggcctt cgaggggctg agcttgcgcg
     3181 tcttcgatcc ggccacgggt cggtggacga tctactgggc ggacaccgcg cgtccccggc
     3241 tgacggagca ggtcgtcgga gggttcgaga acggcgtggg gaccttccac ggtgaggagg
     3301 tcttcgaggg caggacggtc gagctccggt gtgtctggtc ccgtatcacg ccggcgagcg
     3361 ctcgctggga gcaggcctac ttcgacgaga agcgcgacgg ctgggagacc aactgggtca
     3421 tggacttcga gcgcaccgag gacgcctagc tcctcagctc aggtcgacgg cgacgatcca
     3481 gcgctggtcg cgcgcgtaga gggtgctgcc gacgacggtc gggaccgtcc
//
LOCUS       DBNGNK010000406         9406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_788061,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000406 DBNGNK010000000
VERSION     DBNGNK010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 9406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9406
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_788061"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..402
                     /locus_tag="AC150V_12160"
     CDS             94..402
                     /locus_tag="AC150V_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR3763715.1"
                     /translation="MATILLVGVDLLFRSKLEGLLPGHHFVTSDSAHPPDLVICDIAR
                     VDPDDVVESWPDAPIVGYTNHTDKAGLQRAHTAGFDQVIVKSALVERAPHVVSELTEA
                     "
     gene            451..756
                     /locus_tag="AC150V_12165"
     CDS             451..756
                     /locus_tag="AC150V_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR3763716.1"
                     /translation="MTYIIAEPCIDIKDRSCVDVCPVDCIHEFARVLVIDPVECIDCG
                     ACEPECPVEAIFPEDALPDKWEPFVEINYAFPDSTEKIDPLVDKYATEHNVQNEPIE"
     gene            complement(763..1419)
                     /locus_tag="AC150V_12170"
     CDS             complement(763..1419)
                     /locus_tag="AC150V_12170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR3763717.1"
                     /translation="MTGPNAERAGAAGERLTVERDERRRLAELIHDGPVQHLAAIAQM
                     LDAAKHAIAAEEADSALSIVNRALELTRDASGDLREIVAGLEPVALSEHGFGTAVRLL
                     ADRIRARRDIQILVDVDPADQLGEGARTALYQIVRESLDQAVRRGPPSRITVTVSRPP
                     NGGAELVVTDDGSAERRRAVLDGLEERATELNAEIDVERAPDGGTVVRVTLPPSAAEL
                     "
     gene            complement(1416..2039)
                     /locus_tag="AC150V_12175"
     CDS             complement(1416..2039)
                     /locus_tag="AC150V_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR3763718.1"
                     /translation="MPDEITCLIADDHEVVREGLRLSLLRSPHIRIVGEAPDGETAIT
                     LTERRRPNVVIMDLRMPGMDGIEATEEIAKRIPESNVLIFTAYSERALLARGLESGAK
                     GYILKEAPHEMLLRAIEKVAGGDTFIDPALMPSFVQGKDGKEILTAREREILQLLADG
                     MSNADVAEHLYISQETVKSHVRHILSKLEADTRTQAVAIALREAMID"
     gene            complement(2061..2957)
                     /locus_tag="AC150V_12180"
     CDS             complement(2061..2957)
                     /locus_tag="AC150V_12180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR3763719.1"
                     /translation="MRTSYREEPCRTALNRVKGMPFAWSLNPYTGCAHRCTFCYVRAF
                     ERRADRAYGDGYGRSIRVKVNVAEVLGRELARRSWRREEVAIGAATDPYQPAEGRYRL
                     TRACIEQLARAETPFSIITRGPLVVRDIDALQEASERAGVGVCLSIPTHDERVWRTTE
                     PGTAPPASRLRAVTRLAEAGLDVSVAIAPILPGLSDGHDQLARTVRAARAAGARRVWS
                     KALDLRPGAREHFLAALAHDWPDRAALYERLYANRSYLASRETQRLDVAVRRLAGELE
                     LQFAPRRQPVRPPRPTQLTLAV"
     gene            3013..3948
                     /locus_tag="AC150V_12185"
     CDS             3013..3948
                     /locus_tag="AC150V_12185"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR3763720.1"
                     /translation="MEVLSDGIRRVTMPLPTRPGHVHCYLLPGNDGWTLVDTGLGLPD
                     AKERWAEVLGTLDAPVAQIFVTHFHPDHVGAAADLHQLTGAPVIQGALDYAQCELVWG
                     NPNWAHRLADWFHLQGAPSSVTRELVEQGSAYLPFVRYQPDPVLVEPGESVDGWDLIA
                     APGHADGQLCLLRDGVLIAADHVLHSISPTVGLWPASRADPLGDYLLALDHTIQLAPR
                     VALTGHRDTVADPTGRAAELIDHHRERLAAAQSALEGEPRSGYELSFRLFGADLAPAG
                     RRFAVAETLSHLERLVVEGRAQRAETDEIVTYTAA"
     gene            4043..5359
                     /locus_tag="AC150V_12190"
     CDS             4043..5359
                     /locus_tag="AC150V_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR3763721.1"
                     /translation="MSDAVRIIVIFLLILGNALFVAAEYALVTARRSRLEERAESGSR
                     SARTALKLMDEPVRFISTVQVGITVFGIALGAIGEPLLSSYFESLPRGVAFAISFTVL
                     TYLSVTLGELVPKAIALQRAERLAVALAVPLQALSWLAYPIVWVLQISANVVLRLFRM
                     KPAPAGVMTFTREDILHSVAAAEDVGEIETSEEEMLYKVFDFAGKEVSEVMVPRPDVA
                     AISIDLPPDEALAVVFESPYTRYPVIRGSLDEIVGIIHVRQLFQALHDLGIEAVELED
                     LSRAPFVVPETKDLAALLADFRREKQHLAIVVDEYGSTAGIVTLEDVLEEIVGDIEDE
                     FDLPDESIERIAENTIRIAGNFPIDDFNDEFETALEHSDLHTVAGYVLDMLGRAAEPG
                     DEVLAAGMRLQVVEVDGSRIQRLEIELGVEPPEPGETEEPEKPAAA"
     gene            complement(5412..6632)
                     /locus_tag="AC150V_12195"
     CDS             complement(5412..6632)
                     /locus_tag="AC150V_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR3763722.1"
                     /translation="MLREYTSDCVHCGFCLPTCPTYSLWSEEMDSPRGRIQLMEARLD
                     GTVGLSPTVVQHFDRCLGCMACLTSCPSGVRYDRLIESTREQVEQEYERSRSDRLMRD
                     ALFRVLPYPKRMRAALRLAPLGRAAPVPRKLRPLVELTPRWRSSEAPAAVTPPAGRAR
                     GRVGILTGCVQSVVFGDVNAATARVLAAEGYEVVAPPQGCCGALSVHAGRVEEGRSLA
                     RKLVDAFAGIDTVVVNSSGCGSHLKELGWLLDNEQAEKFSTRVRDLGELLGGVEPRAT
                     RYPLTLRAAYQDSCHLRHAQRLPLAYGRSLAQIPGLEVVEPADQDLCCGSAGIYNLVQ
                     PDAARELGARKAAAVLATSPQVLASANPGCLVQISQSLHSAGEPLPSVHPIELVDASI
                     RGVPAGRLRITARR"
     gene            complement(6641..7537)
                     /locus_tag="AC150V_12200"
     CDS             complement(6641..7537)
                     /locus_tag="AC150V_12200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR3763723.1"
                     /translation="MLRPASIAAASSDLAAAHSAGRRVRIGDDLDTSALNRVLEHEPG
                     DFTCTVEAGITLSALDAVLAKHRQRLALDPPGDPTVGTCLAASLSGPLRHRFGTPRDL
                     TLGVTLVLADGTVANAGGKVVKNVAGYDLGKLVCGSHGTLALIARACLRLHPVPEATA
                     SLIVETDDVAGAVVTILDSQLEPSALDVLHPGRVAALFEGGRAGVAAQLARAKSLVGG
                     EETSADVWAESRERQHAARGILRFSPGELATVLTGLEEAILRPASGVAYLPYEVDSDL
                     SPGARMLNRAVKEHFDPEGILV"
     gene            complement(7539..8993)
                     /locus_tag="AC150V_12205"
     CDS             complement(7539..8993)
                     /locus_tag="AC150V_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR3763724.1"
                     /translation="MGLSAAAVEELRRELGPGNVITEPEQLRTYDCDGLTGWRAQPEL
                     VALPDSVEAVQRIVRACHRERVPWVVRGAGTGLSGGALPVADGIVISVARMNRILDVD
                     VPARRVVVEPGVTNLDVTRAVAGDGFFYAPDPSSQQVCTIGGNVAENSGGAHCLKNGF
                     TVHHVTGLELVLPDGEVAELGGKALDTEGYDLLGAFVGSEGTLGVATKITLRIVRSPE
                     RVTTLLAAFRAPEAAGTAVSHVVGAGILPAAIEMMDRLTIEAAEAAFHPGYPDGAEAV
                     LLVELDGVDVQVDEDAAEVERICREDGAFEVRIAQDESERALLWKGRKGAFPAMGRVS
                     PDYYVQDGVVPRTRLPEVLRRIGELSEEHGLRVGNVFHAGDGNLHPLVLYDASAGEAE
                     RAKELAEEILAACLEAGGSLTGEHGVGVDKACSMPKMFSENDLATFERLRRAFDPYGL
                     ANPGKVVPTPRLCGEIPGPYRMHPLERTGVAERF"
     gene            9086..>9406
                     /locus_tag="AC150V_12210"
     CDS             9086..>9406
                     /locus_tag="AC150V_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007274258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR3763725.1"
                     /translation="MVPIDRPGKIICVGLNYRDHAEEQGVELPEAPLFFAKYTTSLIG
                     PGDPIVIPPIVEQPDYEAELGVVIGQTVKGISRENAMEAVAGYVVANDVSARDLQFAD
                     GQWTR"
BASE COUNT         1454 a         3255 c         3231 g         1466 t
ORIGIN      
        1 tccggatcta cctcaaccgc ttcctggaat gggtaggtat cggctcgctc tagacgcctt
       61 ccgaccgtcg gctcgacgtg gtacgttgcc gtcgtggcga cgatcctgct ggtcggggtc
      121 gatttgctct tccgaagcaa gttggaaggg ctgctgccgg gccatcattt cgttaccagc
      181 gacagcgcgc acccgcccga tctcgtcatc tgcgacatcg cccgggtcga ccccgacgac
      241 gtcgtcgagt cctggccaga cgcccctatc gtcggctaca cgaaccacac ggacaaggcg
      301 ggcctgcagc gggctcacac cgccggattc gaccaggtga tcgtgaaatc ggcgctcgtc
      361 gagcgcgccc cccacgtggt ctccgagctc acagaagcct gatcgcgata gcagcgcagc
      421 ggcggcgagc gcgtgggtac actcgccccc gtgacgtaca tcatcgccga gccctgcatc
      481 gacatcaaag atcgctcctg cgtcgacgtg tgcccggtcg actgcatcca cgagttcgcg
      541 cgcgttctcg tcatcgaccc ggtggagtgc atcgactgcg gcgcgtgcga gccggagtgt
      601 ccggtggagg cgatcttccc ggaggacgcg cttcccgaca agtgggagcc gttcgtggag
      661 atcaactacg ccttccctga ctcgaccgag aagatcgacc cgctcgtgga caagtacgcc
      721 acggagcaca acgtccagaa cgagccgatc gagtagcggc ccctagagct cggccgcaga
      781 cgggggtagc gtcacgcgga cgaccgttcc cccatcgggc gcgcgctcga cgtcgatctc
      841 cgcgttgagc tcggtggcgc gctcctcgag cccgtcgagg acggcacggc ggcgctcggc
      901 agagccgtcg tccgtgacga ccagctctgc cccaccgttg ggcgggcggg agacggtgac
      961 cgtgatccgc gacggtggcc cgcggcggac ggcctggtcg agcgactcgc ggacgatctg
     1021 gtagagcgcc gtccgcgccc cctcaccgag ttggtcggca gggtcgacgt cgacgaggat
     1081 ctggatgtcc ctgcgcgccc gaatgcgatc ggccagcagc cgcacggcgg tgccgaatcc
     1141 atgctcgctc aaggcgaccg gctcgagtcc cgcgacgatc tcgcgcaggt cgccgctggc
     1201 gtcgcgcgtc agctcgaggg ctcgattgac gatcgacaga gcgctgtcgg cctcctctgc
     1261 ggcgatcgcg tgcttggcgg cgtcgagcat ctgcgcgatc gccgcgagat gctgaacggg
     1321 gccgtcgtgg atcagctcgg ccagcctgcg ccgctcgtct cgctcgacag tgaggcgctc
     1381 gccggcggcg cctgcccgct cggcgttcgg cccggtcaat cgatcattgc ctcgcggagc
     1441 gcgatcgcga cagcctgggt tcgggtgtct gcctcgagct tcgagaggat gtggcggaca
     1501 tggctcttca ccgtctcttg gctgatgtag aggtgctcgg cgacgtcggc gttcgacatc
     1561 ccgtcggcga ggagttggag gatctcgcgc tcgcgcgccg tcaggatctc cttgccgtcc
     1621 ttgccctgca cgaaggacgg catcagggcc gggtcgatga aggtgtcacc cccggcgact
     1681 ttctcgatcg cgcggagcag catctcgtgc ggggcctcct tcaggatgta gcccttggct
     1741 ccggactcca gcccgcgtgc gagcagtgcc cgctcgctgt acgccgtgaa gatcaggaca
     1801 ttcgactccg ggatccgctt cgcgatctcc tccgtcgcct cgatgccgtc cattccgggc
     1861 atccgaagat ccatgatcac gacgttgggc cgcctacgct cggtcagcgt gatcgcggtc
     1921 tcgccgtcgg gcgcctcacc gacgatccgg atgtgcggtg agcggaggag cgatagccgc
     1981 agtccttccc gcacgacctc atggtcgtcg gcgatgagac aggtgatctc atccggcacg
     2041 ggtcgattgt acgggcggac ctagacggcg agggtcaact gcgtgggccg ggggggccgg
     2101 acgggctgtc tccggggggc gaactggagc tccagctcgc ctgccagtcg ccgtacggcg
     2161 acgtcgagac gctgcgtctc gcgagacgcc aagtacgagc ggtttgcgta gagccgctcg
     2221 tacagggcgg ccctgtcggg ccagtcgtgg gcgagagcgg cgaggaagtg ctcgcgggcg
     2281 ccgggtcgga ggtcgagcgc cttcgaccac acccgccgcg cgcccgcggc gcgggcggct
     2341 cgcaccgtgc gcgcgagctg gtcgtggccg tcggagagcc ccggcaggat cggcgcgatg
     2401 gcgaccgaca cgtcgagccc ggcctccgcc agccgtgtga cggcccgaag ccgcgacgcc
     2461 ggcggggccg tgcccggctc ggtcgtgcgc cagacgcgct cgtcgtgcgt cgggatcgag
     2521 aggcagactc cgaccccggc gcgctccgag gcctcctgaa gcgcgtcgat gtcgcgtacg
     2581 acgagcggcc cccgagtgat gatcgagaag ggcgtctccg cccgtgcgag ctgctcgata
     2641 cacgcacgcg tgagccggta tcggccctcc gcaggctggt acgggtcggt ggcggcgccg
     2701 atggcgacct cctcccgccg ccacgagcgc cgggccagct cgcgcccgag tacctcggcg
     2761 acgttcacct tgacgcgaat cgaccggccg tagccgtcgc cgtacgcccg gtcggcgcgt
     2821 cgctcgaagg ccctgacgta gcagaacgtg cacctgtgtg cgcagcctgt gtaggggttg
     2881 agcgaccagg cgaaaggcat ccccttgacg cggttgaggg ccgtgcggca gggctcttct
     2941 cgatacgagg tgcgcacgcc gcgagtatac gaacgtgtgt tcccttcgtc cactctcgtc
     3001 tacattcgtc ccgtggaagt gctcagcgac ggcatcaggc gcgtgacgat gccgctgccg
     3061 acccgtcctg gacacgtcca ctgctacctg cttccgggaa acgacggctg gacgctcgtc
     3121 gacaccgggc tcggcctccc cgacgcgaag gagcgctggg cagaggtgct gggaaccctt
     3181 gacgcgccgg tcgcgcaaat cttcgtgacg cacttccacc ccgatcacgt cggcgccgcc
     3241 gcggatctcc accagctcac cggtgcgccg gtgattcagg gggcgctgga ttacgcgcag
     3301 tgcgagctcg tctggggaaa cccgaactgg gctcaccggc tcgcagactg gtttcatctc
     3361 cagggggcgc cgagctcggt cacccgggag ctggtcgagc agggatcggc ctaccttcca
     3421 ttcgtccgct atcagcccga cccggtgctg gtcgaaccgg gagagagcgt cgacggctgg
     3481 gatctgatcg ctgcccccgg ccacgcggac gggcagctct gcttgctccg ggacggcgtt
     3541 ctcatcgccg ccgaccacgt cctccactcg atctcgccca ccgtgggcct ttggccggcg
     3601 agccgagcag acccgctcgg cgactacctc cttgcgctcg accacacgat ccagctcgcg
     3661 ccgcgggtcg cgttgacggg ccaccgcgac accgtcgccg atccgaccgg ccgggctgcc
     3721 gagctgatcg atcaccaccg cgagcgactc gctgcggcac agtcggcgct ggagggtgag
     3781 ccccgctccg gctacgagct ctccttccgg ctcttcggcg ccgatctggc gccggccggg
     3841 cggcgcttcg ctgtggcaga gacgctgtcg catctcgagc gcctcgtggt cgagggacgc
     3901 gcacagcggg cggagaccga cgagatcgtc acctatactg cggcctgagt ggacggcgag
     3961 ctaccgccca gtacccgcgc cccgtacggc ggggcgactc ctctgacaaa cggcctcccc
     4021 gccagggggg cgtgagcatg ccgtgagcga cgccgtacgc atcatcgtca tcttcctgct
     4081 gatcctgggc aacgcgctct tcgtcgcggc cgagtacgcg ctcgtcaccg cccgacgcag
     4141 ccggctcgag gagcgcgccg aatcgggcag ccgctcggcg cgcactgctc tcaagctgat
     4201 ggacgagccg gtgcgcttca tcagcaccgt gcaggtcggg atcacggtct tcgggatcgc
     4261 cctcggtgcg atcggcgagc ccctgctctc ctcgtacttc gagtcgctgc cgcgcggcgt
     4321 cgcctttgcc atctcgttca cggtgctgac ctacttgagc gtcacgctcg gcgagctcgt
     4381 tccgaaagcg attgcgcttc agcgcgccga gcggctcgcg gtcgcgctgg cagtaccgct
     4441 gcaggcgctc tcgtggctgg cgtatccgat cgtgtgggtg ctgcagatct ccgcgaacgt
     4501 cgtgttgcgg ctgttccgca tgaagccggc tccggcgggc gtgatgacgt tcacgcgaga
     4561 ggacatcttg cactccgtcg cggccgccga ggacgtcggc gagatcgaga cctcggagga
     4621 ggaaatgctc tacaaggtct tcgacttcgc cggcaaggag gtgagcgagg tgatggtgcc
     4681 gcgtcctgac gtggccgcga tctcgatcga cttgccgccg gacgaggcgc tggccgtcgt
     4741 gttcgagagc ccgtatacgc ggtacccggt gatccgcggc tcgctcgacg agatcgtcgg
     4801 aatcatccac gtacgccagc tgttccaggc cctccacgac ctgggcatcg aagccgtcga
     4861 gctcgaggat ctttcgcgcg cgcccttcgt cgtgcccgaa acgaaagacc tcgccgctct
     4921 gctcgcggac ttccgccgcg agaagcaaca tcttgcgatc gtcgtcgacg agtacgggtc
     4981 gactgccggc atcgtcacgc tcgaggacgt cctcgaagag atcgtgggtg acatcgagga
     5041 cgagttcgac ctgccggacg agtcgatcga gcggatcgcc gagaacacga tccgtatcgc
     5101 cgggaacttc ccgatcgacg acttcaacga cgagttcgag acggcgctcg agcattcgga
     5161 cctccacacc gtcgccgggt acgtcctcga catgctcggg cgggcggccg agccgggcga
     5221 cgaggtgctt gcggccggga tgcgcctcca ggtggtcgag gtcgacggat cgcggatcca
     5281 gcgcctcgag atcgagctcg gggtcgaacc gccggagccc ggcgaaaccg aggagcccga
     5341 gaagccggcc gcggcctagg cgtcgcggcc gcgaaccgac cgccgcgtcg cagccatgag
     5401 cgcccgagcg actagcgccg tgccgtaata cgcagcctcc cggccggaac gccccgaatc
     5461 gaagcgtcca ccagctcgat cgggtgaact gaaggcagcg gctcgccggc gctgtgaagc
     5521 gactgcgaga tctgcacgag acagccggga ttcgcgctcg cgagcacctg aggactcgtg
     5581 gcgaggacgg ccgccgcttt gcgcgcgccg agctcgcgcg ccgcgtccgg ctgcacgagg
     5641 ttgtagatcc cggccgatcc gcagcagagg tcctggtctg cgggctccac gacctccagg
     5701 cccgggatct gggcaagcga tctgccgtag gcgagcggga gacgctgagc gtggcgcagg
     5761 tggcaggagt cctggtaggc ggcgcggagc gtaagcgggt agcgggtcgc acggggctcg
     5821 acaccgccga gcagctcgcc gagatcgcgc acgcgcgtcg agaacttctc ggcctgctcg
     5881 ttgtcgagca gccagccgag ctccttgaga tgagagccgc agccggagga gttcaccacc
     5941 acggtatcga tcccggcgaa ggcatcgacg agcttgcggg ccagcgacct gccctcttcg
     6001 acccggccgg cgtggacgga cagcgcgccg cagcacccct gcggaggcgc gacgacctcg
     6061 tacccctcgg cggcgaggac gcgcgccgtt gccgcgttga cgtcgccgaa gacgactgac
     6121 tggacgcacc ccgtgaggat gccgacgcgt ccccgtgccc gaccggccgg cggagtcacc
     6181 gccgccggag cctcgctgga gcgccagcgc ggcgtgagct cgaccagagg cctcagcttg
     6241 cgcgggacag gcgccgcgcg gccgagcggc gcgagcctga gagcagcccg catacgcttc
     6301 gggtagggaa gcacccggaa gagcgcgtca cgcatgagcc ggtcgctgcg agaacgctcg
     6361 tattcctgct cgacctgctc gcgggtggac tcgatgagcc ggtcgtagcg aacccctgac
     6421 gggcacgacg tgaggcacgc catgcaaccc agacagcggt cgaagtgctg gacgacagtg
     6481 gggctgagcc cgaccgtgcc gtccagacga gcctccatga gctggatgcg cccgcgcggc
     6541 gagtccattt cctcactcca gagcgagtag gtcgggcagg tcggcaggca gaagccacag
     6601 tggacgcagt cgctcgtgta ctcgcgaagc attccgtgcc tcagacgagg atgccctcgg
     6661 gatcgaaatg ttccttcacg gcccggttga gcatccgcgc cccgggtgag aggtcgctgt
     6721 cgacctcgta ggggaggtag gcgactcccg acgccggtcg gaggatcgcc tcctcgaggc
     6781 cggtcagcac cgtcgcgagc tcgcccggcg agaaacgcag gatgccgcgc gccgcgtgct
     6841 gccgctcgcg cgactcggcc cagacgtccg cgctcgtctc ctctccgccg accagcgact
     6901 tcgcgcgtgc cagctgcgcc gcgactcccg ctcgtcctcc ctcgaataga gcggcaacgc
     6961 ggcccggatg caagacgtcg agcgcgctcg gctccagctg gctgtcgagt atcgtcacga
     7021 cggctcccgc gacgtcgtcc gtctcgacga tcaagctggc cgttgcctcc ggaacaggat
     7081 gcagccgcag gcaggcgcgc gcgatcagcg ccagcgtgcc gtgtgacccg cagacgagct
     7141 tgccgaggtc gtatccggcg acgttcttga ccaccttgcc gcccgcgttc gccactgtcc
     7201 cgtccgccag gacgagagtc acacccaaag tcaggtcacg tggcgtgccg aagcgatgac
     7261 ggagcggccc cgagaggctc gcggcgaggc atgtacccac cgtgggatca ccgggcgggt
     7321 cgagcgccag ccgctggcgg tgcttcgcga ggacggcgtc gagcgccgac agcgtgatgc
     7381 ccgcctccac ggtgcaggtg aagtcgccgg gctcgtgctc gaggacgcgg ttcaacgccg
     7441 acgtgtcgag gtcgtcgccg atccgaacac ggcgaccggc agagtgcgcc gcggcgaggt
     7501 cggacgacgc agcggcgatc gacgccggcc gaagcacgct agaagcgttc ggcaacgcca
     7561 gtcctctcga gcgggtgcat gcggtagggg cccgggatct caccgcagag ccgcggcgtc
     7621 ggcacgacct ttcccgggtt ggcgagaccg tagggatcga acgcgcgcct gaggcgctcg
     7681 aacgtcgcga ggtcgttctc ggagaacatc ttgggcatcg agcaggcctt gtcgacgccg
     7741 acaccatgtt cgccggtcag tgagccgccg gcctcaagac acgccgcgag aatctcctct
     7801 gccagctcct tcgcccgctc ggcctcgcct gcggaggcgt cgtagaggac cagtgggtgc
     7861 aagtttccgt ctccggcgtg gaagacgttg ccgacgcgga gcccgtgctc ctcggagagc
     7921 tctccgatgc gacgaagaac ctcgggcagg cgcgtgcgcg ggacgactcc gtcctgcacg
     7981 tagtagtcgg gcgagactcg ccccatcgcc ggaaaggccc ccttgcgccc cttccacaga
     8041 agagcacgct cactctcgtc ctgggcaatc cgtacttcga aggcgccgtc ctcccggcag
     8101 atgcgctcca cctctgcggc gtcctcgtcg acctgaacgt ccacgccgtc gagctcgacg
     8161 aggagaacgg cctcggcgcc gtccgggtag cccgggtgga aggcggcctc ggcggcttcg
     8221 atcgtgagcc ggtccatcat ctcgatcgcg gcgggcagga tgcccgcccc gacgacgtgc
     8281 gagacggccg ttcccgccgc ctccggtgca cggaaggcgg caaggagcgt ggtcacccgc
     8341 tcgggggagc ggacgatgcg aagggtgatc ttggtcgcga ctcccagtgt cccttccgag
     8401 cccacgaagg cgccgagcag gtcgtagccc tccgtgtcga gcgccttgcc gcccagctcg
     8461 gcaacctcgc cgtcgggcag gacgagctcg aggccggtga cgtggtggac ggtgaacccg
     8521 ttcttcagac agtgagcgcc ccccgaattc tccgccacgt tgccgccgat ggtgcacacc
     8581 tgctggctcg acggatccgg agcgtagaag aagccgtcgc ctgccacggc gcgcgtcaca
     8641 tcgaggttcg tgacaccggg ctcgaccacg acgcgccgtg cgggcacgtc gacgtcgaga
     8701 atgcggttca tccgtgccac tgagatgaca atgccgtcgg cgaccggaag cgcaccgccg
     8761 gaaagtcccg ttccggcgcc gcgaaccacc cacggcacgc gctcgcggtg gcaggcgcgg
     8821 acgatacgtt gaacggcctc gaccgaatcg ggtagggcaa ccagctccgg ctgcgcgcgc
     8881 cagcccgtca agccgtcgca gtcataggtg cgaagctgct ccggctcggt gatcacgttc
     8941 cccggtccga gctcgcggcg aagctcctcc acggctgcgg cgctgagacc catccccttg
     9001 gatcctactc cgtgacgaat cgatgatcga tcattcacga cggttcccgg cccgcccgtc
     9061 gaggtgttct cataggatcc cgcgcatggt tcccatcgat cgtcccggaa agatcatctg
     9121 cgtcggcctg aactaccgcg accacgcaga ggagcagggt gttgagctgc ccgaggcgcc
     9181 actcttcttc gcgaaataca cgacgtctct gatcgggccc ggtgatccga tcgtcatccc
     9241 gccgatcgtc gagcagcccg actacgaggc ggagctcggc gtggtgatcg ggcaaacggt
     9301 gaaggggatc tcgcgcgaga acgcgatgga ggcagttgcc ggctacgtcg tcgccaacga
     9361 cgtctcggcg cgcgacctcc agttcgcgga cggacagtgg acgcgc
//
LOCUS       DBNGNK010000407         5378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_715659,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000407 DBNGNK010000000
VERSION     DBNGNK010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_715659"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1734)
                     /locus_tag="AC150V_12215"
     CDS             complement(<1..1734)
                     /locus_tag="AC150V_12215"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR3763726.1"
                     /translation="MAISAGVDTGGTFTDLVLHDDQTGEIRMAKVLSTPQEPSRAVFD
                     SFQKADVETREIASFVHGTTVATNALIERKGARVALLTTEGFRDILRIQRVSRPRHFD
                     LHWVKPRHFAPRSRCLGIPERVLRDGSVLIPLDEEEVRRQVARLRDGGDTDAVAVSYL
                     FSYVNPAHELRTRELIEEVWPGVQVSLSHEVLPRWREYERTSTTVIDAYLKPLMHDYM
                     AKLEQECDEAGIDQLLILRSNGGAIMSERAREQPVSLVRSGPSGGIMASAALGKALGL
                     GDLIACDMGGTSFEACLLPGSEPAFTNFEELEYGVPIALTMVDARAIGAGGGSLAWID
                     TAGILRVGPRSAGADPGPACYGRGGTQATVTDANAMLGRLAPEFLLAGDLELDVDAAA
                     SALDPLAEALGFSRERVAQGIVDVANSNMAQALRLVSTDRGYDPRGSTLVAYGGAGPL
                     HACELARALQIGRVAIPGYPGAFSAFGALLSDTRFDYTQTSWMRMRFLDLDRVNALFG
                     ELEQRAVEDFRREGFDEPPALVRSIDMRYVGQNWELSVSMAGGELTEADFDRAASLFE
                     EEHERFYGYSIP"
     gene            1854..2300
                     /locus_tag="AC150V_12220"
     CDS             1854..2300
                     /locus_tag="AC150V_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016325268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR3763727.1"
                     /translation="MSSSDVPAPSEGMAVTHFVVAADKERTARFYSDVLGGEMVFEAP
                     GAPTFVKLANTWVIVNEGGGPTPDKPEVVLETPPDPNRVSAFMNIRVADIEAVYEEWT
                     ARGAVFLTPPLDNHGYELRCYVQDPDGRIIEVGQATGMLEFFGLER"
     gene            complement(2304..2693)
                     /locus_tag="AC150V_12225"
     CDS             complement(2304..2693)
                     /locus_tag="AC150V_12225"
                     /inference="COORDINATES: protein motif:HMM:NF025384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HIR3763728.1"
                     /translation="MNATTAQGPLGQRRGTAFVVLIGIVTLGIYWIWWVYKTQEEMKQ
                     HTGEGLGGVLGLVVWIAVSAVSAFVIPSEVGNMYAKDGREKPITGWTGLWLFPFGLLI
                     IPIFVWFVRVQGALNRYWESKAASQTP"
     gene            2846..4171
                     /locus_tag="AC150V_12230"
     CDS             2846..4171
                     /locus_tag="AC150V_12230"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR3763729.1"
                     /translation="MRLTILHTNDIHGNVDGLARIATIVERIRAETDHPLVYVDAGDV
                     EDTTNRLSSITKGAAMHHLLGAAGCEAATVGNASWLRYGAHVLAEHAAAAPYPLLLAN
                     IRPVAGVRETAVLEAGDVRVGLIGVTAPFTRLIESYPFGVEALDPVPLVRRLAAALRD
                     DGADLVVLLSHLGLDVPESVVDDRRLLAKLEGDVDRVIGAHSHDALPDGERIAEVLVA
                     QTGWQGRYLGRIDIEEDGLSACLIPVSHETPPHPDVLAAAEAVERQAAAFLDEVIGEL
                     PRPLDTDAAAEWLAEIIRERMSADVAIIATGQAFAGSLRAGPLRRGDLWDACDSPSNP
                     AVTEMTGRQLGEVVARGNDPDLVHATPRPLRGRERGALQVAGPRGLDPAKTYRVAGTD
                     WELEPIGGLVDADWRLEVRLDFPTILREAIEEHLAGAAPSSNRRDIAIL"
     gene            4214..5362
                     /locus_tag="AC150V_12235"
     CDS             4214..5362
                     /locus_tag="AC150V_12235"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR3763730.1"
                     /translation="MRIGILTGGGDVPGLNACIKAAVNRVTGAGHEVVGIRRGWAAFL
                     HFDPDNPDSAAEWFEPLDPAAVRTIDRSGGTMLHTSRTNPGRVRTKEVPPHLADRASG
                     DGPYDFTEHALRVVEDRQLDALIAIGGDDTLSYALRLHEEGIPVIAIPKTMDNDVHGT
                     DYCIGFSTAITRGVQFIHNLRTSSGSHERLAIVELFGRYSGETSLITAYLAGVDRAVI
                     SEVPANVDRLAELLMTDKRANPSNYAMVTISEGAVLEGGELVASGEEDAYGHRKLGGV
                     GVQLGEILKASTGEDIIYQQLAYLMRSGSPDSLDLMVGTNYAVMAADLAVEGMHGRLV
                     ALRSGTYTSVPAGVIGEGVKRVDVDELYDVDEYQPKVRHVTGKPMFLY"
BASE COUNT          886 a         1869 c         1829 g          794 t
ORIGIN      
        1 ggggatcgag tagccgtaga agcgctcgtg ctcctcctcg aacagcgacg ccgcgcgatc
       61 gaagtcggct tcggtgagct cgccgccggc catcgagacg ctgagctccc agttctggcc
      121 cacgtaccgc atgtcgatcg agcgcacgag tgcgggcggc tcgtcgaatc cctcccggcg
      181 gaagtcctcg acggcccgct gctccagctc gccgaagagc gcgttcacgc gatccagatc
      241 gaggaagcgc atgcgcatcc aagaggtctg cgtgtagtcg aacctcgtgt cggagagcaa
      301 ggccccgaag gcggagaagg cccccgggta gcccgggatc gcaacccggc cgatctggag
      361 cgcccgcgcc agctcgcacg cgtggagagg cccggcgccg ccgtaggcga cgagcgtcga
      421 gccgcggggg tcgtagccgc ggtcggtcga cacgagccgc aacgcctgcg ccatgttcga
      481 gttggcgacg tcgacgatgc cctgagccac tctctcgcgc gagaagccaa gcgcttcggc
      541 cagcgggtcg agagcgctcg cggcggcgtc gacgtccagc tcgagatcgc cggcgaggag
      601 gaactccggc gccagccgcc cgagcatcgc gttcgcgtcc gtcacggtcg cctgagtgcc
      661 gccacggccg tagcaggcgg gcccggggtc ggcgccggca ctgcgcggcc cgactctgag
      721 gatgccggcc gtgtcgatcc aggcgaggct gccgccgccg gcgccgatcg cccgcgcgtc
      781 gaccatcgtc agcgcgatcg gtacgccgta ctcgagctcc tcgaagttgg tgaacgcagg
      841 ctcggagccg ggcagcaggc aagcctcgaa gctggtgccg cccatgtcgc acgcgatcag
      901 gtcgcccagg ccgagcgcct tgccgagcgc cgcggacgcc atgatgccgc ccgagggccc
      961 ggagcgcacg agcgagactg gctgctcgcg cgcccgctcc gacatgatcg cgccgccgtt
     1021 cgagcggagg atgagcagct ggtcgatgcc ggcctcgtcg cactcctgct cgagcttcgc
     1081 catgtagtcg tgcatcaacg gcttgaggta ggcgtcgatc accgtcgtcg aggtgcgctc
     1141 gtactcgcgc cagcgcggca gcacctcgtg cgagagcgag acctgcacac cgggccacac
     1201 ctcttcgatc aactcgcggg tgcggagctc gtgcgcgggg ttcacgtacg agaagagata
     1261 cgagacggct acggcatccg tgtccccgcc gtcgcgcagg cgcgcgactt gccggcgcac
     1321 ctcctcctca tcgagcggga tcagcaccga tccgtcgcgg aggacgcgct ccgggatccc
     1381 gaggcagcgc gagcggggag cgaagtggcg gggcttcacc cagtgcaggt cgaagtgcct
     1441 cggcctgctg acgcgctgga tgcggaggat gtcgcggaat ccctcggtcg tgagcagcgc
     1501 gacgcgtgcg cccttgcgct cgatcagcgc gttcgttgcg accgttgtgc cgtggacgaa
     1561 cgacgcgatc tcccgcgtct ccacatccgc cttctggaaa gagtcgaaga ccgcccgcga
     1621 cggctcctgc ggtgtcgaga gcaccttcgc catcctgatc tcgccggtct gatcgtcatg
     1681 caggacgagg tcggtgaagg tgccgcccgt gtccactcca gcgctgatcg ccatgcccgc
     1741 ctcctcgccg tcgccgaggc gcgatcctaa ttgtcgacaa tcagacaggt caaccgcctg
     1801 gcgtcctcta gcgtgagccg cggcagagcg acagcgaccc gaggagggcc acgatgagca
     1861 gcagcgatgt tccagctccg agcgaaggca tggcggtgac gcacttcgtc gtcgccgcgg
     1921 acaaagagcg aacggcccgc ttctactcgg acgtgctggg cggcgagatg gtcttcgagg
     1981 cccccggcgc gccgaccttc gtcaagctcg cgaatacctg ggtgatcgtc aacgaaggcg
     2041 gcgggccgac gccggacaag ccggaggtcg tgctggagac tcccccggat ccgaaccggg
     2101 tcagcgcgtt catgaacatc agggttgcgg acatcgaggc cgtgtacgag gaatggacgg
     2161 cacggggcgc agtcttcttg accccgccgc tcgacaacca cggctacgag ctgcgctgct
     2221 acgtgcagga tccggacggc cgcatcatcg aggtcggcca ggcgaccgga atgctcgagt
     2281 tcttcgggct cgaacgctga gcctcacgga gtctggcttg cggccttcga ctcccagtag
     2341 cggttcagag cgccctgcac gcggacgaac caaacgaaga tcgggatgat caggagcccg
     2401 aaggggaaga gccacagccc ggtccagccg gtgatcggct tctcgcgccc gtccttcgcg
     2461 tacatgttcc cgacctccga ggggatgacg aaggcgctca cggccgagac ggcgatccag
     2521 acgacgagcc cgaggacacc accgaggccc tccccggtgt gctgcttcat ctcctcctgc
     2581 gtcttgtaga cccaccagat ccagtagatc cctaaggtga cgatgccgat gaggacgacg
     2641 aaagcggtgc cccgcctctg tccgagcggc ccttgggcag tcgtggcgtt catagggtct
     2701 ccgttttcct cgcttcgctg gccgcgaatc agcctagtcg agcgatcggc gctcacaggg
     2761 aatcgcgcta agcgcaccat cgtgagatcg cgtacggacg ccactgcgag cggagcagaa
     2821 gacgcggccc gactagggtc tccgcgtgcg gctgacgatc ctgcacacga acgacatcca
     2881 cggaaacgtc gacgggctcg cccggatcgc cacgatcgtc gagcggatcc gcgccgagac
     2941 cgaccacccg ctcgtctacg tcgacgccgg tgacgtggag gacacgacga accgcctcag
     3001 cagcatcacg aaaggggcag cgatgcacca tctgctgggt gccgccggtt gcgaggccgc
     3061 caccgtcggc aacgcatcgt ggctccggta cggcgcgcat gtgctcgccg agcacgcggc
     3121 cgcggcgccg tatccgctcc tgctggcgaa catccggccc gtggcgggtg tccgggagac
     3181 ggcagtgctc gaggccggtg acgtccgcgt cggcctgatc ggcgtgaccg ctcccttcac
     3241 gcggttgatc gagagctacc cgttcggcgt cgaggcgctc gatccggtgc cgctcgtccg
     3301 ccggctcgct gctgcgctgc gagacgacgg cgctgatctc gtggttctcc tctcgcacct
     3361 cggcctcgac gtccccgagt cggtggtcga cgaccgccgg ctcctggcga agctcgaagg
     3421 cgacgtagac cgcgtcatcg gcgcccacag ccacgatgcg ctccccgacg gcgagcgcat
     3481 cgccgaggtg ctcgtcgccc agaccggctg gcaaggccgg tacctcggcc ggatcgacat
     3541 cgaggaagac ggcctgagcg cgtgcttgat tccggtttcg cacgagaccc cgccgcaccc
     3601 ggacgttctc gccgcagcgg aagccgtcga acgacaggcc gccgcctttc tcgacgaggt
     3661 gatcggcgag ctgccgcgcc cgctcgacac ggatgccgcc gcggagtggc tcgccgagat
     3721 catccgggag cgcatgtcgg ccgacgtcgc gatcatcgcg acgggacagg cattcgcggg
     3781 gagcctgcgc gccggcccgc tccggcgcgg cgacctctgg gacgcatgcg actcgccgtc
     3841 gaaccccgcc gtcacagaga tgaccgggcg gcagctcggc gaggtcgtcg cgcgcgggaa
     3901 cgaccccgat ctcgtccacg cgacaccaag gccgctgcga ggacgcgaac ggggagctct
     3961 ccaggttgcg gggccgcgcg gtctcgatcc cgcgaagacg taccgcgtcg ccggcaccga
     4021 ctgggagctc gagccgatcg gcggcctcgt cgacgccgac tggcggctcg aggtccgact
     4081 cgactttccc accatcctgc gcgaggcgat cgaggagcac ctcgcgggcg cggcgcccag
     4141 ttcgaaccgt cgggacatcg cgatcctgtg aacgctgcgc cgccgcggcg tgcggctgac
     4201 tagcatcgtc cggatgcgaa tcggaattct caccggcggc ggcgacgtgc cgggcctcaa
     4261 cgcctgtatc aaagcggctg tgaacagagt caccggcgcc ggccacgagg tggtcggcat
     4321 ccgtcggggc tgggctgcgt tcctgcactt cgacccggac aacccggatt ccgccgccga
     4381 gtggttcgag ccgctcgacc ctgcggccgt gcgcacaatc gaccgctccg gcggaaccat
     4441 gctgcacacc tccaggacga accccggccg ggtccgcacg aaggaggtgc cacctcacct
     4501 cgcggaccgc gcctcggggg acgggccgta cgacttcacc gagcacgcgc ttcgagtcgt
     4561 cgaagaccgg cagctcgacg cgctgatcgc tatcggcggc gacgacaccc tctcgtacgc
     4621 gttgcggctg cacgaggagg gcattccggt gatcgcgatt ccgaagacga tggacaacga
     4681 cgtccacggg accgactact gcatcggctt ctcgaccgcg atcacgcgcg gcgtccagtt
     4741 cattcacaac ctcaggacca gcagcggttc acacgagcgg ctcgcgatcg tcgagctgtt
     4801 cggccgctac agcggcgaga cgtcactgat caccgcctat ctcgccggtg tcgaccgggc
     4861 ggtgatctcc gaggtgcccg ccaatgtcga ccgcctcgcc gagctcctca tgaccgacaa
     4921 gcgcgcgaac ccctcgaact acgcgatggt cacgatctcg gaaggagcgg tgctcgaggg
     4981 cggtgagctc gtcgcctcgg gagaggagga cgcctacggc caccggaagc tcggcggtgt
     5041 cggtgtacag ctcggcgaga tcctgaaggc gtcgaccgga gaggacatca tctaccagca
     5101 gctcgcctac ctgatgcgct ctggcagccc ggactcgctc gacctgatgg tcggcacgaa
     5161 ctacgcggtg atggcggccg atctcgccgt cgaaggaatg cacgggcggc tcgtcgccct
     5221 ccggagcggc acgtacacca gcgttccggc cggcgtgatc ggggagggcg tcaagcgtgt
     5281 cgacgtcgac gagctgtacg acgtcgacga ataccagccc aaggtgcgac acgtcacggg
     5341 caagccgatg ttcctctatt agaaatggcc gtcctggg
//
LOCUS       DBNGNK010000408         3811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gaiella sp. isolate MAG05188 SRR25158358_k127_1289512,
            whole genome shotgun sequence.
ACCESSION   DBNGNK010000408 DBNGNK010000000
VERSION     DBNGNK010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414596
            Sequence Read Archive: SRR25158358
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gaiella sp. (soil metagenome)
  ORGANISM  Gaiella sp.
            Bacteria; Bacillati; Actinomycetota; Thermoleophilia; Gaiellales;
            Gaiellaceae; Gaiella.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/24/2026 19:44:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,451
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,398
            CDSs (with protein)               :: 2,398
            Genes (RNA)                       :: 25
            tRNAs                             :: 24
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 28
            CDSs (without protein)            :: 28
            Pseudo Genes (ambiguous residues) :: 0 of 28
            Pseudo Genes (frameshifted)       :: 2 of 28
            Pseudo Genes (incomplete)         :: 22 of 28
            Pseudo Genes (internal stop)      :: 5 of 28
            Pseudo Genes (multiple problems)  :: 1 of 28
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Gaiella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158358_k127_1289512"
                     /isolate="MAG05188"
                     /isolation_source="soil"
                     /db_xref="taxon:2663207"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.5 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      48..236
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            261..857
                     /locus_tag="AC150V_12240"
     CDS             261..857
                     /locus_tag="AC150V_12240"
                     /inference="COORDINATES: protein motif:HMM:NF025833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIR3763731.1"
                     /translation="MFRRILVLVALAGLLLVLAGVAVAADVTVRVEGKSRNIFGAVPA
                     KIEAATALQALDVASTRGEFHFVITSTSFGDYVSQIGKYAAGGSSGWVFKVNGESPPV
                     GADQAVLKDGDEVLWYWATFGDKGGPATLALKAAGGNCYTVSSLDDGGTSAPATAAKL
                     HVDGRRFKVDARGRGCVGKHRGNVRAFAVGAVRSNAVR"
     gene            854..1573
                     /locus_tag="AC150V_12245"
     CDS             854..1573
                     /locus_tag="AC150V_12245"
                     /inference="COORDINATES: protein motif:HMM:NF025833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HIR3763732.1"
                     /translation="MTLRRLLGLALAALALAGCGGAGSAGDDGSARLWVTRDRGDVVL
                     IDTSVAAGQTLMRALRSQADVETRYGGRFVQAIDGIEGDASARRDWFWFVNGLAGDRS
                     AAEYRLQDGDVAWWDYRDWTEDAEELQVVVGAFPEPFLHGYAGTVRPAVVRYAAQPLR
                     EPAERVARLIGAESVAPRGVAVPPDANVLRLVAGPEQLRAEPRQPGAGPESPVAFTFA
                     GDVDDLLAGPDGPFARRFELP"
     gene            1570..2271
                     /locus_tag="AC150V_12250"
     CDS             1570..2271
                     /locus_tag="AC150V_12250"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR3763733.1"
                     /translation="MSAVPAAVLLAAVAVAALLADRIWTVALLAAVLLAVCLRAPARR
                     RWPYLVGALVTGAGVALLSPLLWSSGGGTLLWDGPVVPVLGALDISTDEIAQALLSGL
                     RLTAVALAFSAYALLLDHDRLVAAAGFARRSVLAVALATRLVPTLERDAAGMAEAVRG
                     RGVELSGIRGYSRLLSPLVAGSLERATNLAEAMEARGYGRAGATRAPRPGWTRLDRSA
                     LALAALLVIAGAAWL"
     gene            2286..3659
                     /locus_tag="AC150V_12255"
     CDS             2286..3659
                     /locus_tag="AC150V_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR3763734.1"
                     /translation="MGHTYPGSAKPALDGVSIELRPGEVVALLGPSGSGKSTLLRALA
                     GLVPHFHGGGFRGSVTVGGLDTRSVRPAELAGIVASVFQDPEDQVVMAGVANEVAFGL
                     ENIGVEPAEIWPRVELALDGVDALHLAGRRTTELSGGELQRVCLAAAVALNPQLLLLD
                     EPTSQLDPDAAERFLGLLSGIGTAVVLSEQRVGRALSLAERVLFVEAACLVLDAPVAE
                     AEEWLAEHRPHYTREHAAELAAVHDVVHDDNIMATASDGTPSAPVVRLDGIRYGYPGG
                     PPVLVDTSLTLRPGEIVTLEGPNGSGKTTLAKIAAGLLDPDAGSVERVGRAAYLSQDP
                     GRYLMRETALDEVALAVDGDRGRALVALDRVELGWAADRHPRDLSSGERQRLALAAVA
                     VSEPDLLVLDEPTRGVDPEQKASLAEWVEDTAARGTAVLLATHDRAFAAHRRIQLGGL
                     PEVPVAV"
BASE COUNT          460 a         1281 c         1431 g          639 t
ORIGIN      
        1 acggccgccc aggccaagac cgggccccag cgccgggcga cggacgtccc catgcaccga
       61 tgataggctc ggctctcaca acggagcgcg gtgagggaac ccggtgcaag tccgggacgg
      121 tcccgccgct gtgaggggag atgctctccc gccgaggtgc cactggcctg ctggccggga
      181 aggcggtggg ggagggagcc ccgagtcaga agacctgcct cgctccctca tatatcgatc
      241 ccctcgcgga aggaggattc gtgttcagac ggattcttgt cctcgtagcc ctcgccgggc
      301 tgctcctcgt ccttgccggt gtcgcggtcg cagccgacgt caccgttcgc gtcgagggta
      361 agtcccgcaa catcttcggc gctgtgcccg ccaagatcga agcggcgacc gcgttgcagg
      421 cgctggacgt cgcgagcacg cgcggcgagt tccacttcgt gatcacgagc acgtcgttcg
      481 gcgactacgt cagccagatc ggtaagtacg ccgccggcgg cagctcgggc tgggtcttca
      541 aggtcaacgg cgagtcgcct cccgtcggag cagaccaggc cgtcctgaag gacggtgacg
      601 aggttctctg gtattgggcg accttcggag acaagggtgg gccggcgacg ctcgcgctga
      661 aggcggcggg tggcaactgc tacacggtga gctcgctcga cgacgggggc acgtccgctc
      721 cggcgacggc tgccaagctg catgtcgacg gacgtcgctt caaagtcgac gccagagggc
      781 gcgggtgcgt gggcaagcac cgcggcaacg ttcgcgcgtt tgcggtcggt gctgtccgct
      841 cgaacgcggt gaggtgactc ttcggcggct cctcggcctg gccctcgccg cgctggcact
      901 cgccggctgc ggtggggccg ggtcggccgg ggacgacggc tcggcgcgtc tctgggtgac
      961 gcgtgaccga ggtgacgtgg tgctcatcga cacgtccgtc gcggccggcc agacactcat
     1021 gcgggcgctt cggtcgcagg ccgacgtcga gacgcgctac ggcggccgtt tcgtgcaggc
     1081 gatcgacggc atcgagggcg acgcgtctgc gcggcgggac tggttctggt tcgtcaacgg
     1141 gctcgcgggc gaccgcagtg cggccgagta ccggctgcag gacggggacg tcgcctggtg
     1201 ggactaccgg gactggaccg aggacgcgga ggagttgcag gtcgtcgtcg gcgcctttcc
     1261 ggagccgttc ctccacggct acgcgggaac cgtgcgtccg gcggtcgttc gatacgcggc
     1321 gcagccgctg cgcgagcccg ccgagcgggt cgcccggctg atcggggccg agtcggtagc
     1381 gccgcgcggc gtggccgttc cccccgatgc gaacgtgctt cgactcgtcg ccgggccaga
     1441 gcagctccga gcagagccgc ggcagccggg ggccgggccc gagagtcccg tcgcattcac
     1501 cttcgccggc gacgtggacg acctgttggc cggcccggac gggcccttcg ctcgacgatt
     1561 cgagttgccg tgagcgccgt tcccgccgcc gtcctgctcg ccgcggtcgc cgtcgccgct
     1621 cttctggccg accggatctg gacggtggcg ctgctcgcgg ccgtcctcct cgcggtctgc
     1681 ctgcgcgcac cggcgaggcg tcgttggccc tatctcgtcg gtgccctcgt gaccggcgcc
     1741 ggcgtcgcgc tcctctcgcc gctgctctgg tcgtccggcg gcgggacact tctctgggac
     1801 gggccggtcg ttccggtgct cggtgcgttg gacatctcca ccgacgagat cgcgcaggcg
     1861 ctgttgagtg gacttcggct gacggccgtg gctctcgcat tctcggcgta tgcgctgttg
     1921 ctcgatcacg atcgcctcgt cgcggcggcc ggtttcgcac ggaggtcggt tctcgctgtg
     1981 gctcttgcga cgcggctcgt tccgacgctc gagcgggacg ccgcggggat ggccgaggca
     2041 gtgcggggcc gcggtgttga gttgtcgggc attcgaggct actcgcggct tctctcgcct
     2101 ctggtggcgg ggtcgctgga gcgcgcgacg aacctcgccg aggcgatgga ggcacggggc
     2161 tacggacgcg ccggcgcgac gcgcgcccca cgtcccggct ggacgcgact cgatcggagt
     2221 gctcttgcac tggcagctct cctggtgatc gcgggggcag cgtggctcta gcagttgctg
     2281 ctggggtggg gcacacctat ccgggctcgg cgaagcccgc gctggacgga gtgtcgatcg
     2341 agctccgccc gggcgaggtc gttgctctgc tcggcccgtc cgggtcgggc aagtcgacgc
     2401 tgctgcgcgc gctcgccggt ctcgtcccac acttccacgg cggcggcttc cgcggttcgg
     2461 tcaccgtcgg cgggctcgac acgcgatcgg ttcgccctgc cgagctggcg ggaatcgttg
     2521 cctcggtctt ccaggatccg gaggatcagg tcgtgatggc aggggttgcg aacgaggtgg
     2581 ctttcgggct cgagaacatc ggtgtcgagc ctgcagagat ctggccgcgg gtcgaactgg
     2641 ccctcgacgg tgtcgatgcc ctgcatctgg cggggaggcg cacgaccgag ctgtcgggcg
     2701 gtgagttgca gcgcgtctgc ctcgccgcgg cggtcgcact gaatccgcag ctgttgctgc
     2761 tggacgagcc gacatcgcag ctcgacccgg acgctgcgga gcggtttctc ggcctcctga
     2821 gcggcatcgg gacggctgtc gtgctgtccg agcagcgggt cggccgagcg ctgtccttgg
     2881 ccgagcgcgt gctcttcgtg gaggccgcgt gcctcgtgct ggacgccccg gtcgcggagg
     2941 cagaggagtg gcttgcggag catcgcccgc actacacgcg tgagcacgcg gcggagcttg
     3001 cggctgtaca tgatgttgtc catgatgaca acatcatggc gactgcctcg gacggaacgc
     3061 cctctgcgcc ggtcgtgcgg ctcgacggga ttcgctacgg ctatccgggc gggccgccgg
     3121 tgctcgtgga cacgtccctc acgcttcggc ccggggagat cgtcacgctg gaggggccga
     3181 acggctccgg caagacgacg ctcgcgaaga tcgcggccgg cctcctcgat cccgatgcgg
     3241 gatccgtcga gcgcgtcggc cgggccgcat acctgtcgca ggatccaggc cggtatctga
     3301 tgcgcgagac tgcgctcgac gaggtcgccc tcgcggtcga cggcgaccgc ggccgggccc
     3361 tcgtggcgct cgaccgagtg gagctcgggt gggcggcgga ccgccatccg cgcgacctct
     3421 cgagcggtga gcgtcagcga ctcgcgctcg ccgccgtcgc ggtctcggaa cccgacctcc
     3481 tcgttctcga cgagccgacg cgcggtgtcg atccggagca aaaggcctcg ctcgccgagt
     3541 gggtggagga caccgcggct cgtggaaccg ccgtgctgct cgcgacccac gaccgtgcgt
     3601 ttgccgcgca tcggcggatc cagctgggcg gactgccgga ggtgccggtt gccgtctagg
     3661 tgggtcgctc tcgcagctgc ggcggcgctt gccgccgccg tctgggccgc gctcgacccg
     3721 gcacgcgggg ggctcgcggt tctcctcgca gcggtcgcgg tgatcggcgc cggatttgcc
     3781 tggttcgagg gaaaggccgt caccgctcac g
//
